NetStart 1.0 Prediction Results

Processing locus 1: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTAGTTTTAGAAGTTATGTGTTTGGTTTAGTTCGATGTTGTGATTCTGGGTGATTGATTAGCTTGTCTCGTCGTATGTTG GAATTTATCTATGGATTATGAAAATGGGTTTTTAGTTTAGCATCTATGAGAGCAGCAATGGTAGCTGTTACTGGAGAAGT ACTGCATCTGCTTCGGAGGTATTTGGGTGAATATGTGCATGGACTCTCCACAGAGGCTCTGAGAATTAGTGTCTGGAAAG GTGATGTTGTTCTTAAAGATTTGAAGTTAAAGGCGGAAGCTCTTAATTCACTGAAGCTTCCAGTAGCTGTCAAATCTGGT TTTGTTGGAACCATCACTTTGAAGGTCCCTTGGAAAAGTTTAGGCAAAGAGCCAGTCATTGTTCTGATCGATCGTGTCTT CGTTCTTGCTTATCCTGCCCCTGATGACCGAACACTTAAGTTTTTCACTTTGGTTGGTACTGAATTTGCTTACACTAATT ACATACCAGGAGGAAGACAGGGAAAAGCTTCTAGAAACCAAGCTTCAGCAGATCGAGGTACTTCGTATTTCTGGCTGATG GAGCTTCATGGATACGAAGCAGAAACAGCAACTCTTGAAGCAAGGGCAAAGTCTAAGCTGGGAAGTCCTCCACAAGGGAA TTCATGGCTAGGCTCAATTATTGCTACAATTATTGGTAATCTGAAAGTATCCATCAGCAATGTACATATAAGATACGAGG ATTCTACCAGAGACAGCTCAGAAATACTTGCTAGCTTTTTTTCTTATTTCAACAATATTTGCTCTAGTAATCCAGGGCAC CCCTTTGCTGCGGGCATCACCTTGGCCAAGCTCGCAGCTGTTACAATGGATGAAGAAGGAAATGAAACCTTTGACACAAG TGGCGCTTTGGATAAACTGCGTAAGTCGTTGCAGCTTGAGAGGCTCGCTCTGTATCATGATTCTAATAGCTTTCCCTGGG AAATAGAGAAGCAATGGGACAATATTACTCCTGAGGAATGGATTGAGATGTTTGAAGATGGCATAAAGGAGCAGACCGAG CATAAAATAAAGTCAAAATGGGCCTTGAATCGTCACTATCTATTGTCCCCTATCAATGGATCTCTTAAATATCACCGGTT AGGAAATCAGGAGAGAAATAATCCGGAGATTCCATTTGAAAGGGCATCAGTTATCCTTAATGATGTTAACGTGACTATCA CAGAGGAGCAATATCATGATTGGATAAAGCTAGTCGAAGTTGTCTCCAGATACAAAACATATATAGAGATTTCACATTTA AGGCCTATGGTTCCTGTTTCAGAAGCTCCACGTCTGTGGTGGCGGTTTGCTGCTCAAGCTAGTTTACAACAAAAGAGATT ATGGTACACGCGCTATATTCAGCTGTATGCCAATTTTCTGCAGCAGTCATCAGATGTTAATTACCCAGAGATGAGAGAAA TCGAAAAGGACCTTGATTCCAAAGTGATTCTTTTGTGGAGGTTGCTTGCACATGCAAAGGTAGAATCTGTAAAGTCAAAA GAAGCAGCTGAGCAGAGAAAGCTGAAAAAGGGTGGTTGGTTCTCATTCAACTGGCGGACAGAAGCTGAAGATGACCCTGA GGTAGATAGTGTGGCTGGCGGATCAAAATTGATGGAGGAAAGATTGACTAAAGATGAATGGAAGGCCATAAATAAGTTAC TGAGTCATCAGCCAGATGAAGAGATGAATTTGTATTCAGGAAAAGACATGCAGAATATGACTCACTTTCTTGTAACGGTA TCAATTGGTCAAGGTGCGGCTAGGATTGTTGATATAAATCAGACTGAGGTGCTGTGTGGTAGGTTTGAGCAGCTCGATGT GACGACCAAGTTCAGACATCGAAGTACTCAGTGTGATGTATCACTGAGGTTCTATGGCTTATCTGCACCAGAAGGTTCTC TAGCGCAGAGTGTATCCAGTGAGAGAAAGACCAATGCCTTGATGGCTAGTTTTGTGAACGCACCAATTGGAGAGAACATT GATTGGAGGCTATCAGCCACGATATCCCCTTGTCATGCTACGATATGGACTGAAAGTTATGATCGTGTATTAGAGTTTGT GAAGCGAAGTAATGCAGTTAGCCCTACTGTTGCGCTGGAAACTGCTGCTGTGCTTCAGATGAAACTAGAGGAAGTGACTC GACGGGCTCAAGAGCAACTTCAAATAGTATTGGAGGAGCAAAGCAGATTTGCTCTCGACATTGATATTGATGCTCCAAAG GTGAGAATTCCTTTGAGGGCTTCAGGATCGTCCAAGTGCAGTAGCCATTTCCTATTGGATTTTGGTAATTTTACGTTAAC CACCATGGATACTCGGTCTGAGGAGCAGAGGCAAAACTTATATTCCCGGTTCTGCATTTCGGGAAGAGATATTGCTGCTT TCTTTACAGACTGTGGATCTGACAACCAAGGTTGCAGTTTAGTCATGGAAGATTTCACTAATCAGCCAATTCTGTCTCCC ATTCTAGAGAAAGCGGACAATGTTTATTCCCTTATTGATAGGTGTGGAATGGCAGTTATTGTTGATCAGATCAAAGTACC TCACCCAAGTTACCCATCTACTAGAATATCGATCCAAGTGCCAAATATTGGTGTTCATTTCTCTCCAACAAGATATATGA GGATCATGCAATTGTTCGACATCTTGTATGGTGCTATGAAAACCTACAGTCAAGCTCCTGTTGATCACATGCCAGATGGA ATCCAACCTTGGAGTCCTACAGATCTTGCTAGTGATGCTAGAATCTTAGTTTGGAAGGGAATTGGCAACTCTGTTGCTAC TTGGCAATCTTGTCGCCTTGTTCTATCTGGTTTATATCTGTATACATTTGAATCAGAGAAGTCACTTGATTATCAACGTT ATTTATGCATGGCTGGTAGGCAAGTATTCGAGGTTCCACCCGCAAATATTGGTGGTTCTCCATACTGTCTTGCTGTCGGT GTAAGGGGAACAGATCTTAAAAAGGCCCTGGAATCTTCTAGCACCTGGATCATAGAATTTCAGGGGGAGGAGAAGGCAGC TTGGCTGAGAGGACTGGTCCAAGCCACCTATCAAGCTTCTGCTCCTTTGTCGGGGGATGTACTTGGTCAAACGAGTGATG GTGATGGTGACTTCCATGAACCTCAGACCAGGAATATGAAAGCTGCTGATCTTGTTATTACTGGTGCTTTGGTTGAGACG AAGTTGTACTTGTATGGAAAGATCAAGAATGAATGCGATGAGCAGGTTGAAGAGGTTCTGCTTCTTAAAGTTCTTGCTTC CGGAGGAAAGGTGCACTTGATAAGTTCCGAGAGTGGCTTAACTGTTAGAACAAAGTTGCACTCTCTGAAAATCAAAGATG AACTCCAGCAGCAGCAGTCAGGAAGTGCTCAGTATTTAGCTTACTCGGTCCTGAAGAACGAAGACATACAAGAATCATTA GGAACATGTGATTCTTTCGACAAGGAGATGCCTGTAGGACATGCAGATGATGAAGATGCCTACACAGATGCTTTACCAGA GTTTCTGTCACCTACAGAACCAGGTACTCCGGATATGGATATGATTCAATGCAGCATGATGATGGACTCCGATGAACATG TGGGACTCGAGGATACTGAAGGAGGGTTTCATGAAAAAGATACATCTCAGGGAAAAAGTCTTTGCGATGAGGTATTTTAT GAGGTGCAGGGTGGTGAATTTTCTGATTTTGTGTCAGTTGTTTTCTTGACGAGGAGCTCCAGTTCCCACGATTATAATGG AATTGATACACAGATGAGCATTCGCATGTCAAAACTGGAGTTCTTTTGCAGCAGACCAACTGTTGTTGCTCTAATTGGTT TTGGATTTGATTTGAGCACTGCATCATATATCGAAAATGATAAAGATGCCAACACTTTGGTACCTGAGAAATCTGATAGT GAGAAAGAAACAAATGATGAAAGTGGGCGCATTGAAGGCTTACTTGGATATGGCAAGGATCGTGTAGTGTTTTACCTGAA CATGAATGTTGATAATGTGACTGTGTTTCTCAACAAGGAGGATGGTTCTCAGCTTGCGATGTTTGTGCAGGAACGATTTG TGTTGGACATTAAGGTTCACCCGAGCTCTCTTTCAGTAGAAGGGACTCTGGGAAATTTTAAACTCTGTGACAAGTCGTTA GACTCTGGAAACTGTTGGAGTTGGCTCTGTGACATACGAGATCCTGGTGTTGAGTCTCTTATCAAGTTCAAATTCAGTTC ATATAGTGCTGGCGACGACGACTATGAAGGATATGATTATAGCTTATCTGGTAAACTATCGGCTGTTCGCATTGTATTTC TCTACAGATTTGTTCAAGAGGTTACAGCATATTTCATGGGACTTGCTACTCCTCATTCTGAAGAAGTCATTAAACTTGTT GACAAAGTTGGAGGCTTTGAGTGGCTAATCCAGAAAGATGAAATGGATGGTGCTACTGCGGTGAAACTGGACCTTTCGTT AGACACTCCAATAATTGTTGTCCCTAGAGATTCTCTGAGCAAGGATTACATTCAACTTGACCTTGGTCAGCTGGAGGTAT CAAATGAAATAAGTTGGCATGGGTGTCCGGAGAAAGATGCCACTGCTGTACGTGTAGATGTTCTTCATGCGAAGATTCTT GGACTAAATATGTCGGTAGGCATAAATGGTTCAATTGGTAAACCAATGATACGTGAAGGACAGGGATTGGACATTTTTGT CCGGCGCAGCCTAAGAGATGTTTTTAAGAAAGTCCCAACACTTTCGGTTGAGGTTAAGATTGATTTCTTGCATGCTGTGA TGTCTGACAAAGAATATGATATTATTGTAAGTTGCACATCCATGAATTTGTTTGAGGAGCCAAAGCTACCTCCAGATTTC CGTGGTAGCAGTTCTGGTCCCAAAGCTAAAATGCGTCTACTGGCTGACAAAGTCAACTTGAATAGTCAGATGATTATGTC ACGTACAGTTACCATACTGGCTGTTGATATCAATTATGCTCTTCTCGAGTTACGTAATAGTGTTAATGAAGAGTCATCAT TAGCTCATGTTGCGGTACGTGCTTCTGAGCCTAATTCTTCTATTTCCTGGATGACATCACTATCTGAGACAGACTTGTAT GTGTCAGTTCCTAAAGTTTCTGTTCTTGATATCCGCCCTAACACAAAGCCTGAAATGCGACTGATGCTCGGCTCATCGGT CGATGCTTCCAAGCAGGCTTCTTCTGAATCTCTTCCCTTTTCCCTTAATAAGGGAAGCTTTAAGCGAGCCAACTCTAGGG CTGTTCTTGATTTTGATGCACCATGTTCAACGATGTTGCTGATGGATTATAGATGGCGAGCATCCTCTCAGTCATGTGTT TTGCGGGTGCAACAACCTCGTATCCTTGCTGTTCCTGACTTCCTTCTTGCGGTTGGTGAATTTTTTGTTCCAGCTTTGCG TGCAATAACAGGAAGAGATGAAACATTAGATCCAACAAATGATCCTATCACCAGAAGTAGAGGCATAGTTCTCTCTGAGC CACTCTATAAACAGACAGAAGATGTTGTGCACTTGTCCCCACGTAGACAATTAGTAGCAGATTCACTGGGCATTGATGAG TATACCTATGACGGATGCGGAAAGGTAATCTCGTTGAGTGAGCAAGGAGAGAAGGATTTAAACGTAGGAAGATTAGAACC CATCATAATCGTTGGGCATGGAAAGAAGCTGCGCTTCGTCAATGTGAAAATTAAGAATGGATCGCTTCTGAGCAAGTGCA TTTATTTGAGTAACGATAGTAGCTGTTTATTTTCTCCGGAAGATGGTGTTGACATCTCAATGTTAGAAAATGCCTCATCT AATCCGGAGAATGTGCTTAGCAATGCGCACAAATCATCAGATGTTTCAGACACTTGTCAATATGATTCGAAGTCTGGTCA GAGTTTTACCTTTGAAGCTCAGGTTGTATCTCCTGAGTTCACGTTCTTTGACGGTACAAAGTCATCTCTGGATGACTCTT CTGCTGTAGAAAAGCTTTTGCGAGTGAAATTGGATTTTAATTTTATGTATGCCTCAAAAGAAAAAGACATCTGGGTTCGA GCCTTGCTAAAGAACCTGGTTGTTGAAACTGGGTCTGGTCTTATCATTCTTGATCCAGTCGATATCTCTGGGGGATACAC TTCTGTTAAAGAGAAAACAAATATGTCCTTAACCTCCACTGACATATACATGCACCTTTCTTTGAGTGCTCTTTCTCTCC TACTGAATCTGCAGAGCCAAGTAACTGGAGCTTTACAATCTGGAAATGCTATTCCTTTAGCTTCATGCACCAACTTTGAC AGGATTTGGGTCTCACCAAAAGAAAATGGACCTAGGAACAACCTCACAATTTGGAGGCCGCAGGCTCCTTCAAACTATGT CATATTGGGAGACTGTGTGACGTCAAGGGCAATTCCTCCTACACAAGCTGTAATGGCAGTAAGTAATACATATGGGCGTG TGAGGAAGCCGATTGGGTTTAATCGTATTGGCCTGTTCTCTGTTATTCAAGGTTTAGAAGGGGATAATGTTCAGCATTCC CATAATAGCAATGAGTGTTCCCTCTGGATGCCTGTAGCTCCTGTAGGGTACACTGCTATGGGTTGTGTTGCAAATATAGG AAGTGAGCAACCACCTGATCACATTGTTTATTGTCTAAGCATTTGGCGTGCTGATAATGTTCTTGGAGCATTCTATGCTC ACACTTCAACTGCAGCTCCTTCCAAAAAATATAGCCCTGGCCTCAGTCATTGTCTTCTTTGGAATCCCCTTCAGTCCAAG ACATCTTCATCCTCTGACCCATCATCGACAAGTGGGTCCCGGAGTGAGCAATCAAGTGATCAGACTGGAAATTCATCAGG ATGGGACATTCTCAGGTCAATTTCTAAGGCAACTAGTTATCATGTTTCAACTCCTAATTTTGAGAGAATCTGGTGGGACA AAGGTGGTGATCTTCGAAGACCTGTCTCAATATGGAGGCCTGTTCCACGTCCTGGTTTTGCAATATTGGGTGATAGCATA ACTGAAGGATTGGAGCCACCTGCACTGGGAATACTCTTCAAAGCAGATGACTCTGAGATTGCTGCAAAACCTGTACAGTT CAATAAAGTCGCACATATTGTGGGGAAAGGTTTCGATGAAGTTTTCTGCTGGTTTCCTGTTGCACCTCCTGGTTATGTTT CTCTAGGATGTGTTCTCTCTAAATTTGATGAAGCGCCTCATGTCGATTCGTTTTGTTGTCCAAGGATTGATCTTGTTAAC CAGGCCAACATTTATGAAGCGTCTGTCACGAGATCTTCAAGTTCAAAGTCCTCTCAACTCTGGAGCATTTGGAAAGTTGA CAATCAGGCATGTACTTTTCTTGCACGTTCTGATCTTAAAAGACCTCCAAGTCGAATGGCTTTCGCTGTTGGAGAATCTG TAAAGCCGAAGACACAGGAAAATGTAAATGCAGAAATTAAGCTGAGATGCTTCTCCCTGACACTTCTAGATGGATTACAT GGAATGATGACTCCTCTCTTTGACACAACTGTCACCAATATCAAGCTAGCAACTCATGGTCGTCCTGAGGCCATGAATGC AGTACTCATTTCCTCAATTGCTGCTTCAACATTTAACCCGCAGTTGGAAGCGTGGGAGCCCCTTCTGGAGCCATTTGATG GAATATTCAAGTTGGAAACATATGATACTGCTTTGAACCAATCGTCAAAGCCAGGAAAACGGCTGCGAATTGCTGCTACT AACATTTTGAACATAAATGTTAGTGCTGCAAACCTTGAAACTCTTGGCGATGCCGTTGTTTCCTGGAGAAGGCAATTAGA ACTTGAAGAAAGAGCGGCCAAAATGAAAGAAGAATCAGCTGCCTCTCGTGAGAGTGGGGATCTTTCAGCTTTTTCAGCTC TTGATGAAGATGATTTTCAGACGATTGTGGTAGAGAATAAACTCGGCCGTGACATTTATCTGAAAAAGCTGGAGGAGAAC TCAGATGTAGTTGTGAAATTGTGTCACGATGAAAACACTTCTGTCTGGGTTCCCCCTCCAAGGTTCTCCAATCGGTTAAA TGTTGCAGACAGTTCTAGAGAAGCACGCAATTACATGACTGTACAGATTCTTGAGGCTAAGGGTTTGCATATTATTGATG ATGGTAACAGTCACAGTTTCTTTTGCACCCTACGCCTCGTTGTTGATAGTCAGGGTGCAGAACCACAAAAGCTTTTTCCT CAAAGTGCTAGGACAAAGTGTGTCAAGCCTTCCACTACAATTGTCAACGACTTGATGGAGTGCACTTCCAAGTGGAATGA ACTTTTTATTTTTGAAATTCCACGGAAGGGAGTTGCCAGATTGGAGGTTGAGGTCACAAACCTTGCTGCTAAAGCTGGAA AAGGGGAGGTCGTCGGGTCACTTTCGTTTCCTGTTGGACATGGGGAAAGTACGCTTAGAAAGGTAGCTTCAGTAAGGATG CTACATCAATCAAGTGATGCCGAGAATATAAGTTCGTATACGCTTCAGAGAAAGAATGCTGAAGATAAACATGACAACGG GTGCTTGCTTATCTCAACATCTTACTTTGAGAAGACGACAATCCCGAACACACTAAGAAATATGGAAAGTAAGGATTTTG TTGACGGAGACACTGGTTTCTGGATCGGGGTTCGTCCTGATGACTCATGGCATAGCATTCGCTCACTGCTTCCGCTCTGT ATTGCTCCGAAATCACTACAAAATGATTTTATTGCAATGGAAGTTTCTATGAGAAATGGGAGAAAGCACGCAACATTCAG ATGTTTAGCTACAGTTGTAAATGATTCAGATGTCAATTTAGAGATATCTATATCTTCCGATCAGAATGTCTCTTCTGGAG TAAGCAATCATAATGCTGTGATTGCCTCTAGATCTTCATATGTTTTGCCATGGGGATGTTTATCAAAGGATAATGAACAG TGCCTGCATATCCGGCCAAAGGTTGAAAACTCTCATCATTCTTATGCATGGGGTTATTGCATCGCAGTGAGTTCTGGTTG CGGAAAAGATCAACCATTTGTTGATCAAGGTTTACTGACTCGGCAGAATACAATCAAACAAAGCAGCAGGGCATCTACAT TTTTTTTGAGATTAAACCAGCTTGAGAAGAAGGATATGCTTTTCTGCTGCCAGCCTTCTACTGGGAGTAAGCCATTATGG CTCAGTGTAGGAGCAGATGCGTCAGTTCTTCATACAGACCTCAATACACCAGTTTATGATTGGAAAATTTCTATTAGTTC TCCTTTAAAGCTAGAAAATAGACTTCCATGTCCTGTCAAATTCACAGTGTGGGAAAAGACAAAAGAAGGCACATATCTCG AGAGACAACATGGTGTAGTATCCTCAAGGAAGAGTGCTCATGTATATTCAGCAGATATTCAGAGACCTGTATATCTTACG TTAGCTGTCCATGGCGGTTGGGCTTTGGAAAAGGATCCTATTCCTGTTTTAGATATATCTTCAAACGACAGCGTGTCATC ATTTTGGTTCGTCCATCAACAGAGCAAAAGGAGATTGCGTGTAAGTATAGAACGTGATGTGGGAGAAACAGGGGCTGCAC CAAAGACTATAAGGTTCTTTGTCCCATACTGGATTACAAACGATTCCTATCTTCCACTAAGCTATAGAGTGGTGGAGATT GAACCTTCAGAAAATGTGGAAGCTGGCTCCCCCTGTCTGACTAGGGCTTCCAAATCTTTTAAGAAAAATCCTGTTTTTTC GATGGAGAGAAGACACCAGAAGAAAAATGTCCGAGTGCTTGAGTCTATTGAGGATACTAGTCCTATGCCTAGCATGCTTT CTCCACAAGAATCAGCTGGCCGGAGTGGCGTTGTACTGTTTCCGTCTCAGAAGGATTCTTATGTCTCTCCACGAATTGGT ATTGCTGTCGCAGCTAGGGATTCTGATAGTTACAGTCCTGGAATTTCTCTCCTTGAGCTTGAGAAGAAGGAACGCATAGA TGTAAAGGCATTCTGCAAAGACGCCTCATACTACATGCTCTCGGCAGTATTAAACATGACATCTGACAGAACAAAGGTTA TTCATCTCCAACCACACACCTTATTTATCAACAGGGTGGGCGTGAGCATCTGTCTTCAACAGTGCGATTGTCAGACAGAA GAATGGATTAATCCTTCTGATCCTCCGAAACTCTTTGGATGGCAGTCTTCCACCCGGCTTGAGTTACTGAAATTACGAGT GAAGGGATATAGATGGAGCACGCCATTCAGCGTCTTTAGCGAAGGTACAATGCGTGTTCCTGTTCCAAAAGAAGATGGGA CTGATCAATTGCAGCTAAGAGTACAAGTCAGAAGCGGGACAAAGAACTCACGTTATGAAGTTATCTTCAGGCCGAACTCT ATCTCTGGCCCTTACAGGATCGAAAACCGTTCCATGTTTCTTCCTATTCGCTATCGCCAAGTGGAAGGTGTCAGCGAGTC TTGGCAATTTCTTCCTCCTAATGCGGCTGCTTCGTTTTATTGGGAAAATCTTGGTAGACGACATCTATTTGAACTTCTTG TTGATGGAAACGATCCTTCTAATTCAGAAAAGTTCGATATTGATAAGATAGGTGATTATCCACCACGAAGTGAAAGTGGA CCTACCAGACCCATACGAGTTACTATTTTAAAGGAAGACAAAAAGAATATTGTCAGAATCAGTGACTGGATGCCAGCAAT TGAGCCTACTTCAAGCATAAGCAGAAGACTTCCAGCATCATCCCTGTCAGAATTATCTGGAAATGAGTCTCAGCAATCTC ATCTTTTGGCCTCAGAAGATTCTGAGTTTCATGTTATTGTTGAGCTTGCTGAACTTGGAATCTCCGTAATCGACCATGCT CCTGAGGAAATACTGTACATGTCAGTGCAAAACCTCTTTGTGGCATATTCAACCGGCTTGGGCTCTGGACTTAGCAGGTT CAAACTAAGGATGCAAGGAATACAAGTTGATAACCAATTGCCATTGGCTCCAATGCCAGTTCTGTTCAGACCACAGCGAA CCGGAGATAAAGCTGATTATATTTTGAAGTTTTCTGTTACTTTGCAATCAAATGCTGGCCTTGATCTCCGTGTCTACCCG TATATAGATTTCCAAGGACGTGAAAATACTGCCTTTCTGATAAACATCCATGAACCTATTATCTGGCGCATTCATGAGAT GATCCAGCAAGCCAACTTGAGTAGACTATCCGATCCTAACTCGACGGCTGTATCAGTTGATCCATTCATTCAAATCGGAG TTCTTAACTTCTCGGAAGTTAGATTTAGAGTATCCATGGCGATGTCCCCCAGTCAAAGGCCGAGGGGAGTGCTTGGATTT TGGTCATCCCTGATGACTGCTCTTGGGAATACAGAAAACATGCCAGTACGCATAAGTGAGAGATTTCATGAGAACATATC CATGCGCCAAAGTACTATGATCAATAATGCTATTAGGAACGTAAAGAAAGACCTCCTTGGCCAGCCTCTTCAGTTGCTCT CAGGTGTTGATATTCTTGGCAATGCAAGCAGCGCGCTTGGACATATGAGTCAAGGCATAGCTGCATTATCAATGGATAAA AAATTCATCCAAAGTCGACAGAGACAGGAAAACAAAGGTGTTGAGGACTTTGGCGATATTATCAGAGAAGGAGGTGGAGC TCTAGCGAAAGGCCTGTTTAGAGGAGTCACAGGCATATTGACAAAGCCTCTCGAAGGTGCAAAGTCTTCTGGTGTCGAAG GCTTTGTCTCTGGGTTTGGGAAAGGAATCATTGGTGCTGCTGCCCAGCCTGTGAGCGGAGTTCTGGATCTTCTGTCAAAG ACCACTGAAGGTGCAAATGCCATGAGGATGAAAATAGCAGCTGCCATCACCTCAGATGAACAACTTCTTCGCCGGAGACT TCCAAGAGCGGTTGGTGCTGATAGCTTGCTTCGTCCTTACAATGATTACAGGGCACAGGGGCAGGTCATATTGCAGTTGG CAGAATCTGGATCATTCCTGGGCCAGGTTGATCTGTTTAAAGTACGTGGGAAGTTTGCATTAACAGATGCCTACGAAAGT CATTTCATCCTACCAAAAGGAAAAGTTCTGATGATCACACATCGAAGAGTGATATTGCTACAACAACCCTCCAACATAAT GGGCCAAAGAAAGTTTATTCCGGCAAAAGATGCGTGTTCTATACAGTGGGATATTCTATGGAATGATCTGGTAACCATGG AACTGAGTGACGGGAAAAAAGACCCACCTAACTCTCCACCTTCGCGGCTTATTCTCTATCTGAAAGCAAAGCCACATGAC CCGAAGGAACAATTCCGTGTTGTCAAATGCATTCCCAACAGCAAGCAGGCCTTTGATGTTTACTCAGCTATCGATCAAGC CATTAACTTGTATGGGCAGAATGCTTTAAAGGGAATGGTGAAAAACAAAGTGACAAGACCATATTCCCCAATTTCTGAGA GTTCTTGGGCTGAAGGAGCTTCTCAACAAATGCCAGCTTCAGTTACACCATCTTCAACCTTTGGCACTAGCCCTACGACC AGCTCAAGTTAAAGTCTGAATCTTTGAGCTCTCATTCCTTCCAATGTCCCCTCTCGCCTCCCCGGTAAAGACTTTTTCCT CTTCTCCATTTGCTTGAACAAAGCTTTATTT ...............i..................N.......................................N..... ..........N......N.....i.....................N...........i...................... ................................N.....N......................................... ...N............................................................................ ................................................................................ .......................N........................................................ .............................................................................N.. .......N........................................................................ 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N...................N........N...................................N.............. ............N..........................i.........N.....N................N....... ...........................................i...N................................ ................................................................................ ...................................N........................................N... ................N......................................N........................ ...........................N.................................................... .........N.............................N........................................ ..........N..................................................................... ........................................................N....................... ................................................................................ .............i.................................................................. ............................................................N................... ...............................................................................N ..................................N....................i........................ ................................................................................ ..i...................................N......................................... ............i....................................N........................i..... ......................................................i......................... .................................N.............................................. ....................N........................................................... ...i............................................................................ .....................................................................N.......... ..............................................................i................. ..............................N............................................N.... ..................N............................................................. ..........N.........................................i........................... ...................................................i............................ .................................................N.......................N....N. ................................................................................ ...................................N.....i...................................... ............i..........................i...........................N............ .....................N......................i..........................i........ ................................................................................ ................................................................................ ................................................................................ ................N....i.....N...........................N........................ .........................................N...................................... ..................................................................i............. ..............................N...............................................i. .............................N...........................i....N.............N... ...........................................................................N.... ..........................N............................N........................ ...........N........N.............N............................................. .............................i.................................................. ...........................................N.................................... ............................... Pos Score Pred ------------------------ 16 0.522 Yes 35 0.317 - 75 0.118 - 91 0.382 - 98 0.233 - 104 0.610 Yes 126 0.400 - 138 0.726 Yes 193 0.199 - 199 0.118 - 244 0.155 - 424 0.206 - 558 0.357 - 568 0.343 - 644 0.571 Yes 700 0.567 Yes 846 0.680 Yes 850 0.223 - 862 0.287 - 937 0.227 - 974 0.408 - 998 0.715 Yes 1008 0.533 Yes 1018 0.319 - 1058 0.346 - 1096 0.226 - 1180 0.112 - 1183 0.289 - 1216 0.119 - 1287 0.204 - 1361 0.595 Yes 1387 0.146 - 1414 0.217 - 1431 0.819 Yes 1492 0.323 - 1591 0.397 - 1632 0.626 Yes 1654 0.245 - 1658 0.529 Yes 1696 0.107 - 1704 0.544 Yes 1728 0.579 Yes 1737 0.756 Yes 1837 0.244 - 1876 0.114 - 1894 0.464 - 1954 0.361 - 1962 0.386 - 2035 0.078 - 2045 0.559 Yes 2059 0.162 - 2092 0.315 - 2139 0.366 - 2230 0.086 - 2325 0.458 - 2445 0.799 Yes 2500 0.344 - 2529 0.535 Yes 2637 0.514 Yes 2646 0.628 Yes 2668 0.299 - 2676 0.673 Yes 2709 0.392 - 2716 0.373 - 2755 0.173 - 2885 0.253 - 2889 0.863 Yes 3097 0.072 - 3118 0.375 - 3124 0.321 - 3136 0.145 - 3156 0.680 Yes 3214 0.131 - 3229 0.302 - 3233 0.425 - 3238 0.172 - 3358 0.552 Yes 3446 0.187 - 3468 0.595 Yes 3481 0.155 - 3487 0.266 - 3490 0.292 - 3496 0.280 - 3508 0.275 - 3555 0.758 Yes 3561 0.610 Yes 3569 0.430 - 3576 0.269 - 3579 0.589 Yes 3582 0.655 Yes 3592 0.199 - 3598 0.459 - 3631 0.091 - 3667 0.096 - 3679 0.181 - 3757 0.267 - 3774 0.657 Yes 3786 0.390 - 3877 0.400 - 3886 0.632 Yes 3934 0.223 - 3937 0.200 - 3970 0.174 - 4002 0.678 Yes 4006 0.071 - 4015 0.176 - 4042 0.172 - 4059 0.439 - 4264 0.185 - 4273 0.129 - 4356 0.691 Yes 4438 0.289 - 4443 0.701 Yes 4447 0.239 - 4564 0.182 - 4579 0.357 - 4597 0.168 - 4618 0.157 - 4627 0.160 - 4650 0.531 Yes 4666 0.203 - 4686 0.480 - 4738 0.105 - 4792 0.076 - 4800 0.510 Yes 4816 0.217 - 4842 0.377 - 4911 0.592 Yes 4950 0.534 Yes 4956 0.716 Yes 4996 0.180 - 5026 0.163 - 5047 0.095 - 5091 0.451 - 5119 0.331 - 5175 0.234 - 5184 0.669 Yes 5203 0.392 - 5296 0.145 - 5303 0.420 - 5313 0.467 - 5322 0.260 - 5333 0.583 Yes 5354 0.477 - 5458 0.442 - 5479 0.313 - 5542 0.357 - 5596 0.182 - 5608 0.412 - 5615 0.613 Yes 5698 0.228 - 5722 0.105 - 5737 0.614 Yes 5803 0.155 - 5820 0.431 - 5830 0.385 - 5851 0.251 - 5863 0.330 - 5881 0.108 - 5902 0.226 - 5992 0.129 - 6045 0.475 - 6049 0.354 - 6183 0.707 Yes 6210 0.413 - 6286 0.449 - 6305 0.198 - 6346 0.383 - 6397 0.195 - 6453 0.335 - 6472 0.457 - 6547 0.293 - 6571 0.241 - 6588 0.489 - 6618 0.660 Yes 6697 0.657 Yes 6715 0.126 - 6881 0.380 - 6922 0.438 - 6992 0.392 - 7087 0.182 - 7156 0.172 - 7195 0.061 - 7208 0.389 - 7228 0.099 - 7240 0.260 - 7294 0.391 - 7370 0.562 Yes 7416 0.596 Yes 7462 0.202 - 7468 0.200 - 7487 0.357 - 7510 0.292 - 7519 0.215 - 7524 0.783 Yes 7527 0.682 Yes 7576 0.148 - 7593 0.414 - 7597 0.172 - 7678 0.375 - 7702 0.193 - 7777 0.560 Yes 7810 0.158 - 7863 0.877 Yes 7924 0.074 - 7930 0.069 - 8005 0.287 - 8029 0.172 - 8080 0.210 - 8115 0.587 Yes 8158 0.153 - 8161 0.468 - 8295 0.706 Yes 8317 0.126 - 8440 0.176 - 8478 0.423 - 8497 0.458 - 8536 0.287 - 8551 0.576 Yes 8622 0.385 - 8680 0.170 - 8687 0.513 Yes 8743 0.457 - 8757 0.642 Yes 8769 0.444 - 8776 0.197 - 8801 0.291 - 8821 0.141 - 8830 0.172 - 8866 0.406 - 8893 0.414 - 8920 0.545 Yes 8930 0.224 - 8936 0.149 - 8953 0.432 - 9004 0.647 Yes 9008 0.263 - 9156 0.446 - 9197 0.496 - 9217 0.135 - 9256 0.263 - 9308 0.374 - 9370 0.424 - 9400 0.113 - 9451 0.306 - 9577 0.297 - 9694 0.545 Yes 9762 0.659 Yes 9787 0.677 Yes 9825 0.501 Yes 9834 0.582 Yes 9901 0.445 - 10000 0.360 - 10035 0.293 - 10056 0.635 Yes 10163 0.706 Yes 10199 0.424 - 10253 0.661 Yes 10290 0.332 - 10315 0.591 Yes 10375 0.506 Yes 10434 0.258 - 10501 0.249 - 10564 0.518 Yes 10710 0.347 - 10783 0.517 Yes 10831 0.056 - 10876 0.186 - 10899 0.448 - 10971 0.286 - 11013 0.632 Yes 11092 0.530 Yes 11170 0.137 - 11194 0.128 - 11199 0.463 - 11316 0.496 - 11322 0.605 Yes 11373 0.618 Yes 11400 0.615 Yes 11428 0.188 - 11442 0.566 Yes 11457 0.564 Yes 11467 0.531 Yes 11542 0.479 - 11565 0.630 Yes 11592 0.841 Yes 11857 0.310 - 11862 0.590 Yes 11868 0.419 - 11896 0.346 - 11962 0.114 - 12067 0.540 Yes 12111 0.454 - 12159 0.773 Yes 12190 0.458 - 12218 0.500 - 12223 0.212 - 12237 0.347 - 12316 0.338 - 12347 0.451 - 12376 0.154 - 12412 0.265 - 12421 0.230 - 12435 0.301 - 12510 0.724 Yes 12604 0.115 -

Explain the output -- Processing locus 2: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCCTTGGAAAAAAATTTGTTTTTTATTTTTTGATCAATGATTCGATTTTTCTTGAAAAGATTTGATTTTGTTGATGAT TTGGTAATCAAATATGAGTAATATGGAGTCTTGATGCAGAAAGATGATTTCTTTTTTGGCTTTTTTTGGGGTGCAGTGAT GAAATTACAAAACCCAGATAAGAAAACCCTAAGAGAAGGCTTTAGTCAGGAATCTAGTGTAGTGGCTTTAGATTCTGGAG TTTTAGCTATGAGTGGTTTGAAATGCGATGGGAAATTCCCGGTCAAGGATGTATTAATGGAAGAAGGTGGTGATAAAGTG AGGAAGATTCAAGTTTCTGGGGGAAATATATCACTTGTTGTTGATTTTAGTGGAGCTAGGACTAGTTCTAACAATTTCTT TGAGTCTAATGCTAGTTGTGTCAATGAGAATCTGGTTAAAGGAAATGGGTACAGAGAAGATGAAACTCAGGAGTTTCTTG TTGGTAATCTTGTGTGGGTGATGACTAAATACAAGAAGTGGTGGCCAGGAGAAGTGGTTGATTTTAAAGCTGATGCAAAG GAGAGTTTTATGGTTAGGTCTATTGGTCAGAGCCATCTTGTTTCGTGGTTCGCTTCTTCGAAGTTGAAACCTTTCAAGGA GAGCTTTGAGCAGGTGTTGAATCAAAGAAACGATAACGGGTTTTTCGATGCGTTACAGAAAGCTATGTCCTTGTTGAGCA ACTCGTTGAAACTTGATATGACTTGTTCTTGCATTGCTGATGGGAATGGAATTGTGTCAGCCCAAAATATAACAACTCGG AAGAACAAGCCTTTGATACTGCGTGAATTCTCAGTAGATCGGTTGGAACCAAAAGAGTTCGTCACTCAGCTGAAGAATAT TGCGAAATGTGTTTTGAATGCTGGGGTACTCGAGTCGACAGTTATGCAGAGTCAGTTATCAGCTTTTTATACTCTCTTTG GGCATAAGCAGATACCAATGGCCCAACTACACGAAAATGAGGGTAGGAAAAGTTTCACTGCGAAGATGAGTGACAGTAAG TTCATTGGTTCTCCATCCATTTGTGCAGGTAATAGCAGGAAGAGGTTTCGGAAAGAATGGTTTAGGAAATTTGTAAGTGA GGTTGATAATGTATCTGCGAGAGACGATCTAGTAAATGTGCCTCCTTCTGATTTGATATCTAAACTCAAGCTGCTTGCTG TGGGCTACAATTGTTCGGAAGAGACTGAAAATATTGGGCTCTTTGAGTGGTTCTTCTCCAAATTCAGAATTTCTGTGTAT CATGATGAAAATGCTTACAAGATGCAGTTGGCTAACATGGCTGGTTTCAAGGATTTGATGTTAGCAACAAATGCAAATCG TGGTACTGTTCAGAAAACCTTAAAGTCAAAGAAAATAGGGAAGTCAAAGATGGAGCCTCTCAATGGTGTTTCAGTTGCTG ATACTGAGCAGAAGACCTTTGAGTTGCAGATATCAAAGAAGTCCAACATCGAATCTCTTAATGGTGTTTCAGTTGCTGAT ACTGAGCAGAAGACCTTTGAGTTGCAGATATTAGAGAAGTCCAACATCGAATCTCTTAATGGTGTTTCAACTCCAAATAT TGACCACGAGGCCTCCAAGTCAAATAATTCAGGGAAGACAAAGATCAATCATATCATTGGTCACTCAAATTTTCCTAGTT CTGTTGCAAAGGTGCAGTTGGCGAAAGATTTTCAAGACAAACTGTTGGTACAAGCTCCAGACAGGAAAGCTATGACGGCA GATACACTCAGCAGACCTGCTGCTATACTTGTACCGGACCTGAACAGCGGAGGCAATGCTTTGGGTACTGCAGAGTTTGA CCATATGCAAAGACCTGAGACTTTAATTCAGCATAATGTCTGCCCACAAGAGGAGAAGACACCAAGATCTACGATACTTA ACTTTCAGGTGACAGCTCATCAGGGAGTCTCAGGAACTCAGTTTGTTTCTAGCCAGCCAACTTCATACAAACACTTCACG AGTGCAGACCTTTTCACTTATTCAGGGAAGAAAAAGAGAGGAAGGAAGAGGAAGAATGCAGAAGAGCTTCCAATAGTTGC ACATGCCAGTGCCACTACTGGGATACCTGACTTAAATGGAACAAATACAGAGCCAACTTTGGTTCTGCCTCAAGTTGAGC CTACTCAGCGCAGAAGACGTCGGAAGAAAGAGGAGTCACCTAATGGATTAACCAGAGGAATCACAATTCTCTTCTTGAAA TTTTCCTCCCAAGTGTCAATGCCTTCAAGGGACGACCTAACCTCGACATTCTCTGCGTTTGGCCCGTTGGATTCTTCAGA AACACACGTTTCAGAAGAATTTAGTGGTGCACAGGTCGCCTTTGTGAGTAGTGCAGACGCGATAGAAGCAGTTAAAAGCT TAGAGAAAGCTAACCCATTTGGTGAAACCTTAGTGAATTTCCGGTTACAGCAGAAACTGATAACCGTTCAAAGGAACATT GCCCCACGAATGCCGGTAATCTCACATGTTAGTCCAGTTCCGAAACCGAACAATATACCAACAAGTATGGATGCAATGAG GCAGAATCTGTTGATGATGACAGCAATGCTGGAGAAATCCGGGGATAGTTTATCAAGAGAGACAAAGGCAAAACTGAAAA GCGAGATCACGGGGCTTCTGGAAAAGGATGGTGTCAAGTTATTGAATACATGGCTCGAAGGGGAAAGGTCAATTACCTTT TGTAGATTTCTTTCGCAAAACACTGCTAAGCTAAAGTTGGACGAGATTCCAAATGCTGAAACGTGGCCCTTTTTGGTTAA ACTTCTCCTTCAATGTGTCTCAATGGAGGTGTCAGGTAGTAAGCGACGGATGCCCAAGCCAACTTTTGCAAAGACACTTC GCGTTGTCGTTCAGCGAACAGAAGAAACTAAATTTCCTGGTGTACAATTTCCTTTGCTATCTATGGCTAAAACCCTTTTT ACTCATGTACATGATATCTTGAGCAACACACCAAGCTTTCAGTCAGAGTATGGTACCATACTCCGCCATCTTTTGGAAAT TAAGGAGTACCGTTTTCAAATGAGGAAGCGTACATATTCCAGTTTGGTGCTGCTATACATGGAAAGGGCTGAGACAGGGT TTTGCGAGAAAAACAGTGGTCAACATAGCCAGAAGGAGGAAGCTTTTCGCTATATTCTTACGCTTCAGTCACTTCTAGAG AACTCCCCTGGTGATTTTCCTGATGACCTTCGAGAAGAAATTGTGAACGGCCTCATTCACATCTTCTCTTCTGTAAGAGA TGAGGGGAAGCTTTCACGCAAGCTTATTGAATGTGTAAATACGTTCTTGCTGAAGGATGGTCCTAATTTAGGCTCTTTGT CATTGGAAATTCATAATGCGGTTGAACAATTTGTGTTCCGTTGCTGGTTAACAACCCATGATAAAAACCTCAAGGAGATA CTTGTTTCTTATGGAAGGCTACAGTTAAATTTGACAAGGGATTCTAGTGAATCTAGTTCTCTAGTGGAACAACTTTTGGA CGTGGTTACTCGTGAATTGGATCTTGGTAGTTCATCAAGTTCTGCATCATGGGGTGATACAACAAAGGATGAAAAGTTAG GGGCACTGAGCAGCTATCAGAATAGCTTGGTTGAGCTGGCTGCACATGTGTTCTACCGGGCATGTGTCAATACATCTAGA CCATCACTGTCAGAGAAACGAGCTCGTAGGCAACATATTGCGATGCGTATGGTGGATGCACTAACTGAAGGAAAATGGCT ATGGTGTGCTGCTTTTGGTTGTTTAGTTCGGAATTATTGTGCTCGCATCAATATGGATCTCCTTATTTACTGGTTTGAAG CCATCTGCACAAACTTTCAGAGACTTCTGGAGGATGCCAGTATGAGACGTTCTTATGATGGTCTACTATGGACTTTGAGG AGTTTGCAAGGGCTCTCCTCTGGTTTGTCACTTCCAGATATTACTATGGACATATCGAAGTCATCTGCTTCTTCAAGCGA GCTAGATCGTGGTTGGCAATCGATTTGGAGTTCTTTAATCCACGGGCTAGCAACATTCAGCAGCATGAGTGTAATTGTTG ATGCTGTCTTGGTACTCCTTGGATCAATTATTTCAAGTAACCACATTACCGTTAAAATTCTGCCTCAAGAAGTTTGGGAT CATCAATTATTCAGACACATACCCTCTGAGCCGGCCTTGTACTTCATTGCATGTTACTTTTCACGTATGGGTTGCCAGGG AAACCTGCAAGATGATTTACATCTTCGACGAAATCTTCTTAGAGCAGTTTGTGCCCCTCTAAGTTGGAAGGTTCGTTTGA CTCTAGATGAGAGAATGGTCCAGCTTCTACCAGCAGCTGCTTTTTCCCTATGTGCTGGTTTCAAGGTTTCACTTCCATTG CCTAAGGAACATCTTCCAACACCATCTCAATGGGATGTCTGTGAACAGATAGATGATGTTGATCGAGAAAGAAATTTTGG ACTCTTTGAATGCTCTGTGGAGGCTCTAACAAGAATTTGCTCAAATTCTAGCAAGATCTCTGGCTGCCAAGTTCCTGACG TAGTACAACTACCTCTGGTGTTAAGAGATCCATTGCTTCATGACATGGATATCTATTTTCTTAGCATTATACCGGAGGTC AAGGAGAAAGGACCATTGTCTGACATCTTTATGGGATGTGCTCTGTTGTGTCATTTTATGCATGGTTCTTACATCACAAG GAAGGGTAAAGGAAGCAGTTCGTTCTTCTTAAAGGCATGCCAGTATTTGTTAGAAGGCCTGGATCATGCTGTTGAATCAG TTTCAAAGAGCCTCAATGATCTTCAAAGGCGTGGTTCCTTAGGGTTTGGCTCAGATTTTAATGAGAAAGGTTCTATAATA GTTTCATTGAGGTCCTTTACTCAATCTCCTGTCTTCAGCAACAGAAGAGACCAAAATCTCCTTGGCGCTAGTTATGATTT TGTAATTCATTCCTTAGAGAACCTTTTGCGGTCTTTTGCTAAAGTCTATGAAGAATATACTGAGCACGCATGGAATACAC ATTCAGACACTGTACCTTCTAAATCCTTAGCACCTGATTCCCCAGAAGTTGGCAGGATTGTGGATATGGATTTAGATTTA GCTGAAGACACTAAAGAGAGGGATATTATAGCTGCTGGGGGAAAAGCTGTACCTGGCCTGCCCGTTTCTATGGGTAACTG GAAGTTGGGCATGGTATCGCTTATCTCATGTTTTTCTCCGGTACTTCAGTTTCCTACATGGGATGTTCTATATAATCTAT TGGAGAAGGAATCTGATCCTAAGGTACTGGAAAATATACTGTATCATCTATGCAAGCTTTCATGCTTGACTTCTATTCCG AAGGTTGATGATTTGGTTATTTTCTTGGATGGCATGCTTAGCACACAAGTAAAGATGAAGCGTAACTGTCTCAATATAGT CACTGCTCTGCATGTTTTACTTCATACTTTATCATCCTCGAGGAGGGATTCCTCTGGAGTCGAAAAAAATTGTGGTTTGT CTTTGAAAGAAGCAGAAAGTTTTCAGGTCTTTGTCCAACTTGGCGCAATGGTGAATAAAGTTTCTGAATTTGGTCTCTTG GGATGGTTTGGGCGTGTCAAGCTGATCAACTGTATATGTGATCTTGTCTTACTTAATCCTCAGACTGGTCAGACAATGAT TGAGCGACTTCTGTTGATGCTAAGTGACTCTGATTATCGAGTGAGGTTTGTCTTGGCAAGACAAATTGGGATTTTATTCC AGACGTGGGATGGCCATGAGGCATTATTCCAAGATATTTGCTCCAGCTTTGGTATTAAATTGGTAACCTCCTCAAAGGAG AAACTAGTTACCGCAAAAGATGTTTTGGCTGTTGGTCCTCAGCCTCGCCAAAAAATGGAGACTGTCATCATTACTCTTAT GCACCTCGCTTATCACAGTGAGAACATAGAGTTGCAGGCTGTATTTATGATGTGCGCTGTTTCAGCTAAAGATCCCTGTC AGAGGGAATTAATCATTGCCGCACTTGACAATTTATCAGCACAACTCCATTACCCATCTAGGTTCAAGTACCTGGAAGAA CTCTTGGGCCCAATCCTTTTTCACTGGATTGCATCTGGTGTCAGTTTAGCTGGCCTTATTGAGACAAGTCAGCTTTTCAT TCCAAACGCTGAACCGAAATATTTCATCCACTTTTGTTCCCACTGGCTGCTTCCAGCTCTTCTATTGCATGAAGATCATA CTAACCTCGACTGGGTAGCTAAGATGGCTGGCCAACCAGTGGTTGTTTTGGTCAAAGAAAATTTTGTGCCAATATTTTCG ATATGTATGGGATTGCACTGTAGTAAAACGTCAGAATGTGATAAAGGTGCAATGGTGCTTCAGAATTCAATATTGTATGT AGGTGAGACCAGCGAGAATGAGAGGGACAAGCTGATTAAACAGAATATGGTGTCTATTGTTAGTTTTATTTTATCATGCG CTTCGTCTTCACCGGAACCTCCAGTTCCTACTTTTTCTCGTGACACTATTTCACTTGCAGTTCAAACAGTTGTGGATGGT TTTCTGGAAAACACTGATTATCCTAAGAATGCGGCCATCACTGACAGGATAAACATTTTTCGCCCGGATAGAGTGTTCAT GTTCATTACAGAAATGCACTATAGAATGTCAGCTGCATGCCATCATCGGCATACGCGTCATCATTTGGCCGCTCTTGAAG AGCTTACAATTCTTCTTGGTCATAGAGCGTTAGTTCCAAGTTCCTTAAACTACATATTCAATCTTGTTGGACAATTTATC GGCTATCCCTCACTACAAGACCAGTGTTGTAGTATAGCGTCCTGTTTGCTGGATTTGTTCAAAAGCAATCCTGCCAAAGA AATTGTTAGTGTACTAGGTGACCAACTCCAATTTTTGGTTTCAAAGCTGGTTACATGCTGCATTGATGCTGAAGCAGATA CCAAAATTTCTGGTGCGAAGTCATCTCAACTGGTTAATTTGCTACACAAGCTAGTTGTTAGTTCAGACTCTTCTCTTAAT GAAGATATCAGAGACTTGGAACCACTCCCGGATTTAAAATATTTCCAAGTCATTCGTGAATCACACATTAGGATCTGCGA AGCATATTCTCCGAGGAATCATCTGTTGAAGGTTGAACACTCTACATTTTTAATTTACATTTTTCTTGAAATTTTATCAC TATCCAACTTTTTGTTTCTTTCCTGTTCCACTATCCAACAGTGTTCTAGAAGATCTAATTATCTTCCACCGAGATTCCTT TCCAGGAGTCTCCAAGCACTGCATAACAAGCTCATAGCTTCTGAAGTTTCTCAAGAAGATACAAATGGAGAAACTGCAGA AACATTTTGGCAGTCTGACGATGAAATTGTAAATGCTGTTTGGACTCTTGTCCGAGTGTCTGCCTCTGATGAAGCCGATA GTATGAGACTTTTGGTGTCTGATTTTCTTTCCAGGATTGGTATTAGGGACCCCCACACTGTTGTTTTCCATCTTCCTGGG AACTTAGTCTCCATGCATGGTCTTCAAGGTTTTGGCCATAATACTGGATCAAAAGTCAGATCTTTGACTGAGAACGGCAT ATCCGATGAAACCCTTATCACACTGCTAAATTTTCTGAAGAAGTATCTTTTGGATGACTCCGTAAAGATTATTGATGTAA CATCACAAACCCTTCGGGGAATTCTTTCAACCGAAAGGGGCCAGCAAGCATTATCATCTTTTGATTCATGTGAGAGAGCT TTGATTGAGGTGCATGGTAGGGGCGTGAACCTTGACATTGTGGAAAAGATCTTATTGGATAGCCAAAAGCAGTTCAAAGC CGAGAAATTTTCACTGGAGACGCCCGAAGTGTGGTCCACAGATAATAAAAACTTTGATAGATGGATTTGTCAGCTTGTGT ACTGTATGATCGCTTTATGTGAGGATGTCCCAATAAGGTTATGCCAGAACATAGCATTGCTGAAAGCTGAAATCTCTGAG CTTCTATTCCCTAGTGTTGTTGTTAGTCTTGCGGGACGGATAGGGATGGATATCAATTTACATGACTTAATTACGTCACA GGTGAAAGAGCATATCTTCACTGATTCAAACAAGCTAACAAAATCGAAGCAAGTTATGTTAAATACTCTGAATGAACTGC GGATGTGTTATGTCCTCGAAAGATCGATTTTTTCTGGGCAAACAAAGAGAGAAAAGAATTCAAGATCTTGTTCCACAGCT GCTAAGATTAGAGATGTGGAAAGTGGATCAAATGGAATGGCAGCGTCCATAACTACTAATTGGGAGAAGGTTTATTGGCT TTCCATTGATTATCTTGTTGTTGCCGGATCAGCAGTAGTTTGTGGTGCATATTTGACTGCTTCCATGTATGTGGAGTATT GGTGTGAGGAGAAATTTGGCAATCTAAGCCTTGGAGATCCTGATTTTTCTTATCATGATAAGTTACCGGATCATGTTGAG ATACTTGTGTCTGCAATAACAAGAATAAACGAGCCTGACAGCTTATATGGGGTTATACATTCAAATAAGTTGTCTGCTCA AATAATCACATTCGAGCATGAGGGAAACTGGACTAGGGCACTCGAGTATTATGACTTGCAAGCACGTTCACAGAAAATGG TGGTGCCAAGTAGCCTTTCTGAAAATCTAGAGGTGGAACAATTTCAGCCAACAACTAGTGCACGGCATTCGGTTTTTGGT GAAGGGGAGGTCCAGAGACAACCGTTCAAAGGACTCATTAGGTCGTTGCAGCAGACAGGCTGTATGCATGTCCTGGATTT GTATTGCCGAGGCTTAACTTCCCGAGAAGGATGCTTTCAGTATGATCCCGAGTTCATCGAATTGCAGTATGAGGCTGCAT GGCGAGCAGGAAAATGGGATTTTTCATTACTTTATCCGCAAACACATTGCCAACCTCTGCAACATGCAAAGAACAATAAT TATCATGAAAGTTTGCACTGTTGTTTGAGAGCATTGCAAGAAGGGGATTACGATGGGTTTTATGGAAAACTGAAGGATAC TAAGAAGGAGCTTGTGTTGTCCATTTCCCGTGCAAGCGAAGAAAGCACTGAATTTATATACTCAACAGTAGTGAAACTTC AGATTCTTCATCATCTTGGACTAGTTTGGGATCTTCGCTGGACGACATCTTCGCATCAAAGTGTGCATGGCTATCTAGTC AAACAAATGGCATGCGTAGACCCCGTCATTCCAACAATGGATCAGTTGTCTTGGCTGAATAAGGATTGGAACTCTATTAT TACGCAAACTCAGTTGCACATGACTTTGTTGGAGCCATTTATCGCATTTAGGCGAGTTCTTCTCCAAATCTTGGGATGTG AGAAATGTACCATGCAACATCTTTTGCAGTCTGCTTCATTACTTCGCAAGGGAACAAGGTTTTCTCATGCAGCTGCGTCT CTGCATGAGTTCAAGTTTCTTTGTGCAAGAAGTAATGGACAACAACCCGTTCCAGACTGGCTTGGAAAGCTTGAAGAGGC GAAGCTATTACATGCCCAAGGGCGGCATGAAGTTTCCATTAGTCTTGCAAACTACATTTTACATAATTATCAATTAAAGG AAGAGGCTTCAGATATATATCGTGTCATTGGAAAGTGGCTAGCAGAAACCAGATCTAGCAATTCTAGAACTATTTTAGAG AAGTATCTCAGGCCTGCAGTTTCGCTTGCTGAAGAACAGAGTTCCAAGATCTGCAAAAGATTAGTAGATAGACAGAGCCA AACTTGGTTTCATCTGGCTCATTATGCAGATGCTCTATTTAAGAGTTATGAGGAAAGACTCTCCTCCAGTGAATGGCAAG CTGCACTGCGGCTACGAAAACACAAGACGAAAGAGTTGGAGGTGTTTATTAAACGGTTTAAGAGTTCAAAGAAGGCAACA ATCTGACTATTCACTGAAGATCCAAGATCTGCAGAAGCAACTAACAATGGATAAAGAAGAAGCAGAAAAACTGCAGGTTT TTTTTATTCTGCCTTGATAATCTGG ......................................N....................................i.... .............N........N..........N.........N..................................N. ................................................................................ ........i.............N....N....................N.......i....................... ................................................................................ ........N..............N....................N..............N.................... ....................N...................................................N....... .........N...................................................................... ...............................................N................i............... .................N.....................N.....N.................................. ................................................................................ ......i..........N.........................i.................................... .................i..................i............................N.............. ........................................................i....................... ........N..........................N............................................ ................................................................................ .................................................i............N................. ............................................................N................... ..........................................................N..................... ................................................................................ .......................................................................i........ .......................................................N........................ ....N..............................N............................................ ................................................................................ .......................................................N........................ ..N................................N............................................ ..........................................N..................................... ..................i............................................................. ................................................................................ ................................................................................ .........N...............N........................................i...N....N.... .............N..N........N...................................................... ...........................N.....................i.............................. ....................................................N........................... ............N.........i..........................N.............................. ..............................................................i................. ....N.....N......................................N.............................. ...................i......................................N..................... ................................................................................ ......................N........................................................N ..............................N.........................N....................... ...............N.........................................N...................... ..........N..................................................................... ................................................N...................N........... .............................................N...............i.................. ..........................................N.....N......N..................N..... i...................................................i........................... .................................N.......N............N..N.........i............ .............................................i.................................. ................................................................N............... N............................................................................... ..................................................N...............N............. ...........N.................................................................... ......N.......i..................................N.............................. .............................N....i.................N..i........................ .........N...................................................................... .......................................N....N................................... ..............................N....N.....................N...N.................. ....................................N............................N.............. ...............N............................................N................... .........................................................................N...... ...............................................N.....................i.......... .................................................................i.............. .....................................................................N.......... ..........N................N.............................N....N................. .................................................N...........N.................. .......N....................N....N....................N......................... ...........N.................................................................... ...............................................i................................ ..i................................N.......................................N.... ................N............................................................... .........N.....N................................................................ ...................N..................................i.......................N. ..............................................i..i.............................. ................................................................................ ................................................................................ ....................................................................N........... .......................i........................................................ ..N...N............................i...............i........................N... .................N............................i............................N.... ...........................................................................N.... ............................N.................................................N. .............i...........N..........N........................................... ................................................................................ ................................................................................ ......................................................N..........N.............. ..............................................................................N. ................................................................................ ................................................................................ ................................................................................ ................................................................N............... ....................N...........N...................................N........... ..N............................................................................. ............N...N............................................................... .....N...............................................N....................N..... ...................................................................N............ .............N.................................................................. ............................................................i................... .....N..........N.......N...............i....................................... .............................................N...............N.................. .......................................................i...............N........ ..N......i...................................................................... .............i.................N....i........................................... ................................................................N...N........... ......................................................N.................N....... ..............................................N................................. .................N................................N.........................i... ................................................................................ ...............................................................N...i............ ..............................N..........i..........................N.........N. .............N.................................................N................ ....N...............................................N........N.................. ................................................................................ ..................................................................N............. ......i....i........................N........................................... ...................N.......................................................N.... ....N......N......................................................N............. ....N.............................N............................................. ...........N..............N..................................................... ................................................................................ ................................................................................ .......................N.....N.................N........................N....... ................................................................................ ..............................................N................................. ......................... Pos Score Pred ------------------------ 39 0.106 - 76 0.501 Yes 94 0.225 - 103 0.291 - 114 0.361 - 124 0.237 - 159 0.355 - 249 0.583 Yes 263 0.269 - 268 0.159 - 289 0.139 - 297 0.790 Yes 409 0.099 - 424 0.116 - 445 0.289 - 460 0.101 - 501 0.296 - 553 0.159 - 570 0.360 - 688 0.192 - 705 0.543 Yes 738 0.401 - 760 0.427 - 766 0.263 - 887 0.608 Yes 898 0.337 - 924 0.540 Yes 978 0.604 Yes 997 0.574 Yes 1026 0.418 - 1097 0.612 Yes 1129 0.236 - 1156 0.133 - 1282 0.165 - 1285 0.198 - 1291 0.229 - 1302 0.558 Yes 1317 0.586 Yes 1338 0.278 - 1351 0.099 - 1410 0.721 Yes 1423 0.090 - 1501 0.121 - 1579 0.279 - 1752 0.741 Yes 1816 0.312 - 1845 0.492 - 1876 0.322 - 2056 0.323 - 2083 0.201 - 2116 0.268 - 2203 0.234 - 2259 0.614 Yes 2490 0.488 - 2506 0.204 - 2547 0.548 Yes 2551 0.371 - 2556 0.451 - 2574 0.261 - 2577 0.328 - 2586 0.325 - 2668 0.420 - 2690 0.508 Yes 2773 0.148 - 2813 0.448 - 2823 0.610 Yes 2850 0.348 - 2943 0.550 Yes 2965 0.127 - 2971 0.159 - 3010 0.402 - 3060 0.646 Yes 3099 0.426 - 3223 0.166 - 3280 0.159 - 3311 0.306 - 3337 0.413 - 3376 0.405 - 3418 0.241 - 3451 0.150 - 3569 0.387 - 3589 0.194 - 3646 0.209 - 3662 0.608 Yes 3723 0.356 - 3729 0.420 - 3736 0.215 - 3755 0.497 - 3761 0.528 Yes 3813 0.525 Yes 3874 0.187 - 3882 0.291 - 3895 0.315 - 3898 0.159 - 3908 0.668 Yes 3966 0.510 Yes 4065 0.464 - 4081 0.380 - 4211 0.205 - 4227 0.400 - 4252 0.492 - 4327 0.175 - 4335 0.623 Yes 4370 0.362 - 4430 0.273 - 4435 0.526 Yes 4453 0.224 - 4456 0.542 Yes 4490 0.362 - 4600 0.186 - 4605 0.210 - 4671 0.116 - 4676 0.243 - 4698 0.156 - 4702 0.123 - 4757 0.124 - 4786 0.292 - 4816 0.223 - 4861 0.208 - 4954 0.209 - 5008 0.217 - 5030 0.675 Yes 5106 0.740 Yes 5190 0.250 - 5211 0.467 - 5228 0.359 - 5258 0.323 - 5263 0.153 - 5330 0.371 - 5342 0.284 - 5368 0.155 - 5389 0.167 - 5394 0.124 - 5415 0.440 - 5452 0.135 - 5568 0.586 Yes 5603 0.619 Yes 5636 0.302 - 5676 0.262 - 5697 0.106 - 5770 0.214 - 5776 0.209 - 5860 0.368 - 5895 0.825 Yes 5919 0.254 - 5967 0.612 Yes 5970 0.716 Yes 6229 0.180 - 6264 0.707 Yes 6323 0.221 - 6327 0.472 - 6356 0.599 Yes 6372 0.552 Yes 6397 0.130 - 6418 0.239 - 6447 0.633 Yes 6476 0.193 - 6556 0.216 - 6589 0.391 - 6639 0.402 - 6654 0.527 Yes 6666 0.468 - 6677 0.215 - 6935 0.223 - 6946 0.124 - 7039 0.342 - 7345 0.290 - 7381 0.081 - 7393 0.194 - 7429 0.077 - 7443 0.445 - 7533 0.448 - 7537 0.207 - 7606 0.066 - 7654 0.463 - 7675 0.261 - 7748 0.238 - 7774 0.324 - 7901 0.710 Yes 7926 0.131 - 7937 0.360 - 7945 0.313 - 7961 0.516 Yes 8046 0.333 - 8062 0.181 - 8136 0.596 Yes 8152 0.356 - 8163 0.434 - 8170 0.722 Yes 8254 0.533 Yes 8272 0.125 - 8277 0.760 Yes 8385 0.430 - 8389 0.088 - 8455 0.219 - 8473 0.111 - 8527 0.391 - 8578 0.216 - 8611 0.382 - 8637 0.769 Yes 8784 0.307 - 8788 0.779 Yes 8831 0.196 - 8842 0.692 Yes 8869 0.421 - 8879 0.271 - 8894 0.389 - 8944 0.376 - 8965 0.208 - 9013 0.442 - 9022 0.200 - 9187 0.304 - 9207 0.607 Yes 9212 0.502 Yes 9237 0.494 - 9300 0.225 - 9356 0.469 - 9365 0.259 - 9372 0.440 - 9427 0.225 - 9445 0.323 - 9475 0.429 - 9532 0.240 - 9547 0.308 - 9784 0.273 - 9790 0.203 - 9808 0.231 - 9833 0.437 - 9967 0.083 -

Explain the output -- Processing locus 3: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGCGATTTCGAGCGGTGTAGTACGGTCCGGAGCGATTGAAAACATTTTCTGTAATTGGATATAACGTTAGCAACTGCAAT CACCCGCAAATGCAGCGTTAGCGGCGGGTAGCGGAAAAACCAGTTAAACCCTTAAATGATATTTTCCCAAATATGTTTTT TTTTAATTTTTTTTTTAACATTTTATTTTCTTTTAAAAATTGGGAGGGAAGGGGAAGGGGACTGGAGCGAGAGAGCTTCT GCGATTGTGAAATGGCGAAGGACGACAATAATCTGTCGAGCTTGGTTCACGAACTTCGTGAACGGGTGGCCGCATCCGCC TCCACTCCGGCTAACAACCTCCGTCACTCTTCCGGAGACGAAGATGCTCTAGAGATTCGATTTCGTGCTGTCATCCCTAA TCTATTGAATACCTACGTTGTTCCTTCTCTCGGCAACGGGAGGGAAGTTACAGCTGTTCTGAAGCTTGTTGGTCACACAG CAAGGAACATTCCAGGAGTCTTCTATCACGGAACCCCTTCTGCTATCCTCCCTGTGATTGCTCGTATTATTCCCTTCTTT GCTGAACCAGAATTTGTTCCTGGGCATGGAGTATTACTTGAAACTGTTGGCTCTCTCTTAATGTTGCTGCGTTCCAACTC AAGGAAGGCATACCGGATCTTCTTCCATGACGCTTTGCAGGCAATTCAAGATATGCAACCTATTGCATCACTGCACTCAA TTGAACCGGAAGTTTGTGAATCTCACATTCCATTTAGGTGTTTCTGTATGTCCTTTTCTGGAATTGGGGGTGATCTGCCA GATGCTAATAAGCCCAGAGATGGTGATGGTCTTGTGTTGAACTTATTGGGAGCCAACCGATGGCAACCTTTTGCTACTTG TATTCTTAAACTCATCTGTAAATGCCTAACTGAGGGGACACTCTACGTCCAGGGTCTCATCCATACATCATTTTTTAAGG CGGCTTGTTCTCTAGTATGCTGTGGTGGCGCAGATGTGCAGATGGCATGCTTTGAATTTGCTACTCTTGTCGGATCAATC CTTACATTCAACATCCTCCCGCATGTGGCCTTAATACAGTCAATCATCCTCCTTTTAAGTGCAGATGAAGGACTTCCTGT GTACAGGAATACGATTTATGATTCTACCATTGGACGTTTCCTTACGGCAGTATATTCCAGCTGTTCTGATGCCGCTGTCA AACTAACTGCAGAAAGTTTAGTGTTGGTTCTCTCTCATGCTTTACAGAGAACAAAAAGTGAGGAGCTTAAGGCTTCCCTC TGCAGTGCATATGTGCGGATTGTGAAATCTTGTCCTCCTTGTATATGGAAGATACACTGCCTTCTAGAGTTGCTTCATCT TCCAGAACCTTGTTTTCAGTTGATAGAATGTTTTAAAGCAGTTCTTATAGTTCTTGGACCTGGGTGTGTTCGAGTTGAGA CAACCAAATGTGGTAGTCATACATCAGCAACAAGTGATAGACCTGTCCAGGGAATTAACGCTGGAAAAAAAAGGCATATT GAGGATGAAAGTACATACAAAAGAAAGCGCCAGAAGGTTGGTGATGATATTCGACGAGGGGTTTACTTTGCTCCTGAATT TGCCGATGAGACTGATGGAAAAGATGCTGCTAGTCTGCGTGAGATGCTTATTTCAACTGTAGAATCTTTAAAGCCTCCAC CTGCTGGACCTAGTCTGTCACAGACTGAAAGTTCAATAGTGGCACTTAGCATGCTCACCAATGCATTTTGTTTCTGTCCC TGGACTGACATGACTCATCGATTGTTTAACCAGATGTATGCCTGGATTCCCTGGATAGCTGGGCAGGTTGAGGAAACAAA TCCTATCATGTTCGATATTTCCATTTACTTGGAAGGAATTCACAATCTGCTGCTCGTTGGTGTGGATCCACAGTATGAAT ATACAAGTAAAGGGAATGATCTGGTTGCCATACAATTCTTGCTGAAGCTTCCTTGGACGCACTACATGCTGTTTAAGACG CCTAGCTCTCTAGTAAAGTCTAAGTGTTTATCTGTAGGGATATGGACAAAGCTTGGTTTACAAGACGGGAGTGATTTTGA TATCTTCAGTTGGTCTCTTTCTGATGATTTTGAACAAGTGCAAGCTGTCGCTGCTATCTCTATGCCACTCAAAGTTCTTT TCTCTGGTCTTGGTGCCCTTCTTCATATGTTCCCCAAGCTGGAGCATTTGTTGGAAGAAAAGGAATTGATGATTAAGAAG GCCATTCCTCAGTCACTTGGCTTCTTATCTTGTCTTTATGGATCAAGTACTACTGACTCAGAGAAAACTGCATGCCATTT ACTCCTACATGAAGATTTAAAGAAAGATGAGACTCTGAATTCTCTACTTCAAGGGTTCCGATGTTCAAAGTGTGATAAAT TTATCGAACGCGAAGATGAAAAGCATTTCAGAATCATAGAGACTCCAGAGATGGTGAAATTGAAGATGGATCATCATCGT GATTACTTTAACCTTCAATCTTTATATTTTAATCTTCTCTATGATGAGTCTTCTGAAGAGACTCAGCTAGCCTGCGTAGA AGTAATAAGGAGAATTCTTGGCCACACATCCCCAGATATTCTGGTAAGAACAAGGTCTCAATGGATCAGGTGCCTTCAAT ATCTATTAGTTCATGTAAATACAGACGTAAGGGAAGCATTCTGTGCTCAGATTGGTATCTTCGTACAGCATCCTATTGTG AGCTGTTTGTTTCTTAGTGAAGATGCTACGGAGAAAAGTTGCGAAAGAAATTTTTTTAATTTGATTGAGCATTCTCTAGC AGCAGCTAAGGATCTTCTTGTTATCCAGACTCTATTGGAAACGACAGCTGAAGTTATGGTAGCTGTTGATGTTACCAGTG AGCTTTTCTTGATTTGTCTTTTCCTGCTGATTGATCAGCTTGATCATCCAAACTTGATCGTCCGAATAAATGCGTCAAAG TTAATAAATCGGTCATGCTACATCCACGTGAAAGGGGGATTTGCAACTCTACTTTCCACAGCCTCTCATATTCAAAATGA GTTGTTTGATAATCTATCTGTCAGGCTGACCAGCCGTCCAAATGTGGTAAGAGAATTTGCAGAAGCTGTTTTGGGTGTTG AAACTGAAGAACTTGTGAGGAAAATGGTCCCTGCTGTTCTTCCTAAGCTCTTGGTGTATTGGCAGGAAAATGCTCAAGCA GCCAATACCTTGAACGAACTGGCCAAGTTAATAGACACAGATGTGGTACCTCTAATAGTAAATTGGCTACCCAGAGTTCT TGCATTTGCTCTGAATCAAGAAGAGGACAAGAATTTGCTCTCAGTTTTGCAATTGTATCACTCACAGATTGGTTCTGACA ACCAAGAAATTTTTGCTGCTGCATTACCTGCACTCTTAGATGAGCTTGTATGCTTTGTGGACATTGCTGATACACCTGAG ACAGATAGACGGCTACAGAGACTGCCTGATGCAATAAAGAAGATCTCAAAAGTTTTAACAAATGCCGAGGATCTTCCTGG ATTCTTACAAAACCATTTTGTTGGACTCCTTAACAGTATTGACAGAAAAATGCTTCATGCAGATGACATTTTTCTTCAAA AACAAGCACTGAAGCGTATTAAACTGCTCATTGAGATGATGGGCCATTATCTCAGCACATATGTTCCTAAGCTTATGGTT CTCCTTATGCATGCCATTGAAAAAGATGCACTTCAGAGCGAGGGTCTCTTGGTCTTGCATTTTTTTACAAGAAAGTTGGC TGATGTATCGCCATCTAGCATAAAGTATGTGATTTCTCAGATTTTTGCGGCACTCATCCCCTTCTTAGAGAAAGAGAAGG AAGGTCCCCATGTATATTTAGATGAAGTGGTGAAAATTCTTGAAGAACTTGTTTTGAAGAATAGAGATATAGTAAAAGAG CATATTTGTGAGTTTCCCCTCTTACCTAGCATACCATCTTTAGGAGAATTAAACAATGCTATTCAAGAAGCACGTGGATT AATGAGCCTGAAGGATCAGTTGCGGGATATTGTAAATGGTATGAAACACGAGAACCTGAATGTTAGATACATGGTGGCAT GTGAGCTAAGCAAATTGCTGTATAACAGAAATGAAGATGTTGCTGCACTGATTGCCGGTGAACTTGTCTCAGACATGGAG ATCTTGAGCTCTTTAATCACATATTTACTACAGGGGTGTGCAGAAGAATCTCGAACTACAGTGGGCCAGAGGTTGAAGTT AGTCTGTGCAGATTGTCTTGGAGCAATAGGTGCTATTGACCCTGCCAAAGTGAGAGTTGCTTCATGCAGTCGTTTTAAAA TCCAATGCTCAGATGATGATCTTATCTTTGAGCTCATTCACAAACACTTGGCAAGAGCTTTTAGAGCTGCACAGGATACA ATAATACAAGACTCAGCTGCTCTTGCCATACAGGAACTACTAAAGATTGCTGGTTGTGAGCCATCATTAGCTGGGAATGT TGTTGTGTTGACCCCCCAGGAGCATGTACAAGTCAATGTATCTGGTTCTAGAAGATGTGGAGGTAACAATGAAGTGAAGG ATAGGGGACAAAAACTATGGGATCGGTTCTCAAACTATGTTAAAGAACTAATAGCTCCCTGCTTGACTTCAAGGTTTCAG CTTCCAAATGTGTCTGACCCTGGATCAGCTGGACCAATTTATCGGCCTTCTATGTCATTCAGAAGGTGGCTATCCTACTG GATAAGGAAATTGACAGCATTTGCAACTGGATCCCGTGTAAGCATATTTGCTGCTTGCCGAGGCATAGTGCGTCACGATA TGCAGACAGCTACTTATCTCTTACCATATTTAGTTCTGGATGTTGTTTGCCATGGGACTGAGGCAGCACGGCTTAGCATT TCTGAAGAGATCCTTTCTGTTCTTGATGCTGCTGCATCAGAAAATAGTGGAGTGACAATAAACAGTTTTGGTGTTGGTCA GAGTGAAGTTTGTGTTCAAGCTGTTTTCACGCTTCTTGATAACCTTGGGCAATGGGTTGACGATGTGAAGCAGGGAGTAG CACTCTCATCGTCTCTGCAGTCATCAGGTGGTAGGCAAGTAGCACCCAAATCGAAAGACCAGGTTTCAAACTCAACAACA GAACAGGATCATCTTCTTGTACAATGTAAATATGTGTTGGAACTTTTGCTAGCTATTCCCAAGGTGACCCTTGCTAGGGC ATCTTTCAGGTGTCAAGCTTATGCCAGGTCATTGATGTACCTTGAATCCCATGTACGTGGAAAGTCAGGCTCCTTAAATC CCGCAGCTGAGAAGACCGGCATCTTTGAAAATGCTGATGTTTCTTCTCTGATGGGAATATACAGCTGCCTTGACGAGCCA GACGGTCTTTCGGGCTTTGCAAGTTTAAGCAAATCATTAAATTTGCAAGACCAGTTGTTGATAAACAAGAAATCTGGTAA CTGGGCAGACGTTTTTACTGCATGTGAACAAGCCCTTCAGATGGAACCAACATCAGTTCAACGACATTCAGATGTTCTTA ATTGTTTACTTAACATGTGCCACCATCAGACAATGGTCACTCATGTTGACGGACTAATTTCCAGAGTACCTGAGTACAAG AAAACGTGGTGCACACAAGGTGTACAAGCTGCATGGAGACTCGGAAAGTGGGACTTGATGGATGAGTATCTTGATGGAGC TGATGCAGAAGGTTTACTATTCAGTAGCTCAGACAGCAATGCCTCATTCGATAGAGATGTAGCAAAGATTCTCCATGCAA TGATGAAGAAGGATCAATACTCTGTAGCTGAGGGAATAGCTATTTCCAAACAAGCTCTCATTGCTCCTCTAGCTGCAGCA GGCATGGACTCCTACACACGAGCTTATCCTTTTGTTGTCAAACTTCACTTGCTAAGAGAGCTAGAGGACTTCCAGGCGGT TCTTAATGGGGATTCATATTTGGAGAAATCATTTAGTACAAGTGATCAGGTGTTTTCGAAAGCAGTAGATAACTGGGAGA ATCGGCTCAGATTTACTCAATCATCACTGTGGACAAGGGAACCTCTCTTAGCTTTCCGAAGACTCGTCTTTGGTGCCTCT GGTCTTGGTGCTCAAGTTGGGAACTGTTGGCTTCAATATGCAAAGCTTTGCCGTTTGGCTGGACACTATGAGACAGCTCA CAGGGCAATTTTGGAAGCTCAGGCTTCTGGTGCACCTAATGTTCACATGGAAAAGGCGAAACTTCTTTGGATCACTAAGC GTTCAGACAGTGCAATTATAGAGCTACAACAATCTCTCCTGAATATGCCTGAGGGTGTTGTTGACTCCACTGTAATTTCT TCGATCAATAGCTTACTGATGGCTCCCCCAAATCCAGAGCCCACAGTTCGTAACACACAATCTTTCAAAGAAAAAAAAGA TGTCGCAAAGACTCTTCTTTTGTATTCCAAATGGATCCATCACAGTGGGCAGAAACAAAAGAAAGATGTTTTAAATCTGT ATACCCAAGTCAAGGAATTGCTGCCCTGGGAGAAGGGATATTTCCATTTGGCTAAGTATTATGATGAACTATATGTTGAT GCAAGAAAGTGTCAACAAGAGAGCTCTGTATTTAGCTCTGCAGGCAGTAAAAAAGGATCTGTCTCGTCGAACTTAAGCAC TGAGAAAGCAGGGTGGGATTACCTGTTTAAGGGAATGTACTTCTATGCTAAGGCACTTCACAGTGGACACAAGAATCTCT TTCAAGCACTTCCGAGGTTGTTAACACTGTGGTTTGACTTTGGTACTATCTATAAAACAAGTGGCTCAGCGGGAAATAAG GAATTGAAAAGTACTCATATGAAGATAATGAGTTTAATGAGGGGCTGCTTAAAGGATCTGCCAACCTATCAATGGTTAAC TGTGTTGCCCCAGTTGGTTTCTCGAATCTGTCACCAGAATGCGGATACAGTACTAATGGTTAAAAATATCATCACTTCTG TTCTACATCAATTTCCTCAGCAAGGGCTCTGGATTATGGCAGCTGTTTCAAAATCTACTGTTCCTGCTAGAAGGGAAGCA GCTGCAGAAATCATACAAGGTGCACGAAAAGGTTTTAACCAAAGTGACAGAGGCCACAATTTGTTCATTCAGTTTGCTAG TTTGACTGATCATTTCATTAAACTATGCTTCCATGGAGGACAACCAAGATCCAAGGTTATCAATATAGCAACTGAATTCA GTGCCTTGAAAAGAATGATGCCGCTGGATATCATCATGCCAATTCAACAATCTCTTACCATTAGCCTACCAGCATTCCAT ATGAATAACAATGAACGACACTCTGCCAGTGTCTTTTCTGGTTCAGATCTACCAACTATTTCTGGTATAGCCGATGAAGC CGAGATACTTTCTTCCCTTCAGCGCCCAAAGAAGATCATTCTGCTGGGAAATGATGGTATTGAGTATCCATTCCTCTGCA AGCCCAAGGATGATCTCAGGAAAGATGCTCGGATGATGGAATTTACTGCGATGATAAACCGCTTACTATCCAAGTATCCC GAAAGCAGACGAAGGAAGCTATATATCCGCACCTTCGCAGTAGCTCCTCTGACTGAGGACTGTGGTCTGGTCGAGTGGGT TCCACATACACGTGGACTCCGCCATATTCTACAAGATATATACATTTCCTGTGGGAAGTTTGATCGGCAGAAAACAAATC CTCAAATTAAAAGAATATATGATCAATGTGCTGTTAAAAAGGAGTATGAGATGCTGAAGACCAAAATCCTCCCTATGTTC CCTCCGGTTTTCCATAAATGGTTCTTGACAACGTTTTCTGAACCAGCTGCTTGGTTTAGGTCACGTGTAGCTTATGCTCA CACCACAGCAGTTTGGTCCATGGTTGGGCACATTGTTGGGCTTGGTGACCGTCATGGAGAAAATATTCTCTTTGATTCTA CTTCGGGTGATTGTGTTCATGTAGACTTCAGTTGCTTATTTGACAAAGGCTTACAACTGGAGAAGCCTGAGTTGGTGCCA TTCAGATTGACCCAGAACATGATTGATGGTTTGGGCATCACTGGATATGAGGGCATCTTCATGAGGGTATGTGAAATCAC TCTCACAGTGTTGAGGACACATAGGGAGACGTTGATGAGCATCCTGGAGACATTCATCCATGATCCTCTTGTGGAGTGGA CAAAGTCACACAAATCAAGCGGAGTAGAAGTTCAGAACCCACATGCTCAGCGGGCCATAAGCAGCATTGAAGCCCGGCTG CAGGGCGTGGTTGTTGGTGTTCCGCTACCGGTAGAAGGTCAGGCTCGCCGGTTGATTGCCGATGCAGTCTCACTTGAGAA TCTTGGGAAGATGTACATCTGGTGGATGCCCTGGTTCTGATGAAAATCTCTGAACACCAGCTGTTGTTAAGTTCACACTA CACCAGTTTTCATAATGCCATTTATGTTAATACAGTAAGTTGACATGCAGATAACACTA ................................................................................ .........N.............................................N................N....... ................................................................................ ...........i.................................................................... ...........................................N.................................... ................................................................................ ................................................................................ .........................i..................................N................... ..........................N.........................i........................... ...............................................i................................ .....................N.......................................................... ................N................N.......i....N................................. ......................N.........................................N............... .................N..................................................N........... ....................................N........................................... ..........N.................................N................................... ...........................i.................................................... .......N........................................................................ ....N......................................N.................................... .....N........N........N...................i.................................... ..................................................i.........N................... .........N.......................i...N.......................................... .......i..................................................................N..... ...............N.................................................N.............. .........................................N...................................... .......................N.....................................i.................. ..........................N.........................................N........... .....................................N.................................N........ ........N.................N.................................N................... ...............N..................................i..............i.............. ........................................N..N.................................... ............................................................N................... ............N................................................................... ......................N......................................................... .......................................................i............N........... .....................................................................N.......... ..............N.............................................................N... .........................................N...................................... .......................i.............................................N.......... ........................................N....................................... ................................................................................ .......................................N.........i.............................. ............................N................................N.................. .................................................i......N.....N................. ...................................i..N.....................N.............N..... ......N...N..............N...................................................... ..N.......................N..................................................... .........N...........N.......................................................... .......................................................N........................ ..............................N.....N.....................................N..... ................................................................................ ...............................................................N................ ....N.......N..N................................................................ ............................................................................N... .......................N...........N..................N.............N........... ................i...................N........................................... .......i...........................................N............................ ...............................................................................i .......................................N...........N............................ .........................N...................................................... ...................................................i..........N................. ................................................................................ .......................i.......i................................................ ....................N.............i...............N............................. ..............................i.....N.............N............................. ................................................................................ .....................N..................i..............................N........ ..............i.................i.........i..................................... ................................N........................i...N...........N...... ..N...................................N.................N.................N....i ..i............................................................................. ...i............................................................................ .....N.......................................................................... ................................................................................ .....................................N.............................N............ ......................................N.......N................................. ............................................i................................... ..................i............................................................N ..............................N..................................N.............. ............................................................N..N........N.....N. ................................................................................ ..................................N.........N................................... ................................................................................ ..................N........N........N..................................N........ ......................................N................i........................ ...................................i............................................ ................................................................................ ........................i.......N............................................... ..............i..i.................N............................................ i.........i..............................................................N...... ..................................................N..N.......................... .........i..............i.......i..i..............N............................. ................................................................................ ................................................................................ ..................N......i...................N....i.......................N..... .................N.......................................................N...... ...................N.................................N.......................... ..................N............................................................. ..................i......N....................N.............N.......i........... ..................................N........................N.................... ..........................................N..................................... .............................................................N.................. ..........N..............N.............N........................................ ..............N........N....................N.............. Pos Score Pred ------------------------ 90 0.206 - 136 0.424 - 153 0.413 - 252 0.824 Yes 364 0.281 - 586 0.612 Yes 621 0.187 - 667 0.198 - 693 0.520 Yes 768 0.691 Yes 802 0.217 - 820 0.539 Yes 826 0.336 - 860 0.466 - 902 0.443 - 977 0.327 - 994 0.109 - 1002 0.766 Yes 1007 0.402 - 1063 0.293 - 1105 0.366 - 1138 0.154 - 1189 0.323 - 1237 0.170 - 1291 0.141 - 1325 0.203 - 1388 0.580 Yes 1448 0.367 - 1525 0.221 - 1564 0.172 - 1606 0.097 - 1615 0.134 - 1624 0.106 - 1644 0.513 Yes 1731 0.666 Yes 1741 0.266 - 1770 0.344 - 1794 0.657 Yes 1798 0.216 - 1848 0.663 Yes 1915 0.128 - 1936 0.152 - 1986 0.191 - 2042 0.107 - 2104 0.120 - 2142 0.711 Yes 2187 0.218 - 2229 0.226 - 2278 0.167 - 2312 0.254 - 2329 0.434 - 2347 0.440 - 2381 0.441 - 2416 0.129 - 2451 0.659 Yes 2466 0.678 Yes 2521 0.333 - 2524 0.181 - 2621 0.424 - 2653 0.103 - 2743 0.113 - 2856 0.609 Yes 2869 0.079 - 2950 0.282 - 2975 0.444 - 3037 0.390 - 3082 0.159 - 3144 0.775 Yes 3190 0.211 - 3241 0.376 - 3400 0.106 - 3410 0.682 Yes 3469 0.165 - 3502 0.410 - 3570 0.769 Yes 3577 0.137 - 3583 0.156 - 3636 0.555 Yes 3639 0.364 - 3661 0.256 - 3675 0.334 - 3687 0.321 - 3691 0.090 - 3706 0.309 - 3763 0.187 - 3787 0.283 - 3850 0.332 - 3862 0.151 - 3976 0.470 - 4002 0.524 Yes 4036 0.343 - 4041 0.390 - 4060 0.181 - 4071 0.411 - 4079 0.322 - 4111 0.265 - 4117 0.397 - 4155 0.270 - 4304 0.223 - 4325 0.145 - 4333 0.202 - 4336 0.219 - 4477 0.329 - 4504 0.195 - 4516 0.346 - 4535 0.348 - 4549 0.387 - 4577 0.647 Yes 4597 0.388 - 4648 0.537 Yes 4692 0.251 - 4800 0.642 Yes 4840 0.196 - 4852 0.198 - 4906 0.098 - 5012 0.807 Yes 5023 0.244 - 5144 0.513 Yes 5152 0.529 Yes 5221 0.446 - 5235 0.690 Yes 5251 0.269 - 5311 0.588 Yes 5317 0.228 - 5331 0.237 - 5462 0.130 - 5481 0.545 Yes 5512 0.281 - 5535 0.698 Yes 5553 0.548 Yes 5563 0.675 Yes 5633 0.185 - 5658 0.789 Yes 5662 0.433 - 5674 0.429 - 5683 0.134 - 5719 0.183 - 5737 0.131 - 5755 0.201 - 5760 0.757 Yes 5763 0.833 Yes 5844 0.687 Yes 5926 0.464 - 6118 0.329 - 6148 0.293 - 6199 0.081 - 6207 0.465 - 6285 0.658 Yes 6339 0.535 Yes 6400 0.436 - 6431 0.468 - 6466 0.309 - 6541 0.315 - 6544 0.260 - 6553 0.455 - 6559 0.306 - 6675 0.331 - 6685 0.121 - 6819 0.312 - 6828 0.425 - 6837 0.424 - 6872 0.434 - 6919 0.227 - 6936 0.748 Yes 6996 0.907 Yes 7145 0.525 Yes 7153 0.160 - 7215 0.526 Yes 7218 0.522 Yes 7236 0.349 - 7281 0.585 Yes 7291 0.670 Yes 7354 0.242 - 7411 0.494 - 7414 0.337 - 7450 0.578 Yes 7465 0.525 Yes 7473 0.528 Yes 7476 0.502 Yes 7491 0.287 - 7699 0.138 - 7706 0.711 Yes 7726 0.247 - 7731 0.686 Yes 7755 0.386 - 7778 0.270 - 7834 0.200 - 7860 0.489 - 7894 0.300 - 7939 0.306 - 8019 0.565 Yes 8026 0.278 - 8047 0.198 - 8061 0.312 - 8069 0.748 Yes 8115 0.333 - 8140 0.304 - 8203 0.125 - 8302 0.428 - 8331 0.161 - 8346 0.240 - 8360 0.166 - 8415 0.042 - 8424 0.029 - 8445 0.050 -

Explain the output -- Processing locus 4: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACGCAGGAGGAGGAAACATTATCTTCTTTGCAGTCTGGGTAGGCACTTTCTATTTATGAATAGTATTTCAACATTATCCT TTCTGTTGTCCTGAGAAATAACAATGGAAGTCGTTGCCTCAAATGTGCAGGAAAGTCGATGCAAAAATGGAAATGGACGA CCCAGAGAAGGCAATGGCTACAGTTGCTCAGCTCATAGAGCAACTTCATGCGAAAACATCTTCACCGCAAGATAAAGAAC TCACAACAGCTCGTCTACTAGGTATTGCAAAAGGCAAAAGGGAGGCAAGAAGGCTCATCGGTTCGTATGGCCAAGCAATG CCTTTATTTATTTCCATGCTTAGAAATGGGACGACTTTGGCCAAAGTAAATGTCGCTTCAATCCTTTGTGTATTGTGCAA AGACAAAGACTTGCGACTAAAAGTGCTTCTAGGAGGATGCATCCCGCCATTACTCTCGGTTTTGAAGTCCGGAACCATGG AAACTAGAAAAGCAGCAGCAGAAGCTATATATGAGGTTTCTTCTGCTGGTATTTCCAATGACCACATTGGAATGAAAATA TTTATCACAGAAGGTGTTGTTCCAACTTTGTGGGATCAACTTAGCCTGAAAGGAAATCAGGATAAAGTTGTTGAAGGGTA TGTTACTGGCGCTTTGAGGAATCTTTGTGGCGTTGATGATGGTTATTGGAGACTGACACTAGAGGGTAGTGGTGTAGACA TAGTTGTGTCTCTTTTGTCATCTGATAACCCTAATTCTCAAGCAAATGCTGCTTCTCTGCTGGCTCGTCTAGTTCTGTCC TTTTGTGATAGCATCCAGAAAATATTAAATTCTGGAGTTGTCAAGTCTCTGATACAGCTTTTGGAACAGAAAAATGATAT CAATGTTCGTGCTAGTGCAGCAGATGCTTTGGAAGCTCTTTCAGCAAATTCCGATGAGGCTAAGAAATGTGTCAAAGATG CAGGAGGTGTTCATGCTCTCATTGAAGCCATTGTTGCTCCATCGAAAGAGTGTATGCAGGGAAAACATGGACAATCTCTA CAAGAACATGCAACTGGAGCTTTAGCAAATGTGTTTGGTGGGATGCGCCATTTGATTATATATCTTGGACAAGTCTCACA ATCCCCTCGTCTAACGGAACCAATTGGTGATGTAATTGGAGCCCTTGCCTATGCTCTAATGATTTTCAAACAACCAGAGA GTTCCGAGAATATATTTGATCCAAGCGTGATAGAGAGCATTTTGGTAAAGTTACTAAAGCCTCGTGACACAAAGTTAATC CAAGAACGGATCTTAGAGGCTATGGCGAGCTTGTACGGAAACAGTAGTCTGTCGTGTTACCTAGATGATGCAGAAGCTAA GAGAGTTCTTATCGCTCTCATAACCATGGCTTCTGCTGATGTACGAGAGCGTCTGATAATTTGCTTGTCTGGCTTATGCC ATGACAAGGTTGGAATTTGGGAGGCGATTGGAAAGAGAGAAGGAATCCAGTTATTTATATCATTTCTAGGTTTGTCTAGT GAGCAACACCAAGAGTATGCTGTTGAAATGTTGAAAATTTTGACGGCTCAGGTGGATGATAGTAAATGGGCTGTAACAGC AGCCGGGGGGATACCTCCACTTGTCCAGTTGCTCGAGACTGGATCTCAGAAGGCCAAGGAAGATGCTGCCTGCATTCTCT GGAATTTGTGTTGTCACAGTGAGGAAATCCGTGATTGTGTTGAAAGAGCTGGTGGTATTCCAGCATTCTTGTGGCTTCTG AAGACTGGTGGACCAAATTCCCAGGAAACATCTGCAAAGACACTTGTGAAGCTTGTCCATACAGCTGATCCTGCCACAAT AAATCAGTTGTTGGCTTTACTTCTGGGAGATGATCCGACTTCAAAGATTCAGGTAATAGAAGTGTTGGGTCATGTGCTCT CAAAAGCTTCACAAGAGGATCTTGTTCATAGAGGATGTGCCGCTAACAAAGGACTGAGGTCCCTAGTCGAGTCCCTAACA TCTTCCAGAGAAGAAACAAAGGAGCATACAGCTTCAGTTTTAGCTGATCTGTTCAGCTCAAGACAAGATATTTGTGGTCA TCTTGCGACGGATGACATTATTAATCCCTGGATCAAGCTGCTTACAAACAACACTCAGAATGTGGCAAAACAGGTGGCTA GGGCTTTGGACGCACTGTCCCGCCCAGTTAAGAACAATAATAATAAAAAGAAGTCTTACATTGCCGAAGGAGATATTAAG TCACTCATAAAATTGGCTAAGAACTCTTCCATTGAGTCTGCAGAAAATGCGGTATCTGCCCTCGCAAATCTTCTGTCTGA TCCGGATATAGCTGCAGAAGCACTAGCTGAAGATGTTGTTTCCGCTTTCACAAGAATTTTGGCTGATGGATCTCCAGAGG GTAAGAGAAATGCGTCACGCGCACTTCATCAGTTACTTAAGAACTTTCCAGTTTGTGATGTGCTAAAGGGAAGTGCTCAG TGCCGTTTTGCTATACTTTCACTCGTTGATTCTCTAAAATCAATTGATGTGGATAGCGCAGATGCTTTCAACATATTAGA AGTAGTTGCGCTCTTGGCAAAGACTAAGAGTGGGGTGAATTTCTCTTACCCTCCATGGATTGCCCTTGCCGAAGTGCCAT CAAGCCTGGAGACTCTTGTGCAATGCCTTGCCGAAGGCCATACTCTTGTGCAAGATAAGGCAATAGAAGTTTTGTCCAGG CTTTGCTCTGATCAACAGTTTCTGCTCAGTGAACTTATAGTTTCAAGACCCAAGTCAATGTTGGTTCTGGCTGATAGAAT AGTGAATGCATCCAGCTTAGAAGTGAGAGTTGGGAGCACAGCGCTGCTCTTATGTGCTGCAAAGGAAAAGAAGCAGCTGA TCACGGAGACACTTGATCAGTCTGGTTTTTTAAAGCTCTTGTTACATGCCCTCGTTGATATGATAAAGCACAATTCTACA AGCTTTTCTCTAGAGACTGAAGTCCAGACACCTAAAGGTTTTTTGGAAAAAAATGTTTTCCAGGATACAGGTAGTTTTTA TTTTCCTGATCCAGCTAAAATCTTGGGTGGTACAGTCGCATTGTGGCTGCTATGCATACTCACTTCCGTTGATGCTAAGT CAAAGGTCATTGTTATGGAAGCTGGCGGACTTGAGGTGCTCGTGGGGAAGCTTGCTAGGTATACTTCCAGTGCTCAGGCT GAATTTGAGGATACAGAAGGCATTTGGATTAGTGCTCTCCTCCTGGCTATCATGTTCCAGGATGACAATGTGTCCTTTTC TTCAACGACAATGCGGATCATTCCAACACTTGCAGTTTTGCTAGGATCTGATGAACTGATAGATCGGTATTTCGCTGCCC ACGCAATGGCTAGTCTTGTTTGTACTAGGAACAGAGGAATAAATCTGACAATTGCAAACTCTGGTGCTGTTTCTGGGATA ATAAACCTTCTTGGCTATGTGGAATCGGAGATACTGAACTTGGTTGCTTTGGCAAATGAATTTTCTCTCGTGAAAGAGCC TGATCAAGTTATTCTTCAACACCTTTTTGAAATTGAAGATGTGAGGCTTGGCTCCACTGCACGTAAATCTATCCCGCTGC TTGTAGATCTCTTAAGGCCAATTCCAGATAGGCCTGGAGCCCCTCAGTTTGCTGTCCAAATCCTGATTCGCATTGCTGAT GGAAGTGACACAAATAAATTACTTATGGCTGAAGCTGGAGCTGTTGAAGCTTTAACAAAGTACCTTTCTCTAAGTCCTCA AGATTCAACTGAGTACGCCATATCTGAATTGCTTAGGGTATTATTTAGCAACCACGAGCTTAGACAAAACGAAATGGCAC TAAGTTCCTTGAATCAGCTTATCGCAGTACTGCGGCTAGGTTCACGAAGTGCAAGATACAGCGCAGCTGGGGCTCTGAAT GAACTATTTGATGCTGAAAACATCAGAAATTCTGAAATCGCCTGCCAGGCTGTTCAACCACTTATGGACATACTCGGTTC TGTATCAGAAAGTGAGCAAGAGGTAGCTCTCTCTGCATTGATCAAACTAAGCTCAGGAAATACTTCGAATACAGCTCTTC TGATTGATGTGGAAGGAAGTCTGCTGGAAAACGTGATCAAAATCTTATCATCTGCTACTGCTTCAGAGGAGTTAAAGATA AATGCTGCCAGACTTTGTTCTGTTGTTTTCTCGAATAAAAACATCAGAACAAGTGCATCCGCCTCCGGATGCATGAAGCC GTTAATAACACTTATGCAGTCTGAAAGAAGTGCAGCAGTTGAAGCAGCAGTTTTTGCTATTAAGATATTATTGGATGACG AGCAACATTTAGAACTAGCAGCCGCACATAACATTCAGGAGCTTCTTGTTGGCTTGGTTTCCGGGAAAAATTACGTTATT ATTGAGGCTAGCTTATCAGCTCTCATTAAGTTGGGAAAAGACAGAGTACCACGCAAGTTGGACATGGTGGAAGCTGGAAT AATTGAGCGATGTCTTGAGCTACTACCTGGAGCCTCGAGTTCGTTATGTTCCGCTGTTGTAGAGCTGTTTCGTATTTTAA CAAATAGTGGAGTAATCGCGAGAAGACCAGATGTTGCAAAAACTGTAGAGCCTCTCTTCGCGGTCTTGCTGCGGTCAGAC TTGACCTTATGGGGGCAACATAGTGCCCTGCAAGCACTCGTAAACATATTGGAAAAACAGCAAACCCTTGAAGCTTTTAG TTTTACACCCAGTGAAGCCATTGTGCCGCTGATTTCTTTTTTAGAATCTTCATCCCAAGCTATCCAGCAGCTTGGAGCAG AACTGCTGAGCCATTTTCTTACAATGGAAGATTTCCAGCAAGACATCACAACACAGAGTGCTGTTGTTCCTCTGGTCCGG CTTGCAGGAATTGGAATACTGAGCCTGCAGGAAACTGCGATAAAGGCATTGGAGAAGATATCTGCTAGCTGGCCTAAGGC TGTTCTTGATGCTGAAGGTATTTTTGAGCTATCCAAGGTTATTCTTCAGGAAGATCCTCAGCCCCCTCTTGATCTATGGG AGTCAGCTGCCTTTGTTCTTTCCAACATTCTGCAGTATGATGCAGAATGCTTTTTCAGAGTTGAACTGCCAGTGTTGGTA AAATTGTTGTTCTCGACAATTGAAAGCACAGTGTTACTGGCTCTTAAAGCTTTGATGCTTCATGAGAAGAACGACGCTTC AAGTACAGTACAAATGGCTGAGCTTGGTGCAATCGATGCATTGCTGGACCTCCTGAGATCTCATCAATGCGAGGAAGAAT CTGGAAGCTTACTTGAAGTAATATTTAACAATCCAAGAGTAAGAGAGTTGAAGTTATGCAAATATGCAATAGCACCACTA TCTCAATATCTGTTGGACCCTCATACAAGATCAGAACCTGGTAGGCTTCTTGCTGCTCTGGCACTGGGGGATCTATCTCA GCATGAAGGGCTTTCTAGATCCAGTGGTTCTGTTTCCGCTTGTCGGGCACTCATTAGCGTGCTAGAAGAACAACCAACAG AAGAAATGAAAGTGGTGGCCATCTGTGCGTTGCAGAATTTTGTGATGAATAGTAGAACGAATAGACGAGCTGTTGCAGAG GCTGGTGGCGTATTGTTAATTCAAGAGCTACTGCTTTCTTGCAACCCAGAAGTTTCTGGGCAGGCTGCACTGATGGTCAA ATTTTTGTTTTCCAACCACACACTCCAAGAATATGTCTCGAATGAGCTTATAAGATCACTAACAGCTGCACTTGAGAGAG GCTTGTGGTCTACAGCAACTATAAATATTGAAGTTTTGAGAACCTTAAATGTGATTTTCTCCAATTTTCCTAAGCTCCGT GCCTCAGAAGCTGCTACTTTCTGCATCCCTCATTTGGTAGGGGCGCTCAAATCTGGTGTTGAAGACGTCCAGGGATTAGT ATTGGATATTCTTTACTTGCTAAGACACTCCTGGACGAATATGTCTATAGATGTTGCAAAGTCTCAAGCCATGATTGCAG CTGAAGCAATTCCCGTCCTGCAAATGCTGATGAAAACATGCCCTCCTAGGTTTCACGACAAGGCAGATAGCTTATTGCAC TGCTTACCAGGTTGCTTAACAGTGAACGTCATGCGTGCCAATAACTTAAAGCAGTCTATGGCAACCACAAACGCCTTTTG TCAATTAACAATAGGAAACTGCCCTCCACGGCAAACTAAGGTCGTGAGCAATAGCACTACTCCTGAATGGAAAGAAGGCT TTACTTGGGCATTTGATGTTCCCCCCAAAGGACAAAAGCTTCACATCATATGCAAGAGCAAAAGCACATTTGGAAAGACG ACTCTAGGGCGAGTTACTATCCAAATCGACAAAGTCGTGACAGAAGGAGAGTACAGTGGATCTCTAAGCCTGAATCATGA AAATAGCAAAGATGCTTCTTCCAGATCACTCGACATTGAGATAGCATGGTCCAATAGGACAACGGATGAGACTCATTGAT CTTCACAAGCACAATGCACTTGGTCATTGGAAGACTGAAATCTGTCTAATGCTCCTAATGACTGCATTTTGGTAGTAGAA GAAGGAAAGCAAAGTCTG .......................................................N........................ .......................i..................i...............N.......i.....i....... .............i.................................N................................ ..................................................................N..........i.. ...............N.........N.......................i.............................. ....................................N.......................................i... ..............................N..........................N.............i........ ...............................................................................N ...................................N..N......................................... .............................................N.................................. .........................................................................N...... ..N....................N.............................N............i..........N.. ............N........................................N............N............. .......N....................N.............N..................................... .............................N....................N.......N..................... ................................................................................ .....................i..........................................N..N............ .........................i............N....................................N.... N............................................................................... ................N..........N...........................N.........i.............. ..............................................................i................. ................................................................................ ................................................................................ .............................N.........................................N........ ..................................N............................................. ................................................................................ ...........N...............................................N.................... ................................................................................ ..............................................N................................. ................................N................................N.............. .........N...............................................N...................... ..............................................N..............N.................. ......................................................N......................... ......................N......................................................... .........................................................i...................... .....N.............................................i............................ .............................................N.............N.................... ....................................................N........................... ...................................................N...................N........ ..............i................................................................. ...................................................N.........N.....N............ ..........i.......................................N............................. .....i.......................................................................... ................N......................................N........................ ......................................N......................................... ..............................................................................N. ........................i....................................................... .........................................................................i...... ..............................................................................N. ..........N....................................................i................ ................................................................................ ......N......................................................................... .............i............................................................N..... ................................................................................ ...............................................................i................ .........N...................................N.................................. ..............................N................................................. ........N....................................................................... ................................................................................ .......................i........................................................ ................................................................................ ........N..................................................................i.... ....................................N..N......i................................. ......................................................N......N.................. .............i.....................N..............................N............. .......................................................N.......N................ ................................................................................ ..i............................................................................. .....i......................................N................................... ........................................................................N....... ................................N........N...................................... ................................................i............................... ................................................................................ ........................................i.........N...................i......... .......................N.....N.......N.......................................... ..............................N..........................i...................... ..................................................................N............. ...............N.................................N.............................. ............................................................................N... ...........N.................................N...................N.............. .............N..................................N........N...................... .................. Pos Score Pred ------------------------ 56 0.328 - 104 0.766 Yes 123 0.554 Yes 139 0.246 - 147 0.775 Yes 153 0.869 Yes 174 0.612 Yes 208 0.164 - 307 0.369 - 318 0.539 Yes 336 0.433 - 346 0.395 - 370 0.645 Yes 437 0.335 - 477 0.571 Yes 511 0.352 - 538 0.409 - 552 0.646 Yes 640 0.072 - 676 0.081 - 679 0.143 - 766 0.157 - 874 0.468 - 883 0.195 - 904 0.153 - 934 0.154 - 947 0.742 Yes 958 0.230 - 973 0.138 - 1014 0.258 - 1027 0.421 - 1048 0.453 - 1069 0.471 - 1083 0.369 - 1150 0.119 - 1171 0.178 - 1179 0.240 - 1302 0.650 Yes 1345 0.226 - 1348 0.242 - 1386 0.715 Yes 1399 0.294 - 1436 0.251 - 1441 0.409 - 1537 0.289 - 1548 0.346 - 1576 0.150 - 1586 0.622 Yes 1663 0.512 Yes 1870 0.210 - 1912 0.213 - 1955 0.445 - 2092 0.121 - 2140 0.317 - 2287 0.160 - 2353 0.184 - 2386 0.369 - 2410 0.248 - 2458 0.181 - 2527 0.171 - 2542 0.208 - 2615 0.280 - 2663 0.385 - 2778 0.732 Yes 2806 0.161 - 2852 0.661 Yes 2926 0.257 - 2940 0.193 - 3013 0.423 - 3092 0.172 - 3112 0.234 - 3135 0.726 Yes 3252 0.260 - 3262 0.226 - 3268 0.277 - 3291 0.664 Yes 3331 0.174 - 3366 0.775 Yes 3457 0.138 - 3496 0.222 - 3559 0.359 - 3679 0.291 - 3705 0.599 Yes 3834 0.711 Yes 3919 0.160 - 3931 0.250 - 3984 0.551 Yes 4087 0.127 - 4162 0.515 Yes 4229 0.200 - 4233 0.490 - 4254 0.652 Yes 4315 0.275 - 4464 0.504 Yes 4490 0.482 - 4526 0.147 - 4591 0.485 - 4649 0.452 - 4824 0.617 Yes 4969 0.078 - 5036 0.563 Yes 5077 0.159 - 5080 0.117 - 5087 0.689 Yes 5175 0.478 - 5182 0.199 - 5214 0.830 Yes 5236 0.198 - 5267 0.470 - 5336 0.361 - 5344 0.322 - 5443 0.525 Yes 5526 0.645 Yes 5565 0.486 - 5673 0.318 - 5713 0.258 - 5722 0.183 - 5809 0.512 Yes 5961 0.695 Yes 5971 0.179 - 5991 0.694 Yes 6024 0.397 - 6030 0.151 - 6038 0.462 - 6111 0.473 - 6138 0.744 Yes 6227 0.409 - 6256 0.151 - 6290 0.388 - 6397 0.324 - 6412 0.283 - 6446 0.429 - 6466 0.316 - 6494 0.177 - 6529 0.067 - 6538 0.037 -

Explain the output -- Processing locus 5: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCGAGGAGGCGCATGTTAACACACCATGCGCACGTTAAATCCTGTCTTTGCCCTCTCTCTGTAGCGAAACAAAAAAAAG AAAATTGATCAACCTAGATAAAATAGCAGAAGAGAAGAAGCTGTGAGAGGAAAAAAAAAAAAATTGAAGACAAAAACCGC CATTGAAATGATGAAGCAAAGCTCATCGGAGGCAGTGTCATCGTCTTCTTCATCAGCCGCCGCGGACCAATCTCATCCCT CCGTTTCATCATCATCATCGGAGAAGCCTCTATCTTCCTCTTCGATTTCTCCAGCGGAGGATCTTCAGGTTGGATCGTCG AGAGATGGAAGCGGAGGTGCACAAGAGATCGTAGCCGTCGATCGTCCAGGAGAGTACTCGGCGTTGTGTAGATGGACGGT TGAGAGCTTCACACGTGTCAAAGCTAAGGCACTGTGGAGCAAGTACTTCGATGTAGGAGGATACGATTGTCGTCTCTTGG TTTATCCTAGAGGTGATTCTCAGGCTCTTCCTGGTTACATCAGCATCTATCTTCAGATCATGGACCCTAGAGGAACCACT TCGTCTCGTTGGGATTGTTTCGCTAGCTATCGTCTCTCTATCGTTAATCTTGTCGATGATTCTTTAACAATCCATAAGGA TTCTTGGCATAGATTCTCTAGTAAGAAGAAATCTCATGGATGGTGTGATTTCACCTTGAACTCTAGCATTTTGGATCCTA AGATGGGTTTTTTGTTTAACAATGATTCGTTGCTTATCACTGCTGATATCTTGATTCTAAACGAGTCTGTGAGCTTTAGT CGTGATAATAACAATGAGGGGCAATCTTTGTATAAGGAAAATTCCATAGCTGGACCAATGCCGGATGTGTTGAGTGGGAA GTTCACTTGGAAGGTAAATAATTTTAGTTTGTTTAAGGATATGATAAAGACGCAGAAGATTATGAGCCCTGTTTTCCCAG CTGGGGAGTGTAATTTGAGGATCAGTGTGTACCAGAGTGTTGTTAATTCGCAAGAATATATATCAATGTGTTTGGAGAGT AAAGAGACTGAGAAGACTTTGGTTTCAGATAGGAGCTGTTGGTGTTTGTTTAGGATGTCAGCTTTGAATCAGAAGCCTGG TTGCACTCACATGCATAGGGATTCATATGGCAGGTTTGCTGCTGATAATAAGAGTGGGGACAATACGAGCTTGGGCTGGA ATGATTACATGAAAATGTCTGAGTTTGTGAACCCGGAAGCTGGGTTTTTACTCGATGATATGGCTGTCTTTAGCACTTCG TTTCATGTTATTAAAGAGTTCAGTAGCTTTACTAAGAATGGTGGGTTGATTGGGGGAAGGAATGGGGCTGGAGCTAGGAA GTCAGATGGACACATGGGAAAATTCACTTGGAGAATTGAAAATTTCACAAGGTTAAAGGATCTTCTAAAGAAGAGGAAGA TAACAGGTCTTTGCATCAAAAGTAAGAGGTTTCAAATTGGGAACCGGGATTGTCGACTTATTGTTTATCCCCGAGGGCAG TCCCAGCCACCATGCCATCTTTCAGTATTTCTTGAAGTGACAGATTCACGAAGCTCAAGTGATTGGAGCTGTTTTGTTAG CCACCGACTATCAGTTGTGAACCAGAGATTGGAGGAGAAGTCGGTGACAAAAGAATCTCAGAATCGCTACTCGAAAGCTG CAAAGGATTGGGGTTGGCGTGAGTTTGTGACACTTACTAGTTTGTTTGATCAAGACTCTGGCTTTCTTGTTCAAGACACT GTTGTTTTCTCCGCTGAGGTTCTTATTTTGAAAGAGACATCTGCTACAAAAGAGTATGTGGAGGCAGACTCAACTAATTC AGTTTCTCCGACTGATAACAGCGTCAAAAAAAGTTCATTTACTTGGAAAGTGGAAAATTTCTTAGCCTTCAAAGAGATAA TGGAAACACGAAAGATTTTTAGCAAATTCTTTCAGGCTGGTGGATGTGAACTCCGGATTGGTGTATATGAGTCCTTTGAC ACCATATGCATATATTTAGAGAGTGATCAATCTGCGGGTACAGATGTGGACAATAATTTCTGGGTTAAGTACAAGATGGG TATTCTGAACCAAAAGAATCCGGCCAAAAGTGTGTGGAAGGAGTCATCTATATGTACCAAGACATGGAATAATTCCGTTC TACAGTTTATGAAGGTGTCTGACATGTTGGAGGCTGATGCTGGGTTTCTTGTGCGTGACACAGTTGTGTTTGTGTGTGAA ATATTGGATTGTTGTCCTTGGTTTGAGTTTTCAGACCTAGAGGTACTGGCTTCAGATGATGACCAAGATGCCTTAACCAC TGATCCTGATGAAATTATTGATTCTGAGGAAAGTGAGGGCATAAGCGGAGATGAGGAAGACACATTTCGAGATTTTCTTT CCCGAGCTGGATTCCATCTCACGTTTGGGGAGAATCCTTCACAACCACAAGTTACTCTCCGTGAGAAGCTTCTAATGGAT GCGGGTGCAATTGCTGGGTTTCTTACTGGCTTGCGTGTATATCTTGATGACCCAACTAAAGTAAAGCGTTTACTTCTTCC GACTAAAATATCTTGTAATGATAGAAGCAAGCTAATAAAGAGTGATGAATCTTCCCCTAGCCTAATGAATTTGCTGATGG GTGTTAAAGTTCTACAGCAAGCCATTATTGATTTACTTCTAGATATTATGGTTGAGTGTTGCCAACCTTCAGAGGAAGGC TCTCACTCTGAGCCGTCTTTATTATCCCCAAAGACAGATGGTTGCGTGGCTGCAACTTCATCGGAGTCAACAAGGGAACG AGGAGCAGCAGAACCTTCGCAACATCTTGTTGACGAAAGATTTAAATCTGATACAGATGCTACCCTCAGTACCTCAGCTG TACCAAGCTCAGAGATGAATGGGATCGATTTGCTTGAGAAAGCCCTTGCAATAGAGCCTATGTCTCCTCCTACCACATCT GCTGGGCAATCTTCTGATGCTTCAGTCCAATCAAAGACAAAATGGCCGGAACAGTCTGAGGAGCTATTGGGACTGATTGT GAATTCACTTAAAACCCTTGATGCGGCTGTTCCACAAGGTTGTCCAGAGCCTAGACGACGACCACACTCTGCTCAAAAGA TTGCTCTTGTTTTAGACAGAGCACCCAAACATTTGCAACCAGATTTAGTTAGTCTGGTTCCCAAATTGGTTGAGCATTCA GAGCACCCACTGGCTGCTTTAGCACTTATAGAACGGCTTCAAAAGCCTGAAGCTGAACCAGCGTTGCGTGAACCAGTATA CAATGCTCTTAGCCAGTTAGAATGCGACAGTGAAGTGTGGGAGGACATTCTATCTCAGTCTTTTGAGCTTTTGAGCGACT TAAATGAGGAATCTCTTGTGGCGGCCATTCATTTCACATTTAAGACTGCCTCCCAGTGCCAACACCTACCTGAAGCCGTA AATGCAGTCCGTGAACGGCTTAAAAGTCTGGGTGCTGATGTGTCTCTTTGTGTCCTTGACTATTTGAGTAAAACCGTACA CAGTTGGCCTGGTATTGCCGAAATGATACTTAGGGATATCAACACTGACGACAGTGTAGGTGCCAATTTTGCAACATTAC CCTGCGGGCCTTTTCTGATTGGTGAAAATGAGACTGCCTCCGAAATGGCGGATTTGATGGAAGAACATATATTCTATGCA AATAGGCAGTTTTTTGATGTCTACATTCTGCTGGAAATGTTGTCCATATCGTGCCTAGCTGAAGAGGCATCTCAAACATT TGAAAGAGCTGTTGCTCGTGGAGCCATTGTTGCTCAGGCTGTTGCCATGGTCCTTGAAAAACAACGTGTTCAGAGCCCAA ATCTGAATGCTACAAGTGGGGATGCTTCTCTTAAGCATCAAGATCCTGTATTGGAGGGAGAAGCCAGTGAGCAACCAGCC ACTGGAGGGACTGAGTTCAGGATAATCCTTAATCTTGCTGAGACATTAACTCATTCTAGAGATCCTCAAGTGAGGGGATT TGTGAAAATGCTATACACAATATTATTCAAGTGGTTTCCGGATCAGCCCTTTAGGGTTCAAATACTGAGGAGACTCGTAG ACAGATTCACCAGCCCTACGAGTAGCAGCCACGATTTAGACTTAGAGTTGGAGATTCTAGCTATTCTGATTTTTCAGGAG CAAGAGGTTGCGAGACCTGTTTTAGCCATGCTGAAGAAGGTTGTGGAACATGCAAATATTGACAGGGCGGCCCTGTGGCA CCAATTGCGTGCAAACAAGGAAGAACTTGTCCGGTTGAAGGAAGAAAAGAAGATAGAGATTCAATCTATGACAAAGGAGA AATCTAGCATCACCCAAAAATTAAGTGAATCTGAGGCTGCAAATACCCGTCTTAAGTCTGAAATGAAAGCTGAGGCGGAC CGGTTTTCTCGAGAAAAGAAGGATCTGGTAGAACAGTTTAGGGATGTGGAGAGTCAGCTGGAATGGATCCGATCAGAACG TCAAGATGAGATAGATAAGCTCTCGTCTGAAAAGAAAACTCTTTTGGATCGCCTTCACGAAGCAGAGACGCAACTTGCTT TGCAAAAAACCCGAAAGCGTGATGAACTCAAGAAGGTAGGAAAAGAGAAAAATGCTCTAACCGAGAAGCTAAAGGTAACA GAGGCAGCTCGGAAAAGATTTGAAGAAGAGTTGAAACGGTATGCCACTGAGAATGTAACTCGGGAGGAATTACGCAAGTC ACTTGAGGATCAAATCAGGCAATTGACACAGACAGTAGGACAGACAAAAGAAGAGAAACGAGAGAAAGAAGATCAGATTG CTCGGTGTGAAGCTTACATTGATGGAATGGAATCCAAACTCCAAGCTTGCCAGCAATACATCCACACACTAGAATCCTCG CTCCGAGAGGAGATTTCAAGACATGCGCCTCTGTACGGTGCCAATTTAGAGTCTCTATCGATGAAGGAACTGGATACAAT AGCTCGTATCCATGAGGAAGGGCTAAGACAGATCCATGCCCTTCAACAACGTAAGGGCCATACATTATCACACGGTCTCC CTCAGGGTCACACACTTTACCCGACTACACCGCCTCAGCTTCCACCAATGGCAATCGGTTTGCCACCTCAGCTTATACCT AATGGTTCAGGGGTTCACTCAAACGGTCATATCAACGGTTCGGTCAGGCCTTGGTTTAGTCACCACACCTAAAAAAATAC ATCTTTTTTTTCATAGGAAGACTACACAAAAGAATGATGGTCTCTTGAGTTTGTGAGGTAAATCAATCAATCGATCAACA TCATCAATGTT .............N............i..................................................... ................................................................................ .......N..N..................................................................... ................................................................................ ....i..................................................................N........ ..................................................i............................. ...........................................................i.................... .......................................................N........................ ...................................N...N........................................ ..i..................N.......................................................... .............N...........................................i......N............... ........................................N....................N.................. .................................................................N.............. ......................................................i......................... ..........i...............N..................................................... N.......i.....i.......................................N....i.................... ....N................................N.......................N.................. .....N.......N.................................................................. ................................................................................ ...........N.................................................................... ................................................................................ ................................................................................ .......................................................N........................ ...............................................................................N ...........................................N......................N............. .....N.....................................N...............................N.... ...................................................N...........N................ ........N..............N............N........................................... .......................................................N..N........N............ ........N.........................................N............................. ..........................................................................N...N. ..............................................N................................. .................N..........................N...................N...........i... ...............................................N................................ .....................................N.......................................... ........................................................N....................... ..............i...N........................................N.................... ................N........................i...................................... ....................N........................................................... ................................................................................ ................................................................................ ..N..................N.......................................................... ...N............................................................................ ......................i......................................................... ...........................N................i...........N..................N.... ................N...................i........................................... ..............................................i................................. ......N..............N.......................................................... ................................................................................ .......i........................................................................ ................................................................................ ...........................N.....................N.............................. ...................................................................i............ ..............................................................i................. ...........................................N..................N................. .....N.......................................................................... .....................N.............................N............................ ........................................N...........N........................... ................................................................................ .....................N....i..................................................... ......................N.....................................i................... ...........i.......................N............................................ ...............................................i................................ .................................N..N........................................... ......N.... Pos Score Pred ------------------------ 14 0.410 - 27 0.801 Yes 168 0.321 - 171 0.358 - 325 0.688 Yes 392 0.318 - 451 0.590 Yes 540 0.714 Yes 616 0.174 - 676 0.197 - 680 0.321 - 723 0.826 Yes 742 0.278 - 814 0.253 - 858 0.572 Yes 865 0.216 - 921 0.252 - 942 0.336 - 1026 0.450 - 1095 0.678 Yes 1131 0.502 Yes 1147 0.180 - 1201 0.146 - 1209 0.521 Yes 1215 0.651 Yes 1255 0.184 - 1260 0.813 Yes 1285 0.171 - 1318 0.419 - 1342 0.317 - 1366 0.147 - 1374 0.483 - 1532 0.201 - 1816 0.368 - 1920 0.422 - 1964 0.208 - 1987 0.197 - 2006 0.367 - 2044 0.334 - 2076 0.348 - 2132 0.256 - 2144 0.384 - 2169 0.299 - 2184 0.490 - 2197 0.424 - 2296 0.321 - 2299 0.101 - 2308 0.250 - 2329 0.078 - 2371 0.241 - 2475 0.496 - 2479 0.162 - 2527 0.197 - 2578 0.225 - 2605 0.190 - 2625 0.497 - 2637 0.523 Yes 2688 0.337 - 2758 0.474 - 2857 0.188 - 2895 0.751 Yes 2899 0.436 - 2940 0.447 - 2977 0.167 - 3002 0.836 Yes 3061 0.344 - 3283 0.280 - 3302 0.264 - 3364 0.225 - 3442 0.186 - 3478 0.430 - 3543 0.750 Yes 3628 0.116 - 3645 0.533 Yes 3657 0.308 - 3676 0.145 - 3697 0.216 - 3717 0.592 Yes 3807 0.544 Yes 3847 0.254 - 3862 0.244 - 4008 0.589 Yes 4188 0.485 - 4210 0.168 - 4308 0.567 Yes 4383 0.588 Yes 4444 0.388 - 4463 0.385 - 4486 0.355 - 4582 0.252 - 4612 0.264 - 4681 0.302 - 4693 0.257 - 4822 0.304 - 4827 0.518 Yes 4903 0.369 - 4941 0.730 Yes 4972 0.565 Yes 4996 0.351 - 5088 0.715 Yes 5122 0.499 - 5234 0.104 - 5237 0.209 - 5287 0.060 -

Explain the output -- Processing locus 6: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCGTTGTTACCGGAAGTAAAGTTTCTGAACGACGACGCAGAGAGCGATAGTATCCTCCGCCTGAGGAGCATTCCTTCGCA TGATCTCTCGACGGTGACTGACCAACACCGAAGCATCCAAAAACTCGAAAAACCCTAGCATTTTTCAACCAATCAGTGGC GCCCACGTCATACTCGATGTCGTGGCTTTTGTACGTTATTTACGAAAATGTCCTTTTAGTGTTAGTAAGGATATTTCGAT TCCCGGCTCGTTCGTTACTGGTTTTTTTCTTCTTCTCCGTCGCGGAAAAGATGGGGACCACCACCAATCTGAGATCTTGG CTGTATCTCATTCTTCTCTTCATCGTCGTCGTCGGCGTCAACGCTCAGAATCGGAGACCTAAGAATGTTCAGGTGGCTGT TAAGGCTAAGTGGCAGGGTACTCCTCTTCTCCTTGAGGCTGGTGAGTTGATTTCCAAAGAATCGAAGCAGTTGTTCTGGG AGTTTACTGATGCTTGGCTTGGTTCCGATGGAGATGATTCCGATTGTAAATCAGCTAGAGATTGTCTTCTTAAGATATCT AAACAGGCTTCGACTCTGTTAGCTCAACCAGTGGCTTCTCTCTTCCATTTCTCACTTACTCTGAGATCTGCTTCGCCTAG GCTTGTGCTTTACCGTCAATTGGCTGATGAGTCTCTATCTTCTTTCCCTCATGGTGATGATCCCTCTGCGACTGGCTGCT GTTGGGTTGATACTGGCTCCTCTCTGTTTTACGATGTTGCAGATCTTCAATCCTGGCTTGCCTCTGCTCCTGCTGTTGGA GATGCTGTTCAGGGGCCTGAGCTATTCGACTTCGATCATGTTCATTTTGATTCAAGAGCTGGAAGCCCAGTTGCTGTTCT CTATGGGGCTGTTGGGACTGATTGCTTTAGGAAGTTTCACCTGTCGCTTGCTAAAGCAGCCAAGGAGGGAAAAGTTACAT ATGTTGTTAGACCAGTATTACCTTTGGGTTGTGAAGGGAAAACTAGACCCTGCGGTGCCATTGGAGCAAGGGATAATGTT AGCTTAGCTGGTTATGGTGTGGAGCTTGCTTTGAAGAACATGGAGTACAAGGCTATGGATGATAGTGCTATAAAGAAAGG TATCACTCTTGAAGATCCTAGGACTGAAGATCTTAGTCAAGATGTTAGGGGCTTTATATTCTCAAAAATCTTGGACAGGA AACCCGAGCTAAGATCAGAAGTCATGGCTTTTAGGGATTATCTCCTATCATCAACAGTATCAGACACGCTTGATGTTTGG GAGCTGAAAGATTTAGGACACCAAACAGCGCAGAGAATAGTCCATGCCTCTGATCCTCTGCAGTCTATGCAAGAAATTAA TCAAAACTTTCCTAGTGTAGTTTCTTCATTATCTCGCATGAAGCTCAATGAATCAATCAAAGACGAGATACTTTCAAACC AGAGAATGGTTCCCCCTGGCAAGGCTTTGTTGGCTCTGAATGGTGCCCTGTTGAACATCGAAGATATTGATCTATACATG TTGATGGATTTGGCTCATCAGGAATTGTCCCTGGCCAACCATTTTTCGAAGCTTAAGATACCTGATGGAGCCATAAGGAA ACTTCTACTAACTACCCCTCTCCCTGAACCGGATTCATATCGGGTTGATTTCCGATCCGTGCATGTTACCTATCTCAACA ATCTGGAAGAGGATGATATGTACAAGCGTTGGCGGAGTAATATTAACGAGATTTTGATGCCTGCCTTCCCTGGACAACTA CGTTACATCCGAAAGAACCTATTCCATGCAGTTTACGTCATTGATCCAGCGACTGCCTGTGGTCTTGAGTCTATTGAAAC TCTCAGATCTTTATATGAAAATCAACTCCCTGTGAGATTTGGGGTGATATTATATTCAACTCAACTAATCAAGACAATCG AGAATAATGGTGGTCAAATCCCATCTTCTGATGCAGTGACTAATGCCCAAGTTAAAGAGGACTTATCGACTATGGTCATA CGTCTTTTTCTGTACATCAAGGAGCACCATGGGATTCAAACAGCTTTTCAGTTCTTGGGCAATTTAAACACACTAAGGAC TGAATCTGCTGATTCTTCCGAAGCAGACATTGAACAGGAGCACGTTGATGGTGCATTTGTTGAGACAATTTTACCAAAGG TTAAAACACTCCCTCAGGATATATTGCTGAAACTGCGACAAGAGCACACTTTGAAGGAAGCATCTGAAGCAAGCTCCATG TTTGTATTTAAGTTGGGTCTGGCGAAATTGAAGTGTTCCTTTTTGATGAATGGCCTTGTCTTTGATTCTGTTGAGGAAGA AACTCTTTTAAATGCCATGAATGAAGAGCTTCCCAAGATACAAGAACAAGTTTATTACGGCCAGATAGAATCCCATACTA AGGTTTTGGACAAGTTGCTTTCGGAAAGTGGTCTTAGCCGTTATAATCCACAGATTATTAGTGGAGGAAAGAACAAACCA AGGTTTGTGTCGCTAGCTTCATCTACCAGGAAAGGGGAATCTATGTTGAATGATGTTAACTACTTGCATTCTCCTGAAAC TTCAGAGGATGTGAAGTATGTGACTCATCTCCTTGCTGCTGATGTTGCAACGAAGAAAGGAATGAAATTGTTACATGAAG GAGTCCGTTACCTGATTGGAGGTTCCAAAAGTGCTCGTCTTGGAGTCTTATTTAGTAGCAGCCAGAATGCTGACCCACAT AGCCTTCTATTTATAAAGTTTTTCGAAAAAACTGCTTCATCGTTTAGTCATAAGGAAAAGGTGCTATACTTCTTGGATAA ACTATGCTTGTTTTACGAGCGTGAATATCTGTTAAAAACATCAGTGGAATCAGCTAGCTCTCAAATGTTTATTGATAAAG TCCTTGAGCTTGCTGATGAGTATGGGCTATCTTCTAAGGCATACCGATCTTGTCTCGTTGAATCAGTGGATGAAGAATTA CTTAAGCGTTTGACAAAGGTCGCCCAATTCTTGTCTTGGGAACTTGGACTTGAATCTGATGCAAATGCAATAATTTCAAA TGGAAGGGTGATATTTCCTGTGGATGAGAGGACATTTTTGGGCCAAGATTTACATCTTCTTGAGTCAATGGAGTTCAATC AAAGAGTAAAGCCTGTTCAAGAAATTATTGAAGGCATTGAATGGCAAGATGTGGATCCCGATCTGCTGACAAGTAAATAT TTTAGCGATGTCTTCATGTTTGTGTCATCTGCAATGGCAACACGGGATCGTAGTTCTGAAAGTGCGCGGTTTGAGGTGTT GAATTCAGAATACAGTGCGGTTTTGCTGGGAAATGAAAATGCAACGATACACATTGATGCTGTTATCGATCCTTTAAGCC CTACTGGTCAAAAACTAGCATCACTTCTTCAAGTTCTACAGAAACATGTTCAGACGAGCATGAGGATTGTTCTGAATCCC ATGAGTTCCCTTGTGGACATTCCTCTGAAAAATTACTACAGATATGTCTTACCTAATACGGACGATTACAGCAGTACAGG CTTTGACGTAGATGGGCCTAAGGCATTTTTCGCAAATATGCCATTGTCTAAGACACTTACCATGAATCTTGACGTTCCAG AGCCATGGCTGGTTGAACCAGTAATTGCCATTCATGATCTTGATAATATATTGCTTGAGAATCTAGGAGATACCACTACA CTGCAGGCAGTTTTTGAGGTTGAATCTCTTGTTCTTACAGGTCATTGCGCGGAAAAAGATCATGAGGCTCCTCGTGGCCT TCAGCTGATTCTGGGAACCAAAAATAGACCGCATTTGGTTGATACCCTTGTAATGGCCAATTTGGGTTATTGGCAGATGA AAGTATCCCCAGGGGTTTGGTATTTGCAACTTGCTCCGGGTAGAAGTTCGGAGCTATACGCTTTGAAAGGAGGAAATGAT GGGAGTCAAGATCAATCCTCGCTGAAACGTATAACTATCGATGATCTGCGTGGTAAAGTTGTTCATCTAGAAGTAGTTAA GAGAAAGGGTAAGGAGCATGAAAAGTTGCTAGTTCCTTCAGACGGTGATGATGCTGTGCAACAAAACAAGGAAGGAAGCT GGAACTCAAATTTCTTGAAATGGGCGTCTGGTTTTGTTGGTGGTCGTCAACAATCAATGAAGGGAGGTCCTGACAAAGAG CATGAGAAGGGTGGGCGACAAGGGAAAACGATAAATATATTCTCCATTGCTTCAGGGCATTTATACGAGCGTTTCCTTAA GATTATGATTCTCAGTGTCCTGAAGAACACCAATCGTCCTGTAAAATTCTGGTTTATTAAGAATTATCTCTCTCCTCAAT TTAAGGATGTGATTCCACATATGGCACAAGAATACAACTTTGAGTATGAGCTGATTACATACAAATGGCCGTCCTGGTTG CATAAACAGAAAGAAAAGCAACGAATTATTTGGGCCTACAAAATTCTTTTCCTTGATGTCATATTTCCTCTCTCATTGGA AAAGGTTATATTTGTCGACGCAGATCAAATTATAAGGACAGACATGGGAGAACTGTATGATATGGATATTAAAGGAAGAC CTCTCGCGTATACTCCTTTTTGTGACAACAACAGGGAAATGGATGGCTATAAATTTTGGAAACAGGGTTTCTGGAAAGAA CATTTACGTGGTAGGCCATACCATATCAGTGCTCTATACGTTGTTGACTTGGTTAAGTTCCGAGAGACAGCGGCAGGAGA TAATCTAAGAGTATTCTATGAGACGCTTAGCAAGGATCCAAACAGTTTGTCCAACCTAGATCAGGATCTTCCTAATTATG CTCAGCATACTGTTCCTATATTTTCACTTCCCCAAGAGTGGTTGTGGTGTGAATCCTGGTGTGGAAACGCCACAAAAGCC AAGGCCAGAACAATCGATCTTTGCAATAATCCCATGACAAAGGAACCAAAGCTTCAGGGTGCCCGAAGAATCGTAACCGA ATGGCCCGATCTTGATCTCGAAGCACGAAAATTCACAGCAAAAATCTTGGGTGAAGATGTGGAACTCAACGAGCCAGTAG CTGCTCCTGCTACAGACAAGCCTAATCCTCTTCCATCAAATGACATCTCTGAAGATACAGAACAAGACTTGGAATCTAAA GCAGAACTGTAAATTCAATGCTCCTGAAGTTTCAAAAGAGGAGAAGATAAATTAGACAATCATGGCTATTTTGCATTTGC TAGATAAGGTTTCTAGTTTTGTAGCTACAGT ...............................................................................N ................................................................................ ................N..............................i................................ ..................................................i............................. ................................................................N............... ................................................................................ .........N.................N.....N.............................................. ................................................................................ ..........................N.......................N.....N....................... .................................N.............................................. ..N............................................................................. N..........................................................................N.... .............N.........................i..............i...N..................... .........................................N...................................... .......................i................................................N....... ...........................................N......................N............. .....................................N.........N................................ .....i.................................N.....................................N.. ...N............................................................N............... ..............................................................N................. ............N....i......................................i....................... .........................N...................................................... ..............N................................................................. ......N.......................N...........N............................i........ ............................N................................................... ...............................................N................................ .............................................................................N.. .............................................N...N.............................. ...........N....N...N........................................................... ................................................................................ ..........................................i......N..N........................... ........N........N.......................N...................i............N..... ..................................................................N............. ................................................................................ ...N............................................................N............... ...............N.....N...............................................N.......... ..........................................................N.....N..............N .......................N...........................................N............ ........................................i.......N............................... .......N.......N.................i.............................................. ................................N.....N.................N....................... .............................................N.............i.................... N..........................................N.................................... ...........N.........................i.......................i.................. ....N............................N.............................................. .............................................................N.................. ....................................................N.......................i... ...........................................................................i..N. ........................................N....................................... .................N.............................N..N............................. ...................N....................................N....................... ....N........................................................................... ......N.............i........................N..................i............... .......................................................N........................ ...........................................i............N....N.................. ......................................N...N..................................... ................................................................................ .................N...........................................................N.. ................................................................................ .................................i.............................................. N.......................................................N....................... .......................................N........................................ .................N...........................................N.................. ............................... Pos Score Pred ------------------------ 80 0.275 - 177 0.227 - 208 0.592 Yes 291 0.769 Yes 385 0.402 - 490 0.343 - 508 0.283 - 514 0.135 - 667 0.337 - 691 0.215 - 697 0.126 - 754 0.136 - 802 0.226 - 838 0.281 - 883 0.099 - 961 0.493 - 1036 0.234 - 1054 0.100 - 1080 0.591 Yes 1095 0.580 Yes 1099 0.418 - 1162 0.167 - 1224 0.869 Yes 1273 0.289 - 1324 0.412 - 1347 0.278 - 1398 0.422 - 1408 0.195 - 1446 0.532 Yes 1480 0.128 - 1518 0.321 - 1524 0.468 - 1585 0.225 - 1663 0.197 - 1693 0.204 - 1698 0.521 Yes 1737 0.620 Yes 1786 0.406 - 1855 0.078 - 1927 0.414 - 1951 0.117 - 1963 0.213 - 1992 0.531 Yes 2029 0.410 - 2128 0.141 - 2238 0.355 - 2286 0.326 - 2290 0.489 - 2332 0.259 - 2337 0.213 - 2341 0.202 - 2523 0.758 Yes 2530 0.183 - 2533 0.167 - 2569 0.472 - 2578 0.283 - 2602 0.163 - 2622 0.662 Yes 2635 0.319 - 2707 0.276 - 2804 0.424 - 2865 0.285 - 2896 0.129 - 2902 0.270 - 2950 0.210 - 3019 0.087 - 3025 0.231 - 3040 0.459 - 3064 0.114 - 3108 0.455 - 3161 0.672 Yes 3169 0.255 - 3208 0.234 - 3216 0.411 - 3234 0.755 Yes 3313 0.156 - 3319 0.258 - 3337 0.244 - 3406 0.195 - 3420 0.656 Yes 3441 0.356 - 3484 0.275 - 3532 0.114 - 3558 0.772 Yes 3582 0.710 Yes 3605 0.485 - 3634 0.202 - 3742 0.262 - 3813 0.397 - 3837 0.839 Yes 3916 0.554 Yes 3919 0.296 - 3961 0.225 - 4018 0.106 - 4048 0.303 - 4051 0.407 - 4100 0.294 - 4137 0.293 - 4162 0.271 - 4245 0.313 - 4327 0.358 - 4341 0.572 Yes 4366 0.218 - 4385 0.577 Yes 4456 0.123 - 4524 0.760 Yes 4537 0.245 - 4542 0.410 - 4599 0.395 - 4603 0.220 - 4738 0.147 - 4798 0.406 - 4914 0.639 Yes 4961 0.443 - 5017 0.439 - 5080 0.459 - 5138 0.047 - 5182 0.155 -

Explain the output -- Processing locus 7: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATCATTGTAGCAGCAAAATTGACAGGATCATGCAAAGAAAAGAAGCGGAAAAAGCAAGAGAAGCTAAAGAATTACTCTG GAATTGGCGAGTGAAGAATGAGAGACGAGATTACTAGTAGAGCGATTGTCTCTGGCGCGGAAGCAGTCGAGAAAACAAGC CATGGCTCTACAGACTGATAAATCGATGAATCTGAGCACGGATCTGCACAAGGGGCTTAGGTTTCGGTTCATCAACAGTC GTCTCATATCATTTGATTTCCTCAAGAGCGAAACAGTGATTGACCAAGAAAACAATCAGGCTGACGATTTTCCCGAGAAA CACGCAGAAAATGAGAGAGAAAATGCCGTTGTTTTGAAATTTGAAACGAAAGGGTGCGAGGGAATGAATCAGGTGCGGTG CAGGTCTTCCCGATCACTGTTTTCTGTTAATTTCCTTCTGGCCCAAAAGGCCCAAAAAGCCCAAAAGACTCCAAGTTATT CAATAAGGAGTTTTTTTTTTCTTTTCTTAAAAAAATGTTTGTGGAAAAAAGGGAAAGTAGGGCAAGGAGGGAGGCGGAGA GCAAAACCGGAACACTCTCATCAACGAGCGTCTCTCTGGAAGCTCACATTACCGCCACATAGTCTCTGCTCACAAGTATT GAGAGAAAAGAAACCTTTGAAAGCGATGGCGAAGAAGAAGAAGGATACGAAGCATACTCGTCTCTGCGAAGCCACTGGGG CTTGGGCCACCAAGGTCCTTGAGGATTTTCGCGCCTCCGGGAACGATTCTTACGTCTTTGAGCAGCAACTTACAAATAGC GAACGTGGCATTATTCATCAGATGTGCCGAACTATGGGTCTCCGATCAAAAAGTAACGGGAGTGGGGAAGAAAGGCGTCT TTCTCTTTTCAAAGGAGATGGCATTTCAAAGTCAGACAAGAGGCGTATGTATGAAGCCAGAAATCAGAAAGAAAAAGAAG GAGATGGGATTTCAAAGTCATACAGCAAGCATCGCTATGAAACCAGATTTCAGAAAGCAGGCGGTATAAGAAAGACGAGA ATATCTCCAAAGAAGCTCAAATGTGTGTCATTTCCACCAGAGGCTAAAGCTGTTCTGCACGACTTATTTACTCGCTATCC ACCTTGTGACGGCGATACTACAGGGACATCACTGGGTATATATACTACGGGAAACGTCAACAGTAATTGGAAAGATGATT TCTTCAAGAAACCTCATATGACAAAACACGATATTGAAAATAACGTGGTATCTCTGAGTTCTAGACTGAAAAAAGAAAGA CATTTTCGAGAGATCTTTGAAGCACGATCAAAGCTTCCCATCGCATCTTTTCGCGATGCCATTATATCTGCAGTGGAATC CAATCAGGTTGTTCTTATTGCTGGTGAAACTGGTTGTGGGAAAACTACTCAGGTGCCTCAATATCTTCTAGATCATATGT GGCATAGTAAGAAAGAAGCATGCAAAATCATTTGCACCCAACCACGTCGTATCTCAGCTATTTCAGTTTCTGATCGAATA TCTTGGGAAAGAGGTGAAACTATTGGGAGAACTGTCGGCTACAAGGTTCGGCTGCAAAGTGAAGGTGGAAGAGAGTCATC AGTTGTTTTCTGCACGAATGGCATTCTATTGAGGGTGCTTATAGGCAAAGGCGTTAACAGCTCTGTTCCTGATATAACTC ACATTATTGTGGACGAAATCCATGAAAGGGACTCCTACTCTGATTTCATGTTAATGATTTTAAGGGACTTGCTTCCGTCT AATCCTCATCTCCGACTGATTCTGATGAGTGCTACTCTTGATGCTGAGAGGTTTTCTGAATATTTTGGAGGTTGTCCAGT TGTCCGAGTACCTGGATTCACTTATCCAGTGAGAACCTTTTTTCTGGATGATGCGCTCTCTGTCCTGAATTCAGACAAGA ATAGTCATCTACTTTCTGCTGTCAAACGTGATTTTAAAGATGAAGATAAAGTTTCTTTGGATGAAGCAATCGACTTGGCA TGGACAAATGATGAGTTTGATTGTCTCGTAGATCTAGTTTCATCTGAAGGAAGTCATGAGGCTTACAATTACCAGAACTC AACGACAGGCCTAACACCACTTATGGTCTTTGCTGGAAAGGGCAGGGTTAGCGATGTCTGTAAGCTCCTTTCAGTCGGCG CTGACTGTACACTGAAGTCCAAAGAAGGTATAACAGCATTAGAATTAGCTGAAAAGGAGAATCAATTTGAAACTGCTCAA ATAATCAGGGAACATGCGGGTAACATCCAGTCCAATTCTCAGCAAGCACAAGACTTACTTGATAAGTATATGGCGACAAT CAAACCAGAAGAAGTAGATGTGGGTCTTATAGTGAAGTTAATGAAGAAAATATGCAGCGATTCAAAAGATGGTGCCATTC TTGTTTTTCTACCTGGGTGGGAGGAAATAAGCAAAACAAAAGAGAAATTGCTTGATGATCGGTTTTTTGCACATAGTGCT AAATTCATCATTCTATGTCTTCACTCAAGGGTTCCAGCTGAGGAACAGAAGAAAGTTTTTAATCGCCCCCCTCGTGGTTG TCGCAAAATTGTACTTGCAACAAATATTGCTGAATCAGCAGTTACCATCGATGATGTGGTTTATGTGATAGATAGTGGTC GGATGAAGGAAAAGAGTTATGATCCCTATAATGACGTGTCAACGCTCCAATCTTCTTGGGTATCAAAAGCAAATGCAAAA CAGAGGGCAGGCCGAGCAGGCCGTTGCCAAGCTGGCATCTGTTATCATCTATATTCTAAACTCCGGGCAGCGTCTTTGCC CGAATATAGAGTTCCTGAAGTTATGAGAATGCCAGTGGATGAACTCTGCTTGCAGGTTAAGATGCTGGATCCGAATTGCA ACGTAAATGATTTCCTTCAGAAATTGATGGACCCGCCTGTTGCTCAAAGTATTGAAAATGCATTAATCATCCTTAAAGAT ATTGGAGCTTTGACTCCTGAAGAAGAGCTGACTGAACTTGGACAGAAATTTGGTCAGCTTCCAGTTCATCCTCGGATTAG CAAGATGATTTATTTCGCCATATTAGTGAACTGTTTGGATCCAGCACTTATTCTGGCATGTGCAGCTGACGAGAAGGATC CGTTTACCATGCCCTTGTCGCCAGGTGATAGAAAAAAGGCTGCTGCCGCAAAACATGAACTTGCATCGCTTTATGGAGAC CACAGTGATCATCTTGCCACTGTCGCGGCTTTTCAGTGCTGGAAAAATGCAAAAGCAAGTGGTCAAGCTAAGGAGTTTTG TTCTAAGTACTTCATCTCCCAAGTTGTCATGAAGAGGCTGGATGATCTGTGTCGCAAGCTTCAGGGAGAACTTAATAGAC ATGGGGTCATACCTAGCAGCAGCTCAAACTGTAGCCTGAATGCTCATGATCCGGGTATTCTCCGTGCTGTTATAGCAGTT GGATTGTACCCTATGTTGGGAAGGATGTGCCCACTTTCCAAGAATCGGACAAGGTCTGTAATAGAGACTATTGCTGGAGC CAAAGTCCGTGTGCCCTCGCTATCAAACAATGTTGACATGTCCTCTACCAAGTTTGACGAAGCTTTAATTGTTTTTGATG AGATCACTCGAGGAGATTGGGGTGTGGTGATAAGGAGCTGCACTGTTCTTCCCACTATACCTGTGTTACTGTTTTCAAGA GAGATAGCCGTTTCTACTACAGAGAGCTATGATGCTGTTAAGAGTGATGACGAGGAAGACCACAAAGTAGGAAATGTAGG AGACGCGATGGACATAGATAAAGAAGTTGGTAGGCCTGGAGAGAAAATAATGCTTGGGCCGGAAAATTCAGTAAAAGTAG TAGTGGATCGGTGGCTGCCTTTCAAGGTTACAGCTTTTGAAATTGCTCAAATGTACATCTTGAGAGAGAGACTAATGGCG TCCATTTTGTTCAAGGTTAAACATCCAAAGGAAAATTTGCCGCCTCATCTCGGTGCTTCTATGTATGCAATAGCTTCTGT TCTCTCATATGATAGCCTCGCACAATCTTCAGTTCAAACTGTAGCTGTGCAACCTATTACTTCAGTGGTAGATGCAACGA GTCCCAGAGATGACATACCTTCCACTAACCCCAATGAGCTTCGGGAGCATGACCCGAACACTACTCCAATGGGGTCAAAG CTCGAATTAGCCAACAAGTTGGGTTTGGGTAATATGGAAGAGAGCTTGCCTTCAAATTTCGCTGATGGGAATGAGCAACC TGACCCAAACACTTCTCCAGTGGAAGATGTTTCGGCGGCTACAAAACAAAAGAAGATGCAGTCAGAGTCCAAAAGGTGCA AGTCGCTCAACAACGTGGATTTGGGGAACATTGAAGAGAATTTCGGGAACATGGAAGAGAACCCACCTTCTGATCTGGCT ATTGGGAATGAGCAAACATTGCCGAAGTTAGCTAGCAACTTGGACATGGGGAACATGGAAGAGAACACGCCTTCTGATCT GGCCAATGGGAATGAGAAAACAGAGCCTAACTCAGCCAACAGCATGGATTTAGGGAACATGGAAGAGAACACGCCTTCTG ATTTGGCAAATGGGAATAAGAAAAAAGAGCCTAAGTCAGTCAGCAAGTTGGATCTGGGTAGCGAGAAAGTGAGCATTCCT TCTAATCTCGTCAATGGAAATGAGCAACATGACCTAAACATTGCTCCAGGTGAAGATGCTTCAGCGGCTAAGCAACCAGA AAAGAAGCGATCCAGGTCTAAAAAGCGCAAATCAGGCAACAACCTGGATTTGGGGAAGATGGAAAAGAGCAAGCCTTCCG ATCTGGCAAATGAGAATGAGCAAACAGAGCCCAAGTCAGCCAACAACTTGGATTTAGGGAATATGAAAGAGAACACGCCT TCGGATCTGGCTAATGAGAATGAGCAAACAGAGCTTAGGCTACCTAACAACTCGGATTATGGTAACATGGAAGAGAGCTT GCCTTTGAATCTTGCTAATGGGGATGAACAACCTGACCCAACCACTGCTCCAATGGAAGCTGCTAAGCAACCAAAAAAGA AGCGATCAAGGTCCAAAAAGTGCAAATCAGTCAACAACCTGGACTTGGGGAACATGGAAGAAAACAAGCCTTCTGATCTG GCAAACGGAAATGAGCAAAAAGATCCTGAGTCAGTCAACAGGTTGGATCCGGGTAAGGAGAAAGAGAGCATTCCTTCGAA TCTTGTCAGTGGGAATGAGCAACCTGACTCAAACACTGCTCCAGCTAAGAAACCAAAAAAGAAAAAGCGCAAATTAGCTA ACAACTTTGATTCGGTTAACAATATGGAAGAGAAAATGCCTTCCACTAATGTATTGAGCCAGGGAAACAAGTCAGGTTTG ATTGAGGAAAAACCGAGCATACCTTCCGATCAATGAGCAGTGTGGCTCAAATACTGCACCAACCTAAGCAAAACCGAAAG AAGAAGGGAAGTATGATGCCCATTCCACTTTGGTGTTTTAATGAGCATTTCGGTG ..............................N................................................. .................i.............................................................. ................................................................................ ..........N...........i........................................N................ ................................................................................ ..................................i............................................. ................................................................................ .........................i...................................................... ................................................................................ .....................N...........i.............................................. .................i............................N...i............................. ...i................................N........................................... ....................N........................................................... ..........................................................................N..... .................i.............................................................. .......................................................N........................ ............................................................................N... ...................N............................................................ ................................................................................ .................N.............................................................. .....................N.........................N.....N.......................... ........................N...............N....................................... ...............................................N..N............................. .......................................N....................N..................N .......N..N............................................i........................ ......................i..............................i.......................... ................................................................................ .............N.......................................................N.......... .................N......................N..........N................N........... ......................................................N......................... ..............N................................................................. ..................................................N..N........N................. ..i...............N...........N.........................................N....... ................................................................................ ......................i.....i.........N......................N.................. ......N...................N..............................N...................... ................................................................................ ....i....................................................N...................... ........i.............................................N.................N....... ..............................................N................................. ............................i............N...................................... N......................................N.....N.................................. ............N...........i....................................................... .............................N.......N.......................................N.. ................................................................................ ............................N..N..............N..........................N...... .......i.........................................i.............................. ..................................................N.......................i..... ............................................................N...N............... ........N..............................................................N........ .........N.......................N..............N...................i........... .................................i..............................N.....N......... ..........................N............................i........................ ..................................................i............................. .......N.....................................i........i......................... .....N.....N...............................i..............i..................... .........N...................................................................... .............N.....N........N..........................N........................ ..........................................................i..................... .........N.....N..............................................i................. .............N.....N......................................N.......i............. .................i.....N............................i........................... .....................................................i.......................... ..........N..................................................................... ..............N................................................................. .......................i...........N............N............................... ................................N............................................... ............N..N........................N.............. Pos Score Pred ------------------------ 31 0.466 - 98 0.779 Yes 162 0.283 - 186 0.278 - 331 0.192 - 343 0.630 Yes 384 0.459 - 515 0.591 Yes 666 0.795 Yes 822 0.489 - 834 0.626 Yes 898 0.569 Yes 927 0.372 - 931 0.505 Yes 964 0.552 Yes 997 0.140 - 1061 0.233 - 1195 0.291 - 1218 0.538 Yes 1336 0.306 - 1437 0.372 - 1460 0.400 - 1618 0.275 - 1702 0.181 - 1728 0.224 - 1734 0.358 - 1785 0.481 - 1801 0.164 - 1888 0.142 - 1891 0.255 - 1960 0.245 - 1981 0.189 - 2000 0.258 - 2008 0.321 - 2011 0.109 - 2056 0.526 Yes 2103 0.674 Yes 2134 0.659 Yes 2254 0.195 - 2310 0.263 - 2338 0.381 - 2361 0.305 - 2372 0.436 - 2389 0.268 - 2455 0.230 - 2495 0.152 - 2611 0.225 - 2614 0.101 - 2623 0.109 - 2643 0.728 Yes 2659 0.338 - 2671 0.461 - 2713 0.451 - 2823 0.657 Yes 2829 0.770 Yes 2839 0.312 - 2862 0.276 - 2887 0.101 - 2907 0.332 - 2938 0.134 - 3045 0.510 Yes 3098 0.161 - 3129 0.816 Yes 3175 0.388 - 3193 0.342 - 3247 0.374 - 3309 0.787 Yes 3322 0.411 - 3361 0.318 - 3400 0.209 - 3406 0.158 - 3453 0.472 - 3465 0.535 Yes 3550 0.164 - 3558 0.481 - 3598 0.118 - 3709 0.214 - 3712 0.153 - 3727 0.324 - 3754 0.206 - 3768 0.633 Yes 3810 0.616 Yes 3891 0.157 - 3915 0.587 Yes 3981 0.486 - 3985 0.104 - 4009 0.293 - 4072 0.306 - 4090 0.464 - 4114 0.279 - 4129 0.229 - 4149 0.601 Yes 4194 0.809 Yes 4225 0.442 - 4231 0.167 - 4267 0.154 - 4296 0.768 Yes 4371 0.632 Yes 4408 0.107 - 4446 0.828 Yes 4455 0.776 Yes 4486 0.376 - 4492 0.215 - 4524 0.679 Yes 4539 0.687 Yes 4570 0.403 - 4654 0.380 - 4660 0.403 - 4669 0.433 - 4696 0.233 - 4779 0.815 Yes 4810 0.239 - 4816 0.248 - 4863 0.654 Yes 4894 0.357 - 4900 0.300 - 4939 0.198 - 4947 0.749 Yes 4978 0.572 Yes 4984 0.236 - 5013 0.584 Yes 5094 0.855 Yes 5131 0.449 - 5215 0.380 - 5304 0.607 Yes 5316 0.443 - 5329 0.376 - 5393 0.290 - 5453 0.047 - 5456 0.089 - 5481 0.035 -

Explain the output -- Processing locus 8: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACTCGGAAATTTTCCTGGGCTTTGTTATTATTTGCGTCGGTTTAGTCAATGCGATGTAGTGAACGCTTCGATTACATAG ATTTGTGTGAAAATTTTATAGTCCAATTGCGTTATAGTGATGATTTTGTTGGTAATTTTGATTTTGAAACTAGGGATTTT GAAAATGGTGGAAAATGGGGCTAAAGCTGCGAAGCGAAAGAAGAGACCACTTCCAGAGATTCAAGAGGTAGAAGATGTAC CTAGGACGAGGAGACCAAGGCGTGCTGCAGCGTGTACCAGTTTCAAGGAGAAATCTATTCGAGTCTGTGAGAAATCTGCT ACTATTGAAGTAAAGAAACAGCAGATTGTGGAGGAAGAGTTTCTCGCGTTACGGTTAACGGCTCTGGAAACTGATGTTGA AGATCGTCCAACCAGGAGACTGAATGATTTTGTTTTGTTTGATTCAGATGGAGTTCCACAACCTCTGGAGATGTTGGAGA TTCATGACATATTCGTTTCAGGTGCTATCTTACCTTCAGATGTGTGTACTGATAAGGAGAAAGAGAAGGGTGTGAGGTGT ACATCGTTTGGACGGGTTGAGCATTGGAGTATCTCTGGTTATGAAGATGGTTCCCCTGTTATTTGGATCTCAACGGAATT GGCGGATTATGATTGTCGTAAACCTGCTGCTAGCTACAGGAAGGTTTATGATTACTTCTATGAGAAAGCTCGTGCTTCAG TGGCTGTGTATAAGAAATTGTCCAAGTCATCTGGTGGGGATCCTGATATAGGTCTTGAGGAGTTACTTGCGGCGGTTGTC AGATCAATGAGCAGTGGAAGCAAGTACTTTTCTAGTGGTGCGGCAATCATCGATTTTGTTATATCCCAGGGAGATTTTAT ATATAACCAACTCGCTGGTTTGGATGAGACAGCCAAGAAACATGAATCAAGCTATGTTGAGATTCCTGTTCTTGTAGCTC TCAGAGAGAAGAGTAGTAAGATTGACAAGCCTCTGCAGAGGGAAAGAAACCCATCTAATGGTGTGAGGATTAAAGAAGTT TCTCAAGTTGCGGAGAGCGAGGCCTTGACATCTGATCAACTGGTTGATGGTACTGATGATGACAGAAGATATGCTATACT CTTACAAGACGAAGAGAATAGGAAATCTATGCAACAGCCCAGAAAAAACAGCAGCTCAGGTTCTGCTTCAAATATGTTCT ACATTAAGATAAATGAAGATGAGATTGCCAATGATTATCCTCTCCCATCGTACTATAAGACCTCCGAAGAAGAAACAGAT GAACTTATACTTTATGATGCTTCCTATGAGGTTCAATCTGAACACCTGCCTCACAGGATGCTTCACAACTGGGCTCTTTA TAACTCTGATTTACGATTCATATCACTGGAACTTCTACCGATGAAACAATGTGATGATATTGATGTCAACATTTTTGGGT CAGGTGTGGTGACTGATGATAATGGAAGTTGGATTTCTTTAAACGATCCTGACAGCGGTTCTCAGTCACACGATCCTGAT GGGATGTGCATATTCCTCAGTCAAATTAAAGAATGGATGATTGAGTTTGGGAGCGATGATATTATCTCCATTTCTATACG AACAGATGTGGCCTGGTACCGTCTTGGGAAACCATCAAAACTTTATGCCCCTTGGTGGAAACCTGTTCTGAAAACAGCAA GGGTTGGGATAAGCATTCTTACTTTTCTTAGGGTGGAAAGTAGGGTTGCTAGGCTTTCATTTGCAGATGTCACAAAAAGA CTGTCTGGGTTACAGGCGAATGATAAAGCTTACATTTCTTCTGACCCCTTGGCTGTTGAGAGATATTTGGTCGTCCATGG GCAAATTATTTTACAGCTTTTTGCAGTTTATCCGGACGACAATGTCAAAAGGTGTCCATTTGTTGTTGGTCTTGCAAGCA AATTGGAGGATAGGCACCACACAAAATGGATCATCAAGAAGAAGAAAATTTCGCTGAAGGAACTGAATCTGAATCCAAGG GCAGGCATGGCACCAGTAGCATCGAAGAGGAAAGCTATGCAAGCAACAACAACTCGCCTGGTCAACAGAATTTGGGGAGA GTTTTACTCCAATTACTCTCCAGAGGATCCATTGCAGGCGACTGCTGCAGAAAATGGGGAGGATGAGGTGGAAGAGGAAG GCGGAAATGGGGAGGAAGAGGTTGAAGAGGAAGGTGAAAATGGTCTCACAGAGGACACTGTACCAGAACCTGTTGAGGTT CAGAAGCCTCATACTCCTAAGAAAATCCGAGGCAGTTCTGGAAAAAGGGAAATAAAATGGGATGGTGAGAGTCTAGGAAA AACTTCTGCTGGCGAGCCTCTCTATCAACAAGCCCTTGTTGGAGGGGAAATGGTGGCTGTAGGTGGCGCTGTCACCTTGG AAGTTGATGATCCAGATGAAATGCCGGCCATCTATTTTGTGGAGTACATGTTCGAAAGTACAGATCACTGCAAAATGTTA CATGGTAGATTCTTACAAAGAGGATCTATGACTGTTCTGGGGAATGCTGCTAACGAGAGGGAACTATTCCTGACTAATGA ATGCATGACTACACAGCTCAAGGACATTAAAGGAGTAGCCAGTTTTGAGATTCGATCAAGGCCATGGGGGCATCAGTATA GGAAAAAGAACATCACTGCGGATAAGCTTGACTGGGCTAGAGCATTAGAAAGAAAAGTAAAAGATTTGCCAACAGAGTAT TACTGCAAAAGCTTGTACTCACCTGAGAGAGGGGGATTCTTTAGTCTTCCACTAAGTGATATTGGTCGCAGTTCTGGGTT CTGCACTTCATGTAAGATAAGGGAGGATGAAGAGAAGAGGTCTACAATTAAACTAAATGTTTCAAAGACAGGCTTTTTCA TCAATGGGATTGAGTATTCTGTTGAGGATTTTGTCTATGTCAACCCTGACTCTATTGGTGGGTTGAAGGAGGGTAGTAAA ACTTCTTTTAAGTCTGGGCGAAACATTGGGTTAAGAGCGTATGTTGTTTGCCAATTGCTGGAAATTGTTCCAAAGGAATC TAGAAAGGCTGATTTGGGTTCCTTTGATGTTAAAGTGAGAAGGTTTTATAGGCCTGAGGATGTTTCTGCAGAGAAGGCCT ATGCTTCAGACATCCAAGAATTGTATTTCAGCCAGGACACAGTTGTTCTCCCTCCAGGTGCTCTAGAGGGAAAATGTGAA GTAAGAAAGAAAAGTGATATGCCCTTATCCCGTGAATATCCAATATCAGACCATATTTTCTTCTGTGATCTTTTCTTTGA CACCTCCAAAGGTTCTCTCAAGCAGCTGCCCGCCAATATGAAGCCAAAGTTCTCTACTATTAAGGACGACACACTTTTAA GAAAGAAAAAGGGAAAGGGAGTAGAGAGTGAAATTGAGTCTGAGATTGTCAAGCCTGTTGAGCCACCTAAAGAGATTCGT CTGGCTACTCTAGATATTTTTGCTGGTTGTGGTGGCCTGTCTCATGGACTGAAAAAGGCGGGTGTATCTGATGCAAAGTG GGCGATTGAGTATGAAGAGCCAGCTGGGCAGGCTTTTAAACAAAACCATCCTGAGTCAACAGTTTTTGTTGACAACTGCA ATGTGATTCTTAGGGCTATAATGGAGAAAGGTGGAGATCAAGATGATTGTGTCTCTACTACAGAGGCAAATGAATTAGCA GCTAAACTAACTGAGGAGCAGAAGAGTACTCTGCCACTGCCTGGTCAAGTGGACTTCATCAATGGTGGACCTCCATGTCA GGGATTTTCTGGTATGAACAGGTTCAACCAAAGCTCTTGGAGTAAAGTTCAGTGTGAAATGATATTAGCATTCTTGTCCT TTGCTGACTATTTCCGGCCAAGGTATTTTCTTCTGGAGAACGTGAGGACCTTTGTGTCATTCAATAAAGGGCAGACATTT CAGCTTACTTTGGCTTCCCTTCTCGAAATGGGTTACCAGGTGAGATTTGGAATCCTGGAGGCCGGTGCATATGGAGTATC CCAATCTCGTAAACGAGCTTTCATTTGGGCTGCTGCACCAGAAGAAGTTCTCCCTGAATGGCCTGAGCCGATGCATGTCT TTGGTGTTCCAAAGTTGAAAATCTCACTATCTCAAGGTTTACATTATGCTGCTGTTCGTAGTACTGCACTTGGTGCCCCT TTCCGTCCAATCACCGTGAGAGACACAATTGGTGATCTTCCATCAGTAGAAAACGGAGACTCTAGGACAAACAAAGAGTA TAAAGAGGTTGCAGTCTCGTGGTTCCAAAAGGAGATAAGAGGAAACACGATTGCTCTCACTGATCATATCTGCAAGGCTA TGAATGAGCTTAACCTCATTCGATGCAAATTAATCCCAACTAGGCCTGGGGCTGATTGGCATGACTTGCCAAAGAGAAAG GTTACGTTATCTGATGGGCGCGTAGAAGAAATGATTCCTTTTTGTCTCCCAAACACAGCTGAGCGCCACAACGGTTGGAA GGGACTATATGGGAGATTAGATTGGCAAGGAAACTTTCCGACTTCCGTCACGGATCCTCAGCCCATGGGTAAGGTTGGAA TGTGCTTTCATCCTGAACAGCACAGAATCCTTACAGTCCGTGAATGCGCCCGATCTCAGGGGTTTCCGGATAGCTACGAG TTTGCAGGGAACATAAATCACAAGCACAGGCAGATTGGGAATGCAGTCCCTCCACCATTGGCATTTGCTCTAGGTCGTAA GCTCAAAGAAGCCCTACATCTCAAGAAGTCTCCTCAACACCAACCCTAGATAACCACCCAAATTTGGCATTTCCTTTTTC AATAATATTAGTCATTATGATCCTTGTCTTGAATGAAACTCATTGGTGCTGATACTTTTGATAAAGAA .................................................N....N......................... .......................................N........................................ ....i.........N...........................................................N..... ................................................................................ .........................................................................N...... .......................N.......................N......................i......... ...N...................................N........................................ ........................................N.....N................................. ........N......................................N...........N.................... ................................................................................ ......i......................................................................... .......................N.................N...........N.......................... .........................................................i...................... ..............................................N........N..N...........N......... ............................i............................................N...... ............N.....N...........N...............................................i. .............N..N........N...............................N...................... ........................................i.......N....N........i................. ...............N.....N........................................................N. ...N............................i...i..................N........................ .....N......................................N................................... ..................................................................N............. ...................N........................................................N... .........................................N...................................... .........................i...................................................... ......i.............................i........................................... .....................................................N........N................. ......N................................N........................................ ........................................................i....i.................. .................................................i.............................. ......N........N....i..........................N..........................i..... i...N..........................................................N................ ................................................................................ ................................................................................ .........N................N.............................N....................... ...N................................N........................................... ........................................N....................................... ..........................N................................N.................... N........................................................................N...... ..................i............................................................. .....................................i.......................................... ................................................................................ ...........................................N..........................N......... ...........N.................................................................... N...................i.....................N..........................N.......... .............................................................i............N..... .............N............................................N..................... ................................................................................ ...........................i..........................................N......... .........................................................i............i...N..... .............................................N.................................. ................................................................................ ...............................................................................i ...N..................N.....................................i................... .............i................N................................................. ........N.......................................................i..............N ...........................................N.................................... ........................................N....................................... ................................................................................ ................N...............N................................... Pos Score Pred ------------------------ 50 0.395 - 55 0.216 - 120 0.334 - 165 0.860 Yes 175 0.360 - 235 0.222 - 394 0.142 - 424 0.065 - 448 0.171 - 471 0.778 Yes 484 0.122 - 520 0.348 - 601 0.150 - 607 0.305 - 649 0.229 - 688 0.117 - 700 0.097 - 807 0.666 Yes 904 0.246 - 922 0.345 - 934 0.314 - 1018 0.585 Yes 1087 0.076 - 1096 0.189 - 1099 0.438 - 1111 0.343 - 1149 0.522 Yes 1194 0.420 - 1213 0.243 - 1219 0.305 - 1231 0.232 - 1279 0.513 Yes 1294 0.158 - 1297 0.254 - 1306 0.245 - 1338 0.419 - 1401 0.700 Yes 1409 0.375 - 1414 0.273 - 1423 0.622 Yes 1456 0.257 - 1462 0.356 - 1519 0.241 - 1524 0.406 - 1553 0.572 Yes 1557 0.511 Yes 1576 0.315 - 1606 0.261 - 1645 0.165 - 1747 0.140 - 1780 0.249 - 1837 0.332 - 1882 0.199 - 1946 0.901 Yes 2007 0.508 Yes 2037 0.519 Yes 2134 0.220 - 2143 0.300 - 2167 0.382 - 2200 0.322 - 2297 0.547 Yes 2302 0.517 Yes 2370 0.801 Yes 2407 0.136 - 2416 0.109 - 2421 0.718 Yes 2448 0.179 - 2475 0.551 Yes 2482 0.347 - 2508 0.787 Yes 2524 0.322 - 2557 0.399 - 2561 0.687 Yes 2565 0.441 - 2624 0.263 - 2810 0.078 - 2827 0.176 - 2857 0.181 - 2884 0.237 - 2917 0.267 - 3001 0.253 - 3067 0.300 - 3100 0.309 - 3121 0.115 - 3194 0.208 - 3219 0.532 Yes 3318 0.672 Yes 3484 0.199 - 3511 0.076 - 3532 0.342 - 3601 0.179 - 3621 0.717 Yes 3643 0.137 - 3670 0.248 - 3742 0.537 Yes 3755 0.238 - 3774 0.164 - 3819 0.316 - 3948 0.520 Yes 3991 0.326 - 4058 0.559 Yes 4071 0.511 Yes 4075 0.252 - 4126 0.304 - 4320 0.505 Yes 4324 0.210 - 4343 0.346 - 4381 0.558 Yes 4414 0.523 Yes 4431 0.215 - 4489 0.462 - 4545 0.635 Yes 4560 0.357 - 4604 0.446 - 4681 0.335 - 4817 0.045 - 4833 0.047 -

Explain the output -- Processing locus 9: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTGTACGAACTATCTCTTGATATAGTTGTAAAGGTTTTAGATTGGGAACAAAAAATTTCACTGTGATGTTTGTTTAGG TAGTAGAGTTCAATTGTGTGAAACTCTAATTTCATAGTTTGCTTAAGTCTCAGAACTAGCACTGAGTCTATTGTTATATG TGTTGTTTATGTAAGTTTGTTGTTATTGATGGGAGATATGTTTTGTGTGCAGAAAATGAGTAGTTTGGTGGAGAGGCTTC GCATACGATCTGATAGGAAACCAGTTTATAACCTAGATGATTCTGATGATGACGACTTCGTTCCTAAAAAAGATCGAACC TTTGAGCAAGTCGAGGCTATTGTCAGAACTGATGCGAAAGAAAATGCATGTCAGGCTTGTGGGGAAAGTACTAATCTTGT AAGCTGCAATACATGCACTTATGCGTTCCATGCTAAATGCTTAGTTCCACCTCTTAAAGATGCTTCCGTGGAAAATTGGA GATGCCCTGAATGTGTTAGTCCTCTTAACGAGATAGATAAGATATTGGATTGTGAAATGCGTCCTACAAAATCTAGTGAA CAAGGTTCCTCCGATGCGGAACCGAAGCCAATTTTTGTGAAACAGTATCTCGTGAAGTGGAAGGGATTATCATACCTTCA CTGCTCTTGGGTGCCTGAGAAGGAGTTCCAGAAGGCTTATAAGTCAAATCATCGTTTAAAAACCAGAGTGAACAATTTTC ACCGTCAAATGGAGTCATTCAATAACAGCGAAGATGATTTTGTTGCCATACGTCCTGAGTGGACCACTGTTGATCGGATT CTTGCCTGCAGAGAGGAAGATGGGGAGCTGGAATATCTTGTCAAATATAAAGAGCTATCCTATGATGAATGTTATTGGGA GTCAGAATCAGACATCTCAACCTTCCAGAATGAAATTCAAAGGTTCAAGGATGTAAATTCTAGAACTCGCAGAAGTAAAG ATGTTGACCATAAAAGAAATCCCAGAGACTTTCAACAGTTTGATCATACTCCTGAATTCCTCAAAGGCTTGTTACATCCA TACCAGCTTGAGGGACTTAATTTTTTGCGGTTCTCGTGGTCAAAACAGACGCATGTAATCCTTGCTGATGAAATGGGACT AGGCAAGACAATTCAAAGCATTGCCCTTTTAGCTTCACTTTTTGAGGAGAACCTCATTCCGCATTTGGTAATTGCTCCTC TATCGACTCTGCGTAACTGGGAGAGAGAGTTTGCCACATGGGCCCCACAGATGAACGTGGTTATGTATTTTGGCACTGCG CAAGCTCGAGCAGTTATCAGAGAACATGAGTTTTACTTATCGAAAGATCAAAAAAAGATCAAGAAAAAGAAATCTGGACA AATAAGTAGCGAAAGCAAGCAAAAAAGAATCAAGTTTGATGTCCTCCTCACATCGTATGAGATGATCAACCTAGATTCAG CAGTTCTAAAACCAATTAAGTGGGAGTGCATGATTGTTGATGAAGGTCATCGACTGAAAAATAAGGATTCAAAGCTGTTC TCTTCATTGACACAGTATTCAAGTAACCACCGTATTCTTCTGACAGGAACACCACTTCAGAACAACTTGGATGAACTTTT CATGCTCATGCATTTTCTTGATGCGGGGAAGTTTGGAAGTTTGGAGGAGTTCCAGGAGGAGTTCAAAGATATTAATCAAG AGGAGCAGATCTCAAGGTTGCACAAAATGTTGGCTCCACATTTGCTCAGAAGGGTAAAAAAAGACGTAATGAAAGACATG CCCCCCAAAAAGGAGCTCATTTTGCGTGTTGATCTGAGCAGTCTGCAGAAAGAATATTACAAAGCTATTTTTACCCGTAA TTATCAAGTATTGACAAAAAAGGGAGGTGCTCAAATTTCCCTTAATAACATTATGATGGAATTACGAAAAGTATGCTGCC ATCCTTATATGCTAGAGGGTGTTGAGCCAGTTATTCACGACGCAAATGAAGCTTTCAAACAACTTTTGGAGTCTTGTGGA AAGCTGCAACTTCTAGATAAAATGATGGTCAAACTGAAAGAGCAAGGACACAGAGTCCTAATATACACACAGTTTCAGCA TATGCTGGACTTACTTGAAGACTACTGTACCCATAAGAAATGGCAGTACGAGCGAATTGATGGAAAGGTTGGCGGAGCTG AGCGGCAAATACGCATAGATCGGTTCAATGCCAAAAATTCTAACAAGTTTTGTTTTTTGCTCTCCACAAGAGCTGGTGGC TTAGGAATAAATCTTGCAACGGCTGATACAGTAATCATTTATGACAGTGACTGGAATCCTCATGCTGATCTTCAAGCAAT GGCTAGAGCTCATCGACTTGGCCAAACAAATAAGGTGATGATTTATAGGCTCATAAACCGAGGCACCATTGAAGAAAGGA TGATGCAATTGACTAAAAAGAAAATGGTTCTAGAGCATCTTGTTGTTGGGAAACTCAAAACACAAAACATTAATCAGGAA GAGTTAGATGACATCATCAGGTATGGATCAAAGGAGCTTTTTGCTAGTGAAGATGATGAAGCAGGAAAGTCTGGAAAAAT TCATTATGATGATGCGGCTATAGACAAATTGCTTGATCGTGATCTCGTGGAGGCAGAGGAAGTCTCAGTGGATGATGAAG AGGAGAATGGATTCTTAAAGGCTTTCAAGGTGGCTAATTTTGAATATATAGATGAAAATGAGGCAGCAGCATTAGAGGCA CAGAGAGTCGCTGCTGAAAGCAAATCTTCAGCAGGCAATTCTGATAGAGCAAGTTATTGGGAAGAGTTGTTAAAAGATAA ATTTGAGCTGCACCAGGCTGAGGAGCTTAATGCTCTTGGAAAAAGGAAGAGAAGTCGCAAGCAGTTGGTATCCATTGAAG AAGATGATCTTGCTGGTTTGGAAGATGTGAGCTCTGATGGAGATGAAAGTTATGAAGCTGAGTCAACAGATGGTGAAGCA GCAGGACAAGGAGTTCAGACGGGTCGACGGCCGTACAGAAGAAAGGGTCGCGATAATTTGGAACCAACTCCGTTGATGGA AGGTGAGGGGAGATCTTTCAGAGTACTGGGTTTCAACCAGAGTCAAAGGGCCATTTTTGTACAGACTTTGATGAGGTATG GAGCTGGCAATTTTGATTGGAAGGAGTTTGTTCCTCGCTTAAAGCAGAAGACCTTTGAAGAAATAAATGAATATGGAATA CTCTTCTTGAAGCACATTGCTGAAGAAATAGACGAGAATTCTCCAACCTTTTCAGATGGTGTGCCCAAGGAAGGACTTAG AATAGAAGATGTTCTAGTCAGAATTGCTCTTCTGATACTAGTTCAGGAGAAGGTGAAATTTGTAGAAGATCATCCAGGGA AACCTGTTTTCCCCTCTCGCATTCTTGAAAGATTCCCCGGACTGAGAAGTGGAAAAATTTGGAAGGAGGAACATGACAAG ATAATGATACGTGCTGTTTTAAAGCATGGGTACGGACGGTGGCAAGCTATTGTTGATGACAAAGAGTTGGGGATCCAAGA GCTTATCTGCAAAGAATTGAATTTCCCTCACATAAGTTTGTCTGCTGCTGAACAAGCTGGTTTGCAGGGGCAGAATGGTA GTGGGGGCTCTAATCCGGGAGCACAGACTAACCAGAATCCTGGAAGCGTTATTACTGGGAACAATAATGCTTCTGCTGAT GGGGCTCAAGTAAACTCGATGTTCTATTATCGGGACATGCAGAGACGACTTGTTGAGTTTGTGAAAAAGCGAGTTCTGCT TTTGGAGAAGGCGATGAATTATGAATACGCAGAGGAATATTATGGACTTGGTGGCTCATCATCTATCCCTACTGAAGAAC CAGAAGCTGAACCAAAGATCGCTGACACAGTGGGAGTGAGCTTTATTGAGGTTGATGATGAAATGCTTGATGGACTTCCT AAGACTGATCCTATCACTTCAGAAGAAATTATGGGGGCTGCTGTTGACAACAACCAAGCGCGGGTCGAAATAGCTCAACA TTATAACCAGATGTGCAAACTTCTTGATGAGAACGCTCGGGAATCAGTCCAAGCATATGTAAACAACCAACCACCGAGTA CCAAGGTGAATGAGAGCTTCCGTGCACTCAAATCTATCAATGGTAACATTAACACAATCCTTTCGATTACATCTGATCAA TCCAAGTCACATGAAGACGACACCAAGCCAGACCTAAACAATGTTGAGATGAAGGACACGGCCGAAGAAACAAAACCGTT AAGAGGTGGCGTCGTCGATCTGAATGTGGTGGAGGGAGAGGAGAACATTGCTGAAGCTAGTGGAAGTGTTGATGTAAAAA TGGAAGAAGCCAAAGAAGAAGAGAAGCCAAAGAACATGGTCGTTGATTGACTCAACTGGTAAATCAAGATTCAAGAGCCA AGGGGTCATGAAGTAGGAACTGTTGTATTATCTTTGGTTACATGTATGAGACTTATTCTTCCTTTTGTA ...................................................................N............ .............................................................................i.. ........N...................i........i.................i........................ ....................................N........N..N............................... ...............................i...........N...N................................ ............N.......N........N......N......................N.................... .............N.................................................................. ................................................................................ ........i........................i.............................................. ...................N.........................................N..N...N........... .............................i....................N............................. N............................................................................... ....................................................N..............N....N....... ................................................................................ .....................................N............i...........i................. .........................i...................................................... ......................................N.................N....N.................. .............................N.........N........................................ ......................................................................N......... ..........................i.........................................i........N.. ................................................................................ ....................................................N..i................N....... ........N....................................N.................................. .....................i..i....................................................... ...........................N.................................................... ........................................N....................N................i. .....................................N.........................................N ..N....................i........................................................ .......N..............N.............................N..N........................ .....N..N..N...........................................................N..N..... ......N............................................N.....N...................... ................................................................................ .............................N.................................................. ...N....................i...........N.....N........N.................N.......... ...........................................................................i.... ......................................................................N......N.. ..................................................................N.....i....... .......................................................N........................ ........N....................................................................... ........................................................................i....... ...N.....................N.............................N........................ ..........................................................................N..... ..................................................................N...........N. ..................N.................i........................................... .............i......N....................N...................................... ......................................................N..N....i......N.......... ..............................i................................................. ..........i...............N.............................N....................... .........i.............................N........................................ ..........N.............................N.......i............................... .......................N...............................................N.......i ...................................i............................................ .......N.................................N...N....................... Pos Score Pred ------------------------ 68 0.194 - 158 0.643 Yes 169 0.170 - 189 0.666 Yes 198 0.748 Yes 216 0.590 Yes 277 0.130 - 286 0.129 - 289 0.164 - 352 0.536 Yes 364 0.174 - 368 0.205 - 413 0.198 - 421 0.158 - 430 0.155 - 437 0.188 - 460 0.412 - 482 0.271 - 491 0.491 - 537 0.437 - 574 0.457 - 729 0.815 Yes 754 0.525 Yes 820 0.349 - 862 0.212 - 865 0.174 - 869 0.289 - 910 0.529 Yes 931 0.261 - 961 0.409 - 1093 0.153 - 1108 0.167 - 1113 0.402 - 1238 0.481 - 1251 0.660 Yes 1263 0.548 Yes 1306 0.532 Yes 1399 0.192 - 1417 0.214 - 1422 0.263 - 1470 0.368 - 1480 0.296 - 1591 0.181 - 1602 0.633 Yes 1608 0.241 - 1621 0.098 - 1707 0.561 Yes 1749 0.523 Yes 1758 0.419 - 1893 0.466 - 1896 0.631 Yes 1913 0.451 - 1929 0.227 - 1966 0.294 - 2022 0.589 Yes 2025 0.600 Yes 2082 0.574 Yes 2120 0.378 - 2140 0.351 - 2188 0.274 - 2281 0.406 - 2302 0.136 - 2319 0.746 Yes 2358 0.433 - 2400 0.462 - 2403 0.356 - 2424 0.744 Yes 2488 0.134 - 2503 0.342 - 2533 0.234 - 2536 0.209 - 2566 0.164 - 2569 0.130 - 2572 0.104 - 2632 0.227 - 2635 0.142 - 2647 0.199 - 2692 0.284 - 2698 0.402 - 2830 0.104 - 2884 0.272 - 2905 0.713 Yes 2917 0.497 - 2923 0.202 - 2932 0.404 - 2950 0.289 - 3036 0.508 Yes 3111 0.374 - 3118 0.261 - 3187 0.389 - 3193 0.516 Yes 3256 0.274 - 3289 0.161 - 3433 0.626 Yes 3444 0.472 - 3466 0.368 - 3496 0.057 - 3595 0.350 - 3667 0.111 - 3679 0.100 - 3699 0.403 - 3717 0.545 Yes 3774 0.593 Yes 3781 0.494 - 3802 0.448 - 3895 0.133 - 3898 0.171 - 3903 0.838 Yes 3910 0.242 - 3951 0.890 Yes 4011 0.616 Yes 4027 0.446 - 4057 0.103 - 4090 0.575 Yes 4120 0.465 - 4171 0.155 - 4201 0.221 - 4209 0.817 Yes 4264 0.149 - 4312 0.130 - 4320 0.676 Yes 4356 0.599 Yes 4408 0.035 - 4442 0.079 - 4446 0.045 -

Explain the output -- Processing locus 10: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAATGCTTAAAGGAACTAAAACTGGTAATTTTCCTACACTACTAAGTTTGATTCACATGTAAAATGAGATTCTTAGCTC TTGGTTTAACTGCTTACATGAAAAGTACTACCATCGTCTGATGCAGAAATAGATGAAAAGGTGAACAAAATCTTGGGAAT GGTTGAGAGTGGAGACGTCAATGAAGATGAGTCCAATAGACAAGTGGTTGCAGACTTAGTGAAGGAATTCTACAGCGAAT ACCAGTCCCTGTACCGCCAGTACGATGATCTAACTGGAGAGATTAGGAAAAAGGTCAACGGGAAAGGAGAAAGCTCCTCT TCATCAAGCTCAGACTCGGATTCTGACCATTCTTCTAAGAGGAAGGTCAAGAGAAATGGAAATGGAAAAGTAGAGAAAGA TGTAGAGTTGGTAACAGGTGCCCTGAAGCAACAAATCGAAGCTGCAAATCTTGAAATTGCTGATCTGAAAGGGAAGTTGA CAACGACTGTTGAAGAAAAAGAAGCAGTAGATTCTGAGCTTGAATTAGCTTTGATGAAGTTAAAAGAATCAGAAGAGATT AGCAGTAAGTTGAAACTTGAAACTGAGAAGTTAGAGGACGAAAAGTCAATAGCACTGAGTGATAACAGGGAACTGCATCA GAAACTGGAAGTTGCTGGCAAAACAGAAACTGATCTGAACCAGAAGTTAGAAGATATAAAAAAAGAGAGAGATGAACTGC AAACTGAGAGGGACAATGGTATCAAAAGATTTCAAGAAGCTGAAAAAGTTGCAGAAGATTGGAAAACAACGAGTGATCAG CTCAAAGATGAAACTTCTAATCTCAAGCAGCAGCTTGAAGCATCAGAGCAGCGAGTTTCAGAGCTGACCAGCGGTATGAA TAGTGCAGAGGAAGAGAACAAATCTCTATCCTTGAAAGTTTCGGAGATTTCAGATGTGATCCAACAGGGACAGACCACCA TACAAGAACTAATTTCCGAATTGGGAGAGATGAAGGAAAAGTACAAGGAAAAAGAGAGTGAGCATTCTAGTTTGGTGGAG TTACATAAGACCCATGAGAGAGAATCATCAAGTCAGGTGAAAGAATTAGAAGCACACATAGAATCATCAGAGAAGTTGGT TGCAGATTTCACCCAAAGCCTGAACAATGCAGAGGAAGAGAAAAAACTGCTATCTCAGAAAATAGCAGAACTCTCTAACG AGATTCAAGAGGCGCAGAACACCATGCAAGAACTCATGTCTGAGTCTGGGCAGTTGAAAGAGAGCCACAGTGTGAAAGAG CGAGAACTTTTCAGTTTGAGGGACATCCATGAGATTCATCAAAGAGACTCATCCACCAGAGCAAGTGAATTAGAAGCTCA ACTGGAGTCCTCAAAACAGCAGGTCTCAGATTTGAGTGCGAGTCTGAAAGCTGCAGAGGAAGAAAACAAAGCTATATCCT CGAAAAACGTGGAAACTATGAACAAACTCGAACAAACGCAGAACACGATACAGGAACTCATGGCTGAATTGGGAAAGTTG AAAGACAGCCACAGAGAGAAAGAGAGTGAGCTTTCTAGTTTGGTGGAAGTACACGAGACTCACCAGAGAGATTCATCAAT TCATGTGAAAGAATTAGAAGAGCAAGTGGAATCATCAAAGAAATTGGTTGCGGAGTTGAACCAAACCCTGAACAATGCAG AGGAAGAGAAAAAAGTGCTATCTCAGAAAATAGCAGAACTTTCTAACGAGATTAAAGAGGCACAAAACACCATACAAGAA CTCGTGTCTGAGTCTGGGCAGTTGAAAGAGAGCCACAGTGTAAAGGATAGAGATCTTTTCAGCTTGAGGGACATCCACGA GACTCATCAAAGAGAATCATCCACTCGCGTGAGTGAATTAGAAGCTCAACTGGAATCCTCAGAACAGCGGATCTCAGATT TGACTGTGGATCTGAAGGATGCAGAGGAAGAAAACAAAGCTATCTCCTCGAAAAATTTGGAAATTATGGACAAGCTTGAA CAGGCTCAGAACACGATAAAAGAACTCATGGATGAATTGGGAGAGTTGAAAGACCGACACAAAGAGAAAGAGAGTGAGCT TTCTAGTTTGGTGAAGTCAGCAGATCAACAGGTTGCAGATATGAAGCAGAGTCTGGACAATGCAGAAGAAGAGAAAAAAA TGTTATCTCAGAGAATCTTAGATATCTCTAATGAGATTCAAGAAGCACAAAAAACCATACAAGAACACATGTCTGAGTCT GAACAGTTGAAAGAGAGCCACGGTGTGAAAGAGAGGGAACTTACTGGTTTGAGGGACATTCACGAGACTCATCAAAGAGA ATCATCCACTCGTTTGAGTGAATTAGAAACTCAACTGAAATTATTAGAACAACGGGTCGTAGATCTGAGTGCGAGTCTGA ATGCTGCAGAGGAAGAAAAGAAGTCCCTGTCCTCAATGATCTTGGAAATTACGGATGAGCTCAAACAGGCCCAAAGCAAG GTACAGGAACTTGTGACTGAATTGGCAGAGTCTAAAGATACACTCACACAAAAAGAGAATGAGCTTTCTAGTTTTGTGGA GGTACACGAGGCCCATAAGAGAGATTCCTCAAGTCAGGTGAAAGAATTAGAAGCACGGGTGGAATCAGCAGAGGAACAGG TTAAAGAATTGAACCAGAACCTGAACAGTTCAGAGGAAGAGAAGAAAATTTTATCTCAGCAAATTTCAGAAATGTCGATC AAGATCAAGCGGGCGGAAAGCACCATACAAGAACTCAGCTCTGAGTCTGAACGATTAAAAGGGAGCCACGCTGAGAAAGA CAATGAACTTTTTAGCTTGAGGGATATCCATGAGACTCATCAGAGAGAATTATCCACTCAGTTAAGAGGTTTAGAAGCGC AACTGGAATCATCTGAACACAGGGTTTTGGAATTGAGCGAAAGTCTGAAGGCTGCAGAAGAAGAAAGCAGAACTATGTCC ACGAAAATCTCAGAAACTTCAGATGAGCTTGAACGGACACAGATCATGGTACAGGAACTCACAGCTGATTCGAGCAAACT GAAAGAGCAGCTCGCTGAGAAAGAAAGCAAACTATTCCTTCTGACAGAGAAGGACAGCAAATCACAGGTGCAAATAAAAG AACTAGAAGCAACAGTAGCGACACTGGAGCTGGAACTAGAGTCAGTTCGTGCCCGTATAATAGATCTTGAGACAGAGATT GCAAGCAAGACCACCGTAGTTGAGCAGTTGGAAGCGCAAAACAGAGAAATGGTTGCTAGAATCTCAGAACTTGAGAAGAC AATGGAGGAGAGAGGAACTGAACTCTCAGCTTTAACTCAAAAACTTGAGGATAACGATAAGCAATCATCGTCTTCAATTG AGACTTTGACAGCTGAGATCGATGGCCTACGAGCAGAATTAGATTCAATGTCTGTTCAAAAAGAAGAGGTGGAGAAACAA ATGGTGTGCAAAAGCGAGGAAGCCTCAGTGAAGATTAAACGTTTGGATGATGAGGTCAATGGTCTGAGACAGCAAGTGGC CTCACTTGATAGCCAGAGAGCAGAACTCGAGATCCAACTTGAAAAGAAGTCCGAGGAGATATCTGAATATCTGAGTCAGA TTACAAATCTAAAAGAGGAGATCATAAACAAGGTTAAAGTTCACGAGAGTATTCTAGAAGAAATAAATGGTTTATCTGAG AAGATTAAGGGTCGTGAACTTGAGTTAGAGACTCTAGGGAAACAGAGAAGTGAGCTTGATGAGGAGCTGAGAACTAAGAA AGAAGAGAATGTTCAAATGCACGATAAGATCAACGTAGCGTCTTCTGAAATAATGGCCTTAACAGAACTGATCAACAATC TGAAGAATGAGCTTGATTCTCTACAGGTGCAGAAGAGCGAAACCGAAGCAGAGCTTGAGAGAGAGAAGCAAGAGAAATCA GAATTGTCGAATCAGATCACCGATGTCCAGAAAGCATTGGTAGAGCAAGAAGCTGCTTACAATACGCTGGAAGAGGAACA CAAGCAGATAAACGAACTATTTAAAGAAACTGAAGCAACACTAAATAAGGTAACTGTGGATTACAAAGAAGCTCAAAGAT TGTTGGAGGAGAGGGGTAAGGAAGTGACATCCAGAGATTCTACAATTGGGGTTCATGAAGAGACGATGGAGAGTTTACGT AACGAGCTGGAAATGAAAGGAGACGAGATCGAAACTCTCATGGAGAAGATCAGTAACATCGAGGTTAAGCTACGCTTGTC GAACCAGAAACTGAGAGTAACCGAACAGGTACTAACAGAGAAAGAAGAAGCTTTCAGGAAAGAAGAGGCTAAGCACTTAG AGGAGCAAGCATTGCTTGAGAAGAATCTCACCATGACACATGAGACTTATCGAGGTATGATCAAAGAGATAGCAGATAAA GTGAACATAACAGTAGATGGGTTTCAATCCATGTCAGAAAAACTCACGGAGAAACAGGGGAGATACGAGAAAACTGTAAT GGAGGCATCAAAAATACTGTGGACTGCGACGAATTGGGTGATAGAGAGAAATCACGAGAAGGAGAAGATGAATAAAGAGA TAGAGAAGAAAGATGAAGAAATAAAAAAGCTTGGAGGAAAAGTAAGAGAAGATGAAAAAGAGAAGGAGATGATGAAAGAG ACTTTGATGGGACTTGGAGAAGAGAAAAGAGAAGCGATAAGGCAATTATGTGTTTGGATCGATCACCATAGAAGTCGTTG TGAATATCTTGAGGAGGTTTTGTCTAAGACCGTTGTGGCTCGAGGCCAAAGAAGAGTGTCGCAGCGAACTTAAGGACTCA GTGCCATGGTACGTTGTTTATCTTTCCCAAGCCTAGTTTCCACCTCACATGAATGCAAACAATGAAACAACATATATGTG TTTATTAGGTTT ...i.....................................................N......i............... .................N......................N...........N.........................i. ....................N.....N..................................................... ........................N....................................................... .......................................................N.....i.................N ................................................................................ .....................................................N.......................... ................................................................................ .......................................................................i........ ...............N................................................................ .......N...................................................................i.... .....................................................N.......................... .............................N.................................................. .............N.................................................................. ..........................i..................................................... .......................i...........i............................................ ............................N................................................... ................................................................................ .................N.........................................i.................... ................................................................................ ..N.......................................................................i..... ................................................................................ ................................................................................ ................................................................................ ..................i..............................................i.............. ...........................i...N................................................ ........................................N..................N...................i ..............................N.....................................i........... ................................................................................ ................................................................................ N..................................N..................N......................... ..........................................................i..................... ................................................................................ .......................................................................i........ ................................................................................ ..i..........................N.................................................. ..........................................................................i..... ......................N......................i.................................. ................................................................................ ................................................................................ ................................................i............................... .....................N.........................i................................ i.............................................N..N........N..................... ................................................................................ ..................................................................N............. ..........................................................N..................... ........N.......i...................................i........................... ......N......................................................................... ......................N......................................................... ................................................................................ ......................................................N..........i.............. ............N..........................i........................................ ................................................................................ ................................N......N................N....................... ................N.............N...............................................i. ...................................................................i............ ............N......................................N................N..N........ ......N........................................N................................ ................................................................................ .....N..........................................N...N........N.............N.... ............ Pos Score Pred ------------------------ 4 0.577 Yes 58 0.231 - 65 0.550 Yes 98 0.187 - 121 0.168 - 133 0.135 - 159 0.655 Yes 181 0.303 - 187 0.327 - 265 0.315 - 376 0.217 - 382 0.549 Yes 400 0.295 - 534 0.456 - 712 0.507 Yes 736 0.222 - 808 0.141 - 876 0.525 Yes 934 0.248 - 990 0.295 - 1054 0.165 - 1147 0.550 Yes 1224 0.512 Yes 1236 0.746 Yes 1309 0.353 - 1458 0.492 - 1500 0.670 Yes 1603 0.066 - 1675 0.605 Yes 1939 0.538 Yes 1986 0.518 Yes 2028 0.710 Yes 2032 0.205 - 2121 0.410 - 2140 0.302 - 2160 0.696 Yes 2191 0.216 - 2229 0.757 Yes 2401 0.096 - 2436 0.333 - 2455 0.342 - 2539 0.593 Yes 2712 0.582 Yes 2803 0.615 Yes 2830 0.469 - 2955 0.559 Yes 2983 0.331 - 3006 0.510 Yes 3249 0.750 Yes 3282 0.672 Yes 3382 0.295 - 3408 0.807 Yes 3441 0.660 Yes 3487 0.104 - 3490 0.299 - 3499 0.165 - 3667 0.373 - 3739 0.136 - 3769 0.163 - 3777 0.677 Yes 3813 0.862 Yes 3847 0.377 - 3943 0.168 - 4135 0.066 - 4146 0.721 Yes 4173 0.287 - 4200 0.733 Yes 4353 0.460 - 4360 0.209 - 4377 0.371 - 4417 0.179 - 4431 0.292 - 4479 0.688 Yes 4548 0.545 Yes 4573 0.435 - 4612 0.263 - 4629 0.293 - 4632 0.211 - 4647 0.217 - 4688 0.458 - 4806 0.111 - 4849 0.059 - 4853 0.029 - 4862 0.048 - 4876 0.069 -

Explain the output -- Processing locus 11: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCTAGATTCAATAAGATAAGATTTAAAATAAATTTAAGAATATCATGATTAAAAAATTGAATTAAAAGATTCATGAGCT TGATTCGGTAATGCCATATAAAAAGATGTCATCACAATATACATGGTGATTTGTTGGAAGATTTCGGAACCAATGGGTCG GAAGAAGCCGAGTAGTTCTCGAGGTGGAGATGATGAGCAACCACCTGCTGCGTCATCTCTCGTTGGTGCTGAATCCAAGA AGAAAGCTGTCCAAAGTGATGAAGAAGATAAGTACTCCATAAATACTGAGGAAGAGAAAGTTGTTATCACTGGGAAGAAG AAGAGTAATAAGAAGGTTACGCAGAAGCATGATGACGACGATGATTTTACTGAGGCTGTTCCCGAGAATGGTTTTGTAGG GAAGAAGAAGAAGTCCAAAGGTAAGAACAGAGGTGGCTCTGTTAGCTTTGCCCTGCTTTCTGGTAAGGAGGAAACGGATG ATAATGAATCTAATGGGGACAAAGATGATGAGCCAGTTATAAGTTTTACAGGTAAGAAGAATGCGTCTAACAAGGGTAAA AAAGGCTTTGCGGTTTCAGCTTTTGATGCCCTTGGTGGTGATAAGGATGACGAGGAAGTGGATGGAGATGAGGAACAAGT TTCACCTATCACTTTCTCTGAAGGGACAATGGGAGACGAAGAGAGCCTCGAAATCACGTTTTCCGGTAAAAAGAAGGGCA GCATTGTTTTGGCCTCACTAGGTGATGACTCAGTTGCCGACGAAACTAGTCAAGCTAAAACTCCTGATACTAAGAGTGTG GAGGTTATAGAAACTGGAAAGATCAAGAAAAAGAAGAAGAATAAGAATAAGGTTGCAAGGACATTAGAGGAAGAGGATGA TTTGGACAAACTTCTTGCAGAACTTGGAGAAACCCCTGCTGCTGAAAGACCTGCTTCATCGACACCCGAAGTAGAGAAAG TTCAAGCTCAGCCTGGGCCAGTTGCACCTGTAGAGAATGCTGGTGAGAAGGAGGGTGAAAAAGAGACTGTTGAGACTGCT GCAGCGAAGAAAAAGAAAAAGAAGAAGGAGAAAGACAAGGAAAAAAAAGCAGCTGCTGCAGCAACCTCTTCTGTAGAGGC TAAGGAGGAAAAACAAGAAGAATCAGTTACTGAGCCACTACAGCCGAAGAAGAAGGATGCAAAGGGTAAAGCAGCAGAGA AGAAAATTCCTAAACATGTTAGAGAGATGCAAGAGGCTCTTGCACGGCGGCAAGAAGCAGAAGAGAGAAAAAAGAAGGAA GAAGAAGAAAAATTAAGGAAGGAGGAAGAGGAGCGCCGCAGGCAGGAAGAGCTTGAGGCTCAGGCTGAGGAGGCTAAGCG TAAGAGAAAGGAAAAGGAAAAGGAGAAACTGTTGAGGAAGAAGCTAGAGGGCAAGCTTCTAACAGCAAAGCAAAAGACTG AAGCTCAAAAGAGGGAGGCGTTTAAGAACCAGCTGCTGGCTGCTGGTAGAGGTCTTCCTGTTGCAGATGATGTTGGTGAT GCTACTTCCTCAAAGCGTCCCATTTATGCCAACAAGAAAAAACCATCTCGCCAGAAAGGCAATGACACTTCTGTCCAGGT GGAAGATGAGGTAGAGCCACAAGAAAACCATGCAGCTACTTTAGGTGAAGTGGGTTCAGAAGACACTGAAAAGGTTGATT TACTAGAGTCAGCAAACACAGGCGAGAAATCAGGTCCTGCTGATGTAGCTCAGGAGAATGGGGTTGAGGAAGATGATGAA TGGGATGCAAAAAGCTGGGATAATGTTGATCTTAAAATTGACGATAAAGAGGAAGAAGCTCAGCCTGTGGTTAAGAAAGA ATTAAAGGCACATGATTCAGACCATGAAACTGAAAAGCCAACAGCTAAACCAGCAGGTATGAGCAAGCTAACAACAGGTG CGGTAAAAGCTATTTCTGAAGTAGAAGATGCTGCTACACAGACAAAACGTGCTAAGAAGGGTAAATGCTTAGCTCCAAGT GAATTTATAAAAGAAGGTGGAGAAAATCTCCGTTCTATTATTTGCTGTATCATGGGTCATGTCGATAGTGGTAAAACAAA GCTGTTGGATTGCATCAGAGGAACAAATGTACAGGAAGGTGAGGCTGGAGGTATAACTCAGCAGATTGGTGCAACATATT TCCCTGCCAAAAACATCCGAGAGAGGACCAGGGAACTGAAAGCGGATGCAAAACTTAAGGTGCCAGGTCTATTGGTTATC GATACACCTGGCCATGAGTCCTTTACTAATCTGCGGTCAAGAGGTTCAAGTTTGTGCGACCTTGCAATTTTGGTGGTTGA CATAAAGCATGGGTTAGAGCCACAAACCATAGAATCTCTAAATCTTTTGAGGATGAGGAATACAGAGTTCATTATTGCAT TGAATAAGGTGGATAGGCTATATGGATGGAAAACATGCAAGAATGCTCCTATCGTGAAGGCAATGAAGCAGCAAAATAAA GATGTTACGAATGAATTCAACATAAGGCTGACTGATATTATAACCCAATTCAAGGAGCAAGGACTCAACACTGAGATTTA TTACAAGAATAAAGAAATGGGAGAAACTTTCAGTATTGTTCCTACTAGTGCTAAAAGTGGAGAAGGGGTCCCAGATCTCT TGCTGTGGTTGGTTCAATGGGCTCAGAAAACAATGGTTGAGAAACTTACATATGTTGACGAAGTGCAGTGTACTGTACTT GAGGTCAAAGTTATTGAAGGCCATGGGACAACGATTGATGTTGTGTTGGTAAACGGTGAACTCCATGAAGGTGATCAAAT CGTCGTTTGTGGGCTACAGGGACCTATTGTCACAACGATTAGAGCATTACTGACTCCTCATCCAATGAAGGAGTTAAGGG TGAAGGGTACTTATCTGCATCACAAAGAAATAAAGGCTGCACAAGGCATCAAAATTACTGCCCAGGGCCTTGAACACGCA ATTGCTGGTACTTCCCTGCATGTGGTTGGACCTGATGATGACATCGAGGCAATGAAGGAGTCGGCTATGGAAGATATGGA ATCGGTTTTGAGCAGGATTGACAAAAGTGGTGAAGGAGTTTATGTACAAACGTCTACATTAGGGTCATTAGAAGCATTGC TTGAATTCTTGAAGACCCCAGCTGTAAATATACCTGTCAGTGAAGGATATAATGAAGGCTGGAGTGATGCTCGAGAAGAA AAGGAATATGCTACAATTTTGGCTTTTGATGTGAAAGTGACTACAGAGGCTCGTGAACTTGCAGACGAAATGGGAGTAAA AATCTTCTGCGCTGATATCATCTATCAGTTATTCAATCAGTTTCAGGTTTACATTGAGAATATCAAAGAGGAGAAAAAGA AGGAATCAGCCGGTGAGGCAGTCTTCCCATGTGTCCTTCAGATTTTACCTAACTGTGTGTTCAATAAGAGAGACCCAATA ATCCTTGGAGTTAAGGTCCACGACGGTATACTTAAGATCGGAACCCCCATTTGCGTCCCGGGCAGAGAGTTCACAGATAT TGGCCGCATTGCCTCTATTGAGAACAACCACAAGCCTGTTGACTATGCAGAGAAGGGCGACGAGGTGGCGATTAAGATTG TTGCTTCAAATCGCGAAGAACAGAAGATGTTTGGAAGGCATTTTGACATGGAAGATGAGCTTGTGAGTCACATTTCGAGG AGATCTATCGACATACTCAAAGCTGACTACATGGTAAGTCAAGGATAAGATAAGTTGTTTCCCATCTACGTTTTCTTTGA CTCATTATTCTGACCCAAAACAAATGCTCGAAATTAAAACCAATAACAATTGCAGAAAGAAATGTCG .............................................N...........................N...... ..........N..............i................N.............................i....... .............................N..N............................................... ..................N............................................................. ............................N..N........N..........................N............ .............................................................................N.. ...N........N...........N..N................................N................... .........................N....................N..............N.....N............ ............................i................................................... ........................N....................................................... ............................................................................N... ................................................................................ ....................................N........................................... ................................................................................ ........................................................N....................... ...............N..........i..................................................... ................................................................................ ................................................................................ ..................................................................N..N........N. .........................N...................................N.................. .....N.......................N.................................................. ..........................................N..............N..............N..N...N ....N.................N......................................................... ...........N...........N..................................i..................... ...........................N....................................N............... ...................................................i......N..................... ..........................N..................................................... .............................................N.................................. .............N.................................................................. ........N...........................................N........................... .....................N...N........N.......N...................N................. ................i............................................................... ................N...............i..................N............................ ......................N..............N..........................N............... ................................................................i............... ................................................................................ ...................N..............N..N.............N..............i........N.... .........................................N...................................... ...................................................N..............N............. .......N....................N........................................i.......... ................................................................................ ............................N................................................... ................................................................................ ............................................N................................... ..........................N....................i......N......................... ..............................N................................................. .......................N.....................................N..... Pos Score Pred ------------------------ 46 0.104 - 74 0.473 - 91 0.281 - 106 0.501 Yes 123 0.353 - 153 0.815 Yes 190 0.184 - 193 0.208 - 259 0.173 - 349 0.153 - 352 0.238 - 361 0.069 - 388 0.137 - 478 0.154 - 484 0.101 - 493 0.118 - 505 0.100 - 508 0.190 - 541 0.144 - 586 0.206 - 607 0.281 - 622 0.219 - 628 0.136 - 669 0.839 Yes 745 0.122 - 877 0.267 - 997 0.154 - 1177 0.457 - 1216 0.492 - 1227 0.610 Yes 1507 0.266 - 1510 0.223 - 1519 0.209 - 1546 0.129 - 1582 0.260 - 1606 0.307 - 1630 0.145 - 1723 0.307 - 1738 0.267 - 1753 0.238 - 1756 0.084 - 1760 0.200 - 1765 0.096 - 1783 0.229 - 1852 0.200 - 1864 0.190 - 1899 0.661 Yes 1948 0.128 - 1985 0.264 - 2052 0.797 Yes 2059 0.338 - 2107 0.450 - 2206 0.188 - 2254 0.210 - 2329 0.313 - 2373 0.325 - 2422 0.145 - 2426 0.492 - 2435 0.461 - 2443 0.202 - 2463 0.443 - 2482 0.265 - 2491 0.305 - 2577 0.802 Yes 2657 0.405 - 2673 0.720 Yes 2692 0.339 - 2743 0.154 - 2758 0.255 - 2785 0.123 - 2865 0.686 Yes 2980 0.187 - 2995 0.113 - 2998 0.212 - 3012 0.404 - 3027 0.567 Yes 3036 0.415 - 3082 0.179 - 3172 0.253 - 3187 0.202 - 3208 0.071 - 3229 0.060 - 3270 0.789 Yes 3389 0.315 - 3565 0.099 - 3627 0.356 - 3648 0.606 Yes 3655 0.142 - 3711 0.159 - 3784 0.067 - 3822 0.131 -

Explain the output -- Processing locus 12: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATATCTGTAATCGTACTTTACCCTGAAAATATGTACCTACCATATGTGTCATGTGTATTTTTATTATTTTATAAATGGT TGTGATATTATTGAGACATGAATGAATGATGCTTCAATTGTATGTAGTGTTGGCACAGAAGTAATGGGGACAGAGCTAAT GAGAATCTGTGTGAAAGAAGATAGTGATGATTTGCCATCTGTTCCTCCTGGTTTTGAATCATATGCAACTTTTACCTTAA AAAGAGTAGTCCCTGCTACTACTTCTGATAAAGCCAAGACTCCTGCAATTGAAAGTGTTTCTGCAACCGAACAAGCTAAG ATGGAAGTTGAATCTGATGAAGCCAAAGCTGCTCGGGCTCTACGCCGTAGACCTTGGATTAATCATAGCGGATGTGATGA TGATGGTGATTGTGCTGCAAACAATGACAATGCTGCATCTCAAAATCCTGATCAAAATTGTGATGTGAAGCCAGCTCTTC CAAAGGGTGTGGTCAGGGGATGTGAAGAATGTAAAGATTGTCAGAAGGAGTTTGAAGACACTTTAAATTATATCGCTAAA ATAAGACCGGAGGCTGAAAAGTATGGAATCTGTCGCATTGTTCCTCCTCCTTCCTGGAAACCGCCGTGCCCACTGAAAGA AAAGCAAGTATGGGAGGGTTCTAAATTTACAACACGTGTCCAAAGAGTTGATAAACTTCAGAATCGGAGTTCAATGAAGA AGATTTCAAAGCTTCCTAATCAAATGAGAAAGAAGAAAAGAAAGTGCATGAAAATGGGAATGGATTCTGTCACTAATGGT ATGGGCGATCCCTGTTCTGCAAGCACTGGAATGAATGAACTTGAGACGTTTGGGTTTGAACCTGGTCCAGGGTTCACACT AAAAGACTTCCAAAAATATGCTGATGAGTTCAAGGCTCAGTACTTTAAAAAGAGTGAAACTTCTACAGACGATAAATGTA AAGTTGATAATTCAATAGACTGCTGGGAGCCAGCACTTGAGGATGTTGAAGGTGAATATTGGCGGATAGTAGATAAAGCA ACTGAAGAGATAGAGGTGCTATATGGTGCTGACCTTGAAACTGGGGTATTTGGTAGCGGATTCCCCAAGATATCATCAAG TCACAATGCTTCTTCTTCAGAAGATAAATATGCAAAATCAGGCTGGAACTTAAATAACTTTCCAAGGCTTCCGGGATCCC TCCTTAAGTACGAGGGCAGTGATATTTCCGGTGTCCTTGTGCCGTGGCTGTATATTGGGATGTGCTTTTCTTCTTTCTGC TGGCATGTTGAAGATCACCACTTGTATTCGTTGAATTATATGCACTGGGGTGCACCGAAATTGTGGTATGGTGTTGGGGG GAAGGATGCTGTTAAACTCGAGGAGGCAATGAGGAAGCATTTGCCTGACCTTTTTGAAGAACAGCCTGACTTACTTCATA AGCTAGTTACACAACTCTCCCCGTCAAAACTGAAAACCGCAGGAGTACCTGTGCACCGTTGCGTCCAGCATGCTGGAGAG TTTGTCTTGACTTTTCCTCGGGCATATCATGCTGGATTTAATTCTGGTTTCAACTGTGCTGAAGCTGTGAATGTAGCACC CGTTGACTGGTTACCTCATGGGCAGATTGCCATAGAGTTATACTGTCAACAGGGTAGAAAAACGTCCATCTCGCATGATA AATTGTTGCTTGGGGCAGCAAGGGAAGTAGTGAAAGCCGACTGGGAGCTGAACTTACTAAGGAAAAATACTGTAGATAAC TTAAGGTGGAAAGCATTCAGTGCGAAGGATGGAATTTTGGCAAAAACATTAAAGGCACGCATTGACATGGAACGTACGAG AAGAGAATTTCTGTGCAACTCTTCACTTGCATTGAAAATGCACAGTAATTTCGATGCTACCAACGAGAGAGAGTGCTGTA TATGCTTCTTTGATTTGCACCTGTCTGCTGCTGGCTGTCGTTGTTCCCCGGAGAAGTATTCATGTTTGACTCATGTGAAG GAGTTGTGTTCATGTCCATGGGTTACTAAATATTTTCTATTTCGCTATGATATCGATGAACTGAATGTTCTTGTTGAGGC AGTGGAAGGAAAACTGAGTTCTGTATATAGATGGGCGCGTCAAGATTTAGGATTGGCATTAAGTACAGACGTCTCAGGAA GCAAGATGGAGATAGATGAAGAAGGAAAAGTCCACAAGGACCCGACTCCACAAACAACTGCACTTTCAGGGAAGGATTTG CAGTTGAAAGTAACATCGAAAGAAGTAAGTAAAGAGTTAGAAAAGACTAGTAAATTATCTCATGTTAATTTACTTCTGAA AGAAAAGGAGGAGCAAATAACGTCAAGTCACTGTATGAAGCCTGTCAAAGAAGAAACTGTTTGTGATTCTTCTGATCCAA ACGTTTCAGCTTGCCAACCATCTGAAGGAGGCATAATCTGTATGACAGCTGTTAAGTCCGCAAGTGGTAAAAAGAATTCA CAGAGTCTACCCAATGATGTGATACTCCTCAGCGATGATGAGTATGACATACCTAGGAAACGAGGTTCTGTGAGAAGAGA TGCAATTTCTTCTGGCAAGAAATTAGAAATACGAGAGAGACCGACCCACGTTTTGGCGTTAGAAGCTTCTGCCAAAATTG CTGCTCCAATTTGCCAAAGGGAAGGAGATTCGCTGCGTGATACACGAAACACTATATCATTGCCTACTAATGACCAAAAA ACAATGAGAAGGGATGTACCGAGTTCTACTTCGCACGCGGAAGTTAATGCGGAGGCTACTGGGCTTACTCAAGATATATG TAACAGAATGGCAACTAACAGCCACGGTGGTGGAAAACCTACTAGTTGTAAATCCAAAAACTCTGGAGGTTTGGCTATAG TGGATGTTGTGGATGGGACAAGAAGTAGTTCAGGTACACCGTCTTGTTCGCAAAATAATAGTCCGGATAGGTTCATCCGC CAAAAGGGTCCCCGCATTGCAAAGGTCGTGAGGAGAATCAATTGCAATGTAGAGCCTTTAAGTTATGGATGTGTGCTGTC TGGAAAATCATGGTGCAGCCGGCGAGCAATTTTTCCTAAAGGATTTCGTAGCCGGGTTAAGTACATAAACATTTTGGATC CAACAAATATGTGCTTCTACATTTCAGAAATTCTGGATGCTGGACGCAACAGTCCATTGTTCATGGTTTACTTGGAGAGT AATCCCAGTGAGGTGTTCGTTCATATGTCGCCTACAAGATGCTGGGAGATGGTAAGAGAGAGAGTAAATCAAGAGATAAC TAAGCAGCACAAAGCTGGAAAATCAGATCTTCCTCCTTTGCAACCCTCTGGGAGTCCCGACGGTTTTGAAATGTTTGGAT ATTCGTCACCCGCAATCGTACAGGCTATCGAAGCATTAGACGTGAATCGAGTTTGCACAGACTATTGGGATTCCAGGCCT TATTCCCGGCCACAAGTTCAGTTCCCTGCAAATCCTCTTCTCAGAGAAGCCAACACAAGTGGCAGATCGAATGTGGGAAA TCTCCAGCTAAACCCTGGACACCATATATCGCCTACTGGAATAAATTCTATTCTTAAAGTTCTGTTCAAGAAAGCTAGCA TGGAGGAACTAAGCTCACTTCAAGAGGTTTTAAGTGAGACTAACTCAGATATGGTGACCGAACTTGTGAAGGAAGAGATC CAGAACCGTCGCTGATTTGATTCAAAGGGGATTGATTTTAGAGTGTACATACGGAGTTGAATCCACTCACTGTTTTGCAG GATGAGGCTCCCTTCCCTACTCATTCTTTTTGGA ...............................N............i......N.......................N.... .................N...N...N..N............N.....................i..............N. ..........................N...................................N................. ................................................................................ i...............N......................................................N....N..N ..N....................N.....N................................N................. ...................N........N................................................... ......................i......................................................... .........i...............................................................N...... .......................i.......................i.....i.....i...............N.... N.............................N...N............................................. .................N.....N...................................................N.... ..........................................N..................................... ......................N......................................................... .....N.......................N.................................................. ...........................................................N.................... ....N..................................i...........................N............ .....N......................N................................................... .....................................................................N.......... ............................N.........................................N......... .................N........................................................N..... ................................................................................ ............................N.....................................N............. .....................................N...............N.......................... ...........N.....N............................N........N........N............... ..............................i................................................. .....i.........N................................................................ .............................................................N.................. ..................................N............................................. .........................................i...................................... .............N..N.................N..i.....N...................................N ................................................................................ .....................................................................N.......... ...i.........i................................i..............................N.. .......N........................................................................ ...N........N................................................................... ..............................................i.................N...N........... .........N...................................................................... ........N...........................N.........................i................. ........................i.............i.........i............................... ......................................................................i......... ................................................................................ ......................................................................N......... ...............................................................................i ..................................................i............................. ................................................................................ Pos Score Pred ------------------------ 32 0.132 - 45 0.570 Yes 52 0.384 - 76 0.440 - 98 0.382 - 102 0.475 - 106 0.176 - 109 0.110 - 122 0.184 - 144 0.770 Yes 159 0.451 - 187 0.240 - 223 0.086 - 321 0.915 Yes 337 0.337 - 392 0.109 - 397 0.218 - 400 0.388 - 403 0.315 - 424 0.302 - 430 0.326 - 463 0.146 - 500 0.207 - 509 0.321 - 583 0.637 Yes 650 0.617 Yes 714 0.416 - 744 0.583 Yes 768 0.641 Yes 774 0.710 Yes 780 0.627 Yes 796 0.266 - 801 0.420 - 831 0.328 - 835 0.179 - 898 0.341 - 904 0.380 - 956 0.319 - 1003 0.239 - 1063 0.326 - 1126 0.245 - 1150 0.268 - 1260 0.180 - 1285 0.312 - 1320 0.564 Yes 1348 0.314 - 1366 0.248 - 1389 0.495 - 1510 0.306 - 1549 0.176 - 1591 0.320 - 1618 0.410 - 1675 0.125 - 1789 0.265 - 1827 0.182 - 1878 0.477 - 1894 0.225 - 1922 0.291 - 1982 0.204 - 1993 0.313 - 2012 0.254 - 2018 0.450 - 2047 0.389 - 2056 0.309 - 2065 0.176 - 2111 0.530 Yes 2166 0.756 Yes 2176 0.255 - 2302 0.140 - 2355 0.380 - 2442 0.597 Yes 2494 0.316 - 2497 0.311 - 2515 0.241 - 2518 0.514 Yes 2524 0.225 - 2560 0.178 - 2710 0.309 - 2724 0.843 Yes 2734 0.531 Yes 2767 0.625 Yes 2798 0.143 - 2808 0.431 - 2884 0.246 - 2893 0.136 - 3007 0.645 Yes 3025 0.489 - 3029 0.332 - 3050 0.280 - 3129 0.394 - 3157 0.253 - 3183 0.575 Yes 3225 0.640 Yes 3239 0.530 Yes 3249 0.721 Yes 3351 0.573 Yes 3511 0.218 - 3600 0.531 Yes 3651 0.659 Yes 3762 0.045 -

Explain the output -- Processing locus 13: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATAATGTGATTTTCTCTGTTTGTATTTGTTTTTAAAAAAAAAAACTTGCAAGGACAATATAATGATTAGAATAATGT TTTTTCCCCTCCAACAAATAGTATCATTTTTTTAGTAAATGCATGGATTATAGTGCGGACTTCAAATTATCTACAGAACC AGATTTTTGTATTTCTTTTACTTTAACTTTACTCTTTTTCTGCTCATTCTCTCTCCCAGAAAAAAACCACACTCATGGCG ACACTTCTCTCACCTTCTTCAAGCTAAGTTTTCATTTTGTTCACTGCCACAGATCTATGGCCATGGATATATCTCTGTTC TTCATTTTCCTGGTCATTTACGCGCCGTTAGTCTCATACGCCGATGAATCTCAAGCCGAGATTGATGCTCTCACGGCGTT TAAACTCAACCTCCATGACCCTCTCGGTGCTTTAACTTCATGGGATCCTTCAACACCAGCAGCTCCATGTGACTGGCGTG GCGTTGGCTGTACTAACCACCGTGTCACTGAGATCCGTCTTCCTCGTCTCCAACTCTCCGGACGAATCTCCGATCGTATC TCCGGTCTACGCATGCTACGGAAGCTCAGTCTCCGGTCTAACTCTTTCAACGGTACTATCCCTACTTCACTCGCTTACTG CACGCGCCTACTCTCCGTCTTTCTTCAGTACAACTCACTCTCCGGTAAACTACCTCCGGCGATGAGGAATCTCACCTCGC TTGAGGTCTTTAACGTCGCTGGAAACCGTCTCTCCGGTGAGATCCCCGTTGGTTTACCATCGAGTCTCCAGTTCCTTGAC ATTTCGTCGAACACCTTCTCTGGTCAGATACCGAGTGGACTCGCTAACTTGACTCAGCTTCAGCTTCTTAATCTCTCGTA TAATCAGTTAACCGGCGAGATTCCGGCGAGTCTAGGTAACCTCCAAAGTCTTCAGTACTTATGGCTTGATTTCAATCTCT TGCAAGGAACATTACCGTCTGCTATCTCAAACTGCTCCTCCTTAGTTCACTTGAGCGCGTCGGAGAACGAAATCGGCGGC GTGATTCCCGCCGCGTACGGTGCTCTTCCGAAACTGGAGGTTCTCTCGCTCTCCAATAACAATTTTTCCGGTACTGTGCC GTTTTCTCTCTTCTGCAACACATCGCTTACGATTGTTCAGTTAGGGTTCAATGCTTTCTCCGATATCGTACGGCCTGAGA CGACGGCGAACTGTCGTACCGGTTTACAGGTCTTGGATCTCCAGGAAAATCGAATCTCCGGTCGTTTTCCGTTGTGGTTG ACTAATATTTTGTCACTAAAGAATCTTGATGTTTCTGGAAACTTGTTTTCCGGTGAGATTCCGCCGGATATTGGTAACTT GAAGAGATTAGAGGAACTGAAATTGGCTAATAACTCTCTTACTGGTGAGATTCCTGTTGAGATTAAGCAATGTGGTTCGT TAGATGTTTTAGATTTCGAAGGAAATAGTTTAAAAGGTCAGATTCCAGAATTTCTTGGCTACATGAAGGCTTTAAAGGTT TTATCTTTAGGCAGAAACAGCTTCTCAGGTTATGTTCCTTCGTCTATGGTGAATCTGCAGCAGCTTGAGAGGCTAAACTT AGGTGAAAACAACTTAAATGGGAGTTTTCCGGTGGAGTTGATGGCGTTGACAAGTCTGTCTGAGCTGGATCTGAGTGGAA ATCGGTTTTCCGGGGCAGTTCCGGTTAGTATCAGCAACTTAAGTAACCTTAGTTTTTTGAATTTGAGTGGGAATGGATTT TCTGGTGAGATACCAGCTAGTGTAGGCAATCTGTTTAAGTTAACAGCTCTTGATTTGAGCAAGCAGAATATGTCTGGTGA GGTTCCGGTTGAGCTCTCTGGTTTGCCCAACGTGCAAGTGATTGCATTGCAGGGAAACAACTTCTCTGGGGTTGTACCTG AAGGGTTTAGTAGCTTGGTGAGTTTAAGGTATGTGAACCTTAGCTCGAACTCATTCTCCGGGGAAATACCGCAGACTTTT GGGTTTCTTCGGTTGTTGGTTTCTTTGTCACTGTCAGATAATCACATCTCTGGGTCTATTCCACCTGAAATTGGAAACTG CTCAGCTCTTGAAGTTCTTGAGTTAAGATCTAACCGGCTAATGGGTCATATTCCAGCTGATCTCTCTCGTCTTCCGCGTT TGAAGGTGCTTGACTTGGGTCAGAACAACTTGTCAGGCGAAATCCCACCAGAAATCTCTCAGAGCTCGTCTCTCAACTCA TTGTCACTTGACCACAATCATCTTTCAGGAGTCATACCTGGATCTTTTTCTGGTTTATCAAACTTGACAAAGATGGATCT TTCTGTTAATAACTTAACCGGGGAGATTCCGGCTAGCCTCGCCCTTATTTCTAGTAACCTGGTATACTTCAACGTCTCAA GCAACAACCTTAAAGGAGAGATTCCAGCTTCTTTAGGCTCAAGAATCAACAACACATCAGAATTTTCAGGCAACACAGAG CTCTGCGGGAAGCCATTGAACAGAAGATGTGAAAGCAGCACAGCGGAGGGGAAGAAGAAGAAAAGGAAAATGATTCTGAT GATAGTTATGGCTGCAATTGGTGCATTCCTCTTGTCACTCTTCTGCTGCTTCTACGTTTACACACTCTTGAAATGGAGGA AGAAGCTGAAACAACAGTCTACAACGGGAGAGAAGAAACGCAGTCCTGGTAGAACAAGCGCAGGAAGTAGAGTTCGTAGC AGCACAAGCAGATCAAGCACAGAAAACGGAGAACCAAAGCTAGTGATGTTCAATAACAAGATCACTCTAGCTGAAACAAT TGAAGCAACACGACAGTTCGATGAAGAAAACGTTCTTAGCAGAACCCGATATGGATTGCTATTTAAAGCCAATTACAATG ACGGAATGGTTCTCTCTATCCGCCGCTTACCCAATGGATCACTCTTAAATGAGAACTTGTTCAAAAAGGAAGCCGAAGTT CTTGGTAAAGTCAAGCACCGAAACATCACAGTCCTTAGAGGTTACTACGCAGGTCCACCAGATTTAAGGCTTTTGGTATA TGACTACATGCCCAATGGGAACTTATCTACCCTGCTCCAAGAAGCTTCCCACCAAGATGGCCATGTCCTAAACTGGCCAA TGCGCCACCTCATAGCTCTCGGGATCGCGCGCGGACTCGGATTCCTCCATCAATCCAACATGGTTCATGGTGATATCAAG CCACAGAATGTACTCTTCGACGCTGACTTTGAAGCCCATATATCTGATTTCGGGCTTGACCGTCTCACAATTCGATCCCC ATCAAGATCAGCCGTCACAGCAAACACCATTGGGACACTAGGATATGTCTCTCCAGAAGCCACATTGTCCGGGGAAATCA CAAGAGAATCTGACATCTACAGCTTCGGCATTGTCCTACTCGAAATCCTAACTGGAAAAAGACCAGTAATGTTCACACAA GACGAGGATATAGTGAAATGGGTAAAGAAACAGCTTCAGAGAGGTCAAGTTACAGAGCTACTAGAACCAGGACTACTCGA ACTTGACCCGGAATCATCAGAATGGGAAGAGTTCTTATTAGGAATCAAAGTCGGACTTCTTTGTACTGCAACAGATCCTC TAGACCGTCCAACAATGTCCGATGTTGTTTTCATGCTTGAAGGCTGTCGTGTTGGTCCTGACGTTCCTTCCTCCGCCGAC CCAACCTCTCAGCCTTCACCGGCCTAACCGTTTTTCTTATTTTTAGTCTTCCTGATTTAAACTGTTAGTCAAAAACAAAA AACAAAAAACAAGAAGCAATTATTAAAAATTTCAGCAGTATTTATT ......N.........................................................N...........N... ......................................N...N..................................... ..........................................................................N..... ........................................................i.....i................. ...........................................N....................N............... ..............N........................N..........................i............. ................................................................................ ............N................................................................... .............................................................i.................. ................................................................................ ................................................................................ ............................................................i................... ................................................................................ ................................................................................ ..................................................N............................. ................................................................................ ............................N................................................... .....................................................................N.......... ...N..........................................................N................. ...............................N.............i.................................. .................N......................N....................................... ........................................................................N....... .....................................................................i.......... ................................................................................ ..............................N................................................. ................................................................................ ........................................i....................................... ................................................................................ ........................................................................i....... ................................................................................ ................................................................................ ..........................i..........................................i........N. .......N................................................................i....... ................................................................................ .............................................N.................................. ....................N.............................i..........................i.. .....i...........................N..............i............................... ...............................................................................N .......i......N.........................................i.....i................i ...........................................................i......N............. .......N........................................................................ ............................................i................................... ....................................................................i........... .................i.............................................................. .....................N.......................................................... ..............i......N..........N............................................... ................................................................................ .............................................. Pos Score Pred ------------------------ 7 0.490 - 65 0.183 - 77 0.106 - 119 0.193 - 123 0.192 - 235 0.373 - 297 0.901 Yes 303 0.782 Yes 364 0.215 - 385 0.448 - 415 0.391 - 440 0.210 - 467 0.520 Yes 573 0.427 - 702 0.671 Yes 941 0.760 Yes 1171 0.266 - 1309 0.308 - 1430 0.355 - 1444 0.217 - 1503 0.189 - 1552 0.306 - 1566 0.579 Yes 1618 0.445 - 1641 0.225 - 1753 0.165 - 1830 0.702 Yes 1951 0.230 - 2121 0.744 Yes 2313 0.740 Yes 2507 0.670 Yes 2550 0.597 Yes 2559 0.218 - 2568 0.317 - 2633 0.738 Yes 2766 0.389 - 2821 0.180 - 2851 0.633 Yes 2878 0.627 Yes 2886 0.630 Yes 2914 0.433 - 2929 0.500 - 3040 0.444 - 3048 0.598 Yes 3055 0.494 - 3097 0.642 Yes 3103 0.530 Yes 3120 0.769 Yes 3180 0.717 Yes 3187 0.358 - 3208 0.474 - 3325 0.574 Yes 3429 0.564 Yes 3458 0.540 Yes 3542 0.375 - 3615 0.517 Yes 3622 0.262 - 3633 0.408 -

Explain the output -- Processing locus 14: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTCAAGTTCATAACTCTTTTTACTGTGTGTTTGAAATTCTGAATTGGTTCTTAGTGTTTCAAGTTTATGCTTATGCATT CGCTTGTATGACTGGTGATGAATGTTTTTGTGTTCATATGATTATGGCATGAATGTTTTATTCCCTCTTTTATCAGAGTT TTGCACTTATTTTTTGTTGTCTTGTCTCTGTGTCAGGAAAAAAATGTCAGGCTCTAGGCCGACTCAGTCCTCTGAGGGCT CAAGGCGATCAAGGCACAGCGCTAGGATCATTGCGCAGACCACTGTAGATGCGAAACTCCATGCTGATTTTGAGGAGTCA GGCAGCTCCTTTGATTACTCAACCTCAGTGCGTGTCACTGGCCCGGTTGTGGAGAATCAGCCACCAAGGTCTGACAAAGT TACCACGACTTATCTTCATCATATACAGAAGGGAAAGCTGATTCAGCCCTTCGGTTGTTTACTTGCCTTGGATGAGAAGA CCTTCAAAGTTATTGCATACAGCGAGAATGCATCTGAGCTGTTGACAATGGCCAGTCATGCAGTTCCTAGTGTTGGCGAA CACCCTGTTCTAGGCATTGGGACAGATATAAGGAGTCTTTTCACTGCTCCTAGTGCGTCTGCATTGCAGAAAGCCCTTGG ATTTGGAGATGTCTCTCTTTTGAATCCCATTCTTGTGCACTGCAGGACTTCTGCAAAGCCCTTTTATGCGATTATCCACA GGGTTACAGGGAGCATCATCATCGACTTTGAACCCGTGAAGCCTTATGAAGTCCCCATGACAGCTGCTGGTGCCTTACAA TCATACAAGCTCGCTGCCAAAGCAATCACTAGGCTGCAATCTTTACCCAGCGGGAGTATGGAAAGGCTTTGTGATACAAT GGTTCAAGAGGTTTTTGAACTCACGGGGTATGACAGGGTGATGGCTTATAAGTTTCATGAAGATGATCACGGTGAGGTTG TCTCCGAGGTTACAAAACCTGGGCTGGAGCCTTATCTTGGGCTGCATTATCCTGCCACCGACATCCCTCAAGCAGCCCGT TTTCTGTTTATGAAGAACAAGGTCCGGATGATAGTTGATTGCAATGCAAAACATGCTAGGGTGCTTCAAGATGAAAAGCT TTCCTTTGACCTTACCTTGTGTGGCTCCACCCTTAGAGCACCGCACAGCTGCCATTTGCAGTACATGGCCAACATGGATT CAATTGCATCTCTGGTTATGGCGGTTGTAGTTAACGAGGAAGATGGAGAAGGGGATGCTCCTGATGCTACTACACAGCCT CAAAAGAGAAAGAGACTATGGGGTTTAGTGGTTTGTCACAATACGACTCCGAGGTTTGTTCCATTTCCTCTCAGGTATGC CTGTGAGTTTCTAGCTCAAGTGTTTGCCATACACGTCAATAAGGAGGTGGAACTCGATAACCAGATGGTGGAGAAGAACA TTTTGCGCACGCAGACACTCTTGTGCGATATGCTGATGCGTGATGCTCCACTGGGTATTGTGTCGCAAAGCCCCAACATA ATGGACCTTGTGAAATGTGATGGAGCAGCTCTCTTGTATAAAGACAAGATATGGAAACTGGGAACAACTCCAAGTGAGTT CCACCTGCAGGAGATAGCTTCATGGTTGTGTGAATACCACATGGATTCAACGGGTTTGAGCACTGATAGTTTGCATGACG CCGGGTTTCCTAGGGCTCTATCTCTCGGGGATTCGGTATGTGGGATGGCAGCTGTGAGGATATCATCGAAAGACATGATT TTCTGGTTCCGTTCTCATACCGCTGGTGAAGTGAGATGGGGAGGTGCGAAGCATGATCCAGATGATAGGGATGATGCAAG GAGAATGCACCCAAGGTCATCGTTCAAGGCTTTCCTTGAAGTGGTCAAGACAAGGAGTTTACCTTGGAAGGACTATGAGA TGGATGCCATACACTCCTTGCAACTTATTTTGAGGAATGCTTTCAAGGATAGTGAAACTACTGATGTGAATACAAAGGTC ATTTACTCGAAGCTAAATGATCTCAAAATTGATGGTATACAAGAACTAGAAGCTGTGACCAGTGAGATGGTTCGTTTAAT TGAGACTGCTACGGTGCCAATATTGGCGGTTGATTCTGATGGACTGGTTAATGGTTGGAACACGAAAATTGCTGAGCTGA CTGGTCTTTCGGTTGATGAAGCAATCGGGAAGCATTTCCTCACACTTGTTGAAGATTCTTCAGTGGAAATCGTTAAAAGG ATGCTAGAGAACGCATTAGAAGGAACTGAGGAGCAGAATGTCCAGTTTGAGATCAAGACACATCTGTCCAGGGCTGATGC TGGGCCAATAAGTTTAGTTGTAAATGCATGCGCAAGTAGAGATCTCCATGAAAACGTGGTTGGGGTGTGTTTTGTAGCCC ATGATCTTACTGGCCAGAAGACTGTGATGGACAAGTTTACGCGGATTGAAGGTGATTACAAGGCAATCATCCAAAATCCA AACCCGCTGATCCCGCCAATATTTGGTACCGATGAGTTTGGATGGTGCACAGAGTGGAATCCAGCAATGTCAAAGTTAAC CGGTTTGAAGCGAGAGGAAGTGATTGACAAAATGCTCTTAGGAGAAGTATTTGGGACGCAGAAGTCATGTTGTCGTCTAA AGAATCAAGAAGCCTTTGTAAACCTTGGGATTGTGCTGAACAATGCTGTGACCAGTCAAGATCCAGAGAAAGTATCGTTT GCTTTCTTTACAAGAGGTGGCAAGTATGTGGAGTGTCTGTTGTGTGTGAGTAAGAAACTGGACAGGGAAGGTGTAGTGAC AGGTGTCTTCTGTTTCCTGCAACTTGCCAGCCATGAGCTGCAGCAAGCGCTCCATGTTCAACGTTTAGCTGAGCGAACCG CAGTGAAGAGACTAAAGGCTCTAGCATACATAAAAAGACAGATCAGGAATCCGCTATCTGGGATCATGTTTACAAGGAAA ATGATAGAGGGTACTGAATTAGGACCAGAGCAAAGACGGATTTTGCAAACTAGCGCGTTATGTCAGAAGCAACTAAGCAA GATCCTCGATGATTCGGATCTTGAAAGCATCATTGAAGGATGCTTGGATTTGGAAATGAAAGAATTCACCTTAAATGAAG TGTTGACTGCTTCCACAAGTCAAGTAATGATGAAGAGTAACGGAAAGAGTGTTCGGATAACAAATGAGACCGGAGAAGAA GTAATGTCTGACACTTTGTATGGAGACAGTATTAGGCTTCAACAAGTCTTGGCAGATTTCATGCTGATGGCTGTAAACTT TACACCATCCGGAGGTCAGCTAACTGTTTCAGCTTCCCTGAGGAAGGATCAGCTCGGGCGTTCTGTGCATCTTGCTAATC TAGAGATCAGGTTAACGCATACCGGAGCTGGGATACCTGAGTTTTTACTAAACCAAATGTTTGGGACTGAGGAAGATGTG TCAGAAGAAGGATTGAGCTTAATGGTTAGCCGGAAACTGGTGAAGCTGATGAATGGAGATGTTCAGTACTTGAGACAAGC TGGGAAATCAAGTTTCATTATCACTGCGGAACTCGCTGCAGCAAACAAGTAGTCCCCAAAAGAAAAGGGGTCTGGCTTGA TATAAAATAGTCACTGGTTGTTCTTTGCTTGTAACTTTCCTTATCGCTTTTGTTTTCGTTTTCAAATTTCA ...................................................................N.....N...... .......N.........N...N...............N.....N....N...N........................... ...........................................i.................................... ................................................N...........N................... ................................................................................ .......................................................................N........ ...........................N...................i.........N...................... ................................................................................ ........N........................................................N.............. .............................................N..........i....................... .........................................................i....................i. .............................N..........i...............N.....N................. ................................................................................ .........N.................i...............i........i.................N......... ................................................................i........i...... .................i........................N...........N........N................ .................i..........................................................N... ................................................................i............... .............................N.....N......N..................................... N.............i....N..............................i............................. .....................N..................i.................................N..... .....................................N......i.............................i..... ...................................i................N........N........N..N...... ....i.....................................................................N....i ...N................................N..........................N................ ................N..............N..................................i............. ......................................N...........N............................. ...............N................................................................ N....................................N......................................N... .......................N...N...................N................................ N.........................N..................................................... ...............................N.........i........................N............. ...............................N..................................N............. ..........................................N..................................... .........................N...................................................... ................................N....................N.......................... .................................................................N.............. N..........................................................N.................... ........N..............................N...............i..................N..... ..........................N..i.................................N................ ...N...............N........................................N.....i............. ................................................................................ ........................................................i..................N.... .....................N..........................N...i.....i..................... ................................................................................ ....................................................................... Pos Score Pred ------------------------ 68 0.068 - 74 0.097 - 88 0.250 - 98 0.181 - 102 0.284 - 118 0.460 - 124 0.369 - 129 0.244 - 133 0.470 - 204 0.650 Yes 289 0.439 - 301 0.491 - 472 0.154 - 508 0.250 - 528 0.792 Yes 538 0.232 - 649 0.361 - 706 0.183 - 766 0.258 - 777 0.693 Yes 858 0.564 Yes 879 0.785 Yes 910 0.340 - 921 0.783 Yes 937 0.262 - 943 0.225 - 1050 0.353 - 1068 0.637 Yes 1084 0.561 Yes 1093 0.582 Yes 1111 0.338 - 1185 0.814 Yes 1194 0.915 Yes 1218 0.569 Yes 1243 0.482 - 1255 0.066 - 1264 0.061 - 1298 0.547 Yes 1357 0.215 - 1425 0.599 Yes 1470 0.358 - 1476 0.462 - 1483 0.218 - 1521 0.435 - 1535 0.686 Yes 1540 0.492 - 1571 0.622 Yes 1622 0.296 - 1641 0.717 Yes 1675 0.167 - 1718 0.403 - 1725 0.720 Yes 1755 0.752 Yes 1796 0.731 Yes 1813 0.344 - 1822 0.168 - 1831 0.106 - 1834 0.192 - 1845 0.760 Yes 1915 0.087 - 1920 0.603 Yes 1924 0.194 - 1957 0.167 - 1984 0.172 - 2017 0.068 - 2032 0.160 - 2067 0.721 Yes 2119 0.081 - 2131 0.179 - 2176 0.263 - 2241 0.499 - 2278 0.344 - 2317 0.122 - 2344 0.151 - 2348 0.498 - 2368 0.106 - 2401 0.093 - 2427 0.253 - 2512 0.363 - 2522 0.668 Yes 2547 0.417 - 2592 0.483 - 2627 0.189 - 2683 0.308 - 2746 0.314 - 2833 0.290 - 2854 0.140 - 2946 0.481 - 2961 0.348 - 3020 0.325 - 3049 0.137 - 3080 0.466 - 3096 0.583 Yes 3115 0.232 - 3147 0.405 - 3150 0.619 Yes 3184 0.111 - 3204 0.472 - 3220 0.247 - 3261 0.431 - 3267 0.604 Yes 3417 0.555 Yes 3436 0.470 - 3462 0.421 - 3489 0.283 - 3493 0.512 Yes 3499 0.617 Yes

Explain the output -- Processing locus 15: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTCACTGTTCTTGAATTATAATTTCTAGGTTTTTTTTATCTTGGCATGTATAAAAATTGAATCTTTATTCTCGTAGGAT GTTATGATTGTGCGGTTTTAGTTGATGTGTTTGGTAATGGGAAAAGGTAGAGTTAAAGCAGTGGAGAAAAGGGTTTTAGA TCAGAAACTTAGAGGATCGATCAATGTTCCTTTAGGACCAGTTTATTATCCGACAGAAGATGAATTCAAAGATCCATTAG AGTATATACACAAGATCAAGCCAGAGGCAGAGGTTTATGGTATTTGCAAGATTGTTCCACCTAATAATTGGAAACCTCCT TTTGGTTTGGATTTGGAATCTGTTAAGTTTCCTACAAAGACACAAGAGATTCATCGGTTGCAGTTTCGTCCTTCTTCGTG TAATTCAAAGACGTTTAAGTTGGAGTACAATAGGTTTTTGGAGGAGCATTTAGGTAAGAAGCTGAAGAAGAGAGTTGTTT TTGAAGGAGAAGAATTGGATTTGTGTAAGCTGTTCAACGCAGTGAAGCGGTTTGGAGGTTATGAAAAGGTTGTCAAAGGG AAAAAATGGGGTGAAGTGTATCAGTTTATGAGCTCTGGTGAAAAGATTTCAAAGTGTGCGAAACATGTCTTGTGTCAGTT ATACAAAGAGCATTTGCATGATTTCGAGAATTATCATAATCGGATGAATGTAGATGCGTCAAAGAGTTGCAAAAGGAAGA TTAATGGCATTCGACGATGTTCTGAATCTCGGAGCTCGAAGAGAAGAAAAAGAAATGCTGATGTGAAAAATCCCAAGGTG GAAAATGAAGAAGGGGTTGATCAAGCGTGTGAGCAATGTAAAAGTGACAAACACGGTGAAGTGATGCTTTTGTGTGATAG TTGTAATAAAGGTTGGCATATATATTGTCTATCGCCACCGTTGAAGCATATCCCACTTGGGAACTGGTATTGCCTTGAGT GTTTAAACACTGATGAAGAGACTTTTGGCTTTGTGCCTGGTAAATGCTTATTACTTGAAGATTTTAAGCGAATTGCTGAT CGAGCCAAAAGAAAGTGGTTTGGTTCAGGAACAGTGTCTAGGACGCAGATAGAGAAAAAGTTTTGGGAGATAGTAGAAGG GTCAGGTGGTGAGGTTGAAGTTATGTATGGTAATGACTTGGATACTTCTGTTTACGGGAGTGGTTTTCCTCGAATAGGCG ACCAAAGACCAGAATCAGTTGAGGCAGATATATGGGATGAGTATTGTGGTAGCCCCTGGAATCTCAATAACATGCCTAAG TTGAAAGGATCTATGCTTCAGGCCATTCGACATAACATTAATGGTGTTACAGTGCCTTGGCTATATCTTGGAATGCTCTT CTCTTCTTTTTGTTGGCATTTTGAGGACCATTGTTTTTACTCTGTCAATTATCTACACTGGGGAGAGGCAAAATGTTGGT ATGGTATTCCAGGCAGTGCTGCTAGTGCTTTTGAAAAGGTCATGCGAAAAACCCTACCCGATCTCTTTGATGCTCAGCCA GATTTGCTCTTTCAACTAGTTACCATGTTGAGTCCGACTGTTTTACAAGAAAATAAAGTCCCGGTCTACACAGTATTACA GGAGCCAGGAAACTTCGTGATCACATTTCCAAAATCCTTTCATGCTGGATTCAATTTTGGTCTAAATTGTGCAGAGGCCG TCAACTTTGCTACTGCTGATTGGCTACCTTATGGTGGTTCTGGGGCGGAGCTGTATCGGCTGTACCGGAAACCTTCAGTC ATATCTCATGAAGAGCTTCTCTGCGTGGTAGCTAAGGGAAACTGCTGCAATAACGAAGGGTCAATACATTTGAAGAAAGA ATTGCTCAGAATATACAGCAAGGAAAAAACCTGGAGAGAGCAGCTCTGGAAAAGTGGTATTTTGAGATCTTCTCCTATGT TTGTGCCTGAATGCGCTGATTCTGTGGGCATCGAAGAGGATCCAACATGCATCATATGCCAGCAGTTTCTTCATCTTTCT GCTATCGTCTGCAATTGCAGGCCATCTGTTTTTGCATGCTTAGAGCACTGGAAGCATCTTTGTGAATGTGAACCTACGAA GTTGCGACTTGAATATCGTTATACCCTTGCCGAGTTAGATATGATGGTACAAGAAGTTGAAAAGTTTGGTGGGTGCAAAA CACAAGAAACCAAAATCTCACAACGGCCGAGTTCAGGCACCAAACGATCTATTGCTTTAAATAAAAAGCAGGAGGGAATG CAAGTGAGTCAGGCACGGCCAGCAGACAAATGGCTTCTTAGAGCATCAAAGGTTCTTGACGCTGCGTTTTCTAGTGTTGA ATATGCCACCCTTTTGAAGGAATCAGAACAGTTTCTTTGGGCTGGATCAGAAATGGACCGTGTACGAGATGTTACAAAAA GTTTGAACAAAGCAAAGATATGGGCTGAAGCTGTTAGTGACTGTCTTTCAAAAGTCGAAGGTGAAGTCAACGATGATTCA ATGAAAGTTCACTTGGAATTTATTGATGAGTTGCTGAGAGTTAATCCTGTTCCTTGCTTTAATTCTGGTTATCTGAAACT AAAGGACTATGCTGAAGAAGCTAGAAAATTGTCCGAGAAAATCGATTCAGCTCTCTCGAGTAGCCCAACTATCACCCAGC TGGAGCTATTGCATTCTGAAGTCTCCAGATCGCCAATCTCCCTAAAAAAACACGAGATCTTGTCAAAGAAAATCTCTTCC GCAAAGATGTTAGCTAAAAGGGCGAAACGCTATCTCACAGACGCTAAACCTCCAGGAATTGAAATGGATGCACTTTTCAA GCTAAATTCAGAGATGTTAGAGCTTCATGTTCAACTTCCAGAAACAGAAGGGATTCTGGATTTGGTAAAGAAATCAGAAT CAGCCCGTGATAAAAGTAACAAAGTTTTGACTGGTTCTTTATCTCTCGAGAATGTGGAAGAGTTGCTTCATGAATTCGAT AGTTTCAGCATTAACGTTCCTGAGTTGAATATCCTGAGGCAGTATCATGTTGACACTTTGTCTTGGATTTCACGCTTTAA TGATGTAATGGTTGATGTTCGTGAAGGCAAGGACCAACGAAAGCTAATTAGTGACCTCAGTTCCCTTCTACGGGATGGAG CATCCTTAGGCATTCAAGTTGAAGGACTCCCTCTTGTTGAAGTTGAATTGAAGAAGGCATCTTGCCGGGAAAAAGCTCGA ACGGTTTATACTGCAAGAAAATCTCTGGATTTCATTGAGCAACTGCTCTCGGAAGCTGTTATACTACACATCGAAGAGGA GGAGATATTTGTTGAGATCTCGGGAATTTTGTCAACAGCACGGTGTTGGGAGGAAAGAGCAAGCACTATCCTTGAAAATG AAACTCAGATGTACGAGCTTAAAGATCTCGTAAGGATGTCAGTCAACATTGATGCGGTTTTACCTACTCTGCAAGGCATA GAGAACACAATCTCGTCGGCTGAAACTTGGCTTCAGAAATCTGAGCCTTTTCTATCAGCCACTTCCTCTATGGCATCATC TCCATGTTCTATGCTTGAACTTCCTGTATTAAAGGATCTAGTTACTCAGGCTAAATTGCTCAATGTTCAGCTTCAAGAGC CACGAATTCTTGAAACATTGTTGCTTAACTGCGAGAGGTGGCAGTGTGATAATCATCAGCTCTTGCAAGAAACTGAAGAT TTGTTGGACAATGCGAAAATAGATGATGGCACGCATAGCAATATCCTTCCGAAGATAATGGATTTGATAACCAGAGTGGA CTCCGCTAGGAGATCTGGTCTGGCCCTTGGTCTCAATTTCGATGAACTTCCCAAACTTCGAACTGCAAGTCTAAAACTAG GATGGTGTTGTAAGACCATCACGTTAAGCTCTAGCTCACCTACCTCTGAGTTGCTAGAAGATGTTGGAAAGCCCTCGTTA CAGCATATTCAGCAGCACTTAAAAGAGGGACAAACACTTGAAATATTACCTGAAGAGTATTACTTAGGCAAGAGACTCAT GGAGTTAAAAGACACTGGACTGGAGTGGGCAAAACGAGCTAGAAAGGTGGTAACAGACTCAGGTGCTCTTGCCTTGGAAG ATGTTTTCGAGCTTATTTCTGAGGGTGAAAACTTACCTGTTCATGCAGAGCAGGAACTTCAGTCTTTACGAGCTCGGAGT ATGTTACACTGCATTTGTCTAAAGCCATACAACTCAAGATCCATGGTTTCTTGTAGTCAATGTGGCGAATGGTATCACAC CTATTGTTTAAAGCTTCATTGGCGGCCTAAGGCTTATGTCTGCTCCGCTTGCTGTCCACTGGCAGAAACCACTCCACAGA TCGATCCCGCCAGAGCAACAGAGCCAGAGAGACCGTCTCTGAACCAAAGACGGACAAGAATGGTCGCGACTGATGCAGCA GTTAATGACTTAAAGTGGAAAACCCGAAAACACATCAAACGGACAACTAAACGGAGTCCTCAGGTTCATATTCTTCCCTG GTTTTTCACTTAAAAACCAACATAATAATCTCTTGAGGGTATGATATATAGAGCAATTTCATTTATTCAATTTTAGATGA GTTTTCTCAAACCTTTTTGTAATGTTTCCCAT ..............................................N...............................N. ...N....................N...........N........................................... .......................N...................................N.................... ....................................N........................................... ................................................................................ ................................................................................ ............................................................N................... .....i.....................N....................................N............... .................N.........................N...i.....N.......................... ...i............N.....................................N.....N................... ....N..............................N...........................N................ ................................................................................ ............N..............................N.................................... ................................................................................ ......................i...N.....i............................................... ...............................N....N..................................N........ ............N...........................N...............................N....... ........................................................................N....... N........................................N...........................N.......... ........................i....................................................... .........................................N...................................... ..............................N................................................. .......N........................................................................ ............................................................................N... ..........N...................................N........N........................ ...................................N.............................i.............. ........................................N..i.................................... .............................................................................i.. .............................i.................................................. ..N.................................................i...............N........... ...................N....................................................N....... i........................N...................................................... ........N....................................................................... ................................................................................ ......i........................................................i...N............ .............N............N..................................................... ...................................................N.................N.......... ..............................................N................................N ..N....i......N...........................................................N..... ................................................................................ ................................................................................ .............................................................................N.. ........i..........................N...............N............................ .....................................................................N.......... ...N......N...................................................N................. ................................................................................ ..........N...........N..N...............................N...................... .........................................N...................................... ..............................................................................i. ................................................................................ N.........................................N..................................... N.........................................N................N........i........... ...................................i............................................ ...........................................................N............N....... ....N........................................................................... ........................................N...................................N... .....................N.......... Pos Score Pred ------------------------ 47 0.350 - 79 0.195 - 84 0.421 - 105 0.313 - 117 0.390 - 184 0.097 - 220 0.076 - 277 0.227 - 541 0.150 - 566 0.510 Yes 588 0.244 - 625 0.224 - 658 0.357 - 684 0.471 - 688 0.532 Yes 694 0.356 - 724 0.589 Yes 737 0.389 - 775 0.309 - 781 0.219 - 805 0.347 - 836 0.147 - 864 0.118 - 973 0.349 - 1004 0.360 - 1143 0.671 Yes 1147 0.131 - 1153 0.507 Yes 1232 0.283 - 1237 0.132 - 1272 0.182 - 1293 0.147 - 1321 0.223 - 1353 0.201 - 1433 0.493 - 1441 0.303 - 1482 0.309 - 1510 0.186 - 1545 0.607 Yes 1642 0.266 - 1711 0.366 - 1768 0.276 - 1917 0.380 - 1931 0.109 - 1967 0.491 - 1976 0.249 - 2036 0.369 - 2066 0.514 Yes 2121 0.349 - 2124 0.567 Yes 2238 0.537 Yes 2270 0.551 Yes 2323 0.348 - 2373 0.813 Yes 2389 0.139 - 2420 0.433 - 2473 0.180 - 2481 0.647 Yes 2506 0.213 - 2569 0.348 - 2727 0.725 Yes 2784 0.683 Yes 2788 0.232 - 2814 0.447 - 2827 0.149 - 2932 0.204 - 2950 0.263 - 3007 0.206 - 3040 0.181 - 3043 0.294 - 3048 0.582 Yes 3055 0.371 - 3115 0.293 - 3358 0.193 - 3369 0.547 Yes 3396 0.369 - 3412 0.129 - 3510 0.193 - 3524 0.376 - 3531 0.316 - 3583 0.260 - 3691 0.237 - 3703 0.144 - 3706 0.226 - 3738 0.261 - 3802 0.193 - 3842 0.610 Yes 3901 0.270 - 3999 0.708 Yes 4081 0.226 - 4123 0.151 - 4161 0.196 - 4203 0.241 - 4220 0.270 - 4229 0.582 Yes 4276 0.546 Yes 4380 0.499 - 4393 0.181 - 4405 0.295 - 4521 0.039 - 4557 0.053 - 4582 0.055 -

Explain the output -- Processing locus 16: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTGATTATTCGGATGAAGCTAACATCTTTCTGTATAATGGTGTCTTTACGCAGTCTCCTTTGTCTGGCTTTGAAAGTT TGGTATATTTATAATTAGTAACGAATGGGAAAGAGATTAAAGAAGGGTCAAAAGCCTCCATATATGAGGTTTGCTGCAAC GGTCTCCGAGAAGCAATCTGAGCAAAGCGTGGAAGTAACTGAGGAGTTTGCTGTGACAATGACAGAAAAAAGATCTTGTG TCCATTTTGATAAATATGTTGATTTGGATAAGTTGTTGAAAATTATCAAATCTTATCAACAAATCAAGTGTGGAGAATGC AATGAAAGAGTTCACGTGAAGAGAGGAAGTAGATGGGGTGCTAGTAACAGGAGAGACTGGTATTCTTCCTCTGACCAGAA TTGTGCTAGAAATGCTATTTGGCTTTGTTTGGAATGTGGCTATTATGTTTGTGGAGATGTTGGTTTGCCGACTGAGGCTC AGAGTCATGTTATGGGGCATAACAGGTTGACGCGTCACCGTTTGGTGATTCAATGTAAAAATCCTCAGTTAAGGTGGTGT TTCTCATGCCAATCACTTCTCCCTTTTGATAATGAAGAGAATGGTGAGAAAAAAGATTTGTTGCTGGAAGTTGTGAAATT GATTAGAGAAAGATCTCCAAACACGTACTCTGCTTCATTTGAAACTGAATTTTCATGTTCAGGAAGTATCTATGGTGGTA TAGAAGCAAGAGATGGTTATGCTGTAAGAGGTTTAGTTAACCTTGGGAACACATGTTTCTTTAATTCTGTAATGCAGAAC CTTCTCTCTTTGGATCAATTACGTGAGCACTTCTTGAAAGAGGATCTATCTGTTAGCGGGCCTCTCGTTTCTTCCCTGAA GGAACTATTTGCTGAATCAAATTCAGAGGCAAGCGTTTTTAGGAATGAGATAAATCCAAGAGACCTTTTCTTTTCTGTCT GTTCTCAGGCTCCCCAATTTAGGGGATATCAGCAGCATGACAGTCATGAGTTGCTAAGATGTTTGTTGGATGGTTTGAGC ATTGAAGAATCGAGCCTGAGGAAGAAGCTTGGTGTTTCTGATAGTAATGACAGTTCTACATATCAGAAACCTACTCTCAT AGATTCTGTATTTGGAGGTGAAATATCGAGCACTGTTTCTTGTTTAGAATGCGGACATTTCTCAAAAGTGTATGAACCCT TTATGGATCTCTCTTTACCCGTTCCGTCCATGAAACTTCCTCCTAAAAAGCAACAAATCCTTTCTCAGGCAAAGGAGGTC CTTAAGAATGGGGCAGTCAGCAAAGATTCAGAAGTTGTGTCCGCTAAACCTGCATCGGATCACAATTCCACAGTTCCACT CTTTCCATCGGATCACAAGATTCAAAGCAGACCAGAAACCTCTGACAATGAAACCGATTTGGTTTTGCTAAGCGATGTTA GTGATACTGCCCCATCAACCGAGGCTAAAGGAGTTAATCAGATTTTGGTAGGTAGCACAGAAACGCTGATGCATGATAAT GATCGAACTGGAAAAACTGTACCAGATAAAGAAGATGTACGAGCTACGCAGTCTAATGAAGAAACATCTGCAAGTGGGAT TTCAGCAGTGATAGATGAGGCTCAAGTTTGCGGTTGTCCTGATTTAGAGCAAAGTTCTTCATCTGCAAATCAGGGGGCTG ATGAAGAGCTTGCCTTGATGGTTGCAGATTCTCAGGTTCTGTATATGCCTTATAAAGATCACTTATTCTATGATGATTAT ATGGTTGCAGAAGCTTCCTCGTCATTCGTGAGCGGTGACCATGAACCAAAAAAAGACTATTTTGACTTTAGCAGTTTTCT TGATGAGCCTGAGATACGCGAAGGACCTGTTTTTAGACCACTATCTAAATCTGAAGTTTATGAAGCTGGTTTTAAGGCTG ACTGCAGTGATGATAAAACAGTTAGTGCAGGTAAGGGTGAAGCTTCCTCGTCATTCATAAGTAGTGACCATGAACAAAAC ATAGATTATGTTGACTTTAGCAGTTTTTTTGACGAGCCTGAGATATCCGAAAGACCTTTCTTTCGACCGCTATCTAAATC TGAAGTTTCTGAAGCTGGTTTTAAGGCTGACTGCAGTGATGATAAAACAGTTAGTGCAGGTAAGGGTGAAGCTTCCTCGT CATTCGTAAGCAGTGACCATGAACAAAACATAGATTATGTTGACTTTAGCAGTTTTTTTGATGAGCCTGAGATATCCGAA AGACCTTTCTTTCGACCGCTATCTAAATCTGAAGTTTCTGAAGCAGGTTTTATGGCTGTCAGCGGTAATGATAAAACAGT TAGGGCAGGTAAGGGTGAAACTTTTTCTTCATTCATGAGCGGTGACAATGAACGAAACATAGATTATGTTGAGTTTACTA ACAGAATATTCGATGATCGTGGGACATCTGAAAGACCTGTTTTTGGACCACCATCTAAAGCTAAAGTTTCTGAAGCTGGT TTTGTGGCTGTAAGCAGCGACTCTGATCCTGCAGTTCTTGATGAATCGGATTCACCAGTGTCTGTAGATAGGTGTTTGGC TCAGTTCACGAAGCATGAGATTTTGTCAGAAGACAATGCTTGGCATTGTGAGAATTGTTCGAAGAACCTAAAACTTCAGC GGTTGAGAGAAAAGAGAAGAACTAAAGAAGGATTGAGCAACAGATGGGTGAATGAAAATGGAGCTAGTTCGGCTTTTGAT GAGTGTAGAGACAGTTCACTAAATCAATCCTGTATAGATCTCGAAAACGGTTACAAAGCTGCTCCTCCGATCACAAAATT GCCAAACTGCAAGGAAGAGGAAAGCGCAATAGATGATGGATTTGTGGGAGAGGAAAATACAAAGCAAGCTCCAATTACTT CTGTTACTGAAACACCTCTCCTCGGTGGAGAAACCATCAGTTCTCAACCAGCCAGTGACAATGAATGTGAAAATTGGGAA GACTTGGCAGTAGATTCTGAAGAAGTGATAGTAAAAAGAGATGCTAGGAAAAAGGTACTTATAAACAAGGCACCACCTGT CTTGACCATACATCTCAAAAGGTTCAGCCAAGATGCTCGAGGCAGGGTAAGCAAGCTAAGTGGCCATGTTGATTTCCAAG AGTTCATCGATCTTAGCAAATACATGGACACTAGGTGCTCCGAAGAAGACGAGCCGGTTTACAGGTTGGCCGGGTTAGTG GAACATTTAGGGGCAATGAGTCGAGGTCATTATGTTTCTTACATTAGAGGAGGCCACAAGGAGAGGAGAGACTCTGATAC TAAAGAACCAAACTCATCCATATGGTATCACGCAAGCGATTCTCAGGTTCGACCGGCTTCTTTGGAAGAAGTTCTCCGTT CTGAAGCCTATATCTTATTTTACGAACGAATCTGAGAACACGGAGGCTGGATATCTTTTTTTTTTTTTTTTCTCTTTCTA TAATTTAGGTTAATAGAACAAGATAGGTTTAACGTTCTTCACACATTGTGCTGG ..............N.......................i......................................... ........................i......................................i................ ..........................................................i..................... ...............N............................................................N... ...........N.....................N..........N...........N....................... ......N....N........................................N........................... .....N.........................N........N....................................... ......................................................N................N........ ............N.....N.................................N..................N........ ................................................................................ ............................................N................................... ....................................N........N............N..........N.......... ..............................................N................................. ................................................N......................N........ ..i..........................N.................................................. .......i........................................................................ ...............................................N..........................N..... ....................................................................i...N.....N. ..................................N....................N........................ ..............N................................................................. N................i..........................i........................N..N....... i.......................................N....................................... ..N........................................................N.................... .........N...........................................................N.......... .......i........................................................................ ......................................N......................................... ..................N.................N.......................N................... ...................................................i...............N............ ..................................N............N.................N.............. ............N................................................................... ........................................N....................................... ..............N....................i............................................ ...........................................N.......N.....i....................N. ................................................................................ ................................N..N............................................ ............................................................N...N............... ........................................N....................................... ................................i................................N.............. .......................N........................................................ ...............i...............N................................................ .....................N.......................................................... ................................................................................ ...................................................... Pos Score Pred ------------------------ 15 0.203 - 39 0.517 Yes 105 0.692 Yes 144 0.645 Yes 219 0.596 Yes 256 0.388 - 317 0.164 - 322 0.384 - 353 0.094 - 412 0.119 - 434 0.301 - 445 0.206 - 457 0.307 - 487 0.128 - 492 0.375 - 533 0.205 - 566 0.260 - 592 0.161 - 601 0.294 - 695 0.122 - 712 0.342 - 733 0.263 - 739 0.222 - 773 0.218 - 792 0.405 - 925 0.298 - 997 0.223 - 1006 0.106 - 1019 0.323 - 1030 0.337 - 1087 0.269 - 1169 0.321 - 1192 0.192 - 1203 0.759 Yes 1230 0.439 - 1288 0.759 Yes 1408 0.496 - 1435 0.300 - 1509 0.627 Yes 1513 0.108 - 1519 0.367 - 1555 0.423 - 1576 0.213 - 1615 0.133 - 1681 0.070 - 1698 0.702 Yes 1725 0.585 Yes 1750 0.164 - 1753 0.160 - 1761 0.826 Yes 1801 0.417 - 1843 0.194 - 1900 0.238 - 1930 0.071 - 1990 0.270 - 2008 0.609 Yes 2119 0.088 - 2179 0.294 - 2197 0.493 - 2221 0.168 - 2292 0.850 Yes 2308 0.135 - 2355 0.287 - 2368 0.298 - 2386 0.280 - 2413 0.436 - 2521 0.192 - 2575 0.308 - 2596 0.664 Yes 2684 0.232 - 2692 0.394 - 2698 0.509 Yes 2719 0.215 - 2833 0.267 - 2836 0.175 - 2941 0.404 - 2945 0.315 - 3001 0.255 - 3073 0.705 Yes 3106 0.316 - 3144 0.304 - 3216 0.659 Yes 3232 0.225 - 3302 0.463 -

Explain the output -- Processing locus 17: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGATTCCAATCTTCTCTCGTCTGCTTTCCTCAAAGGTACGTTTTATCTTCCTTCTCATAGCTTCCGAAGAATCAATCTAG GGTTAGGTATTTCAATTCTGATCGGATTTTCTCTCTTCAGTGTTCTCTAATCTAATGGGAATTTCACATGTTTTCTCCAG GTTTTTTCATCACTTTATGCTTCACAAGCGAGCTAGTGAAGCTAACGATAAGAACGATAATACCATCATCGGTTCCGATT TAGCATCATCTAAGAAACGCCGTATCGATTTCACTGAATCTTCGTCTGATAAGTCTTCTTCGATTTTAGCTTCTGGTAGT AGCAGGGGTTTTCACGGCGATAGCGTCGTTCAGCAAATCGACATGGCTTTTGGTAATTCGAACCGTCAGGAGATTGATGA AGATCTGCACAGTCGCCAGCTCGCCGTCTATGGTCGTGAGACTATGAGGCGTCTCTTTGCTTCCAATGTTCTCATCTCGG GGATGCACGGTCTTGGTGCCGAGATTGCCAAGAATCTTATACTTGCTGGTGTGAAGTCTGTGACCTTGCATGATGAAAGA GTTGTAGAGCTATGGGACTTATCAAGCAACTTTGTTTTCTCGGAGGATGATGTTGGCAAGAATAGAGCAGATGCTTCTGT TCAGAAGTTACAGGATCTTAACAATGCTGTGGTTGTCTCTAGCTTGACCAAAAGCTTAAACAAAGAGGATCTTTCTGGCT TCCAGGTTGTTGTATTCTCTGACATAAGCATGGAAAGAGCAATCGAATTTGATGACTATTGTCACAGCCATCAGCCTCCT ATCGCTTTTGTCAAGGCTGATGTCAGGGGTCTTTTCGGCTCTGTCTTTTGTGATTTTGGGCCTGAATTTGCGGTTCTTGA TGTTGATGGAGAGGAACCCCATACAGGCATCATTGCGTCTATCTCTAATGAGAACCAGGCATTTATTTCCTGTGTCGACG ATGAGAGGCTTGAGTTTGAAGATGGTGACCTAGTTGTTTTCTCAGAAGTTGAGGGTATGACAGAATTGAATGATGGGAAA CCAAGGAAGATAAAAAGTACACGGCCATATTCATTCACCCTTGATGAGGACACTACTAACTATGGAACATATGTGAAGGG TGGTATTGTCACTCAAGTGAAACAGCCAAAGTTACTGAATTTCAAGCCATTGAGGGAAGCGCTTAAGGATCCAGGGGATT TTCTATTTAGTGATTTCTCTAAGTTTGATCGGCCTCCACTCCTTCACTTAGCATTCCAGGCACTTGATCATTTTAAAGCT GAAGCTGGCAGATTTCCTGTTGCTGGCTCAGAAGAGGACGCTCAGAAGCTTATATCGATTGCCACAGCCATCAATACCGG GCAGGGTGATTTAAAGGTGGAGAATGTTGATCAGAAGCTTCTAAGACACTTTTCCTTTGGAGCCAAGGCTGTCCTGAATC CTATGGCTGCAATGTTTGGCGGTATTGTTGGACAGGAGGTTGTTAAAGCTTGCTCTGGAAAATTTCATCCTCTCTTCCAG TTTTTCTACTTTGATTCAGTGGAGTCACTCCCCTCTGAACCTGTGGACTCGAGTGACTTTGCACCAAGAAACAGCCGGTA TGATGCCCAAATATCTGTATTTGGGGCCAAGTTCCAGAAGAAACTTGAAGATGCTAAAGTTTTCACAGTAGGGTCTGGTG CTCTTGGCTGTGAGTTCTTGAAAAATTTGGCTCTGATGGGGGTTTCTTGTGGAAGCCAAGGGAAGCTGACAGTGACAGAT GATGATATCATTGAGAAGAGTAACCTCAGTCGTCAATTTCTGTTCCGTGACTGGAACATTGGACAGGCTAAATCCACAGT TGCTGCTTCGGCTGCTGCTGTTATAAATCCTCGCTTCAACATTGAGGCCCTGCAAAACCGTGTTGGCGCGGAGACTGAGA ATGTATTTGACGATGCCTTCTGGGAGAACTTAACTGTTGTCGTCAATGCACTGGATAATGTTAATGCGAGGCTCTATGTT GATTCGAGGTGCTTGTATTTCCAGAAGCCTCTCCTTGAGTCTGGAACTCTTGGTACAAAGTGCAACACACAGTCGGTTAT CCCACATCTAACTGAAAATTACGGTGCCTCAAGGGACCCACCAGAGAAACAGGCCCCCATGTGTACAGTGCATTCCTTCC CGCATAACATTGATCACTGTTTAACTTGGGCTCGCTCTGAGTTTGAGGGTCTGCTGGAGAAGACTCCCGCTGAAGTGAAT GCATATCTTTCTAGCCCAGTTGAGTACACTAACTCAATGATGAGTGCTGGTGATGCTCAGGCGAGGGACACATTGGAGAG GATTGTTGAGTGCCTTGAAAAGGAGAAGTGTGAGACCTTCCAAGACTGCTTAACCTGGGCACGACTGAGGTTTGAGGATT ACTTTGTGAACCGTGTCAAGCAATTGATATACACATTTCCTGAAGATGCTGCGACTAGTACCGGAGCTCCATTCTGGTCT GCTCCAAAAAGATTCCCTCGTCCACTCCAGTACTCGTCGTCCGACCCAAGTCTCCTTAACTTTATCACAGCGACTGCTAT TTTACGAGCAGAGACATTTGGGATCCCGATACCTGAGTGGACTAAGAACCCAAAGGAAGCAGCAGAAGCTGTTGACAGAG TGATAGTCCCAGACTTTGAGCCAAGGCAAGATGCAAAGATTGTGACTGATGAGAAAGCTACCACTTTAACCACTGCTTCG GTGGACGACGCTGCAGTCATCGACGATCTCATTGCTAAGATCGACCAGTGTAGGCATAACTTGTCGCCAGACTTCAGGAT GAAACCTATTCAGTTCGAAAAGGATGATGATACAAACTATCACATGGATGTGATAGCAGGACTAGCCAACATGAGGGCGA GAAATTACAGCATCCCTGAAGTGGACAAGCTGAAAGCAAAGTTCATAGCAGGGAGAATCATCCCAGCCATTGCGACTTCA ACAGCCATGGCTACTGGGCTGGTCTGCCTCGAGCTGTACAAGGTCCTTGATGGAGGACATAAAGTGGAAGCTTACAGAAA CACATTTGCCAACCTGGCACTTCCACTATTCTCCATGGCTGAACCACTTCCACCAAAGGTGGTGAAGCACCGTGACATGG CTTGGACAGTTTGGGACAGATGGGTTCTCAAGGGAAACCCAACTCTTCGTGAGGTGTTGCAGTGGCTGGAGGACAAAGGG CTTAGCGCATACAGCATCTCTTGTGGAAGCTGTCTTCTGTTCAACAGTATGTTCACAAGGCACAAGGAGAGGATGGACAA GAAGGTTGTGGATCTCGCTCGGGATGTTGCGAAAGTCGAGTTACCGCCTTATCGCAACCATCTTGATGTAGTGGTGGCTT GTGAGGATGAAGATGACAATGACGTCGATATTCCTCTCGTCTCTATCTACTTCAGGTGAAAAGTTTCGAGAGAATGAAGA AAGATGAAACTCTTGAGAGCAAATTTGTGTGTTTTGTCTCTGCATCTGCCCTACGTTATTATCTTTTGGATGCTTGTG ................................................................................ ......................................................i............N............ ................i............................................................... ................................................................................ ..........................................i.................................N... .............................N.............i.....................N.............. ..i..................................................................N..N....... ...........N..................................N..N....................N......... .......................N........................................................ .............................i.....................N............................ ...................N...........................................................N .....N.........................................N................................ N....................N..................................N............N..N....... ...........................................N.................N........N......... ................................................................................ ................................................................................ ................................................................................ .......................N........................................................ ..i........N.................................................................... ...............................................................................N ..N...............................................N............................. ...................................i..........................................N. ................................................................................ N...........N................................N...........N.....N...........N.... ................................................................................ ..........................................................i..................... ..............................................................................N. ....................................i..i............N........................... ................................................................................ .............................................N.................................. ................................................................................ ................................................................................ ..............................N.................N............................... ..............................................................................N. .......................N..N................i...N......................i......... ................................................................................ ......i..........................................N.............................. ..................................i.........................................i... ...................i............................................................ ................................................i.......................i....... .......................N.........................................N.............. ......N.....N.....N......................................................N...... ...N.................................................................N........ Pos Score Pred ------------------------ 135 0.749 Yes 148 0.355 - 177 0.552 Yes 363 0.680 Yes 397 0.244 - 430 0.217 - 444 0.798 Yes 466 0.136 - 483 0.536 Yes 550 0.122 - 553 0.214 - 572 0.234 - 607 0.200 - 610 0.120 - 631 0.250 - 664 0.200 - 750 0.625 Yes 772 0.243 - 820 0.321 - 880 0.113 - 886 0.184 - 928 0.195 - 961 0.132 - 982 0.204 - 1017 0.456 - 1030 0.149 - 1033 0.197 - 1084 0.180 - 1102 0.162 - 1111 0.135 - 1384 0.170 - 1443 0.518 Yes 1452 0.497 - 1600 0.356 - 1603 0.282 - 1651 0.221 - 1716 0.657 Yes 1759 0.323 - 1762 0.096 - 1921 0.261 - 1933 0.307 - 1966 0.255 - 1978 0.281 - 1984 0.183 - 1996 0.231 - 2139 0.768 Yes 2239 0.106 - 2277 0.852 Yes 2280 0.767 Yes 2293 0.281 - 2446 0.447 - 2671 0.179 - 2689 0.227 - 2799 0.444 - 2824 0.285 - 2827 0.210 - 2844 0.841 Yes 2848 0.132 - 2871 0.732 Yes 2967 0.678 Yes 3010 0.326 - 3075 0.536 Yes 3117 0.659 Yes 3140 0.599 Yes 3249 0.571 Yes 3273 0.671 Yes 3304 0.137 - 3346 0.145 - 3367 0.165 - 3373 0.282 - 3379 0.359 - 3434 0.181 - 3444 0.120 - 3510 0.029 -

Explain the output -- Processing locus 18: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGACATGTTTGGCTTTCAGATTGCTTCATTGGAAAGTCAATACAAATCAAATGGGTTTCTGCATCAGATCACGGCAAGTG CAGGTTAAGAATCTGTGATAACCTTTCATTGTGATTACTGATTCAGTCCTTTTGATGATTTCTTATTTTCTTAACCAAGA CTTTTCAAGGAAGAAGCAAGGAAGAATGGCTGCTTCAGGACCAAAAAGCTCAGGTCCCAGAGGTTTTGGGCGACGAACAA CAGTAGGAAGTGCTCAGAAAAGAACTCAGAAGAAGAATGGTGAAAAAGATAGTAATGCCACTTCTACAGCAACAAACGAG GTTTCAGGGATTAGTAAGTTGCCCGCAGCTAAAGTGGATGTACAGAAGCAAAGCTCTGTTGTTTTGAATGAGAGAAATGT GTTAGATAGGTCGGATATTGAGGATGGAAGTGATCGTTTGGACAAGAAAACAACCGATGATGATGATTTGTTAGAACAAA AGTTAAAACTTGAAAGAGAGAATCTTCGTAGGAAGGAAATAGAAACGCTTGCAGCGGAAAATTTGGCGAGAGGTGATAGA ATGTTTGTGTATCCCGTTATTGTGAAACCTGATGAAGACATAGAAGTGTTTCTCAACAGGAATCTGTCGACTCTGAATAA CGAACCCGATGTTTTGATCATGGGGGCGTTTAACGAATGGAGATGGAAGTCTTTCACAAGGAGATTGGAAAAGACCTGGA TCCATGAAGATTGGTTGTCATGTCTCCTTCATATCCCCAAAGAAGCGTATAAGATGGACTTCGTGTTTTTCAATGGGCAA AGTGTATATGACAACAATGACTCAAAAGATTTTTGTGTAGAGATAAAAGGTGGGATGGATAAAGTTGACTTTGAGAATTT TCTTCTAGAAGAGAAACTGCGAGAGCAAGAGAAGTTAGCCAAGGAAGAAGCTGAGAGGGAGAGGCAAAAAGAAGAGAAGA GAAGAATCGAAGCTCAAAAGGCTGCAATTGAAGCTGATAGAGCACAAGCAAAGGCGGAGACTCAGAAGAGACGTGAATTG CTTCAACCGGCTATTAAGAAAGCTGTAGTCTCGGCTGAGAATGTTTGGTACATTGAGCCGAGTGATTTCAAGGCTGAAGA TACAGTGAAGCTATATTACAATAAAAGGTCAGGTCCTCTGACTAATTCCAAAGAACTGTGGTTACATGGAGGGTTTAATA ATTGGGTTGATGGATTATCTATCGTTGTAAAGCTTGTTAATGCTGAGTTAAAGGATGTTGATCCAAAGAGCGGAAATTGG TGGTTCGCTGAAGTTGTAGTGCCTGGCGGTGCACTAGTCATTGACTGGGTCTTTGCTGATGGACCACCTAAAGGAGCGTT TCTGTATGACAATAATGGTTACCAAGACTTCCACGCACTTGTTCCTCAAAAACTTCCTGAAGAACTTTACTGGTTAGAGG AAGAAAATATGATTTTTAGAAAACTTCAGGAGGATAGGCGGTTAAAAGAGGAAGTTATGCGTGCCAAGATGGAAAAAACA GCTCGCTTGAAAGCTGAAACTAAGGAAAGAACACTGAAAAAGTTTCTGCTATCCCAGAAAGACGTGGTTTACACCGAGCC TCTAGAGATTCAAGCAGGAAACCCTGTGACTGTATTGTACAATCCTGCAAACACGGTTTTGAATGGAAAACCTGAAGTTT GGTTTAGAGGCTCTTTTAATCGTTGGACTCACCGCTTGGGCCCTTTGCCACCTCAGAAAATGGAAGCAACAGATGATGAA AGCTCACATGTGAAGACTACGGCTAAGGTCCCATTGGATGCTTACATGATGGACTTTGTGTTCTCTGAGAAAGAGGATGG CGGAATATTTGATAACAAAAATGGTCTGGATTACCATTTACCAGTCGTGGGAGGTATTTCAAAGGAACCACCATTGCACA TTGTTCATATTGCTGTTGAAATGGCACCCATCGCAAAGGTTGGTGGCCTAGGTGATGTTGTCACTAGTCTATCTCGCGCT GTTCAAGAATTAAACCATAATGTGGATATAGTTTTTCCAAAGTATGATTGCATAAAGCACAATTTTGTGAAGGACTTGCA ATTTAACAGAAGCTATCACTGGGGAGGAACTGAAATAAAAGTTTGGCATGGAAAAGTAGAAGGCCTTTCGGTTTACTTCT TAGATCCACAAAATGGATTGTTTCAGCGAGGATGTGTTTACGGTTGTGCAGATGATGCAGGAAGATTCGGTTTCTTCTGT CATGCGGCTCTTGAATTTCTTCTCCAAGGAGGTTTCCATCCAGACATTCTTCACTGTCATGACTGGTCTAGTGCTCCGGT TTCATGGTTATTCAAGGATCATTACACACAGTACGGTTTAATTAAAACCCGTATTGTCTTCACAATTCATAATTTGGAAT TTGGAGCGAATGCCATTGGTAAAGCAATGACATTTGCAGACAAAGCCACAACGGTTTCACCAACTTATGCTAAGGAAGTT GCTGGAAACTCTGTAATCTCTGCACATTTATACAAATTTCACGGAATTATAAACGGGATTGACCCAGATATATGGGATCC ATATAACGATAACTTTATTCCCGTACCTTATACTTCAGAGAACGTTGTAGAAGGCAAAAGAGCAGCCAAGGAAGAATTGC AAAACAGGCTTGGACTAAAGAGTGCCGATTTTCCAGTAGTAGGAATTATTACGCGCTTAACACACCAGAAGGGAATACAT TTGATCAAGCACGCTATTTGGCGTACCTTGGAACGGAATGGACAGGTTGTCTTATTAGGTTCAGCTCCAGATCCTCGGAT CCAAAATGATTTTGTAAACTTGGCAAACCAATTACATTCTTCTCATGGTGACCGGGCTCGGCTTGTTCTAACCTACGATG AACCTCTTTCCCATTTGATTTATGCTGGGGCTGACTTTATTCTTGTACCGTCGATATTTGAGCCATGTGGACTGACACAG CTCATAGCCATGAGATACGGCGCTGTTCCTGTTGTTAGAAAAACTGGAGGACTCTTTGATACGGTTTTTGATGTTGACCA CGATAAAGAAAGGGCACAAGCTCAAGTTCTAGAACCTAATGGTTTCAGCTTCGACGGAGCTGATGCTCCTGGTGTTGATT ATGCTCTCAATAGGGCGATATCGGCGTGGTACGATGGTAGAGAGTGGTTTAACTCGCTGTGCAAGACGGTGATGGAGCAA GACTGGTCATGGAACCGTCCTGCACTTGAGTATCTTGAGCTCTATCACTCTGCACGCAAGTAAGACCAAATAAAATGGAA GCTCTAAGATGAATTCTCTTTGTATGTACAGAAATTAGCTTATGAGACAACTTAGAGAGTTTGATCCTTTCTTCTTCTTT TA ....i.............................................N............................. ......................................................N......................... .........................i...................................................... ....................................N.................N......................... .....................................N.............................N........i... .......................N................................N..N..N................. ................................................................................ N..............................N................................................ ........N..........N................i.....N..................................... ...N...............N.................................i..................N....... .......i........N.....................................i......................... ................................................................................ ................................................................................ ........................................N....................................... .................................................................N.............. .........N.............................N..............N......................... ..........................................................N..................... .....N........N................................................................. ........i...............................................i...........i........... ................................................................................ ..............................................................N................. ...........................................................i............N..N.... .......N.............................N.......N..i...........................N... ....................N........................................................... ....................i.................................N......................... ...................N.......................i.................................... ...............................................N................................ ............i..................N...................N..N......................... ...N............................................................................ .........N................i.......................................N............. .......................................................................i........ ................................................................................ ................................................................................ .....................................N.......................................... .....N......................................N................................N.. .....................N..........................................i............... .........i............................................................N......... ......................................N.......................N................. N................................N.....................................N........ ........N.................................................................N..... ........N..............N.................N...................................... .. Pos Score Pred ------------------------ 5 0.616 Yes 51 0.248 - 135 0.326 - 186 0.789 Yes 277 0.229 - 295 0.186 - 358 0.221 - 388 0.163 - 397 0.670 Yes 424 0.251 - 457 0.134 - 460 0.148 - 463 0.170 - 561 0.493 - 592 0.114 - 649 0.214 - 660 0.268 - 677 0.676 Yes 683 0.494 - 724 0.465 - 740 0.121 - 774 0.704 Yes 793 0.107 - 808 0.504 Yes 817 0.363 - 855 0.787 Yes 1081 0.160 - 1186 0.177 - 1210 0.269 - 1240 0.235 - 1255 0.410 - 1339 0.244 - 1366 0.218 - 1375 0.381 - 1449 0.781 Yes 1497 0.638 Yes 1509 0.538 Yes 1663 0.317 - 1740 0.757 Yes 1753 0.148 - 1756 0.235 - 1768 0.388 - 1798 0.358 - 1806 0.498 - 1809 0.626 Yes 1837 0.120 - 1861 0.316 - 1941 0.802 Yes 1975 0.305 - 2020 0.164 - 2044 0.566 Yes 2128 0.484 - 2173 0.627 Yes 2192 0.193 - 2212 0.446 - 2215 0.421 - 2242 0.345 - 2299 0.181 - 2324 0.363 - 2410 0.110 - 2427 0.632 Yes 2467 0.180 - 2552 0.506 Yes 2758 0.493 - 2806 0.245 - 2845 0.455 - 2878 0.317 - 2902 0.158 - 2945 0.602 Yes 2970 0.734 Yes 3031 0.149 - 3079 0.142 - 3103 0.440 - 3121 0.135 - 3154 0.218 - 3192 0.471 - 3209 0.271 - 3275 0.494 - 3289 0.164 - 3304 0.050 - 3322 0.039 -

Explain the output -- Processing locus 19: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATTTTTAAAATCTGCATTATCATAGGCACATACATGCCGAATATATAAAATAATTCGTGAAATAAGTTAAAGCTAGAA AATATGTAAGAAACTCAATGACGTGGCAAAAAAGAAGAGTATCCACCAGAAGTTGAGTCTAGGGGTATAAACGTAATTAA CATCACCTTATCCCCAACGTCTCAATTCTCTCTCTCGTGCTCTTCACTCGACGACACACGAAAACCGGAAATAAAAGTTT TCGGGTTTTCTTCAGCCGGACTGTTTTTCGCTCCCGCCGGAAATTCTCCCGGCTCGCCGTCGACGATGATGGTGTCTCCA CTCTGCGACTCTCAGTTACTTTACCACCGCCCCTCGATCTCACCTACCGCTTCTCAGTTCGTGATCGCGGATGGAATCAT CCTCCGGCAAAATCGTCTTCTGAGCTCTTCGTCGTTTTGGGGCACCAAATTCGGAAACACCGTCAAGTTGGGAGTATCTG GATGTAGTAGCTGCTCTCGGAAGAGAAGCACGAGTGTGAATGCTTCACTAGGTGGTCTTCTTAGCGGAATTTTCAAGGGT TCTGATAACGGAGAGTCGACTAGGCAACAGTACGCATCCATCGTCGCATCCGTTAATCGCTTGGAGACTGAGATTTCGGC TCTTTCGGATTCTGAGTTGCGAGAGAGGACTGATGCGTTGAAGCAACGTGCTCAGAAAGGAGAATCCATGGATTCACTTT TACCTGAAGCATTTGCTGTTGTGAGAGAAGCTTCCAAGAGAGTTCTTGGACTCAGACCTTTCGATGTGCAATTAATTGGT GGTATGGTTCTTCATAAAGGAGAAATAGCTGAAATGAGAACTGGTGAAGGGAAAACGCTTGTTGCTATTTTACCAGCTTA TTTGAATGCATTAAGTGGGAAAGGTGTTCATGTGGTTACAGTTAATGATTATCTTGCTCGAAGAGATTGTGAATGGGTTG GTCAAGTTCCTCGGTTCCTTGGATTGAAGGTTGGTCTAATCCAACAGAATATGACACCTGAACAAAGAAAGGAAAATTAT TTATGCGATATCACATATGTCACCAACAGTGAGCTTGGATTTGATTATCTGAGAGACAATCTAGCCACGAGTGTTGAGGA GCTCGTCTTGAGGGATTTCAATTATTGTGTGATTGATGAAGTTGATTCCATACTTATTGATGAAGCAAGGACTCCTCTCA TTATCTCTGGGCCTGCAGAGAAACCTAGTGACCAATATTACAAAGCTGCAAAGATTGCTTCAGCCTTTGAGCGGGATATA CATTACACTGTTGATGAAAAGCAGAAGACTGTTTTACTGACGGAACAGGGTTATGAGGATGCAGAAGAAATCCTGGACGT GAAAGATTTGTATGATCCCCGTGAACAGTGGGCATCATATGTTCTTAATGCCATTAAGGCAAAAGAACTTTTTCTCAGAG ATGTGAACTATATCATCCGAGCAAAGGAGGTTCTTATCGTGGATGAGTTTACTGGTCGTGTAATGCAGGGAAGACGTTGG AGTGATGGACTACATCAAGCTGTTGAAGCAAAAGAAGGCTTGCCTATTCAGAATGAATCTATTACTCTGGCGTCAATTAG TTATCAAAACTTCTTTCTGCAGTTTCCGAAACTTTGCGGGATGACGGGTACAGCATCGACCGAGAGTGCAGAATTTGAAA GCATATACAAGCTTAAAGTTACAATTGTACCCACAAATAAGCCCATGATAAGAAAGGATGAGTCAGATGTGGTTTTCAAG GCAGTCAATGGCAAATGGCGGGCAGTAGTAGTGGAGATCTCTAGAATGCACAAGACAGGTAGGGCTGTGCTAGTTGGCAC AACCAGTGTCGAGCAGAGTGATGAACTATCGCAACTGTTGAGGGAAGCTGGAATAACTCATGAGGTCCTCAATGCCAAGC CAGAAAATGTGGAGAGGGAAGCTGAAATTGTAGCACAAAGTGGCCGTTTAGGGGCAGTAACAATTGCCACAAATATGGCA GGGCGTGGGACAGACATAATTCTTGGTGGAAACGCAGAGTTCATGGCACGTTTGAAGCTTCGTGAGATACTTATGCCCAG AGTGGTAAAGCCTACTGATGGTGTTTTTGTATCTGTGAAGAAGGCCCCTCCCAAGAGAACATGGAAGGTGAATGAGAAGT TATTTCCATGCAAACTGTCAAATGAGAAAGCAAAGCTAGCTGAAGAAGCTGTACAATCAGCTGTAGAGGCTTGGGGCCAG AAATCGTTAACTGAGCTTGAAGCAGAGGAACGTTTATCTTATTCTTGTGAAAAGGGTCCTGTCCAAGATGAAGTTATAGG TAAACTGAGGACTGCATTTCTGGCGATAGCGAAAGAATATAAGGGCTACACTGATGAAGAAAGGAAGAAGGTGGTGGAAG CTGGTGGACTTCACGTGGTGGGGACAGAGCGGCATGAATCACGTCGAATAGACAATCAGTTGCGTGGGCGAAGTGGCCGG CAAGGGGATCCTGGAAGTTCCCGATTCTTCCTTAGTCTTGAAGATAACATATTCCGCATTTTTGGTGGAGATCGGATTCA GGGTATGATGAGGGCATTCAGGGTGGAAGATTTACCGATCGAATCCAAGATGCTTACTAAAGCTCTAGATGAAGCTCAGA GAAAAGTTGAGAATTACTTCTTTGACATCAGAAAGCAATTATTCGAATTTGACGAGGTTCTCAATAGCCAAAGAGATCGT GTTTATACAGAGAGAAGGCGTGCTCTTGTGTCGGACAGCCTTGAGCCTCTGATTATCGAGTATGCTGAATTGACAATGGA TGACATTCTAGAGGCAAATATTGGCCCAGATACTCCAAAGGAAAGCTGGGATTTTGAAAAGCTCATTGCGAAAGTTCAGC AGTACTGTTACCTGTTGAACGATCTCACTCCCGATTTGCTGAAAAGCGAAGGATCAAGTTATGAAGGGTTGCAAGATTAT CTCCGTGCCCGTGGCCGCGATGCATACTTACAGAAAAGAGAAATCGTGGAGAAACAATCACCAGGGCTAATGAAAGATGC CGAACGATTCTTAATCTTGAGCAATATTGATAGGTTATGGAAAGAACACCTTCAAGCACTCAAGTTCGTGCAACAAGCTG TGGGGCTCAGAGGATATGCGCAACGCGATCCACTCATCGAGTATAAGCTCGAAGGATACAATCTATTTCTGGAAATGATG GCTCAAATACGAAGAAATGTGATATACTCCATATATCAGTTTCAACCAGTGCGGGTAAAGAAGGACGAAGAGAAGAAGTC TCAGAACGGGAAACCGAGCAAACAAGTAGATAATGCTAGTGAGAAGCCTAAACAAGTTGGTGTCACAGATGAGCCATCCT CAATTGCAAGCGCCTAAGAGCGGTAAACGGTAAAATCTTTTTGGGTACAAGAGCGGGAATTCGTTTTTGGGAAAAAAGCT CGGTGAATACATTCGAGAAAGCTGGAATCACTAAAT ...................................i............................................ ...N.............N.............................................................. ................................................................................ .................................................................i..i........... ......................................................................i......... ................................................................................ ................................................................................ ................................N..................................N............ ...............................................................N................ ...i.............................i.............................................. .....N.......................N..............N...........................i....... ..................................................i............................. ..N.............N............................................................... ...................................N.......................N.................... ................................................................................ .............N......................................N.....N..................... ...........N..........................N........N................................ N.........................................N...................i................. ....N...............................................N........................... ........................................i....................................... ............................................N............N........N............. .......N......i..............................N.................................. ....................N......................................N...........i........ ......N...................................................................i..... ..........................................i.............................N....... .................N..........................................i..........N........ .......N.............N.......................................................... ...................................................................N............ .....................................................N.......................... .................................N.............................................. ................................................................................ ....i..i.........................................N..................N........... ................................................................................ .............................................................N.............i...N ................................................................................ ............................................................i................... ...................N.................................................i......i... ....................................i........................................... ...............N..........................................................i..i.. ................i............................................................... ................................N...................................N........... ................................................................................ .................................... Pos Score Pred ------------------------ 36 0.530 Yes 84 0.418 - 98 0.491 - 306 0.508 Yes 309 0.553 Yes 391 0.651 Yes 482 0.275 - 520 0.201 - 673 0.237 - 708 0.323 - 784 0.122 - 804 0.571 Yes 834 0.611 Yes 886 0.211 - 910 0.411 - 925 0.304 - 953 0.664 Yes 1011 0.538 Yes 1043 0.266 - 1057 0.276 - 1156 0.268 - 1180 0.186 - 1294 0.174 - 1333 0.188 - 1339 0.204 - 1372 0.216 - 1399 0.080 - 1408 0.094 - 1441 0.259 - 1483 0.097 - 1503 0.543 Yes 1525 0.139 - 1573 0.110 - 1641 0.561 Yes 1725 0.308 - 1738 0.445 - 1747 0.297 - 1768 0.281 - 1775 0.838 Yes 1806 0.414 - 1861 0.216 - 1900 0.311 - 1912 0.547 Yes 1927 0.389 - 1995 0.829 Yes 2043 0.585 Yes 2073 0.392 - 2098 0.327 - 2141 0.784 Yes 2152 0.185 - 2168 0.199 - 2182 0.410 - 2308 0.111 - 2374 0.386 - 2434 0.263 - 2565 0.714 Yes 2568 0.643 Yes 2610 0.446 - 2629 0.187 - 2782 0.175 - 2796 0.602 Yes 2800 0.340 - 2941 0.790 Yes 2980 0.189 - 3030 0.555 Yes 3037 0.575 Yes 3077 0.524 Yes 3136 0.145 - 3195 0.599 Yes 3198 0.602 Yes 3217 0.601 Yes 3313 0.301 - 3349 0.419 -

Explain the output -- Processing locus 20: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACGTTCTTGCACGTCGTCTCCAACTAGTATCGTGTAGACATGCTTCCACGACTTAGTTAGATGGAGTCCAAAAAAATAT GGAAAGTATAGCTGGATTTAGAGAAAATCTTTGCTATATAAACCTCAGCACTCATTCTTTAAAATACAAACCAAAACAAG AAATACTTTGCCAATGATATCTTCTTCTAAAATGGGGCCTTCTGCCCATCTCGTCTACGCTATAGGAGTTATCATCATGG CAACAATGGTTGCGGCATATGAGCCATACACAGATAGCTCACCTCCACCATACTCTGTGCCACTTCCCAAAGTGGAATAC AAATCTCCTCCATTACCAGATGTTTACAGTTCTCCACCACCACCACTAGAATACTCTCCAGCGCCTAAAGTTGATTATAA ATCTCCACCACCACCATACTATTCACCATCTCCTAAAGTTGAGTACAAGTCTCCTCCACCACCATATGTCTACAACTCTC CACCACCACCATATTACTCTCCATCCCCAAAGGTAGACTACAAGTCTCCTCCACCACCATATGTTTACAGTTCGCCACCA CCACCAATCTATTCACCATCTCCTAAGGTAGACTACAAATCTCCTCCACCACCATATGTTTACAGTTCCCCACCACCACC ATACTATTCGCCATCCCCTAAGGTGGAGTACAAATCTCCACCATCACCGTATGTTTACAACTCTCCACCACCATCATATT ATTCACCATCCCCTAAGGTAGATTACAAATCTCCTCCACCACCATATGTTTATAGTTCTCCACCACCACCATATTACTCT CCATCCCCTAAGGTTGTATACAAGTCTCCTCCTCCACCATATGTTTACAGTTCTCCACCACCACCATACTACTCACCATC ACCTAAGGTAGACTACAAGTCTCCACCACCACCATACGTCTACAGCTCACCACCACCACCATACTACTCACCTTCTCCTA AGATAGTCTACAAATCTCCCCCACCACCGTACGTCTACAGCTCCCCACCACCCCCATACTACTCACCTTCCCCTAAGGTA GACTACAAATCTCCCCCACCACCGTATGTCTACAGTTCTCCACCACCACCATACTACTCTCCATCCCCTAAGGTTGTATA CAAGTCTCCTCCTCCACCATATGTTTACAGTTCCCCACCACCACCATACTACTCACCAACCCCGAAGGTAGACTACAAAT CTCCTCCTCCACCATATGTTTACAGTTCTCCACCACCACCATACTACTCACCATCACCTAAGGTAGACTACAAGTCTCCA CCACCACCATACGTCTACAGCTCACCACCACCACCATACTACTCACCTTCTCCTAAGATAGTCTACAAATCTCCCCCACC ACCGTACGTCTACAGCTCCCCACCACCCCCATACTACACACCTTCACCAAAGGTTGTATACAAGTCTCCTCCTCCACCAT ATGTTTACAGTTCCCCACCACCACCATACTACTCACCATCCCCTAAGGTAGACTACAAATCTCCTCCTCCACCATATGTT TACAGTTCTCCTCCACCACCATACTACTCGCCATCTCCAAAGGTTGTATACAAATCTCCTCCTCCACCATATGTTTACAG TTCTCCTCCACCACCATACTATTCACCATCCCCTAAGGTAGACTACAAGTCTCCTCCACCACCATATGTTTACAGTTCTC CACCACCACCGTACTACTCTCCTTCACCAAAGGTTTTATACAAGTCTCCCCCACCACCGTATGTCTACAGTTCTCCACCA CCACCATACTACTCTCCTTCTCCTAAGGTAGTCTACAAGTCTCCCCCACCACCGTATGTTTACAGTTCTCCACCACCTCC ATACTACTCTCCTTCTCCTAAGGTAGTCTACAAGTCTCCCCCACCACCGTATGTCTACAGTTCTCCACCACCACCATACT ACTCTCCTTCTCCTAAGGTAGTCTACAAGTCTCCCCCACCACCATACGTTTACAGTTCTCCACCACCACCATACTACTCC CCTTCTCCTAAAGTATACTACAAGTCTCCTCCATCACCATACCACGCACCATCCCCTAAGGTTCTATACAAATCTCCACC ACACCCTCATGTATGTGTCTGTCCACCACCTCCTCCATGTTATTCTCCATCTCCAAAGGTTGTGTACAAGTCCTCCCCAC CACCGTACGTTTACAGTTCTCCACCACCACCATATCACTCTCCTTCTCCTAAGGTACACTACAAGTCTCCCCCACCACCG TATGTCTACAGTTCTCCACCACCACCATATTACTCTCCTTCCCCTAAGGTACACTACAAGTCTCCCCCACCACCGTATGT CTATAGTTCTCCACCACCACCATACTACTCTCCTTCTCCTAAGGTAGTCTACAAGTCTCCCCCACCACCGTATGTTTACA GTTCTCCACCACCTCCATACTATTCTCCTTCTCCTAAGGTAGTCTACAAGTCTCCCCCACCACCGTATGTCTACAGTTCT CCACCACCACCATACTACTCTCCTTCTCCTAAGGTAGTCTACAAGTCTCCCCCACCACCATACGTTTACAGTTCTCCACC ACCACCATATTATTCTCCTTCTCCTAAGGTAGTCTACAAGTCTCCTCCACCACCGTATGTCTACAGTTCTCCACCACCAC CATACTACTCTCCTTCTCCTAAGGTAGTCTACAAGTCTCCCCCACCACCGTACGTCTACAGCTCACCACCACCACCATAC TACTCTCCTTCTCCTAAAGTAGTTTACAAGTCTCCCCCACCACCGTACGTCTACAGCTCACCACCACCACCATACTACTC ACCTTCCCCTAAGGTAGACTACAAATCTCCCCCACCACCGTACGTCTACAGCTCACCACCACCACCATACTACTCACCTT CCCCTAAAGTAGACTACAAATCTCCTCCACCACCGTACGTCTATAGCTCACCACCACCACCATACTACTCACCTTCCCCG AAGGTAGACTACAAATCTCCCCCACCACCGTATGTCTACAGCTCACCCCCACCACCATACTACTCACCTGCCCCTAAGGT AGATTACAAATCTCCACCACCACCATACGTCTATAGTTCACCACCACCACCATACTACTCACCTTCCCCTAAGGTTGACT ACAAATCTCCTCCACCACCATACTACTCACCTTCCCCTAAGGTTGACTACAAATCTCCTCCACCACCATATGTCTACAGT TCTCCACCACCACCATCATACTCACCTTCTCCAAAAACTGAGTACTAAGCTTCACATTCACCTTCGTATTACTTGAAATT GTAATGAGATTCTTATATAAGCTACCAAGTTTTATTTCATTTCATCTTTGCTTTTGTTCTTCATGAT ........................................N....................N................N. ................................................................................ .............i.................i............................................i... .....N............N............................................................. ...................N............................................................ .................................................................i.............. ............................................................N................... .......................................................N........................ ..................................................N............................. .............................................N.................................. ........................................N....................................... ................................................................................ ................................................................................ .........................N...................................................... ....................N........................................................... ...............N................................................................ ................................................................................ ................................................................................ N..........................................................................N.... ......................................................................N......... .................................................................N.............. ............................................................N................... .......................................................N........................ ..................................................N............................. ................................................................................ ................................................................................ ........N...i.......................N........................................... ................................................................................ .......................................................................N........ ..................................................................N............. ................................................................................ ........................................................N....................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ...............................N................................................ ................................................................................ .....................................................................N.......... ................................................................................ ...N..........................................................N.... Pos Score Pred ------------------------ 41 0.278 - 62 0.383 - 79 0.394 - 174 0.760 Yes 192 0.865 Yes 237 0.664 Yes 246 0.423 - 259 0.192 - 340 0.285 - 466 0.523 Yes 541 0.310 - 616 0.384 - 691 0.361 - 766 0.254 - 841 0.377 - 1066 0.347 - 1141 0.333 - 1216 0.351 - 1441 0.275 - 1516 0.240 - 1591 0.304 - 1666 0.358 - 1741 0.378 - 1816 0.336 - 1891 0.457 - 2089 0.444 - 2093 0.632 Yes 2117 0.194 - 2242 0.397 - 2317 0.389 - 2392 0.436 - 2467 0.454 - 2617 0.477 - 2992 0.433 - 3190 0.417 - 3284 0.063 - 3343 0.048 -

Explain the output -- Processing locus 21: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGAATTTCAGTGTGATTAAATTAACAAATCTCTTTGCTTGAAAAGTCCCTTTTTCTTCGTCTTCAGTTAGCAGTTTAA TTGGAAGTAAAATTAGCTTGATTTTGCATGTTTTCAGCTGCGTTGGAGACTATGGAAGAAGTTCAGCCTCCAGTGACCCC GCCCATTGAACCAAATGGGAAAAGAAGCGAAGCCTCTCTCTTGGACATATGCGAGAAAGTTCTGTCTCTTGATGGGAGCA CTTGCGATGAAGCTTTGAAGTTGTTTACAGAAACCAAACGAATTTTGTCAGCAAGCATGTCTAACATTGGAAGTGGAACG CGGGAAGAAGTAGAGAGGTTCTGGTTTGCGTTTATTCTCTATTCAGTGAAGAGGCTTAGTGTGAGAAAAGAAGCGGATGG TCTGTCAGTGTCTGGTGATAATGAGTTTAATCTATGTCAGATACTGAGGGCTCTGAAGCTAAATATTGTGGATTTTTTTA AAGAGTTACCTCAGTTTGTGGTCAAGGCTGGATCTGTACTGGGTGAACTTTACGGCGCAGACTGGGAGAACAGACTTCAG GCAAAGGAGGTGCAGGCTAACTTTGTGCATCTTAGCCTTCTAAGCAAATACTACAAACGTGGGTTCCGGGAATTCTTTTT GACATATGATGCAAACGCAGAAAAGAACTCAGCAAACTCTTCTACCTATTTGCTGGATAGTTATCGTTTTGGATGGCTAC TCTTTTTGGCACTCCGAAACCATGCGTTTAGTCGATTTAAGGACCTCGTGACATGCTCAAATGGCGTAGTTTCTATATTG GCTATTTTGATCATACATGTTCCTTGTCGGTTTAGAAATTTCAGCATCCAAGATTCTTCTCGCTTTGTTAAGAAAGGTGA CAAAGGTGTAGACTTGGTTGCATCACTTTGCAAGATATATGACGCCTCAGAAGATGAGTTGAGGATAGTAATTGACAAGG CAAATAATTTGGTAGAAACCATACTGAAGAAAAAGCCATCTCCAGCATCTGAGTGCCAAACTGACAAGCTAGATAATATT GACCCAGATGGCTTGACCTACTTTGAGGATTTACTGGAAGAGACGTCCATCTCAACTAGCTTAATTACACTTGAAAAGGA TTACTATGATGGTAAAGGCGAACTTGATGAGAGGGTATTCATCAATGAAGAGGATAGCTTACTTGGATCTGGAAGCTTAT CTGCAGGAGCTGTTAATATTACTGGTGTTAAGAGGAAAATTGATGCTTTGAGCTCACCTGCAAGGACATTTATAAGCCCA CTTTCTCCTCATAAGTCGCCTGCTGCTAAGACAAATGGTATTAGCGGTGCTACCAAGTTGGCAGCAACACCAGTGAGCAC AGCAATGACAACTGCCAAGTGGCTCAGGACTGTCATATCCCCGCTTCTGCCAAAACCTTCTCCTGGGTTGGAACATTTCC TTAAATCATGTGATAGGGATATAACAAATGACGTCACACGAAGAGCACACATAATATTGGAAGCTATTTTCCCAAATAGT TCCCTTGGTGCCCAATGTGGAGGTGGAAGTTTGCAAGCTGTTGACCTGATGGATGACATATGGGCAGAGCAGCGCAGATT AGAAGCTTGTAAGTTATACTACAGAGTTCTTGAGGCAATGTGTAAAGCAGAAGCTCAGATTTTGCATGCAAATAATCTGA ACTCTTTATTGACAAATGAGAGGTTCCATAGATGCATGCTTGCTTGCTCAGCTGAATTGGTACTGGCTACCCACAAAACA ATTACAATGTTGTTCCCAGCTGTTCTGGAGAGGACTGGGATCACAGCCTTTGATCTCAGCAAGGTAATTGAGAGTTTCAT ACGACATGAAGATTCTCTGCCTAGAGAGTTGAGACGACATCTGAATTCACTGGAGGAACGGCTTCTAGAGAGTATGGTAT GGGAGAAAGGCTCTTCAATGTACAATTCTCTGATTGTTGCCAGGCCATCGCTTGCATTGGAGATAAATCAGCTCGGTTTA CTAGCTGAACCAATGCCATCTCTGGATGCAATCGCAGCACTTATTAATTTCTCTGACGGAGCAAATCATGCATCATCTGT ACAAAAGCATGAAACTTGTCCAGGACAAAATGGGGGGATTAGATCGCCCAAAAGATTATGTACTGATTACCGCAGCATTC TAGTTGAACGCAATTCCTTTACATCACCAGTAAAGGATCGTCTGTTGGCCTTAGGCAACGTTAAATCCAAGATGCTGCCA CCTCCGTTGCAGTCTGCATTTGCCAGCCCAACACGGCCCAACCCAGGAGGTGGAGGAGAAACTTGTGCAGAAACTGGAAT CAATATTTTCTTCACAAAGATTAATAAATTGGCTGCTGTAAGAATCAATGGAATGGTGGAAAGACTACAACTTTCACAGC AAATAAGGGAGAGTGTGTATTGTTTCTTCCAACATGTACTTGCTCAGCGGACTTCTCTTTTATTCAGTCGACACATTGAC CAGATCATTCTCTGTTGCTTCTACGGAGTGGCCAAGATATCCCAAATGAGCCTGACTTTCAGGGAAATCATATACAACTA CCGGAAGCAACCACAGTGTAAACCATTAGTTTTCCGCAGCGTTTATGTGGATGCGTTACAATGTCGCCGTCAAGGGAGAA TAGGGCCAGATCATGTTGACATCATCACATTCTACAATGAAATATTTATTCCTGCCGTAAAGCCGCTGCTGGTGGAGCTA GGTCCTGTAAGAAACGACCGGGCTGTGGAAGCCAATAATAAGCCTGAAGGTCAATGTCCCGGATCGCCAAAGGTGTCTGT GTTTCCAAGTGTTCCAGACATGTCCCCTAAAAAAGTATCTGCAGTGCACAATGTTTATGTTTCTCCTCTTCGGGGATCAA AGATGGATGCTCTTATTTCACACAGTACAAAGAGTTACTATGCTTGTGTTGGAGAGAGTACACATGCTTACCAGAGCCCT TCAAAGGACCTATCTGCCATCAACAACCGCTTGAACAACAGCAGCAGCAACCGCAAGAGGACGCTAAACTTTGACGCAGA AGCAGGGATGGTCAGCGATTCCATGGTAGCAAATAGCCTTAACCTCCAAAACCAAAATCAAAACCAAAATGGAAGCGATG CATCGTCCTCAGGTGGTGCCGCACCCCTTAAAACCGAGCCAACAGATTCATAGATATCTCTCTCTACTTGCTACACCAAC TTCTCTTCAGTTATAGCATCTGTAAATCCTTATGTTGCAGAGTTTGCTTTTATGTTTAGCTTTCTAGTTTAT ................................................................................ ...........................N.......................i............................ ..............N.................................N......................N........ ......N.................................................i....................... ............................................................................N... ....................N............N.............................................. ................................................................................ ................................................................................ .....N..N...............................................................i....... .....................N..............................N.......N................... ................N............................................................... ......................................N..............N.......................... ................................................................................ .......i........................................................................ .....N..N.................N.................N................................... ..........................................N..................................... ..................................N............................................. ....i........................................................................... .......i...................i.................................................... ..............N.................................N...N......i.................... .....................................N...........................N.............. ...............N...............i...i............................................ ......i......................................................................... .....N...................................................................N....i. .................i.............................................................. ............i............N.........................................i............ ........N....................i...........................N...................... .......................................................................i........ ................................................................................ ...............................................N....N........................... .................................i.............................................. .............................................N.................................. ............................................N.....N.........i................... ............N.......................N........................................... .....................................................N.......................... ...................N..............................N.....N....................... ..i...N................................i.......................i................ ................................................................................ .......i..............i.............................................i........N.. ................................................................................ ...............................N...................N.................... Pos Score Pred ------------------------ 108 0.329 - 132 0.582 Yes 175 0.331 - 209 0.269 - 232 0.428 - 247 0.268 - 297 0.645 Yes 397 0.169 - 421 0.169 - 434 0.400 - 646 0.324 - 649 0.183 - 713 0.635 Yes 742 0.320 - 773 0.109 - 781 0.362 - 817 0.174 - 919 0.124 - 934 0.268 - 1048 0.579 Yes 1126 0.345 - 1129 0.235 - 1147 0.195 - 1165 0.166 - 1243 0.153 - 1315 0.411 - 1365 0.510 Yes 1448 0.510 Yes 1468 0.607 Yes 1535 0.279 - 1569 0.459 - 1573 0.286 - 1580 0.584 Yes 1638 0.280 - 1666 0.230 - 1696 0.450 - 1712 0.595 Yes 1716 0.534 Yes 1767 0.677 Yes 1846 0.196 - 1914 0.347 - 1919 0.589 Yes 1938 0.611 Yes 2013 0.728 Yes 2026 0.498 - 2068 0.508 Yes 2089 0.473 - 2110 0.692 Yes 2138 0.437 - 2232 0.587 Yes 2368 0.271 - 2373 0.463 - 2434 0.580 Yes 2526 0.276 - 2605 0.241 - 2611 0.207 - 2621 0.669 Yes 2653 0.262 - 2677 0.252 - 2774 0.424 - 2820 0.447 - 2851 0.291 - 2857 0.200 - 2883 0.857 Yes 2887 0.193 - 2920 0.533 Yes 2944 0.619 Yes 3048 0.713 Yes 3063 0.718 Yes 3109 0.645 Yes 3118 0.487 - 3232 0.046 - 3252 0.049 -

Explain the output -- Processing locus 22: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTTGATCTAGTAATTTCACAAAAAGCGCATATCTTCCGGCTAATGATATTAGATTTACTCATATGTGTAATGTGTATGT ATGGTTGAAAGTTGAAGCTAAATTAGGTTTAAGTCACTCATATCATATGTGTGTGTTATCTTGTATAGTGTAATACAAAA TTCAATACAAAATTTTCCAATAAGCAAAAATAGTCGTCAGTGCGAGTTCAACTTTAAACTGTTTCAAACTTTATATTCAT TACGAATGCAACAGTCCCTCTCTTGTTTCTCCACGCTTCGCTTTCTCCTTCCATTTTCTTCCAAACTCTCTCCGCTTCGT CCTCTCTTTCACACCGCCGCTATGCCTCCTCACGGTCCTAATTCTCAAGGTGGTCGTCGAGGCGGAGGTCATTCCTCCGG TCGTCGTGGTGGACGCGGCGGCGGCGGTCGTGGCGGTGGTGGTGGCGGCCGGGGGGAACAGCGATGGTGGGATCCTGTGT GGAGAGCTGAGCGTTTGCGCCAGCAACAAGCTGAGATGGAAGTACTTGATGAGAATGAATGGTGGAACAAGATTGAGCAA TGGAAAACAGGAGGAGAACAAGAGATGCTAATTAAGCGTAATTTTAGCCGTGGAGATCAGCAAACACTTTCTGATATGGC TTTGCAGATGGGTCTTTACTTTCATGCGTACAATAAAGGGAAGGCTCTTGTTGTTAGCAAAGTTCCTTTACCAGATTATC GAGCGGATCTTGATGAACGGCATGGATCCACTCAGAAAGAGATTAAAATGTCCACAGAGACTGAGAGAAAACTTGGAAGT CTTTTGAAAACAACACAAGAATCAGGGTCCTCTGGTGCCTCTGCTTCAGCTTTTAACGATCAACAGGATCGTACTTCAAC TCTTGGCTTGAAAAGACCTGATTCTGCTTCCAAATTGCCGGATTCTCTTGAGAAAGAAAAATTCAGTTTTGCACTCAAGG AAAGGCAGGAAAAGCTTAAGGCAACTGAGAGTGTAAAAGCACTTAAAGCGTTCAGAGAAAAGCTACCTGCTTTTAAAATG AAAGAGGAGTTTCTTAACTCTGTTTCACAAAACCAGGTATTGGTAGTTTCAGGAGAGACCGGGTGTGGTAAAACAACACA GCTTCCTCAATTTATATTGGAAGAAGAAATATCATCCCTAAGAGGTGCTGATTGCAACATAATTTGCACTCAACCTCGAC GTATATCGGCCATATCTGTTGCTTCTCGTATATCTGCTGAAAGAGGTGAATCCATTGGTGAATCTGTGGGTTATCAGATT CGTCTAGAATCAAAGCGTTCTGATCAAACACGATTGCTATTTTGCACTACTGGTGTTTTACTCCGGCGGCTGATTGAGGA TCCTAATCTAACTAACGTAAGCCATTTGCTCGTGGATGAGATTCACGAAAGAGGCATGAATGAAGATTTTCTACTAATCA TACTACGCGATCTCCTTCCTCGACGCCCAGATTTGCGTCTTATTCTGATGAGTGCTACGATTAACGCTGACATGTTCTCA ACATACTTTGGGAACTCTCCCACTATGCACATCCCGGGTTTCACATTTCCTGTGGCAGAGTTATTCCTAGAAGATGTATT GGAGAAAAGTCGTTATAATATTAAGTCGTCTGACTCGGGGAATTATCAAGGTAGTTCGAGAGGTAGAAGAAGAGAATCAG AATCCAAGAAAGATGATCTGACTACTCTTTTTGAGGATATTGACATCAATTCTCACTATAAAAGTTATAGCTCGGCCACA AGAAATTCTCTTGAAGCATGGTCTGGTGCACAGATTGATGTGGATCTGGTGGAGGCAACAATTGAACATATTTGTCGTCT TGAAGGTGGTGGGGCGATTCTTGTCTTCCTTACTGGCTGGGATGAGATTTCAAAACTGCTTGAAAAAATTAACATGAACA ACTTTCTTGGAGATTCGAGCAAGTTCTTAGTTCTTCCTTTACATGGTTCAATGCCGACGGTTAATCAACGTGAAATTTTT GACCGTCCACCTCCTAACAAACGGAAAATAGTTTTGGCTACAAACATTGCCGAGAGTAGTATTACAATTGATGATGTTGT ATATGTTGTGGATTGTGGTAAAGCAAAGGAAACTAGCTATGATGCTTTGAACAAGGTGGCATGTCTCTTGCCATCATGGA TATCAAAGGCTTCAGCTCACCAGAGACGAGGTCGTGCTGGACGTGTTCAAGCTGGAGTTTGTTATAGGTTGTATCCAAAA GTTATCTATGATGCTTTTCCACAGTATCAGTTACCAGAAATCATACGGACTCCATTACAAGAGCTATGCCTGCATATCAA AAGTTTGCAAGTTGGATCTATAGGCTCATTCTTGGCAAAGGCACTGCAGCCACCAGATGCACTTGCTGTTGAGAATGCTA TTGAACTTCTCAAAACAATTGGGGCTTTAAATGACGTGGAAGAGCTTACACCCCTTGGTCGGCATCTTTGCACTCTACCC GTGGACCCAAATATTGGAAAGATGCTTCTGATTGGAGCTATCTTCCAGTGCGTCAATCCTGCTTTGACAATCGCTGCTGC TCTGGCGTATCGTAGCCCATTTGTCTTACCATTAAATAGGAAAGAGGAAGCTGATGAAGCCAAACGATATTTTGCTGGTG ATTCCTGCAGTGATCACATTGCTCTTCTTAAGGCATATGAGGGATATCGGGATGCAAAGCGTGGTGGAAACGAAAAAGAT TTTTGTTGGCAAAACTTTCTTTCCCCTGTAACTCTAAGGATGATGGAGGATATGAGAAACCAGTTTCTTGATCTTCTATC AGATATAGGGTTTGTAGACAAATCAAAGCCAAATGCATACAACCAATATAGCTACGATATGGAGATGATAAGTGCGGTTC TATGTGCGGGGCTGTACCCAAATGTTGTCCAATGCAAAAGAAGAGGGAAGCGCACTGCGTTCTATACTAAAGAGCTGGGT AAAGTTGATATCCACCCTGGATCTGTCAACGCAAGAGTGAATCTCTTCTCTTTGCCGTACTTGGTCTACAGCGAAAAGGT GAAGACGACAAGTGTTTATATCCGAGACTCGACAAATATATCAGACTATGCACTCCTTATGTTTGGTGGCAATCTTATCC CAAGCAAAACCGGGGAAGGTATAGAGATGTTGGGAGGGTATCTCCATTTCTCAGCCTCGAAGAACATCTTAGAACTGATA CAGAGATTGAGGGGAGAGGTGGACAAGCTACTGAATAAGAAGATAGAGGATCCGAGTTTGGACATAACAGTGGAGGGGAA AGGAGTGGTCTCAGCTGTGGTGGAGTTGTTACGTAGCCAAAAACATTAGGTATTAAGCAAAATTCCAGTGAGTCTTATCG TAGTGTAGAGGGATTAAGAAACGTTAGTTATTGGAAAACGAGGTGAATAGACTTTAAACTCTGCTTAA ...........................................N...................N......N.....N... N.............................................i................................. ................................................................................ .....i.......................................................................... .....................i.......................................................... ...............................................................i................ ...................................N............N.....i...i....................N ........................i..................................................i.... .......i...............N........................................................ ............N........N.........................i................................ ................................................................................ ................................................................................ .............................................................................i.. ................................................................................ ................................................................................ ................................................................................ ................................................................................ ...................................N...................i...N.................... ...............................................N.......................i........ ........................i................................................i...... ................................................................................ ............N................................................................... .................i...................N.......................................... .........................................N...............................N...... ..........................................N.......N............................. ......................................................................N..N...... ..N...................................N..N..................N..............N.... ................................................................................ .......N..N......................................................i.............. ........................................................N.................N..... ..............................N................................................. .....................i.......................................................... .....................................................i.......................... ....................................N..............N............................ .......................................i..N........N............................ ................................i.........................i.....N............... ................................................................................ ...............................................N..........N..................... ..........................N..................................................... ................................................................................ ................................................................................ .................................................................... Pos Score Pred ------------------------ 44 0.092 - 64 0.375 - 71 0.400 - 77 0.238 - 81 0.426 - 127 0.628 Yes 246 0.552 Yes 342 0.869 Yes 464 0.591 Yes 516 0.447 - 529 0.124 - 535 0.501 Yes 539 0.605 Yes 560 0.343 - 585 0.608 Yes 636 0.729 Yes 648 0.641 Yes 664 0.262 - 733 0.191 - 742 0.182 - 768 0.688 Yes 1038 0.500 Yes 1396 0.160 - 1416 0.513 Yes 1420 0.282 - 1488 0.327 - 1512 0.635 Yes 1545 0.583 Yes 1594 0.580 Yes 1693 0.309 - 1778 0.574 Yes 1798 0.204 - 1882 0.064 - 1914 0.407 - 1963 0.111 - 1971 0.355 - 2071 0.132 - 2074 0.270 - 2083 0.136 - 2119 0.228 - 2122 0.157 - 2141 0.169 - 2156 0.106 - 2248 0.114 - 2251 0.216 - 2306 0.547 Yes 2377 0.215 - 2395 0.138 - 2431 0.318 - 2502 0.690 Yes 2614 0.548 Yes 2677 0.210 - 2692 0.115 - 2760 0.772 Yes 2763 0.487 - 2772 0.437 - 2833 0.550 Yes 2859 0.536 Yes 2865 0.444 - 2882 0.546 Yes 2902 0.287 - 2912 0.157 - 3088 0.319 - 3099 0.321 - 3147 0.497 -

Explain the output -- Processing locus 23: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAAACGTTAATAATTGGTGCTAGGCTGGTTTAGTATCAATTAAACCATTAATCACTTTATACTTTATCCCCTCCAGCTC TCTCTTTCTCAAAACTTATAAGTCAAAAGTCAAAGTCAAAAGTCAATGGTGAATACGCCTTTTCCAATTTCTCCATCGTT TATGGCTCATCTACTGATCCACAGACATTTTTGTTCTCGCTACTCTATTCTCATGGCTTCCTCTTCTTCTTCGCCTCGCA CCTGGAGATACCGCGTCTTCACGAGCTTCCATGGACCTGACGTCCGTAAAACCTTTCTCAGTCATTTACGTAAACAGTTT ATCTGCAATGGGACTACCATGTTCGATGATCAAGCAATCGAAAGAGGCCAAACCATTTCCCCTGAACTCACACGAGGGAT TAGGGAATCGAGGATCTCGATCGTAGTGCTCTCGAAGAACTATGCTTCATCGAGCTGGTGTTTGGATGAGCTGTTGGAGA TTTTGAAATGCAAGGAAGATATTGGGCAAATAGTGATGACTGTCTTCTACGGAGTAGATCCATCTGATGTGCGCAAACAA ACCGGAGATATCTTGAAAGTTTTCAAGAAAACTTGTTCTGGTAAAACGGAGGAGAAGAGGCGAAGATGGAGCCAAGCTTT GAACGATGTTGGCAACATTGCCGGAGAACATTTCCTTAACTGGGATAATGAATCGAAGATGATGGAGAAGATTGCAAGAG ATATTTCAAACAAAGTAAACACTACAATCTCTAGGGATTTTGAAGACATGGTGGGAGTTGAGACACACCTGGAGAAAATA CAGTCTTTGTTACATTTAGATAATGATGATGAAGCTATGATTGTTGGAATCTATGGCCCTGCAGGTATTGGTAAGACTAC CATTGCCAGAGCTTTGCACAGTCTACTCTCTGATAGGTTTCAGCTTACTTGTTTTATGGAGAATCTTAGGGGAAGCTATA ATAGTAGTCTTGACGAGTATGGTTTGAAGCTGCAATTACAAGAACAACTTCTTTCCAAGATTTTAAACCAAACTGGTATG AGGGTATACAATTTAAGTGCAATACAGGGAATGCTATGCGACCAAAAAGTGCTTATCATTCTTGATGATGTGGACGATCT GAAGCAACTAGAAGCTTTGGCTAATGAAACTAAGTGGTTTGGTCCTGGGAGTAGGGTTGTAGTGACCACAGAAAATCAAG AGCTTTTGAAGCAACATGACGATATTAAGAATACATACTATGTGGATTTTCCTACTCAGAAAGAAGCTCGTCAGATCTTT TGTAGATATGGTTTTAAACAGAGCACTCCACAAGATGGTTTCGAAAATCTTTCCGAAAGAGTTATAAAGCTTTGCAGCAA ACTTCCACTAGGTCTTAGTGTCATGGGTTTATATCTACGAAAAAAGACAGAAGATGACTGGGAAGATATACTGCATAGGC TAGAAAGTAGCTTTGATTCAGTTGATCGAAATATCGAGAGAGTACTAAGAGTCGGATATGACGGTTTACATGAGAAGGAT CAATTGTTATTTCTCCTCATTGCATTCTTCTTCAACTACAAAGACGATGATCATGTGAAAGCAATGCTTGCTGACAATAA CTTGAATGTCAGACTCGGTTTGAAAACTCTCGAGTATAAATCTCTTATACAAAGATCGTCTGGAGGAAATATAGTGATGC ACAAGTTACTACAACAAGTGGGTAGAGAAGCGGTTCAAAGACAAGAGCCTTGGAAACGCCAAATCTTAATTGATGCTCAT GAGATTTGTAATGTCCTCGAAACTGATTCTGGTTGTGCAAATGTGATGGGCATATCCTTTAATGTATCTACAATCCCGAA TGGCGTGCATATAAGCGCAAAAGCTTTTCAAAATATGCGTAATCTTCGATTTCTAAGCATCTACGAGACAAGACGTGATG TAAACCTTAGAGTAAATGTACCCGATGACATGGATTTCCCACATCGTCTAAGGTCTTTACATTGGGAGGTATACCCAGGA AAGTCTCTTCCTTCTACATTTAGGCCAGAATATCTTGTGGAACTCAACTTGCAAAATAACAAGCTAGAGAAGCTTTGGGA AGGAACCCAGCCTCTTACAAATCTCAACAAGTTGGAATTGTGTGGGTCCTTGAGATTGAAGGAACTTCCAGATCTTTCAA GTGCTACAAATCTCAAGAGACTAGATCTAACTGGTTGTTGGAGTTTGGTAGAGATTCCTTCCTCTGTTGGAAATCTTCAC AAACTAGAAGAGTTGGAGATGAATTTATGTTTACAGCTACAAGTTGTTCCGACTCACTTCAACTTGGCATCTCTTAGATC ACTCAGGATGTTGGGATGCTGGGAATTAAGGAAATTTCCAGGTATTTCTACGAACATCACATCCCTCGTAATCGGAGATG CAATGTTAGAAGAAATGCTTGAATCAATTAGGCTTTGGTCTTGCCTTGAGACTCTGGTTGTATATGGCAGTGTCATTACA CATAATTTTTGGGCAGTAACTTTAATAGAGAAAATGGGCACAGATATAGAGAGGATTCCTGATTGCATCAAAGATCTCCC TGCGTTAAAATCGCTTTATATAGGTGGCTGTCCAAAACTTTTTTCACTGCCAGAGCTCCCTGGCTCACTCAGAAGACTAA CGGTAGAAACTTGTGAATCACTCAAGACTGTATCTTTTCCTATTGATTCTCCAATTGTGAGTTTCAGTTTCCCCAACTGC TTCGAATTGGGTGAAGAAGCACGGAGAGTGATTACACAGAAAGCAGGTCAGATGATAGCATACTTACCAGGAAGGGAAAT ACCTGCGGAGTTCGTGCACAGAGCTATAGGAGATTCGTTGACCATCCGTTCATCTTTCTGCTCCATATTTAGGATTTGCG TTGTGGTTTCCCCTAAATCAGAGATGAAAGAAGAATATGTTGGTTTTATGTGTCGCAAACGCATAAACGGTTGCCCCATT GGCGATAACTTATTTAAGGCACAGCTCCGTAAACTCCAAGCGGAACATCTATTTATATTTCAATTTGAATTCCTTGAGGA AGACGGATGGCTGGAACAGGACAACGAGGTATTGTTCAAATTCACGACCTCATCCGAGGAACTAGACATTATTGAATGTG GTATCCAGATATTGACAGGCGAAACCAACAGAAACATAAGCACCTATGGAAGCTATGAATCCAGATCAGAGCAAGTCTCT GAATACGAAGATGGATATCACTCTGATAGGAGACTTGAGTTCCACGAACAAAAGTCCTTGCCCGGGTGGGGTTTCTGTGG AATTTTTCATGGCTTCTTGAGATGTTTTATGGTCTGACAATGTGGCTGCCGTTCATGAAATTTCTCAGAAATGGCCTAGT CAAGTGGGACCATCTCTCTCTTCATCCTAGCCACGGTAGTCTTATCGTGGATGTTG ................................................................................ .............................................N.................................. ..............................i................................................. .......N..........i......N...................................................... .........................................N.......................N.............. .......i...........................i..............................N............. .................................................................N.............. .....N.........................................N..........i..i.................. ...............................................i................................ ......................N..N..N.......N...............N........................... .......................................................N........................ ..................N..........................................................N.. ..............................N....N............................N..N............ .......................N........................................................ ...............N.......................N........................................ .......N..........................i............................................. ......................N..............................i.......................... .........................................................N...........N.......... ..............................................N.....N..........i................ .....i......................................................................i... ........................................................................N.....N. ..........N.............................N....i...............N.................N ..................................i..........................................N.. ...............N........N....i.................................................. ................................................................................ ................................................................................ ................................................................................ ..................N.......i..................................................... .......N.......N.............................................................N.. ..N...........i................................................N................ .................................i.............................................. ................................................................................ ................................................................................ ...................................................N............................ ................................................................................ .......................i............N..........N................................ ................................................................................ ......i....................................................................N.... .............................................i........N......................... ..........N..................................................................... ........i............N......N..........N..............N...............N......... ..................................................N..... Pos Score Pred ------------------------ 126 0.459 - 162 0.442 - 213 0.607 Yes 271 0.513 Yes 328 0.439 - 339 0.621 Yes 346 0.351 - 442 0.299 - 466 0.352 - 488 0.515 Yes 516 0.854 Yes 547 0.255 - 626 0.265 - 646 0.241 - 688 0.199 - 699 0.733 Yes 702 0.825 Yes 768 0.591 Yes 823 0.192 - 826 0.319 - 829 0.172 - 837 0.379 - 853 0.237 - 936 0.474 - 979 0.335 - 1038 0.450 - 1071 0.445 - 1076 0.186 - 1105 0.168 - 1108 0.353 - 1144 0.083 - 1216 0.148 - 1240 0.463 - 1288 0.200 - 1315 0.508 Yes 1383 0.424 - 1414 0.647 Yes 1498 0.392 - 1510 0.219 - 1567 0.231 - 1573 0.390 - 1584 0.668 Yes 1606 0.586 Yes 1677 0.501 Yes 1753 0.189 - 1759 0.226 - 1771 0.392 - 1801 0.243 - 1806 0.714 Yes 1822 0.242 - 1840 0.366 - 1875 0.587 Yes 1918 0.487 - 1936 0.326 - 1945 0.210 - 1950 0.601 Yes 2259 0.141 - 2267 0.685 Yes 2328 0.293 - 2336 0.370 - 2398 0.221 - 2403 0.164 - 2415 0.554 Yes 2464 0.288 - 2514 0.639 Yes 2772 0.442 - 2904 0.633 Yes 2917 0.423 - 2928 0.296 - 3047 0.682 Yes 3116 0.216 - 3166 0.570 Yes 3175 0.397 - 3211 0.267 - 3289 0.677 Yes 3302 0.306 - 3309 0.234 - 3320 0.275 - 3335 0.107 - 3351 0.315 - 3411 0.034 -

Explain the output -- Processing locus 24: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGGGAAGATGTTCAATTTGAACTGTTATTAGTAAAACTGTCGATCTTTTTATTCTGTGCCACTATACACGAATGTTGCT GAATTACTAAAGTTTAGGTAGTTTTGTCAGTATGGTTTGCTTTTGCTTGTATGTCTTATGATGAGACATGATTCCAGTCT TTTTATCTCTGTCTTGTTCAGGAAAAAAATGAGTGATTACGGTGAAAAGACTTGCCCTTTGTGTGCTGAGGAGATGGATT TGACTGACCAGCAATTGAAGCCTTGCAAATGTGGCTATCAGATTTGTGTTTGGTGCTGGCACCATATAATGGACATGGCA GAGAAAGATCAATCAGAAGGGCGCTGTCCAGCTTGCCGCACTCCATATGACAAAGAAAAGATTGTTGGGATGACTGTTGA TCAGGAGAGACTGGCCTCTGAAGGCAACATGGATCGCAAAAAAATACAAAAGTCGAAACCGAAATCTTCTGATGGGAGAA AGCCACTAACAAGTGTCCGAGTGGTTCAAAGAAATCTTGTTTACATTGTTGGATTGCCCCTTAATCTAGCAGATGAAGAT CTTCTCCAGCGTAAAGAATATTTTGGTCAGTATGGAAAGGTTCTAAAGGTTTCGATGTCCCGAACAGCAACAGGTCTCAT CCAACAGTTTCCTAACAACACATGCAGTGTATACATCACATATGGCAAAGAGGAAGAGGCCATTCGATGTATTCAGTCTG TTCATGGGTTTATCTTGGATGGTAAAGCACTGAAGGCATGTTTTGGGACAACCAAGTATTGCCATGCATGGTTGAGAAAC GTGGCATGCAACAATCAGGATTGCCTCTATTTGCATGAGGTTGGCTCTCAAGAGGATAGTTTTACAAAAGATGAGATCAT ATCAGCCCATACAAGAGTTCAGCAAATCACTGGAGCAACAAATACTATGCAATACCGTTCAGGGAGCATGCTACCTCCAC CATTGGATGCTTATACCAGTGACAGTTCTACTGGAAATCCAATTGCAAAAGTTCCTTCTAGTACTTCAGTTAGTGCTCCT AAGAGTTCCCCACCTAGTGGGAGCTCGGGTAAATCAACTGCTCTTCCTGCTGCAGCATCATGGGGGGCACGTTTAACAAA TCAACACTCTTTAGCAACTTCAGCTTTGTCAAATGGATCTTTGGATAATCAAAGATCAACATCAGAGAATGGAACTTTGG CTACGTCTACAGTTGTTACAAAGGCAGCTAATGGGCCTGTATCCTCTAGTAACAGTCTTCAGAAAGCACCTCTCAAGGAA GAAATTCAATCATTGGCTGAAAAAAGCAAACCTGGTGTGTTGAAGCCTTTGCAGCAGAAGATTGTGTTGGATCCCGAGTC CAAGAGAACTACATCACCTAATAGAGACCCTAGTAGCAATCAGATATCCTGTTTAGTAGAGTCTTCCTACAATAGCAGAG TCATTGACAAGCCTTCTGCTGTTGAGAATTCTCTTGAACACACAAGTGAAATTGCTGAAGATGTCTTCGATGTTGGTAAA TTGTCTGCTGATGTTGCATGGATGGGGATAACTACAAATTCTAGGGATGAAACCCCTGGTGTTCCAGTGGTTATCGGTAC TCATTGTGATCTTGGTTCTATTACACAATCTGATAACGATGTACAAAATCTCGAGCAATGTAGAAAACAGTCTCCTACAA ACACTTATGCAGAAGCTGACATATCACTGAATGGAATTCATGGTTCAAGGCCTGAGTGGGATTGGAGATCAGGTTTGCAA TCCCAGATAGATGTTAAGGAACCGTTGGAAGTCAATGATTTCTCATCATTCAATAACAACAGACGTGGTATTGCAGAGGC TGTTAGCCACTCGACTTCTAAGTTTAGTTCATCAATCTCAATTCTGGATTCAAATCATCTAGCTTCTCGTTCGTTCCAGA ACCGGGAAACTTCTTGTGGGATGGATTCAAAAACTGGATCCTCCTTTGAAATAGGTAGTGATAGATTGCATCTTCCAAAT GGATTTTCTGAGAAAGCTATGTCCAATATGGAGCATTCATTATTTGCTAATGAAGGCAGAAGCAACATTCAGAATACAGA GGATGATATCATTTCAAACATTTTAGACTTTGATCCCTGGGATGAATCGTTGACTTCGCAACACAATTTTGCAAAATTAC TTGGTCAGAGTGATCATAGGGCCAGTACTCTGGAATCTTCTAACCTCCTAAAGCAACATAATGACCAGTCTAGGTTCTCT TTTGCTCGGCATGAAGAATCAAATAGTCAAGCGTATGACAATAGAAGTTACAGCATATATGGGCAGTTGTCAAGAGATCA GCCTCTTCAAGAGTTTGGAGCGAACCGTGATATGTATCAGGATAAGCTTGGCAGTCAAAATGGTTTTGCCTCAAACTATT CCGGTGGATATGAACAATTTGCTACTAGTCCTGGATTATCTTCTTACAAGAGTCCTGTGGCGAGGACTCAAGTTTCTGCT CCTCCCGGATTTTCTGCTCCCAACAGGTTACCTCCTCCCGGTTTCTCTTCACATCAAAGAGGCGACCTGTCTTCTGATAT TGCATCAGGAACTCGTTTACTTGACTCAGCCAATTTGTTGAGGAACGCATATCATGTACCACCTCCAAGTGGAAATTTGA ATGCTGCAGGAGATATTGAGTTTATTGATCCTGCAATTTTAGCTGTTGGAAGGGGAAGACTTCATAATGGGATGGAAACA GCAGACTTTGATTTGAGATCAGGGTTCTCTTCTCAACTGAATTCATTTGATAATGATGCAAGACTCCAATTGTTGGCGCA GAGGTCTCTGGCTGCACAACAAGTCAATGGATTTCACGATCCCAGGAACGTAAACAACTTCTCTTCTTCGTTCTCTGACC CATATGGGATTAGCTCGAGGCCAACGGATCAAACACAAGGAACTGGCTTGTCTCCTTTCACACAGCTGCCTAGACAAGCT TCAGCAAATCCATTGTTGTCAAATGGACACTGGGATAATAAGTGGAATGAACCACAAAGTGGTAACAATCTTGGGATTAC CCAGCTTCTAAGGAATGAACGGATGGGTTTTAATGACAACGTTTACAGCGGTTTTGAAGAGCCTAAATTCCGGAGGCCCG GTCCTGGAGATCCGTATAACAGAACATACGGGATTTGATTCTCGGAATTCTTCTTGGTTGGATTTTCACTTAAGACTGTC TCAAAAGAAGGATTTTTTGGCTGATTCCGGTGTTGGGAGATTCATAGTCGAAACTGC ........N...............................................................N....... ...............................N..................N......N..N......N............ ............................i............................................N...... ............................N.......................................i.....i..... ..............................................N......................i.......... ............................N..........................................N........ ........................................................................N....... ...............................N......................i......................... .....................N...................i........................N............. ...N..............N..................N.........................N...i............ .....N............................N...................................N......... ..............................................i....................N............ ......N......................................................................... ...........................................................i.................... ................................N...................................N........... ..............................N................................................. ................................................................................ ................................................................................ ............................................................N........N.......... ..........N......N...i........................N................................. ......................................N..................N...................... ......N.......................N........N........................................ ..........N.......................N............................................. ................................................................................ ....................N.........................................................N. ..................N........i.....................N.............................. ..N......................................N...................................... ............................................................i................... ..........N.......................N.......................N..................... ...............................N...........................i.................... .........N...................................................................... ................................................................................ .....................................................N.......................... N.................................................................N....N........ ....................................................N..N........................ ..........................N..................................................... ...N............................................................................ ......................N.......................N................................. ..............i.......i.........N............................................... ................................................................................ ......................................................... Pos Score Pred ------------------------ 9 0.283 - 73 0.103 - 112 0.132 - 131 0.116 - 138 0.338 - 141 0.350 - 148 0.351 - 189 0.601 Yes 234 0.366 - 269 0.331 - 309 0.798 Yes 315 0.826 Yes 367 0.256 - 390 0.784 Yes 429 0.235 - 472 0.200 - 553 0.189 - 592 0.358 - 615 0.534 Yes 662 0.304 - 682 0.589 Yes 707 0.483 - 724 0.233 - 739 0.259 - 758 0.357 - 784 0.443 - 788 0.536 Yes 806 0.205 - 835 0.220 - 871 0.296 - 927 0.625 Yes 948 0.400 - 967 0.347 - 1100 0.527 Yes 1153 0.317 - 1189 0.435 - 1231 0.238 - 1501 0.241 - 1510 0.103 - 1531 0.054 - 1538 0.455 - 1542 0.703 Yes 1567 0.262 - 1639 0.170 - 1658 0.475 - 1687 0.237 - 1711 0.307 - 1720 0.216 - 1771 0.176 - 1795 0.200 - 1941 0.371 - 1999 0.416 - 2019 0.477 - 2028 0.716 Yes 2050 0.246 - 2083 0.295 - 2122 0.157 - 2221 0.603 Yes 2251 0.241 - 2275 0.224 - 2299 0.188 - 2352 0.441 - 2380 0.553 Yes 2410 0.157 - 2614 0.193 - 2641 0.240 - 2707 0.394 - 2712 0.343 - 2773 0.355 - 2776 0.146 - 2827 0.413 - 2884 0.198 - 2983 0.328 - 3007 0.305 - 3055 0.511 Yes 3063 0.545 Yes 3073 0.475 -

Explain the output -- Processing locus 25: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGCACGGTAAAAGGAAGGATCTTTTGCTCGTCAATTGCTATATTAGCAGACCTTGCGAGGTGGGTTTAGGGTTTTGTTG TAAAAACATTTTTGGGGATAAGTTACGAAGATGACTTCTTTGCCTTCTTCAGTGATCAAATGGCGTTTTTTCCGCAGACA GATGCCTTCAGACGTTGTCTTCTCGCTCTGCTTACTCTGTTTCGCCTCCTGTCTCGCCGGAAAAATCACTGTTTTAGCTG ATTCGGACAAATCCGTGTTGCTTCGGTTTAAGAAAACGGTATCGGACCCTGGTTCGATTCTAGCTAGCTGGGTCGAAGAG AGTGAAGATTACTGCTCCTGGTTTGGTGTATCTTGCGATTCGAGCTCTCGAGTCATGGCTCTTAACATTAGTGGCTCTGG AAGTTCTGAAATTAGTCGAAATCGTTTCACTTGTGGTGATATTGGTAAGTTTCCGTTGTATGGGTTTGGTGTTCGGAGGG ATTGTACCGGAAATCATGGAGCTTTGGCGGGGAATTTGCCTTCTGTAATCATGAGTCTCACAGGACTTAGGGTTTTGTCT CTGCCTTTTAATTCGTTTAGTGGAGAGATTCCTGTAGGAATCTGGGGAATGGAGAAGCTTGAAGTTCTTGATCTGGAAGG GAATTTGATGACGGGGTCATTGCCGGATCAGTTTACTGGGTTACGGAATTTGCGGGTTATGAATTTGGGGTTTAACAGAG TTTCAGGTGAAATCCCTAATTCGCTGCAGAATCTGACAAAGTTGGAGATTTTGAATTTGGGTGGTAATAAGTTGAATGGA ACTGTTCCTGGTTTTGTTGGGAGATTCAGAGTGTTGCATTTGCCGTTAAACTGGCTTCAAGGCTCTTTGCCTAAAGATAT TGGGGATAGCTGTGGAAAGCTTGAGCATTTAGATTTGTCTGGTAATTTCTTGACTGGGAGGATTCCAGAGAGTTTGGGGA AATGTGCTGGTTTAAGGTCGTTGTTGTTGTATATGAATACGTTGGAGGAGACTATCCCTTTAGAATTTGGGAGTCTTCAG AAGCTTGAGGTTTTGGATGTTTCTAGGAACACTCTTAGTGGTCCATTACCTGTTGAACTTGGAAATTGCTCTTCGTTGTC TGTTCTCGTTCTATCAAATCTGTACAATGTGTATGAAGACATTAATAGTGTAAGAGGGGAAGCTGATCTTCCTCCAGGCG CTGATTTAACCTCGATGACCGAGGATTTCAATTTCTATCAAGGAGGAATCCCGGAGGAAATCACTAGGCTTCCGAAACTA AAGATACTTTGGGTGCCCAGAGCTACGTTAGAAGGTAGATTTCCAGGAGATTGGGGTTCATGTCAAAACTTGGAGATGGT TAATTTGGGTCAGAACTTCTTTAAAGGCGAAATTCCTGTCGGTCTTAGTAAATGCAAGAACCTTCGTCTTCTTGATTTGA GCTCGAATAGGCTTACTGGGGAGCTTCTCAAAGAGATTTCGGTCCCATGCATGAGTGTTTTTGATGTTGGTGGAAACAGC TTATCGGGTGTAATCCCTGATTTTCTCAACAATACCACAAGCCATTGTCCTCCTGTTGTTTACTTTGACAGGTTTTCTAT TGAATCTTACAGCGACCCGTCGTCTGTTTATCTCTCCTTCTTTACCGAGAAGGCTCAAGTTGGAACTTCCCTGATAGATC TTGGAAGTGATGGAGGTCCGGCCGTGTTTCATAATTTTGCAGACAACAACTTCACTGGTACTCTCAAGTCTATACCACTT GCACAGGAGCGGTTGGGAAAGCGAGTCTCTTACATATTTTCTGCTGGAGGGAATCGGTTGTACGGGCAATTTCCGGGAAA TCTGTTTGATAACTGCGACGAACTCAAAGCTGTTTATGTCAATGTAAGCTTCAACAAGCTCTCAGGTCGGATTCCTCAGG GACTAAACAACATGTGCACTTCTCTTAAGATTCTTGATGCCTCGGTGAATCAGATCTTCGGGCCAATCCCAACAAGTCTC GGGGATCTAGCTTCACTTGTTGCTCTTAATCTGAGCTGGAATCAGTTGCAAGGTCAGATACCGGGTAGCCTCGGGAAGAA GATGGCAGCTTTGACATATCTTTCGATTGCGAATAACAACCTCACGGGGCAAATTCCTCAGAGCTTTGGTCAGTTACATT CCTTGGATGTTCTTGATCTCTCTTCAAATCATCTATCTGGCGGTATTCCTCATGATTTCGTAAACTTGAAGAATCTCACT GTCCTGCTTCTCAACAATAACAACCTCTCTGGCCCAATCCCGTCGGGTTTTGCGACATTTGCTGTTTTCAATGTTTCCTC CAACAATCTGTCTGGTCCAGTTCCTTCAACCAATGGACTGACGAAATGCAGCACTGTTAGTGGAAACCCCTATCTTCGAC CTTGCCATGTGTTTTCTCTGACAACACCGTCTTCGGATTCCCGAGACTCCACGGGTGATTCTATCACGCAGGATTACGCA TCTTCGCCTGTTGAAAACGCTCCATCTCAGTCGCCAGGAAAGGGTGGATTCAACTCGCTAGAGATCGCCTCAATCGCATC AGCTTCAGCGATTGTCTCGGTACTAATTGCTCTTGTAATTCTCTTCTTCTACACAAGGAAATGGCATCCGAAATCGAAGA TCATGGCCACTACAAAACGAGAAGTCACTATGTTCATGGATATCGGAGTTCCAATAACCTTTGACAACGTGGTTAGAGCC ACAGGAAACTTCAACGCAAGCAATCTGATTGGAAATGGTGGATTTGGAGCGACCTACAAAGCAGAAATCTCTCAAGATGT GGTTGTCGCAATCAAACGCCTCTCGATCGGACGGTTTCAGGGAGTCCAACAGTTCCACGCAGAAATCAAAACTCTTGGTA GACTTCGACACCCGAACCTCGTGACTCTCATCGGTTACCACGCTAGCGAAACTGAGATGTTCTTGGTGTACAACTATCTC CCAGGAGGCAACCTCGAGAAATTCATCCAAGAAAGATCCACAAGAGATTGGAGAGTTCTTCATAAGATCGCTCTGGACAT AGCTCGAGCACTCGCTTACCTCCACGACCAATGCGTCCCACGGGTGCTTCACCGAGATGTTAAACCGAGTAACATCCTCC TAGATGATGACTGCAATGCGTATTTATCAGATTTCGGGTTAGCGAGATTGCTTGGGACATCGGAAACGCACGCAACAACT GGTGTGGCGGGTACGTTTGGGTATGTAGCTCCCGAGTACGCAATGACATGCAGGGTATCAGACAAAGCAGATGTATACAG CTACGGAGTGGTGCTTCTAGAGCTGCTTTCGGACAAGAAAGCACTTGATCCGTCATTTGTGTCGTATGGTAATGGTTTCA ACATAGTGCAATGGGCTTGTATGTTGTTGAGACAAGGAAGGGCAAAGGAGTTTTTCACAGCGGGATTGTGGGACGCAGGT CCGCATGATGATCTAGTAGAGGTTCTGCATTTAGCGGTTGTTTGTACGGTGGATTCTCTGTCGACGAGGCCGACGATGAA GCAAGTTGTAAGACGGTTGAAGCAGCTACAACCTCCGTCGTGTTAGCACCTCCCTCCGCAGGATTTGATTATTATTCTTT CTTTAAAAAAAGTTCTCTCAGTTGTAAACTTGTATGTGTACGTAGTAGTTGTTAGAAGAAGAAAG ................................................................................ ..............................N............................i.................... ................................................................................ ......................................................i......................... ...........................................................N.................... ...............N..................................N............................. ................................................N............................... .......N..................................................N..................... ...........................................................................N.... ................................................................................ ................................................................................ ................N............................................................... ..........................i.....N............................................... ..............N................................................................. ...........................................................N...............i.... ...................................................N............................ ..............................................N...N............N................ ................................................................................ ................................................................................ .........N...................................................................... ................................................................................ ...................................N.....N...................................... ...........i........................N........................................... ................................................................................ ......N............................................N............................ ......................................................................N......... ................................i............i.................................. ......N......................................................................... ................................................................................ ............................................................i................... ..i..........................i.....i............................................ ..................................N.........................................i... ................................................................................ ........................................................N....................... ................................................................................ ..............................N.........................N....................... ...N..N........N................................................................ ......................N...................N....N......................N......... .................................................................N.....N........ ..........i.........N........................................................... ....N..N...................................................................N.... ................................................................................ .................................N............................... Pos Score Pred ------------------------ 111 0.282 - 140 0.592 Yes 162 0.509 Yes 375 0.692 Yes 460 0.114 - 496 0.165 - 531 0.466 - 609 0.273 - 648 0.275 - 699 0.144 - 796 0.362 - 962 0.334 - 993 0.464 - 1057 0.115 - 1147 0.582 Yes 1153 0.342 - 1215 0.424 - 1340 0.128 - 1356 0.732 Yes 1412 0.212 - 1487 0.096 - 1491 0.441 - 1504 0.154 - 1690 0.244 - 1876 0.181 - 1882 0.159 - 1932 0.732 Yes 1957 0.369 - 2082 0.510 Yes 2167 0.109 - 2212 0.292 - 2311 0.141 - 2353 0.635 Yes 2366 0.657 Yes 2407 0.175 - 2621 0.801 Yes 2643 0.891 Yes 2670 0.737 Yes 2676 0.651 Yes 2755 0.460 - 2797 0.710 Yes 2937 0.336 - 3071 0.345 - 3097 0.383 - 3124 0.415 - 3127 0.330 - 3136 0.164 - 3223 0.170 - 3243 0.258 - 3248 0.469 - 3271 0.189 - 3346 0.122 - 3352 0.248 - 3371 0.631 Yes 3381 0.378 - 3445 0.154 - 3448 0.115 - 3516 0.193 - 3634 0.057 -

Explain the output -- Processing locus 26: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTACTTTCTGGCATTTGCTTTCTGATTTGACTTCACCACTTCGTTTCGGAAAGTTCTTAATTATTTTTCCGAAATCCCAA AATTGTATATATTTGGTTTAATTTTTATGTTTTTGTTTTCTGAAAATGTTGAATTGTTTGTTGATTATGAAGATGGATCG TAAGAGTTGGCCATGGAAGAAGAAATCCTCTGAGAAAACAGCTACTGTCACAGAAGTTGTTGATCAGGAAAATGGGAAGA AGCCGAGTTATATCCAGATCTCATTTGATCAATACACGAATCTAAATGGTTTAAAAGATGAAGTCAAGAGCTACGAAGAA AAAGTTACAAAGCTTGAGGATCAGATCAAAGATTTGGATTTGAAGCTATCAACAGCTAATGCAGATATTGTAGCAAAGGA GGTTTTAGTCAAACAACACTCCAAAGTTGCTGAAGAAGCTGTCACAGGTTGGGAAAAAGCTGAAGCAGAAGCATCAGCTT TGAAAACACATCTCGAAACCATTACTCTCGCAAAGCTCACTGTCGAAGATCGAGCAGCGCATTTAGATGGAGCGTTAAAG GAGTGTATGAGACAAATAAGGAGTTTAAAGGAAGAGAATGAACAGAAGCTGCATGATGTTATCGCTACTAAAACCAACCA AATGGACAATCTTAGAGCTGAGTTTGAGTCTAGGATTGGGGAATACGAGGAAGAACTGCTTCGGTGTGGTGCTGAGAATG ACGCGCTTTCGAGATCGTTGCAAGAGAGGTCTAACATGCTAATGAGGATAAGTGAGGAGAAGTCTCAAGCAGAGTCTGAG ATTGAGCATCTTAAGAACAATATAGAGTCTTGTGAAAGGGAGATAAATACTCTCAAGTATGAAACTCATGTGATTACCAA AGAGTTGGAGATCCGTAACGAGGAAAAGAATATGAGTATGAGATCTGCGGAAGCGGCGAATAAGCAGCATTTGGAAGGTG TCAAGAAGATTGCAAAGTTGGAAGCTGAGTGTCAAAGATTACGAACTCTTGTGAGAAAGAAACTTCCTGGTCCTGCAGCA CTTGCACAGATGAAAATGGAGGTTGAGAGTTTAGGATTTGGAGATCATCGTCAAGATCATCGACAAAGAAGGTCACCGGT TAGACCATCTAGTCCTCTTATGTCTCCAATGTCACACATGTCTCAGGTTTCTGAGTTCTCACTTGACAATATGCAAAAGT TCCATAAAGAAAACGATTTGCTTACTGAGAGGCTACTTGCTATGGAAGAAGAAACAAAGATGCTTAAAGAAGCTTTGGCT AAGCGTAACAGCGAGCTTCAAGTGTCGAGAAATCTCTGTGCTAAGACAGCTAATAGGCTACAAACCTTGGAAGCTCAAAT GATGAGTAAAAGTCCAACCAAGAGAGGTTTTGAAATGCCAGCTGAAATCTTTTCTCGTCAAAATGCTAGTAATCCTCCTA GTATGGCTTCAATGTCTGAGGATGGAAACGAAGATGCTAGAAGCGTTGCTGGCTCTTTGATGTCTGAACTCTCTCAATCT AACAAGGATAAAGCCAACGCGAAGATCAAGAAGACCGAGAGTGCTAACCAGTTGGAGCTGATGGATGATTTTCTTGAAAT GGAAAAGCTAGCTTGTCTACCAAATGGCTCAAACGCAAATGGTTCGACGGATCACTCAAGTGCTGATTCCGATGCTGAGA TTCCACCAGCGACTCAACTGAAGAAAAGAATCTCCAATGTGCTTCAGTCTCTCCCTAAAGATGCTGCATTTGAGAAGATT TTGGCAGAGATACAATGTGCAGTTAAAGATGCAGGTGTTAAGCTGCCTTCTAAGAGTCATGGAGCTAATTTGAACGGTCT TACAGAAGAAAAGGTGATAGCCATGTCAAATGAGACTACAGAGGAAAAAGTTACCATTGTCGAGGTAATAACACAAGAAT TGTCTGATGCTCTTTCTCAGATATACCAGTTTGTTACCTATCTTTCAAAGGAAGCAACGGCGTGTTCTGAGAACAGAACT TTCTCCCAAAAAGTTCAAGAATTCTCTACCACATTCGAGGGAGTCTTGGGCAAGGAAAAGACATTGGTCGATTTTCTGTT TGATCTTTCGCGAGTTTTAGTCGAAGCTAGTGAGCTAAAGATCGATGTGCTAGGGTTCCACACATCCACAGTAGAGATTC ACAGCCCGGATTGCATTGATAAAGTCGCTCTACCGGAAAACAAAGCACTTCAGAAAGATTCATCAGGTGAACATTACCAG AATGGTTGTTCTCAGAGCTCAGATTCTGAGATTCCGGATGATTGCAACGGAACATCTGGCTATGAACCAAAGCTTGCAAC TTGTAAATTTACAACAGAGGAGTTTGAAGGTTTGAAACTCGAGAAAGAAAAAGCAGAGAGCAACCTTGCAAGCTGTGAAG CAGATCTTGAAGCAACTAAGACCAAACTACAAGAAACAGAGAAACTTCTTGCTGAAGTCAAATCAGACTTGGAATCTGCT CAGAAGTCTAATGGCATGGGCGAGACTCAACTCAAATGCATGGTGGAATCATATAGATCTCTTGAAACTAGATCATCGGA GCTGGAAATCGAGTTGACTTCTCTTAAAGGCAAGATCGAAAACTTAGAAGATGAGCTTCACGACGAGAAGGAAAATCATC GAGAAGCTTTAGCCAAATGCCAAGAACTCGAGGAGCAATTACAAAGGAACAACCAAAACTGCCCGAATTGTTCAGTAATC GAAGATGATCCTAAAAGCAAACAGGACAATGAACTAGCGGCTGCTGCAGAGAAGCTAGCAGAGTGTCAAGAGACTATATT ACTTCTCGGGAAGCAACTCAAATCCATGTGTCCTCAAACAGAACAGGTTGCTTCTTCTCCAAGCCAAGAACAACAAGCCC TAAACCCTGAAGAAGAGGAATACGCAACTTCCACAAATCCTCAAGACAGCAAATTGAGTTCGCCTTCAGACAAGGATACA CCATCGATGAATACAATGAAATCTCCGGTCGCGTCGAAACATAGACACACGAAGTCGAACTCGTCATCGTCCTCATCAGG ACTTACTCCTGAGAAACACTCTAGAGGATTCAGCAGGTTCTTCTCCACTAAAGCAAAGTAAAAATAAATAAAAAGAATTA AAAAAAGTATAAATCTAACTATGTTGTATATCGTATCTATTGCTACTATATTATATGGGTTTTGTTTTTAATTTTTTACT ................................................................................ ..........................N..................i....................N.....i....... ............N..........................................................N........ .............................................N...........N...................... ..........................................................N..................... ................................................................................ ..................................................................N............. ......N..............................N..............N..N........................ ...................................N.....i...................................... ..........................................................N........N............ ...............................N.....i.......................................... ................................................................................ .........N.....i................................................................ ...................N........N........i................................N......... .........................................i.................i.................... ..............................................................................i. ..i........i.........N...........N.........................N.................... ............................................................i...N.............i. .......................N..............N................................N........ ....................................N.......................N................... ..............i.............N.............................N..................... ......................N......N.................................................. ......N......................................................................... ................................................................................ ............................................N................................... ................................................................................ ................................................................................ ................................................................................ ..........N....i...................i...N........................................ ..................................................i............................. ................N............................................................... ....N.......................N................................................... .........................i...................................................... ................................................................................ ......i........N................................................................ ................................................................................ ....................N.................................N......................... Pos Score Pred ------------------------ 107 0.301 - 126 0.700 Yes 147 0.434 - 153 0.594 Yes 173 0.340 - 232 0.401 - 286 0.231 - 298 0.123 - 379 0.123 - 547 0.339 - 567 0.372 - 598 0.457 - 613 0.270 - 616 0.221 - 642 0.693 Yes 718 0.184 - 756 0.418 - 762 0.527 Yes 859 0.096 - 868 0.155 - 912 0.472 - 918 0.546 Yes 1050 0.165 - 1056 0.538 Yes 1140 0.369 - 1149 0.447 - 1158 0.658 Yes 1191 0.402 - 1242 0.609 Yes 1260 0.543 Yes 1359 0.680 Yes 1362 0.437 - 1395 0.837 Yes 1423 0.108 - 1443 0.850 Yes 1452 0.806 Yes 1462 0.235 - 1474 0.140 - 1500 0.413 - 1581 0.609 Yes 1585 0.183 - 1599 0.663 Yes 1624 0.435 - 1639 0.186 - 1672 0.072 - 1717 0.217 - 1741 0.190 - 1775 0.692 Yes 1789 0.442 - 1819 0.152 - 1863 0.409 - 1870 0.118 - 1927 0.346 - 2125 0.148 - 2242 0.444 - 2278 0.386 - 2302 0.119 - 2491 0.383 - 2496 0.556 Yes 2516 0.527 Yes 2520 0.499 - 2611 0.622 Yes 2657 0.321 - 2725 0.310 - 2749 0.319 - 2826 0.623 Yes 2967 0.653 Yes 2976 0.425 - 3141 0.120 - 3175 0.043 -

Explain the output -- Processing locus 27: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCTGTTTCTTCTTGTGTCATCGTCAAATCAAATCCAATCACTAACAGTAGAAACTGTTTCTCTACTGGGTATTTTTTT TTGGCAACCCTTAAATTCTGAAGTAAGAGAGAAGAAGAAGAAAGTGTGAGATTCCGACGAACGGGAAGAAAAATGATAAA TTTTCGCAGCATGAATCAAGCTTCTTCGAGGATAAGGATCGCTCTTTCGGGTCGGATTGAAACAGCTTTAGAGAATATCT ACAGAAAACACAATCTTACTCCGATTAATGATGAGACGAGACAGAGACTGTCTTCGATTCCTGAGAATTTGGGTTTTGAG TTGGTCAGGAAAGTTTTTAGTTTGCAGGCTGGTTTGATATACAACCTCGACAGCTTCATCGTTTCTAAGGTTAACCAAGC CGTTAGTTTTACTGGCTATCCACAGCCGGCGAACTCTCTTAGCCCCTCTGGAAGACACGTGTCCAGGGTTCTTCAAGAAG AGATGTCTGTTGATTCTGATGCTCCTTCTCCTAAGTCTTTGAAAAGTGAAGACAAAGGAGGATCTCTGCACATTCCCCAG CTGGTAGCTTTGGGTGAACTTGAATTTAAGAAGGTGTTTTTGTTACTTAGCTATATTCCAGGGCAACATGTGGGCCAGGT TATAACTGCTGATGAGATTAGACTGTGGAAAGATTTGCCAATGGTTGAATATGAAGCAGCGGTTTGGGACCGCTTGGGCC GGCATTATTGTCCCCAAAAGGACCGAAGAATGTTGCAATGGGATAGCGGGAAGACCCATTACTATCAGTGTAATGTTGCT CCCAATGGTAGCTACACATTCAAGGTTCTTTCAGCTCTTCAAGGCCCTCTTCTTGAACACACTGGAACACACTTGCACAA GGTCTTGGGTGATGATAATGTTTTAACCGTCAAATTCGCGGATGTCCAGAAAAGTTCATCAACATATAGCATTGACCATT ACTTTACATACAAAGGGATTGCCAAGAACGGTATCATGATTGGTTTACGTCGTTATCAATTTTTTGTTTTCAAAGATGGA GGAAAAGAGGAAAAGAAGAAAGATCTTTCTACAAAGAAAGTGAAATGCTACTTTATACGCACGGACTCAACAGCTTTTTA TGATATGCAAAATCCCTATATTTTAACCGGAAAGTCAATTTATGAAGCTCGTATGCATTTTATGCACGTGCATCGAGCAC CAACTTTGGCGAATTATATGGCAAGGTTTTCCTTAATTCTGTCAAAGACTAAAACGCTTGAAGTTGACATGACTGGGATT ACCTTTGATCAAATTGATGATATACACTGCCATGATCAAGATGGGAAAGATGTTCTTGACAAGAACAAAAAACCTTGCAT ACATTCAGATGGAACTGGCTACATCTCTGAGGACCTGGCTCGGATGTGTCCCCTAAATATATTTAAAGGGAAATGTCTCA GAAGTGAAAGTATTCAGGAAGCATGTTACCAAGACCCGCCTCTTTTGATCCAGTTTCGGATGTTTTATGATGGTTATGCT GTTAAGGGAACTTTTCTCTTAAACAAGAAACTTTGTCCTCGGACTGTCCAGGTTCGACCTTCGATGATCAAGGTGTCTAA AGATCCATCCTTGTCCAATTTTAGCACCTTTAACGCCTTGGAAGTAGTAACCACAAGTAATCCACCAAAAAGAACAAAGC TATCAAAAAATTTGGTTGCGCTGCTTAGCTATGGAGGAATCCCTAATGAATTCTTTTTGGATATATTGCTCAACACGTTG GAAGAGTCTAAAAGCATATTCTACAACAAACGTGCTGCACTTAATGCTGCTCTTAATTATGGGGAAATGGACGACCAGAA CGCCGCACAGATGATATTGGTTGGTATCCCCCTTGATGAACCACACTTGAAGAATTATCTGTCTATACTTTTAAAGACAG AGAAAAATGATCTCAAAGCAGGAAAACTTCCTGTGACCGAATCATACTATCTTATGGGAACAGTGGATCCCACCGGAGCG CTAAAGGAAGATGAAGTCTGTGTAATTCTTGAGTCTGGGCAAATTTCGGGAGAAGTTTTAGTGTATAGAAATCCTGGATT ACATTTTGGAGACATACATATTCTTAAGGCCACATATGTCAAGGCCTTGGAAGAGTATGTTGGAAATTCCAAGTTTGCTG TGTTCTTCCCTCAGAAAGGTCCAAGATCCTTGGGGGACGAGATTGCAGGTGGTGACTTTGATGGTGATATGTATTTCATA TCAAGAAACCCTGAGCTACTTGAAAACTTCAAACCAAGTGAACCGTGGGTGAGTTTGACTCCTCCCAGTAAAAGTAACTC TGGTAGAGCGCCAAGCCAGCTTTCCCCAGAAGAGTTGGAAGAAGAACTTTTCGAAATGTTCCTGACTGCAGGATTTCATG CCAGCAATGTTATAGGGATAGCTGCAGATAGCTGGTTAACAATAATGGACCGCTTCCTCATACTAGGAGATGACAGGGCT GAGGAAAAAGCTGAAATGAAGAAGAAGATGCTTGAGCTGATTGATATATACTATGATGCTCTTGATGCACCAAAGAAAGG GGATAAAGTCTACCTCCCAAACAAGCTGAAGCCTGACATCTTTCCACATTACATGGAGCGGGATAAAAAATTCCAGTCCA CTTCTATTCTTGGATTAATCTTTGACTTTGTTAAATCACAGACAACAGAGGAACCCTCACCATCATCTGAGATCAGTAAA CTCCCGTGTTTCGAAGATGAGCCGGTCTCCGAGTTTCATATGCAGAAATGTAGACTGTGGTATGATAATTACAGAACTGA GATGACACAGGCGATGAAGACTGATAAGGATGAATCAGCCAATGAAGTTATTCAGAGATACAAGCAGGAGTTCTATGGCG CTGCAGGGTTTGAAGACAGCAAGAAAAGTCTGGAAGAACTCTATCCGCAAGCTCTCGCACTCTACAAGATCGTTTACGAT TATGCCATTCATGCAGGTGTTTCGAAATGCAGGTTTGTCTGGAAGGTAGCAGGACCGGTACTGTGCAGATTCTATCTAAA TAAGAAAATGCAGGAGAAATGTTTAGTGTGTGCACCATCTGTGCTTAAAGAGCTTTGGGGTTGAAGAATACAAGCCTTTG TTGTTGCTACATGTCTAATAATAAGAAGCGTATGAGGCAAGTAAGTTAACTAATAGGTGCCAAGATGTGTGTGTTGTAGT GCC ................................................................................ ........................................................................N....... ..........N..................................................................... ...........................N..N................................................. ................................................................................ ................................................................................ ..i...............N............................................................. ...................................................................N............ ...........i............................i.........i............................. .............................N.......N..................................N....... ....N........................................................................... ...........N.....N.......................N...................................... ...................................N.......................................i.... ............................................N..................................N ....i....................................N..........N........N.................. .................i..................................................i........... ................N..............i........N........N.............................. ........i..................................N............................N....... ......................N....................................N......N..N.....N.... ...............................................................N................ ................................................................................ ..............................N..............N.................................. ...........................................N..............N.......i............. ..........i........................N............................................ ......N..............................................i.......................... ..........N..................................................................... ...................................N....................N....................... ............................................................N.......i........... ................................................................................ .......................................................i.....................N.. ......N.....................................i........................N.......... ...............i...........N........................N..N........N............... ....................................................N........................... ................................................................................ ................N......................N.......N.............N.................. ................................................................................ .......i..........i............................................................. ..........N....................N................................N............... ... Pos Score Pred ------------------------ 153 0.497 - 171 0.273 - 268 0.197 - 271 0.332 - 483 0.788 Yes 499 0.088 - 628 0.478 - 652 0.585 Yes 681 0.643 Yes 691 0.667 Yes 750 0.264 - 758 0.399 - 793 0.294 - 805 0.192 - 892 0.269 - 898 0.344 - 922 0.165 - 996 0.463 - 1036 0.588 Yes 1085 0.248 - 1120 0.114 - 1125 0.519 Yes 1162 0.355 - 1173 0.180 - 1182 0.328 - 1218 0.673 Yes 1269 0.598 Yes 1297 0.168 - 1312 0.555 Yes 1321 0.120 - 1330 0.242 - 1369 0.584 Yes 1404 0.362 - 1433 0.339 - 1463 0.392 - 1500 0.416 - 1507 0.134 - 1510 0.198 - 1516 0.261 - 1584 0.375 - 1711 0.485 - 1726 0.177 - 1804 0.156 - 1819 0.277 - 1827 0.668 Yes 1851 0.609 Yes 1876 0.127 - 1927 0.377 - 1974 0.634 Yes 2011 0.218 - 2116 0.310 - 2137 0.247 - 2221 0.304 - 2229 0.736 Yes 2376 0.728 Yes 2398 0.251 - 2407 0.132 - 2445 0.621 Yes 2470 0.330 - 2496 0.667 Yes 2508 0.409 - 2533 0.138 - 2536 0.143 - 2545 0.227 - 2613 0.450 - 2737 0.401 - 2760 0.259 - 2768 0.488 - 2782 0.444 - 2802 0.781 Yes 2814 0.771 Yes 2830 0.382 - 2842 0.174 - 2875 0.315 - 2962 0.150 - 2971 0.325 - 2987 0.587 Yes 3048 0.617 Yes 3059 0.660 Yes 3131 0.043 - 3152 0.060 - 3185 0.062 -

Explain the output -- Processing locus 28: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGATTACCTTTTTATGGATTAAGTCTGCACAATGTTGCCTTATATGAACTTTTTTCCATAGATCTGATAGATTTGGGGG AGAATTTAAGCTTAGAATTAAGTTGATCTATTAGATCTGATGAAAAAATTGGGAATATTTTGTAGGGAAATGACTATAAA AACACCGGGAACTCCGGTTTCGAAGATGGACAGAACACCAGCTGTAACACCAGGTGGAAGCTCAAGATCCAGGGAAGAGA AGATTGTTGTTACTGTAAGGTTAAGGCCAATGAATAAGAGAGAACTGTTAGCTAAAGACCAAGTTGCTTGGGAATGTGTA AATGACCACACCATTGTGTCTAAACCACAAGTTCAAGAACGTTTGCATCATCAGTCCTCATTTACATTTGATAAAGTTTT TGGACCAGAGAGTTTAACAGAGAATGTGTATGAAGATGGTGTTAAGAATGTTGCATTGTCTGCTTTGATGGGCATAAATG CAACAATATTTGCATATGGACAAACAAGCAGTGGAAAGACATATACCATGAGAGGAGTAACGGAGAAAGCTGTCAATGAT ATTTACAATCACATAATTAAGACCCCTGAAAGAGATTTTACTATCAAGATTTCTGGTCTGGAAATATATAATGAAAATGT CAGAGATCTGCTCAATTCAGATTCGGGACGTGCCCTTAAACTCCTTGATGATCCAGAGAAAGGTACTGTGGTTGAAAAAC TGGTTGAAGAAACAGCTAATAACGATAACCATTTGCGGCATCTGATTAGCATTTGTGAAGCTCAAAGACAAGTTGGAGAA ACTGCTTTGAATGATACTAGCTCGCGGTCACACCAAATAATCAGACTGACAATACAAAGTACTCACCGCGAAAATTCAGA TTGTGTGAGGTCATATATGGCTAGTCTGAACTTTGTGGACTTAGCAGGAAGCGAAAGAGCATCACAATCGCAGGCAGATG GAACGAGACTCAGGGAAGGTTGTCATATCAATCTAAGTCTAATGACACTTACAACTGTCATAAGGAAATTAAGTGTGGGG AAAAGAAGCGGCCATATACCTTACAGAGACTCCAAGCTTACTAGGATTTTACAGCATTCACTAGGTGGGAATGCTCGAAC AGCCATTATATGTACATTGAGTCCAGCTTTGGCTCATGTTGAACAATCAAGAAATACTCTATACTTCGCCAACCGAGCCA AGGAAGTTACAAACAATGCCCACGTGAACATGGTTGTCTCTGATAAGCAACTAGTGAAACATCTTCAGAAAGAAGTGGCT AGATTGGAGGCCGAGCGTCGCACCCCTGGTCCATCGACAGAAAAGGATTTCAAGATCCAGCAGATGGAAATGGAAATCGG AGAGCTTAGAAGACAAAGAGATGATGCACAAATTCAACTTGAGGAGTTACGCCAAAAGCTTCAAGGGGATCAGCAACAAA ATAAGGGCTTAAATCCTTTTGAATCTCCAGACCCACCGGTCAGAAAATGTCTTTCCTATTCTGTTGCCGTAACGCCTAGC TCGGAGAACAAAACGCTAAACCGGAATGAGAGAGCAAGAAAGACTACGATGCGCCAATCTATGATACGGCAATCATCAAC TGCTCCATTTACATTGATGCATGAAATCCGCAAACTCGAACACCTTCAAGAACAGTTAGGGGAGGAAGCAACGAAAGCCC TTGAAGTGTTACAAAAGGAAGTAGCTTGCCATAGACTAGGGAATCAAGATGCAGCTCAGACGATAGCTAAGCTTCAAGCA GAGATAAGGGAAATGCGTACTGTTAAACCATCTGCAATGCTTAAAGAGGTTGGAGATGTCATAGCTCCTAACAAGAGCGT AAGTGCCAATCTCAAGGAAGAGATAACAAGGCTCCATTCACAAGGAAGCACCATTGCGAATCTAGAGGAGCAACTTGAGA GCGTTCAGAAGTCAATAGATAAGTTGGTGATGTCTCTTCCGAGCAATATCAGTGCTGGAGATGAGACTCCAAAGACAAAG AATCATCATCATCAATCAAAGAAGAAGAAGCTTCTTCCTCTGACACCAAGCAGCGCATCCAACCGCCAGAACTTCTTGAA ATCTCCATGCTCTCCGCTTTCAGCTTCTAGGCAAGTCTTGGATTGTGATGCTGAGAACAAAGCTCCTCAAGAAAACAACA GTTCTGCAGCTAGAGGGGCGACGACACCACAGGGGTCTGAGAAAGAGACTCCACAGAAAGGAGAAGAAAGCGGAGATGTC TCGTCAAGAGAAGGTACTCCAGGCTACAGAAGATCAAGCTCAGTGAACATGAAGAAAATGCAGCAAATGTTTCAAAACGC AGCAGAGGAGAATGTAAGAAGCATAAGAGCATACGTAACCGAACTCAAAGAACGAGTAGCTAAACTACAGTACCAGAAAC AACTTCTCGTTTGTCAGGTGCTTGAGTTGGAAGCAAATGATGGAGCTGGATATAGTGTAGAGAACGAGGAGAACACGATA ATGGAAGATGAAGAGCAGAATCAAGTGGCGTGGCATATAACTTTCATAGAGGAAAGACAACAGATCATAGAGCTGTGGCA TGTCTGCCATGTCTCCATAATCCACAGGACACAATTCTACCTACTGTTCAAAGGTGACCAAGCCGATCAAATCTACATGG AAGTCGAGCTAAGACGGTTAACTTGGTTAGAACAACATCTAGCAGAAGTAGGAAACGCAACTCCAGCTCGAAACTGCGAT GAATCTGTGGTTTCTCTATCATCAAGTATAAAAGCATTGAGAAGAGAGCGAGAGTTTTTGGCGAAAAGGGTTAACTCGAG ATTGACGCCTGAGGAGAGAGAGGAGCTGTATATGAAATGGGATGTGCCATTGGAAGGGAAACAGAGGAAACTTCAGTTCG TGAACAAGCTATGGACTGATCCTTATGATTCAAGGCACGTGCAAGAGAGTGCAGAGATAGTTGCGAAGCTAGTTGGATTC TGTGAAAGTGGCAACATCTCTAAAGAGATGTTTGAGCTCAATTTCGCAGTGCCTTCAGATAAAAGACAGTGGAACATTGG TTGGGACAACATCTCTAATCTTCTTCACCTATGAAGACTTATTGTGTGTTTATGATTTGTAAAAGTACACGACACTTCTT TGTCTCCTCTGAATTTGATTAAATAAGCTTTACCAGACTCGTCTGATTATGTT ..............N.................i...........N................................... .......................................N.............................N.......... .........................N...................................................... .............................N...........................................N...... .......................N.....N.....N...........N...................N.........N.. ...............N...............................N...........................N.... ......................................................................N.....N... ...............................................N................................ ................................................................................ ..........N..................................................................... ................i............................................................N.. .........................................N...................................... ......................................................................N......... .........i.........................N............................................ ...............N.............i.................................................. ...............................................................i.....N.......... ....................N..i........................................................ ..............................................i................................. .........................N......................N...........N................... ................N...N........................................................... ................................................N............................... ............i.......................i..................N........................ ................................................................................ .............................i..............................N................... ................................................................................ ......N........................................N................................ ...........................................................................i.... ................................................i........N........N............. ...........N.................................................................... ....................................N..N........................................ N......N.......................................................................N ........N...................................................................i... ..............................................................................N. ................................................................................ ...............................N....i....N...................................... ..........N.............N....................................................... ...........................N.................................................... ..............................N....................N............................ ................................................N.... Pos Score Pred ------------------------ 15 0.370 - 33 0.505 Yes 45 0.188 - 120 0.419 - 150 0.398 - 186 0.422 - 270 0.391 - 314 0.201 - 322 0.464 - 424 0.256 - 430 0.249 - 436 0.462 - 448 0.269 - 468 0.405 - 478 0.112 - 496 0.191 - 528 0.313 - 556 0.115 - 631 0.449 - 637 0.452 - 688 0.080 - 811 0.119 - 897 0.605 Yes 958 0.436 - 1002 0.294 - 1111 0.189 - 1130 0.505 Yes 1156 0.338 - 1216 0.496 - 1230 0.695 Yes 1344 0.502 Yes 1350 0.446 - 1381 0.479 - 1384 0.521 Yes 1487 0.774 Yes 1546 0.457 - 1569 0.445 - 1581 0.328 - 1617 0.255 - 1621 0.167 - 1729 0.147 - 1773 0.550 Yes 1797 0.631 Yes 1816 0.285 - 1950 0.500 Yes 1981 0.269 - 2087 0.301 - 2128 0.296 - 2236 0.515 Yes 2289 0.574 Yes 2298 0.489 - 2307 0.383 - 2332 0.422 - 2437 0.255 - 2440 0.475 - 2481 0.470 - 2488 0.320 - 2560 0.438 - 2569 0.186 - 2637 0.654 Yes 2719 0.145 - 2832 0.150 - 2837 0.566 Yes 2842 0.205 - 2891 0.368 - 2905 0.140 - 2988 0.381 - 3071 0.164 - 3092 0.071 - 3169 0.054 -

Explain the output -- Processing locus 29: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACCAGAAGGTGTTGCCGTTGAAGATATTTACATGGATGAAGCTTCTGGACTCACTTCTCATAAGAAACATGACCTTTTAC TTGTTCCTGTGAAATCTTTAGTGTAACACTTGTGTAAGTAAACATGACCTTTTACTTCTCATAAGTAATGTAATGGTTAA ACCAAATAAACCGGTTCAATCAGAATCAAACCGGTTCACTGTTGTAAACCGACTCTTCTATATTCGTTTAGTTTCCTTAG CAAGAGAAAGGAGACGCCTTTTTTGCGAAATCTCCATGAAGCTTTATCCTCGAACTTCTTCAGGACTCTTTTCTCTCCAA TCTCTTCTCAAAAGTGGCTTCTCTCCAACGTTAAACTCTATCGACAGATTTCTTCGATATCTCTACCGTCTTCAGAAGTT TAATTGCATTCTTCAATTCTACTCTCAGCTAGATTCGAAGCAAATCAACATCAATCACCGGATTTACTCAATCGTTTCAT GGGCATTTCTCAATTTGAATCGATACGAAGACGCAGAGAAGTTTATCAACATCCATATTTCAAAAGCTTCGATTTTTCCC AGGACCCATATGTTAGATTCTCTAATTCATGGATTTAGCATCACACGTGATGATCCAAGTAAGGGTCTTCTGATTCTCCG AGATTGTTTACGGAATCACGGTGCGTTTCCTTCTTCCTTAACGTTCTGTTCACTGATTTATCGGTTCGTTGAGAAAGGAG AGATGGATAATGCTATTGAGGTTTTAGAGATGATGACGAACAAGAATGTAAATTACCCATTTGATAATTTCGTATGTAGT GCTGTGATTTCTGGGTTTTGTAAGATTGGTAAACCGGAACTTGCGTTAGGGTTTTTCGAAAGTGCTGTCGATTCAGGTGT TCTAGTGCCGAACCTTGTAACTTATACTACGCTTGTGAGTGCTCTTTGTCAGTTAGGTAAGGTTGATGAAGTTAGGGATT TAGTTAGGAGATTGGAAGATGAAGGATTTGAGTTTGACTGTGTGTTCTATAGTAATTGGATTCATGGGTACTTTAAGGGA GGTGCATTGGTGGATGCACTTATGCAGGACAGGGAAATGGTGGAGAAGGGAATGAATCGAGACGTTGTTAGCTATTCGAT ACTAATAGATGGGTTGTCGAAGGAGGGGAATGTTGAAGAAGCGTTGGGGTTATTAGGGAAGATGATAAAGGAAGGAGTAG AGCCGAATTTGATTACTTATACAGCTATTATTAGGGGACTCTGCAAAATGGGTAAACTTGAAGAGGCATTTGTGTTGTTC AATAGAATTCTAAGTGTTGGAATCGAAGTGGATGAGTTTCTGTATGTGACTTTGATAGATGGAATTTGTAGGAAAGGGAA TTTGAATCGAGCTTTCTCTATGTTGGGGGATATGGAGCAGAGAGGGATACAACCAAGCATTCTTACGTATAACACTGTGA TTAATGGACTTTGCATGGCAGGGAGAGTGTCGGAGGCAGATGAAGTTTCAAAAGGAGTTGTGGGAGATGTCATTACTTAT AGTACTTTATTGGATAGTTATATAAAGGTGCAGAATATTGATGCTGTATTAGAGATTAGGCGTAGATTTTTAGAAGCTAA AATCCCTATGGACTTGGTTATGTGCAACATTCTCCTGAAAGCATTTCTATTGATGGGTGCATATGGAGAAGCTGATGCAC TTTACAGGGCAATGCCAGAAATGGATCTGACTCCAGATACAGCCACATACGCAACGATGATTAAGGGGTATTGCAAAACG GGTCAAATCGAAGAGGCACTTGAGATGTTTAATGAGTTGAGGAAAAGCTCAGTTTCTGCAGCTGTATGTTATAATCGCAT TATTGATGCACTCTGCAAAAAAGGGATGCTGGACACAGCCACAGAAGTTCTTATCGAGCTGTGGGAGAAAGGTTTATATT TGGACATCCATACATCTCGGACCTTGTTACATTCAATCCATGCTAACGGAGGTGACAAAGGAATACTCGGTTTGGTTTAT GGACTGGAACAGTTGAATTCAGATGTTTGTCTTGGGATGCTCAATGATGCTATCTTACTTTTATGCAAGAGAGGTTCTTT CGAGGCAGCGATTGAAGTTTATATGATTATGAGGAGAAAAGGTTTAACTGTTACTTTCCCTTCTACAATTCTAAAAACAC TAGTGGACAATCTCAGATCATTGGATGCTTATCTACTCGTTGTTAATGCTGGAGAAACTACTCTGTCTTCAATGGATGTG ATCGATTACACAATCATCATCAATGGTCTCTGCAAGGAAGGATTTCTAGTAAAGGCCTTAAATTTGTGCAGTTTTGCCAA AAGCAGAGGTGTCACTCTCAATACCATAACATATAATTCACTCATAAATGGACTTTGCCAGCAAGGTTGTCTTGTAGAAG CTCTCCGTCTATTTGATTCGCTTGAGAATATTGGTTTAGTCCCATCTGAGGTCACCTACGGCATTTTGATTGACAATTTA TGCAAAGAAGGGTTGTTTCTTGATGCTGAGAAACTATTGGACAGTATGGTATCTAAGGGACTTGTGCCAAACATTATCAT TTATAATTCGATAGTTGACGGGTACTGCAAGCTGGGGCAAACAGAGGACGCCATGAGAGTTGTATCACGGAAAATGATGG GAAGGGTAACACCAGATGCATTCACGGTCAGTTCTATGATCAAAGGTTACTGTAAAAAAGGTGACATGGAAGAAGCTCTC AGTGTGTTTACAGAGTTCAAGGATAAAAATATCTCTGCCGATTTCTTTGGTTTTCTGTTTCTGATCAAAGGCTTCTGCAC GAAAGGGCGAATGGAAGAAGCAAGGGGCCTCTTGAGAGAAATGCTTGTTTCAGAATCTGTTGTTAAGCTGATTAACAGAG TTGATGCGGAATTAGCTGAATCTGAATCGATCAGAGGCTTCCTCGTCGAGCTGTGCGAACAAGGAAGGGTCCCTCAGGCT ATCAAAATCCTAGATGAGATAAGTTCAACAATCTATCCGTCTGGTAAAAACCTGGGTTCTTACCAAAGGTTGCAGTTCTT GAATGATGTGAATGAAGAAGAGATAAAGAAGAAAGACTATGTTCATGATTTTCATAGCCTGCACTCAACTGTTAGCTCAC TCTGCACCAGTGGGAAGCTGGAGCAAGCTAATGAGTTCGTTATGTCTGTGCTCTCTTGTATGCCTAGATAGGTCAGTATG TTTACTTGACTTCTCAAGTCACCAAAAGCCAACCGGAAATAATCAGAGTTATTCGAATTGGATTATCCATGTGGTTAGAC ATTTAATGGG ...............................i...N................................N........... ...........................................i.......................N....N....... ................................................................................ ...................................N............................................ ................................................................................ ..............................................................................N. ................................................................................ .........N..................N....................N.............................. ................................................................................ ..i......N...................i..N............N...........................N...... ................................................................................ .................................................................N.............. ..................N............................................N................ .............N.......i..............i..............N............................ ........N....................N...............................N.................. ...............................................N................................ ...............................N...........N..............N..................... ...................i...........i................................................ ...N..........i........................N..........................N............. ........................................N....................................... .......i...........i................................i.........N...........N..... ...........i........i...................................N....................... ........................i......N.................................N.............. .....N...................i...................................................... .......................................N......................................N. ......................N.............N......N..N...............N................. ......................N.....i................................................... ........................N....................N.........................i...N.... ......................N......................................................... ...............................................N................................ ...............................................................................N ......................N......................i.................................. ....................................................i....................i..i... ...............N...................N.............................i.............. ................................................................................ ..........i.............................i....................................... ...N............................................................................ .............N.................................................................. ..N..N.....i..........................N.....N................................... ..............................N..........N.................N.................N.. ....................................................................N........... .....N.... Pos Score Pred ------------------------ 32 0.691 Yes 36 0.106 - 69 0.301 - 124 0.518 Yes 148 0.302 - 153 0.359 - 276 0.128 - 479 0.464 - 570 0.404 - 589 0.376 - 610 0.236 - 723 0.835 Yes 730 0.118 - 750 0.736 Yes 753 0.359 - 766 0.449 - 794 0.426 - 946 0.237 - 979 0.500 - 1024 0.125 - 1054 0.150 - 1062 0.650 Yes 1077 0.651 Yes 1092 0.464 - 1129 0.241 - 1150 0.080 - 1182 0.396 - 1248 0.448 - 1312 0.247 - 1324 0.096 - 1339 0.213 - 1380 0.675 Yes 1392 0.615 Yes 1444 0.202 - 1455 0.611 Yes 1480 0.082 - 1507 0.131 - 1561 0.172 - 1608 0.654 Yes 1620 0.516 Yes 1653 0.629 Yes 1663 0.297 - 1675 0.149 - 1692 0.777 Yes 1701 0.705 Yes 1737 0.320 - 1785 0.680 Yes 1792 0.215 - 1826 0.169 - 1846 0.138 - 1866 0.575 Yes 1960 0.117 - 1999 0.164 - 2023 0.208 - 2037 0.167 - 2044 0.052 - 2047 0.070 - 2063 0.105 - 2103 0.194 - 2109 0.522 Yes 2185 0.115 - 2206 0.188 - 2232 0.691 Yes 2236 0.095 - 2263 0.336 - 2368 0.313 - 2480 0.245 - 2503 0.360 - 2526 0.716 Yes 2613 0.677 Yes 2634 0.690 Yes 2637 0.544 Yes 2656 0.198 - 2676 0.151 - 2706 0.754 Yes 2811 0.761 Yes 2841 0.774 Yes 2884 0.272 - 2974 0.261 - 3043 0.190 - 3046 0.368 - 3052 0.621 Yes 3079 0.148 - 3085 0.095 - 3151 0.303 - 3162 0.329 - 3180 0.301 - 3198 0.133 - 3269 0.042 - 3286 0.047 -

Explain the output -- Processing locus 30: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAAAATCAAATAAATTAGGATCAGAGAAGAAATTTATAGAACCGCCATTTCTACTCTCGCAAATAGCTAACCATCAAAC ACCAATTTCCATATCACGTAAGCTATATATATTTTTATTTTATACACTCCTTTCTCTCAACTTCTTTATTTTTATATACA ACTCTTAAAAGTAAGTATATTATATTTATTTAAGTGGTTTATGCTTTATATAAGAAAGAAAATGACAAGTATGATCGTGA AGACAACGCCGGAGCTCTTCAAAGGAAATGGAGTGTTCCGTACGGATCATCTCGGAGAAAACCGAATGGTTAGTCGATCA AACCGGCTAGGTGATGGATCAAACCGTTTCCCTAGAACCGGTACAATCCATTGCCAACGGTTAAGCATAGCAAAGACCGG TTTGCATCGTGAGACGAAGGCTCGAGCCATACTTAGCCCTGTGTCCGATCCGGCCGCTTCCATAGCCCAAAAGCGAGTAT TCACCTTTGGAAAAGGAAGAAGCGAAGGCAACAAGGGCATGAAGTCCTTGTTGGGAGGGAAAGGAGCCAACCTGGCGGAG ATGGCTAGCATAGGCTTGTCGGTGCCGCCGGGGCTAACCATATCGACGGAGGCTTGTCAGCAGTATCAGATCGCCGGCAA AAAGCTTCCAGAAGGTTTATGGGAAGAGATCTTAGAAGGTCTTAGCTTCATCGAACGTGACATTGGAGCTTCCCTCGCTG ATCCCTCCAAGCCACTCCTCCTCTCTGTTCGCTCCGGCGCCGCCATCTCAATGCCTGGTATGATGGACACTGTACTTAAC CTTGGCTTGAACGACCAAGTCGTCGTTGGTCTGGCCGCAAAAAGCGGAGAGCGTTTTGCTTACGATTCGTTCCGGCGTTT TCTTGATATGTTTGGTGATGTTGTGATGGGAATTCCACACGCCAAGTTTGAAGAGAAGTTAGAGAGAATGAAGGAGAGGA AAGGAGTTAAAAATGACACTGACTTAAGCGCGGCTGATCTCAAGGAATTGGTTGAGCAGTACAAGAGTGTTTACTTAGAG GCCAAGGGTCAAGAGTTTCCTTCAGATCCAAAGAAGCAATTGGAGCTAGCGATTGAAGCGGTATTCGATTCTTGGGATAG CCCGAGAGCGAACAAGTACAGAAGTATTAACCAGATAACTGGATTGAAAGGAACCGCGGTTAACATTCAGTGTATGGTGT TTGGAAACATGGGGGACACTTCAGGGACTGGTGTTCTCTTCACTAGGAACCCTAGCACAGGAGAGAAGAAGCTTTATGGC GAGTTTCTAGTTAATGCTCAGGGAGAGGATGTGGTTGCAGGGATAAGAACACCAGAAGATTTGGATACAATGAAGAGATT TATGCCTGAGGCTTACGCTGAACTTGTTGAGAACTGCAACATCTTAGAAAGACATTACAAAGACATGATGGATATTGAAT TCACAGTACAAGAAGAGAGATTGTGGATGCTGCAATGCAGAGCGGGTAAGCGAACGGGTAAAGGCGCCGTGAAGATAGCA GTTGATATGGTAGGTGAAGGGCTTGTTGAGAAATCTTCTGCTATCAAAATGGTGGAGCCTCAACATCTTGATCAACTACT TCACCCACAGTTTCATGATCCATCGGGGTATCGTGAAAAAGTGGTGGCCAAAGGCTTACCTGCGTCACCAGGAGCGGCGG TTGGACAGGTTGTGTTCACGGCGGAGGAAGCCGAAGCTTGGCATTCTCAGGGTAAAACTGTGATTCTGGTTCGAACTGAG ACAAGCCCTGACGATGTGGGAGGTATGCACGCAGCGGAAGGTATATTGACGGCTAGAGGAGGAATGACGTCACACGCGGC TGTTGTTGCTCGCGGTTGGGGAAAATGTTGCATTGCTGGTTGTTCCGAGATTCGTGTCGACGAGAACCACAAGGTTCTAT TGATTGGAGATTTGACGATTAATGAAGGCGAATGGATCTCAATGAACGGATCAACCGGTGAGGTTATATTAGGGAAACAA GCATTGGCTCCTCCGGCTTTAAGTCCAGATTTGGAGACTTTCATGTCCTGGGCTGATGCAATCAGACGTCTCAAGGTTAT GGCGAATGCGGATACACCTGAAGACGCCATTGCAGCTAGGAAAAACGGAGCTCAAGGAATCGGGCTTTGTAGGACAGAGC ATATGTTCTTTGGAGCAGATAGGATTAAAGCAGTGAGAAAGATGATAATGGCGGTAACAACAGAGCAAAGGAAAGCTTCT CTCGACATCTTGCTTCCTTACCAACGTTCGGATTTCGAAGGGATCTTCCGTGCTATGGATGGTTTACCGGTAACAATCCG TTTGTTAGACCCTCCGCTTCACGAGTTTCTCCCGGAAGGCGACTTGGACAACATTGTACATGAGCTAGCTGAAGAAACTG GTGTGAAAGAAGATGAAGTCTTGTCACGGATAGAGAAACTCTCTGAAGTGAATCCAATGCTTGGTTTCCGCGGTTGCAGG CTCGGAATATCGTATCCAGAGCTAACGGAGATGCAAGCGCGTGCAATTTTTGAAGCTGCAGCGTCAATGCAGGACCAAGG TGTTACTGTCATTCCTGAGATTATGGTTCCACTTGTAGGAACTCCTCAGGAATTGGGTCACCAAGTTGATGTAATTCGTA AAGTTGCAAAGAAAGTATTTGCTGAGAAGGGTCATACCGTGAGCTACAAGGTTGGGACAATGATTGAGATCCCTCGAGCC GCGCTCATTGCAGATGAGATTGCGAAAGAGGCGGAGTTTTTCTCGTTCGGGACAAACGACTTGACGCAGATGACGTTTGG ATACAGTAGAGACGATGTCGGCAAGTTTCTACCGATTTACCTCGCCAAAGGAATCTTACAGCACGACCCTTTTGAGGTTC TTGATCAGCAAGGTGTAGGGCAATTGATCAAGATGGCGACAGAAAAAGGACGAGCAGCTAGGCCTAGCCTCAAGGTTGGG ATATGTGGAGAACATGGAGGAGATCCATCTTCTGTGGGATTCTTTGCTGAAGCAGGACTTGACTATGTCTCTTGTTCTCC TTTCAGGGTTCCAATTGCAAGGCTTGCAGCTGCTCAAGTAGTTGTTGCATGATGAAGAAAAAACTTGGTGGTGTTATTCT GTGAATAAAAAAGCTTTGGTTTCTATTCCATAAAAATGAACAAAATCAAAGATATTGGATTTAACTATTTT ................................................................................ ................................................................................ ........................................N....................i........i......... ...........................i.....................................N.............. .............i.................................................................. ................................................................................ ......................................i......................................... i............................................................................... ..................N............................................................. ..................................................i........i..i................. ................................................................................ .......i.........N.......i.........................................i............ ............N................................................................... ................................................................................ .........................................................................N...... ........i..................................................................N.... .............N..............N........................................i.......... ..........................i.......N............................................. ......N.........................................i............................... ..............N................................................................. ................................................................................ .............N..........i......................................i................ ........................i....................................................... .....................N.........i.........i...................................... ..........................................i............N......................i. .....N.......................................................................... ..N......................................N.....i................................ ......................................................i...N..................... ...........................................................N.................... ............N...........................................N....................... ..............................i...................................i............. ......................i.............................................N........... ...........................................................i.................... .............N.......................................................N.......... ..............N................................................................. ................................i............................................... ..N..........N..................................................N............... ................................................N..N............................ ...................................N................................... Pos Score Pred ------------------------ 201 0.437 - 222 0.793 Yes 231 0.754 Yes 268 0.746 Yes 306 0.484 - 334 0.552 Yes 519 0.738 Yes 561 0.573 Yes 659 0.463 - 771 0.778 Yes 780 0.756 Yes 783 0.640 Yes 888 0.692 Yes 898 0.106 - 906 0.505 Yes 948 0.706 Yes 973 0.415 - 1194 0.213 - 1209 0.818 Yes 1276 0.258 - 1294 0.176 - 1309 0.264 - 1350 0.647 Yes 1362 0.478 - 1425 0.608 Yes 1428 0.776 Yes 1467 0.709 Yes 1475 0.403 - 1527 0.417 - 1569 0.601 Yes 1615 0.203 - 1774 0.264 - 1785 0.789 Yes 1824 0.775 Yes 1865 0.545 Yes 1942 0.196 - 1952 0.577 Yes 1962 0.659 Yes 2043 0.677 Yes 2056 0.354 - 2079 0.771 Yes 2086 0.216 - 2163 0.401 - 2202 0.361 - 2208 0.783 Yes 2295 0.664 Yes 2299 0.343 - 2380 0.369 - 2413 0.329 - 2457 0.358 - 2511 0.707 Yes 2547 0.663 Yes 2583 0.711 Yes 2629 0.062 - 2700 0.789 Yes 2734 0.139 - 2790 0.274 - 2815 0.317 - 2913 0.808 Yes 2963 0.202 - 2974 0.298 - 3025 0.253 - 3089 0.175 - 3092 0.151 - 3156 0.083 -

Explain the output -- Processing locus 31: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 32: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTGAAAAAAAAAAGAAACGATTTTTATTGGTTAAAGGGACAGACCTCCTTTTGGTGGATTGGCTCCGTGATCCAATCC TCGGAATTGTTTTTAAGGTGACGGAACAAATTTGTAAATCTTGTTTTTGGTATTGACCCAGATCTTTGGATAAATTTGAA ATTTTTATTGGTTGTTTTTGATTTGAAGATGCAGCATAATCTCTTCACAACAATGCGAAGTTTGAAGCTTGCTGAAGGTT GTAAAGGTACACAAGTTTACGCTCTTAACCCATCAGCTCCTACTCCGCCTCCTCCTCCTGGTAATAGCAGCACCGGTGGT GGTGGTGGTGGTGGTAGCGGCGGTGGCACCGGAGGTGTTGGGGATAAGCTTTTACAACATCTTTCAGATCATCTTCGTGT TAATTCTGTCCGATCTAAATCAAGCCGGACTTATCCTCCTCCTACTCAACCAAACGCTGTCGTTTCTCCCGAGTTTCTTC TCCCTTGTGGACTTCCTGTTACGGATCTGCTTGAACCTCAAATCGATCCTTGCTTGAAATTCGTTGATTTGGTTGAGAAG ATGGCTCAAGTTTATCGTAGGATTGAGAATTGTTCTCAGTTTGAGAAATCTGGGGCTTATTTAGAGCAATGCGCTATCTT CCGGGGCATTTCTGATCCGAAACTGTTCCGGCGGAGTCTCCGGTCGTCTAGGCAACATGCGGTGGATGTTCACGCGAAAG TCGTGTTAGCCTCGTGGCTTAGTTCGAGAGGAGGAGATGAGTTGATTGGAACGACTTCTATGGATTGTTGTGGCAGGAAT TTGGAATGTCCAAAGGCTACTCTTGTTTCTGGTTATGATCCTGAATCTGTTTATGATCCATGTGTTTGCTCTGGAGCTTC TAGATCAGAGATGATGAATGAAGATGAATGTTCTACTTCTCAAGAAGTAGACTATGATATGTCATTTTGTATTGGTGATG AAGAGGTTCGTTGTGTGAGGTACAAAATCGCGTCTTTGTCCAGACCTTTCAAGGCGATGTTATACGGTGGGTTTAGAGAA ATGAAACGAGCCACGATCAATTTTACGCAGAATGGAATCTCTGTTGAAGGGATGAGAGCTGCAGAGATTTTCAGCAGGAC CAACAGGTTAGATAACTTCCCTCCTAATGTTGTCTTGGAGCTTCTTAAGCTTGCAAATCGGTTCTGCTGCGATGAATTGA AATCCGCTTGTGATTCGCATTTGGCACATCTTGTTAATTCTCTGGATGAGGCAATGTTGTTGATTGAGTACGGATTAGAA GAGGCTGCGTATCTTCTGGTGGCAGCTTGTCTTCAGGTTTTTCTCAGGGAATTGCCTAGCTCTATGCATAATCCGAATGT TATAAAGATTTTCTGCAGCGCCGAGGGACGGGAAAGACTGGCGTCTCTTGGCCATGCTTCTTTTACATTGTACTTCTTCT TGAGCCAGATTGCGATGGAAGATGATATGAAATCTAACACAACTGTGATGCTCTTAGAACGTCTGGTTGAATGTGCGGTG GATAGTTGGGAGAAACAGCTTGCGTATCACCAATTAGGAGTGGTGATGCTAGAGAGAAAAGAATATAAGGATGCTCAGAG ATGGTTTAACGCAGCGGTTGAGGCTGGCCATCTTTACTCTCTCGTGGGTGTTGCCAGGACTAAGTTTAAGCGTGACCACA GGTACTCGGCTTATAAGATCATCAATTCCTTGATTTCGGATCACAAGGCTACTGGGTGGATGCACCAGGAAAGGTCCTTG TATTGCAGTGGTAAAGAAAAGCTGCTTGATTTGGATACAGCTACCGAGTTTGACCCTACTTTGACATTCCCCTACAAGTT CAGGGCAGTGGCTTTGGTGGAAGAAAACCAGTTTGGAGCTGCAATCGCAGAACTGAACAAGATTCTTGGATTCAAGGCCT CTCCTGACTGTCTAGAGATGAGGGCATGGATTTCTATAGGTATGGAGGATTACGAGGGTGCTTTGAAAGATATTCGGGCG CTTTTGACATTGGAGCCAAATTTTATGATGTTTAACTGGAAAATCCATGGTGATCATATGGTGGAGTTGCTCCGTCCGCT AGCCCAGCAGTGGAGCCAGGCTGATTGCTGGATGCAGCTGTATGATCGTTGGTCTTCTGTGGATGATATTGGATCTCTAG CAGTTGTTCATCATATGCTTGCAAATGATCCAGGGAAGAGTCTTTTACGGTTCAGACAGTCTCTTCTTCTCCTACGGCTC AATTGTCAAAAGGCAGCAATGCGTAGTCTAAGGCTGGCTCGAAACCATTCAAAGTCGGAGCATGAGAGACTTGTGTATGA AGGATGGATACTGTATGATACAGGCCACCGCGAGGAAGCCCTTGCCAAAGCGGAGGAATCCATTTCCATCCAAAGGTCGT TTGAAGCGTTTTTCCTCAAAGCTTACGCCCTTGCAGACTCTACGCTTGACCCGGACTCCTCTAATTATGTGATTCAGCTC CTTCAAGAAGCGCTTAAGTGCCCCTCAGATGGTCTCCGTAAGGGACAAGCATTGAACAACCTGGGGAGCGTATACGTGGA TTGTGAAAAGCTGGATCTTGCAGCTGATTGCTACACAAACGCGCTCACCATCAAGCATACACGAGCTCACCAAGGTCTTG CACGTGTTTATCACCTGAAAAACCAACGGAAAGCTGCTTATGATGAAATGACAAAGCTAATAGAGAAGGCTCAGAACAAT GCGTCGGCATATGAGAAGCGCTCTGAATATTGTGACCGCGAGATGGCTCAGAGCGACCTTTGCCTGGCAACACAACTTGA CCCTCTCCGAACCTATCCTTACAGATACAGAGCCGCAGTTCTTATGGACGACCACAAAGAAAGTGAAGCCATAGACGAGC TTTCGAGGGCGATATCATTTAAACCAGATCTTCAGCTATTACATCTACGAGCAGCATTCTATGATTCAATGGGAGAAGGA GCGTCTGCTATAAAAGATTGTGAAGCAGCATTATGTATTGATCCAGGTCATGCAGATACACTCGAGCTCTACCACAAAGC TCGTGAACCAAACGACCAAAAGTAGAACAAAACTATCAATTTGGTTTGATGGGCAAAAATATTTCTCTTCTTCCCTCCTG GTATGGCTTCTACACTGTTTCATTTTCCTTGTGCAAAACAGAAC ................................................................................ ................................................................................ ............................N.......................i........................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ i...................................................................i........... ........................................................N........N.............. ....................................N......................i.................... .....N............................N.................N......N.................... ..........i..i...N.....N...N.........................N....i..................N.. ........................................................N....................... i..............................N...................i............................ ..........................N............................................N........ .............................................N.......N.......................... ...............................................................N............N... .....................................................N.......................... ..............i......N....i....................N......................N......... .............................................N........................i......... N............................................................................... ...........................................................i.................... ................................................................................ ................................................................................ .................N.......N...............N...................................... ........................N..N..................N..........i...................... ...............................N.........N....................N................. ..............N.........N....................................................... ..................i..........................................N..............N... ...i..........N................................................................. ..................................................................i............. ............................i................................................... ................................................................................ .......................................N..N....i..............................N. ..........N...............................i..................................... ...........................................i.................................... ............................................................N.......i........... ................................N................N.............................. ................................................N............................... ..N......................................... Pos Score Pred ------------------------ 189 0.484 - 213 0.624 Yes 561 0.786 Yes 629 0.610 Yes 697 0.429 - 706 0.179 - 757 0.191 - 780 0.549 Yes 806 0.212 - 835 0.109 - 853 0.101 - 860 0.186 - 891 0.886 Yes 894 0.764 Yes 898 0.456 - 904 0.271 - 908 0.205 - 934 0.126 - 939 0.621 Yes 958 0.142 - 1017 0.235 - 1041 0.795 Yes 1072 0.235 - 1092 0.554 Yes 1147 0.144 - 1192 0.238 - 1246 0.085 - 1254 0.335 - 1344 0.292 - 1357 0.233 - 1414 0.196 - 1455 0.569 Yes 1462 0.285 - 1467 0.678 Yes 1488 0.456 - 1511 0.417 - 1566 0.295 - 1591 0.535 Yes 1601 0.464 - 1740 0.544 Yes 1938 0.437 - 1946 0.458 - 1962 0.412 - 2025 0.247 - 2028 0.455 - 2047 0.255 - 2058 0.537 Yes 2112 0.264 - 2122 0.170 - 2143 0.115 - 2175 0.469 - 2185 0.309 - 2259 0.721 Yes 2302 0.310 - 2317 0.391 - 2324 0.653 Yes 2335 0.247 - 2467 0.578 Yes 2509 0.589 Yes 2680 0.152 - 2683 0.241 - 2688 0.664 Yes 2719 0.444 - 2731 0.472 - 2763 0.610 Yes 2844 0.512 Yes 2941 0.286 - 2949 0.801 Yes 2993 0.243 - 3010 0.194 - 3089 0.170 - 3123 0.092 -

Explain the output -- Processing locus 33: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTCTCATGACTAAATCAAACCATTTTTTATTTTTTTCTAGTCAACACTAATCTTTATTATTTTATCACTCTTTTCTTGA TGAGTACCAATTTTGTTATAGCATTGTAAAGTCTTCCAAGACCTTTTCAAACCTATTTATTTCCTCTGTATTTATAACAG AAAAAGGGAAGTTTTAGAGAACCACATAACTTGTCTGTCTCCTTATCTTTGGATAATGATGAACCCTAAAAAAAACAATA ACACCTTTCTGAGCTACTTTGTTTGTTTGTTTTTGTTGCTCGAGGTTGGTTTAGGACAAAACCAAATAAGTGAAATCAAA GTAGGAGTAGTTCTTGATCTCAATACAACATTTTCGAAGATCTGTCTCACTTCTATTAACCTGGCGTTGTCTGATTTCTA TAAAGACCATCCTAATTACCGCACAAGACTTGCGCTTCACGTTAGAGATTCCATGAAAGACACTGTGCAGGCTTCAGCTG CAGCCTTGGACCTAATCCAGAACGAGCAAGTGAGTGCCATCATCGGACCAATAGACTCTATGCAGGCAAAGTTTATGATC AAACTGGCCAACAAAACTCAAGTACCGACCATCTCATTCTCAGCAACAAGCCCTCTTTTAACATCCATCAAAAGCGATTA CTTCGTCCGAGGCACTATCGATGACTCATACCAGGTCAAAGCCATTGCAGCCATATTTGAATCCTTTGGATGGAGAAGTG TCGTCGCCATTTATGTGGACAACGAGTTAGGTGAAGGAATCATGCCTTACTTGTTTGACGCTTTACAAGACGTGCAAGTT GATAGAAGTGTGATCCCCTCGGAGGCTAACGATGACCAGATTCTAAAAGAACTTTATAAGCTCATGACGAGGCAGACTAG GGTTTTCGTTGTCCACATGGCATCACGTCTTGCTTCACGGATTTTTGAGAAAGCTACAGAGATTGGGATGATGGAGGAAG GGTATGTATGGTTAATGACCAATGGAATGACTCATATGATGAGACATATCCATCATGGTCGTAGCTTAAATACTATAGAT GGCGTGTTAGGTGTGAGGAGCCATGTCCCCAAATCGAAAGGGCTCGAAGATTTCCGTTTGAGATGGAAAAGAAATTTCAA GAAGGAGAATCCATGGCTGAGGGATGATCTTAGCATTTTTGGATTATGGGCGTATGATTCAACCACTGCATTGGCCATGG CCGTGGAGAAAACCAACATAAGCAGCTTTCCGTATAATAATGCGAGTGGCTCGTCAAATAACATGACAGATTTGGGGACG CTACACGTCTCTCGCTATGGTCCAAGTCTTCTAGAGGCTCTCTCGGAAATAAGATTCAATGGTCTGGCAGGAAGGTTTAA TCTCATAGACAGGCAGCTCGAGTCACCAAAGTTTGAGATCATAAATTTTGTCGGAAATGAAGAGAGAATTGTCGGATTTT GGACACCGAGCAATGGACTTGTGAATGTAAATTCAAACAAAACAACGTCATTTACAGGGGAGAGGTTTGGGCCATTGATA TGGCCAGGGAAGTCTACTATTGTTCCTAAAGGATGGGAGATTCCAACTAATGGTAAGAAGATTAAAGTGGGTGTTCCAGT AAAGAAAGGCTTCTTCAACTTTGTGGAGGTAATCACGGATCCTATCACTAACATAACAACTCCAAAGGGTTACGCCATAG ACATCTTTGAAGCTGCTCTTAAGAAGTTACCATATTCAGTCATTCCACAATACTACCGTTTCGAGTCTCCGGATGATGAC TACGATGACTTGGTCTATAAAGTCGATAACGGGACTTTGGATGCAGTTGTTGGAGATGTAACCATCACAGCGTACAGATC TTTGTATGCTGACTTTACCTTACCATACACAGAGTCAGGAGTGTCTATGATGGTGCCGGTGAGAGACAACGAGAATAAGA ACACATGGGTGTTCCTCAAACCTTGGGGCTTAGACTTATGGGTCACCACTGCTTGTTTCTTCGTCCTCATTGGTTTTGTT GTGTGGTTGTTCGAACATAGAGTCAACACCGACTTCCGTGGACCGCCTCACCACCAGATCGGCACTAGTTTTTGGTTCTC TTTCTCCACCATGGTTTTTGCCCACCGTGAGAAGGTAGTGAGCAACTTAGCAAGGTTTGTGGTGGTTGTATGGTGCTTTG TGGTGCTTGTGCTCACTCAGAGCTACACAGCAAATCTCACCTCTTTCTTGACGGTGCAACGTTTTCAACCAGCGGCCATA AATGTGAAAGATCTCATAAAAAATGGGGACTATGTAGGCTATCAACACGGGGCTTTTGTTAAAGATTTTCTAATAAAAGA GGGATTTAATGTTTCTAAGCTCAAGCCTTTTGGGTCTTCCGAAGAATGTCACGCGCTTTTGTCCAATGGTAGTATATCAG CAGCTTTCGACGAAGTTGCTTACCTTAGGGCTATCCTTTCTCAATACTGCTCCAAATATGCCATTGTTGAGCCTACATTT AAGACTGCTGGTTTTGGCTTTGCATTCCCCAGGAATTCACCTTTGACAGGAGATGTCTCAAAGGCTATCTTAAATGTGAC TCAAGGAGATGAAATGCAACACATCGAGAACAAATGGTTCATGAAACAAAATGATTGTCCTGATCCAAAGACCGCTCTTT CGTCGAACCGTCTAAGCCTTCGTAGCTTTTGGGGTCTGTTTCTAATAGCAGGCATTGCTTCATTCTTGGCACTACTCATC TTCGTGTTTCTTTTCTTGTACGAAAATAGGCATACACTATGTGATGATTCCGAAGATTCGATATGGCGTAAGCTAACGTC TTTATTCAGAAACTTCGATGAGAAAGACATAAAGTCGCATACTTTCAAGAGCAGCGCTGTTCATCATGTGAGTTCACCTA TGACTCAGTACATTCCGAGCCCTTCAACTTTGCAAATCGCACCACGGCCACATAGCCCATCACAAGACAGGGCGTTTGAA CTAAGAAGAGTGTCATTTACCCCGAATGAAGAACGGTTAACTACGCAAACTATACATTTTGAAGATGAAGAATCTGATAT TGAATGTGTTGTTGAACAATAGAGATAAGTTTAGAAGATTCCATAACAAGTTATAGATAGGATAAGCTAAGTTCGCTGCA TATATTCCTAACAAAGCTATTATTCTTTTATTTATAACAAA ......N........................................................................N ................................................................................ .......................................................N..i..................... ................................................................................ ................................................................................ ....................................................N........................... ...........................................................N..............N..... ................................................................................ ....................N................................................N.......... ............N............................i...................................... ...............................N...............................i................ ................N..................................................N..N......... ...N...i......i......N....i........N..N...............N.......................i. ......................N.......................................N................. ............i..........N.....................N.......N......................N... .......................................i......................i................. ................N.........................................N..................... ........................................................N....................... ............N...........N......................................................i ................................N................N.............................. ................................................................................ ........................................................................N..N.... ....N...................................N..............N........................ .....N........................................N..i.............................. ....i................................N.......................................... ................................................................................ ..........i..........................................................i.......... ................................................................................ ........N....................................N...................N.............. .........................................................N...................... ....................................................N....................N...... ........N....N...................i......N.........N............................. ................................................................................ ......................................N....N..................i................. .................N...............................................i.............i ................................................................................ .........................N......................................N............... ...N............................................................................ ......................................... Pos Score Pred ------------------------ 7 0.206 - 80 0.252 - 216 0.411 - 219 0.542 Yes 453 0.364 - 540 0.275 - 555 0.319 - 661 0.447 - 710 0.372 - 733 0.238 - 762 0.666 Yes 832 0.248 - 864 0.669 Yes 897 0.365 - 948 0.384 - 951 0.484 - 964 0.269 - 968 0.647 Yes 975 0.503 Yes 982 0.383 - 987 0.777 Yes 996 0.249 - 999 0.281 - 1015 0.089 - 1039 0.591 Yes 1063 0.129 - 1103 0.346 - 1133 0.786 Yes 1144 0.214 - 1166 0.414 - 1174 0.184 - 1197 0.490 - 1240 0.570 Yes 1263 0.647 Yes 1297 0.284 - 1339 0.350 - 1417 0.262 - 1453 0.412 - 1465 0.138 - 1520 0.533 Yes 1553 0.457 - 1570 0.129 - 1753 0.144 - 1756 0.276 - 1765 0.309 - 1801 0.180 - 1816 0.364 - 1846 0.131 - 1887 0.269 - 1890 0.677 Yes 1925 0.648 Yes 1958 0.308 - 2091 0.540 Yes 2150 0.761 Yes 2242 0.572 Yes 2263 0.475 - 2272 0.670 Yes 2329 0.224 - 2366 0.212 - 2386 0.231 - 2458 0.221 - 2533 0.372 - 2554 0.212 - 2569 0.339 - 2574 0.499 - 2594 0.533 Yes 2601 0.462 - 2611 0.436 - 2759 0.456 - 2764 0.337 - 2783 0.591 Yes 2818 0.226 - 2866 0.590 Yes 2880 0.606 Yes 2986 0.171 - 3025 0.270 - 3044 0.199 -

Explain the output -- Processing locus 34: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCAGATAGAAACAAAAGATTGGATATTTCAGACACAATCGATCAAAGAATAAACTATTCGTCGCTCTCTTCTCAATCAG CCGTAAAAGTAAGTACTTTGATTCCTGAATCAGATGTTGTTACTTCATTTTTTTCAATTTGGACCCTTATGCATAAATTT CAATTCTTTGCATCGCTTGTTACAATTTTTCACATTGGTTTTTCTTTTTCTTCTCTGTGGACGACTCAGAATGGCAGCTG ATCAGAGGAGAAAGCGGATGAACAGTGCCAATGTTATTGGTACCAGTTCTCGGGAGCATTACAGAGCAAAAAGGAAAAAG AATGCTTCACCAGATGGTGCTTTGCGTTCCGGTGATCACATCACTCTTGAATGGGATCGCAACCGAAGTAAAGTAGTCTC TAAGAAGGAACAAGTTGGTCTAAGTTTTAGGCATCTTCGTGAATTTGTTGATGTCGTTCCTCCTAGGCGTAATGTCTTGG CACAAGTATGCCCTGTCCCGCATGAAACTTTCCAGTTGGAAAATCTGTCGGAGGTGCTTTCTAATGAGGTTTGGCGATCT TGTCTGTCTGATGGTGAAAGAAATTATCTGCGGCAATTTCTCCCTGAGGGAGTTGATGTGGAGCAAGTTGTACAAGCATT GCTTGATGGCGAGAATTTTCATTTTGGAAATCCTTCTCTTGATTGGGGAACAGCTGTCTGCTCCGGTAAAGCTCATCCAG ATCAAATCGTTTCACGGGAGGAATGCCTGAGGGCTGATAAAAGGAGATATTACTCGAATTTAGAGAAATATCATCAAGAT ATTATAGATTATTTGCAGACATTGAAGGAGAAATGGGAAAGCTGCAAAGATCCAGAGAAGGATATTGTCAAAATGATGTG GGGGAGATCAAGAGGGGGCAATGCACAAGTGAACGGTAGCTGCCAGGGTCTGACTGCTGCCTCGGGGTCGAGTTCCTGGA ATGAAGATGATAAACCAGACAGTAGTGATAATATGATTTCTCCTGTTGTGAGGTGTGGAGAGGTTCAGAGAAGGTCCAAG CGCTCTGGTTTAGAGAAAGAAAAAACTCAAAATAATGGTGTAAACGTGGGTGGAAAAGTTAGAAAGAAGAACGTGTTGCC GAAAGATAGCATTCAGCAAACTGATGGTGCTAAATACATGTCCTATCTTAAGATAAGCAAGAAGCAGCATCAAATTGTCA CAAGCATGAAGCAGTCTGGTAAAAGCATTCAGTCGAGAGCACTTAATCGAATCTTCGGTAACATCGATAGTTTAGATGTT CAACCATACGGAGTTTTTGTGGAAGAAGAACAGAAGAAACTGAACGCTCACTGGCTGCATCTTGTTAAAGATCTTCCTGC AGCATATGCAATATGGAAAAGGCTACAGTTACAGAAACGAGATATAATCAGCTCTATGGGAAGGGAACTGAAGGAAAAAT GCAATCTGTGGATGGAGGATAAACAACAACAGTATGCTGCAGAAAACCCTCTGCAGAAGCATGATGTTCAATACAATAAT GGAGAAAGTTTGAATCCTAATCAGAGTGGTGATATGGCACCAGATGGTGAAGATTCTGGAATTTTCAGTCAAATTTCTGG CAAGAATCACTCCCCTTCAAAGGATTCTTCGTCTTATGGTGACCAAATCACAGATTCAGGTCGCTGCTTGCTAGTTGGTA CACACTCCTCACAGGTTTCATCACCAGATTGTGGAAATAACATCAATCACGAGGATAGAGAAGAGAAACAATATTCTTCT CCTTGTTCTCCCAGCAGGTGTCACAGCTTTAACCGAACAGATGTTGAAGTAAATAATTATTCCAGCTCTATACAAGGTCA GTCTCTCCCACAGGCTTCATTTCCTAATGAGCCCCATGCTTCAGATCTCGAAGATGCAGATCATGTAGGCAAGCACTGTG CACCAGAACTAGAGAATGCTTCGTTAGACGAGAGGATTCCGTGTATTGCTACAAGCCTTGGAGAAGGGTCGCAGTTTTGT TCTGGTGGAGATGTGTGGCAGCCAGTGGGAGGGATTAGGCAGTCCTACATTAGCCGTCAAGCTTACACCCCCAGTGGTGG ATTGTCAATCATACACCATCCTGAAGGTGATGAAGTAGCTAAAAACTGTTTCATAGACCTGGAATCGAACATGCCTGAAG AAGTTGATAGGAGGAAGATGTTGCAGCGAAATGCAAACAACAGTTTTGGTTCTTTCCCTAACAACAACCAAAATGAAATA CTACAGTCTTTGTTTAATGGCCAGGGTATGGTATCTCGCACCACTGAGCAGCTTCATCCTCTTCTCAAAGTCCCACTTAA CGAGGAGCATAAACAAATTATGGGCATTGGTTTTCAGCAGGAAGGAAACAACAATATGATGGTGGGCAATCAATTTTCTG GTCAGTTCCAGGATCAGATTCCTGCATCTCATGCATTTCCCCAGGACCAGCAGAGACAGGTTGACGCCTTTGGCCAAGGA AGCATGTCAGACAACATTTACCGTGGATTCTTGATGCAGCGGCCAGACTGGAACCCCAATTGTACTCAGATAGGAGTTAC CGCACAGCCCCTGTTGAGCACCGGGCCTTTGTTAAATCAGAATTGGCAATTTAGGAGCATGTGGGCAAACACAAACGGTG TCGGATGTACAAGCCAAGGCAGTCAAACCGGGACCGAGAGAGATCCGAGCCTTCTCAGAGTTGCTAATAACGCAGAGCAA ATAATTCATAGGGGAAGTAGTTCAGATCAAAGCTTATTCTCTGTTCTCTCTCAATGCAGTCAACTGCGCCCATCCAGATC TGCTTTCGAGCCAGAAAGTTCAAGTCATCAAATGGTTGCGTCTGGTAACTACGGAATGCTGATGGGGGGAGGTTCAACTC AAGTAAGCAGCAATTTGGTTCAGCCTGCGAACCCGCTTGATTACTTGAGCGGTAGCAACCCTACAACATCTTTGATGCCA GATGATGTAGTGTGGATGAACCAGAGCCGCCAGAACTCTGGTCTCCATGACCCACTAGGCAAGTTGTATCCAAGGTCATG GAATCCGTAACCATGTTTCTAAAGTTTTGTGTTGCAGAAGCATCTGGTCATCTTTAAGATATACAAAACCCAACATGGGT TTCTTAGTTTTCATTTCTGTGCCTGTACA ................................................................................ .................................N..................................N........... ......................................................................i......... .................i............N................................................. ..................................................N....................N........ .......N.............N.........................................N................ ..........N............................................N........................ .....N.......................................................................... ......................i......................................................... ................................i.......................................i..i.... ....................N........................................................... N.....N.........................i............................................... ..................................N............................................. .......................N.............N.......................................... .....N.....................................................................N.... ................................................................................ .....N......i..........................................i......................N. ...........i.....................N..........................N..N..............i. .................................i.........N.................................... ...................................N............................................ ................................................................................ ........................................i....................................... ..........................N........N.................N........N................. ...............N................................................................ ..........N..................................................................... .............................N........................................i......... .................i............N.........................................N....... ................N..........i.................................................... ...................i...................................i..i..................... ..............................N................................................. ...i.............................N.............................................. ..........................................................i..................... ....N........................................................................... .....................................................N.......................... ...............................i.......................i.....N.................. ..........................................................................i..... ............N.............................................................N..... ............................. Pos Score Pred ------------------------ 114 0.045 - 149 0.365 - 231 0.872 Yes 258 0.751 Yes 271 0.449 - 322 0.456 - 334 0.202 - 371 0.363 - 451 0.141 - 472 0.232 - 488 0.479 - 502 0.204 - 544 0.332 - 571 0.259 - 616 0.159 - 646 0.496 - 743 0.589 Yes 833 0.525 Yes 873 0.803 Yes 876 0.701 Yes 901 0.161 - 961 0.268 - 967 0.138 - 993 0.736 Yes 1075 0.136 - 1144 0.068 - 1158 0.325 - 1206 0.495 - 1276 0.125 - 1366 0.252 - 1373 0.613 Yes 1416 0.518 Yes 1439 0.484 - 1452 0.623 Yes 1474 0.154 - 1501 0.206 - 1504 0.136 - 1519 0.510 Yes 1554 0.623 Yes 1564 0.259 - 1636 0.374 - 1801 0.564 Yes 1867 0.290 - 1876 0.313 - 1894 0.181 - 1903 0.146 - 1936 0.417 - 2011 0.430 - 2110 0.229 - 2151 0.522 Yes 2178 0.519 Yes 2191 0.300 - 2233 0.236 - 2257 0.241 - 2268 0.703 Yes 2340 0.542 Yes 2376 0.804 Yes 2379 0.831 Yes 2431 0.258 - 2484 0.721 Yes 2514 0.291 - 2619 0.715 Yes 2645 0.242 - 2774 0.474 - 2832 0.823 Yes 2856 0.594 Yes 2862 0.379 - 2955 0.542 Yes 2962 0.462 - 2965 0.455 - 2976 0.123 - 3007 0.153 - 3038 0.429 - 3053 0.122 - 3115 0.111 -

Explain the output -- Processing locus 35: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 36: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGATCGCCGAACAGATTAGAAACTTGTAGTTGATTCGAAATTAGACTTCTTTGTTGTTTCTGATTACCGATTTAGGTT TTTGTGTGTTTGTAAGTCTAAAGTTTGATGCTTTATGATTTTCTTGATCAGATGGAAGATAGAGAGACACATTTAGGAAC TCGGGAGGTTAATGAAACATCTCCTGATTTGTTAAAGAACACGCCTTCGAACATTGCGAGGTTGGAAGATGTGATTGAGC AATGCCATGGTAGACAAAAGTATCTTGCTCAGACTAGAAGTCCATCTGATGGGAGTGATGTTCGGTGGTACTTTTGTAAG GTTCCTTTGGCTGAAAATGAGTTAGCTGCTTCAGTACCTCGCACTGACGTAGTAGGAAAGAGTGAGTATTTCCGTTTTGG TATGAGGGATTCTCTTGCGATTGAGGCGTCTTTCTTGCAGAGAGAAGATGAGTTGTTGTCACTCTGGTGGAAAGAGTACG CAGAATGCAGCGAAGGTCCGAAACTGCAAGTTAATTCTAAAAAGAAATCAATTGAAACTCCTTCAGAAGCTTCCGTGTCC TCTAGTCTATATGAAGTTGAAGAGGAGCGAGTAGGTGTACCTGTTAAGGGTGGGCTATATGAGGTGGACTTGGTGAGGAG GCATTGTTTTCCCGTGTACTGGAATGGAGATAATCGGCGTGTGCTTAGAGGTCACTGGTTTGCTCGTAAAGGTGGCCTAG ACTGGCTTCCGATTCCAGAAACTGTTTCTGAGCAGTTGGAGGTTGCATATCGTAACAAGGTTTGGCGCAGAAGAAGTTTT CAACCCTCTGGGCTTTTTGCAGCTCGTATTGATTTGCAGGGTTCTAGTCTGGGACTCCATGCTCTCTTTACTGGGGAGGA TGACACCTGGGAAGCGTGGCTTAATGTTGATCCTTCTGGGTTCTCTGGCATCGTTGGATATACTGGGAATGGAATTAAGT TGAGACGTGGTTATGCTGGCTCCTACTCTCCAAAACCTACGCAGGAAGAACTTCGCCAGCAAAAGGAGGAAGAGATGGAT GACTATTGTTCTCAAGTCCCTGTTCGGCATCTTGTATTTATGGTTCATGGTATTGGCCAAAAAGGAGAAAAGTCTAATCT TGTTGACGATGTTGGAAACTTTCGTCAAATCACGGCAGCTTTAGCGGAACGTCATCTAACCTCTCATCAGCTCAGCACTC AACGAGTTCTTTTCATCCCATGCCAGTGGAGAAAGGGTCTAAAGCTAAGTGGTGAAGCTGCTGTTGATAAATGTACTTTA GACGGTGTACGGCGTTTCCGAGAGATGCTAAGCGCAACTGTTCATGATGTGTTATACTACATGAGCCCCATTTATTGTCA GGCTATAATTGATTCGGTTTCAAAGCAACTGAATAGACTGTATCTAAAATTTTTAAAGCGGAATCCAGACTATGTCGGAA AGATTTCCATTTATGGACATTCTCTGGGGAGTGTTCTCTCCTATGACATCTTATGTCATCAGCACAACTTGTCATCCCCA TTTCCAATGGATTCAGTATACAAGAAATTTTTCCCAGATGAAGAATCTCCTCCAACTCCAGCTAAAGCTGACAAACCTTG TAGTTCACATCCATCATCAAATTTTGAGCCAGAGAAATCTGACCAGTTGAATAACCCCGAGAAAATCACAGGTCAAGATA ATAACACGATGGCTAAAGAACCAACAGTATTGGAGCATCACGATGTCATCCAGGAAGATCCTTCATTGATCTCTGATTCT GTTGTGGCCAACGTGGGTTTGGAAAGACGAGGTGGCCAGGAAGATGACCATCATGATTCTAGTGGTGCTATATCCTCACA GGATGTTCCAGATGGAGCGGACTGTAGAACGCCTGATTCTCCATCATGTTCTCAAGAACAATCATGGGATAAAGAAAGTG TCAACTCTAATAATGAGGAGAGAATCAAGTTGTTACAAGATGAGGTTAACTCATTGAGGTCAAAAGTCGCACAACTGCTA TCAGAGAATGCCAGAATATTATCGGATGAAAAAGCCAAGACATCTGTGGCGCCTAAGGAGCTCAACAACGAGAAGGTGCA AACTGAAGATGCCGATGCACCTACAAGCTTTACTCCTTTTATCAAGTACCAAAAGCTTGAGTTCAAGGTTGACACTTTCT TTGCAGTTGGGTCTCCTCTTGGAGTTTTTCTTGCCCTTCGAAATATACGTCTTGGGATAGGTAAGGGAAAAGATTACTGG GAAGAGGAGAATGCTATTGAAGAGATGCCAGCTTGTCGTCGAATGTTCAATATATTTCACCCCTATGATCCTGTTGCATA TAGAGTGGAGCCACTTGTCTGCAAAGAGTACCTTCCCGAACGACCCGTTATTATTCCCTACCACAGAGGAGGCAAGCGGT TGCATATTGGATTACAGGATTTCAGAGAAGATTTTGCTGCACGTTCACAGAGAATAATGAATCATTTTGATTCAGTAAGG ACAAGAGTTCTCACTATTTGTCAATCCAAAAGTGCAGATAACCTAGACGAGATGGAAGAAACTGATGACGAAAAGGATGA TAGGTCCTATGGTTCCTTAATGATAGAGAGATTAACTGGAACTCGAGACGGTCGGATAGATCACATGCTCCAGGAGAAAA CCTTTGAGCATCCGTATCTACAAGCAATAGGAGCTCATACGAACTATTGGAGAGATCAAGATACTGCTCTTTTCATAATT AAACACTTGTATCGCGAGTTACCAGACGGACCAAACTCGCCCACGGAATCCACCGAAGGAGATGATAGTCCAAAAGACTC AAGTAGACCTCATAGCTGGATAGACAGAAGAGAGGCTGATTATGATGATGAAGAGCTTCCTTTAACATTCTCCGACAAAC AAATCACTAGAAGCTTCTCTGCAGAAGCCAAGAAATATTTGAAGAAGCCTTAAGACTTTGACCGTAGGTTTATATCGTAT ATCCGGTGAAAGCTCCCTGATTTTGTTTTTGTTTCCTGTGCAGAAGTAACATCTAGTGTAGAAAATGTGAAA ................................................................................ ...........................N......N................i............................ ...........N........................................................N........... ................N............................................................... ....N........................................................................... ..........N...............................................N..................... .......................N........................................................ ................................................................................ ..........................................................N....................N .......................N............................................N........... ............N.............................................................i...N. .......................................i......N................................. ........N....................................................................... ...................N..................................................N......... ........................N..................N..N.............i................... .......................................................................i........ ............N.............................N.........N........................... ......i..............................i.......................................... ................................................................................ ........i.................................N..................................... ...........................................N........N........................... ..N........N.................................N.................N................ ............N..........................N........................................ .......N.................N...................................................... ........N.....N................................................................. ................................................................................ ..........N.............i.................N.....................N............... ................................................................................ ........................................................i....................... ...................................................i............N...........N... ........N..........N............................................i............... ................................................................................ .............................................................i.................. .........................................N..N..N................................ ................................................................................ ................................................................N....... Pos Score Pred ------------------------ 108 0.476 - 115 0.152 - 132 0.616 Yes 172 0.137 - 229 0.489 - 242 0.315 - 247 0.504 Yes 289 0.210 - 298 0.200 - 337 0.178 - 402 0.357 - 448 0.312 - 485 0.213 - 571 0.252 - 619 0.189 - 664 0.152 - 859 0.210 - 880 0.184 - 904 0.325 - 949 0.326 - 973 0.167 - 1035 0.843 Yes 1039 0.321 - 1080 0.551 Yes 1087 0.087 - 1129 0.195 - 1220 0.296 - 1271 0.357 - 1305 0.465 - 1324 0.220 - 1327 0.414 - 1341 0.604 Yes 1432 0.607 Yes 1453 0.244 - 1483 0.219 - 1493 0.399 - 1527 0.659 Yes 1558 0.624 Yes 1689 0.796 Yes 1723 0.319 - 1804 0.275 - 1813 0.480 - 1843 0.212 - 1852 0.265 - 1886 0.414 - 1904 0.437 - 1933 0.388 - 1960 0.401 - 2008 0.308 - 2026 0.179 - 2089 0.140 - 2095 0.120 - 2251 0.372 - 2265 0.771 Yes 2283 0.398 - 2305 0.109 - 2457 0.742 Yes 2532 0.680 Yes 2545 0.159 - 2557 0.264 - 2569 0.176 - 2580 0.320 - 2625 0.540 Yes 2782 0.541 Yes 2842 0.457 - 2845 0.397 - 2848 0.293 - 3025 0.049 -

Explain the output -- Processing locus 37: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTTGAACTCTCCGACGAAATAATCGTCTTCTCCGGCATCATCTTCAGAAAGCTATTCCAAATTAGGGTTTTGACTTTT GATTGAAGAAGACAGGTCTAGAAACTTACATACACCAATTTTAAAATCGAGTTTGGGCCGAATTATGGACCGTACTTTGG GCTATGGGCCTTCATTTTAATAAACAGGTCGGATATATCCACCGGACCCGGAATGATCGTCTTCCTCAGTGTTGTATTTT GGCTTTCCTCATTGCTTCCTCAATCTAAGGATTTCCATGAACAAGGAACTAAAATGAGATCTCTTCTCTTTGTACTATCA CTCATTTGCTTTTGCTCTCAAACAGCACTTTCATGGAAGAAGGAAGAGTTTCGCAGCTGTGACCAAACTCCATTTTGTAA ACGCGCTCGATCTCGTACTCCCGGCGCGTGTTCTCTAATTGTCGGCGATGTTTCCATCACTGATGGAGATCTCGTAGCGA AGCTTCTACCGAAAGCGCCTAATCAAGGCGATGGGGATCAGATCAAGCCGTTGATTCTTTCTCTCTCAGTTTACAAGGAT GGGATCGTGCGGCTTAAAATCGATGAGGACCATTCGTTGAACCCGCCGAAGAAGAGGTTCCAAGTTCCTGATGTGGTAGT GTCTGAGTTTGAGGAGAAGAAGATCTGGCTGCAGAAAGTAGCGACGGAGACGATCTCTGGAGACACTAGTCCGTCTTCAG TAGTTTATGTATCCGATGGTTACGAGGCGGTGGTGCGACACGATCCGTTTGAGGTGTATGTGCGTGAGAAATCAGGTGAT CGCCGTCGCGTTGTGTCATTGAATTCTCATGGATTATTTGATTTTGAGCAGTTGGGGAGGAAAACTGAAGGAGATAACTG GGAAGAGAAATTTAGGACTCATACAGATTCTAGACCATCTGGTCCTCAATCTATTAGTTTCGATGTTTCGTTTTATGATT CCAGTTTCGTTTATGGAATTCCTGAACACGCCACTAGCTTCGCGTTGAAGCCTACCAAGGGTCCTGGAGTTGAGGAATCT GAACCCTACAGGCTTTTTAATCTAGATGTGTTTGAATACGATCATGAATCACCGTTTGGGCTTTACGGGTCGATTCCGTT CATGGTTTCGCATGGGAAGTCTGGTAAAACTTCAGGATTTTTCTGGTTGAATGCTGCGGAAATGCAGATTGATGTGTTGG CTAATGGTTGGGATGCAGAGAGTGGTATTTCTTTGCCTTCTAGTCACAGTAGGATCGACACATTCTGGATGAGCGAGGCA GGGATTGTGGATACATTCTTTTTCGTTGGGCCTGAGCCAAAGGATGTTGTAAAGCAGTATGCAAGTGTGACAGGTACTTC AGCCATGCCTCAGTTGTTTGCCACTGGTTATCATCAATGTAGGTGGAACTACAAAGATGAGGAGGATGTGGCACAGGTGG ACTCGAAATTCGATGAACACGATATTCCTTATGATGTTCTCTGGCTTGACATTGAGCATACAGATGGGAAGAGATACTTT ACATGGGATAGTGTGTTGTTTCCTCATCCAGAGGAGATGCAAAAGAAATTGGCTGCAAAGGGTAGGAAGATGGTGACCAT TGTGGATCCTCATATCAAGAGGGATGACTCATACTTCTTACACAAAGAGGCTACTCAGATGGGATACTATGTTAAGGATT CATCTGGAAAAGACTTTGATGGTTGGTGCTGGCCTGGTTCATCATCTTACATTGATATGTTGAGCCCAGAGATTAGAAAA TGGTGGGGTGGGAGGTTCTCGTATAAGAACTATGTTGGTTCAACTCCATCATTGTACACCTGGAATGACATGAATGAGCC TTCTGTATTCAATGGTCCCGAGGTTACTATGCCAAGAGATGCATTACATGTTGGGGGTGTTGAACACAGAGAAGTTCATA ACGCATATGGATATTACTTCCACATGGCGACTTCCGATGGACTTGTTATGCGTGAAGAAGGAAAGGATAGGCCTTTTGTA TTGTCAAGAGCAATCTTTCCCGGCACTCAAAGATACGGAGCAATTTGGACTGGAGATAACACAGCCGAATGGGAACACCT TAGAGTCTCCATTCCAATGATATTGACACTTGGTCTTACTGGAATTACATTCTCTGGAGCTGATATTGGTGGGTTTTTTG GAAATCCTGAACCAGAACTTCTAGTTAGGTGGTACCAAGTGGGTGCTTACTATCCATTTTTCAGGGGTCATGCTCATCAC GATACCAAAAGACGAGAGCCTTGGTTGTTTGGTGAACGGAACACAGAACTCATGAGAGATGCCATACACACTCGTTACAC ACTGCTCCCATACTTCTACACGTTGTTCAGAGAAGCAAACGTTACGGGTGTTCCTGTTGTACGCCCATTATGGATGGAAT TCCCGCAAGATGAAGCTACTTTTAGCAACGATGAAGCCTTCATGGTCGGTAGTGGTCTACTGGTTCAAGGAGTTTACACC AAGGGAACAACGCAAGCTTCCGTGTATTTGCCTGGCAAAGAATCATGGTATGACTTGAGAAACGGTAAGACTTACGTTGG AGGCAAGACTCACAAGATGGATGCTCCAGAGGAGAGTATTCCTGCGTTTCAAAAGGCAGGAACCATCATCCCAAGGAAGG ACCGGTTTAGGCGAAGTTCCTCTCAAATGGACAATGATCCTTATACTTTGGTGGTAGCTTTGAACAGTTCTCAAGAAGCA GAAGGTGAACTCTACATCGATGACGGCAAAAGCTTTGAATTCAGACGAGGCTCTTACATCCATCGTCGCTTCGTCTTCTC AAAGGGTGTTCTTACATCAACGAACTTAGCTCCTCCAGAAGCTCGTCTCTCTTCCCAATGCTTGATCGACAGAATTATCC TCTTGGGACACAGCTCAGGTCCAAAATCTGCGTTGGTGGAACCGTTGAATCAAAAGGCAGAGATTGAGATGGGACCTCTG CGAATGGGTGGGCTTGTAGCTTCCTCGGGTACAAAGGTGTTGACTATCCGCAAACCGGGTGTTCGAGTGGACCAAGACTG GACCGTAAAGATTCTGTGATTGAACGGTTTGAACCAGTTTCACTCATGGCCGTTAGAGTGGCCGAAATCTGCTTTTCCGG CGACGGAATATCACACTTTTTAATATATGTTTGGAGAT ................................................................................ ................................................................N............... ...N................................................N........................... ....................................N................N.......................... ................................N............................................... ...............................................N..............N................. ..............................N...............................................N. ......................N...............................................N......... ................................................................................ ......N........N.........................................N...................... ............................N................................................... ..............................................................N...........N..... ............N................................................................... .........................N.................N.................................... ...N........N.......................................................i........... ...........................................N..............N..................... ....i...............................N...................N........N.............. ............N.................N..N.............................N................ ..i.................................i................................i.......... .......................N..................................N.........i........... ..................N.....................................i......................i ...............................N................................N....i...N...... ...........N................N.........N........N................................ ......N................i............N..........i................................ ....................................................................N........... ................i............................................................... .....................................................................N.......... ...................................................N......N..................... .....................................................................N...i...... .........N....................N..........i...................................... ............................................N....i.............................. ................i...N........................................................... ..........................i......i.............................................. ...................N............................................................ .........................................................i...................... ....................................................................i........... ...i............................................................................ .............................................N.................................. ..........................N........... Pos Score Pred ------------------------ 145 0.238 - 164 0.364 - 213 0.153 - 277 0.389 - 294 0.476 - 353 0.085 - 448 0.141 - 463 0.140 - 511 0.198 - 559 0.345 - 583 0.145 - 631 0.122 - 727 0.163 - 736 0.192 - 778 0.286 - 829 0.077 - 943 0.271 - 955 0.146 - 973 0.248 - 1066 0.183 - 1084 0.104 - 1122 0.797 Yes 1132 0.178 - 1171 0.215 - 1182 0.410 - 1192 0.362 - 1204 0.329 - 1213 0.246 - 1269 0.746 Yes 1324 0.443 - 1339 0.256 - 1365 0.642 Yes 1397 0.281 - 1417 0.445 - 1426 0.323 - 1453 0.211 - 1471 0.194 - 1474 0.185 - 1504 0.169 - 1523 0.732 Yes 1557 0.582 Yes 1590 0.628 Yes 1624 0.392 - 1659 0.369 - 1669 0.548 Yes 1699 0.459 - 1737 0.653 Yes 1760 0.614 Yes 1792 0.345 - 1825 0.405 - 1830 0.776 Yes 1834 0.376 - 1852 0.120 - 1869 0.429 - 1879 0.398 - 1888 0.410 - 1927 0.255 - 1944 0.810 Yes 1957 0.305 - 1968 0.668 Yes 2069 0.429 - 2097 0.505 Yes 2230 0.252 - 2292 0.453 - 2299 0.425 - 2390 0.399 - 2394 0.765 Yes 2410 0.216 - 2431 0.335 - 2442 0.556 Yes 2525 0.488 - 2530 0.582 Yes 2577 0.700 Yes 2581 0.320 - 2667 0.780 Yes 2674 0.607 Yes 2740 0.359 - 2858 0.697 Yes 2949 0.601 Yes 2964 0.592 Yes 3086 0.079 - 3147 0.053 -

Explain the output -- Processing locus 38: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATCAATTTGATTAAATCAAAGGACTTGATTGATTTTGTTTTTGAGGTTCACACGGTTCAATCAAGATCATCGAAATCCG AAATTCCAAAGTGCGATTTAACCGATTAAACCGTCCGGTCCATTGGTTCAGGTCCTTACCTCATTTATAAGAAGAAGGAA GGGTATTCCCGTAAATAATATGGAATTTGATGTCCAGTTAAAAAAACTCCAAATAGGCCTCTCAGCTAAAAACACCGTTT CCATCTCTCCGGCTCTCTCGCCATCGGATCCCTTTCTCCGCCATTTCACACTGCTCAACGCCGCCACTTTCCTTCTTCGA TCGACTCTTCATCTCCTTCTCCTTCTTCTTCTTTACACTTTCCAATCAACTCGATCAGATTTTTTCTACTTGGAAAGCGA CGAGACTGTTGATTTTTCCGGCTGCCGGAATCAGCGCTTCGAGGAGAAGATGTTAGGGTTTTCAAATGGAAGCGGTGGCG GTGGAGTTGGAGCTGAGGATTTAGCTCAACTCCAATCTACCATGCGCGCGATTGAACTTGCTTGCTCTTACATTCAGATT AATAGTAATCCAGTTGCTGCTGAGGCAACCATTTTGTCGCTTCACCAATCCCCACAGCCGTATAAGGCGTGTAGATATAT TCTTGAAAATTCTCAGGTGGCAAATGCTAGGTTTCAAGCTGCTGCAGCTATTCGAGAATCAGCTATCAGGGAATGGAGCT TTCTTGCCACCGATGATAAAGGGGGCTTGATTAGCTTCTGCCTTGGCTATGTCATGCAGCATGCTAATTCATCTGAGGGA TATGTGCTTTCAAAAGTATCTTCTGTGGCTGCACAGCTGATGAAAAGAGGCTGGCTTGAGTTTACTCCAGCCCAGAAGGA AGTCTTTTTCTATCAGGTATCTGAATTCTCACCCTCTACTTCAAGTGCCATGGGTCTCCCTAGGGAATTTCATGAAAATT GCCGCAAGTCACTAGAGCAAAACTTTTTGAAGAGTTTCTATCAGTGGGCACAAGATGCTGCTTTGAGTGTTACAAGCAAG ATTATTGAATCTCATTCTAGTGTCCCTGAGGTTAAGCCTGGAGCTTCATGGTGTGATGTTTTGCTTTCAAGCTCACATGT TGGTTGGCTGATAAATTTCTATTCTTCGGTGAGGCAGAAGTTTGATCTTGAAGGTTATTGGCTAGATTGTCCTGTTGCAG TCTCTGCTCGGAAACTTATCGTGCAATTGTGCTCCTTGGCAGGGGAAATATTTCCATCGAACAATGTGCAGATGCGTGAT CAACATCTGCTCCTCCTGCTGACGGGTGTGTTGCCCTGGATTGATCCTCCTGATGTCATTTCAAAGGAAATTGAAGAAGG AAGGAGTGGAAGTGAGATGATTGATGGTTGCCGCGCATTGTTATCTATTGGGACTGTTACTACTCCTGTTGTCTTTGACC AACTCTTGCGATCATTAAGGCCCTTTGGCACCCTTACACTCTTATCTATGTTAATGGGTGAAGTTGTCAAAGTACTAATG GCGAATAGTACTGATGAAGAGACTTGGAGCTATGAGGCACGCGATATCTTGCTAGATACCTGGACCACACTCCTTACTTC AATGGATGGTTCTGGGGGTAATGCGTGGCTACCACCTGAGGGGATACATGCTGCAGCTAGTCTCTTTTCATTGATTGTGG AATCTGAACTAAAAGTGGCATCTGCGTCAGCTACTACTGAAGATGATGCTGACTGCCTGGCTTCTGTATCCGCCATGGAC GAAAGACTAGGCTCTTATGCTCTCATTGCAAGGGCAGCAGTCGATGCTACAATTCCATTTTTAGCAAAACTTTTCTCTGA TCATGTTGCACGCCTCCATCAGGGCAGGGGCACTGTTGATCCGACTGAAACTTTGGAAGAGGTCTACTCTTTGCTGCTAA TAATCGGCCATGTACTTGCAGATGAAGGGGAAGGGGAGACTGCCTTGGTTCCTGATGCTTTGCAATCTCATTTTGTGGAT GTTGTAGAGGCAAACAACCACCCTGTCGTGGTACTCTCAAGCTCAATAATAAAATTTGCCGAGCAATGTTTGGATGCAGA AATGAGATCATCGATCTTTAGTCCTCGGCTAATGGAGGCTGTCATATGGTTCCTTGCAAGATGGTCTTTCACATATCTAT TGCTCGTTGAAGAGTGCAATTTGGGTAGCAACAAGCTTCAGTCTCTACCTTCAAGAGCATGTTTATTTACGTATTTCAAC GAACACAATCAGGGGAAGTTTGTCCTTGATATAATTGTCCGGATCTCCTTGACATCGCTCACATCTTACCCTGGTGAAAA GGACTTGCAGCGTTCATTGGCTCAAACCCTTGTTCTTTCTGCTTATGGAATGCGAAGTTCAGATGCATCAAATCAATATG TGAAGGACCTCATGGCTCATATGACATCATCTCTAGTGGACTTGTCCAATAGTAGTGACCTTAAAAATTTGGCTCAACAA CCTGATATTATCATGCTGGTGAGTTGTGTGTTAGAGCGGCTTCGAGGAGCGGCAAGTGCCACTGAACCCCGAACACAGAG AGCAATATATGAGATGGGATTGTCTGTCATGAATCCTGTACTCCGTCTTCTCGAGGTTTACAAACATGAGGTTGATTTTT CATCTGATAGTATTGAGACACAAAGTACAAATATTTCCCAGGTGGTGTACTTTGGTCTCCATATAATCACTCCACTTATA ACTTTGGAACTCCTCAAATACCCCAAGCTTTGCTTCGACGATGTAGATATAGTCACAATGTGCCTGAGAGCTCTAAAGGC TCTTGCTTCATATCACTACAAAGAGAAAAATGCTGGCAACTCGGGTTTGGGTTCACACGCTGCTGGACACACAGATCCAA ATGGAGTATTCCATGAAGGAATATTGAGTCGATTCCTTCGTACATTGCTTCACTTCCTTCTTTTTGAGGATTACAGTACC GACCTAGTCAGTACAGCAGCAGATGCCTTGTTCCCTCTTATTCTCTGTGAACCAAATCTTTACCAGGGATTGGGCAACGA ACTGATTGAGAAGCAGGCGAATCCGAACTTTAAAACTAGGCTAGCAAACGCGCTTCAGGTGCTCACAACGTCAAACCAGC TATCCTCATCGTTGGACCGTCTCAACTACCAAAGATTCAGAAAGAACCTCAACAACTTCCTTGTCGAAGTTCGTGGGTTT CTGAAAACTAGGTGACCGAATTTTAGAACTTATTTGCTTTTTTTTTGGGTTGTAAAAAGAATTTACTTGGTAGAAGTGGT TTTTTCCGTTTGTAACAATTAAATTTTACTTTTA ................................................................................ ................................................................................ ...................i.........N.................................................. ................................................................................ ................................................................................ .................................................i...............N.............. .........................................i...................................... ................................................................................ .......................N................................................N....... ............N...................................N....N......N................... .................................................N.....................N........ ......................................................N......................... ...............................................N.......N....................N... ................................................................................ ...............................................................i.......i........ ....................................................N........................... ................i......N........................................................ ...............................................N.....i.......................i.. .............N.................N................................................ ..........................................N..N............................i..... ................N..........................N.................................... ..N............................................................................. .........N...........N................................N.......................N. .................................................................i.......N...... ..........................................................N..................... ................................................................................ ............................................N....N............N..............N.. ...........N........N........................................................... ............N................................................................... ........N....i..............i....................................N.............. ................................................................................ ........................................N................i...................... .............................i.................................................. i...........N................................................................... ......................N......................................................... ................................................................................ ................................................................................ ................................................................................ .................................. Pos Score Pred ------------------------ 180 0.501 Yes 190 0.313 - 450 0.582 Yes 466 0.359 - 522 0.768 Yes 664 0.467 - 713 0.304 - 733 0.210 - 769 0.316 - 774 0.418 - 781 0.223 - 802 0.174 - 840 0.462 - 930 0.491 - 952 0.154 - 1015 0.268 - 1088 0.202 - 1096 0.107 - 1117 0.206 - 1264 0.525 Yes 1272 0.673 Yes 1333 0.135 - 1377 0.587 Yes 1384 0.143 - 1488 0.440 - 1494 0.550 Yes 1518 0.754 Yes 1534 0.403 - 1552 0.397 - 1602 0.618 Yes 1606 0.084 - 1621 0.170 - 1648 0.246 - 1723 0.109 - 1726 0.276 - 1755 0.689 Yes 1777 0.111 - 1804 0.130 - 1843 0.189 - 1930 0.190 - 1942 0.485 - 1975 0.118 - 1999 0.093 - 2066 0.563 Yes 2074 0.156 - 2082 0.224 - 2112 0.393 - 2126 0.363 - 2141 0.310 - 2219 0.438 - 2365 0.255 - 2370 0.359 - 2383 0.380 - 2398 0.341 - 2412 0.394 - 2421 0.479 - 2493 0.424 - 2569 0.197 - 2574 0.571 Yes 2589 0.594 Yes 2626 0.163 - 2761 0.292 - 2778 0.728 Yes 2830 0.619 Yes 2881 0.514 Yes 2893 0.158 - 2983 0.391 -

Explain the output -- Processing locus 39: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCCCCCTACCTTGATCTCTGAATGCCAGTTTGATGCGTCATGGCATGGAAATGATCTACTTAGCAGTCATGGATCTCGTA ATAGCCTTTTCTCGCATGTAAACTTATTGGTTCCTGAATGGCTATGAAATAATCTTTGGCATGTGTTCGAGAGCCCATAG AGTCATGTTCAGATCGAACGCAAGAGCGTTGAATAGAGCATTGGAGAGACTAAAGAATGTGCAGACCAAGGTGAATGAAG CTCTTAAGAGATCAGGGATCCAAGAGAAATCTTTGGAGCGAAAGCTGAGGATATGGCTAAGAAAGGTCGAAGAGAATGTG CCTCTTGGTGAGCTGATTTTGGAGAAACGCTCTTCATGTGCTATATGGCTGAGCGACAAAGATGTGGAAATTCTTGAGAA AGTCAAAAGGCTGGAGGAGCAAGGCCAAGATCTCATAAAAAAGATTTCAGTCAACAAGTCATCTAGAGAAATAGTTGAAC GGGTCTTGGGGCCTTCATTTCATCCTCAAAAAACAGCACTGGAGATGTTGGATAAGCTAAAGGATTGTTTGAAGAAGAAG AATGTTCAAAAGATTGGTGTTTGGGGTATGGGCGGTGTTGGGAAGACAACACTTGTCAGGACGCTTAACAATGATCTCTT GAAATATGCTGCTACTCAACAGTTTGCTTTGGTAATTTGGGTGACAGTGTCGAAAGATTTTGACTTAAAAAGGGTCCAGA TGGACATAGCCAAGAGACTGGGCAAGCGATTTACAAGGGAGCAAATGAATCAGCTAGGCTTAACTATTTGTGAGAGGCTA ATAGATTTGAAAAATTTCCTCCTTATTCTTGACGATGTTTGGCACCCCATTGATTTGGACCAATTGGGTATTCCATTAGC ATTGGAACGATCGAAGGATTCAAAGGTTGTTCTAACTTCAAGAAGACTAGAAGTGTGCCAACAGATGATGACTAATGAAA ACATCAAAGTTGCATGCTTACAAGAGAAAGAAGCTTGGGAATTGTTTTGCCACAACGTTGGAGAAGTAGCAAACTCTGAT AATGTTAAGCCCATTGCAAAGGATGTATCCCATGAATGTTGTGGATTGCCTTTGGCTATCATCACAATCGGGCGGACCCT GCGCGGAAAACCTCAAGTCGAGGTATGGAAACACACCTTGAATTTGCTAAAGAGATCAGCACCTTCTATCGATACTGAAG AAAAGATCTTTGGTACTTTAAAACTCAGCTACGACTTTCTTCAAGACAATATGAAAAGTTGCTTCCTTTTCTGTGCTTTA TTTCCGGAGGACTACTCTATCAAAGTAAGTGAACTAATAATGTACTGGGTCGCGGAAGGATTGTTGGATGGACAACATCA TTACGAGGACATGATGAATGAGGGAGTTACTTTGGTTGAGAGATTGAAGGACTCTTGCCTGTTAGAAGATGGTGATAGTT GTGATACAGTTAAAATGCACGATGTTGTGCGTGACTTTGCTATATGGTTCATGTCTTCTCAGGGTGAAGGATTTCATTCT CTTGTCATGGCCGGGAGAGGTCTAATAGAGTTTCCACAAGATAAGTTTGTTTCTTCAGTCCAGAGAGTTTCTTTGATGGC TAATAAGCTAGAAAGACTACCTAATAATGTGATAGAGGGTGTGGAAACATTAGTTTTACTACTACAAGGGAATTCCCATG TTAAGGAAGTTCCTAATGGGTTCTTACAAGCATTCCCAAACCTTAGAATCTTGGATCTCAGTGGTGTTCGTATAAGAACA TTGCCAGACTCCTTTTCAAATCTTCATAGTTTGCGGTCTCTTGTGTTAAGAAACTGTAAAAAATTGAGGAACCTGCCTTC GCTAGAATCCCTTGTTAAACTCCAATTTCTTGATCTACATGAATCTGCCATTAGGGAACTTCCGAGAGGTCTTGAAGCGT TGAGCAGCTTAAGATACATTTGCGTTTCCAACACATATCAACTCCAGAGCATTCCGGCTGGAACTATTCTCCAGTTATCA AGTTTGGAGGTTCTAGACATGGCGGGGAGTGCTTATAGTTGGGGCATCAAGGGAGAAGAAAGAGAAGGACAAGCAACACT TGACGAGGTTACATGCCTCCCTCATTTGCAGTTTCTTGCCATTAAGCTCCTCGATGTCTTGTCCTTTTCCTATGAATTTG ATTCTTTGACCAAAAGGCTAACAAAGTTCCAGTTTCTATTTTCACCTATTCGATCGGTTTCACCTCCTGGCACCGGTGAA GGTTGCCTAGCAATCAGTGATGTCAATGTCTCAAATGCATCTATTGGGTGGCTACTGCAACATGTGACATCACTAGACTT GAACTATTGTGAGGGTCTTAATGGTATGTTCGAGAACTTGGTAACGAAGAGCAAAAGTAGCTTTGTTGCCATGAAAGCAC TGTCCATTCACTATTTTCCCAGCTTAAGCCTTGCGAGTGGTTGTGAGTCCCAACTGGATCTCTTTCCAAATCTCGAGGAA CTTTCTTTGGACAATGTCAATCTGGAAAGCATCGGAGAACTTAATGGTTTCCTCGGGATGAGACTTCAGAAACTAAAACT ACTTCAAGTCTCTGGCTGCCGACAGCTTAAACGTCTTTTCTCAGACCAAATCTTAGCAGGTACTCTGCCAAACCTACAAG AGATAAAGGTTGTCTCATGTTTGAGGTTGGAGGAGCTCTTCAATTTTTCTTCAGTGCCAGTTGATTTTTGTGCTGAGTCT TTGCTACCTAAGCTAACTGTCATAAAACTCAAATATCTTCCACAATTAAGGAGCTTATGCAACGACAGAGTGGTGCTCGA ATCCCTGGAACACTTAGAAGTCGAATCTTGTGAAAGTTTAAAGAACTTACCCTTTGTCCCCGGCAATACTGGTATGATCA ACGAACAAATGGCGTGGGAATACATGTCTAGGACTCTAGGGTAAAAGATGATCCAGAATGGGACCTCCAAATTTTCTTCC AAAAAAAAAAAGAATGGGACCTCCAACCTTGCGACATTAAAAGTGAGAATTAGTTACATTTTT .....................N..........N......i....N.....N.................N........... ...............N.....................i.....N................i................... ....i...................................................i.................N..... ....................................................i......................N.... ...................................N........i................N.................. ................................................................................ ............................................i................................... .....N.........................................................................N ............................................N................................... ..................................N............................................. ................................................................i..i......N..... .............N.................................................................. ........................N....................................................... ..................................................i............................. .......................................i...........................N............ ..........i..i...N..................................................N........... ..............i......N.....................N......i............................. ......i....................................................................i.... ..........................N..................................................N.. ...............N................................................................ ................................................................................ ......................................N......................................... ................................................................................ ..................i............................................................. ............N........................................i.................N........ ................................................................................ ...................N.....N........N..........................N.................. ....................N....N............................................N......... ................................................................................ .............N.............................i.............N...................... ................................................................................ ................N............................................................... ........................................................N....................... .........................................................................N...... ........i..............i.......................N.........N...................... .............N................................................. Pos Score Pred ------------------------ 22 0.381 - 33 0.339 - 40 0.522 Yes 45 0.367 - 51 0.305 - 69 0.230 - 96 0.448 - 118 0.699 Yes 124 0.136 - 141 0.679 Yes 165 0.586 Yes 217 0.568 Yes 235 0.146 - 293 0.641 Yes 316 0.315 - 356 0.494 - 365 0.527 Yes 382 0.248 - 525 0.562 Yes 562 0.223 - 588 0.548 Yes 631 0.216 - 646 0.310 - 720 0.354 - 765 0.292 - 835 0.343 - 945 0.610 Yes 948 0.832 Yes 955 0.240 - 974 0.204 - 1042 0.349 - 1063 0.365 - 1072 0.221 - 1076 0.574 Yes 1145 0.243 - 1251 0.589 Yes 1320 0.527 Yes 1348 0.466 - 1371 0.640 Yes 1374 0.782 Yes 1378 0.285 - 1429 0.153 - 1455 0.719 Yes 1462 0.262 - 1484 0.234 - 1491 0.532 Yes 1527 0.517 Yes 1596 0.531 Yes 1627 0.381 - 1678 0.361 - 1696 0.357 - 1879 0.304 - 2019 0.754 Yes 2093 0.297 - 2134 0.593 Yes 2152 0.138 - 2260 0.221 - 2266 0.360 - 2275 0.279 - 2302 0.143 - 2341 0.169 - 2346 0.217 - 2391 0.292 - 2494 0.275 - 2524 0.556 Yes 2538 0.259 - 2657 0.352 - 2777 0.110 - 2874 0.157 - 2889 0.774 Yes 2904 0.546 Yes 2928 0.173 - 2938 0.121 - 2974 0.135 -

Explain the output -- Processing locus 40: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGCCAGGAAAGTAGGACTGATTTGCCTTTGCTGTTTGATTTATTACATTCTGAGTTTACCACATTTTTGGGTTCTTGAA AGTTTACATTTTCAGGTTTGAGATCCAATGATGTTGAAAATGCAGTTCTTTTTTGCTTGTTCAGGTTTCACTACGTTGAG GATGGTTCTTCTGATGGATGGGGAAAATAATCTTATGGAAGAAACAACAGCTGTTATCGAAGAAGACTCCTATGATGACG AGTTTCCCTGCGGAGATCCACAAGTTGAGCCTCGTGTTGGAGATGAATTTCAGGTCGACATTCCTCTTATGATGTCTGCA TCCAAGCGCGCTGTGTTTCTTTCCAATCCTGTTGCTTTGGATGATTCTACATGTTCTTTTCTCGTCGGTCTACCTGTCCA AGTAATGTGGATAGACAAAGTTGGAATAGGACAAGGAAATGGAGATGGCAATGTTGACATGAACCAATCTTTGAAATCTT TAAGAGCTAAAAAAGGCCGTTGCTCTGCCAAGATCAGAGGAAAGAGTGACAAGAATTCGGAAACCAAGAAGCAAAGACTG AATCTTGAGGCTGTTCCTGCGATACCTTCCAGTTCTTGGGACGATTTGGAGGTTGCTAGCTTTGTTCTTGGTCTGTACAC ATTTGGGAAGAACTTTACTCAGATGAATAATTTCATGGAGAACAAAGGAATAGGAGAGATCATGTTGTTTTACTATGGGA AGTTCTACAATTCTGCAAAGTACCACACTTGGTCTGAATCCCGAAAGAAGCGCAACCGCAAATGTGTATATGGAAGAAAG CTCTATTCAGGTTGGAGGCAACAACAGTTGTTAACCCGTTTGATGCCTTCTATTCCTGATGAACCTCAGAAACAGATGCT TGTGGATGTCTCAAAGTCATTTGCTGAAGGTACTATCACTCTAGAGAAATACGTAAGCGCAGTGAAGAATCTTGTGGGTC TCAGGCTTCTGGTAGACGCTGTGGCGATTGGTAAAGAAAAAGAAGATCTCACGGTTCCTACATCAACCCCAATGAAGACC AAACCATGGTTCACTGTTTCTTCAAAATCTTCTTTGGTCCCTGGAGAAGGGGATTACAATTCCCTCACATCGGCTGGCAT AATAAATCAGTTAACTGGCTGTTCTCGTCTGAGCAAAGCCCGTTGCAATGATATATTCTGGGGCGCTGTATGGCCGCGTT TGCTAGCCAGAGGATGGCGTTCACAGCAGCCCGAGGACCGAGGCTATTTCAAATCTAAGGACTACATTGTTTTCATTGTC CCTGGCGTGAAAAAGTTTTCAAGACAAGAGCTTGTCAAAGGAGATCATTACTTTGATTCCGTCAGTGATATTCTAACAAA GGTTGTGTCAGAGCCTGAACTTCTTGAGAATGAAACAGGAGGAGTGGCTGCAGAGAATCCTTCTGACCAGTCAGATGAAG AGTCTTCCCCGTCTGATAGTCTAAGACACCGTTACCTCAGGTCTCCATGTTCTAACCGTGGAACTCTGGGAATGAAATTC ACTGTTGTGGACACTAGTTTGGCTACTGGAGGAAAGTTGTGTGATTTACGGAATCTGAATGCAGAGTGTTTAGTAGTTTC TGAGCCAAAGGCTCGTCTAGAAGCTAAAGATTCTTCTGTGCTTAAAAATTCTCTGGACAGTCAGAATGTGGAAAAGTCTC AAGTGAGGCCTCTGGATGCCAAGAACCATGTGGATGACCCAATGCGGTTCACGATTGTTGATACGAGTGTGGACCACTGC GAAAAATTATCTGGGTTTAGGAGATGGAGATGTTTACCAAGTGATGACACAAGAAGGGGTCATGTAGGGGCTGATTCTGG TATAAAGGAAGAGAAGACTTTAGAGAAGGCTAAGGATCCATCAAAGAGGGTTATTAAGCCTCGGTCTACCCCTCGAGCAG AAACTAATTATTATGCTGTAGATTCTGCACCATATTTGAAACGCAGACGGCTCAGTGCTTGCATTTCCAGAGAGAGTCCA GTGTCCAAACATTTACCAGGTGATAATGACACAAAAATGACTATTTGTCTTGAATCAGAGCAGCAAAGTATATGTGTGGT CCAACAACAAACTAGCACTTGTGAAGAGATGAATCAGGACAAAGAGATTGTGCCGTTGGTTGAGCATATGAACTTAAAGT CTGATCAATCAAAAAAGACTGGAACTGGGCTATCTTCTTCGCTTGTTGAGATTCAAGAAACGACAGCGATTGAACCAAGC GGATTAAATTCCAACACTGGTGTCGACAAGAACTGTTCACCAGAGAAAATAAGAACTGCCCATGAACTCATTTCGGCAGA ACCAAAAACAAATGGGATCTGTTCAGTGTCAGAGTTGGACAAAAAACGAGCTTCCAGTGATCTGGAACAAAAACAAGTAT TTGAGCTTCCTTCCATTTCAGGCTCAAACAATAGATCTCCTTCCAATGATCTGGGAACTTCTCAAGAAATGGGTTCATCT GAACAACAACACAACCAACAAATTAAGACAGATGGTCCTAGAAGACAGAGCACAAGAAAGCGACCATTGACCACTCGGGC TCTGGAAGCTCTGGAATCCGACTTTCTTATAACCAAGAGAATGAAAAGCACAACTAAACCAGAACCGAGAAAACATGAAA GTTCAACGAAGAAAAAGCGCTCAGCTAAAGCATGTAACAGAAATGGGAGTGCAGACTTGGAGCACAGAGGAGAAGATAGA AGCAGTTTTACAAAGAAATCACCAACAAGTAAGCCCTTGGATCAAATAGAGGATTCAAAGCCTAGCTATCTTCTCAATGA AGCAAGAACTGAGAGTAAGGCTCTGGATCAAGTACAGGAGTCAAGGCCGGTTCTAACTGAATACCGTAAACTTCCACCGA TTGTATTGAAGCTTCCATTCAGGCGTGGCTAAGGAGCTCAACGGACTTAAGTCTGAAAGTTTTGATCGCGGGATGTGAGA AGATTGGCCTGGCTGTATGCTGAAGAATAAGTGTGTACAGAAGAGCGATAT ................................................................................ ...........................i..i........i........................................ ..........................................N.........................N..N........ ........................................N.........N............................. ....N.................................i.....i.....N.......N..................... ................................................................................ ................................................................................ ......................N...........N..........................i............i..... .............................................................N.......N.......... ..........................................i...............N................i.... .....N.......................................................................... .......................................................................N........ .....N.......................................................................... ...............................................i.....................N.......... .............N.................................................................. ................................................................................ .............................N............................................N..... ..............................................N........................i........ ..........................................................N..................... .................................................................N.............. ...............N...........i.....N.......N...................................... .......................N.....N.............N.................N.................. ................................................................................ ............N................................................................... .........................N..........i..................................i........ ............................i......................................N............ ................................................................................ .............................................................N.................. ...........N.................................................................... .............................................i......................i........... ...............................N................................................ ........................................N.................................i..... ...............................N..........i..................................... ............................................................................N... ................................................................................ ........................................................................N....... ................N.................................. Pos Score Pred ------------------------ 108 0.569 Yes 111 0.794 Yes 120 0.662 Yes 162 0.793 Yes 174 0.768 Yes 178 0.215 - 195 0.593 Yes 232 0.137 - 235 0.242 - 283 0.056 - 309 0.390 - 312 0.490 - 361 0.201 - 371 0.134 - 405 0.481 - 439 0.825 Yes 445 0.630 Yes 451 0.316 - 459 0.208 - 663 0.306 - 675 0.338 - 702 0.625 Yes 715 0.562 Yes 782 0.457 - 790 0.289 - 843 0.585 Yes 859 0.099 - 876 0.643 Yes 886 0.161 - 1032 0.277 - 1046 0.307 - 1168 0.620 Yes 1190 0.312 - 1214 0.350 - 1390 0.259 - 1435 0.302 - 1487 0.308 - 1512 0.727 Yes 1579 0.314 - 1666 0.155 - 1696 0.402 - 1708 0.596 Yes 1714 0.270 - 1722 0.450 - 1784 0.208 - 1790 0.271 - 1804 0.371 - 1822 0.198 - 1933 0.302 - 2026 0.438 - 2037 0.607 Yes 2072 0.680 Yes 2109 0.697 Yes 2148 0.326 - 2302 0.160 - 2332 0.328 - 2446 0.554 Yes 2469 0.787 Yes 2512 0.328 - 2601 0.476 - 2635 0.656 Yes 2672 0.145 - 2683 0.561 Yes 2797 0.182 - 2953 0.046 - 2977 0.052 -

Explain the output -- Processing locus 41: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGCATGTGTTAGTTAGATCCTCTAGTTGTCTATCTTTGCTAGGTAATGTCTAGTGTTCTTATTTAGTCTCAGATGAATCT TTCTCTTGTATGGATGCATGATCTTTGTCTCTCTTAGCCTTTTGTTCTCTGCTCAGTTACTTTAGTATGTTCTTGAATCA TATGGTTTTATTTGTGCAGACAGTTCTTATGATGGAAGAAGAAAACAGTTCTATGGAAGAGTCTTGTGATGAAGAATTCG TTTGTGGAGATCCAAAAGTTGATATTCGTGTTGGAGATGAGTATCAGGTTGAGATACCTCCTATGATGTCTGAATCCCAA CGTGCAGAGCTTCTTTTGAATCCTCTGGAATTTGATTCCTCTTGTTCTTTTGCTGTGGGACTACCTGTTGAAGTAATGTG GATCGAGACAAAATGTAGAGATGGAGATGGACTTGGAAGTGATAACATTGACATGAACGAATCTCTCAAATCTCTTAAAA GAAAAAGAAGTCGCAGAGGTGGGAGTGACGGGAACTCGGGTTCTAAGCGACGTATGAATCTTGAAGCTGTGCCTGAGAAA TCGTCCAGTTCTTGGGAAGATTTGGAAGTAGATGGGTTTGTTCTTGGACTGTATACATTTGGGAAGAACTTTGCTCAGGT GCAGAAGTTGTTAGAGAGCAAAGCAACAGGAGAGATACTTTTGTTTTACTATGGAAAGTTTTATGGTTCTGCTAAATACA AAACTTGGTCTAATTATCTTAAGAAGCGGAGTACGAGATGTATACAAGGGAAGAAGCTCTATTCAGATTGGAGGCTACAG CTGTTGTTGTCCCGTTTAATTCGCAGCATTACCGATGAATCGAAAGAACAGAAGCTAGTGGATGTCTCAAAGTCATTTGC TGAAGGGAAGAAATCTCTGGAGGAATACATCAACGCGGTTAAGAAACTAGTGGGTCTTCGGTGTCTTGTAGAGGCTGTAG CAATCGGTAAAGATAAGGAAGATCTGACAGTCCTTACAACAAAACCCGTAGATGTAGAACAATGGTTTAGAGTTTCTTCT GCGGTACCTGCTGGTTTAGGGGAATACAATTCACTCACAGTTGAAGGTATAATCGAAAAATTGTCTGGTGGTTCCCGTGT GAGCAAAGCTCGTTGCAATGATATCTTCTGGGATGCTGTATGGCCGCGTTTGCTACATAGGGGTTGGCGTTCTGAGCTGC CAAAGGACCAAGGTTACATCAAGTCTAAAGAACACATTGTCTTCCTCGTCCCTGGGGTAAAGAAGTTTTCAAGAAAGAAA CTTGTGAAACGTGATCATTACTTTGATTCTATCAGTGACATTCTCAAAAAGGTTGTTTCAGAGCCTGAGCTTCTTGAGGA GACAGCAGAAGAAGAAAGAGAAGAGAACACTTACAACCAATCAAAACAGGAGAAACACTGTTACCTCAGGTCTCCAAGCT CTTCTTCTACTCATATGAAGTTCACTGTTGTGGACACTAGTCGTTTTGCTTCAAGAGGAAAGTTGTACGAATTTCGGGAG TTGAGGATTCCAAGTTTAGCTTCTCAATCAAAGGCTTGTCGAGGGGATAACAACTCTTCTGTGGAAAGATTCAAGTTTGC TGATGAACGTAAGTGTAAAAGGAAGCAAAAGATGGAAGTGGTGGATGAACCGATGACATTCTTGATTCTGGATACAAGTG TGGACAAAGGTGGGCATACATCTGGGATAAGAAGACGGAGACATTTGCCTAAAGAGGCATTTGGTGAATCATCACAGAAC CAATCTGGCACTAGTAAAGATGTGAACTGCGAGTATCTTAAGGGGACTGATCCTGGTGTAGAGGAAGAGACTTTGGAGAA TGTCCAACAAGGTCGGTCAAAGAAGATTAAGCAGAAGTTTGCCCTATTATCAGAATCTAATAAACGTCATTTGGTTGGTT CTCTTCCCTTGCGAAAACGTAGGCGGCTCAGTACTTGTGTAAGGAAAGATAGAAAACGTTCTGGTGAAAGTTCAGTGTTG AAGCCACCTCCATTAGACCAGATAACTAACAGCCACCCCAAGTTACATGTTGATTCTATGAACCTCAACACTAACCAGTC AGAAGAAAATGAAAATATTGAGATTCAAGAAAGGCCAGAAACTGAACCAAATGGCTTTTGTTCCATCTCGGAGACAGTTC ATGAACCAAGTTCATCAGCACAACAACAAGAACCAAATGGGTTACGTTCATCAAAGGAACAAGGAGCTCTTCATGATGAA CCAATTTCACTAGCACAACAACAAGAACCAAATGGGTTATATTCATCAAAAGAACAAGGAGCTTTTCATGAACACAGTTC AACAGAACAACAACAAGACGAATCAAATCGGTTGTGTTTGGACAAAATATGCTCTTCCAAGGATCTGGGAACTGCTCAAA AAGAAGAGCAACCGATTCAGTTGCCTCCTAAGTCAGCCTCAGACAAGAATTCTCCGTCCAGAGATCATGGAACTACTGAA GAACGTGCATCATTAGAACAACAAGAAGAAGAGCATAAACAACAGGCCAACACGGACATTCCAAGAAGACAAAGCACAAG AAAGCGGCCGTTGACTACTCGTGCTCTGGAAGCTCTAGAATCGGACTTTTTTACACCAAAAAGCAAGAAAACTACTACAT CTAAACCGAGAAACCGCGAACGTTCTGCAAGGATCAAGCACTCAGCCAATGCGAATAACAAAACTCCAGGCGAGTCAGAC AATAGTTTTCATGTAAAGGAGGCGATAACAAGTAAACCGTCAGACCAAATAAAGGATTCCGAACCGAGCTTTCTTGTGGA TAAAGTAACGGTGACAAGTAGTAAGCATGTGGAACAAATAAAGGATTCGGAGAAGGTGACTACCGAGTTCCCTAAACTTC CTCCAATTGTTTTGAAACTTCCCTTTAGGCGTGGCTCAAGAAGCAATGAAGACTGAAGTCTGAATCATTTTTTGCGCGTA GTTGACTCAATGAAGAAGGGTGTACAGAAGAAGAGAGCTCTTTAGGAAGAAATTTTTTTTTGTTTGTTTCTCTGG ...N.........................................N..........................N....... .........N...N...N................................................i............. ....................................N.........................N..i.............. ...........................................................................i.... ............N.......i.....i.........................i........................... .....................................................N.......................... ...............................N................................................ ..................................................N...........N................. .....................................i.......................................... ..................................N..........................N.................. ................................................................................ ...................................................N.........N.................. ................................................................................ .................N..............N......N........................................ ................................................................................ ................................................................................ ................................................................................ ..............N................................................................. ................................................................................ ..N............................i............N.......N........................... ................................................................................ ...................N...........................................................N ................................................................................ ................................................................................ ..............................................N..........N...................... ........N.........................................i............................. N...................................i...................................N..i.... ...............................i...................................N............ ................................................N............................... ..................................................................i............. ................................................................................ ................................................................................ ................................................N............................... ..........N..................................................................... ..........................N..................................................... .............................................N.................................. .........N................................................................. Pos Score Pred ------------------------ 4 0.434 - 46 0.277 - 73 0.084 - 90 0.203 - 94 0.155 - 98 0.426 - 147 0.556 Yes 162 0.562 Yes 189 0.531 Yes 192 0.727 Yes 213 0.588 Yes 229 0.107 - 277 0.080 - 303 0.392 - 306 0.702 Yes 396 0.758 Yes 413 0.444 - 421 0.623 Yes 427 0.628 Yes 453 0.530 Yes 534 0.324 - 592 0.126 - 691 0.231 - 703 0.331 - 758 0.601 Yes 835 0.298 - 862 0.238 - 1012 0.333 - 1022 0.453 - 1138 0.281 - 1153 0.244 - 1160 0.443 - 1455 0.272 - 1603 0.149 - 1632 0.780 Yes 1645 0.367 - 1653 0.236 - 1780 0.357 - 1840 0.363 - 2047 0.226 - 2058 0.456 - 2089 0.358 - 2131 0.771 Yes 2161 0.243 - 2197 0.618 Yes 2233 0.450 - 2236 0.532 Yes 2272 0.599 Yes 2308 0.415 - 2369 0.263 - 2467 0.640 Yes 2689 0.255 - 2731 0.121 - 2827 0.291 - 2926 0.285 - 2970 0.070 -

Explain the output -- Processing locus 42: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGTGTTTTTGCTTCTTAGTAGTATCTATTTGCAAGGAAACTAGGATTGATTTCCCTTTGCTGTTTGATTTAGATGATGT TCACAGTTTTTATGTTCTTGAAAGGTTACATTTTTTCAGCAGTTTGAGATTCCTATGATGTTGAAAATGCAGTTCTTTTG TGCTTGTTCAGGTTTCACTACGTTGAGGATGGTTCTTCTGATGGGTGCCGAGAATAATCTTATGGAAGAAACAACAGCTG TTACCGAAGAAGATTCCTATGATGATGAGTTTCCCTGCGGAGATCCACAAGTTGAGCCTCGTGTTGGAGATGAATTTCAG GTCGACATTCCTCCTATGATGTCTGCAACCAAGCGTGCTGTGTTTCTTTCGACTCCTGTTGCTTTGGATGATTCGTCATA TTCTTTTCTCATCGGACTACCTGTCCAAGTCATGTGGATAGACAAACATAGAAGAGGGCAAGGAAATGGAGATGACAATG TTGACATGAACCAGTCCTTGAAGTCTTTAAGAGCCAAAAAAAGCCGTTGCTCTGCCAAGATCAGAGGAAAGAGTGACAAG AATTCGGAAACCAAGAAGCAAAGATCGAATCTTGAGGCTGTTCCTGTGATACCGTCCAGTTCTTGGGAAGATTTGGAGGT GGCTAGCTTTGTTCTTGGTCTGTATACATTTGGGAAGAACTTTACTCAGGTGAAGAATTTCATGGAGAACAAAGGAATAG GAGAGATCATGTTGTTTTACTATGGGAAGTTCTACAATTCTGCAAAGTACCACAGTTGGTCTGAATCCCGAAAGAAGCGC AACCGCAAATGTGTATTTGGAAGAACGCTCTATTCAGGTTGGAGGCAACAACAATTGTTAACCCGTTTGATGCCTTCTAT TCCTGATGAACCTCAGAAACAGATACTTGTGGATGTCTCAAAGTCATTTGCTGAAGGTACTATCACTCTAGAGAAATACG TAAGTGCAGTGAAGAATCTTGTGGGTCTCAGGCTTCTGGTAGACGCTGTGGCGATTGGTAAAGAAAAAGAAGATCTCACG GTTCCTACATCAACACCAATGAAGACCAAACCATGGTTCACTGTTTCTTCAAAATCTTCTTTGGTCCCTGGAGAAGGGGA TTACAATTCCCTCACATCGGCTGGCATAATAAATCAGTTAACTGGCTGTTCTCGTCTGAGCAAAGCCCGTTGCAATGATA TATTCTGGGGCGCTGTATGGCCGCGTTTGCTAGCCAGAGGATGGCATTCACAGCAGCCAGAGGACCGTGGCTATTTCAAA TCTAAGGATTACATTGTTTTCATTGTCCCTGGCGTGAAAAAGTTTTCAAGACAGGAGCTTGTCAAAGGAGATCACTACTT TGATTCCGTCAGTGATATTCTAACAAAGGTTGTGTCAGAGCCTGAACTTCTTGAGAATGAAACAGGAGGAGTTGCTGCAG AGCTTTCTTCTGACAAGTCAGATGAAGAGTCTGTCCCGTCTGATAGTCTAAGACACCGTTACCTCAGGTCTCCATGTTCT AACCGTGGAACTCTGGGAATGAAATTCACTGTTGTGGACACTAGTTTGGCTACTGGAGGAAAGTTGTGTGATTTACGGAA TCTGAATGCAGAGTGTCTAGTAGTTTCTGAGCCAAACGTTCGTCTAGAAGTTAAAGATTCTCCTGTGCTTAAAAATTCTC TGGACAGTCAGAATGTGGAAAAGTCGCAAGTGAGGCCTCTGGATGCCAAGAACCAAGTGGATGACCCAATGCGATTCACG ATTATTGATACGAGTGTAGACCACTGCGAAAAATCATCTGGGTTTAGGAGATGGAGATATTTACCAAGTGATGAAACAAA AAGGGGTCATGTAGGGGCTGATTCTGGTATAAAGGAAGAGAAGACTTTGGAGAAGGTTAAGGATCCATCAAAGAGGGTTA TTAAGCATCGGTCTACCCCTCGAGCAGAAACTAATTATCATGCTGTAAATTCTGCACCATATTTGAAACGCAGACGGCTC AGTGCTTGCATTTCCAGAGAAAGTCCAGTGTCCAAACATATACCAGGTGATGATGACACAAAAATGACTGTTTGTCTTGA ATCAGAGCAGCAAAGTATATGTGTGGTCCAACAACAAACTAGCACTTGTGAAGAGATGAATCAGGATAAAGAGACTGTGC CGTTGGTTGAGCATATGAAATTAAAGTCTGATCAATCAAAAAAGACTGGAACTGGGCCATCATCTTTGCTTGTTGAGATT CAAGAAACGACAGAGATTGAACCAAGCGGGTTAAATTCCAACACTGGTGTAGACAAGAACTGTTCTCCAGAGAAAATAAG TACATCCCATGAACAAAAACCAAATGGGCTTTGTTCAGTGTCAAAGTCGGACAAAAAACGAGCTTCCAATGATCTGGAAC AAAAGCAAGCACTTGAGCTTCCTTCCATTTCAGGCTTAAACAATAGATCTTCTTCCAATGATCTGGGAACTACTCAAGAG CTGGGTTCATCTGAACAACAACACGGCCAACAGAATAATACAGATGGTCCTAGAAGACAGAGCACAAGAAAGAGACCATT GACCACTCGGGCTCTGGAAGCTATGGAATCCGACTTTCTTACAACCAAGAGAATGAAAAGCACGACTAAACCAGAACCGA GAAAGCGTGAAAGTTCAACGAAGAAAAAACGCTCAGCTAAAGCGTGTAACAGAAATGGGAGTGCAGACTTGGAGCACAGA AGAGAAGATAGAAACATTTTTATATGGAAAGCACCAACAAGTAAGCCCTTGGATCAAATAGAGGATACAAAGCCTCGCTA TCTTCTCAATGAAGCAAGAACTGAGAGTAAGACTCTAGATAGAATACAGGAGTCAAGGCCGGTCCTAACTGAATACCGTA AGCTTCCCCCGATTGTATTGAAGCTTCCGTTCAGGCGTGGCTAAGGAGAAGATTGGACTTAAGTCTGAAAGTTTTGATCG CTGGATGTGAGAAGATTGGTCTTCCTGAAGCATAAATGTGTACAGAAGAGAAATGTTTTACAC .........................................................................N..N... ...........N..........................................i..N........i............. ............................i...........i....................i.................. ..................N..N..N............................................N.......... ...............N..i................................................N............ ...............................N.................................i.....i.....N.. .....N.......................................................................... ................................................................................ .............................................................N.................. ........i............i.......................................................... ........N............................................................i.......... .....N..........................N............................................... ................................................................................ ..................N.............N............................................... ..........................................................................i..... ................N.......................N....................................... ................................................................................ ........................................................N....................... .....................N...................................................N...... ..................i............................................................. .....N.......................................................................... ............N.............................N.................N.......N........... ..................................................N...................N......... ........N....................................................................... .......................................N........................................ .................................................N..N..........i................ ..................i....................................i........................ ..............N................................................................. ................................................................................ ........N..............N............................................N........... .........................................................i...................... ...........................................N.................................... ......................i.............................N........................... ......................................................i......................... .......................N........................................................ ........N....................................................................... ................................................................................ ....N..............................N................N.......... Pos Score Pred ------------------------ 74 0.237 - 77 0.168 - 92 0.437 - 135 0.616 Yes 138 0.470 - 147 0.835 Yes 189 0.806 Yes 201 0.834 Yes 222 0.520 Yes 259 0.130 - 262 0.145 - 265 0.093 - 310 0.047 - 336 0.439 - 339 0.577 Yes 388 0.220 - 432 0.444 - 466 0.853 Yes 472 0.587 Yes 478 0.444 - 486 0.303 - 702 0.344 - 729 0.610 Yes 742 0.612 Yes 809 0.455 - 870 0.577 Yes 886 0.105 - 913 0.162 - 1059 0.322 - 1073 0.304 - 1195 0.566 Yes 1217 0.286 - 1241 0.317 - 1417 0.194 - 1462 0.313 - 1514 0.309 - 1539 0.657 Yes 1606 0.324 - 1693 0.229 - 1723 0.457 - 1741 0.196 - 1749 0.436 - 1811 0.225 - 1831 0.232 - 1849 0.176 - 1960 0.274 - 2050 0.351 - 2053 0.370 - 2064 0.753 Yes 2099 0.661 Yes 2136 0.667 Yes 2175 0.313 - 2329 0.160 - 2344 0.304 - 2389 0.350 - 2458 0.565 Yes 2524 0.224 - 2583 0.541 Yes 2613 0.461 - 2695 0.528 Yes 2744 0.277 - 2809 0.135 - 2965 0.045 - 2996 0.053 - 3013 0.082 -

Explain the output -- Processing locus 43: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAATTTGCTTAATTTGGTGTCTTTGATTTTGAGTTGGATTGGTTTTGTAATAGAAAGCTCCTGTTTTATCCATTGAATT GTAATACATTCTGGCATTTGAGACTGGGTTGGGTCCCTTATGTTTGTTTCTGGAGGTGGTAAAACTAGAAAGTTAAAAAG TCTCATTCTTTGTAATTTCTTGTCAGCTGGAACAATGCCATCTGATATATTGGAACCGAGAGGTGTGCCTACACCTTCTC ATTTTCATGAAGACATTCGCATCACTCCTGAGAAACAATTTGGTTTCATGAAAAACAATCCAATGCCTGAAGGTGGTGTG GATAGAAGTTCAAATCTACCGACTAGTTCTTGGACATCTGACAGTTACCAGCTCTCTCAACAATCAAGCTTGTCTGGGGC GCTTCCCTCCTTTATCCCTAATGGAAGAACAACTACAAATGATACTCACTGGGAGAGCAGTCTATTCTCAAGCTCTCTGT CTGACCTATTTAGTAGAAAATTACGACTACCAAGAAGTGATAAGTTAGCCTTTATGTCTGCAAACCGCGAGGAAGAACCT TCAGAGTCCTTAGAAGAAATGGAGGCGCAAACTATTGGAAATCTTCTTCCTGATGAGGATGATCTTTTTGCAGAAGTGGT TGGTGAGGGTGTCCATAAGTCTCGTGCCAATGGTGGAGATGATTTGGATGATTGTGACCTATTCAGCAGTGTTGGTGGCA TGGAACTAGATGGAGATGTTTTCTCCTCTGTCAGTCAAAGAGATGGCAAGAGAGGCAGCAATGTTTCGACTGTTGCTGAG CATCCTCAAGGAGAAATTTTATCCAGAATACTTTTTGTTAGGAATGTGGATAGCAGCATTGAAGATTGCGAGCTGGGGGT CCTTTTTAAGCAATTTGGAGATGTTCGGGCTCTTCATACAGCTGGCAAGAATCGTGGCTTTATCATGGTATCATACTATG ATATAAGAGCTGCTCAGAAAGCGGCAAGAGCACTCCACGGTAGGCTCTTAAGGGGAAGGAAGCTTGATATTCGCTATTCT ATTCCAAAGGAAAATCCTAAAGAGAACTCCAGCGAAGGAGCCTTGTGGGTAAATAATCTTGATTCTTCTATATCCAATGA AGAGCTTCACGGTATATTCAGTTCATATGGAGAAATTAGAGAGGTTCGTAGAACGATGCATGAGAACTCACAAGTTTACA TAGAGTTCTTTGATGTTCGAAAGGCAAAGGTTGCACTTCAAGGACTAAATGGACTTGAGGTCGCTGGGAGGCAGCTTAAA CTTGCACCAACCTGTCCAGAGGGTACAAGTTTCTGGCCACAGTTCGCTTCAGATGATGGTGAGGGGGGTCTACCTAAAAT GGCTTTTAATAACCTATCATCAGCTCACATGGGGAGACATTTCCCAGGAATATTAGCTTCAACCTCCATTGATGGTGGAT CTATTCGAGGTATGCACAATTCTGTTGGGTCACCTATGAACTCCTTCATTGAACGTCATCAGAGTCTCGATGTTCCTATT GGTTTGCCACCATCGGCAAGAGTCATCTCAGCTAGCAAGCCTGTTGGTCTTCAGGAGTTTGGAAACCCATTTGATAATTC GAAAACGGGGATCCAAAGTATGCCAAATCTCCATCCTCATTTTCCTGATTACCTTGACAACTTTGCAAGCGGTAGTCCAT ATAAATCCTCTACGACATTCTCTGAAATGGTAAGCGATGGCCAAAAAGCAAATGAGGGCTTTATGATGAGCAATGTTCGT GGAGTGGGAGTAGATGGATTTAATGGAGGTGTCATAGGATCTCCCATAAATCAAGGATCCCATCGTGGTAACCTCAACTT ATGGAGTAACTCTAACAGTCAGCAACATAATCAGTCAAGTGGCATGATGTGGCCTAACTCACCATCTCGCGTCAACGGCG TTCCCTCTCAGCGCATCCCACCTGTTACTGCATTCTCTAGAGCATCTCCTTTAATGGTGAACATGGCATCATCGCCTGTG CACCACCACATTGGATCTGCCCCGGTATTAAACTCACCTTTTTGGGATAGAAGACAGGCATATGTAGCTGAATCTCCAGA ATCTTCAGGATTTCACTTAGGTTCTCCCGGTAGCATGGGATTTCCTGGCTCGTCTCCCTCACATCCAATGGATTTTGGTT CTCATAAGGTTTTTTCTCATGTCGGTGGGAATCGCATGGAGGCCAATTCAAAAAATGCTGTATTACGATCATCGCGACAG ATGCCTCACCTCTTTACTGGAAGGAGCCCAATGCTTTCAGTGTCAGGCTCATTTGACTTGCCTAATGAACGATACAGGAA TCTCTCTCACCGAAGAAGTGAATCTAACTCTAGTAATGCTGAAAAGAAACTGTATGAGCTTGATGTAGATCGCATATTAC GTGGGGAAGACAGTAGAACAACGTTGATGATAAAGAACATTCCTAATAAGTATACTTCTAAGATGCTCCTAGCTGCTATT GATGAGTACTGTAAAGGAACATATGATTTTCTTTATTTGCCAATTGACTTCAAGAATAAATGCAATGTCGGATATGCTTT CATCAACCTTATCGAACCTGAAAATATTGTTCCGTTTTATAAGGCATTCAACGGTAAGAAATGGGAAAAGTTCAACAGCG AGAAGGTGGCATCGCTTGCATATGGTCGGATTCAGGGGAAATCAGCTCTTATTGCCCATTTCCAGAACTCAAGTTTGATG AACGAAGACAAACGTTGCAGGCCTATTCTCTTCCACACTGCTGGTCCAAATGCTGGTGATCAGGAACCATTTCCAATGGG TTCAAACATCCGGTCAAGACCAGGCAAGCATCGAACAAATAGCATCGAGAACTACACCAACTTTAGCAGTAGCTCTGATA ACCGAGACGAACCTGCAAATGGGAACGACTCCATGTAACCGATAAGCCAGACAGTTTTCATGTCGTAAGCCAGACTAACC CTTTTAGGCGTCTTGTATCAGAGGTAAAAGAAAAGAGACCCAAAAGATTAACACAAG ................................................................................ .......................................N........................................ ..................................i............................................. ......N........................................N..............i................. ................................................................................ ....................N.................N......................................... .....................................................i.......................... ..................i.................................N.....N..................... .............................N........i........N...............................i .........N.....N..........................N.................N................... ...........................................N.................................... ....................N...........................................i............N.. ................................................................................ ............................................................................N... ..........................N............................N...N.................... ............N...................................N............................... ....................................................N..N......................i. ............................N..........................................i........ ...........i.......................N.................................N.......... ................................................................................ ...................i............................................................ ..........................i.........i..............N..........N..N......i....... .............N........N......................................................... N..........................................i..i................................. .....................................................i........i................. .............................................................N.................. ..................................i................................i............ ..................N................N..................i......................... i.............................N.................................N............... ...................................N.................N........N................. ..........................N...................................N................. ............................................................i................... .....................N.......................................................N.. .................................................i.........................i.... ................................................................................ ..................i.............N..........................N.................... ......................................................... Pos Score Pred ------------------------ 120 0.293 - 195 0.823 Yes 247 0.125 - 288 0.255 - 303 0.567 Yes 421 0.489 - 439 0.493 - 534 0.664 Yes 579 0.855 Yes 613 0.191 - 619 0.096 - 670 0.384 - 679 0.521 Yes 688 0.125 - 720 0.690 Yes 730 0.255 - 736 0.127 - 763 0.389 - 781 0.232 - 844 0.389 - 901 0.336 - 945 0.605 Yes 958 0.147 - 1117 0.179 - 1147 0.218 - 1176 0.417 - 1180 0.320 - 1213 0.159 - 1249 0.372 - 1333 0.159 - 1336 0.230 - 1359 0.707 Yes 1389 0.490 - 1432 0.632 Yes 1452 0.512 Yes 1476 0.302 - 1510 0.355 - 1620 0.743 Yes 1707 0.729 Yes 1717 0.693 Yes 1732 0.480 - 1743 0.425 - 1746 0.423 - 1753 0.604 Yes 1774 0.412 - 1783 0.446 - 1841 0.434 - 1884 0.640 Yes 1887 0.725 Yes 1974 0.507 Yes 1983 0.624 Yes 2062 0.396 - 2115 0.669 Yes 2148 0.660 Yes 2179 0.389 - 2196 0.427 - 2215 0.566 Yes 2241 0.628 Yes 2271 0.335 - 2305 0.495 - 2356 0.204 - 2374 0.218 - 2383 0.077 - 2427 0.413 - 2463 0.347 - 2482 0.189 - 2503 0.111 - 2540 0.383 - 2545 0.641 Yes 2554 0.109 - 2621 0.577 Yes 2662 0.445 - 2718 0.482 - 2770 0.584 Yes 2796 0.854 Yes 2899 0.520 Yes 2913 0.490 - 2940 0.096 -

Explain the output -- Processing locus 44: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAACTAGACTTGAGCTAGTGCTATTAATACCGGTTTAGTTGAGATGGACTGGTTTGATCCGGTTATTAAAAATTGGGA ATCCAGATTACTTGGGTTTAAAAAGCAGAAAGAAGTTTGAGTTTTTTGATTCACCGATTGCAGAAAACAATGGCACTGAG CTCACTCGTTGAATTCGTGATTCTCGTCGTGAAGAACCCATGTTATCAACAAGACGACGCATCTTCTTCGATTCAAGAGA TTTCGATCTCAGCTTCTGAAATTGCTTCCTGGGATATGTCAGAGATACTCTCTTACGGAAGTGTAAAAGTTCGAGCTCAT CGAACTAGGCTTATTCAGGAATCCTCGTATTTCCATGGCCTTCTAAGCGGAAGCTTCAGTGAATCAGGCCTTGATCATAT CTCTGTTGAATGGAACCTAGAGAGTTTCTTGAATCTTCTCATGTGTTTGTATGGTTACGATATAGAGATTACATCCAGTA GTTTCTTACCTCTATTTGAGTCTGCACTCTACTTTGGGGTGGAGAAGCTTCTCTCTATATGCAAAAACTGGCTTTCAGTG TTGGCATCATCGAATGACAACGCATTACCGAAAGTAGAATTATCTGACCTTATTCAGATTTGGAGTTTTGGTCTCGAGCA TGCTGGAGAATTTGTGCCAGATCTCTGTGTGGCCTATCTTGCAAAGAATTTCATGTTGGTGAAATCTGATAAGTATTTTG GAAATGTTCCATATGAGTTGTTGATGTGGTGCGTTAAACATCCTCACCTGACTGTACACAGTGAGATGGACCTTGTTGAT GGACTTCTTATTTGGCTTGATGCCGGTGGAAGATTGTCTGACTTACCAGAGAGTAGTCAAGATAACACAATCAACTTGAT GGAACAGGTCCGTTTTAGTCTTTTGCCGCTGTGGTTTATTGCAGGAAGAAGCAAGTCTCATGGTTTCTCAAAGTTTGCTG ATCAAAGCATTGAATTGGTGACTAAACTAATGAAAATGCCATCCACATGCTTGGTCGATAGTCTAACCGATGGCCCTCCA ACTGATGTTAGAGTTCGGCTGACTGAATACAGTGAGATATTGGATCTCTCAGGATGTCCACAGTTAAATGAAGCCAGCCT GCTACTGTCAATACTGCCTAACTCGTATTTCGCAAACCTCAGGTGGAGGAAGAGTTTGGAAAGTTTCCTAAAGAACCCTG ATGACGATGAAAGACATCAAGAACAAATATCTCACAGAACTTTACCAATTTTGTCTTTTGAATCGGTGAAAGAGATTGAT ATTTCCAAGTGTCAGAGGCTTGATTATAAAGTTGTGATCAAGTGCTTCTCTAAGTCATTCCCATCATTAAGAAAACTAAG AGCTGCTTATCTCTTAAACATCAAGGTGTCTACCCTGCTTGAACTTCTCCTGAATTTTCGCGAGCTCACTGAAGTTGATT TAACAGTTGATGTCTCTCCTATAATACCGGTTCAAGCATCTGTGTTTTACTCTGGCCAAGGTCATTGTTTGCTATCGAGT ATCACAAGATTAACGCTGGAGGGTAGAAGTGACATATGTGATATGGAACTACGGAGCATCTCTAGAGTATGTGAGTCGTT ATGTTACCTGAACATAAAAGGGTGTGCACTACTCTCAGATGCATGTATAGCATCTGTTATACAGAGGTGTAAAAAATTAT GCTCTCTCATAGTTTGCTATACCTCTTTCTCGGAAAATTCGATTTTGGCACTATGCGCTACTATATCTATGACCAATGAG CATATGGATATAAATTCAGTAGCATCCAATCTTCAAACTCTGCACATGAGTAAATGCGAGGGAATTAGTGAGACGTCGCT TCTTAACTTGATAACTCATTCACAGAAAATGAAAAGTCTTTGCTTAAGGGATACAAAAGTTTCAGACAGTGTGCTATGTG AATTCCCCGGCTCTACCTTGGAAGCACTTGATATTTCAAATACCACGATATCTTGGATGGCTCTTGCTCGTGTAATTAGC AGAAATCCTAATCTAAAAACCCTCAAAGCCAGGGGATGTAAAAATCTACTCCAATTAGAAGTTGACGGCAGAACTGACAA TTTCTCTCCGTTGGTTTCTGGTCAAGAGGTGTTTAAATGTTTAAGCAAGGGGAGTGGATTGGAGGAGCTTGAGATTGGTT GGGGGTTTTCTTATTTCTCTTTTGAGTCTTTGAGACCTGCAGCTTCGTTCCTACGAGTTATAAGTGTGGGTTTGGGTGCT TCCTTAGGTGAAGATGTTTTAAAGCTTCTACCTTCAACTTGTCCTTTGCTAGAGTCTATCGTCCTCCATTTTCAGGAAAT ATCTGATTCTGCCTTGACAAGTGTCCTGACATCGTTGAAGCACCTGCAAGAGTTAGCTTTGTCCTATTGCTTCGGTGAAA TATCCCTTCAAAGTTTCAAGTTCTCTATGCCGAACCTGAGAAAACTAAGGCTTGAGAGGGTAACTCGTTGGATGACCAAT GATGATTTACTTGTTCTCACACAAAGCTGTCCGAACTTGACCGAGCTTTCATTAGTAGGATGTTTACACTTAACTTCAGA TTGTCAACCTATCATCTCAGCTGGATGGCCAGGAATGATTTCTCTTCATCTAGAGGAATGTGGAAGTATAACAGAGAATG GTGTCGCTTCTCTCTATGGTTGCATTGCTCTTGAAGACCTTTTTCTTCGCCACAACGGCTCTGGAATACAGAAAAGCTTC CTTCTTGATGCTACTTTGAAGTTTCCGATGCTCCGGCTGGTATCTCTGGACATGTGTGATGCAAAGGAAGGCGGTTTTGA TGTCCCAGAGGAGAAAGAAGAAGGTCGTAGTCTAAGCATAGTCAAGATCAGTAGATGCAAGTCTGATAGATGCTCGCTGG GAAGACGTGCGGCTCCAATGCATAGAGAGACGCTGGTTATGTTATGGAATGGCCAAACCCTCACTAAAACACTGCTCAAA CAAAGACTTTAATCACCAACACTCTAGACTTTATGTTTAGCCGAAAGATTTTTGTTTCGAAATTTAAAATTGAAGTTTGG TCGGTGTAGTTGTAGATTAAATTGTATGCAA .............................................i.................................. .....................................................................i.......... .......................................N........................................ ...................................N............................................ ..................................N............................................. .........i..............................N.........N............................. ..........................................................N..................... .............i.................................................................N ....................................................N........................... ...N........N..........N.........................................i............i. ...................N..........................................................i. ...........................................................N.................... .............................i.....i..........N......................N.......... ....N................................................i.............N............ ................................................................................ N.....N......................................................................... ................................................................................ ................................................................................ .........N...................................................................... ...................................i......N.........................N........... N.....................................N...N...................................N. ....................................................N...............N......N.... ...i.........................................N.......i.......................... ............................i..............................................N.... ........................................................N....................... ...................................N............................................ ....................................N........................................... ................................................................................ .............N.................................................................. ................................................................................ ..........................N............................................N......N. ........................N.........i......................N...................N.. ...............N................................................................ .......N...................i.......................N......i....................N ......................................................N..............N.......... .................N....................N....N....N............................... ................................N............................................... .........................N..... Pos Score Pred ------------------------ 46 0.647 Yes 150 0.633 Yes 200 0.482 - 276 0.482 - 355 0.143 - 410 0.557 Yes 441 0.280 - 451 0.425 - 539 0.293 - 574 0.577 Yes 640 0.301 - 693 0.335 - 724 0.355 - 733 0.348 - 744 0.428 - 786 0.766 Yes 799 0.515 Yes 820 0.183 - 879 0.602 Yes 940 0.401 - 990 0.660 Yes 996 0.637 Yes 1007 0.375 - 1030 0.489 - 1045 0.335 - 1094 0.764 Yes 1108 0.336 - 1201 0.494 - 1207 0.191 - 1450 0.270 - 1556 0.503 Yes 1563 0.227 - 1589 0.336 - 1601 0.125 - 1639 0.273 - 1643 0.123 - 1679 0.371 - 1733 0.268 - 1749 0.361 - 1756 0.095 - 1764 0.598 Yes 1806 0.430 - 1814 0.546 Yes 1869 0.651 Yes 1916 0.483 - 1977 0.467 - 2036 0.282 - 2117 0.458 - 2254 0.107 - 2427 0.271 - 2472 0.280 - 2479 0.318 - 2482 0.420 - 2540 0.321 - 2585 0.117 - 2595 0.674 Yes 2618 0.240 - 2638 0.483 - 2656 0.108 - 2728 0.214 - 2748 0.809 Yes 2772 0.439 - 2779 0.550 Yes 2800 0.260 - 2855 0.491 - 2870 0.490 - 2898 0.135 - 2919 0.171 - 2924 0.136 - 2929 0.291 - 2993 0.088 - 3066 0.030 -

Explain the output -- Processing locus 45: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCATTTTATTGAATTGATTGATTGATCAAATCATATAAGATTATTATGTTCGTCGGTGACTTTCTGGATTTGTTAATTGC TGTTTTTACTACTTAAGTTGATTTGAATCTATGGCTAATAGAGCTTTGAATTGTGTTTTTGTTTCTGGAGGAATGGGAGA AACAGTTCATTGTGGAAGCTTCTTGTCCTCAATGAGGGATCTAAGTGAAGATATTTCCAATACTTGTAGCTATTCAATGT ATTGTGGAGGTGATAAAACTTTGCCTTATGGGCAGTATCAGAATGGTTTCTCGGCGAGACCTCCAACGGATTCTTACGAG AGAGATTTTTTGAAACAAACAATGCTTGAACATGAAGCTGTATTTAAGAATCAGGTTTATGAGCTTCACCGTTTGTATAG AACACAGAAGAGTTTGATGGCTGAAGTTAAGGGAAAGAACTTTGTTGATCACTTGAACAACAATGAGCCTACACCGGGAA GTGGGATTAAACGAGGATTTCTTTTCGGAAACTCGATATGTGGCGAAGGAAGCACCTCTCAAGATTGCAATGTTGGCAAG GACAACAAGGTGTTAGAGGTTAGACCTGTCAAGGTTAGGAGAACAATGATTGATCTTCAACTTCCCGCAGATGAGTATCT CCATACAGAGGGTGATAATACGACTTGTCCTCCTTATGAGCAGTCAAAAGAAGTTGGTGAAAACATCTTCTTTGAATCTC ATCGGAACGATTCTTCTGGATCTTCTTTGCTTATGAAGAACTCAAATGGATTTACTGACTTGAACGAGCCAGTCCAATGC CAAGATTCAGTACCTGTTTCTTCTTCTTCCAGGGACTTATATTCTCTTTATGGAGCAAACATTTCACACGTGCAAGGCCA GTGGGTGGAAAAAAATACTAGTCAAAATGGATGGATGGTTCTAGAAGCAGGGAACGGAAAAAGCACTCCAAGAGACAAGT TATGCCTGCCTTCCCATTCGGTACAAGTTCTTTCTAACAGTGCATTTCAACCTCTGGGATACCCTTCAACTGACCACAGC AAGTTATCGGGCGAAAGAGCATCTTTCAAATGTGAAGTTCGTCAAAGGAATCCTGAGGTTTCGTATGATAGCTATGTGGA ATCAAGTGTGGCATCAAATGTTCCAAGCTTGAATCATGGATATCGCCCGGAATCTGTCAGACCTTGGAGCCATTGGATTT CATCATGGGAGAACCGAAGTAGCAGCTCAGTTCAGAAACCTTTGCCACTTCAAGCGAATCCATTCTTGACTTTTAATACT CAAGTACGAGCAGACTCAAGTGCAGAGATGAGAAGCCGTGATTCTAACGGGCTTAACCAAGGATTCTCTTCATTCTCGGA GGAAAGTGCCTTTAATTTTCCATCAGTAAACTTTAACCATCTTAACAATGGCCCAAAAGGGGCAGTTACTAATGGTTCTT TGTGTGAGAGTGTGATGCATCAGAGTCTTAAAAACCTTCAAGGACCAAAAAAGCAGGAGTGCTCTTCTGGTTTGCCCTGG ATAAAACCTAAGCCCCTTAACAAAAATGGAAAAACCAATGGTGGTTTGGATTTGAATGCTTCTGCAAATCATCAATTCAT GGATGAACGTGATATGGGTGACAGCTCAAATTATGTACATCCTCAGAATGGTTTGAGGTCTGTTACATGTTCTAATGACG CCAACTTGAGACATGTTGAAATGGCCAATTCACAGAGTCGTAGAAAGATTCTTGGTTTTCCTATCTCTCAGAAGCTTTCT ATTTGCGAGGAGCACCCTTCCCTTATAACTTCTTCTGTGTGTATCTCTAATGAACCCAAGAAAGTAAACAACTTGGTGAA GATAAACCTCGATATCAACCTGCCATGTGAAGCCTCGGTTTCTGAAGGAGTTGTTGTGGACAAGGAAGAAGGTAATAAAG CTGCCACTCACAGGCAGCACATTGACTTGAATTTCTGTGCTAGCGAGGATGAAGATTCCGGCTTCTGTTCTAATCCAAGA GTGGAAACAAAAGCAACTACTTTGATAAATGTGGAAGCTCCCTTGACGCTTGAGAGTGAAGAAGAAGGAGGGAAATTTCC AGAGAAGAGAGATGAAGCTGGAGACTCTGTGGATGAACTCATCGAGGCAGCAGCAGAAGCTATAGTCACTATCTCACTGT CTTATCATTGCCGCAATACTGATGAAGCTGCTTCCTCTTCGACTGATGCAGTTGACAAGGAGCCGCTCTCTTGGTTTGTG AACACAATAGCTTCTTGTGGCAATGATTTAGAGAGCAAAATTGATGCTTGTTTGGAAGCCAGAGACTGTGAAGGCTGCCG TGAAGAATGTTCTTCAGGTGAGTTTGATTATTTTGAGGCAATGACCCTGAACTTAACACAGACCAAAGAAGAAGACTACA TGCCAAAGCCTTTAATCCCTGAATATCTGAAATTTGATGGGACGGGTTCTATGGGTATCACAAGTAATAGGCCAAGAAGA GGACAAGCGAGACGAGGAAGACCAAAGCGGGATTTCCAAAGGGATATTCTCCCTGGGCTAGCTTCTCTGTCTAGGCTCGA AGTAACCGAAGATCTTCAGATGTTTGGGGGACTTATGAAAGCCACAGGCTACAACTGGAACTCAGGAATGGCCCGAAGGA GCTCAAACCGAGGAAGAAAACGACTGGTCAGCAACATTGACAGAGCTCCGGTTTGCTCCTCTTTGGCACAGCCAATGAAT AACAGTAGTGTACAAATGGTGGGACTGGAAGATAGGAGCCTTACAGGGTGGGGAAATGCAACCAGAAGACCAAGGCGACA TAGGTGCCCTGCAGGTACTCCCCCAACAGTGCTATTAACATAAAGCTTTTCGAAATAAATCCGATTTCACAATTTGAGGA ACATTATAGAGGGAAAAGAACCAGAGCAAGTAACAATGGACAAAAACTGATTTTCAGCGATC .............................................N.................................. ..............................i.........................................i....... ...............................N............................................i... ...........................N..............N..................................... .....................i.........N..........................N..................... ................N.............................................i................. .....................................N...............................N.......... .............................................i........................N......... ...................................N............................................ ................................N............N..............................N... .................................................N.............................. ..........................N...i...N............................................. .............................N..................................N........N...... .................i.................i............................................ ....N........................................................................... ...........................N.................................................... ...............................................i.......................i........ ..............N................................................................. .........................N...........N.................N......................i. ..N..........i..................N..............N..................N.......N..... ............N.......i........................................................... .................................................N.............................. ........................N....................................................... ................................................N............................... ............................i................................................... ...........N....................N............................................... .....................N.......................N.................................. ......................N....................N.................................... ......N.................................N......................................N ....................................i.............N............................. ................................................................................ ...................N..............N................................i............ ..........................................................................N..... ...............N.......................................N........................ ................................................................................ ...................................N.......................... Pos Score Pred ------------------------ 46 0.409 - 111 0.749 Yes 153 0.662 Yes 192 0.308 - 237 0.735 Yes 268 0.206 - 283 0.340 - 342 0.738 Yes 352 0.203 - 379 0.183 - 417 0.399 - 463 0.538 Yes 518 0.425 - 550 0.192 - 606 0.755 Yes 631 0.218 - 676 0.135 - 753 0.469 - 766 0.453 - 797 0.344 - 850 0.434 - 907 0.366 - 911 0.688 Yes 915 0.446 - 962 0.343 - 1070 0.218 - 1105 0.204 - 1114 0.465 - 1138 0.686 Yes 1156 0.675 Yes 1205 0.465 - 1308 0.430 - 1408 0.532 Yes 1432 0.538 Yes 1455 0.344 - 1546 0.494 - 1558 0.297 - 1576 0.128 - 1599 0.627 Yes 1603 0.238 - 1614 0.835 Yes 1633 0.235 - 1648 0.300 - 1667 0.183 - 1675 0.407 - 1693 0.172 - 1701 0.600 Yes 1810 0.227 - 1865 0.253 - 1969 0.191 - 2029 0.537 Yes 2092 0.281 - 2113 0.090 - 2182 0.255 - 2206 0.176 - 2263 0.382 - 2284 0.310 - 2327 0.212 - 2361 0.466 - 2400 0.370 - 2437 0.530 Yes 2451 0.439 - 2580 0.444 - 2595 0.461 - 2628 0.616 Yes 2715 0.304 - 2736 0.312 - 2776 0.242 - 2916 0.093 -

Explain the output -- Processing locus 46: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTACTATGTCTATAAAGAGATGGGCACGAAACCATCTTATTTACTACCACAAACTCCACTTTCTTTTATCAATATTCAAG AAAATTCGACTAGCTAGCTAGCGTAAATTAAGGAGGATGAAAGGCTCTTGGGTGGTCTCAACGAGTATCATTCGTATTAC TCTTTCTTTTACTTTCTTATTCATTTGTCATTTTTCGGACGTGCTTGCGGCTCCTACTAGGCACTTGTGTCGTCCCGAAC AAAAGGATGCACTTCTCAAGTTCAAAAACGAGTTTGAGATTGGGAAGCCTTCTCCTACTTGTAAGATGGTTGGCATTGAA TCTCATCGGAAGACGGAGTCATGGGGGAATAACAGCGACTGTTGTAATTGGGAGGGAGTCACGTGCAATGCCAAGTCCGG GGAAGTGATCGAGCTCAACCTTAGCTGCAGCTCCCTCCATGGCCGGTTTCATTCCAACAGCAGTATTCGAAACCTTCATT TCCTAACCACTCTAGACCGTTCACATAATGATTTTGAAGGTCAAATCACGTCTTCAATTGAAAATCTGTCTCATCTCACC TCTCTCGACCTTTCTTATAATCGTTTTTCGGGTCAGATTCTGAATTCAATCGGAAACCTTTCTCGTCTCACCTCTCTCGA CCTTTCTTTTAATCAGTTTTCGGGTCAGATTCCATCATCAATTGGTAATCTTTCTCATCTCACCTTTCTAGGTCTTTCTG GTAATCGTTTTTTTGGTCAGATTCCATCTTCAATTGGTAATCTTTCTCATCTCACCTTTCTAGGTCTTTCTGGTAATCGT TTTTTTGGTCAGTTTCCGTCTTCAATTGGAGGTCTTTCTAATCTCACCAATCTCCACCTTTCTTATAACAAATATTCGGG TCAGATTCCATCTTCGATTGGCAACCTTTCTCAACTTATCGTTCTCTACCTTTCTGTTAACAATTTCTATGGTGAAATCC CATCTTCTTTTGGCAATCTAAACCAGCTGACCCGCTTAGATGTTTCTTTCAACAAGCTCGGTGGAAACTTTCCCAATGTC CTACTAAACTTGACAGGGTTGTCAGTTGTATCACTCTCCAACAATAAGTTCACAGGCACGCTTCCTCCTAATATCACTTC ACTATCCAACTTGATGGCTTTTTATGCAAGTGACAACGCCTTCACCGGAACATTCCCTTCTTTTCTCTTCATCATTCCTT CTTTGACTTACCTTGGTTTGAGTGGTAACCAACTCAAAGGCACTCTTGAGTTTGGGAATATATCTTCACCATCTAACCTA CAATACCTTAACATTGGCAGCAACAACTTCATAGGACCAATCCCGAGTTCCATTTCCAAATTAATCAACCTTCAGGAACT TGGCATTTCCCATTTAAACACCCAATGTCGCCCAGTTGACTTTAGTATCTTCTCGCATCTCAAGTCGCTTGACGATCTTA GGCTATCCTATTTGACCACCACTACGATTGACTTGAACGATATCTTACCATATTTCAAGACGCTCCGTTCATTGGATCTC TCAGGCAACCTTGTTTCAGCCACAAACAAGTCTTCAGTTTCTTCAGATCCTCCTTCGCAATCGATACAATCTTTGTACTT GTCAGGATGCGGTATCACCGATTTTCCAGAGATCCTAAGAACACAACACGAATTGGGGTTTCTAGACGTTTCCAACAACA AAATCAAAGGTCAAGTTCCTGGCTGGTTATGGACGCTACCAAACTTGTTCTACTTGAATCTTTCCAACAACACTTTCATC GGTTTCCAAAGACCAACGAAACCTGAACCATCTATGGCGTACTTGCTTGGCTCCAACAATAACTTCACCGGCAAGATTCC TTCTTTCATATGTGAGTTGCGTTCTCTATACACTCTCGATTTATCAGACAACAACTTTAGCGGTTCAATCCCTCGTTGTA TGGAAAATCTCAAGAGTAATCTTTCAGAACTAAACCTTCGTCAAAATAATCTTAGTGGAGGTTTTCCAGAGCATATTTTC GAAAGTCTAAGGTCGCTTGATGTCGGCCATAACCAGCTTGTGGGAAAGCTTCCAAGATCTTTGAGATTTTTCTCTAATCT TGAAGTTTTGAACGTGGAAAGCAACAGAATCAACGACATGTTTCCGTTCTGGTTGAGTTCTCTACAAAAGCTACAAGTTC TTGTCCTTCGCTCCAATGCATTCCATGGACCAATAAATCAAGCCTTGTTCCCTAAGTTGCGAATCATCGACATATCACAT AATCACTTCAATGGAAGTTTGCCGACAGAGTATTTTGTGGAGTGGAGTAGGATGTCATCACTTGGGACATACGAAGATGG GTCGAATGTAAACTACCTGGGATCAGGCTATTACCAAGATTCAATGGTTTTGATGAATAAAGGTGTAGAGTCGGAGCTGG TACGTATCCTCACAATCTACACAGCAGTCGACTTTTCGGGAAACAAATTTGAAGGAGAGATTCCAAAGTCCATCGGTCTA TTGAAAGAGCTTCATGTGCTCAACTTGTCAAACAATGCTTTCACCGGCCACATCCCTTCATCTATAGGGAACCTGACAGC TCTCGAGTCGTTGGACGTTTCCCAGAACAAGCTTTATGGAGAAATCCCACAAGAGATAGGGAACCTCTCGTTGCTTTCGT ATATGAACTTCTCTCATAACCAGCTTACAGGCCTAGTACCAGGAGGCCAGCAGTTTCTAACGCAGCGTTGCTCTTCCTTT GAAGGTAACTTGGGACTTTTCGGCTCTTCTCTTGAAGAAGTTTGCAGAGATATTCACACGCCAGCATCACATCAACAGTT TGAAACGCCGCAAACAGAGGAAGAAGACGAAGATCTGATTAGTTGGATAGCAGCTGCAATAGGATTCGGACCTGGTATTG CCTTTGGTTTGATGTTTGGATACATACTCGTTTCCTATAAACCAGAGTGGTTCATGAACCCTTTTGGCCGAAACAATCGT AGACGGAAAAGACACACAACAACTCACTAGAGAGGTAATGTTCTGATTTTTAAAACGAATTTGTTAGCAAACATTAGAAT AAATTACGTGGACAAACTTTTGAATATAGGTCCGTATAATTGATACCGA .....N.............i............................................................ ....................................i........................................... ................................................................................ ......N..........................................................i.............. ....................i..............................................i............ ......................................N......................................... ...........................N.................................................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ....................................................................N........... .......................................i...................................N.... ................................................................................ .............i.........i........................................................ ................................................................................ ................................................................................ ........................i....................................................... ................................................................................ ................................................................................ ......N......................................................................... ............................N................................................... .................................i.............................................. .........N.....................................................................N ................................................................................ ...................N............................................................ .....................................N.......................................... ...............N........N....................................................... ..........i........................................i........................i... .....N.....................................N........N........................... ................................................................................ .............N....................i............................................. ...................................N............................................ ..N............................................................................. ................................................................................ ................................................................................ ...........N.........................................N.......................... .....................................N.......................................... ................................................. Pos Score Pred ------------------------ 6 0.372 - 20 0.742 Yes 117 0.565 Yes 247 0.455 - 306 0.671 Yes 341 0.729 Yes 388 0.526 Yes 439 0.372 - 508 0.167 - 949 0.497 - 1000 0.514 Yes 1036 0.251 - 1134 0.724 Yes 1144 0.596 Yes 1385 0.520 Yes 1607 0.252 - 1709 0.303 - 1794 0.621 Yes 1850 0.360 - 1920 0.435 - 2020 0.275 - 2118 0.178 - 2176 0.115 - 2185 0.278 - 2251 0.654 Yes 2292 0.573 Yes 2317 0.541 Yes 2326 0.498 - 2364 0.408 - 2373 0.416 - 2494 0.233 - 2515 0.547 Yes 2596 0.323 - 2643 0.234 - 2892 0.264 - 2934 0.286 - 2998 0.100 -

Explain the output -- Processing locus 47: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAATCTTTAGGGTTTTCTTAGTTTGTATTTACCTTTTTGTTTTGGAAATCAAATCTCTGGCCTCGTTTTTTTTTTTTAA TAATCCGGAGGCCTTGATTAGGGTTTTCAAAATCTAGTAGCTTTGTGTCATGATCTTTATAAGATAAGTAATAGATATAT GTATTTTTTTGCTTTTTATATCAGATACAAATTTTCAATGGCAATTTATTACAAGTTTAAGAGTGCAAGGGATTATGATA CCATTGCCATGGATGGTCCTTTTATATCCGTCGGTATTCTCAAAGACAAAATTTTCGAAACTAAGCATTTGGGCACTGGT AAAGACCTTGATATTGTCGTCTCCAATGCCCAAACTAACGAAGAGTATCTGGATGAAGCAATGTTAATCCCAAAGAATAC ATCTGTCCTTATTCGTCGGGTTCCTGGACGTCCTCGCATCACAGTTATTACCACACAAGAGCCTAGAATCCAGAATAAAG TGGAAGACGTTCAGGCTGAAACCACCAATTTTCCTGTTGCTGATCCATCTGCTGCTGAATTTCCTGAAGATGAGTATGAC GAGTTTGGGACTGATCTTTATTCCATTCCTGATACCCAAGATGCCCAGCATATTATTCCACGTCCTCATCTTGCAACCGC AGACGACAAAGTAGATGAAGAAAGCAAAATTCAGGCGCTGATTGACACTCCAGCACTTGACTGGCAACAACGACAAGGTC AAGATACTTTTGGAGCTGGTAGAGGTTATGGGAGGGGTATGCCTGGAAGGATGAATGGACGTGGTTTTGGAATGGAAAGG AAAACGCCACCTCCAGGTTATGTGTGCCATCGTTGCAATATCCCTGGACATTTTATTCAGCATTGCCCCACAAATGGAGA CCCTAACTATGATGTTAAGAGAGTTAAGCCACCTACTGGTATCCCCAAGTCCATGTTGATGGCGACCCCAGATGGTTCTT ATTCCTTGCCAAGTGGCGCAGTTGCAGTTCTGAAACCAAATGAGGATGCTTTTGAGAAGGAAATGGAGGGATTGCCATCA ACAACACGTTCTGTAGGAGAACTTCCACCTGAGCTAAAATGCCCATTATGCAAAGAAGTGATGAAAGATGCTGCGCTTAC AAGCAAATGTTGTTACAAGAGCTTCTGTGACAAGTGTATCAGAGATCACATTATTTCAAAGTCAATGTGTGTTTGTGGAA GATCAGATGTACTTGCTGACGACCTTCTACCAAACAAGACCCTCAGGGATACCATAAACCGTATTTTGGAAGCTGGAAAC GATAGCACTGAGAATGTGGGCAGCGTTGGCCATATCCCAGATTTGGAGTCTGCACGCTGTCCACCTCCCAAGGCTCTATC TCCTACTACATCTGTTGCATCTAAAGGCGAGAAGAAACCAGTACTTAGTAACAATAATGATGCTTCAACTTTAAAGGCGC CAATGGAAGTTGCAGAGATCACAAGTGCCCCTCGTGCATCTGCAGAAGTGAATGTAGAAAAGCCAGTTGATGCATGTGAA AGCACTCAAGGGTCCGTGATTGTGAAAGAAGCAACAGTGTCAAAGTTGAATACGCAAGCCCCCAAGGAAGAAATGCAGCA GCAGGTTGCTGCTGGAGAGCCAGGAAAAAAGAAGAAAAAGAAACCAAGAGTGCCTGGGAATGACATGCAATGGAATCCTG TGCCAGATCTAGCAGGCCCTGACTATATGATGCAAATGGGACCAGGTCCGCAGTACTTCAACGGCATGCAACCTGGCTTC AATGGCGTACAACCAGGCTTCAACGGTGTTCAACCTGGCTTCAATGGCTTTCACCCTGGCTTCAATGGATTTGGAGGCCC GTTTCCAGGCGCAATGCCTCCATTTATGGGTTACGGATTAAACCCAATGGACATGGGATTTGGTGGGGGTATGAATATGA TGCATCCAGATCCATTCATGGCACAAGGATTTGGGTTCCCTAATATACCACCACCCCATAGGGACCTAGCTGAAATGGGA AACCGAATGAATCTCCAACGTGCTATGATGGGCAGAGATGAAGCCGAAGCCCGAAACGCTGAGATGTTAAGAAAACGTGA AAACGAGAGACGACCTGAAGGTGGGAAGATGTTTAGGGATGGAGAGAATAGCAGAATGATGATGAACAACGGTACTTCAG CCTCTGCATCATCCATCAACCCTAATAAATCTAGACAAGCACCTCCACCACCAATCCACGATTACGATCGCCGGAGACGA CCGGAGAAGAGACTATCCCCAGAGCATCCGCCTACGCGTAAAAACATTTCCCCGTCTCGCGACTCCAAGAGAAAATCCGA GCGTTATCCTGATGAACGAGATCGGCAACGCGACCGTGAGAGGTCACGTCACCAAGACGTAGATCGTGAACACGACCGTA CTCGCGACCGTCGCGATGAAGACAGAAGCCGAGATCATCGCCACCACCGTGGTGAAACCGAACGCAGCCAACATCACCAC CGCAAGAGATCGGAACCGCCTTCATCGGAGCCACCAGTCCCTGCTACTAAAGCAGAGATCGAGAATAATCTTAAGTCTAG CGTATTCGCTCGGATAAGTTTTCCGGAGGAAGAAACATCTTCTGGTAAACGAAGAAAGGTGCCTTCCTCTTCTTCTACTT CTGTTACGGATCCATCTGCTTCCGCCTCTGCCGCAGCCGCCGTGGGAACATCAGTTCACCGACACAGTAGTCGGAAGGAA ATAGAAGTAGCAGATTATGAATCTAGTGATGAAGATAGGCATTTCAAGAGGAAGCCGTCAAGGTACGCGCGATCACCACC GGTGGTGGTTAGTGATGTGAGTGAAGATAAACTCCGATACTCAAAACGTGGAAAGGGGGAGAGATCTCGAGCTTGACATG GATTTTCAAACATGTTTTTTTTTTTTTTTGTATAATCAAAAACAGAGAATGAGAATCTTCTTGTTTTGCTGTAACGAACA AAGAGAGAGAGTTCT ................................................................................ .................................................N............................i. .....................................i....................................N..... ........i...N................................................................... .........................N..........................N.......i................... ................................................................................ .....................................................................N.....N.... ........................................N....................................... ..............i................................................................. ...........................N..........i...........N...N................i........ ...................N.....................................................N...... ........N..N........................................N.....i............N........ .......................................N.....N................i................. ......................................N........N............N......i............ ......N.........................................................i............... ......N......................................................................... .............i.................................................................. ........................................................N..N.................... ..i................................................N.................N...i...... ........................................................................i....... ...........................................................N....N....i.......... ..........................N..N.....N.............................N.............. .............i...........N....................i.....i.................N.....i..N .................i........................................................i..... ......i.................i..i.........i.........................i................ ............................i.........N................i..i..i.................. ................................................................................ ................................................................................ ...........i.................................................................... ...............i................................................................ ................................................................................ ................................................................................ ................................................................................ ................N...........N................................................... ..............N..............................................................N.. ...........N....................................N............................... ............... Pos Score Pred ------------------------ 130 0.109 - 159 0.539 Yes 198 0.726 Yes 235 0.284 - 249 0.833 Yes 253 0.135 - 346 0.425 - 373 0.097 - 381 0.728 Yes 550 0.293 - 556 0.207 - 601 0.281 - 655 0.704 Yes 748 0.314 - 759 0.598 Yes 771 0.348 - 775 0.334 - 792 0.620 Yes 820 0.253 - 874 0.302 - 889 0.312 - 892 0.169 - 933 0.303 - 939 0.765 Yes 952 0.360 - 1000 0.372 - 1006 0.234 - 1023 0.643 Yes 1079 0.319 - 1088 0.191 - 1101 0.318 - 1108 0.598 Yes 1127 0.158 - 1185 0.611 Yes 1207 0.344 - 1294 0.546 Yes 1417 0.301 - 1420 0.395 - 1443 0.894 Yes 1492 0.268 - 1510 0.293 - 1514 0.580 Yes 1593 0.555 Yes 1660 0.406 - 1665 0.298 - 1670 0.630 Yes 1707 0.391 - 1710 0.483 - 1716 0.383 - 1746 0.256 - 1762 0.603 Yes 1804 0.295 - 1825 0.261 - 1854 0.576 Yes 1866 0.468 - 1887 0.563 Yes 1893 0.680 Yes 1911 0.350 - 1917 0.515 Yes 1920 0.419 - 1938 0.638 Yes 1995 0.793 Yes 2007 0.761 Yes 2025 0.694 Yes 2028 0.863 Yes 2038 0.701 Yes 2064 0.626 Yes 2109 0.738 Yes 2119 0.445 - 2136 0.735 Yes 2139 0.586 Yes 2142 0.526 Yes 2332 0.716 Yes 2416 0.731 Yes 2737 0.293 - 2749 0.298 - 2815 0.303 - 2878 0.211 - 2892 0.336 - 2929 0.070 -

Explain the output -- Processing locus 48: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGCTGTGACACTCTTTTGACTTCATCTTCGTCTTAGCACATTGCATCACATGCCTTGATGTCTAAACTATAAAATGACT TTGAATCATCATTACATTTGATATTTGCACTTAATGTCTTCCTCGAAGTTAATGGACTTTGGCCTAACATGGATTATAAT GATGATGATATTGTTACAGGGATGTAGAAGCTGCATTGAAAGTGAAAGGCAGGGTTTGTTGGAGATCAAGGCTTATATTA TCTCGGTTATTACTGATCCACATTTAGACATTCGTCGAGGATGGATGAGTAGTGATCGGTCTTGTTGCCATTGGAGAAGA ATCAAGTGCGACATCACCAGCAAACGCGTGATCGGAATCTCCCTATCTTTAGAATCCATACGTCCCCCAGATCCTCTTCC CCAATTGAACCTAACTTTTTTCTATCCCTTTGAAGAGCTTCAGAGTCTCAACTTGTCGTCGGGGTACTTCAAAGGCTGGT TCGACGAAAGAAAAGGTGGTAAGGGCCTGGGAAGTTTAAGAAATCTTGAGACTCTGGATCTCGGTGTCAACTTTTACGAT ACCAGTGTCTTGCCATACTTGAATGAGGCTGTGTCGCTCAAGACTTTGATTCTCCATGACAACTTATTTAAGGGTGGCTT TCCTGTTCAAGAACTAATAAATCTGACAAGTTTGGAAGTACTAGATCTGAAATTCAACAAGTTCAGTGGCCAGTTACCAA CACAAGAGCTCACTAATCTGCGAAACCTCAGAGCCTTGGATCTAAGCAATAATAAATTCTCTGGTTCATTGCAAAAACAA GGAATTTGTAGATTAGAGCAGTTGCAAGAGCTTCGTCTTAGTCGAAACAGATTTGAGGGTGAAATCCCACTCTGTTTTTC GAGATTCTCCAAACTCCGAGTTCTTGATCTATCATCAAATCACCTAAGTGGGAAGATTCCATATTTCATCAGTGACTTCA AATCCATGGAATACTTATCACTTCTCGATAATGACTTTGAAGGCTTGTTCTCGTTGGGTTTGATCACTGAATTAACAGAG CTCAAGGTTTTTAAACTCTCTTCCAGGTCTGGTATGCTGCAAATAGTAGAGACTAACGTTTCCGGTGGTCTGCAATCGCA GCTAAGTAGTATTATGTTGTCACACTGCAACCTTGGAAAAATCCCTGGTTTTCTTTGGTATCAGCAGGAATTGCGGGTCA TCGATCTCTCCAACAATATATTATCCGGAGTGTTCCCCACTTGGCTGCTGGAAAATAACACAGAGCTTCAGGCCCTGTTG TTACAGAACAACTCATTCAAAACACTTACACTTCCAAGAACCATGCGCAGGCTGCAGATTTTGGATCTTTCGGTGAATAA TTTCAACAATCAACTCCCTAAGGATGTCGGTTTGATACTTGCGAGCTTGAGACATTTGAATCTCTCAAACAATGAGTTTC TAGGGAACATGCCCTCCTCTATGGCCAGGATGGAAAATATTGAATTTATGGACCTATCTTACAACAACTTCTCAGGGAAA TTGCCAAGGAATCTTTTCACAGGTTGCTACTCATTGAGTTGGCTTAAGCTCTCGCACAACAGATTCAGTGGCCCGATTAT TCGGAAATCGAGTGATGAGACCTCTTTGATTACGCTGATCATGGACAATAACATGTTTACAGGGAAGATCCCTCGTACTC TACTCAACTTAAGGATGTTGAGTGTGATCGACTTATCAAACAACTTACTCACTGGTACAATTCCAAGGTGGTTAGGCAAT TTCTTTTTGGAAGTTCTGAGGATCTCCAACAACCGTCTACAAGGTGCCATACCACCGTCTTTGTTCAACATTCCTTACCT CTGGCTCTTGGACCTTTCTGGAAACTTCTTGTCGGGTAGCTTACCCCTGCGGTCCAGCTCAGATTACGGTTATATATTGG ACTTGCACAACAACAATCTCACTGGATCTATTCCAGACACGTTGTGGTACGGGCTACGGTTGCTTGATCTGAGAAACAAC AAACTGTCTGGAAACATTCCACTGTTCAGGAGCACCCCGAGCATCAGTGTTGTTCTGTTGAGAGAGAATAACTTGACAGG AAAGATTCCTGTTGAGCTGTGCGGTTTAAGCAACGTAAGGATGTTGGATTTTGCTCACAATCGATTGAATGAATCCATAC CTTCGTGTGTCACCAATCTATCATTTGGCTCAGGAGGACATAGTAATGCTGATTCAGATTGGTATCCGGCGAGCTTGCTC TCAAACTTTATGGAGATATACACCGAAGTATATTACGAGTCTTTGATTGTGTCAGATCGGTTTTCTCTAGACTACTCGGT AGATTTTAATGTCCAAGTTGAATTTGCGGTGAAACAGAGATATGACTTGTACATGAGAGGAACTCTTAACCAGATGTTTG GCCTGGATCTGTCGAGCAACGAGTTAAGTGGTAATATCCCAGAAGAGCTAGGAGATCTTAAGAGAGTACGCTCGCTGAAT CTCTCACGCAATTCATTATCGGGTTCTATACCTGGAAGCTTCTCCAACCTAAGAAGCATAGAGAGCTTGGATCTTTCTTT CAACAAGTTGCATGGAACCATTCCCTCACAACTCACATTGTTGCAATCTCTTGTCGTCTTTAACGTGTCATACAACAATC TCTCAGGTGTGATTCCGCAAGGAAAACAGTTCAACACCTTTGGTGAGAAGAGTTACTTAGGTAATTTTCTTCTCTGTGGA TCACCCACCAAAAGAAGCTGTGGTGGTACCACAATCAGCTCAGGAAAAGAATATGAAGATGACGATGAAAGTGGATTACT CGATATTGTGGTATTGTGGTGGAGCCTCGGGACGACCTACGTAACGGTTATGATGGGGTTTTTGGTGTTTCTCTGCTTTG ATTCTCCATGGCGCCGAGCATGGTTCTGCCTTGTTGATACTTTTATCGACCGCGTAAAAGATGTACTGGGAGTTATCTAG AGAGGGTTCGTGGACGTGGAGGATGAAACAATAAAATGTAATAGTTGAATCTTGCAAATATTTTGATCATATTGTCAAAT TTCTCCATCGTATAAAACT ..................................................N.......N...............N..... .................................N.................i................i.........i. ........................................i...i................................... ................................................................................ ................................................................................ ................................................................................ ......................N................................N........................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ .....N........................N................................................. .................................N.............................................. .............N.................................................................. ................................................................................ ..........................................i..................................... .......................N...............................................i........ ........N...........N........N.................N................................ ................................................................................ ..............N.........................N...........N........................... ..............N................................................................. ................................................................................ ................................................................................ ................................................................................ ................................................................................ ........................................i...........................N........... .............................................N.................................. .........N...................................................................... ........N................................N..........N....................N...... ................................................................................ ................................................................................ ...........N.................................................................... ................................................................................ ....................................................N.....N.....N............... .................................................N..N........................... .......i...........N........................................N................... ......................N............N............................................ ................... Pos Score Pred ------------------------ 51 0.225 - 59 0.375 - 75 0.325 - 114 0.360 - 132 0.528 Yes 149 0.576 Yes 159 0.512 Yes 162 0.130 - 165 0.227 - 182 0.318 - 281 0.712 Yes 285 0.584 Yes 583 0.160 - 616 0.226 - 966 0.320 - 991 0.389 - 1074 0.232 - 1134 0.346 - 1323 0.672 Yes 1384 0.392 - 1432 0.537 Yes 1449 0.187 - 1461 0.412 - 1470 0.366 - 1488 0.128 - 1615 0.098 - 1641 0.353 - 1653 0.284 - 1695 0.323 - 2121 0.610 Yes 2149 0.120 - 2206 0.202 - 2250 0.415 - 2329 0.113 - 2362 0.400 - 2373 0.455 - 2394 0.264 - 2572 0.346 - 2773 0.213 - 2779 0.235 - 2785 0.191 - 2850 0.228 - 2853 0.410 - 2888 0.571 Yes 2900 0.484 - 2941 0.215 - 2983 0.137 - 2996 0.159 -

Explain the output -- Processing locus 49: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCGACATGAATCTTGCCCCAATCTTCAGGTACTCTCTTCTATTTTGTTTCCCTTCTCCTCTTGTGCGTTTTGCTGCAATT TCCGATCCTCATAAACCCTAGCTTTCTGACGCATATTCAGCAACTAAAACGATGAAGAGACTAAGATCCAGCGACGACCT TGATTTCTGTAATGATAAGAATGTTGACGGAGAACCTCCCAACTCGGACCGTCCGGCCTCTTCCTCCCACCGGGGCTTCT TCTCGGGCAACAACCGTGACCGCGGGGAAGATGCTGCCGGCTTCTCCAGGGCTTTTTCCCGTAGGCGATCCAATCGTGAC TTGGATAATCATCGGCCAGATGCCCGGTACCATCGATCTGAGAGCGCCTGTTTCTCAAGGAGGGCTTTTCCAAAGGGATT TCGTTCTGAGAGGGAGAGGCCCAATAGGGATGCTAGTGTCTCTTCCTGGAGGAGGTTTGGTGGTCCTGGTAATGACTTTG GCGTTGATGACCGAGATAGGAGATTAAGGGACGCTGAGAGGGATAGGAGTCTCAAATCCCCTTCTTGGTCCAGAGACTCG CCTAACGAGCTTTCCAAATTCAAACCACTCGATTCCAGAAATTCTCGCTCCAGATCCAAATCTCTAGCTTCTCCGACATG GTCCAAAGATTCCGGGAGTGAGCAATCCAAAAGTGTTGGAAATGTTGTCAAGAAAAGCGAGGAGGAAGTGCAGGGTAAGA GCAGTACAACCAGTAGCGAGATGGAGGAAGGAGAGCTGGAGCCAGAGCCCCAACCAGAGACTGCCTCAGGCCTTGCTCAT CAAACCAAACATGACTGTAAACTGCCTTCCTGCTCTGCTGATGATCATAAGAACGCAAGGATTGATAGAAGCTTCCAGGA AATTGGAAAAAGCGCTCAACTTGATGCCAACACTGAGAGTAACAGGGAACTCAGTCATGTGGGTGGTAATAGGGAAATGG AAACGACAGATAGCATGACCGATAAGAAAAGTGTTGAAGACGCGGAAAATGTGCCTGAGCATGCGACTGAGTCCATGCAT GTTTCTCAAAACAACGTGAATGATACTTCTACAGCTTTGGCTATTGAACACGATCATAGAGATGGCACAATCACTGCTAG TGCCAATAAGATTACAGATACGGTTGACGAGAAAGGTGACAAAGATGAGGATTATAAGGAGAACCTTCATGGAGTGAAGT TGGAAGAAACTCTTTATCCAGATGTTCCTGAACGGTTGGAAGAGCTTAAGGAAGTGAAGGGTAATGATGGTGATGCCAAC AAAGCGGAAGTTGAAGGCCCGGAATGTGTTGAGGAAAATGCTCTAGCTAATAGAACGCCTGCAGAATACATTAGCAGTGT ATCGGATAGCTCCGTCCACAAATGCAAGGATAAAGGCAAAAATTCTGATGTCCCTCTTACACATTTAGTTGGAAATGCTC TCTTTTCTGAAAGCAAGACTGAGGATCTTCATGACAAGGATAAAGATGAAAAAGATGACAACTTCGGAGGACCGAGTATC AGAGGGTTTGAACTGTTCTCTAGTTCTCCGGTTAGGAGAGCAAAAAAAACTGAGCAGTCAGGTGTCAATAAGCACAAGGA TGAGAAGTTGTTGTTGGAGCCACTGGATCTGTCACTTAGCCTACCAGATGTATTGTTGCCGATTGGTGGCCAGGATACTA ATCAGCTGGGGTCACCAGTCCGTTCTGGAAGTGTTCGGTCTTTGACAGACACGTTTTGTACCAATTCTGATGGCTTTACA ATGTCTATGTCCTTTTCAGGTTCCCGTTCGTTCAATCATAATCCAAGCTGTTCGCTGAGTCACAATATTGGAGACAATGA GCAGTCAGTTCATAGCCGACCTATATTTCAGGGTATAGATTGGCAGGCACTGTCTCATAATGATTCAAAGTACAATGAGA ATACAGTCTATCAAAGACTGATGGAAAATGGAAATGGTTCAGTTCAACCTCGAGCAATGAAAGGCAATTTAATTTCGGGT CAAGCTGATGAAGAGCATCTTAGATTGCCCGATGGGAGCTCTAAGGCAGCAAATATACTTGAGAAACAATTGAGTTTCCA GAAAAGTGTTGATGTTAGATCAGCATGTCCAAGAACGGGCTCTCTTGAAAATGGTTCAAAGTTTACTGTTGAGAAGAAAA CGGCCAAGGACTTTTACAGTGGTAGTAATTCGTGGATAACTGGGCTGGAAGCCGGTGGGCATGATTTTGTTGAGACCGTC ATCAGATACATTCTCTCCGATTCGATGCCTGTAATGACCAAGAGATTTCATGAAATGCCCACTCGAAACATCACTAGCCT GAAGGAAAACATTCGGCAGATGATGCTGAACATGGATAAGAATGTACAACTTGGTGCTTTTCAAGATGCTTTGCAGAACA GGACTGATATAACACTGGAATTGCTTACAAAATCTCATCGCGCTCAGTTGGAGATCTTGGTCGCACTGAAATCTGGGCGT TCTGATTTCCTTCTTTTGGACAACAGCATCTCGTCTTCCCATTTAGCTGAGATTTTCATGAACATGAGATGCAAAAATCT TTCTTGTAGGGTTCTGTTGCCTGTGGACGAATGCGATTGCAGGGTTTGTTCAAGGAAGGATGGATTTTGTAGTGCTTGCA TGTGTCTTGTGTGCTCAAATTTTGATATGGCATCCAACACCTGTAGCTGGGTTGGGTGTGATGTGTGCCTACACTGGTGC CACACAGATTGTGGGATAAAAGAGTCTTACATCCGGAATGGGATCAATGCTTCTGGTGCTCCAGGAATGACCGAGATGCA ATTTCACTGTGTTGCCTGTAACCATCCCTCAGAGATGTTTGGCTTTGTCAAAGAAGTGTTTCTGAATTTTGCAAGGGAAT GGAAATTTGAGCGCTTTTGCAAGGAACTGGAGTATGTCAATAAAATATTTTCATCAAGCAAAGACTCTAGAGGGAAACAG CTGAGACAAGCTGCTGATGCGATGCTGGCAAGTTTGAAAAGTAAATTGATAGGCCTTCCTGAAGCTTGCAATCGCATATT GGGTTTCATTTCAGACTGTGATTCCTCCACTCCTGCTGAAACTAGTGCACCGTTCATATACGAACAGCCAAAACCCAGGC ATGAGAGGGGAAGCCCTAGTCAGGACACGGCGTGGCTGAGATCGGTGTGCTCAGATAACCCGCATAATCAGCTGAAAAGG TCAGCGAGCGTAGCTGATGCATTCCACAGAGAGAGGCAAGTTGAAATATGTGCAGTGGAGATGGAGTTGGAAAGGGGTTC ACCAAAGGAACCTCGTTTCGAAGAGCTAGAGAGCATTGTGAGAATGAAGCAAGCAGAGGCAGAAATGTTCCAAGGGCGGG CCGATGATGCACGAAGAGAAGCAGAGGGACTGAAACGAATAGCAATAGCGAAGAAGGAGAAGATAGAAGAAGAGTACAAT CGGAGAATGGGGAAGTTGAGTATGGAGGATGCACAGGAGAGAAGGAGGAGGAGGTACGAGGAGTTAGAGGCAATGCAGAG AGGGCAGAGAGAGTTTTATGAAATGAAGATGAGAATGGAGGAAGAGATGAGAGGGCTTCTCACCAAAATGGAAATGACGA AGCAGAGTCTGGCCTTGTAATATTTGTAGTAGTAAATCACATGTAATGTACACAGAAACCTCCTCCTCGTATGTAACCTA AAGATCAATGTAAGGTCTTTTCTTTTAGAGAGTACTAAA .....N.......................................................................... ...................................................i............................ ...........N........i........................................................... ..............................N................................................. ...................i............................................................ .............................i.........................................N........ ......N......................................................................... .............................................................................N.. .........................................i...................................... ....................i........................................................... ..........N.............................N....................................... .......................N................................N...................i... ..............i.................................i...........N.............i...N. ...................N.........................................N.................. ............................................N.......................N........... .....................N.........................................N..N.....N....... .......................N.............N.......................................... .....................N.........................N..........................N..... ..............................N..............N........N......................... ...............................................................................N ...............................................N................................ .....................................................................N.......... i.....N.....................................................................N... ...........................................................N..............N..... ....................i......i.....N......................i....................... .......N.......................N................................................ ...........N............N.........................N............................. ............................................................N................... ........................N........N...............N....i......................... ...................N..N........i.........N.......................N.............. ................................................................................ .........................................................N.....N....N........... ..............................N............................i...................i ..........................i.................................i................... .....................................N........N...................i........N.... ..................................N...........................................i. .................................i.............................................. ................N....N.......................................................... ................................................................................ i............................................................................... ................N..............................i............i................... ...........................................i....................i............... ...N..N......................................................................... ......i..............N......N...........................................N....... .................N....N.....N.....N...........N....................N.....N...... ........................................N....N........................N......... .......N............................... Pos Score Pred ------------------------ 6 0.444 - 132 0.867 Yes 172 0.464 - 181 0.739 Yes 271 0.314 - 340 0.512 Yes 430 0.540 Yes 472 0.406 - 487 0.181 - 638 0.463 - 682 0.504 Yes 741 0.540 Yes 811 0.437 - 841 0.146 - 904 0.395 - 937 0.197 - 957 0.845 Yes 975 0.831 Yes 1009 0.520 Yes 1021 0.091 - 1035 0.628 Yes 1039 0.045 - 1060 0.244 - 1102 0.202 - 1165 0.263 - 1189 0.224 - 1222 0.104 - 1264 0.281 - 1267 0.233 - 1273 0.111 - 1304 0.258 - 1318 0.145 - 1382 0.193 - 1408 0.237 - 1435 0.207 - 1471 0.213 - 1486 0.482 - 1495 0.500 - 1600 0.187 - 1648 0.483 - 1750 0.324 - 1761 0.671 Yes 1767 0.259 - 1837 0.419 - 1900 0.232 - 1915 0.322 - 1941 0.716 Yes 1948 0.532 Yes 1954 0.278 - 1977 0.565 Yes 2008 0.180 - 2032 0.167 - 2092 0.194 - 2105 0.461 - 2131 0.181 - 2221 0.152 - 2265 0.260 - 2274 0.364 - 2290 0.105 - 2295 0.761 Yes 2340 0.363 - 2343 0.360 - 2352 0.619 Yes 2362 0.142 - 2386 0.305 - 2538 0.419 - 2544 0.233 - 2549 0.227 - 2591 0.136 - 2620 0.507 Yes 2640 0.544 Yes 2667 0.657 Yes 2701 0.549 Yes 2758 0.179 - 2767 0.154 - 2787 0.576 Yes 2796 0.256 - 2835 0.224 - 2879 0.537 Yes 2914 0.616 Yes 2977 0.167 - 2982 0.453 - 3121 0.642 Yes 3217 0.291 - 3248 0.695 Yes 3261 0.728 Yes 3324 0.583 Yes 3345 0.619 Yes 3364 0.224 - 3367 0.298 - 3447 0.587 Yes 3462 0.366 - 3469 0.249 - 3513 0.253 - 3538 0.083 - 3543 0.176 - 3549 0.151 - 3555 0.410 - 3567 0.200 - 3588 0.137 - 3594 0.209 - 3641 0.119 - 3646 0.108 - 3671 0.044 - 3688 0.054 -

Explain the output -- Processing locus 50: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAATGACTTGCATATATATAGGATTTGGTCTTTTGTTACAATGACTAGCATTTAGTTATGTCATTTGTTTGTGTCTCAT TGTTGATTCTGTTTTGTATGATCAATATCTGGAAATGTGTGTGGTAAAGGTTTTGATTTGAGTACCCTAGAGATAATATT GTTTATTATGAGATCATTCTTTTGTAAGATGAATTTACAAGCTTTATGTTTTTTAAATTTTAATACTTTAGGGTTCATGG AAGATCAACCCATTAACCAGTGCTCTAGTAGCACCAATGCGTCGGAGAAAACTCCAGAATCAACCCTTGAGCTTAACATC AAAACGTTAGACTCGCGGACGTATACTTTCCAAGTGAACAAGAATGAAACTGTTTTGCTATTCAAGGAGAAGATTGCTAG TGAGACAGGGGTGCCGGTTGGTCAGCAGCGGCTCATTTTCCGAGGACGGGTTTTGAAAGATGATCATCCTCTCTCGGAGT ATCATTTGGAAAATGGGCATACTTTACACTTGATTGTGAGGCAGCCAGCAGAATCAGCTCCTTCCTCTGGTACACCTTCA CAAGGGGCAACTGCAAATGATGGAAATAATACAAATGGTGGACCATCTCGAAATGGAAGACATGTCTCCCACAGCGTAGT TCTTGGGTCTTTTAATGTTGGAGATCAGACAGAAGGCATTGTTCCAGACCTCAGCCGGGTCATTGGAGCAGTTCTAAATT CTTTTGGAGTTAGTGGACAGCTTCCTACAAATCACAGTACTAATGGCACGCAATCCTCTATGCCCTCGAATCAGTCGTCT AATGCACCTCCTGGAAATACATCTGATGGAGAGCCTGGCATTGGTGGTCAAAGCCAAGCTACGGGTCATTCACAACCTAG GCAAGCATTTCCTGGTGTATCATTTCAAACATCGATGCCTCGTGTAGTTCAGATTCCTGTTACAGCAGCCACAACGATTC CTATCCCTTCATTCTTAACGCCTATTCCGGATTCCTTAGACACTCTAATGGAGTTCATTAATCGGATGGAGCAGGCATTA TCACAAAATGGCTATCAGCCAGATACCTCTTCTGCTGGATCCGGAGGGCGACCAAGGGAAGAGTTGCCTAGAAATAGACG AGGTGCAGCAACACCTGAAGCACTCTCTGTTGTCCTAAGGAATGCACAACATTTACTGAGTGGTCTCGGTGTCTCTTCTT TATCGCATATTGCGGGACGTCTGGAGCAAGATGGTTCATCTTCAGATCCTACTCTAAGGTCACAAATCCAGACAGAGGCC GTGCAAGTAGGTCTTGCCATGCAACATTTAGGGGCCCTCCTACTGGAGCTTGGACGCACAATTTTGACATTGAGAATGGC ACCGTCGCCGGAATTATCCTATGTGAATGCAGGGCCTGCTGTGTATATATCTCCATCAGGACCGAATCCTATTATGGTTC AGCCTTTTCCTCACCAAATCAGCCCTCTCTTTACTGGTGCTACTGTCTCATCAAATCCACTAACCGGACCTGTTGGTTTA GGAACTGCCCAAAGGCACATTAATATTCACATACATGCTGGCACATCAGGTTCACCTATGTTGTCATCAGTTGGGAACCA GCGAAGCAATGGAGAAGGAGGACAAGGAGACCGTGACAGCAATACAAGTTCAGTTCCTGCGGCTGTTCCATCACACTCTA CCGGTGAGAACGTTTCTGCTGGGGTTCAACCTGGTTTGGGTGATGATGTTTCAGTTGCTCAAATCAATGCAAGGATAAGA GATATGGTAAACATTATGCAAGGAAGAGACCAGATTCCATCGGGAATAGAATCTCTCGAGAGGGATATGTCTACGGGCCA TGGTGTTGCCACTGCTATGCCTGAGCAGCCTACCAACATTGCAACTACTTGCGCACCTGAGTCGTCTAGTGGCTCACTGC ATGACTTACCATCTGAGAGGAGCAACTCAGTGTGTCAAAATGAAAAAGATCTTGGTGGAGATTTAGAGCATCCAGCCCGA GCAAAAGATACCTCTTGTACTACAGGACAATCATCAGCTCCGTCTGGAGATGCTACCGGTGATGCCAAAGAAACCAATAA GGCTACTCCAGAGGTAGCTACAGCTACACCACTTGGATTGGGGCTTGGCGGTTTAGACCGTAAGAAACGAAGCAAGCAGC CGAAGGTATCAGGTAAGACTGAAGATAGCGGGACTTCAGCTACACTTGAAGGTGTGCAACAAAGCAGTGGGACCAGTGGT CAGCAGCTTTTGCAATCTCTCTTTTCTGGTAGTTCTCGTTCAGATGAGACTGGTCTTAGACGCGGGCAAGGTTCAGATGA TCGTGTAGATGTCTCAAGTGCAATGTCTCAGGTCCTGGAGAGCCCTGTATTGGATGGATTGCTAGCAGGGGTATCTCGAC AAGCTGGTGTTGATTCACCAAATATGCTGAGAAATATGCTCCAGCAGTTTACCCAGAATCCACAGATCATGAACACAGTC CAACAGATTGCGCAACAGGTGGATGGGCAAGAGATAGAAAATATGATGTCAGGTGGAGCTCAAGGCGAGGGCGGCGGCTT TGATTTCTCTAGAATGGTCCAACAAATGATGCCTCTAGTCTCACGTGCTTTTAGCCAAGGAGGGCCATTGCCTCATCCCG CAACAATACAGCCTGATGATCGTCAACCTTCTCAGGTAAATGTTCAATCCATGGCTCAAATGATTGAGCACTCTGATCCA CCAGAGGATGTCTTCCGTGCAATGGTCGAAAATGCTGCGATAAGCCAAGACGAACTAGTGAACGAGCTTTGCTGTGATGA AGCTCTTTCTCAAGAATATGCGGAGTTGCTAAGACGTGATATCGAAGGCCGGCTAAAAGATGATCAAGGTCTATAAGATA TTTGGTTGAATTGGTTCTGTAAGTGTCATATCTTTGAAGTATCTTTGTTTATTTGCTGAGATCCCTCACTTATGTATTAA GAATTAGGGTTTGGG ...i.....................................N................N..................... .................N................i............................................. .......N....................N................i..............................i... ....................................N........................................... ...........................................N.................................... ...........................................................N.................... ............N................................................................... ................i..i..............i.................i........i.................. ..............N................................................................. ..........................................i................i.................... ..................................i............................................. ...............................................i.................N.............. .......i........................................................................ .........................................N...................................... ..............................N................................................. ..................N........................................................i.... ....................N.....N..............................................i...... ................................................................................ ..................................i......................i...................... ........N....................................................................... ..........................................N..N....................N............. ...i...........i..................................................i............N ................i............................................................... i......................................i........................................ .................................................N...........N.................. ................................................................................ ................................................................................ ...........................................N................................N... ........N.............i..............................N.......................... .......................N...........i................................i........... ......................N...................i..i.................................. .............i...........i..i................................................... ...............N.......................N..........i........i.................... .......N.............i.........i............................................N... .................N.........................................i.................... .......................................................................N........ ............... Pos Score Pred ------------------------ 4 0.500 - 42 0.226 - 59 0.232 - 98 0.137 - 115 0.588 Yes 168 0.194 - 189 0.360 - 206 0.501 Yes 237 0.522 Yes 277 0.169 - 364 0.202 - 460 0.289 - 493 0.304 - 577 0.574 Yes 580 0.521 Yes 595 0.544 Yes 613 0.595 Yes 622 0.540 Yes 655 0.420 - 763 0.531 Yes 780 0.754 Yes 802 0.173 - 826 0.653 Yes 915 0.617 Yes 1008 0.780 Yes 1026 0.490 - 1048 0.805 Yes 1162 0.475 - 1231 0.248 - 1299 0.350 - 1356 0.625 Yes 1381 0.116 - 1387 0.229 - 1434 0.527 Yes 1555 0.634 Yes 1578 0.650 Yes 1609 0.351 - 1723 0.170 - 1726 0.190 - 1747 0.254 - 1764 0.769 Yes 1776 0.717 Yes 1827 0.589 Yes 1840 0.326 - 1857 0.561 Yes 1921 0.541 Yes 1960 0.507 Yes 2050 0.371 - 2062 0.316 - 2284 0.258 - 2317 0.264 - 2329 0.254 - 2343 0.732 Yes 2374 0.253 - 2424 0.453 - 2436 0.556 Yes 2469 0.652 Yes 2503 0.415 - 2523 0.548 Yes 2526 0.611 Yes 2574 0.638 Yes 2586 0.630 Yes 2589 0.790 Yes 2656 0.312 - 2680 0.124 - 2691 0.811 Yes 2700 0.714 Yes 2728 0.355 - 2742 0.741 Yes 2752 0.595 Yes 2797 0.114 - 2818 0.196 - 2860 0.575 Yes 2952 0.064 -

Explain the output -- Processing locus 51: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAATCTGAATAAAATTAGATTTAGGGAAACGTAAAGACTTCATTATGGAATAAATCGTGAGGACTTTTAAGTCATTTTG CATCTCACTCTCACGCCGTAACAGTCTCTCTCTATGACACAAAGCGACGGTGTTCCCGTTCTGTTCCGTTCCGTTAAGTT TCGGACTTATATTACAGAACTGAACAATCGTTGTCTTCAAGTACGAGATTTGTCTGATTTTTCCAAAGCGCCGATTCGAA GATTTGAATCCATTTCAGTGATGCCGTTTTGGAAGATGAGCGCAAGAGATGTCACTGGATTCAAATTCCTCTTCTCACTC GTCTTCATCTACGCTTTAATGTCAGCTATTGTTTACTCCGTCCTCCACATGAAATTCATCTCTCCGCTTCCGGCAAACGC TCCTCTCGAACGATTCTCTGAAGCTAGAGCCGTCGAACACATCCGTGTCTTGGCTGAAGAGATCGATGGTCGTCAGGAAG GACGACCTGGACTTAAAGAAGCTGCAACGTATATAAAATCGCAATTGGAGATGGTGAAGGAGAGAGCTGGACCAAATTTA AGGGTTGAAGTGGAAGAGACTCAAGTGGATGGTTCCTTTAGCATGATGTTTCTAGGACACAGCATATCACTTGGATACAG AAACCATACTAATATACTTATGCGTATCTCGTCAATGAATTCTCTTGACACTGATGCATCTGTATTGATGAATGCACATT ATGACAGTCCTGTTAACTCTCCTGGAGCTGGTGATTGTGGGTCATGTGTAGCATCACTACTTGAATTAGCAAGACTGGTA GTGGATTCTGGTTGGGTCCCGCCTCAACCTGTAATTTTTCTCTTCAATGGTGCTGAAGAGCTTTTTATGCTGGGCTCACA CGGCTTCATGACGAAGCATAAGTTGAAAGATACCATTGGAGCTTTTATAAACGTGGAAGCCTCTGGGACAGGGGGTATTG ATCTGGTCTGCCAATCAGGGCCTGGCTCGTGGCCATCTTATGTCTACTCTCAAGCAGCAGTGTATCCAATGGCACAGAGT TCTGCACAGGATGTTTTCCCTGTAATTCCTGGAGACACAGATTACAGAATGTTTGCAGAAGATTATGCCGACATTCCCGG GCTAGACATTATCTTTCTTCTGGGCGGTTACTACTACCATACTACCTTCGATACAGTGGACAGAATAGTACCTGGAAGCA TGCAAGCACGTGGAGAAAACTTAATCAGCGTGCTTAAAGCCTTCGCGAGTTCTTCTAGGCTAAAGGTTGCTAGTGAAAGA AAGACGCTTGACGTGGATGCCAACAGTGACATGGTTGAAAGAGCTGTTTTCTTTGATTACTTAACATGGTTCATGGTGTT CTACCCAAGAAGAGTAGCTTTCGTACTTCACAACATTCCAGCTGCTTTGTTCCTTTGTGTTCCTTTCTTTTTGTATATGA TGGACCCTAGGACACACCCTTTGTTATCATTCTTCTGGGCTTTCTTTAAAGGAGTTATGCACCATTTCGCCGGGATCTTA CTTGGTGTAATAGTCCCTGTTCTCTTTGCAGTCATCAGATTGTTCTTTGCTTATCCCATGAGCTGGTTTGCTCATTCATA CTTAGCTTTCTTGATGTTCATCCCCTGCTCATTTTTTGGCCTTTTAATTCCAAGAGCTATCTCTGATCGTGTCTCACATT TTCAAGGTGTTTCATCCAAGAAAATTATGAAAGAACCATCTGATGAAGCAAGGTTTTGGGGAGCATTTGGGTTCTATGCT TTTGCAACTTCGGCATATTTTTTCGCTGGATTAAATGGAGGATTCATGACATTTGTCATCTCCATTTCTATGCTACTAGG GTGGATTGCTTTCTATTTGTCAGTCAAGTCCTATGGCTATAATTCGATCAAGTCTCCCATGTTTTATGTGATAGCTCTTG TTCCATGCCTTCTGTATTCTCTCTATTTTGGTGGTATTCTTACACTATTATTAATTGAGAAGACGGGAATGATGGGAGCA ATTCCACCACCTTATGGATTTTATCTAGCAGATGTAGCAGTAGCTGCAGTCATTGGAATCGTCACCGGCTTGTGTTTGGG TCCAATAATACCAATTTGTGATCGTTGGCTGGCCAAGTCGTCGATCTTGAAATTCTTGCTTCACTTCACTGTAGTCATGT TGGCGGTATCATCACAGTTCTTTCCTTATAGCAAAGATGCACCAAAGAGAGTTGTACTCCAACACACATTCATCTCTACT GGTGGAAATGAAATCACAGGCTCAAGTTATGATTTAGCTGTCATTGATTCAAATTCTATGGAGTTCATTTTTAAGCATGC TCCGGAGGTGGCAAAGGAACTTCATGTTGGCCCTTCTTTTTCATTGGGAAACGCTGAAGCGTCTCCTCAAGAAGCTTGGA TGGCACTTTTCCCTATTTCATGTGTATTAACAACAAACGGGAGGTTCCCTGCTAAAGCCAACAATATTTTAGAACGATAT AGCCAATTCCCACTCCTACAAGCGCATAAACCGCAAACAACATTTGAAAATGGAACTCGCAGAGTTCATCTGGAGCTCTC TCTGGGGTCGTTGGAGGAGATTTGGGTCACGGTTTTGAACATAACCGGACCGTTATCAGGGTGGTCCTTTGCCGATGGCA AACCTCCAGCACCTGAACTCCCACCGGGTGGTCCTCCCTCGTACATATTGAGACTGAGTGGCAACAGCGATGAGAAGTGG AACTTCTGGTTAGAGGCAAGTAGTGAAGAAGAAGTGAGAGTAGATGTAGCTGTTCTTGATCAACGTCTTGATGAAGAAAC TATACATTTGAAGGGTCTTTTCCCAAAATGGTCCGATGTCATTGCCTACACTAGTTTTCTTTCTACTTACTTGTTCTGAT CGTAGACTATTTCATATTCTCCTTTTGATATTTCTAAAACATTAGAGAATAAATAAATCCGTTCTTAACATGGGAAAGTT TTTTTTTTTTTGACATCG .............................................N.................................. .................................N.............................................. ................................................................................ ....................N..............N............N............................... ..................i.............................i............................... .................................................................N.............. ..................................................i............................. ............................N.............N..N.................................. ...................i..............i..................i.............N...N........ N..........................................N.................................... ..............................................N...................N............. .......i........................................................................ .......................................N............................i........... ..........N.....................................i...............N............... ...............................................................................i ................................................................................ ................N.............N..................................N......i....... ............................................................................N..N ........................................................i....................... .........................................................N...................... .............N.................................................................. ..........................i...............N................................N.... ..................................N..........N.......................N.......... ................................N.........................i......N.............. ....N...............................................................N..i........ .............N.................N................................................ ............................................................................N... ....................................N........................................... .......N....................N............................i..................N... .......................N.......................................................N ...................i............................................................ .................................................i.............................. ..........................................................................i..... .....................................................................N.......... ...........................................N..........................N......... ...........................N.......N............................................ .....................................................................N.......... .................. Pos Score Pred ------------------------ 46 0.347 - 114 0.318 - 261 0.217 - 276 0.396 - 289 0.481 - 339 0.867 Yes 369 0.610 Yes 466 0.312 - 531 0.534 Yes 589 0.144 - 603 0.294 - 606 0.245 - 660 0.525 Yes 675 0.831 Yes 694 0.636 Yes 708 0.399 - 712 0.311 - 721 0.461 - 764 0.269 - 847 0.333 - 867 0.471 - 888 0.612 Yes 1000 0.276 - 1029 0.787 Yes 1051 0.128 - 1089 0.787 Yes 1105 0.296 - 1200 0.817 Yes 1297 0.109 - 1311 0.348 - 1346 0.438 - 1353 0.563 Yes 1437 0.290 - 1440 0.235 - 1497 0.502 Yes 1578 0.138 - 1614 0.393 - 1707 0.638 Yes 1723 0.494 - 1756 0.144 - 1795 0.410 - 1806 0.184 - 1830 0.290 - 1873 0.207 - 1899 0.624 Yes 1906 0.099 - 1925 0.460 - 1989 0.480 - 1992 0.762 Yes 2014 0.306 - 2032 0.113 - 2157 0.265 - 2197 0.168 - 2248 0.159 - 2269 0.102 - 2298 0.658 Yes 2317 0.180 - 2344 0.202 - 2400 0.437 - 2420 0.571 Yes 2530 0.519 Yes 2635 0.717 Yes 2710 0.338 - 2764 0.169 - 2791 0.079 - 2828 0.328 - 2836 0.263 - 2950 0.044 -

Explain the output -- Processing locus 52: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGTCTTCGATTCTATATGCGTTAGTAATTATACTCTGTCTAAGCTTTCCTAGAGGTGATAAATCGGTGGATCGTAGCGGC AGTGATCGATTTGAAGTTTGAAATCTAATCGCATGTAACTTTGTTGGATTTATTCTGTTTCTAGGGCTTTTGATTCTAAT GTGGTTGCTTCTATATAACTGCAGAGGAATTGCTGCTCTCTACTCGCGGTGTTTAGTTTACGATTGGAATATGGGGAAGA TATTTAGCTGGTTAGTGGTGTTATTGTCATTGATCTCATTAGTCCCTGTTCCATCGGAATCGGCTGTTTTGAGTGTAGAT CTAGGTTCAGAATGGGTTAAAGTTGCCGTAGTTAATCTGAAACGAGGACAGAGTCCAATCTCTGTAGCCATTAATGAGAT GTCCAAGAGGAAATCCCCAGCTTTAGTAGCATTTCAATCTGGAGACCGGTTACTAGGCGAGGAGGCTGCGGGAATAACGG CTCGTTATCCGAATAAAGTTTATTCACAACTGAGGGATATGGTGGGAAAACCTTTCAAACATGTTAAGGATTTCATTGAT TCTGTCTACTTGCCTTTTGATATTGTAGAAGATTCTAGAGGTGCAGTTGGTATTAAGATCGATGACGGTTCGACTGTTTA CTCAGTGGAGGAGTTGTTAGCTATGATCTTGGGTTATGCCTCAAATCTAGCTGAGTTCCATGCTAAAATCCCAGTGAAGG ATATGGTTGTTTCAGTTCCACCTTACTTTGGACAGGCTGAGAGACGGGGTTTGATCCAGGCTTCTCAGTTGGCTGGGGTT AATGTCCTCTCCCTGGTTAATGAGCATTCCGGTGCTGCTTTGCAATATGGGATTGATAAGGATTTTGCTAATGGGTCGAG ACATGTCATATTTTATGACATGGGTTCAAGTAGTACTTACGCAGCCCTTGTCTATTACTCAGCTTACAGTGAGAAGGAAT ATGGCAAGACTGTTTCTGTCAACCAATTTCAGGTCAAGGATGTTAGATGGGACTTGGGACTTGGAGGACAGAGTATGGAG ATGCGCTTGGTAGAGCACTTTGCAGATGAGTTTAATAAACAGCTCGGTAATGGAGTTGATGTGAGGAAGTTCCCCAAAGC AATGGCTAAATTGAAAAAACAAGTCAAGCGTACAAAGGAAATTTTGAGTGCAAACACTGCGGCTCCAATATCTGTGGAAT CTCTTCATGATGATCGTGACTTCAGGAGCACAATTACCCGTGAGAAGTTTGAGGAACTGTGTAAAGATCTGTGGGAGAGA TCTCTTACACCTTTAAAAGATGTGCTCAAGCATTCAGGTTTGAAGATTGATGATATATCTGCAGTGGAACTAATAGGAGG AGCTACTAGAGTTCCCAAACTACAGAGCACAATCCAGGAATTTATTGGGAAACAACAGTTAGACAAACATCTAGACGCTG ATGAGGCTATTGTCCTCGGCTCAGCACTACATGCTGCTAACTTGAGCGATGGAATTAAATTGAAACGTAGGCTAGGTATA GTTGATGGTTCCCCCTATGGTTTTCTGGTTGAGTTGGAAGGCCCTAATGTTAAGAAAGATGAGAGCACAAAGCAGCAACT CGTACCACGGATGAAAAAGCTACCCAGCAAGATGTTCAGATCCTTTGTCCTTGACAAAGATTTTGACGTGTCACTTGCTT ATGAGTCTGAGGGTATTCTACCACCAGGAACCACCTCCCCTGTGTTTGCACAGTATTCTGTCTCTGGTTTGGCGGATGCA TCTGAGAAATATTCTTCTCGGAATCTATCAGCACCTATCAAGGCAAATCTGCATTTCTCTCTAAGTAGAAGTGGGATTCT CAGTCTAGATCGAGGAGATGCTGTAATTGAAATCACAGAATGGGTAGACGTTCCTAAGAAGAACGTGACTATTGATAGTA ACACAACTACATCAACAGGCAATGCCACTGACGAGAATTCACAAGAAAATAAAGAAGACCTGCAAACCGATGCTGAAAAC AGCACTGCTTCAAATACAACAGCAGAAGAGCCAGCTGTTGCTTCTCTGGGCACAGAAAAAAAGCTGAAGAAGCGGACATT CAGAATACCTCTGAAGGTAGTTGAGAAAACTGTTGGACCTGGAGCACCATTTTCGAAAGAGTCTCTTGCTGAAGCTAAGA TAAAATTAGAAGCCTTGGACAAGAAAGATAGGGAAAGAAGAAGAACGGCTGAGTTAAAAAACAACCTTGAATCTTATATA TATGCTACCAAAGAGAAGCTGGAAACACCCGAGTTTGAAAAGATATCCACCCAAGAAGAGCGCAAGGCGTTTGTTGAAAA GCTTGATGAGGTGCAAGATTGGCTTTACATGGATGGAGAAGATGCTAATGCCACAGAGTTTGAGAAGCGGCTTGACTCAC TTAAAGCCATCGGCAGTCCCATATCTTTCCGATCAGAGGAGCTTACAGCACGACCAGTAGCAATTGAATATGCTAGGAAA TACCTAACTGAACTCAAAGAGATCATAAAAGAATGGGAGACGAACAAAACTTGGCTTCCAAAAGAAAAAATCGACGAGGT CTCAAAGGAAGCAGAGAAAGTAAAAAGCTGGTTAGATAAGAATGTGGCTGAGCAAGAAAAGACTTCTCTGTGGAGCAAAC CGGTGTTCACGTCCACTGAAGTGTACGCTAAAGTATTTACTCTACAAGACAAGGTCACAAAGGTGAATAAGATCCCAAAG CCAAAGCCAAAGATAGAGAAAGTAACCAAGACGGAGAACACAACAAAGGAGGAGGAACAATCAAAATCCTCTGATGAAGC TGCCAAAGAAGAAGAAAGTCACGACGAGCTTTGATTAAGGATATATAATAAATAGAGTTTTCGTTAATTTTTGTGTTTTT AAGAGGGAAAATAGGATTAAAAGTAGGCAGCTGAGATGAGCAAAAGGATTTTA ...............N................................................................ ................................N.............................................N. ......................................................................N......... ................................................................................ ...........i.............................................................i....N. ................................................................................ ......................................N.....................N................... .............................................................N.................. ......................N............N.......................N.................... ..i............................................................................. ..N...........N....i............................................................ i......................................N......N...........................i..... N........................N.......................N........N..................... ......N..N...................................................................... ...................N.............................N.............................. ................................................................................ N.............................N.................N............................... ....N...........N.............................N...........N..................... ..........i....................i................................................ N..........................................................................i.... ................................................................................ .................N.....................N........................................ .....................N...............................................N.......... ................................................................................ ................................................................................ ................................................................................ .....N......................i...i........N.....N................................ .....................................................................N.......... ................................i............................................... .........................................i...................................... ................................................................................ .........................................................................N...... ................................................................................ ...................................N................. Pos Score Pred ------------------------ 16 0.434 - 113 0.168 - 159 0.281 - 231 0.455 - 332 0.503 Yes 394 0.662 Yes 399 0.345 - 519 0.496 - 541 0.103 - 622 0.245 - 663 0.499 - 676 0.233 - 700 0.166 - 723 0.563 Yes 802 0.282 - 820 0.106 - 847 0.256 - 871 0.424 - 883 0.375 - 895 0.338 - 900 0.713 Yes 961 0.741 Yes 1000 0.220 - 1007 0.462 - 1035 0.571 Yes 1041 0.482 - 1066 0.096 - 1090 0.166 - 1099 0.109 - 1122 0.504 Yes 1207 0.117 - 1210 0.164 - 1300 0.393 - 1330 0.205 - 1441 0.085 - 1471 0.112 - 1489 0.365 - 1525 0.226 - 1537 0.222 - 1567 0.158 - 1579 0.206 - 1611 0.514 Yes 1632 0.705 Yes 1681 0.192 - 1756 0.594 Yes 1858 0.230 - 1880 0.438 - 1942 0.217 - 1990 0.148 - 2242 0.120 - 2326 0.081 - 2349 0.709 Yes 2353 0.585 Yes 2362 0.314 - 2368 0.210 - 2470 0.348 - 2513 0.831 Yes 2602 0.546 Yes 2794 0.200 - 2916 0.070 -

Explain the output -- Processing locus 53: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTTAGTCTTAGGTAATGTAACCTCAAGAACTCTATGCGAGAACATTACACATTATCTTTGGAATTGGATATTATATTG TTAAAGTTTAATCATTGATGAAAGTTGCAAAGAGTTATGATGACTCCGTTTTGGGATTGTCTTACCGTTGTCTTTCTGGT TTTAATGCTTAGGTTTCTCTTGATGAAGTCTAGAAGTGAAAGTAAGAATTGTAGGCTTGCACAAGCCTCTAGAGTAGCAG TCAACAAAGTTCTAGACAAAAGCAGCGCCCGTGGCTCTCGAGGCAAGAAGAAGCAAGATGATAACTGTGATTCCGCTAAG CGGGATAAAGGCGTAAACGGTAAGGGAAAGCAAGCGCTCGACGCAAGATTGATTGATAACGTTCTAGAGGACAGGGGATG TGGTAATGTTGATGATGATGAAATGAATGATTCAGACTGGGAAGACTGTCCAATCCCTAGTCTTGATAGTACGGTTGATG ATAATAATGTTGATGACACCAGAGAGCTAACCATAGAATTTGATGATGATGTTCCTGATGCTAAGAAGCAGAAGAATGCA TACCGTGCAACAGCAGAAGACAAGGTACGGGCGGAACTTGTCCATAAGGTGCACTTGCTCTGTCTGCTTGCAAGAGGGAG GATAGTTGACAGTGCTTGTAATGATCCCTTGATTCAGGCTGCCTTGCTTTCACTTCTACCATCATACTTGACAAAAGTAT CAAATCTCGAGAAAGTTACTGTCAAGGATATAGCCCCTCTTCTTCGTTGGGTTCGTGAAAACTTTTCTGTTAGTTGTTCT CCCAGTTCTGAGAAATCTTTTCGTACATCCCTAGCATTTGCTCTTGAATCTCGTAAAGGCACAGCGGAAGAGCTTGCAGC ACTGGCTGTTGCTTTGCTTAGAGCATTAAAGCTCACAACCCGGTTTGTGTCTATTCTTGATGTTGCATCCTTAAAACCTG GGGCCGATAGGAATGAATCATCAGGTCAAAACAGAGCTAAAATGAAGCATGGGATATTCAGGACGTCAACACTTATGGTA CCAAAACAGCAGGCCATCTCGTCATACCCGAAGAAATCTTCCTCCCATGTTAAGAATAAAAGTCCCTTTGAAAAGCCTCA GCTTGGGAATCCCCTGGGCTCTGATCAGGTACAAGACAATGCAGTCAATTCATCTTGTGAAGCAGGAATGTCCATAAAAT CTGACGGAACAAGGAGGAAAGGAGATGTTGAGTTCGAGAGGCAAATAGCCATGGCTTTGTCTGCAACTGCAGACAACCAA CAGAGCTCTCAAGTGAACAATACGAAGAAAGTTCGTGAAATAACAAAAATAAGTAATAGCTCGTCAGTTTCTGACCAAGT AATATCCACGGCTTTCGGTTCTAAGAAAGTAGATTCTCCCCTTTGTTGGCTTGAGGTGTATTGCAATGGGGAAAACATGG ATGGGAAATGGGTCCATGTCGATGCTGTTAATGGAATGATAGATGCAGAGCAAAACATTGAAGCAGCAGCTGCCGCATGC AAAACCGTTCTCAGATATGTTGTTGCATTTGCTGCTGGTGGAGCTAAAGATGTCACTCGCAGGTACTGTACAAAGTGGCA CACTATTTCATCCAAACGGGTGTCTTCAGTGTGGTGGGATATGGTATTAGCACCGTTAGTACATCTCGAGTCAGGTGCAA CTCACGATGAAGACATTGCTCTGAGAAATTTCAATGGCCTAAATCCTGTTTCTAGTAGAGCTTCCTCGAGTAGCTCGTCG TTTGGTATAAGGAGTGCTCTTGAAGATATGGAGTTGGCTACACGGGCGCTGACTGAGTCTCTTCCTACTAACCAGCAGGC TTATAAAAGTCATGAAATATATGCTATTGAGAAATGGCTTCACAAGAACCAGATACTTCACCCAAAAGGTCCAGTTCTGG GATTTTGCTCTGGTCATCCAGTATATCCTCGAACCTGTGTGCAGACTCTTAAAACAAAAGAAAGATGGCTTCGTGATGGG CTACAACTTAAGGCGAATGAAGTTCCCTCAAAGATTCTTAAGCGAAACTCGAAGTTCAAGAAAGTAAAAGATTTTGAAGA TGGCGACAACAACATTAAAGGTGGTTCTTCTTGCATGGAACTATATGGGAAGTGGCAAATGGAACCATTGTGTCTCCCTC CCGCTGTTAATGGAATTGTGCCCAAGAACGAGCGCGGTCAAGTTGATGTCTGGTCTGAGAAATGTCTCCCACCTGGAACA GTCCACTTAAGGTTTCCTCGAATATTTGCGGTTGCTAAGAGATTCGGTATAGATTATGCACCTGCCATGGTTGGTTTCGA GTACAGAAGTGGAGGTGCTACTCCTATTTTTGAAGGTATTGTGGTCTGTACCGAGTTCAAAGACACAATCCTCGAGGCAT ATGCAGAAGAACAAGAAAAGAAAGAAGAAGAGGAGAGAAGAAGAAATGAGGCACAAGCAGCTTCGAGATGGTATCAGCTT CTTTCGTCTATCTTAACCCGTGAAAGATTGAAAAACCGTTACGCTAATAACTCTAACGATGTCGAAGCCAAGAGTTTGGA AGTGAATTCAGAAACAGTCGTTAAGGCAAAGAATGTGAAAGCACCTGAAAAGCAGAGAGTGGCTAAGAGAGGAGAAAAGT CACGAGTAAGAAAGTCTCGTAATGAAGATGAAAGTCATGAACATGTGTTTCTTGATGAGGAAGAAACCTTTGATGAGGAA ACCTCTGTGAAAACGAAACGCTGCAAATGTGGCTTCTCTGTCGAAGTTGAACAAATGTAGTTTTGACTTCGTTTACTGGC CCTAGGAGTCTAGCTAGGAGTTTCATTTGTTTAGAAAATGATTTTACATAAAATTAGGGTGATCTATCTATTATTCCAC ................i..................N............................................ .................N..................N..i........................................ ....N.................i......................................................... .........................................................N...................... .............................................................................N.. .....N.....N..N..N....i...N..................................................N.. ......N.....N.............................N..N..N........N.................N.... ................................................................................ ....................N........................................................... ................................................................................ ................................................................................ ...........................................................N.................... ............N............................i......N.........................N..... ..............................................N................................. ......................................N............................N............ ........................N.........................N............................. ................................................................................ .................................................................i..........i... N......N.......N.....N........N....i......N.................................N... ................N................................N.............................. ........................................i....................................... ......N..........................N.............................................. ...........................i.................................................... ...........N........N............i.............................................. ................................................................i..........N.... ................N..............................................................i ..................................N.........N.............i..................... .........N...................................N...............i.................. .......................................................N..........i............. ................................................................................ N............................................N.....................N............ ..........................................................i..................... ................................N............................................... .....................N.....N........N.....N...........N.................N....... ..........................N...........................i......................... .....................................N......................................... Pos Score Pred ------------------------ 17 0.502 Yes 36 0.161 - 98 0.377 - 117 0.247 - 120 0.636 Yes 165 0.402 - 183 0.507 Yes 298 0.398 - 398 0.109 - 406 0.126 - 412 0.174 - 415 0.180 - 418 0.112 - 423 0.787 Yes 427 0.089 - 478 0.139 - 487 0.276 - 493 0.145 - 523 0.099 - 526 0.164 - 529 0.109 - 538 0.183 - 556 0.184 - 661 0.282 - 940 0.171 - 973 0.387 - 1002 0.673 Yes 1009 0.392 - 1035 0.220 - 1087 0.225 - 1159 0.229 - 1188 0.481 - 1225 0.337 - 1251 0.491 - 1426 0.535 Yes 1437 0.856 Yes 1441 0.311 - 1448 0.309 - 1456 0.198 - 1462 0.152 - 1471 0.126 - 1476 0.705 Yes 1483 0.217 - 1517 0.197 - 1537 0.329 - 1570 0.359 - 1641 0.501 Yes 1687 0.302 - 1714 0.409 - 1788 0.695 Yes 1852 0.328 - 1861 0.187 - 1874 0.573 Yes 1985 0.745 Yes 1996 0.425 - 2017 0.184 - 2080 0.574 Yes 2115 0.388 - 2125 0.205 - 2139 0.721 Yes 2170 0.295 - 2206 0.253 - 2222 0.557 Yes 2296 0.341 - 2307 0.798 Yes 2401 0.233 - 2446 0.482 - 2468 0.181 - 2539 0.512 Yes 2593 0.270 - 2662 0.454 - 2668 0.148 - 2677 0.110 - 2683 0.150 - 2695 0.237 - 2713 0.128 - 2747 0.243 - 2775 0.540 Yes 2838 0.071 -

Explain the output -- Processing locus 54: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 55: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCATATAATAATAAAAACTTGATATTGTTCTGACTTTGGGGTGTCAAATTCTATTTATAAGAATTTGAGATGCAAATC TCAATTTTAGTTACTTTATGATACCAATATAAAAAAAAAAACCACTCTTTCGAAAGGATGCGGGTTTTGAAATACTTTCG AGCTCCTTCTTCTCTCCGTCATCGTTCCTCTCCCATGGCTCAGTTTCTTCGACGCTTCTCTTCCTCTCCCATGGCGAACG AAGACGAATTCCAATCTCCGGTGGAACCATCACAGCAGCAATCTCAGGACTGTGTTTCGGAGTCTTATCTGATAGGGTTT GTTATCGCGAACATAGTAGGGTTAAAGTACTATTCCGGCCGAATCAACGGTCGAGAGATGGTGGGTCTGGTTCGAGAACC CTTGAACGTATATGACAATAATGCAATCAGAGTACTCAACACGCGATCTGAGCAAGTGGGTCACATCGAGCGTACAGTAG CAGCCGTTCTCGCGCCGATGATTGATTCTCACACGATCGTCGTCGAGGGTATTGTACCCAATACTCGTTCTAACTCCAAT AGGTACAGAATCCCTTGTCAAATTCATGTATTTGCAAAGTTAGAAGCGTCCTCTACTGTGAAATCGACGATTTCTAGAGG TGGGTTAGTATTGATTTCGGAGTCAGATACATCGTTTGGGTTATCTGAGGCTGTTGTGGTTAAGGAACAGATGGGAAATG GAGATAAGAGGAGTGTTGATAAGATTTTTAAACTTGTTGATGAGAATGTGAAGTTAATGGGGAAATTGGTAGCGGCTGAG CCACCTAGGGAAGTGATAAAGTCTGAACTTTTTGCTCATCAGAAGGAAGGATTAGGCTGGTTACTCCATAGAGAGAAATC TGGAGAATTGCCTCCGTTTTGGGAGGAGAAAGATGGAGAATTTTTGAATACTTTGACGAATTACCGCTCGGATAAGCGGC CTGATCCTTTGCGTGGAGGGGTGTTTGCTGATGATATGGGGTTGGGTAAGACCCTTACTTTGCTTTCCCTGATAGCTTTT GATAGATATGGCAATGCATCCACCAGTACGCCAACCGAAGAGCCTTTGGATGGTGAAGGAGACAAGATTGAAAAGAAGGG TAAAAAGAGAGGAAGAGGCAAGAGCAGTGAAAGTGTAACTCGAAAGAAGCTTAAAACTGATGATGTTGTTGGCATGAATG TGTCTCAAAAGACAACGTTGATCGTTTGTCCACCTTCTGTGATTTCAGCATGGATAACACAGTTAGAGGAGCATACTGTA CCGGGAATCTTGAAGGTGTATATGTATCATGGTGGCGAAAGGACAGACGATGTTAATGAGCTTATGAAGTATGACATAGT GTTGACTACTTACGGCACTTTAGCTGTTGAGGAATCGTGGGAAGATTCTCCTGTAAAGAAAATGGAGTGGCTGAGAATAA TTCTTGATGAGGCACACACCATTAAGAATGCAAATGCTCAACAGAGCCGAGTAGTTTGTAAGTTGAAGGCTTCCCGCAGA TGGGCTGTCACAGGAACTCCTATTCAGAATGGTTCATTTGATTTGTACTCTTTGATGGCCTTTCTCCGGTTTGAGCCATT TTCGATTAAGAGTTATTGGCAAAGTTTGATACAACGCCCGCTTGGCCAAGGGAATAAAAAGGGTCTTTCACGTCTGCAGG TTTTAATGGCGACAATTTCGTTAAGAAGAACAAAAGAAAAGAGTTTGATTGGTTTACCGCCTAAGACTGTTGAAACTTGT TATGTTGAGCTTTCTCCGGAAGAACGTCAGTTATATGATCACATGGAAGGAGAAGCGAAAGGTGTTGTGCAGAATCTTAT AAATAATGGAAGCTTGATGCGCAACTACTCAACAGTTTTGAGTATAATCTTACGGCTTAGACAGCTTTGCGATGACATGT CTCTATGCCCTCCAGAATTAAGATCTTTTACCACTTCTACATCTGTAGAAGATGTGACTGATAAGCCAGAGCTACTGCAA AAGCTGGTTGCAGCATTGCAAGATGGCGAAGATTTTGACTGTCCAATCTGCATTTCGCCACCAACAAACATCATCATCAC TCGATGTGCCCACATCTTTTGCAGGGCTTGTATCCTTCAAACTCTTCAAAGAAGTAAACCATTGTGCCCTCTTTGCCGTG GCTCCCTTACCCAATCTGATCTCTACAATGCTCCGCCTCCTCCTCCGGATTCTTCCAATACCGATGGAGAAGACGCTAAA TCTTCAACCAAATCCTCAAAGGTCTCAGCTTTACTATCCCTGCTCATGGCATCGAGACAAGAAAACCCCAACACAAAGTC TGTTGTGTTCTCTCAATTCAGGAAGATGCTGCTTTTACTAGAAACTCCACTAAAAGCTGCGGGTTTCACCATCTTGCGCT TGGATGGAGCAATGACTGTGAAGAAACGGACCCAAGTCATTGGAGAATTTGGGAATCCAGAGTTAACCGGACCTGTGGTG TTGCTTGCGAGCCTCAAGGCCTCTGGTACTGGCATAAACCTAACTGCAGCCTCAAGGGTTTACTTGTTTGACCCGTGGTG GAACCCTGCAGTTGAAGAACAGGCCATGGACAGGATTCATAGGATCGGGCAGAAACAAGAGGTTAAAATGATAAGGATGA TTGCGAGAAACAGTATCGAAGAGAGGGTCCTTGAGCTTCAGCAGAAGAAGAAGAATCTAGCAAACGAAGCTTTTAAAAGA AGGCAAAAGAAAGATGAAAGAGAGGTTAACGTTGAAGACGTGGTTGCTCTCATGTCTCTCTGAAACTATCAATCATTGCT TAGCTCCTTAACCGTTTTTTTAAGATACATTAACCGGTAATTTACTGCTTTTGGTACGTAACCTCAACCGATGTTTTTGT TG .......................................................................N........ .................N.......................................i...................... ..................................i...................................i......... ................................................................................ .........................................................i...................... ...........N........N........................................................... .................i.............................................................. .........................N...................................................... ......................................................................i......N.. .......................................N.....N..........i....................... ................................................................................ ................................i............................................... ..............................N....i............................................ .......N.....i...................................N.............................. ...........................................................N..N..........N...N.. .................................................i.............................. .....................N......N....................N.....N.......N......N......... .............................................................i.................. ......N....................N.....N.............................................N ............................N.........................N......................... ................................................................................ .....N.......................................................................... .....N..........N......................................................N....i... ....N..............................................N............................ ......................i......................................................... ...N............................................................................ ...........................N...................................N................ .............................................i.................................. .........................N...................................................... ...N.......i.................................................................... ................................................................................ .........................i.........................................i........i... ................................................................................ .............N.....................................N............................ ......................................................................N......... .. Pos Score Pred ------------------------ 72 0.324 - 98 0.096 - 138 0.555 Yes 195 0.550 Yes 231 0.771 Yes 378 0.785 Yes 412 0.174 - 421 0.313 - 498 0.588 Yes 586 0.180 - 711 0.622 Yes 718 0.138 - 760 0.174 - 766 0.253 - 777 0.700 Yes 913 0.639 Yes 991 0.154 - 996 0.642 Yes 1048 0.377 - 1054 0.602 Yes 1090 0.239 - 1180 0.155 - 1183 0.128 - 1194 0.241 - 1198 0.172 - 1250 0.535 Yes 1302 0.490 - 1309 0.360 - 1330 0.195 - 1336 0.104 - 1344 0.255 - 1351 0.196 - 1422 0.812 Yes 1447 0.359 - 1468 0.339 - 1474 0.208 - 1520 0.446 - 1549 0.160 - 1575 0.444 - 1686 0.459 - 1762 0.253 - 1795 0.107 - 1803 0.581 Yes 1846 0.467 - 1857 0.342 - 1912 0.464 - 1917 0.595 Yes 1925 0.347 - 1972 0.229 - 2023 0.781 Yes 2084 0.236 - 2188 0.280 - 2224 0.343 - 2286 0.685 Yes 2346 0.487 - 2404 0.341 - 2412 0.805 Yes 2586 0.650 Yes 2628 0.658 Yes 2637 0.646 Yes 2734 0.164 - 2772 0.220 - 2871 0.048 -

Explain the output -- Processing locus 56: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTGCTTTAGACAGAATCTATTTTATCCAATTTTTGCTTTACTATATATATAGATACGTATATGTGTTCTTAAACTTGC ACAAACATTTCTGCAATCTAAACCTCAATCAAAACAAACAAACAAAAAACCATGATGCAGCGAGTATTCACCTTTGGAAA AGGAAGAAGCGAAGGCAACAAGGGCATGAAGTCCTTGTTGGGAGGGAAAGGAGCCAACCTGGCGGAGATGGCTAGCATAG GCTTGTCGGTGCCGCCGGGGCTAACCATATCGACGGAGGCTTGTCAGCAGTATCAGATCGCCGGCAAAAAGCTTCCAGAA GGTTTATGGGAAGAGATCTTAGAAGGTCTTAGCTTCATCGAACGTGACATTGGAGCTTCCCTCGCTGATCCCTCCAAGCC ACTCCTCCTCTCTGTTCGCTCCGGCGCCGCCATCTCAATGCCTGGTATGATGGACACTGTACTTAACCTTGGCTTGAACG ACCAAGTCGTCGTTGGTCTGGCCGCAAAAAGCGGAGAGCGTTTTGCTTACGATTCGTTCCGGCGTTTTCTTGATATGTTT GGTGATGTTGTGATGGGAATTCCACACGCCAAGTTTGAAGAGAAGTTAGAGAGAATGAAGGAGAGGAAAGGAGTTAAAAA TGACACTGACTTAAGCGCGGCTGATCTCAAGGAATTGGTTGAGCAGTACAAGAGTGTTTACTTAGAGGCCAAGGGTCAAG AGTTTCCTTCAGATCCAAAGAAGCAATTGGAGCTAGCGATTGAAGCGGTATTCGATTCTTGGGATAGCCCGAGAGCGAAC AAGTACAGAAGTATTAACCAGATAACTGGATTGAAAGGAACCGCGGTTAACATTCAGTGTATGGTGTTTGGAAACATGGG GGACACTTCAGGGACTGGTGTTCTCTTCACTAGGAACCCTAGCACAGGAGAGAAGAAGCTTTATGGCGAGTTTCTAGTTA ATGCTCAGGGAGAGGATGTGGTTGCAGGGATAAGAACACCAGAAGATTTGGATACAATGAAGAGATTTATGCCTGAGGCT TACGCTGAACTTGTTGAGAACTGCAACATCTTAGAAAGACATTACAAAGACATGATGGATATTGAATTCACAGTACAAGA AGAGAGATTGTGGATGCTGCAATGCAGAGCGGGTAAGCGAACGGGTAAAGGCGCCGTGAAGATAGCAGTTGATATGGTAG GTGAAGGGCTTGTTGAGAAATCTTCTGCTATCAAAATGGTGGAGCCTCAACATCTTGATCAACTACTTCACCCACAGTTT CATGATCCATCGGGGTATCGTGAAAAAGTGGTGGCCAAAGGCTTACCTGCGTCACCAGGAGCGGCGGTTGGACAGGTTGT GTTCACGGCGGAGGAAGCCGAAGCTTGGCATTCTCAGGGTAAAACTGTGATTCTGGTTCGAACTGAGACAAGCCCTGACG ATGTGGGAGGTATGCACGCAGCGGAAGGTATATTGACGGCTAGAGGAGGAATGACGTCACACGCGGCTGTTGTTGCTCGC GGTTGGGGAAAATGTTGCATTGCTGGTTGTTCCGAGATTCGTGTCGACGAGAACCACAAGGTTCTATTGATTGGAGATTT GACGATTAATGAAGGCGAATGGATCTCAATGAACGGATCAACCGGTGAGGTTATATTAGGGAAACAAGCATTGGCTCCTC CGGCTTTAAGTCCAGATTTGGAGACTTTCATGTCCTGGGCTGATGCAATCAGACGTCTCAAGGTTATGGCGAATGCGGAT ACACCTGAAGACGCCATTGCAGCTAGGAAAAACGGAGCTCAAGGAATCGGGCTTTGTAGGACAGAGCATATGTTCTTTGG AGCAGATAGGATTAAAGCAGTGAGAAAGATGATAATGGCGGTAACAACAGAGCAAAGGAAAGCTTCTCTCGACATCTTGC TTCCTTACCAACGTTCGGATTTCGAAGGGATCTTCCGTGCTATGGATGGTTTACCGGTAACAATCCGTTTGTTAGACCCT CCGCTTCACGAGTTTCTCCCGGAAGGCGACTTGGACAACATTGTACATGAGCTAGCTGAAGAAACTGGTGTGAAAGAAGA TGAAGTCTTGTCACGGATAGAGAAACTCTCTGAAGTGAATCCAATGCTTGGTTTCCGCGGTTGCAGGCTCGGAATATCGT ATCCAGAGCTAACGGAGATGCAAGCGCGTGCAATTTTTGAAGCTGCAGCGTCAATGCAGGACCAAGGTGTTACTGTCATT CCTGAGATTATGGTTCCACTTGTAGGAACTCCTCAGGAATTGGGTCACCAAGTTGATGTAATTCGTAAAGTTGCAAAGAA AGTATTTGCTGAGAAGGGTCATACCGTGAGCTACAAGGTTGGGACAATGATTGAGATCCCTCGAGCCGCGCTCATTGCAG ATGAGATTGCGAAAGAGGCGGAGTTTTTCTCGTTCGGGACAAACGACTTGACGCAGATGACGTTTGGATACAGTAGAGAC GATGTCGGCAAGTTTCTACCGATTTACCTCGCCAAAGGAATCTTACAGCACGACCCTTTTGAGGTTCTTGATCAGCAAGG TGTAGGGCAATTGATCAAGATGGCGACAGAAAAAGGACGAGCAGCTAGGCCTAGCCTCAAGGTTGGGATATGTGGAGAAC ATGGAGGAGATCCATCTTCTGTGGGATTCTTTGCTGAAGCAGGACTTGACTATGTCTCTTGTTCTCCTTTCAGGGTTCCA ATTGCAAGGCTTGCAGCTGCTCAAGTAGTTGTTGCATGATGAAGAAAAAACTTGGTGGTGTTATTCTGTGAATAAAAAAG CTTTGGTTTCTATTCCATAAAAATGAACAAAATCAAAGATATTGGATTTAACTATTTT ..............................................................N................. ...................................................i..i......................... .........................i.........................................i............ ................................................................................ .....N.......................................................................... .....................................i........i..i.............................. ..........................................................................i..... ....N.......i.........................................i........................N ................................................................................ ................................................................................ ............................................................N..............i.... ..............................................................N................. N..............N........................................i...........N........... ...................................................i..i......................... .............i.......N...................................................N...... ...................................i............................................ ................................................................................ N..........i......................................i............................. ...........i.................................................................... ........N.........i.........i................................................... .............................i............N......................i......N....... .....................................................................N.......... ............................N.....i............................................. .........................................i...N.................................. ..............................................N................................N ...........................................N.................................... .................i...................................i.......................... .........i.............................................N........................ ..............................................i................................. N.......................................................N....................... ...................i.................................................N.......... N..................................................N............................ ...................................N..N......................................... ......................N................................... Pos Score Pred ------------------------ 63 0.359 - 132 0.670 Yes 135 0.714 Yes 186 0.589 Yes 228 0.659 Yes 326 0.463 - 438 0.778 Yes 447 0.756 Yes 450 0.640 Yes 555 0.692 Yes 565 0.106 - 573 0.505 Yes 615 0.706 Yes 640 0.415 - 861 0.213 - 876 0.818 Yes 943 0.258 - 961 0.176 - 976 0.264 - 1017 0.647 Yes 1029 0.478 - 1092 0.608 Yes 1095 0.776 Yes 1134 0.709 Yes 1142 0.403 - 1194 0.417 - 1236 0.601 Yes 1282 0.203 - 1441 0.264 - 1452 0.789 Yes 1491 0.775 Yes 1532 0.545 Yes 1609 0.196 - 1619 0.577 Yes 1629 0.659 Yes 1710 0.677 Yes 1723 0.354 - 1746 0.771 Yes 1753 0.216 - 1830 0.401 - 1869 0.361 - 1875 0.783 Yes 1962 0.664 Yes 1966 0.343 - 2047 0.369 - 2080 0.329 - 2124 0.358 - 2178 0.707 Yes 2214 0.663 Yes 2250 0.711 Yes 2296 0.062 - 2367 0.789 Yes 2401 0.139 - 2457 0.274 - 2482 0.317 - 2580 0.808 Yes 2630 0.202 - 2641 0.298 - 2692 0.253 - 2756 0.175 - 2759 0.151 - 2823 0.083 -

Explain the output -- Processing locus 57: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCAATGGTCGTTAATGCCTGTTCGATCAGATTTTTTTCTAACTCCGAATCATCAAGTGGGTATATAATGAGCCCGTAA AAATCACTCCTTTTCGATTAAAAGATGGGATTTTTCGTGATGATTAGAGATGTTTCCATGGGATTTATGCTCCTATGTAT TTCTGCTTTGTGGGTTTTGCCAATACAAGGTGCTGGTAGAGAAAGTTTCTCAAGAAACTCTTCTTCTTCTTCTCTGCCAA GCTCTGTAAACGTTGGAGCTCTGTTTACTTATGATTCTTTCATTGGAAGAGCGGCCAAACTTGCGTTTGTGGCGGCCATT GAAGACATTAATGCTGACCAGAGTATCCTCAGGGGCACCAAGCTTAATATTGTCTTCCAGGACACTAATTGCAGTGGATT TGTTGGCACCATGGGAGCTTTGCAGCTAATGGAGAACAAGGTGGTTGCAGCCATTGGTCCACAATCTTCAGGAATTGGTC ACATAATCTCCCATGTAGCTAATGAGCTTCATGTACCTTTCTTGTCATTTGCAGCAACGGACCCGACTCTTTCTTCGCTT CAATACCCTTATTTCCTTCGTACCACACAGAATGATTACTTCCAGATGAATGCAATCACGGATTTTGTATCCTATTTTCG ATGGAGAGAAGTTGTTGCGATCTTTGTGGATGATGAGTATGGTAGGAATGGAATATCTGTATTAGGTGATGCTTTAGCCA AGAAACGTGCCAAGATCTCTTACAAGGCTGCATTTCCACCTGGTGCAGATAATAGCTCAATCAGTGATTTATTGGCTTCT GTTAATCTGATGGAATCTCGCATCTTTGTTGTTCATGTGAATCCTGATTCGGGTTTAAACATATTCTCTGTCGCCAAATC TCTTGGAATGATGGGAAGTGGCTATGTCTGGATCACTACTGATTGGCTTCTTACAGCTTTGGATTCCATGGAACCGTTGG ATCCCAGAGCTTTGGATCTCTTGCAAGGAGTGGTTGCATTTCGTCATTACACACCTGAGAGTGACAACAAGAGACAGTTT AAAGGAAGATGGAAAAACCTTAGATTCAAGGAGAGTCTAAAAAGTGATGATGGCTTCAATTCTTACGCGCTGTATGCTTA CGATTCTGTTTGGTTGGTAGCTCGCGCTCTCGATGTTTTCTTCAGCCAAGGCAATACAGTGACTTTCTCTAATGATCCAA GTCTGAGGAATACCAACGATAGCGGCATTAAGCTATCAAAACTTCACATTTTCAATGAAGGGGAGAGGTTCTTGCAGGTC ATTCTTGAGATGAATTATACAGGTCTGACTGGACAAATCGAGTTTAATTCAGAGAAAAACCGGATTAATCCAGCCTACGA CATTCTGAACATAAAAAGTACAGGTCCACTGAGAGTTGGATACTGGTCGAATCATACAGGTTTCTCAGTCGCGCCTCCAG AGACATTATACTCTAAGCCTTCAAACACATCTGCAAAAGACCAACGTCTTAATGAGATCATATGGCCAGGGGAAGTAATA AAGCCACCTCGGGGTTGGGTTTTCCCTGAAAATGGAAAGCCGCTGAAAATCGGGGTGCCTAACCGTGTAAGCTACAAAAA CTATGCTTCTAAGGACAAGAACCCGCTTGGTGTTAAAGGCTTTTGCATCGACATCTTTGAAGCTGCGATTCAATTGCTTC CATATCCCGTTCCACGTACTTATATACTATATGGGGACGGGAAGAAAAATCCTTCATATGACAATCTCATAAGTGAAGTT GCTGCAAATATTTTCGATGTAGCTGTTGGGGATGTTACCATCATTACAAACAGAACCAAGTTTGTAGATTTCACGCAGCC ATTTATAGAATCAGGGCTTGTGGTGGTAGCTCCAGTGAAGGGGGCCAAGTCTAGTCCTTGGTCCTTCTTGAAGCCATTCA CTATAGAGATGTGGGCTGTGACCGGTGCCCTTTTTCTCTTTGTTGGAGCCGTCATCTGGATTCTTGAACATCGATTTAAC GAAGAATTCCGCGGACCTCCTAGGCGTCAAATCATTACAGTCTTCTGGTTTAGCTTCTCAACAATGTTCTTCTCTCACAG GGAGAATACGGTGAGCACGTTGGGAAGGTTTGTGCTACTCGTATGGTTATTTGTGGTTCTAATCATCAACTCAAGCTACA CAGCCAGTCTCACTTCAATCCTCACCGTTCAACAGCTAACATCTCGGATAGAAGGAATGGACACTTTAATAGCAAGCAAC GAACCCATTGGAGTCCAAGATGGTACCTTTGCGTGGAAATTTCTGGTCAATGAACTTAACATAGCTCCATCAAGAATCAT TCCGCTTAAAGACGAAGAAGAATATCTCTCTGCTCTTCAACGTGGTCCCAGAGGCGGTGGCGTGGCAGCCATTGTCGACG AGCTTCCTTACATTAAAGCTCTCTTGTCAAACAGCAACTGCAAGTTTCGAACAGTTGGACAGGAATTCACTCGGACAGGC TGGGGATTTGCGTTCCAGAGAGACTCTCCTCTAGCTGTGGACATGTCGACAGCGATCCTGCAACTGGCTGAAGAAGGAAA ACTCGAGAAAATCCGCAAGAAATGGCTTACCTACGACCACGAATGTACAATGCAGATTTCAGATACAGAAAACTATCAAA TATCGGTACAGAGTTTCTGGGGACTCTTCCTAATATGTGGCGTCGTTTGGTTCATTGCACTCACACTCTTCTGCTGGAAA GTTTTCTGGCAATACCAACGGTTAAGACCAGAAGAGAGTGATGAAGTACAGGCGAGGAGCGAGGAGGCTGGTTCTTCTAG AGGGAAAAGTTTGAGAGCAGTGAGTTTCAAGGATTTGATCAAAGTTGTTGATAAGAGAGAAGCAGAGATTAAGGAGATGC TTAAGGAGAAGAGCAGTAAGAAACTCAAAGATGGCCAAAGTTCAGCTGAGAATTCGCAGTCGAAAGATCACGAAACTCCA CAGTGAGATATATAATAACGAGTTACACAATTTGTTTAATGACTGCAGATATAACATTACACTTAGAAAGGTACATAGAT TTATTACATAGATAGAGATAAAAAT .....N.........N....................................................N........... ........................N..............N.........N.......N........N.......N..... ................................................................................ ..............................N................................................. ..........N..................................................................... ..........i.................N................................................... ............N........N........N................................................. ...............................N.............i...N.............................. N............................N..N.....N........N....................N........... ................................................................................ .........i........................N............................................. .......i..i............N...........................................N............ ................................................................................ ........N.....................................N..N.......................N...... ................................N......................................N........ ......................................................N......................... .........N...................................................................... ................................................................................ ...................................................N.........i.................. ...............................N................................................ ..N............................................................................. ..............................N..........................N...................... ................N..............N................................................ ................................................................................ ........i....................................................................... ...............................................................N................ ..........................................i..................................... ........................................................i....................... ...................N.............................N.............................. ................................................................................ ................................................................................ ..........................................i..................................... .....................N....................N......N.............................. ..................................N............................................. ........................................i....................................... ............................................................................N... ..............................i................................................. ......................................N......................................... ......................... Pos Score Pred ------------------------ 6 0.377 - 16 0.449 - 69 0.319 - 105 0.477 - 120 0.450 - 130 0.237 - 138 0.316 - 147 0.157 - 155 0.217 - 271 0.095 - 331 0.163 - 411 0.517 Yes 429 0.403 - 493 0.242 - 502 0.279 - 511 0.164 - 592 0.474 - 606 0.744 Yes 610 0.162 - 641 0.293 - 670 0.264 - 673 0.133 - 679 0.239 - 688 0.260 - 709 0.171 - 810 0.693 Yes 835 0.202 - 888 0.575 Yes 891 0.564 Yes 904 0.262 - 948 0.424 - 1049 0.403 - 1087 0.359 - 1090 0.425 - 1114 0.167 - 1153 0.115 - 1192 0.452 - 1255 0.424 - 1290 0.494 - 1492 0.280 - 1502 0.582 Yes 1552 0.412 - 1603 0.198 - 1711 0.443 - 1738 0.183 - 1777 0.200 - 1792 0.121 - 1929 0.681 Yes 2064 0.223 - 2123 0.669 Yes 2217 0.703 Yes 2260 0.288 - 2290 0.168 - 2523 0.522 Yes 2582 0.450 - 2603 0.444 - 2610 0.146 - 2675 0.378 - 2761 0.526 Yes 2877 0.459 - 2911 0.726 Yes 2999 0.046 -

Explain the output -- Processing locus 58: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATCGTTGAAAAATCAATTAGAACTAAGAATAAAACAAAAGGAAAATAACTTTATACCAAAAAATGAAGAATCATGGGCC ATGACACATCACCATAAATGACCAAACCCGATTAAGGGGTTTGCTATATTCAAACAAAGCGAACCTAATCTTGTTGAAAT TAATGCCTCCAATCTTCAAATTATACAAACACTTTTAAAGATGATAAAATCACATATATTTCTAGAAAGAAGACACACTA TAAATAAAAACATCCTTGCTGGCAAAAATCCATCATTCAATTTTGGTCGGTCAAATAGATCGTCACAAAATGTTATAAAG CATTATCACATCTTTAAATTCATTCACATTTCGATATCTTTACACATTCACATGGCGCCAATGGCTATGGATCATCTCTG TTTCGTACTACCGACAGAATCCGGCGAGTTAAAGCCACCGGTGATGGTCGAGGAGACCACAGAAGAAGAAGAGGAGAAGA AGAGTCGTGATTGTGGACGTCAAGTTGTGAGTTTAATCGGAGATTTATTCCGGCGATTACACGGCTCTAAGTTGGTGAAG AGCTTGAATCTCTGTAGTATCAACGAGAGTAAAGATTCGATTTCAATGGAGATCAATAAATCATTCACTGACATGGAAGG TGTTCAGCTTTCCAGCAAAGTCGGTTGCGAGAATCCAAGAATCTTTGGATACTCTGAGCTTTATATCGGAACTAATGGTT TCAGCGACGAACTGATCCTCGGAAGCGGCGGTTTCGGCCGCGTTTACAAGGCGTTGTTACCGAGCGACGGAACCACCGTG GCTGTTAAATGCTTGGCGGAGAAGAAAGGAGAACAATTCGAGAAAACTTTCGCGGCGGAGCTTGTTGCGGTGGCTCAGCT CCGCCATCGAAATCTTGTGAAGTTAAGAGGTTGGTGTCTACACGAAGACGAGTTGCTTCTTGTTTACGATTACATGCCTA ACCGGAGCTTAGATCGTGTGCTTTTCCGACGACCGGAAGTTAACTCCGATTTTAAACCGTTGGATTGGGATCGGAGGGGT AAAATCGTCAAAGGACTCGCCGCCGCGTTGTTCTACCTCCACGAGCAGTTAGAGACTCAGATCATTCACAGAGATGTGAA GACGAGTAACGTTATGTTGGATTCTGAGTTTAACGCTAAACTCGGAGATTTTGGTTTAGCTCGGTGGTTAGAACACAAGA TTGATGAAACAGAGCATGATTCGAGTTACGACTCTGTTTCTTCCTTCCGTAACCACCAGTTCCGAGTCGCGGATTCGACT CGGATCGGTGGGACTATCGGGTACTTACCACCGGAGAGTTTCCGGAAAAAAACCGTTGCCACCGCTAAAACAGATGTTTT CAGCTTTGGTGTTGTGGTTTTAGAGGTTGTCTCCGGTAGACGAGCCGTTGATCTTTCGTTCTCGGAAGATAAAATCATTT TACTTGATTGGGTTCGGAGATTATCCGATAACCGGAAATTACTCGACGCCGGGGATTCTCGGTTAGCAAAAGGATCTTAT GATCTCTCTGATATGAAGAGAATGATTCATCTTGCTCTGCTCTGTTCTTTAAACAATCCAACGCATCGTCCCAACATGAA ATGGGTTATCGGAGCACTTTCCGGCGAGTTTTCCGGTAACTTACCGGCGTTACCTTCGTTTAAGAGCCATCCTCTTTATA TTCCTTTATCTTCTTTAAAATCAACCTCAACTTCGGCGACTACGACTACTACAAGAACAACGATGACCACCACCACCTCC ACAACTAGCTTCAATGCGTCGTCGGAGTCAACGCCGTCATCAAATTACGTTACTGCCCTTGAGGATTCTATTTACCAGAC GGCGGAGACTGGAGAAAATCCGTACTTTAATTACAACAGCCGGAGAGTGATGTCATCAAAGTCTTTCGTATTGGACACGC CAAGAGAGATTTCATACAACGACCTTGTTTTAGCCACTGATAATTTCTCTGATGCACGTCGCGTTGCTGAGGTTGATTTT GGTACGGCTTATTATGGTTTACTAAATGGAGACCAACATATTGTGGTGAAACGTCTCGGTATGACTAAATGTCCGGCGCT TGTTACCCGGTTCTCCACTGAACTACTTAACCTAGGCCGGCTTCGACACCGTAACCTAGTAATGCTCCGTGGTTGGTGCA CCGAACACGGAGAAATGCTTGTAGTGTACGATTATTCCGCGAATCGTAAGCTAAGTCATCTTCTTTTCCATAATCATATA CCTGGAAACTCTGTTTTGCGATGGAAAAGTCGGTACAACGTGATCAAATCGCTTGCTTGCGCTGTACGTTATCTCCACGA GGAATGGGACGAACAAGTCATTCACCGAAACATTACTTCTTCAACTATATTTCTTGATAGAGATATGAATCCGCGGCTTT GCGGATTCGCATTGGCAGAGTTTTTGTCTAGAAACGACAAGGCTCATCAAGCTGCAAAAAAGAAAGGATCTGCTCAAGGG ATTTTTGGTTATATGGCACCAGAATACATGGAATCCGGCGAGGCTACAACCATGGCGGATGTATACAGCTTTGGCGTGGT AGTGCTCGAGATGGTTACGGGGCAGCCAGCAGTGGATTACAAGAGGAAAAAAGAAGATGCTCTTATGGTGTTGCGAATCC GCGAAGTAGTTGGTAACCGAAAAAAACTCTTGGAGGAGATTGCAGATATTCATTTGGATGATGAATACGAGAATCGAGAA TTGGCAAGGCTATTACGGTTAGGGTTAGTTTGCACGAGAACCGATCCGAAGTTAAGGCCTAGCATTAGCCAAGTGGTGAG TATATTGGATGGAAGCGAGAGGTTCTTCGAAGAGGAAGGAGGAAAGGAAGGTGACGTTAGCCGCAAGCAAATGTATGATT CGTCTATGTTGATGATTAGACAAATGCAAGCGCTTGGGATCCATTGAGTAATGATAAAGGAAATATTAGGAATGTTCATG TAATTTATCTTTGTTGATTATGTTATAAAGATTTTGGGTCTGTTAATTGGTTCCAGGTAAAGCAAG ...............................................................i.........N...... N................N.............................................................. ..N.....................................i....................................... .....................................................................N.......... ...................................................i........i.....i............. ...........................................N.................................... ................................................................................ .............................................i..........................i....... ..........................................................................N..... ................................................................................ ........i....................................................................... .........................................................................N...... ................................................................................ .........................................................................N...... .............N.................................................................. ...N...........i................................................................ .........................................................................N...... ................................................................................ ..............................................................................N. ............N........N.....................................................N.... i............................................................................... ..............................................................i................. .............N.................................................................. .................................................i.............................. ...................................................N............................ .............N...........N..................................i.......i........... .............................................................N.................. ..............i................................................................. ....................N........................................................... ...N............................................................N............... ................................................................................ ............i..............i.......................i......N..................... ..........i.............................................N.......i............... .........................................................N..N................... ................................................................................ ........N.............................................................N...N..... .....N.....N...........N..........................N....................N.....N.. ...................N.............................................. Pos Score Pred ------------------------ 64 0.537 Yes 74 0.454 - 81 0.272 - 98 0.292 - 163 0.330 - 201 0.540 Yes 310 0.124 - 372 0.844 Yes 381 0.835 Yes 387 0.751 Yes 444 0.329 - 606 0.533 Yes 633 0.644 Yes 715 0.362 - 809 0.660 Yes 954 0.456 - 1114 0.418 - 1134 0.484 - 1204 0.413 - 1216 0.601 Yes 1354 0.151 - 1519 0.149 - 1533 0.446 - 1542 0.496 - 1596 0.274 - 1601 0.659 Yes 1743 0.825 Yes 1774 0.389 - 1890 0.729 Yes 1972 0.205 - 2014 0.396 - 2026 0.424 - 2061 0.631 Yes 2069 0.657 Yes 2142 0.449 - 2175 0.674 Yes 2261 0.370 - 2324 0.321 - 2385 0.482 - 2493 0.670 Yes 2508 0.880 Yes 2532 0.765 Yes 2539 0.127 - 2571 0.695 Yes 2617 0.459 - 2625 0.533 Yes 2698 0.208 - 2701 0.151 - 2809 0.372 - 2871 0.184 - 2875 0.063 - 2886 0.112 - 2892 0.118 - 2904 0.114 - 2931 0.123 - 2952 0.118 - 2958 0.036 - 2980 0.053 -

Explain the output -- Processing locus 59: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTGATTTCAAGCTCAACAATCTCAAAATAATCAATTTTTGGTAGTGTGTTAAATCTGAGAAGGGTTTTTGGGTTTTGG GTATAAAGCTTGTTACTTTGATCTTTATTTTGTGAATTCGAAGTGGAAATGGAATCGTTACCATCTCCATTTGGCGATCC AGCTCCTAATTTATCCAATTCGGAGCTCCGAGAAACTGCTTACGAGATCTTAGTCGCTGCTTGCCGTAGCACTGGAAGTC GTCCGCTGACTTACATCCCTCAATCGCCGAAATCTGATCGGAGCAACGGTCTGACGACGGCGTCGTTATCTCCGTCTCCG TCGCTTCATAGATCTCTGACTTCTACAGCTGCGAGTAAGGTGAAGAAGGCGTTAGGGATGAAGAAACGAATCGGAGACGG AGACGGAGGAGCGGGAGAATCGTCGAGTCAACCGGATCGGAGCAAGAAATCCGTCACGGTGGGTGAGTTGGTTCGGGTAC AAATGCGAATCTCGGAGCAGATTGATTCAAGGATTCGTAGAGCTCTTCTCAGGATCGCTTCTGGTCAGCTTGGGAGGCGT GTGGAGATGATGGTGCTACCTCTTGAGCTTCTGCAACAGCTTAAAGCTTCAGATTTTCCAGACCAAGAAGAGTATGAATC ATGGCAGAGAAGGAACTTGAAGCTTCTTGAAGCTGGTTTGATATTATATCCTTGTGTGCCGCTAAGTAAATCAGATAAAT CTGTTCAACAGCTTAAGCAGATTATCCGGTCCGGTCTCGAGAGGCCGTTAGATACCGGGAAGATTACGGGGGAGACGCAG AATCTTAGGAGTTTAGTAATGTCTCTTGCTAGTCGGCAGAACAACAATGGGATTGGTTCTGAGACTTGTCATTGGGCTGA TGGGTTTCCACTGAATCTTAGAATCTATCAGATGCTTTTAGAGTCTTGTTTTGATGTGAACGATGAGTTGCTGATTGTTG AAGAGGTAGATGAAGTTTTGGAGCTTATAAAGAAAACATGGCCTGTCTTGGGGATAAATCAGATGATTCACAATGTTTGC TTTCTCTGGGTGTTGGTTAATCGGTATGTTTCAACCGGGCAAGTGGAGAATGACTTGCTTGTGGCTGCTCATAACTTGAT ACTCGAAATTGAGAACGACGCAATGGAGACAAATGATCCTGAGTATTCCAAGATCTTGAGTTCTGTTTTGAGTTTGGTGA TGGATTGGGGTGAGAAGAGACTTCTTGCTTACCATGATACTTTCAACATTGATAACGTTGAGACGCTGGAAACTACGGTT TCTTTGGGGATTTTGGTAGCTAAGGTACTAGGTGAAGATATTTCTAGCGAATATAGAAGGAAAAAGAAGCATGTCGATTC AGGGCGTGATCGAGTTGATACCTATATCAGGTCTTCGTTGCGTATGGCTTTTCAACAGACAAAGAGGATGGTGGAACATA GCAAGAAATCAAAATCCCGGCAGAGCACGAATAATCTTCCTGCTCTTGCGATTCTTGCAGAGGATATTGGTCATTTAGCT TTTAATGAGAAGGCGATATTTAGTCCAATCTTGAAGAATTGGCACCCGCTTGCAGCTGGTGTGGCTGCAGCTACGCTTCA TTCGTGCTATGGTACCGAGTTGAAGAAATTCGTCTCGGGTATTACTGAGTTGACGCCAGATGCTATTAGAGTACTCACTG CTGCAGATAAGCTTGAGAAGGATCTGGTGCAAATTGCTGTACAAGATGCAGTAGATAGTGAAGATGGTGGAAAATCGGTT ATAAGAGAGATGCCTCCTTTTGAAGCAGAAGTCGTTATTGGTAACCTTGTGAAATCGTGGATCAAGATCCGAGTTGATAG GCTAAAAGAGTGGATCGACCGGAATCTCCAGCAAGAGGTATGGAATCCGAGATCGAATAAACTCGGTATCGCTCCTTCTG CTGTGGATGTACTGAGGATGGTAGATGAGACATTGGAAGCGTTTTTCTTGTTGCCGATTCTTTTGCATCCAGTTTTACTT CCTGAGCTAACGTCAGGACTAGACAAGTGTATGCAGCATTATGTATCAAAGGCGAAATCATCCTGCGGCTCGCGGAATAC GTTTCTACCCGTTTTGCCTGCTTTAACAAGATGCACGGTCGGGTCCAGACTACATGGTGTATTCAAGAAAAAGGAGAAGC CAATGGTAGCTTCTCACAGGAGAAAATCACAGCTGGGGACGGGTAATGACTCGGCGGAAATACTTCAGTTCTGTTGCAGG ATCAACACTCTGCAGTATATCAGGACAGAGATCGAATCATCTGGGAGAAAAACATTGAATCGTCTCCCGGAATCCGAAGT TGCAGCTTTGGATGCTAAAGGTAAAATATTTGAACAGTCGATTAGTTATTGCTCGAAAGGAATACAGCAATTGTCTGAAG CAACTGCTTACAAGATCGTCTTCCACGATCTGAGCAATGTTCTCTGGGATGGTCTGTACCTCGGGGAAGTTCCTTCCTCA AGGATTGAGCCGTTTCTTCAAGAGCTCGAACGGTGCCTTGAGATTATCTCCTCATCGGTTCATGACAGAGTCCGAACCAG AGTCATTTCGGACATCATGAGAGCTTCTTTCGACGGGTTCTTATTGGTCCTCCTTGCAGGTGGACCATCGCGTGGCTTCA CAATTCAAGACTCTGCTGCCGTAGAAGAGGATTTCAAGTTTCTATGCGATCTTTTCTGGTCAAACGGAGACGGATTGCCT TTGGATTTGATCGAGAAGGTTTCGACGACTGTCAAGAGTATACTTCCGCTGCTGCGCACGGATACAGATTCTTTGATTGA ACGGTTTAAAGCAGTGTGTCTGGAGAATCATGGTTCTGACAGAGGGAAGCTTCCGTTACCGCCGACTTCTGGTCCGTGGA GTCCGACAGAACCAAACACACTTTTAAGAGTATTGTGTTACCGTTACGATGAACCTGCGACTAAGTTTCTGAAAAAGACC TATAACTTGCCGAGGAAGCTAACCTGAGAGTGACCTACTTACTACATTGGTACAAAACCCGGTTTAGTTTGAAGTGTGGA ATATATTTTGGTTAAAGCGGCTAGCGATTGGTATTTGGCAAATTAA ................................................................................ ................................................i............................... ................................................................................ ................................................................................ .........................................................N...................... ................................................................................ ..N............................................................................. ......i..N...............................................................N...... i............................................................................... ................................................................................ ..................N...........................N................................N ...............................i.....................N........N................. .........N...........................i........................N.........N....... .........................N.......................N.............................. ......................N.........N..............................................i .................................N.............................................. ......................................................................N......... ...........................................N.......................i............ ................................................................................ ....N........................................................................... ........N..................................................N.................... .............................................N.................N................ .........i...................................................................... .......................................i........................................ ......N..........N......N....................................................... ..............................N.........N....................................... ..............................i......................N.......................... ..i..........................................i.................................. ................................................................................ ...........N.................................................................... ....................................N...........N............................... .............................................................N.................. ................i............................................................... ...........................................i.................................... ................................................................................ .............................i.................................................. ................................................N............................... ................................................................................ .............................................. Pos Score Pred ------------------------ 129 0.588 Yes 378 0.256 - 483 0.486 - 567 0.537 Yes 570 0.246 - 634 0.281 - 641 0.824 Yes 819 0.442 - 847 0.411 - 880 0.121 - 912 0.541 Yes 934 0.154 - 943 0.133 - 970 0.102 - 998 0.713 Yes 1023 0.451 - 1033 0.281 - 1066 0.075 - 1090 0.115 - 1143 0.368 - 1153 0.156 - 1200 0.549 Yes 1234 0.096 - 1351 0.396 - 1404 0.470 - 1428 0.793 Yes 1525 0.186 - 1609 0.048 - 1660 0.149 - 1726 0.234 - 1744 0.142 - 1770 0.713 Yes 1880 0.534 Yes 1927 0.083 - 1938 0.459 - 1945 0.148 - 2031 0.258 - 2041 0.464 - 2111 0.625 Yes 2134 0.321 - 2163 0.556 Yes 2206 0.636 Yes 2332 0.196 - 2437 0.201 - 2449 0.395 - 2542 0.358 - 2577 0.543 Yes 2684 0.612 Yes 2830 0.677 Yes 2929 0.277 -

Explain the output -- Processing locus 60: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTCCATTTAGCATTGCTTCTCCAATTTAATTTGATACAAGATCTTTTGGTTAGATTGCTGCAGTCACAATTTGCTAA TCGAACCGATAGGTTAGTTGACACGTCTGTATAATGTCGTGTTTCCCGCCGTTCTGGACAATCAACAAAAAATGGAAAAA TAGAACCGACCACTTTATAGAACATAGTAACCCTAAACTTAAAATTTTCCAATTATCCTCAATTTTGCGAATTAGGACAG AGATGGAGCTTCGGTCCCGCAATAAGGCGATTCGTCCATCGACTGAAGTTGTGGATCTCGAGGAAGGAACTGGTATCAAC CCTGATGAAGAACCTTATGCTATATCTTCAGATGACGATTCAATCGGCTCTGAATTTCAAGGTGACGAGGAGGAGGAGGA GGAGTTAGAGGAGGTTGTTGCTAATGATGATTTACCAAATCCTGTACCTGTTTTGGCAATTGTAAACCTACCAAGAGCAA GCAAGAAAAGGAAAAAACCTGATGCACGCAAAGAGAAAGTAGTGCTATTGTGGGAGACTTGGGAAAAAGAGCAGAATAGC TGGATTGATGAACATATGTCAGAGGATGTTGATTTAGATCAGCACAATGCTGTGATTGCTGAAACTGCTGAGCCGCCTTC AGATTTGATTATGCCGCTATTAAGGTACCAAAAGGAATTTTTGGCCTGGGCAACAAAACAGGAACAATCTGTTGCAGGAG GGATTCTCGCGGATGAGATGGGAATGGGAAAAACAATACAAGCCATCTCTCTTGTTCTTGCCAGACGAGAAGTTGACAGG GCACAGTTTGGGGAAGCTGCGGGTTGTACTCTGGTTCTTTGTCCTCTTGTTGCTGTTTCTCAGTGGTTAAATGAGATTGC TCGGTTTACATCCCCGGGAAGCACTAAGGTCCTTGTATACCATGGGGCAAAGAGAGCGAAGAATATTAAAGAGTTTATGA ACTACGACTTTGTTTTGACAACATATTCCACTGTTGAGAGTGAATATAGGAGAAACATAATGCCGTCGAAGGTGCAATGT GCATATTGCAGCAAGTCATTTTACCCGAAGAAGCTTGTGATCCACCTGCGATATTTTTGTGGACCTTCGGCTGTCAAAAC GGCAAAACAATCTAAGCAGAAAAGGAAGAAGACTTCAGATTCATCCTCACAGCAAGGCAAGGAGGCTGATGCAGGAGAGG ATAAGAAATTAAAGAAATCTAAGAAGAAGACCAAACAAACAGTAGAAAAAGATCAGTTAGGTTCAGATGACAAAGAGAAG TCTCTTTTGCACTCTGTTAAGTGGAATCGGATCATCTTGGATGAGGCTCATTACATCAAAGAAAGACGTAGCAATACTGC AAGAGCTGTTTTTGCTTTGGAGGCTACCTATAGATGGGCTTTGAGTGGCACTCCTCTCCAAAATCGTGTTGGAGAGCTTT ACTCACTGATCCGCTTCTTGCAAATTCGCCCATACTCGTATTACTTTTGCAAGGATTGTGACTGTAGAATTCTTGATTAC GTTGCTCATCAAAGCTGCCCTCACTGTCCTCATAATGCAGTTCGACATTTCTGTTGGTGGAACAAGTATGTGGCCAAGCC AATAACAGTATATGGAAGCTTTGGACTGGGAAAAAGAGCCATGATATTGCTTAAACACAAAGTTCTGAAGGACATCCTTT TAAGACGTACTAAATTGGGCCGGGCAGCTGATCTTGCTCTTCCTCCTAGAATTATCACTTTGAGGCGGGATACACTAGAT GTAAAGGAGTTTGATTACTATGAATCACTATACAAAAACAGCCAGGCGGAATTTAATACGTATATTGAGGCTGGGACATT AATGAATAACTATGCACATATATTTGATCTTCTCACCCGCCTGAGACAGGCCGTTGATCATCCATACCTTGTGGTGTATT CCAATTCTAGTGGCGCTAATGCTAACTTGGTTGATGAGAACAAAAGTGAGCAAGAATGTGGTCTCTGCCACGATCCGGCC GAGGACTACGTTGTGACTTCTTGTGCACATGTGTTCTGTAAAGCTTGTTTGATTGGTTTCTCCGCGTCCCTGGGAAAAGT CACCTGCCCGACATGCTCGAAACTACTGACTGTGGATTGGACTACCAAAGCTGACACAGAGCATAAGGCGAGCAAGACAA CACTTAAAGGATTTAGAGCCTCAAGCATTTTAAATCGTATAAAGCTTGATGATTTTCAGACAAGTACAAAGATTGAGGCT TTGAGAGAAGAAATTAGGTTCATGGTTGAAAGAGATGGGTCTGCCAAAGCAATAGTTTTCAGCCAGTTCACATCATTTTT GGACCTTATAAATTATACCCTTGGGAAGTGTGGGGTTAGTTGTGTTCAACTGGTGGGAAGCATGACCATGGCAGCTAGAG ATACTGCCATCAACAAATTCAAAGAAGACCCAGATTGCAGAGTTTTCTTAATGAGCTTGAAAGCCGGAGGGGTTGCTCTT AATTTAACTGTTGCTTCACATGTTTTTATGATGGACCCGTGGTGGAACCCAGCGGTTGAGAGGCAAGCACAAGACAGAAT TCATAGGATCGGACAGTACAAGCCAATCAGGGTTGTGAGATTCATAATAGAGAACACAGTGGAGGAAAGGATTTTAAGGC TTCAGAAGAAGAAGGAACTTGTGTTTGAAGGGACGGTTGGTGGCTCTCAGGAGGCTATAGGAAAGCTGACCGAGGAAGAT ATGAGGTTCTTGTTTACAACATAACTTTTTTCTAACAGATGAAAACGCTTTTGTATGACCATGAAGATCTGGTCTCGGTG TAGTAGTATGGTGGTAATGGTATGTGTTACTTAAGCCCGTTTT ................................................................................ .................................i.....................................i........ ................................................................................ ..i............................................................................. ....N...........N..............N................................................ .......................N..N..................................................... .....................N.......................................................... .......N.......i.........N....................N................................. ..........i..................................................................... ............N....i.....i........................................................ ......................................................................N......... .........................................N..................................N... ...........................................................i................i... ................................................................................ ....................................................................N........... ..................................................................N............. ........................................N....................................... .................................N.............................................. ................................................................................ ..................................N................................N............ ...........N............................i....................................... ..............................................................................N. ...................N............................................................ ..................N..............N.....................i........................ ............................N................................................... ............N................................................................... ................................................N............................... .....................N............N............................................. .............................................................i.....i............ ..................................................i............................. ...................N.......N..N................................................. ................................................................................ ................................................................................ N.....................................N...............N.....N................... .......N........N....N..................... Pos Score Pred ------------------------ 114 0.545 Yes 152 0.637 Yes 243 0.622 Yes 325 0.241 - 337 0.120 - 352 0.164 - 424 0.122 - 427 0.114 - 502 0.388 - 568 0.097 - 576 0.564 Yes 586 0.112 - 607 0.465 - 651 0.644 Yes 733 0.254 - 738 0.696 Yes 744 0.721 Yes 871 0.151 - 922 0.201 - 957 0.176 - 1020 0.672 Yes 1037 0.520 Yes 1189 0.158 - 1267 0.217 - 1321 0.316 - 1394 0.475 - 1555 0.447 - 1588 0.328 - 1612 0.185 - 1641 0.552 Yes 1759 0.334 - 1780 0.183 - 1842 0.674 Yes 1852 0.225 - 1939 0.090 - 1954 0.115 - 1976 0.591 Yes 2029 0.159 - 2093 0.281 - 2209 0.191 - 2262 0.211 - 2275 0.317 - 2382 0.632 Yes 2388 0.653 Yes 2451 0.659 Yes 2500 0.117 - 2508 0.186 - 2511 0.447 - 2721 0.194 - 2759 0.092 - 2775 0.059 - 2781 0.115 - 2808 0.066 - 2817 0.048 - 2822 0.052 -

Explain the output -- Processing locus 61: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACAAATTGTAACGCATATATATAAACCTCTGATAGAATAAAATGGTCACGTTGGTAATGTGCATTGTCCAAGAACATGA CTCTCATTGCCCTCTACATGATATAAATTATCTTCATTAACGCAAATGGAGAATCCTAATAAAGATCTAATGGAAACCAA TCCAAGAAAACTAAGATCATATTCCTACATATGTTTAATAGTATGTATATTTGTATTGGTTGTTTGTGCAATATTGTCCA GAGCAGAAGAAAAAGAAGGAAAAGGCGAAAACGATGATCATATACCAAAGATTTACTCTATTTTGGTTGAAGGTGAACCA TTGGCCTTTCGAGCTTCTACTAATATCAACAGCAAAGCTATGGCGTTAGAAGCAAAGAAGATAGAAGAGATTCATGACGA AATACTTGGAAGCACACTTGAAAAGGGAAGTTATACAAAGCTTTATAGCTTCAAACATGTCATCAATGCTATTGCAGTCC GTACCACTGCTTCTCAGGCCAAGAAACTCGGGAAAACAAAAGGAGTGAAGGCAGTAGAAGAAGACAAAGGAGTGAAACTA ATGACAACATACACTCCCGATTTCTTGGAACTTCCTCAACAAGTCTGGCAAAAGATCTCAAACGAAGGAGACAGACGTGC TGGAGAAGACATCGTGATCGGTTTCGTGGACACCGGAATTAACCCTACTCATCCTAGCTTCGCCGCACTTGATCTCACAA ACCCTTACTCATCAAATCTCTCACGTTTGCATTTCTCCGGTGACTGTGAGATCGGTCCCTTTTTTCCACCCGGATCTTGT AACGGAAAAATTATCTCCGCAAGGTTCTTTTCCGCCGGAGCTCGAGCTTCAGGCGCTCTTAACTCCTCCTTGGATATACT CTCTCCGTTCGATGCGTCCGGCCACGGAAGCCACGTGGCATCCATCGCAGCCGGGAACGCTGGAGTTCCGGTGATCGTCG ACGGATTCTTCTACGGCCGTGCTAGCGGTATGGCCCCACGTTCACGAATAGCCGTCTACAAGGCGATTTATCCATCAATC GGAACTCTTGTTGACGTAATTGCAGCCATCGACCAAGCAATTATGGACGGCGTAGATGTGTTGACGCTATCGGTTGGACC AGACGAACCACCGGTGGATAAGCCCACGGTACTCGGGATATTCGACCTCGCAATGCTATTGGCTAGAAAAGCCGGCGTGT TTGTGGTGCAAGCCGTAGGGAATAATGGCCCGTCGCCGTCTTCTGTGTTGTCTTATAGTCCGTGGGTCGTCGGTGTTGCT GCCGGAAATACTGACCGGTCTTATCCTGCCCCCCTCATCCTCGACGGTGGCCAAACCGTTCAGGGCGTTGGACTATCAGG TCCAACCCTTGGAGCTCCATTAGTTCAACACAGACTTGTCTTAGCCAAAGACGCTGTTAGAACAAACGGCTCGGTTCTAC AACCCTTAACCAGAGACATTGAAGAATGCCAGCGGCCGGAGAATTTTGACCCTGCCGCAGTTTTTGGAAGTATCGTGATA TGTACATTTTCTGATGGCTTCTACAATCAGATGTCGACCGTTCTTGCCATCACCCAAACCGCCAGGACTCTTGGTTTCAT GGGTTTTATACTCATTGCAAATCCTAGATTCGGCGATTATGTTGCAGAACCAGTGATATTTTCAGCTCCTGGTATTCTAA TCCCTACAGTATCAGCTGCCCAGATTATTTTGAGATACTACGAAGAGAAAACCTTTAGAGACACGAGAGGAGTGGCGACA CAATTTGGGGCACGTGCTAGAATAGGCGAAGGCCGAAACTCGGTCTTCGCCGGAAAAGCACCGGTGGTGAGCAGATTTTC TTCGAGAGGGCCAGCTTTTATTGATGCAACTCGGAGTCCTTTAGATGTACTTAAGCCAGATATTCTTGCACCTGGTCACC AAATTTGGGGTGCTTGGAGCCTCCCTAGTGCCTTTGATCCTATTTTGACAGGACGAAGCTTTGCCATATTGTCGGGAACA AGCATGGCGACTCCTCATATAGCAGGGATTGGTGCGCTCATAAAACAACTTAATCCTTCTTGGACTCCAGCCATGATTGC ATCTGCCATCTCCACCACGGCTAACGAATACGATAGTAATGGTGAAATTATATCAGCGGAGTATTATGAGCTAAGTAGAT TATTTCCATCTAATCATTTTGATCATGGTGCGGGCCATGTTAATCCTGCTAGAGCCCTAGATCCTGGACTAGTTTTACCC GCTGGTTTTGAAGACTACATAAGTTTCTTGTGCTCACTGCCAAACATTAGCCCAGCCACTATCCGAGACGCGACAGGAGT CTTGTGTACCACTACGCTTAGCCACCCAGCGAACCTTAACCATCCGTCAGTAACTATATCTGCACTTAAAGAGTCACTTG TGGTGAGAAGAAGTTTCCAAGACGTCTCCAACAAGACTGAGACATATCTTGGTTCGGTTTTACCTCCCAATGGTACAACT GTACGGTTAACCCCAACTTGGTTTACCGTACCACCACAGAAAACTCAAGACCTAGACATCGAATTCAATGTCACACAAGT CTTAAACAAGTTTACATTTGGTGAAGTTGTTCTTACTGGAAGCTTAAACCATATTATAAGAATACCGTTGTCAGTTAAAA CCATTTGAAAACATAAAATTTTATTGTTACTTGTCGATCTCACTTAAAATAAGGTCTTTATTCTTAAAAATTTCCAACGA AAAGGTTATTGACACTCCATCAAATAG ..........................................i..............N..................N... .................N...........................N.......................i.......... ..............................N...........N..................................... .................................N.............................................. .......................................i.................................N...... ........................................................N........N.............. ................................................................................ i............................................................................... ................................................................................ ................................................................................ ................................................................................ ...........N.................................................................... .............................i.................................................. ..........................................i............N........................ ....................................................i........................... ........................i....................................................... ................................................................................ ................................................................................ .........................i.....................................................i .............N................i...............................................N. ......................................i......................................... ................................................................................ ................................................................................ .......................N....................N................................... ................................................................................ ...i....................................................................N....... ......................................N..........................N.............. ........................N...........N........................................... ................................................................................ ................................................................................ .....................................................................N.......... ...................................................................N............ ................................................................................ ................................................................................ ........................... Pos Score Pred ------------------------ 43 0.544 Yes 58 0.418 - 77 0.304 - 98 0.163 - 126 0.238 - 150 0.544 Yes 191 0.148 - 203 0.101 - 274 0.350 - 360 0.641 Yes 394 0.218 - 457 0.411 - 466 0.147 - 561 0.652 Yes 892 0.198 - 990 0.758 Yes 1083 0.787 Yes 1096 0.366 - 1173 0.530 Yes 1225 0.667 Yes 1466 0.600 Yes 1520 0.522 Yes 1534 0.237 - 1551 0.702 Yes 1599 0.381 - 1639 0.509 Yes 1864 0.156 - 1885 0.401 - 2004 0.781 Yes 2073 0.407 - 2119 0.333 - 2146 0.226 - 2185 0.401 - 2197 0.334 - 2470 0.203 - 2548 0.259 -

Explain the output -- Processing locus 62: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGTTCTTATTCACTCTCGATTCTTCGCTTAGCTTCGTCTTCTTCATCGAAGATTTATCTCTGTAAGCTTCAAAACCTTTT TTTTTTTTTCGATTCGATTAATCTGAGATTATTTCCTCACTCGTTTTGATTCTGAATCAGACAATTCGCTGTGGATCTTC GGGGCGGGCGAATTTCATTGATTTTCCATTGAATCTGCGAGGTTACATATCCGGATTTGTGTTATCGGAGCTGAATTTAG GAATGGAGGTAGAGAAATCCAAGTATAGGAGCGAAGATTTGGATGTGGTGGAGGAGGAGGCTGATTTGAAGAAGAGTCGG CGAGATCGTGATCGGAGTAATGAGCGGAAGAAGGATAAGGGTTCAGAGAAGCGTCGAGAGAAGGATAGGCGGAAGAAACG AGTTAAGAGTAGTGATTCTGAAGATGATTACGATAGAGATGATGATGAAGAGAGGGAGAAGCGTAAGGAGAAGGAGAGGG AGCGGAGACGGAGGGATAAGGATAGGGTGAAGAGACGGTCTGAGAGAAGGAAGAGTAGTGATTCTGAAGATGACGTTGAA GAAGAGGATGAGAGGGATAAACGCAGAGTGAATGAGAAGGAGAGAGGGCATCGAGAACATGAGAGAGATAGAGGCAAAGA TAGGAAAAGGGATAGAGAGAGGGAAGAGAGGAAGGATAAAGAAAGAGAAAGGGAGAAGGATAGAGAGAGGAGAGAACGGG AACGGGAAGAGCGGGAGAAGGAGAGAGTTAAAGAAAGAGAAAGGCGGGAACGGGAAGATGGAGAGAGGGATAGGAGAGAG CGTGAGAAAGAAAGGGGTAGTAGGAGGAACCGGGAAAGGGAAAGGTCAAGAGAGGTGGGCAATGAAGAGAGTGATGATGA TGTCAAGCGTGACTTGAAACGTAGAAGAAAAGAGGGCGGTGAACGGAAGGAGAAAGAGCGTGAAAAGAGTGTTGGCAGAT CTAGCAGGCATGAAGATAGTCCAAAGAGAAAGAGTGTCGAGGATAATGGGGAAAAGAAAGAGAAGAAAACGCGAGAAGAA GAACTAGAGGATGAGCAGAAGAAGTTGGATGAGGAAGTGGAAAAACGAAGGAGAAGAGTCCAGGAGTGGCAAGAATTGAA GAGGAAGAAGGAGGAAGCTGAAAGTGAAAGTAAGGGTGATGCGGATGGTAATGAGCCCAAGGCCGGTAAGGCTTGGACTC TTGAAGGAGAATCTGATGATGAAGAAGGCCATCCGGAAGAAAAATCAGAAACAGAAATGGATGTTGATGAAGAGACTAAA CCTGAAAATGATGGAGATGCTAAGATGGTAGACTTGGAGAATGAGACAGCTGCTACTGTCTCTGAGAGTGGAGGTGATGG AGCTGTAGATGAAGAGGAAATTGATCCTTTAGATGCTTTTATGAATACTATGGTATTACCTGAGGTTGAGAAGTTTTGCA ATGGTGCTCCTCCCCCAGCAGTGAACGATGGTACTTTGGATTCTAAAATGAATGGGAAGGAAAGTGGTGACCGGCCAAAG AAAGGTTTTAATAAGGCCCTTGGTAGGATAATTCAAGGTGAAGATTCTGATTCTGATTATTCAGAACCAAAGAATGATGA TGATCCAAGTTTAGACGAAGACGATGAGGAGTTCATGAAGAGAGTAAAGAAGACAAAAGCTGAGAAGTTGTCCCTTGTTG ACCACTCAAAAATAGAGTATGAACCTTTCCGGAAGAACTTCTATATCGAAGTGAAGGATATCTCTAGGATGACACAAGAA GAAGTTAACACTTACAGAAAGGAATTGGAGCTGAAAGTTCATGGAAAGGATGTTCCAAGACCCATAAAGTTTTGGCACCA GACTGGGCTAACCAGCAAAATTTTGGATACTATGAAGAAGCTTAATTATGAAAAGCCAATGCCTATTCAAACACAAGCAC TCCCTATCATCATGAGCGGTCGAGACTGCATTGGGGTTGCAAAAACCGGTTCAGGTAAAACCCTTGGTTTTGTTTTGCCA ATGCTGAGGCATATTAAAGATCAGCCTCCCGTTGAAGCTGGTGATGGGCCAATTGGTCTTGTAATGGCACCTACTAGGGA GCTTGTTCAGCAGATTCACAGCGATATCAGAAAGTTTTCTAAGCCATTGGGTATAAGATGCGTCCCTGTGTATGGAGGAT CAGGAGTTGCCCAGCAAATTAGTGAACTTAAGCGAGGTACTGAGATTGTTGTGTGCACTCCTGGGAGAATGATTGATATC CTTTGCACAAGCAGTGGGAAAATCACTAATCTGCGAAGAGTCACATTTTTGGTAATGGATGAAGCTGATCGTATGTTTGA CATGGGTTTTGAGCCTCAAATTACTCGTATTATTCAGAACATTCGACCGGAACGCCAAACTGTGCTTTTTTCTGCCACTT TTCCACGCCAAGTTGAAACTTTGGCACGTAAAGTCTTGAACAAGCCTGTAGAAATACAGGTCGGTGGAAGGAGTGTTGTG AATAAGGATATAACTCAGTTAGTTGAAGTCAGACCGGAGAGTGATAGGTTCCTTAGACTTCTGGAACTTCTTGGGGAATG GTCTGAGAAAGGGAAAATTCTGGTCTTTGTTCAGTCGCAGGAAAAATGTGATGCCTTGTATAGGGATATGATTAAATCTA GTTACCCTTGTCTGTCTCTTCACGGGGGTAAGGATCAGACCGATCGTGAATCAACTATATCTGATTTTAAGAACGATGTC TGCAATTTGTTGATTGCCACAAGTGTTGCTGCTAGGGGTCTAGATGTGAAAGAGCTTGAGTTGGTTGTAAACTTTGATGC GCCGAACCACTATGAAGATTATGTGCATCGCGTTGGCAGGACAGGCAGGGCAGGGCGTAAGGGCTGTGCTGTGACATTTA TCTCCGAGGATGATGCAAAATATGCACCAGATTTAGTCAAGGCCCTGGAGCTTTCTGAGCAGCCAGTTCCTGATGATCTG AAAGCACTTGCTGATGGTTTCATGGTGAAAGTGAAACAGGGTATTGAGCAAGCTCATGGAACTGGCTATGGTGGTAGTGG CTTTAAATTCAACGAAGAGGAGGAAGAAGTTAGAAAAGCAGCAAAGAAAGCACAAGCAAAGGAGTATGGCTTTGAGGAAG ACAAGTCTGACTCAGAAGATGAGAATGATGTAGTAAGAAAGGCAGGTGGTGGTGAGATCTCACAGCAGCAGGCTACTTTT GCTCAGATAGCCGCCATTGCTGCTGCTGCCAAAGCTGCTGCTGCTGCTCCAGTGAGTGCTCCTGTGACAGCTAACCAATT ACTGGCAAATGGTGGCGGGCTTGCTGCCATGCCAGGTGTCCTTCCGGTTACTGTTCCTACCCTTCCTAGTGAAGGGGCAG GCCGTGCTGCAGCCATGGTTGCTGCCATGAATCTGCAACATAATCTGGCAAAGATTCAAGCTGATGCAATGCCTGAGCAC TATGAAGCAGAACTTGAAATCAATGACTTCCCACAAAATGCTCGTTGGAAGGTCACCCATAAAGAAACTTTGGGTCCAAT ATCAGAGTGGACTGGAGCTGCTATTACCACCAGGGGTCAGTTTTATCCTACAGGACGTATACCGGGACCTGGGGAACGGA AGCTGTACTTATTCATTGAAGGGCCTAGCGAAAAATCAGTTAAGCACGCAAAAGCTGAACTCAAGCGTGTTCTTGAAGAC ATTACGAATCAGGCCATGTCCTCACTTCCTGGAGGAGCATCCGGGAGATACTCAGTCCTTTAATGTAAGTCGAGAATTCT AGTCCAATTTACAATCTTTGGTGATAAATTTATGCTAAATGCTTTGCTTTAGCCTTGCATGCAGACTATTTTCTTTCTTA CA ................................................................................ ................................................................................ ................................................................................ ..i.......................................N..................................... ...................N............................................................ .......................N..............N..N..N................................... .....................................................................N.......... .......N.......................N..........................N..................... ................................................................................ .........................................................N...................... .............................................................i...........N..N..N ................................................................................ .........N...................................N.................................. ..........N.................N................................................... ......................................N.....N.....N............................. ...............N..N.....................................i...N.....N............. .......N..N.....N.......i...............N...................................N... ........N.......................N.......N........i.............................. N..........................N...................i...N............................ .........................................................................N..N..N .......................N..........N............................................. ..................N.................................................i........... ........................................N........N.............................. ...............................N...............N..........i..................... ...........i.................................................................... i..........................................N...................i................ .........................................................N.............N........ ....................................................................N........... ......................................................i...N.............N....... ................................................................................ .............................................................................i.. .............................................N....N................N............ ...........................................................................N.... ...........................................N................................N... ...........N........N........................................................... .........N..N........N..................................................N....... .............N.......i.................................N...........N............ .................................................................N.............. ..................N.....N..N.................................................... ................................................................................ ........N...................i................................................... ..............i...........i....................................N....i........... ................................................................................ ................................................................................ ...............N..............................................N................. ...............................N......N...................N..................... .. Pos Score Pred ------------------------ 243 0.808 Yes 283 0.325 - 340 0.424 - 424 0.380 - 439 0.204 - 442 0.198 - 445 0.241 - 550 0.463 - 568 0.238 - 592 0.281 - 619 0.310 - 778 0.471 - 862 0.664 Yes 874 0.181 - 877 0.206 - 880 0.224 - 970 0.112 - 1006 0.316 - 1051 0.311 - 1069 0.094 - 1159 0.406 - 1165 0.212 - 1171 0.092 - 1216 0.070 - 1219 0.125 - 1257 0.857 Yes 1261 0.275 - 1267 0.159 - 1288 0.210 - 1291 0.203 - 1297 0.137 - 1305 0.850 Yes 1321 0.097 - 1357 0.098 - 1369 0.078 - 1393 0.046 - 1401 0.352 - 1410 0.622 Yes 1441 0.266 - 1468 0.186 - 1488 0.785 Yes 1492 0.176 - 1594 0.381 - 1597 0.192 - 1600 0.108 - 1624 0.135 - 1635 0.472 - 1699 0.352 - 1749 0.565 Yes 1801 0.070 - 1810 0.234 - 1872 0.348 - 1888 0.185 - 1899 0.596 Yes 1932 0.541 Yes 2001 0.579 Yes 2044 0.185 - 2064 0.611 Yes 2138 0.357 - 2152 0.435 - 2229 0.408 - 2295 0.697 Yes 2299 0.120 - 2313 0.419 - 2322 0.571 Yes 2558 0.511 Yes 2606 0.233 - 2611 0.350 - 2628 0.311 - 2716 0.205 - 2764 0.454 - 2797 0.187 - 2812 0.375 - 2821 0.087 - 2890 0.170 - 2893 0.093 - 2902 0.122 - 2953 0.188 - 2974 0.455 - 2982 0.747 Yes 3016 0.443 - 3028 0.346 - 3106 0.391 - 3139 0.139 - 3145 0.187 - 3148 0.172 - 3289 0.296 - 3309 0.716 Yes 3375 0.891 Yes 3387 0.655 Yes 3424 0.269 - 3429 0.624 Yes 3442 0.285 - 3463 0.320 - 3478 0.244 - 3696 0.246 - 3743 0.217 - 3792 0.056 - 3799 0.042 - 3819 0.035 -

Explain the output -- Processing locus 63: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTTGCCCAAATCGCCTCCTCTCGAGAATTTCTCCGTCTCTCTCTCTCTCTCCAATGGGGTGGCTCAACACCATCTTCA ATTCTTCTTCTTCGACATTGATCAATGGCGGCTCTTCTCTATTTCCCTAAAATCTCTTCGCAAATGACTTCCTCACATTT CATCTCTTTCTCACCAATGGATTTGAGGAGACTTTCTCGCGCAAGCTACTTATTCACTCGCCGCTTGAAATTCATAGCGA AATCTCGAAAATGCTTTCATACAAGCCGATACCTGCAACAGTGTGTTCATCGGCCGGATAAATCGGAAGAGACTTCCTCT GATCGGCATTTACACGAACGATTATCTTCAGTTTTGTCGAAGAGATCTCTAGATTACGAGCAGTGTAAACAACTGATAAC TGTTCTATCGCCTCTCGAATTCGATAGGTTGTTTCCTGAGTTTCGATCCAAAGTTAATCCCAAGACGGCTTTGGATTTCT TCCGATTAGCTTCTGATTCGTTTAGTTTCTCCTTTAGTCTCCGTTCTTATTGTTTATTGATAGGTTTATTGCTTGATGCG AATCTGTTATCGGCTGCTAGGGTAGTTTTGATTCGTTTGATCAATGGGAATGTACCGGTTTTGCCTTGTGGTTTGAGAGA TAGTCGAGTTGCGATTGCGGATGCAATGGCGAGTTTGAGTTTGTGTTTTGATGAAGAAATCAGAAGGAAAATGTCGGATT TGTTGATTGAAGTTTATTGCACACAGTTCAAGCGTGATGGTTGTTATTTAGCTCTTGATGTATTTCCTGTTCTTGCAAAC AAGGGTATGTTTCCTTCTAAGACGACTTGCAACATTTTGTTGACTTCTCTAGTGAGAGCTAATGAGTTTCAGAAGTGTTG TGAAGCTTTTGATGTTGTTTGCAAAGGGGTTTCTCCGGATGTTTACTTGTTCACTACAGCGATTAATGCGTTTTGCAAGG GAGGTAAAGTTGAGGAGGCCGTTAAGTTGTTCTCAAAAATGGAAGAAGCGGGTGTTGCACCAAACGTTGTCACTTTTAAT ACTGTTATTGATGGACTTGGGATGTGTGGAAGGTATGATGAGGCGTTTATGTTTAAAGAAAAGATGGTAGAAAGAGGCAT GGAACCAACTCTTATCACTTATAGTATTCTTGTCAAGGGTTTGACCAGGGCTAAGAGGATTGGTGATGCTTATTTTGTCT TGAAGGAAATGACAAAGAAAGGGTTTCCTCCTAATGTTATTGTATATAATAATTTGATTGATAGTTTCATCGAGGCTGGA AGTTTGAACAAAGCTATTGAGATAAAGGATCTCATGGTTTCGAAAGGGTTGTCTCTCACATCGTCGACGTATAATACTCT CATAAAGGGATACTGCAAGAACGGTCAAGCAGATAATGCGGAGCGTCTCCTGAAGGAAATGCTGTCGATTGGTTTTAATG TAAATCAGGGCTCCTTTACATCTGTGATTTGCTTGTTGTGCAGTCATCTTATGTTTGACTCAGCACTTCGTTTTGTTGGA GAGATGCTATTACGCAACATGAGTCCTGGTGGTGGATTACTTACTACATTGATTTCTGGTCTCTGTAAGCATGGGAAACA CTCAAAGGCACTAGAACTTTGGTTTCAATTTCTAAATAAAGGTTTTGTAGTGGACACGAGGACTTCAAATGCTTTACTTC ATGGACTCTGTGAAGCAGGGAAGCTGGATGAAGCTTTTAGGATTCAAAAGGAGATATTAGGGCGTGGCTGTGTTATGGAT AGAGTTTCCTATAACACGTTGATTTCTGGATGTTGCGGAAAGAAGAAACTAGATGAAGCTTTTATGTTCTTGGATGAAAT GGTTAAGAGAGGACTTAAGCCTGACAATTATACTTATAGCATATTGATTTGCGGGCTGTTTAATATGAATAAAGTTGAGG AGGCCATACAGTTTTGGGATGATTGTAAACGGAATGGCATGCTTCCAGATGTTTATACATATTCGGTGATGATAGATGGA TGTTGTAAAGCTGAAAGAACAGAAGAGGGTCAGGAATTTTTTGATGAGATGATGAGCAAAAACGTGCAGCCAAATACTGT TGTTTACAATCATCTTATCAGAGCATACTGTAGAAGCGGAAGGCTATCGATGGCCTTGGAACTCCGTGAAGATATGAAAC ACAAAGGAATTTCACCTAATTCTGCTACATACACTTCACTAATAAAGGGGATGTCAATCATTAGCCGTGTTGAGGAGGCA AAACTCCTCTTCGAGGAGATGAGGATGGAGGGTTTGGAACCGAATGTCTTTCATTATACAGCACTTATTGATGGATATGG TAAATTGGGTCAGATGGTCAAAGTTGAATGTCTTCTGCGTGAGATGCATTCAAAGAACGTACACCCAAATAAGATTACCT ACACTGTGATGATTGGTGGGTATGCTAGAGATGGGAATGTAACGGAAGCTTCTAGGCTTCTAAATGAGATGAGGGAAAAA GGAATTGTTCCGGATAGTATCACATACAAAGAATTTATATACGGATATCTTAAGCAGGGAGGTGTGTTGGAAGCTTTTAA AGGTTCTGATGAAGAAAATTATGCAGCAATCATTGAAGGATGGAACAAACTCATTCAGTAACTGATGAATAAGAAGAAGA ACAGGTTTCTATTCTGGTTCTTATTTTTCCTATTAGTTGCTCTGATAGCTTCAGTTTATTAATGTATAATGTTCTGATTT .......................................................i........................ ........................i......................................N................ ................N............................................................... ..........N..................................................................... ................................................................................ ................................................................................ ...........................................................................N.... ...........................................N.....N.............................. ....................N....i........................N...................i......... ....................................N....................N...................... ......i......................................................N.................. ...........N..........................N..........................N.............. ......................................i......................................... ..........N..........i............N..N..........N..............i..............i. .................................................................N.............. ........i........................N.............................................. .................................i.............................................. ...................................N......................N..................N.. ..................................................N............................. ...N..............N...................................................N......... ....................................................................N........... N..........................N..............................................i..... .............................N......................N..........N.........N....i. ................................................................N............... ..................N..............N....i.........N...................N......N...N ...........................................N....N..N............................ .................................................N.......................i...... ..................................................N............................. ..................N.....i..................N..........................N.....N... .............i.............i...............N.................................... ........N............N........N.....N..........................N....N........... ................................................................................ ........N...........N..................N........................N............... .............................................................N......N........... Pos Score Pred ------------------------ 56 0.564 Yes 105 0.740 Yes 144 0.468 - 177 0.354 - 251 0.392 - 556 0.170 - 604 0.278 - 610 0.380 - 661 0.250 - 666 0.715 Yes 691 0.067 - 711 0.772 Yes 757 0.340 - 778 0.061 - 807 0.532 Yes 862 0.306 - 892 0.159 - 919 0.078 - 946 0.102 - 999 0.787 Yes 1051 0.074 - 1062 0.626 Yes 1075 0.059 - 1078 0.145 - 1089 0.424 - 1104 0.728 Yes 1119 0.733 Yes 1186 0.089 - 1209 0.711 Yes 1234 0.078 - 1314 0.646 Yes 1396 0.207 - 1419 0.389 - 1438 0.322 - 1491 0.323 - 1524 0.153 - 1539 0.451 - 1591 0.491 - 1669 0.344 - 1681 0.129 - 1708 0.180 - 1755 0.560 Yes 1790 0.167 - 1813 0.171 - 1824 0.460 - 1834 0.121 - 1839 0.537 Yes 1905 0.329 - 1939 0.186 - 1954 0.270 - 1959 0.580 Yes 1969 0.112 - 1989 0.371 - 1996 0.092 - 2000 0.191 - 2044 0.266 - 2049 0.310 - 2052 0.421 - 2130 0.484 - 2154 0.581 Yes 2211 0.335 - 2259 0.381 - 2265 0.662 Yes 2284 0.136 - 2311 0.156 - 2317 0.155 - 2334 0.569 Yes 2348 0.580 Yes 2364 0.194 - 2409 0.497 - 2422 0.176 - 2431 0.338 - 2437 0.253 - 2464 0.126 - 2469 0.395 - 2569 0.127 - 2581 0.091 - 2600 0.304 - 2625 0.213 - 2702 0.047 - 2709 0.045 -

Explain the output -- Processing locus 64: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTATTATGAAAATTATGAAATATCGATAAGGGAAAAACTGTATGGAAGTGTAAAAGACACCAAAACAGATATTGAACCA AAGGTGAAGCAAAGAAGTTGATTCAAAGTTGCCACTTTATTTAACCTTTTGCACCAGTATATATACACACACATGGATAC TAACCCAATCATTCATCACAACACATCAGATCCTTCACCATATATTTCTCTCAACCTCGCCAATATGGCCCAACGCTTTC AATTGAACTCACAAACCCTCCCAACGGCCGACCCTCTCAAAAGGGTCTCCCTTATTCCAAGATCCGCCGAGCAGAGGCTG GCTGATAACGCAGGCGAGAGGCGTCCTTTAGCACCGAGATCTCACGAAGACAACCCCTTTGGGGGTCATACTGATGATCA CCATGTTGCTGCTCCTGCGGACCATAATAAGGTGATGGATCATAATAGCGAGAATCTCGGTTCCATTGTCCCTAAGACTG CACATTACCCTCATCCATCTGAGGAAATCCTTGATGCTAATATCCGACATTCGATGGTTCCGAAATCATTGGGTCCCAAT TCGCTCGGTGGAAGATTTGGTCCGGGAAAGAAACAAGCTTTCCATAGAAATGGCAGACCCATGTTTTCACTTTCTGATGA CCGAGTCATGGCTGATAGAGTCTTGAAGACTCACAGTCCAGACATGATCTTCTTTGATGTCACATCACTTCTCTCCGTTG TTAATGACATCTTCAAGTCTCATGTCCCATCCATTGACTCCTCTGCTCCTAAGCCATCACTCGTCTTCAAGGACTACGCT GATCATACCTCCTTTGAAACTTTTGCGGATCTCATTGATCAAATCTCTTGCGAGATCGACTGCAAGTGTCTCCATGGCGG TGAGTCTCACGGTATGATGACATCAGGGCTTCATTTGGATAGTCGCAACACGACGACGTTCTCTGTTCTGTCTTTGGTCT CCAAATACCGTTGGGATGCTAAGCTAGTCCTTGTCCTATCTGCTTTGGCCGTGAAATACGGCGTTTTTCTCCTCCTCGCG GAGACACACGCAACAAATCAGCTCACAAAATCTCTGGCTCTCATCAAACAGCTCCCTAGCATCTTCTCCCGGCAGAACGC TCTACACCAGCGTCTCGACAAGACCCGAATTCTGATGCAAGACATGGTTGATCTCACCACTACCATCATCGATATCTATC AACTCCCGCCAAACCACATCACAGCCGCGTTTACCGACCATATCCCCACTGCGGTTTACTGGATTGTTCGCTGTGTTTTG ATATGTGTTTCTCACATCAGTGGCGCCAGTGGTTTCAAGCAGGATCAAATCATGTCGTTTATGGAAGTTTCGGAGATACA TGAGAATAGCGAAAGGCTTAGGAAGATCAATGCTTACTTGTTAGAACAATTCAAGAAGAGTAAAATGACCATCGAGGAAG GTATAATCGAAGAAGAGTATCAAGAACTCATCCAGACGTTCACTACTATCATTCACGTCGACGTTGTTCCTCCTCTACTT CGTCTTCTCAGACCCATTGATTTTCTTTACCACGGAGCTGGTGTTTCAAAGAGACGCGTCGGGATCAACGTGCTCACCCA AAAGCACGTTCTGCTTCTGATATCAGACCTAGAAAACATCGAAAAAGAGCTCTACATCCTGGAATCGCTCTACACAGAAG CATGGCAGCAGTCATTCGAGATTCTTTGGGTTCCAGTTCAAGATTTCTGGACAGAAGCTGACGATGCCAAGTTCGAGGCC CTTCACATGAACATGAGGTGGTACGTTCTTGGAGAGCCTCGGAAATTAAGACGAGCCGCGATAAGGTTTGTGAGGGAATG GTGGGGTTTCAAGAACAGGCCTATACTCGTTGCACTTGATCCCAAAGGTCAAGTTATGTCAACCAACGCCTTTCCTATGG TCTGGATCTGGCAACCCTTTGCTCACCCTTTTACCACCGCAAGGGAACGTGATCTATGGAGCGAACAGGAGTGGAATCTT GAGTTCTTGATTGATGGCACTGATCCTCATTCCTTAAACCAGCTTGTTGATGGAAAATACATATGTCTCTACGGAGGAGA AGATATGCAATGGATCAAAAACTTCACGAGTCTCTGGCGTAATGTAGCGAAGGCCGCAAACATTCAACTTGAGATGGTTT ATGTCGGGAAAAGAAACCCTAAGAATGGAATCCAGCCGATAATCAACACAATCCGGGAGGAAAATCTCAGCCATACTTTG CCGGACTTGTTCCAGATCTGGTTTTTCTGGACCAGGGTCGAGAGCATGTGGGAATCGAAGCAGAGGATGCTGAAAGCCCA CGGAATCAAGGGGAGAGAAGGGTTTAAAGAAGAGGAAAAGGACCTTGTTCTGCAAGAAGTGGTTGCTATGTTAGGTTATG GAGGAGAAGGAGATGGATGGGGACTGGTGAGTAAAGCATCGGACATGATGGTTCGAGCCAAAGGGAACTTGTTTTCTAGG GGTTTGGCTGAATTTAACGAATGGGAAGTCAACATCCCCACAAAGGGTTTTCTTACAGCGCTCAATGATCATCTCTTAAT GCGTCTCCCACCACATCACTGCACAAGGTTCATGCTTCCTGAGACTGCTGGAATTATACCTAATGAGGTTGAGTGCACCG AGTGCCGTAGGACTATGGAAAAGTATTACTTGTACCAATGCTGCCTTGAGTAAGATCCCATAATACTGGGGAACAAGTTG ATTTGGAAGCCAACAACTTGTTCCTATAAAACCAAATAAGTTTGCGTCTTTTTTTTCCTCCTTGTTGTGAGT ......N........N..........................N..................................... ........................................................................i....... ................................................................i............... ................................................................................ .........................................................................N...... ..N...............................i............................................. .................................N...................N.......................... .................................................N..........N...............N... .......i...................................N............N....................... ...N.................N.......................................................... .........................................................................N...... .............N..i............................................................... ...............N................................................................ ................................................................................ ..................................i........i.................................... ................................................................................ ..i................................................i........i..................N .............................N..................................i............... ................................................................................ ................................................................................ ................................................................................ ......N.....i................................................................i.. .......................................................i....................i... .......................................................i........................ .............N...................................N............N................. ....i....i...............................N...............................i...... N.......................i....................................................... .............................................i....................i............. ...................................................................N.........N.. ............i...N...........................N..i................................ ....................N...........................................N.............N. ...............................N..............................N................. ..............N......................N.......................................... ........................................................................ Pos Score Pred ------------------------ 7 0.174 - 16 0.276 - 43 0.164 - 153 0.582 Yes 225 0.734 Yes 394 0.141 - 403 0.088 - 435 0.680 Yes 514 0.247 - 534 0.292 - 610 0.345 - 621 0.328 - 637 0.108 - 648 0.734 Yes 684 0.440 - 697 0.383 - 724 0.205 - 742 0.360 - 874 0.256 - 894 0.347 - 897 0.697 Yes 976 0.116 - 1155 0.787 Yes 1164 0.724 Yes 1283 0.642 Yes 1332 0.722 Yes 1341 0.685 Yes 1360 0.156 - 1390 0.224 - 1425 0.511 Yes 1682 0.352 - 1744 0.537 Yes 1767 0.453 - 1773 0.672 Yes 1838 0.539 Yes 1896 0.521 Yes 1917 0.577 Yes 1976 0.596 Yes 2014 0.420 - 2050 0.140 - 2063 0.242 - 2085 0.833 Yes 2090 0.547 Yes 2122 0.336 - 2154 0.553 Yes 2161 0.236 - 2185 0.607 Yes 2286 0.684 Yes 2307 0.703 Yes 2388 0.279 - 2398 0.232 - 2413 0.672 Yes 2417 0.494 - 2445 0.292 - 2448 0.583 Yes 2501 0.449 - 2545 0.239 - 2559 0.345 - 2592 0.361 - 2623 0.319 - 2655 0.227 - 2678 0.279 -

Explain the output -- Processing locus 65: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGGCAAAAATTGTATACTTAAATATTCTTCTGGTTGGGAATGTAGTCTGCGAAACATTATATCTTAATCCTGAAATGTGT GCTTGGAAGTGTAAAATGTAAACCACTCTTAATAAGCTAAAGACTTCACTCTCTCTCTCTCTCTCTTCATTATCCCCTAC ACTTCTCTTTCTAAAACCTCTGGGGTTCTTGTTAAAGATGATGTCTTTGCTCCCAAATCCCGACCCGATTACTGTACCCC TGGTCCTCAAGCTTTGCTCCTTTCCTCCGCCACGGAGGCTCTTCTCTCTCAGGCTCAGGCGCTTCACCCGAAAGTCATCA TCTCTTCTTCCGTTGGTCGCTGTTTCGTCTCTCTCCGCTACTGCCGCAAAACCTACCAGATGGAGAGAGAAGCCGGAATT GGCGGAAAGCGACTCAATTTCCCTCCTCAACGAGAGGATTCGGCGTGACCTCGGCAAGAGAGAGACTGCTAGACCGGCCA TGGACTCTGAGGAGGCCGAGAAGTACATTCACATGGTCAAGGAACAACAAGAGAGGGGTCTGCAGAAGCTCAAAGGAATT AGGCAAGGTACAAAGGCTGCTGGTGACGGTGCTTTTAGTTACAAGGTTGACCCTTACAGTCTCCTTTCCGGTGATTATGT GGTGCACAAGAAAGTAGGCATTGGGCGTTTTGTTGGGATCAAGTTTGATGTCCCCAAGGACTCCTCTGAGCCCCTCGAAT ATGTCTTTATAGAGTATGCTGATGGTATGGCCAAGCTTCCCCTCAAACAGGCCTCGCGTTTGCTCTACCGATACAATCTT CCAAATGAGACTAAACGGCCTCGGACTTTGAGTCGGCTGAGTGACACTAGTGTTTGGGAAAGAAGAAAGACCAAAGGAAA AGTAGCAATTCAGAAAATGGTCGTTGACTTGATGGAGCTATATCTTCATAGGCTTAGACAGAAGAGATATCCATATCCGA AGAACCCCATCATGGCTGATTTTGCGGCTCAATTTCCTTATAACGCCACTCCTGACCAGAAGCAGGCTTTCCTGGATGTT GAAAAGGATTTGACTGAGAGAGAAACACCTATGGACCGATTGATCTGTGGAGATGTTGGCTTTGGTAAAACAGAGGTTGC TCTACGAGCCATCTTTTGTGTGGTCTCAACTGGAAAACAAGCTATGGTTTTAGCACCGACAATTGTATTGGCGAAGCAAC ATTACGATGTAATCTCAGAGCGGTTTTCCTTGTATCCACACATCAAAGTGGGCCTTTTAAGTCGGTTTCAGACCAAAGCT GAAAAGGAGGAGTATTTGGAAATGATAAAAACTGGACATCTCAATATCATTGTGGGTACTCACTCACTTCTCGGGAGCCG TGTTGTGTACAGTAATTTAGGCCTCCTTGTGGTTGATGAGGAGCAGAGATTTGGGGTCAAGCAGAAAGAAAAGATTGCAT CTTTCAAAACATCCGTGGATGTGCTTACATTGTCCGCAACACCCATACCAAGGACACTGTACTTAGCTTTGACTGGATTC CGGGATGCTAGTTTAATCTCCACACCGCCTCCGGAGAGGATTCCAATAAAAACCCATCTTTCGTCATTCCGTAAGGAAAA GGTGATTGAAGCAATAAAAAATGAGCTGGATCGTGGTGGCCAAGTTTTTTATGTCTTGCCTCGGATTAAAGGACTAGAAG AAGTGATGGATTTTCTTGAAGAAGCATTTCCAGATATTGACATTGCTATGGCACATGGAAAGCAATACTCGAAACAACTA GAGGAAACCATGGAGAGATTTGCGCAAGGAAAGATCAAAATCCTCATATGTACTAATATTGTTGAAAGCGGACTTGATAT TCAAAATGCAAATACCATAATCATTCAGGATGTTCAACAATTTGGGCTCGCTCAGTTGTACCAGTTGCGTGGAAGGGTTG GTCGGGCAGATAAAGAAGCTCATGCCTACCTATTTTATCCCGATAAATCGCTGCTCTCTGATCAAGCACTGGAAAGGCTT AGCGCTCTTGAAGAGTGCCGTGAACTTGGACAAGGCTTCCAACTTGCAGAGAAGGATATGGGTATAAGAGGTTTTGGGAC AATCTTTGGAGAACAACAGACGGGGGATGTTGGAAATGTCGGTATCGATCTCTTCTTTGAAATGCTTTTTGAGAGTCTCT CCAAGGTGGAGGAACTCCGTATTTTTTCGGTTCCATATGACCTCGTGAAGATTGACATAAATATAAATCCCCGGCTACCC TCGGAGTATGTAAATTACCTGGAAAATCCGATGGAGATCATTCATGAAGCTGAAAAAGCAGCAGAGAAAGATATGTGGAG TCTAATGCAATTTACAGAGAACCTGCGTCGCCAATATGGGAAAGAGCCTTACTCCATGGAAATCATTTTAAAGAAGCTGT ATGTGAGACGCATGGCGGCTGATCTTGGAGTAAACAGAATTTATGCATCAGGCAAGATGGTTGTCATGAAAACAAATATG AGTAAGAAGGTGTTCAAGCTGATCACAGATTCCATGACTTGTGACGTTTACCGAAGCTCCTTGATATATGAAGGAGATCA AATAATGGCGGAACTTCTGTTGGAGCTACCAAGAGAACAGTTACTGAACTGGATGTTCCAGTGCTTGTCAGAACTTCATG CATCACTTCCTGCTCTTATCAAATATTAGTTCCCCCTTGTCAGTTTCTCTTGCGTGTTAAAGGAAGAGAGACGCCCTCAG CAATTGTGTTGCTGTGAAGCTTTCTTCACAGAATAGAAGAGAAGAGAG .......................................N..................................N..... ...............i................................................................ .....................................N..i....................................... ................................................................................ ...........................................................i.................... ...............................................................................i ................................i............................................... ............................................................................N... ...............................................N................................ i..............N.....N....i..................................................... ....N........................................................................... ................i..............i................................................ ...........i...............................................................N.... ..............................N.....................N........................... ...........................................i.................................... ......N......................................................................... .....................i.......................................................... ...................................N............................................ ..................N............................................................. ....N........................................................................... ....................N.............................N............................. .....i.........................................N......N......................... .........i.....................................N................................ .....N.......................N.................................................. .....................N.......................................................... .........................................................i...................... ..........................N........N.........................i.................. ....................................N........................................... .......N......................i............N............................N....... ....N..............................N...................N........................ N..........N..............................N.............i........N...........N.. .................................N.................................N............ ....N...............................................N........................N.. ................................................................................ ................................................ Pos Score Pred ------------------------ 40 0.323 - 75 0.261 - 96 0.545 Yes 198 0.328 - 201 0.631 Yes 380 0.863 Yes 480 0.826 Yes 513 0.855 Yes 637 0.142 - 688 0.295 - 721 0.597 Yes 736 0.235 - 742 0.357 - 747 0.755 Yes 805 0.272 - 897 0.657 Yes 912 0.587 Yes 972 0.775 Yes 1036 0.081 - 1071 0.486 - 1093 0.210 - 1164 0.717 Yes 1207 0.330 - 1302 0.735 Yes 1396 0.181 - 1459 0.145 - 1525 0.260 - 1621 0.367 - 1651 0.163 - 1686 0.678 Yes 1728 0.434 - 1735 0.150 - 1770 0.501 Yes 1808 0.279 - 1846 0.303 - 1870 0.398 - 1942 0.446 - 2058 0.798 Yes 2107 0.205 - 2116 0.318 - 2142 0.544 Yes 2197 0.172 - 2248 0.099 - 2271 0.504 Yes 2284 0.144 - 2313 0.457 - 2325 0.406 - 2356 0.266 - 2376 0.479 - 2401 0.288 - 2412 0.486 - 2443 0.200 - 2457 0.686 Yes 2466 0.448 - 2478 0.288 - 2514 0.214 - 2548 0.197 - 2565 0.240 - 2613 0.095 - 2638 0.335 -

Explain the output -- Processing locus 66: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGCGTCAGTTAACAACTTATCGCTCTAGCTAACAGCTAAAGTTTTGTTACCAATCAGACTCTTAAATCTTAAGAAATGGA GTAATCCATCTCAGTGCATAAGTGTCACAAAATGATCGAGACTCGTTGGTTACAAACTCTTTATCACATTTGAAGAAGCT TAACTTAAGTTGTTTTGATTTTCGAGAAACCGATAGAGTAACAAAGAAGTTAACATCCCAATTAATTATGTTCCTAAGCT TTATTTTCTTAGTGTCGGATTGGTTAGATTTTCTCATAGACGCTATTCCGCTAAAAAACTATGACTCTACACAATTACAC AAGATGTGGGTTAGTAAAAATTTGAAGTCTGAAAAAAAAACAGAAAATTCAGATTCAAGAGCCATCTTCTTGTCTTATCT ACACGTGTCACATACTGAACTCCTCCAACAAGAGAAAGATGTGTCCGAAGCTCCCTCGCTCGAATGGCGAATCCTCTACA CCTTGAGTTCGTTTAGCCAATCTCAACAAACGCTAAAGAACGACGTCGTTTTGTTGATCGTAATTCTAAAAAATCAATCA GCAATGCTGATGAAAAGTCTTCACTTGTTGTCTTCCTCAATCCTCCTCCACCGCTTTGCTTCTCGCAGCATTGCATCTTT GTCTACTCTACCCAGAAGAAGAAATATCGTCCGTATACATAACCCTTCGTTTTCTAGCCCTTTCCCGCCGATTCTCTCTA GACCCATTTCCTCAGGCAAGGTCTTAGCCATGGCGGAAGAGTCTTCTTCTTCTTCGACCTCGTCAACATCTCAGAAACAT ACCAACCGTTTAGCCGCTGAACACAGTCCTTATCTTCTTCAACACGCGCATAATCCGGTTGATTGGTACCCATGGGGAGA AGAAGCTTTTGAAGAAGCAAGGAAGAGAGACGTGCCCATCTTCTTATCAATTGGATACAGTACCTGTCACTGGTGCCATG TCATGGAAGTTGAGTCATTTGAAGATGAAGAAGTGGCAAAACTCTTGAACAATTCATTCGTTAGTATCAAGGTTGATCGA GAAGAGCGGCCTGATGTCGACAAGGTTTATATGTCGTTCGTCCAGGCTCTGTATGGTGGAGGAGGGTGGCCACTGTCGGT ATTCCTTTCACCTGATTTAAAGCCGTTGATGGGTGGAACCTACTTTCCTCCAAATGACAATTATGGACGACCAGGATTCA AAACTCTCCTTAAGAAGGTGAAAGATGCGTGGAACAGTAAGAGGGATACTCTAGTGAAGAGTGGTACATATGCTATCGAA GAACTCTCTAAAGCTTTATCTGCAAGTACTGGAGCCGATAAATTGTCAGATGGGATTTCTCGGGAGGCTGTGAGTACATG TGCAAAACAACTTTCTCGGAGCTATGATTCGGAATTCGGTGGGTTTGGATCTGCACCAAAGTTCCCGAGGCCTGTCGAGA TCCAGCTGATGCTTTATCATTACAAAAAGTTGAAGGAATCGGGGAAAACTAGTGAGGCTGATGAAGAGAAAAGTATGGTT TTATTCAGCTTGCAAGGCATGGCAAATGGAGGGATGCATGATCACATTGGAGGCGGGTTTCATAGATACAGTGTCGATGA ATGTTGGCATGTTCCACATTTTGAGAAAATGCTGTATGATCAAGGGCAACTAGCTAATGTGTACCTCGACGGTTTTAGCA TTACCAAGGACGTTATGTATTCTTATGTGGCAAGAGACATCCTTGATTATCTTAGAAGAGACATGATTGCACCTGAAGGT GGAATATTCTCAGCTGAAGATGCCGATAGCTTTGAGTTCGAGGGGGCAAAGAGAAAGAAAGAAGGAGCTTTCTATATATG GACAAGCGATGAGATTGATGAAGTCCTCGGGGAGAATGCTGATCTTTTCAAGGAGCATTATTATGTTAAGAAATCAGGTA ACTGCGATCTTTCGAGCAGGAGTGACCCGCACAATGAATTTGCTGGGAAGAACGTGCTGATCGAAAGAAATGAGACGTCT GCTATGGCATCAAAGTTTAGCCTCTCTGTTGAGAAGTATCAGGAGATATTAGGTGAATGCCGAAGAAAGCTTTTCGATGT CAGATTGAAGCGACCTAAGCCGCATCTAGATGATAAGATAATCGTGTCTTGGAACGGTCTTGTTATCTCGTCTTTCGCAA GAGCTTCCAAGATTCTTAAGGCTGAGCCTGAAAGCACCAAATATTATTTCCCTGTGGTGAATAGTCAGCCAGAGGATTAC ATAGAGGTTGCAGAGAAAGCAGCTTTGTTTATCAGAGGAAATCTCTATGATGAGCAGTCTCGCAGATTACAACACAGTTA CAGACAGGGGCCATCCAAAGCTCCTGCCTTCTTAGACGATTATGCTTTCTTGATCTCTGGTTTACTCGATCTTTACGAGA ATGGAGGTGGGATCGAATGGCTTAAGTGGGCGATTAAACTTCAAGAAACACAGGATGAGTTATATCTGGACCGAGAAGGA GGAGCATACTTCAATACTGAAGGACAAGACCCATCGGTTCTTCTTCGTGTAAAAGAAGATCATGATGGTGCAGAACCTTC AGGGAACTCTGTTTCTGCTATCAATCTTGTGAGGTTAGCTTCCATAGTCGCCGGAGAAAAAGCTGAATCCTACCTCAACA CGGCACATCGTCTCCTGGCAGTCTTTGAGTTGAGGTTAAGAGAACTGGCGGTGGCTGTGCCATTAATGTGCTGCTCCGCA GATATGATCTCTGTTCCGTCGAGGAAACAAGTTGTATTGGTTGGTTCTAAGTCTTCCCCAGAGCTTACCAACATGCTTTC TGCAGCACATTCAGTCTATGATCCTAATAAAACGGTGATTCACATAGATCCCTCTAGTTCTGATGAAATCGAGTTTTGGG AAGAGCATAACAGTAATGTAGCTGAGATGGCTAAGAAGAACAGAAACTCAGAGAAAGTGGTGGCTCTTGTTTGTCAACAC TTCACTTGCAGTCCTCCTGTCTTTGACTCTTCTTCTCTCACTCGCTTGCTTTCCAAGTAAACAAAGATCATTCCATGTTT AATAGTTGCTTGTACTGTAAGGAACAGATCAACGTTGTTCTCTCTGTTACAGTCTTACAGAGTAGATAAATAAAGAAGA ...........................................................................i.... ...............................N................................................ ...................................................................N............ ............................................................N................... ...N............................................................................ ......................................i........................i................ ................................................................................ ...N.....N...................................................................... ................................................................................ .............................i.................................................. .......................................................................N........ .............................................................................i.. ..i.....................N....................................................... .............N................N.....................N........................... ............................N........................N........N................. ........................N............................................N.......... .................................................i...........................N.. .......................N........................................................ ........N...................................................N.............i..... ..................i......N.......N...N......................................N... N.......N...................i......N....................N....................... ..............i.........N.....................................i................. ...................N.........................................................N.. ........N........N.................N..........................N................. .................................N...................................N.......... ...N....................................................N...................N... .............................N.................................................. ................................................................................ ..............................................N..N.............................. .........................................N...................................... i...............N.....................................i......................... .............................................................N..i............... ................................................................................ .................................................................N.............. ...N....................................................................i....... .................N............................................N................. ...............N..........i..................................................... ..........................................................................N..... ............................................................................... Pos Score Pred ------------------------ 76 0.588 Yes 112 0.497 - 228 0.262 - 301 0.331 - 324 0.378 - 439 0.507 Yes 464 0.839 Yes 564 0.433 - 570 0.414 - 750 0.817 Yes 872 0.300 - 958 0.644 Yes 963 0.861 Yes 985 0.271 - 1054 0.445 - 1071 0.453 - 1093 0.276 - 1149 0.469 - 1174 0.195 - 1183 0.490 - 1225 0.438 - 1270 0.236 - 1330 0.534 Yes 1358 0.373 - 1384 0.219 - 1449 0.149 - 1501 0.302 - 1515 0.675 Yes 1539 0.562 Yes 1546 0.225 - 1554 0.441 - 1558 0.158 - 1597 0.097 - 1601 0.219 - 1609 0.136 - 1629 0.796 Yes 1636 0.255 - 1657 0.279 - 1695 0.623 Yes 1705 0.189 - 1743 0.862 Yes 1780 0.378 - 1838 0.494 - 1849 0.307 - 1858 0.280 - 1876 0.346 - 1903 0.143 - 1954 0.334 - 1990 0.177 - 2004 0.474 - 2057 0.320 - 2077 0.318 - 2110 0.074 - 2287 0.151 - 2290 0.330 - 2362 0.218 - 2401 0.676 Yes 2417 0.439 - 2455 0.538 Yes 2542 0.301 - 2545 0.556 Yes 2706 0.491 - 2724 0.228 - 2793 0.708 Yes 2818 0.103 - 2863 0.139 - 2896 0.165 - 2907 0.608 Yes 3035 0.122 -

Explain the output -- Processing locus 67: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTACGAAAGGGAAAAAAATTATTGAACAACGACCAGCAAACGAGGCAATAAGCGCCATTCAAAGACTATATAGCGTGG GCATTTTCGTAATTTCACTAATCGCTTAGCCGTTGGACACCAATGCCATTCAAGAAATTTCGTCGGAGTCTAAACTTCAT TATTTGCTTACCAAAACTAAAAGAGTGCGCTCGTGGACAAATTTTAGGCTATAAAATGGAGACGAAGGCGATTGGATTTC ACCACCTTTCTCCACCACAAAATATTGTGTTATGGCTCCTGATCAATCTTATCAATATCCATCTCCTTCGTATGAATCGA TACAGACCTTTTACGATACCTGATGAGGATTGGCCGGGTCCTAGATGCGGCCATACTCTCACGGCGGTTTTCGTCAACAA TTCCCATCAGCTTATCCTCTTCGGCGGCAGTACTACTGCCGTCGCCAACCATAACTCCTCCTTACCCGAGATCAGTTTAG ATGGCGTCACCAATTCGGTCCATTCTTTTGATGTTTTGACCAGGAAGTGGACAAGGCTTAATCCGATTGGTGACGTTCCG TCTCCCAGGGCTTGTCATGCTGCCGCTTTATATGGCACCTTGATCCTTATTCAGGGTGGGATTGGTCCATCCGGACCTTC TGATGGAGATGTCTACATGCTTGACATGACTAATAACAAGTGGATTAAGTTTCTGGTTGGAGGGGAGACTCCAAGCCCTC GCTATGGCCATGTTATGGATATAGCTGCTCAGAGGTGGCTTGTCATATTCAGCGGGAATAATGGAAATGAAATTCTGGAT GATACATGGGCATTGGACACGCGTGGACCATTTTCATGGGACAGATTGAACCCGTCTGGCAATCAGCCAAGTGGGAGAAT GTATGCTTCAGGTAGTTCTCGAGAAGATGGTATTTTTTTGCTCTGCGGTGGAATAGATCACTCAGGAGTGACACTGGGAG ATACTTACGGGCTGAAAATGGATAGCGATAATGTTTGGACTCCTGTTCCAGCTGTAGCTCCTTCCCCGAGATATCAACAT ACTGCGGTTTTCGGAGGTTCAAAACTGCATGTCATTGGGGGTATACTGAATAGGGCTCGCCTAATCGATGGTGAAGCAGT TGTTGCTGTGCTTGATACTGAGACTGGTGAATGGGTAGATACCAATCAACCAGAGACCTCTGCGAGCGGCGCCAATAGAC AAAATCAATATCAACTTATGCGACGTTGTCACCATGCTGCTGCATCGTTTGGCAGCCACCTATATGTGCATGGCGGGATT AGAGAAGATGTATTGCTCGACGATTTGCTAGTTGCTGAAACATCCCAGTCTTCAAGTCCTGAACCAGAAGAAGATAATCC TGATAATTATATGTTGCTCGACGACTATCTAATGGATGAACCTAAGCCTTTAAGTTCTGAACCAGAAGCTTCATCCTTTA TAATGAGGAGCACAAGTGAAATTGCGATGGATAGATTGGCAGAGGCACATAACTTACCAACTATCGAAAATGCTTTTTAT GATAGTGCTATTGAAGGATACGTTCCACTTCAACATGGAGCTGAGACGGTTGGAAATCGAGGTGGCTTGGTGAGAACTGC AAGCTTGGATCAATCTACTCAAGACTTGCATAAGAAGGTCATTTCGACACTGTTGAGACCTAAAACCTGGACGCCTCCTG CCAACAGAGATTTTTTCTTGAGTTATTTAGAAGTTAAGCATTTGTGCGATGAGGTAGAGAAAATATTTATGAATGAACCA ACACTTCTTCAGCTCAAAGTTCCAATCAAAGTCTTCGGCGATATCCATGGACAATACGGGGATTTGATGCGTTTATTTCA TGAATATGGACATCCTTCAGTGGAAGGAGATATCACCCATATAGACTATTTGTTTTTGGGAGATTATGTTGACCGTGGAC AACACAGCTTGGAGATCATCATGTTGCTGTTTGCTTTGAAGATAGAATATCCGAAGAACATCCACTTGATTCGTGGAAAT CATGAGTCTCTTGCAATGAATAGAATTTATGGTTTTCTTACAGAATGCGAAGAAAGAATGGGCGAGTCCTATGGGTTCGA AGCATGGTTAAAGATTAATCAAGTTTTTGATTATCTTCCGCTTGCTGCACTTCTAGAAAAGAAAGTTTTGTGTGTCCATG GCGGAATTGGGAGGGCAGTTACGATCGAAGAAATTGAAAATATCGAAAGGCCGGCATTCCCGGATACTGGATCAATGGTC TTGAAAGACATCTTATGGTCAGATCCTACGATGAATGACACTGTTTTGGGTATAGTAGACAATGCCAGAGGAGAAGGTGT TGTCTCATTTGGGCCTGATATAGTCAAGGCATTTCTAGAAAGGAATGGTCTGGAGATGATACTAAGAGCACACGAGTGTG TTATTGACGGCTTTGAAAGATTCGCTGACGGACGGCTTATAACAGTCTTTTCTGCTACAAATTACTGTGGAACTGCACAA AATGCGGGAGCAATATTGGTAATTGGTAGAGATATGGTAATTTATCCTAAGCTGATTCATCCTCACCCTCCTCCCATCTC ATCTTCAGAAGAAGACTATACAGACAAAGCATGGATGCAGGAGCTGAACATCGAGATGCCGCCTACTCCAGCTCGAGGGG AGTCAAGTGAATAAAGGCAGATATTTGGTATAGAAGTTGTGCGGTTGAAGAGGTGTAAATGTTTCATTTGCAAGCTGCAC AAGTGTACCGGAACCTTTTTCTTTTCATTTCTT ................................................................................ ..........................................N..................................... .......................................................i........................ ...............................i.......................................N........ ......................N.....................i................................... ................................................................................ i.............................N................................................. ................N..............N................................................ ..N.....N.......i........i...................................................... ...N.....N....i.............................................N.....N...........N. .....i.............................N..........................................i. ..N.......................N..................................................... .................i............N................................................. ............................N......................................N............ ..............................N................................................. .................i...............N.............................N.....N.......... .......N........................................................................ ..........i....................i...N............................................ ..i.......................i..........................................N........N. ..................................N............................................. ................................................................................ ................................................N...................N...N....... ..............................................N...................i............N .....N...........................................................N.............. ....................N........................................................... ...N.........................................................................N.. ..........................................................................i..... ..............N...............N...N..........................i.................. ............................................N..........N........................ ................................................................................ ..............................i...i....................N........................ ..........................................................N..................... ................................. Pos Score Pred ------------------------ 123 0.176 - 216 0.581 Yes 272 0.718 Yes 312 0.484 - 343 0.368 - 365 0.608 Yes 481 0.515 Yes 511 0.115 - 577 0.236 - 592 0.215 - 643 0.277 - 649 0.085 - 657 0.697 Yes 666 0.783 Yes 724 0.268 - 730 0.196 - 735 0.624 Yes 781 0.477 - 787 0.434 - 799 0.224 - 806 0.544 Yes 836 0.307 - 879 0.671 Yes 883 0.108 - 907 0.301 - 978 0.818 Yes 991 0.292 - 1069 0.172 - 1108 0.389 - 1151 0.362 - 1218 0.792 Yes 1234 0.316 - 1264 0.158 - 1270 0.389 - 1288 0.332 - 1371 0.636 Yes 1392 0.736 Yes 1396 0.213 - 1443 0.690 Yes 1467 0.756 Yes 1510 0.224 - 1519 0.152 - 1555 0.120 - 1729 0.382 - 1749 0.478 - 1753 0.309 - 1807 0.201 - 1827 0.506 Yes 1840 0.248 - 1846 0.270 - 1906 0.232 - 1941 0.361 - 2002 0.218 - 2016 0.526 Yes 2029 0.235 - 2045 0.352 - 2058 0.731 Yes 2071 0.186 - 2084 0.334 - 2158 0.324 - 2235 0.572 Yes 2255 0.172 - 2271 0.463 - 2275 0.099 - 2302 0.609 Yes 2365 0.264 - 2376 0.431 - 2482 0.406 - 2514 0.485 - 2591 0.792 Yes 2595 0.674 Yes 2616 0.321 - 2699 0.075 -

Explain the output -- Processing locus 68: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGAGGTGAAGCAAAGTCGGGAGCTGTACATTTCCGCTTTCATGTTCTGTGTTGAATCCGAATTCTCTCCACATTGTGAGT TTCTCTTCGATTTGAATCTAACATTCTATTGTTGAAATTAGGAATTTTTTGATTTGTAACAAATGGGTGATTCTGGTTTT TGTATGGATGAAGAGTTGTTTTTACAGATCTCCGAAGTTGGCTCCTCAAAGAAGATCACAGCGCCTCATGGGCGGCCAAA TGCAGCAAAACAATGCTGCGGCTGCGACGGCGCTTTACGATGGGGCTTTACCCACTAATGACGCAGGAGATGCAGTCATG GCACGGTGGCTTCAATCCGCTGGTTTGCAGCATTTGGCGTCTCCTGTTGCTTCTACAGGCAATGATCAGCGTCACCTCCC AAACCTTCTCATGCAGGGTTATGGAGCTCAGACTGCTGAAGAGAAACAAAGACTGTTCCAACTAATGAGAAATCTCAATT TTAATGGGGAGTCGACTTCTGAATCATATACACCAACTGCTCACACATCAGCAGCTATGCCCTCTTCGGAAGGATTTTTT TCACCTGAGTTCAGAGGTGATTTTGGAGCAGGATTATTGGATCTTCATGCAATGGATGATACAGAGCTTCTATCTGAGCA TGTGATTACCGAACCCTTTGAGCCGTCACCTTTCATGCCTAGTGTAAATAAAGAATTTGAAGAAGACTATAATTTGGCAG CTAATCGTCAACAGCGGCAACAGACAGAAGCTGAACCTTTGGGTTTATTGCCTAAAAGTGATAAAGAAAATAACAGTGTA GCCAAGATTAAAGTAGTGGTAAGGAAAAGACCCCTAAACAAGAAAGAAACAGCTAAAAAGGAGGAGGATGTCGTGACGGT ATCTGATAATTCTTTGACTGTCCATGAGCCCAGAGTGAAGGTTGATTTGACTGCTTATGTGGAAAAGCATGAGTTCTGCT TTGATGCTGTTCTAGATGAGGATGTTTCAAATGACGAGGTGTATCGGGCCACAATTGAGCCAATAATTCCCATTATTTTC CAGAGAACTAAAGCTACATGCTTTGCATATGGCCAAACAGGTAGTGGTAAGACATTTACAATGAAACCATTACCTATACG AGCAGTTGAAGATCTTATGAGGTTGTTGCGTCAACCAGTATACAGCAATCAGAGGTTTAAATTGTGGCTCAGCTATTTTG AGATATATGGTGGAAAGCTGTTCGATCTTCTCAGTGAGAGAAAGAAACTTTGCATGCGAGAAGATGGTAGACAGCAAGTT TGCATTGTTGGCCTGCAAGAATATGAAGTTTCAGATGTACAAATTGTAAAGGATTTTATCGAGAAAGGAAATGCCGAAAG GAGCACAGGTTCAACTGGAGCAAATGAGGAATCTTCTAGATCGCATGCCATCCTACAGCTTGTTGTAAAAAAGCATGTTG AGGTAAAAGACACTAGACGGAGGAATAATGATAGTAATGAATTGCCTGGGAAAGTTGTGGGAAAGATTTCTTTCATTGAC CTTGCTGGCAGTGAAAGAGGTGCAGACACCACAGACAATGATCGCCAGACAAGGATTGAAGGCGCAGAAATCAACAAGAG TCTCTTGGCTCTTAAGGAATGTATACGTGCACTGGACAATGACCAGCTACATATACCATTTCGTGGAAGCAAACTAACGG AAGTGCTCCGTGACTCATTTGTTGGAAACTCAAGAACGGTGATGATTTCCTGCATCTCTCCAAATGCAGGATCGTGTGAA CATACCCTCAATACTCTAAGATATGCTGATCGAGTCAAAAGTCTATCTAAAAGTGGAAATAGCAAGAAAGATCAAACTGC GAATTCAATGCCTCCGGTTAATAAGGATCCTTTGTTGGGCCCAAATGATGTAGAAGATGTCTTTGAGCCTCCACAGGAAG TGAATGTACCAGAAACCAGGAGGAGGGTGGTCGAGAAGGACAGCAACAGCAGTACGTCGGGTATTGACTTCAGACAGCCT ACAAATTATCGAGAGGAAAGTGGAATCCCATCATTCTCAATGGACAAGGGAAGATCAGAGCCGAACAGTTCTTTTGCTGG CTCCACTAGTCAGAGAAACAACATTTCTTCATATCCCCAAGAAACTTCAGACCGTGAAGAGAAAGTAAAGAAAGTGTCAC CACCTCGTGGGAAAGGGTTGCGGGAAGAAAAACCAGACAGACCACAAAATTGGTCTAAAAGAGATGTCAGTTCGTCGGAT ATCCCTACCTTGACAAATTTTAGACAGAACGCAAGTGAAACTGCTTCAAGGCAATATGAAACCGCTTCAAGGCAATATGA AACCGACCCTTCGCTTGATGAAAACCTCGATGCACTGCTTGAGGAAGAAGAAGCTCTGATTGCAGCGCACAGAAAAGAAA TTGAGGATACAATGGAGATTGTTCGCGAGGAAATGAAACTTCTAGCGGAGGTGGACCAACCGGGAAGCATGATAGAAAAC TATGTGACGCAACTGAGCTTTGTGTTGTCCCGGAAAGCAGCAGGGCTAGTCAGTCTTCAAGCCAGGCTTGCTCGGTTCCA ACACCGTCTCAAGGAACAAGAAATACTGAGCCGTAAGAGAGTTCCTCGGTAGTTGGTAGAAGAAGGAAAACCAAAAGTGT GGTGTTGTTTAAGTGTGAAAGGTTGGTTGGGTGATCTCTTCTTATATATGACAAAGAATGACAGAAATGTC ........................................N....................................... ..............................................................i................. ...i...i...........................................................i...........i ............i..........................N.................N...........N.......i.. .............................................................N.................. ..........i.........N...........................................N............... ...N....................................................i....................... ..............................................N....i...N.......................N ..................................i............................................. ................................................................................ ...................................................................N............ .......................N................................N...........N........... ...N...........N.....N........N................................................. .................N..........i...............................N................... ................N............................................................... ......i..............................................i.........i................ ......................N...........N...................................N......... .......................N....................N.............................N..... ...........................N........N........................................... .....................................N.......................................... ..................N...................N......................................... .........................................N.....................i................ ......................N......................................................... .......i....................................N..N........N....................... ...N............................................................................ .......................................i........................................ ................................................................................ ...............................................................i................ .......................................................N....................N... .................N...........N.................................................. ...........i....................N...................................N........... ................................................................................ ...............................................N.........N........N.... Pos Score Pred ------------------------ 41 0.123 - 143 0.804 Yes 164 0.556 Yes 168 0.621 Yes 228 0.578 Yes 240 0.648 Yes 253 0.522 Yes 280 0.285 - 298 0.199 - 310 0.308 - 318 0.793 Yes 382 0.088 - 411 0.585 Yes 421 0.220 - 465 0.433 - 484 0.431 - 537 0.665 Yes 607 0.135 - 612 0.773 Yes 616 0.141 - 640 0.460 - 675 0.624 Yes 868 0.188 - 904 0.181 - 937 0.061 - 949 0.220 - 964 0.186 - 976 0.151 - 982 0.162 - 991 0.266 - 1058 0.279 - 1069 0.584 Yes 1101 0.180 - 1137 0.205 - 1207 0.568 Yes 1254 0.709 Yes 1264 0.578 Yes 1303 0.214 - 1315 0.322 - 1351 0.369 - 1384 0.258 - 1405 0.156 - 1435 0.330 - 1468 0.460 - 1477 0.185 - 1558 0.314 - 1619 0.247 - 1639 0.209 - 1722 0.318 - 1744 0.677 Yes 1783 0.232 - 1848 0.803 Yes 1885 0.226 - 1888 0.289 - 1897 0.262 - 1924 0.207 - 2040 0.765 Yes 2224 0.517 Yes 2296 0.204 - 2317 0.251 - 2338 0.085 - 2350 0.113 - 2412 0.586 Yes 2433 0.244 - 2469 0.415 - 2482 0.506 Yes 2688 0.050 - 2698 0.065 - 2707 0.068 -

Explain the output -- Processing locus 69: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGCACATCGATTAACATATCATCGACTTTGGAACCTCACAACATAATCGCATGTAGTCACACTTAGTCAAATCCAAACCC AAAAAATCTCAAAGAAAATAAGAAAAAATAAAAATAAAAATGAAGAGAATAAGAATAATGGCAACGCTTGTAATAGCAAA CATGTTAATGGCGACTCCAACGTCTCGAGCCTCTCTCAATCTTCTTCGTCGATCGCCAAAACCCAAATATTTCTCATCTT CTTCTTGTCATTTTCGTCCCTCAACTTTTCGTAAATCATATCCTTGCCCCATCTGGTCTTCTTCTTTCTCTTTTTGTCTC CCTCCTCCTAGATCCACCACTTCCACCTCTCTCTCCTCCTCCTCTTTCCGCCCCTTCTCCTCTCCTCCGTCCATGTCTTC CGCCGCCGCTGCCGCCGTGGAATCCGTCGTCTCCGACGAGACTCTTTCCTCGAATCCTCTCTTGCAGGACTTCGATTTCC CTCCGTTTGATTCCGTTGATGCCAGTCATGTTCGTCCTGGGATTCGTGCTCTATTGCAGCATCTCGAAGCTGAATTGGAG GAGCTAGAGAAATCTGTGGAGCCAACATGGCCAAAACTGGTGGAACCATTAGAGAAAATCGTTGATCGGTTAACTGTTGT TTGGGGAATGATCAATCACCTTAAGGCTGTCAAGGACACACCTGAGCTACGTGCTGCCATCGAAGACGTTCAGCCAGAGA AAGTGAAGTTCCAGCTCAGGTTGGGGCAAAGCAAGCCTATTTACAATGCCTTTAAAGCTATTCGTGAATCTCCTGATTGG TCATCGCTCAGTGAAGCTCGTCAACGCTTAGTAGAAGCTCAAATAAAGGAGGCGGTTCTCATTGGTATTGCTCTTGATGA TGAAAAGAGAGAAGAGTTTAATAAAATTGAACAGGAACTCGAAAAACTTTCCCATAAGTTTTCTGAGAATGTTTTGGATG CAACAAAGAAATTTGAAAAGTTGATAACAGACAAGAAAGAGATCGAGGGATTGCCTCCATCTGCTCTTGGGCTATTCGCA CAAGCAGCTGTCTCCAAGGGCCATGAAAATGCAACTGCTGAGAATGGACCATGGATCATTACACTGGATGCTCCTAGTTA TCTTCCTGTCATGCAACATGCTAAAAACCGTGCTCTGCGTGAGGAAGTCTACCGTGCTTACCTATCTCGTGCCTCTTCTG GCGATTTGGATAATACGGCAATTATTGACCAGATTTTGAAGCTTCGCTTGGAAAAAGCTAAGCTTCTTGGTTACAACAAT TACGCTGAGGTAAGCATGGCTATGAAAATGGCTACTGTTGAGAAAGCAGCAGAGCTTTTAGAGAAGCTTCGTAGTGCATC CTGGGATGCGGCTGTTCAAGACATGGAAGACCTCAAGAGTTTCGCAAAGAACCAGGGTGCTGCAGAATCTGACAGTATGA CTCATTGGGACACCACTTTCTGGAGTGAGAGGCTTCGTGAATCCAAATACGATATTAATGAGGAAGAACTACGTCCATAC TTCTCACTGCCAAAGGTTATGGATGGACTTTTCAGTCTAGCTAAGACACTTTTTGGAATTGATATTGAACCAGCAGATGG TTTAGCTCCGGTCTGGAACAATGATGTTCGGTTCTACCGCGTCAAAGATTCTTCTGGGAACCCAATCGCTTACTTTTACT TTGATCCATACTCTCGCCCGTCAGAGAAAAGAGGTGGCGCTTGGATGGATGAGGTTGTTTCCCGTAGCCGAGTCATGGCT CAGAAAGGCTCTTCTGTTAGGCTGCCTGTTGCACACATGGTCTGCAACCAAACTCCACCAGTAGGTGACAAGCCAAGCCT TATGACATTCCGTGAGGTAGAGACAGTATTTCATGAATTTGGACATGCTCTTCAGCACATGCTTACGAAGCAGGATGAGG GGCTAGTTGCTGGTATCCGAAATATCGAGTGGGACGCGGTTGAATTACCATCACAGTTTATGGAGAATTGGTGCTACCAC AGGGACACTTTAATGAGCATTGCTAAGCATTACGAAACAGGAGAAACACTCCCAGAGGAAGTATACAAGAAACTCCTGGC CGCAAGAACATTCCGTGCAGGGTCCTTTAGTCTTCGTCAGTTGAAATTTGCCAGTGTTGATCTTGAGCTGCACACAAAAT ACGTACCAGGAGGACCAGAGTCCATCTATGACGTAGATCAAAGGGTATCAGTAAAAACCCAAGTTATCCCTCCACTTCCT GAGGATAGATTTCTCTGTAGCTTCAGTCACATCTTTGCCGGGGGATATGCAGCTGGATATTACAGTTACAAGTGGGCAGA AGTTTTGTCTGCTGATGCATTTTCAGCATTTGAAGACGCTGGACTAGACGACATCAAGGCTGTTAAAGAAACAGGACAGA GATTCAGAAACACCATACTTGCACTTGGAGGAGGGAAAGCGCCTCTTAAGGTGTTTGTTGAGTTTAGAGGACGAGAACCT TCACCCGAGCCTCTTCTCAGACACAATGGACTCTTGGCTGCCTCTGCTTCTGCTTAACTCTTATATAATCCTCACAACAT TTGGAAACTTTGAAAGACATTTATAAGATGGAAAATCAACTTTTCTTTTTTAATATGCCAACCATTTACATCTAAT ..................................................N............................. .......................................i.................i...................... ................................................................................ ........................................................................i....... ................................................................................ ..................N........N.................................................... ..........................i..................................................... .......i........................................................................ .............................................N.................................. ............................................................................N..N ....................................................................N........N.. ................................................................................ ......................N.....N..............N......N................N............ ..........i......N.............................................................. ................................................................................ ...............i.....i.....i.................................................... .....N................i.....................................................N... .........................................................N...................... ..................i...i.....................................................N... ....................i..N........................................................ ............................................i...N.........................i..... ....................................N........................................... ...........................................................N.................... ............i................................................................... ................................................................................ ...........................N.................................................... ..............................................N................................. ..............N................................................................. ................................................................................ .........................N...................................................... ...........................N..........................N..................... Pos Score Pred ------------------------ 51 0.348 - 120 0.635 Yes 138 0.718 Yes 162 0.247 - 168 0.238 - 393 0.820 Yes 499 0.164 - 508 0.287 - 587 0.691 Yes 648 0.569 Yes 766 0.435 - 877 0.086 - 880 0.194 - 949 0.152 - 958 0.155 - 1063 0.173 - 1069 0.233 - 1084 0.364 - 1091 0.436 - 1108 0.154 - 1131 0.729 Yes 1138 0.225 - 1296 0.819 Yes 1302 0.588 Yes 1308 0.860 Yes 1366 0.290 - 1383 0.752 Yes 1437 0.385 - 1498 0.307 - 1539 0.621 Yes 1543 0.664 Yes 1597 0.461 - 1621 0.676 Yes 1624 0.421 - 1725 0.606 Yes 1729 0.299 - 1755 0.816 Yes 1797 0.422 - 1842 0.528 Yes 1873 0.260 - 1885 0.283 - 1899 0.537 Yes 1915 0.311 - 1980 0.477 - 2013 0.614 Yes 2188 0.340 - 2287 0.193 - 2335 0.272 - 2506 0.239 - 2588 0.199 - 2615 0.060 -

Explain the output -- Processing locus 70: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATCTTAAATTTCACAAAAATATAAGTCCCAACATAACAATTTTTTCGAAAAAGACTTTTCTAATAAAGTAGGCGCTTTC AATTTTCACAACAGTCAATAAATACATCACCCACTTTTTCTATCCAAGTCAACTAAAAACGCGGGTCTTCTTCTCCAAAA CCCATTTCCTCACAAAATCCATCTGAGAAAAAGAGCAACAAGTTTCATTTTCATGGTGGTGATGACGAACAAATTCTTCG AATTAATCGGCGGAGCTCCGTCGTATTCTTCAGTATCCGTCGCTGTTAAAGGATCCGTCGGTGACGCCGTAGGAGGAACT GCTAGTCGTCGTGCTCTTCGTTGGACAATCGAAAATTTCCTTCCTAAAATCGATAGATTAGTTCTTGTTCATGTCATGCC TACAGTTACTACTATTCCATCTCCTTCTGGATCGAAGATTCCAATAGAGGAATTGGATGAGAGTGTTGTGTCTATGTATA AACGAGATTTGAGAAAAGAATTTGAACAAGTTTTTGTTCCGTTTAAGAGGATCTGCAAATCCAACAAAGTTGAGACTTTG TTGTTGGAACATCATGATCCTGCAAAGGCTCTTTTGAAGTATATGTCAGATACTGATGTTGAGTGTTTAGTGATTGGCTC TTGCTCTTCTAATTTCCTCACAAGGAAAAAAGGACAAGAAATGCCATTAACGGTGCTAGGAGAAGCTCCAGAGACATGTG AGATATATGTTGTTTGTAAAGATAGAATCTTAACCAAATCGACGAATCAGTTTACCGCAGATTCATCATCTAGTTTCCGT ATACCAGAAGGAGCTGAAGCTTATACAGAATCTTTCAGTCGCACACGCTCTGATAAGACAGGGTTGTCTGCTTCATCTAT AACATCTTCAGGCAGGATGAGAATTGGAAGGCCTGGCTCTTTACCGCATAGTCATCCGACCTCTCGAGTGTATTCTGATG CACAGTCTTCTTCAACTGATATTGTTTTGGTCGATGACGAACACTGTCGTTCTATCCTTAGGCATAGTACTGTTTCCACT AGTAAAATACAGATGGATCCTAGACCTCACTTAAAAACACCAAAGTCAGGTGTAAGAGCTGAGGTAGAGCAGCTAAGGAA AGAAGTGCAAACCACTCTTTCCATGTACAAACAAGCTTGTGAAGAGCTAGTTCATAAACAAACACAGGTCCAGTCTCTTT CCTCTGAGTGTATCAAAGAAACAGAAAGGGTCATCACTGCTCTGGAAAAAGAAGAAATGCGAAGGAAAGCAGCAGCAGAA GAGAAAGAAAAGCATCTAAAAGCTGTAAAAGAAGTCGAGGAAGCAAAATCAATGCTAGCCAAAGAGTTCTGCGAGAGGCA ATTAGCCGAGCTAGATGCTCTCAAACAGTCCATAGAGAAACAGAAAGTCATTGAGCAACTCTTCTTGAGAGACGGGCGAT ACAGAAAGTACACAAAAGAAGAAATAGCTGCAGCTACAGATAATTTCTCTTCCCGTAAAATAATCGGCGAAGGAGGATAT GGAAAAGTATACAAATGCAGTCTTGATCATACCCCAGTAGCTCTTAAAGTTCTCAAACCTGATTCTGTTGAAAAGAAAGA GGAATTCTTGAAAGAGATCTCAGTCTTAAGCCAGCTTCGTCATCCCCATGTGGTTCTTCTCCTTGGTGCTTGTCCTGAAA ATGGATGCCTTGTCTATGAGTACATGGAAAATGGTAGCTTAGATTGTCACATCTCTCCCAAAAAAGGGAAACCATCTCTC TCATGGTTTATCAGATTCCGAATCATCTATGAAACAGCTTGTGGTTTAGCTTTCCTCCACAACTCTAAGCCTGAGCCCAT CGTCCACCGTGACCTCAAACCAGGCAACATTCTCTTAGACAGAAACTTTGTCAGCAAAATCGGTGACGTTGGACTCGCAA AACTCATGTCAGACGAAGCACCAGACAGTGTGACGGTTTACAGAAACTCGATTATCGCCGGTACACTCTACTACATGGAC CCGGAATACCAACGAACCGGCACAATCAGACCGAAATCAGATCTTTACGCATTTGGAATCATAATTCTCCAGCTTTTAAC CGCTCGACATCCCAACGGTCTTCTTTTCTGCGTTGAAGATGCAGTAAAGAGAGGCTGTTTTGAAGATATGTTAGATGGAT CAGTCAAAGACTGGCCCATAGCCGAAGCGAAAGAACTAGCTCGCATTGCAATCAGGTGTTCGCAGCTCAAATGCAGAGAC AGACCAGATCTTAGCACACAAGTCTTGCCTGCTCTCAAACGTATTCTTGAATCTGCAAATAGTAGACTCAAGACAGAACA AGCTAACGCACGAGCACCAACTCACTATTACTGTCCAATTCTTAAGGAAATAATGGAAGATCCACAAATAGCAGCAGATG GATTCACATACGAACGAAAAGCGATAAAAGAATGGATTCAAAAACATCAAGATGTGTCTCCTGTTACTAAGCATCGGCTC AAACATTCTGATCTTACACCGAACCACACTCTCAGATCGGCTATACGAGAGTGGCGATCTCGTTCACGCTTAGACCTCTC CACTACTCTTGGCTCCTACTGATCACACTAATATATATACAGTCTAGTCTAATTCGTATATTGACTAAATACCCTGAACT TGTTTGAATCGGGTGTATCATCTCTGTGAATATGTATTGTG ................................................................................ ................................................................................ ....................................................i........i.................. ................................................................................ ......................................................................N....i.... ........................................................N................N...... ................................................................................ .............N............................N............N........................ ........................................i..................................N.... ......N......................................................................... ................................................................................ ................i............................................................N.. .................................N.............................................. ............i................................................................... ......................N......................................................... ........................................................i....................... ...................................................i............................ ..............N................................................................. ..............................................................................N. ..............N................................................................. ...............................................N................................ N...N..........i.......i......i................................................. ..N.........................N................................................... ................................................................................ .....i....................................................................N..... ................................................................................ ......................................N............................i......N..... ......................................................................N......... ................................................................................ ....................................................i........................N.. ...............................i...................i............................ ................................................................................ ................................................................................ ...............................N......... Pos Score Pred ------------------------ 213 0.519 Yes 222 0.603 Yes 391 0.138 - 396 0.777 Yes 457 0.226 - 474 0.303 - 574 0.194 - 603 0.339 - 616 0.331 - 681 0.643 Yes 716 0.472 - 727 0.313 - 897 0.619 Yes 958 0.189 - 994 0.380 - 1053 0.794 Yes 1143 0.282 - 1257 0.807 Yes 1332 0.645 Yes 1375 0.270 - 1519 0.478 - 1535 0.267 - 1648 0.355 - 1681 0.457 - 1685 0.457 - 1696 0.513 Yes 1704 0.517 Yes 1711 0.642 Yes 1763 0.206 - 1789 0.372 - 1926 0.578 Yes 1995 0.330 - 2119 0.468 - 2148 0.625 Yes 2155 0.468 - 2231 0.347 - 2373 0.627 Yes 2398 0.340 - 2432 0.608 Yes 2452 0.510 Yes 2672 0.075 -

Explain the output -- Processing locus 71: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTTTCCGGATTTTATCCTTGTCCAATCGGAATAATCATAGGACTTTAACTTTTATCAACCGATTTCAGTAGATTTGATA GTTTATACTTTCTACAGCTGAGTTTTAACCAATGAATTAGGTCTAAAAGTTATCATGAACTTTGATGTTTCTGGTTGCAG AAAAATGTTCAGCTCGATGCAGATACTGCCACTGGAGGCTCCTCCTACGGATGGGAAGTTAGGTCCACTTCCTCCGTCGC AATTAACAGATCAGGAAGTCGAAGAGAGGGAGTTGCAAGCAGAGCAAAATAATTCAAATCTAGCTCCTCCGGCGGCTGTT GCTACTCACACGAGGACTATCGGAATCATTCATCCACCTCCAGATATCAGAACTATTGTTGAGAAAACGGCTCAGTTTGT CTCCAAAAACGGTTTGGAGTTTGAAAAGAGGATTATTGTTAGTAATGAAAAGAATGCTAAGTTCAACTTTTTGAAGAGCT CAGATCCTTATCATGCCTTTTACCAGCACAAGCTCACTGAATACCGTGCTCAGAATAAAGACGGAGCTCAGGGTACTGAT GATTCAGATGGTACTACAGATCCCCAACTTGATACTGGAGCTGCTGATGAGTCTGAAGCTGGTGATACACAACCCGACCT TCAGGCTCAATTTAGGATTCCTTCTAAGCCTTTGGAAGCTCCGGAACCTGAGAAGTATACAGTTAGACTTCCTGAAGGGA TCACGGGTGAAGAGCTTGATATTATTAAGCTCACAGCACAGTTTGTGGCACGGAATGGGAAATCGTTTCTAACGGGATTG TCGAATAGAGAGAACAACAATCCTCAGTTTCATTTTATGAAGCCAACACACAGCATGTTCACTTTTTTTACTTCTCTGGT TGATGCGTATTCGGAAGTATTAATGCCTCCCAAAGATTTGAAAGAAAAGCTGAGAAAGAGTGCTGCTGATTTGACGACTG TTCTTGAGCGTTGTTTGCATCGTTTGGAATGGGATCGTTCCCAGGAGCAGCAGAAGAAGAAAGAAGAGGATGAGAAAGAG CTTGAGCGAGTGCAGATGGCTATGATTGATTGGCATGATTTTGTGGTTGTTGAGTCGATAGATTTTGCGGACGAGGAAGA TGAAGAGTTGCCACCGCCTATGACACTTGACGAGGTTATAAGGAGGAGCAAAGCATCAGCCATGGAAGAAGATGAAATTG TTGAGCCTGGAAAGGAGGTCGAAATGGAAATGGACGAAGAAGAAGTTAAGCTTGTTGCAGAAGGAATGAGAGCAGCAAAC CTAGAAGAGAATGTTAAGATTGAAAATGTACATGATGAAGAAGCACCTATGAGAATTGTTAAGAACTGGAAGAGGCCAGA AGACAGGATCCCAACAGAGAGAGATCCAACTAAAGTTGTTATATCACCAATTACTGGAGAGCTGATTCCCATTAACGAGA TGTCTGAGCACATGAGGATTTCTCTTATTGATCCTAAGTTCAAAGAACAGAAGGATCGGATGTTTGCTAAGATTCGTGAG ACCACTCTTGCACAAGATGATGAGATTGCTAAAAACATAGTCGGGTTGGCCCGCCTGCGTCCTGATATCTTTGGAACAAC CGAAGAAGAAGTCTCAAATGCTGTAAAAGCAGAGATTGAGAAGAAGAAAGATGAGCAGCCCAAGCAAGTGATATGGGATG GTCACACAGGAAGTATTGGTCGCACAGCAAACCAAGCCTTGAGTCAGAACGCTAATGGAGAGGAGCAAGGTGATGGCGTT TACGGAGATCCCAATAGCTTCCCTGGTCCAGCTGCTCTTCCACCTCCTCGACCAGGTGTCCCAATAGTCCGACCATTGCC ACCACCTCCTAATCTCGCCTTGAACCTCCCTCGTCCTCCCCCTTCTGCTCAGTACCCCGGTGCACCAAGGCCACTAGGTG TTCCGATGATGCAGCCCATGCATCAACAACACCAGCTCACAATGCCAGGGCCACCAGGACACCCGCAGATGATGATGAAC CGACCACCACAGATGCAGCCTGGTATGCATGTGCCACCTCCACCGGGATCTCAGTTTGCTCATCATATGCAAATTCCTAG ACCTTATGGTCAGCTTCCACCATCTGCAATGGGGATGATGCAACCACCACCTATGCCTGGGATGGCTCCTCCTCCACCAC CCGAGGAGGCTCCACCACCTCTTCCTGAGGAACCAGAGGCAAAGAGACAAAAGTTCGATGAGTCAGCCCTTGTTCCAGAA GACCAGTTTCTTGCTCAGCATCCGGGTCCGGCTACAATCAGGGTTTCTAAGCCAAACGAGAATGATGGACAATTTATGGA GATCACAGTGCAGTCGTTATCTGAAAACGTGGGAAGTTTGAAGGAGAAAATAGCTGGGGAGATTCAAATCCCAGCAAACA AACAGAAGTTGAGTGGAAAAGCAGGGTTCTTAAAGGACAACATGTCGCTTGCACATTACAATGTCGGAGCAGGAGAAATC CTAACATTGTCTTTGAGGGAACGTGGTGGGAGAAAGAGATAGAGAGACAGCTGGTCTACGCAGGGAAATGCTTTTTGATC TCTTATTCTTCTCTAGATGTTTAAATTGATGGCCAAAACTATATGTACTACTCTCTCGTTC ................................................................................ ...............................N......................i.........N............... ....i...........N.................................N............................. ................................................................................ ................................................................................ ............................................N........N.......................... ............N.................................................................N. .......N......................................N................................. ................................................................................ ......................................................N......................... ....................................N.................i......................... ..N...................i......................................................... ............................N........................................N.......... ...............i.....N............N............................................N ...................i.........................................i.........N........ .......................i.....i...................................i.............. ..........N..............N.....N..N.............i............................... ...............................................................................i ...........i...............................................i.................... ................N..N............................................................ .................N................................i.....................N....N.. ......................................................N.................i....... ................................................................................ ................................................................................ .....N..i........N.......................i..........................N..i..N..... ............i...........i...N.....................................i............. .....N......................i.....i..i..............i........i.................. .........................................................N...................... .............................................................N..N..........N.... ................................................................................ .........................................i..................i................... ...................................................................N............ ................N...........N.............N.................. Pos Score Pred ------------------------ 112 0.253 - 135 0.606 Yes 145 0.164 - 165 0.726 Yes 177 0.482 - 211 0.240 - 445 0.273 - 454 0.407 - 493 0.177 - 559 0.149 - 568 0.234 - 607 0.199 - 775 0.113 - 837 0.343 - 855 0.505 Yes 883 0.133 - 903 0.698 Yes 989 0.455 - 1030 0.128 - 1056 0.765 Yes 1062 0.463 - 1075 0.131 - 1120 0.220 - 1140 0.826 Yes 1182 0.755 Yes 1192 0.104 - 1224 0.860 Yes 1230 0.811 Yes 1266 0.740 Yes 1291 0.104 - 1306 0.204 - 1312 0.073 - 1315 0.113 - 1329 0.660 Yes 1440 0.747 Yes 1452 0.606 Yes 1500 0.858 Yes 1537 0.234 - 1540 0.227 - 1618 0.270 - 1651 0.589 Yes 1673 0.468 - 1678 0.313 - 1735 0.354 - 1753 0.596 Yes 1926 0.491 - 1929 0.572 Yes 1938 0.429 - 1962 0.769 Yes 1989 0.304 - 1992 0.511 Yes 1995 0.403 - 2013 0.577 Yes 2025 0.537 Yes 2029 0.302 - 2067 0.590 Yes 2086 0.153 - 2109 0.861 Yes 2115 0.581 Yes 2118 0.560 Yes 2133 0.831 Yes 2142 0.640 Yes 2218 0.226 - 2302 0.398 - 2305 0.291 - 2316 0.479 - 2442 0.585 Yes 2461 0.689 Yes 2548 0.051 - 2577 0.044 - 2589 0.071 - 2603 0.034 -

Explain the output -- Processing locus 72: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCTCGTTGTAGTATACAGTTCCCAGGATTCTGACATAATGTTTCAACTCATGACATTTCTTAATTTGTGTAATCTAATG GTCGTTTTGACTACATAATGAATGCAGATGGCTAGATGGGATCAGATTTTCTCCTTACCTGTGCAAAATCCTACCTTGCC AGAATTTTCTTCTACCGATCTTGTCTGGTCAAAGGTTGAAGGTTACCGGGACAATATAGATAGGTTAGCCCTCATCCCAT ATACTAGGGTGGATGATTTTGTAAGAGGTGAATGCTCGAATAAAGATTGTCCTACAAGTTTCCATGTTGAAGCTAGACGA CGGAAAGCCAAAGGAAAAAAATATAAGCCAAAGGTGGATGGCATCTTGGAGTATATTCTGTATTGGTGTTCCTTTGGACC GGATGACAATAGGAAAGGCGGAACTGTACGGCCAAGTAGGAGTACGTATGTTCCGAAAAAGAACAATGCAGGCCGACCCA ATTCTAAGAGAGGGTGCAGGTGTCACTTCATTGTGAAACGCTTAATTGCAGAACCGACTGTAGCATTAGTAATTTATAAC AATGACAAGCATGTAGATGAAAAAGGGTTTCCGTGCCATGGTCCACAAGACAAAAAGGCTGCTGGAACAAGAGCCATGTT TGCTCCATATATATCAGAAGACCTTAGGCTACGTGTATCATCTTTGCTCTATGTCGGTGTCTCTGTGGAGACCATAATGC AAAGGCATAATGAATCAGTGGAAAAACAAGGTGGTCCAAGTAACCGAGATGATCTGTTAACTCATAGATATGTACGAAGA CTTGAGAGGAGCATTCGACGTTCAACATATGAGCTCGATGAGGATGATGATGTCAGCATCAGCATGTGGGTTGAAAGTCA CCAAAGTCATGTGTTTTTCTTCGAGGGGTTCTCTGATACAGACCCTTTTTCCTTGGGTATCCAAACTGAGTGGCAGTTGC AACAAATGATTAGATTTGGAAACTGTAGACTTTTGGCTTCAGACTCAAGGTTTGGGACAAATACACTAAAGTATCCTATC CATAGTCTTGTTGTATTCGACTCTGAGAATAAGGCTATTCCAGTTGCTTGGATAATTGCACCGCGGTTTTCTAGTGGAGA TGCATACAGATGGATGAGAGCTCTTTGTAACCGAGTCCATGCCAAGGATCCCTCCTGGAAGGTGGCTGGGTTCATAGTTG ATGATCCTTTTGCTGACATAATAGCGATCAGGGATGTATTTCAGTGTCCAGTCTTGTTTTCCTTTTGGCGTCTTCGCCAT GCATGGCATAAGAACATAATCAAGAGATGTCGAGAAACAAAGACACGCGTTGAGATTTCAAGACATCTTGGGCAGGCTGT AGATAAAATCTCTAGAAGGCAAGGAACAGCCACATTGTTTGACAGTTTTGTGGAGGATTTTGTTGGTAGTCCAGAATTTG TGGAGTACTTCAGATCTGTTTGGTCTCCCAGAATAGGAGCCTGGACTAGTGCGTTACAATCTCTTCCCTTGGCAAGCCAA GAGACCTGTGCAGCGATGGAGCTTTACCACTATCAGCTGAAGTGCAGGCTTTTGAATGAGAGGGACTCTGAAGCTTATCA ACGTGCTGATTGGTTGGTTGATAAGTTAGGGACAAAAGTACATTCTTACTTCTGGCTTGACGAATATTCTGGAAAGGATA ATTTTGCCAGGTATTGGAAAGAGGAATGGGTGAGCGGGTTGACTTCCTTCCGCAAAGCATTGAGTATACCAGATTCTGAT GTTGTTATCTCTGGTATGTCTGCAAAAATAACAGATGAGTGTGACGGCAACGAAATTCATGTTGTATGGAATCCAGGGTC ACAGTTTGGGGTTTGCAGTTGCAGCTGGGCAGAAAAGGGATACATATGTAAGCACATGATCAAGCTTACACAACTCTGTC TAGGAAACCGAGCTGCCAGGCAATCTGCGAGCCTTCTACAGTATTATCAGACTTTGATTGATCTGCTACGCTGCCCACCT CACGATTCCTTGTTTCGTGATTATGCAGTCTCTTTAGCTGTCTCTGTGGAAAAGCAAATAAACGCACTCGGCTATCTGCA AAAGAGTGATGCTAATGAAGGTAACTTGCAGAAAGAAATTGCTTTCAGTGAACCATCTAGTGGAAAATCTCTTGATGAAT CTGACTTGCTAGATAAGCATGAGGGTCATGGTGAAGTTGCTACTGATTTAGATGGCGGATTGAGTAAAATGCCAATGAGT TGTCTGAGGGTCTGCTCAGGAAATGCAGAAGACATTATTTCGGGTTCTGAAATGGAAATTGGACTTTCACCAGGTTCAAC CAAAGCTGCTGCAGAAGATGTTACCTCTACTGTTCAGAATGGTACAGACAGTGCGAACGAGAATTGTGAAAGAGGTGCGA AAAGGTTGAAAATTTGGGCACCGGAGAACGGAGTGTATGAATGATGATGATAACTGAGCCGAGAACCTAGCTAGCGAGTA TTTCCAAGATTTGAATGAGCAAAATCTAACGTTGAGCACACAAACTGAGAAGCAACATCTACG ......................................N...........N..........................N.. .................N...N.....i.......N............................................ ................................................................................ ............N..................N...............................N................ .....................................N.......................................... ..N............................................N.................i.............. ................................................................................ ..................................................N.........................N... .........N......................................N....................N.......... ............................N........N.....N..N..N.............N................ ........N....................................................................... .....N.......................................................................... ...............................................................................N .........i...i........................N......................................... N................................N............................................i. ..i.......................N..................................................... ................................................................................ ................................................................................ ...............i.......................................i........................ ................................................................................ .........................i....................................................N. ...............N..................i.......................N......N.............. .............................................N.........N........................ ................................................................................ ......................N......................................................... ........N.....N...........................................................N..... ..................N........N.......................N................i.....i..... ......................N............................i............................ .................N....................N......................................... ....................................i...N..N..N................................. ..............N................................................ Pos Score Pred ------------------------ 39 0.330 - 51 0.179 - 78 0.248 - 98 0.378 - 102 0.475 - 108 0.592 Yes 116 0.387 - 253 0.299 - 272 0.211 - 304 0.198 - 358 0.470 - 403 0.308 - 448 0.412 - 466 0.506 Yes 562 0.773 Yes 571 0.667 Yes 577 0.203 - 598 0.216 - 636 0.624 Yes 691 0.164 - 717 0.401 - 730 0.263 - 769 0.184 - 790 0.292 - 829 0.295 - 838 0.178 - 844 0.446 - 847 0.133 - 850 0.390 - 864 0.459 - 889 0.334 - 966 0.438 - 1120 0.215 - 1130 0.697 Yes 1134 0.867 Yes 1159 0.137 - 1201 0.143 - 1234 0.208 - 1279 0.592 Yes 1283 0.644 Yes 1307 0.463 - 1536 0.753 Yes 1576 0.557 Yes 1706 0.522 Yes 1759 0.103 - 1776 0.463 - 1795 0.852 Yes 1819 0.342 - 1826 0.421 - 1886 0.379 - 1896 0.248 - 2023 0.272 - 2089 0.325 - 2095 0.472 - 2155 0.311 - 2179 0.164 - 2188 0.278 - 2212 0.221 - 2229 0.730 Yes 2235 0.800 Yes 2263 0.271 - 2292 0.844 Yes 2338 0.264 - 2359 0.388 - 2437 0.633 Yes 2441 0.241 - 2444 0.196 - 2447 0.148 - 2495 0.049 -

Explain the output -- Processing locus 73: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGTTGTTTTATTCGAAGATTCGTCGTTTGTTTTCAATCTCTGTTTTCTCATAGTGTAACTTGAAGCTGACAATTAGATTT GAATGATTTTGATCTCTTTGACAGTGGAAGATAATGGGGAAAGGAGGTGAAGATTGCGGGAATAAGCAAACTAATAGTTC TGAATTGGTCAAGTCTGATACTTTTCCTGCTTGGGGTAAAGATGTGTCGGAATGCGAAGAGAAGTTTGGTGTTAGTCGTG AGAAAGGTTTGAGCACTGATGAGGTGTTGAAACGGCATCAGATCTATGGTTTGAATGAATTAGAGAAGCCTGAGGGTACT TCTATTTTTAAGCTGATTTTGGAACAGTTTAATGATACATTGGTTCGTATTCTTTTGGCGGCTGCTGTTATTTCCTTTGT CTTAGCCTTCTTTGATGGCGACGAAGGTGGTGAGATGGGTATCACCGCATTTGTTGAGCCGCTTGTTATTTTTCTCATCT TGATTGTTAATGCCATTGTTGGCATCTGGCAAGAAACTAATGCTGAGAAGGCGTTGGAAGCTTTGAAAGAGATTCAGTCT CAGCAAGCTACCGTCATGCGTGATGGGACTAAGGTTTCCAGTTTGCCTGCAAAGGAGCTTGTTCCTGGTGATATTGTGGA ACTGAGGGTTGGTGATAAGGTGCCTGCTGATATGCGTGTGGTTGCTTTGATCAGTTCAACTTTGAGAGTCGAGCAGGGGT CCTTAACAGGAGAGAGTGAGGCAGTGAGCAAAACCACCAAGCACGTGGATGAGAATGCTGATATTCAAGGAAAGAAATGC ATGGTGTTTGCAGGAACCACTGTCGTGAATGGGAACTGCATATGTTTAGTTACAGATACTGGGATGAATACTGAGATTGG AAGGGTTCACTCACAGATCCAGGAAGCTGCACAACACGAGGAAGATACCCCTCTGAAGAAAAAACTTAACGAGTTTGGGG AAGTTCTGACTATGATCATTGGGTTAATTTGTGCGTTAGTGTGGCTCATCAATGTCAAATACTTTCTGTCATGGGAGTAT GTTGATGGCTGGCCCAGAAACTTTAAGTTCTCCTTCGAGAAGTGCACATATTACTTTGAGATTGCTGTTGCACTGGCGGT TGCTGCAATTCCAGAAGGTCTGCCAGCAGTTATTACCACATGTTTGGCTCTTGGCACTAGGAAGATGGCTCAGAAGAATG CTCTGGTTAGGAAGCTACCCAGTGTGGAGACTCTTGGATGCACCACCGTGATATGCTCTGACAAAACTGGAACTCTTACA ACCAATCAGATGGCTGTTTCAAAGCTCGTTGCTATGGGTTCGAGGATCGGAACTCTTCGATCTTTCAATGTTGAGGGGAC CTCGTTTGATCCTCGAGATGGAAAGATTGAAGATTGGCCAACGGGTCGGATGGATGCAAATCTTCAGATGATTGCAAAAA TTGCTGCCATTTGCAACGATGCTAATGTTGAGAAATCTGATCAGCAATTTGTGTCTAGGGGCATGCCTACTGAGGCAGCT TTGAAGGTTTTGGTTGAGAAAATGGGATTTCCCGAAGGATTGAATGAAGCTTCATCCGATGGCAATGTCTTACGTTGTTG TCGGCTATGGAGTGAACTAGAACAACGGATTGCCACCCTTGAGTTTGACCGTGACCGGAAATCAATGGGAGTTATGGTGG ATTCCAGCTCAGGAAAGAAACTGCTACTGGTCAAGGGTGCCGTAGAAAATGTATTGGAAAGGAGTACCCATATTCAGCTA CTTGATGGTTCCACTCGAGAGCTTGACCAATACTCAAGGGATCTTATTTTACAAAGCCTACATGATATGTCCTTGAGTGC ATTAAGATGTCTCGGATTTGCATACTCAGATGTTCCATCAGATTTTGCCACTTATGATGGCAGTGAAGACCATCCTGCTC ATCAGCAACTTCTCAACCCATCCAATTATTCATCAATTGAGTCCAATCTAGTATTTGTTGGATTTGTTGGTTTAAGGGAT CCTCCAAGGAAAGAGGTGCGTCAAGCCATTGCAGACTGCAGAACAGCAGGTATTCGGGTCATGGTTATCACCGGAGACAA CAAGAGCACAGCGGAAGCAATCTGCCGTGAAATTGGCGTATTTGAAGCAGATGAAGATATCTCCTCGAGAAGCTTGACAG GAAAAGAATTTATGGATGTTAAAGATCAGAAGAATCATCTAAGACAGACAGGAGGGCTCCTGTTCTCAAGGGCTGAACCA AAACACAAACAAGAGATTGTCAGGTTACTGAAAGAAGATGGAGAAGTGGTTGCCATGACTGGAGATGGAGTCAATGATGC TCCTGCCCTGAAGTTGGCTGATATTGGTGTGGCTATGGGCATTTCCGGGACAGAGGTAGCAAAGGAGGCATCTGACTTGG TGTTGGCAGATGATAATTTCAGCACCATTGTTGCAGCTGTTGGTGAAGGCAGGTCCATCTATAACAACATGAAGGCCTTC ATCAGGTACATGATCTCTTCCAACATCGGTGAGGTTGCATCGATATTCTTGACAGCTGCTCTTGGAATCCCAGAAGGCAT GATACCCGTTCAACTTCTGTGGGTGAATCTCGTAACAGATGGTCCTCCAGCTACTGCTTTGGGATTCAACCCCCCTGACA AGGATATTATGAAAAAGCCTCCTCGTAGGAGCGATGACTCTCTTATCACTGCGTGGATCCTCTTCCGCTATATGGTGATT GGTTTGTATGTGGGAGTAGCAACGGTGGGAGTATTCATCATATGGTACACACACAACAGCTTTATGGGCATAGACCTGAG CCAAGACGGTCACAGCCTTGTGAGCTATTCACAGCTCGCACATTGGGGCCAATGCTCTTCATGGGAAGGCTTCAAAGTGT CTCCTTTCACAGCAGGCTCTCAGACTTTCTCGTTCGACTCAAACCCTTGCGACTATTTTCAGCAGGGTAAAATCAAGGCA TCCACACTCTCCCTCTCGGTGTTGGTGGCCATTGAAATGTTCAACTCTCTGAATGCACTCTCAGAAGATGGCAGCCTGGT GACGATGCCACCCTGGGTTAACCCGTGGCTGCTCCTGGCAATGGCTGTCTCATTTGGACTCCACTTTGTGATATTGTACG TCCCATTCTTGGCTCAGGTGTTTGGTATTGTGCCACTGAGCTTGAACGAGTGGCTGCTAGTGCTGGCTGTGTCGCTGCCG GTAATTTTGATAGACGAAGTGCTCAAGTTTGTAGGAAGGTGCACGAGTGGCTACCGCTACTCACCTCGCACTCCCTCAGC TAAGCAAAAGGAGGAGTAAGAAAATAATTCAAAAGGCATTATTATTAGACTCTTAGTTTTAGCTGAAATTAACTTTGTTG CTTTACTCATCCTTGCTATATTTAATTCTCCAAACGAT ................................................................................ ..N..............................i.............................................. .........................................N.........N............................ ..................N..........................N........N......................... ...............................N................................................ ..............N...................i............................................. .........N.............................N........................................ ...............i......N......................................................... ...............................i................................................ ................................................N.....N.....................i... i...........................N............N.....................i................ ................................................................................ ...........N.......................................N..................N.......N. ....i........................................................................... .......................................N........................i............N.. .....................................N..............N........................... .........i.......................i.................................N............ .................N...............................i...N.............i............ ..................N.....N.....................................i................. .....................N.....................i..............i.....N............... ......i.........................................................i........i...... ................................................N............................... ....N........................................................N....i............. ......N......................N.......................N..N....................... ................................................................................ ............................................................i................... ..................................................N............................. ...........i...N................................................................ .....................................N................i.........N........N..N... ..................................i............................................. .........N..........................................................N........... .........N....................................................................i. ......................................N......................................... ........N........................N.....................................N........ .......N.................................N.....................N................ ...................................................N........N................... ................................................................................ ....................................N...............N..............N............ ....N...................................N....................................... ................................................................................ ................................................................................ ................................................................................ ...................................... Pos Score Pred ------------------------ 83 0.075 - 114 0.765 Yes 202 0.289 - 212 0.474 - 259 0.121 - 286 0.071 - 295 0.088 - 352 0.184 - 415 0.152 - 435 0.876 Yes 490 0.098 - 520 0.209 - 576 0.853 Yes 583 0.090 - 672 0.589 Yes 769 0.124 - 775 0.099 - 797 0.759 Yes 801 0.768 Yes 829 0.297 - 842 0.485 - 864 0.601 Yes 972 0.262 - 1012 0.440 - 1031 0.339 - 1039 0.108 - 1045 0.552 Yes 1160 0.454 - 1185 0.854 Yes 1198 0.410 - 1238 0.377 - 1253 0.287 - 1290 0.796 Yes 1314 0.711 Yes 1348 0.299 - 1378 0.122 - 1410 0.827 Yes 1414 0.181 - 1428 0.809 Yes 1459 0.152 - 1465 0.094 - 1503 0.714 Yes 1542 0.458 - 1564 0.536 Yes 1579 0.616 Yes 1585 0.338 - 1607 0.567 Yes 1665 0.668 Yes 1674 0.663 Yes 1729 0.354 - 1765 0.260 - 1822 0.131 - 1827 0.721 Yes 1847 0.254 - 1870 0.356 - 1894 0.121 - 1897 0.285 - 2061 0.830 Yes 2131 0.130 - 2172 0.561 Yes 2176 0.474 - 2278 0.188 - 2295 0.914 Yes 2305 0.171 - 2314 0.120 - 2317 0.109 - 2355 0.656 Yes 2410 0.233 - 2469 0.300 - 2490 0.437 - 2559 0.511 Yes 2599 0.240 - 2649 0.438 - 2674 0.238 - 2712 0.367 - 2728 0.449 - 2762 0.292 - 2784 0.306 - 2852 0.239 - 2861 0.404 - 2997 0.397 - 3013 0.156 - 3028 0.478 - 3045 0.117 - 3081 0.336 -

Explain the output -- Processing locus 74: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACTATAAGGAATCCTTTCTGTAAATTGATGCGAATCTTGCAAAGATTTTATTTGTTTTATCGAATTGAATCTCCTGTCT GTCTATCAAGCTTCTTAGTAGCAGCGCCAAAGATGTAAGTATTGTCTTGATCTATTGCAGGCTGTGAATGGAGAAGTGCC AAAGGAGGAGAAAGAAGAGGAAGATGGGGAATTCATAAAAGTTGAGAAGGAAGCTTTTGATGCCAAGGATGATGCGGAGA AAGCAGATCATGTTCCTGTTGAAGAGCAAAAGGAAGTTATTGAAAGAAGCTCGAGTGGTTCACAGAGAGAACTACATGAA TCACAAGAAAAGGCAAAAGAACTAGAGCTCGAATTGGAAAGAGTTGCAGGGGAACTAAAACGGTATGAATCTGAAAATAC CCATTTGAAGGATGAGCTTTTGTCTGCGAAAGAGAAGTTAGAAGAAACGGAAAAGAAGCATGGAGATCTTGAAGTTGTCC AAAAGAAGCAGCAAGAGAAAATTGTTGAAGGGGAAGAGAGACACAGCTCACAGTTAAAGTCCTTGGAAGATGCTTTGCAG TCACATGATGCGAAAGATAAGGAACTAACTGAAGTTAAGGAAGCTTTTGATGCGTTGGGCATAGAACTCGAGAGCTCACG AAAGAAGTTGATAGAGTTGGAGGAGGGGCTAAAACGTTCAGCTGAAGAGGCTCAGAAATTTGAAGAGCTGCATAAGCAAA GTGCTTCTCATGCTGACTCTGAGTCGCAGAAGGCTTTGGAATTTTCGGAGCTGCTTAAATCGACAAAAGAGAGTGCCAAG GAAATGGAGGAAAAAATGGCGTCCCTGCAACAAGAAATCAAGGAGCTCAATGAGAAGATGTCTGAAAACGAAAAGGTTGA AGCTGCCTTAAAGAGTAGTGCGGGAGAATTAGCTGCAGTGCAAGAGGAATTGGCACTTTCAAAATCTCGTTTGTTAGAGA CGGAACAAAAAGTGTCTTCCACAGAAGCCCTCATTGATGAACTGACTCAGGAACTTGAGCAGAAGAAAGCTTCCGAATCT CGGTTCAAGGAAGAGTTATCCGTTCTCCAAGATTTGGATGCTCAGACCAAAGGTCTTCAGGCTAAACTTTCTGAACAGGA AGGTATTAATTCAAAGCTTGCGGAAGAACTCAAGGAGAAGGAACTTCTGGAATCCTTATCAAAAGACCAAGAAGAGAAGC TGAGAACTGCAAATGAGAAGTTGGCTGAAGTTTTGAAAGAAAAAGAAGCGCTTGAAGCAAATGTAGCAGAGGTCACAAGT AATGTAGCAACAGTAACAGAGGTCTGCAATGAACTAGAAGAGAAACTGAAGACCTCAGATGAGAATTTCTCCAAAACAGA TGCTCTTCTTTCTCAAGCTTTGTCAAACAACTCTGAGCTTGAACAGAAACTGAAATCGCTGGAGGAGCTTCATTCTGAAG CTGGATCTGCAGCAGCAGCTGCTACCCAGAAGAACCTTGAGCTAGAGGATGTTGTTCGGTCCTCTAGTCAAGCAGCAGAA GAAGCCAAATCACAGATAAAAGAGCTGGAGACAAAGTTCACTGCAGCTGAACAAAAGAACGCGGAGCTTGAGCAGCAACT GAATTTATTGCAGCTGAAAAGTAGTGATGCAGAACGCGAGTTGAAGGAACTCTCCGAGAAATCATCTGAACTACAAACTG CTATTGAAGTTGCCGAAGAAGAGAAAAAACAGGCGACCACTCAGATGCAGGAATACAAACAAAAAGCATCAGAATTGGAA TTGTCTCTGACCCAATCATCGGCCAGAAATTCAGAGCTTGAAGAAGATTTGAGAATCGCTTTGCAGAAGGGTGCAGAACA TGAAGACCGAGCTAATACAACTCACCAGCGCAGTATTGAACTAGAAGGTCTTTGTCAATCATCACAGTCAAAACATGAAG ACGCAGAAGGAAGATTAAAAGACTTGGAGCTTCTACTCCAGACAGAAAAGTACAGAATTCAGGAACTTGAGGAGCAGGTT AGCTCGCTAGAAAAGAAGCATGGGGAAACTGAAGCAGATTCTAAAGGTTACTTGGGTCAGGTGGCTGAGCTTCAATCCAC ACTAGAGGCATTTCAAGTGAAAAGTTCAAGCCTCGAAGCTGCTTTAAACATCGCAACTGAAAACGAGAAAGAACTGACAG AAAATCTGAACGCTGTGACGAGTGAGAAAAAGAAATTAGAGGCAACAGTGGATGAGTACAGCGTGAAGATCAGTGAGTCT GAGAATCTGTTAGAAAGTATCAGGAATGAATTGAATGTCACACAAGGAAAGTTGGAGAGTATTGAAAATGATCTGAAAGC TGCTGGCTTACAGGAAAGTGAAGTAATGGAGAAACTGAAGTCTGCTGAAGAGAGTCTTGAGCAGAAAGGAAGAGAAATAG ATGAAGCTACTACGAAACGTATGGAGCTTGAAGCTTTGCATCAGTCTTTAAGTATAGACTCAGAGCATAGACTTCAAAAG GCAATGGAGGAGTTCACTAGCAGAGATTCAGAGGCCAGTTCTTTGACTGAGAAGTTGAGAGACTTGGAAGGCAAAATAAA ATCATATGAAGAGCAGTTGGCTGAAGCATCTGGCAAATCTTCTTCTTTGAAAGAAAAACTTGAACAGACTTTGGGAAGGC TTGCAGCTGCAGAATCTGTTAATGAAAAACTCAAACAAGAGTTTGATCAGGCACAAGAAAAATCTTTGCAATCATCATCA GAGAGTGAACTACTAGCAGAGACAAACAACCAACTCAAGATCAAAATTCAAGAGCTTGAGGGGTTAATAGGTTCTGGTTC TGTCGAGAAAGAAACTGCATTGAAAAGGTTAGAAGAGGCAATCGAAAGGTTTAATCAGAAAGAAACAGAATCTAGTGACT TGGTTGAAAAGTTAAAAACACATGAAAACCAAATCGAGGAGTACAAAAAGCTGGCTCATGAAGCATCAGGAGTTGCGGAC ACTAGAAAAGTTGAGCTTGAAGACGCGCTATCAAAGTTGAAGAATCTTGAATCTACTATTGAAGAGCTCGGGGCCAAATG CCAGGGGCTTGAGAAAGAAAGTGGGGATTTGGCCGAGGTGAATTTGAAGTTGAATCTGGAACTAGCCAATCATGGATCTG AGGCCAACGAGTTGCAGACAAAGCTCTCTGCCTTGGAGGCCGAGAAGGAGCAAACAGCCAATGAGCTTGAGGCTTCAAAG ACAACCATAGAAGATCTAACAAAGCAGCTTACCTCTGAAGGGGAGAAATTACAGTCACAGATTTCTTCCCACACTGAGGA GAACAACCAAGTCAATGCGATGTTTCAGAGTACCAAGGAGGAGCTGCAATCAGTTATTGCCAAGCTTGAAGAACAACTAA CCGTAGAGAGCTCTAAAGCTGATACTCTGGTCTCCGAGATTGAGAAGCTCAGGGCAGTGGCTGCTGAAAAGTCCGTTCTG GAATCACATTTTGAAGAACTTGAAAAAACTTTGTCAGAGGTCAAAGCTCAGTTGAAAGAAAATGTGGAAAATGCAGCTAC TGCGTCTGTAAAAGTGGCGGAATTGACCTCAAAACTGCAGGAACACGAACATATTGCTGGTGAACGAGATGTGCTCAACG AGCAAGTGCTTCAGCTTCAGAAAGAGCTTCAAGCGGCTCAGAGTTCCATTGATGAACAGAAACAAGCACACTCCCAGAAG CAATCAGAGCTGGAGTCTGCATTAAAGAAATCACAAGAAGAGATTGAAGCTAAGAAAAAGGCTGTCACTGAATTTGAATC AATGGTCAAAGATCTTGAACAGAAAGTGCAGCTCGCAGATGCTAAAACTAAGGAAACGGAGGCAATGGATGTAGGTGTCA AATCTCGAGACATTGACTTATCCTTTTCTTCTCCAACGAAACGTAAGAGCAAGAAGAAGCCAGAAGCATCTCTTTCTTCT TCTTCGTCTTCGGGCAATGTTACTACTCCTACTCAAACAGCATCAACTTCACATCTCATGACAGTGAAGATCGTCACTGG AGTGGCGCTGATCTCTGTTATCATCGGTATAATTCTTGGGAGAAAGTATTAGTCTCGTCTTCTCAGTGTGGCTTGATGAT GTTCATATCTGAAATGAAGATCTCTCCTTCTTCATTTGTTTTTTTTGGGGGTTTTTGAATTTTGATAGCAG ............................N................................................... ................................i..................................N............ .......................N...................................N........N..N........ .........N.................................................................N.... ................................................................N............... ...........N...............................................N.................... .....................................................................N.......... ....N..N.........................................N.............................. ................................................................................ .........N...................................................................... ...i...........i.................................N.......i...................... ................................................................................ ....................................N........................................... .....................................N.......................................... ................................................................................ ............N...............................................N................... ................................................................................ ................................................N............................... ................................................................................ ..........................N..................................................... ............................................N................................... ...............................................................................i ..........................................................................N..... ................................................................................ ...................i............................................................ ................................................................................ ...................................................N............................ .........................N........N................................N............ .........................i...................................................... N...................i........................................................... ...N............................................................................ .....N.......................................................................... .....................N.......................................................... ................................................................................ ................................................................................ .....................N...................................N...................... .............................................................................i.. .......................................................................N........ ............................................................N................... ................................................................................ ..............N....N............................................................ ................................................................................ .............................................................i........N......... ....................................................................N........... ...................................................N............................ ................................................................................ ................................................................................ ................N........................................N...................... ...........................................................................N..N. .............N......................................................... Pos Score Pred ------------------------ 29 0.152 - 113 0.546 Yes 148 0.158 - 184 0.204 - 220 0.249 - 229 0.248 - 232 0.248 - 250 0.131 - 316 0.106 - 385 0.278 - 412 0.283 - 460 0.263 - 550 0.192 - 565 0.101 - 568 0.358 - 610 0.290 - 730 0.134 - 804 0.832 Yes 816 0.851 Yes 850 0.291 - 858 0.596 Yes 997 0.118 - 1078 0.143 - 1213 0.215 - 1261 0.133 - 1282 0.173 - 1309 0.233 - 1339 0.163 - 1360 0.308 - 1489 0.159 - 1627 0.461 - 1725 0.483 - 1840 0.664 Yes 1915 0.227 - 2020 0.516 Yes 2212 0.168 - 2266 0.168 - 2275 0.263 - 2308 0.457 - 2346 0.727 Yes 2401 0.160 - 2421 0.612 Yes 2484 0.351 - 2566 0.157 - 2662 0.327 - 2902 0.099 - 2938 0.114 - 3038 0.727 Yes 3112 0.174 - 3181 0.256 - 3295 0.202 - 3300 0.460 - 3502 0.589 Yes 3511 0.201 - 3589 0.382 - 3652 0.240 - 3762 0.461 - 3799 0.194 - 3825 0.440 - 3829 0.114 - 3937 0.105 - 3978 0.302 - 4076 0.063 - 4079 0.088 - 4094 0.131 -

Explain the output -- Processing locus 75: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTACTATTTGCCTATTTCTATAGTAAGTAAATAGTATTTATTTGTCGGGTAAAGTAAACGTATTAAACATTTCCGTAT AAGAAAATGCATTTTTAATAATATTTAAGGCTTTGCTTCATGCGGTGGCTTTGCGTGGGTCCTACAGAGAATACAGCAAG CAAGAATTACTGTTTAGTTGTCTCTTTGGTTGGTTTGGTGATTAAAGAAGAAAAGAACCCAAAGTTTTGCAATTGCATTG ACCTTACAGCTCACAGAGACTCTCAGACTTGGCTTTCCTATCAAAGCTCTTTTACATTTGCATTCAAGCTTAAGCAACGT CAATTCCAACTACAACAACCGCATTACTTTCACTTGCAAACAAAACACGAATCCAAAACTTCCATTTTTGTTCACAAATC TCAGCTTCTTCTGATTCAAAAAGTTTTGAGCTTTATTGATCCAATGTTGGTTCAGGATCGTGCGGCGCCTTCTCCTGCCA AGCCGCCGAAATCTCAGATCAGAGAACTACCTACTCATCAACAGATCCGACGACGATTCAGTGAACCCAAAAACTTAGAT TTCTCGACTTGGTTCTCTGAGAATCTCTCTAGAATTGCTGTCTTCTCTCTCTTAATCGTCACCATAGTAGCTTTCTTCTT CCTCTACAACACCACCGACACTGCTTCTCTCCTTTGTTTCCAGTCTCAGTCAACTCAATTTCTTCAATCTCTCTCTCGCC CTCAAATCAAATGGAATTCGATCCCAGTCGTCCCTGATAAAACCTCCCCGTACGCTAATTTCCAGACAGAGAAATGGATT GTTGTGTCGGTTACCAAGTACCCAACTGAGGAGCTCAAGAGCTTGGTGAAGATTCGTGGATGGCAGGTTCTAGCTATTGG AAACTCGGCGACACCCAAGGATTGGAGTCTTAAAGGTTCCATCTTTTTGTCTCTCGATGCTCAAGCTGAATTGGGTTATC GAGTTTTAGATCACTTGCCTTATGATTCTTTCGTAAGGAAGAGTGTTGGTTACTTGTTCGCGATCCAGCACGGTGCGAAG AAGATCTATGATGCTGATGATCGTGGTGAAGTGATTGATGGAGATCTAGGCAAGCATTTTGATGTTGAGTTAGTTGGTCT AGATTCTAAGCAAGAACCGATTTTGCAGTATAGTCATGAGAACCCCAATAGGACTGTGGTTAATCCTTATATTCATTTCG GGCAACGTTCCGTTTGGCCTAGAGGTTTGCCATTAGAGAATGTAGGTGAGATCAATCATGAGGAGTATTACACTGAGGTT TTTGGTGGTAAGCAGTTTATACAGCAAGGGATTTCTAACGGTTTACCGGATGTTGATTCGGTGTTTTACTTCACTAGGAA GACGACTTTAGAAGCTTTTGATATTAGGTTCGATGAGCATTCTCCTAAAGTTGCTTTACCTCAAGGTGTGATGGTGCCGG TTAATTCGTTTAACACTCTTTACCATTCGTCAGCCTTTTGGGGGTTAATGCTTCCTGTTTCGGTTAGTTCCATGGCTTCT GATGTGCTGAGAGGTTACTGGGGACAAAGGCTTTTGTGGGAGCTTGGTGGTTATGTTGCTGTTTATCCACCTACAGCTCA CCGGTTTGATAGAATCGAGGCTTACCCTTTTGTAGAGGAGAAGGATCTTCATGTTAATGTTGGTCGTTTGATCAAGTTCT TACTTGCTTGGAGATCAGAGAAGCATAGTTTCTTTGAGACGGTCCTTGATTTGAGCTTTGCTATGGCTGAAGAAGGGTTT TGGACAGAACAGGATTTGAAATTCACAGCTGCTTGGCTTCAGGATTTGATTGCTGTTGGGTACCAACAACCGAGATTGAT GTCGCTGGAATTGGATCGCCCGCGAGCAAGTATCGGTCATGGAGATAGAAAAGAGTTTGTTCCAAGGAAGTTACCTTCGG TTCATCTCGGGGTTGAGGAGACTGGCACAGTGAGCACTGAAATAGGGAACTTGATTAGGTGGAGGAAGAATTTTGGGAAT GTTGTGCTGGTTATGTTTTGTAACGGTCCCGTTGAACGTACCGCACTCGAGTGGAGGTTACTTTATGGCCGGATATTCAA AACTGTTGTGATACTTTCTTCTCAGAAGAACTCAGATCTCTATGTAGAAGAAGCCAAACTCGATCACATTTACAAGCATC TACCAAAGATATTTGATCGATACAGCAGCGCAGAAGGATTTTTGTTTGTTGAAGATGATACAGTTCTCAATTACTGGAAT CTACTTCAAGCTGACAAATCTAAGATTTGGACTACAGACAAGGTATCAAAGTCTTGGACATCAGTGAAACCGACCGGGAA CTCTGATTGGTTTTCTGTACAAGCAGAGTTAGTGAAGAAAACTGTAAGTACAATGCCAGCTCATTTCCAAGTTAACTACA AGGACGCCACCAAGAACAATCATGAGACCTTAACAGTATGCAGTTCAGAGGTATTCTATGTGCCTAAAAGACTGGTAACT GACTTTATCGATCTTGTTGATCTCGTGGGCGATATGGACTTGCATTACAAAGTGGCTGTGCCAATGTTCTTCTTGTCTAT GGATTCGCCTCAGAATTTTGACCCGGTTCTCGGTTCAATGGTATATAAGAGGAAATCAGCTTCGTTTAACACTTCTTCGA GTCTGTATTCTGCTAAAGCACCTGCGGTTCACCCGTGGAGCATATCAAGCGAACAAGATTTCATCAAATTGGTTCAGCAA ATGGCTGAGGGCGACCCGCTTCTGATGGAGTTGGTATGATGGACTCAGGGTATAGAGCTTGCAGTAATGCAATGATAGAA ACAGAGTTATACAAAAATTGTGTTTTTGATTCTTTTCTTATTGGTTCCCTTTTCAGAA ................................................................................ ......N................................N........................................ ................................................................................ ................................................................................ ................................................................................ ...........................................N.................................... ................................................................................ ................................................................................ ................................................................................ ..........i..............................................................i...... ...........................................................N.................... ........................................................N....................... .....................N.......................................................... .......N..N.....N....................N.......................N.................. ...................................N............................................ .......................................N.................N...................... .................................................N.............................. ................................N.....................................N......... ...............................................N.......................i........ ..................................................N.....N....................... ..............................................................i................. ..............................................................................i. ......................................N......................................... ..............................................................................N. ............N...................................................N............... .........................................N...................................... ......................................................N......................... ................................................................................ ....................................................i........................... .....................N...............N...................N...................... .................................i.............................N..............N. .....................................i.......................................... ................................................................................ i.......................N..........N..N...........................N....N........ .......................................................... Pos Score Pred ------------------------ 87 0.275 - 120 0.487 - 444 0.431 - 731 0.746 Yes 794 0.793 Yes 860 0.305 - 937 0.233 - 982 0.106 - 1048 0.186 - 1051 0.203 - 1057 0.119 - 1078 0.134 - 1102 0.078 - 1156 0.235 - 1240 0.245 - 1258 0.384 - 1330 0.109 - 1393 0.178 - 1431 0.402 - 1488 0.384 - 1512 0.691 Yes 1522 0.210 - 1573 0.184 - 1651 0.114 - 1657 0.365 - 1743 0.637 Yes 1839 0.805 Yes 1879 0.307 - 1999 0.245 - 2013 0.255 - 2065 0.335 - 2122 0.307 - 2215 0.406 - 2373 0.518 Yes 2422 0.107 - 2438 0.380 - 2458 0.233 - 2514 0.668 Yes 2544 0.294 - 2559 0.326 - 2598 0.605 Yes 2721 0.744 Yes 2745 0.283 - 2756 0.476 - 2759 0.395 - 2787 0.053 - 2792 0.079 -

Explain the output -- Processing locus 76: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTTCTTGTCGGTGTTTTCTTATCACATATTTACCAAAAATCTACCCACCTTATCTTAACTTAATATACTTCCACTTATA TAATCTAATGTATGCGTTTAAGAAATGAGGTACGTAGCTCACATTTTTGTAATGTGGAGGTTGAAGATAGGAGAAGGGAA CGGAGATGATCCCTACTTATTCACTACTAACAACTTCGCCGGACGACAAACATGGGAGTTTGATCCTGACGGAGGCTCGC CGGAGGAAAGACACTCCGTCGTTGAGGCTCGCCGGATTTTCTACGACAATCGTTTTCATGTTAAAGCAAGCAGCGATCTC TTGTGGCGCATGCAGTTTCTGAGGGAGAAAAAGTTTGAGCAACGGATAGCTCCGGTGAAAGTTGAAGACTCCGAGAAGGT AACCTTTGAAACGGCGACGAGTGCATTACGGAGAGGTATTCATTTCTTCTCGGCGTTGCAGGCCAGCGACGGTCACTGGC CGGCGGAAAATGCCGGCCCTTTGTTTTTCCTTCCGCCACTGGTGTTTTGTCTTTACATAACGGGACATCTTGATGAAGTG TTCACTTCAGAACATCGAAAAGAGATTCTTCGATACATCTACTGTCACCAGAAGGAAGATGGTGGGTGGGGATTACACAT TGAAGGTCATAGCACAATGTTCTGTACCACGTTGAATTACATATGTATGCGTATACTTGGAGAAAGTCCTGATGGAGGTC ATGATAATGCGTGTGGACGAGCCCGGGAATGGATCCTTAGTCATGGTGGTGTCACCTACATACCTTCTTGGGGTAAAACT TGGCTTTCGATACTTGGTGTATTTGACTGGTCTGGAAGCAACCCAATGCCTCCTGAGTTTTGGATCCTACCTTCTTTCTT TCCCGTGCATCCAGCAAAAATGTGGAGCTACTGCCGGATGGTTTACTTGCCAATGTCGTATCTCTACGGGAAGCGGTTTG TTGGCCCAATAACGTCTCTGATTTTGCAACTACGCAAAGAACTGTACTTACAGCCTTATGAAGAAATCAATTGGATGAAA GTCCGCCATCTTTGTGCAAAGGAAGATACATATTATCCCCGTCCACTGGTTCAAGAGTTGGTATGGGACAGTCTTTACAT CTTTGCGGAGCCTTTCCTTGCACGTTGGCCATTTAACAAGCTTCTTAGGGAAAAGGCTCTTCAATTGGCAATGAAACACA TACACTATGAAGATGAAAATAGTCGTTATATCACCATTGGGTGTGTTGAAAAGGTACTATGCATGCTAGCTTGTTGGGTT GAAGACCCGAATGGGGATTATTTCAAGAAGCACCTCTCTAGAATCTCCGATTACTTGTGGATGGCTGAAGATGGGATGAA AATGCAGAGCTTTGGAAGTCAACTATGGGATACAGGGTTTGCGATGCAAGCTTTACTTGCAAGCAATCTCTCCAGTGAAA TCTCTGATGTACTCAGGAGAGGACATGAGTTCATAAAGAATTCACAGGTTGGAGAGAACCCTTCAGGTGATTACAAAAGC ATGTACCGTCATATTTCAAAAGGAGCATGGACTTTTTCTGACCGAGATCACGGATGGCAAGTTTCAGACTGCACAGCTCA TGGCTTAAAGTGCTGCCTGCTGTTCTCGATGTTGGCGCCGGATATTGTTGGCCCAAAACAAGACCCCGAGAGACTACATG ATTCTGTTAATATCTTGCTCTCTTTACAGAGCAAAAACGGAGGTATGACTGCCTGGGAGCCTGCTGGTGCTCCCAAGTGG TTGGAATTGCTCAATCCCACAGAAATGTTTTCCGACATTGTGATTGAGCATGAGTACAGTGAATGTACATCGTCTGCAAT CCAAGCATTGAGTCTTTTCAAGCAACTCTACCCTGATCACAGGACAACAGAGATCACCGCTTTCATCAAGAAAGCTGCGG AATACCTAGAAAACATGCAAACACGTGATGGTTCATGGTATGGGAACTGGGGCATTTGCTTCACGTACGGTACATGGTTT GCTCTTGCAGGCTTAGCAGCTGCGGGTAAGACTTTTAACGACTGTGAGGCAATACGCAAAGGCGTTCAGTTTCTTCTTGC AGCTCAGAAAGACAACGGAGGCTGGGGAGAAAGCTACCTCTCTTGCTCCAAAAAGATATACATAGCACAAGTAGGGGAGA TATCAAACGTGGTGCAAACTGCTTGGGCTTTAATGGGTCTCATTCACTCTGGACAGGCAGAGAGAGATCCGATTCCTCTT CACCGTGCTGCGAAACTTATCATCAATTCACAACTGGAGAGTGGAGATTTTCCTCAACAGCAAGCAACCGGAGTGTTTTT GAAGAATTGCACATTACACTACGCTGCATATAGAAACATTCATCCGTTGTGGGCACTCGCAGAATATCGCGCGCGAGTTT CGTTGCCATGACGACACTTTTCATAAACTTTTCTTTATATCAGAGAACATGTGAATTTATTAACCTATCATCAGTCTATT ACAAGAATTCGTGTATGCGATATGTCAATG ................................................................................ .......i...N............i..........................i............................ .....i.............................................i............................ .........................................................N...................... .........N...................................................................... ................................................................................ .........N..............................................................i....... ..........................................................i..................... ................i.........................N...N........................i........ N.....N.....................N.............N..................................... .............................................N.................................. ...................i.................N..............N........................... .........................................................N................N..... ..............................................................i................. ......................................................................N......... ......i.....N.............................................i...N................. ..........N.................................................i.........N....N.... ......N.................N....................................................... N.........................i..........................i.........................N ............................i................................................N.. ............................................i................................... ........................N........................N............i................. ................................................................................ ..............N............i......N....N.................................N...... ................................................................................ ................................................................................ ................................N............................................... ................................................................................ ................................................................................ .......N........................................N............................... ..............N......N.....N.. Pos Score Pred ------------------------ 88 0.539 Yes 92 0.396 - 105 0.843 Yes 132 0.703 Yes 166 0.771 Yes 212 0.693 Yes 298 0.234 - 330 0.387 - 490 0.326 - 553 0.642 Yes 619 0.618 Yes 657 0.660 Yes 683 0.190 - 687 0.443 - 712 0.711 Yes 721 0.134 - 727 0.474 - 749 0.495 - 763 0.310 - 846 0.466 - 900 0.659 Yes 918 0.178 - 933 0.269 - 1018 0.236 - 1035 0.457 - 1103 0.502 Yes 1191 0.496 - 1207 0.522 Yes 1213 0.384 - 1259 0.788 Yes 1263 0.444 - 1291 0.456 - 1341 0.729 Yes 1351 0.205 - 1356 0.445 - 1362 0.387 - 1385 0.534 Yes 1404 0.514 Yes 1447 0.491 - 1465 0.441 - 1521 0.398 - 1547 0.540 Yes 1574 0.625 Yes 1600 0.122 - 1629 0.505 Yes 1678 0.421 - 1725 0.708 Yes 1785 0.291 - 1810 0.298 - 1823 0.549 Yes 1935 0.399 - 1948 0.515 Yes 1955 0.293 - 1960 0.465 - 1994 0.429 - 2193 0.457 - 2408 0.291 - 2449 0.148 - 2495 0.039 - 2502 0.062 - 2508 0.043 -

Explain the output -- Processing locus 77: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGACACCTTTCTGGTTTAAGACAAAGTCAAATACTCAACCTTGAATGTTTCCTCTAAATATTTCTGAGTAAATAAAGAC CAATACTTAATTTATAGGTGAACAATGTGCTCTTGGCACGCACATGGACATGGCATGATGGCACGACGAGGATTAAGGAT GGACAACTCTGAAGCACATCTAGATAGAATTGAGGCACAGAGGTCAACAGATGACCAACATAAACGAAGATGCTTCCCTG TGAATGTGATAAAAAATCTAGGCACACTCAGTTCACGCAGTTTCCTTCGACGCAGAGGAGATGAAGCCACACCACCTACG ACTACGATAGACATTGAATCTGTGCCAAAGTTCCTTACCACTCTCAGATTGTTGGATCTTTACAACCACCCCGGTGAATA TGTGGCAATGAATTCAGAGATCTCCAGCGCAATGCGAGCTGGGAACAAAGAATTTCTGGAGAAGATGGAAAGCTACGAAA CACCAATGTCATGCTTCAAGAACAACAAAGGAGATTCCATTCTTCACCTTGCTGCTGCTTTTGGTCATCTTGAGCTAGTA AAGAGCATAGTCTCTAAATTCCCATCTCTTCTGTTGGAGTTAAATTTTAAGGATCAGCTTCCGCTTCATGTGGCTGCTCG TGATGGTCATTTAACAGTTGTTAAAGCTCTTGTAGCATCGGTAACATTTTGTTCAGATAGACTGGCTGAAGAAGATAGGG AGAGGCTGAATCCATATATTCTAAAGGACAAAAATGGAGATACTGCTCTCCACTCAGCCTTGAAAGACCTCCATGAAAAA ACAAAAGAACTCCATGAAAAAACAAAGGATATGCATTGGCTACGACGTAGCAAATCCAAGTCACTTTCTAATGAATCTAC ACACCTAATGGAGACAGCTGCTTGTCTAGTCAATGCGAACCAAGATGTTTCGTTTCTTGCAAATAAAGATGAAATCTCCC CCTTGTATTTGGCAGTTGAAGCTGGTAACGTATCACTTGTAAATGCAATGTTGAACAGCCATGTTAATAACGTTCAAGAC AAAACCTTTAACTTAGCCACACAATTGAAAGGGAGAAAATCGCTTGTGCATGCAGCTTTAAAGGCAAAGAATACAGATGT TCTTGATGTTATTCTCGGTAAATACCCGAGTCTTGTCAAAGAGCGAGACGAAAAAGGACGAACTTGTCTTTCGGTTGGAG CATCTGTAGGGTTTTATCAAGGCATATGCAAGCTGTTAGACACATCAACGCTAAGCATCTTTGATTGCGACGATGATGGT TCATTTCCCATCCATAAGGCAGTGGAGAAAGGTCATGAGAATGTTGTTAAAGAGCTTCTAAAACGTTTTCCGGATTCAGT AGAGCAGCTTAACAAAGAAGGTCAGAACATTTTCCACATATCAGCAAAGAGTGGCAAATCCACATTGTTTCTTATGGAAC ATATCAATAAAGTCGATACGAAGAATCATCTTATGGAGGAGCAAGATATGGATGGGAATACACCTTTGCACCTGGCCACC ATAAATTGGCGCCCTAAAACTGTTCGTATGCTTACTAAATTTCTTTCCATCAGAAAAAAACTCTTGGATAAACATAACTC CGTTGGCTTGAGACCTCTGGATATCGCAGAGATAAATCTGCAGTCTGACTACGTTTTTCGAGAGAGAATGACTTTGATGG TCTTGCTAGGCGTTTACAATCTAAGACAGAGAGGTATTTCTTTGCTACCGACTAGCGGGATGACACTAAGAAGTAGATCA GAAAAACTAGGTGATGGTGAAAAATACAAAGACCGTGTCAACATTCTTCTACTGGTGGCAGCTCTTGTAGCCACTATGAC GTTTGCTGCAGGTTTTACAATGCCCGGTGGCTTTAGCAGCTCTGCTCCAAACACTGGCATGGCTATTTTGGTCGATGACC GTTACCTCACAACATTTATTATGAATGACACTATTGCAATGCTTACCTCAGTTTTAGCAATAGTTGCTCTTATCTGGGCA CAGTTAGGAGATCCAGAACTCGCGCACAGAGCGTTTCATTTGGCTTTACCAGCACTCTTCGTTGCTCTACTCTTCATGTG TTTCACATTCTTTTATGGCGTTCTGGCTACAATACAGCATAACATCGTGCTTTCACGCATCATAAGCTTTGTATTTATTA TCTTATTTATTATGACCGGAACCCTCCTTACCCCTTACGTGATCCCGCAGGTTTACGGCCTTCCTATTATTGGAGTTTTG ACTAGTTCATATCTTGATTTTCTATTGCTGTTTGTTAATGAAGATGATGATGAAAAACATTACACCACCGGCTCATCTCA CACGTGTGTGAAGAGTGGGGAGTCCATGGGATCTTCAAGCGGGGTCCAAGCGGAAAAGTCGAAAGTGCTTTAAAGAGATT GCATCGTTTCAAATTAGCTTTCCTTATTTGGGTCACTAAAGTCTTGGCATTTACTTTCCTGCTTGTAATAAACGCGGCTA CTGTTAATGTTT .............................................i.................................. ........................i..................N.....N....N..i....................i. ..................................................i..................i.......... ...N........................................................N................... ...............................................................................N .......i.......................N................................i............... .....N....N..................................................................... ...................................................................N............ ..N............................................................................. .................................i......................................N....... .............N................i.......................................N......... .......i........................N...........N.......................N........... ..........................................N....i............N................... .................................................N..........................N... .....N.......................................................................... .........................N..............................................N..N.... ..................................N.....N....................................... .........................................................................i...... ................................i..............i...N............................ ...........................i.................................................... ...................................................................N........N... ...........................................................N.................... .............N.............................................................i.... ...................i......................................i...............N..... ....................i...N.............N......................................... ...........................................................................N.... ..............N................................................................. ...........N.................................................................... .....................................N.....N..i..N.............................. .........................N...................................................... ................................................................................ ......N..... Pos Score Pred ------------------------ 46 0.599 Yes 105 0.665 Yes 124 0.491 - 130 0.431 - 135 0.389 - 138 0.649 Yes 159 0.517 Yes 211 0.582 Yes 230 0.686 Yes 244 0.235 - 301 0.423 - 400 0.384 - 408 0.665 Yes 432 0.240 - 465 0.684 Yes 486 0.377 - 491 0.146 - 628 0.301 - 643 0.133 - 754 0.701 Yes 793 0.163 - 814 0.242 - 831 0.605 Yes 871 0.174 - 888 0.706 Yes 913 0.138 - 925 0.123 - 949 0.209 - 1003 0.232 - 1008 0.579 Yes 1021 0.078 - 1090 0.109 - 1117 0.382 - 1126 0.306 - 1226 0.443 - 1273 0.202 - 1276 0.298 - 1315 0.230 - 1321 0.380 - 1434 0.795 Yes 1473 0.628 Yes 1488 0.625 Yes 1492 0.361 - 1548 0.546 Yes 1668 0.387 - 1677 0.226 - 1740 0.427 - 1774 0.319 - 1836 0.585 Yes 1860 0.781 Yes 1899 0.748 Yes 1915 0.159 - 1941 0.651 Yes 1945 0.247 - 1959 0.327 - 2076 0.151 - 2095 0.120 - 2172 0.259 - 2278 0.366 - 2284 0.376 - 2287 0.620 Yes 2290 0.411 - 2346 0.323 - 2487 0.038 -

Explain the output -- Processing locus 78: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGTTTTGAGGTATTTATTATTATTTCTTGATCCCACATTTGTTTCAATCGCTTTAAGCTCCAAATTTGTAATTTCAGTG TCATCTTCGTACTTGTACTGAAATGCATTGAAAATGGCAGAAGCGATGGAGACGGAGATGGATTTCTCAGATGGTGAACA AACTAATGGTAATTCACATGTTACTGCCTCCCAATACTTTGCTCCTCCGGGTTATAATAGAAGTCTTGTGGCTGCTTATG GGAATGGAAACACAACCATTGGATTAGAAAAAGGAATAGAAAGGAGACTAGATCATCATGAGCAACTACCTGGTTATATC TTTATGTGTAATGGGAGAACGAAAACCGATTGCTACCGTTACCGTGTTTTCGGGATTCCAAGAGGAGGAAAAGACGTTGT TGAGAGTATAAAGCCAGGCATGAAGCTTTTCCTCTATGACTTTGAGAAGAGACTTCTTTATGGTGTGTATGAAGCTACTG TTGGTGGCAGGTTGGATATAGAACCTGAAGCTTTTGAAGGGAAATATCCAGCTCAAGTTGGATTTAGAATTGTCATGAAC TGTTTACCCTTGACAGAAAACACTTTCAAGTCTGCTATCTATGAAAATTACAAAGGTTCCAAATTTAAGCAAGAACTTTC TCCTCACCAAGTTATGTCTCTGTTATCATTGTTTCGTTCATTTACGTCTCCAGAACTCGATTTGTTGCCTCATAGGTTGG CTTCCAGAGCTTCAGCACCTAGGACTCTATCGTTTGAGGAAAGGTTCATAGCGGCTACACATCTTAGAAATGCTTCATCG GTTTTAGATCCTCTGTCTGCTCGTCATGTAGAACCACGACTTGGCTCTGTTATGGCCCATCAGCCTGTTCCCCGCACATC TCTGCTTCAACATAGTTACTTTAGACAAGACGACTACACGACCCCACCTAGAGAAAGTCTGTCAAATTTGAATCAGCCTT ATTATCCTACAGAAGCTCGGCAACTACGGTTGCTCGGAGACCCTTCTCGCTCAGATTCTCCTCGGTCCGAACCTCCGCGG TCATCTATTCAGGATCCTCAACTCAAATACCTCACCATTTTATCTAATATTCGGAGATATGGATCTGCATCAGATCGCTT GGCCTCAGAAAATGAATATCATCCGGCAACACCATCAGAGAAGGACCAGTTTGCTGTACCTTATTCTGATAATAAAAATT ACCCTTCGACTTTATCTGGGAGTGAGCATCCTTCTGCTTCTGCAGCTAATGGGAGTGTGTATAGAAGTGAGTTCTATAAT TCAGCATCACAAAAGGAAGGGGAAGCTAGTCAGCAGCATGAGATCCCTGCTGGGACTTACCATCACCCAGAAGCATCAAC AGTTTCCAATACTACAAAGTCCATGCAACCAGATATGCAGGCAGTTAGTGTTGCTCAATCTCATACCGAGACAGCTGGCT ATCCTACACCGGCACATGGTGAAGCTTCACAACCACCTGCTGGAGCAATTGGTTACACACACCAACCTCAAAGTGTAGCG GGTAATTATAGCACGCATTCACAGCCTGGGAATGTGGAAGAAAGTACACAATCATATGCTGGAACAGATAGTTACTCACA ACAGCAATATTATGCAGCTATGGGGCCTACTACTCAACTCCATGCTGGTGGATATATTCAAAAACCTCATGAAATTGGGT ACTCTCAGCAACCTCATGATGCGGCAACCGGGTACTCTCAGCAACCTCATGATGCAGCAACCGGGTACTCTCAGCAACCT CATGATGCAGCAACCGGGTACTCTCAGCAACCTCATGCCGCATCAACCGGGTACTCTCAGCAAACCTATGCTGCAGCAAC CGGGTACACTCAGCAACCCCATGCGGCGGCAGCCGGGTACACTCAGCAACCCCATGCGGCGGCAACCGGGTATTCTCAGC AACCCCATGCTGCAGCTACCGCGCACGCTCAGCAACCCTATGCTGCAGCGACCGCGCACGCTCAGCAACTCCATGCTGTA GCTACTGGGTATGCTCTGCAACTCCATGCTGCAGCTACCGGGTACGCTCAGCAACCCCATGCTGCCGCAACTGGGTATGC TTTGCAACCACATGCTCAAGCTGTTGAATACACAATGCAACCACATGCTCAAGCTGTTGGCTACATGCCGCAATACCATG CTCACGCTGTTGTATACAGTCAACAAGGTGTGACGCAAGGTTCTGTACCAAGGGCTCCTGGGACAACTGATTGTAATGCT GCAAACCAGGCATATTCTGCAACAGGAGATTGGAATGCAGTGCATCAGTCTTATTACCCTCAGACAGCAGATGCAACCAC AACGTATTATCAAACATCTTAAAACGTTAAAGCAAAGTGTTGCTACTCTGGTATGCCAAAGAGGATTCTGTTAAGTCTCG TGTATTTAAAGCATTGTTTCGGTTCAGGTGAGAAACGATTT ................................................................................ ......................N..........i...........i...........i............i......... .....N...........N...........................................................N.. ...N.....................................................N...................... ...N......N..................................................................... ...................N...............N.......................N........N........... ..........................................................................N..... ........................................N....................................... .............N.................................................................. .....................................................................N.......... .........................N.........................N............................ ................................................................................ ................................................................................ ..........................................................N..................... ...........N.................................................................... ................................................i............................... .....................................N.......................................... ......................i...........i............................................. ...............N................................................................ ...............................N.......................N........................ ...........N.......i.....................N..........................N........... ...............N..i.............................N..N............................ ....................N................................N.......................... ......N................................N................................N....... ..........N..............N................................N.................N... ...........N......................i.........N...................i............N.. ...........................................................................N.... ..................................N...................................N......... ....................................................N........................... ......................................... Pos Score Pred ------------------------ 103 0.159 - 114 0.836 Yes 126 0.636 Yes 138 0.704 Yes 151 0.547 Yes 166 0.441 - 178 0.216 - 238 0.293 - 244 0.207 - 298 0.449 - 324 0.295 - 331 0.382 - 420 0.441 - 436 0.257 - 460 0.178 - 469 0.097 - 555 0.188 - 601 0.126 - 654 0.193 - 790 0.428 - 826 0.406 - 852 0.412 - 1099 0.394 - 1132 0.388 - 1249 0.537 Yes 1318 0.138 - 1383 0.663 Yes 1395 0.781 Yes 1456 0.202 - 1552 0.360 - 1576 0.180 - 1612 0.192 - 1620 0.823 Yes 1642 0.216 - 1669 0.348 - 1696 0.234 - 1699 0.587 Yes 1729 0.197 - 1732 0.448 - 1762 0.421 - 1765 0.557 Yes 1795 0.577 Yes 1828 0.514 Yes 1861 0.367 - 1894 0.402 - 1927 0.216 - 1960 0.195 - 1993 0.295 - 2011 0.190 - 2026 0.335 - 2059 0.229 - 2077 0.340 - 2092 0.151 - 2115 0.826 Yes 2125 0.337 - 2145 0.677 Yes 2158 0.214 - 2236 0.274 - 2275 0.467 - 2311 0.349 - 2373 0.096 -

Explain the output -- Processing locus 79: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 80: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCTTTTTCTGAAAACATGTAATTGGGCCATCATGGTTCGATTCTCAGCCCAATTAAAACCGGATCATTGAGACTCACAAT AACGTCGTCGTTTAGCTCTGAGCTCTGAGCTCTTCTCTCAACTCCGACTCATGGAGGATGAACCAAACTACTGTAAAAGT TTTCAAGTTTCAATTTTTGTTCTCAATCGAAATCAAATTGAGTCTCTCTCAACCACAAAAAATGATTGTTCACCGTATAA TACCTTCCCGCGTAAAAGACCCTCTCACTCGATTTAAACCTCTCAAAAACTTGACAACTTCTTCATCTCCCGTATTCGAA CCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCGGCTTCGTTTTCTGTTTCAGATTCTTTCTTGGTGGAGAAGATTTGTTT TAGTTTGAAGCAAGGTAACAATAATGTGAGAAACCACCTGATTCGGTTAAACCCTTTAGCCGTCGTTGAGGTTTTATACC GCTGCCGCAATGATTTAACTTTAGGTCAAAGATTCGTGGATCAATTAGGTTTCCATTTTCCGAATTTCAAGCATACGTCT TTGTCTTTAAGCGCTATGATTCATATTCTCGTGAGGAGTGGAAGATTATCAGATGCACAGAGTTGTCTTCTTAGGATGAT TAGGAGGAGTGGTGTTTCTAGACTAGAGATTGTGAATTCCTTGGATTCTACGTTTAGTAATTGTGGTTCCAATGATTCGG TTTTTGATTTGTTGATTAGGACTTATGTTCAGGCTAGGAAACTTAGAGAAGCACATGAGGCTTTTACATTGCTGAGAAGC AAAGGTTTTACGGTTTCTATCGATGCGTGTAACGCTCTTATCGGGAGCTTGGTGAGGATCGGGTGGGTTGAACTGGCTTG GGGTGTTTATCAGGAGATTTCTCGGAGTGGTGTTGGTATTAATGTCTATACTCTCAATATCATGGTTAATGCTTTGTGCA AAGATGGTAAAATGGAAAAAGTCGGAACCTTTTTATCACAAGTGCAAGAGAAAGGGGTTTATCCGGATATTGTAACATAT AATACATTGATTAGTGCCTACTCTAGTAAAGGTCTCATGGAAGAGGCATTTGAGTTGATGAATGCTATGCCGGGCAAAGG GTTTAGTCCTGGTGTTTATACTTATAATACTGTGATAAATGGACTGTGTAAGCATGGGAAATATGAGAGAGCCAAGGAAG TTTTCGCGGAGATGTTAAGATCCGGGTTGAGTCCTGACTCCACTACCTACAGATCGTTGCTGATGGAGGCTTGTAAGAAG GGAGATGTGGTTGAAACGGAAAAGGTTTTCAGTGATATGAGATCTAGAGATGTTGTTCCTGATTTGGTTTGCTTTAGCTC GATGATGAGTCTTTTTACTCGGAGTGGAAATCTCGATAAGGCATTAATGTATTTTAACAGTGTGAAGGAGGCTGGTCTGA TTCCTGACAATGTGATCTATACCATTCTTATCCAAGGGTATTGTAGAAAAGGTATGATCTCTGTAGCTATGAATTTGAGA AATGAAATGCTTCAGCAGGGTTGTGCTATGGATGTTGTTACCTACAACACCATTTTGCATGGCTTATGCAAACGGAAAAT GTTGGGTGAAGCGGATAAATTGTTCAACGAAATGACTGAAAGGGCTTTGTTTCCAGATTCTTATACTTTAACTATACTGA TTGATGGACATTGTAAGCTCGGGAATCTGCAGAACGCAATGGAGCTCTTTCAAAAGATGAAGGAAAAGAGAATCAGACTC GATGTTGTGACATACAACACTCTGTTAGATGGGTTTGGTAAAGTAGGCGATATCGATACAGCAAAAGAGATATGGGCAGA TATGGTATCAAAAGAGATACTTCCCACTCCAATATCTTACAGCATTTTGGTTAACGCGTTGTGCAGTAAAGGCCATCTGG CTGAGGCATTTCGAGTTTGGGATGAAATGATCAGTAAAAATATAAAGCCAACGGTAATGATTTGTAACTCTATGATAAAG GGATATTGTCGTTCTGGTAATGCCTCAGATGGAGAAAGCTTTTTAGAGAAGATGATCTCGGAAGGTTTTGTGCCGGATTG CATCAGTTACAACACATTAATATATGGTTTTGTTAGAGAAGAAAACATGAGCAAAGCTTTTGGTTTGGTAAAGAAGATGG AGGAGGAGCAAGGAGGATTAGTACCTGATGTATTTACATATAATTCGATCTTACATGGGTTTTGTAGACAAAATCAGATG AAAGAAGCCGAGGTTGTGCTAAGGAAGATGATCGAGAGAGGTGTCAATCCCGATAGGTCAACGTATACTTGTATGATCAA TGGATTTGTTTCTCAAGATAACTTAACCGAGGCATTTCGGATTCACGATGAGATGCTGCAAAGAGGATTCTCTCCTGATG ATAAATTCTGAATCAGTATATTTTGGATTCTTGCTCAAATTCTACTTGTGATAGCTTCAAGCTGATATCCTAGATTGAGT TGATATTGGTGTTTTTTGGTCAAAGGACCA ...............N...............N................................................ ..................................................i......N...................... .............................................................i.................. ................................................................................ ................................................................................ .......................i........................................................ .........N...................................................................... ...............N....................................N......................N.... .......................................................................N........ ........................N.............................N......................... ......................N......................................................... .........................................N...................i......N........... ...N.......i.................................................................... ....................................i....................i...N....i............. ......................................N..............N........N................. ...........i..................................................i................. ....N...............................N............N.............................. .........N...........................................i..............i........... ...............................N................................................ ...N..................................i.................i....................... .....................N....N.............................N..............N........ ...................N........N......................i............................ .......................N......................i.............................i... ...........................N..........................N......................N.. ...........................N............................................N......N ...............................................N....N........................N.. ................................................................................ .............................. Pos Score Pred ------------------------ 16 0.471 - 32 0.186 - 131 0.568 Yes 138 0.261 - 222 0.782 Yes 424 0.573 Yes 490 0.118 - 576 0.190 - 613 0.436 - 636 0.281 - 712 0.073 - 745 0.168 - 775 0.222 - 823 0.134 - 922 0.112 - 942 0.512 Yes 949 0.128 - 964 0.139 - 972 0.790 Yes 1077 0.763 Yes 1098 0.693 Yes 1102 0.146 - 1107 0.798 Yes 1159 0.224 - 1174 0.408 - 1183 0.397 - 1212 0.615 Yes 1263 0.504 Yes 1285 0.261 - 1317 0.321 - 1330 0.202 - 1362 0.161 - 1365 0.539 Yes 1407 0.579 Yes 1450 0.206 - 1494 0.514 Yes 1509 0.516 Yes 1522 0.314 - 1527 0.658 Yes 1548 0.683 Yes 1552 0.143 - 1579 0.157 - 1586 0.269 - 1599 0.678 Yes 1632 0.408 - 1684 0.187 - 1719 0.748 Yes 1737 0.798 Yes 1762 0.241 - 1789 0.220 - 1832 0.259 - 1842 0.501 Yes 1942 0.105 - 1947 0.480 - 1977 0.407 - 1992 0.166 - 2020 0.335 - 2029 0.251 - 2052 0.594 Yes 2104 0.104 - 2127 0.608 Yes 2157 0.502 Yes 2188 0.175 - 2215 0.218 - 2238 0.396 - 2268 0.385 - 2313 0.141 - 2320 0.151 - 2368 0.172 - 2373 0.244 - 2398 0.185 -

Explain the output -- Processing locus 81: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATAAAATTGGTAGGGCACAAAGTTTTAATATCATCCGCTCAAAAAACCAGAGAGATAGGGTCCCATTATAATTTTCCTC ATTTTCATATGCATTCAATAATCTATCTATATGTACAGTGACATAAGTGTCAGCCTTTACATCTATGTACATATAGCTTC TAGATTCTCATTCATTCCAATCACAGAGCTGATAATTTCAGCAGAATATTTACTATTTTTTATAAAGAAACAAAAAAACA TAGAAAGGTTTGGTTTTTCAAAGAACGTGAAAATGAGTATGCATTTTAGGAATAAAGCATGGATTATTCTGGCAATTCTG TGTTTTTCATCTTTGATTCACTCAACAGAAGCAGTGGTCACTTATGATCACAAAGCTTTAATCATCAATGGACAGAGAAG GATCCTTATCTCCGGTTCTATACACTATCCAAGAAGCACTCCTGAGATGTGGCCTGATCTTATAAAGAAGGCCAAAGAAG GAGGTTTGGATGTTATACAAACATATGTTTTCTGGAATGGACATGAACCTTCTCCTGGAAATTACTATTTTCAGGATAGA TATGATCTGGTTAAATTCACCAAACTGGTGCACCAAGCTGGACTATATCTTGATCTAAGAATTGGTCCATATGTTTGTGC CGAGTGGAATTTTGGGGGATTTCCGGTTTGGCTCAAATATGTTCCTGGTATGGTTTTCAGAACAGATAACGAACCTTTCA AGATTGCAATGCAAAAATTCACAAAGAAGATTGTGGATATGATGAAAGAAGAAAAGCTGTTTGAGACTCAAGGAGGACCC ATCATTCTCTCACAGATAGAGAATGAGTATGGACCAATGCAATGGGAGATGGGAGCAGCAGGAAAGGCTTACTCAAAATG GACAGCTGAAATGGCATTGGGACTGTCCACAGGTGTCCCATGGATTATGAGCAAGCAAGAGGATGCTCCTTACCCTATTA TAGACACATGCAATGGATTTTATTGTGAGGGTTTCAAACCAAACTCAGACAATAAGCCGAAGTTGTGGACAGAGAACTGG ACTGGTTGGTTCACGGAATTTGGAGGTGCGATACCAAATAGACCAGTCGAAGACATTGCGTTCTCCGTGGCTCGTTTCAT ACAGAACGGTGGTTCTTTTATGAATTATTATATGTATTATGGAGGGACCAACTTTGATAGAACAGCCGGTGTTTTCATCG CCACTAGCTATGACTACGATGCTCCTATTGACGAATATGGATTACTAAGAGAACCCAAGTATAGTCACTTGAAAGAGTTA CATAAAGTCATCAAGCTATGTGAACCTGCATTGGTCTCTGTTGATCCCACCATCACATCGCTCGGCGATAAACAAGAAAT TCATGTGTTCAAGTCCAAGACTTCTTGCGCTGCATTTCTCTCGAACTACGACACGAGTTCTGCAGCAAGAGTTATGTTTA GGGGATTTCCGTATGATTTGCCTCCTTGGTCTGTTAGTATTTTACCAGACTGCAAAACCGAATATTACAACACTGCAAAG ATCCGTGCACCAACCATACTTATGAAGATGATTCCTACAAGCACAAAGTTTTCCTGGGAATCGTACAACGAAGGAAGCCC TTCTTCAAATGAAGCTGGTACATTTGTTAAAGATGGTCTAGTGGAACAAATAAGCATGACCAGAGATAAAACAGATTATT TCTGGTATTTTACGGACATTACAATTGGTTCTGATGAGAGCTTCTTGAAGACTGGTGATAATCCTCTTCTTACCATTTTT TCGGCTGGGCATGCTCTTCATGTATTCGTTAATGGCCTGCTTGCAGGAACTTCCTATGGAGCACTGAGCAACTCAAAGCT CACATTTAGTCAAAATATCAAATTGAGTGTAGGCATCAACAAACTTGCTCTGCTTAGTACTGCAGTGGGTCTTCCGAACG CTGGTGTGCATTATGAGACTTGGAATACTGGTATTCTAGGGCCAGTCACATTGAAGGGAGTAAACTCTGGAACATGGGAC ATGTCTAAATGGAAGTGGTCTTACAAGATTGGTCTTAGAGGTGAAGCTATGAGCCTTCACACCTTAGCTGGAAGTTCAGC TGTGAAATGGTGGATCAAAGGATTCGTGGTCAAGAAGCAACCCTTGACCTGGTACAAGTCTTCTTTTGATACACCGAGAG GCAACGAACCACTGGCTTTGGACATGAACACAATGGGGAAAGGTCAAGTTTGGGTAAATGGACATAACATTGGACGCCAC TGGCCTGCCTACACGGCTCGTGGGAACTGTGGACGTTGCAACTATGCTGGAATTTACAACGAGAAGAAATGTCTCAGTCA CTGTGGTGAACCTTCTCAAAGATGGTACCATGTACCTCGTTCATGGCTAAAACCGTTTGGGAACCTTCTAGTTATATTTG AGGAATGGGGTGGTGATCCAAGTGGAATCTCTCTTGTTAAAAGGACAGCCAAATGAAGCTTCTTACTTCAAATTTTTTCC GGAAGCATGCAGAGATTGTCATGTACAGATATAGTTGCATTCATTGGCTCATTGTGGAAGAATATTATATATATA ................................................................................ ........N.....................N.................................N............... ................................................................................ ................................N.....i...................i..................... ...........................................N.......................N............ ..............................................i................................. .........N..............N...........N.....N..................................... ......................................N..........N.............................. ........i.............................i..i...................................... ......................N.....N.......i....i......i............................N.. ..........i............................N......i...............N................. .......N....i................................................................... ................................................................................ ...................i...........i......N......................................... .........N........N.................N........................................... .................N.............................................................. ..N......................................................................i...... ............N................................................................... .....................i.....i.................................................... ........N.......................N......................i........................ .................................N.............................................. ..........N........N...........N.......................N........................ ................................................................................ ............N............................................................N...... i.......N.......................................N............................... ......i......................................................................... .......................N........i........................i...................... ...........................................N........................i........... .....................N.......N............N..................................... ....N...............................................N........................... ......N.............N...................................................... Pos Score Pred ------------------------ 89 0.098 - 111 0.189 - 145 0.162 - 273 0.485 - 279 0.519 Yes 299 0.715 Yes 364 0.155 - 388 0.491 - 447 0.581 Yes 490 0.181 - 505 0.483 - 517 0.172 - 523 0.384 - 562 0.214 - 631 0.215 - 679 0.134 - 690 0.477 - 729 0.621 Yes 759 0.708 Yes 762 0.559 Yes 823 0.421 - 829 0.377 - 837 0.738 Yes 842 0.794 Yes 849 0.658 Yes 878 0.487 - 891 0.810 Yes 920 0.327 - 927 0.647 Yes 943 0.349 - 968 0.297 - 973 0.642 Yes 1140 0.640 Yes 1152 0.639 Yes 1159 0.228 - 1210 0.235 - 1219 0.156 - 1237 0.148 - 1298 0.324 - 1363 0.287 - 1434 0.646 Yes 1453 0.208 - 1542 0.541 Yes 1548 0.822 Yes 1609 0.179 - 1633 0.243 - 1656 0.657 Yes 1714 0.166 - 1771 0.164 - 1780 0.197 - 1792 0.247 - 1816 0.331 - 1933 0.136 - 1994 0.386 - 2001 0.692 Yes 2009 0.306 - 2049 0.390 - 2087 0.638 Yes 2184 0.446 - 2193 0.565 Yes 2218 0.789 Yes 2284 0.472 - 2309 0.590 Yes 2342 0.262 - 2350 0.407 - 2363 0.274 - 2405 0.487 - 2453 0.210 - 2487 0.069 - 2501 0.144 -

Explain the output -- Processing locus 82: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTACCAAACAGATAGATATATATATATATTATATTTGTATTTAATCCTTTGGTCTCTATATAAATACAACACGTCATAA ACTTTACTAGCCACAACTTAAAGTATTGAATACATGGAGAATCACAGCTGCCTTCAGAAAGATACGACGTCGGCGGCCAA ATTAGACGTCGAAACTGGAAACCTCCGGCAGAGAGATGAAACGAAGCCGTTTTATGCAAACTCACTGACGCCACGTGAGA TGGAATCTCTTGTTGCGATCTGCGATACCTTCATATCCTCCATCGACGACTCTGGAGTAGGACACGTGGATGATTGCGTC GCTGGATATTTCTCCGCCTCCGCTTCTCAGACCGGAACTCCCGACCGCGTGGCGAGGTTAATGAGTGAGAGACTTCACCA CCCAAAGAAGTGGATACTAAGAGCTGGATTGTGGTTACTCTCTACTTGGATCGGAAGTCTAGTCTTGTGTGGTTGGAGAA GTTTCACCGGCGAGTTTCCTTACTTCCGGAGATTCTGCCGGCTTCCGGAGAAACGGAGAGAAGAGATATTGCTGAATTGG TCTTCAAGCTATTTCTCACTTCTTAGAATGTTATTTAGGACCATCAAACTTATAAGTGCCCTTGTCTTCTTCACTCAGGT TGATGAAAAAGGAAGGAACTTGGCGTGGAAAGCAATAGGTTACAATGGACCCAGCCCTGATCATAGTGATCATGAAGTAG AACTAAACGAGGAGAAGAAGAAGAAGAAACCTGAGGAGATCTTTGGACCACTTTACAATGGCATCGTTGATCTAAAAAGC CCTAGAGAAGCCGTGGAAAAGAAGCTAGCCGGTCGTGGGTTCGCAGTTTCAAATCAAAAGAGGAATACAAACGGATCATC TATCTCTGACCCGGTGATGAAAATCCAGTGTGATGCTGTGGTGGTTGGGTCTGGATCCGGTGGAGGAGTGGCGGCAGGAG TGTTAGCCAAGGCTGGTTACAAAGTGTTGGTGATTGAGTCAGGGAATTACTATGCAAGAAGCAAACTTTCTTTACTTGAA GGGCAAGCTATGGATGACATGTATTTATCCGGTGGGTTATTAGCGACGTCCGATACGAACGTAGTGATTCTTGCGGGATC CACAGTCGGAGGTGGTTCTACAATCAATTGGTCCGCCTCGATCAAGACACCAGAACATGTGATGAAGGAATGGGCAGAAA AGTCAAAACTCGAAATGTTCGGAAGTGATTTGTATCGAGAGGCAATGGATGTTGTCTGTAAGAGGATGGGTGTTCAATGC GGATTTGTAGAAGAAGGGTTTAATAATGAGGTATTGAGAAAAGGTTGTGAAAAGTTAGGGTTACCAGTGAAGAATATTCC GAGGAATGCTCCATCCGATCATTACTGTGGGTTTTGTTGCTTGGGTTGCAAGAAAGGTCAAAAGCAGGGGACTTCAGAGA CTTGGCTTGTAGATTTGGTGGAATCTGATAATGGTTTAATCCTTCCAGGTTGTCAAGCGACGGAAGTCATGTACGACTGT GAACAGGGGAAGAAGAAGAAGGCAACTGGAGTTGCGTTTGCGTTTGGGGAAGAGATTTATGTGGTGGAGTCTAGAGTGAC AATAGTGGCTTGCGGTGCGCTTAGAACGCCGCATCTGTTGAAACGTAGCGGTTTGAAGAATAGTAACATTGGGAGAAACC TTTGTCTACATCCGGTTGTTATGGCTTGGGGATGGTTTCCAGAGGAAGATAAGTGGCCGGAAAAGAAGAAAAAGAGCTAC GAGGGGGGAATAATGACTGCGATGTCTAGCGTTGTAATTGAAGAAACGCATAGTTCTTATGGTGAAATGGTTATCCAGAC ACCTGCTCTACATCCAGGAATGTTCTCAGGGATCATTCCCTGGACATCAAGCAAGGATTTCAAAACCCGAATGCTCAAAT TTTCAAGAACCGCACACATTTTTGCGCTTCTGAGAGATAAAGGAACCGGAACAATAGATTCCAAGACCTACATTGACTAC AACTTAAACGATGAAGACGAAGAGAGTCTAAAGAACGGTCTTGAAAGAGTCTTGAAGATTCTGGCCGCAGCCGGAGCCGA GGAGATTGGGACGCATCATTCAGAAGGTAGGAGTCTGAATGTTAGAACAGCGAGTTCGTTAGAGATTGAGCGGTTTGTGA GAGAAGAGAGTTCAAAGCCGTTGAAGGATTTGTCAGGTCAGATATGTTCAGCTCACCAAATGGGAAGTTGTAGGATGGGA ATTAGGCCGGAGGAATCCGCGGTGAGGCCAACAGGAGAGACTTGGGAGGTCGAAAGGTTATTTGTGGCGGACACAAGTGT TTTTCCGACCGCTTTAGGGGTTAATCCGATGGTCACCGTTCAGTCTATTGCTTATTGTATTGGACTTAATGTTGTTGATG TTCTTAAGAAGAAGAAGTGAGGGTTCATCAGTTTGAAATAGATTTTAGTTTTCCACATATTTTTTAAAATGAAATGTTTT TTTTATACAATTGATAAAACTAATATTAAGAAGTTCTTT ................................................................................ .................................i.............................................. ...................................N.................N.........................i .....................................................................i.......... ............................................................N................... ................................................................................ ................................................................................ ...........................N.................................................... ..N.........................................i..........................N........ .........................................................i...................... ................................................................................ ................i...............N............................................... ...................................................N............................ .........i...N....i............................................................. ........................................................N....i.......N.......... ..............i.............................i...N................N..........N... .........................N...................................................... .....N.......................................................................... ..............................N.....................................i........... ..........................................................N..................... ................................................................................ ....................N..........i................................................ ............i........N....................................N.......N............. ...................N..................................................N......... ................................................................................ ..........N..................................................................... ......................................N......................................... ...........................................i...............i..............i..... ................................................................................ ............................N.......................................N........N.. ....................................................................N....N...... ....................................... Pos Score Pred ------------------------ 114 0.530 Yes 196 0.344 - 214 0.293 - 240 0.844 Yes 310 0.576 Yes 381 0.171 - 588 0.360 - 643 0.236 - 685 0.551 Yes 712 0.355 - 778 0.693 Yes 897 0.651 Yes 913 0.295 - 1012 0.241 - 1050 0.735 Yes 1054 0.362 - 1059 0.755 Yes 1177 0.207 - 1182 0.540 Yes 1190 0.400 - 1215 0.600 Yes 1245 0.723 Yes 1249 0.264 - 1266 0.250 - 1277 0.370 - 1306 0.319 - 1366 0.425 - 1471 0.237 - 1509 0.634 Yes 1579 0.250 - 1701 0.499 - 1712 0.590 Yes 1773 0.591 Yes 1782 0.238 - 1819 0.351 - 1827 0.480 - 1860 0.390 - 1911 0.414 - 2011 0.458 - 2119 0.263 - 2204 0.527 Yes 2220 0.647 Yes 2235 0.626 Yes 2349 0.353 - 2389 0.058 - 2398 0.134 - 2469 0.061 - 2474 0.042 -

Explain the output -- Processing locus 83: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTCGTTAAACTTAAAGTGATTTATACACATAGTATATTCAAATATCGATAAATGTATTTTGCTAATCAAATCGTATAT TCTTTTATACCAGTTTTTTAACGTCATCTCGCCAAAACAACAACAAATAGATGTAAAATAATGAAAGAAAACAAAAAACA AGATCCATGGGGAAGAGTCACATGCATGGAGCATGCAACTAGCTTACTTCATCCAATTCCCACTCAAAATTCTCTCTTTA TATATACACTTTCTCTACCACTACTTCCCTCAAAACAAGCTAACTCGATCCTTCTCACTAAAGCGATCGTACCGATCTTA ATATCTTTAGCGAGCGAGAGAGAGAGAGATAGAAATCCTGAGAGGAAAGAGAGGATGGAGATCGAAAAAAGCAATAACGG TGGTTCGAACCCATCTGCAGGAGAAGAATTTAAGGATATGATTAAGGGAGTGACTAAGTTCTTGATGATGGTGATTTTCC TTGGAACCATCATGCTTTGGATCATGATGCCAACACTAACCTATCGAACCAAGTGGTTGCCACATCTGCGTATCAAGTTT GGAACATCCACTTATTTTGGTGCCACAGGGACGACGCTTTTCATGTATATGTTCCCAATGATGGTCGTAGCTTGTTTGGG ATGTGTCTATCTTCACTTCAAGAATCGAAAGTCGCCACACCATATCGATAGGGAGACGAAGGGAGGAGTGTGGAGTAAAC TAAGAAAGCCAATGTTAGTGAAGGGGCCATTAGGAATAGTCTCAGTGACTGAGATCACCTTCTTGGCGATGTTTGTGGCT CTTCTTCTCTGGTGCTTTATCACTTATTTGCGCAATAGCTTCGCCACTATCACTCCTAAATCAGCTGCCGCACATGACGA ATCTTTGTGGCAAGCGAAGCTGGAATCAGCGGCGCTAAGGTTAGGACTGATCGGAAACATATGTTTGGCGTTCTTGTTCT TACCGGTGGCTCGTGGGTCATCGTTGCTACCGGCAATGGGATTAACCTCGGAGTCAAGCATCAAATACCATATATGGCTC GGCCACATGGTCATGGCCCTTTTCACCGTTCATGGTCTTTGTTACATAATTTACTGGGCCTCAATGCATGAAATCTCCCA GATGATCATGTGGGATACGAAAGGTGTCTCAAATTTGGCCGGAGAGATAGCTCTGGCGGCCGGACTTGTGATGTGGGCCA CCACATATCCGAAGATAAGGAGAAGATTCTTCGAAGTCTTCTTCTACACTCACTATCTCTACATCGTCTTCATGCTCTTC TTTGTCCTCCACGTCGGCATAAGTTTCTCCTTCATCGCTCTCCCTGGTTTCTACATCTTCTTAGTCGATCGTTTCCTTCG GTTTCTTCAGTCACGTGAAAATGTTCGCTTGCTCGCTGCTCGGATTCTTCCTTCCGACACTATGGAGCTCACTTTCTCCA AAAACTCTAAGTTGGTTTATAGCCCGACGAGCATAATGTTTGTGAACATACCGAGCATTTCAAAGCTGCAATGGCATCCG TTTACGATAACTTCCAGTAGCAAACTCGAACCAGAGAAGCTAAGTATTGTTATCAAGAAAGAAGGCAAATGGTCAACTAA GCTTCACCAGAGGCTTTCTTCTTCTGATCAGATCGACCGACTTGCTGTTTCTGTAGAAGGTCCTTACGGCCCTGCTTCCG CCGATTTCTTAAGGCATGAAGCTTTGGTTATGGTGTGCGGAGGAAGCGGGATAACTCCGTTTATCTCTGTTATTCGTGAT TTGATCGCCACAAGCCAAAAGGAAACATGCAAAATCCCAAAAATAACTCTCATTTGTGCGTTTAAGAAGTCTTCAGAGAT CTCCATGCTCGATCTTGTCTTGCCATTATCCGGTCTTGAGACTGAACTATCCTCCGACATTAACATCAAAATCGAAGCCT TCATAACCCGAGATAATGATGCAGGAGATGAAGCAAAAGCTGGGAAAATCAAAACTCTCTGGTTCAAACCAAGTCTTTCA GACCAATCCATCTCATCAATCCTCGGACCAAACTCATGGCTTTGGCTCGGTGCAATTCTCGCATCTTCGTTCTTGATCTT CATGATAATTATTGGTATCATCACTAGGTATTACATTTACCCGATCGACCACAACACCAACAAGATCTATTCTTTGACTT CAAAAACCATCATATATATCTTGGTCATCTCCGTGAGCATAATGGCGACTTGTAGTGCGGCTATGTTGTGGAACAAGAAG AAGTATGGTAAAGTTGAAAGTAAACAGGTCCAAAACGTCGACCGTCCATCTCCAACATCTTCTCCTACCTCATCATGGGG TTACAATTCCTTGAGAGAAATCGAAAGTACGCCACAAGAATCGCTCGTGCAACGCACCAATCTGCATTTTGGAGAAAGAC CTAATCTCAAGAAACTTCTACTTGATGTGGAAGGTTCGAGTGTGGGAGTTCTTGTTTGTGGTCCTAAGAAGATGAGACAA AAGGTTGCGGAAATATGTTCCTCTGGTTTGGCTGAGAATCTTCATTTTGAATCTATCAGTTTCAGCTGGTGATTGCGTAT ATAACTCTGTTTTCACTATGTGTTGTGCAATTTGCTTGAGTTGCAAGTTAATAAGGTGGCCCTGTGATTTGACCTATCTT TGTGTGCTTTA .....................................................i.......................... ..................................................N.........N................... ......N..............N...N......N............................................... ................................................................................ ......................................................N......................... .....................................N..........................N..N............ ...........N...........N..N..................................................... ..........................................N.....N........i..N................... i............................................................................... ...........N........................................................i........... .........................................................................N...... ............................................................i................... ...................................N.....................................i...... ......i.....N..................N...............................i...N............ .......................................................................N........ ................................................................................ ....................N........................................N.................. ...................................N..................................i......... ....................................................................N........... ................................................................................ ...............N.............i.................................................. ..........................i..................................................... ....N........................................................................... ...............i..i........N.................................................... ...................................i............................................ .........................................i....................i................. ....N.....................................................................N..... ................................................................................ ........................N..............................................N........ ..............N................................................................. .................N.............................................................. ........... Pos Score Pred ------------------------ 54 0.820 Yes 131 0.236 - 141 0.422 - 167 0.401 - 182 0.399 - 186 0.420 - 193 0.232 - 375 0.492 - 438 0.283 - 465 0.156 - 468 0.304 - 492 0.186 - 504 0.117 - 507 0.275 - 603 0.300 - 609 0.409 - 618 0.779 Yes 621 0.384 - 641 0.702 Yes 732 0.127 - 789 0.539 Yes 874 0.398 - 941 0.691 Yes 996 0.171 - 1034 0.614 Yes 1047 0.510 Yes 1053 0.353 - 1072 0.356 - 1104 0.657 Yes 1108 0.208 - 1122 0.592 Yes 1128 0.794 Yes 1191 0.183 - 1272 0.237 - 1381 0.413 - 1422 0.206 - 1476 0.453 - 1511 0.780 Yes 1589 0.368 - 1696 0.199 - 1710 0.708 Yes 1787 0.692 Yes 1845 0.413 - 1936 0.508 Yes 1939 0.527 Yes 1948 0.339 - 2036 0.791 Yes 2082 0.125 - 2202 0.601 Yes 2223 0.508 Yes 2245 0.460 - 2315 0.468 - 2425 0.126 - 2472 0.132 - 2495 0.122 - 2578 0.138 -

Explain the output -- Processing locus 84: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAACACTATCAAGGAACCAACACTCTTTTTAAAAGTCATGAAAGATTATAATGTTGATATGTGTGTGTTTTGTTTTTTGT GTTCTTCAGCTTGATAGTTAAATGGATCCTGTTCCTGTTCGATGTCTTCTTAACAGTATATCTCGGTATCTTCATCTGGT TGCGTGCCAGACTATAAGATTTAATCCTATTCAAACATGTATTGGAAATATGGTTCTCTTGTTGAAGCTCTTGAAACCGT TGCTCGATGAAGTTGTTGATTGCAAGATACCTTCTGATGACTGTTTATATAAAGGATGTGAAGACCTTGATTCTGTTGTT AACCAGGCTCGGGAGTTCTTAGAGGACTGGTCACCAAAGTTGAGCAAGTTGTTTGGTGTGTTTCAATGCGAGGTTTTGTT GGGAAAGGTCCAGACTTGTTCGTTGGAGATTAGTCGCATACTTCTTCAGTTATCACAGTCAAGTCCGGTTACTTCAAGCG TACAAAGTGTTGAGCGCTGCGTGCAGGAGACTGAGAGTTTTAAGCAAGAGGGGACATTAATGGAACTCATGGAGAATGCT TTACGGAATCAGAAAGATGATATTACCTCTTTGGATAACAATCATCTGGAAAGCATAATTCAAATGCTTGGATTGATATC AAACCAAGATCTCTTAAAGGAAAGCATTACTGTGGAGAAAGAGAGGATAAGATCCCAGGCCAGTAAGTCAGAAGAAGATA TGGAACAAACCGAACAGTTGATAGAACTCGTCTTGTGCATCCGTGAACACATGCTTAAAACTGAGTTTCTTGAAGTGGCT AAAGGTATCTCGATACCCCCGTATTTCCGGTGTCCTTTGTCAACAGAACTCATGCTGGATCCGGTAATAGTAGCTTCAGG ACAGACATTTGACAGAACATCCATTAAGAAATGGCTTGATAACGGGTTAGCTGTTTGTCCAAGGACGCGGCAGGTGCTGA CTCATCAAGAACTCATTCCCAATTACACGGTTAAGGCTATGATAGCGAGTTGGTTGGAGGCAAACAGGATCAACCTTGCT ACTAACTCTTGTCATCAGTATGATGGTGGTGATGCTTCATCCATGGCTAATAATATGGGTTCTCAAGACTTTAACCGCAC CGAGAGTTTTCGTTTTTCTTTACGGAGCAGCAGTTTAACCTCAAGATCATCTCTTGAAACTGGAAATGGGTTTGAGAAAC TGAAGATTAACGTGTCTGCCAGTTTATGCGGGGAATCTCAAAGCAAGGATCTTGAAATATTCGAGCTTTTGTCTCCGGGG CAGTCTTACACTCACAGCAGGAGTGAATCAGTTTGCAGTGTTGTCTCGTCTGTTGATTATGTACCTTCGGTGACACATGA GACAGAAAGTATACTAGGGAATCACCAAAGCTCCAGTGAGATGTCTCCCAAGAAAAACTTAGAAAGTTCAAACAATGTAA ATCATGAGCATAGCGCAGCAAAGACTTATGAGTGTTCTGTACATGATTTAGATGATTCAGGAACAATGACGACTTCACAT ACCATAAAATTGGTAGAAGATCTTAAAAGCGGGTCTAACAAAGTGAAGACTGCTGCTGCAGCTGAAATACGTCATCTCAC CATTAACAGCATTGAAAATCGTGTTCACATCGGGCGTTGTGGTGCTATTACTCCACTGCTGTCACTTTTATACTCAGAAG AAAAGCTAACTCAAGAACACGCAGTCACGGCTCTTTTGAATCTTTCCATCAGTGAACTAAACAAAGCCATGATTGTGGAA GTCGGGGCGATAGAACCGCTTGTTCATGTTTTGAACACAGGAAATGACAGAGCCAAAGAGAATTCAGCAGCATCATTGTT CAGTCTGTCTGTTCTGCAGGTCAACAGAGAACGAATAGGCCAGTCTAACGCAGCGATACAAGCTCTGGTGAATCTTCTTG GTAAAGGAACATTTAGAGGAAAGAAAGACGCCGCCTCTGCTTTGTTCAATCTATCGATTACTCATGATAACAAGGCCCGT ATCGTGCAAGCTAAGGCGGTTAAGTACCTTGTGGAGCTGTTAGACCCAGATTTAGAGATGGTTGATAAAGCAGTTGCTCT TCTTGCAAATCTTTCTGCAGTTGGAGAAGGGCGTCAAGCCATCGTGAGGGAAGGTGGGATTCCATTACTTGTTGAAACTG TTGACTTAGGATCTCAGAGAGGGAAAGAGAATGCAGCTTCTGTGCTGCTTCAGTTGTGTCTGAACAGTCCCAAGTTTTGC ACTCTGGTCTTGCAAGAAGGCGCCATACCTCCGCTTGTTGCCTTGTCTCAGTCTGGTACACAGAGAGCAAAAGAGAAGGC ACAGCAACTTCTTAGCCACTTCCGAAACCAGAGAGATGCAAGGATGAAGAAAGGTAGATCATGATTCATGAAGAGATACC ATTTTAACATTTCCCTCGGGAAATTATTTTGCTAGAAATTTTTATTTTTTCTTTCATTTTGCCAAGAACATTAGCTGGAG AAT .....................................N............i.......N..................... .....................N...................N...................................... ....................................N............i.............................. ......N.............................N..................N........................ .................................................................i.............. ................................................................................ ...........................................................N........N......N.... ................N..............................................N................ ...............................................................................i ..................................................N............................. ...................................................i............................ ..............................i................................................. ......................................N......................................... ...................N..N........N..........i...........i......................... .................................................................N.............. .........................N...................................................... ..........................................................N.................N... ........................................i.................................N..... ...N.......................N..............N........N.............i.............. ................................................................................ ................................................................................ ....................................................................i........... .........................N.................N.................................... ................................................................................ ...............................................................N................ .........................................................i...................... ................................................................................ ..............................N................................................. ................................................................................ ...................................N.......N................N......N............ ................................................................................ ... Pos Score Pred ------------------------ 38 0.171 - 51 0.699 Yes 59 0.439 - 102 0.365 - 122 0.222 - 197 0.416 - 210 0.539 Yes 247 0.344 - 277 0.252 - 296 0.306 - 386 0.558 Yes 540 0.295 - 549 0.259 - 556 0.167 - 577 0.276 - 624 0.454 - 720 0.753 Yes 771 0.135 - 852 0.581 Yes 911 0.835 Yes 999 0.468 - 1060 0.248 - 1063 0.279 - 1072 0.267 - 1083 0.561 Yes 1095 0.682 Yes 1186 0.295 - 1226 0.159 - 1339 0.161 - 1357 0.279 - 1401 0.647 Yes 1435 0.301 - 1444 0.162 - 1468 0.219 - 1483 0.112 - 1492 0.072 - 1506 0.833 Yes 1749 0.515 Yes 1786 0.447 - 1804 0.425 - 1984 0.061 - 2058 0.663 Yes 2191 0.242 - 2356 0.282 - 2364 0.240 - 2381 0.346 - 2388 0.116 -

Explain the output -- Processing locus 85: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGTGCTATTCAATAATGTTCTGTTTGATTTCGATGGAATAATTTAGTGTGACTGATGTTGCTTTTATGTCGCTTATGTA TTATTGGTGATCTATGTGTAAAACATGTGTTGCAGGATAGTAATGAACCGAAGTAAAGAAGAGAACGTTGCTCCGACAAT GAAAGATGATAGTCCATTTGGAAAGCTTACAGAGGATCTCTTGATAGAGATATTTATCAGAATTCCAATAACAAATTGGG AACAAGTATCGTGTGTTAGAAAGCAGTGGGCTAATTTATTCCGCGGAGAATGCTTATGGCTGGCTGCTCTTAATCGGGCG TATCCACTTGCTAGCAAAACTAAGAGCTGGATTGGACCAATTCGTCAAGGATTAAGCAAACGGAGATATGTGGCTTTATA CATCAGCAGAAACATATTAGGTGTGGATGATACAGACATAGATGAGATGCTTGGACATATTTACGTGTTCTTGAATGATC AGCTTCAACTTTCCACTATGCCTGCTTCAGGCATTTTGCATGGAACCCTTATCGACCAATTGATTGTTTGTGGCCAATCG AAAGAAGAAGCTGGTGAGCTTGCAACAAAGATTTGGCTGGCTCTTCTTGACAATTTAGAGGACACAAAACATACATTTAC CGTGCTGAAATCAATCGCACAAGAATATGATGGCTTTCTTCCATATCCATATTCAAGACCAATCAAAGTGCAGTGGAAGG TGTTCGAGAAACTGTTTGTAGATTTCCGTGACTTGCTTGATCATTCAGAGTACTGCGACTTAATAGGAATTGCCAAAAAT AAGTTTCAAACCATACCTTATTTCAGTTATCCATCCACTTTATTCTTCTTCTTGGAAATGGCTCTCATGAAAGTAATGAC GATTCTGGTTCTCTTCGTCTCGGTGTCATCGACCTTGGCGCAATCCAACAATGGCGGTCACATTTCGATAATCGTCTCGG AAACAGGTCTTGAATTTGCTAAAGATTACCTCATCAAGAAAGTGATCACTACGACGCTTCCACTTCAGCTACCAGACATT GAGAATAAGGTTAAGATCCCTCTAATCGGGAAAGTTCGAATGGGTCTATCGAATATTCAGATTGATGCAGTTCATGTCCA GTCTTCGAAGATGGAGACTCGAAAAGATGGAATCATTTTGAGTGTTTTAGGTGCTACAGCAAATTTGAGTATGGACTGGT CTTATACTTACAGAGCTTCCTTCTTTGAGATTTCTGATCATGGAGATGCTTCTGTTGAGGTTAAAGGAATGAATGTGAGA ATCACTGCCACTTTGGTTAATGATAATGGAAGTCTAAAGATTGCCTCACGGGAAAATGATTGTACAGTAAAGAACATTGA TATTCATATCAATGGTGGTGCTTCTTGGCTATATCAAGGGGTGGTTGATGCATTTCAAAAAATGATTATATCTACTGTTG AAAAAACTGTCTCTACTAAAATTGTAGAAAAAATGAAGAAGCTTGATTCTTTCTTGCAATCACTTCCAAAACAGAGAAAG ATTGATGACTCTGCTGCAGTGAATCTCACTTTTACAGGCAACCCTGTCTTAGGGAATTCGTCGGTTGAAGTTGACATCAA TGGTTTATTCATGCCAAAGGGTGATGATATTAAAGTTGCAGGGTCTCGTTCTTCTTCCTTCTTTGGTGGGGTTAATAAGA GAATGGTGACAATTTCAGTAGAAGAAGGAGTTTTCAACTCTGCAACACTTGTCTACTTCAACGCTAAGGTGATGCATTTA GTTATGGAGGAAACAAAGAACGGGTCCATTCTAAGCACATCTGACTGGAAACTCATCCTTCCAGAGCTGTACAAACATTA TCCAGATAATAAAATGGTGCTTAACATGTCAGTAACATCTCCTCCTGCTGTTAAAATCACAGAGAATGGAATTGATGCGA CGATTCAGCTAGATATAGCGTTCGATGTTCAAGACTCTGGAGAAAATCTATCTGTAGCACGCCTATCAACAATTCTGAGT GTTGCGTGTTCTACAGAAATCGTAAAGAATAATCTAATCGGTAGCCTCAGATTAAATGATTTCAATGCAACAATGAAGTG GAGTAAAATTGGAGAGTTTCAAACAAACTATGTTCAGGCTGCTACGTCTAGGATTCTTGAAGCCTTGTTTTTGCCGTACG TAAACACACGTCTCAAGAGAGGATTCCCTTTGCCGATTCCCGGCGATTTCACGATCAAAAACATAAAGATTGTTTATGTT AATAGTGGCATTTTGGTATGTACCGATATCGGCACTAGCACAAACCAGTAAGCAAGTATTATATAGCTTCTTAGATTGCA TGTACGTAAGCCTGAAGAAATATAATGACAACCATAATTGTGATTTGAACCGTTTGGAACTTCCCCTCTA ...............i.................N.....................N..........N........N.... .............N..........i.................N...................................i. .....N.......................................................................... .................................................N.....N........................ ...................................................................N............ ..........................N..............N....N...........................N..... .................i.....................N........................................ ................................................................................ ..........................i..N.................................................. ................................................................................ .........................................................i........i........i.... ..................................................N............................. ................................................................................ .......................................N........................N........N...... ..........N...............N...........................................i......... .......................................N.....N......................i...N....... ...................N.....N.............................N........................ ...........N...................................N.............N.................. ................................i............................................... ....i..........................................................................N ..........N............N........................................................ ..N....................................................................N........ ...i............................................................................ .............i...........i.......................................N........N..... ........................N....................................................... .......................................................N........N.......N....... .............................N.................................................. ...........................................................................N.... .................N.............................................................N ........................N............................................. Pos Score Pred ------------------------ 16 0.595 Yes 34 0.181 - 56 0.369 - 67 0.093 - 76 0.177 - 94 0.236 - 105 0.553 Yes 123 0.221 - 159 0.551 Yes 166 0.271 - 290 0.392 - 296 0.342 - 388 0.356 - 427 0.138 - 442 0.182 - 447 0.204 - 475 0.165 - 498 0.751 Yes 520 0.292 - 667 0.503 Yes 670 0.378 - 858 0.808 Yes 867 0.504 Yes 876 0.576 Yes 931 0.410 - 1080 0.433 - 1105 0.146 - 1114 0.173 - 1131 0.487 - 1147 0.245 - 1191 0.802 Yes 1240 0.114 - 1246 0.156 - 1269 0.768 Yes 1273 0.194 - 1300 0.128 - 1306 0.257 - 1336 0.257 - 1372 0.158 - 1408 0.082 - 1422 0.391 - 1473 0.751 Yes 1525 0.592 Yes 1600 0.259 - 1611 0.474 - 1624 0.087 - 1683 0.439 - 1752 0.326 - 1764 0.500 - 1854 0.886 Yes 1866 0.746 Yes 1906 0.255 - 1915 0.207 - 1945 0.218 - 2056 0.105 - 2065 0.125 - 2073 0.395 - 2110 0.202 - 2236 0.210 - 2258 0.349 - 2320 0.062 - 2345 0.121 -

Explain the output -- Processing locus 86: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTAAATCCATCCTTCAGATCCAGAAATCAACACAATCTTTAACGATTTCTTGCATCACAAGCAACAAATCGGGTATTGGT AGGTAAGATTTGGTTAATTAATGCATGAGAAAACCACATAGGAGAGGAGGAACAATGGCAATGGAGAAGTCTAGCTCAAA GTACAGAACCCAGTTCTGGTACGTGGCAATGGCCTCATTCCTCCTTTGGCTTGTCTTGCTTTACCTTTTTAGTTCTTCCG CTAAAACCGTTCATAACCACGAGAGATTGTTCCGGCAAGAAAATATCATCGATCTCCCTGTCGAAAACGTCCCACAAAAC CACGAACCTGATGAAGCTCCTGTCGTTAACGTTGTCTCTGAGGACATTTCCGTTGATAACAGCTCTCTTCCTGTTTCTGT CGATTCTCCTCCGGATGATCAGATGTCAGAAGACAAGAAGGTTGTGACTGATCTCGTTGAAGAACTTGAGAAAGAAAGGG TTGAAAATGAGAAGAAGAGAGTTGATTCGGTTGTAATTGGACGGTCATCTAAGGCAAGGAGAGGACATCGAGAGCCACGA AAGGCGAGATCTTCGGGTAGATTGGAGGCAGAGAAGAAAAGGGTTCGTCGTAATGATGCTGATGAGAGCAATGCGGAGAA TAGTGATGAGAATCATCAAGCATTAGACAAAGAACCGAACTTTTTCGAGGCTAAGAAAGACGTGGAACCAAAGAAAGCGC TAGTTGATGATAATGACGACGATCTTGAGACCAAGAGAGGCAAGGAGTTGCCGAATGATTCTTCCAGTAATGTTGTTGAA GATGATAATGACGACGATCTTGAGACCAAGAAAGGCAAGGATAACATCTCTAAATCTGAACCAAAAACTCAGAGAAATTT TGTATTGAATAAGAATAATACTTCCAAGGCGAAAAACAGAGTAATTTCCAAGAGAAACCAACCTAGTAGAGTCGGTAAAG TTATGTTACGGCCAAGAGAAACGCGAAGTAACGATCCATGTAAAGGAAAATATGTGTATATGCATGAGGTGCCAGCTTTG TTTAACGAAGAGCTTCTCAAGAACTGTTGGACATTGAGCAGGTGGACTGATATGTGCGAGTTAACGTCGAATTTCGGGTT AGGCCCTCGTCTACCAAACATGGAAGGAGTTTCTGGTTGGTATGCCACAAACCAGTTCACATTGGAAGTAATATTCCACA ACAGGATGAAGCAGTACAAGTGCTTGACCAAAGACTCGTCTTTAGCTTCTGCGGTGTATGTTCCTTACTATCCGGGTTTA GACTTGATGCGGTTTTTGTGGGGACCGTTCCCTTTCATGAGAGATGCTGCCGCGCTTGATCTCATGAAGTGGCTGAGGGA GAGTCAGGAGTGGAAGAGGATGGACGGACGAGATCATTTTATGGTGGCTGGTCGAACCACTTGGGACTTTATGCGTACAC CAGAGAATGAATCCGATTGGGGAAATAGGTTAATGATATTACCGGAAGTAAGAAACATGACGATGCTACTAATCGAATCA AGCCCATGGAACTACCACGGGTTCGCGGTTCCTTACCCGACCTATTTCCACCCTTCAACATACGCAGAAATCATCCAATG GCAGATGAGAATGAGAAGAATTAATCGTCGTTACTTATTCTCATTTGTCGGTGCCCCACGACCAAACCTCGGTGACAGCA TAAGAACCGAGATCATGGACCAATGCAAAGCTTCAAAACGAAAATGCAAGCTTCTAGAATGCATATCGGGTTCTCAAAAA TGCTACAAACCCGACCAAATCATGAAATTCTTTTTGAGCTCAACGTTCTGTCTTCAACCACCAGGTGACTCATACACACG TCGCTCCACTTTTGACTCCATCTTAGCAGGTTGTATACCAGTTTTCTTTCATCCGGGATCAGCTTATGCGCAATACATTT GGCATTTACCGAAAGATATCGCGAAATACTCTGTTTTTATTCCGGAGAAAAATGTGAAAGAAGGTAAAGTGAGTATAGAG AATGTTTTGAGTAGAATACCGAGGACAAAAGTTTTCGCTATGAGAGAACAAGTGATAAGACTGATACCGAGGTTAATGTA TTTTCACCCGTCGAGTAAGAGTGAAGACACGGGAAGGTTTGAAGATGCATTCGATGTCGCGGTGGAAGGAGTTTTGGAAA GAGTGGAGGGGTTAAGGAAGAGGATAGAGGAAGGCAAGGAGGAGATTTTTGATTTTCCGGAGCAGTATTCTTGGAAATAT AATGTGTTTGGGAATGTTGAGAGGCATGAGTGGGATCCTTACTTCGATCGGCCTTAATTGTTTGTGTGTTTTTTTTTTTT TTATTGGGAGACAAGTGAATGTAGTTGTGATTGTCATCGTAGAAAGAAGTCACGAGGCCTTATTTTCTTAAATTGG ................................................................................ ....................N...N.............................i.....N................... ............................N................................................... ................................................................................ ..........N..................................................................... ..............N.......i......................................................... ......N......................................................................... ....................................................N..N.....N........N......... .....N.......................................................................... ......N.....N.........................................N..............N.......... ................................................................................ ..i..................................i.............N.......N...N................ ...................................................N............................ ...................N.....................N...................................... .....N...................................................N...................... ......i.............................N......N...................i................ ...................N....................N.............................N......... ......N.........................N.......................i.....N................. .....N.......................................................................i.. ....N.....i..................................................................... ..............N.......N....................i..............N....................N .....................N.......................................................... .................................................................N.............. ...................................................N............................ ............................................N........N.......................... ................................................................................ ..................N......................................................... Pos Score Pred ------------------------ 101 0.431 - 105 0.440 - 135 0.617 Yes 141 0.345 - 189 0.346 - 331 0.344 - 415 0.050 - 423 0.830 Yes 487 0.392 - 613 0.357 - 616 0.228 - 622 0.223 - 631 0.157 - 646 0.139 - 727 0.134 - 733 0.215 - 775 0.183 - 790 0.132 - 802 0.162 - 808 0.142 - 963 0.621 Yes 998 0.664 Yes 1012 0.298 - 1020 0.371 - 1024 0.364 - 1092 0.482 - 1140 0.449 - 1162 0.092 - 1206 0.220 - 1258 0.098 - 1287 0.578 Yes 1317 0.457 - 1324 0.340 - 1344 0.550 Yes 1380 0.487 - 1401 0.305 - 1431 0.153 - 1447 0.457 - 1473 0.443 - 1497 0.623 Yes 1503 0.400 - 1526 0.392 - 1598 0.738 Yes 1605 0.311 - 1611 0.622 Yes 1695 0.292 - 1703 0.361 - 1724 0.595 Yes 1739 0.346 - 1760 0.489 - 1782 0.316 - 1906 0.185 - 1972 0.229 - 2002 0.408 - 2040 0.365 - 2076 0.431 - 2125 0.219 - 2134 0.107 - 2242 0.167 - 2254 0.270 - 2266 0.165 - 2339 0.054 -

Explain the output -- Processing locus 87: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTAAACACTGTTAAACAAAATGACATGAGCAAGAGCAACCCATCGAACAAAACAAAGTGTTTTCAAAACACTATTAAAC ACAAGTAACTAATATGAATAAATGAAACTAGCCGTTGGAGCCCATTTACTAATATTAGGCCAGAACAGAGAGATCCACTG TTCCCACCCCCCAACCTCTTATTACCATCACCTGATCTTTGTCTCCCTTCTCTCTCTTTCTCTCACTTCTCTCTCACTAC AAGATAAAAGAACACAGACCAAGATTCTCTCTCTCTCTCTAAGATGGTCACGTGCTCTGTTGTTCTTTTCCAACGAAACC CATCTCTCTCTTTACAAAACTCTTTCAAATCCAAAATCTTTCCCTGTTCCATCCTCATCACAACTACTATTCACCCATAA AGAACTCCAATTATCTTCTTTCTTCTCTCTTCTTCTTCTTCTTCATCTTCTAATGGAGTTTATCACCCAAAACCAAGCTA TTACTTCTCTGAGTATGATCAACACCGACATTGATCAACCTAAGGCTTCACTTCGAAGCCGATTTCTCCTTCATCTAATC ATATGCCTCCTCTTCTTCGTCCCTCCCTGCTCAAGCCAAGCCTGGGATGGTGTAGTGATAACACAAGCTGATTACCAAGG TCTTCAAGCAGTTAAACAAGAACTGATTGATCCAAGAGGCTTCTTGAGAAGCTGGAACGGTTCTGGATTCAGTGCTTGCT CTGGAGGCTGGGCTGGAATAAAATGTGCTCAAGGTCAAGTCATCGTCATTCAACTTCCATGGAAGAGTCTTGGAGGTAGA ATCTCTGAGAAAATCGGACAGCTTCAAGCTCTTCGTAAGCTTAGTCTTCATGACAACAACCTTGGAGGTTCGATTCCAAT GTCATTGGGACTCATTCCTAATCTTAGAGGAGTTCAGCTTTTCAACAACCGTCTCACTGGCTCAATCCCTGCTTCTCTCG GTGTATCACATTTCCTTCAAACGCTTGATCTCAGCAATAACTTGCTCTCTGAGATTATTCCACCGAATCTTGCTGACTCT TCTAAGCTTCTTAGGCTTAATCTCAGCTTTAATTCACTGTCTGGTCAAATCCCAGTGAGTCTCTCTCGGTCTTCTTCCCT TCAGTTTCTTGCTCTTGACCATAACAACCTCTCTGGTCCAATCTTAGATACTTGGGGTAGTAAGATCCGTGGAACCCTAC CCTCTGAGCTAAGCAAACTCACTAAGCTTAGAAAAATGGATATTAGTGGTAACAGTGTTAGTGGCCATATCCCTGAAACC CTAGGGAACATTTCTTCTCTTATACATTTGGATTTGTCTCAAAACAAACTCACCGGAGAGATTCCTATTTCGATTTCTGA CCTGGAAAGCCTGAACTTCTTCAATGTCTCTTATAACAACTTATCTGGTCCTGTTCCTACTCTCTTGTCCCAAAAGTTCA ATTCCTCTTCTTTTGTCGGGAACTCACTGCTTTGCGGATACAGCGTTTCGACACCATGTCCTACTCTTCCTTCACCGTCT CCTGAAAAAGAAAGGAAACCATCCCACAGGAATCTGAGTACCAAAGACATCATCCTCATTGCGTCTGGAGCACTCCTCAT AGTTATGCTTATCCTTGTTTGTGTTCTATGTTGCTTGCTGAGGAAGAAAGCCAATGAAACCAAAGCCAAGGGCGGAGAGG CTGGACCTGGAGCTGTAGCCGCAAAGACTGAGAAAGGAGGGGAAGCTGAAGCTGGAGGTGAAACTGGAGGGAAGCTGGTT CATTTTGATGGACCAATGGCGTTTACTGCAGATGATCTTTTGTGTGCAACAGCAGAGATAATGGGGAAAAGCACTTATGG GACTGTGTACAAAGCTACACTTGAAGATGGAAGTCAAGTAGCAGTGAAGAGATTGAGAGAAAGATCACCAAAAGTCAAAA AGAGAGAGAAGCTTGTCGTCTTCGATTACATGTCTAGAGGAAGCCTTGCCACGTTTCTCCATGCAAGAGGACCAGATGTA CATATCAATTGGCCTACAAGGATGAGCTTAATAAAGGGAATGGCTCGAGGCTTGTTCTACCTTCACACACACGCAAATAT CATCCACGGAAACTTAACATCAAGCAATGTGCTTTTGGATGAGAACATAACCGCGAAGATCTCAGATTACGGTCTCTCAA GGCTAATGACAGCAGCAGCAGGATCCAGCGTGATTGCAACAGCTGGTGCGTTAGGTTACAGAGCACCTGAGCTCTCTAAG CTGAAGAAAGCCAACACGAAAACCGATGTGTACAGCCTCGGTGTGATCATATTGGAACTGTTGACTGGGAAATCTCCGAG TGAGGCCTTAAACGGTGTGGATTTGCCTCAATGGGTTGCTACTGCGGTTAAAGAAGAGTGGACTAATGAGGTTTTTGATT TGGAGCTGTTGAATGATGTGAACACAATGGGTGATGAGATTTTGAATACACTGAAACTGGCTTTACATTGTGTTGATGCT ACACCATCAACAAGACCTGAAGCTCAACAAGTGATGACACAACTTGGAGAGATTAGACCGGAAGAGACAACAGCAACAAC ATCAGAACCGTTGATTGATGTCCCTGAAGCTTCTGCTTCCACAAGTCAATAGAAAGAGTGGAGAATTTTTGTAGTGTGGA AATGTTGCAGAGTAGTAGTTGATTTCTTTTGCAATTCTTTAATTCTTCCAAGTTGTTTTTTTGTTCTTTGT ....................i....N...................................................... .............i.......N.......................................................... ................................................................................ ...........................................N.................................... ................................................................................ ....................................................i........................... ..............N................................................................. ..i...........................................i................................. ................................................................................ ......................N...................................N..................... .................................................N............................N. ................................................................................ ................................................................................ ................................................................................ ................................................................................ ...................................i............................................ ................................................................................ .......................i........................................................ .......................................................i........................ ................................................................................ ....i......................N.........................i.......................... ................................................................................ .......N.......i...............N............................i...............N... ..........................N..................................................... .............................N..............................N..............N.... .....................i.................i........................................ ..........................N...........N......................................... .....N.......................................................................... .........................N...................................................... ..............................N..................................N.............. ............N..N..........i......N.........................................N.... .................................i.............................................. .................N.............................................................. Pos Score Pred ------------------------ 21 0.695 Yes 26 0.336 - 94 0.564 Yes 102 0.445 - 284 0.350 - 453 0.556 Yes 495 0.438 - 563 0.647 Yes 607 0.546 Yes 743 0.335 - 779 0.380 - 850 0.119 - 879 0.438 - 1236 0.772 Yes 1384 0.779 Yes 1496 0.608 Yes 1605 0.570 Yes 1628 0.392 - 1654 0.655 Yes 1768 0.169 - 1776 0.665 Yes 1792 0.111 - 1821 0.720 Yes 1837 0.468 - 1867 0.228 - 1950 0.449 - 1981 0.199 - 1996 0.250 - 2022 0.538 Yes 2040 0.575 Yes 2107 0.232 - 2119 0.456 - 2166 0.465 - 2266 0.320 - 2351 0.206 - 2386 0.121 - 2413 0.390 - 2416 0.195 - 2427 0.649 Yes 2434 0.130 - 2476 0.197 - 2514 0.512 Yes 2578 0.192 - 2642 0.054 -

Explain the output -- Processing locus 88: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAGATGTCTGTAGTCTTGGTTTGTTGTTGATTTGCTTTAGGTCTGTCTTTGCTTATTGTTGTAATGTTTTGAGTTCTGG CCACATTTATTTCCTTACTGAACGGCTCTTCACATCTTGTTTCTTCCGCATTTTGATAGGACGAGGCAAATGAGAAGTTA CAACTTGTTTCACGAAAGCAATCCCACAATCAGTTTTAGTTTTGTTGGTGCAGAAACGATGGCACCTAATCTGCATATCA AGAAAGCATTTATGGCTATGAGGGCTATGGGAATAGAGGATGCGCGAGTGAAGCCGGTCTTGAAAAACCTTCTCGCCCTT TACGAGAAAAACTGGGAGTTAATTGCTGAAGACAATTACAGGGTTCTTGCTGATGCTATCTTCGATAGCCATGAAGATCA GGCTATTCAAGAAAGCGAAGAAAAGAAGGCAGACGAAGTTAAAGAGGATGAAGGCTGTGCCGCAGAAGTTGACAGAGGAA AGAAAAAGCTCCATGAATCTATCGAGGATGACGAAGATGTTATGGCTGAGTCTGATCGCCCTTTAAAGAGGCTGCGACGC AGAGGTGAAGGAGGCTCTGCTTTGGCAAGCCCTAGCTTGGGCAGTCCGACTTTAGAGGGACCTTCAATTAATGACGAAGA AAATGCTCCGATCCTATTGCCATATCATCCTGTACCAATTGAAAATGACCATGATGCTGGTGAGCTCATTCTTACAAAAG TCGAGCCCATTACAAATATGCCTCTCAGTTCTATTCCTGATTCAGTAGATAGAGGGGACTCTTCCATGCTTGAGATAGAC AAATCAAATGGACATGTGGAAGAGAAGGCTGGTGAAACAGTAAGTACTGCTGATGGTACTACCAATGACATTTCTCCTAC AACTGTTGCGAGATTTAGTGATCACAAGCTAGCTGCAACCATAGAAGAACCTCCAGCACTTGAATTAGCTTCTTCTGCTT CAGGCGAGGTGAAGATTAATCTTAGTTTTGCTCCTGCAACTGGAGGATCAAATCCCCATTTACCTTCTATGGAGGAACTA CGAAGAGCAATGGAGGAGAAGTGTCTCCGATCATACAAAATACTGGACCCCAACTTTTCTGTGCTTGGTTTTATGAACGA CATCTGTAGCTGCTACCTGGACCTGGCAACAAACGGGAGAGATTCAGCTAACCAATTGCCAAAAAACTTGCCGTTTGTCA CAACGAACATTGATGCATTGAAGAAATCTGCAGCAAGGATGGCTTATACTTCTCAGGCCAGTAATGATGTTGTTGAAATC TGCAGCAACGAACATATGCGTGATGCTGAAAACGGTGCAGTTGGTGATTCCATGGCTTTGGTAGTTGTTCCTGAATGCCA ACTTTCTGCAGATGAGTGGAGATTGATAAGCAGCGTCGGGGACATCAGTCTAGGGAAAGAAACTGTTGAGATTCCCTGGG TGAATGAGGTCAACGACAAAGTTCCTCCAGTTTTCCATTACATAGCCCAAAGTCTTGTGTATCAAGATGCTGCAGTGAAG TTTTCGCTTGGGAATATCAGGGATGACCAATGCTGCTCCTCTTGCTGTGGAGATTGCTTGGCTCCGTCAATGGCATGCAG ATGTGCAACTGCTTTTAATGGCTTTGCATACACAGTAGATGGCCTCCTACAGGAAGATTTTCTGGAACAGTGTATCTCTG AGGCCCGTGATCCGCGGAAACAAATGTTACTGTATTGCAAAGAATGTCCTTTAGAGAAAGCCAAAAAAGAAGTAATCCTG GAACCGTGCAAAGGGCATCTAAAGAGAAAGGCCATTAAAGAATGTTGGAGCAAGTGTGGCTGCATGAAGAATTGTGGCAA CCGAGTTGTCCAACAGGGCATTCACAACAAGCTGCAGGTGTTTTTCACGCCCAATGGGAGAGGCTGGGGACTCAGAACTC TAGAAAAACTGCCTAAAGGAGCATTTGTCTGTGAGCTTGCTGGAGAGATATTGACCATTCCAGAGTTGTTCCAACGCATC TCTGACAGGCCTACTTCTCCAGTAATATTGGATGCGTACTGGGGTTCTGAAGACATTTCAGGGGATGACAAAGCACTTTC TCTTGAAGGAACGCACTATGGAAATATTTCCAGGTTCATCAATCACAGATGCTTAGATGCAAATTTGATTGAGATCCCAG TTCATGCGGAGACTACTGATTCACACTACTATCATCTTGCATTCTTCACAACAAGAGAGATCGATGCTATGGAGGAACTT ACCTGGGATTATGGTGTCCCATTCAACCAAGATGTGTTTCCCACGAGTCCGTTCCATTGTCAATGTGGTAGCGACTTCTG CCGAGTCAGGAAGCAGATTAGCAAAGGCAAGAATGTGAAGAAGCGAGCATGAGAGAGAGCTTTACATTGGTAGGTTTTGA AGATATGTGGTGGGCGCAAACTCCTTTTTGTTGTGAATGAATTGGCAAAAAAATTCCAATCGAAAAACGCT ....N...........................................................i............... .....................................................................i.......... ..........................................................i..................... ...........N.....i........N............N........................................ ....................................................N.................N......... ...............................................N................................ ............N..............i........N....i...................................... ......................................................................N......... ..N.........................................N.....N..N.......................... .................i...............................................i.............. .......N.....N......................................N...........N............... ................................................................................ ....................................................................i........... .........i..............................................................N....... ................................................................................ ............N.........................i........................N..N............. ...............i......N............................N......................N..... ...........N.................................................................... ...N..............................................................i............. ......................i......N.......................................i....N..... N................i....................i......................................... .......................N...................N.................................... .........................................i.....................N................ .....................................................N.......................... ................................................................................ ...............................N................................N............... .................N..............................N.......N....................... ...N...........................................................N....i........... ..........i....................N..............................N................. ................................N...............N............................... ....N...............................N.................................. Pos Score Pred ------------------------ 5 0.392 - 65 0.560 Yes 150 0.614 Yes 219 0.684 Yes 252 0.476 - 258 0.553 Yes 267 0.420 - 280 0.227 - 373 0.324 - 391 0.175 - 448 0.203 - 493 0.160 - 508 0.529 Yes 517 0.349 - 522 0.841 Yes 631 0.399 - 643 0.233 - 685 0.351 - 691 0.269 - 694 0.369 - 738 0.863 Yes 786 0.801 Yes 808 0.206 - 814 0.292 - 853 0.078 - 865 0.200 - 1029 0.554 Yes 1050 0.780 Yes 1113 0.170 - 1213 0.307 - 1239 0.817 Yes 1264 0.135 - 1267 0.223 - 1296 0.661 Yes 1303 0.362 - 1332 0.465 - 1355 0.290 - 1372 0.124 - 1444 0.195 - 1507 0.790 Yes 1543 0.753 Yes 1550 0.237 - 1590 0.810 Yes 1595 0.138 - 1601 0.243 - 1618 0.662 Yes 1639 0.602 Yes 1704 0.329 - 1724 0.393 - 1802 0.690 Yes 1824 0.430 - 1894 0.385 - 2032 0.349 - 2065 0.210 - 2098 0.297 - 2129 0.361 - 2137 0.272 - 2164 0.259 - 2224 0.317 - 2229 0.680 Yes 2251 0.782 Yes 2272 0.444 - 2303 0.314 - 2353 0.460 - 2369 0.496 - 2405 0.142 - 2437 0.041 -

Explain the output -- Processing locus 89: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGTACTATTCCGATCTAGTTTCTGTAAAATCAATGGATAAGAGAATTAAAAAGATTCTCTTTTGGTTTTTTATGATCGA TTTATTAGTTCTTTTATGTAAGTGATCTTTTTGATTGAATTTTTGATTCTTTTGATGATGTTTACAGAATCCGATTCCAC AGATGGAACTATGTGCTGTGGATCAGACCGATTAAACCAGATCGTGTCATCAAGATCTTCGTTGCCAATTTCTTTCGAGG AAGATAACAATCTTGTTACCAACACAGACATGAATCACTTAACAGTCGAAACAGAGGATACGTTTGCGAGCTTGCTTGAG CTTGCAGCTAACAACGATGTTGAAGGTGTAAGGCTATCTATCGAGAGAGACCCTTCTTGTGTAGACGAAGCTGGTCTCTG GTACGGTCGTCAAAAAGGTTCTAAAGCTATGGTCAACGATTACAGGACTCCGTTGATGGTTGCTGCTACTTACGGAAGCA TTGATGTGATCAAGCTTATTGTTTCTTTGACTGATGCTGACGTGAACCGTGCTTGCGGGAATGATCAGACCACTGCGTTA CACTGCGCTGCTTCTGGAGGAGCTGTGAATGCTATCCAAGTTGTTAAGCTGCTTCTTGCAGCTGGAGCTGATTTGAATCT GTTGGATGCTGAAGGTCAACGAGCTGGTGATGTTATTGTTGTTCCTCCTAAGCTTGAAGGCGTGAAGCTGATGCTTCAGG AGCTTCTTTCTGCTGATGGATCATCTACTGCGGAGCGGAATCTACGGGTTGTGACAAATGTTCCGAATAGAAGCTCATCT CCGTGTCATTCTCCTACTGGAGAGAATGGTGGATCAGGGTCTGGTTCACCGCTCGGCTCTCCTTTTAAGCTGAAATCTAC TGAATTCAAGAAAGAGTATCCGGTTGATCCGTCTTTGCCAGATATCAAGAACAGTATCTACGCGACTGATGAGTTTAGAA TGTATTCCTTCAAGGTCCGGCCTTGCTCTCGTGCTTATTCACATGATTGGACTGAGTGTCCTTTTGTTCACCCGGGTGAA AACGCGAGGAGGAGAGACCCGAGGAAGTTCCATTACAGCTGCGTTCCTTGCCCGGATTTTAGGAAAGGAGCTTGTAGGAG AGGAGATATGTGTGAGTATGCGCACGGTGTGTTTGAATGCTGGCTTCATCCGGCTCAGTACAGGACCCGTCTTTGCAAAG ATGGAACAGGCTGTGCTCGGCGGGTTTGTTTCTTTGCGCATACACCCGAGGAGCTTCGACCTTTGTACGCATCAACTGGT TCAGCGGTTCCTTCGCCTAGATCGAATGCTGATTATGCAGCTGCTTTGAGTCTCCTTCCTGGTTCTCCATCAGGAGTCTC TGTCATGTCCCCGCTTTCCCCATCAGCAGCGGGGAACGGAATGTCTCATTCGAATATGGCTTGGCCACAACCAAATGTCC CTGCGTTGCACTTACCAGGAAGCAATCTACAGTCAAGCAGGCTAAGGTCTTCTCTCAATGCAAGGGATATCCCGACGGAT GAGTTCAATATGTTAGCGGATTACGAGCAGCAGCAACTCCTCAACGAGTATTCCAATGCTCTGAGCCGTTCTGGTCGGAT GAAATCAATGCCTCCTTCGAATCTTGAAGATCTTTTCTCAGCAGAAGGCTCTTCATCTCCCCGGTTCACTGATTCCGCTT TAGCTTCCGCGGTGTTCTCGCCTACACACAAGTCAGCTGTCTTCAACCAGTTCCAACAACAGCAACAGCAGCAGCAGAGC ATGTTGTCTCCAATCAACACAAGCTTTTCTTCACCAAAGAGCGTTGACCACTCATTGTTTTCAGGTGGAGGAAGAATGTC TCCTCGGAATGTTGTTGAACCAATATCACCCATGAGTGCTCGGGTTTCCATGTTGGCTCAGTGCGTGAAGCAACAACAAC AGCAACAGCAGCAGCAGCAGCAGCAACATCAGTTCCGTAGCCTTAGCTCCAGAGAGCTCAGAACAAACTCTAGCCCAATC GTTGGTTCACCGGTAAACAACAACACATGGTCATCAAAATGGGGATCTTCAAATGGTCAACCGGATTGGGGAATGAGCTC AGAAGCACTTGGTAAGTTGAGATCTTCGTCATCGTTTGATGGTGATGAGCCTGATGTGTCATGGGTCCAGTCACTGGTGA AGGAGACTCCAGCAGAAGCCAAAGAGAAAGCAGCAACATCTTCCTCAGGGGAACACGTGATGAAGCAGCCAAATCCGGTT GAACCGGTAATGGATCATGCTGGGCTAGAAGCTTGGATTGAGCAAATGCAGCTCGATCAGCTTGTGGCTCAGCAGAATTG AGAGACCTAACCAAAGAATCACATGTTCTTGTCTGGTCTCTCTATGTGGTAGATAAAAACCTCTTTTTTACAGGTGGTGG TGGTAGAAGGGTAACAAGAT .................................N......................................N....... ...............N......................................N..N...................... ..i.......N..................................................................... .............................i.................................................. ................N............................................................... ............................i..........................i........................ ...N.............................N..........................N................... ............................N................................................... .....N.......................N........................................N......... ...............N.........................................N...................... .........................N...................................................... ....................................................................i..........i ..........................................N..................................... ................................................................................ .......N.........N..................N........................................... i............................................................................... .........................N........N............................................. ....i...................................i..............i..................N..... .........................................................N....................N. .........N.............................................N......................i. .......i........................................................................ ................................................................................ i..........................................................................i.... ........N......................i.................N.............................. ................................................................................ ..........................N...........i.............i...................i....... ......................................N.....N........N......i................... ...........................................................N.................... .........i......N............................N.................................. ......................N....................N.................................... .................... Pos Score Pred ------------------------ 34 0.115 - 73 0.043 - 96 0.280 - 135 0.132 - 138 0.142 - 163 0.506 Yes 171 0.412 - 270 0.567 Yes 337 0.232 - 429 0.857 Yes 456 0.734 Yes 484 0.330 - 514 0.190 - 541 0.062 - 589 0.089 - 646 0.163 - 670 0.075 - 711 0.203 - 736 0.250 - 778 0.389 - 826 0.371 - 949 0.520 Yes 960 0.867 Yes 1003 0.279 - 1128 0.220 - 1138 0.408 - 1157 0.321 - 1201 0.610 Yes 1306 0.320 - 1315 0.172 - 1365 0.512 Yes 1401 0.627 Yes 1416 0.801 Yes 1435 0.382 - 1498 0.205 - 1519 0.384 - 1530 0.377 - 1576 0.392 - 1599 0.611 Yes 1608 0.754 Yes 1761 0.607 Yes 1836 0.748 Yes 1849 0.399 - 1872 0.532 Yes 1890 0.225 - 2027 0.443 - 2039 0.587 Yes 2053 0.612 Yes 2073 0.714 Yes 2119 0.386 - 2125 0.250 - 2134 0.181 - 2141 0.595 Yes 2220 0.473 - 2250 0.632 Yes 2257 0.228 - 2286 0.437 - 2343 0.111 - 2364 0.071 -

Explain the output -- Processing locus 90: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTATTGCTTTTTCTTTTCCGGTGAGGTCCCGTTGTTCTTTGTTATTTTTGATTTTGCCGGGATAGAAATAGAGTTTTCCG GTTGTTTTCCTTCTCGTCTAACGTCCCGAGCCGAGAATGAGTTCTTCGCAATCACCGTCGACCCCGAGCGCTTCTCTTGT CGATTCTAGCGATTCAAAACATCCAGACGATCTGCCTCAGATCTATAAACGGAGATCAGTTTGGACGTCTTCTGAAGATG CTGTTTCTTCATCGAATTCCCCCGAGCAGACGACTCCCTTCACGGTTCGGGAAGACACCAATGCCGATATCGCTCGGGAA TTGGATTTACCCGACGATCCCGAGCCGCATCTCGTTCGGAGGTCTTCTGCTCCTATGGTCGATGAAGCTGGGACATCCAA TTGGCAAGATGCGTCCGAGCCCCTCATGCCGACGGTTAAGATCGAGGACTTTCTTTACTTCGGCCCGAATGAGACAGAGG ATATCTTGCGTCTTAACGAGCAGAAGGCTTTCGAGAAAGCTGAGAAGAAGAAGAGGAAAAAGAAGAAAAAGGCGGTAATG CCAAATCCTCCCGGTTCATCAATGTGTACCGAGCAATCTCTCTCGGATCTTAAGGCTCGATTTGGTCTTGGTGCTGTAAC CTTGCGTGTTCCGAGCCCTGAAGAGCGAGCCGACAGTCCTCCTGCAGGTTTCTATACTCTGTACGAGGGATTCTTCTACG GCTGTCTCTTATGGTTGCCGATCCCGAGATTGGTCCTGGAATATGTGACATCATATCAGATCGCTCTTTCCCAGATCACG ATGCGGTCTTTGAGACATCTGCTCGGGATTTTGATTCGAAGTTACGAGTCTGAAACGGAGATAACGCTGGCTCATCTGAG AAATTTTCTCGAGATCCGGCGGGTGCCAAAATCCGAAGTGGACCGGTACTACATTTCCCCCGCTAAGGGAAAGAAGATTA TCGATGGGTTCCCGAGCAAAGACGAGCCATATACCGATCACTTCTTCTTTGTAGCCATTGAAGATGCTATTCATGAAGAT CTCCTCGGGACTGTTCTGACGAGATGGGGAATTTTGGAACGCACTCTCAAGTTTCTCGAGCCGATTCCAGACGATTTTCT TTCGGCTTTTCACGCGTTGTCGGCTCGAAAGTGCGATTGGCTGAAGCATTTTTCTCGGGAACGAGTTGAGTGTGCGCTTC GTCTTCTTCACGGCGTTTCTTGTCCCACTAGTTCGGAATCTTCCGATCATCGCACTCAATTTTTCGTGGACATGCAATCG ACGAAACTTACTTTGCGGGAGGTCTATGCCAAGAAGAAAGAGGACAAGGAGAGGCGATTGGCGGAAGAGAGGCGCCTTGT TAATACGGGATTGATCTCACCGCGGGCCGATCCTGAGGCGACCCAAGATGGGAATGTTATTCCCGATGCTACCGCGCCGC AGACTGCTCCCGAGGCATCTCGAGATGGGGTCAATCCCTATACTGCCGGGCCGGTTGACGCGGCGCCCGCTGAGGCTCAG GGAGCCGAGCCATCCGCTGCTGCGCCCGAGGCGGTTTTGGCGTTACCTGCGATTGACAAAGCGGCAGGTAAGCGCATTCG GGTTGACGATGTGTCGTCAAAGAAGAAGAAGAAGAAAAAGAAGGCATCCGGCTCCGAGGTGGAGAAAATACTCCCCATCT TTGAAGATCGTACTGCCTCGGCTAATCTGCTCGGGGGATGTGTTGGTCCCTTGCTTCCTCCTCCAGATACTCTTCTGGAA TCTCGGAAGTATGCCGAGACGGCATCTCACTTTCTGCGGGCTGTCGCTTCGATGAACCGGATGGTACATTCGTATGATTC GGCTATGCGAAGCAACATGGAGGTGGCTGGTAAATTGGCCGAGGCGGAGTCTCGGATTCAGGCCATCGAGCGAGAGAAGA ACGAGGCACTCTCCGAAGCTGCTGCAGCGAAACTGGAAAAGGAAGAGGTCGAGAGGACGGCTCATGTGAATAAAGAGAAC GCCATTAAGATGGCCGAGCAGAATCTCAAGGCGAACTCTGAGATCGTCCGTCTTAAGCGGATGTTATCCGAGGCGAGGGG ACTTCGTGACAGCGAGGTGGCTCGGGCCGTCCAGACGACGAGGCGGGAGGTCTCCGAGACTTTTATTGCTAAAATGAAGA ACGCCGAGCACAAAGTGTCGCTCCTTGATGAGGTCAATGACCGATTCATGTACTTGTCTCAGGCGCGAGCCAACGCGCAG CTGATCGAGGCCCTCGAAGGTGGCGGAGTTCTGGAGAGTGAGAAGGAGCAAGTTGATGAGTGGTTAAAAGATTTCGCCGA TGCTGAGGTAAATCTTAATCGCTTCATGTCCGAGCTGAAAGATGACCTCAAAGCTCCAGCTCCCGAGCCCGCTCCTCTGA GTCCGGGTGGTCACAGATCGGTCGAGTCATTGGCTGACGAGGCGGGAATTACAGATCAAGCCGGATCTCTCCTCCCTGCC AAGGATAATCGACCATCCGAAGATCTCGACTAGGGACCCGAGGCGGTTTTTTGTTTTTCTTCTCTCTGCTCGGTTATAAT ACTTATAGCTGGATGGCTTTATCCGTTATGTAGAACCTGCCTGGATGGCTTT ................................................................................ ....................................i........................................... .............................................................................N.. ............................................................N................... ......................................................i......N.................. ........N................i..........................................N........... .............................................................................N.. .....................i.......................................................... ................................................................................ ..........i...............................i..................................... N............................................................................... ................................................................................ ...N...........................................................N........N....... .......................i........................................................ ................................................................................ .......................................................................i........ .........................N...................................................... ...............................................i.....N...........N.............. ........................N....................................................... ................................................................................ ........N....................................................................... .....................................N.......................................... ..........N........................................i........i............N...... ....i...........i............................................................... ...............................................................N................ .........i..................................................N................... .........................................................................i...... ...........................N........N..........i................................ .......................................................N.......................N .........................i...............i...................................... ................................................................................ ................................................................................ ............N..............N................N....... Pos Score Pred ------------------------ 117 0.786 Yes 238 0.492 - 301 0.461 - 375 0.706 Yes 382 0.296 - 409 0.179 - 426 0.608 Yes 469 0.131 - 558 0.381 - 582 0.775 Yes 731 0.696 Yes 763 0.665 Yes 801 0.430 - 964 0.363 - 1024 0.160 - 1033 0.087 - 1064 0.779 Yes 1272 0.602 Yes 1306 0.362 - 1408 0.608 Yes 1414 0.111 - 1426 0.289 - 1465 0.277 - 1609 0.117 - 1718 0.484 - 1771 0.404 - 1812 0.639 Yes 1821 0.804 Yes 1834 0.265 - 1845 0.795 Yes 1857 0.877 Yes 1984 0.213 - 2010 0.866 Yes 2061 0.165 - 2154 0.716 Yes 2188 0.341 - 2197 0.361 - 2208 0.662 Yes 2296 0.256 - 2320 0.302 - 2346 0.754 Yes 2362 0.509 Yes 2573 0.045 - 2588 0.061 - 2605 0.108 -

Explain the output -- Processing locus 91: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACCATCGTTAATATCAAACGCGTTAGGTCGGTTTGACCGGTCTGGTTTGGTGAGCGCTGGTAATAATTTCACCATCCCA ATCAATCACAGACAAAATTGAAAACCCCAATTAATAATACATAAAATGAAAAACATGATGAATTATAAATTAAAACTCTG TTCTGTCTCAAAAAACTCAAAAGGAGTCTCTCTCTCACCTACACCACACCTAACCAAACCCCCTACGATTCACACAGAGA GAGATCTTCTTCTTCCTTCTTCTTCCTTCTTCTTTCTTCTTCTTTCTTCTTCTAGCTACAACATCTACAACGCCATGTCC TCTTCTTCTTCTTCGTCAACCTCCATGATCGATCTCATGGCAGCAATCATCAAAGGAGAGCCTGTAATTGTCTCCGACCC AGCTAATGCCTCCGCTTACGAGTCCGTAGCTGCTGAATTATCCTCTATGCTTATAGAGAATCGTCAATTCGCCATGATTG TTACCACTTCCATTGCTGTTCTTATTGGTTGCATCGTTATGCTCGTTTGGAGGAGATCCGGTTCTGGGAATTCAAAACGT GTCGAGCCTCTTAAGCCTTTGGTTATTAAGCCTCGTGAGGAAGAGATTGATGATGGGCGTAAGAAAGTTACCATCTTTTT CGGTACACAAACTGGTACTGCTGAAGGTTTTGCAAAGGCTTTAGGAGAAGAAGCTAAAGCAAGATATGAAAAGACCAGAT TCAAAATCGTTGATTTGGATGATTACGCGGCTGATGATGATGAGTATGAGGAGAAATTGAAGAAAGAGGATGTGGCTTTC TTCTTCTTAGCCACATATGGAGATGGTGAGCCTACCGACAATGCAGCGAGATTCTACAAATGGTTCACCGAGGGGAATGA CAGAGGAGAATGGCTTAAGAACTTGAAGTATGGAGTGTTTGGATTAGGAAACAGACAATATGAGCATTTTAATAAGGTTG CCAAAGTTGTAGATGACATTCTTGTCGAACAAGGTGCACAGCGTCTTGTACAAGTTGGTCTTGGAGATGATGACCAGTGT ATTGAAGATGACTTTACCGCTTGGCGAGAAGCATTGTGGCCCGAGCTTGATACAATACTGAGGGAAGAAGGGGATACAGC TGTTGCCACACCATACACTGCAGCTGTGTTAGAATACAGAGTTTCTATTCACGACTCTGAAGATGCCAAATTCAATGATA TAAACATGGCAAATGGGAATGGTTACACTGTGTTTGATGCTCAACATCCTTACAAAGCAAATGTCGCTGTTAAAAGGGAG CTTCATACTCCCGAGTCTGATCGTTCTTGTATCCATTTGGAATTTGACATTGCTGGAAGTGGACTTACGTATGAAACTGG AGATCATGTTGGTGTACTTTGTGATAACTTAAGTGAAACTGTAGATGAAGCTCTTAGATTGCTGGATATGTCACCTGATA CTTATTTCTCACTTCACGCTGAAAAAGAAGACGGCACACCAATCAGCAGCTCACTGCCTCCTCCCTTCCCACCTTGCAAC TTGAGAACAGCGCTTACACGATATGCATGTCTTTTGAGTTCTCCAAAGAAGTCTGCTTTAGTTGCGTTGGCTGCTCATGC ATCTGATCCTACCGAAGCAGAACGATTAAAACACCTTGCTTCACCTGCTGGAAAGGTTGATGAATATTCAAAGTGGGTAG TAGAGAGTCAAAGAAGTCTACTTGAGGTGATGGCCGAGTTTCCTTCAGCCAAGCCACCACTTGGTGTCTTCTTCGCTGGA GTTGCTCCAAGGTTGCAGCCTAGGTTCTATTCGATATCATCATCGCCCAAGATTGCTGAAACTAGAATTCACGTCACATG TGCACTGGTTTATGAGAAAATGCCAACTGGCAGGATTCATAAGGGAGTGTGTTCCACTTGGATGAAGAATGCTGTGCCTT ACGAGAAGAGTGAAAACTGTTCCTCGGCGCCGATATTTGTTAGGCAATCCAACTTCAAGCTTCCTTCTGATTCTAAGGTA CCGATCATCATGATCGGTCCAGGGACTGGATTAGCTCCATTCAGAGGATTCCTTCAGGAAAGACTAGCGTTGGTAGAATC TGGTGTTGAACTTGGGCCATCAGTTTTGTTCTTTGGATGCAGAAACCGTAGAATGGATTTCATCTACGAGGAAGAGCTCC AGCGATTTGTTGAGAGTGGTGCTCTCGCAGAGCTAAGTGTCGCCTTCTCTCGTGAAGGACCCACCAAAGAATACGTACAG CACAAGATGATGGACAAGGCTTCTGATATCTGGAATATGATCTCTCAAGGAGCTTATTTATATGTTTGTGGTGACGCCAA AGGCATGGCAAGAGATGTTCACAGATCTCTCCACACAATAGCTCAAGAACAGGGGTCAATGGATTCAACTAAAGCAGAGG GCTTCGTGAAGAATCTGCAAACGAGTGGAAGATATCTTAGAGATGTATGGTAACGAAACTATTGAAGCCACACACTCACT GTGTACTTATATTTATATATATACGGCACAGAAATTGCCACATTATGATGATCATTAAGTTTGTGATCGCAA ................................................................................ .............................................i........i..i...................... ................................................................................ ..........................................................................i..... ........................i...........i........................................... .....N........................................i..........................i...... ......................................i......................................... .................................................N..i........................... .................................................................N.............. ..................N..............N..N..N.....N.......................N.......... ................N.....N.................N..................N................N... .........N...................N.............................N.................... ............N.....................................................N..N.......... .......N........................................................................ ..............................................................N...........N..... .....i......N.....N.................N.......................N................... ......................................................................N......... .....N......................................N......................i............ ................................................................................ ......................N...N.................................................N... ...........................................................N.................... .............................i.................................................. .............................................................................i.. ...........N.......i.........................................N......N........... ................................................................................ .........i...................................................................... ....................................N...............i........................... ................................................................................ ......i..i..........................i........................i.................. ....i.........N...........................................i..................... ..........................................i...N................................. ............................................N..N........................ Pos Score Pred ------------------------ 126 0.508 Yes 135 0.687 Yes 138 0.780 Yes 315 0.879 Yes 345 0.778 Yes 357 0.795 Yes 406 0.148 - 447 0.590 Yes 474 0.643 Yes 519 0.592 Yes 610 0.351 - 613 0.666 Yes 706 0.107 - 739 0.319 - 754 0.160 - 757 0.112 - 760 0.141 - 766 0.189 - 790 0.308 - 817 0.307 - 823 0.258 - 841 0.258 - 860 0.293 - 877 0.316 - 890 0.325 - 910 0.432 - 940 0.245 - 973 0.314 - 1027 0.330 - 1030 0.494 - 1048 0.199 - 1183 0.270 - 1195 0.114 - 1206 0.784 Yes 1213 0.427 - 1219 0.264 - 1237 0.114 - 1261 0.429 - 1351 0.192 - 1366 0.217 - 1405 0.148 - 1428 0.597 Yes 1543 0.413 - 1547 0.249 - 1597 0.271 - 1660 0.269 - 1710 0.641 Yes 1838 0.734 Yes 1852 0.200 - 1860 0.552 Yes 1902 0.375 - 1909 0.366 - 2010 0.578 Yes 2117 0.151 - 2133 0.662 Yes 2247 0.723 Yes 2250 0.791 Yes 2277 0.519 Yes 2302 0.505 Yes 2325 0.773 Yes 2335 0.179 - 2379 0.705 Yes 2443 0.587 Yes 2447 0.122 - 2525 0.042 - 2528 0.063 -

Explain the output -- Processing locus 92: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACCATCGTTAATATCAAACGCGTTAGGTCGGTTTGACCGGTCTGGTTTGGTGAGCGCTGGTAATAATTTCACCATCCCA ATCAATCACAGACAAAATTGAAAACCCCAATTAATAATACATAAAATGAAAAACATGATGAATTATAAATTAAAACTCTG TTCTGTCTCAAAAAACTCAAAAGGAGTCTCTCTCTCACCTACACCACACCTAACCAAACCCCCTACGATTCACACAGAGA GAGATCTTCTTCTTCCTTCTTCTTCCTTCTTCTTTCTTCTTCTTTCTTCTTCTAGCTACAACATCTACAACGCCATGTCC TCTTCTTCTTCTTCGTCAACCTCCATGATCGATCTCATGGCAGCAATCATCAAAGGAGAGCCTGTAATTGTCTCCGACCC AGCTAATGCCTCCGCTTACGAGTCCGTAGCTGCTGAATTATCCTCTATGCTTATAGAGAATCGTCAATTCGCCATGATTG TTACCACTTCCATTGCTGTTCTTATTGGTTGCATCGTTATGCTCGTTTGGAGGAGATCCGGTTCTGGGAATTCAAAACGT GTCGAGCCTCTTAAGCCTTTGGTTATTAAGCCTCGTGAGGAAGAGATTGATGATGGGCGTAAGAAAGTTACCATCTTTTT CGGTACACAAACTGGTACTGCTGAAGGTTTTGCAAAGGCTTTAGGAGAAGAAGCTAAAGCAAGATATGAAAAGACCAGAT TCAAAATCGTTGATTTGGATGATTACGCGGCTGATGATGATGAGTATGAGGAGAAATTGAAGAAAGAGGATGTGGCTTTC TTCTTCTTAGCCACATATGGAGATGGTGAGCCTACCGACAATGCAGCGAGATTCTACAAATGGTTCACCGAGGGGAATGA CAGAGGAGAATGGCTTAAGAACTTGAAGTATGGAGTGTTTGGATTAGGAAACAGACAATATGAGCATTTTAATAAGGTTG CCAAAGTTGTAGATGACATTCTTGTCGAACAAGGTGCACAGCGTCTTGTACAAGTTGGTCTTGGAGATGATGACCAGTGT ATTGAAGATGACTTTACCGCTTGGCGAGAAGCATTGTGGCCCGAGCTTGATACAATACTGAGGGAAGAAGGGGATACAGC TGTTGCCACACCATACACTGCAGCTGTGTTAGAATACAGAGTTTCTATTCACGACTCTGAAGATGCCAAATTCAATGATA TAAACATGGCAAATGGGAATGGTTACACTGTGTTTGATGCTCAACATCCTTACAAAGCAAATGTCGCTGTTAAAAGGGAG CTTCATACTCCCGAGTCTGATCGTTCTTGTATCCATTTGGAATTTGACATTGCTGGAAGTGGACTTACGTATGAAACTGG AGATCATGTTGGTGTACTTTGTGATAACTTAAGTGAAACTGTAGATGAAGCTCTTAGATTGCTGGATATGTCACCTGATA CTTATTTCTCACTTCACGCTGAAAAAGAAGACGGCACACCAATCAGCAGCTCACTGCCTCCTCCCTTCCCACCTTGCAAC TTGAGAACAGCGCTTACACGATATGCATGTCTTTTGAGTTCTCCAAAGAAGTCTGCTTTAGTTGCGTTGGCTGCTCATGC ATCTGATCCTACCGAAGCAGAACGATTAAAACACCTTGCTTCACCTGCTGGAAAGGATGAATATTCAAAGTGGGTAGTAG AGAGTCAAAGAAGTCTACTTGAGGTGATGGCCGAGTTTCCTTCAGCCAAGCCACCACTTGGTGTCTTCTTCGCTGGAGTT GCTCCAAGGTTGCAGCCTAGGTTCTATTCGATATCATCATCGCCCAAGATTGCTGAAACTAGAATTCACGTCACATGTGC ACTGGTTTATGAGAAAATGCCAACTGGCAGGATTCATAAGGGAGTGTGTTCCACTTGGATGAAGAATGCTGTGCCTTACG AGAAGAGTGAAAACTGTTCCTCGGCGCCGATATTTGTTAGGCAATCCAACTTCAAGCTTCCTTCTGATTCTAAGGTACCG ATCATCATGATCGGTCCAGGGACTGGATTAGCTCCATTCAGAGGATTCCTTCAGGAAAGACTAGCGTTGGTAGAATCTGG TGTTGAACTTGGGCCATCAGTTTTGTTCTTTGGATGCAGAAACCGTAGAATGGATTTCATCTACGAGGAAGAGCTCCAGC GATTTGTTGAGAGTGGTGCTCTCGCAGAGCTAAGTGTCGCCTTCTCTCGTGAAGGACCCACCAAAGAATACGTACAGCAC AAGATGATGGACAAGGCTTCTGATATCTGGAATATGATCTCTCAAGGAGCTTATTTATATGTTTGTGGTGACGCCAAAGG CATGGCAAGAGATGTTCACAGATCTCTCCACACAATAGCTCAAGAACAGGGGTCAATGGATTCAACTAAAGCAGAGGGCT TCGTGAAGAATCTGCAAACGAGTGGAAGATATCTTAGAGATGTATGGTAACGAAACTATTGAAGCCACACACTCACTGTG TACTTATATTTATATATATACGGCACAGAAATTGCCACATTATGATGATCATTAAGTTTGTGATCGCAA ................................................................................ .............................................i........i..i...................... ................................................................................ ..........................................................................i..... ........................i...........i........................................... .....N........................................i..........................i...... ......................................i......................................... .................................................N..i........................... .................................................................N.............. ..................N..............N..N..N.....N.......................N.......... ................N.....N.................N..................N................N... .........N...................N.............................N.................... ............N.....................................................N..N.......... .......N........................................................................ ..............................................................N...........N..... .....i......N.....N.................N.......................N................... ......................................................................N......... .....N......................................N......................i............ ................................................................................ ......................N...N.................................................N... ........................................................N....................... ..........................i..................................................... ..........................................................................i..... ........N.......i.........................................N......N.............. ................................................................................ ......i......................................................................... .................................N...............i.............................. ................................................................................ ...i..i..........................i........................i..................... .......................................i...N.................................... .........................................N..N........................ Pos Score Pred ------------------------ 126 0.508 Yes 135 0.687 Yes 138 0.780 Yes 315 0.879 Yes 345 0.778 Yes 357 0.795 Yes 406 0.148 - 447 0.590 Yes 474 0.643 Yes 519 0.592 Yes 610 0.351 - 613 0.666 Yes 706 0.107 - 739 0.319 - 754 0.160 - 757 0.112 - 760 0.141 - 766 0.189 - 790 0.308 - 817 0.307 - 823 0.258 - 841 0.258 - 860 0.293 - 877 0.316 - 890 0.325 - 910 0.432 - 940 0.245 - 973 0.314 - 1027 0.330 - 1030 0.494 - 1048 0.199 - 1183 0.270 - 1195 0.114 - 1206 0.784 Yes 1213 0.427 - 1219 0.264 - 1237 0.114 - 1261 0.429 - 1351 0.192 - 1366 0.217 - 1405 0.148 - 1428 0.597 Yes 1543 0.413 - 1547 0.249 - 1597 0.278 - 1657 0.262 - 1707 0.708 Yes 1835 0.734 Yes 1849 0.200 - 1857 0.552 Yes 1899 0.375 - 1906 0.366 - 2007 0.578 Yes 2114 0.151 - 2130 0.662 Yes 2244 0.723 Yes 2247 0.791 Yes 2274 0.519 Yes 2299 0.505 Yes 2322 0.773 Yes 2332 0.179 - 2376 0.705 Yes 2440 0.587 Yes 2444 0.122 - 2522 0.042 - 2525 0.063 -

Explain the output -- Processing locus 93: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAACAGTAATTTGTTTCTAATCTAATTATTTGGTTTTGTTCAATAATCCTAGCAAAAATTCACGAAAGAGTTAATAATTT TGTAACCGGAATCATAGATAGGCCGGTTTATCCAAACCGGATATGACCTGTCCTCTATTAACAAACTGGTCGCGAGTCTC TAGGCAACTAAAACGGACACGTAATATGATAATAAGGCGTGGACTCGTATCTCTCGGCACTCACTTTCTCGCGACTCGTA GTCGTCTTCTCCACTTCGATACTCCTCTCTCTTGCCTCGTTCCTCTTGTTCCTCGCCGCCGCTCTATTCTACGGCTGTCT TCTTCTTTTTCCTCTTCCTCTGAAACCGGAGCTGCGTCAGTGCGGTTGACATCACCGAGAGACATGGCGGAATCGAGATC CCCTCCTGTAGCGAAGAAGGTGGAGCATGTGATGGAGATGTTCGGTGACGTTAGGGTTGACAATTACTACTGGCTCCGTG ACGATTCTCGCACCAATCCCGACATGCTTTCTTATCTTCGTGAAGAAAATCACTACACTGATTTCGTCATGTCTGGGACC AAGCAATTTGAGAATCAGCTGTTTGCTGAAATAAGAGGGAGGATCAAAGAAGATGATATATCTGCACCTCTACGCAAGGG TCCTTACTATTATTATGAGAAAAATCTTCAAGGAAAGGAATATATTCAGCACTGTAGACGTTTGATCACTGACAACAAAG CTGAGCCTTCTGTCTATGATACTATGCCAACTGGTCCAGATGCTCCTCCTGAGCATGTGATATTGGATGAGAATACCAAG GCTCAAGAGCATGACTACTACAGAATTGGGGCCTTCAAGGCGAGTCCCGATCATAAATTGGTGGCATATGCAGAGGACAC AAAAGGTGATGAGATATATACAGTGAATGTCATCGACTCTGAAGCCCTAAAACCAGTGGGGCAACAGCTTAAAGGACTTA CTTCTTATCTTGAATGGGCTGGTAATGATGCTTTGCTCTACATAACAATGGACGAAATCCTGCGACCTGATAAGGTTTGG TTACATAAATTAGGGACAGAGCAGAGCAGTGATGTATGCCTTTACCATGAGAAGGATGATATGTTTTCTCTTGAACTCCA CGCTTCTGAGAGCCATAAGTATTTATTTGTTGCATCTGAAAGCAAAACTACAAGATTTGTCTTCTCGCTTGATGTGTCCA AGACTCAGGATGGACTCAGGGTGTTGACACCTCGTGTAGATGGAATTGATTCATCTGTCAGTCATCGTGGGAACCACTTT TTCATCCAGAGAAGGAGCACTGAATTTTACAACTCAGAACTGATCGCCTGTCCAGTTGATGACACATCAAAGACAACTGT TCTGCTTCCACATAGAGAAAGTGTAAAGATTCAGGAAATTCAGCTTTTCCGAGATCATCTTGCAGTCTTCGAGCGTGAAA ACGGACTGCAGAAGATCACAGTTCATAGGCTTCCTGCTGAGGGACAGCCACTGGAAGGTCTCCAAGGTGGTCGTAATGTG AGCTTTGTTGACCCGGTCTATTCAGTTGACTCAACCGAGTCCGAATTTTCGTCCAGGGTTTTAAGGTTTAAATACTGCTC AATGAAAACACCTCCTTCTGTGTATGACTATGACATGGACAGTGGCACTTCCGTTGTCAAGAAGATTGACACAGTATTGG GAGGTTTTGATGCATCAAATTATGTTACAGAAAGAAAGTGGGTCGCTGCATCAGACGGAACTCAAATTCCAATGTCTATT GTGTATAACAAAAAACTTGCAAAGCTTGATGGATCTGATCCATTACTCCTTTATGGATATGGTTCATATGAGATTTCTGT GGATCCTTATTTCAAGGCATCAAGGTTGTCCCTGTTAGATCGTGGGTTTACCTTTGTAATTGCTCATGTTCGTGGGGGCG GTGAGATGGGGAGACAGTGGTATGAGAATGGGAAGCTGTTGAAGAAGAAAAACACATTCACTGATTTTATTGCTTGTGCT GAGCGTTTAATAGAACTAAAATATTGCTCGAAAGAAAAACTGTGCATGGAAGGGAGAAGTGCTGGTGGATTGCTGATGGG TGCTGTTGTTAATATGAGGCCTGACTTGTTCAAGGTGGTTATCGCTGGAGTTCCTTTTGTAGATGTCTTGACAACAATGC TGGACCCAACAATTCCGCTTACGACATCAGAGTGGGAGGAGTGGGGTGACCCCAGAAAGGAAGAATTTTACTTCTATATG AAGTCATATTCCCCTGTAGACAATGTAACGGCGCAGAATTATCCAAATATGCTTGTAACTGCTGGGTTAAATGATCCAAG AGTTATGTACTCTGAACCTGGCAAGTGGGTGGCAAAACTGAGAGAAATGAAGACCGACAACAACGTACTTCTGTTCAAAT GCGAGCTTGGCGCAGGGCATTTCTCAAAATCTGGGAGATTCGAGAAGCTTCAAGAAGATGCATTTACGTTTGCATTCATG ATGAAAGTTTTGGACATGATTCCTGCTTCTGGGTGAGTCCTCAAATTTAGCTACGTGATGATCAATTTATGATCTTCTTA CGTTGTTGAGATGAGAAGTCTTTACAGTTTAGGTTTGAAGTTGTTTTCGTCCTTT ................................................................................ ..........................................N..................................... .........................N...................................................... ................................................................................ ...............................................................i................ ..........................N....i.....N.......................................... .......................N............................................i........... ....................................................N........................... ..............N................................................................. ...............N.......i...............N..............N...........N............. ..........N........................................................N............ ........N.................N..................................................... .............N..........N..N...................i................................ ...............................N...N..........N........N....i................... .......................................................................N........ .........i.............................N........................................ ..........................................................N..................... ................................................................................ ...........................................................................N.... ................................................................................ .........N...........N.................................................N........ ............................N.......................N.....N........N............ .................................................................N.............. .....N...............N.....N.................................................... .............................................i.............................i.... .............N................................................N.............i... .............................................................................N.. ......................N.........................i.....................i......... ....N.........................................i...............................i. .........................................................N...................N.. i..............N.........................................N..........N........... ..........N............................................ Pos Score Pred ------------------------ 123 0.407 - 186 0.480 - 384 0.740 Yes 427 0.307 - 432 0.641 Yes 438 0.356 - 504 0.357 - 549 0.695 Yes 613 0.333 - 655 0.132 - 736 0.205 - 744 0.645 Yes 760 0.140 - 775 0.283 - 787 0.111 - 811 0.400 - 868 0.137 - 889 0.248 - 907 0.150 - 974 0.406 - 985 0.180 - 988 0.191 - 1008 0.619 Yes 1072 0.175 - 1076 0.240 - 1087 0.178 - 1096 0.176 - 1101 0.706 Yes 1192 0.335 - 1210 0.538 Yes 1240 0.207 - 1339 0.231 - 1516 0.199 - 1602 0.732 Yes 1624 0.258 - 1630 0.493 - 1635 0.809 Yes 1690 0.075 - 1702 0.238 - 1752 0.455 - 1789 0.205 - 1813 0.221 - 1819 0.413 - 1828 0.302 - 1906 0.201 - 1926 0.294 - 1942 0.133 - 1948 0.300 - 2046 0.569 Yes 2076 0.717 Yes 2094 0.331 - 2143 0.188 - 2157 0.596 Yes 2238 0.427 - 2263 0.309 - 2289 0.630 Yes 2311 0.521 Yes 2325 0.348 - 2367 0.594 Yes 2399 0.516 Yes 2458 0.077 - 2478 0.217 - 2481 0.681 Yes 2496 0.156 - 2538 0.043 - 2549 0.082 - 2571 0.053 -

Explain the output -- Processing locus 94: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAAAATCTTTTTGACCAAAAATAACAAAAAAAATGGAAGAAAATAATAAATTCCCTCTGAGTGGAAGGAGGGAAATTT GGCTCCCAAATGAAAAACTAAATTTCAAAACCCCGCCAAATAGGCTTTGGTATTATAAAGAAAACGCTCTCTCCACTCCT GATGATAATCTCAAAATCGTTCAAAGCACCGCTCAAATTCTCCGTCAAATCTTCTACTGCGCCGGTGATATCAAACCATC CGCCAATGGAGAACCATCCCAAGCGCCAGCGAACAACACGCCTCGCCGCCAGAAACTTGAAGCGAAAACTCAGTCACAAC ACAGATGGAGCGCCAATCGTCACACAACTTATTGATATTGACGACGAACCCATAGATCTCGTCGTCGCGATTCGCCGTCA CGTCGAGGTTCTTAACTCCAGCTTCTCAGATCCTGATTTTGATCACGAAGCTGTCAAAGAAGCTGCTGCTGATATCGCCG ATTTGGCTAAAATCGATGAAAATGTTGAAATCATCGTGGAAAATGGAGCGATCCCTGCTTTGGTGAGATATCTGGAATCC CCTTTGGTAGTTTGTGGCAATGTTCCCAAATCTTGCGAGCACAAGCTAGAGAAAGATTGTGCTCTTGCTCTTGGACTTAT TGCTGCGATTCAGCCTGGTTACCAGCAGCTTATTGTGGATGCTGGAGCCATAGTTCCCACCGTGAAGTTGTTGAAGAGAC GTGGGGAATGTGGTGAATGCATGTTTGCTAATGCTGTTATTAGGAGAGCCGCTGATATAATTACTAACATTGCTCATGAC AATCCAAGGATTAAAACAAATATAAGGGTTGAAGGTGGCATTGCACCACTTGTTGAGCTTCTCAATTTTCCGGATGTTAA AGTACAGAGGGCTGCGGCAGGGGCCTTGCGAACAGTTTCATTTAGAAACGACGAGAACAAGAGTCAAATTGTGGAGTTGA ATGCTTTACCCACACTTGTATTGATGCTTCAATCACAAGATTCTACCGTTCACGGGGAAGCGATTGGGGCAATTGGAAAT CTTGTCCACTCATCTCCTGACATCAAGAAAGAGGTTATCCGTGCTGGTGCCTTACAACCAGTAATTGGTCTACTTAGCTC CACTTGTTTGGAAACACAGAGAGAAGCAGCATTATTAATAGGTCAATTTGCTGCGCCTGATTCAGATTGCAAGGTGCACA TTGCCCAGAGAGGTGCCATTACACCACTGATTAAGATGCTTGAATCATCAGATGAGCAGGTCGTGGAAATGTCAGCTTTT GCTCTTGGTCGTTTGGCTCAGGACGCACACAATCAAGCTGGCATTGCACATAGAGGAGGCATCATCTCACTACTAAATCT TCTTGATGTGAAAACGGGGTCTGTGCAACACAATGCTGCCTTTGCTTTGTATGGCCTTGCAGATAACGAGGAAAACGTAG CAGATTTCATAAAGGCTGGAGGTATTCAAAAGCTTCAAGATGACAACTTTACTGTCCAACCGACTAGAGATTGTGTGGTT CGGACATTGAAGAGGCTGCAGAACAAGATTCACGGACCTGTACTAAACCAATTATTGTACCTAATGAGAACCGCTGAGAA GACAGTACAAATACGAATTGCTCTGGCTCTTGCACATCTTTGTGACCCTAAAGATGGAAAATTAATCTTTATTGATAACA ATGGAGTAGAGTTTTTGTTGGAACTTCTCTATTTCTCAAGCAATAAGCAGCAGAGATATAGTTCAAGTGCTTTATACGAG TTAGCTAAAAAAGCTACATCCTTTGCACCAGAGGATTCAGCTCCTTGTTCACCCACACAACAGGTGTTTCTGGGTGAAAA ATTCGTGAACAACCCCACTATGTCCGATGTCACATTCCTAATTGATGGTAAACAATTCTATGCACACAAGATTGGCTTGG TAGCTTCCTCTGATATATTTCGTGCAATGTTTGATGGTCTTTACAAGGAACGCAATGCTCAAAATGTGGAGATACCAAAT ATAAGATGGGAAGTGTTTGAGCTTATGATGAAGTTCATTTACTCAGGAAGAATTAACATCGCCAAACATTTGGCAAAAGA TCTGCTTGTAGCAGCTGATCAATATCTTCTCGAAGGTCTCAAACGTCAATGTGAATACACCATTGCACAGGAAATCTGTC TGGATAACATACCAGAGATGTATGAGTTAGCAGATACATTCAATGCTTCAGCCTTAAGAAGAGCTTGCACGCTCTTTGTT CTTGAGCATTTCACTAAGCTCAGCTCTCAATTATGGTTTGCAAAGTTTGTCAAGCAAATTATACCTGAAATTCGGAGTTA CATGACTGATATTCTCACACGGCCGGTCGAAGCTAGTCCCCCAACCGTCGTATAAAACATATAGGATTCCGGTTTGAAGT AGTTGATTTACTTTACTTAGGTAAAATTGTTTTTCTTTTGATAACCGGTCTGTATTTTGTTACTGCTATATCTC ..................................i............................................. .........N...................................................................... .....i.......................................................................... ....N........................................................................... ................................................................................ ...............N.....N....................N..................................... ...................N............................................................ ......................................N......................................... .......N........N...i.........N............................................N.... .........................................................................N...... ................................................................................ N......................i........................................................ ................................................................................ ................................................................................ ...................................i...............N................i........... ................................................................................ .....N..........................N.................N............................. .......................................N........................................ ...............................................................i................ .....................................................N.......................... N............................................................................... ................................................................................ ...................i......N.................N..............N.................... ..........................N......N....................N........N................ .....i..................N..N.................................................... ................................................N............................... .................i...N....................N..................................... ................................i............................................... .......................................................................... Pos Score Pred ------------------------ 35 0.663 Yes 90 0.282 - 162 0.466 - 246 0.538 Yes 325 0.327 - 496 0.152 - 502 0.279 - 523 0.383 - 580 0.177 - 679 0.068 - 728 0.280 - 737 0.198 - 741 0.696 Yes 751 0.107 - 796 0.097 - 874 0.113 - 961 0.086 - 984 0.703 Yes 1236 0.675 Yes 1252 0.448 - 1269 0.603 Yes 1366 0.216 - 1393 0.370 - 1411 0.109 - 1480 0.320 - 1584 0.617 Yes 1654 0.433 - 1681 0.487 - 1860 0.631 Yes 1867 0.288 - 1885 0.198 - 1900 0.294 - 1947 0.451 - 1954 0.222 - 1975 0.071 - 1984 0.341 - 2006 0.571 Yes 2025 0.356 - 2028 0.488 - 2129 0.395 - 2178 0.582 Yes 2182 0.185 - 2203 0.153 - 2273 0.640 Yes 2322 0.357 -

Explain the output -- Processing locus 95: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACCTCACTTTTCAGTACAAAAACAGAGTCGACAATTCTTGAAAACTCTGTTTGACATCTGTTTATTTTGACGGTCGAT TCACAGGTGGACCAATTCTAGTGGATAAAACTAGTGCCACGTAGTATGAATAAATACTATATAATAATACTTAAAATGAC AAGATATAATAACAAAGCCCCACCATCACCTGCTGTCCTGCGCGGCGTCTCCGTCGTCTCTGTCGCCGCATCTCCGTCAT CATTTTCGCTACCGCATAATCTCTGTCTCCGCCGTCACTGCCGCAGCCTAGAATATGATTTCTCGTTTCTGAAACCCTTG TATAAGGTAGAAAGAGAGAGAAATCATCCAAAGCAAGGACCTTCCAGCGACTTTCAGGGTTTAAGGTTTAGCTTCTCGAC TATGGCGATTCTAGGGCTTATCAAGAGGGTTACACGAATAACCGTAAACAATTCGAGGGTCAGAGTGTACCCAGTGAGGT ACTTCCAAAGGAAGGACCTTTCAAGCACAAATCTATTCAATGGTGAAGATGCTGCTAAGCTTCCTGTTCTGATCGTCGGC GCTGGACCGGTGGGACTCGTTTTATCCATCCTTCTCACCAAACTAGGAGTGAAATGTGCTGTTGTTGACAAAGCCACAAG CTTCTCGAAACATCCACAAGCACACTTTATCAACAACCGATCAATGGAGATCTTCCGTGAATTGGATGGATTAGCTGAGG AGATTGAAAGATCACAGCCTCCTGTAGATTTGTGGAGAAAATTTATTTACTGTACATCTCTCTCCGGCTCAACACTAGGC ACAGTTGATCACATGCAGCCTCAAGATTTTGAGAAAGTTGTCAGCCCGGCTTCTGTTGCTCACTTCTCCCAGTACAAATT GACAAATTTATTGCTTAAGCGGTTGGAAGACCTCGGCTTTCATGTTCGTGGTTCCAAAGAGTCGGATGGTCTTGAGGCTG ACTCTGTGGTTGCGAGGCAAATTCTTATGGGTCATGAGTGTGTGGGGATTGATGCTAACAAGGACAGTATTACTGCAACT GTTTCTTTTCTCAAAGGAGGCAAGCATATGAAAAGAAACATTCAATGTAGTCTACTGGTTGGTGCTGATGGTGCTGGGAG TGCAGTAAGAAAGCTCACAGTAATAGAAATGCGAGGGGAAAGAGATCTACAGAAGCTTGTTAGTGTCCATTTCATGAGTA GAGAGCTCGGTGAGTACCTGATCAGTAACAGACCAGGGATGCTATTTTTTATTTTTAACACCGATGGTATAGGAGTTCTT GTTGCCCATGATCTTCTACAAGGAGAATTTGTTTTGCAGATTCCATACTATCCACCTCAACAGAGTCTATCTGATTTTAG TCCTGAGATGTGCAAGATGCTGATATTCAATTTGGTGGGACATGAGCTTTCAGACTTGGATGTAGCTGATATTAAGCCAT GGGTCATGCATGCTGAAGTCGCCGAGAAATTCATGTGTTGTGAGAACAGAGTGATACTTGCTGGTGATGCTGCTCACCGG TTCCCGCCTGCTGGAGGTTTTGGAATGAACACTGGAATTCAGGATGCTCATAATCTTGCATGGAAAATAGCAGCTCTTGT TCAGGGTTCTGCAAACTCTTCAATTCTTAAAACTTATGAAACAGAACGTAGGCCGATTGCCCTTTCCAATACTTCACTCA GTGTTCAAAACTTCAGAGCAGCTATGTCAGTTCCTTCAGCGCTAGGCCTTGACCCAACAGTTGCAAACTCAGTGCATAGG TTTATAAACAAAACAGTTGGTTCGATCCTTCCAACTGGGCTGCAAAAGGCAATCTTGGATAATGTCTTTGCACTAGGTCG TGCACAGCTTTCAGAATCTCTGTTGAATGAGAGCAATCCACTGGGAAATCAGAGACTTAGCAGACTAAAGAGTATCTTCG AAGGAGGAAAAAGCCTTCAACTACAGTTTCCTGCAGAAGATCTTGGTTTCAGGTATTTAGAGGGAGCCATTGTTCCTGAT AACGAGTCTGAAGCTGGTGATCCCGAAGTACCGAGTGGTCGTAGAAGAGACTATGTCCCTTGTGCTGAACCAGGTTCAAG ACTGCCTCATATGTATGTGAAAATACTGTCAGATTCCACAAGAGAGGTTATTGTTTCTACACTGGATCTTGTTTCGACAG AGAAAGTGGAGTTTCTACTTATAATATCGCCATTACAAGAGTCCTATGAGCTAGCTCATGCTACATTCAAAGTCGCAAAA GAGTTTATGGCTAGTGTAAAAGTATGCGTGGTCTGGCCTAGTAGTGATGATGGTCTTGAAAGGAAGAGCAATTCAGCATT AGCTCCATGGGAAAATTATGTCGATGTTATGGAAGTTAAAAAACAAAATGGTGAAGGAACTTCGTGGTGGAGTATTTGTA AAATGTCAGAGAGAGGATCTATTTTAGTCCGTCCCGACCAACACATTGCTTGGCGAGCAAAGTCTGGTATTACTTTGGAT CCTACTCTGCACATGAGAGATGTCTTCACCATCATACTAGGGAAACAATGATGAACATCACAATTTTATTCCACAAATTC CTTTGTATACTACTTATTTTCAAATAAAATCACGTTATTTTTGTAATGGTGTTTCACGATTTCTTTACAT ................................................................................ .............................................i.............................i.... ................................................................................ ......................................................i......................... ................................................................................ .......................................N........N............................... .....................................................N.......................... ...........................................N.....................N.............. ................................................................................ ............N................................................................... .........................................N.......................N.............. ..........................i......N.................N............................ ...........................N................N......................N............ ............................N............................................N...... ......................................N........................N................ .......N........................................................................ .......i........i........................N.................N..................N. .....N...N......................N.................................N............. ........................i..................N...............i.................... ...................................N............................................ .......................i........................................................ .............................................................i.................. ..........................N..................................................... ................................................................................ ....................................................N........................... ..........i...N................................................................. .............................................N...........N...................... ......i................N......................N..N.............................. ......N..........N.....N....N..................N................................ ..i............................................................................. ............N......N...........................N..N............................. .............................................N........................ Pos Score Pred ------------------------ 126 0.654 Yes 156 0.695 Yes 295 0.617 Yes 402 0.724 Yes 520 0.282 - 529 0.165 - 614 0.455 - 684 0.480 - 706 0.423 - 813 0.459 - 922 0.150 - 946 0.086 - 987 0.616 Yes 994 0.255 - 1012 0.104 - 1068 0.487 - 1085 0.376 - 1108 0.241 - 1149 0.340 - 1194 0.283 - 1239 0.447 - 1264 0.168 - 1288 0.238 - 1368 0.647 Yes 1377 0.606 Yes 1402 0.358 - 1420 0.186 - 1439 0.338 - 1446 0.405 - 1450 0.199 - 1473 0.359 - 1507 0.128 - 1545 0.693 Yes 1564 0.064 - 1580 0.549 Yes 1636 0.323 - 1704 0.712 Yes 1822 0.784 Yes 1867 0.364 - 2053 0.097 - 2091 0.666 Yes 2095 0.059 - 2206 0.330 - 2218 0.192 - 2247 0.768 Yes 2264 0.214 - 2287 0.146 - 2290 0.317 - 2327 0.157 - 2338 0.428 - 2344 0.187 - 2349 0.215 - 2368 0.399 - 2403 0.576 Yes 2493 0.212 - 2500 0.322 - 2528 0.307 - 2531 0.395 - 2606 0.056 -

Explain the output -- Processing locus 96: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAGGCCACGACATTCTAAATCCTGATCCAATCGCTTCACCAACTAATAAATTTTCTCAATTTTGGACAGACAAAACAAT GAAACCTATCGATAATAATAACCACACAAACCCCAAATAAACCCTCAGCTTCCTTATCCCTTCTTCTGGGGACCAAAAAA AACACAGAAGAGCTTCTTCTTCTTCTTCTTCTACATGGCAGGAGAATATTCCGGTAGCCGGAGTTCTAACACACAGCTTC TCGCGGAACTTGAAGCTCTAAGTGAAAACCTTTACCAGAAACCTCAGGTTTCAGTTGGAAACAGGAGAACAAACTCTCTC GCTCTCCCTCGGAGTTCTGTTCCGTCGCTTGTTACCTCTGCTGATGAAGTGAGTACAGCACGAGCTGAGGATTTGACAGT ATCGAAGCCGCGGGCTCGGCGTTTATCGCTGTCTCCGTGGCGGTCGAGACCGAAACTTGAGGTAGAGGAGGAAGAGAATG TTACTCAGAGCAACAGGATTGTCAAGAAACCAGAGGAGTCGTCATCGGGTTCGGGTGTGAAGGAAGAGAAGAAAGGGATT TGGAATTGGAAGCCGATTCGAGGGCTGGTTCGGATTGGTATGCAGAAGCTAAGCTGTTTGTTATCAGTGGAGGTTGTGGC GGCGCAGAATCTTCCAGCGTCGATGAATGGTTTACGCCTTGGAGTTTGCGTGAGGAAGAAGGAAACTAAAGACGGTGCTG TTCAGACGATGCCGTGTAGGGTTTCTCAGGGTTCTGCTGATTTTGAGGAGACCTTGTTTATCAAGTGCCATGTGTATTAC TCACCGGCGAACGGTAAAGGTAGTCCAGCGAAGTTTGAGGCTCGTCCGTTTTTGTTTTATCTTTTCGCCGTGGATGCCAA GGAACTGGAGTTTGGAAGGCATGTGGTGGATTTGAGTGAGTTGATTCAGGAGTCGGTAGAGAAGATGAATTACGAAGGAG CTAGGGTTAGGCAATGGGATATGAATTGGGGACTTTCAGGGAAGGCTAAAGGGGGTGAATTGGCTTTGAAATTAGGGTTT CAGATTATGGAGAAAGACGGTGGAGCTGGGATTTACAGTAAACAAGGCGAATTCGGGATGAAACCGAGTAGTAAACCTAA GAATTTCGCTAATTCCTTCGGTCGAAAGCAATCGAAGACGTCGTTTAGTGTTCCGAGCCCTAAAATGACGAGCCGGAGCG AGGCGTGGACGCCAGCGAGTGGTGTGGAGTCAGTTTCGGATTTTCACGGGATGGAGCATTTGAATCTGGATGAACCTGAG GAGAAACCCGAGGAGAAACCGGTTCAGAAAAACGACAAACCGGAACAGAGAGCTGAAGATGATCAAGAGGAACCAGATTT TGAAGTTGTGGATAAAGGAGTTGAGTTTGATGATGACTTGGAAACTGAGAAATCTGATGGAACCATAGGTGAAAGATCTG TTGAGATGAAGGAACAACATGTTAATGTTGATGATCCACGGCACATAATGAGACTCACCGAGCTTGATTCAATTGCAAAG CAGATCAAAGCGCTTGAATCGATGATGAAAGACGAGAGTGATGGAGGTGATGGTGAAACAGAGTCACAAAGGCTAGATGA AGAGGAACAAACTGTGACAAAGGAGTTTCTTCAGTTACTTGAGGATGAAGAAACCGAGAAGCTGAAATTTTATCAGCACA AAATGGACATCTCTGAACTGCGGTCTGGCGAATCCGTAGACGATGAATCGGAGAATTACCTCTCGGATCTCGGTAAAGGC ATTGGTTGCGTTGTTCAGACACGAGACGGTGGTTACTTAGTCTCTATGAACCCTTTCGACACGGTGGTCATGAGGAAAGA CACTCCTAAGCTTGTTATGCAAATCTCCAAACAGATTGTGGTACTACCAGAAGCCGGACCAGCCACTGGATTCGAGCTAT TTCATAGAATGGCGGGTTCGGGTGAGGAACTAGAGTCAAAGATATCTTCGCTTATGGCGATAGATGAGCTAATGGGGAAA ACAGGGGAGCAAGTGGCTTTTGAAGGAATAGCGTCAGCGATTATACAAGGGAGGAACAAAGAGAGAGCTAACACGAGCGC AGCGCGAACTGTCGCAGCTGTTAAAACAATGGCTAACGCGATGAGCAGCGGAAGAAGGGAGAGAATAATGACCGGTATAT GGAATGTGGAGGAGAATCCATTGACTTCGGCAGAGGAGGTTTTAGCCGTCTCTTTACAGAAACTAGAGGAAATGGTTGTT GAAGGACTTAAGATTCAAGCTGACATGGTGGATGATGAAGCTCCTTTCGAGGTTTCGGCTGCGAAAGGTCAGAAGAATCC ACTTGAATCGACCATTCCGCTGGAAGAATGGCAAAAGGAGCATCGCACACAGCAAAAGCTAACGGTTTTGGCGACAGTAC AGCTCCGTGACCCGACAAGGAGATATGAGGCTGTAGGAGGGACAGTTGTGGTAGCAGTACAAGCGGAAGAGGAGGAGGAG AAAGGGTTAAAGGTAGGAAGTTTGCACATTGGGGGAGTGAAGAAAGATGCGGCGGAGAAGCGGAGGCTTACCGCGGCGCA ATGGCTTGTGGAACACGGAATGGGAAAGAAAGGGAAGAAGAAGAGTAATATAAAGAAGAAGGAGAAGGAAGAAGAAGAAG AGGAGATGTTGTGGAGCTTGTCATCTAGAGTAATGGCGGATATGTGGCTTAAATCTATAAGGAACCCTGATGTGAAATTG CATTAGTTGATCTTGGAGGTATTCATACTATACAATAAGATGCATTTGTTTATTTATGTTTTGTATATTATTTTTTAAGA GTTTCAAATTTTACCTGCAACAAAC ..............................................................................N. ................................................................................ ..................................i............................................. ................................................................................ ...........................................N.................................... .............................................................................N.. ................................................................................ .......................................N........................................ ......................i...N..................................................... ........i............................................................N.......... .........................................................................N...... ....................N...........................................N............... .............i......i........................................................... ......i..................................................N...................... ................................................................i............... ..................................................i..................N.......... ..........................................................N..................... .............................N..N.......................N....................... .....i............N.....N.....N................i................................ .....................i..i...............N........N..........................N... ............................................i................................... ..i.......................................N..................................... .............................................N.......................N.......... ................N............................................................... ........i............................................i.........N.......i........ ................................................................................ ............................i...........N..........................i..........N. ...N...................................................................i........ ........................i......N..N............................................. ...........................i.................................................... ........................N....................................................... ..............................................N................................. i..................i............................................................ .....N..........................N........N...........................N.......... .......................................N...............N........................ ......................... Pos Score Pred ------------------------ 79 0.432 - 195 0.566 Yes 364 0.386 - 478 0.281 - 600 0.437 - 663 0.617 Yes 667 0.181 - 729 0.512 Yes 790 0.150 - 874 0.230 - 901 0.171 - 945 0.422 - 974 0.579 Yes 981 0.574 Yes 1047 0.621 Yes 1098 0.413 - 1185 0.727 Yes 1251 0.726 Yes 1270 0.058 - 1339 0.369 - 1390 0.058 - 1393 0.182 - 1417 0.066 - 1446 0.783 Yes 1459 0.092 - 1465 0.173 - 1471 0.112 - 1488 0.664 Yes 1542 0.611 Yes 1545 0.606 Yes 1561 0.344 - 1570 0.154 - 1597 0.072 - 1645 0.538 Yes 1683 0.697 Yes 1723 0.298 - 1806 0.449 - 1830 0.436 - 1857 0.453 - 1929 0.764 Yes 1974 0.538 Yes 1984 0.301 - 1992 0.792 Yes 2109 0.795 Yes 2121 0.374 - 2148 0.688 Yes 2159 0.495 - 2164 0.319 - 2232 0.853 Yes 2265 0.753 Yes 2272 0.276 - 2275 0.360 - 2348 0.620 Yes 2425 0.302 - 2527 0.432 - 2561 0.657 Yes 2580 0.546 Yes 2646 0.224 - 2673 0.328 - 2682 0.084 - 2710 0.051 - 2760 0.089 - 2776 0.048 -

Explain the output -- Processing locus 97: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGATCAAAGTTTTATTCATATGCTCAATAACATTTTTATGATTAAATTAAGCATACGTCGAGCATCATTAGTTAGGTTC CAAGTACAAAAGCCCATTTAGATTATTTTAGCCCGTTTAAGGCCCAATATGTTCTGCTTCACCTCTTGCTTAGGAGCCGT TGCCGACTTCTGGGAGCGACGCCGACGACGAAAGGGTAAACACGCCGCCGTTGATTCAGCCGGAGAATCAATGGAAAGTG AAGCAATTCAAGAGGATCTTCAGAATTTAGACGTCGAGTTAAAGGATGTTCAAGGTCAGATAAGTGCTTTGATTGAGCAT CAAGATAGGTTATACGAGAGAAAGTCTGAGTTAAAGACATTGTTAAAAGCATTAGCAGCATCAGGAAGTCCTGTTGCATC ATCTGGTGGCTCTAGTGCTATAGAGAATTGGTCTGAGACATTTGAGTGGGATTCTCGTGCTGACGATGTTAGGTTTAACG TTTTTGGTATATCCAAGTATAGGGCTAACCAGAAGGAAATTATTAACGCTATAATGACGGGAAGAGATGTCCTGGTGATC ATGGCAGCAGGTGGTGGGAAGAGTCTTTGCTATCAACTTCCAGCTATGCTTCGTGGTGGGACAACTCTTGTTGTCAGTCC TCTACTTTCACTGATTCAGGATCAGGTTATGGGTTTGGCTGCTCTAGGAATCTCAGCCTACATGCTGACTTCTACTTCTG GCAAGGAAAATGAGAAATTTGTGTACAAGGCACTTGAAAAGGGTGAAGATGACCTCAAGATACTTTATGTAACACCCGAG AAGGTATCAAAGAGCAAAAGATTCATGTCGAAGCTTGAAAAGTGTCACAATGCTGGCCGCCTCTCTCTTATATCAATTGA TGAGGCTCATTGTTGTAGTCAGTGGGGTCATGATTTTCGCCCTGATTACAAGAACTTAAGCATTCTGAAAACCCAGTTTC CCAAAGTTCCCATGGTGGCTTTAACTGCAACTGCTACACAGAAAGTTCAAAATGATCTGATAGAGATGCTGCATATTCCT AAATGCGTCAAATTTGTGAGCAGTGTTAACAGGCCAAATCTCTTTTACTCGGTCCGAGAAAAGTCGGCAGTTGGTAAATT AGTGGTTGATGAAATTGCAGAATTCATACGGGAATCATACTCTAACAATGAATCTGGGATAGTCTATTGTTTCTCCCGGA AGGAGTGTGAACAGATTGCAGGTGATTTACGAGAGCGAGGGATTTCTGCTGATTATTACCACGCAGACATGGATGCAAAT ATGCGCGAAAAAGTTCATATGCGATGGAGCAAGAACAAGTTGCAGGTCATTGTTGGAACAGTTGCGTTTGGTATGGGAAT CAACAAGCCTGATGTCAGGTTTGTCATCCACCACAGTTTGAGCAAATCAATGGAGACTTACTACCAGGAGAGTGGCCGTG CCGGTCGTGATGGCCTCCCATCCGAATGTATCCTCTTTTTTCGTTCGGCTGATGTTCCCAGGCAGAGTTCCATGGTCTTC TACGAGTATTCTGGTCTCCAAAATCTCTACGATATAGTAAGATACTGTCAGTCTAAAACAAAATGTCGTCGAAGCGCATT TTTCCGTCATTTTGGGGAACCATCACAAGATTGCAATGGAATGTGTGACAACTGTGCCTTGTCAAGTGAGGTCAAGGAAG TTGATGTATCGGACCTGTCTAAGCTCGTGGTTTCTATGGTGCAAGAAACGCAAGCCAAGGATCAAAGAGTAACGATGTTA CAGTTGGGTGACAAACTCAGAAATAAGCACAAGGATCTAATTGCTGAGTTAAAGAGAGACGAGGTAGAACATCTCGTGAT AAAACTGATTGTTGACTCGGTATTGAAAGAAGAATTTCAGCACACACCATATTCGACAAACGCTTATGTAACAATGGGAC CATTGGCCAACCAATTGTTGCAAGGAAGAAAGACAATCAAAATGGAAACCTCTAGCAGGCAAACCAAGAAATTGAAAAGA AGTATTACATTTTCCGGGTTGGAACTCAAGCTCGACGAGCTACGGAAAGAGATATCTGCAGCTGATGGAAGCATACTCCC TCACACAGTCCTCTCAACCCAGCAGATTGGCTCGATAAGTTCCCAAAAACCAGTTTCATTACAAGAGTTGGAGAGTATTA TAGGAAAATTGAAAACAGAAAAGTATGGAGACAGGATTCTAGAAGAAGTGATGAGACACGAAGCAGTCTCTGAACAACTT GTTGAAGATCCAACAAAGGAAGAGACATGTAAAAGCAGATTAAGAAAGAGAGCTAAGACACAGAAGGATGTTGTATTGGT AGAGAGCAGCGGAGAAGAAGAAGCTTGATGGATCAATTATCTTCTAATCCAATCATCGTAGCTGTCAAGGTTCCACATTT ACACTATTCTTATGTCAGATTTTAACCAATTCTAATTAAGAAATTCC N...................N.................N......................................... ................................................N............................... ......................................................................i......... .............................................N.................................. ................................................................................ .................................................................N.............. .....................................................i............N............. N............................................N.................................. ............................i................................i.................. .........N......................................N.................N............. ........................N........................i.............................N .............................N.................................................. ...........i.......................................N.............N.............. ..N............................................................................. ........N......................................i................................ ....................................................................i...N....... i.................N....N................................................i....... ...........N.....................................i.............................. .........i...............N.........................N...................N........ ..............................................................i................. ...................................N....i....................................... ...N...............................N......................................i..... ................................................................................ .................................................................N.......N...... .........................................N...................................... ................................................................N............... ................................................................................ ........................N.........................N............................. ..........................N........................................N............ ...........................N.................................................... ...........N................................... Pos Score Pred ------------------------ 1 0.188 - 21 0.374 - 39 0.172 - 129 0.452 - 231 0.583 Yes 286 0.431 - 466 0.239 - 534 0.760 Yes 547 0.119 - 561 0.464 - 606 0.413 - 669 0.761 Yes 702 0.576 Yes 730 0.221 - 769 0.153 - 787 0.122 - 825 0.428 - 850 0.687 Yes 880 0.110 - 910 0.083 - 972 0.752 Yes 1012 0.237 - 1026 0.410 - 1043 0.288 - 1129 0.204 - 1168 0.802 Yes 1269 0.715 Yes 1273 0.108 - 1281 0.765 Yes 1299 0.328 - 1304 0.499 - 1353 0.556 Yes 1372 0.415 - 1410 0.724 Yes 1450 0.528 Yes 1466 0.216 - 1492 0.214 - 1512 0.273 - 1583 0.507 Yes 1636 0.384 - 1641 0.756 Yes 1684 0.359 - 1716 0.332 - 1755 0.510 Yes 1906 0.166 - 1914 0.404 - 1962 0.403 - 2065 0.306 - 2185 0.435 - 2211 0.487 - 2267 0.426 - 2308 0.411 - 2348 0.196 - 2412 0.041 -

Explain the output -- Processing locus 98: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCCTTCTCTCCTTCATTGAACGTTTTTGTATATTGTTCTTTGATTATTCATACTCAAATATAAAGTTTGATGTTTGAAAT TATGCTCTATCTCCTTTGTAAATGTGTTGCAGAAGACATCTTACAGAGTCAATGGAAACAAACCAGTGGCGGTCGAGGAA AAAGATAGAATCAGCTGCAGAAACACTCCAAGTTTCTTCTAGAAGAGGCAGAGGACAGGCACGTATGGTTCCTCCTGTAA GTGGAGTTAGATCCGAGAGAGCTCGAAAAAGTCTAAGTGAGAAGCTAGAGACTGTAGCTTTAAACTCTCCTAAGAAAGAT GCTCGTGTGAGTCTTTATGGCGAGAAAAGTGTAGTAGATGAGATTTTCCTAGAAGATGAAGAAATGGGTCATGAAACTGG TTTGAAGAATGGAGAGTCGTCGCCTTTTTGTGGTGTATCGGACAAGCTTCTGCAGCGAATCGAGCTTCTTGGGAGAGACC ATGAGGCAACGAGACTTGATAACAACAAGTTCAGGTCAATCGAATCTATGAAAAAGAGACAAGAAGAATCGGCATGTGAT GATCTAGTTGATATGAAGACAAAGATTCAAACGTTAGCTGCGGAGAACACTCAGCTTAAGAAGTCTCTTGTGGCGAAAGA AGAGCTTGCGGTAAGTCTTCAAGAGCGTAAATTTCAGGTGGAATCAGAATTCGAAGCGTTAATGACTAGATTAGATTCAA CGGAGAAAGAAAACGCATTCTTGAGATATGAGTATACTGTTCTTGAGAAAGATCTTCAGGTAAAAACAGAGGAAACAGAG CATACTCGCAGGTCTATGGAGCTAACGCATAAGCAGCAACTTAGGAATGTGAACAAGATTGTTGAGCTTGAAGCTGAATG CCAAAGGTTAAGACTTCTCTTTCGAAAGAAGTTTCCAGAGAAGTCTATATCTATGAGGAATGAAGGTGAAGAAAAGAAGA TGGAGATGAGAAGACGAAACGCTAATAAGAGCGATATGATGATGAGAGATGAAGTTCAGAGCAGGAAGCTGAAGTATGAT CTTTTGATGGAGCAGATTGGGAATGTTAGGGCAGAGAACAAGAATCTAATGGACATAATTATGAAGAAAAACATTGAGAT CAAAGATCTTAGCCGCGGGCAGAAACCGTTAGAGGCTTCGAGTTTCGATATTCAAAGCGAAAGTAGTGTGATGAGCCCCT GTGGTTCCAAGGAGATGAAGTTACTGATGGATGATTTTAATGAGATGGAGAAGTTAGCCATTGTGTGTACTGAGAAAGAT CCAAGAGTGGATGATGAGAAGGAAGGATCTTTTGATTGGATTCAAGTTGTTTTGAGCGCTATAACGAAGCAAGAGAGGAT ATCGAAACGCGGTGTTAAAGAACTCTTACAAGACATTAAGATTGCTTTAGGATGTATGGATGAAAATGATAATGTAGAAA GAAAGAAAGGTGAAGAAGATCCTCTCTGCATCACATGGAAATCTAATAACGAATCGGGTCCAATGACTAAGGATGAGATC AAGAGACATTTGGGTTTGACGAAATCAGATAAGGTGGAGAAGATTGAATCTGATGAAAAGCAAGAACTGAGAAAGAAGCT TGAGGAATCAGTGGAGAAGATCAGAAACTTAGAAGCAGAGATGAAAACATTGAGAGAGAATAAGGAAAAGGTAGAGGCAG AGATGGAAACGGAGAAATCGATGAAGGAAGATCTTGATACAAAGCTGAACATAACAAGAGCTAACCTCAACGAGACACAG AAGAAGTTGTCTTCTCTTGAAGTAGAATTTGATTACAGAAAGAGCTGTTGTGAAGAACTCGAAGGGACTTGCATCGAGCT TCAGCTTCAGCTAGAAAGTGTTGAAACAAAGAAACCGACGCAGAGAAACAAAAACGGGTGGGATATAGCAACAGCTTCTG TGAAGTTATCAGAGTGTCAAGAAACGATTACCAGTTTACGGAAGCAACTAAGAGCGTTATCTACAACAGAAACAAGCAGC ACCATTAAATTTCTACACAAAAGATCTTCTCTCCGGGAAAATATAGCAGAGGATGATACTAACAGAGTTGCTCAAGATGA TGATGGTAACCGTTACAACGCTTTGATCGTGTACGAACCAGTGAAGGCGAGAGGTGAGAAGATGGAGATGGTACCGAGAA AGAAGCAAGGATTAGGGTTCTTGAAAAAACTGTTGTTCAGGAGGAAAAGAGTGAGCAGCAAGAAATGTCTTGCCTTGACT ATGTGATGAAGAAAACTAAACTAAAAAGGCTTGTGTGTGTGTAATGAGTGAACAGTGAAAACTGCTTTTGGGTATGTTTG ATGTGTCTACTCTATGAGATGTGAT .....................................................................N.......... ................................................................i............... ..............................................................................N. ................N....................N.................N.......i......N......... ........N....................................................................... N..............................................N.........................i....N. ............N................................................................... .............................................................i.................. ...........................N.................................................... ...............N..............................N..............................i.. ....................................................i......N...................i .....N.............................i..N..N......N..........................N.... ......N...............N.........................N...........N................... ......................................................................i......... ..............N...........N...N........N....N................................... ..........N..N.................................................................. ...................................................N...i...N.....N.....N........ ..................................N...........................i.........N....... ....................................................N........................... ........................................N....................................... ..i.................N........................................................... ................................................................................ ................................................................................ ................................................................................ ....................................................N.......................N..N ..N..........................................................N.....N............ ................................................................N............... N....N.....................................N.............................N...... N............N....N...... Pos Score Pred ------------------------ 70 0.294 - 82 0.412 - 102 0.540 Yes 132 0.632 Yes 225 0.744 Yes 319 0.231 - 337 0.166 - 358 0.199 - 376 0.340 - 384 0.792 Yes 391 0.297 - 409 0.373 - 481 0.212 - 528 0.350 - 554 0.670 Yes 559 0.122 - 573 0.434 - 702 0.606 Yes 748 0.236 - 816 0.260 - 847 0.392 - 878 0.605 Yes 933 0.646 Yes 940 0.313 - 960 0.751 Yes 966 0.396 - 996 0.521 Yes 999 0.243 - 1002 0.452 - 1009 0.174 - 1036 0.245 - 1047 0.337 - 1063 0.151 - 1089 0.390 - 1101 0.316 - 1191 0.627 Yes 1215 0.471 - 1227 0.477 - 1231 0.079 - 1240 0.247 - 1245 0.410 - 1291 0.101 - 1294 0.416 - 1412 0.222 - 1416 0.709 Yes 1420 0.189 - 1426 0.280 - 1432 0.217 - 1475 0.440 - 1503 0.773 Yes 1513 0.180 - 1573 0.129 - 1641 0.351 - 1683 0.588 Yes 1701 0.292 - 2053 0.322 - 2077 0.251 - 2080 0.191 - 2083 0.155 - 2142 0.475 - 2148 0.073 - 2225 0.342 - 2241 0.079 - 2246 0.099 - 2284 0.087 - 2314 0.047 - 2321 0.050 - 2334 0.025 - 2339 0.037 -

Explain the output -- Processing locus 99: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAAGGAATGGAAAAATCGAGAAAAATGGTATCGTGTCGCATCGAGGACTTATTACGTATGGCGAGAGAGCTCAAACCA AAAGATCACCGCCTCGACTGATTCATCTTTTCTCCTGGTATGTATTTTGGAAAATTCGTCTCTTGCTTTTTATTTGATCC AGATCAGAAAACGTTGTGCAGATTGATTCTGGGTTTATCAGTTCCTTACCACCATAGTACTCTCCATGTTCTTCTCAATT GTAAATTCCTTTCCTTTTTGTGTGTCAATTTACACATATCTGATATCTTCTTCGTGTTGCTCCCAAATTTTGCAGATTCT TGGCAGGCTTCTCTTGGTCTAAGTGAATTTGGAGTTATGCTTCTGTCAGCACTTTTAACTTCAGTGGGAATCAATCTTGG CCTCTGTTTTCTCTTCTTCACTTTGTATTCAATATTGAGAAAGCAGCCTAGTAATGTCACTGTATACGGTCCACGTCTTG TTAAAAAAGATGGCAAGTCTCAGCAGTCAAATGAGTTCAACCTCGAGAGGCTTTTGCCTACTGCTGGTTGGGTCAAAAGA GCATTGGAACCTACCAATGATGAAATCCTCTCCAATCTTGGCTTAGATGCTTTGGTCTTCATTCGTGTCTTTGTCTTCAG CATAAGAGTGTTTAGCTTTGCATCTGTGGTTGGGATCTTCATACTTCTTCCAGTGAATTATATGGGAACAGAGTTTGAAG AGTTTTTCGACCTCCCAAAGAAGTCTATGGATAACTTCAGTATCTCTAATGTTAACGATGGATCAAATAAGCTGTGGATC CACTTTTGCGCAATATACATCTTCACGGCGGTTGTTTGTTCTCTACTTTACTATGAGCACAAGTACATTTTAACAAAGCG GATTGCTCATCTTTATTCATCCAAGCCTCAGCCACAAGAATTTACAGTTCTGGTCAGTGGCGTCCCTCTTGTATCCGGGA ACAGTATTAGTGAGACAGTAGAAAACTTTTTCAGGGAATATCATTCTTCCTCGTATCTTTCTCATATAGTTGTTCATCGG ACAGACAAGTTGAAAGTTCTCATGAATGATGCTGAGAAGCTGTACAAGAAGCTTACACGAGTAAAATCCGGAAGCATATC TCGCCAAAAGTCTAGGTGGGGTGGGTTTCTAGGGATGTTTGGGAATAATGTTGATGTGGTTGACCATTACCAAAAGAAGC TTGATAAGTTAGAAGATGACATGAGATTGAAACAGTCTTTATTAGCAGGAGAGGAAGTTCCAGCTGCTTTTGTTTCCTTT AGGACAAGACATGGTGCTGCAATAGCAACAAACATTCAGCAAGGAATAGATCCAACACAATGGCTAACTGAGGCAGCCCC GGAGCCCGAAGATGTTCATTGGCCATTTTTCACTGCATCGTTTGTGAGAAGATGGATCTCCAATGTAGTGGTGCTTGTGG CTTTCGTAGCTCTTTTGATCCTATACATTGTCCCCGTTGTATTGGTTCAAGGTCTTGCTAATCTTCACCAGTTGGAGACT TGGTTCCCTTTTCTAAAAGGAATATTAAATATGAAAATCGTGAGTCAAGTGATTACGGGATATCTTCCGAGTCTCATTTT CCAGCTTTTCCTTCTTATAGTACCACCCATTATGCTTCTACTTTCCTCAATGCAAGGATTCATTTCTCATAGCCAGATTG AGAAATCTGCATGTATCAAGCTTCTAATCTTTACTGTATGGAACAGTTTCTTTGCAAATGTACTGTCCGGATCTGCCCTT TATCGTGTTAATGTGTTCCTTGAACCTAAGACTATTCCTCGTGTGCTTGCTGCAGCTGTGCCAGCACAGGCATCGTTCTT TGTATCTTATGTTGTGACCTCTGGATGGACTGGTTTATCATCAGAGATCCTCCGTTTGGTTCCTCTTCTTTGGAGTTTCA TAACGAAACTTTTCGGCAAGGAAGATGACAAAGAATTTGAGGTTCCTTCAACTCCTTTCTGCCAAGAAATCCCAAGGATT CTATTTTTTGGACTCCTCGGTATAACTTACTTCTTCCTTTCGCCATTGATACTGCCTTTCTTGTTGGTCTACTATTGTCT TGGATATATCATCTACCGCAACCAGCTCCTAAACGTATATGCGGCCAAGTATGAAACTGGTGGAAAGTTTTGGCCAATAG TTCACAGCTATACTATCTTCTCTTTGGTACTAATGCACATTATTGCAGTCGGATTATTCGGGCTTAAAGAGCTTCCAGTG GCATCTTCTTTAACAATTCCCCTTCCGGTTCTCACGGTCCTTTTCAGCATTTACTGCCAAAGACGGTTTTTACCAAATTT CAAATCTTATCCTACCCAGTGTCTGGTAAACAAAGATAAAGCAGACGAGAGAGAGCAAAACATGTCTGAATTCTATTCGG AACTCGTTGTTGCTTACCGAGATCCTGCACTTTCGGCATCACAGGACTCGAGAGATATCTCACCTTGAGATCACCTTTTT ACTTGAGCTATTGAAGTTTGCTTATGTTGAAGCTGTTGGTTTACTATACCTGGTGCAATCCATGAAAGAACACACTAAAC CGGTTGT ........N.................N................................N.................... .......................................N........................................ .................................................................N.............. ................................................................................ ....................................N........................................... .....................................................i.......................... .........i....................N................................................. ................N..N..........................N................................. .............................................................N.................. ..........................N.....................N........N...................... ....................................................N........................... ................................................................................ ................................................................................ .....................i...N..N................................................... ..................................i............N.....N.......................... ...............N....i........................................................... ..........N................................................i.................... ...........N.......................................N..........N................. ................................................................................ ..............................N................................................. ...............................N.................N.............................. ..........N..........................N...................N...................... ..........N..................................................................... ........N...............N....................................................... ........................i....................................................... ................................................................................ ......................................N...........N............................. ................................N............................................... ................................................................................ .............................................................i.................. ................................................................................ .......................N.....................................N.................. ....... Pos Score Pred ------------------------ 9 0.449 - 27 0.443 - 60 0.426 - 120 0.477 - 226 0.156 - 357 0.317 - 454 0.501 Yes 490 0.635 Yes 511 0.404 - 577 0.360 - 580 0.128 - 607 0.144 - 702 0.423 - 747 0.395 - 769 0.176 - 778 0.490 - 853 0.176 - 1062 0.684 Yes 1066 0.307 - 1069 0.398 - 1155 0.574 Yes 1168 0.147 - 1174 0.102 - 1216 0.263 - 1221 0.800 Yes 1291 0.336 - 1340 0.660 Yes 1372 0.106 - 1412 0.461 - 1423 0.178 - 1551 0.160 - 1632 0.184 - 1650 0.342 - 1691 0.452 - 1718 0.220 - 1738 0.231 - 1771 0.121 - 1849 0.222 - 1865 0.296 - 1945 0.680 Yes 2119 0.233 - 2131 0.211 - 2193 0.161 - 2382 0.537 Yes 2504 0.064 - 2542 0.054 -

Explain the output -- Processing locus 100: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAAACGATAAGAAATGGGTCAGGGCAAGTTGATGTTGTAAAAGTCAGAACCACAACGTGAGAGAGAGAGAGAGAGAAAG AAAGCAAAAAAAGTTCGTTAGCTTGTGAAACTTGTCACAAGTTTCATCACTATATTTATAACTAACCTTATGTTGAAGAG AAAGCACCTCAACACAAGACCAATGTCAATCTCTTGTACCAGAAACTTCTTCAAACGATTCTGCGTCGAAGAATACAATA TGGATACATTCAAACATAGCAGTTTCCTCTCTGCTGATCTCTTACCATCTCTTGGAGCTAGAATAAACCAATCAACTAAG CTCCGTAAACACATCATCTCTCCTTTCGATCCACGTTTCAGGGGATGGGAGATGTGGCTGGTCATTCTTGTGATTTACTC AGCTTGGATTTGTCCATTTGAGTTTGCCTTCATCACCTACAAGAAAGACGCACTTTTCATCATTGACAACATTGTCAATG GATTCTTTGCCATTGACATCATTCTCACCTTCTTCGTTGCTTATCTAGACAGCCACTCATATCTTCTAGTCGACAAACCT AAGAAAATAGCTATAAGGTACCTTTCTACTTGGTTCGCCTTTGATGTCTGCTCAACAGCTCCATTCCAGTCACTGAGTCT TCTGTTCAAATACAATGGAAGTGAAATAGGATTCAGAGTTCTTAGTATGCTCAGGCTCTGGCGTCTTAGACGAGTTAGCT CGCTGTTTGCAAGGCTTGAGAAAGATATCCGCTTCAACTATTTCTGGACACGATGCACAAAACTCATTTCGGTGACACTG TTTGCAGTACATTGTGCTGGATGCTTCGCCTACCTCATTGCAGATCAATATCATGATCCAACAAAAACATGGATTGGAGC TGTCTACCCAAATTTCAAGGAGACAAGCGTATGGAGTAGATATGTTACTGCTCTTTACTGGTCTATTACGACATTAACAA CAACAGGTTATGGAGACTTACATGCTGAGAACCCAAGAGAAATGTTGTTCTTTGTGTTTTTCATGCTCTTCAACCTCGGT TTCACATCTTACCTCATAGGAAATATGACCAACCTTGTGGTTCACTGGACTAGCCGCACCAGAAACTTTAGAGATACTGT AAGAGCTGCTTCAGAGTTTGCATCAAGAAACCAACTCCCACCAAACATACAAGACCAAATGTTATCACACATTTGCTTAA AGTTTAAAACAGAGGGTCTGAAACAACAAGAAGCTTTAAATGGTTTGCCGAAAGCAATCCGGTCAAGCATTGCAAACTAT CTCTTCTTCCCAATCGTTCAGAACGTCTACCTATTTCACGGAGTTTCTCGCAACTTCCTATTTCAGTTGGTTTCAGATAT AGACGCCGAATATTTCCCACCAAGAGAAGATGTAATACTACAGAACGAAGCTCCTACTGACCTCTACATCCTAGTCTCAG GGGCAGTGGATTTTACTGTCTACGTTGGCGAAGAAGATCAGGTTCAAGGCAAGGCGGTTGTTGGCGATGCATTTGGAGAG ATTGGTGTGTTATGCTATACACCACAACCTTTCACAGTTAGGACAACAGAACTATCTCAGATACTACGGATAAGCAAAAA ATCTCTAATGAGTGCTATGCGAGCTCATGTCGAAGATGGACGAGTCATAATGAACAACCTCTTCATGAAACTTAGAGGGC AACAATCAATAGCAATTGATGATCCAAATTCAGAACCAGAGTCACTTCTCAAGGAGTGGCTTGTTGGTGGTTCAAAGACA GGAGAAGGAAACGCTAGTGATCAAGGACATGGGCACAAATATTTACAACTGCATGATTCAGAAAATATTGATATGGGGTC AACTGAATGGAGAGATTCAAGAAGATCTGGTTATGGCGAAACGAAAAGAGTTCGAGAACATACGATAGAGATAGAAGAAG GAGAAAAACCAAATAAAGAGTTTGATGGAAAAGGTTGCTCTGATGCAGATCTTACCTCATTTGAATTTCATTCACAGGAA GCATATCCATATTGCAGATCAAACATCCAAATCAAACAACATGAAGCTGCAAAACCAAAAGATAAAAGAGTTACCATCCA CTTGAAGTCCCGGGACAAAGATTTGTCGAAGCTGATAATCCTTCCTGCTTCCATAGAAGAGTTGCTAAGACTTGCAGGTG AAAAATTTGGATACAGCTTCACAAAGGTCACGAATGCGGAAAACGCAGAAATAGATGATGAAGATGTGATTAGAGATGGT GATCATTTGTATATTCTCATCAATGAAAACTCTTAACAAGACTTTAGTAACCACTGTTTTCTTTCCATAGAGTGTATATG TATATCTGCAGGCAATGTAATGTTTTTTACTAGAAAAACATTTGATCATCAAAGA ..............i.................N............................................... .....................................................................N.......... ......................i........................................................i ................................................................................ ............................................N......i............................ .............................................................................N.. ................................................................................ ...........................................i.................................... ..............i...............................N................................. ....................................................N........................... ....................N...............................N...............N........... ..............................N..........N...................................... .........N...........N...................i....................N................. ........................i....................................................... ..........................................................N..................... .......................................N........................................ ................................................................................ .............................N.................................................. ..................................................................N............. ...........i.................................................................... .......i........i.........N........i.............i..............N............... ..................N............................................................. ............................N.......................N...................i....... ......N.........................N............................................... ........................N.................N..................................... ........................................N....................................... ................................................................................ .................................N....................N..N.....N...........N.... ......................N......................................................N.. ..............N....N................................... Pos Score Pred ------------------------ 15 0.720 Yes 33 0.220 - 150 0.171 - 183 0.591 Yes 240 0.752 Yes 365 0.389 - 372 0.533 Yes 478 0.449 - 604 0.533 Yes 655 0.676 Yes 687 0.155 - 773 0.170 - 821 0.259 - 853 0.225 - 869 0.349 - 911 0.425 - 922 0.140 - 970 0.417 - 982 0.197 - 1002 0.757 Yes 1023 0.249 - 1065 0.583 Yes 1179 0.457 - 1240 0.212 - 1390 0.238 - 1507 0.147 - 1532 0.557 Yes 1608 0.603 Yes 1617 0.590 Yes 1627 0.160 - 1636 0.703 Yes 1650 0.542 Yes 1665 0.335 - 1699 0.448 - 1789 0.324 - 1813 0.309 - 1833 0.763 Yes 1847 0.344 - 1873 0.336 - 1945 0.402 - 1963 0.245 - 2041 0.198 - 2194 0.256 - 2215 0.158 - 2218 0.442 - 2224 0.281 - 2236 0.306 - 2263 0.181 - 2318 0.039 - 2335 0.045 - 2340 0.047 -

Explain the output -- Processing locus 101: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACCGAACCAAACCCTCTTTAACCGGAACTCCCACTCTTGCAATAAAATAACAAAACCAATGAACCAAAATCGCCGCCGA AAGGGCAAAAATCGTTAATAAACAAACCACACGGAAGAAGATGAGTCCCGTCACAATCAAATCAGGCCAATCTCACGTTA TCCCAAACCAAAACCAAGTAAACCGGAAATTCCACTCTTACTTTTAACAACAACAAACCAAAAAACACGATCGCCGCCAC AAGGGCAAAACCGTCAATTCACAAGTAGATCCTGTCACGTGATCCCAATGATAAAACCAATCCTCTCTCTCTCTCTCTTT TCCCGTAGTCCTTTTTCAGTCATGGCGATAACCGCATACGACGCTCTCACGGAGAGACAAAAAGCCCCCAAAGTCCTCCT TATTAACGATTTTGCCATCTCTTCTTCTTCATCATCACCACCTTTCTTCAACCTCGCCGCAACTTTCCGCGACAACATCA GATCGTTCCTCCGTGAATATGCCGAGATTGAAGATTACACCATCGACGGAGCCACCGTCTCTACTATTTTCCTTGGAAGC CAAGCAAATGGCGTCGTTTTCCCTCTTTACATCATCGAACAACAAATCTCTGATTCTTCTCCAAACCCTCTCTGCGATTT CTGTCGATGCTTTGGTTGGGGTCATCACTATGTAACGAAGAGGAAGTATCATATGATAATCCCAAACCGTGATGAATGGA ATGAGCCATTGAAGAGAGAAAGCTTGACGCTTTCTTCACATTTGATGCATGGTTTGATTCATTGCAATGGTTTTGGTCAT TTGCTTTGTATCAACACTGATCTTGATGATCCTAATCACCTCTCTGGTGACCAAATCATGGACTTTTGGGACCGTCTTTG CTCTACTCTCCACACTAGGAAGATATCTTTGGATGATACATCGAAGAAGGGAGCGATGGATCTGAGGCTGCTCCATGGTG TTGCATATGGTCGACCATGGTTTGGGAAATGGGATTATATGTTTTCTCATGGAAGCTTTGGGGTCAAAAAAGATCTGTAT TGGCGAGCTATTCTCACTCTTAGCTCAATAGAAGTTGACAAGATATTGGAAGAATTATCTGGAACAAGTAAAGGGAGAGT GATGAAGAAGATCATCGATTTCTACAGAGGATCCACTGAAAGCCCTTTGGCCACTCTCTCTGATTTGCTTCGGTTCATGC TTGGTTTCATATCAAAAGCTCCCATTGAGAGAAAGACTGCAATGGCACTAGTAGCAATGTCTTTAGATCATGTTTCATAT CCTACTCTTAGAGCTGATGAGAACAGTGAGGTCTGCACATCCCCTGATCAGGAATCAGATGATAATGGATATGAATCTGG CAGAGACACTGTCCTTGATGATCATAATACTACAACATCGGGCATAAAACCTCCTCAGTATTACTCATTTGATGATTTGA GCAGGAGAGAACATTCTAGATGGCCTGGAAGGAGGTTGAATGATGCAGCCCAAGCGGTTCTCAAAGTGTTTAAAGAGCGA AATAGCACCATTAGTCGCCAAGACCTTCGGGAGGCTGTTAGAAGTAGCATTGGTGATACGGGATTGATTGATTTCTTGCT CAAACACATTGACAAAGTTCTAATAGGTGACCAGATAGTGCAGAGGTCCACAAACCCGAAAAGCAGGATGTTACAGTTCT CCCTCCGGACAATCAATTCCCGTGTTCAAGAGCAAAAACGGAAAAAGAAAAGGAAAGTGAAGCCGCAGGAAACCAGTGAA TGCACATCTACCACTCCTGGACTGAGTCCGTACGATGATATTCTCTATCTATATCAGAATCTCTTGTTAACTTATCCTGA TTCAGACTTATACAGTGAAGCGTCTCAGGTTATCTTGAAGTGCAAGAGCTTTGTAAAAGAATGGTCTTACCAGGAACAAA ATCATTTAACAGTTAGCTGCCAGGTTCTTCCCAATCATGAAGAACTACTCAGAGACTTCACTCGGTTATTACCCCCTGGT GACCTCGTGGCTGTTCCGGAAAACGCAACCATCAGAGAACTGAAATCTGCAGCAGAGAAAGTTCTGAGGGACACTTACTG CATAACAGAGACATTTGAAGTGTTGGAGATAAGAAACAGGTATCTCGAGAAGCTAGACGACAACGTGAGTCTAAAATCAC AAGGGAATACAGAGTTTATGGTGAAAGGGTTTGGTCTGGACATAGGCACAGAGTTAAGGTATGAAGGCGGATTTGATGAC TGGACCGTTGACTGCAAATGCGGAGCTAGAGATGATGATGGTGAGAGAATGGTGGCTTGTGATGCTTGCAAGGTTTGGCA CCACACACTTTGCAATTCCATCGAAGACGATGAAGCTGTGCCCTCCGTGTTTCTCTGTAACATGTGTTATGGAGATAGCT TGAGATCCAAGAAACGCAACCTCAGCATTCGCTAGCCCTAGCTTGCGCAAAAAGTCAGCTTGTGTTGCAGGAGATTATAT ATCACCCATTATTATGGTCAAAACTTACCAACCATAGCCATAGATCTAATTTTA ...........................................................i.................... ........................................i....................................... ................................................................................ ...............................................i................................ .....................i.......................................................... ................................................................................ ..................i............................................................. .......i........................................................................ ......i......................N......................N..................N...N.... N...........................................N...N.................i............. .........................N...............................i...................... ................................N......................i..................N..... ......N.........N...........N.........N.........N............................... ................................................................................ .........................................i..............i............i.......... ................N.........................................N.....N.....N......... .................N.....................................................N........ ...................i...................i..N..................................... ................................................................................ ...................................................................N............ ...............................................................................N ..................................N............................................. ............................................................N................... ....................................N........................................... ................................................................................ ................................................................................ .................i..........................................i..............i.... .................N.............N..N..N..........i............N.................. .............................N...............................i......N........... ................................................................................ .............N........................................ Pos Score Pred ------------------------ 60 0.647 Yes 121 0.586 Yes 288 0.591 Yes 342 0.817 Yes 499 0.751 Yes 568 0.660 Yes 647 0.557 Yes 670 0.160 - 693 0.262 - 712 0.175 - 716 0.388 - 721 0.302 - 765 0.472 - 769 0.286 - 787 0.563 Yes 826 0.199 - 858 0.729 Yes 913 0.317 - 936 0.546 Yes 955 0.417 - 967 0.334 - 977 0.236 - 989 0.232 - 999 0.313 - 1009 0.215 - 1122 0.315 - 1197 0.271 - 1242 0.753 Yes 1257 0.677 Yes 1270 0.520 Yes 1297 0.128 - 1339 0.118 - 1345 0.408 - 1351 0.305 - 1378 0.099 - 1432 0.157 - 1460 0.662 Yes 1480 0.537 Yes 1483 0.474 - 1668 0.355 - 1760 0.328 - 1795 0.148 - 1901 0.373 - 1957 0.137 - 2178 0.633 Yes 2221 0.584 Yes 2236 0.516 Yes 2258 0.165 - 2272 0.129 - 2275 0.215 - 2278 0.255 - 2289 0.790 Yes 2302 0.182 - 2350 0.474 - 2382 0.596 Yes 2389 0.481 - 2494 0.053 -

Explain the output -- Processing locus 102: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTACTATCATTACAAGAAGTTGTTTAGTAAATTTTCAGAGATTTCATTTTAGCATGTTTCATCTACTAATCATTGAAAGT GTCTTTATTTAGGAGGACTAAACTGTTTTCTTGTATTGTGTAATGTTTTACAGATGTTTTGAAGTGGTGATTGGAGTGGA AATGGAATTTGGTAAAGGAATTGATATGGAACAAAGCCCCAATAGTGTTCTTCCTCCTCCTCGTCCATTCACTGAATCAG AAAAGAGGACTAGTACAAGACTAAAGCCGCCTCGTAGAGATGAGATATTGAGAGTCAAAGAAGGTTTCACCGAGATAAGT TTCAGGCGTTACCGTAGCACATCATGTAAGAACTTTCCTTCTAAACCACTTGCAATGGGAGATACAACGGAGCTAAGGCG CGGTTCTGTTTATCAAAGCTCTAATGAGTTGTTTAAAGAACTTAGAGAACCACAAGGACGTAAAGATTCAAAGGCGAAAC TTGAGTTGTCTCGTAGTAGTGATGCTTCTTTCTCCTTTAGAGTTGTTGATTCTTCGAGGAAGGGAAGTACTGAGAAAAGA CCGCATAAGACGGTTTTAGATGGTCAGAAGTCATTAGTTGAACCTTGCACCTCTAGCAACTTCATTGACATTTGTTTGAA GTCAGGTATTAAAGATAGAGCTGTAGTGTTGGATTCAGATGATAATGACCATAAGATTAGATTGCCTAAGCCATATTCTG CAAAGGTAGACTCAGTAGAAACCAGTTACAATAAGGACAGCTCAAGAGTTAGGAAAATGTTTGATCCATTTGTTAAATCA AAATCTTTGAGAAGTCCTCTTGGTTATATAGGAGAATCAGGTGATCAGTTCAAATTGGGGAGGAACAGTGAGCGTTGCAG ATCTATGTTGAGTGACTACTCAAACATTCATAAAAGATCGAATCTTTGTCCTCCTCCAGTTGTCAATAAGGATTATACCT CGGTGCTGAAATCTTCCCCGGTTCATCTACATTGCCGTCTCAAGATGGAAAGCAAGAATGGATTACCGGTTTTTCAGTTT GTTTCAGATTCTCCTGAGGAAGTTTACGCTGCAAAGACGTGGAAATCTGACAATGGTTCTACTTGGGTATATACATTTTC ATCTGCTGGAAGCAGAAAGAGGAGCAGTGCTAGCGTGCGGGGTTTGACCGATGTTAGTAAAGAATCTTTGCTGGTGGCTC AGATGCAAGTTACCTGTAAGATGTGTTCAGAAGTAAGAAAAAAGGGACAAGATCCAGAAACTTTGATGGTGAATGAGTTT GTTTTGTATGATATTGCACAAGCAAGAAGAAGTGTCTCCACAAAGGAAGATCAATCACTACCACTTGATACGGTTAATAA TGCCTCCAAGAACTCTGTTAAATCAGACTCAGAGATAAGAAATAACTCAATGTCCGGGGATGCTTCGGATACAATGAAGC AGAGATCTCAGCCTAAAAGAACATCTCAAAGCTATGATCTTGAGGCTTCAAATGGAACAAACCCGTGGTCAGCTGCAAAT TTGCATCCTGACCTTGAGATCGCAGCTATAATTATTCAAGACACTATTGAGAAGAGAGAAAGCTTAAAATATAGAAGAGG TGACAAAAGACTGATGGAGAAAACTAATATTCTTGGTCTTTCTCCAATAGAAGAGGAGAAAAAGGAGTTGTTTGGCAGCA GAAGTTTAGAAAAACTGAAGGTTGTAATCCCGAGGGGAAACCACGGGTTACCAACTACCGAAAACTCTTGTCCTTCGCCT TTGATTCAGCGATGGAGGTCAGGAGGCGGTTGTGACTGTGGTGGTTGGGACATGGCTTGTCCACTTATGGTTCTTGGTAA TCCTCGTATCTCATGTTCTCATGATCAGCCTCTTGTAGAAAATCAGCATCCTTTGCAACTATTTGTCCAGGGTGCTAAAG AACATATACCAGCATTGTACATGTCATTTGTTGAGGAAGGACAGTACGATGTTCATTTCCACGCGCAGTTATCAACATTG CAAGCATTCTCCATCTGTGTTGCCATATTACATAACACGGAAGTCTCAGATAGTTACAGAAATGGCGAGAATGTACAACA GTTTTCGCATTGCAACTCGTTGAAAATGCTGATTGATGATGATGTTCAGTTCTTAGTTGAAGCAGTAACAGAGGAAGAAG AAGTCCCTAAGCCTCTGAAAGAAGCTGTGACCGCTCTTCAATCCTACATGCCGAATCCTCCTTTCTCGCCAATCTCCCGA GTGTAGTCTGTGATGCTATCACTATTGCAGGTGCATTTGAGTTGGCGATAAGAAAAGAAGAGGAGAGAAGGTTGGTCTCC AACTTCCCCGATAAAGCGCAACCAT .....................................................N.......................... ..........................................N..........N.......................... .......................................i........................................ .......................N..............................i......................... .......................N........................................................ .....................N.......................................................... ...................N............................................................ ......................................N.....N................................... ........................................................N....................... ................................................................................ ....i........................................................................... ............................................i............N...................... ....................................................N........................... ..................................................N............................. ..N.................N............................................i......N....... .......N.......................................................................N .................................................i.........N.............i...... .................................N.................N............................ ................................................................................ .............i.................................................................. ................................................................................ ...........N.......................................i..............i............. ............N.......N........................................................... ....................i...........................N............................... .............................................................i........N......... .........................i.........N..N..N...................................... ...............................................N................................ ............N................................................................... ......................... Pos Score Pred ------------------------ 54 0.342 - 123 0.485 - 134 0.242 - 162 0.592 Yes 186 0.637 Yes 280 0.514 Yes 344 0.120 - 375 0.810 Yes 424 0.385 - 502 0.135 - 580 0.110 - 679 0.199 - 685 0.098 - 777 0.476 - 885 0.635 Yes 1005 0.768 Yes 1018 0.380 - 1093 0.481 - 1171 0.209 - 1203 0.332 - 1221 0.461 - 1266 0.748 Yes 1273 0.213 - 1288 0.145 - 1360 0.359 - 1410 0.706 Yes 1420 0.273 - 1434 0.640 Yes 1474 0.220 - 1492 0.202 - 1614 0.641 Yes 1772 0.158 - 1812 0.719 Yes 1827 0.549 Yes 1853 0.125 - 1861 0.196 - 1941 0.616 Yes 1969 0.300 - 2062 0.584 Yes 2071 0.208 - 2106 0.693 Yes 2116 0.206 - 2119 0.120 - 2122 0.150 - 2208 0.306 - 2253 0.130 -

Explain the output -- Processing locus 103: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAACAGAGGAAATTCATTTAACAGTCTAATAGTCTTACCGAATTTACCAAAGTATTTTTAACATAAGTTAAAACATTTGT GATTTTGCTTGTTTGTTATGACTTCTTATAAGTTCTAATTGTATGTATATTAGATCATCCCCATCGGAGAATCTTCTCCT CCATAGGAAAATGTCGTCTAATTCCCTGAGGCATGTCGAGTCCATGTCCCAGCTACCCTCAGGTGCCGGAAAGATCTCTC AATTAAACGCCGTCGTGTTAGGAGAATCCCTTGCATCGGAGGAGAATGATCTCGTCTTTCCCAGCAAGGAATTCTCCGGC CAGGCTCTTGTTTCCTCCCCTCAACAGTACATGGAAATGCATAAGAGGTCGATGGATGACCCTGCTGCTTTTTGGTCTGA TATTGCGTCTGAGTTTTACTGGAAGCAGAAATGGGGTGACCAAGTGTTTTCCGAGAATCTCGATGTCAGGAAGGGTCCTA TTAGCATCGAGTGGTTTAAAGGGGGAATCACCAATATTTGCTACAACTGTTTGGACAAAAACGTTGAAGCTGGTTTGGGT GATAAAACAGCCATACACTGGGAAGGAAATGAACTTGGGGTAGATGCTTCCTTAACTTATTCTGAGTTGCTCCAACGAGT TTGCCAGCTTGCTAATTACTTGAAAGATAATGGAGTGAAGAAGGGTGATGCTGTTGTAATTTATTTACCTATGTTGATGG AACTTCCCATCGCTATGCTTGCATGTGCAAGGATCGGAGCTGTTCATTCGGTCGTTTTTGCTGGATTTTCTGCGGACTCT CTTGCCCAAAGAATCGTTGATTGCAAGCCAAATGTAATACTGACTTGCAATGCTGTTAAAAGAGGCCCTAAGACCATAAA CCTTAAAGCTATTGTTGATGCTGCACTTGACCAATCTTCTAAAGATGGAGTCTCTGTAGGCATATGCTTGACCTATGACA ACTCATTAGCGACAACAAGGGAAAACACTAAATGGCAGAATGGAAGAGATGTGTGGTGGCAGGATGTTATTTCTCAATAT CCAACATCGTGTGAGGTGGAATGGGTTGATGCAGAAGATCCCCTGTTTTTGCTCTACACCAGTGGAAGTACTGGGAAGCC AAAGGGTGTCCTACACACAACTGGAGGGTATATGATCTACACTGCTACAACGTTCAAATATGCATTTGACTACAAATCAA CAGATGTATACTGGTGTACAGCAGATTGTGGTTGGATAACTGGCCATAGCTATGTTACTTATGGACCAATGCTTAATGGA GCCACTGTTGTTGTCTTTGAGGGGGCTCCAAACTACCCTGACCCTGGACGCTGTTGGGATATTGTTGACAAATACAAGGT TTCAATATTTTATACTGCCCCAACATTGGTGAGGTCTCTCATGCGCGATGACGATAAGTTTGTAACACGTCACTCGCGCA AATCGCTGCGGGTCCTTGGAAGTGTTGGTGAGCCCATCAATCCTAGTGCCTGGAGATGGTTCTTCAATGTAGTCGGTGAT TCAAGGTGTCCCATTTCAGATACGTGGTGGCAAACTGAAACTGGTGGCTTCATGATAACCCCATTGCCAGGTGCTTGGCC CCAGAAACCTGGTTCAGCCACTTTCCCTTTCTTTGGTGTTCAGCCTGTCATAGTTGATGAAAAGGGCAATGAAATCGAAG GCGAGTGTAGTGGTTATCTTTGTGTCAAAGGTTCATGGCCCGGGGCGTTTCGAACTCTGTTTGGGGATCATGAAAGATAC GAAACCACATACTTTAAACCTTTTGCCGGATATTATTTCAGTGGTGATGGTTGCAGCAGAGACAAGGATGGTTACTACTG GCTTACAGGGAGGGTTGATGATGTTATCAACGTCAGTGGCCATCGAATCGGAACTGCTGAAGTAGAATCTGCTCTGGTTT TACACCCTCAATGTGCAGAAGCAGCTGTTGTAGGCATAGAACATGAGGTAAAAGGTCAGGGAATTTATGCGTTTGTCACT CTTCTGGAAGGTGTTCCTTACAGCGAGGAGCTTCGTAAAAGCCTAGTACTAATGGTCCGAAATCAGATTGGGGCTTTTGC AGCACCGGACAGAATACATTGGGCACCAGGGTTGCCAAAGACGAGAAGCGGAAAGATAATGAGGAGAATCTTGAGAAAGA TTGCTTCGAGGCAATTAGAAGAACTCGGAGATACTAGCACACTCGCGGATCCTAGTGTAGTTGATCAGCTTATTGCACTT GCCGATGTGTGATGACTAACCATAGCATATGAGCCAATGGAGAGTAATGGAGTTTTGTGCATCTATATATGTTTTCAGGT GTCTTATAGAGGGAATGGTACAAAAATCTGA ................................................................................ .................N........................N..................................... ..........i.....................i..........N.................................... .............................................N.................................. ..............................N.....i..............i...N........................ ..............................N...............................N................. ................................................................................ ............................N..............N.................................... .............................N.................N......................i.....i... ..............i.......N......................................................... ...............................N.................N.............................. .................N..........................i..................N..........N..... ...............................i.......i........N..............N................ ....................i.......N................................................... ...............................N...........................N.................... ...N...............................................N........N.......N......N.... ................................................................................ ........................................i......i................................ .......................................................N..........N............. ...................................................N............................ ........................................................N...........i........... ..................................N..................................N.......... ..............................................i....................N............ .................N..N........................................................... ..........N...............................N.......................N............. ...................................................i............................ ..........................................................N..................... ................................................................................ ....N......N................N.......N.........N.....................N........... ..............N................ Pos Score Pred ------------------------ 98 0.398 - 123 0.227 - 171 0.660 Yes 193 0.610 Yes 204 0.347 - 286 0.281 - 351 0.345 - 357 0.630 Yes 372 0.815 Yes 376 0.442 - 431 0.451 - 463 0.197 - 589 0.249 - 604 0.096 - 670 0.240 - 688 0.128 - 711 0.503 Yes 717 0.743 Yes 735 0.525 Yes 743 0.150 - 832 0.161 - 850 0.339 - 898 0.304 - 925 0.610 Yes 944 0.300 - 955 0.165 - 992 0.704 Yes 1000 0.566 Yes 1009 0.418 - 1024 0.208 - 1061 0.735 Yes 1069 0.220 - 1152 0.393 - 1180 0.495 - 1204 0.251 - 1252 0.373 - 1261 0.144 - 1269 0.495 - 1276 0.160 - 1401 0.573 Yes 1408 0.505 Yes 1496 0.410 - 1507 0.276 - 1572 0.382 - 1657 0.310 - 1669 0.778 Yes 1715 0.304 - 1750 0.222 - 1807 0.687 Yes 1828 0.342 - 1858 0.144 - 1861 0.321 - 1931 0.296 - 1963 0.246 - 1987 0.259 - 2052 0.562 Yes 2139 0.492 - 2245 0.208 - 2252 0.187 - 2269 0.075 - 2277 0.119 - 2287 0.163 - 2309 0.039 - 2335 0.039 -

Explain the output -- Processing locus 104: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGACTCGTCGACCTCTGATCCGCTTCTTCGACTATACGGATCTACTTCCGATCTGTAAGAAGCTTAAATCTTCGGTTGAT TTTGGAATCCATCCGTCGTAGATGTGTAATTGTATTGATTTGGATGAGTTTATCTGTTTTTTTGAGTTTTCTTATGCAGT GATGATGGCCGGAGGTAACGTACGGTTTCTGGTTTTGATACTCGCCGTAGCAATTTGCGGTGCTGCTTCTGTTTTCCAGC CGATCTCAGACTCTCACCGATCTGCGGCTCTGGATGTCTTCGTACCAGTCGATGGATCATACAAAAGCTTGGAGGAGGCA TATGAAGCATTAAAGACTTTGGAGATTCTTGGAATTGATAAAAAGTCTGATCTTAGCTCAAAGACTTGTGAGAATGTGGT TAAAGTTCTTCAGTCGTCTTCTTCTACTCTTAAGGATGCATTTTATGCGTTAAACGTTAATGGGATTCTAAAATGTAAAA TTGGTGAAGCTGGTCCTAAGGACATTGTTTCTCAACTTCAAGCTGGTGTTAAAGATGCGAAGCTATTGCTTGATTTCTAT TATTCTGTCAGAGGCTTGGTGCTGGCTAAGGAGCAATTTCCTGGAACTCATATAAGTCTTGGAGATGCCGAAGCCATTTT CCGTTCCATCAAGTCTCTTAGCCAGAGTGATGGAAGATGGCGCTACAGCTCCAACAATCCCGAATCAAGCACTTTTGCTG CTGGCTTAGCCTATGAAACGCTTGCTGGAGTGATTTCATTAGCACCTTCAGAGTTTGATCCATCTTTGATCCAGTCGGTG AAGACGGGTATCCTGAAGCTTTCTGACAGTATCCAAAAATATGATGATGGGACATTTTATTTTGATGAGAAGTCTGTTGA TGCAAGTCAAGGCCCGATCTCAACAACTGCATCAGTTATTAGAGGGCTCACGTCATTTGCAGCTTCAGAATCCACAGGAT TGAACCTTCCAGGTGATAAGATAGTTGGTCTGGCCAAATTCTTTCTGGGTGTTGGAATTCCTGGTGATGCCAAGGACTTC TTCAACCAAATAGATGCACTAGCTTGTTTGGAAGACAACAAGTTTTCTGTTCCACTCATCTTGTCTCTTCCATCCACTGT CATTTCATTGACAAAGAAAGAGCCTCTGAAGGTTAAAGTTAGCACTGTACTGGGTTCGAAGGCACCAGCTCTTAGTGTGA AGCTTACACAAGCTCTAAGTTCTAAGTCAGTGGATTCATCTGTAATTAATAACCAGGAGCTTAAGTTTGACGCTGACAGT GCAACGTACTTCCTGGACTCCTTTCCCAAGAACTTTGACATTGGAAAATATACTTTTGTATTTAAGATTGTGCTGGATGA ATCAGCACATGAGAAAGTATACATAACTGAAGCTCAAACAAAAGTGCCTATAGCTGCCACAGGAGCTATTAGCATTGAAA ATGCAGAAATTGCTGTACTTGACAGTGACATTGGAAGCGTTGAATCTCAGAAAAAGCTAGATTTAACTAAAGATGGAGCG GTATCATTATCAGCAAACCACCTTCAAAAGCTACGCCTGTCATTCCAGTTAACTACTCCACTTGGCAATGCGTTTAAGCC ACACCAGGCATTTTTCAAGCTGAAACATGAGTCACAGGTCGAGCATATCTTTCTCGTGAAAACTTCTGGAAAGAAGTCTG AATTAGTTCTAGATTTTCTTGGATTGGTTGAGAAGCTCTACTACCTCTCTGGTAAATATGAGATACAGCTAACCATTGGA GATGCTTCTATGGAGAACTCTTTACTGAGTAATATTGGTCACATTGAACTAGACCTACCAGAGCGTCCAGAGAAGGCGAC TCGTCCTCCTCTACAGTCTACTGAGCCTTACTCAAGATATGGGCCAAAAGCTGAAATATCACACATATTCAGGATTCCTG AAAAGCTTCCAGCAAAGCAGCTCTCACTAGTTTTCTTGGGTGTTATCGTTCTCCCATTCATCGGTTTCCTGATTGGGCTT ACGCGGTTGGGAGTGAACATAAAGAGCTTCCCATCGTCGACTGGATCTGCAATATCCGCTTTACTTTTCCACTGCGGAAT TGGAGCTGTTTTGCTTCTATATGTGCTATTCTGGTTGAAGTTGGATCTATTCACGACTCTCAAGGCACTTTCTTTGTTGG GAGTGTTTCTGTTGTTCGTGGGACACAGAACACTATCTCAACTTGCATCAGCATCAAACAAGTTGAAATCTGCTTGAAGA AGAAGAACTCACTATGAAGGATCTTTTTTTGGTTTATGCGTATTTTCAGTTTTGTAGTATTGCTTATAGATTATGGTGAT AGACAAATTCAAAAGA ................................................................................ .....................i.....................N.............................N...... .................................N.................N............................ ...................................N........N..............N............N....... ......................................................N......................... ................................................................i............... .............................N......N........................................... ............N................................................................... ........................................N..N..N.................N..............N ................................................................................ ..................................................................N............. .............N.................................................................. ................................................................................ ................................................................................ ............................................................................N... ........N....................................................................... N.......................................................................i....... ...................................................................N............ ..........................i..................................................... .........................................................N...................... ......................................N......................................... ................................................................................ ................................................................................ ....................N........................................................... ................................................................................ .............N.....................N....................................N....... ................ Pos Score Pred ------------------------ 102 0.524 Yes 124 0.102 - 154 0.239 - 162 0.575 Yes 165 0.818 Yes 274 0.172 - 292 0.300 - 322 0.066 - 394 0.130 - 436 0.222 - 445 0.116 - 460 0.092 - 473 0.101 - 535 0.217 - 625 0.807 Yes 670 0.450 - 677 0.399 - 733 0.118 - 841 0.305 - 844 0.236 - 847 0.405 - 865 0.197 - 880 0.217 - 1027 0.163 - 1054 0.140 - 1357 0.246 - 1369 0.413 - 1441 0.306 - 1513 0.597 Yes 1588 0.317 - 1627 0.622 Yes 1738 0.239 - 1762 0.374 - 1770 0.544 Yes 1879 0.277 - 2101 0.293 - 2254 0.144 - 2276 0.033 - 2313 0.053 -

Explain the output -- Processing locus 105: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGACCAAAAAAAAAAAAAAAAAAGGAGGGGTTAATATTGACGATTTTGATAATGAGAGACTTTTAACGTAATTTATAATA ATTACCGACGTTGATTTGTAAATCCATAAAGTTACAAAAAAATCTCAAATGTGGGAACAGCACAGAAGCGAGCAACTTCA CCGCACAAAAATGCATACTCTCAACAACACCATCACCACCACCACCGGTTCGCAAGATCCGAATCTCGACCCAATTCCAG ACCCGGATCAATTTCCCAACCGGAACCGCAACCAACCACAATCTCGCCGCCCTCGCGGATTCGCCGCTGCAGCAGCAGCA GCGTCGATAGCTCCAACGGAAAACGATGTAAACAACGGAAACATCGCCGGAATCGGCGGTGGAGAAGGAAGCAGCGGCGG CGGAGGAGGTGGAGGAGGGAAAGGGAAGAGAGAGAGGGAGAAGGAAAAAGAGAGAACGAAGCTTAGAGAAAGACATAGAC GAGCAATCACTAGTAGAATGCTCGCCGGATTAAGACAGTACGGTAATTTTCCGTTACCGGCGCGTGCTGATATGAATGAC GTAATCGCTGCTTTGGCTCGTGAAGCTGGTTGGAGTGTTGAAGCCGATGGTACTACTTATCGGCAATCACAGCAACCAAA TCATGTGGTACAGTTTCCGACGAGATCTATTGAGAGTCCACTTTCTTCTAGTACATTGAAGAACTGTGCTAAGGCTGCAA TTGAATCTCAGCAGCATTCAGTTCTTAGGAACGATGAGAAACTTGCTCCTGTGTCTCTTGATTCTATTGGTATTGCCGAG AGTGATCATCCCGGGAATGGAAGGTATACTAGTGTTAGTCCTATCACCTCCGTTGGATGCTTGGAAGCCAATCAGCTTAT ACAAGATGTTCATTCCGCAGAGCAATGTAATGACTTCACAGAGAGCTTTTATGTCCCTGTGTACGCCATGCTTCCAGTTG GCATTATAGACAACTTTGGCCAGTTGGTTGATCCTGAAGGTGTAAGACAAGAATTAAGTTACATGAAGTCATTAAATGTC GATGGAGTAGTAATAGATTGTTGGTGGGGAATAGTTGAAGGATGGAACCCTCAGAAATATGTATGGTCTGGCTATAGGGA GCTCTTTAACCTTATCAGAGACTTCAAACTTAAGCTACAGGTGGTGATGGCATTTCATGAGTATGGAGGGAATGCATCTG GGAATGTGATGATTTCGTTGCCACAGTGGGTGTTGAAGATTGGGAAAGACAATCCAGACATTTTTTTCACTGATCGTGAA GGAAGAAGAAGTTTCGAGTGTTTGAATTGGAGCATTGACAAGGAACGGGTATTGCATGGACGAACTGGTATAGAGGTCTA TTTTGATTTTATGAGAAGCTTCCGGTCAGAGTTTGATGACTTGTTTGTGGAAGGACTAATTACTGCGGTTGAGATTGGTC TTGGAGCTTCTGGTGAACTAAAGTACCCATCTTTCCCAGAAAGGATGGGTTGGATTTATCCCGGTATCGGTGAGTTTCAG TGTTATGACAAATATTCGCAATTGAGTCTGCAAAAGGAAGCAAAGTCACGAGGATTCACTTTCTGGGGTAAAGGACCAGA GAATGCTGGTCAATACAGTTCTCATCCACATGAAACTGTGTTCTTCCAAGAAAGAGGTGAATATGACAGCTATTATGGCC GCTTCTTCTTAAATTGGTACTCACAATTGCTAATTGGTCATGCTGAGAATGTTCTGTCTCTTGCAAATCTCGCATTTGAG GAGACGAAGATTATTGTCAAGATACCGGCAATATACTGGTCATACAAGACAGCTAGTCATGCTGCTGAATTAACTGCAGG ATACTACAACCCTTCAAACCGTGATGGGTACTCTCTTGTTTTCGAAACTCTGAAGAAATATTCCGTGACTGTGAAGTTTG TGTGCCCTGGGCCACAAATGTCTCCCAATGCGCATGAAGAAGCATTAGCTGATCCAGAAGGTTTAAGTTGGCAGGTGATC AACGCAGCTTGGGATAAGGGACTTCAAATCGGTGGAGAGAATGCGATTACTTGTTTCGACAGAGATGGTTGTATGAGATT AATCGATATAGCTAAACCAAGAAACCATCCAGATGGCTATCACTTCTCCTTCTTCACATATCGTCAACCTTCTCCTCTGG TTCAAGGATCTACTTGCTTTCCAGATTTGGACTACTTCATTAAACGCATGCATGGCGACATAAGAGATAAACAGTTTTAG GATTTGCATGGTCTTAGGATTCTTTTAGTAAGTGTCTTTTGTTTAAAACTTCAAAATCTGTTTTGTTTTGTTTATTCTCT CCTCAAGGCCTATATTTAA ...................................................N............................ ................................................i............................... ..........i..................................................................... ................................................................................ .........................i...................................................... ................................................................................ .................i.....................................................i...N.... ..............................................N................................. ..N............................................................................. .................................N.............................................. ................N.......................................N....................... .....N..................N....i....................N................N............ ..............................................................N............i.... ..............................................N.........N.....N........N........ ...N....N....................................................................... .......................................................N........................ ..........i........................N............................................ ............................................N................................... ....N........................................................................... ..i..........................N................................i...........N..... .......................................N........N............................... ..........................................................N..................... .......................N........................................................ .................i.........N.....N.............................................. ........................................N.......................N.......N....... ................................i............................................... ...............................................N...N............................ .......N........................................................................ ................... Pos Score Pred ------------------------ 52 0.292 - 129 0.788 Yes 171 0.858 Yes 346 0.856 Yes 498 0.625 Yes 552 0.787 Yes 556 0.244 - 607 0.118 - 643 0.123 - 754 0.226 - 817 0.136 - 857 0.193 - 886 0.122 - 905 0.278 - 910 0.628 Yes 931 0.235 - 948 0.476 - 1023 0.421 - 1036 0.545 Yes 1042 0.412 - 1082 0.207 - 1099 0.271 - 1103 0.608 Yes 1167 0.368 - 1177 0.213 - 1183 0.195 - 1192 0.263 - 1204 0.271 - 1209 0.296 - 1336 0.247 - 1371 0.592 Yes 1396 0.095 - 1485 0.378 - 1525 0.250 - 1603 0.616 Yes 1630 0.121 - 1663 0.637 Yes 1675 0.317 - 1720 0.273 - 1729 0.310 - 1819 0.303 - 1864 0.206 - 1938 0.689 Yes 1948 0.344 - 1954 0.207 - 2041 0.198 - 2065 0.307 - 2073 0.189 - 2113 0.695 Yes 2208 0.235 - 2212 0.268 - 2248 0.091 -

Explain the output -- Processing locus 106: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACATTTCTGAGTTAGTCTGTGGTTTTTTATAAAAACTTTAATTGACTCAGTCCCATCATAAAGTGGGACATCCATTTCA TAACATTTCATTTTGGACTAAAGTTTGGTTCCTTTGAAATTTGGGAAACTGAAATGTATGAATATGTCTAGCAGGAATTC AAGTGCACAGGCAGGTGTTGGTTCTGGTGCTTTATCACATGTCTATATACATCATCCATCCCTACGTTGCAATATTCCTG AATCCAATGGGTTATTCTATGATGATGCCAACAGATTGCTAATATGTACCACATCTAGTCAGGTCTTTTCATGGGAAACA TCCCCCTTTAATCCAGACGTACCTCCTTCAGTTGATTCTATAAGTGAAGGGCCTATTCTATCAATCAGATTTTCTCTTGA TAAAAAAGCGATAGCTGTCCAAAGATCTGACTGTGAAATACAGTTGTTTAATCGAGAGACCAAGCAGATACTTAACCACA AATGCAAGGCAGGATCAGAGAGCATTCTCGGATTCTTTTGGAGTGACTCTCCATTATGTGATCTTGCAATTGTAAAGACC AGTGGTATGGACTTGTTCGCTTGTGATTCTATGTTGAATTCACTACGTTTGGTGGAAACCAAAAAGGCGAATGTGAATTG GTACATCTATACACATGAAACTCGATTGGTTCTTCTTGCATCAGGACTGCAATGCAAGACATTTAATGGATTTCAGCTTT CCACTGCAGGAGTTGTTCGTTTACCGAGATTTGAGATGACAATGGCTAGATCTGAATCAAACAGTAAACCTATTCTCTCT GCTGGAGATCTCCACTTAGTCACTGTCTATGGCCGAATATATTGCTTACAAGTGGACAGGGAGGCAATGCTACTGCATCT ATACAGGTTCTACCGTGATGCAGTCGTCCAACAAGGTTCTTTACCAATATACTCAAGCAAGTTGTCGGTGAATGTGGTCG ACAATTTATTGCTTGTGCACCAAATCGATGCAAAGGTTGTCATCATCTACGACTTATTTGTGGATTCTCGAGCTCCTGTA TCTGCGCCTCTTCCTTTACTGTGGAGGGGTTACCAAGTCTCTGAGACATCGTCTCAAGCTGTTAACAAAGAAATTGAGAG CTCAGAATCATCTACAAGCAATGAAAACATAGTCATGTATGAAGATGGCTGGACCTTTCTGGTTCCAGACCTTATTCTGG ATCAGACAAACAAGGTTTTATGGAAGATACATTTGGATCTTGAGGCAATTTCCGCTAGCAGCTCAGACAGGACGTCTCTT CTAGAATTCCTGCAGCGAAGGAAGTTGGAAGCAAATAAGGCCAAACAATTGTGCTTGGGGATAGCAAGGGCTATTATTTT GGAACGTCGACCAGCAACCCAGGTAACTCAGGCAATCGATGTACTAGTCACAGCATATTCCTACTCGGTTAAAGCCGGTA CATACAAGGAAATAAAGAACGAAAACGCCACTGCATCTACTCCAACCCCTGGTGCATCTCCTGATAGCGAAAGACATAGG CAGTCTGGAAGCAATATTGATGAAGAAGTTGAGATGAACCTATCATCTGGTTCTCAGGAGAACATGTTTTGTGCTGATGA ACAGCAAGAATCTCAACTCTCTTCACCGGCAATTTCACCCGATGAGCTCTACAAGTTTGTATTTACTTCTGTGGAGGAAA TGATGGTTGAGGAATCAGAGTACCTAGTTGCTATCATCACCGAGTTCCTTCGCAGTATAAGTGCAGAAAAACTCAAAGTG GATTTGAACATCTATGTGATGACCATCAAACTTTTAGCTTACAGTAAGCGATTTGCAGAACTGTCGCTATTCACCACAAA CAAGATAATCGAACCATCAAAGGAAGTCGCGTTCCAACTTCTGGATAGTGGTCGTCAAAATATCCGGGTAAGAAAACTTG GGCTTGATATGTTGAGACAGCTCTCCTTGCACCATGATTACATCTCCTCCCTTGTGCAAGATGGATACTATCTTGAAGCT CTGCGATATGCTCAGAAGCATAAGGTGACGAGTGTGCGATCGTCGATGTTCCTAGAAGCAGCATTCGCGTCGAATGATAT GCAGCATTTGGCTGCAATTTTGAGGGTCTTGTCGGAATTGATTCCCGGGTTTAAAGAAACTTCTGAATATTACACCTTTT ATGGTCTTCTCAATGACACCAGTTCCTCAGTTGCTGTCTGAAGAGAATCTCATACCACATTTTATGTTCAAGAAAGTAAA ATGATCTCTGGTTCTTCGACTGGATCTTGATAAATAAATGCTGTTGAACATTTTCTGGTT ................................................................................ .....................................................N...N.....i................ ......................................N......................................... ......N...........N..N..N..................N..........................N......... ................................................................................ ................................................................................ ......N.......................i.......................................N......... ..............N....................................N.............N.............. ...................................i.....i...................................... ............................N.....................................N............. .................N.....................................................N........ ...........................i.................................................... ................................................................................ ....................N.............i...N.....i................................... ...................i............................................................ ................................................................................ ......................................N......................................... ................................................................................ ...................N.............i.............................N............N... .........................................N.....................................N ..i............................................................................. .............N....N............................................................. ................................................................................ ........N........................i..........................N................... .......N.....................................N...........................N....N. ................................................................................ N...........N..................................................N................ N....................................N...................... Pos Score Pred ------------------------ 134 0.234 - 138 0.379 - 144 0.538 Yes 199 0.218 - 247 0.426 - 259 0.296 - 262 0.417 - 265 0.268 - 284 0.387 - 311 0.179 - 482 0.172 - 536 0.413 - 567 0.432 - 591 0.524 Yes 631 0.385 - 655 0.157 - 692 0.328 - 706 0.409 - 756 0.594 Yes 762 0.606 Yes 829 0.329 - 867 0.349 - 898 0.323 - 952 0.268 - 988 0.566 Yes 1141 0.161 - 1155 0.727 Yes 1159 0.299 - 1165 0.512 Yes 1220 0.593 Yes 1399 0.398 - 1540 0.326 - 1554 0.546 Yes 1584 0.340 - 1597 0.149 - 1642 0.099 - 1680 0.481 - 1683 0.775 Yes 1774 0.272 - 1779 0.294 - 1929 0.302 - 1954 0.543 Yes 1981 0.221 - 2008 0.126 - 2046 0.178 - 2074 0.210 - 2079 0.461 - 2161 0.209 - 2173 0.341 - 2224 0.059 - 2241 0.161 - 2278 0.056 -

Explain the output -- Processing locus 107: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAATTTTTTAATCTTATTCTTCTTCAGGTGCTATGGTACGAAACTGGATTCAGAAAGAGGTCGAGCAAAAAGTCAAACA GACGACTCTACAGAAATATAAGAAACGAACCAGACGAAACTGTATATGTTAGGACAAGAAGCAATGATGAGAGTTTCTCA ACTCCAACTCTGTCGCACTTCTCTTTCGTACCGTCTTCGCTCTGGTTATCATCATCATCATCATCTTCACCACAGTTTCT TCAAGCTCATCAAACGTAACTCAATTCTTGAATCTCCACCTACTAATGCATCTCATCAATCTCCATCCCCGATTACTCCT ATGGTCAACGCACGCGTCATGTTCTTCTCCACTTCAACTTCAGCTCCACATCCAGAGAAAATCAACAGGACGTCGTCGGA GAATATCCTTCGGATGATTTCAAGCTATCTATGGATGAAAGACAACCCAAAACTCTGTTTCAGAGTCATCTCTGCTTTTG CGTGTCTCGTGGGAGCTAAGTTCTTGAATGTTCAAGTTCCTTTCCTCTTCAAAGTAGCTATAGATTGGCTCTCTTCATCT TCATTCGTTGATTCAAACCCTTACCTAGTTGCTGCTTTCGCTACTCCTTCCTCTGTTCTCATTGGTTATGGCATTGCTCG ATCTGGTTCTTCTGCTTTCAACGAGCTTCGTACTAGTGTTTTCTCCAAAGTAGCTCTCCGCACCATACGAACCATCTCTA GAAAAGTTCTCTCGCGTTTACACGATCTCGATCTTCGTTACCATCTAAATAGAGACACAGGTGCTTTGAACAGAATCATT GATCGTGGAAGCCGCGCTATAAACACGATTCTCTCAGCTATGGTTTTCAACATTATGCCCACGATCTTGGAGATATCAAT GGTCTCATGTATATTAGCATACAAATTTGGAGCTGTTTATGCATTGATTACTTGTCTTTCTGTTGGATCATATATAGCAT TCACGTTGGCTATGACGCAGTGGAGAATCAAGATTAGAAAAGCTATGAATGAAGCTGAAAACGATGCTAGCACCAGAGCC ATTGACTCTCTCATAAACTACGAGACTGTTAAGTATTTCAACAACGAAGATTACGAAGCTCGAAAGTACGATCAGTTACA CGAAAATTACGAAGATGCTGCATTGCAAAGTCGGAAAAGCTTTGCCTTATTAAATTTTGGACAGAGTTTTATATTTAGTA CAGCTTTGTCAACAGCAATGGTGTTGTGTTCTCAGGGGATTATGAATGGTCAGATGACAGTTGGTGATTTGGTGATGGTG AATGGTCTCCTCTTTCAACTGTCTCTTCCTCTTTACTTTCTCGGGGTTGTTTACAGCGACACCGTTCAGGGACTTGTCGA CATGAAATCGATGTTTAAGTTTCTAGAGGAGAGATCTGATATCGGAGATAAAGATATAGACAGGAAGCTCCCTCCTCTTG TTCTGAAAGGTGGAAGCATTAGTTTTGAGAATGTACATTTTAGTTATCTACCGGAGAGAAAGATTTTAGATGGGATTTCA TTTGAGGTACCGGCAGGGAAAAGTGTGGCGATTGTCGGTAGTAGCGGAAGCGGAAAATCGACTATTCTTAGAATGATATT CAGGTTCTTTGATGTAGATTCCGGAAATGTAAAAATTGATGGTCAGGATATAAAGGAAGTGAGACTAGAGAGTCTTAGAA GCTCCATTGGAGTTGTCCCTCAAGATACTGTGCTCTTTAACGACACTATATTTCACAACATTCACTATGGAAACCTTTCA GCAACAGAGGAAGAGGTGTACAATGCAGCTCGACGAGCAGCGATTCACGATACGATCATGAAATTCCCTGATAAATATTC GACCGCGGTGGGAGAAAGAGGGCTGATGCTGAGTGGTGGAGAGAAACAGAGGGTGGCACTTGCTCGTGCATTTCTGAAAT CACCAGCAATCCTATTGTGTGACGAAGCAACAAGTGCACTCGACAGTAAAACCGAGGCAGAGATAATGAAAACGCTGAGG TCGCTAGCGAGTAACAGGACTTGCATTTTCATTGCACATAGACTGACCACTGCAATGCAATGTGATGAGATATTAGTAAT GGAGAAAGGAAAAGTGGTGGAAAAAGGGACACACGAAGTGTTGTTGGGGAAATCTGGCAGATACGCTAAATTATGGACAC AACAAAACAGTAAATTGGAGGTGTGAATTTTGTAAAACTATGAATCAAAAAGCCACTTTGGGCCTTTATATAGCCTTTTA AGAGAGTGAAAGAGCAGAAAATATAGACACCAGAAAAAATTTAAT .................................i.............................................. .............................................N.................N..N............. ................................................................................ .............................................N.................................. i.................N............................................................. .............N................i...N............................................. ...........................N.................................................... ...................................................................i............ ................................................................................ ................................................................................ .......................................N..............N.......................i. ......N...............................N......................................... ...........i................................i...i..............N................ ................................................................................ ..............N................................................................. .................N.......................i...N.......N....................N..... ..............................N......................................N.......... ........................................................................i....... ...........N..............N...........N......................................... ..................................................................N............. ......................N..................................N...................... .........................N...................................................... .................................................................i.............. ......................................................N....i....N.............N. ........................................................................N....... .......................................N........................................ ............................................. Pos Score Pred ------------------------ 34 0.534 Yes 126 0.293 - 144 0.261 - 147 0.362 - 286 0.491 - 321 0.599 Yes 339 0.409 - 414 0.355 - 431 0.642 Yes 435 0.172 - 508 0.144 - 628 0.510 Yes 840 0.293 - 855 0.186 - 879 0.538 Yes 887 0.305 - 919 0.150 - 972 0.593 Yes 1005 0.819 Yes 1009 0.611 Yes 1024 0.467 - 1135 0.235 - 1218 0.185 - 1242 0.512 Yes 1246 0.319 - 1254 0.416 - 1275 0.267 - 1282 0.102 - 1362 0.616 Yes 1371 0.492 - 1471 0.292 - 1510 0.157 - 1593 0.511 Yes 1612 0.159 - 1627 0.253 - 1639 0.275 - 1747 0.267 - 1783 0.441 - 1818 0.367 - 1866 0.463 - 1986 0.763 Yes 2055 0.347 - 2060 0.519 Yes 2065 0.237 - 2079 0.460 - 2153 0.313 - 2200 0.250 -

Explain the output -- Processing locus 108: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCATGTGTTTGTCTTCCTTTTGGTCTAGACAAACTGATAAGTTTCTTTTTGTAGCTGGTACTTTCTTTTGAGGCTTTTTT AAAAATTATCCAACGGCTTGAACTGAATTGGATTCAATGTTTCTACAAATGTCTCTTATATGTAGGTTCTTGGTTTATTT GGATGTGGTGATTATGGATATGGAGTCTTGGAAGTTGCTCAGAAGTGTTTGTGTGCTTTCTTTTCTCCTTGGCTCTTGTT TTGTGTATCAAAGCCTTCGTGTTGTTGATGCTCAAGAAGATCCAAAACCAGCCGTGACTTTGCAAGTAGATGCTTCTAAT GGAGGTGGACGACCCATTCCTGAAACGCTATTTGGGATTTTCTTCGAGGAGATCAACCATGCTGGTGCCGGTGGATTATG GGCCGAACTTGTTAGCAACAGAGGGTTTGAAGCTGGTGGGCAAAACACACCCTCTAATATTTGGCCATGGTCCATTGTTG GTGATCATTCATCCATATATGTAGCTACAGACCGTTCATCATGTTTTGAACGGAATAAAATCGCACTGAGAATGGATGTG CTTTGTGATAGCAAAGGTTGTCCATCGGGAGGTGTCGGAGTTTATAACCCTGGTTACTGGGGCATGAATATTGAAGAAGG AAAGAAGTACAAGGTGGCTCTTTATGTGCGTTCGACTGGTGATATCGATTTGTCTGTGTCGTTGACGAGCTCGAATGGAT CACGGACTCTTGCTTCTGAAAAGATCATAGCTTCTGCTTCTGATGTTTCGAAATGGATTAAAAAAGAGGTTCTTTTGGAG GCAAAAGCGACCGATCCTAGTGCAAGACTTCAGTTAACAACAACCAAGAAAGGGTCAATATGGATTGACCAAGTCTCGGC CATGCCAGTGGATACTCACAAGGGACATGGTTTTAGAAACGATCTTTTCCAAATGATGGCCGATATAAAACCCCGTTTCA TCCGGTTCCCAGGTGGTTGTTTTGTCGAGGGTGAATGGTTAAGTAATGCCTTCCGCTGGAAAGAAACCGTAGGACCTTGG GAAGAGAGACCGGGCCATTTTGGTGATGTTTGGAAGTATTGGACCGATGATGGCCTTGGCCACTTTGAGTTCTTTCAAAT GGCAGAAGACATCGGTGCAGCCCCGATATGGGTGTTTAACAACGGGATAAGTCACAATGATGAAGTTGAAACTGCCAGTA TCATGCCCTTTGTTCAAGAAGCACTCGACGGTATCGAGTTTGCCCGAGGAGATGCCAATTCTACTTGGGGATCTGTTCGA GCTAAAATGGGACGCCAAGAACCTTTTGAACTTAAATACGTTGCTATTGGAAATGAAGATTGTGGGAAAACTTACTACAG AGGAAACTACATTGTGTTCTATGATGCTATCAAGAAAGCCTATCCAGATATCAAAATCATCTCCAACTGCGATGGATCCT CTCATCCACTCGACCACCCAGCTGATTACTATGACTATCACATTTACACTTCTGCTAGCAATTTGTTTTCCATGTATCAT CAATTTGATCGCACTTCACGCAAAGGTCCAAAGGCTTTTGTCAGTGAATATGCTGTTACTGGAAAAGATGCTGGTACAGG AAGCCTTCTTGCCTCTCTTGCAGAAGCTGCGTTTCTCATTGGTCTTGAAAAGAATAGTGACATTGTTGAAATGGCAAGTT ATGCTCCACTCTTTGTGAACACAAATGATAGACGGTGGAACCCTGATGCAATAGTCTTCAACTCCTCTCATCTATATGGA ACACCTAGCTATTGGGTTCAACGGTTTTTTGCAGAGTCAAGCGGAGCAACTCTTCTCACTTCAACACTCAAGGGAAACTC TACTTCTCTTGTTGCATCTGCTATCTCTTGGAAAAACAATGGCAAAGATTACATACGCATAAAGGCTGTAAACTTCGGAG CTAACTCAGAAAATATGCAAGTGTTGGTAACTGGATTGGACCCGAACGTGATGAGAGTTTCGGGATCAAAGAAGACAGTA CTTACCTCTACCAATGTGATGGATGAAAATTCCTTCTCACAGCCAGAGAAGGTTGTACCACATGAAAGCTTGCTAGAGTT GGCAGAGGAGGATATGACCGTTGTTCTCCCGCCACATTCCTTTTCTTCATTCGATTTGTTGAAGGAATCTGCAAAGATAA GAATGCCAATCTCTGATTCTTCTTCCCATCAGAAAACTACCACTGTGTGAAGAGAAACAATGACAAGGAAGTATCAACTT TCATGTCAAAAGTCTCTTTGTGGTTCAAATCTAATTACATTCCATAAATGCTTATAACGTACCCTTGTG ..N............................................................................. ....................................N...........i..........N.................... ..N..........N.....N............................................................ ...........................N.........................................N........N. ..........................................................N..................N.. ..................................................................N............. ..................i.....................N..............................i...i.... ...............................................................i................ .......................N..................................................N..... ..........................................N.........i........................... ...........................................................i.................... ..................................N..........N.................................. .........................N....................N..N............................i. ...........................N............................N..N.................... ..i................................................N............................ ......i.............................................N........................... ....................N..N...............................................N........ ..............................N........................................i........ .................................................N.................N............ ......................................................................i......... N.......................N....................N.............................N.... ................................................................................ ......................................N......................................... ..............i...................................N............................. .............N....N...N......................................N.................. .............N.................................................................. ..N........................................................N.................... ..N............................................N..................... Pos Score Pred ------------------------ 3 0.214 - 117 0.335 - 129 0.572 Yes 140 0.188 - 163 0.159 - 174 0.331 - 180 0.394 - 268 0.150 - 310 0.139 - 319 0.376 - 379 0.415 - 398 0.186 - 467 0.310 - 499 0.533 Yes 521 0.193 - 552 0.772 Yes 556 0.636 Yes 624 0.579 Yes 664 0.115 - 715 0.116 - 763 0.138 - 773 0.674 Yes 860 0.534 Yes 882 0.146 - 907 0.477 - 933 0.532 Yes 936 0.745 Yes 995 0.215 - 1006 0.440 - 1066 0.412 - 1087 0.215 - 1090 0.196 - 1119 0.862 Yes 1148 0.499 - 1177 0.354 - 1180 0.372 - 1203 0.542 Yes 1252 0.297 - 1287 0.700 Yes 1333 0.328 - 1381 0.299 - 1384 0.232 - 1432 0.417 - 1471 0.308 - 1512 0.788 Yes 1570 0.229 - 1588 0.413 - 1671 0.645 Yes 1681 0.200 - 1705 0.463 - 1726 0.340 - 1756 0.179 - 1879 0.312 - 1935 0.739 Yes 1971 0.233 - 2014 0.448 - 2019 0.428 - 2023 0.093 - 2062 0.243 - 2094 0.435 - 2163 0.386 - 2220 0.124 - 2243 0.077 - 2288 0.058 -

Explain the output -- Processing locus 109: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAATAATGGCATAAAGCAGACGCATTAACTTTTGTAATTGGGGTTTTACCTTTCGTTTTCACTTTAATTATTTTAACTAA TCCTTTTTACAAGACCCATAAAGAACAAAAGTCAAAATAATCGATAGTCTCAAAAAGTCAAGAATCTCTCACACACTCAT TATGAACACGCACCACCAAACAACACTTCCATTGATCCTCATAATAATCTCCACCAATACTCATTTCCCTCTTGGATTCT TCAAACTCCTCTCTGAAAGTTCTCATTTTTGGTTTACATTTCCTTTGAGTCCACTGTTACTTACTAAAATGGTGAGCAAA TGGAAGAAGATGAAGCTAGCTTTGAGTTTGAATCTCTGCAACTATCTCCCCAGAACCCTCGAGGAGGAACCACCATCATT GGCTTTGAATTCCGCTGAGACATTATCAGATGCTGCTTTGCTCTCTCCTCTTAACTGGCCCATGACTCCAACACCTTCTT CTTACCGTCGAAGGTTGTCCAGAAGTAGCAGCAAATCGTCAAAGACTTGCTCTATATGCTTGAACAAGATGAAAGAAGGT TGTGGACATGCAATTTTCACAGCGGAGTGCTCGCACATGTTTCATTTTCATTGCATTGCTTCGAATGTGAAACATGGCAA CCAAGTTTGCCCAGTGTGCCGAGCTAAATGGAAGGAAATCCCTATACAGAAGCCGAGTTTGGATCTTCCTTATTACCCCT TTGACAGGTGCAATAATGATGCAGCCATTAGCCTTTTCCGCTGCTTACCTCCTTCTCAACGAGCTATAACCCAAGGGCAT CCCGAGCCAGCTACTTTCGATGATGATGAACGGTTAGAAGAGCAGATTGTTTTTGATGGTGAAACTGAAGTCTTGAAGAA GGAGAACCGTGATTATGTGCGAATGATGGACATGAAAGTGTATCCAGAGGTCTCAGCTGTACCACAATCCAAGTCTTGTG AGAATTTTGATGTACTAGTGCATCTCAAAGCAGTTACTGGTGATCAGATTTCTCAGTATCGCCGAGCGCCAATTGATTTA GTTACAGTGCTAGACATCAGTGGTAGTATGGGAGGAACTAAGCTAGCACTTCTGAAACGAGCAATGGGTTTTGTGATTCA AAATCTTGGGTCCAGTGATAGGCTCTCAGTGATTGCTTTCTCTTCAACAGCTCGACGTCTCTTCCCTTTAACCCGAATGT CTGATGCTGGAAGACAGCTAGCCCTTCAAGCTGTTAACTCACTGGTTGCAAATGGTGGGACCAATATTGTCGATGGTCTT AGAAAAGGTGCCAAGGTTATGGAAGATAGGCTAGAAAGAAATTCTGTTGCCAGCATTATCCTTTTGTCTGACGGGCGAGA TACATATACTACGAACCATCCTGATCCAAGTTACAAGGTGATGCTTCCTCAGATTTCTGTTCACTCTTTTGGTTTTGGAT CAGATCATGATGCTTCAGTGATGCACTCAGTCTCTGAAGTCTCTGGTGGAACCTTTTCTTTCATTGAAAGTGAGTCTGTG ATCCAGGATGCTCTTGCACAGTGCATTGGTGGACTTTTAAGCGTTGCAGTGCAGGAGCTACGAGTAGAAATTGAAGGTGT GAGCCCAAATGTTCGACTTAGCTCGATAAAAGCTGGGAGTTACTCTAGCCTTGTAACTGGTGATGGCCACTCAGGGTTGG TTGATCTTGGTGATCTTTATGCTGATGAAGAGAGGGATTTCTTGGTTTCTATCAACATCCCTGTTGAGGAAGATGGACAT ACACCATTGCTAAAGCTGAGATGTCTCTACATTAATCCCTTGACAAAGGAGATTACAACGCTTGAAAGTCACGTGCTTCA GATTCGAAGACCAGAATATGTTGCTGAAGAAAAAGTTGTTCCCATCGAAGTGGTCAGGCAAAGAAACAGATTCCTAGCTG CAGAGGCAATGGCCCAAGCAAGAACTTTAGCAGAGCATGGAGATTTAGAGGCAGCCGTTAAAGCCATTGAGAATTTTAGA TTGGTTTTGGCAGAGACGGTTGCAGCAAAATCATGTGACAGGTTTTGTGTTGCTTTGGACTCGGAACTGAAGGAAATGCA AGGTAGAATGAGGAGTAGTCACATGTACGAGGCATCGGGAAGAGCTTATATTCTTTCTGGACTTAGCTCACACTCATGGC AAAGAGCAACAGCGAGGGGAAATTCAAGGGACAGTTCGAGCTTTGTCCAGGCTTATCAGACTCCATCAATGGCTGAGATG CTGCGTCGATCACAGGCCATGTTTCTAGTTAGTCCATCACATCATAGACTCATTCAGCCCTTGCTCTCTTTTGCTTCACA ACCAAAGCCAAGGTAAAGAACAAATTTTGACATCAATCCAATGCTTATGTACAAGTAGTTACGTAGCAAGCTTAAACTTG TTTTGTCTGTAAAGAAACAACCACAAGCAGTCGAA .....i.......................................................................... ................................................................................ ....................................................................i..........i .........i...................................................................... .............................i...............................N.................. .......................................................N............N........... .......N............................i...........................N........N...... ...........................N.................................................... ...............N..i............................................................. ...................N..N..N.............................N........................ ..............N.......N..i.....i................................................ .........N...................................................................... ...........................N...................................i................ ............................................................................i... ...N...............................................N....................N....... ..................N............................................................. ........................................i....................................... ......N..N..........i........................................................... .......N........................................................................ ........N.....................................................N................. ..................N.....N...............................................N....... ....................N........................................................... .................i.............................................................. ........i...........................N........................................... ................................i..........................................i.... .......N..............N....................................................i.... ....................................................................N........N.. ..................N............................................................. ........................................N.....N................................. ................................... Pos Score Pred ------------------------ 6 0.633 Yes 162 0.407 - 309 0.706 Yes 320 0.787 Yes 330 0.528 Yes 430 0.634 Yes 462 0.333 - 536 0.290 - 549 0.385 - 568 0.407 - 597 0.585 Yes 625 0.415 - 634 0.432 - 668 0.377 - 736 0.495 - 739 0.701 Yes 820 0.087 - 823 0.090 - 826 0.200 - 856 0.131 - 895 0.195 - 903 0.379 - 906 0.725 Yes 912 0.586 Yes 970 0.180 - 1068 0.387 - 1104 0.773 Yes 1197 0.805 Yes 1204 0.245 - 1252 0.408 - 1273 0.173 - 1299 0.487 - 1401 0.597 Yes 1447 0.302 - 1450 0.125 - 1461 0.815 Yes 1528 0.230 - 1609 0.177 - 1663 0.259 - 1699 0.125 - 1705 0.110 - 1753 0.178 - 1781 0.311 - 1858 0.549 Yes 1929 0.813 Yes 1957 0.099 - 2033 0.585 Yes 2076 0.747 Yes 2088 0.220 - 2103 0.423 - 2156 0.673 Yes 2229 0.443 - 2238 0.318 - 2259 0.264 - 2361 0.097 - 2367 0.064 -

Explain the output -- Processing locus 110: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGGGGTGGATTTGGGTTTGATTGATTTTTTCATATTACAAACAGCATTTACGCCTGAATTAATGATAAAGCTTTGGTCTT TTGAGAGTTTTCTTTAGATAACCTGAGTGTTGCACTAGGCGTTCAATGGAGACTGATGGCTTTCGCTTGATTTGTAAGAA CTGGTTTTATGTAGATGTTCTTTTTCCCTTTTTTTTAATTGTATGTTCTTGCGTTTTTAGTGTCATGTATTTCTCTTCTG GTAAGTATTCTGAATATCTTTTGCGATGCTTTCAGCCTCCACTTGGGGAAAAAGGGCGTCAAGGAGAAGAAAAGAGTTCT AATGGTGTTCTAACTCGTGAATCTGAAAGGGAGGATAAGAGGTGGGATAAAGAAGCTGGAGGTCCTTCAGAGAGAGTTAG TAAATTAAGTTATGATAATGGCAGAAGTTCGTTTTCTCCGTCAAACAGCAGGCAGTCTAACGAAGGCCGTGCCCGGAGTA GATCTAAGTCACATGATCGGGAGAGAGAAACTTCTAGGTCAAGGTTTGCGGAGGATGAGTTTTCGGACAGAGGAAGACAT CATGATTCTAGTAGGGATTATCATCATGACAGAGTTGACTCAGTTAGGACTGAGGAAAGATATCATCGACGTGGGCGATA TGAGGAAAATGACAGGCAATATAGTCGAGAAGTCTTGGAAAGAGATAGAAGTAAGGAGAGAGATATGGACAGGGAGGGAA GCATAAGAGATAGGGATTCGGAAGGAAGTAAGCGGAGAGATAAAGATAGTGATCGGAGGAGGGAAAGAGAACGAGAGAAG AGAAGGGAGATAGAGTCTGACCGTGAAAGACGAAAAGAGAAGGAACGAGAGCGCAGCATTGATAGGGATAGGAGAAAGGA GAGGGAAGGAGATTATTTACGGGATAGAGCCAATGAGAGAGGTAGGAGTAGAGATAGAACCAGGTATAACAGCAGAGAGA GGAAGAGAGAAAAGGAAAGGGAGGGTGAAAAAGATTGGGAGAGAGGAAGAGAAACCCAGAAAGATAGGGAGAAGTACATA AGTAGTGATGTGGACTACGGTGAGATGAGGCACAAACAGTCTAGAAACACAAGATATGATGCGGTAGATGACCTAGAAAT GAGAAAGCCAAATTCTTTGAAGGCCCATAATTCTAAGGGTGATAAGTTAGAAGAAACGTGGGCCAATGATGAAAGGTCCC GAAATGAGGATGGTCAAGATGATAATGACGAAGGACTTACATGGAAATCTCCTGAGGAAGAAGAAGAAGAATTACTAAAT AGAATCAAGGAAGAGAGCAGGAAGCGAATGGAAGCCATACTGGAGAAACATAAGAGGAAGCCCGAGCAGCAAAATGAACT TTTAACTCAGGATAATGGGAAAGATATTGTTCCAGAAACTGGTGCTCCAGTGTCGACTTCTCCTGCAGTTGTCATAGCAG CTAATGTAGGCCAGGCCAAAACTAACTTGGACTTTGATACTGTCGCTGCCAAGGCACCATTAATAGCCGGTGGGCCACCT ACTATGTCTGGAATTTCAGACTCAGAGAAGAATCAAGCTCAAGCGGGGCTTGGTGAAGGTAGCCCGAAGAGTGAAAGGTC AGCTGACATGTTTCATGATGATATCTTTGGAGAGTCCCCGGCTGGTATCCGAAAAGTGGGAGGGAAAGGTGATGGTGTTC CCATGGTGAGGAGCGGGCTTCATGATAATTGGGATGATGCAGAGGGTTATTATAGCTATCAGTTTGGCGAGTTACTTGAT GGTAGATATGAAGTCATTGCTACTCATGGAAAAGGTGTCTTCTCTACTGTGGTTCGTGCTAAAGACTTGAAAGCTGGACC AGCTGAACCTGAAGAGGTGGCAATAAAAATTATCCGTAACAATGAGACGATGCATAAAGCTGGAAAGATTGAGGTTCAAA TATTGAAGAAGCTGGCAGGCGCTGACCGAGAAGACAGGCGCCACTGTGTTCGATTTCTTTCAAGCTTTAAGTACCGTAAT CACCTTTGCTTGGTGTTTGAGTCGCTCCATTTGAATCTTCGTGAGGTCTTGAAGAAGTTTGGTCGCAACATTGGTCTCCA ACTCTCTGCTGTTAGGGCTTATTCAAAGCAGCTATTCATTGCTCTTAAACATCTGAAGAATTGTGGGGTACTTCACTGCG ATATAAAACCTGACAACATGCTGGTCAATGAGGGGAAAAATGTCTTAAAGCTTTGTGATTTTGGTAATGCAATGTTTGCA GGCAAAAACGAAGTTACACCGTATCTTGTTAGTCGCTTTTACAGATCCCCTGAAATTATTCTGGGGCTCACCTATGACCA TCCGTTGGATATATGGTCTGTTGGCTGCTGTCTGTATGAGCTTTATAGTGGAAAAGTTCTTTTCCCTGGCGCCACAAACA ATGATATGTTACGCCTTCATATGGAACTGAAAGGTCCCTTCCCTAAAAAGATGCTTCGCAAGGGAGCATTTATTGATCAG CACTTTGATCATGACTTGAACTTTTATGCTACAGAGGAAGACACCGTTAGTGGAAAGCTGATAAAAAGAATGATCGTAAA CGTGAAGCCGAAAGATTTTGGTTCCATCATTAAAGGTTACCCTGGTGAGGATCCCAAGATCTTAGCTCATTTCAGGGATC TCTTGGACAAAATGTTCATCCTTGATCCCGAGAGGAGACTGACTGTGTCACAGGCATTAGCTCACCCATTCATCACTGGC AAGTGAAACCACATTGCCATTTTCCCTCTCTGTTGTATCAGGAAATGCTGTTGTCCTAGATATCTGTACATTAAATATCT TAATTACCATTGAAGGATCTAATCT .............................................................N.................. .............................................N.........N........................ ........N.....N...........................N.....................N............... .........................N...................................................... ...........i.....i.............................................................. ............N.........................................N......................... ........N.......................................................i............... ................................................................................ ................................................................................ ................................i............................................... ................................................................................ .......N................i..............................N..N........N..........i. .................................................................N..N........... ...i.....N........N.....N...............i....................................... ...........................i.............................................N...... ..............N................................................................. ...N............................................................................ ...i............................................................................ .......i......N..N.....................................................N........ ..i..................N...........N..N.........................................N. .......N.................N...................................................... .........................................N.......i.............................. ................................................................................ ................................................................................ ................................................................................ .................N.........N...........N..........................N....N........ .........................................................................N...... ............N......................N............................................ N....N..............i.............................i............................. ..........N..............N...........................................i.......... ................................................................................ ...........N.................................................................... ............................................N................................... ......................... Pos Score Pred ------------------------ 62 0.332 - 126 0.178 - 136 0.177 - 169 0.203 - 175 0.144 - 203 0.143 - 225 0.444 - 266 0.396 - 322 0.307 - 412 0.534 Yes 418 0.614 Yes 493 0.273 - 535 0.329 - 562 0.330 - 586 0.393 - 640 0.354 - 649 0.406 - 705 0.752 Yes 913 0.532 Yes 1048 0.238 - 1065 0.795 Yes 1096 0.150 - 1099 0.224 - 1108 0.367 - 1119 0.708 Yes 1186 0.356 - 1189 0.239 - 1204 0.521 Yes 1210 0.280 - 1219 0.358 - 1225 0.399 - 1241 0.778 Yes 1308 0.731 Yes 1354 0.206 - 1375 0.137 - 1444 0.325 - 1524 0.676 Yes 1608 0.688 Yes 1615 0.182 - 1618 0.282 - 1672 0.345 - 1683 0.697 Yes 1702 0.065 - 1714 0.055 - 1717 0.201 - 1759 0.218 - 1768 0.189 - 1786 0.226 - 1882 0.494 - 1890 0.563 Yes 2178 0.387 - 2188 0.190 - 2200 0.209 - 2227 0.247 - 2232 0.437 - 2314 0.284 - 2333 0.343 - 2356 0.218 - 2401 0.241 - 2406 0.202 - 2421 0.753 Yes 2451 0.609 Yes 2491 0.189 - 2506 0.049 - 2550 0.753 Yes 2652 0.411 - 2765 0.117 -

Explain the output -- Processing locus 111: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATCATTTTTTCCTGCAATTCTCAGATTCTGTTTTTTTGTATGTCAGGAACAGAATTGAAACGTTTTGAAAGAAACAGG AGAATCTTTTGGTTCAAGTAATCTAAAACCATGGCAGCCAAAGATGGAGCTAAGAGCCAAGAAGACTATAAGCTTAAAGA CATGAAACCAGAGCTTGGAGAGAGATGGCCACATGGAGGACAGCGTGGAGGAACCGGATGGATTGGCAGTGAACGAGCTG CAAGCACATATGACCTTGTTGAGCAAATGTTTTATCTCTATGTTCGGGTCGTCAAAGCCAAGGATCTTCCTCCAAACCCT GTCACCAGCAACTGTGATCCTTATGTGGAAGTGAAAATAGGAAACTACAAAGGAAAGACTAAACACTTCGAGAAAAGAAC CAACCCGGAGTGGAATCAAGTTTTCGCATTTTCAAAAGACAAGGTCCAATCCTCTACTGTTGAAGTCTTTGTGCGCGATA AGGAGATGGTTACAAGAGATGAATACATCGGGAAGGTTGTGTTTGATATGCGTGAGGTTCCTACAAGAGTTCCTCCTGAT AGTCCACTTGCACCTCAGTGGTACAGATTAGAAGATCGGCGAGGTGAGAGTAAGAAAAGAGGAGAGGTTATGGTAGCGGT TTGGCTTGGGACGCAAGCCGATGAAGCATTTCCTGATGCTTGGCATTCGGATGCTTCCTCTGTTCAAGGAGAAGGTGTCC AAAGCGTGAGGTCTAAAGTCTATGTATCTCCCAAATTGTGGTATCTGCGGGTTAATGTCATCGAGGCGCAAGATGTTGAG CCTAGTGACAGGAGCCAACCACCTCAAGCTTTCGTTAAAGTACAAGTTGGGAATCAGATTCTGAAAACAAAGTTATGTCC TAACAAGACGACGAATCCGATGTGGAACGAAGATCTTGTTTTTGTAGCTGCAGAGCCATTTGAAGAACAGTTCTTCTTGA CGGTCGAAAACAAGGTGACACCAGCTAAAGATGAAGTCATGGGGAGGCTGATCTCACCTTTGAGCGTATTTGAGAAAAGG TTAGATCACAGAGCTGTTCATTCCAAATGGTACAACCTCGAAAAGTTTGGGTTTGGGGCTCTGGAAGGAGACAAGAGACA CGAGCTCAAGTTCTCCAGCCGAATCCACCTACGAGTTTGCCTTGAAGGAGGTTACCATGTAATGGACGAGTCAACTCTGT ACATCAGTGACGTGAAACCTACGGCAAGGCAACTATGGAAGTCTCCTATTGGCATCCTTGAAGTAGGAATCTTGAGTGCT CAAGGGCTATCGCCTATGAAAACAAAAGACGGTAAAGCGACAACAGATCCGTATTGTGTTGCCAAGTACGGTCAAAAATG GGTGAGAACAAGAACCATCATTGACAGCTCTAGCCCTAAATGGAATGAGCAATACACATGGGAAGTGTATGATCCTTGTA CAGTTATCACACTGGGAGTTTTCGATAACTGTCACCTTGGCGGAAGTGAGAAATCGAACAGTGGAGCCAAAGTGGATTCG AGAATCGGGAAGGTAAGGATCCGGTTGTCGACTCTAGAGGCTGATAGAATCTACACTCACTCCTACCCTCTTCTTGTTCT GCAAACAAAAGGGTTAAAGAAAATGGGAGAAGTCCAATTAGCTGTGAGATTCACTTGTCTATCCTTGGCTCACATGATCT ATCTTTATGGCCATCCTCTGCTTCCAAAGATGCATTATCTCCATCCATTCACTGTGAACCAATTAGACAGTCTAAGATAC CAAGCAATGAGCATTGTCGCAGCACGGTTATCTCGAGCAGAGCCTCCTTTAAGGAAAGAGAACGTAGAGTACATGCTTGA TGTTGATTCACACATGTGGAGCATGAGAAGGAGCAAAGCTAACTTCTTCAGGATAGTGTCTGTGTTTGCAGGCTTGATTG CTATGAGTAAATGGCTCGGAGACGTCTGCTACTGGAAGAATCCGTTGACGACGATATTATTCCACGTTCTCTTCTTTATA TTGATTTGTTATCCGGAGCTGATACTCCCAACCACATTCCTCTACATGTTCTTGATAGGGCTTTGGAATTTCCGGTTCAG GCCGCGTCATCCTGCTCATATGGACACAAAGGTATCTTGGGCTGAAGCGGCTAGCCCTGATGAGCTAGATGAGGAATTTG ATACTTTCCCAACTTCAAAGGGACAAGATGTTGTGAAGATGAGGTATGATAGGTTAAGAAGCGTTGCGGGTCGGATCCAG ATGGTTGTTGGAGACATTGCAACGCAAGGAGAGAGGTTTCAGGCTTTGTTGAGTTGGAGAGATCCGCGTGCGACTTGCTT GTTTGTGATTTTTTGTCTTGTTGCTGCGATGATTTTGTACGTGACGCCTTTCAAGATCATAGCTCTTGCGGGTGGGATGT TCTGGATGAGGCATCCTAAGTTCAGAAGCAAGATGCCTTCTGCTCCAAGTAACTTCTTCAGGAAATTACCTTCAAAAGCA GATTGTATGCTTTGAATTGTAAAACTTCTTTGTGGCTTTTAGGTCTTCTCTGCTCGTAACTTGTGTTTCTTGTGTTCGTG TGTTTATCTGCTCTGTTTTATTATTTTGTTCTGT .........................................i...................................... ..............................i............i.................................... .........N................i............N........................................ ......................N......................................................... ................................................................................ .....i............i............................i................................ .....................................................................i.......... ....................N..............N..............N............................. .....................N................................N.................N....... ..........................................................................i..... ...................i............................................................ ..............................i.......i......................................... ..........................N..................................................... ........................................................N....i.................. ..................................i............................................. ...............N.............................................................i.. .......................................i....i............N..........N........... ................................................................................ ................................................................................ ......................i..................................................N...... ......N......................i.................................................. ......i.................................................................N......N .............N........N......................................................... ..N.......i..................................................................... .............................................N.................................. ...................i.......................................N........N........... ...........................N..........N......N.................................. N............................................................................... ............................N...............................................N... .....N..........................N............................................... ......N......................................................................... .................................. Pos Score Pred ------------------------ 42 0.602 Yes 111 0.767 Yes 124 0.510 Yes 162 0.546 Yes 185 0.301 - 193 0.545 Yes 218 0.234 - 250 0.192 - 267 0.608 Yes 280 0.261 - 343 0.298 - 486 0.557 Yes 499 0.662 Yes 528 0.558 Yes 630 0.794 Yes 661 0.188 - 676 0.191 - 691 0.111 - 742 0.175 - 775 0.225 - 793 0.285 - 875 0.625 Yes 900 0.622 Yes 991 0.562 Yes 999 0.644 Yes 1067 0.373 - 1177 0.346 - 1182 0.730 Yes 1235 0.619 Yes 1296 0.234 - 1358 0.715 Yes 1400 0.574 Yes 1405 0.657 Yes 1418 0.499 - 1429 0.194 - 1623 0.744 Yes 1674 0.260 - 1687 0.171 - 1710 0.673 Yes 1767 0.661 Yes 1833 0.307 - 1840 0.222 - 1854 0.371 - 1863 0.486 - 1923 0.096 - 1931 0.649 Yes 2046 0.358 - 2100 0.591 Yes 2140 0.126 - 2149 0.075 - 2188 0.292 - 2199 0.483 - 2206 0.241 - 2241 0.167 - 2349 0.229 - 2397 0.289 - 2406 0.157 - 2433 0.415 - 2487 0.159 -

Explain the output -- Processing locus 112: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATTGGCCCACAAAAAGATCCCAGATGTGAATTAAAAAGCTGAGTCAAAACTCAAAAGCCACGTCAAATCAAACTCTGAT CCGTAGGGACCGGTTGGTTAGCGAATGTATAAATACCAACTCTCATTCTTTCACACCCTTTCGAGTCTCTCTACTCCTCT TCTTATTCACCGGAGTAACCCAAAAAAAAAAAGGAAAAAATATAAAAATAAATATTCTCCCCTAAAAATTTTGGAAAACC ACAAACAGAAATCTCTCTGTCTCACCCTAGAAAAACCTAGAAATCTTATCGCTATGGCTGCGGCGGTTGCATCTGGAATT GCCCCGACGACGGCCATGGTTGATCAAGTGATCCCTAATCAACCCACGGTTGCTGCTGCTGCTCCTCCTCCTTTCCCTGC CGTGAGTCAGGTTGCGGCGGTTGCTGCTGCTGCTGCTGCCGCGGAGGCTTTGCAAACCCATCCAAACTCGTCTCTCTATG TCGGAGATTTAGATCCGAGTGTCAATGAGTCACATCTCTTGGATCTCTTTAATCAAGTTGCTCCAGTTCACAATCTTAGG GTTTGTCGTGATCTGACTCATCGTTCTCTTGGTTACGCTTACGTCAATTTCGCTAACCCTGAAGATGCGAGTCGTGCGAT GGAAAGTCTCAACTATGCTCCGATCAGAGACAGGCCTATAAGGATCATGCTCTCTAATCGTGATCCGAGCACTAGATTGA GTGGGAAAGGCAATGTTTTCATCAAAAACCTTGATGCATCGATTGATAACAAAGCTTTGTATGAAACTTTCTCGTCTTTC GGGACGATTCTCTCTTGCAAGGTTGCAATGGATGTGGTGGGACGTTCGAAAGGATATGGCTTTGTCCAGTTTGAGAAAGA GGAAACCGCTCAGGCTGCTATTGACAAGCTTAATGGTATGTTGCTTAATGATAAGCAAGTCTTTGTTGGCCATTTTGTCC GTCGTCAAGACAGGGCACGTAGTGAGAGTGGTGCGGTGCCGAGCTTCACTAACGTTTATGTTAAGAATCTTCCTAAGGAG ATTACTGATGATGAGCTTAAGAAGACTTTTGGGAAATATGGAGATATTTCTAGTGCGGTTGTGATGAAGGATCAGAGTGG GAACTCGAGGTCTTTCGGGTTTGTCAACTTTGTGAGCCCTGAAGCTGCTGCAGTTGCTGTTGAAAAGATGAACGGTATCA GCCTTGGGGAGGATGTGTTGTATGTTGGTAGGGCACAGAAGAAGTCTGATAGGGAAGAGGAATTGAGGAGGAAGTTTGAG CAAGAGAGGATAAGCAGGTTTGAGAAATTGCAAGGATCGAATCTGTATTTGAAGAACCTTGATGATAGTGTGAATGATGA GAAGCTCAAGGAGATGTTCTCTGAGTATGGGAATGTGACTTCCTGCAAGGTTATGATGAATTCACAAGGTTTAAGCAGAG GTTTTGGTTTTGTTGCTTACTCCAATCCTGAAGAAGCTTTATTAGCTATGAAAGAAATGAATGGGAAGATGATAGGAAGG AAACCTCTTTACGTTGCTTTGGCACAACGTAAGGAAGAAAGACAGGCTCACCTTCAGAGTCTATTTACTCAAATCAGATC ACCTGGAACAATGTCACCAGTTCCATCACCGATGTCTGGGTTCCATCATCACCCACCAGGTGGGCCAATGTCAGGACCAC ACCATCCAATGTTCATAGGCCATAACGGTCAAGGTTTGGTACCTTCACAGCCTATGGGGTATGGGTACCAAGTTCAGTTC ATGCCTGGGATGCGTCCAGGTGCAGGCCCGCCTAACTTCATGATGCCTTTCCCTCTTCAAAGGCAAACTCAGCCTGGCCC TCGTGTTGGGTTCAGGCGTGGTGCTAATAACATGCAGCAGCAGTTTCAGCAACAACAAATGCTGCAGCAAAATGCAAGCA GATTCATGGGAGGCGCAGGCAACAGGAGGAATGGAATGGAAGCATCTGCTCCACAAGGCATCATCCCTTTGCCATTGAAT GCATCTGCAAACTCTCACAATGCTCCGCAACGCTCTCACAAGCCAACTCCCCTCACCATTTCTAAGCTCGCATCTGATTT GGCTTTGGCTAGCCCCGACAAACACCCACGGATGCTTGGGGATCATCTTTACCCACTTGTGGAGCAGCAGGAGCCAGCAA ATGCTGCTAAAGTTACCGGGATGTTGCTGGAGATGGATCAAGCTGAGATTTTGCACCTTCTTGAGTCACCTGAAGCTCTT AAGGCTAAAGTGTCCGAGGCTCTGGATGTTCTCAGACGCTCTGCTGATCCAGCAGCTGTGTCATCTGTTGATGACCAGTT TGCTCTATCCTCATCCGAGTGAACATACTGTTTACTCAAGACTATGTTTCTCTCGCTTTCTTAGACAGTTGCTTTAATAC CTTTTAGGACCTTTAAAACTAGCTTTTTCTTTTCTACATCT ........................i....................................................... ........................N....................................................... ................................................................................ .....................................................i.......................... ...............i................................................................ .............................................................................N.. ........................N....................................................... ................................................................N.............i. ..............N...............................i................................. ............N....................N..........................N................... ...........................i...N.......................N........................ ................................N....N.........N................................ .........................................................N...................... .......N..N..........................N.........................i................ ...................................................................i............ ............N........N.......................................................... .............................................................N...........N..N... .............i............N.....N...................N..N........................ ...............................................N........N...N.......i........... ................................................................................ ..........i....................i...................................i............ ........i............................................N......N................... i........i.............................N..N..................................... ...............................i..........................i............i........ .....i........................i....i..........................................N. ...................i............................................................ ...............................i................................................ N...................N...........i............................................... .........................N............................................N......... ...........................................N.................................... ......................................... Pos Score Pred ------------------------ 25 0.653 Yes 105 0.276 - 294 0.736 Yes 336 0.850 Yes 478 0.135 - 505 0.315 - 625 0.438 - 639 0.729 Yes 655 0.094 - 687 0.635 Yes 733 0.212 - 754 0.132 - 781 0.134 - 828 0.646 Yes 832 0.234 - 856 0.086 - 913 0.219 - 918 0.349 - 928 0.301 - 1018 0.069 - 1048 0.131 - 1051 0.110 - 1078 0.187 - 1104 0.767 Yes 1188 0.559 Yes 1213 0.109 - 1222 0.235 - 1342 0.232 - 1354 0.099 - 1357 0.140 - 1374 0.519 Yes 1387 0.184 - 1393 0.303 - 1413 0.496 - 1416 0.317 - 1488 0.401 - 1497 0.392 - 1501 0.298 - 1509 0.704 Yes 1611 0.530 Yes 1632 0.540 Yes 1668 0.503 Yes 1689 0.544 Yes 1734 0.406 - 1741 0.367 - 1761 0.501 Yes 1770 0.731 Yes 1800 0.116 - 1803 0.308 - 1872 0.562 Yes 1899 0.615 Yes 1912 0.708 Yes 1926 0.809 Yes 1951 0.583 Yes 1956 0.713 Yes 1999 0.269 - 2020 0.525 Yes 2112 0.739 Yes 2161 0.133 - 2181 0.430 - 2193 0.817 Yes 2266 0.179 - 2311 0.124 - 2364 0.067 -

Explain the output -- Processing locus 113: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATGTGATATCCTCCTTATGTAATGCTTCTTCACCCACAAATCTCACTTTATATATACTTATATGTTTGTCTTCTTCATC TATATATAAGCATTGCTCTAAACAGTTTATAGAAAGAAAAAAGCACCAACTTCTACATTCTTATTTCTCTCTTTTCATCT TCTTATTATAACCAAGGGTTTGGGTTCTGCAAAAATGGGTTTTATACGGCCACCGGCGGTGAGAAGCGGCGATTATATAG AAAGCATTTTCGGCGAATATTCCGCCGGGAAAACAAAACCTAGCCGGAAACTTAACTCGGCCAAGTTCGTGACGGCACTG ACGTGTCTTCAGTTCGCATTTGCCGTCTACGCCACCGGACTTCTCTACTACATGAGTCCTTCCATTGACTTAAGGGCCAA GCCAGACTTCACTTGGGCCACTAAATGGGCCCATAACATGCGTAGTTACATTGTTACCCCTCACGTCGTAAGCCATTACC AAGACTCTGCCTCTTTTCTCAAATCGGAGAATTTTCCGGTGATGGTTTCTCCAGCGGAAGTTTGCGAGTACGAGAAGATT GATTTCTCGCAGAAAAAATCAAACGACGAGAAAATGATCAAGATGAAGCGAGAGTTATACGACGACGTTTTGGGTTACCA GAGGAAGAATCTTGGGTCAGAGAGTTTAGAAGAGTTGATGAAGATGAAGTCAAAATGGGCTTTAAATGGGCCTAATAAGC CCAAAGTAACAGTAATACTAAATCACTTCAAGAGGAAAACACTTTGTGCTCAGCTTGATTCTCTTCTCCACCAGACGCTA CCGTTTCACCATGTCTGGGTTTTGGCCTTCGGGAGTCCTAACGAAGCTTCTCTCCGGCGAATCGCCGGAAGTTACAACGA TTCTCGAATCAGCTTCATCAGCTCAAACTACGATTTCAAATACTATGGAAGATTCCAGATCGCGCTTCAAACAGAAGCAG ATCTGGTTTATATCCTCGACGATGATATGATTCCTGGGAAGAAAATGCTTCAGATGCTCTCACACGTCGCCGGAACTGAG AAATACGAAAACTCTGTTTTGGGAAGTATCGGAAGGATTTTACCTTTCCGGCAAAAGGATTTCACGTTTCCGAGCTATAG GAAGTTTAGATCTAAGGAAGCTGGCCTTTACTTGCCTGATCCAGCTTATGACATCACCTTAGATCGGATTCTTCAGGTTG ATTTCTTATCAAGCTCGTGGTTCTTATCGGCGGAGCTCGTGAAAGCGTTGTTCATCGAAAAACCATTCACATTCTCTACC GGTGAAGATCTTCATCTCAGTTATCAGCTTCAGAAATACAGAAATGCAGGTTCGTTTGTTCTTCCCGTTGATCCAAATGA TAAGGAGACTTGGGGAGATAGTGAACACAGGTTAGCTTATGTGTCTGAGACTACTGTGATATTTAAAAACATTGTTGAAG TAAGAGATAACCAGTGGTGGAAAGCGCTTTCTACGGGTTATGTGACTCAATGGGCTGCAATGTATCCTCAGAAGATTGAT GCTTTGTTTTACGCGCATTCGATTGATGAAGTTAAAGCACTTGGTCCTTTGCTGGAGAAGTTTAGAGGTACTGTTGGTAA AAAGGCTTATATCGCGGTTTCGGGTGGGAAGTTCTGTCCGTGTGAGGATGCGGCGTCGGCTTTGAGGTGGCCTAAGGTTG TTTGTAAGGAGAGGAGATTCAAGATTTTTGATTTGGAAGTTGGGGCTATTTTGGGTGTGTCGAACTCAGAGGTGCCTGTA TTTCAAGCTGTGTATTCGAGCATGAAAGGGCTTATTAAGATTCATAATCCAAGCGTGGTGATTACGGTAGCTGATGCTGA TCCGAATGTCAAGAAAGCTTTGAAAATGGCTACCGAGACTAATTCTAATGGCACCGCATTGGTTCTTCTACCGAGAGCTT CTATCTCCAAGGTTCTCTGGATGGCTGATCTAAGATCAACAGCATTGCCAAACTGGAATAAGATGAGAGTCTCAGTGAAC ATCATCACCCAAAACCGAGCTCAATCTTTATTAAGATTGCTTAGGTCTTTGAGCAATGCATACTACCTTGGTGACGAAAT ATCACTCAGCTTCAACATGGATAGCAAAGTGGATGAAGAGACGATCAATGTAGTGAGCACATTCGATTGGCCTCATGGAC CAAAAACACTAAGAAGAAGAATCATCCAAGGAGGCTTAATCCGTGCTGTGAGCGAGAGTTGGTATCCAGCTTCTGATGAT GATTTTGGTCTCTTGCTTGAAGACGACATTGAAGTCTCTCCATATTATTTCCTCTGGATCAAATACGCTTTACTTGCTTA CCACTACGACCCTCAAGTCTCTTTCCCTGAACTCTCCTCGATCTCTCTTTACACGCCCAAAATCGTTGAAGTTGTCAAAG AGAGACCCAAATGGAACCCAACAGATTTCTTCAAGCAAATCCATCCTCACACACCTTACCTTCACCAATTACCATGCAGC TGGGGAGCCGTTTTCTTCCCAAAGCAATGGAGAGAGTTCTATGTCTACATGAACATGAGATTCACAGAGAACGCGAAAGC TAACCCGGTTCAGATTCCGAAATCAAGAACCAACGGTTGGCAAGCGTCATGGAAGAAATTCTTAATCGACATGATGTACC TGAGAGGATACGTTAGTCTATACCCAAACTTCCCTAACCAATCAAGCTTCTCAACCAACCACATGGAACCAGGAGCTCAC ATCGCAGCTAAAGACAATGTGGTGAAACACAACAAAACCGATTTCGAAGTTCCATTGCTTATGGATGATTTCAGAAACTT CTTACCGAACCAGAAACTCCCTCCGCTTTCTAAGCTTCCATCACTCAATCTCTTCAACATGCCGGTTTCGCTTAAAGGTC TTAAAGCTGCAGGAGCTAAGCTTGGTCAAGATGTTCTTCGTTGCAACAATGTCTCTGAGATTGTCGCCGTCAATCATCAG ACAGGTTTACCGGCTAGATGCATGAAATTCTGAAGGTAGTTTTATCTAACGGCCAAATAAATAAAAAAGTTATTTGGCTG TGATTTTTCTTTCTTACATCTGTGGATTCTTGTTGATTCTTTGTTTTTTGAA ................................................................................ ..................................i............................................. ................................................................................ ...................................................N............................ ........................N............i.......................................... .........................................i...................................... .................................N........N..................................... .....................................N.....N..........i..........N.............. ................................................................................ ..........i..................................................................... ............................................N................................... .....................N....N.................N........N.......................... ................................................................................ ...............................................N................................ ................................................................................ ...........................................N................................N... ......................................N......................................... .......................................N.........N.........i..................N. .........................N...................................................... ...............................................N................................ ................................................................................ .....................i...................................................N...... .....N...................i.....................N................................ ....................N.........................................N................. .......................................................N........................ ................i...............N..............i..........................N..... ...........................................................................N..N. ................................................................................ ................................................................................ ..........i..............................................................i...... ..........................i.............i.......N.....N......................... ................................................N.....................i..N...... ..............................................................i................. ................N...........................................N...N............... ..........................................................i..................... ..............................N.................N............................... .................N...N.......................................................... .................................................... Pos Score Pred ------------------------ 2 0.376 - 18 0.277 - 23 0.345 - 63 0.147 - 195 0.666 Yes 372 0.434 - 425 0.205 - 438 0.826 Yes 522 0.642 Yes 594 0.337 - 603 0.349 - 678 0.263 - 684 0.174 - 695 0.672 Yes 706 0.220 - 811 0.589 Yes 925 0.296 - 982 0.222 - 987 0.478 - 1005 0.353 - 1014 0.297 - 1168 0.124 - 1324 0.347 - 1357 0.095 - 1399 0.128 - 1480 0.078 - 1490 0.361 - 1500 0.765 Yes 1519 0.070 - 1546 0.218 - 1648 0.096 - 1782 0.786 Yes 1834 0.078 - 1846 0.102 - 1866 0.790 Yes 1888 0.126 - 1941 0.277 - 1983 0.470 - 2056 0.416 - 2097 0.668 Yes 2113 0.380 - 2128 0.512 Yes 2155 0.166 - 2236 0.156 - 2239 0.299 - 2411 0.688 Yes 2474 0.750 Yes 2507 0.721 Yes 2521 0.532 Yes 2529 0.233 - 2535 0.268 - 2609 0.489 - 2631 0.552 Yes 2634 0.476 - 2703 0.675 Yes 2737 0.432 - 2781 0.306 - 2785 0.272 - 2859 0.824 Yes 2911 0.375 - 2929 0.236 - 2978 0.195 - 2982 0.197 -

Explain the output -- Processing locus 114: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTGAATGGTTCTGCTTAGTGTGTAGTCTAACTGTTTGATTAAATGTCGCAAGATGAAGTTTTTCTTGTGCTGTTTCGT CTGTCAATGAAGCTTTTCTGAATTGAATTTTCATGGGTTTTGATGTTGCAGATGGCTACATATAAGCCTAAGAACATCCT CATCACTGGGGCTGCTGGATTCATAGCCTCTCATGTTGCTAACAGACTAGTTCGCAGCTACCCTGACTACAAAATTGTTG TGCTTGACAAGCTTGATTACTGTTCTAATCTGAAGAACCTTAATCCTTCTAAATCCTCTCCCAACTTCAAGTTTGTGAAA GGAGATATCGCCAGTGCTGATCTCGTCAACTACCTTCTCATCACTGAAGAAATCGACACCATTATGCACTTTGCTGCTCA AACCCATGTTGACAATTCTTTCGGTAATAGCTTTGAGTTTACCAAGAACAATATTTATGGTACCCATGTCCTTTTGGAAG CTTGTAAAGTCACTGGCCAGATCAGGAGGTTCATCCATGTGAGTACTGATGAGGTCTATGGAGAGACTGATGAGGATGCT TCAGTGGGTAATCACGAGGCTTCTCAGTTGCTCCCAACTAATCCATACTCCGCCACTAAAGCTGGAGCTGAGATGCTTGT CATGGCATATGGTAGATCATATGGGTTGCCGGTTATAACAACTCGCGGGAACAATGTTTATGGTCCTAACCAGTTTCCTG AAAAGTTGATTCCTAAGTTCATCCTCTTGGCCATGAATGGGAAGCCTCTCCCAATCCACGGAGATGGATCTAATGTGAGA AGTTATCTCTACTGCGAAGATGTTGCTGAGGCATTTGAGGTTGTTCTTCACAAAGGGGAAGTTAACCATGTCTACAATAT AGGGACAACGAGAGAAAGGAGAGTGATTGATGTGGCTAATGACATCAGTAAACTCTTTGGGATAGACCCTGACTCCACCA TTCAGTATGTGGAAAACCGGCCATTCAATGACCAGAGGTACTTCCTCGATGACCAGAAGCTGAAGAAATTAGGATGGTGT GAGCGAACCAATTGGGAAGAAGGACTGAGGAAGACAATGGAATGGTATACTGAGAACCCTGAGTGGTGGGGCGATGTTTC TGGAGCTCTGCTTCCTCATCCACGGATGTTGATGATGCCCGGTGACCGACACTCTGATGGCTCTGACGAGCACAAGAATG CAGATGGTAATCAGACATTCACGGTGGTTACTCCCACCAAGGCTGGTTGTTCCGGAGACAAAAGATCCTTGAAGTTCCTC ATCTATGGGAAGACTGGGTGGCTCGGTGGTCTTCTGGGAAAACTATGTGAGAAACAAGGGATTCCTTACGAGTATGGAAA AGGAAGACTAGAGGATAGAGCTTCTCTCATCGCAGATATTCGCAGCATCAAACCAAGTCATGTCTTCAACGCCGCTGGTT TAACTGGGAGACCCAATGTTGACTGGTGTGAATCTCACAAAACTGAAACCATCCGAGTCAACGTTGCTGGAACTTTGACT CTTGCAGATGTTTGCAGAGAGAATGATCTGTTGATGATGAACTTTGCCACTGGTTGTATATTCGAGTATGACGCTGCACA TCCAGAAGGTTCAGGGATTGGTTTTAAGGAAGAAGATAAACCGAATTTCACTGGTTCTTTCTACTCAAAAACAAAGGCAA TGGTGGAAGAGCTTCTAAGAGAATTTGACAACGTATGCACCTTGAGAGTGCGGATGCCAATCTCATCTGACTTAAATAAC CCGCGAAACTTCATCACGAAGATCTCGCGTTACAACAAAGTGGTGAACATTCCAAACAGCATGACCATACTAGATGAACT CTTACCAATCTCGATCGAGATGGCGAAGAGGAACCTAAGGGGAATATGGAACTTCACCAATCCAGGAGTGGTGAGCCACA ACGAGATATTAGAGATGTACAAGAGTTACATCGAGCCTGATTTCAAATGGTCCAACTTCAATTTGGAAGAACAGGCTAAG GTCATTGTTGCTCCACGGAGCAACAACGAGATGGATGGTGCCAAGCTCAGCAAGGAGTTTCCAGAGATGCTTTCCATCAA AGATTCGTTGATCAAATACGTCTTCGAACCTAACAAGAGAACGTAAAACACAGACACACACACGCACTTCCCCGCTCTGT GTTCACTATAATGTTTCTACTATAGTTTTATCTGATTCATTTACTCTCTTTTTTTGCATTCTACG ......N.....................................N.........N......................... ......N.........................N.........N........i............................ ................................N............................................... ................................................................................ ...............................................................i................ .....N..................................................N........N.............. ....................................N...........N........N...........N.....N.... ........................................................................i....... ................................N...N..........................N........N....... ...................N...............................................N............ .............................N........N......................................... ......N....................N....................N........................i...... ....................................i....N...............................N...... .........................i.....i..i.....................N....................N.. ...N............................................................................ ....i.......................................N............................N...... ...........................................................N.................... ...............N................................................................ .......N..............i..........N..i..............................N............ ...............................................................................i ..................................i..................N.......................... ............................................................N............N...... ...................N.........................N.................................. ..............i...............................N................................. ..............................i...N...............................N............. ................................................................................ ..........N...................................................... Pos Score Pred ------------------------ 7 0.168 - 45 0.477 - 55 0.195 - 87 0.231 - 113 0.346 - 123 0.497 - 132 0.786 Yes 193 0.136 - 384 0.503 Yes 406 0.303 - 457 0.214 - 466 0.195 - 517 0.189 - 529 0.326 - 538 0.188 - 550 0.224 - 556 0.193 - 633 0.516 Yes 642 0.643 Yes 649 0.241 - 661 0.255 - 694 0.384 - 700 0.267 - 753 0.330 - 757 0.294 - 784 0.439 - 793 0.440 - 820 0.172 - 868 0.255 - 910 0.378 - 919 0.381 - 967 0.317 - 988 0.414 - 1009 0.233 - 1034 0.575 Yes 1077 0.525 Yes 1082 0.179 - 1114 0.280 - 1146 0.628 Yes 1152 0.646 Yes 1155 0.760 Yes 1177 0.484 - 1198 0.446 - 1204 0.348 - 1285 0.526 Yes 1325 0.365 - 1354 0.253 - 1420 0.311 - 1456 0.191 - 1528 0.282 - 1543 0.536 Yes 1554 0.450 - 1557 0.590 Yes 1588 0.493 - 1680 0.633 Yes 1715 0.534 Yes 1734 0.432 - 1821 0.463 - 1834 0.373 - 1860 0.388 - 1886 0.386 - 1935 0.603 Yes 1967 0.411 - 2031 0.757 Yes 2035 0.419 - 2067 0.450 - 2171 0.047 -

Explain the output -- Processing locus 115: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAAACTTGTAGGTTAGAAAGGAGATGAAATGAGAAAGTTAGGGGCTTTTCTACAATTGTCTTCGTTTATGATCACGAA TTGAATAGTTGACAAGTCTAAAATTTGTCTCTTTCCTTGTCACTTGTCAGTGAGTATACAAAACAATACACAGAGCCTCG CATGTCATATTCACACAGGGACCTTCCGTTAAACTCCTTTTTTATAATCTCGTTCTCTCTTCTCTCTTTCTCTCTCTGGT TGCTCTTGGCCATGGTTGTGAATAGTCAAGCTTTCTTGTTGGCATTAATTGCATTACTTGCTACCCAATTGCCTTCACTA ATGGCTGCAGATTGTCCATTAGATTTCAGTGGATCAAATTTCACTCTAGTAGCTACTGTGTGCTCCAACATAACCAACAG AGGCAAATGTTGCCGCTACATGAACGCTTTTGTTGCGGTGTCCGTGGCTCGTTACGCGAACTTGTCAACTAATCTTGGAG TCACATCAGATTTATCTGAAACCTGCATTGCTTCCATCTCTAGAGCCATGGAGGGCTACGGAGTCTCGAGAAACGCCACT AGTTTCTGCGGTTTGGGAACCAAGATCCTTGTTAAGTATGACTGTGACGGTCGAACCACTGTTACACAAATGCATCAGTC TCCGGGATTTGGACATGTTTCAAGAAACTGCAGACTCCCATTTTCGCCGGGACACCAATGCAGAAAGTGTTTGAACTCTG GCATCACTTACCTTCGTAATCTCATAGGTGCAGAGACTAATAACATTACTCTGTGTACTTGTCGTGATGCGACTTATGCT ACATTAGCAAGCCGAATAGACGATACATCAGCTCTTGAACTCCTTAGTTGTTTCTTTCAAGTGACAGAGCTTAACATTCC TTCAGAGTCATTTTCACCAGTGGCAAGTCCAGAACCTTCTCCAAGCACGGTTGGTGGTATTAGCCCAAGCAACAGTGATT CTCAAATGACTACAAGTAGAAGCACCAATCCATATCACTTAACAATGGTTCCAACCATTGGGATTGTTGTTACAGCTGTT GCTCTTACGATGCTTGTAGTCTTGGTAATTCTTATCCGTAGAAAGAACCGGGAACTGGATGAATCTGAGAGCTTGGATAG AAAGTCAACAAAATCAGTTCCTTCTTCCCTTCCTGTATTCAAGATTCATGAAGATGATTCTTCCTCAGCTTTCAGAAAGT TCAGCTACAAGGAAATGACAAATGCAACAAATGATTTCAACACGGTTATCGGTCAGGGAGGTTTTGGTACTGTTTACAAA GCCGAGTTCAACGATGGTCTGATTGCAGCGGTAAAAAAGATGAACAAAGTCTCTGAACAAGCTGAACAAGATTTTTGTAG AGAGATAGGGCTTTTAGCTAAGCTGCATCACCGTAACCTCGTTGCTTTGAAAGGCTTTTGCATCAACAAGAAAGAAAGGT TCTTGGTCTACGACTATATGAAAAATGGCAGCTTAAAAGATCACTTACATGCCATTGGAAAGCCTCCACCTAGTTGGGGA ACAAGAATGAAAATTGCAATTGATGTGGCAAATGCTTTGGAATATCTTCATTTCTACTGTGATCCTCCTCTCTGTCATAG AGATATTAAATCAAGCAATATATTACTCGATGAGAACTTCGTCGCTAAGCTTTCAGATTTTGGCCTTGCACATTCGTCTA GAGATGGCTCTGTTTGCTTTGAGCCTGTGAATACCGATATCCGTGGAACTCCAGGTTATGTAGATCCGGAATATGTAGTA ACGCAAGAGTTGACAGAGAAGAGTGATGTGTACAGCTACGGAGTTGTGTTGCTGGAGCTCATAACCGGGAGAAGAGCAGT TGATGAAGGTAGGAACCTTGTGGAAATGTCTCAGCGGTTTTTGTTGGCGAAATCAAAACACCTCGAGTTGGTAGACCCGA GAATCAAAGACTCTATTAATGATGCTGGAGGAAAACAACTTGATGCAGTTGTGACGGTTGTGAGATTGTGTACCGAGAAA GAAGGCCGGTCTAGGCCATCAATTAAGCAAGTCTTGAGGCTGTTGTGTGAAAGCTGTGATCCGGTGCATAGCGCATTTGC TAAAGCGGTTGAAGAAGAGATAGGCTGGGATAGTAGGAAGAGAAGCAATTTGAGAATTCAAAGAGGTGATTCCCGGATCT TTGGTCCATCGTCTAGTACTACTTCCAGGTCACATTACAGCCGGAGTTTACCTCACTCTCCAATTAACGGATTTTCTTTC TGAGTTAGTCACATCTTCTCTATAGCTAAATCTGAAAGTTTTACATATGTTTATGAAAATTATGAATTCATATTGAAAGC TCTCATACTCTACACAGACTGC .........................N....N......................................N.......... ................................................................................ ...........i.................................................................... i............................................................................... ......i............i............................................................ ...............................................i................................ .....................................N...............................N.......... ..............i..........................................N...................... ..................................................................N........N.... ................................................................................ ................................................................................ .....i......................................i................................... .........N................................................N..................... ...............................................N.....N.......................... ..............i......N........N................................................. .............N.........................N........................................ ................................................................................ .................N......N.......................N............................... ......i...............N........N................................................ .............................N.................................................. ...N.....................................................N..............N....... .........................N...................................................... ..N......................i...................................................... ..................i..N....................N..................................... ................................................................................ ................................................................................ ................................................................................ ..............................................N.....N........N.................. ...................... Pos Score Pred ------------------------ 26 0.116 - 31 0.442 - 70 0.179 - 162 0.217 - 252 0.707 Yes 321 0.644 Yes 407 0.510 Yes 420 0.533 Yes 528 0.831 Yes 598 0.488 - 630 0.249 - 655 0.663 Yes 698 0.128 - 787 0.074 - 796 0.115 - 966 0.787 Yes 1005 0.863 Yes 1050 0.308 - 1099 0.141 - 1168 0.309 - 1174 0.376 - 1215 0.889 Yes 1222 0.263 - 1231 0.357 - 1294 0.249 - 1320 0.409 - 1458 0.370 - 1465 0.473 - 1489 0.185 - 1527 0.582 Yes 1543 0.215 - 1552 0.306 - 1630 0.354 - 1684 0.402 - 1738 0.252 - 1753 0.173 - 1786 0.175 - 1843 0.179 - 1866 0.616 Yes 1939 0.527 Yes 1942 0.420 - 1963 0.349 - 2287 0.142 - 2293 0.053 - 2302 0.051 -

Explain the output -- Processing locus 116: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTACAAACAAGTTCATTCAGATGTAGAGAAGGATCGATAACAAACTTTAAAAATTGGATATAGTCAGGCCTTGGAAGTA TCGCCTTGGGTCAGACTTTAGTTGTTTCACATGGCGTGGACGCTGGATCTCAGTGGCAACAATAATCAATATTTCACGTG GTATGGCATTAATTTTGATCATATGCATTATTTGATTATTCATCGTTATATGCCCACTTTGAGAGATCCGATAGAAACAA AAATAGAAAAGAAAAACATCACAAAAGAGATCTGTGGTTTGATTTGGATCACCATGGAAAAAGTAGAGGTACCACCAATT CATAACCATCCGCTGTTACCTTTTACTCGTTTTTCCTACAAGGCTTGTAAAGGGTGTGGGTATGTTGGTTACATATATGG AGGCTACCATTGCAATGAGTTGGGGTGTGAATCTGTGTTTCATAAAGAGTGTGCCGACCCGTTACCAGAAATCAACCACT ATTCTCATCCAGACCATCCTCTAAAGCTCCTCATAAATGTCGAACCACTTGCTTCTTGTAGCCAGTGTCCATCTTCGTTT GAAAATGGTTATCTTTGTTCGATTTGTGACTTCAAGTTGGATTTTCGTTGCGCGACGAGGTTGCCTTTGGAATTTATTCT TGAAGAATCCACTGTCCACGAGCATCCGATTCAGCTCTCTGATGGAACCCGTGACGGTTTTGTGCAACTTTGCAAAACAT GCAAATGGGGAGTTTTCGATGCTGTTCATTACAAATGTAATAAATGCGATTTACGCTTTCATGTGGAGTGTACCAAACTT TTTCCACAAGCTAGCCACGTTTTACACCCCCAACACCCACTCAAGCTCCTTAGATGTGAAGAAGCACCCGATTACGCCGA CAACAAGTGTCTTCTTTGCGGGAATAAGTTTGACGGACGAATTCACCACTGTGATGTTTGTAACTTTACCATATGTGGCG GCTGTATGGAAAACCCACCGCCGCTTAGTGTTGTAAGCCCGACCACCCATGAGCATCAACTTTATCTTCTTTCAAAACCT CTCGGTTTCACTTGCAATGCTTGCGGGACTCAAGCTGATCAAAGCCCTTATTTCTGTCTTCAATGCAACTTCATGATTCA TTGGGAGTGTATCGATTTGCCCCGCGTCATAAACATCAACCGCCACGATCATCGCATCTCCTACACTCGTCGTCTTGGAC ATGGGCAAAATACATGCGGGGTTTGTCGTAAAAAGGTTGATGGCTTCTACGGGGGTTATTCTTGCTCAAAATGTTCTAAC TATGTTGTTCACTCGAGATGCGCAACGGACGAGGATGTATGGGACATGATAGAGCTAGAAGGGACACCCGAAGAGCCAGA GGAAGATGCGCCATTCGAGGTGATTGATGATAACACGATCAAACATTTTAGCCATGATCATAACTTAGTAATCAACAAGG ATGGGAGGATTCTCCATGAAAGCACACTATGTAAAGCATGTGTCTTCCAGATCTGTTCAGATGCGTTCTACAGTTGCGAG AATTGTGACTTCATTCTCCACCAAAAATGTGCTAACCTGCCTCGGAAGAAACGGCATGTTTGCGACAACCTACCATTTAC CCTAGTCACAAATGATACTTCAGACGAAACTTCATGTGATCTTTGTTGGCAAAGTTTCACCGGTTTCAGGTACGAGTGTC TTACTCGTATGGTTACTCTAGATGTACGATGTGGTTCAATTCGAGAACCATTGGTGCACCAAAGCCATCAACATCCCTTA TACTTTTCGAAACGTAGTTCTAAGTCTTGTACGGAATGTGGCAAAAACATGGATCTAGATATAGGAAGCTTTAGCTGTGA TGAGTGTGACTATAGTTTGGACTTCAAGTGTGCTCTTTTACCGCAGAAAGTAAAGTGGCATAGATACGACGATCATCCTC TTTACTTGTCGTGTGGTGAAAGTAGTGTGGATGGAGAATACTGGTGTGAAGCATGTGAAACAAAAGTGAATTCGAAGAAA TGGTTCTATACATGCAATGATTGTGGTGTCATATTGCATATTTCATGCGTTGTGGGAGACTTCTCATATTATATGCCAGG TTCCAAAATAGGATGGGAGGAAGACTTGGTGGTATCCAACACCTCTATGTGTAGACCATTTTGCTATGTGTGTAACTCTC AATGCAAGCTTCCTTCCATTGTTAAGGTCGTTTCCAAAGCCGATGTTGTTTTATACATTTGTTCTTTAAACTGTTATTAT AATTTCAACCGCTCTAATAACTACTCTAGTAGTGATTGGTAAGGCATGTTTTAATGAAGAAAAACTTTGTACCATTTAAT TCCAAAAGTATGTTTTACAATAGTTTTTTTTTAACCTCAAGCTTATGCGCTTTTTCCACAA .....................N.......................................................... ..............................N................................................. ..N...................N..........................N.............................. .....................................................N.......................... .............................................................N..............N... ..............N................................................................. ....................................i........................................... ....i........................................................................... .........................................N....................................N. ....N.............N...............N........N................i................... .....................................................N.......................... .....................................................N..................N....... .....i..........................................N............................... ................i.............................................N.........i....... ................................................................................ N............i.........................i..............................N......... .....N....................N..........................N.......................... i..............N............i........N......................N................... ..........................N............................i........................ ...........N.....................N.............................................. ........N............N......N................................................... ...................................N............i..............................N ................................................................................ ..............................N.....................N..........................N ...........N....N...........................N...........................N....... ............N.................................N..................N.............. .............................................N.......N.......................... .........N..................................N................ Pos Score Pred ------------------------ 22 0.294 - 111 0.477 - 163 0.282 - 183 0.163 - 210 0.429 - 294 0.216 - 382 0.433 - 397 0.496 - 415 0.464 - 517 0.714 Yes 565 0.531 Yes 682 0.375 - 719 0.171 - 725 0.321 - 739 0.357 - 755 0.444 - 764 0.430 - 781 0.550 Yes 854 0.145 - 934 0.353 - 953 0.170 - 966 0.521 Yes 1009 0.302 - 1057 0.545 Yes 1103 0.127 - 1113 0.727 Yes 1201 0.494 - 1214 0.537 Yes 1240 0.529 Yes 1271 0.500 - 1282 0.318 - 1298 0.229 - 1315 0.200 - 1319 0.648 Yes 1326 0.632 Yes 1366 0.141 - 1387 0.171 - 1414 0.261 - 1441 0.850 Yes 1456 0.411 - 1469 0.571 Yes 1478 0.236 - 1501 0.374 - 1547 0.148 - 1576 0.500 - 1612 0.271 - 1634 0.137 - 1689 0.296 - 1702 0.327 - 1709 0.434 - 1796 0.241 - 1809 0.776 Yes 1840 0.360 - 1951 0.355 - 1973 0.161 - 2000 0.376 - 2012 0.136 - 2017 0.400 - 2045 0.221 - 2073 0.314 - 2093 0.164 - 2127 0.267 - 2146 0.258 - 2162 0.066 - 2203 0.442 - 2286 0.221 - 2294 0.174 - 2330 0.046 - 2365 0.042 -

Explain the output -- Processing locus 117: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGTTTGATAAGTTATGTTCCACGAAATTATGTGAAAATTGATTTGTTTTCTTCTTTAGTTTGGTGCTTTCACACTGTTA ATCCACAAAGCCTTGTTGTAAATCTTGACCAATGGCCTAAAAGTCAAACCTTTGACTTTTCAGTGTTTCTTCTCAACACT CAAGAGATGGATGATAATATTCACAGATGATCAAAATCTTTCACACAAACCTAAAAAACACCCACCAATGTCTCGTGAAC TTATTATTCTCTGCCAACCAATTCTTGTTCTGTTCCTTACTCTGTTTTACAATTCTCATGGCTACTTCGTCTCACAAGGA TCTGTAGGAATCGGCTTCAACGGTTACTTCACTTTAACCAACACCACAAAACACACATTCGGTCAAGCTTTCGAAAACGA GCACGTTGAAATCAAGAACTCATCGACAGGTGTCATCTCATCTTTCTCAGTCAACTTCTTCTTTGCGATCGTCCCTGAAC ATAACCAGCAAGGCTCACACGGTATGACTTTCGTCATCTCTCCCACAAGAGGCCTTCCCGGAGCTTCCTCCGATCAATAC CTCGGAATCTTTAACAAAACAAACAACGGTAAAGCCTCGAATAACGTTATAGCTATCGAGTTAGATATTCATAAAGACGA AGAGTTTGGAGATATTGATGATAATCATGTTGGGATTAATATTAACGGTTTGAGATCTGTTGCCTCTGCTTCTGCTGGTT ACTATGATGATAAAGATGGAAGCTTTAAAAAACTTTCTTTGATCAGCAGAGAGGTAATGAGGCTTTCCATCGTTTATAGT CAACCTGATCAACAGCTCAATGTTACTTTATTCCCTGCTGAGATCCCTGTTCCGCCACTAAAACCGCTCTTGTCTTTGAA CCGAGATCTCTCGCCGTATTTGCTTGAGAAAATGTATCTTGGATTCACTGCATCGACGGGGTCAGTTGGAGCAATTCATT ACTTGATGGGTTGGTTAGTTAATGGTGTAATCGAGTATCCGAGATTGGAGCTTAGTATACCAGTCCTTCCTCCATATCCA AAGAAAACATCTAATAGAACGAAAACTGTTTTAGCCGTCTGCTTAACGGTATCTGTGTTTGCTGCGTTTGTCGCTTCGTG GATCGGTTTCGTCTTCTATTTGAGGCATAAGAAGGTTAAAGAGGTTCTTGAAGAATGGGAGATTCAATATGGACCTCATA GGTTTGCTTATAAGGAGCTTTTCAATGCCACAAAGGGTTTCAAGGAGAAACAACTTCTTGGTAAAGGAGGCTTTGGTCAA GTCTATAAAGGAACACTTCCGGGTTCTGATGCAGAGATCGCTGTGAAGCGGACTTCTCATGATTCAAGACAAGGGATGAG CGAGTTTCTAGCCGAGATATCGACCATTGGTCGTCTCAGACATCCAAATTTAGTCAGGCTTTTAGGATACTGTAGGCATA AAGAGAATCTCTACTTGGTGTATGACTATATGCCTAATGGAAGCCTTGACAAGTATCTAAACCGTAGCGAGAATCAAGAA CGGCTTACTTGGGAACAACGTTTCAGGATCATCAAAGATGTTGCAACTGCTCTACTACACCTGCATCAAGAATGGGTACA AGTCATCATTCATCGAGATATCAAACCAGCTAATGTTTTAATCGACAATGAAATGAATGCGAGGCTCGGGGATTTCGGAC TGGCGAAACTGTATGATCAGGGATTCGATCCTGAAACATCTAAAGTAGCGGGAACATTCGGATATATCGCACCAGAGTTT CTAAGAACAGGAAGAGCAACCACAAGCACTGATGTTTACGCCTTTGGGTTGGTAATGCTTGAAGTAGTGTGTGGTAGAAG GATAATTGAGAGACGTGCAGCAGAAAATGAAGAATATCTTGTGGATTGGATCTTAGAGCTTTGGGAAAATGGGAAAATTT TCGATGCAGCAGAGGAAAGTATACGTCAAGAACAAAACAGGGGACAAGTTGAGCTTGTTTTGAAGCTAGGTGTGTTGTGT TCGCATCAAGCTGCATCGATAAGACCGGCTATGAGTGTGGTAATGAGGATTTTGAATGGAGTTTCACAGCTACCAGATAA TCTTCTTGATGTTGTAAGGGCTGAGAAATTTAGAGAATGGCCTGAGACATCAATGGAATTACTACTACTTGATGTGAATA CATCGAGTTCATTGGAGTTAACCGACTCTTCTTTTGTCTCCCACGGCCGCTGAAATTTTAAGTTTACCACTGATTCTTTG TTTGCTATGAAGATGTATCACAACTGAGATTATTACACATTGTAATACACAGCCGAATGTTTTTTTTCCTGG ..............N..............N.................................................. ...............................i................................................ ......i...N...............N........................................i............ .........................................................N...................... ................................................................................ ................................................................................ .......................i........................................................ ................................................................................ .................N........N..................................................... ...N..N........N........................................N....................... ...................N............................................................ ...............................N................................................ .....N...............N.......................................................... ................................................................................ ......................................................N.............N........... ........................N....................................................... ............................N.............................N................i.... ................................................................................ .....................N.......i......i........................................... .....................................N.................................i........ ................................N..............N....i...N....................... ............N................................................................... ...............................N......................N......................... ..........................N.........................................N........... ...N............................................................................ ..............................i...........N............N........................ ........N...........................i...............N..................N........ ................................................................................ ......N.....N...........................................N............... Pos Score Pred ------------------------ 15 0.221 - 30 0.384 - 112 0.674 Yes 167 0.515 Yes 171 0.179 - 187 0.141 - 228 0.755 Yes 298 0.434 - 504 0.714 Yes 658 0.076 - 667 0.260 - 724 0.137 - 727 0.088 - 736 0.351 - 777 0.446 - 820 0.215 - 912 0.402 - 966 0.282 - 982 0.324 - 1175 0.483 - 1189 0.378 - 1225 0.248 - 1309 0.156 - 1339 0.246 - 1356 0.545 Yes 1462 0.258 - 1470 0.634 Yes 1477 0.517 Yes 1558 0.265 - 1592 0.788 Yes 1633 0.191 - 1648 0.396 - 1653 0.808 Yes 1657 0.299 - 1693 0.282 - 1792 0.411 - 1815 0.492 - 1867 0.454 - 1909 0.388 - 1924 0.259 - 2031 0.537 Yes 2043 0.323 - 2056 0.347 - 2089 0.146 - 2117 0.695 Yes 2133 0.296 - 2152 0.214 - 2247 0.043 - 2253 0.060 - 2297 0.042 -

Explain the output -- Processing locus 118: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGCCTCAATTACAATCATAATTCATAAATAATCTTCTGACACTTAATAAGATAACTTTTCACCGAGTGATATATTCCTG AAAGTAAATTCAACGCTTTAGAATAATGTTCTTATATATTTAATTATTTACCAAATACACACTATGCTTCAAACTCCCTA TAAAAAGAAGAAACCACCTTATAGCACTTCTCCTCATTCCTCACTTCCAACCGCCAAAGAAAAAAATCAAAAACTCCAAC AAAAAAAACCTCCCAAACCCTACCCGAGCAGTCGCCAGATGGGCACGGAGGACTACACATTCCCTCAAGGAGCGGAGGAG CTTCACCGCCGCCACCACACGGTGGAGGCTCCACAACCTCAACCGTTCTTGAAGTCACTTCAGTACTCAGTGAAGGAAAC TCTGTTTCCAGACGACCCTTTTAGACAATTTAAGAACCAAAATGCATCAAGAAAATTTGTGTTAGGCCTCAAATACTTCC TCCCGATTTTCGAATGGGCACCACGCTACAATCTCAAGTTCTTCAAATCAGATCTCATCGCCGGAATCACCATCGCTAGT CTCGCCATCCCTCAGGGCATCAGTTACGCCAAACTTGCTAACTTGCCCCCAATTCTTGGCCTTTATTCGAGTTTTGTACC GCCATTGGTATACGCGGTGCTAGGGAGTTCAAGGGACTTAGCGGTGGGAACGGTTGCGGTTGCGTCTCTGTTGACAGGTG CGATGCTGAGCAAAGAAGTTGATGCTGAGAAAGATCCTAAGCTTTACCTTCACCTTGCTTTCACCGCCACTTTTTTCGCC GGCGTTCTCGAAGCCTCTCTTGGAATTTTCAGGTTAGGGTTCATAGTGGACTTTCTATCGCATGCAACGATAGTAGGATT CATGGGAGGAGCAGCGACGCTGGTGAGTCTGCAACAGCTTAAGGGTATATTCGGACTTAAACATTTCACAGACTCTACCG ATGTTATCTCTGTCATGCGTTCCGTTTTCTCCCAAACTCACGAGTGGAGATGGGAAAGTGGCGTTCTTGGATGTGGTTTT CTATTCTTTCTTCTCTCCACCAGATATTTCAGCATCAAGAAACCAAAATTCTTTTGGGTGGCGGCGATGGCTCCTTTGAC CTCAGTGATTCTTGGAAGTCTCTTGGTTTACTTCACTCACGCTGAGAGACATGGTGTTCAAGTGATAGGGGACCTGAAGA AAGGGTTGAATCCACTCTCCGGTTCTGATCTCATCTTTACTTCCCCTTACATGTCCACAGCTGTCAAAACTGGCCTCATC ACTGGCATCATTGCTCTCGCTGAAGGAGTAGCAGTGGGGAGGAGTTTTGCGATGTTCAAGAACTACAACATAGACGGGAA CAAAGAGATGATAGCGTTTGGAATGATGAACATCGTTGGTTCCTTCACATCTTGTTACCTCACAACCGGACCATTTTCAA GGTCGGCAGTGAACTACAACGCGGGTTGCAAGACCGCAATGTCCAACATAGTGATGGCGATTGCGGTTATGTTCACACTC CTCTTCCTCACACCGCTTTTTCACTACACACCACTCGTCGTCCTCTCTGCCATCATCATATCCGCAATGCTCGGACTCAT TGACTATCAAGCTGCCATCCATCTCTGGAAAGTTGACAAGTTCGACTTCCTCGTCTGCATGAGCGCCTACGTTGGGGTCG TATTCGGCAGTGTAGAGATTGGACTCGTCGTAGCGGTGGCGATATCTATAGCGAGGTTGTTGCTGTTTGTGTCGAGGCCA AAAACTGCGGTGAAGGGAAACATACCAAACAGCATGATCTATAGGAACACTGAGCAGTACCCATCATCAAGAACCGTTCC TGGTATTCTCATCTTGGAGATTGATGCTCCCATCTACTTTGCTAATGCCAGTTACTTGCGTGAAAGAATCATAAGGTGGA TTGATGAAGAGGAAGAGAGAGTTAAACAATCAGGAGAGAGCAGCTTACAATATATTATACTCGATATGTCAGCTGTTGGT AATATCGACACAAGCGGTATTAGCATGATGGTGGAAATTAAGAAAGTCATTGACAGGAGAGCGTTAAAGTTGGTATTGTC AAATCCAAAAGGAGAGGTCGTGAAGAAATTAACCAGATCCAAATTCATCGGTGATCATTTGGGCAAAGAGTGGATGTTCT TAACGGTAGGAGAAGCAGTGGAGGCTTGTAGCTACATGCTTCACACGTTTAAAACCGAACCGGCCTCCAAAAACGAGCCT TGGAACAACGTATAGTTCCTTTATTTTTTGCTGTCGTTCTGTGACTACTCGTTATTAACTAATCCGATGTAAACTTAAAT TAAAAACTGAAACTCATTTTGTAATATTGATGAT ................................................................................ .........................N.....................................N................ ................................................................................ ......................................i......................................... ................................................................................ .........................................i...................................... .............N.................................................................. ................................................................................ ................................................................................ ..i..................N.......................................................... .............................................................N.................. N.............N..................................N....................N......... ..................................................................i............. ..................................................N............................. ..................................................i............................. ...................................................i............................ .......i..............i..i...................................................... ......................................i..............i..............N........... ..................................................................i............. ..........................................................N..................... ................................................................................ .................................N.............................................. .......................N....................N................................... ...N.............................................................i.............. ........................N..N.................................................... .........................................................................N...... ...................................N............................................ ..................................................................N............. .............................N.... Pos Score Pred ------------------------ 106 0.156 - 144 0.389 - 279 0.778 Yes 442 0.741 Yes 494 0.327 - 723 0.635 Yes 742 0.194 - 862 0.221 - 882 0.592 Yes 961 0.362 - 975 0.265 - 1010 0.416 - 1031 0.421 - 1107 0.621 Yes 1171 0.305 - 1251 0.718 Yes 1332 0.652 Yes 1368 0.604 Yes 1383 0.549 Yes 1386 0.674 Yes 1479 0.580 Yes 1494 0.627 Yes 1509 0.248 - 1587 0.713 Yes 1659 0.359 - 1794 0.364 - 1864 0.335 - 1885 0.345 - 1924 0.310 - 1986 0.603 Yes 2025 0.226 - 2028 0.185 - 2154 0.421 - 2196 0.060 - 2307 0.062 - 2350 0.037 -

Explain the output -- Processing locus 119: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCTAAGAACCTCCTGTATTGCTAGGTTGGCCATCTTGTGACGAGACGACGTACTTATGAAGTTCTTCTCTTCCTGCGAT TTTTAGTCAGGAAGGAGTTGAACTAAGATTAAGATGGAGATATCAGCTCTTCTTACATCTGCTGGAATTAATATTTCGAT ATGCATAGTTCTTTTATCACTTTACTCCATACTTAGGAAACAGCCAGCCAATTACTGTGTATATTTTGGCCGAAGGCTTG TTTGTGGTGGTGCTAGACGTTATGATCCTTTTTGGTATGAGAGGTTTGTGCCGTCTCCAAGTTGGCTTGTGAAAGCATGG GAGACTAGTGAAGACGAGTTATTAGCTGCTGCTGGTCTTGATGCTGTCGTCTTCCTTAGGATGGTCATTTTCAGCATTCG TATTTTCTTTATAGTTGCTGTTATTTGCATTGCCTTTGTGCTGCCTGTTAATTATTATGGGCAACCGATGGTGCATAAGG AAATCCATTTGGAGTCATCCGAAGTGTTTACAATTGAAAATCTTAAAGAAGGCTCAAAATGGCTGTGGGTTCATTGTCTT GCACTGTACATTATAACCTCAGCGGCATGTCTTCTGCTCTACTTTGAGTATAGCACCATAGCTAAAATGAGGCTTGGGCA TATTACTGGATGTGCCTCAAAGCCAAGTCAATTTACCGTTCTTATCCGTGCTATTCCATGGTCTCCTGAGCAATCTTACA GCGACACGCTGAGCAAATTCTTCACAAACTACTACTCGTCCAGCTACGTGTCCCACCAAATGGTCTACCATAATGGCATC ATTCAGAGACTGCTGCGTGATGCGGAGAGGATGTGCCAGACTCTAAAACACGTTTCTCCTGAAATCAATTGTAAACCGAG TTTAAGGCCATGCACCTTTTGTGGAGGACCTACAGCCACAAGTTCTTTTCATATCCTATCGAATGAGGCAGACAGTGTGA AAGGAATGGAGCTTGGTGAGTTGACTATGACTACGACTACGACAGAGCAAGAACGTTCAGCTGCTTTTGTGTTTTTTAAG ACTCGCTATGATGCTCTTGTTGTTTCGGAGGTTCTACAATCATCAAATCCTATGTTATGGGTGACAGACTTAGCCCCAGA ACCTCATGATGTGTACTGGAAGAATCTCAACATTCCTTATCGACAACTTTGGATACGGAAAATAGCAACCCTTGTTGGTG CAGTTGCTTTCATGTTTGTGTTTCTTATTCCCGTTACCTTCATTCAAGGGCTGACTCAACTAGTGCAGCTGTCTCACGCA TTTCCTTTTCTAAGAGGCATCTTGAGCAAGAACTTTATAAACCAGGTCATCACAGGGTACTTACCCAGTGTGATCTTGAT TCTATTTTTCTATGCCGTTCCACCATTGATGATGTATTTTTCGGCCTTGGAGGGATGTATTTCACGGAGTATAAGGAAGA AGAGTGCTTGCATCAAAGTCTTATATTTTACCATTTGGAATGTGTTCTTCGTGAATATATTATCAGGGTCTGTTATCAGG CAACTGAATGTCTTTTCTAGTGTCAGAGACATACCTGCACAACTTGCTAGAGCAGTGCCAACACAGGCTGGCTTCTTCAT GACCTATTGTTTCACATCTGGTTGGGCCAGTTTGGCTTGTGAAATAATGCAGCCTATGGCTCTTATCTGGAATCTGGTAG CAAAAGTTGTTACCAAGAACGAGGATGAGTCATATGAAACACTTAGGTTCCCATACCACACCGAAATTCCTCGGCTGCTT TTGTTTGGGCTCCTGGGTTTCACCAATTCAGTCATAGCACCACTGATACTGCCGTTTTTGCTGATATACTTTTTCCTTGC ATATCTCATATACAAAAATCAAATACTCAACGTGTATATTACGAAGTACGAAAGCGGTGGACAATACTGGCCTATTTTCC ACAACACAACAATCTTTTCACTGATCTTGACACAGATTATAGCTTTGGGGTTTTTCGGATTAAAGCTATCAACAGTTGCT TCGGGTTTCACCATACCGTTAATTCTTCTCACGTTACTTTTTAGTGAGTATTGTCGGCAGAGGTTTGCGCCCATTTTCAA CAAAAATCCTGCCCAGGTTCTCATAGATATGGACAGAGCTGATGAAATATCAGGAAAGATGGAAGAGTTACATAAAAAAT TGCATAATGTATACTCGCAGATACCATTACACTCTCAGAAATCATCGAGTAAAGCTGAATGTAGTAATCCTTTCAAAAAG CAGGAGTTACCAGATCCTGAAAAATTGAAGCCAGAGGAGGGGGATGCGATAGCTAAAGAGTTATGGGGATTCCAAGGCAA TGAATCTGGTCAAGAACATGACACTAAGTCCTGAGCATCTTTCTCCAAAGAAGATCGAACTTCACAAACGGAACTAGACA GATGTTTTTACTAGTGAAGTGCCTATGTGTTGGTTCAATCACAAAGTCTCCAA ........................................................N....................... .................................i.............................................. N............................................................................... .....................N..............N.......................................N... ........................................N...................i................... ........................................................N..........N............ ..........................................................i..................... ..........................N.......................................i............. .........N...............................................N...................... ...........................................................i............i....... ...................N..........N................................................. .........N....................................................N................. .....i....................N..................................................... .......N..N........................................N....i....................... .....N..N....................................................................... ...........N.................................................................... ................................................................................ ...........N................N..N......................N......................... .......................................N........................................ .......i......................................................................N. ..............................................i........i........................ ........................N........N.............................................. ................................................................................ ................................................................................ ................................................................................ ................................................................................ ............................i............N................i..................... ......N...................................................N..................... ...........................................N..................N................N .................N.............................................................. Pos Score Pred ------------------------ 57 0.238 - 114 0.638 Yes 161 0.297 - 262 0.094 - 277 0.063 - 317 0.268 - 361 0.060 - 381 0.633 Yes 457 0.265 - 468 0.372 - 539 0.821 Yes 587 0.260 - 627 0.728 Yes 650 0.253 - 698 0.226 - 780 0.783 Yes 793 0.558 Yes 820 0.386 - 831 0.312 - 890 0.428 - 943 0.170 - 966 0.680 Yes 987 0.363 - 1048 0.097 - 1051 0.362 - 1092 0.424 - 1097 0.582 Yes 1126 0.182 - 1129 0.183 - 1212 0.476 - 1372 0.443 - 1389 0.139 - 1392 0.202 - 1415 0.398 - 1480 0.195 - 1528 0.583 Yes 1599 0.367 - 1647 0.743 Yes 1656 0.531 Yes 1705 0.252 - 1714 0.199 - 2109 0.576 Yes 2122 0.338 - 2139 0.704 Yes 2167 0.475 - 2219 0.390 - 2284 0.245 - 2303 0.330 - 2320 0.397 - 2338 0.388 - 2402 0.049 - 2425 0.053 -

Explain the output -- Processing locus 120: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTCTGTCTTTGGTCAAATCCACTGTTTAGTATTCAAAACCAATTTTCTCACATTCTATTACACATACATGAATCTCTT CTGCGTCGATTTTCCACGTAATTAACGATAAAAGATCCTATGAAGACTGCAACGTCATTACACACGCACGTACGGATCAG TTTTGTTCCGTTCACACTTCACACTCAGTCAAATAGTCAAACACCTCCACAACCTTCTATAAATACTCGAATCACTTACC ACCAACAATTCATCAAAAACTCGACAATGGAAACTCCCAAAGGCACTATCATTTTCACCACCGTCGGTCGAACTCACTAC GGCTTCGACGTCTTCTCTCTCAACATAGCCACCTCCGTCGAACGCCGTTTAACAGACGGCGTTTCCGTGAACTTCAACGC TCAATTCGTCAACGATAAAAGCGATGATGTCGTCTTTGTTTCGGAAAGAAACGGATCCGCCAGGATTTACAAAACCCGAT CCGGAATTTCTAAGCCCGAGCAGATTCCCGGAGCTCCAGAGAGCTACTTCCACGACCGTCCTATCATCACTCAAAACAAC AGACTCTATTTCATCTCTGCTCACGAGCAACCTGATCGTTATTTCAAGAATTGGTCTGCGCTTTACACCGTGGAGCTCAA CAGCGCAAAGAGAGAAGTTACACGTGTCACTCCACCAGACACCGCCGACTTTAGTCCGGCGGTTTCTCAATCTGGAGATT TCTTAGCCGTTGCTTCTTATGGAACTCGCTCTTGGGGTGGTGAGTTTCACGAGATCAACACTGATATTACTGTTTTCAAA GCATCAAAACCAGAGACAAGAGTGGTTATCTGCGAGCGTGGTGGATGGCCGACTTGGTCCGGCGATTCAACTGTTTTCTT CCACCACCAAGCAGACGACGGTTGGTGGAGCATCTTCCGGGTAGATATACCGGAGAATTTCACGGAATATACCGATTTCC CAATCACACCAATCCGAGTCACTCCATCTGGACTCCATTGCTTCACTCCTGCAGCTTTCCGCGACGGGAAACGAATCGCC TTAGCAACTCGCCGTCGCGGCGTCAACCACCGTCACATCGAGATTTACGACCTTGAAAACACAACGTTTCAGCCGGTGAC TGAGTCACTCAATCCGAGTTTCCACCATTACAACCCTTTCGTGTCTCCAGACTCCGAGTTCCTCGGCTACCACCGATTCA GAGGCGAGTCAACTCAAGGCGAGTCAATCGTACCTAACATCGAATCCATCGTTTCACCAATCAAAACCCTCCGATTACTA AGAATCAACGGATCGTTTCCATCATCATCTCCCAACGGTGATCTAATCGCATTGAACTCCGATTTCGACATCAACGGTGG AATCAAAGTATCTAAATCCGACGGTTCAAAACGATGGACGTTGATCAAAGACCGTACAGCTTTCTACAATTCATGGAGTC CAACAGAGCGTCATGTAATCTACACATCTCTAGGTCCAATCTTCTCTCCGGCGAGAATCGCGGTTCAGATAGCTCGAATC AAGTTCGATCCATCAGATCTCACCGCTGATAAAGAAGATCTTCCATGTGATGTGAAGATTCTCACTCTAGAGAACACTGG GAATAACGCTTTTCCGTCTTGCTCTCCGGACGGTAAATCAATCGTGTTCCGATCTGGTAGATCAGGTCATAAAAATCTCT ACATTGTAGACGCCGTTAACGGAGAATCTAACGGCGGTGGAATACGACGGTTAACGGATGGGCCATGGATTGACACTATG CCTTGCTGGTCTCCGAAAGGAGATCTCATCGGATTCTCATCTAATCGTCATAATCCAGAGAACACGGCCGTGTTCGGTGC TTACGTGGTGAGACCTGACGGTACTGGTTTGAGGAGGATTCAAATTTCGGGACCGGAAGGGTCTGAGGAAGCGGCGAGGG AGAGAGTTAATCATGTGAGTTTCAATAAAGATGGTGATTGGCTTGTTTTCGCGGCGAATTTGAGCGGAGTAACGGCGGAG CCGGTGACGATGCCGAATCAGTTTCAGCCTTATGGGGATTTGTACGTTGTGAAATTAGACGGAACGGGATTGAGGAGGCT GACGTGGAATGGGTATGAAGATGGGACTCCTACGTGGCATACTGCTGATGAATTGGATTTGAGTCAGTTGAATTTGAACG GTCAAGATGGTGACAAGCTTGAAGGTCAGTTTGAGGAGCCGTTGTGGATTTCTTGCGATATCTGAATGCGATTTCTCCTT TTCTTATAAAAGACTGTCATATTTATGTAATCTCGCTTTTTTTATTTCAATAAAACAAACAAAAATAAATGCATTTAGCA TTAT .....................................................................N.......... .......................................i........................................ ................................................................................ ..........................i..................................................... ................................................................................ .......................N..N..................................................... ................................................................................ ................................................................................ ................................................................................ ..................N............................................................. ............................................i................................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ .................................i......................................N....... ............N................................................................... ............................................i....N.............................. ................................................................................ .........................................................N......N............i.. ................................................................................ ................................................................................ ............N.................N................................................. .........N.....................N................................................ ........N.....N.....i..........................N................................ ......N..........................................................N.............. ........................N...........................................N........... .... Pos Score Pred ------------------------ 70 0.180 - 120 0.519 Yes 267 0.911 Yes 424 0.250 - 427 0.476 - 739 0.263 - 845 0.628 Yes 1394 0.565 Yes 1433 0.382 - 1453 0.271 - 1565 0.523 Yes 1570 0.235 - 1738 0.436 - 1745 0.293 - 1758 0.844 Yes 1933 0.225 - 1951 0.151 - 2010 0.415 - 2032 0.173 - 2089 0.429 - 2095 0.175 - 2101 0.505 Yes 2128 0.075 - 2167 0.247 - 2226 0.082 - 2265 0.050 - 2309 0.107 -

Explain the output -- Processing locus 121: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATTCAGACAACGGTTGCGATATTTCTTATGTCTTTTTTTCTATCGTTGTTCATTTCAGAAGTTTCGATATAAAAAAGG TTATTGTTGGATTTTGAGTAACACATTGTCCTCAATTCTCATCGTTCTTCAATCAAATTTTGGATTTTGAGCATCATGGA TGATGGATCTATGCATTTTGATTTTATATTGTACAGAAAGAATGTTTCTTGTTTTGTTTTGATGATGTTTTCTTATGACA ACAATCTTCATGTTTTTGTAGGAGCTTTAAGGTCGGAAATGGCGGCTCATGTTTCTACTGGAAACATTCATAATTTCTAT CTTGCGGGGCAGGTTTACAGGGGACAAGCTTTTTCATGGAGTTCTGCTTCTACTTTCATGGCAAATCCATTCAAAGAGCC CTCTTGGTCAAGTGGGGTATTTAAGGCTCTAAAAGCTGAGAGATGTGGTTGTTACTCTCGTGGTATTTCCCCCATCAGTG AGACCTCAAAACCTATCAGGGCTGTTTCGGTATCTTCTTCAACAAAGTATTATGATTTCACTGTGATTGGTAGTGGAGTA GCGGGTCTGCGTTATGCTTTAGAAGTTGCAAAGCAAGGAACAGTCGCAGTGATTACCAAAGATGAGCCTCATGAGAGTAA CACAAACTATGCTCAAGGTGGTGTTAGTGCTGTGTTATGCCCTTTGGATTCTGTTGAAAGTCATATGCGGGACACTATGG TCGCTGGTGCTCATCTTTGTGATGAAGAAACCGTAAGAGTTGTGTGTACTGAAGGGCCTGAAAGGATTCGTGAACTGATT GCAATGGGAGCATCATTTGATCACGGCGAGGATGGAAATTTGCATTTGGCCAGAGAGGGTGGTCACTCGCATTGTAGGAT CGTTCACGCTGCTGATATGACAGGAAGAGAGATTGAGAGAGCTTTACTTGAAGCTGTACTTAATGATCCCAACATATCTG TCTTCAAACACCATTTTGCAATCGATCTGCTCACTTCTCAGGATGGCTTGAACACAGTTTGTCATGGTGTGGACACTTTG AATATCAAAACTAATGAGGTAGTACGCTTTATATCGAAGGTGACATTGCTTGCTTCAGGTGGAGCTGGGCATATCTATCC ATCAACCACAAATCCTCTGGTGGCTACTGGAGATGGGATGGCGATGGCTCATCGAGCTCAAGCTGTGATCTCAAATATGG AATTTGTGCAGTTTCATCCTACTGCCCTAGCCGACGAAGGTCTTCCCATCAAACTACAAACTGCTAGGGAAAACGCGTTC CTCATCACCGAGGCGGTGAGAGGTGATGGTGGCATCCTCTATAATCTAGGAATGGAGCGATTCATGCCTGTTTACGATGA ACGAGCTGAGCTTGCTCCAAGAGACGTGGTTGCAAGAAGTATTGATGACCAGCTTAAGAAACGAAACGAAAAGTATGTGT TACTTGACATAAGCCATAAGCCAAGAGAAAAGATTCTTGCCCATTTCCCGAACATAGCTTCTGAATGTCTTAAACACGGT CTGGATATCACCCGTCAGCCTATTCCGGTTGTCCCTGCAGCCCATTACATGTGTGGAGGAGTTCGTGCTGGTTTACAAGG CGAAACCAATGTCCTTGGATTGTTTGTAGCAGGTGAAGTAGCATGTACAGGCCTCCACGGGGCAAATCGTCTTGCTAGTA ACTCGCTTTTAGAAGCTCTGGTTTTCGCGAGACGGGCTGTTCAGCCTTCGACTGAGCTCATGAAACGCACAAGACTTGAT GTATGCGCATCAGAGAAATGGACAAGGCCTGTTGTTGCGACAGCTAGATTGCTAGGAGATGAAGTAATAGCAAAGATTAT AGCTTTGACTAAAGAAGTGAGAAGAGAGCTTCAGGAGGTAATGTGGAAGTATGTTGGTATTGTCAGATCGACAATTCGGC TCACCACTGCTGAGAGGAAAATCGCAGAGCTAGAAGCAAAATGGGAAACATTTTTGTTTGAACATGGATGGGAACAAACA GTGGTAGCTCTTGAAGCTTGTGAGATGAGAAACTTGTTCTGTTGCGCTAAGCTTGTGGTGAGCAGCGCGTTAGCTAGACA TGAAAGCAGAGGTCTTCATTACATGACAGACTTTCCTTTTGTGGAAGAAAGCAAGCGGATTCCGACGATTATTCTACCGT CTTCTCCTACAACAGCTAGTTGGAGCTCAAGGCGGTTACAGAATATAAGTAGCAGCTCACTTATTGATTGCTAAAACTGC AGGAGTTTTTGTTCTTATTTGAATCCAAACCTCTCTGATTATTGTATCAATTTGAAAAAATTTGGATCTCAAAAGGAATC TCTGATTCTGACA .............................N.................................................. ...........................................................................N...N ..N.......N..............................N...................N..N.........N..... .........N............................i.........N............................... ...................................N.....................N...................... ..........................................N..................................... ...................................................N............................ .............N...............................................N........N......... ........N...........................N...........................i...........i... .....................N.......................................................... ...i...........................N................................................ ................i.............................................N................. ..........................................N....................N................ .............N.................................................................. ................................N....i.....N................................i... ................................................................................ .........................N.........................i...........i............N... ............................................N.............................N..... ................................................................N............... ................................................i............................... ........N.................................N..................................... ...........................................................i..................N. ..N..............i........................................N..................... ........................................N.........N............................. ........................................i......................i...i............ ........................N......................................................N ......................N......................................................... ................................................................................ ................................................................................ ............. Pos Score Pred ------------------------ 30 0.290 - 156 0.168 - 160 0.267 - 163 0.152 - 171 0.256 - 202 0.270 - 222 0.361 - 225 0.423 - 235 0.120 - 250 0.320 - 279 0.720 Yes 289 0.174 - 356 0.310 - 378 0.458 - 443 0.251 - 532 0.216 - 574 0.120 - 622 0.224 - 631 0.310 - 649 0.389 - 677 0.288 - 705 0.642 Yes 717 0.603 Yes 742 0.183 - 804 0.632 Yes 832 0.289 - 897 0.654 Yes 943 0.157 - 1003 0.376 - 1024 0.382 - 1054 0.212 - 1153 0.193 - 1158 0.734 Yes 1164 0.423 - 1197 0.857 Yes 1306 0.213 - 1332 0.684 Yes 1344 0.531 Yes 1357 0.168 - 1405 0.253 - 1435 0.490 - 1505 0.389 - 1569 0.770 Yes 1609 0.231 - 1643 0.373 - 1740 0.525 Yes 1759 0.167 - 1763 0.400 - 1778 0.791 Yes 1819 0.108 - 1881 0.419 - 1891 0.455 - 1961 0.582 Yes 1984 0.516 Yes 1988 0.579 Yes 2025 0.493 - 2080 0.209 - 2103 0.454 -

Explain the output -- Processing locus 122: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTACGTTTCTCATATGGTTCTCATTCAACGACTTTCCAAGTCGGACTATATATGTTTTTAATTATTAATCATATATAGAG ACGCTCCTACAAAACACATAAACATATAGGTGCATGCATATAAATGTAAATGTATTGTTGTCGTCTACTTCAATAACAAC TCCATCAATGAACACATCAATACTTGCCATACTTTTTCTCATACAATGTGTTTTCACTCTTGGTCTCCATTTCCACCCAC TTGATCCCTTAACTCCCCAAGAAATCAACAAAACAAGCTTTATCGTCAAGAAATCTCATCTAGGCAACCTCAAAGATCTC ACATTTCATTATCTCGACCTCGAAGAACCAAACAAGAGCCACGTCCTCCAATGGCTATCACCAAATCCCTCCAAGAAACC ACCACCCCCACGTCGTCGCTCATTCGTCGTGGTTCGAGCCGGTGGTCAAACCTACGAGCTCATCATCGACCTAACCACGA GCAAGATTGCATCCTCCCGCATCTACACAGGCCATGGTTTCCCCTCATTCACATTCATAGAGCTTTTCAAAGCAAGCAAG CTTCCTCTAACTTACCCACCTTTCAAGAAATCGATTCTCGATCGATCCCTAAACATCTCCGAGGTTTCTTGCATCCCTTT CACCGTTGGTTGGTACGGAGAAACCACCACGAGACGTGAACTCAAAGCCTCTTGCTTTTACAGAGACGGATCAGTCAACG TCTTCACAAGACCCATCGAAGGAATCACCGTAACTATAGACGTCGACTCAATGCAAGTCATCAAGTACTCCGACAGATTC CGAAAGCCTATCCCTGATAAAGAAGGTAACGACTTTAGGACTAAACACAGACCCTTCCCGTTCTTTTGCAACGTCTCCGA CACAGGCTTCAAGATACTCGGCAATAGAGTCAAGTGGGCTAACTGGAAATTCCACGTCGGATTTACCGCAAGAGCGGGAG TAACTATATCGACGGCTTCGGTTCTTGACCCGAGAACCAAAAGGTTTCGACGAGTGATGTATAGAGGGCACGTGTCAGAG ACTTTTGTTCCGTACATGGACCCAACCTACGAATGGTACTACCGTACGTTCATGGACATTGGAGAGTTTGGTTTCGGGAG ATCCGCCGTGAATCTGCAGCCACTCATTGACTGCCCGCAAAACGCTGCGTTTTTAGATGGGCACGTGGCCGGACCAGATG GGACAGCTCAGAAGATGACCAATGTGATGTGTGTATTCGAGAAAAACGGTTACGGTGCTTCTTTTAGACACACCGAGATT AATGTTCCAGGACAAGTGATAACAAGTGGGGAAGCTGAAATAAGTTTAGTGGTTAGAATGGTGGCCACACTTGGAAACTA TGATTACATAGTGGATTGGGAATTTAAAAAGAATGGAGCCATCAGAGTTGGGGTGGATTTGACTGGAGTTTTAGAAGTAA AAGCGACATCGTACACTTCAAACGATCAAATAACGGAGAACGTATACGGTACACTGGTGGCGAAGAACACTATCGCCGTT AACCACGACCATTACCTAACGTACTACTTGGACCTCGACGTTGACGGTAACGGCAATTCCTTGGTGAAAGCCAAACTCAA AACGGTAAGAGTAACGGAAGTTAACAAAACGTCTTCTCGGAGGAAGAGTTACTGGACGGTCGTTAAAGAGACGGCGAAAA CGGAAGCTGACGGTAGAGTTCGACTCGGCTCGGATCCGGTTGAGCTGTTAATAGTAAACCCGAACAAGAAGACGAAAATA GGAAATACAGTTGGTTACCGGCTGATACCGGAACATTTACAAGCCACTTCGCTTTTAACCGATGATGATTACCCCGAGTT AAGAGCCGGTTACACGAAATATCCGGTTTGGGTGACCGCCTATGACCGGTCCGAGAGATGGGCCGGTGGGTTTTATAGTG ATCGGAGCCGTGGCGATGACGGTTTAGCTGTATGGAGTAGTAGGAACAGAGAGATAGAGAACAAAGACATAGTGATGTGG TACAACGTTGGGTTTCATCACATTCCATACCAAGAGGATTTTCCGGTTATGCCAACTCTTCACGGTGGTTTTACTCTTCG GCCTTCAAATTTCTTCGATAACGATCCTTTGATTGGGTAAATACGGTCTTAGACAAACAAACTGATGGCTTATGTATTGA TTGACTTATGTTACGTCAAACATATGATCATTGAAAAAACAATTTTTAGCCTTGTGACT .............N.....................................N............................ .................................N.........N.....N.............................. .......N.....................................i.................................. ................................................................................ ..................................................i............................. ................................................................................ .................................N.............................................. ................................................................................ ................................................................................ ..................................................N............................. ................................................................................ ................................................................................ ........................................................N....................... ...............N................N..................i............................ ........................................................N....................N.. ..............i......N....i..................................................... ................................N............................................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ .............................................................N..N............... .........................................i...............N...................... ...............N...............N..........................................N..... ................................................N............................... ................................................................N......N........ .......N...............N................................... Pos Score Pred ------------------------ 14 0.333 - 52 0.175 - 114 0.118 - 124 0.325 - 130 0.269 - 168 0.456 - 206 0.546 Yes 371 0.597 Yes 514 0.446 - 771 0.434 - 1017 0.379 - 1056 0.444 - 1073 0.339 - 1092 0.514 Yes 1177 0.425 - 1198 0.339 - 1215 0.683 Yes 1222 0.238 - 1227 0.515 Yes 1282 0.160 - 1338 0.745 Yes 1360 0.121 - 1393 0.274 - 1822 0.340 - 1825 0.195 - 1882 0.690 Yes 1898 0.299 - 1936 0.397 - 1952 0.115 - 1995 0.373 - 2049 0.478 - 2145 0.219 - 2152 0.102 - 2168 0.052 - 2184 0.073 -

Explain the output -- Processing locus 123: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTATGTTTTCTCTCTTTTGTCCCCTTTCGATTTCCTTTTCGCTATTTTTCTGATACATAGGTGTTGATTGTCCTCTCGT GTTTTGTCTTACAACGGCTAAAGTGTCCTAATGAAAGGTCTGAAAAAAGATTATATGAGCGATTGGTGTATTTGGATAAT GATAAGGGCTGCAATGCATTCTTCCTGTATTCAATTCTATAGATGTTTTAATTTGTGGGCTTGCTTCAAGTATTTGCCTT TATTTCGATTTCGGTTTAATCGAGCCTTCTCCGAAATTGCGCATCCGTTTTTGTGTTCTTTAGCTCCATTGATGATATAT GGGTTATGGTTGTGTGCAGATAGCTGTTTATTCGAAATAAAGAAAATGGAAGGAGGGTCTGTTAATGAATCGCATTCTAA TGCGGACCAAATGTTTGACACAACTATAGAAGAGTTATGTAAGAATCTCTGCGAATTGCAGAGTTCTAATCAATCTCCTT CAAGGCAGAGTTTTGGATCATATGGTGATGAGTCAAAGATTGATTCTGATCTACAACATCTTGCTTTGGGAGAGATGCGA GATATTGATATCTTGGAAGATGAAGGAGATGAGGACGAAGTGGCAAAGCCAGAAGAGTTTGATGTCAAGTCAAATTCTTC AAACTTGGATTTGGAAGTCATGCCCAGGGATATGGAGAAACAAACTGGTAAGAAGAATGTGACTAAATCGAACGTTGGAG TCGGTGGAATGAGGAAGAAGAAAGTCGGAGGTACCAAGTTACAAAATGGAAATGAAGAACCATCATCTGAAAATGTAGAG CTGGCACGTTTTTTACTGAACCAAGCGAGAAACTTGGTTAGTTCTGGTGACAGTACACACAAAGCTCTCGAGTTAACTCA TCGAGCAGCTAAGTTGTTTGAGGCTTCTGCAGAGAATGGGAAACCATGTTTGGAATGGATCATGTGTTTGCATGTTACAG CAGCAGTCCATTGTAAGTTAAAAGAGTACAATGAGGCAATTCCGGTTCTACAGCGCTCTGTTGAGATTCCTGTTGTTGAA GAAGGTGAAGAGCATGCTCTAGCTAAATTTGCTGGTCTGATGCAGTTAGGTGACACTTACGCTATGGTGGGGCAGCTAGA GAGCTCGATATCGTGTTATACCGAGGGATTGAATATCCAGAAGAAGGTTTTGGGAGAAAATGACCCAAGAGTTGGTGAAA CATGCAGATACCTAGCAGAAGCTCTTGTACAAGCATTACGGTTTGATGAGGCTCAACAGGTCTGCGAAACGGCTCTTTCT ATACATAGAGAAAGCGGTTTACCAGGTTCAATCGCAGAGGCCGCGGATAGAAGACTCATGGGGCTTATATGTGAGACTAA GGGAGATCATGAGAATGCCCTTGAGCATTTGGTATTAGCCAGTATGGCCATGGCAGCAAATGGACAAGAGTCTGAGGTTG CTTTTGTTGATACTAGCATTGGTGACTCGTACTTGTCTTTGTCTCGGTTCGATGAAGCGATTTGTGCATACCAGAAATCT CTCACAGCACTGAAGACAGCAAAGGGAGAGAACCATCCAGCTGTTGGTTCAGTTTATATCCGTTTAGCTGATCTCTATAA TCGGACAGGAAAAGTGCGTGAAGCAAAGTCTTATTGTGAAAATGCTCTTCGGATATACGAGTCTCATAACCTAGAGATTT CTCCTGAAGAGATTGCAAGTGGTCTTACAGATATCTCCGTGATATGCGAGTCTATGAACGAGGTGGAACAAGCTATTACC TTGCTACAAAAGGCGCTGAAGATATACGCGGATTCTCCTGGCCAGAAAATCATGATTGCAGGGATTGAAGCTCAAATGGG AGTGTTGTACTATATGATGGGGAAATACATGGAGTCGTACAACACATTTAAGAGCGCTATCTCAAAACTACGGGCGACTG GAAAGAAACAATCAACGTTTTTCGGGATTGCACTTAACCAAATGGGTTTAGCATGCATTCAGCTCGATGCAATAGAAGAG GCTGTTGAGTTGTTTGAAGAAGCCAAGTGTATTTTAGAACAAGAGTGTGGTCCTTATCACCCGGAAACACTTGGATTGTA CAGTAACCTTGCAGGAGCTTATGATGCAATTGGCAGGTTGGATGATGCGATTAAGCTGCTAGGACATGTGGTTGGGGTTC GGGAGGAAAAGCTCGGGACAGCGAATCCTGTAACGGAAGACGAGAAGAGGAGGCTGGCCCAACTCTTGAAAGAAGCTGGT AACGTTACAGGAAGGAAAGCTAAATCCCTCAAGACTCTAATTGATTCCGATCTCACTTCTTCTTCAGCTCTTCGTTAACA ATATGTTACATTTTCTTATTATTTTATAGAAATGTGAATACGATATTTTTGTTTTTTGTGATTATGTTTGTGGAAGTGTT TTCCTTTCATTGTCTTA ...N............................................................................ ..............................N.......................N.......................N. .............N............................N..................................... .......................................................................N......i. .....N.......................................i..................N..............N ..........N.........................N........................................... .....................N.....N..............................................N..... ...................N........N................................N.................. ...................i...........i........................N....................... ........N....................................N.....N....................N....... ................................................................................ ...................................N.........N........N......i.........N........ ..............................N................................................. .............N.........................N.......................i................ ...........................................................N.................... .........................................................i..........N........... ........N.....N............................i.....i.........N.................... ...................................................N............................ ................................................................................ .........................................N...................................... ...........................................i.........i.......................... ...................................................N.......................i.... .............N..N...........N................................................... .........................................i..........N.............N............. ................................................................................ ....................N..N.................N..N....................N.............. ................................................................................ ................................................................................ ..N............................N...............................N................ ................. Pos Score Pred ------------------------ 4 0.374 - 111 0.311 - 135 0.156 - 159 0.148 - 174 0.411 - 203 0.275 - 312 0.259 - 319 0.604 Yes 326 0.261 - 366 0.624 Yes 385 0.434 - 400 0.303 - 411 0.436 - 437 0.346 - 502 0.217 - 508 0.290 - 555 0.471 - 580 0.268 - 589 0.165 - 622 0.107 - 660 0.567 Yes 672 0.572 Yes 697 0.354 - 729 0.470 - 766 0.394 - 772 0.179 - 793 0.296 - 916 0.427 - 926 0.498 - 935 0.303 - 942 0.631 Yes 952 0.198 - 991 0.278 - 1054 0.127 - 1080 0.212 - 1104 0.769 Yes 1180 0.152 - 1202 0.503 Yes 1246 0.359 - 1338 0.509 Yes 1349 0.256 - 1369 0.202 - 1375 0.133 - 1404 0.573 Yes 1410 0.675 Yes 1420 0.488 - 1492 0.159 - 1642 0.196 - 1724 0.573 Yes 1734 0.624 Yes 1812 0.213 - 1836 0.513 Yes 1854 0.135 - 1857 0.296 - 1869 0.264 - 1962 0.580 Yes 1973 0.433 - 1987 0.306 - 2101 0.199 - 2104 0.180 - 2122 0.267 - 2125 0.235 - 2146 0.223 - 2323 0.184 - 2352 0.058 - 2384 0.071 -

Explain the output -- Processing locus 124: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATACCTCAAAGTTATTTTCTAATGATATTTATAAGCTTAGAGTTTTCCCTACCAAAAAAAAGAAGCTTAGAGTTTTTT CTTATAAGTTATAGTAATTAATATCGACTAATGTTTTTGTTATTTGATTTAATTCGTTGGAAGCTAGAACGGTCTCTTCT TTCTTATTTACCGTTTTACCCTTCGTATTTTGTGAATGTAACAGAGTTCCCCGACAACGACGTCGTTTCAGGCTCTACTA TAAAAAAACGACGCTGTTTTTTTCAAGAAGATTATCATTCGTCTCTCGTCGTCTTGGAAAAAGATTCTTTGAAAGTCCCG CCCAAATTTAAGACGACGATGACTAATCCAATGGAAGTGGACCGAATCAGCGATGAAACTGGAGCATATCGAGAAGAAGC ACTCTATTACCTCGAAGCATACGACTGGAATTTAGCTCCGGCGATGGAAGCTTGCGGCGTCAAAACCTTGCCTTCCGTCA AGGTCAAATCTTCACCGGTAGTTTCAGTTACAGAGCAGTCCACGGCGGAGGAATATTCAGCTACTCCATTAACGATAATT CCACCTCGGGTTTCGAATCAGGATCCAAGATCGATCAATTATCCAAGTCTGACTTCGAATCCGTTGCCGCCAAGAAAAAG AAGCCATTCAGGACGGGTTTTGAATCCGCCGCAGGAACTATCGCATGAATTGAAGCAAGAACGAATCAATCTATTCCTAG CAGTGGCTTCTGATTTGCCTTCACAAGAAGTCCTCTCTTATCTCGAGCGCTTCAATTGGGACGTGGAGAGAGCCGGGGCC CAATACTATGAGGATCGAGATCAGCAGCGTACTTCATATGATCGTACGGAGATTGATCAGTCGTATTTGCAGCGGTTTCA AGAGGGTTATACGCCGATGATTGGGGTTTCGAATTTGGAAGGATTCTCACAAAATGAAACATCGACTATGGATTTGATGC AGCATAGTGCTCCGCCTCTTCCATCGTTACATTTACCTTCAGAATCTCAGTTTCATGATTCTGTTGGTTATCAATTAGGG GACCACTCTTCTTCTTTTTCTGGATTGATGAACGTTGATGTGGATGAGTTTGGAGATGTATACGATATACCTATAATGTC TTCATCGTCTCAAGTTCAGGTACTAATCTTCTCTTTCTCTGCACTATATCTTTCTTCTGGTTTGCTCTGTTTCCTTAAGA TAGTCTTTACAAAGAAGGGAACTAACTCTATGTTGATTGATGCACATAGAAGCTTTGTGTTTGATGATGAAGGAACTTTA GAGAAAACGAGAATTCAGGGAAGTAGCAGTTCTGCTCAAAACTTGGAAGACCACCCACCGCCAAACAATATTGATAGAAT CTGCACTGAAACTGGTGTGTCTCGAGGTGAAGCAGTCTATTACCTTGAATCCTTCGATGGTGATTTGGCTTCAGCGATAG AGGCTTGCCGCAGCAAAAACTTGCCTACCCTCAAGGTTAGATCTTCGCCAGTGGTTTCGGTGAATGAGATATCCGCGGTA CCAGAGTGGGAGTCACCAACAGAGAGGCCACTGGTGATGAATTCTTGTCCGATTTCGAATGAGCTGCAGATTCAGCCAGA GCAACTACCGCTAAACAAAGAGGAAATCATCAAACAATTCTGTGAGATTGTTGGATTGCATCCAGATGTCGCAGTCGCTT ACCTCGAGCGTTGCCAATGGAGTTTACAAGTTGCCATCGACTATTTCATGAATGAGGCATATTTCACAGAGGATGTAACA TCCAACCCGCCTCTTCCATTGATAGGACTACCTTCACAATCACAGTTTCAGGCTTCGGGGAGCTCTTTTTCTAGTTTGAC GAACATTGACCCGACTGATTTCACCGTAGCTAATCTTCCAATGGCATCGTCTCAAGTGGATCAACCTAGATATATCTCTC TTGATGGTCTTTCTTTGGATGTGGCCTTTTTTGTTGTTGAGGAAGATTTAAGCACGGAGACATTTCTTGATCCTCAGGCC ATCCAAGGCTGGACAAGTGTGGGGCATGAAGCTGCACCGACTACAATTACACTAACTATCTACTTGGACGATGGTGGGTC TGTGACACCGGTATATATTCCTTTTAGATCAGACCAAACCGTCAGAGACATCCGCAATGCCATTGACGAGCTAACACCAG CTAACAACAGATATTACGATTTGCGCTCAGTGGGTGGAGAAGATGATTGCAGGGATATGAATGCAACTGTAGGCAAAATT TGTAAGAGTGGTTCTACAACTCTCCACCAGGTCTTCCACAACACTTAGCTTTGGATTTTTACAGTTTCCATTGATTGATG ATGAAACAGATAAGAAAGACAGAGAGATGTATATGATTTTCGAAAGGATTCTTGCCTTGTATAGAAA .......................N........................................................ ..............................i................................................. ...................................N............................................ ................................................................................ ..................i...........i.....................N........................... ...........................................i.................................... ................................................................................ ................................................................................ ............................................N................................... ................................................................................ .......N.............................N.......................................... ................N....................................N.............i........N... ......................................................N......................... ...........................N.........N.....N...........N...................N.... ................................................................................ .............................i.........i.......................N..N............. ................................................................................ ........................................................N....................... ...............................................................N................ ....................................N.....................N..................... .................................................................N.............. ................i..............................N...N....................N....... ................................................................................ ........................................i....................................... ...N..............N............................................................. .........................N............................................i......... ........................................................N....................... ..........................................N.............i...N................... .............................................................................N.. N.........................N.....N.................................. Pos Score Pred ------------------------ 24 0.117 - 111 0.602 Yes 196 0.305 - 339 0.575 Yes 351 0.753 Yes 373 0.358 - 444 0.727 Yes 685 0.144 - 808 0.346 - 838 0.179 - 897 0.368 - 934 0.306 - 948 0.812 Yes 957 0.225 - 1015 0.113 - 1068 0.300 - 1078 0.109 - 1084 0.067 - 1096 0.167 - 1116 0.433 - 1230 0.608 Yes 1240 0.619 Yes 1264 0.203 - 1267 0.486 - 1417 0.140 - 1504 0.299 - 1557 0.220 - 1579 0.245 - 1666 0.245 - 1697 0.681 Yes 1728 0.225 - 1732 0.340 - 1753 0.383 - 1881 0.757 Yes 1924 0.178 - 1939 0.187 - 2026 0.141 - 2071 0.585 Yes 2137 0.489 - 2203 0.211 - 2217 0.688 Yes 2221 0.287 - 2318 0.068 - 2321 0.089 - 2347 0.060 - 2353 0.046 -

Explain the output -- Processing locus 125: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATCATACACACAGGTTTTAACTGGTCTGCTTCATTTCTACGGTGCAGAGTTTGTGTTGGCATTCATTAGTCTCTTTCC GTTAGGCTGTGAGGCTTATGATCGGTGCCACTAACGTTACTGGCTAAGTCATTGAATCTGATTAAAATATGGCCCTTTAT GGTACATTGCAGTTGTCTCATAGTCTGGGTTTGTGCAGAAACCAACGGTTTTGTAATCCAGAAAATGTATGTTTCTTCCA TTTTAGCCCTGACATCCTTTTCTTTTAATCATTTCTTCCCTTCATCTTTCGAAAAATGTACAATTTTTCAGAGTGCCATG AGAAGGAGGCTGCATATATCAAATGGTCCTCTCTCATTAGGTGTTCCGTTGGGACAACATGGTTTCAGCAACATATTACT CTCAAATTACCTTCGTAGGCCTATTTGTTCAGTTCCATGCAGAACCACCGCTTTTAGGTGTCATTCCTTTTCTGCAAGCG GCAAGGCAATTGAACCTGCTGTTAAAGCTGTCACCGTGGTGCTAACAAAGTCACACGGGTTGATGCAACAATTCCCTTTT GTATATAAATTGGTTCCAGCTGTTGCTCTTCTTGTTTTCTCCCTGTGGGGTCTAGTGCCTTTTGCTCGCCAAGGAAGAAA CATACTACTCAATAAGAATGATAACGGTTGGAAGAAGAGTGGTACGTACCATGTTATGACTTCATATGTTCAACCTCTGT TACTATGGTTAGGAGCTCTATTCATCTGCAGGGCATTAGATCCTGTTGTCCTTCCTACAGAAGCTAGCAAAATTGTGAAG GATCGACTGTTGAATTTTGTGAGGTCATTGTCAACGGTACTTGCATTTGCTTACTGCTTATCCAGTCTTATCCAACAAAC ACAAAAGCTATTCTCAGAAACGAGTAATCCGAGCGATACAAGAAATATGGGATTTCAATTTGCTGGGAAAGCATTGTATT CTGCTGTATGGGTTGCTGCTGTATCACTTTTCATGGAGTTGCTGGGTTTCTCTACACAAAAATGGCTCACTGCTGGAGGT CTTGGGACAGTTTTGATTACTCTTGCTGGCCGAGAGATTTTGACAAACTTTCTTTCAAGTGTTATGATTCATGCAACCCG GCCATTTGTTTTGAATGAGTGGATTCAAACAAAGATAGAAGGTTATGAAGTGTCTGGTACTGTGGAGCATGTTGGTTGGT GGTCACCAACAATCATAAGAGGTGAAGACCGCGAGGCAATTCACATCCCAAACCATAAGTTCACAGTTAATGTCGTGAGA AACCTTACTCAGAAAACTCATTGGCGTATTAAAACTCACCTAGCAATCAGTCACTTGGATGTCAACAAAATAAATAATAT TGTAGCTGACATGCGGAAAGTGTTGGCCAAGAATCCTATGGTTGAGCAGCAGAGGTTACATAGAAGGGTATTCTTGGAGA ATGTCATTCCAGAAAATCAGGCCCTCTCGATACTGATCTCTTGCTTTGTGAAGACGTCTCATCACGAAGAATATTTGGGT GTAAAGGAAGCTATATTGTTGGACCTTCTTAGAGTCATAAGCCACCACCGAGCTCGTCTAGCTACACCAATCCGTACAAT CCGTAAGATGTACACAGAGACTGACGTGGAAAACACACCATTTGGGGAATCGATGTACGGAGGTGTTACTTCTCGGCGGC CTTTGATGCTAATTGAACCCGCTTACAAAATCAATGGAGAAGACAAATCAAAATCTCAAAACCGTGCAGCAAAACCAACC GCAGAGCAAGAAAACAAGGGCTCGAATCCAAAGTCAAAAGAAACGTCTTCTCCTGATCTAAAGGCGAATGTGAAAGTTGG AGAATCCCCTGTTTCTGATACCAACAAAGTACCTGAAGAAACAGTAGCAAAGCCAGTAATCAAAGCGGTCTCAAAACCGC CCACCCCAAAGGATACAGAAACATCAGGAACTGAGAAACCAAAGGCCAAGAGAAGTGGTGGCACAATAAAATCAACAAAG ACAGATGAAACAGATAGTTCAACATCATCAGCGTCTAGATCAACTTTAGAAGAGAATATAGTACTGGGTGTTGCACTAGA AGGCTCAAAGAGAACACTTCCAATCGAAGAAGAGATACATTCTCCTCCCATGGAAACAGATGCTAAAGAACTCACGGGAG CTCGCAGATCCGGTGGAAATGGACCATTGGTGGCTGATAAGGAACAGAAAGATAGCCAATCACAACCAAACTCAGGTGCT TCAACCGAGCCGTGAGATGAAATTCAATGAGTTAGCTATTTTTTTATAACACTCTTTGATGTAATATACGATTGAAATTA ATTGAATTTACCCTCCCAAAATTCTGGAAGTTGG ................................................................................ .................N..................................................N.........N. ................................................................N...N........... .......................................................N.....................N.. ......................i...................................i..................... ....................................N........................................... ..............................................................N................. ................................................................................ .................N................................N....N.........N.............. ....N........................................................................... ................................................................................ ..............................................i................................. .......N........................N............................i.................. ...............................................................i......N......... ..............N.............................N.......................N........... .....................................................................N.......... ..........................................................N..................... ..........i..........................N.......................................... N............................................................................... ................................................................................ .......i............................................i........................... .....N...........................N.............................................. ...................................................................N............ ................................................................................ ................................................................................ ....N........................................................................... .................................................i.........N.................... ..................N............................................................. ................N.........N...............................N..................... .................................. Pos Score Pred ------------------------ 98 0.496 - 149 0.406 - 159 0.217 - 225 0.419 - 229 0.133 - 296 0.308 - 318 0.254 - 343 0.620 Yes 379 0.584 Yes 437 0.377 - 543 0.301 - 658 0.289 - 691 0.154 - 696 0.352 - 706 0.154 - 725 0.417 - 927 0.756 Yes 968 0.364 - 993 0.435 - 1022 0.751 Yes 1104 0.510 Yes 1111 0.190 - 1135 0.433 - 1165 0.442 - 1189 0.146 - 1270 0.393 - 1339 0.254 - 1371 0.572 Yes 1398 0.401 - 1441 0.250 - 1608 0.525 Yes 1653 0.596 Yes 1686 0.357 - 1714 0.415 - 1828 0.296 - 2005 0.265 - 2130 0.701 Yes 2140 0.338 - 2179 0.373 - 2257 0.235 - 2267 0.081 - 2299 0.064 -

Explain the output -- Processing locus 126: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGCGACGACAACGATTGATTGTGACCGTAACACATCGTAAAAGGAAAGATAAACACAAAAATCAATTCTTCGGTTTTGG CCTTTTTGGGTTTCTCTCTAAAGGAAGAAGAACATAATGAAGGGTGTTTTGGTCATTTCATTAATGGTCAAAAGTCAAAC TCTTCTTATTAAGTCAGAGGGTTTTATCTGTTATATAAATCAGTCACCGGCGCCGGTGAATGAAGCAAGAACAATGTCGA AGCCTTCTTCTTCACCTTTCTTCTCTGAAACCACTTCTCGTCTCCAAGACGAAACAGTTTTCTTCTCCTTATTCCCTGAC TCATCCCTCTCCTCCGCCGCTCTTCAGTCACTTCACTTAGAAATCATCGATTTCGTTTCTCCGTTTACTTCTCCTTACAT ATGGCAGCACGAGCCTTTCTCTCTCTCAATCGCTCTCTCTTCCTCTTGTGCTTGTACCAATACTGCTATACCTCATCTCC ACGGGAAGCTCAAGTACGGTGACAATCTCGAAGACGAATGGTTCGCTGTGTTTTTACTTTTTCGAATCTCCGCCGCGTTT CCGTCTAATTCGATTCGTGTTTGGGACACTGACGGCGAGTTTCTCCTAATCGAAGCTGCTTTTCATCTCCCGCGTTGGCT TAATCCGGAGACTAGTCTCAATCGTGTCTTCATTCGCGGTGGTGACCTCCACATAGTTCCCAGAAGCCGTTTGCCTGATC CTTCTTTGGTTGCGTCTCTACGGTTTCTAATCGAGCGTGGTAACGAATCTCGTGCGTCGGATTCAGTTCAATCTGCGTTG AAGAATCGGATATCTGATTATCCTGAAAGAGCGTGGAGGAATATGCATCGTGTTAGGGTTAGGGTTCCGGTATCGGTAGC TCAAGTGATTAGGCATGAACCTTTCTTGATCTCACTTGCTGTGGAAGGGTTTTACGATCGTGATATGGATTCAATGAAAC ACGCAGCGAAGATGGAGAAGTTTCTGAGCAAAGGAAGAGAAGAGAAGCTTGTGCTTGTTCTTGTCAAGATGTCTCGTGCT ATGTATGGGCAGCTAGTGCAGCAGAAGTTTCAAGCACCGAATTGTTATCCAATGCCTAGTGTCAGTGACAGGGATGCTTT CTCTGAAGCTGAGCTAGGGATGAAGATAGCTTGTGGTATGGAAATGATGTACCAGCAGAGGAAGAAGGAAGGAGAAGATG GGAAAGGGATTAGTTGGAGCAAGTATAAAGACAATCTTGAGAAGTATGGTTATTTTGAAGGATTGCTTTCTGGTTCGAAG GAGTATAAGAGGTTGATGGAGAATGCTGAGGAATACCACCAGAAGAGTTCTTCGTTTTCGAGGACTAGAGACATTATGAG TGCACCGGTGAGACGTATTGATGAGATTCTTGCTTTACCTTATTCGGAGGATGACTTTAAGGGCCAGGAAGTTCCTGCTT CTGACAATGATTCTTGGCTCTACGATGGTGAAGATGAACTAAACTCTGTTCTTCAAGAGAGGCAGAAAGAGATGGAGTTT TATAACTCAAAGAAGGAACGCAAAAATAAAGGTAAGGAGAAGCAAGAAGCTGGAAGCTCATCTGATGCAAACATGAACAA TTTTGATCTCGGTGATATCTCAAAGTCGATGCAGCAATTCATGCACAAGGTATCAAGCTACAAAGGAGCAGAAGTTCCAG AAAACAGGGATTTCAAAGAAGTGAGTATAGATGTTGATCGTTTCATGAAAGATATTGAGTCAATGCTAGGAAGCCAAGGT CGTGACGAACAAGCTGATGATGACAGTGATGGCTCTGAAGGATCTTCCATGGACATGGATTTTGATGACGTTGAAGATGA TAGCGAAGGAGAAGAATCTAACGAAGATGCTAAGGAAAGCTTTGAGGAATCCTACTATGGAGCAATGAATGAGGAACTGA AAAACTCAACTCTTGAGAAAAGCTTTGAAAATGTGAATCAACAACATTCTTCTAAGCAAAACGAGGAATCATCAAAAACT AGAGATGAGAAGGATGATGAGTTTACACCAGTTGATGCAGATTTTAACCTTGTTAAGAACCTACTTGAATCCTACTCTTC ACAACAAGGACTTCCTGGTCCTGCTTCCAATTTGCTCGGCCTCATGGGTTTGCAGCTTCCAAAAGATTCCGGCGACAAAA ACTGAATGCTTATCAAAATGTAGTTTGTTTTTGGGTTCACTTTGTTTAATCACATAATCGTGAGTAACGTTGTTGGGGAA GTTTTTGTGTTGTCGAAAGTAAGC ................................................................................ ....................................i..........................i................ ...........................................................N.............i...... ................................................................................ ................................................................................ N............................................................................... .....................................N.......................................... ................................................................................ ................................................................................ ................................................................................ ..........................................i..................................... ..............N.................................................i........i...... ...........i........................................................i........... N...N..............................................i.....................N...... ...................i.................N.....N..N..............................i.. .............................................N.................................. ...............N......N....................................................N.... ....................N.............................N............................. ......N.................N........N.....................................N........ ................................................................N.......N....... ............................N...........N....................................... ..............................N.............i.................i................. ................N..N........N...................i.....i.........N...........N... ..........................N.............................N.......i...N........... ..............................N................................................. ....N........N..N.................N............................................. ...........................................N.................................... .....N...........N.............................................................. ........................ Pos Score Pred ------------------------ 117 0.687 Yes 144 0.536 Yes 220 0.182 - 234 0.756 Yes 401 0.500 - 518 0.395 - 843 0.715 Yes 895 0.146 - 945 0.662 Yes 954 0.561 Yes 972 0.555 Yes 1029 0.506 Yes 1041 0.430 - 1045 0.142 - 1092 0.794 Yes 1114 0.132 - 1140 0.587 Yes 1158 0.311 - 1164 0.378 - 1167 0.323 - 1198 0.504 Yes 1246 0.190 - 1296 0.390 - 1303 0.114 - 1356 0.456 - 1381 0.262 - 1411 0.274 - 1447 0.217 - 1465 0.479 - 1474 0.314 - 1512 0.487 - 1585 0.175 - 1593 0.456 - 1629 0.275 - 1641 0.405 - 1711 0.330 - 1725 0.622 Yes 1743 0.590 Yes 1777 0.184 - 1780 0.274 - 1789 0.354 - 1809 0.788 Yes 1815 0.865 Yes 1825 0.294 - 1837 0.314 - 1867 0.109 - 1897 0.208 - 1905 0.857 Yes 1909 0.146 - 1951 0.377 - 2005 0.314 - 2014 0.270 - 2017 0.192 - 2035 0.154 - 2124 0.377 - 2166 0.225 - 2178 0.335 -

Explain the output -- Processing locus 127: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACCTGTTTTTTTTTTTTAAAACTGTATTATAAAAACGTGTCTTGTTAGGCCTTTAAAGCCCAAATAAGCCCAATGAAA GAGGAAAAGAGGAGACACTGAGATTTGGCCCAAACTAACGCATATATTTGTGTTTTACATTTATACCCCTATGACCGACA TCAATTTACCGACAGACTCTACTTTCGAGAATGGCGGGAACGTGAACTGAACCCTAATCACCTTTTTTCTCGATTCCCCA CCAATGGCTATATGATTACAGTAGCCCCCTTCGTGTCTCTTCGTTTCCAGTTCCCTCTTTATATAATAAATCGCTCCACT CTCTTTTTCCGAGAAAGAACACAGTATCTCGTCTACAACACCTGTCACATTTTGAGAACAACGGCGAAAACAATGCCGGT GGTTGCTGAGTACGCGAAGTCGAATCGGTCGTCGTGTAGGTCATGTTCGAACAAGATAGCTGTGAAATCGCTTAGGTTAG GGTTGATTAGCAAAGGACGTGGCGGGGTTGACATGACCAGGTGGCACCATTTCGATTGTTTTCCGACTGATTCTGAGTCG ATTGCTTCCGTTGATGATATTCAAGGCTTATCCGCTTTAGAGAAAGAAGATCAAGATGCTTTGACGAAATTGGTGGAACA ATGTGGTAAAGTACCTGCAAAAAAACAGCCTGATGAGAAGAAGGGGAAGGCTAAAAAGCATATAATGGGGCCGAAGGGGC TTACGAAGGCGGCAACATCTTCAAAAGTTATTGCTGATAATGCGAAATCGAGCAGATCATCATGCAACAGGTGTTCTCAG ACAATCGTTTCAAAGGATCTGAGAGTGGGGCTGGTTACTGAAGACTCCAGGGGATTTGATATAACAAGATGGCATCACTT GGGTTGTTTTCCCATTGATTTTCATCCAATTGATTCTGTGGAGGACATTGGTGGATATTCATCGCTTGAGAAAGGTGACC AGATGGAATTAAAGTATTTGGCCGAAGATGTCCAGAAGATGGATGAAGGTGATGATGAAGCTATAGCTGATAATGAGCTA ACGGAGGAGACCAAAAAAGGAAAACATTCTCCTGTTGCAAAATTGGTGGAACAACCTGGCGAGCCTGCAAAAGAAGATGA GGATGAAGAGAGTAAAAAGCCTGCATCAGATGAAATAAGTGAGCAGAAGACTAAGGATGTGAAAAATTCTCCTGATTCTT CAAAAGTTATTTCTGAGTATGCGAAATCGAGCAGATCAACATGCAAGAAGTGCTCTCAGACTATCGCTGCAAAGGAACTG AGGTTGGGGCTAGTGACCAGAAACTTCCGTGGCTTTGATATGAAACAATGGCATCACTTGGGTTGTTTTCCTGTCGACTC TGATCCAATTGTTTCGGTAGAGGACATTGGTGGATTTTCAGAACTGCAGAGTGGTGATCAGGATGCATTAAAGGAATTGG TCCAACAATGCGGGAAGCAGACTTTGGTAGATAAGATGGATGAAGATAATGATGACACTGAAGCTAAAATTAAGCTAACT GAGGAGACAAACAAAAGAAAACATTCTGAGGTTGGGGAGATGGTGGAAGAAGATGAATCGCTAACTAAAGCGAAACAACA GATGGCTAAAACACATAAGGTGAACATGAGTGAGTCAACTTCTCAGGTCGAGGTTGAAGCAGAAATCACTCTTTCAGCGT CTGATGTGAAGGATAAGTACAGGGATGCCAATCTATTACCAAAATGGAAAGCTTTTGAGACAGTTATATTCCTTGAGCGG GATGATGGTCTTAATGATTCAGAAAAAATAGCTGCATTTGACTTTGATGGATGCCTTGCAAAAACATCTGTGAAAATAGT AGGGGCAGATGCATGGTCCCTAATGTATCCTTCCATTCCTGAGAAACTGCAAAGTCTGCACGATCAAGGCTATAAGCTGG TCATTTTCACAAATGAATCCAACATTGATCGATGGAAGAACAAGCGGCAAGCTGCTGTAGATTCGAAAATTGGACGGCTC AACAGTTTTATCGAGCGCGTGAAGGTCCCCATTCAGGTGTTTATAGCCTGTGGAGTCTCAAGTTCTGGTGGTAAAGGTGG TAAAGATGACCTTTACCGCAAACCCAAGGCTGGAATGTGGCAACTCATGAAGAAGCATTTTAACTCTGGAATTGCAATTG ATATGGATAAATCCTTCTACGTTGGGGATGCAGCTGGAAGAAAAATGGATCACAGCGACGCTGACATCAAATTTGCACAG GCGAGTGGGCTAAAGTTTTTTACCCCGGAGGAATACTTTATCCCCTCAAGTACATCGCCAGGGACTTAGCTGTATCAGTC TTGTGCTTGTGTTTGCAGTTTGCAACTTCTTTTGCTTTTTTTTTTGTGATGAAACTAGTAGCTAGCTTTTGTGATAAAAC TAGTAGTG ..........................................................................N..... ......................................................................N......... ..............................i................................................. ...N.......N.................................................................... ........................................................................i....... ..........................................N..................................... ................................i............................................... .............N.........................................N........................ i...............................N...............................i............... .......................................N.....................N.................. ....................................................................N........... ................................................................................ ..i........................N..........i...N........N..N.................N....... ............................................................................N... ..N..........................N..........................N....................... ..................N.....................i....................................... .......................................i.......N................................ ..............................................................N................. .......N...........................i...N........N..N............................ .......................................i............N........................... ...N....................N..................N.................................... ........i...N.........N......................................................... ............N..................i................................................ ................................................................................ .....N............................i...........i................................. ..i........................N................i................................... ................................................................................ ................................................N............................... ........ Pos Score Pred ------------------------ 75 0.498 - 151 0.286 - 191 0.578 Yes 244 0.487 - 252 0.367 - 393 0.757 Yes 443 0.200 - 513 0.696 Yes 574 0.116 - 616 0.378 - 641 0.664 Yes 673 0.083 - 705 0.764 Yes 760 0.192 - 782 0.371 - 869 0.429 - 963 0.879 Yes 988 0.108 - 999 0.688 Yes 1003 0.232 - 1012 0.315 - 1015 0.177 - 1033 0.206 - 1117 0.081 - 1123 0.142 - 1150 0.144 - 1177 0.294 - 1219 0.137 - 1241 0.656 Yes 1320 0.563 Yes 1328 0.453 - 1423 0.142 - 1448 0.350 - 1476 0.849 Yes 1480 0.169 - 1489 0.218 - 1492 0.306 - 1560 0.788 Yes 1573 0.151 - 1602 0.677 Yes 1626 0.790 Yes 1684 0.081 - 1705 0.110 - 1724 0.207 - 1762 0.135 - 1765 0.227 - 1774 0.115 - 1807 0.228 - 1811 0.691 Yes 1849 0.679 Yes 1853 0.369 - 1863 0.309 - 1933 0.194 - 1952 0.661 Yes 2086 0.335 - 2115 0.719 Yes 2127 0.686 Yes 2163 0.671 Yes 2188 0.340 - 2205 0.751 Yes 2369 0.040 -

Explain the output -- Processing locus 128: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAAATGTAAAGAGTTACAAGTTTCCCTTTTTCTTTCTTTTTTAGGCTATAAATTTAACATAGTATCTTATTTCTTTCT AAATCAGGACAAAAAGTTTGATTTTTTTCTTTTCTTTTCTTTTTCCCTGTGTGTGGTTTCATGGGATATAGGGATGAAGA AACAATGGCTACCGGACAAAACAGAACAACTGTGCCAGAGAATCTGAAGAAACACCTCGCAGTTTCAGTTCGAAACATTC AATGGAGTTATGGTATCTTTTGGTCTGTCTCTGCTTCTCAGTCTGGAGTTTTAGAATGGGGAGATGGATACTATAATGGA GATATCAAAACGAGGAAGACGATTCAAGCTTCGGAGATCAAAGCTGATCAGCTTGGTCTACGGAGGAGCGAGCAGCTTAG CGAGCTTTACGAGTCTCTCTCCGTCGCTGAATCTTCTTCTTCAGGCGTTGCTGCCGGATCTCAAGTCACCAGACGAGCTT CCGCCGCCGCACTTTCACCGGAAGATCTCGCCGACACCGAGTGGTACTATTTGGTTTGTATGTCTTTCGTCTTCAACATT GGTGAAGGAATGCCTGGACGGACGTTTGCAAACGGTGAACCGATATGGTTGTGCAACGCTCATACGGCGGATAGTAAAGT GTTTAGCCGTTCTCTTCTAGCAAAAAGTGCTGCGGTTAAGACAGTGGTTTGCTTCCCGTTCCTTGGAGGAGTCGTTGAGA TTGGTACCACAGAACATATTACGGAAGACATGAATGTAATACAATGCGTGAAGACATCATTCCTCGAAGCCCCTGATCCG TACGCTACAATATTACCAGCAAGATCCGATTATCACATCGACAACGTTCTTGATCCGCAACAGATTCTAGGCGACGAGAT TTACGCGCCTATGTTCAGTACGGAGCCTTTTCCAACAGCTTCTCCGAGCAGAACTACCAACGGTTTCGATCAAGAACATG AACAAGTAGCAGATGATCATGATTCTTTCATGACCGAAAGAATCACTGGAGGAGCTTCTCAGGTGCAAAGCTGGCAGCTC ATGGACGACGAGCTTAGTAACTGCGTTCACCAGTCGCTAAATTCCAGCGATTGCGTCTCTCAAACGTTTGTTGAAGGGGC GGCTGGACGGGTTGCTTACGGTGCAAGAAAGAGTAGAGTTCAAAGACTAGGGCAAATTCAAGAGCAACAGAGAAATGTGA AGACATTGTCATTTGATCCAAGAAACGACGACGTTCATTACCAAAGTGTGATCTCAACGATTTTTAAGACCAACCATCAG TTAATTCTCGGACCGCAGTTTCGAAACTGCGATAAACAGTCAAGCTTCACTAGGTGGAAGAAATCATCGTCATCATCATC AGGAACCGCCACGGTCACGGCACCATCACAAGGAATGTTAAAGAAAATTATTTTCGATGTTCCGCGAGTGCACCAGAAAG AGAAGTTAATGTTGGACTCACCAGAAGCCAGAGATGAAACTGGGAACCATGCGGTTTTAGAGAAGAAGCGCCGCGAGAAA TTGAACGAACGGTTCATGACCTTGAGAAAAATCATTCCGTCAATCAACAAGATCGATAAAGTATCGATTCTTGACGATAC GATAGAGTATCTTCAAGAACTCGAGAGACGGGTTCAAGAACTAGAATCTTGCAGAGAATCAACCGATACAGAGACTCGTG GGACGATGACGATGAAGAGGAAGAAACCATGCGACGCAGGAGAAAGAACATCAGCTAATTGCGCAAATAATGAAACAGGA AATGGGAAGAAGGTGTCGGTTAACAATGTTGGTGAAGCCGAGCCAGCAGATACCGGTTTTACTGGTTTAACCGATAATTT AAGGATCGGTTCGTTTGGTAATGAGGTGGTTATTGAGCTTAGATGTGCTTGGAGAGAAGGAGTATTGCTTGAGATAATGG ATGTGATTAGTGATCTCCATTTGGATTCTCATTCGGTTCAATCCTCGACCGGAGACGGTTTGCTCTGCTTAACCGTCAAT TGCAAGCACAAGGGGTCAAAAATAGCGACACCAGGAATGATCAAAGAAGCACTTCAAAGGGTTGCATGGATCTGTTGAAG ACTACTTAGTTAAAATTGACAGCAAAGAAAAAACATTCCCGGTTTGGTTTCTATTCTTTGGTTTTCTTCTAACCGGGTTT TAGGAATTAATGTTATG .....N.......................................................................... ............................................................N............N...... ....i........................................................................... ................................................................................ ................................................................................ ...........................................................i.................... .........i..................................N................................... ................................................................................ .............................i...N.........i.................................... ................................................................................ ..........i..................................................................N.. ............N.....N..........i.................................................. i............................................................................... ..........................................................................N..... ................................................................................ ................................................................................ ..................................i.....................i....................... ........N........................N..............N............................... ...............N................................................................ ................................................................................ .....i.....N................N........................................N.......... ....................N.....................N.................................i... N............................................................................... ....................................N............................N.............. ................................................................................ .........N....N.. Pos Score Pred ------------------------ 6 0.155 - 141 0.362 - 154 0.263 - 165 0.783 Yes 242 0.487 - 250 0.459 - 296 0.677 Yes 304 0.732 Yes 316 0.474 - 540 0.625 Yes 570 0.756 Yes 605 0.440 - 750 0.652 Yes 754 0.365 - 764 0.739 Yes 891 0.503 Yes 958 0.372 - 973 0.175 - 979 0.419 - 990 0.741 Yes 1041 0.712 Yes 1195 0.388 - 1395 0.733 Yes 1417 0.559 Yes 1449 0.251 - 1474 0.243 - 1489 0.314 - 1536 0.403 - 1686 0.754 Yes 1692 0.398 - 1709 0.461 - 1750 0.197 - 1762 0.307 - 1786 0.195 - 1861 0.143 - 1883 0.227 - 1917 0.620 Yes 1921 0.321 - 2037 0.149 - 2066 0.278 - 2170 0.058 - 2175 0.040 -

Explain the output -- Processing locus 129: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGCTAGGATGATATTGGTTTGCTTGTATGGTCTCTGACGTTAATTCCCATTAGTTCTTCTTATTTGGTTTGCTTGCTTG TCTTTTTATCCAGTGTTTTAAGTGGAAAATATGCTTATGTGGTCATTTTCTTACTATTTTTCTGCAGATTCGGAAGGAAA ATTCATGAATGATTCTCACGGGAAGAAAAAGCAGGTACATAACTCGGACAATGAACCTTCAAGCGAAATATTGAGTAGAT CTAATACTTGTGAAAGCTTTCCAGATCACGGGAAATCAGTTGTCACTGTGCCAGACTCAGAGGATGTACAAGATGGTCAT ATGCCAGCTGCGAGTTCAAGTTTCCCTGAAGCTGCACGAGCGTTTGTTGATGCAATCAGGAGAAACAGAGCATATCAGAA ATTTCTTAGAGGAAAATTGGCAGAAATTGAAGCAACGATCGAGCAGAACGAGAAACACAAGAAAAATGTTAGAATAGTCA AAGATTTTCAAGCTTCTTGCAAGAGAATTACTAAACTGGCACTCTGTCAGAGGAAAGATCCTCGTGTCGAATTAATCTCA ACACGGAAATCTGGGCCTTGTGATAGCTCCGAGGTAATTGGGCCTTGTGATAGCTTCGAGGGTAATGATAAAAAGATATC TCCTTTGACGTTGGGTCCAGCGGAAAACCCTTGTGTTGAAAATTATAGGATGGCACTAGAGAAATATCCAATTTCAGTGA AACGCAGAAAATGGTCTACTGAGGAGAACAAAAACCTCGCAAAAGGTTTAAAACAAGAAGTTCAAAAGATACTGCTTTCT GAAGCAATTGAACGGTCCAGTGACTTGGAAGGAGCTACATATGATATAGACACTATCAATGAATCGATCGGAAATCTTGA GATCACACCAGAAATGATTAGGCAGTTTCTTCCGAAAATTAACTGGGATTCGTTGGATATCAAGGACCGTTCTGCTGCAG AATGTGAAGCTCGGTGGATGAGCTCGGAAGATCCATTGATTAACCACGGTCCGTGGACTGCAGCAGAGGACAAAAACCTA CTCCGAACTATCGAACAAACGAGTCTTACAGACTGGGTTGATATCGCGGTGTCACTAGGGACAAATAGGACCCCATTTCA ATGTTTGGCTCGATATCAGAGAAGCTTGAATCCAAGCATATTGAAAAAAGAATGGACTGCAGAGGAAGATGACCAACTTC GTACTGCAGTAGAGTTATTTGGGGAGAAAGACTGGCAATCTGTTGCAAATGTATTAAAAGGAAGAACTGGAACTCAGTGC TCGAATAGATGGAAAAAGTCGCTTCGCCCTACCAGAAAAGGGACATGGAGCTTAGAGGAAGACAAACGTGTAAAGGTAGC TGTGACACTCTTTGGATCTCAAAACTGGCATAAAATTTCTCAGTTTGTTCCTGGACGGACTCAGACTCAGTGTCGGGAGA GATGGTTAAATTGTTTAGATCCCAAAGTAAATCGTGGGAAGTGGACTGAGGAAGAGGATGAAAAGTTGAGAGAAGCAATT GCAGAGCATGGATATAGCTGGTCTAAGGTGGCTACTAATCTGTCATGTCGTACTGACAATCAGTGCTTGAGGAGATGGAA GAGATTATATCCTCATCAAGTGGCTCTTTTACAAGAAGCTAGAAGGTTACAAAAAGAAGCTAGTGTAGGTAATTTTGTTG ATCGGGAATCAGAGCGTCCTGCTCTTGTCACAAGCCCTATTTTGGCACTACCGGATATATCTCTAGAGCCAGAACCCGAT AGTGTAGCCCTGAAGAAGAAACGCAAAGCCAAGCAGAAGAAGTCAGATGCAGAGAGACAACCAAAGAGACGGAGAAAAGG ATTAAAGAACTGCTCAGGAGATGTATGTAGACAAGAGAATGAGACTGTTTGTGAAAATGAACCGAACAATGGAGGAGAGG AAAGGATGTTGGCATTGGAATGTCATGACGAGATCCAAGACAATGCAAAGGAGAAGCCAAAACAAAGACGCAAAAGTGTT GCAGAAACTGTTTGTGAAAATGAACCAAACAATGGAGGAGAGGAGAGGATGTTGGCATTGGAATGTGATAACGAGATCCA AGACAATGCAAAGGAGAAACGAAAACGAAGACGCAAAAGTGTTGCAGAAACATCTAACAACACCACTGGTTTAAAAAAAC TTACGCCTAGGAGAAGGAAAATATCTGCAGTTGTTCCTATTAAGAACCAAGATGCACCAAATTAGTTGTTTTTTTGGCTC TTGAAAATTTTGAAATTTATTGTATGTATAAATATGTGTAATCCAAACATCTTTAGAATAGTCACTGATTCTAACAACAA CGAC N.......N..................N.................................................... ..............................i.....i........................................... ....i...N.........................................N............................. ...............................................................N........N....... i................................................N.............................. .................................................................N.............. ................................................................................ ................................................................N............... .................................................i.............................. ..........i..................................................................... ........................................N.................N..................... .............i.................................................................. ................................................................................ N..................................................i................N........... ................................................N............................... ........N...................................i................................... ................................................................................ .......N....................................N.............................N..... ................................................................................ ................................................................................ ..............................................N................................. ....................N...N.............N.................N...........i........... .....i.............N....N.................N..................................... ...................N...........i................i.............N................. .....N.......................................................................... ...................................................N............................ .......................N.........N.............................................. .... Pos Score Pred ------------------------ 1 0.164 - 9 0.223 - 28 0.154 - 111 0.561 Yes 117 0.695 Yes 165 0.606 Yes 169 0.432 - 211 0.418 - 304 0.194 - 313 0.458 - 321 0.708 Yes 370 0.136 - 466 0.376 - 625 0.151 - 690 0.817 Yes 731 0.569 Yes 841 0.161 - 859 0.182 - 894 0.657 Yes 962 0.601 Yes 978 0.521 Yes 1121 0.190 - 1172 0.513 Yes 1189 0.209 - 1249 0.390 - 1289 0.407 - 1325 0.507 Yes 1442 0.421 - 1498 0.126 - 1528 0.215 - 1565 0.243 - 1595 0.175 - 1807 0.319 - 1861 0.496 - 1865 0.143 - 1879 0.382 - 1897 0.274 - 1909 0.509 Yes 1926 0.685 Yes 1940 0.209 - 1945 0.465 - 1963 0.310 - 2020 0.307 - 2032 0.656 Yes 2049 0.608 Yes 2063 0.166 - 2086 0.359 - 2212 0.233 - 2264 0.043 - 2274 0.109 -

Explain the output -- Processing locus 130: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAATAATGGCATAAAGCAGACGCATTAACTTTTGTAATTGGGGTTTTACCTTTCGTTTTCACTTTAATTATTTTAACTAA TCCTTTTTACAAGACCCATAAAGAACAAAAGTCAAAATAATCGATAGTCTCAAAAAGTCAAGAATCTCTCACACACTCAT TATGAACACGCACCACCAAACAACACTTCCATTGATCCTCATAATAATCTCCACCAATACTCATTTCCCTCTTGGATTCT TCAAACTCCTCTCTGAAAGTTCTCATTTTTGGTTTACATTTCCTTTGAGTCCACTGTTACTTACTAAAATGGTGAGCAAA TGGAAGAAGATGAAGCTAGCTTTGAGTTTGAATCTCTGCAACTATCTCCCCAGAACCCTCGAGGAGGAACCACCATCATT GGCTTTGAATTCCGCTGAGACATTATCAGATGCTGCTTTGCTCTCTCCTCTTAACTGGCCCATGACTCCAACACCTTCTT CTTACCGTCGAAGGTTGTCCAGAAGTAGCAGCAAATCGTCAAAGACTTGCTCTATATGCTTGAACAAGATGAAAGAAGGT TGTGGACATGCAATTTTCACAGCGGAGTGCTCGCACATGTTTCATTTTCATTGCATTGCTTCGAATGTGAAACATGGCAA CCAAGTTTGCCCAGTGTGCCGAGCTAAATGGAAGGAAATCCCTATACAGAAGCCGAGTTTGGATCTTCCTTATTACCCCT TTGACAGGTGCAATAATGATGCAGCCATTAGCCTTTTCCGCTGCTTACCTCCTTCTCAACGAGCTATAACCCAAGGGCAT CCCGAGCCAGCTACTTTCGATGATGATGAACGGTTAGAAGAGCAGATTGTTTTTGATGGTGAAACTGAAGTCTTGAAGAA GGAGAACCGTGATTATGTGCGAATGATGGACATGAAAGTGTATCCAGAGGTCTCAGCTGTACCACAATCCAAGTCTTGTG AGAATTTTGATGTACTAGTGCATCTCAAAGCAGTTACTGGTGATCAGATTTCTCAGTATCGCCGAGCGCCAATTGATTTA GTTACAGTGCTAGACATCAGTGGTAGTATGGGAGGAACTAAGCTAGCACTTCTGAAACGAGCAATGGGTTTTGTGATTCA AAATCTTGGGTCCAGTGATAGGCTCTCAGTGATTGCTTTCTCTTCAACAGCTCGACGTCTCTTCCCTTTAACCCGAATGT CTGATGCTGGAAGACAGCTAGCCCTTCAAGCTGTTAACTCACTGGTTGCAAATGGTGGGACCAATATTGTCGATGGTCTT AGAAAAGGTGCCAAGGTTATGGAAGATAGGCTAGAAAGAAATTCTGTTGCCAGCATTATCCTTTTGTCTGACGGGCGAGA TACATATACTACGAACCATCCTGATCCAAGTTACAAGGATGCTCTTGCACAGTGCATTGGTGGACTTTTAAGCGTTGCAG TGCAGGAGCTACGAGTAGAAATTGAAGGTGTGAGCCCAAATGTTCGACTTAGCTCGATAAAAGCTGGGAGTTACTCTAGC CTTGTAACTGGTGATGGCCACTCAGGGTTGGTTGATCTTGGTGATCTTTATGCTGATGAAGAGAGGGATTTCTTGGTTTC TATCAACATCCCTGTTGAGGAAGATGGACATACACCATTGCTAAAGCTGAGATGTCTCTACATTAATCCCTTGACAAAGG AGATTACAACGCTTGAAAGTCACGTGCTTCAGATTCGAAGACCAGAATATGTTGCTGAAGAAAAAGTTGTTCCCATCGAA GTGGTCAGGCAAAGAAACAGATTCCTAGCTGCAGAGGCAATGGCCCAAGCAAGAACTTTAGCAGAGCATGGAGATTTAGA GGCAGCCGTTAAAGCCATTGAGAATTTTAGATTGGTTTTGGCAGAGACGGTTGCAGCAAAATCATGTGACAGGTTTTGTG TTGCTTTGGACTCGGAACTGAAGGAAATGCAAGGTAGAATGAGGAGTAGTCACATGTACGAGGCATCGGGAAGAGCTTAT ATTCTTTCTGGACTTAGCTCACACTCATGGCAAAGAGCAACAGCGAGGGGAAATTCAAGGGACAGTTCGAGCTTTGTCCA GGCTTATCAGACTCCATCAATGGCTGAGATGCTGCGTCGATCACAGGCCATGTTTCTAGTTAGTCCATCACATCATAGAC TCATTCAGCCCTTGCTCTCTTTTGCTTCACAACCAAAGCCAAGGTAAAGAACAAATTTTGACATCAATCCAATGCTTATG TACAAGTAGTTACGTAGCAAGCTTAAACTTGTTTTGTCTGTAAAGAAACAACCACAAGCAGTCGAA .....i.......................................................................... ................................................................................ ....................................................................i..........i .........i...................................................................... .............................i...............................N.................. .......................................................N............N........... .......N............................i...........................N........N...... ...........................N.................................................... ...............N..i............................................................. ...................N..N..N.............................N........................ ..............N.......N..i.....i................................................ .........N...................................................................... ...........................N...................................i................ ............................................................................i... ...N...............................................N....................N....... ..................i............................................................. ......................................N......................................... .......................................N........................................ .............N...................................N.....N........................ .......................N...........................N............................ ................................................i............................... .......................................i...........................N............ ...............................................................i................ ..........................i...........N..............N.......................... ..........................i..................................................... ...................N........N....................N.............................. .......................................................................N.....N.. .................................................................. Pos Score Pred ------------------------ 6 0.633 Yes 162 0.407 - 309 0.706 Yes 320 0.787 Yes 330 0.528 Yes 430 0.634 Yes 462 0.333 - 536 0.290 - 549 0.385 - 568 0.407 - 597 0.585 Yes 625 0.415 - 634 0.432 - 668 0.377 - 736 0.495 - 739 0.701 Yes 820 0.087 - 823 0.090 - 826 0.200 - 856 0.131 - 895 0.195 - 903 0.379 - 906 0.725 Yes 912 0.586 Yes 970 0.180 - 1068 0.387 - 1104 0.773 Yes 1197 0.805 Yes 1204 0.245 - 1252 0.408 - 1273 0.173 - 1299 0.500 Yes 1399 0.183 - 1480 0.155 - 1534 0.259 - 1570 0.125 - 1576 0.110 - 1624 0.178 - 1652 0.311 - 1729 0.549 Yes 1800 0.813 Yes 1828 0.099 - 1904 0.585 Yes 1947 0.747 Yes 1959 0.220 - 1974 0.423 - 2027 0.673 Yes 2100 0.443 - 2109 0.318 - 2130 0.264 - 2232 0.097 - 2238 0.064 -

Explain the output -- Processing locus 131: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCTATTCTCTAAAATTTTGATATTAAATTTGTACAAAATACATTTCTACGTGGATTTAAGGTGTTAAAAGAGTTCGATTA GTAAGCTTTACATTTTATTGATACTACCACTATATATACGTTGGAATGTCATATCAAACACAGATTAACAAATTCTCTCA AATGGCTCTCTCCGTATTTGCCTTTCCTTCTTACATAAATAGGAATCCTTCACTAAAATATCTTAAACCTTCTTCTATGT CTTCTACAAAATATTCAAAAGTAAGAGCAACAACATTTTCAGAGAAAGGTGAATATTATTCAAACAGACCACCAACTCCT TTATTGGACACAATCAACCATCCAATGCACATGAAAAATCTCTCCATCAAAGAACTCAAAGTTCTTTCGGACGAGTTGAG ATCTGATGTTATTTTTAATGTTTCGAAAACTGGAGGACACTTGGGTTCGAATCTTGGTGTTGTTGAGCTCACCGTGGCCC TTCATTACATCTTCAATACTCCTCATGATAAGATCCTTTGGGATGTTGGTCATCAGTCTTATCCTCACAAGATTCTAACG GGAAGAAGAGGAAAGATGAAGACAATAAGGCAGACCAATGGCCTCTCCGGCTACACCAAGCGAAGAGAGAGTGAGCATGA CTCTTTTGGCACCGGGCACAGTTCGACCACACTATCTGCAGGCTTAGGGATGGCTGTAGGGAGGGATTTGAAGGGGATGA ACAACAGCGTGGTTTCGGTTATAGGCGATGGTGCTATGACAGCTGGACAAGCTTATGAAGCAATGAACAATGCTGGCTAC TTACACTCCAACATGATTGTGATTCTCAACGACAACAAACAAGTATCTTTGCCTACTGCTAACTTGGATGGACCAACTCA ACCTGTTGGAGCTCTGAGCTGTGCTCTTAGTAGGCTGCAATCTAATTGTGGAATGATTAGAGAGACTAGTTCAACACTGT TTGAAGAACTTGGTTTTCACTATGTTGGTCCAGTTGATGGACACAACATAGATGATCTGGTCTCCATTCTTGAAACATTA AAGAGCACCAAAACCATAGGACCGGTTCTTATCCATGTCGTGACTGAGAAAGGTCGTGGATATCCTTACGCAGAGAGAGC TGATGACAAGTATCATGTTTTAAAATTTGATCCAGAAACAGGTAAACAGTTCAAAAATATTTCCAAGACTCAGTCTTACA CTTCCTGTTTTGTGGAGGCCTTGATTGCAGAAGCAGAGGCAGACAAAGATATTGTTGCCATTCATGCAGCCATGGGAGGT GGAACCATGTTGAATCTCTTCGAAAGCCGCTTTCCTACAAGGTGTTTCGATGTCGGCATAGCAGAACAACATGCAGTTAC CTTCGCTGCTGGTCTTGCTTGCGAAGGACTTAAGCCCTTTTGTACAATCTACTCATCTTTCATGCAACGGGCATATGATC AAGTTGTACATGATGTTGATCTACAGAAACTGCCTGTGAGATTTGCAATAGATAGAGCAGGACTTATGGGAGCAGATGGT CCAACACATTGTGGAGCATTTGATGTGACGTTTATGGCATGTCTACCAAACATGATAGTAATGGCTCCATCTGATGAAGC AGAGCTTTTTAACATGGTTGCAACCGCTGCAGCTATTGATGACCGTCCTTCTTGCTTTCGATATCATAGAGGAAATGGTA TTGGTGTTTCACTTCCTCCTGGTAACAAAGGTGTCCCTCTTCAGATTGGGAGAGGTAGGATACTAAGGGACGGCGAGAGG GTTGCGCTTTTGGGCTATGGATCAGCGGTGCAAAGATGTTTAGAGGCTGCATCTATGCTAAGCGAACGCGGATTAAAGAT AACAGTAGCGGATGCAAGATTCTGTAAGCCGTTAGATGTTGCTCTCATTCGTAGCTTAGCTAAATCACACGAGGTTTTGA TCACGGTTGAAGAAGGTTCCATTGGAGGATTTGGATCGCATGTGGTACAATTTCTTGCACTTGATGGCCTTCTTGATGGA AAGCTCAAGGTATATCGAACATGGATCACCAATGGATCAACTAGCTGAAGCTGGCCTCACAGCGTCTCACATTGCAGCCA CTGCACTTAACTTAATGGGAACACCTAGAGAAGCATTGTTTTGGGAGTAACGGGATCTCCATGAGAG ................................................................................ .............................................N.................................. ................................................................................ ........................N.....N................................................. .....N...........N.............................................................. ........................N.................i..................................... ...............i.....................i......................................N... .................................................i..........................i... ...........................N.......i..................N.......i......N.......... ............i......................................................N............ ....................................................i........................... .....................N..............N..............N............................ ..................................N............................................. ..N...........N................................................................. ...............................................................N.......i........ ......i..........................................i....................N......... .............................................................i............N..... .........N..N....................................................N.........N.... ......................N..........i....N............N........i............N...... .............i........................N...................................N..... ................................................................................ ................N..................i..................N......................... ...........N.......................N............................................ .......................................N.......................i...........N.... ....................N..........i................................................ ..............N.............................................N...... Pos Score Pred ------------------------ 126 0.473 - 162 0.669 Yes 237 0.626 Yes 345 0.303 - 351 0.312 - 406 0.073 - 418 0.095 - 505 0.201 - 523 0.576 Yes 576 0.750 Yes 598 0.656 Yes 637 0.454 - 690 0.871 Yes 717 0.597 Yes 748 0.146 - 756 0.547 Yes 775 0.215 - 783 0.609 Yes 790 0.299 - 813 0.607 Yes 868 0.455 - 933 0.795 Yes 982 0.316 - 997 0.151 - 1012 0.184 - 1075 0.491 - 1123 0.158 - 1135 0.202 - 1264 0.110 - 1272 0.616 Yes 1287 0.835 Yes 1330 0.600 Yes 1351 0.483 - 1422 0.645 Yes 1435 0.384 - 1450 0.213 - 1453 0.285 - 1506 0.429 - 1516 0.089 - 1543 0.073 - 1554 0.616 Yes 1559 0.377 - 1572 0.486 - 1581 0.659 Yes 1594 0.114 - 1614 0.838 Yes 1639 0.445 - 1675 0.499 - 1777 0.163 - 1796 0.544 Yes 1815 0.377 - 1852 0.390 - 1876 0.180 - 1960 0.183 - 1984 0.625 Yes 1996 0.442 - 2021 0.312 - 2032 0.557 Yes 2095 0.058 - 2141 0.050 -

Explain the output -- Processing locus 132: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGATAAGCCAAGTTACCTCTTTTTTTTTATACATATGTTTTCAAAAGTTCCTAAAAAATTGTTTGGTTTTGTTTTATTT TTCTCTTCTTAAGGTACTTAAGTTTGTGATTTGATGATGACTGGTACTACTTTGATATTGGAACCAGTTATGGATTCCAA AGATGAGCTAGTGAAACAACATGCCAAAGTTGCAGAAGATGCTGTTGCTGGGTGGGAGAAAGCTGAGAATGAAGTGGTTG AGCTGAAGCAAAAGCTCGAGGATGCGGCTGATAAGAACATTGTCTTGGAAGATAGGGTAAGTCATCTTGATGGGGCGCTT AAGGAGTGTGTGAGACAACTAAGGCAGTTTAGAGATGAACAAGAGAAGAATATTCAAGCAGCTGTGACTGAGAGCACCAA GGAGTTGCATTCTGCTAACACTGGTCTAGAGAAGCGAGTTCTTGAGCTCCAGAAAGAAGCAGAAGCTGCTAAATCTGAAA ACATGATGCTGAGACGCGAGTTTCTTACACAGCGTGAAGACCTTGAGATTGTGATGATTGAGAGGGACTTGAGCACTCAA GCAGCAGAAACTGCTAGTAAGCAGCATTTAGACATCATAAAGAAGTTGGCGAAACTCGAAGCTGAGTGCAGGAAGCTTAG GATTTTGGCTAAAACATCATCATCGCTATCATCTAATCAATCTGTGGATAGTCATTCAGATGGAGGAAGGGAGAGAGTTG AGGGGAGTTGCTCTGATTCATGGGCATCATCAGCATTTATTTCTGAGTTAGATCAAATCAAGAATGAAAAAGGCGGCAAT AGAAGTCTTCAGGGCACTACTTCATCAACTGAAATTGATCTCATGGATGATTTCCTTGAGATGGAACGTCTTGTGGCTCT TCCTACTGAGACACAGGCTAAAAACTCCAAGGATGGATATGAATTGAGCTTAATGGAGAAGTTGGAGAAGATACAAGCAG AAAAGGATGATCTTGAAAGAGAAGTTAAATGTTGTAGAGAAGCGGAAAAGAGATTGAGCTTAGAGATTGAAGCAGTTGTT GGTGACAAAATGGAGTTGGAAGATATGTTAAAGAGGGTGGAAGCTGAGAAAGCTGAGCTAAAGACATCTTTTGACGTGCT CAAAGATAAATATCAAGAGTCAAGAGTTTGTTTTCAAGAAGTTGACACAAAGTTGGAGAAGCTACAAGCAGAAAAGGATG AGCTTGACAGTGAAGTTATTTGTTGTAAAGAAGCAGAGAAGAGATTCAGCTTAGAACTCGAAGCTGTAGTTGGTGACAAA ATTGAGATGGAAGATGAGTTGGAGAAGATGGAAGCTGAGAAAGCTGAGCTAAAGATATCTTTTGACGTAATTAAAGATCA ATATCAAGAATCTAGAGTTTGTTTTCAAGAAGTTGAGATGAAGTTGGAAGCGATGAAAAGGGAGCTTAAACTAGCTAATG AATCGAAAACACAAGCCGAATCTCGGGTGACCAGAATGGAAGCAGAGGTGAGAAAGGAGAGGATTGTCTCTGATGGGCTA AAGGAAAAGTGTGAGACATTTGAAGAAGAGCTTAGAAGAGAGATAGAAGAGAAGACAATGATCAAGAGAGAAAAAGTGGA ACCAAAGATCAAACAGGAAGACATAGCAACAGCTGCAGGAAAATTTGCAGATTGTCAGAAAACAATAGCATCACTTGGGA AACAGCTACAATCTCTTGCAACACTAGAAGAATTCTTGATCGATACAGCTAGCATTCCAGGTTCTGCAAGGTCAGTTCAC AACAAGGAAGCTTTGTTAGGAAAAGATCCTCATGAGTGCATCAAAACAATCAATGGAAGATCACTTGAGTTTCTTGCAAT CCAGAACAGCAATAACAAGACCTCACCTCCTTGTTCATCTTCTTCGGACTCAACAACAGTGTCATTAATTATGTCATCAA ACCGAGGGAGTTCTGAGAAGAATCGCAACGGATTTGCCACGGTTTTCACTCGAAGTAGAAACTCAGTAAATTTGGGGATT TAGGAGAAGCAATAGGTTCGTTCTTGGAGTATAGCTAGAATCAATCTGTTGTTAAGCTGGATTTCTTACCATAATTTTCA GATTCTGACTTGTTAGTAGCAA ...................................N............................................ .................................i..i................................i.......... ..N.................N.................N.............................N........... .....................N...............................................N.......... ..................................N............................................. ................................................................................ ..i..N...............................................N.......................... ................................................................................ ...........................................................N.................... ...................N...........................................N................ ..........................................i...N.............i................... ................................i.....N.............i........................... ......N.....................N................................................... .........i..............N....................................................... .............................................................................N.. ................................................................................ ......i......N.............N.................................................... .....................................i..............i........................N.. ...................................i....................................N....... .........................................................i...................... ................................................................................ ................................................................................ ...............................N....................i........................... ......................................................................i......... ................................................................................ ................................................................................ ...................... Pos Score Pred ------------------------ 36 0.272 - 114 0.548 Yes 117 0.866 Yes 150 0.846 Yes 163 0.404 - 181 0.148 - 199 0.224 - 229 0.114 - 262 0.198 - 310 0.080 - 355 0.311 - 483 0.840 Yes 486 0.373 - 534 0.486 - 700 0.413 - 740 0.217 - 784 0.095 - 843 0.665 Yes 847 0.152 - 861 0.546 Yes 913 0.533 Yes 919 0.307 - 933 0.687 Yes 967 0.296 - 989 0.492 - 1050 0.577 Yes 1065 0.320 - 1198 0.328 - 1287 0.572 Yes 1294 0.247 - 1308 0.484 - 1398 0.829 Yes 1413 0.688 Yes 1438 0.198 - 1476 0.661 Yes 1513 0.284 - 1578 0.674 Yes 1792 0.178 - 1813 0.622 Yes 1911 0.664 Yes

Explain the output -- Processing locus 133: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAACAAGTTAAAAATATATAATCATCTGTTAATATGGCATTAACCATTCCCGCCAAATTTCCAAATAAGTTGTATAAC AAAAAAAAAGAGCAGACTTGAGAAAAAGCCCATCAATATTCGAATGTGATAGTACACTTTAGGCATTTAGGCCTTTGGCC CAAACTAAGTATGTTGTCTCCTTCGCTGCCAAGGATGAACATTCTTGGTTTCACGGTGAACTTCTTGATCCGATGCAAAG TCTTACCCAGAAGAAGCAATACGAGTTCTCTCTCCCGAAACATCTCCGTACTCGCCAGTTATGATCAGGAGGAAGTATCA CCGGGACGATATAGCAATGAGTTTTCGAATCGTAACTTGGTGCACGAAATTTTACAGCTTTGTGCTAGAAACGGAGCTGT TATGGAAGCAAAAGCTTGCCATGGGAAGATTATTCGTATTGATTTGGAGGGAGATGTTACTCTGTTGAATGTTCTTATTA ATGCTTATTCCAAGTGTGGTTTTGTGGAGCTTGCCCGCCAAGTGTTTGATGGAATGCTTGAGAGAAGCTTGGTTTCTTGG AACACCATGATTGGTTTGTATACACGGAATAGAATGGAGTCAGAAGCATTGGATATTTTCTTGGAGATGCGAAACGAAGG GTTTAAGTTTAGTGAATTCACTATATCGAGTGTTCTTTCTGCTTGTGGGGTAAATTGTGATGCCTTGGAATGCAAAAAAT TGCATTGTTTGTCAGTGAAGACATGTATTGACTTGAACTTGTATGTTGGTACTGCTTTGCTTGATCTTTACGCAAAATGT GGGATGATCAAGGATGCAGTGCAGGTTTTCGAGTCTATGCAGGACAAGAGTTCAGTTACATGGAGTTCTATGGTGGCAGG TTATGTTCAGAACAAGAATTATGAAGAGGCACTCCTTCTTTACCGTCGAGCTCAGAGAATGAGTTTAGAGCAGAATCAGT TCACATTATCTTCAGTAATTTGCGCTTGTTCAAATCTGGCAGCTTTAATAGAAGGGAAACAGATGCATGCTGTTATATGT AAGTCCGGGTTTGGTTCTAATGTCTTTGTGGCATCCTCTGCAGTAGATATGTATGCTAAATGTGGAAGCTTGAGAGAGTC TTATATTATTTTCTCGGAAGTGCAAGAGAAGAATCTTGAGCTTTGGAACACGATCATCTCGGGGTTTGCAAAGCATGCTC GTCCTAAGGAAGTGATGATCTTGTTTGAGAAAATGCAACAAGATGGAATGCACCCAAATGAGGTTACTTTTAGTTCCTTG TTATCGGTTTGTGGTCACACGGGTTTGGTCGAGGAAGGAAGGAGATTTTTCAAACTCATGAGAACCACTTACGGTCTGTC GCCTAATGTGGTTCATTACTCATGTATGGTTGATATTCTTGGTCGTGCTGGTCTATTGTCTGAAGCGTACGAGTTGATAA AGTCTATTCCTTTCGATCCTACAGCTTCCATTTGGGGTTCTCTTCTAGCTTCTTGTCGAGTCTACAAAAATCTCGAGCTG GCAGAAGTTGCAGCCGAGAAACTATTCGAATTAGAACCAGAAAACGCCGGTAATCACGTCTTACTATCCAATATATACGC GGCAAACAAACAGTGGGAGGAAATCGCCAAGTCAAGGAAGCTTTTAAGGGACTGCGACGTGAAGAAAGTAAGAGGAAAAA GTTGGATTGACATTAAAGACAAGGTCCATACTTTCAGTGTTGGAGAGAGCGGTCATCCGAGAATCCGTGAGATCTGTTCG ACGCTAGACAATCTAGTAATCAAATTCAGAAAGTTTGGATATAAACCAAGCGTAGAACACGAGCTGCACGATGTGGAGAT AGGAAAGAAAGAGGAGCTTTTGATGCAGCATAGTGAGAAGCTGGCATTGGTTTTTGGGTTAATGTGTTTACCAGAGAGTT CTCCAGTGAGGATTATGAAGAATCTGAGGATTTGTGTGGATTGCCATGAGTTCATGAAGGCTGCATCAATGGCTACAAGA AGATTTATCATTGTTAGAGATGTTAATAGGTTTCACCATTTCAGTGATGGTCATTGTTCTTGTGGCGATTTTTGGTGACA AAGACAAACTCTGTTTTATTATTGATCTCACAGATAAATAAATTATTTTTTCCAACGAATGTTTTAAATTATGATTGCGT CAATTTGTTGGGCTTAT ...................................i............................................ ...........................................N.................................... ..........N.......................N.....................................N....... ............................................................N................... ................N............................................................... N...............................................N....i.......................... ......N..........................N................................N............. ...........................................................N.........N.......... ......................N...................N.................................i... ...N.........i......................N......................i.........N.......... ..N.................N.....................................N..................... ..............................................................i...N.........N... ...................N............................i...N......N.................... ..........................................................................N..... ..............N.................N.........N....i.........N...................... .........................................................N...................... .....N...............N...N...................................................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ......................................................................N......... ......................N......................................N.................. ..............N..............................N.......N..............i........... ...................N..........................N................................. ..........................................................N...........N......... ................. Pos Score Pred ------------------------ 36 0.651 Yes 124 0.124 - 171 0.145 - 195 0.427 - 233 0.241 - 301 0.258 - 337 0.222 - 402 0.417 - 421 0.248 - 454 0.085 - 469 0.131 - 481 0.099 - 529 0.185 - 534 0.522 Yes 567 0.326 - 594 0.425 - 627 0.266 - 700 0.151 - 710 0.376 - 743 0.497 - 763 0.189 - 797 0.662 Yes 804 0.384 - 814 0.549 Yes 837 0.345 - 860 0.706 Yes 870 0.346 - 883 0.114 - 901 0.307 - 939 0.491 - 1023 0.639 Yes 1027 0.143 - 1037 0.373 - 1060 0.300 - 1089 0.543 Yes 1093 0.111 - 1100 0.370 - 1195 0.173 - 1215 0.448 - 1233 0.243 - 1243 0.257 - 1248 0.658 Yes 1258 0.316 - 1338 0.423 - 1366 0.115 - 1382 0.456 - 1386 0.444 - 1831 0.284 - 1863 0.120 - 1902 0.353 - 1935 0.244 - 1966 0.237 - 1974 0.237 - 1989 0.650 Yes 2020 0.372 - 2047 0.228 - 2139 0.075 - 2151 0.078 -

Explain the output -- Processing locus 134: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATAGGTGAGTTCCGATCTCCTTTTCCCGTTTCGAGAACCCTAATCTCATCATTTCTCAAACCACTTATCTGTCTCCTT CCTCGAATTTTCTAGATCTGAGCTTCTTCTTTGACTATTCACCGATCCGGATCTAAAAAGGTGTTTCCTCAATTTGTTAA ACCAGATATGAGGTCAATGGATCGATTTGGGATCTGGGTTTTGGCGATTTTGTTAGTGATTCAATCAAGTTTTGGGTTTT ACCTTCCTGGAAGCTATCCACATAAGTATGAAGTTGGTGATTACTTGAACGTGAAGGATAGTGCTGAGAATTTAGGTGAA CTTCTTATGGGAGATCGGATTGAGAATTCGCCTTATAGGTTTAGAATGTTTAAGAATGAGAGTGAGATCTTTCTTTGTCA GACTGATAAATTGTCTGCTGATAGTTTGAAGCTTTTGAAGAAGAGGATTGATGAGATGTATCAAGTTAATCCTATGCTTG ACAATTTACCGGCGATTCGGTATACTAAGAGAGATGGGTATGTTTTGAGGTGGACTGGTTATCCTGTTGGGATTAAGGTT CAGGATGTTTATTATGTTTTTAATCATTTGAAGTTTAAGGTTCTTGTTCATAAGTATGAAGAAGCTGCTAATGTGGCTCG TGTGATGGGTACTGGTGATGCCGCTGAGGTCATTCCGACTATTGGGAAGAAAGATTCTGATGTCCCTGGTTATATGGTTG TTGGGTTTGAGGTTGTTCCTTGTAGCTTTGCTCATAATGGTGAATCTACGAAGAAGTTGAAAATGTATGAGAGATATACT ACTCCGATTAAATGTGATTCCACTAGGGTGTCTATGTCTGTTAAGGAAGGTCAGTCTATTGTCTTCTCTTATGAAGTCAG TTTTGAGGAGAGTGACATCAAATGGCCATCTAGATGGGATGCGTACTTGAAGATGGAGGGTTCGAAGGTTCATTGGTTCT CAATCTTGAACTCTCTTATGGTAATCACTTTCTTGGCTGGTATTGTTCTGGTGATATTCTTGAGAACTGTTAGGCGGGAT TTGACTCGTTATGAGGAGCTTGACAAGGAGGCTCAAGCTCAGATGAACGAAGAGTTGTCTGGATGGAAGCTTGTTGTGGG TGATGTTTTCCGTGCTCCATCAAATGCCTCGCTATTGTGTGTCATGGTTGGTGACGGAGTTCAGATTCTTGGCATGGCTG TTGTTACCATTTTGTTTGCTGCTCTAGGGTTCATGTCACCGGCTTCACGTGGAACACTTATTACTGGTATGCTCTTCTTC TACATGATTCTTGGCATTGCAGCTGGTTATGTTTCTGTTCGTCTTTGGAGGACAATTGGTTGTGGCGAACACCGTGGATG GATGTCTGTCGCATGGAAAGCTGCTTGCTTTTTCCCGGGTATTGCGTTTCTGATCCTTACAACGCTGAACTTCCTTCTAT GGGGTAGCCATAGCACTGGAGCCATTCCTTTCTCTCTATTTGTCATCCTCCTCCTCCTGTGGTTTTGCATCTCGGTTCCT CTAACTCTTATCGGTGGTTACTTTGGAGCCAAGGCTCCTCACATTGAATTCCCAGTTCGAACAAACCAAATTCCCCGGGA AATCCCCGCTCAGAAATACCCATCCTGGCTTCTGGTTCTTGGCGCTGGAACACTTCCATTCGGTACCCTCTTCATCGAGC TTTTCTTTATCATGTCCAGCATCTGGATGGGCCGTGTCTACTACGTCTTTGGATTCCTTTTTGTAGTCTTGATCCTTCTT GTCGTAGTGTGTGCCGAGGTTTCCCTAGTGCTAACATACATGCACCTGTGCGTTGAAGACTATAAGTGGTGGTGGAAATC CTTCTTTGCATCTGGATCCGTTGCAATCTACATCTTCATATACTCCATAAACTATCTCGTCTTCGACCTCAAAAGCTTGA GTGGACCCGTTTCAGCGACTCTTTATCTCGGCTACTCTCTCTTTATGGTCCTGGCTATCATGCTCGCAACAGGTACCGTT GGTTTCCTCTCTTCCTTCTGGTTCGTACACTACTTGTTTTCTTCGGTGAAACTTGACTAAGGAATCAGACCAGAGGCTTA TTGGCTTAAAGAGATTGTATAACTTTTTGGAGAGAACTGAGATATCGTTTAAGCAAAGGCATCAATCAAATCATTCAAT ................................................................................ ................................................................................ .......N........N............................................................... ...........................N.................................................... ......i......................................N.........N........................ ..................................................N....i.................N...... .................................N.....N........................................ ....N........N.........................................N..............N......... ....i............N.........................................N.............N...... ....................................N.........................N...N............. ...........N.....................N....................................N......... .....................i...........N....N.............i........................... .................N.............................................................. ..........N...............................i...................N................. ..N....................N...................i.............................i...... ................................N...................................N........... ...i........................N................................................i.. ................................................................................ ................................................................................ ................................................................................ ...........N..............i..................................................... .......................................N........................................ ................................................................................ ............................................N..............N.................... ................................................................................ ............................................................................... Pos Score Pred ------------------------ 168 0.227 - 177 0.394 - 268 0.185 - 327 0.539 Yes 366 0.243 - 376 0.150 - 451 0.203 - 456 0.610 Yes 474 0.238 - 514 0.176 - 520 0.074 - 565 0.173 - 574 0.108 - 616 0.110 - 631 0.171 - 645 0.751 Yes 658 0.053 - 700 0.095 - 714 0.413 - 757 0.234 - 783 0.475 - 787 0.170 - 812 0.403 - 834 0.383 - 871 0.147 - 902 0.680 Yes 914 0.414 - 919 0.082 - 933 0.530 Yes 978 0.380 - 1051 0.217 - 1083 0.622 Yes 1103 0.197 - 1123 0.052 - 1144 0.080 - 1164 0.593 Yes 1194 0.717 Yes 1233 0.447 - 1269 0.330 - 1284 0.510 Yes 1309 0.272 - 1358 0.561 Yes 1362 0.648 Yes 1373 0.344 - 1439 0.547 Yes 1692 0.475 - 1707 0.783 Yes 1800 0.317 - 1965 0.271 - 1980 0.406 -

Explain the output -- Processing locus 135: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATTCAATAGAAGAATTTTGGTATAGTTCGTACAGATGGAAGTTGAAATTAGTTGAATTGCCGTTTTCTATACGTACGA ATTTTGGTATTTATTGATTAGATAAACCGGAACAAAACCGGTTTAGTAAACCGACGCATGGTGTTGGTAAAGTGTGATTG GGTAAGAAGCCTAACTAGTTGTCTGTTGATAATTGTCACTAGGTGGGCACTTAAAGAAAAAAACACTTGTTTCCTTATAT ATTCTTCTCCCTTTAAACATTTAATCTTTTCCTCTTCTACCATCTCCACAAATTCCAAACATCTCTCTCTCTTTCTCTCT CACACACAAAATTGCAGAAGAAGAAGAGTCATGAATGGTGAAGAAAGCTTTGTAGAAGATTGCTCTGTTTTTGTTGAGAT TGATCCTTCTGGAAGATATGGAAGATACGATGAAATACTTGGCAAAGGAGCTTCAAAGACAGTATACAGAGCATTTGATG AGTATGAAGGTATAGAAGTAGCATGGAACCAAGTAAAGCTTCGAAATTTCACAAGGAATCCTGAGGAATTAGAGAAGTTT TTCAGAGAGATTCATCTTCTCAAGACTTTGAATCATCAAAACATTATGAAATTCTACACTTCTTGGGTTGATACCAACAA TTTATCAATCAATTTTGTCACTGAACTCTTCACCTCTGGTACTCTCAGACAGTATAGGTTGAGACATAGAAGAGTGAATA TTAGAGCAGTGAAGCAATGGTGCAAGCAGATTTTAAAAGGGCTTCTTTATTTACATAGTCGTTCTCCACCAATTATACAT AGAGATCTCAAATGTGATAACATTTTCATCAATGGAAACCAAGGTGAAGTCAAGATCGGTGACCTTGGACTCGCTGCGAT TCTTCGTAAATCACATGCCGTTCGTTGCGTTGGTACTTCAAAACCTTCACATCATTGGAACTTTATTGCTTTAATTATGT TTTTTACAACTTTGGACCTACCTTTACTATGTTTATGTGTTGTTAAAGGAACCCCTGAGTTTATGGCTCCAGAAGTGTAT GATGAGGAATATAATGAGTTGGTTGATGTATATGCTTTTGGCATGTGTGTGTTGGAGATGGTTACTTTTGATTATCCTTA CAGTGAATGTACTCACCCGGCACAAATCTACAAGAAAGTTACCTCGGGGAAAAAGCCTGAAGCTTTTTACTTAGTGAAGG ATCCTGAGGTTCGTGAGTTTGTTGAGAAGTGTTTAGCTAACGTGACGTGTAGGCTAACGGCATTGGAGCTTTTACAAGAC CCTTTTCTACAAGATGATAATATGGATGGATTTGTTATGAGACCTATTGATTACTACAATGGTTATGATGAAACTGGTGT GTTCCTTAGACATCCTTTGATTGATGATCCTCTTTACCATGATCAGTTTGAGTCGTCACAGATATGTGAGATCGATCTTT TCGCTAACGATGATGAAGATCATGTCGACATTTCGATTAAAGGGAAGAGAAACGGTGATGATGGGATATTCTTGAGACTT AGAATATCTGATGCTGAAGGTATAGTTTCAATCTTTTTCGATAGTTTTAAAATCATCGGTCTTAAAAACGTAACACGCTT TCGAGTTTTGGGTAATGTAGGACGGATAAGGAACATTTACTTCCCGTTTGAGACGGCTATTGATACTGCATGGAGTGTAG CGGTTGAGATGGTGTCAGAGCTCGACATAACGAATCAAGATGTTGCGAAAATCGCGGAGATGATCGATGCAGAGATTGCT GCATTGGTGCCTGATTGGAAAAATGATACAGAAAGTTCCCAAAATGTAAACAACAACAAGAACAACAACACTGCAGGATT CTGTGGAGAGTGTGCTTCAAACGGGTATATACAAGAGACTGTATCATCAGGAGAAAAATCTCATCATAATCATCATGAGT TCGATAGTTCTGAAGACAAGAGCTGTTCTTCGGTTCACGGTAGGTTTGCGGATATGTGGGGTTTGCGAGAATCATATTCT GATGATGGAGAAAAACAGAGCTCAAGGAAGGTTAGAAGTGGACGGTGGTCGGAGAATGAGATGAGACGAGAACTGAGATG GCTTAAGGCAAGGCACAAGATTCAACTTATGAAAATGAGAGGTCAAACGATCTGCGAGACACCGATAGAGATCTCTCTTA CACCGGGAACTTCAGTTTCGTTACCTCTTCTTTACAGGGCTATATCACTTCCTGTGGATGCCGTGGATATGTGACATTAT TGTAAAGTCTGATGATAGTTATCACTTATATTTGTATGTTTCGACATTTTTAACTTTTGTTAAGTTAAAAGTTAATGACA TAGTGTACTTTAT ....................................i........................................... .........................................................N...................... ................................................................................ ................................................................................ ..............................i...N............................................. .................N...........N...............................................N.. ...N..................N......................................................... .............................................N.................................. ................................................................................ ................N............................................................... ...........N...................N................................................ ..............N.............................................................N... ............................N.....N...........................N...............N. ......N......................................................................... ................................................................................ .............N.......i...N..........i.....................N.....N..N............ .......................N..............N........................N................ .........N..N........N...................................N..N................... ..........N..................................................................... ..............N......................................................i.......... ........i..............................N...................i......N............. ......................N....................N.................................... ..........................................................................N..... .....................................................i.......................... ............................N.....N............................................. .........................................................N..........N........... ...........N.......................N......................................N..... ............. Pos Score Pred ------------------------ 37 0.708 Yes 138 0.238 - 351 0.700 Yes 355 0.497 - 418 0.241 - 430 0.144 - 478 0.109 - 484 0.170 - 503 0.400 - 606 0.328 - 737 0.385 - 812 0.312 - 832 0.319 - 895 0.097 - 957 0.340 - 989 0.149 - 995 0.159 - 1023 0.493 - 1039 0.148 - 1042 0.210 - 1054 0.310 - 1066 0.193 - 1072 0.153 - 1083 0.439 - 1098 0.656 Yes 1127 0.293 - 1294 0.073 - 1302 0.733 Yes 1306 0.251 - 1317 0.508 Yes 1339 0.325 - 1345 0.180 - 1348 0.129 - 1384 0.081 - 1399 0.104 - 1424 0.218 - 1450 0.250 - 1453 0.081 - 1462 0.150 - 1498 0.162 - 1501 0.379 - 1531 0.212 - 1615 0.288 - 1670 0.610 Yes 1689 0.819 Yes 1720 0.222 - 1740 0.597 Yes 1747 0.321 - 1783 0.364 - 1804 0.337 - 1915 0.460 - 1974 0.597 Yes 2002 0.248 - 2005 0.244 - 2056 0.383 - 2061 0.210 - 2078 0.528 Yes 2109 0.188 - 2115 0.274 - 2218 0.283 - 2229 0.157 - 2252 0.062 - 2276 0.029 - 2315 0.051 -

Explain the output -- Processing locus 136: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAATAAAAATCAATAATATTATTCCAAGAATGGCACGAGTGATTATTTCTTCTCAAATAGTGGCATAAGAGGTGAGCCT TCCAAATTCTTCAACCCTTAAACCCATTCATTTTCTCCAGAAGATTCTGAGACGAAGAGGCAAAATTTTCTCGGGAAAAT GGCAATGGAAGAATCCGATTCCGGTGAAACAGCAACGGCAAAAATTTCGTCTCTTACGTTGAAATCTAGGGCTATACTTG CGACTCCAAGCAACGAAGAAATAGCGATTATCGTCGAACAAGTCTATACACCTCAAGAATCCGACGAACACAAATCAGCG CGAGCTCTGTTCGATTTCTGCGTCTCTACTTTCCCAAATTGCTTAACCCTAAAGCTTCTCACGGTGTATCGATTTTCTTC CGACGACGATTTCCGATTACGATCGATCTGTCTTCTAACTGAAACTCTAAAAGCTCAGCGATTCGAACTCTCTCTTGTCA CTCTCCATGAAATCAAACCACTTCTGATTTCTTGCTTGACAATGCAAAACCCTAAATATTGTGACTCAAAGATTGTGAGA ATCATTGTTTCTTTAGTAGCTGATAAAGTTGGTGTCTGGGAAGAGATGAGTGATTGTATACTCTCACTTGTTGTCAATGA TCCGATTAGGGCTTTTGAGGTCTTTATCCAATTGCCATCATCACTCTACGGAGAATTCATTTACCGGTTTCTGCAGAATT TTCTTGATGAAGTTTACAAGATTTTGCTTCGTCCTGTGGAATATGAAGTTGAAGTTTGGATCTTGGCTTTGAAATCTGCT GTTAAAATGGGGATTTTGCTTATGGATTCAGAAATGAGATTTGATTTGACAAGAGACATTCTTCACATTGTTTGGAAATG TTCTCTTGATGTTGTGTGGAATGATATGGAAGAAGAGTTTCTGCTAAATGGACTTGATTCTCTTGAGATTTTCTTGGTGG AAGACGCGAACAAGTTTAAGTGGAATAGTGATCAATGCAGGTTTGTGGCAGAGTTTGCGTTTAAGATTTCAGATATCGGA AAAGAGGCCAAGGAAGTTGCTTGGAAGATCTATCGAATGGTTACAAAGCTGGATAAGTATGTGCACAATCCAGCTTTCGA ATTTAGCCCTTTTCGCAATGAGAATAAATATCTTGGAGTAGATGATGTTTGTGACTTAGAAGACATTTTGGATGCTTTAT CTCCAGTGGAAATCATGAGAAAGGTTTCAGTAGGTCAAGTTCCTAATAGGTTGAGAGAGATAGCTATCACGAGACTTTAT GAGTTATTTTGTGATCACACTGCTGGGAAAGGGGATATAAGTATTTCAGAGATTAGAGAAATCCAGCCGTTGCTCATCTC TTGCCTTGCAGAGATAGGAATTCCTGAGAGCACGTTTAAGATTCTCTGTCAGGTGGTGTTCCATGTTTTACATGAGTTGT CTAGCTACCAAGAGGACAACTGGTTTGGTCTATGGGACTACATTGCAACAGAAAGCAAAACAGAGTTCATGAAGACAGTT TATATCTTCCAGTGCTTAACAATGCGGCTCGATGATAAAGAGTTTGTGATTCATGCGATTAATAATCTTCTTCCAGAGAT TCACAGACACCTAAACCCACCAAGAGACTTGTTGGTAGATGAAAATTGCTGGGTCTTGGCGTTTACGGGTGCGTTCTGTG CAGCAATTCACTTGATAGAGATCTCAAGTCATGCTCAATACTTGAAGGAGATTGCATATAAGATGATAGATTCAGTGAGG GAGCTTGTGGGAAGAGGAATGGAAGTTGAGCTTGTGAGGAGAGCTTTCATAAATATGGAAAGCATTGTGGAGAAACAATA TGACTGTTACACTACTAGTGACTACAGATTCGTTAAGGGTTTGGTTTGGAAGCTCTATGCAATCAAAGACATCAGCGTGG AAACTCAGTGTGTGTTGTGGAGAATCAACGTGATTCTGGAAAAAGTGCAGGAGGTAAAAGAGCTCCCAAAGAGTGACCTT GATTGGCTGAACCAACCCGAGACTTTAGGCAATTGAGTTTTTTTTTTTTTTTCTTCATAGTTTGGTTTGTGTAGGAGAAG AAAAGAAACCTATGTTCCTAACTCTCATATCTACTGCTTTCTTGTCTACTGTTTT ..............................i................................................. ..............................................................................i. ....i........................................................................... ................................................................................ ................................................................................ ................................................................................ ......N..................................i...................................... .............................................N..............................N... ................................................................................ ......N...................................N..................................... ......i..............i...........N...........................................N.. ........N...........N....N.....................N................................ ..................................N............................................. ....................................i.....................N..................... .................N.......................N..N..........................N........ ..............N...............................................................N. ................................................................................ ..............................................................N........N........ ...............................N....................................i........... .....................i.........N....................N........................... ......................................N......................................... ..............................N...............................N................. ..................i...................................N........................N ........................................................N....................... ................................................................................ ................................................................................ ...........N........................................... Pos Score Pred ------------------------ 31 0.629 Yes 159 0.829 Yes 165 0.789 Yes 487 0.162 - 522 0.636 Yes 606 0.421 - 637 0.124 - 727 0.082 - 763 0.165 - 807 0.604 Yes 822 0.510 Yes 834 0.329 - 878 0.388 - 889 0.071 - 901 0.261 - 906 0.487 - 928 0.121 - 995 0.305 - 1077 0.504 Yes 1099 0.378 - 1138 0.114 - 1162 0.132 - 1165 0.267 - 1192 0.069 - 1215 0.410 - 1279 0.112 - 1423 0.179 - 1432 0.201 - 1472 0.418 - 1509 0.548 Yes 1542 0.737 Yes 1552 0.344 - 1573 0.138 - 1639 0.194 - 1711 0.186 - 1743 0.355 - 1779 0.659 Yes 1815 0.168 - 1840 0.486 - 1897 0.129 - 2092 0.067 -

Explain the output -- Processing locus 137: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAAACTTGTAGGTTAGAAAGGAGATGAAATGAGAAAGTTAGGGGCTTTTCTACAATTGTCTTCGTTTATGATCACGAA TTGAATAGTTGACAAGTCTAAAATTTGTCTCTTTCCTTGTCACTTGTCAGTGAGTATACAAAACAATACACAGAGCCTCG CATGTCATATTCACACAGGGACCTTCCGTTAAACTCCTTTTTTATAATCTCGTTCTCTCTTCTCTCTTTCTCTCTCTGGT TGCTCTTGGCCATGGTTGTGAATAGTCAAGCTTTCTTGTTGGCATTAATTGCATTACTTGCTACCCAATTGCCTTCACTA ATGGCTGCAGATTGTCCATTAGATTTCAGTGGATCAAATTTCACTCTAGTAGCTACTGTGTGCTCCAACATAACCAACAG AGGCAAATGTTGCCGCTACATGAACGCTTTTGTTGCGGTGTCCGTGGCTCGTTACGCGAACTTGTCAACTAATCTTGGAG TCACATCAGATTTATCTGAAACCTGCATTGCTTCCATCTCTAGAGCCATGGAGGGCTACGGAGTCTCGAGAAACGCCACT AGTTTCTGCGGTTTGGGAACCAAGATCCTTGTTAAGTATGACTGTGACGGTCGAACCACTGTTACACAAATGCATCAGTC TCCGGGATTTGGACATGTTTCAAGAAACTGCAGACTCCCATTTTCGCCGGGACACCAATGCAGAAAGTGTTTGAACTCTG GCATCACTTACCTTCGTAATCTCATAGGTGCAGAGACTAATAACATTACTCTGTGTACTTGTCGTGATGCGACTTATGCT ACATTAGCAAGCCGAATAGACGATACATCAGCTCTTGAACTCCTTAGTTGTTTCTTTCAAGTGACAGAGCTTAACATTCC TTCAGAGTCATTTTCACCAGTGGCAAGTCCAGAACCTTCTCCAAGCACGGTTGGTGGTATTAGCCCAAGCAACAGTGATT CTCAAATGACTACAAGTAGAAGCACCAATCCATATCACTTAACAATGGTTCCAACCATTGGGATTGTTGTTACAGCTGTT GCTCTTACGATGCTTGTAGTCTTGGTAATTCTTATCCGTAGAAAGAACCGGGAACTGGATGAATCTGAGAGCTTGGATAG AAAGTCAACAAAATCAGTTCCTTCTTCCCTTCCTGTATTCAAGATTCATGAAGATGATTCTTCCTCAGCTTTCAGAAAGT TCAGCTACAAGGAAATGACAAATGCAACAAATGATTTCAACACGGTTATCGGTCAGGGAGGTTTTGGTACTGTTTACAAA GCCGAGTTCAACGATGGTCTGATTGCAGCGGTAAAAAAGATGAACAAAGTCTCTGAACAAGCTGAACAAGATTTTTGTAG AGAGATAGGGCTTTTAGCTAAGCTGCATCACCGTAACCTCGTTGCTTTGAAAGGCTTTTGCATCAACAAGAAAGAAAGGT TCTTGGTCTACGACTATATGAAAAATGGCAGCTTAAAAGATCACTTACATGCCATTGGAAAGCCTCCACCTAGTTGGGGA ACAAGAATGAAAATTGCAATTGATGTGGCAAATGCTTTGGAATATCTTCATTTCTACTGTGATCCTCCTCTCTGTCATAG AGATATTAAATCAAGCAATATATTACTCGATGAGAACTTCGTCGCTAAGCTTTCAGATTTTGGCCTTGCACATTCGTCTA GAGATGGCTCTGTTTGCTTTGAGCCTGTGAATACCGATATCCGTGGAACTCCAGGTTATGTAGATCCGGAATATGTAGTA ACGCAAGAGTTGACAGAGAAGAGTGATGTGTACAGCTACGGAGTTGTGTTGCTGGAGCTCATAACCGGGAGAAGAGCAGT TGATGAAGTTGTGACGGTTGTGAGATTGTGTACCGAGAAAGAAGGCCGGTCTAGGCCATCAATTAAGCAAGTCTTGAGGC TGTTGTGTGAAAGCTGTGATCCGGTGCATAGCGCATTTGCTAAAGCGGTTGAAGAAGAGATAGGCTGGGATAGTAGGAAG AGAAGCAATTTGAGAATTCAAAGAGGTGATTCCCGGATCTTTGGTCCATCGTCTAGTACTACTTCCAGGTCACATTACAG CCGGAGTTTACCTCACTCTCCAATTAACGGATTTTCTTTCTGAGTTAGTCACATCTTCTCTATAGCTAAATCTGAAAGTT TTACATATGTTTATGAAAATTATGAATTCATATTGAAAGCTCTCATACTCTACACAGACTGC .........................N....N......................................N.......... ................................................................................ ...........i.................................................................... i............................................................................... ......i............i............................................................ ...............................................i................................ .....................................N...............................N.......... ..............i..........................................N...................... ..................................................................N........N.... ................................................................................ ................................................................................ .....i......................................i................................... .........N................................................N..................... ...............................................N.....N.......................... ..............i......N........N................................................. .............N.........................N........................................ ................................................................................ .................N......N.......................N............................... ......i...............N........N................................................ .............................N.................................................. ...N.....................................................N..............N....... .........................N...................................................... ..N............................................................................. ................................................................................ ................................................................................ ................................................................................ ......N.....N........N........................................ Pos Score Pred ------------------------ 26 0.116 - 31 0.442 - 70 0.179 - 162 0.217 - 252 0.707 Yes 321 0.644 Yes 407 0.510 Yes 420 0.533 Yes 528 0.831 Yes 598 0.488 - 630 0.249 - 655 0.663 Yes 698 0.128 - 787 0.074 - 796 0.115 - 966 0.787 Yes 1005 0.863 Yes 1050 0.308 - 1099 0.141 - 1168 0.309 - 1174 0.376 - 1215 0.889 Yes 1222 0.263 - 1231 0.357 - 1294 0.249 - 1320 0.409 - 1458 0.370 - 1465 0.473 - 1489 0.185 - 1527 0.582 Yes 1543 0.215 - 1552 0.306 - 1630 0.354 - 1684 0.402 - 1738 0.252 - 1753 0.131 - 1786 0.258 - 1843 0.342 - 2167 0.142 - 2173 0.053 - 2182 0.051 -

Explain the output -- Processing locus 138: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAGAGGTGTGTAAGCTCCTTCTTGTTCTCTTTATGAGATGTTTGTCAGCATATGCTTCCCATTGTGCTTACACTTTTAA TTTCATCTTTGTTGCACCTGAAGGCAAAAACAATATTTTGAGCAGAGGAGACAGCAACAGCATCAGTTCACAGTTGGGTC TGAGAGCTGTAGTAATGATATAAACAATAGTAATCAACACTTAAGAGAACACCAATCTCTGGATATTCTCAATTTGCTGA ATTTGTCAACCGCTACTCCTGAATGCAAACCTTCTGGTCCAGAAAATGGTAAGTATATGCTTGTTATGACTTCCAGCAGT CATAATTGGAGTCAGTTTATGAACCATTTATTTGCTTTATCGCTCAGAACCATGACATGTAATTTCACTTTGCATTTTTC TTGTTTGTGTCACTTGTTAACGGAAAAAGGAATGCAAGATCTGGATGCTGATTTTTACAGCCTTAAGGACAACATGTCCG GAGTAGGAAGTTCATTTAACCATATAGCAGAACCTACTTCCTCTAAGAGGACTTTGTTTAGTATCCCTGACAACCAGACA AATGATTTCAAGAAAGCAAACACTGACAACCAGACAAATGATTTCAAGAAAACAAACACCACCGCAGATCTGATGGATGG CACTGAGCGGAAGTTATCTGTCTTTGATTTGGTTGGTGATGATCATACAACAACCAACCTGGAAGAATGTTCTCCGTCTG AGGCTCACATGGCATTTTCAGTCGAAGGATTAGGTAAAATAAACACGGAAACTCCAGTTAATTCCCCACAACCATCAGAC AGAACTTTTGTGTACCGGTGCTCCTCTCCATGGAAGGATACTGGTCAACCAGATACATCACATGTCAGGGGAAGGCTCAA CGACTTTGAGAACGAAGTGGACACAATGATTCAAAGTAGCAAAATGTTCCAAGATGACAGCCTTTATAGGTCTCCAATTG GCATACATGCTAAAGATGGGGGCAGAAAGCAAAAACTGCAAACTTTCAGTGATCACTTGCACAAACAATATAGCGACTCT AGAAATTACTTCTGTGACGTTGCAGATTTCAACAACAGCAGGTTCTCTGACGATGAATGGAATGCTAAGCCCGCCTTTCT TGATGACGGGGAAGATAGTTTTTACTGGAAGGCCGAACAGCCATGCCAAAAGGAGAGTTTGAACCCTGATTTCCTGAAAT ATTGCAATGATTGCACTGAGAGTCGCTCTTCTACAGAACACCATAGGAAAAAGAAGAGGGATTACCTGGAAACGACCTGG AGGTCCAACATTAGAGATTCACCAACTCGAAGATCACATCTTTTAAAAAGGAATATCGATTATCCAAGTTTCGCAAAGGC AGCAACCTCCGACTTTGATTTTGACAATGTATTTGACCGACCGGTTTGGTCATCCATTGTACTTGAGGAAGATAAAGACA GCCATAGTTTGCGTAGCGAAGAATCTTGCTCGTCTAGTGCAGTTTGGACCAATGAAACCCACAATTCACAGTTTGAGACC AACACAAGACAAAGGAAAAGGGAAACTAATAAGTTTAGCAACCTTGGAGACAAAAAGTATATCAATACTGACCTTTTCCA AGAATCGTGGGAAGACTGGGAAGTGGATGATCAACATATGAAGAGACAAGTTAGGTCTGGAAAGCAGGGCAGACTATCAA ACTCAGGAAAGTTAAAATCTACCAGTCAGAGAAAGGGTGGTCTAGACGCGTCTTACGATTGGTTTGGAGAAGGATTTACA TCAGCAGGAATAAACTCAGAGATTACAAGTGAGAGGAATAAGCCATATCCTTTCCTAAACCCTGAGCGTGGTTCCTCTCA CTGGCGTTCATCTCGTGCCCCTGATTCTATTCCGGAAACATGGATTCCCAAGTTTTCAGTAGGAGGCACCGGAGATGATG ATGATGGTGACCACGATGAAGAGGACTATGTAAACTGTTTATCAGCAAACCATAAATCTAAACTAGCAGGAGACACATGC GGTTTTGAGAATGACACGTTATCAGAGAACGATAATGAGCAAAGCAGAGAAGTAAATCACCCAAAAAATCAGGGAGATGA GACCTCATCATCCATTGCAAAGAGCCTCTCAGACGAAAATGATGTTGTTAGATGTAACCCCAACAAGGAGGTCATGGAAG CTCGTCACCAAAGAAACAGAGAATCAGGAGAAAAGACAAGTAGAGACCCGTTCCAACAAATGATAATGCTGGAAAGACGC ACACTTCAGCTCGTTTGTTTTAACAAGGCATTGCTTCTTGACTCTTTAAAAACTTAGGTAAGAAATTCATATCCATATCT TGGGTCTTTAGTTCAAGTTATCACCAAATCTGATATGTTTCTTAAGCATAAGGAAAAAAGTGTTATTTTTGGTTAT .................................N....N.............N........................... ................................................................................ ..............N................................................................. ......................N......................N..........N........N.............. ..................N................................i....N....................... ...............................i............N............................i...... ................................................................................ ......................................N...........................i............. ........i....................................................................... .............................N...............................N.................. .........................N.................i.........N.......................... ......N........i................................................................ ....................................................N...N....N.................. ..N.......................................N..................................... ......i......................................................................... ................................................................................ ..........................N..................................................... ...................................................N............................ ................................................................................ ..........................N..........i.......................................... ................................................................................ ................................................................................ .......................................N..................................N..i.. N..N...........N...........N................................................N... ..........N.......................N.........................................N... ......................................N..N.........i.....................N...... ...........................................................N.....N.............. ................................................................................ ..................................N......................................... Pos Score Pred ------------------------ 34 0.255 - 39 0.342 - 53 0.314 - 175 0.140 - 263 0.115 - 286 0.355 - 297 0.338 - 306 0.285 - 339 0.134 - 372 0.629 Yes 377 0.376 - 432 0.760 Yes 445 0.289 - 474 0.778 Yes 562 0.451 - 598 0.384 - 633 0.769 Yes 637 0.299 - 679 0.137 - 707 0.506 Yes 729 0.604 Yes 830 0.247 - 862 0.419 - 906 0.471 - 924 0.704 Yes 934 0.376 - 967 0.131 - 976 0.701 Yes 1093 0.469 - 1097 0.354 - 1102 0.353 - 1123 0.430 - 1163 0.370 - 1207 0.503 Yes 1387 0.302 - 1492 0.364 - 1627 0.205 - 1638 0.707 Yes 1880 0.488 - 1915 0.456 - 1918 0.634 Yes 1921 0.464 - 1924 0.435 - 1936 0.267 - 1948 0.236 - 1997 0.308 - 2011 0.456 - 2035 0.323 - 2077 0.281 - 2119 0.350 - 2122 0.413 - 2132 0.644 Yes 2154 0.282 - 2220 0.188 - 2226 0.336 - 2355 0.042 -

Explain the output -- Processing locus 139: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTCTTCTTTTAGCTTAAATGTCTCTGGTTAGTTATGTCTGAAGTATACAACACCGGTTCCAAAGTTCGTTGACCATTG TTTGAGTTCTTCTTTTATTAGGATTGTGAGAATAAGAATCGGATTATGCTAACTCTAATTCGTAATCTGCGTTTTACTTT AGGTTACTCAATGAAGCAATTCATGTCAGTGGTGAGATTCAAATTTGGTTTCACTTCAGTCAGAATGAGGGATTGGTCGG TGGGAGTCTCCATTATGGTTCTTACATTGATCTTCATCATCCGTTATGAACAATCTGATCACACTCACACTGTGGATGAT TCTAGTATAGAAGGAGAGAGTGTTCATGAACCCGCAAAGAAGCCACATTTTATGACTTTGGAAGATCTTGATTATCTATT TTCAAACAAGAGCTTTTTTGGAGAAGAAGAAGTGTCCAATGGAATGCTTGTATGGTCTCGAATGCGTCCATTTCTTGAAA GGCCAGATGCTTTGCCAGAAACTGCTCAAGGGATAGAAGAAGCTACATTGGCAATGAAAGGTTTGGTTTTAGAAATCAAT AGAGAGAAGAGAGCTTATTCTTCTGGTATGGTCTCTAAGGAAATTAGAAGAATCTGTCCGGATTTTGTCACTGCATTTGA TAAGGATTTGTCTGGTTTAAGTCATGTACTTCTTGAGCTTCCTTGTGGTTTAATTGAAGATTCTTCAATAACTTTGGTTG GTATTCCTGATGAACATTCTAGTAGCTTCCAGATTCAGCTCGTTGGCTCGGGATTATCAGGAGAAACTCGTCGGCCAATA ATCTTGCGTTACAATGTGAACTTTTCTAAACCATCGATAGTGCAAAATACATGGACAGAGAAGCTAGGTTGGGGAAACGA AGAGCGATGCCAATATCATGGATCATTGAAAAATCATTTAGTTGATGAACTTCCTCTCTGCAACAAACAGACCGGTAGAA TCATTTCGGAAAAGAGTTCCAACGATGATGCAACTATGGAACTTTCTCTTTCAAATGCTAATTTTCCATTTCTCAAAGGG AGTCCCTTCACTGCCGCATTGTGGTTTGGCTTAGAAGGTTTTCATATGACGATAAATGGGCGGCACGAGACTTCATTTGC TTACAGGGAGAAGCTCGAGCCATGGTTAGTCAGTGCAGTCAAAGTCTCAGGTGGTTTGAAAATTTTATCTGTCTTAGCCA CAAGACTGCCCATTCCCGATGACCATGCATCTTTAATCATAGAAGAGAAACTTAAAGCTCCATCTCTTTCCGGGACAAGA ATAGAACTATTGGTGGGTGTTTTCTCCACTGGAAATAATTTTAAGCGGCGTATGGCATTGAGAAGATCTTGGATGCAATA CGAGGCAGTAAGATCTGGCAAAGTAGCTGTTCGATTTCTCATTGGCCTTCACACAAATGAAAAAGTCAATTTAGAGATGT GGAGAGAATCTAAGGCATATGGAGACATTCAGTTTATGCCATTTGTTGACTACTATGGTTTACTTAGCTTGAAAACAGTT GCGCTTTGCATTCTCGGGACCAAAGTCATCCCAGCAAAATACATAATGAAGACGGATGATGATGCGTTTGTACGGATTGA TGAGCTCCTATCAAGTCTAGAAGAAAGACCGTCTAGTGCCCTTCTGTACGGTTTGATCTCATTTGATTCATCACCGGACC GTGAACAAGGCAGCAAATGGTTTATCCCTAAAGAGGAATGGCCTTTAGATTCATACCCTCCATGGGCACATGGCCCTGGC TACATCATCTCTCATGATATAGCGAAATTTGTGGTGAAGGGTCACCGTCAAAGAGATCTTGGACTTTTCAAGCTGGAAGA TGTGGCGATGGGGATATGGATTCAACAATTCAACCAGACGATAAAAAGAGTGAAGTACATCAATGACAAAAGATTTCATA ACAGTGATTGTAAATCAAATTACATTCTTGTTCATTACCAAACTCCTAGACTAATTTTGTGTCTTTGGGAGAAGCTGCAA AAAGAGAACCAATCTATTTGCTGCGAATAAATCTCTCGAGCATGTACGTATGTAACTTTTCTGGTTCTGTTTCACACTTC AGGAAGAAGTTCTCCCAGTTAAAGACACACTGGCTCGAGTATGTCAGAG ...................N...............i............................................ .............................................N.................................. ..........N...........N.........................................i............... ..............i..............................N.............................N.... .........................N.........................N............................ ......................................i....N.......N.........i.................. ......N..............................................i.......................... ...........................N.................................................... .......................N........................................................ .........N...................................................................... .............i....................................i............................. ......N..........N..........................N................................... ........................N..N.......i..................N......................... .............................................i.........i........................ .....................N.......................................................... ..................N.....N....................................................... ...................................................N....................N....... ........................................................N...................i... ..................N................N..................N......................... .............................................i.........N..N..i.................N ................................................................................ ................i....................N.......................N.......N.......... .............N.................................................................N .......N.......N..............................................i................. ................................................................................ .........................................N.......N.............................. ........................................N........ Pos Score Pred ------------------------ 20 0.399 - 36 0.536 Yes 126 0.499 - 171 0.211 - 183 0.302 - 225 0.651 Yes 255 0.621 Yes 286 0.164 - 316 0.061 - 346 0.167 - 372 0.268 - 439 0.524 Yes 444 0.497 - 452 0.471 - 462 0.563 Yes 487 0.135 - 534 0.668 Yes 588 0.412 - 664 0.121 - 730 0.142 - 814 0.538 Yes 851 0.604 Yes 887 0.250 - 898 0.257 - 925 0.079 - 985 0.322 - 988 0.234 - 996 0.552 Yes 1015 0.141 - 1086 0.546 Yes 1096 0.619 Yes 1142 0.424 - 1219 0.156 - 1225 0.251 - 1332 0.422 - 1353 0.334 - 1417 0.236 - 1437 0.535 Yes 1459 0.394 - 1476 0.364 - 1495 0.263 - 1566 0.780 Yes 1576 0.171 - 1579 0.420 - 1582 0.633 Yes 1600 0.327 - 1697 0.510 Yes 1718 0.448 - 1742 0.417 - 1750 0.268 - 1774 0.223 - 1840 0.323 - 1848 0.353 - 1856 0.212 - 1903 0.550 Yes 2042 0.096 - 2050 0.193 - 2121 0.052 -

Explain the output -- Processing locus 140: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGGAAGATGAAGAAACAAGGTTGGGTTTAACTCGTACCTTTCCCAATTAAAACCGCTTTGATTTTTAAATCAAAACATT GAACCGGGTTTAAATATATGAATGAATCAAAACCGGAATTTTCATTTCCTTCTTCTTCTCTTCTCTCTCACACAGCACAA AGGACCAACGATTCATTCATTGAAGAAGATGATACAGAGATTGATTCCTCTAAATCGAAAAGCTTCGAACTTTACTCAGA TTCTTGAGAAAGGTACTTCTTTATTACACTATTCTAGTATCACCGAAGCGAAGCTTTCTTATAAAGAGAGATTAAGGAAT GGGATTGTTGATATTAAGGTTAATGATGCTATTGATCTCTTCGAATCCATGATTCAATCTCGTCCTTTACCTACTCCAAT CGATTTCAATAGATTGTGTAGTGCTGTTGCTAGAACGAAACAGTATGATTTAGTGTTAGGTTTCTGCAAGGGAATGGAGT TGAATGGGATTGAGCATGACATGTATACTATGACCATTATGATCAATTGCTATTGTAGAAAAAAGAAACTTCTTTTCGCA TTTTCTGTTCTTGGGAGAGCTTGGAAGCTTGGGTATGAGCCTGATACAATCACGTTTTCGACTTTGGTTAACGGGTTTTG CCTCGAGGGTAGAGTTTCTGAAGCGGTTGCGTTAGTTGATCGGATGGTGGAAATGAAGCAAAGACCGGATCTTGTAACCG TTAGCACTCTGATCAATGGACTTTGTCTAAAGGGTAGAGTGTCTGAAGCTTTGGTTTTGATTGATAGGATGGTTGAGTAT GGTTTTCAACCTGATGAGGTTACTTATGGACCGGTTTTGAATAGACTGTGTAAGTCAGGGAACTCTGCTTTGGCTTTGGA TTTGTTTAGAAAGATGGAAGAAAGAAATATCAAAGCTAGTGTTGTTCAGTATAGTATTGTTATTGATAGTCTTTGTAAAG ATGGGAGCTTTGACGATGCGCTTAGCCTTTTCAATGAGATGGAGATGAAAGGGATTAAAGCTGATGTTGTTACGTATAGT TCTCTTATAGGAGGTCTTTGTAACGACGGTAAATGGGATGATGGTGCAAAGATGTTGAGGGAGATGATTGGAAGGAATAT CATTCCCGATGTTGTCACATTTAGTGCTTTGATTGATGTTTTTGTGAAAGAGGGGAAGCTTCTAGAGGCTAAAGAGTTGT ACAATGAGATGATCACAAGAGGTATAGCTCCTGACACCATTACATATAATTCATTAATTGATGGGTTTTGTAAGGAGAAT TGCTTACATGAGGCTAATCAGATGTTTGATCTGATGGTTAGCAAAGGGTGCGAACCTGACATCGTGACTTATAGTATTCT CATAAATAGCTACTGCAAAGCTAAGAGAGTTGATGATGGTATGAGACTTTTTCGTGAGATTTCTTCGAAAGGATTGATTC CTAATACAATCACTTATAACACTCTTGTCCTAGGGTTTTGTCAATCGGGAAAACTTAACGCCGCCAAAGAACTCTTCCAA GAGATGGTTTCTCGTGGTGTGCCACCAAGTGTTGTAACTTATGGTATTTTGCTGGATGGTTTGTGTGACAATGGTGAACT AAATAAAGCATTGGAGATATTTGAGAAAATGCAGAAGAGTAGGATGACTCTTGGTATTGGTATCTATAATATCATCATTC ACGGGATGTGCAATGCTAGTAAGGTCGATGATGCTTGGAGTTTATTCTGTAGCCTCTCTGACAAAGGAGTGAAACCTGAT GTCGTGACATACAATGTAATGATTGGAGGACTATGTAAGAAAGGCTCGTTGTCTGAAGCCGATATGCTGTTTAGAAAAAT GAAGGAGGATGGGTGTACTCCGGATGATTTTACGTACAACATTCTTATCAGGGCACATCTTGGAGGTAGTGGTCTAATCT CTTCAGTTGAACTCATAGAAGAAATGAAGGTGTGTGGGTTCTCTGCAGATTCTTCCACCATAAAGATGGTTATTGATATG TTATCAGATCGTAGATTGGATAAAAGCTTTTTGGATATGCTTTCTTGATTGTCTAATCTGATCTACAAGAGATAAAGAAA ATATTTTTGTTTGTAATTCCAAATCACTAAAATATTGTACCAAGCTTGGGGCTATTTATAAAGTAGA .......N........................................................................ .................i...N.......................................................... ............................N................................................... ..............................................................................N. ......................N..N......................N............................... ............................................N............................N...... ...N...........N....N........N........N......................................... ..................................N............................................. ...........................................i........N........................... ...............N....................................................i.........N. .............N...........N...................................................... .............i.................................................................. N..............N.................N....i.....i..................N................ ................................i....N..N..........i...........i................ ........N..........................N............................................ ...N....i...................................................N................... .......N.............i...........i.............................................. ................................N..N....i....................................... ................................................................................ ...i....................................N..............N..............N......... ............................i..............i.................................... .....N......N..............N..N...............................................N. .............N....i.............i..............................N..............i. ........N..............N........................................................ .......................N.........................................N...........N.. ....................................N........................................... ................................................................... Pos Score Pred ------------------------ 8 0.375 - 98 0.588 Yes 102 0.241 - 189 0.190 - 319 0.248 - 343 0.118 - 346 0.202 - 369 0.454 - 445 0.088 - 474 0.308 - 484 0.149 - 496 0.319 - 501 0.497 - 510 0.277 - 519 0.128 - 595 0.152 - 684 0.607 Yes 693 0.242 - 736 0.182 - 789 0.503 Yes 799 0.202 - 814 0.051 - 826 0.085 - 894 0.573 Yes 961 0.256 - 976 0.326 - 994 0.081 - 999 0.697 Yes 1005 0.714 Yes 1024 0.130 - 1073 0.527 Yes 1078 0.064 - 1081 0.122 - 1092 0.795 Yes 1104 0.544 Yes 1129 0.136 - 1156 0.083 - 1204 0.170 - 1209 0.514 Yes 1261 0.190 - 1288 0.191 - 1302 0.818 Yes 1314 0.652 Yes 1393 0.154 - 1396 0.304 - 1401 0.539 Yes 1524 0.621 Yes 1561 0.211 - 1576 0.107 - 1591 0.266 - 1629 0.720 Yes 1644 0.739 Yes 1686 0.404 - 1693 0.158 - 1708 0.175 - 1711 0.327 - 1759 0.118 - 1774 0.147 - 1779 0.571 Yes 1793 0.580 Yes 1824 0.355 - 1839 0.709 Yes 1849 0.371 - 1864 0.140 - 1944 0.417 - 1986 0.169 - 1998 0.109 - 2037 0.210 -

Explain the output -- Processing locus 141: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGTGTCAATATGTTCGTTTGTAATGAATGTATCAAGCAATATTTATCATAATTAAACTAGCTTGAAATGTAGTATTAC GATGTTGTTGTGTTCGTCTGATTTCTCTCACCGGTTGATGCATGGTTACGTTCGGCTCGGTTTTTATATGGTTCAATTTT TAATATATCGATAATTTTAAATGATTTCACTTGTATTATTAACAAAGCCCATTGGTATTTGGCCCAAAATCTGAATGCCG ACTTAATGACTTGTTTCTTCTTCCTTCAGCTTCTCCAACGAACGGCTTTTGTTTTAGTTTTTTTTGCTTACCTTTCTCTC GACTTGGTCTACTTTTGTTACTCTTTGAGTTGGGTGATTATGGCAGCAGCACCACAGGAGGAACGATCTCCGTCGCTTGA CGATTGCCTTAAGCTATTGAAAGGGGAGAGAGACGAGCAGAGGCTCGCCGGTCTACTTCTCGTCACCAAATTCTGTAAGA ACGATGACATCGTTTCTCTCAACAAGGTCTACGAAGCCGTTGGTACTCACTTTCTCGACCGTCTTTTCCGTACTGGTAGT GGCAGTGGCGATGGGGTTGATAATCGTGATGTGTATCTGCAGATATCTATCACTGTTCTTGCTGCATTCTGCCGTGTTCC GGAGATAGCTTCTTCAGAAGAGATGGTCTCCAGAATTCCCCTTATTCTCGAAGTAATGTCCAAAGGAATAACTACAAACA TGCTCGAAGATTGCTATGAGCTTCTGTATTTGGTGTCCACGGCTTGTGAAGCTGGTCTCATGACTTTGATTAACTCTGGA GGCTTGAGAGTGATAGCTCCCCAGATGTCTGAGTTACCTGATGGTTCACATGCCATGGAGGTTGCTATAAAGATTCTGCA GTTATTGGTTAGTAAGCTTGATTGTGGGAGCATGACTATTGAACGCTTCTTAGAACTGTCTTTGGTGATTGCTGCTGTTG CACGGCAGTTTGCGGTGTTGCATAATGCCCTGAAGTTTGAAGCACTCCATTTACTGTCTGCAGTCTTCTCGTCAGAGTAT TCTGAACTTCTTCATGAACCTCTTCGTTCCATGCCCGACAACAATTGGGCAGACTATATGCGCACAGGTGTTGTGGCAAT TCTGCAGAACCGTGTGGCACCTTCCGAAAAGCTTCATGCGTTGATTCTGGCTGAGAATATGATGTCAATATTGGGTGAAA AGTGGCTTCTCGGCGGGGTAAAATTACCCAATGTTGATATTCCAGCTGATAGGTGTCTTCTGTTGGTATTAGAGTCATCG CATGTTGAAATTTCTGTATTGTTAAATGATCTCGCTTACAAGAAGTATGAAGCTCCTAAAGATAATTCAACTGCTGAAGA CATTCTCTTGAAGCAACGGTATCTACCCATCGTGTTTTCACTGGTAGAAAAGATCATCAAATATATATCATCTGCTGGTG AAAACGAAGGGACTCTATCTGACGAAGCTGTCTTCCAAAAGGTGATTAAAATACTTAATGAGACAGTAGGAGCGGTGTTC GAATATCTCAGAGATGCAAAGGAACATGGAATTAAGAAAGGAACTGACCTTCTTGCATCTGTGCGAGTGATTGGAAGCTA TCTTGCGGAAACTCCTGTTGCGTGCAAAGAGCAGGTCCAGGATCTTCTGGATTATATGCTTTCAGTGAAGGGCGAAGATG AATCCAGCCCGTTTTTGTCTACTTGCTTCTTGCTCCCAATGCTTTGCCAAATAACAATGAACGCAGAAGGATGTAAACTC TTGGCCTATTCAAGAGGCGATGTAGCTGTCGTTGAATGTCTAATAAAATTGATTGAGCGCTGTGGTGAGAGTGTTGATGA GGATCGAAGCATATTCTTGGCTTGTGATACAATCATGAATATTCTTTTGAAGAGGGAAGAAATAAGCCCGCCGGAAATTT CAACATTTGCCAGTCTATTGAAAGCTTTGGCATATTGGGCAGATGGGAGTAATGACCATTCGGTAGTAATGATGGCTGCA AGCATATGCTCTCTGATCTTTGATTTCACATCAGAAGATGCTTTACAACTTAGCAGAAGCTCCCTGGATAGTTTGGCCCG GCTCGTTACAAGAAGCTTGTCCTCAAGTGGCCAGGATATGTCTGATACCGCGGATCTTCTTGAAATCATAAGAGCAGGAT ACTCTCGGTGGATAAATCGATTTCCCACCATTATGAAACATCCCGTTGTGCAATAGAAACTATTAGATTTGGTCTCAGGC TGTTTCGAGGGAGAGAAATAGTCAAAAAAGGAAATTTTGTTCAGTTTATTAGCTCCACGTTGTTGTATTATATAC ...........N............N...N.......................................N........... ....................N.....................................................N..... .....N.......................................................................... .......................................i........................................ ................................................................................ ...i............................................................................ ..........N.................N................................................... ......................i................................i.......................i ...............N...........................................N.................... ........................i...............N........N....i......................... ...............................N................................................ ........................N....................................................... .............N................N..........................N...................... ...................................N......................N..N.................. ..............................N................................................. ................................................................................ .........................................................N...................... .............N...........N...................................................... .......................................................N.....................N.. ......................................N.................i.............N......... ...................N...............N........................................N... ..................................N............................................. ..........................................N........N................i..i........ .....N...............................i.......................................... .....................................i.......................................... ................................N............................................... ........................................................................... Pos Score Pred ------------------------ 12 0.495 - 25 0.451 - 29 0.296 - 69 0.387 - 82 0.144 - 118 0.246 - 122 0.184 - 148 0.104 - 181 0.365 - 235 0.203 - 246 0.186 - 360 0.782 Yes 484 0.520 Yes 571 0.268 - 589 0.097 - 663 0.652 Yes 696 0.558 Yes 720 0.858 Yes 736 0.196 - 780 0.431 - 825 0.624 Yes 841 0.108 - 850 0.082 - 855 0.541 Yes 912 0.402 - 985 0.234 - 1054 0.183 - 1071 0.476 - 1098 0.379 - 1156 0.173 - 1179 0.482 - 1182 0.477 - 1231 0.319 - 1282 0.071 - 1306 0.186 - 1327 0.218 - 1498 0.205 - 1534 0.209 - 1546 0.270 - 1656 0.317 - 1678 0.481 - 1719 0.454 - 1737 0.605 Yes 1751 0.239 - 1780 0.116 - 1796 0.200 - 1837 0.182 - 1875 0.289 - 1963 0.294 - 1972 0.273 - 1989 0.562 Yes 1992 0.583 Yes 2006 0.363 - 2038 0.573 Yes 2118 0.619 Yes 2193 0.262 -

Explain the output -- Processing locus 142: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGAGGAGAAGATTAGAGAAAAGAAAGACAAAAAGTAGAAATGTTTTGCTCTGTTCTTTTGAGTTTTTGCTCTGTTTTGA CATTATGGTCTTGTTTTTTAATTTTTAATGAAACGTTGGAAAAAAAAAAAAAAAAACGAATTTCTTCTTTTGGGGTTTTG TTTTCTTTTAGGGTTTTTCATTTTTGTTAGCGAGAGGATTTTCTCGACGCGAGCTCCGGCAATGAAAAGTTTATCGATTT CTCTAATCCGTCGGCTTCATTTCCACAAAATCAGACCAATCCCACTTTCAATCCACCTTAATGCTGCTTCTTCATCGTCT TCGTCCTCTTCTCTGATCTCGCCAGCACGCGAAATCTCCACTAGCTGCAGAAGAACCAATGATCTGATCAAGCGTCAGTT CTGGGTCAATCCATATCGGAATTTGAATACCCATGTCGAGGAACCTCCGGAGATTTCGTCATCTGATAAAGAAAAAATCG ATCTCGAAGAAGCGTTCGAGTCAGCGAACACCACTGATGAAATGGTACGTTTGTTCAAGGAGATGGAGTTATCATTCGAA GGGAACGAATTAGGTTTATCTGCTTTGAAATTAGGGCTCCATCTCGATCGTGAAGGTGAAGATCCTGAAAAGGTTCTGTC TTACGCTGATAAAGCTCTTAAATCATTCGATGGAGATGGTAATAAACCTAATCTTCTCGTTGCTATGGCGTCGCAATTAA TGGGTTCTGCTAATTACGGTTTGAAACGTTTTAGTGATAGTTTAGGTTACTTGAATAGAGCTAATAGGATTCTTGTGAAG TTAGAGGCGGATGGTGATTGTGTTGTGGAAGACGTTAGGCCTGTTTTACACGCTGTGCAGCTTGAGCTTGCTAATGTTAA GAATGCTATGGGAAGGAGAGAGGAAGCTATTGAGAATTTGAAGAAGAGTTTGGAGATAAAGGAGATGACTTTTGATGAAG ATAGTAAAGAGATGGGTGTTGCGAATCGGAGTCTTGCTGATGCTTATGTTGCGGTTTTGAATTTTAATGAGGCTTTGCCT TATGCGTTGAAGGCGTTGGAGATTCATAAGAAGGAGTTGGGGAATAATTCAGCTGAGGTTGCGCAGGATCGGAGGCTACT CGGTGTGATTTATAGTGGTTTGGAGCAACATGACAAGGCGTTGGAGCAGAACCGATTGTCTCAGAGAGTGTTAAAGAATT GGGGGATGAAGTTGGAGCTGATTCGTGCGGAGATTGATGCTGCTAACATGAAGGTTGCTTTGGGTAAATACGAGGAAGCG ATTGATATATTGAAGAGCGTTGTTCAACAAACTGACAAGGACAGTGAGATGAGAGCTATGGTGTTTATATCAATGTCGAA AGCTTTGGTAAACCAACAGAAGTTTGCTGAATCAAAGAGATGTTTGGAGTTTGCTTGTGAAATCCTCGAGAAAAAAGAAA CAGCTTTGCCAGTTGAAGTCGCTGAAGCATACTCTGAGGTAGCAATGCAGTACGAGTCAATGAACGAATTTGAAACCGCC ATCTCTTTATTGCAGAAAACTTTAGGTATACTAGAGAAGCTTCCTCAAGAACAGCACTCAGAAGGAAGTGTTTCTGCTAG GATAGGTTGGTTGCTTCTATTCTCAGGCCGAGTCTCACAAGCGGTTCCTTACTTGGAAAGTGCAGCCGAGAGGTTAAAAG AAAGCTTTGGTGCTAAACATTTTGGAGTTGGATATGTATATAACAATCTCGGTGCTGCTTATTTAGAACTCGGAAGACCT CAATCCGCTGCTCAAATGTTTGCAGTTGCTAAAGACATTATGGATGTTTCACTTGGTCCAAACCATGTGGATTCCATCGA TGCTTGCCAGAATCTTTCTAAAGCTTACGCTGGAATGGGAAATTATAGCCTCGCAGTGGAATTTCAACAGCGAGTAATCA ATGCTTGGGATAATCATGGAGATAGCGCGAAAGACGAAATGAAAGAAGCGAAAAGACTTCTTGAGGATTTGCGATTGAAG GCTCGAGGAGGTGTATCAACGAATAAGCTTCTGAATAAGGCTTTACCTTTGCCCAAGCCAAGCCATTCATCTTAGTGAGC TTTGTGAAGTCTCAGCCCCAAAGAACAATGAAAAGAATAGTGAGATTTAATGTTTTTTCTTTTCAAATGATCGTCGTATA CTAAACTCTTGTTT ........................................i....................................... ....N......................N.................................................... .............................................................N.................. ............................................................i................... ..........................................................i..................... ................................N............................................... ....................................N....N....................N................. ................................................................................ .............................N.....N............................i..............N ................................................................................ ..........N..............................................................N...... ..N....i........................................................i.........N..... ...........i...........................N.....N....................N............. .............................N.................................................. .....i..............................N..........i................................ ................................................i........i..............N....... .......................................N........................................ ............................................N..............i.................... ................................................................................ ................................................................................ .................................N.............................................. ...............i.......................i...N....................N..............N ..................................i............................................. N..............N......................i......................................... ................................................................................ ...........................N.....................N................N............. .............. Pos Score Pred ------------------------ 41 0.519 Yes 85 0.085 - 108 0.103 - 222 0.250 - 301 0.638 Yes 379 0.584 Yes 433 0.221 - 517 0.347 - 522 0.448 - 543 0.432 - 670 0.266 - 676 0.136 - 705 0.704 Yes 720 0.434 - 811 0.088 - 874 0.065 - 883 0.100 - 888 0.620 Yes 945 0.759 Yes 955 0.088 - 972 0.689 Yes 1000 0.057 - 1006 0.070 - 1027 0.075 - 1042 0.079 - 1150 0.136 - 1206 0.758 Yes 1237 0.110 - 1248 0.587 Yes 1329 0.554 Yes 1338 0.581 Yes 1353 0.473 - 1400 0.280 - 1485 0.405 - 1500 0.616 Yes 1714 0.236 - 1776 0.759 Yes 1800 0.861 Yes 1804 0.219 - 1825 0.397 - 1840 0.271 - 1875 0.902 Yes 1921 0.439 - 1936 0.249 - 1959 0.669 Yes 2108 0.096 - 2130 0.052 - 2147 0.070 -

Explain the output -- Processing locus 143: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTTTAGTATTTTGATCATAGAATGCTTGCCGTTTCGTTAAAGCGCTAAACCTCCTCCTCATCCTCTCCCAGCTAGCTCC TCTTGCGGTTTCAGAGCTTAAACCCCAATCTCCCTCAAAACTCATAAACATATTGCTCTCCTTTGTTCATTTCTGGGTTT ACGCTTCTTTCGACTTTATGGGTTTCGGAGCTTAGTTTTACTACAATTTCGTTTGGAAAAGAGCTCTTATTTGATGTTCA TGGCGTCTCTCTCCTTCACCCAATTCTTATCATTCCCCAGATGCAATGCAGATGTTCCTTGTCTGCTCCAGTCGCATGGT TTTGTGAAGTTTAGAGGTGAAAGATGGAATGGGAAACAAAGCTTTTCTATGGCTGCTGGTAGGAGAAAGTTATCAGAGTC AGCACCTCTTGAAGAAGAAGGTAATGATGGAAACGGTGCGGTTGTGGGAAAGAAACCATCTAAAAGTGTTAAGAGAACAA CGAAGAAGAAGGTTGTGGTTAAGGATGAACCATTAGAAGAAATTTCTGAATTTTTGGTTGATAATGATGATGTTTTAGAC AAGGAAAGCATTGTCTCAGCTTTGAAACCAAAAAAGACAAGAACTAGAAAGAAAGCTGCAGCAGCTTCTTCTGATGTGGA GGAAGTAAAAACCGAGAAGAAAGTGAGACGAAAGAGGACTGTTAAGAAAGATAAAGATGTGGAGGATGATTTAGCTACTA TTATGGATGCTGAGGTTAGTGATGTCGAGGAAGCATTGGCTGTTGAAAGCACTGATACAGAGAGTGAAGAAGAAGAGATT GATCTGAGTAAACATGAAGGTGAAGATATTAGTCACACTTATGGATGGCCTCCTCTCGTGTGTTGCTTTGGATCTGCACA ACATGCTTTTGTGCCTTCGGGAAGGCCAGCAAACAGGCTATTAGATTATGAACTACACGAACGGATGAGAGATGCAAAGT GGGCTCCAGAGAAGTATATCCGGGCTCCTGGAGGATGTGCAGGAGGTGTTGCTATTGCTCTTGCAAGCTTGGGTGGTAAG GTTGCTTTTATGGGCAAACTTGGGGCTGATGACTATGGTCAAGCCATGTTGTATTATCTGAATGTGTGCAAAGTCCAAAC TAGATCAGTTAAGATTGATGGTAAAAGGGTTACGGCGTGTTCTACTATGAAGATCAGCAAGAGAGGTCGCTTGAAATCGA CTTGTATTAAGCCATGTGCAGAGGATTCACTTTCAAAATCTGAAATCAACGTTGATGTGCTCAAAGAGGCGAAAATGTTC TACTTCAGCACGCATTCTCTACTCGATAAGAAGATGATGTCAACTACGATACAAGCCATCAAGATATCAAAGCAACTAGG CAATGTCATTTTTTATGACTTGAATCTCCCTTTACCACTATGGCACTCCAGCGAAGAAACCAAGTCTTTCATACAGGAAG TGTGGAATCTTGCAGACGTCATTGAAATCACAAAACAGGAACTTGAATTCTTATGCGGAATCGAGCCTACTGAAGAGTTC GATACAGAAAACAATGACATCAGTAAGTTTGTCCATTACCCGCCAGAAACTGTGGAACAGCTTTGGCATGAAAATCTCAA GGTCTTGTTTGTGACCAATGGCACTTCTAAGATCCATTACTATACCAAAGAGCACAATGGTGCTGTTTCCGGAATGGAGG ATGTTCCCATTACTCCTTTTACAAGGGATATGTCAGCATCTGGAGATGGCATCGTTGCAGGTCTAATAAGAATGCTTACG GTTCAGCCAGATCTTATGAACAATAAAGGGTACCTGGAACGCACAGCGAGATATGCAATTGAGTGTGGTATTATTGATCA ATGGTTGCTGGCACAAACCCGAGGATATCCTCCAAAGGATGACATGGAAGAAGAAGAAGATGATGAAGAAGAAGATGAAG TGGAATCAGATCCAAACGGTATAAGATCGATAACAGAGAAGGAATACAGAACCTCAAAGCCTTATGATGAACCAGATGGT CCATACGTTATGAAACCAGTAGAGGAAAGGGAGTACAAGAAGTTAGAGCTTGTTGGCTCAATGTTTGAAGATGGTAGTTT ATGATTTTCATAAGATTTGATAATGTTTCTGTTAGTCAGCTTTTTTTTCCTGTTCACATCTTACAACATCTTAGTAGTGT GAATTTTTGTCATATGTTTTGAT ......................i......................................................... ................................................................................ .................N.......................................................N.....N ........................................N....i.....N.......................i.... .......................i....i...................i............................... .......................N..N..................................................... ........................N......................................N..N..N.......... .........................................................................N...... ........................................................N........N.............. ..i...N..............N.......................................................... .............N..........................i...i................................... ..N............................................N................i......N........ ..................................N............................................. .........i..................N.....N..........i...............N.................. .................N............................i................................. .............N........................................N...................N..... .................................N..N........................................... ..N...........N........................N........................................ ....................................................N........................... .............N.....................................................N............ .................N......................................N................i...... N............................i...............N.........................i........ ...............N....................................N........................... N.....................................N....i...............N..i...........N..... ...............................................................N..N........N.... .........N..................................................N.........N......... N.....................N......................................................... .............N......... Pos Score Pred ------------------------ 23 0.510 Yes 178 0.422 - 234 0.315 - 240 0.408 - 281 0.269 - 286 0.615 Yes 292 0.395 - 316 0.521 Yes 344 0.519 Yes 349 0.602 Yes 369 0.707 Yes 424 0.094 - 427 0.204 - 505 0.157 - 544 0.197 - 547 0.220 - 550 0.181 - 634 0.049 - 697 0.407 - 706 0.173 - 723 0.825 Yes 727 0.072 - 742 0.135 - 814 0.326 - 841 0.730 Yes 845 0.583 Yes 883 0.186 - 928 0.429 - 945 0.748 Yes 952 0.256 - 995 0.374 - 1050 0.710 Yes 1069 0.139 - 1075 0.362 - 1086 0.606 Yes 1102 0.092 - 1138 0.224 - 1167 0.537 Yes 1214 0.203 - 1255 0.120 - 1275 0.273 - 1314 0.344 - 1317 0.479 - 1363 0.336 - 1375 0.439 - 1400 0.273 - 1493 0.230 - 1534 0.225 - 1588 0.315 - 1618 0.473 - 1657 0.324 - 1674 0.843 Yes 1681 0.104 - 1710 0.558 Yes 1726 0.145 - 1752 0.748 Yes 1776 0.404 - 1813 0.458 - 1841 0.472 - 1879 0.433 - 1884 0.760 Yes 1900 0.492 - 1903 0.595 Yes 1915 0.281 - 1984 0.072 - 1987 0.160 - 1996 0.134 - 2010 0.187 - 2061 0.183 - 2071 0.196 - 2081 0.139 - 2103 0.124 - 2174 0.052 -

Explain the output -- Processing locus 144: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGGAAACAAAAAAAAAAGGGTGATGATGGATGGACCATTTTGGCTTATGTTTTTATTGCTCAATAACAGTGACATGTG TTTATGTGTGTTATGATTTAAAAGTTTTGTTTTTTTTTGCTGATGGATTTGTTTTTTTTCTTTTTTTTTGTTAATGGCTT TTGCAGCTTTGCGGATCTTCAGAAGCCTCAGACTGAACTTGATGGGAAGGCTTGTGCTGGTGTTGGACAAAGCAGTCTTA TGGCTTACTATGAGACTATGTTTGACCAGCTTGATGTGACGGCAGCTCAACTTCTGGTGAATGACAGTAGTTTTAGAGAC AAGGATTTCAGGAAGCAACTTAATGAAACTGTCAAGTCTATGCTTGATTTGAGGGTTATTCCAATTTTCAATGAGAATGA TGCTATTAGCACCCGAAGAGCCCCATATCAGGATTCTTCTGGTATTTTCTGGGATAACGATAGCTTAGCTGCTCTACTGG CGTTGGAACTGAAAGCTGATCTTCTGATTCTTCTGAGCGATGTTGAAGGTCTTTACACAGGCCCTCCAAGTGATCCTAAC TCAAAGTTGATCCACACTTTTGTTAAAGAAAAACATCAAGATGAGATTACATTCGGCGACAAATCAAGATTAGGGAGAGG GGGTATGACTGCAAAAGTCAAAGCTGCAGTCAATGCAGCTTATGCTGGGATTCCTGTCATCATAACCAGTGGGTATTCAG CTGAGAACATAGATAAAGTCCTCAGAGGACTACGTGTTGGAACCTTGTTTCATCAAGATGCTCGTTTATGGGCTCCGATC ACAGATTCTAATGCTCGTGACATGGCAGTTGCTGCGAGGGAAAGTTCCAGAAAGCTTCAGGCCTTATCTTCGGAAGACAG GAAAAAAATTCTGCTTGATATTGCCGATGCCCTTGAAGCAAATGTTACTACAATCAAAGCTGAGAATGAGTTAGATGTAG CTTCTGCACAAGAGGCTGGGTTGGAAGAGTCAATGGTGGCTCGCTTAGTTATGACACCTGGAAAGATCTCGAGCCTTGCA GCTTCAGTTCGTAAGCTAGCTGATATGGAAGATCCAATCGGCCGTGTTTTAAAGAAAACAGAGGTGGCAGATGGTCTTGT CTTAGAGAAGACCTCATCACCATTAGGCGTACTTCTGATTGTTTTTGAATCCCGACCTGATGCACTTGTACAGATAGCTT CACTTGCCATCCGTAGTGGAAATGGTCTTCTGCTGAAGGGTGGAAAGGAGGCCCGGCGATCAAATGCTATCTTACACAAG GTGATCACTGATGCAATTCCAGAGACTGTTGGGGGTAAACTCATTGGACTTGTGACTTCAAGAGAAGAGATTCCTGATTT GCTTAAGCTTGATGACGTTATCGATCTTGTGATCCCAAGAGGAAGCAACAAGCTTGTTACTCAGATAAAAAATACTACAA AAATCCCTGTGCTAGGTCATGCTGATGGAATCTGTCATGTATATGTCGACAAGGCTTGTGATACGGATATGGCAAAGCGC ATAGTTTCTGATGCAAAGTTGGACTATCCAGCAGCCTGTAATGCGATGGAAACCCTTCTTGTGCATAAGGATCTAGAGCA GAATGCTGTGCTTAATGAGCTTATTTTTGCTCTGCAGAGCAATGGAGTCACTTTGTATGGTGGACCAAGGGCAAGTAAGA TACTGAACATACCAGAAGCACGGTCATTCAACCATGAGTACTGTGCCAAGGCTTGCACTGTTGAAGTTGTAGAAGACGTT TATGGTGCTATAGATCACATTCACCGACATGGGAGTGCACACACAGACTGCATTGTGACAGAGGATCACGAAGTTGCAGA GCTATTCCTTCGCCAAGTGGATAGCGCTGCTGTGTTCCACAACGCCAGCACAAGATTCTCAGATGGTTTCCGATTTGGAC TTGGTGCAGAGGTGGGGGTAAGCACGGGCAGGATCCATGCTCGTGGTCCAGTCGGGGTCGAAGGATTACTTACAACGAGA TGGATAATGAGAGGAAAAGGACAAGTTGTCGACGGAGACAATGGAATTGTTTACACCCATCAGGACATTCCCATCCAAGC TTAAACAAGACTTCCGAGTGTGTGTTTGTGTATTTGGTTGAGACTTGAGGAGAGACACAGAGGAGGATGGGCTTTTTTGT TTCCTCTCTGCTTAGTACTCATA ........................N..N...N................N..........................N.... ...N........N.............................i..............................N...... .........................................N.....................................i .........N.......i...............N..........................N................... ......................N................i..............................N.....N..N ................................................................................ .......................................N........................................ ........................................N....................................... ....i...........................N........N...................................... .........................................................N.........N............ ..........N..........i.......................................................... ..........................N..............N.......................N........N..... ................................i.................i............................. ........................N.............................................N......... ...........................................................N.................... .....................N.........................................N................ ..........N..................................................................... ...........N.................................................................... ..................N.....N...........N.....N.........................i........... ..........N.............................N....i.................................. ..N...........N..........................i..............i....................... .................................N.............................................. ..............................................................i................. ....................................N..........................................N ......N.................................i....................................... ..................................................................N............. ....................... Pos Score Pred ------------------------ 25 0.232 - 28 0.317 - 32 0.353 - 49 0.062 - 76 0.129 - 84 0.246 - 93 0.323 - 123 0.545 Yes 154 0.229 - 202 0.151 - 240 0.795 Yes 250 0.108 - 258 0.716 Yes 274 0.153 - 301 0.091 - 343 0.068 - 360 0.572 Yes 391 0.147 - 397 0.301 - 400 0.219 - 520 0.305 - 601 0.322 - 645 0.760 Yes 673 0.093 - 682 0.126 - 778 0.249 - 788 0.330 - 811 0.241 - 822 0.741 Yes 907 0.225 - 922 0.096 - 946 0.137 - 955 0.158 - 993 0.681 Yes 1011 0.539 Yes 1065 0.456 - 1111 0.234 - 1180 0.183 - 1222 0.384 - 1264 0.131 - 1291 0.128 - 1372 0.076 - 1459 0.151 - 1465 0.406 - 1477 0.160 - 1483 0.105 - 1509 0.766 Yes 1531 0.076 - 1561 0.080 - 1566 0.721 Yes 1603 0.353 - 1615 0.109 - 1642 0.525 Yes 1657 0.634 Yes 1714 0.353 - 1762 0.198 - 1789 0.374 - 1903 0.552 Yes 1957 0.428 - 2000 0.442 - 2007 0.410 - 2041 0.691 Yes 2147 0.076 -

Explain the output -- Processing locus 145: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAACAAAACAAATTCTCATATACTAGAATTATTTTATTCTGTATATATAAACAATTCTAATATATTTTGGCTGAAAAA AAAAGCACCACGATATTGTAGTCTGGCAAACTAGTACCTGTGCCTATATATATATTTTTTTTCGTGCCTATATATTTGAT GGTGACAATTGATTCCATATCATTTGTAATGAACATGGAAGCCACATCAGTTTTTAGGCCAAAACTTGGATCTCAGTTTT CTCTTCCTTCTACGACCAATTTATTTCCGTTAAGAAAACTCTATGGCTTTCCTCTGACGTCATTTCCCGGTAAGCCTATA AAGCGTATTCGTTTGAAGGCCACTAATACTCTGACATTTGATGATAAAGAACGTACTCGCAAGTTCAAGAAATTACCACT TTCTGAATGGACTCACTACTTCCACTCGATTCCCCTTGATATCTCGGAAATGGATGCACTTAAAGAAGAGATCGATGAAC TAAAGCCGAAAGTGAAAAACACATTCATGTCTTCCCAAGGCAGTGACTCTACAAAGACGAAAATCCTTATGATATATTTG TTTGTGAGCCTTGGCCTTGCATATCACTTCGAGGAAGAGATATATGAGACTCTAAAAGAGGGTTTCGAAAATATAGAGAA GATCATGGCCGGTGAAGAAGATTTGTACACAGTTTCGATCATCTTTTGGGTTTTCAGAAGATATGGTCACTACATATCTT CAGATGTATTCCAAAGATTCAAAGGGAGCAATGGAAGTTTTAAAGAGTCTCTTATCGGAGACGCCAAGGGTATGTTGAGC TTGTATGAAGCTGCACATTTGGCGACAACAAAAGATTACATATTGGACGAAGCATTGATCTTTACATCGAGCCACCTGGA GACATTAGTTGCAACTGGAACGTGCCCACCTCATCTCTTAGCGCGTATACGAAATGCTCTTAGCATCTGTCAGCATTGGA ACTTTGAGGTATTAGTCCCACTGGATTTCATACCATTCTATGAACAAGAAAAAGACCATGACGAGATGCTACTCAAATTT GCTAAGCTCAGTTTCAAGTATCTGAAGCTCATATACCTTCAGGATCTCAAAATCCTTACAAAATGGTACAAGAAGCTAGA TTTTCCATCTAAGTTTCCACCTTACTTCAAAGACAGATGCGTCGAAAATTATTTCTTTGTGTTACCGGTGTTCTTCGAAC CACAACTCTCAAGTGCAAGGATGTTGTTGACTAAGGGCTTCATCCTTCTGGGGATTCAGGATGACACATTCGACAGATAT GCATCTATTTCTGAGGCCGAAAGCCTCGGCAACAGCTTGAAAAGGTGGGCTCCAGATCATTCCATGGACAAACAACCTGA ATATTTGAAATCCGTATTGAAGGTTATATTAGATACTTTCCAAGAGTTTGAAAAAGAACTTAGCCCCGAAGGAAGATCTT ACAGTGTGAAATACACAATTGAAGAGTTCCAGGCATCTTCGAAAGCTAACGTTGAGTTGGCAAAATGGGCACAAGTGTCT CACGTACCTAGCTTTGAGAAGTACATGGAAGTTGGACAGATGGAAATCACCGCATGTGTGACCGTTGCATATATTTTAAT GTCTATGGGAAAGACGGGTACTAAGGAAGCTTTTGAGTGGCTAAAATCTAGGCCAAAACTCGTCCAATCTTTATGTACTA AAGGTCGTCTTATGAATGATATAGCCGGTTTTGAGGATGACATGAGTAGAGGATATGTCGTGAATGCTGTCAATTGTTAT ATGAAGCAATATGGAGTTACTGAAAAGGAAGCTTTTAAGGAGCTTCGTAAAATGGTTGTCAATACACATAAGACATTAAA TGAGGAGTTCTTGACCACCACTTGCGTGTCACATTATGTTCTTAGGGAAACCATGGATTTTGCACGCATGATCATCGTCA CCTACAATGGATATGAAGGATTTACCCGTCCTGATGAAGGAAAAATCGAGGAGTATATGACTTCGTTATTCGTCGATCAT ACTCTGAAAATGTTGTAGTGTTTGATTCAGGAGTAGTGATGTTAATATGGGTATTCAAACCCTATTTAATTACTGGATAT GTGTAAAAGAAATTGACCCATTTAGGGAAAAAATTAA ................................................................................ ..............................................................................N. ............................N.....N............................................. ..........................................N..................................... ........................................N....................................... ......i..........................................N...N....................N..... ..........................i.........................................i........... ...........................................N.................................... ....i.........................................................N................. ...N..........................i........................................N........ ....N........................................................................... .....................................................N.......................... .......................................N.................N.......N.............. ..............................................................i................. ....................................i........................................... ....................i.......................................i.................N. ...............................................................N................ ................................................................................ ................................................................i............... ........................N..............i.............N........................N. ....N...................................................................N....... ...........i...N....................N....N............N........N................ N.........N........................................N...........................N ...................................N................i..............N............ ......N.....N....................N......................N....................... .........N............................N.......N...............................N. ..................................... Pos Score Pred ------------------------ 159 0.315 - 189 0.258 - 195 0.264 - 283 0.255 - 361 0.225 - 407 0.640 Yes 450 0.443 - 454 0.192 - 475 0.140 - 507 0.712 Yes 549 0.649 Yes 604 0.228 - 645 0.687 Yes 703 0.303 - 724 0.469 - 751 0.732 Yes 792 0.421 - 805 0.151 - 934 0.409 - 1000 0.210 - 1018 0.352 - 1026 0.389 - 1103 0.700 Yes 1157 0.725 Yes 1221 0.640 Yes 1261 0.500 - 1279 0.284 - 1344 0.443 - 1505 0.500 Yes 1545 0.368 - 1560 0.653 Yes 1574 0.467 - 1599 0.290 - 1605 0.474 - 1673 0.114 - 1692 0.542 Yes 1696 0.141 - 1717 0.380 - 1722 0.422 - 1735 0.142 - 1744 0.172 - 1761 0.332 - 1771 0.145 - 1812 0.491 - 1840 0.393 - 1876 0.417 - 1893 0.660 Yes 1908 0.311 - 1927 0.314 - 1933 0.175 - 1954 0.211 - 1977 0.355 - 2010 0.229 - 2039 0.055 - 2047 0.141 - 2079 0.042 -

Explain the output -- Processing locus 146: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGGCTTTGAAGTTGAGAGGCCCACAATTTTTGAAGGCTCAGACGTAAACGGTGACGTATAGGAGGCAAAACCCTTGCAA TTGCAGTGTCGTCGTCGTTGAGTAGTCGAAGACGGAGGGAAAGAAACAGAGAACTTGTTTGTTTCGTCGAAGAATAAGAT GATGAAGAGAGCCCAACAATCGGCAAGAGAAACAAAGCAAGAAGCTAAAGATGCTAGCAAAGCGAAGAGCGGTTTGTTCG CTTCTTGTTCCTTCTCTTCACTTGGTTTAGATACCAAGCTCTCTGACCAGCTCAAAGAAAGGATGGGTTTTGAAGCTCCT ACTCTTGTACAAGCTCAAGCAATTCCTGTTATTCTCTCTGGTCGAGATGTACTTGTTAATGCTCCAACCGGTACTGGAAA GACTATAGCGTATTTGGCACCCCTCATCCATCACTTGCAGGGTCATTCTCCTAAGGTTGATCGTTCTCATGGAACTTTTG CTTTGGTGATTGTGCCTACACGGGAACTATGCCTTCAGGTGTATGAAACCTTGGAGAAGTTACTGCATCGGTTCCATTGG ATAGTCCCTGGCTATGTTATGGGAGGAGAGAAAAAGGCTAAAGAAAAGGCTAGATTAAGGAAAGGAATATCCATTCTAAT TGCAACCCCGGGACGCCTTCTTGACCATTTGAAGAACACAGCTTCATTTGTGCACAAAAATCTGCGTTGGGTCATCTTTG ATGAAGCTGATAGCATTCTCGAATTAGGTTATGGGAAGGAGATAGAACAGATAATTAAGCTTCTAGGTTCAGGGCAAAAC GAGCAAGGGGAAGAAGATGATATTGTTCCAAAGGGTATTCAGAAGCAGAACTTGTTATTGTCAGCAACATTGAATGATAA AGTTAACGATCTTGCAAAACTTAGTTTGGACGATCCGGTAATGATTGGTCTTGACAACACCAAATTGCAACAAAATCTAT CAATAGAGTCTCCTGCGGCTCCTGACTCTGATGCGGAAGATATGGTAATTCATGTTAACAAATCCGCAAACCCTTTATCT GAGGACTATGGAATACCATCACAGCTGGTACAGAGATATCTTAGAGTGCCATGTGGTGCACGGCTTGTGGCACTTCTTTC TGTTCTCAAGAACCTTTTTGAAAGAGAAGCTTCTCAAAAGGTGGTCGTCTTCTTTTCGACACGTGATGCTGTAGATTTTC ATTACTCACTTCTGAGTGAATTCCAGTGGCCACCGAATTCTGAGACAGAAGAGGAGGGGACTAAAGAGTTGTTTCTCAAG TGCAAAACGTTCCGGTTACATGGAAGTATGGAGCAGGAGGATAGAAGATCTGCGTTTGGGACCTTTAAAACAGAGAAGCA GGCTGTTCTGTTGAGTACAGATGTTGCTGCTAGAGGCTTGGATTTCCCAAAAGTAAGATGTATTATACAATATGACTGTC CCGGGGAAGCTACCGAGTATGTGCATAGAGTTGGTAGAACAGCTCGTATTGGTGAAAAAGGAGAAGCCTTGTTATTTCTA CAACCGATTGAAATAGACTATCTTAAGGAGCTGAAGAAACATGGTGCATCACTTACAGAGTACCCTCTTATGAAAGTACT TGATAAATTTCCCATACCCGGCAATATGCCCCGAATCAAAAAGGTCCTATCTCTAGAATCCCATCCATGGGTCATATCTC TGCAGAGAGCTCTTGAATCCCTTACTTACGCCGAGCCAAAGATGAAGAGCCTGGCCAAGAATGCATTTGTCTCTTGGGTT AGGGGATATGCAGCTCACAAGGGAGAGCTCAAGAGTATTTTCGTGGTGAAGAAGCTTCACTTGGGTCATGTCGCCAAGAG TTTTGCTCTGAGAGAACAACCGTCTTTAGTTGGGAAATCACATCACAAGGAAACAATGAAGAGAAAGAGAGACGAACGTC AAAAAGGGCAACAAGGTAAGAAGAGAAAGAAGATGAGTGGTACTGGCAATAGAAGTACACAAAAAACTTGATCAAATCGG TTTCGCCACCATAAACCGTGGCGTAAGTTTTGTAGTTAAGGAGCTGGTTTGCTCATCGAATATTTTGAAATATCGAAAAG AAGTTGGGAG ................................................................................ ..............................................................................N. ..............................................................i................. ..............................................N...........N..................... ....................................................................N........... ............................i.............N..................................... .............N....N............................................................. ................................................................................ N.............................N................................................. ................N........................................................N...... ........................................i....................................... ..............................N..........i.........N............................ .......N..........................................N............................. .................................................................N.............. ................................................................................ ...................N.......i.................................................... ....................N....................................N.............i........ ..................N............................................................. ........................................N............................N.......... .........................i........................................i............. .........................................N..................N................... .......N...........................................................N............ .......................................................i........................ ................................i............................................... ................................................................................ .......... Pos Score Pred ------------------------ 159 0.459 - 162 0.241 - 211 0.552 Yes 303 0.885 Yes 367 0.129 - 379 0.086 - 469 0.190 - 509 0.805 Yes 523 0.166 - 574 0.286 - 579 0.455 - 721 0.245 - 751 0.175 - 817 0.347 - 874 0.152 - 921 0.584 Yes 991 0.137 - 1002 0.622 Yes 1012 0.329 - 1048 0.263 - 1091 0.368 - 1186 0.323 - 1300 0.168 - 1308 0.819 Yes 1381 0.439 - 1418 0.397 - 1432 0.514 Yes 1459 0.205 - 1561 0.490 - 1590 0.302 - 1626 0.660 Yes 1667 0.668 Yes 1722 0.454 - 1741 0.294 - 1768 0.301 - 1828 0.393 - 1896 0.653 Yes 1953 0.525 Yes

Explain the output -- Processing locus 147: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAGAAAACATAGTACTACTACTTAATTGTCTAATTTGCTTACTAATTAATTAGTTTATTGACTAATCAGAAAAAGTAAC ATGTTTGAAGTTAGTGTTTAAGAATTAATACAAATGTTGCGTCATCATCTCTCGCTGTCTTGTCAAATCTGTAACTGCAT ATTTATTTATAAATCTCTCTTCTCTTTACTCATTCTCTTCTCTCTCACTTTCTCAAACAATCTCTCTCTCTGGCATTTGG ATTTGGAAGTGCTTTTTGTATATATATCCAACAAAGGAATCAGATCGGAGATGTCGGATGTTTCCGGTGACGGAGATTTA TCGGCGACGGTTACTGAACATGAAGTAACTCCACAACCGCCGGTTTCTTCGGCGACGTATCCGTCATTAACGGTGTCGGC GTCTTACAAAGAGAGCAGCGGAGGTAAAAGCTCGTCGAAGAGGAGACCAATAAGGCCGAGTTTCGACGCTGCGGCTGATA ATGAGTTTATTACTTTGCTTCATGGCTCGGATCCAGTGAAAGTGGAGCTTAATCGACTAGAGAATGAAGTTAGAGATAAG GATCGGGAGTTAGGAGAAGCAAACGCTGAGATCAAAGCGCTTAGGTTGTCTGAGAGACAAAGAGAGAAAGCTGTTGAAGA GCTTACTGAGGAGCTTACTAAGTTGGATGAGAAGCTCAAGTTGACTGAATCCATTCTCGAAAGCAAAAATCTAGAAATCA AGAAAATCAATGAGGAAAAGAAAGCATCAATGGCTGCTCAGTTTGCTGCTGAAGCCACTTTAAGAAGAGTCCATGCTGCT CAAAAGGATGATGACATGCCTCCTATTGAAGCCATCCTTGCTCCATTAGAAGCTGAACTTAAGCTTGCACGATCAGAGAT TGGAAAGCTTCAAGAAGATAACAGGGCTTTGGACCGTCTGACTAAATCAAAGGAAGCTGCCTTACTTGAAGCTGAGAGGA CCGTTGAAGCTGCCATGGCAAAAGCCGCTATGGTTGATGATCTTCAGAATAAGAACCAGGAGCTAATGAAGCAAATTGAA ATCTGTCAGGAAGAAAACAAAATTCTAGATAGAATGCATAGGCAGAAGGTTGCTGAAGTGGAAAAGCTTACTCAGACAGT ACGAGAGCTAGAAGAGGCTGTTCTTGCAGGTGGTGCTGCTGCAAATGCCGTGAGGGATTACCAGCGGAAATTTCAAGAGA TGAATGAAGAGCGGAAAACTCTTGACCGGGAGCTTGCACGTGCAAAGGTTACAGCAAACAGAGTTGCCACTGTGGTAGCA AATGAGTGGAAAGATGGTAATGATAAAGTGATGCCTGTGAAGCAATGGCTCGAAGAACGAAGGTTTCTACAGGGAGAAAT GCAGCAACTTCGTGATAAGCTCGCCATAACAGACCGGGCTGCAAAATCCGAGGCACAGTTAAAAGAGAAGTTTCAACTGC GGCTTAAAGTGCTTGAGGAGACACTTCGAGGAACTTCAAGCAGCGCCACCAGGAACACACCCGAGGCAAGAAGCATGAGT AATGGACCTTCTCGCAGGCAGTCGCTTGGTGGAGCTGAAAACTTACAAAAATTTACATCAAACGGTGCTTTATCAAAGAA AGCTCCAGCATCTCAGATGAGGCATTCATTGTCTATTAATTCAACTTCGGTGTTGAAGAATGCTAAAGGAACATCTAAGT CATTTGATGGAGGCACAAGATCCGTGGATAGAGGCAAAGCACTTTTAAACGGACCTGGGAATTATTCCTTCAACAAGGCT ACTGATGACTCTAAGGAAGCAGAGTCAGGTAATGGGTGGAAAGAAAATTCAGAGGAGAAGCCACAAAGTGAAGACCCTGA AGCAGCAACTGAGGACAGTGTCCCTGGTGTTTTGTATGATTTACTACAGAAAGAAGTAGTTTCTTTGAGGAAAGCCTCTA ATGAAAAGGATCAAAGCCTCAAAGACAAAGATGATGCCATCGAGATGCTAGCGAAGAAGGTGGAAACATTAACAAAGGCA ATGGAGGTTGAAGCAAAAGAAGATGAGACGGGAAGTAGCTGCAATGGAAAAAGAAGTTGCTGCAATGCGTGTGGAGAAAG ATCAGGACGCCAGAGCCAAAAGGTTTTCAAATAGCAAGAGCCCATCCAACACCGCCCAGATTCTTGCGGGAAGGTAGAGC TATGTGGTTTTTCACTTTGTATTACACATTTCATGGTTAGCTATAATGCTATA ................................................................................ N................................N.............................................. ................................................................................ ..................................................i......N...................... ...................N............................................................ ................................................................................ N....................N.........................................N................ ................................................................................ ..........................N..................................................... .........N...................i..........................................N....... .......N..N....i................................................................ ................................................................................ ..............i..............i......N............................N.............. .................................i.............................................. ............................................N..................................i ...N............................................................................ ................................................................................ ..........................................................................i..... ................i..........................................i.................... ......N......................................................................... ....N..........................N................................................ ...................................N............................................ N.............................N..N..........i................................... i.....................i....................i....................i............... ................................................................................ Pos Score Pred ------------------------ 81 0.219 - 114 0.210 - 291 0.689 Yes 298 0.335 - 340 0.226 - 481 0.279 - 502 0.235 - 544 0.251 - 667 0.089 - 730 0.492 - 750 0.904 Yes 793 0.303 - 808 0.165 - 811 0.158 - 816 0.869 Yes 975 0.612 Yes 990 0.513 Yes 997 0.082 - 1026 0.471 - 1074 0.827 Yes 1165 0.199 - 1200 0.553 Yes 1204 0.174 - 1282 0.260 - 1294 0.460 - 1300 0.151 - 1311 0.605 Yes 1325 0.783 Yes 1359 0.510 Yes 1515 0.501 Yes 1522 0.654 Yes 1617 0.660 Yes 1660 0.578 Yes 1687 0.477 - 1765 0.388 - 1792 0.479 - 1876 0.110 - 1921 0.241 - 1951 0.490 - 1954 0.303 - 1965 0.663 Yes 2001 0.731 Yes 2023 0.560 Yes 2044 0.644 Yes 2065 0.510 Yes 2162 0.086 - 2193 0.052 - 2206 0.050 -

Explain the output -- Processing locus 148: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCACAGCCAAATTCACACCATTCTTTATTTTCATTTTTCCCCCTTTGCCCAGATTCCGACAAAATCCACTCGTTTGAT TCCACCACTATATATAATTGAGCGGAGAAGCTTGACATGAAGAGCAGAGTCATTCTTAACCATAGAGACAGAAGAGATAA GAATCATAACAACAACAACACCAATCATTACACACAGGTTGATACTATGGAGAGAAAGCCTCTTGAGGTTGAGCCATCTA CTACGACGACTAACACCGATGTGGTCGATTCTTTCGAGGAAGAGCAGAGAAAAATCGTTTATAGAGGATGGAAAGTCATG CCTTTTATCATTGGTAATGAGACATTTGAGAAGCTTGGGATCATAGGGACATTATCAAACCTTCTTGTGTACCTAACTTC TGTATTCAACCTTAAGAGCTACACAGCTGCAACTATCATCAATGCCTTTAGTGGTACAATCAATTTCGGGACTTTTATTG CTGCCTTTCTTTGCGACACTTACTTTGGTCGCTACAAGACTCTCAGTGTCGCTGTCATCGCTTGTTTTCTGGGATCGTTT GTGATACTACTTACTGCTGCAATTCCGTCGTTGCACCCCGTTGCTTGCGGAAACAAAATCTCGTGCGAAGGGCCAAGTGT GGGACAGATACTGTTTCTGCTCATGGGTTTAGGCTTTCTTGTGGTAGGCGCTGGTGGTATCAGACCGTGTAATTTAGCGT TTGGTGCTGATCAGTTCAACCCCAAATCCGAATCAGGAAAGAAAGGGATCAACAGTTTCTTCAACTGGTATTTCTTCACT TTCACGTTCGCGCAGATCATCTCGCTCACAGCAGTTGTGTATATCCAGTCAAATGTGAGTTGGACAATCGGTTTGATCAT TCCGGTGGCTCTTATGTTCTTGGCGTGTGTGATTTTCTTTGCTGGCGATAGATTGTATGTAAAAGTGAAAGCCTCGGGTA GTCCATTGGCTGGTATTGCTCGTGTTATAGCAGCCGCGATCAAGAAACGAGGTTTGAAGCCGGTTAAGCAGCCTTGGGTC AATCTTTACAACCATATCCCGTCTAACTATGCAAATACTACTCTAAAATACACCGACCAGTTCAGATTTCTAGACAAAGC AGCAATTATGACCCCCGAGGAGAAGTTGAACTCCGATGGAACGGCTTCCGATCCATGGAAACTATGCACGTTGCAGCAAG TGGAAGAAGTGAAATGCATTGTAAGAGTGATTCCAATCTGGTTTGCATCCACGATATACTACCTTGCAATAACTATACAA ATGACTTATCCGGTCTTCCAAGCGCTCCAGAGCGACCGACGATTAGGTTCTGGTGGCTTCAGGATTCCTGCGGCCACCTA TGTAGTGTTCTTGATGACTGGCATGACAGTTTTCATCATATTCTACGACCGTGTACTTGTCCCGTCGCTCAGAAGAGTGA CCGGGTTAGAAACCGGTATATCACTCTTACAAAGAATCGGAGCTGGGTTTACATTTGCGATAATGAGTTTATTAGTTTCC GGGTTTATAGAGGAAAGGAGAAGAAACTTTGCATTGACCAAACCGACACTCGGGATGGCGCCTCGGACAGGAGAAATCTC ATCAATGTCAGCGTTGTGGCTGATTCCGCAGCTTACACTTGCAGGCATAGCAGAAGCTTTTGCTGCCATTGGACAAATGG AGTTTTACTACAAGCAGTTTCCTGAAAACATGAAGAGCTTCGCGGGTTCTATCTTTTATGTCGGTGCAGGGGTTTCGAGC TACCTCGCTAGCTTCTTGATATCAACTGTTCATCGAACAACCGCGCATTCACCTTCAGGGAATTGGTTAGCTGAGGATCT GAACAAAGCGAAATTGGATTACTTCTACTTCATGCTCACAGGACTCATGGTTGTTAACATGGCTTACTTCTTGTTGATGG CTAGATGGTATAGATACAAAGGAGGTAATGATGAAGACATCACTGAGATTGAGACCAATGAAGAAGAAACCAAGCAACAG CAGCTACAAGACAAGAACTCTGTCTGATCTGGAAACTTATATATTTTTGGTATTCTACTTCAATCTCCGTAGTTTTTCTT CACCCGAAATCAACCTTGTTGTACTTTTATAGATTAATTGAAGAAG ................................................................................ ....................................i........................................... ..............................................i................................. ..................N................................................N.........i.. ................N............................................................... .........................................N...................................... ................................................................................ ................................................................................ ......................i......................................................... ................................................................................ ....................................................N........................... .............N..........................................N....................... ................................................................................ ............................N................................................... .......i...........................N..................i........i................ .............i.................................................................. i..............................................................................N .............N........N......................................................... ..............................................................N................. ......................................................i......................... ....N.......................................................................i... .............................i...........................i...................... ................................................................................ ...............................N..............i...........i.................N... ....N......................N..N..........................N...................... ................................................................................ .............................................. Pos Score Pred ------------------------ 117 0.732 Yes 207 0.906 Yes 259 0.116 - 308 0.468 - 318 0.559 Yes 337 0.207 - 442 0.255 - 663 0.577 Yes 853 0.384 - 894 0.417 - 937 0.080 - 1069 0.200 - 1128 0.730 Yes 1156 0.089 - 1175 0.544 Yes 1184 0.693 Yes 1214 0.692 Yes 1281 0.692 Yes 1360 0.114 - 1374 0.322 - 1383 0.191 - 1503 0.362 - 1575 0.654 Yes 1605 0.421 - 1677 0.501 Yes 1710 0.586 Yes 1738 0.524 Yes 1872 0.378 - 1887 0.804 Yes 1899 0.913 Yes 1917 0.489 - 1925 0.403 - 1948 0.284 - 1951 0.318 - 1978 0.149 -

Explain the output -- Processing locus 149: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTACATTTTTTTTTATTTTTTGTTGGTTTGGCAATTTTTCCGGTAAACTATGCAAATTCTATTCGCCGAAACATTTTG CAGATCACTAAACCAAACTGAATCACACGCGCGAGCCAAATGCGCGTGAGATTTCATCACCTTCGTCAAAATACTATCAC ATCACACCAAACCCCTGAGCCGACTATAAAACCTTTTAGATCTCTCCCTCGTGTTATAACTCCGCCACCAACAATATTTT TCTCTACTCAATTCACTCACACTCCAAAAGCGGTAGCAATGTCAGCCACCGCCGCCGCCTCCTCCTCGATCGCCGTTGCC ACCAACTCTCTCAGGAACGTGACTCTGTCTTCCAGATCGCCCCTTCCATCAGCCATCTCCGTGGCTTTCCCTTCCCGTGG ACGCAATACTCTTCAGCGACGCCTCGTTCTCGTTTCCTGCAGTACTGGAGACGGATCTAAGCCTACGATTCTCGTGGCGG AGAAGCTCGGTGACGCTGGTATTAAGCTTTTGGAGGATGTCGCGAATGTCGACTGTTCGTATAACATGACTCCTGAGGAG CTCAACATTAAGATCTCGCTCTGTGACGCCTTGATCGTGAGGAGTGGGACTAAGGTTGGCCGTGAGGTGTTCGAATCGTC TCACGGACGGCTCAAGGTTGTTGGTCGCGCTGGTGTTGGGATCGACAACGTGGATCTGAGTGCAGCGACGGAGTTTGGGT GTTTGGTTGTCAACGCTCCGACGGCGAACACGATCGCTGCCGCTGAGCACGGAATCGCTCTTATGGCCGCCATGGCTAGG AACGTAGCTCAAGCTGATGCGTCTGTTAAGGCTGGAGAGTGGAAGAGGAACAAGTATGTAGGTGTTTCACTTGTGGGAAA GACCCTTGCAGTGTTGGGATTCGGGAAAGTTGGGACGGAAGTTGCTCGTCGTGCTAAGGGTCTTGGTATGCGTGTCATTG CTCATGATCCCTATGCCCCAGCAGACCGTGCACACGCCATTGGTGTTGACTTAGTGAGCTTTGATGAAGCCCTTGCCACC GCGGATTTCATTTCTCTTCATATGCCTCTTACGCCAACAACATCGAAGATACTAAACGACGAAACCTTTGCCAAAATGAA GAAGGGAGTTCGAATTGTTAATGTTGCTCGTGGAGGTGTCATAGACGAAGATGCGTTAGTGAGAGCTCTGGATGCTGGTA TTGTTGCGCAGGCTGCACTTGATGTTTTCACCAAAGAGCCTCCGGCTAAAGATAGCAAGCTCGTGCAGCACGAGAGGGTC ACTGTGACACCTCATCTTGGAGCTAGTACCATGGAGGCTCAGGAAGGAGTTGCCATCGAAATTGCTGAAGCTGTTGTTGG AGCTCTGAATGGAGAGCTTGCTGCTACTGCAGTCAATGCACCTATGGTTTCTGCTGAGGTTCTGACCGAGCTGAAACCAT ATGTGGTGCTAGCCGAGAAACTAGGAAGACTGGCAGTGCAACTGGTGGCCGGAGGAAGCGGTGTGAAAAACGCGAAAATC ACATACGCCTCAGCAAGAGCCACTGATGATCTTGACACCAGACTTCTCCGAGCCATGATCACCAAGGGAATCATCGAGCC AATCTCTGACGTATATGTCAATTTGGTCAACGCTGATTTCACAGCCAAGCAGAGAGGTCTCAGACTCTCAGAGGAACGTG TGCTTTTGGATGGTTCACCAGAGAGCCCATTGGAGACAATAACTGTTCAGCTAAGCAATGTGGAGTCCAAATTCGCCAGT TCCCTGTCTGAATCAGGAGAGGTTAAAGTGGAAGGAAAGGTTAAAGACGGAGTCCCGCATTTGACAAAGGTTGGATCTTT TGAGGTCGATGTAACTCTTGAAGGTAGTATCATACTGTGCAGGCAGGTGGATCAACCTGGTATGATCGGGACTGTCGGAA GCATCCTTGGAGAGTCTAATGTCAATGTTAACTTCATGAGCGTTGGGAGAATTGCACCGAGGAAGCAAGCGATTATGGCG ATTGGAGTAGATGACATACCAAGCAAAGAGACACTGAAGAAGATTGGGGAAATTCCAGCGGTTGAGGAGTTCGTTTTCCT CAAGCTCTAGTTTGGTGTGTTGTTGATTCTTGAATCTTAAATCTCACCGGTGAAGATTTGATGTTGTTTGTTGGTTTAGG CAATAATGTTATTTTGGCTTTCATACAGT ...................................................N............................ .......................................i........................................ ................................................................................ ......................................i......................................... ................................................................................ ................................................................................ ....................................N........N...................i.............. ................................................................................ ................................................................................ ..............................................................i........i........ ................N......................................N........................ ...................................................................i............ ...N........N..................................................N................ .....................i.....................................................i.... ....................N.............................N....................N........ .....................N.......................................................... ..............................i................................................. ........N..........................N.......i.................................... N............................................................................... .........................N............................i......................... ..............N................................................................. .........N...............................................N...................... ................................................................................ ........N....................................................N.................. ..................N.....N..........N......................................i..... ..........N..................................................................... ............................................................N................... .....N....................... Pos Score Pred ------------------------ 52 0.391 - 120 0.587 Yes 279 0.898 Yes 517 0.140 - 526 0.356 - 546 0.654 Yes 783 0.697 Yes 792 0.731 Yes 817 0.150 - 856 0.145 - 948 0.550 Yes 964 0.100 - 973 0.123 - 1024 0.113 - 1062 0.657 Yes 1116 0.868 Yes 1141 0.134 - 1171 0.147 - 1192 0.136 - 1222 0.080 - 1311 0.861 Yes 1369 0.151 - 1396 0.120 - 1404 0.760 Yes 1441 0.428 - 1546 0.204 - 1575 0.639 Yes 1615 0.351 - 1690 0.217 - 1738 0.331 - 1849 0.119 - 1902 0.402 - 1939 0.152 - 1945 0.117 - 1956 0.450 - 1995 0.596 Yes 2011 0.104 - 2141 0.068 - 2166 0.047 -

Explain the output -- Processing locus 150: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGGAATGAGTCCTACACGTAGTCACATCACATATGAAAGAGATTGCTTTTGAAAATCGAATCCTCTTCTAATATTAAATC TTTCAAGACTGGAGGTTTTAAACAAGAAAATCAAACTCGATGCAAAAGAACAAAATGGTCGACTTGCGAGCAATCTTCTG GTTTGTGGTCATAAGCTCTTGTGCTGTCGCTGCACCAACCTGCATTCAGAGATCTGATTTTTTCAAAGCCAATGGTCCTT ACGACATAAACCTCCGAGCCATGCTCTCGTCTCTTCCTTCTCGTGTCAAGGATAATGAAGGCTTCTACAAGACTCCGTTC AAGCCCGGTCCAAACATAGCACATGGTCTGGGGATGTGTAGCCGAGGTACTACAACACAGGATTGTTCGGATTGTATCAC GAGTGTATCTCATACCTTGCTACACACATGTCCTAATCAGGCAGAAGCTATAGACTGGAGTTCTGGAGACTCGCTTTGTT TGGCTTTGATGGATCGTGTGATAAACAAAGTTGATGGTTCATTGTACGCCAACAGCATACAAGAGCTCGGGTCGTTCCCG TTCAGGAGCATTTATGCCATCGCGCAATGCAATAAAGACCTAACCAAGTTGAACTGCGAGAAATGTTTACAACATCTTAG GATAGATAATAGGTCATGCTGCCGGGGGATACAAGTAGGCTATATTGCAAGGACCAGCTGTTTCATGCGATGGGATCTAC AGCCATTCTTGGGACTATTCATAAATGGGATGTTACCAACTCCCCCTTCTGAATTAGATAATGGTCACTCTAACACAACC AAGAAAGATGGCAAAAATATATCTACAGGATCTATTGTGGCAATTGCGGTTGTTTCCGTCGTCGTGAGCACTGTACTGCT TGCTCTTGGATACGCTGTTTCTAGGAGGAGAAAAGCTTACCAATCATTTGCAAGTGAAAATGGGTATTTCTCTGTTTCAA GAAGACCTAGGAGGCCTTATGGTACTGCATCACCAGATGATGCTACGGATGATCTTACAGCGTCCTCAGGTTCGCTTCGA TTCGATTTTAGAGCAATAAAAGCTGCAACAAGTAATTTTCATAAGAGTAACAAGCTTGGTCATGGTGGATTTGGTGCAGT TTACAAGGGTATGTTTCCGAATGGAACAGAAGTTGCTGCCAAGAGACTATCTAAACCATCAGATCAAGGTGAGCCAGAGT TCAAGAACGAGGTGCTTCTTGTAGCAAGACTTCAGCATAAAAATCTTGTTGGACTTCTCGGGTTTTCGGTTGAAGGAGAA GAGAAGATACTAGTTTACGAGTTTGTTCCCAACAAAAGTCTCGATCATTTCCTTTTTGACCCGATAAAAAGGGTTCAACT GGATTGGCCAAGACGACACAACATTATCGAGGGAATCACTCGAGGAATTCTCTATCTTCATCAAGATTCACGGCTCACAA TCATACACCGTGACCTCAAAGCCAGCAACATTCTCTTAGATGCCGAGATGAATCCAAAAATAGCTGATTTTGGTCTGGCT AGGAATTTCAGAGTTAATCAAACCGAAGCAAATACAGGAAGAGTAGTTGGCACATTCGGTTACATGCCACCTGAGTATGT GGCAAATGGACAGTTCTCAACGAAATCTGATGTGTATAGCTTTGGAGTATTAATTCTGGAGATTATTGGTGGCAAGAAGA ATAGTAGCTTCCATCAGATAGATGGTTCAGTAAGCAACTTGGTCACACATGTTTGGAGGCTTAGGAATAATGGATCGTTG TTGGAACTTGTAGATCCAGCCATTGGGGAGAATTATGATAAAGATGAAGTCATTAGATGCATTCACATTGGGTTATTATG CGTTCAAGAAAATCCTGACGATCGTCCAAGCATGTCCACAATATTTCGAATGCTAACTAATGTTTCAATTACTCTGCCTG TGCCTCAACCACCTGGATTTTTCTTCAGGGAAAGATCCGAGCCGAACCCATTAGCCGAGAGATTACTGCCTGGTCCGTCT ACTAGCATGTCTTTTACTTGTTCGGTAGATGATGCATCGATCACGAGTGTTAGACCTCGTTAAAATTGGGCTAATGATTA ACATGAATATTTTTCATAAGTCTAAAAATATGTTATTCTCTCTCTTTCAAAATACATTGAATTTGGTATTAGTTACTTTT AT ....i...........................N............................................... .......................................N..............i......................... .......................................................................N........ ....................N.................................i......................... ......................N..........N.............................................. ...........................N.................................................... ........i........................N.............................................. .............N............N...................................N................. ...............i................................................N....N.......... ........................N....N..............................N................... .......N........................................................................ ...........................................................i.................... ..................N.................N..N........N............................... .............................................................N.................. ..........i.........N........................................................... ................................................................................ ................................................................................ ................................................................................ .......................................N.......i................................ ...............................................................N............N... .....N.......................N.................................................. .....................N..........................N....................N.......... ..................................N........N............i....................N.. ...............................N.................N.........N.................... ................................................................................ ......N.....................N..N.........................................N...... ..N..........................N.................................................. .. Pos Score Pred ------------------------ 5 0.524 Yes 33 0.341 - 120 0.246 - 135 0.566 Yes 232 0.366 - 261 0.410 - 295 0.724 Yes 343 0.385 - 354 0.456 - 428 0.480 - 489 0.612 Yes 514 0.237 - 574 0.326 - 587 0.483 - 623 0.384 - 656 0.591 Yes 705 0.225 - 710 0.283 - 745 0.482 - 750 0.312 - 781 0.182 - 808 0.332 - 940 0.557 Yes 979 0.208 - 997 0.250 - 1000 0.162 - 1009 0.264 - 1102 0.395 - 1131 0.608 Yes 1141 0.141 - 1480 0.372 - 1488 0.822 Yes 1584 0.441 - 1597 0.255 - 1606 0.216 - 1630 0.200 - 1702 0.236 - 1729 0.173 - 1750 0.236 - 1795 0.303 - 1804 0.083 - 1817 0.528 Yes 1838 0.448 - 1872 0.432 - 1890 0.302 - 1900 0.473 - 2007 0.452 - 2029 0.113 - 2032 0.154 - 2074 0.101 - 2083 0.145 - 2110 0.074 -

Explain the output -- Processing locus 151: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCTGAAGCTTCCTTTGTGGACTTCTGCTGTTTTTTTCCTAACTTTGGAATAGTTTTTGTTTATCTCTGGACACAGAGA TCGCAAATTCTTTTTTCTTAAATGGAAGGTTCCATGAACAAGCGAGGATACGAATGCAGTCAAGAGGATACTGACAAGCT GCCAGAATCAAAGAGACAAAAAGTGCCTGCTTTAGCAAGTGTTATTGTGGAAGCTGTCAAAGTAGATAGTCTGCAGAGGC TTTGCTCGTCTTTGGAGCCGTTGTTTCGCAGAATTGTTAGCGAAGAAGTGGAACGAGCCTTATCAAGGTTGGGAAACGCA AAGTTAACCTCGAGGTCACCAGAACCAAAGAGGATCCAAGACCGCAATGGAAGAAACCTGCAACTTCACTTTAGGACGCG AATGCCTCCTCACTTATTCACAGGCGGGAAAGTTGAGGGAGAGCGAGGCTCGGCAATTCATGTGGTTCTTATAGATGCAA ACACAGGAAATGTAGTTCAAACAGGAGAAGAATCAGCTTCAAAGCTGAATGTTGTAGTGTTAGAGGGTGACTTCAATGAT GAAGACGATGAAGACTGGACCAGAGAGCACTTTGAGAGCTTTGAAGTGAAAGAGCGAGAAGGGAAACGCCCCATCTTAAC TGGGGACACACAGATAGTGCTTAAGGAAGGTGTGGGAACTCTTGGAGAACTTACTTTCACTGACAACTCGAGTTGGATAC GTAGCCGAAAGTTTAGGCTTGGTGTTAAGCCGGCTTCAGGGTATGGTGATAGCTTTTGCATTCGTGAAGCCAAAACAGAA CCTTTTGCTGTCAAAGATCATAGAGGAGAACTATACAAGAAACATTATCCACCGGCTGTACACGATGAAGTCTGGAGACT GGATCGAATAGCGAAAGACGGAGTGCTACACAAGAAGCTATTGAAAGCTAATATTGTGACAGTCGAAGACTTCCTTCGAC TCCTTGTTAAGGATCCACAGAAGCTGAGAAATTTGCTTGGGAGTGGAATGTCGAATAGAATGTGGGAGAACACGGTGGAG CACGCAAAGACGTGCGTGTTGGGTGGGAAGCTTTATGTGTTTTACACAGACCAAACTCACGCTACAGGCGTTGTCTTCAA TCATATATATGAATTCCGAGGCCTAATTACAAACGGACAGTTCCTATCTCTAGAGTCTCTTAACCATGACCAAAAGATTT CTGCAGACATTTTGGTGAAGCTGGCTTACGAGAACTGGCATAAAGCTATTGAGTATGACGGTAAGCTGTTGAATTGCTTA CCAGTTGCCGAGAAGGAAATAAAAAGCTTACTGGAACCAAAAATGGTCTCAGCACAAACTGCTCCGAACCATCAACAGCT GCATAACCAGAACAATAGGCAAACTGTGCAAGGTCATCAGAATGCGATTACCTATTCACCGGTCCCTCAACCAATAGATT ATCCTCAGTTTGCGCAGCAACATTGCAACCAATTATTACCCTCATTCCCTTGCAACGTACAAGACTACAACAGGTCGATG GAAAGCTCCAATGATTCAAGCTCATACAATGGAGAAGATTGGTGTCCACCAAGAGCTGCAGGACAAGGTCTTGAAGACAT TTTCAGCGAAGAAATCAGACTAAGGAGTTCTGAAATGCTTGAGACTGACGATATGCAGAGACTGTTGAAGACGTTTGGTA TCGGTGTGAACACTGTGGGAACACAAGGAGGGTTTGGTCAGACTGATGAGTCTTGTTACGGTTATAGCATCCCGTACCAA GCTCAGATCGATAACACGTACCGAAGAGAACGTAATAGAGGCTCGGGGAAAGCTGTTGTTGGATGGCTAAAGCTTAAAGC TGCTTTGAGATGGGGTATCTTTATACGCAAGAAAGCTGCAGAGAGAAGGCCTCAGATTGTGGAGATCGATTGACAAAAAG GGATAGAGAGCTTCTTGAGGTTTTAATCCGTTGAAGATTTTTCGTTTTCGTTAAAGCTTGAAGACAGTTTCTGTGTGTTC ATCTTTTGGATG ................................................................................ .....................i...........N...................N.......................... ................................................................................ ................................................................................ ..............................................N................................. .........N......................................N..........................N..N. .......N........................................................................ ................................................................................ ..........................................N..................................... ................................................................N............... ................................................................................ ...............................................N...........i.................... ..................................N............................................. ........N........................................................N.............. ......................................................N......................... ..........................................i..................................... .........................................N...................................... .............................................................................i.. ..........i.................i................................................... ..................................i.................i........................... .............................................N.................................. ..............................................................i................. .........i...................................................................... ................................................................................ .........N.. Pos Score Pred ------------------------ 102 0.600 Yes 114 0.386 - 134 0.490 - 367 0.482 - 402 0.491 - 460 0.120 - 475 0.363 - 490 0.454 - 529 0.443 - 556 0.130 - 559 0.359 - 568 0.373 - 763 0.461 - 865 0.328 - 1008 0.477 - 1020 0.520 Yes 1075 0.193 - 1129 0.181 - 1186 0.121 - 1255 0.467 - 1323 0.796 Yes 1402 0.369 - 1518 0.738 Yes 1531 0.708 Yes 1549 0.784 Yes 1635 0.758 Yes 1653 0.677 Yes 1726 0.380 - 1823 0.624 Yes 1850 0.585 Yes 2010 0.036 -

Explain the output -- Processing locus 152: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATAACAAAAGCTCACCATCAAACGAAGAATAATAACACAAACAATTAGAACTAGAACAAAAGAAAATGATAAAGCAAT TTTATTCTCATGTAAAAATGACATAATTCAATGACGCGTGTCCCACCAATTATCACCACTCACTCTCCATATATACTTCA CATACAATCTTTCACTCATATATATACCACACAGTCACACATTCTTTATAATGTAAATCAAAAGAGAACTAAACCAAGAA ACAAAATGGGATACGAAAGGCTCGGACCATCCGGAGCAACTGGTTCGGTCACCACCTCCACTACTACAGCTCCAATCCTT AACCAAGTCTCCACGTCAGAGCAACCGGAGAACAACAACCGAAGAAGCAAAAAGAAGCTTGTCGTATCATCAATCGTCTT AGCCATCTCACTTATCCTCGCCGCCGCGATCTTCGCCGGAGTCAGATCACGTCTCAAGTTAAACCAATCAGTCCCAGGCC TAGCTCGTAAACCGAGTCAAGCAATCTCTAAAGCTTGTGAGTTAACTCGTTTCCCCGAGTTATGCGTTGACTCACTCATG GACTTTCCCGGCTCACTCGCCGCTTCCTCCTCTAAAGATCTCATCCACGTGACTGTGAACATGACGCTCCACCACTTCAG CCACGCTCTCTACTCCTCTGCTTCCCTCTCCTTCGTCGACATGCCGCCACGTGCTCGCTCAGCTTACGACTCTTGCGTCG AGCTACTTGACGATTCCGTTGACGCGCTATCACGCGCTCTTTCCTCCGTCGTATCCTCCTCCGCTAAGCCGCAAGATGTT ACAACGTGGCTTAGCGCGGCTTTAACAAACCATGACACGTGTACTGAAGGATTCGACGGCGTCGACGACGGTGGAGTGAA GGATCATATGACGGCGGCGTTACAGAATCTTTCTGAGCTTGTTAGTAACTGTCTTGCTATATTCTCGGCGAGTCACGACG GTGACGATTTCGCCGGAGTTCCAATACAGAACAGGAGGCTGCTGGGAGTGGAGGAACGAGAAGAGAAATTCCCGAGATGG ATGAGACCAAAAGAACGCGAGATACTGGAAATGCCGGTATCTCAGATACAAGCTGATATTATCGTATCGAAAGACGGTAA CGGCACGTGCAAAACTATATCGGAAGCTATCAAGAAAGCTCCTCAGAACAGTACTCGCCGGATAATTATCTACGTCAAGG CCGGAAGATATGAAGAGAACAACCTCAAGGTCGGTAGGAAAAAAATTAATTTGATGTTCGTTGGAGATGGCAAGGGTAAA ACTGTTATTTCGGGAGGCAAAAGTATATTTGACAACATCACTACTTTCCACACGGCGTCATTTGCTGCGACCGGAGCTGG ATTTATTGCAAGGGACATCACATTCGAAAACTGGGCCGGTCCAGCGAAGCACCAAGCCGTGGCTCTCCGCATCGGAGCTG ACCACGCAGTGATCTACCGATGCAATATAATCGGTTACCAAGACACACTCTACGTCCATTCAAACCGCCAATTCTTTCGT GAATGCGATATCTACGGTACAGTCGATTTTATCTTTGGTAACGCAGCCGTCGTACTACAAAACTGTAGCATCTACGCACG GAAGCCAATGGATTTTCAGAAAAATACAATCACGGCTCAAAACAGAAAAGATCCGAACCAAAACACCGGAATATCAATAC ATGCGTCTAGGGTTCTTGCAGCATCCGATCTCCAAGCGACAAACGGTAGTACACAGACGTATCTAGGCCGACCTTGGAAG CTATTCTCTAGGACAGTGTATATGATGTCGTATATCGGTGGTCATGTACACACGAGAGGTTGGCTCGAGTGGAACACAAC ATTTGCTTTAGATACTTTGTATTATGGAGAGTATTTGAACAGTGGACCTGGGTCGGGTCTCGGGCAACGTGTGAGCTGGC CGGGTTATCGGGTCATCAATTCGACGGCCGAGGCAAACCGGTTCACTGTGGCAGAATTTATATACGGTTCCTCGTGGTTG CCATCGACCGGAGTTTCGTTCTTGGCCGGATTAAGCATATAGGTAGAGAAAAAATTGAAATGCTTATTTTTCCGATGAAT TGTTTCATTCTTACGGTCCTTATATTAATGGACATATCAGTATTCCAATATATATGAGTAT ...................................................................i............ .........N.......i............i................................................. ..................................................N............................. .....i.......................................................................... ................................................................................ ................................................................................ .............................................................i...............N.. ............................................................i................... ........................................i....................................... ...........................................................................i.... ...............................N................................................ .......i........................................................................ ............................................................................i... N.............................i................................................. ................................................................................ .........N...........................................i............N............. ................................................................................ ................................................................................ ...................N............................................................ ..N............................................................................. .......i........................................................................ N............................................................................... .....................i..i..................N.................................... .......................N........................................................ ................................................................................ ...........................................................N..............N..... ...........................N.........................N....... Pos Score Pred ------------------------ 68 0.643 Yes 90 0.150 - 98 0.513 Yes 111 0.521 Yes 211 0.483 - 246 0.872 Yes 542 0.586 Yes 558 0.430 - 621 0.829 Yes 681 0.807 Yes 796 0.622 Yes 832 0.482 - 888 0.805 Yes 1037 0.555 Yes 1041 0.478 - 1071 0.582 Yes 1210 0.181 - 1254 0.614 Yes 1267 0.137 - 1460 0.240 - 1523 0.344 - 1608 0.736 Yes 1681 0.277 - 1782 0.560 Yes 1785 0.509 Yes 1804 0.358 - 1864 0.404 - 2060 0.096 - 2075 0.081 - 2108 0.032 - 2134 0.042 -

Explain the output -- Processing locus 153: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTTTTGTGAGGACTCACAATCTTTCTCATTTAATACATGAGCAGACACGGCACCTAACTAATGGCCCAAAGGTGGCCCG CCAAATTGAATACAAGGGTAAGAAATGGGCCAAGAAAGTGAGAGCCCAAAAGAGGCCGATCGTTCGTTTTTGACTCAATG GCCGTTGACGTTGACATCGTACGCCGCACCCAACGACCGCCAAACAGCAAACAACAACACTTTCCCCTTTCTCTCTCTAA ACATGGCGATGCTTTCTACTGCTTCCGTTTCGGGGAGCGTCGACCTTCCTCGAGGAACCATGAAGGTTGACTCGTCTGCG TCTCCGGAGGTCGTATCCGATCTCCCACCATCTTCCCCAAAGGGCTCACCTGATCGCCATGACCCTTCCACTTCTTCTCC GAGCCCCAGTCGTGGTGGTGACAATCAGTCTGAAGTCATATCCAAGAGTGAAGAGTATCGGCAATTGTTTCGGCTTCCAG CTGACGAAATCCTTGTTCAAGATTTTAACTGTGCTTGTCAGGAGAGTATTCTTATGCAGGGTCATATGTACCTCTTCATC CATTATATCTGCTTTTACTCCAACATCTTTGGTTATGAGACCAAGAAAATTATCCCATTTGCTGAAATATCTTGTGTTAA ACGAGCCAAGACTGCTGGTATTTTTCCTAATGCCATTGAGATTTTAGCTGGAGGGAAGAAGTACTTTTTTGCATCGTTTC TTTCCCGCGATGAAGCTTTCAAGCTTATCCATGATGGATGGTTGGAATATGGTAGTGCTGTCAAATCAGAAGGCGAGATT CTGGTGACCGAACCGCAGGTAAGCGATGGAGTTGTTAAAAGGGCACGCAGTTCCATGGATTTGGCCAATGAACTAGATAT TCCAGTAAGGGATGAAACTCTTCATCTTTCCAGCAGTTCCAGTCTTCCTGTCATTAGTCAGAATGGCGTTCCACCTTCAT CCGTTCAGCGGCACGCTGAACCAGATGTAGATGTTGTGGCGGCCAATACTTTCAACTGGAAGCCTGAAGATACAGATGCA CCTAAATTGTCATCTGACTTTACAAAGGTAGCAGAGGCAAAGTTTTCGATTCCAGTAGAAGAGTTCTTTAGATTGTTTTT TTCAGATGGTGCTGTCAGTTTTGTTGAATCTTTCCATAAAAATTGTGGAGACAAAGAGTTTAGGTGTACCTCTTGGCAAC CTCATGAAAAGCTTGGACACACCCGCAATGTCTCATTTCAACATCCGATAAAAATTTATTTTGGTGCAAAGTTTGGTGGC TGCCAGGAGTCACAGAAATTTCGGATGTACAGAAATAGCCATCTGGTTATTGAAACATCACAGGAGATCAGCGATGTGCC TTATGCAGATTATTTTACGGTAGAGGGAGTCTGGGACTTGAAAAGAGATTGCAGAGACTCGGTAGAAGGTTGTATATTGG ATGTTTATGTCAATGTGGCCTTCTCTAAAAGAACAGTGTGGAAAGGGAAAATAGTGCAGTCTACTTTAGAAGAATGTAGA GAAGCTTATGCACATTGGATAAGAATGGCACATGAACTGTTGAAGCAGAAGAAGCTGGAGAACCAAGAAGGGAATAAGTT GATTGAGGATGGTGAACCGCTAGCAGCAAGGGAAGAAAGAGTATCTGAATGTGACGAGGAGGGGAAGGTGGAAATGGTGG GCGAAGGGGTAGTTAAAAAAAGCTTAAAGGAAGCATGGGTGAATCTGACTTCATTTGTGAAGAGGCAAAGCGGGACAAGG CAGGTTATAGTATTAGCGTTTGCAGTAATCTTACTGATGCAGGTGACGATAGTGGTGCTACTGAAAAAAGGAGGAGGGGG GCAAGTGGAGTATCATGAGAGGTACGACGAGTACAGTGTAAACGGGGAGACTTTGGGGTGGTTAGAGAAGAGAATGCATT TCCTGAGGGAAGAGATGATGATGGTTGAGGATCGTTTGCAGAGGATGCGACAAGACCATGCCGCCTTGAAGGCTCAGTTC CACCATTTGGAACGCCTCCTCCGCCGCAACAAACAGTAAATTTGCTACACCCTACTCTCTGTTTGTGCAGAATGCGGATG GATATATGCATGTATGTATGTAATGTATTATCGGACGTTACTTTGCAAAGTTGTTGTT .....................................N.......................N.................. ........................i....................................................i.. ................................................................................ ..i.....i..................................................i.................... ..........................................................N..................... ................................................................................ .....................................................N...........N.............. ..................................N............................................. .............................N.................................................. .........N....................N..N...N..........N............................... .........................N............................i............N............ ...........N..................................................N................. ........................N.....N............................................N.... ................................................................................ .....i.......................................................................... ...N.......................N.................................................... ........................i................................................N...... ..N............................................................................. N.....N.....N............................................................i...... .......N................i......N................................................ ........N.......................................N........................i...... ..................................i............................................. ....................................N........................................... ..............N..........................................................N...... ..............N..i..N.......................N............N...................... .......................................................................N.....N.. .....N...N...N...N....N................................... Pos Score Pred ------------------------ 38 0.393 - 62 0.456 - 105 0.768 Yes 158 0.707 Yes 243 0.846 Yes 249 0.849 Yes 300 0.789 Yes 379 0.482 - 534 0.412 - 546 0.322 - 595 0.207 - 670 0.295 - 730 0.268 - 751 0.385 - 754 0.399 - 758 0.406 - 769 0.215 - 826 0.274 - 855 0.609 Yes 868 0.156 - 892 0.201 - 943 0.373 - 985 0.110 - 991 0.142 - 1036 0.106 - 1126 0.752 Yes 1204 0.144 - 1228 0.223 - 1305 0.684 Yes 1354 0.437 - 1363 0.138 - 1441 0.169 - 1447 0.236 - 1453 0.234 - 1514 0.598 Yes 1528 0.182 - 1545 0.755 Yes 1552 0.174 - 1609 0.201 - 1649 0.400 - 1674 0.545 Yes 1715 0.846 Yes 1797 0.440 - 1855 0.222 - 1914 0.252 - 1935 0.361 - 1938 0.685 Yes 1941 0.243 - 1965 0.093 - 1978 0.423 - 2072 0.101 - 2078 0.072 - 2086 0.036 - 2090 0.086 - 2094 0.058 - 2098 0.048 - 2103 0.095 -

Explain the output -- Processing locus 154: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAGAAATAAAGGTACTTAATTCTTATTCTTTACGCGCTACAAAATAAGCAACAATGGCAAAACCGTAAAAATGAGTAGT CAAAGGGGTATTTGGTTTTAGAAAGAGATGAGGATAAAAATGAGGTTAAGCGTTTTTATTATTCCCCTGGTGGAAAGTAG GCAAGCAAGTGGTATCATCAACAAACAAAGCACTTCTCTTTTGATTCGATTCTCTCTTTACCTTGAAGCTTCAATATCAA CCAAATCTTTTTTCTCAAAATCTCAAAGAATCTCTCAAACTCAGTCTCCTATCTGTCTCTCTGCAAACTATTACCAACCA GATAATTTAGATATGGAAGCTACTCAGAAACATATGATTCAAGAAGGATCTTCAATGTTTTACCATCAACCATCTTCAGT CAAACAAATGGACCTTTCTGTTCAGACATTTGATTCTTACTGCACACTTGAATCCTCTTCAGGCACCAAGTCTCATCCTT GTCTTAACAACAAAAACAACTCTTCTTCAACCACAAGCTTTTCCTCTAATGAAAGTCCCATTTCTCAAGCCAACAACAAC AACTTATCAAGGTTTAACAATCATTCCCCTGAAGAAAACAATAACTCTCCCTTGAGCGGTTCTTCAGCGACAAACACCAA CGAGACAGAGCTTAGTCTCATGCTTAAAGATTTGGAGACTGCAATGATGGAGCCTGATGTAGACAACAGCTATAACAATC AAGGCGGGTTTGGACAGCAGCATGGAGTTGTTTCTTCAGCTATGTATAGATCTATGGAGATGATCTCTAGGGGAGATTTA AAAGGAGTGCTCTATGAATGCGCAAAAGCTGTTGAAAACTATGATTTGGAAATGACTGATTGGTTGATCTCTCAGTTACA GCAAATGGTTTCTGTTTCTGGTGAGCCTGTTCAGCGCTTAGGAGCTTATATGCTTGAAGGTCTCGTTGCTAGGTTAGCTT CTTCGGGTAGCTCCATCTATAAAGCATTGAGATGCAAAGACCCAACGGGTCCTGAGCTTCTTACTTATATGCATATCTTG TATGAAGCCTGCCCTTATTTCAAATTCGGTTATGAATCTGCTAATGGAGCTATAGCTGAAGCTGTGAAGAACGAAAGTTT TGTGCACATTATCGATTTCCAGATTTCTCAAGGTGGTCAATGGGTGAGTTTGATCCGTGCTCTTGGTGCTAGACCTGGTG GACCTCCGAACGTTAGGATAACGGGAATTGATGATCCGAGATCATCGTTTGCTCGTCAAGGAGGACTTGAGTTAGTTGGA CAAAGACTTGGGAAGCTAGCTGAAATGTGCGGTGTTCCGTTTGAGTTCCATGGAGCTGCTTTATGCTGCACGGAAGTCGA AATCGAGAAGCTAGGAGTTAGAAATGGAGAAGCGCTCGCGGTTAACTTCCCGCTTGTTCTTCACCACATGCCTGATGAGA GTGTAACTGTGGAGAATCACAGAGATAGATTGTTGAGATTGGTCAAACACTTGTCACCAAACGTTGTGACTCTGGTTGAG CAAGAAGCGAATACAAACACTGCGCCGTTTCTTCCCCGGTTTGTCGAGACAATGAACCATTACTTGGCAGTTTTCGAATC AATAGATGTGAAACTCGCTAGAGATCACAAGGAAAGGATCAATGTTGAGCAGCATTGTTTGGCTAGAGAGGTTGTGAATC TTATAGCTTGTGAAGGTGTTGAAAGAGAAGAGAGGCACGAGCCACTAGGGAAATGGAGGTCTCGGTTTCACATGGCGGGA TTTAAACCGTATCCTTTGAGCTCGTATGTGAACGCAACAATCAAAGGATTGCTTGAGAGTTATTCAGAGAAGTATACACT TGAAGAAAGAGATGGAGCATTGTATTTAGGATGGAAGAATCAACCTCTTATCACTTCTTGTGCTTGGAGGTAACTAATAA AAACCTTGTTCGGTTTCAGAAGAGATTAGAAACTTCTTTTAAAGTTTGCAGAATCTGTTTGTAAAAGTAAAACTCATGCA TGATCCGAAGGA ......................................................N................i........ ...........................N...........i........................................ ................................................................................ ................................................................................ ............i....................N....................N......................... .......i........................................................................ ................................................N............................... ................................................................................ ...................i.......................i..i.........N....................... .....................N...................i...........N.....i.................... .............N...N......................N..........i............................ ....i............................................N.............................. ...............................N....................................N........... .......................................i........................................ ..............................N................................................. ........................i........................N............N................. .......................N...........................................i......N..... ................................................................................ ...................................................N............................ .....N...................................i...................................... ....................................................i..................i........ .........................N...................................................... ...........N..................N................................................. ...........................................................................N...N ............ Pos Score Pred ------------------------ 55 0.442 - 72 0.742 Yes 108 0.390 - 120 0.593 Yes 333 0.665 Yes 354 0.301 - 375 0.412 - 408 0.625 Yes 529 0.375 - 660 0.553 Yes 684 0.507 Yes 687 0.644 Yes 697 0.483 - 742 0.170 - 762 0.604 Yes 774 0.388 - 780 0.656 Yes 814 0.216 - 818 0.471 - 841 0.249 - 852 0.662 Yes 885 0.648 Yes 930 0.302 - 992 0.322 - 1029 0.479 - 1042 0.198 - 1072 0.129 - 1084 0.139 - 1160 0.700 Yes 1231 0.165 - 1305 0.669 Yes 1330 0.139 - 1343 0.324 - 1384 0.493 - 1428 0.523 Yes 1435 0.120 - 1572 0.376 - 1606 0.244 - 1642 0.579 Yes 1733 0.616 Yes 1752 0.510 Yes 1786 0.271 - 1852 0.429 - 1871 0.343 - 1996 0.054 - 2000 0.076 -

Explain the output -- Processing locus 155: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGTTTGAGTAAAGAAAATCCTTTGACACGTCCAATGTAAACTCTTTTAGACGTTTTTTTTGTCCTACTCAAAAAAACTC AACCTCTACTTAAAGTACTAATTACCATGTTTTTGTTTTTTTCTCTACCCTTCACGTGGTTCGCATACAAAGAATATTCC TACACATATTTCTCGTGAAACCTTTAAACATTTTGATTTAGGAGTGTATATATATAAGATGAAAGATAAATGTTTGTCAT TCAGAAACGAGATGACGATAATGACGATGATAATGAAGATGGCTCCTTCCAAATCTGCACTGATACGCTTTAATCTAGTC TTGTTGGGTTTCTCTTTTCTTCTCTACACTGCCATTTTCTTCCACCCTTCTTCATCAGTCTATTTCAGCAGCGGAGCTTC CTTTGTTGGATGCTCTTTTCGTGATTGCACTCCCAAGGTGGTGAGAGGAGTGAAGATGCAAGAACTCGTTGAAGAGAACG AAATAAACAAGAAAGATTTGCTAACCGCTAGTAACCAGACAAAGCTGGAGGCGCCAAGCTTCATGGAAGAGATTTTAACA AGAGGGTTAGGAAAAACAAAGATAGGGATGGTGAACATGGAAGAATGTGATCTTACTAATTGGAAACGTTATGGCGAAAC GGTTCACATACATTTTGAGCGTGTCTCGAAGCTCTTCAAATGGCAAGACTTGTTCCCCGAGTGGATAGATGAAGAGGAAG AAACCGAGGTTCCCACATGTCCTGAGATACCTATGCCCGATTTCGAAAGCTTAGAGAAGTTGGATTTGGTAGTAGTGAAG TTGCCTTGTAATTACCCTGAAGAAGGGTGGAGAAGAGAGGTTTTGAGGTTGCAAGTGAACCTAGTTGCGGCTAACTTGGC AGCCAAGAAAGGGAAGACGGATTGGAGATGGAAAAGCAAAGTGTTGTTTTGGAGCAAATGTCAACCGATGATTGAGATTT TCCGGTGTGATGATTTGGAGAAGAGAGAGGCAGATTGGTGGCTGTATCGCCCTGAGGTGGTTAGGTTACAACAGAGACTC AGTTTGCCAGTCGGATCTTGCAATCTTGCTCTTCCTTTGTGGGCACCACAAGGTGTAGACAAAGTGTATGACCTAACGAA GATAGAAGCAGAGACAAAACGACCAAAACGTGAAGCCTACGTAACTGTTCTTCACTCTTCCGAGTCTTATGTCTGTGGTG CCATAACTTTGGCTCAAAGCCTCCTTCAGACAAACACCAAACGCGATCTTATCCTTCTCCACGATGACTCCATCTCCATT ACCAAACTTCGAGCTCTCGCCGCCGCAGGATGGAAGCTTCGTCGGATCATTCGAATCAGAAACCCACTTGCGGAGAAGGA CTCGTACAATGAATACAACTACAGCAAGTTTCGACTCTGGCAATTGACAGATTACGACAAAGTGATCTTCATTGATGCCG ACATCATCGTCTTACGTAACCTTGATCTTCTCTTCCATTTTCCTCAGATGTCGGCCACCGGAAATGATGTATGGATATAT AATTCAGGCATCATGGTCATCGAGCCTTCTAATTGTACGTTTACTACAATCATGAGCCAGCGAAGCGAGATCGTTTCATA CAACGGTGGAGATCAAGGGTACCTAAACGAGATATTTGTGTGGTGGCACCGATTGCCTCGACGAGTAAACTTTCTGAAGA ACTTCTGGTCGAACACAACCAAAGAAAGAAACATCAAGAACAACCTCTTCGCCGCGGAGCCGCCTCAGGTCTACGCGGTC CACTACTTAGGTTGGAAACCATGGCTTTGCTATAGGGACTACGATTGCAACTACGACGTGGACGAGCAGTTGGTGTACGC TAGTGATGCGGCTCACGTTAGGTGGTGGAAAGTGCACGACTCCATGGACGATGCATTGCAAAAGTTTTGCAGGCTGACGA AAAAGAGGAGAACGGAGATCAACTGGGAGAGGAGGAAAGCAAGGCTTAGAGGTTCCACTGATTATCATTGGAAGATCAAT GTCACTGATCCAAGACGACGTCGTTCTTATTTGATTGGTTAATTTGTTTTTTCCTTGTATTTAGACATCATATCGAGACT GATACCCATTACTCCACCTTTTCTTTATTCTTGTTCTTTGTAGATCATGTCTATGAGTATC ..................................N............................................. ..........................N..................................................... ..........................................................i..........i.......... ...........N........N.....N.....N.....N......................................... ................................................................................ .........N.............................................N........................ ..............................................................N................. ...........................N........N.......N.........................N......... .......................................i............................N........... ................N...............i............................................... ................................................................................ ...........................i.............................i.........N............ .........N...................................................................... ...................................................................N............ ....................................................................N........... ...............................................................N................ .............................i.................................................. ........N.................................................................N..... ...............................................i...............N..N...i......... ............i......................................N............................ ................................................................................ ................................................................................ ....................i........................................................... .....N.....................................N......N............................. ..............................................................................N. ................................................................................ ..............................................N.....N........ Pos Score Pred ------------------------ 35 0.255 - 107 0.134 - 219 0.562 Yes 230 0.536 Yes 252 0.335 - 261 0.429 - 267 0.476 - 273 0.239 - 279 0.400 - 410 0.143 - 456 0.276 - 543 0.207 - 588 0.418 - 597 0.484 - 605 0.388 - 631 0.167 - 680 0.642 Yes 709 0.147 - 737 0.460 - 753 0.537 Yes 908 0.567 Yes 938 0.743 Yes 948 0.294 - 970 0.273 - 1108 0.235 - 1189 0.123 - 1264 0.221 - 1310 0.722 Yes 1369 0.215 - 1435 0.486 - 1488 0.687 Yes 1504 0.441 - 1507 0.444 - 1511 0.792 Yes 1533 0.695 Yes 1572 0.186 - 1781 0.602 Yes 1846 0.304 - 1884 0.322 - 1891 0.282 - 1999 0.130 - 2127 0.072 - 2133 0.046 -

Explain the output -- Processing locus 156: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGATTTGCTTTCAATTTGGGTTTAAATAAGAGTGAGGGTCTCGCAGGTTAGCTTATAAGCCGCCGCCTTGAAAATCCA GAAGGCCATTTTTGTTGGTGAAAGGTAGAATTCGTAAATTTCTTCTGCTCACTTTATTGTTTCGACTCATACCCGATAAT CTCTTCTATGTTTGGTAGAGATATCTTCTCAAAGTCTTATCTTTCCTTACCGTGTTCTGTGTTTTTTGATGATTTAGGTG AAGAAGAAGAAGCAGAGACAAAAACGATTATGAAGTACGTTTTGGTGACAGGAGGAGTGGTGAGTGGTCTTGGCAAAGGT GTCACTGCTAGTAGCATTGGACTTCTCCTTCAAGCATGTGGTCTTCGTGTCACTTCCATTAAAATTGATCCTTATCTTAA CACTGATGCTGGAACAATGTCTCCGTTTGAGCATGGAGAAGTATTTGTATTGGATGATGGTGGAGAGGTGGATTTGGACC TTGGAAACTATGAACGATTTTTAGACAGTACATTGACCCGTGACAACAATATAACTACTGGAAAGATATACCAGTCAGTT ATTGACAAGGAAAGGAAGGGGGACTACCTCGGAAGAACTGTACAGGTTGTTCCTCATGTTACTGACGCAATCCAAGAGTG GATTGAGCGTGTAGCTAATGTTCCTGTGGATGGAAAGGAAGGTCCTCCTGATGTTTGTGTCATCGAATTAGGCGGGACTA TAGGTGATATTGAATCTATGCCCTTTATTGAGGCCCTTGGTCAATTCTCGTATAAAGTTGGACCTGGTAATTTCTGCTTG GTTCATGTCAGCCTTGTGCCTGTTCTCAGTGTTGTTGGTGAACAGAAGACGAAGCCAACCCAGCATAGTGTGCGAGGCCT CAGGAGCCTTGGTTTGACACCAAATATCCTAGCATGTCGCAGCACGAAGGCACTTGAAGAAAATGTCAAGACAAAACTAT CTCAATTTTGCCATGTGCCGGAAGTGAATATAGTAACGCTCTATGATGTTCCAAATATTTGGCACGTTCCTCTGCTTTTA AGGGATCAAAAAGCTCATGAAGCGATCCTAAGAGAGTTAAATCTTAGTAATGCTATAAAGCCTGACTTGACAGAATGGAC TGCAAGGACTAAAATTTATGACACACTGCAAGACCCTGTGAGAATAGCTATGGTTGGAAAGTACACTGGCCTTACTGATT CTTACCTTTCCGTGTTGAAGGCCCTTTTGCATGCTTCTGTTGCATGTCACAAGAAGCTTGTCATAGAGTGGGTTGCAGCT AGTGACCTTGAAGAGATAACTGCACAAGAGACGCCAGATGTCCATAAGGCTGCATGGGATCTTTTGAAGGGTGCTGATGG TATCCTAGTACCAGGAGGGTTTGGTGATAGAGGAGTGCAAGGGAAGATACTTGCTACAAAGTACGCCCGTGAAAATCAAG TCCCTTTCCTTGGCATATGCCTGGGAATGCAGCTGGCTGTTGTTGAATTTGCCCGCTCCATTCTTGGTTTTCACGATGCA AACAGCACAGAGTTTGAACCAGAAACTTCAAGCCCTTGCATCATATTTATGCCAGAAGGATCCACAACTCATATGGGGGG CACAATGCGCTTAGGGTCAAGGAAGACTTACTTCCAGGTTGCTGATTGCAAGTCTGCCAAGCTGTACGGTAATGCAAAGT TTGTAGATGAGCGACACAGGCACAGATATGAGGTAAATCCAGATATGATATCAGAAATTGAGAAGGCTGGCCTCTCCTTT GTTGGGAAAGATGAGACTGGGCGTCGTATGGAGATTGTTGAACTACCAAGTCATCCATACTTTGTGGGTGCTCAGTTCCA TCCTGAATTCAAGTCCAGACCTGGGAAACCTTCTGCATTGTTTCTAGGTCTTATCGCAGCAGCGTCTGGGTGTCTAGAAT CAGTATTGCAAACAGGTGGCAAGGTGAACATAGTTTCGAAAAATGGAGTAGCCAATGGATCTGCAATGGGGAAAGTTCAT CAGAACGGCAATGTCTATAGCAATGGAAACGGGCTTCACCACTGACTCGAGAAAGGAACAATTTTACTGCACTTTTTAAG CTTTTTTGCTTTTAATCAAAGCTTTCTTTAAGCAAGTCGACATGTATAGTCCCTGAGTTTTGTT ................................................................................ ................................................................................ .......i............................................................N........... .............................i.................................................. ...................................N............................................ .....N..........i...............N....................N..N....................... .........N...................................................................... .......................................................N........................ .................N...........N....................N............................. .................i.............................................................. ....N........................................................................... .................................i............................N................. ............N.............................N..N.................................. ................N................................N........................i..... .................N...............................i.............................. ..............................N............N.................................... .....................................N...............N......................N... ................................................................................ ................i.........N................................................N.... ................................................N.......................i....... ....i..................................................................N........ ......i....................N................i................................... ..........N................N.................................................... ................................................................................ ..........................................i...........N..........N.............. ..........N...........N......................................................... .........................................N...................... Pos Score Pred ------------------------ 168 0.706 Yes 229 0.256 - 270 0.550 Yes 356 0.385 - 406 0.307 - 417 0.679 Yes 433 0.236 - 454 0.396 - 457 0.206 - 490 0.324 - 616 0.136 - 658 0.072 - 670 0.130 - 691 0.189 - 738 0.565 Yes 805 0.241 - 914 0.638 Yes 943 0.330 - 973 0.240 - 1003 0.105 - 1006 0.316 - 1057 0.227 - 1090 0.204 - 1115 0.549 Yes 1138 0.139 - 1170 0.782 Yes 1231 0.165 - 1244 0.270 - 1318 0.277 - 1334 0.394 - 1357 0.139 - 1457 0.565 Yes 1467 0.436 - 1516 0.323 - 1569 0.384 - 1593 0.818 Yes 1605 0.729 Yes 1672 0.156 - 1687 0.521 Yes 1708 0.314 - 1725 0.654 Yes 1771 0.199 - 1788 0.372 - 1963 0.577 Yes 1975 0.320 - 1986 0.323 - 2011 0.184 - 2023 0.404 - 2122 0.074 -

Explain the output -- Processing locus 157: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAACTATATAGTATATATATATAAACCTTTCTGCAAATACAATCCTATATTTAAATACCAACATCCACAAGATAAGTAA AAACAGAAAAAAAAACTTTAAAAAATGCGTTGCCTCACAAAAACAAGATCGTTTCATTATGTCATCATTTTTTATTCCTT CTTCTTTTTACCGTTCTTGTCGTCCTCCTCCGACGATCAAAGAACCACCGTCTCCGGTCTTTTCTGTGGCGGACGTAGCA AATCCTCCGCAGATCCAAACTACATCCCAACTTTCGTCGAAGATATGCATTCACTATCTCTAAAACTAACTACACGCCGT TTCGCAACTGAATCATTAAACTCCACTACATCTATCTACGCTCTGATCCAATGCCACGACGATCTCTCACCGTCCGATTG TCAGCTCTGTTACGCGATTGCACGCACCCGCATCCCTCGTTGTCTCCCTTCTTCCTCCGCAAGAATCTTCCTCGACGGTT GTTTCCTCCGTTACGAAACTTACGAGTTTTACGATGAATCAGTCTCCGATGCGTCGGATAGTTTTTCTTGTAGCAACGAC ACCGTTTTGGATCCTCGGTTCGGGTTTCAAGTTTCGGAAACTGCGGCGAGAGTCGCGGTTAGAAAAGGAGGGTTTGGAGT CGCCGGAGAGAATGGAGTACACGCTCTTGCTCAGTGTTGGGAGAGTTTGGGGAAAGAAGATTGTAGGGTTTGTTTGGAGA AAGCTGTTAAAGAAGTGAAACGCTGCGTTTCGAGGAGAGAAGGAAGAGCTATGAATACAGGATGTTATCTTAGATACTCT GATCATAAATTCTATAATGGTGATGGACATCATAAATTTCATGTGCTTTTTAACAAAGGAGTCATCGTTGCTATTGTCTT GACAACGTCAGCATTCGTCATGCTCATCCTCTTGGCAACGTATGTCATTATGACAAAAGTATCAAAGACTAAACAAGAAA AAAGAAACTTAGGTTTGGTTTCGAGAAAATTCAACAACTCAAAGACGAAGTTCAAGTACGAGACTCTAGAGAAGGCAACA GATTACTTCAGTCACAAGAAAATGCTAGGGCAAGGAGGAAATGGCACCGTTTTCTTAGGAATCCTTCCAAATGGTAAGAA CGTGGCTGTGAAAAGATTAGTGTTTAACACAAGAGACTGGGTAGAAGAATTCTTCAACGAAGTGAATCTAATTAGTGGAA TCCAACATAAGAACCTCGTCAAGCTTCTTGGTTGTAGCATTGAAGGACCGGAGAGTCTCTTGGTCTATGAGTACGTGCCT AACAAAAGCCTCGACCAATTTCTCTTTGATGAGAGTCAAAGCAAGGTCTTAAACTGGAGCCAGAGGCTAAACATAATCCT TGGAACAGCGGAGGGACTGGCTTACCTACACGGTGGATCTCCCGTGAGGATTATTCATCGTGACATCAAAACCAGTAATG TTCTTCTAGACGACCAGCTCAATCCTAAGATCGCTGATTTCGGTTTGGCTCGATGTTTTGGTCTGGACAAAACTCATCTT AGCACCGGCATCGCAGGAACTCTAGGTTACATGGCTCCAGAGTATGTTGTTAGAGGACAGTTAACGGAGAAAGCAGACGT TTATAGCTTCGGAGTTCTTGTTCTCGAGATTGCTTGTGGAACAAGAATCAATGCTTTCGTACCAGAGACAGGTCACCTCC TACAAAGAGTCTGGAACCTTTATACACTGAATAGACTGGTCGAAGCTCTTGACCCATGTCTAAAAGATGAGTTTCTTCAA GTGCAAGGCAGCGAAGCTGAAGCTTGTAAAGTTCTTCGTGTGGGATTGTTATGCACACAAGCTTCTCCGTCGCTAAGACC GTCGATGGAAGAAGTGATCCGTATGCTAACCGAGAGAGATTACCCGATTCCATCTCCAACCAGTCCTCCCTTCTTGAGGG TTAGCTCTCTAACTACAGACCTAGAGGGCAGTAGTACTATATCTCATAGCACCAATAGTACTACGACGTTTAACACGATG GTCAAAACCGATCAAGCTTCTTATACTTCTTCAGAATCTTCTACTACTCGTACAATCTAATGATTTTTTTGCCTAGAATA TATTTCACCATTTTTTGAGGTAGGTGTATAAAAAAGTCATCTGATTGCATATAGAATTATTGAGTAAACTAATTTCCCT ................................................................................ ........................N.................................i..................... ................................................................................ ............................................N................................... ..................................................i............................. ................................................................................ .................................i..............N............................... ................................................................................ ...........N.................................................................... ..................................................i..........N.................. ................N.....N.................N....................................... ...................N.....................N.......N.............................. ................................................................................ .....................N..................N.............................N......... ................................................................................ ..................................................................i............. ............................N................................................... .............................................................................i.. ....................................................i........................... ..............................i............N.................................... ..................................................N............................. .......................................................N..........i............. ..................................................N............................. ....N.................N......................................................... .............................................................................N.. ...........................................................N.................... ............................................................................... Pos Score Pred ------------------------ 105 0.455 - 139 0.672 Yes 285 0.490 - 371 0.708 Yes 514 0.562 Yes 529 0.426 - 652 0.331 - 771 0.743 Yes 782 0.166 - 817 0.215 - 823 0.113 - 841 0.064 - 900 0.449 - 922 0.175 - 930 0.281 - 1062 0.467 - 1081 0.477 - 1111 0.266 - 1267 0.611 Yes 1309 0.346 - 1438 0.604 Yes 1493 0.600 Yes 1551 0.566 Yes 1564 0.266 - 1651 0.282 - 1736 0.448 - 1747 0.533 Yes 1811 0.385 - 1845 0.445 - 1863 0.200 - 1998 0.402 - 2060 0.262 -

Explain the output -- Processing locus 158: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTGGTGTGCTATTGTTGGAGTTGTCTCCTAAAAAAAATGCAAAGAAGAGTCAATTTTGATCATTTAAAAATATTTAATA AAAACAATTCCTTTAGTTTAATTGGTATACTGGGCCATCAATGGGACCGACCACCAAGAGTTGTGCAAGCAATCGGATTA TTTAGCGTTTTTGATGAGTTCCAGTAATCTATTTGCTTTTCTCAATAGCTTTAAAATGGTGAATTCGAATGAAGAAGACG AAAGGAGAATTCTTGACGTTGAAGAATCTCTTCTTACACAGGAGGAAGTGAACAATCACTTGACCGGATTGTCTTCTACA GCTGAAGATGGTTCTATTGACATTTATGGAAACCCACCATCGAAGAAGAAGACAGGAAACTGGAAAGCTTGTCCCTTTAT TCTTGGAAACGAATGTTGTGAGAGATTAGCTTACTATGGAATTGCCAAGAATCTAATCACTTACTACACAAGTGAATTAC ACGAGTCCAATGTTTCTGCAGCTAGTGACGTTATGATTTGGCAAGGAACTTGCTATATCACACCTCTCATTGGAGCTGTT ATAGCTGATTCTTACTGGGGAAGATACTGGACCATTGCTTCTTTCTCTGCTATTTATTTCATTGGAATGGCTTTGTTGAC ACTCTCGGCTTCACTTCCGGTTTTAAAGCCAGCGGCATGTGCTGGCGTTGCTGCGGCTCTATGCTCACCAGCAACAACGG TTCAGTATGCGGTTTTCTTTACAGGGCTTTACCTTATTGCTCTTGGTACTGGAGGTATCAAACCATGTGTATCATCTTTT GGTGCTGATCAGTTTGATGATACTGACCCGAGGGAACGAGTTAGAAAAGCTTCTTTCTTTAACTGGTTTTACTTCTCCAT CAACATTGGTTCATTTATCTCATCCACTTTGTTAGTTTGGGTTCAAGAAAATGTCGGGTGGGGACTCGGTTTCTTGATTC CCACCGTGTTTATGGGAGTCTCTATTGCGAGTTTCTTCATTGGAACTCCGCTTTATCGGTTTCAGAAACCGGGAGGTAGT CCGATTACTAGAGTTTGCCAAGTTCTTGTAGCTGCATACCGTAAATTGAAGCTTAATCTCCCTGAAGATATCTCATTTCT GTATGAAACACGAGAGAAAAACTCCATGATTGCTGGAAGCAGGAAGATTCAACACACTGACGGTTACAAGTTTCTTGACA AAGCTGCTGTTATCTCAGAATATGAATCTAAATCCGGTGCTTTTTCCAACCCATGGAAGCTATGTACTGTAACTCAAGTC GAAGAAGTTAAGACTCTAATCCGTATGTTTCCAATCTGGGCCTCGGGGATCGTCTACTCGGTCCTATATTCACAGATTTC CACTCTATTCGTGCAACAAGGACGGTCCATGAACCGAATAATCCGTTCTTTCGAGATCCCTCCTGCTTCATTCGGGGTTT TCGACACACTTATCGTGCTCATATCCATCCCAATATATGACCGTTTCCTCGTACCCTTTGTAAGACGGTTCACAGGGATA CCAAAAGGGTTAACTGATCTGCAGCGAATGGGCATTGGTCTTTTTCTCTCTGTCCTAAGCATTGCAGCTGCAGCTATTGT TGAGACAGTGCGGTTGCAGCTTGCTCAAGACTTTGTTGCAATGAGCATATTTTGGCAGATCCCTCAGTACATACTAATGG GAATAGCAGAGGTCTTTTTCTTCATTGGTCGAGTTGAGTTTTTCTATGATGAATCTCCTGATGCAATGAGAAGTGTATGC AGCGCTTTGGCACTACTCAATACCGCAGTCGGAAGCTACCTGAGCTCGTTGATCCTCACTCTTGTGGCGTATTTCACAGC TCTTGGAGGTAAAGATGGTTGGGTTCCAGACGACCTTAATAAAGGACATCTTGACTACTTCTTCTGGCTTTTGGTCTCTC TTGGACTTGTCAACATTCCTGTTTATGCCTTGATTTGTGTTAAGCACACAAAGAAGAAGGCTTTGTAAGCTATTTTTGTA ACAAGCTATGTTGTTTTGGTTCATCATATTCATGGTATACTCTGTATATTGTAATCTGTACTAGTAGATTTTCTTAAAAA TATGAAT .....................................N.......................................... ........................................N....................................... .............N.........................................i............N........... ................................................................................ .......i.................N...................................................... ............N......................N............................................ .........N......................i............................................... ..................................................................i............. ....................................i.......................N................... ......N.........................................................N............... ................N............................................................... ..................................................i............................. ...........N.................................................................... ................................................................................ ..N......................i...................................................... .....................N..............................i........i.................. ........................N....................................................... ............................N................................................... ....................................N........................................... ...........................N.................................................... ........................................N...................................i... .............................................N..N...........N....N..........N... ................................................................................ ..............N................................................................. ........................N....................................................... .......N.......................N................................................ Pos Score Pred ------------------------ 38 0.330 - 121 0.380 - 174 0.371 - 216 0.664 Yes 229 0.420 - 328 0.611 Yes 346 0.318 - 413 0.181 - 436 0.223 - 490 0.301 - 513 0.579 Yes 627 0.787 Yes 677 0.648 Yes 701 0.143 - 727 0.386 - 785 0.161 - 817 0.065 - 931 0.839 Yes 972 0.317 - 1123 0.225 - 1146 0.727 Yes 1222 0.248 - 1253 0.599 Yes 1262 0.609 Yes 1305 0.151 - 1389 0.284 - 1477 0.356 - 1548 0.459 - 1641 0.409 - 1677 0.757 Yes 1726 0.250 - 1729 0.229 - 1741 0.149 - 1746 0.452 - 1757 0.311 - 1855 0.269 - 1945 0.127 - 2008 0.076 - 2032 0.031 - 2082 0.049 -

Explain the output -- Processing locus 159: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTATTTGGAAGATCAGTGTTGGTGAATTGAGTTTCCTTTAATAGTAATGTGAAGTAGCTGTGTTTGCTTTGTTTGTGA GTGATTTGATATAAAAGTTGATTACTTTTGAATTTGTATGTAGAGGGTTTTTGTTGGATGGCACTTTGTGGTGTTTGTTC GACTCCAAATCTGCCGAATTTACAAGTGTTTCGTTCTGTTAGGAACTCGAGCATTGGTTATAAACGAAACCATAGTCTAT GGCAGCTCAGAAGTTCATCATTTCGTGCTAAATCTGTGATATTTCATTGTTCATCGTCCTTAAGACAGTCACCATCTAAT GTGGAGGAGATAGATGATAATCCTTCGGTATCATTAGAGGATGAATCAGCACATGTGATGCAGTTCAAGTGGTCTGATTT TAGGATTCTTGATCGTGTTAGCATTGGTCATGGCGGCAGGGCTGATGAGCTTGTGTTTGAAGCTATAGTTCAGGTTCCAG ATAGCCCTTTGTTTAACCAAGGAGTTGTTCTTCGGAAATTGAATACCACTCGAGCTCAAAGACGAGGAAGAAGAGCTATA GAAGTTTTTAAGAAGCTAGTTCGTCGGAGACTTCTCTACCATTCTTACTCAATGCAAGTTCACGGTTATATCACCAATAA CTTAAGCGATGATCAGTACTCATTTACCCTTGTACATGGGTGCCATGGAAGTTTCTCGATTAGGCACTGGCTTCAACAAT CTGATTGGATTCCAACATTAGAAGCTACTCTTGCATTAGATGAAGAATCCTTTCGAAGGGTGGGTGATGATACTACTGGA GGGCCTGCAGTTTCAAGGCAGTTAAGACTAATCCGTACACTAATGAGGGATATTTTGATCGGAGTCAATTACTTGCACAG CCATGGTCTGGCTCACACAGAACTGAGATTGGAAAATGTGCATATTAGCCCTGTGGATAGACATATCAAAGTAGGGATTC TCGGAAATGCTGCTGACTTTAACGGGGATGTTCCAAGTACTAGCAACGCTTACAGTACCATGGACAGACGACAAATGATG ATAGCATTTGACATGAGATGTGTTGGATTCATGATGGCAAAAATGGTACTTCAAGAATTGATGGATCCATTAATCTTTGC GAAATTGAAGTCTTTCCTGGCAAAGGGGAATGATCCTTCCTCGCTGCGGGAGTTTTTTGTGACGACGCTCAATACAAACT CTGAATCTGGAAATACCGGAGTGCAAATACTTGATAGAAACTGGGGAGCAGGTTGGCACCTTTTATCTTTGTTAATTGCT ACCAGACCTTCTGAAAGAATAAGTTGCTTGGATGCTCTTAAGCATCCCTTTCTATGTGGACCAAGATGGCGTGTTGCCCC ATCAATGGATATCATCAGATGGGGTCTTGGATCAACCGCAGTAAAGATTTCAGAAGAATACATTTACCGCATGCCTCAGC GCCAAAGACTTGCCCACTTCATTGGACTGATGGAGATGCTAAATCCTTATCCAAAGCCAAACTGTTGGTTGGAGCTTTTA CCGGGAAGATGGCGTCTTTTATACTCAACTGGAAAGCACATAGGTCTAACTCTGCGTCAGCCTTCCACACGTGCCTTAAT AGGCAACGTTCACTTAACGATAACTCGAGCTTCAGAATCCATAAACAACACTTCACTTTCCTTTACCTCTGATATACGCT TCACTGCCATAACCAGCAAAGACTGGCCACACAACAAAATCGGAGCTGCTGGGAAATTACAAACGCTCTCTCAATTCAGA CTAATAGCTGGAAAAAGACTTTACCTCAAAGAAGAGAAAAAGAACATTGGTAAGTTCTCAATGGGTGAACCAGATGCTGA AGAAGGTTTAGCCGAGAAGCTTGAAACCGAGAAATGGAAAAAAGTCGTGCCCTTCAAGGAGTTTCCGTCGAGTCTTCCTG TAGCAAAACTCGTCTCTGGAGAAATCGAAGTGACGATGAACATGAATGATCATATAGATTCACCTGGGAGTGTGATTGGA GAAGTTAGAAAGCAAATTCCGCCGGAAATGTTCGATCTTTCTAAGCTTGTGTGTGGGACTTATATAGACAGTAGGTTACT TGTACTTAGGTGTGTAAATGGTTCAGCATTGTTGTTCACAAGGTCCAGCTTGGACCATAAGTCTATGTAGCCATTTGGCT CAGGTTCTTATGTATATAACAAATGAATCATTCTTTTTGGGACTAAACCGTTGTAGTCTCTGTGTATGTTGTGGTAATGT ATGCTTTTG ................................................N............................... .....................................N...................N...................... ..............................................................................i. ..............................................................................N. .............N..........................N...........N....i...................... .............................N..............N................................... ................................................................................ ...................................................N............................ ........N..........................N........N................................... .......................................N..........................N............. ..........................................N..................................... ..N................................N............................................ ......N....................N...............................N..............N..N.. ............N....i............N..N........N.................N................... .............................N.................................................. ................................................................................ ...............................N.....................i...........i.............. ....i.............i...................................................N......... .............................N.....N............................................ ........i....................................................................... ................................................................................ ................................................................................ ............................................................i............N...... .................................i.............................................. ...................................i.....N...N.................................. ...........................N.................................................... .................N..............................................N............... .........N............N..........................................N..........N... N........ Pos Score Pred ------------------------ 49 0.306 - 118 0.201 - 138 0.379 - 239 0.707 Yes 319 0.164 - 334 0.269 - 361 0.237 - 373 0.131 - 378 0.707 Yes 430 0.400 - 445 0.118 - 612 0.304 - 649 0.442 - 676 0.362 - 685 0.279 - 760 0.241 - 787 0.141 - 843 0.408 - 883 0.255 - 916 0.202 - 967 0.309 - 988 0.228 - 1020 0.356 - 1035 0.247 - 1038 0.263 - 1053 0.420 - 1058 0.571 Yes 1071 0.283 - 1074 0.320 - 1083 0.487 - 1101 0.219 - 1150 0.404 - 1312 0.370 - 1334 0.584 Yes 1346 0.645 Yes 1365 0.606 Yes 1379 0.621 Yes 1431 0.426 - 1470 0.385 - 1476 0.197 - 1529 0.550 Yes 1821 0.659 Yes 1834 0.186 - 1874 0.709 Yes 1956 0.556 Yes 1962 0.446 - 1966 0.111 - 2028 0.265 - 2098 0.160 - 2145 0.166 - 2170 0.146 - 2183 0.145 - 2226 0.043 - 2237 0.056 - 2241 0.037 -

Explain the output -- Processing locus 160: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGGTGCTAATGGATCAGGGGTACGTCCTAGAACTTTTTTGTTTTCATCTTGATTTTGAGTTTCATCTTACTACCTGTCA GAATTTTGTCTCGTCATTTAACGTCATTTGATGGCAGTACAGATTGATCATCCAATCCTAATCACTGAATGTGCATGCAA CCCAGTTCAATCTCGTAGCAAAATGGCAGAACTGCTATTTGAGACTTATGGAGTGCCTGCAGTTGCATTTGGAGTCGATG CTGCTTTCAGCTACAAATACAATCAACTACATGGAATTTGTAAAAAAGATGGAATTGTTCTCTGTCCTGGATTCACGACA ACACACTCCATTCCGTTTGTCGACGGAGAACCTATATATAAAGGATCCAGCCGAACTAACATTGGTGGATATCATGTCAC TGATTATTTAAAGCAGCTTCTGTCACTTAAGTACCCTTTTCATTCGTCTAGGTTTACATGGGAGAAGGCCGAAGATTTGA AATTGGAACACTGTTATATCGCACCTGACTATGCTTCGGAAATTCGGTTATTCCAGGAAGGAAGAAAAGAAGCTGAAGAG AAAACAAGTTATTGGCAGCTTCCATGGATACCTCCTCCCACCGAAGTTCCTCCATCAGAAGAAGAGATTGCAAGGAAGGC AGCTATAAGAGAAAAACAAGGTCAAAGGCTGCGAGAAATGGCTGAAGCAAAGAGAGTGTCCAAGATTAATGACATGGAGA ATCAACTGATTAGCTTGCGTTTCCTTTTGAAGCAAGTTGACCAGGTTGAAGAGGATGATATTCCAACCTTTTTGTCAGAC ACCGGTTACGCGTCCAGGCAAGAGCTAGAGTCTACTATTACGAAAGTGACACAGTCGCTTAGAAAAGCAAGGGGTGAGCC GAAGAATGAACCAGCTGAGTATGAAGAAAACCCTGATTCTCTTAATAATGAAAAGTATCCACTTATGAATGTCCCCGATG ATATTCTTACTCCTGAGCAGCTTAAGGACAAGAAGAGGCAAATGTTTCTTAAAACAACAGCAGAGGGCCGGCTACGAGCT AGACAAAAGCGTAATGAGGAGGAACTCGAAAAAGAGAAAAGAAATCAATTAGAGGAGGAAAGACGTCGTGAGAACCCAGA GTCTTACTTAGAGGAGTTGCAAGCTCAGTACAAGGAAGTGTTGGAGAGAGTTGAGCAGAAGAAGCGTCTGAAAACAAACG GGTCCAGTAACGGGAATAACAAGTCTGGAGGTATTGGGCGAGGCGAGCGACTCAGTGCTGCACAGAGGGAGAGAATGCGT CTGCTGACGACAGCAGCCTTTGATAGAGGGAAAGGCGAGGATACGTTTGGTTCTAGAGATGAAGATTGGCAGCTCTACAA ACTTATGAGCAAGGATAATGACGATGATGACGAACAACCTGATTCAGACGAGGCAGAGTTGGCTCGTTTATCATCTAGAC TTCAGGAAATTGATCCAACATTTGTGCAGAAAGTAGAAGGAGAATTGAGTCAGACATCAGGGGAGGTGCCACGCGTACGC CCATTAACAGAGGAAGACTACAAGATAGTGATTGGTATAGAAAGATTCCGTTGCCCAGAGATCCTGTTCCATCCAAACCT TATTGGTATTGACCAAGTAGGCTTAGACGAGATGGCTGGCACATCAATCAGAAGGCTACCGCACGACGAGAAAGAGTTAG AGGAGAGGCTAACGAGCTCGATACTAATGACGGGCGGGTGTAGCCTTCTTCCAGGGATGAACGAGCGGTTGGAATGTGGG ATTAGGATGATAAGACCTTGCGGATCACCCATTAACGTGGTTAGAGCTATGGATCCAGTGCTGGATGCATGGCGAGGAGC ATCTGCATTTGCTGCTAATTTGAACTTCTTGGGGAATGCCTTTACTAAGATGGATTACGACGAGAAAGGTGAAGATTGGC TTAGAAATTATCAAATTCGATACAACTATTTGTGATTCCTCCAGTTGCTATTAATTGTGGAGTTTCTTATTTGATTAGCT TTAGCTGTCTCTAGATCAATGAACGATGCTTTAGGACAAAAATACCAAAATTTA .........N...................................................................... ..............................i.....................................N.....N..... ......................i........................N.............................N.. ..............................N.................N............................... .........................................................................N...... .........................................................N...................... ..............................N................................................. .......................N........................................................ .....................................i..............................N....i...... ......................................................N......................... ................................................................................ .....N..............N..........................N................i...N........N.. .........................................i...................................... .............N.................................................................. ................................................................................ ..........................................................................N..... ..........................................................N..................... ....N............N.....i..N..................................................... ................................................................................ ................................................................................ ...............................i................................................ ..........................N.............................N................i...... ......N.........................................N...............N...i........... ...................................N.............N.............................. ................................................................................ ..................N......N............................ Pos Score Pred ------------------------ 10 0.321 - 111 0.586 Yes 149 0.423 - 155 0.071 - 183 0.742 Yes 208 0.300 - 238 0.140 - 271 0.289 - 289 0.397 - 394 0.135 - 458 0.215 - 511 0.308 - 584 0.500 - 678 0.856 Yes 709 0.194 - 714 0.748 Yes 775 0.418 - 886 0.246 - 901 0.270 - 928 0.215 - 945 0.772 Yes 949 0.267 - 958 0.230 - 1002 0.666 Yes 1054 0.271 - 1275 0.445 - 1339 0.172 - 1365 0.439 - 1378 0.309 - 1384 0.596 Yes 1387 0.271 - 1632 0.596 Yes 1707 0.386 - 1737 0.115 - 1754 0.612 Yes 1767 0.196 - 1809 0.268 - 1825 0.106 - 1829 0.610 Yes 1876 0.432 - 1890 0.193 - 2019 0.052 - 2026 0.072 -

Explain the output -- Processing locus 161: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCGAAAGCCGTCATTCTTTACAACTTGTGTGACTATATAACAAAGCCACAAGGAATTGATGATTCACATGCGAAAGCAAA ATATCCATTGCAATATTCTAAAAATTTTCACACTTATCCATCCAAATGTCAAATTTGCGTTTGCGTTTACTTTCTCTCGT GTCCATCCTCTACTGCATCGCTGCTCTCCGATGTCGTCCAGACCAAACCGAAACCATTAAACGTTTCAAAAACGAGTTTG CGTTCTCTAGCATCTGCAGAAATGACACCAACTTCTTTAGCGGTGTGGTCTGCGATAACACGACCGGTGCGGTCACGGTT CTAGAACTCCCCGGTGGATGTTTACGTGGCACTCTCAGACCAAACAGTAGCCTCTTTGAGCTAAGTCATCTCCGTTACCT AAACCTCTCTTTCAACAACTTCGATTCTTCTCCCTTGTCCTCTGCTTTTGGTCAGCTCAACAATCTAGAGGTCTTGCTTC TATCCTCTAATGGCTTCACTGGCCAAGTTCCTTCTTCTATACGTAACCTAACCAAACTTACCCAATTAAATCTTCCCCAC AACAAGCTCACCGGTGATTTACCCTCACTTGTACAGAATCTAACCAAGCTTTTAGCTTTAGACCTTTCTTATAATCAATT CTCTGGAACCATACCTTCTTCTTTTTTCACCATGCCTTTCTTGTCTTATCTTGATTTAAGTGAGAATCATCTCACTGGCT CATTTGAAATCTCTAATTCTTCGTCTAAACTTGAGAACTTAAATCTTGGGAATAACCATTTTGAAACAGAGATCATAGAT CCTGTTTTGAGGTTAGTTAATCTCAGGTATCTAAGCCTCTCTTTCCTAAACACAAGCCACCCAATTGACTTATCAATTTT CTCTCCTCTCCAGTCTTTGACACACCTTGATCTTCACGGCAACAGTTTAACACTAACGAGTGTGTATTCAGACATTGACT TTCCAAAGAACATGGAGATCTTGCTCTTGTCTGGATGCAACATCAGTGAATTCCCCAGATTCTTAAAGTCCTTAAAGAAG TTATGGTATTTAGATCTTTCCAGCAACAGAATCAAAGGAAACGTTCCTGATTGGATATGGAGTCTTCCTCTTTTAGTCTC TCTGGATCTTAGTAACAATTCATTCACCGGTTTCAACGGTTCATTGGATCATGTTTTAGCCAACTCATCGGTCCAAGTAC TAGATATTGCTTTAAACTCTTTCAAAGGATCATTCCCTAATCCACCGGTCTCTATCATCAACTTGTCTGCTTGGAACAAT AGTTTCACAGGAGACATTCCTCTCTCGGTCTGTAACAGAACCTCTCTTGATGTTCTTGATCTGTCCTACAACAACTTCAC TGGCTCAATTCCTCCATGTATGGGTAATTTCACCATAGTGAATCTCCGGAAGAACAAGTTGGAAGGAAACATTCCAGACG AGTTTTATAGCGGTGCTTTGACGCAGACACTTGATGTTGGATACAATCAACTAACCGGAGAACTTCCAAGATCTCTCCTG AATTGCTCATTCATAAGGTTTCTTAGTGTGGATCACAACAGAATCAATGATTCATTTCCCTTGTGGCTCAAGGCATTGCC CAATTTGAAAGTCCTTACTCTCCGTTCAAACAGTTTCCATGGTCCTATGTCTCCACCAGATGATCAAAGTTCTCTTGCAT TTCCCAAGCTCCAGATACTTGAAATATCGCATAACAGATTTACGGGAAGCTTACCAACGAATTACTTTGCGAATTGGAGT GTAAAATCTCTCAAGATGTATGATGAAGAGAGGCTATATATGGGAGACTATTCGAGTGATCGGTTTGTTTATGAAGATAC TTTGGATTTGCAATACAAAGGTCTATACATGGAGCAAGGCAAGGTTCTTACTTTCTACTCCGCCATTGATTTCTCTGGCA ACAAGCTTGAAGGAGAGATTCCAGAATCTATTGGTCTTTTGAAAACATTGATTGCTCTCAACTTATCAAACAACTCATTC ACTGGACATATTCCGATGTCTTTCGCTAATGTGACAGAGCTCGAGTCATTGGACTTGTCAGGGAACAAACTCTCGGGGGA AATTCCACAAGAACTCGGAAGACTATCGTATTTAGCGTACATAGATGTCTCTGATAATCAACTCACTGGTAAAATACCAC AAGGAACACAAATCATAGGGCAGCCTAAATCCTCATTTGAAGGAAATTCAGGGTTATGTGGTCTTCCTCTTGAAGAAAGC TGTTTAAGAGAAGACGCACCATCGACACAAGAGCCCGAAGAAGAAGAAGAAGAAATATTGGAGTGGAGAGCAGCGGCAAT AGGGTATGGACCAGGGGTGTTGTTTGGATTGGCAATTGGACATGTTGTTGCTTTGTACAAGCCAGGGTGGTTCATCAAGA ATAATGGTCAGAATAGGCTTAGAGGCATCAGACACCCTTAGGATTAAAATTGTTCTGTTTCATTTTTTTTTTGTAAATTG ATCAATGAGTAAGTAAAGTTACTCAAAAACAGAATTATGGCATGTTCTGTTTACATTTAA ..........................................................N........N............ .............................................N.................................. ..............................N................................................. .....................i.......................................................... .................N.............................................................. ................................................................................ .........N...................................................................... ................................................................................ ...............................N................................................ ................................................................................ ................................................................................ ................................................................................ ...........N......................N............................................. ..N.....................................................N....................... ..................................................N............................. ................................................................................ .................................................i.............................. ...............N...i............................................................ .................................N.............................................. ..............................................N................................. ......................................N.......N............N.................... ................................................................................ ...............i...N..N................N..............................N......... ............................N................................................... ................................................................................ ...............i............N................................................... ............................................N................................... .......................................................i........................ ................................................................................ .....i...................................N...................................... ...N............................................................................ ....N...............................N....N.................. Pos Score Pred ------------------------ 59 0.302 - 68 0.361 - 126 0.347 - 191 0.322 - 262 0.709 Yes 338 0.115 - 490 0.472 - 672 0.442 - 972 0.302 - 995 0.195 - 1043 0.143 - 1097 0.276 - 1171 0.242 - 1330 0.550 Yes 1376 0.309 - 1380 0.595 Yes 1474 0.306 - 1567 0.386 - 1639 0.262 - 1647 0.462 - 1660 0.233 - 1776 0.681 Yes 1780 0.342 - 1783 0.410 - 1800 0.431 - 1831 0.109 - 1869 0.460 - 2016 0.682 Yes 2029 0.282 - 2125 0.385 - 2216 0.580 Yes 2326 0.712 Yes 2362 0.287 - 2404 0.394 - 2485 0.047 - 2517 0.066 - 2522 0.065 -

Explain the output -- Processing locus 162: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCACAGAAAAACCTGGGAAGAAGAAGTAAGAGTGCTTGTCTGCTTCGTATTTGCTCAACCAATCAGGAATGCCTCAAGTC ATTCTTATATTCGAAGGCTAAATGCTGGATTTTCTCGTGCAGGCACACATTGAGCGGGTGTTGCGTGACCATTTCCTCTT CAGGAAACTCACTGATTCTCAGTGCCAGGTTTTGTTGGACTGCATGCAAAGGCTTGAAGCTAATCCAGGGGATATTGTTG TGAAACAGGGTGGTGAAGGGGACTGCTTCTACGTTGTGGGTAGTGGAGAATTCGAGGTCTTAGCAACTCAGGATGGAAAG AACGGTGAGGTACCTAGGATCTTGCAGCGTTATACAGCTGAGAAACAATCATCATTCGGTGAACTCGCCTTAATGCATAA CAAGCCGCTTCAGGCTTCTGTACGTGCTGTAGATCACGGAACATTGTGGGCCTTAAAAAGAGAAGATTTTCGAGGAATTT TGATGTCTGAGTTTTCAAACTTAGCATCCTTGAAGCTTCTTCGTTCTGTCGATCTTCTTTCCCGTCTTACAATTTTGCAA CTAAGCCATGTTGCAGAGTCTCTTTCCGAAGCTTGCTTTTCTGATGGACAAACAATTGTTACCAAGGACCAAAAACTTCA GGGCCTGTATGTTATCCAGAAGGGACGCGTAAAAATATCTTTCTGTACAGAGGTGTTGGAGAGTCAAAATGTTTCAAGCC TTACAACTGGAATCACTAACGAGTATGACAATCTTGAAATCGGAACAGAAGTCTCCATAGAAAAGCATGAAGGAAGTTAC TTTGGTGAATGGGCTCTTCTTGGCGAACTCAAAGATTCCTTAAGTGTGGTTGCAGTTGGCGAAGTGGTTTGTGTGGTTTT AACAAAAGAAAATTTCGAGTCAGCGGTCGGCCCTCTGACCAATATTTCAGATGACGGTCCCAAGACAAGACATTCTTCAT TCGAGCTGTCAAAGGAATCTGCAAAAGTTACCGATACCACAGCTCTTGCTAAAGCCACTCTTGCAGACCTGGAATGGACA ACATGCTTGAGTACAACAGACTGTAGTGAGATTGGGCTCGTGCATTTGAAAGATAAAGAAAATTTGCTCAGCTTGAAAAG ATTTTCAAAGCAAAAGGTGAAAAAGTTAGGTAAAGAGGCACAAGTATTGAAAGAGCGGAATCTGATGAAGAACGTAATAA AGCCCTCAGCTATTGTTCCCGAAATCTTGTGTACTTGTGTCGATCAAACATTTGCGGCAATCTTACTGAACACTACTCTT GCCTGTCCTATCTCTTCTCTGCTTCACTCTCCGCTTGACGAGTCATCTGTCCGTTTCATTACCGGCTCACTTGTGTCTGC CATAGAAGACATACACAAGAACGAGATTCTCTTCAGAGGTTCATCCCCCGAGTTACTGATGTTGGATCAATCCGGATATC TACAGATTGTAGACTTCAGATTCGCCAAGAAATTGTCCGGGGAACGAACATTTACAATCTGTGGAAATGCAGATTATTTA GCCCCAGAAATTGTTCAAGGCAAAGGCCATGGTTATGCAGCTGACTGGTGGGCTCTTGGAGTTTTGATCTACTATATGCT AGAAGGAGAAATGCCATTCGGGTCATGGAGAGAGAGCGAGCTCGACACGTTTCAGAAGATTGCAAAAGGCCAACTAACTT TCCCTCGAGTTCTAAGCTCAGAAGCTGAAGATCTCATCACAAAGTTGCTTGAAGTTGATGAAAACCTGCGGTTTGGTAGC CAAGGAGGTCCAGAATCAATCAAGAAACATCCCTGGTTCAATGGTCTTAAATGGGAAGCTATTAGTAACCGCGAATTTCA AGTTCCTCAAGAGATAATCTCGCGAATTCATCACCATTTGGAGAATGATAACGTTCTCCCTCTAGAAACTTCTAAATCGC TTGACACAACAGAAGATCAAGATGCTCAAAACTGGCTTGAAGAATGGTAAGACACTTCAGACGAAACCGCGGCTCTTTTA TCTCCGGTTCTATTCAATTAAAATTTCGATACTTTTAAACAGTCATTGATGCTAAAAACAAGAGAGCCT ....................................................................i........... .....................N.......................................................... ...........................................i.................................... ........................................................................N....... ........................................................................N....... ................................................................................ ..N............................................................................. .......N...................................N.................................... ........N...........................................................N........... ........................N.........................................N............. ........N....................................................................... ..................................................N............................. .........................................................................i...... ..i............................................................................. ................................................................N............... ................................................................................ ................................................................................ ..........................................................N..................... ..................................................................N............. ............................N.....N........................................N.... ..........i.............N....................................................... .........................................................N...................... ........................................N.........N............................. ............................................i................................... .....................N.....................N.................................... ................................................N.................... Pos Score Pred ------------------------ 69 0.613 Yes 102 0.433 - 204 0.548 Yes 313 0.255 - 393 0.241 - 483 0.455 - 568 0.355 - 604 0.210 - 649 0.189 - 709 0.376 - 745 0.214 - 787 0.330 - 809 0.262 - 931 0.169 - 1034 0.736 Yes 1043 0.640 Yes 1185 0.328 - 1419 0.378 - 1507 0.498 - 1549 0.424 - 1555 0.293 - 1596 0.301 - 1611 0.642 Yes 1625 0.295 - 1738 0.208 - 1801 0.266 - 1811 0.292 - 1885 0.500 - 1942 0.229 - 1964 0.487 - 2049 0.045 -

Explain the output -- Processing locus 163: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGTGGTCCTTTTGAATACTACATTTTCAATTAAATATGACTATAATATTACAGCTATCAGGCATAATATATATTGAAA TTGACACATACATATATATAGTTTTATACAGAACTCATGACATTTTTATTCTTAAATAGCTCCTTCTATATAATAATTTA TAGAGCCTTCCACATCTCCTTTGTTCCCTTAACAAACTCTAAACCAAAAAAAAAAAAAAAAACTATGGGTTACGACAGGC TCGGTCCATCCGGACCATCAAACCCGAACCAGAAGGACCCTGCCACGTCACTCCCGGAACTACAGAAGAAAACTAAAACG AAGCTGATTCTCTTCACTTTGGCCGTTTTGGTAGTTGGAGTAGTCTGCTTCGGTATCTTTGCCGGCATCCGAGCCGTAGA CTCGGGTAAAACCGAGCCGAAACTAACCCGTAAACCGACCCAAGCAATCTCCCGAACCTGTAGCAAATCCTTATACCCGA ACCTATGCATCGACACACTTCTTGACTTTCCGGGATCTTTAACCGCCGACGAGAACGAGCTCATTCACATATCATTCAAC GCAACGCTTCAAAAATTCAGCAAAGCTCTTTATACTTCTTCGACGATCACATACACTCAGATGCCCCCACGTGTACGGTC AGCTTACGATTCTTGCCTTGAGTTACTTGATGATTCAGTGGACGCGCTCACACGCGCTCTTTCCTCCGTCGTTGTCGTCT CCGGAGATGAGTCTCATTCCGATGTGATGACGTGGCTTAGCTCTGCGATGACTAACCACGACACTTGCACTGACGGATTC GATGAAATTGAAGGTCAAGGAGGAGAAGTGAAGGATCAAGTGATCGGAGCGGTTAAAGATTTGTCGGAGATGGTGAGTAA TTGTTTGGCTATATTCGCCGGAAAAGTTAAGGATTTATCTGGAGTTCCGGTGGTGAATAATAGGAAGTTACTTGGGACTG AAGAAACAGAGGAATTACCTAATTGGTTGAAGAGAGAAGACAGAGAGCTTCTTGGTACTCCAACGTCGGCGATTCAAGCT GATATCACGGTGTCGAAAGACGGTAGCGGAACGTTTAAGACGATCGCGGAGGCGATCAAGAAAGCCCCGGAGCATAGTAG CCGACGATTCGTCATCTACGTTAAAGCAGGAAGATACGAAGAAGAGAATCTGAAAGTTGGTAGGAAGAAGACAAACTTGA TGTTCATCGGGGACGGCAAGGGTAAAACGGTCATAACCGGTGGGAAAAGTATCGCCGATGATCTAACTACTTTTCACACC GCCACTTTCGCTGCGACTGGTGCTGGATTTATAGTGAGGGACATGACGTTTGAGAATTACGCCGGACCGGCTAAGCATCA GGCCGTGGCACTCCGTGTTGGTGGAGACCACGCGGTGGTTTACCGTTGCAACATTATCGGTTACCAAGACGCGCTTTACG TACATTCTAACCGACAGTTTTTCCGCGAATGCGAAATTTATGGAACGGTCGATTTTATATTCGGGAATGCGGCTGTGATC TTACAGAGTTGTAACATTTATGCGCGTAAACCAATGGCTCAGCAGAAGATTACTATTACGGCTCAGAACCGAAAAGATCC GAATCAGAATACGGGGATTTCGATTCATGCTTGTAAGCTACTAGCAACACCGGATCTTGAAGCCTCTAAGGGTAGTTATC CGACGTATCTCGGCCGTCCGTGGAAGTTGTATTCTAGAGTTGTGTACATGATGTCGGATATGGGTGACCATATTGACCCG CGAGGATGGTTGGAGTGGAATGGTCCGTTTGCATTGGACTCGTTGTACTATGGTGAGTATATGAACAAAGGGTTGGGTTC AGGAATTGGTCAACGAGTCAAATGGCCTGGTTATCATGTTATTACCTCAACGGTAGAGGCTAGTAAGTTTACGGTGGCTC AGTTCATTTCTGGTTCTTCGTGGTTGCCATCCACCGGTGTGTCCTTCTTCTCCGGGTTGTCACAATAGATTGTCTCAGCC TTGTATGTTACGTATACGAATGATTTTATGTACAAACTCAAGATCTGTAATATAGTCCATAAGCCATTTTAAGTGTACTT ATTATAT .....................................N.......................................... ....................................N........................................... ................................................................i............... ................................................................................ ................................................................................ ................................................................................ ....N........................................................................... ............................................................i................... .............................N.................................................. ......N..............N....i....................i................................ ................................................................................ ................................................................................ ................................................................................ ...............................................................................i .........................................................N...................... ..........................................i..................................... ................................................................................ ............................N..........N..........................N............. ...................N.............i.............................................. ..........................N..................................................... ...............................................i..i........N.................... .....i.............i.............................N..........i................... .....................N.............N............................................ ................................................................................ ....N..............N.......N.................................................... ....... Pos Score Pred ------------------------ 38 0.443 - 117 0.467 - 225 0.841 Yes 485 0.497 - 621 0.745 Yes 670 0.137 - 727 0.232 - 742 0.365 - 747 0.900 Yes 768 0.631 Yes 802 0.276 - 870 0.609 Yes 1200 0.687 Yes 1258 0.124 - 1323 0.695 Yes 1469 0.345 - 1480 0.321 - 1507 0.203 - 1540 0.135 - 1554 0.767 Yes 1627 0.236 - 1728 0.631 Yes 1731 0.523 Yes 1740 0.496 - 1766 0.662 Yes 1780 0.614 Yes 1810 0.271 - 1821 0.525 Yes 1862 0.429 - 1876 0.084 - 2005 0.071 - 2020 0.069 - 2028 0.036 -

Explain the output -- Processing locus 164: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCTGTGTTAGTGATTTTGACGCTATTTGAATCGAATACTGTTGATTTATATAGTTGCTTAGGGGAAGAATCGGTTCAGT ATATAGGTGCTTTGTTTATAGGAATTTGTGTTTTGTGAATGTATGTTGCAGTCGAATTCAATGGAGCTTGTGAAACAAGA TGGGAATGATTCTCTCGATATGCTTATCAGACGTGCGGTTGGAAAGGATCCGTTTCTTTCCTTCCCTAGACCTGAAAATA CTCCTGTCCAGCTTTTTCAACTTCTCCATACCTTAGAACGTCCAGGCTGGCCATTGCTGACCCCTTTGAAGATACAAATG CAGAAGTGTGAAAAGTGCAGTAGGGAGTTTTGCTCTCCTGTGAACTTTAGAAGACATAACCGCATGCATCGCCGTCAAAG AAAGCCGGAAAAGGATTTTGGCAAAGAGAGGGATGCGCTGGGAGCCTTTTGGAATAAGCTCTCTGCAACTGATGCAAAGG AGATTCTCTCAGTAAAGAGCATGATGCTTGAGGATATTCCTGGGGCATCAGTGGAGTCAGGATTAATGTCACTTATCGAA AAACCAGGCTATACTGCTCTGCCACAATATTATCTAAGAGCTGGTTCTGGCCTTTTGGATCTTCTCCAGGCTAGACCACC TAGACTTCCAATTTCTTCGCAGGAACTTTTTAGCATTCTTGATGATGCAAGTGAAAAAACTTTCCTGTCTAGCGAGGCTG CACCAATGCAAAAGTACATTTTTGACGGGGAAATAGGAAAAACTGTACTGGAGGCAAAAAATGTTGTTGCTTGCGCAAGC TTCCTTCTGGAACAGCGATTGATTAAAGCATGGCTTGCTGACAAAGATGCTGAAGCTCTGAGGTGCCAAAATTTACTGGT TGAAGAGGAAGAAGCTGCTCGAAGAAGAAAAGCTGAGCTCTTGGAGAGGAAGAAGAGGAAGAAGCTAAGGCAGAAAGAGC AGAGAGAGAAGGACCAGAAAAAAGATGCTAAGGAAGACGAGAGCACCACATCAGAGGAACAACAATATCCAGCTGAGCCA TCAAGCCCTTTATCTGTAGCTTCTGATTCTGAGGCACAGACACCAGATTCCTTACCAATTGATGATTCTTCATCCCTCGA GGAACCTCAAGTTTTGGAAACTAATAATGGAAGAAACAGTGAAACGCAAGTGCCAATGGTTGATGGCTTAGACAATGGCC AAAACATGGAAAGGCGAAGTGGCCGTAGGCAAATGCAAAGATCACAACAGGGAATGCCGAATGGGTTCCATGCTGATCAT GCACCAAACCTTGGAGGCATGCGAAAGAATGGAACCAACAGAGACGCAAGAGCCAATACTACTAAAGTTTGGAGCCGAAA ATCCGATAACCCCAAATTGATATCACAACATGCAGCAGTGACTCAGCAGGATCAGACAAAGAGCAGCGAATTTATAGTTG GATCGTTGAGTGTATCAATCCGAAACAGTGGCGAACATAACCAAACCAAATGCAGTGAAGGGGAGAGAAGAACAAAGACT GTTGAGGTGAAACCCGCATCTGAGCAATCAACTGTAAAAATATGGAGACCAGTGAGCAGTCAGGGAAGAAAAACCTCAAC AGTCAATGGAAACACAGACAAAGAAGATAAGAGATCAAACCCAACTACACCCGAAGTGAAAAACGCTCATCACATAAGCT TGCAGTTTAACAACCACGAAGCAAAGGCGTTTCTTGCAAAGAGATGGAAAGAGGCAACCTCTGCAGAACACGTGACATTG GTTCTGTCTCAGGAAACCGACATCTCAGGCAACAACACTCACGAGTCCTCAAATGGTGTTATAACCGCAAGATCAAAGCT GAGGACGAAAGCCGAAAAGGGCACAAAGGTAAAGTATGTTCCAAAACAGAGGATTCCTTGAAAACTTGAAAACAAAAAGA CAAGCCTGATTCTGGACCAGAGTATCATTCTTTTTAGGTATACCAAAAGTGCAGTGTTAGCGAGCGAGACAGATATTTTC ................................................................................ ......................................N...N.................i..................N .....N.............N............................................................ .............................................................................N.. ...............................................................i................ ................................N......................................N........ ....................N..N.........................................i.............. ................................................................................ .........................................N..N................................... .....N......................................................N................... .............................N................N................................. ................................................................................ ........................N....................................................... .............................................................N.................. ..........................i............................i......i...........i..... .....i..........................i....................i......i........N........N. ..................N.........i................................................... .............................N.................................................. .................................................N.............................. .........................................N...................................... .....N.......................................................................... ...........................................N.................................... ....................................................N........................... ...................................N............................................ ................................................................................ Pos Score Pred ------------------------ 119 0.295 - 123 0.404 - 141 0.544 Yes 160 0.271 - 166 0.247 - 180 0.408 - 318 0.361 - 384 0.656 Yes 433 0.268 - 472 0.104 - 501 0.466 - 504 0.344 - 546 0.560 Yes 682 0.279 - 685 0.425 - 726 0.442 - 781 0.215 - 830 0.452 - 847 0.355 - 985 0.330 - 1102 0.404 - 1147 0.740 Yes 1176 0.680 Yes 1183 0.609 Yes 1195 0.680 Yes 1206 0.640 Yes 1233 0.563 Yes 1254 0.782 Yes 1261 0.580 Yes 1270 0.302 - 1279 0.151 - 1299 0.444 - 1309 0.589 Yes 1390 0.264 - 1490 0.419 - 1562 0.291 - 1606 0.431 - 1724 0.470 - 1813 0.198 - 1876 0.315 -

Explain the output -- Processing locus 165: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAGAGTTTAAATTTGATTTGAAGAGGATGCAAAAAACTCAGGAGAAGAAGGTTGGTTGCTTCTTATTCATCGGAGGACC TTATGATTCCTTTGTTCTTGACATACGATTTTTGATAATTTATTTGCTAATGCTATGTTTCAGAACATGAAAGAGATGGA GTTTTTCACTGAGTATGGTGATGCTAATCGTTATCGGATTCTTGAAGTTATTGGGAAAGGAAGCTATGGAGTTGTATGTG CAGCTATTGATACACAAACAGGAGAGAAAGTTGCTATTAAGAAGATTAATGATGTTTTTGAGCATGTCTCTGATGCTCTT CGTATTCTCCGTGAGGTTAAGCTTCTACGGCTTCTTAGGCACCCTGATATAGTTGAAATCAAAAGTATCATGCTTCCGCC TTCTAAGAGGGAGTTTAAAGATATATATGTTGTTTTTGAGCTCATGGAATCAGATCTTCATCAAGTCATCAAAGCTAATG ATGATTTGACTCGTGAGCATCACCAGTTTTTTCTTTATCAGATGCTTCGTGCTTTGAAGTATATGCATACAGCTAATGTT TACCATCGTGATCTTAAACCCAAGAACATACTGGCAAATGCAAACTGCAAGTTGAAAGTCTGTGACTTTGGACTTGCAAG AGTGTCGTTTAATGATACACCTACGACAGTCTTTTGGACGGATTATGTTGCCACAAGATGGTACAGGGCACCTGAGCTCT GTGGATCATTCTGTTCTAAGTATACTCCAGCAATTGACATTTGGAGCATTGGCTGCATCTTCGCTGAGGTATTAACAGGG AAGCCTTTGTTTCCAGGAAAAAGTGTTGTCCATCAGTTAGACTTGATTACCGATCTTCTTGGCACACCAAAATCAGAGAC CATTGCTGGGGTGCGAAATGAAAAGGCTAGAAAATATTTGAACGAAATGAGGAAGAAAAATCTTGTCCCCTTTTCGCAAA AGTTTCCTAACGCAGATCCTTTAGCATTGCGTCTTCTGCAAAGACTGTTGGCTTTTGATCCAAAGGATAGGCCGACTGCT GCAGAGGCGCTGGCTGATCCTTATTTTAAGTGCCTTGCTAAGGTTGAAAGAGAACCCTCTTGTCAGCCGATTTCGAAGAT GGAATTTGAGTTTGAAAGAAGAAGGTTGACAAAGGATGACATTAGAGAACTAATATACAGGGAGATACTGGAATACCATC CACAGCTGCTCAAGGATTATATGAACTCTGAAGGCTCAAGTTTTCTATATCCTAGTGCCATCGGTCATTTGAGGAAACAA TTTGCGTACCTAGAGGAAAATAGTGGCAAAAGTGGGCCAGTCATACCTCCAGACAGGAAGCATGCTTCACTTCCACGATC TGCAGTTCATTCCAGTGCAGTAAACTCCAACGCTCAACCCAGTTTAAACGCATCAGACAGTCGACGTGTTTCTATCGAAC CTTCAAGAAATGGAGTGGTTCCATCAACTTCAGCATATTCAACTAAACCTTTAGGGCCTCCACCAAGAGTACCATCAGGT AAACCAGGCCGTGTTGTGGAATCATCTGTAACTTATGAAAATGACAGAAACCTCAAAGAGTCGTCCTATGATGCAAGGAC ATCATATTACAGAAGCACAGTACTCCCTCCACAGACCGTATCTCCTAACTGTTACTTCCTTCCAAACACCATGAATCAAG AGAAGCGCAGTGGTACAGAAGCTGCATCTCAGCCAAAACCGCAGTTTGTCCCCACCCAATGCAACTCTGCCAAACCAGCT GAGCTGAACCCAAACCCTTATGTCCAATCACAACACAAGGTGGGTATTGATGCTAAGCTGTTGCATGCACAATCCCAATA TGGTCCTGCTGGAGCCGCCGCGGTTGCAGTAGCAGCACACCGGAACATTGGAGCTGTTGGGTATGGCATGTCTTAGAATT TAGCACATTGGCGCTGTTGGCTATTGCCAGAAACTCCTCTTGTTATAGTCGTTATGTAATGTAAGTATTATTCGATCAGG CCTGATTTGAACACA ...........................N.................................................... ..N..............................................N....i...........i........i.... ..............N.....N............................................N.........N.... ................................................N..N...........N........N....... .....................................................................i.......... ..........................N................N.................................N.. N........................................N....................N............N.... .....................................N.......................................... ...........N................................N............N...................... ................................................................................ ................................................................................ .................N............................i................................. ................................................................................ ..............................................................................i. ...................................N............................................ ....................N........................................................... .............................................................N.................. ................................................................................ .........i...................................................................... ..................................N.....i..........................N..N......... ......................................................................i......... ..........................................................N..................... ...................N.............................N..............N..............N ..............................................................N....N............ .....................................................N....N..................... ............... Pos Score Pred ------------------------ 28 0.145 - 83 0.102 - 130 0.166 - 135 0.673 Yes 147 0.572 Yes 156 0.804 Yes 175 0.415 - 181 0.133 - 226 0.082 - 236 0.175 - 289 0.089 - 292 0.230 - 304 0.188 - 313 0.104 - 390 0.535 Yes 427 0.175 - 444 0.334 - 478 0.130 - 481 0.259 - 522 0.406 - 543 0.279 - 556 0.235 - 598 0.236 - 652 0.298 - 685 0.248 - 698 0.418 - 898 0.227 - 927 0.635 Yes 1119 0.572 Yes 1156 0.129 - 1221 0.405 - 1342 0.445 - 1450 0.540 Yes 1555 0.284 - 1561 0.623 Yes 1588 0.348 - 1591 0.208 - 1671 0.770 Yes 1739 0.499 - 1780 0.455 - 1810 0.386 - 1825 0.263 - 1840 0.498 - 1903 0.308 - 1908 0.266 - 1974 0.039 - 1979 0.056 -

Explain the output -- Processing locus 166: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTACTCTTGAAATCTGCTGCTGTGATATCCAGAGGAACCGCCATGACGATTCGAGTTCCCAAGCTAGTCTCAGCAAAGA CAGTAACTTCTTCTGCAATAGCCATTGTGTGAGAGAGCAGTGGCGAGAATTTGGTTCCTGTTGTTGTTCATCGGGAAAAT AAAATACATCACGCTGTTTCAAGTCATGTTAAGACAAATGTTTGCAGTATTTATTAACCTAGAAACGGCAGGCTGTGCAA GTGTATATTTCGAAAGACTGAAATTCACAAAACGGTACCGTTTCCTCGGAATCATCATCTTTCCCTCTTTCCTTCCACCG AGAGCAAAACCTAAAAATGGTCGTTTGATTCTCTCTCTCTCTCTCCGTTCAAAAATGCGCCGGCACAAGCGATGGCCTTT GAGGTCTCTTGTCTGCAGCTTCAGCTCCTCTGCGGCGGAGACAGTTACTACTTCAACGGCAGCTTCAGCGACGGCGGCTT TTCCGTTGAAGCATGTGACAAGGTCGAACTTCGAGACGACGCTGAATGATCTACGCTCACTCGTAAAGGCTGCAGATTTC GTGGCGATTGACCTTGAGATGACTGGCGTGACAAGCGCTCCGTGGCGAGACTCCTTAGAGTTCGACCGCTACGACGTCAG ATACCTCAAAGTCAAAGACTCCGCCGAGAAATTCGCCGTCGTTCAGTTTGGTGTCTGTCCCTTTCGTTGGGATTCTCGTA CTCAGTCATTCGTGTCCTACCCGCACAATTTCTTTGTATTTCCTCGTCAAGAACTCACATTTGATCCACCAGCTCATGAA TTTCTCTGTCAGACTACATCAATGGATTTTCTTGCCAAGTATCAGTTTGATTTCAACACCTGCATACATGAAGGAATATC TTATTTGTCCAGACGAGAAGAGGAAGAGGCAAGTAAGCGTTTGAAAATGCTACACGGCGAGGACGGAATTGATTCATCGG GTGAAACTGAAGAGTTAAAGTTGGTGCGATTGGCAGATGTTCTTTTCGCTGCAAGGATGGAGAAATTGCTGAATGAATGG CGGTCTGGCCTGTTACATGGTGGGAATGCGTCCTCTGAGTTTCCGAGGATTTCAAATGGTTCAAACCAGAGTATGGAAAC TGTGTTTCACCATATGCGTCCTGCTCTCAGTCTGAAGGGTTTCACTTCTCATCAGCTCAGAGTTCTTAACTCGGTTTTAA GAAAGCATTTTGGAGATCTTGTGTACATACATTCAAATGATAAAAGTTCTTCTTCACGAGACATTGTTGTGTATACTGAT TCAGATTCCGACAAGGAAAACCTCATGAAGGAGGCAAAGGACGAACGCAAGAGATTGGCAGAAAGAAAAATACAATCTGC AATCGGGTTCCGTCAAGTGATTGATCTGCTTGCTTCAGAGAAAAAGCTGATCGTTGGTCACAATTGTTTCCTCGATATTG CACATGTATACAGCAAATTTGTGGGTCCTCTTCCTTCAACAGCTGAGAAATTCGTGGCCTCCATTAACAGTCACTTTCCT TATATTGTTGACACCAAAATACTCTTAAACGTGAATCCAATGTTGCATCAGCGGATGAAAAAGTCCAGTACATCACTGTC TTCCGCGTTTTCCTCGTTATGTCCGCAAATCGAGTTTTCTTCAAGAAGCTCTGACTCGTTTCTTCAGCAGCGTGTCAATA TTGACGTTGAAATAGACAACGTTAGGTGTTCTAACTGGAATGCAGGAGGAAAACACGAAGCCGGTTATGATGCTTTCATG ACAGGTTGCATCTTTGCGCAGGCATGCAATCATCTCGGTTTTGACTTCAAACAGCATTCGCAGTTAGATGACTTTGCCCA GAACGAGAAACTTGAAAAGTACATCAACCGTCTTTATCTCAGCTGGACAAGAGGTGATATCATCGACCTTCGAACAGGCC ATAGTAATGCAGATAACTGGCGAGTCTCAAAGTTCAAATACGAGAACATTGTCCTCATCTGGAACTTCCCACGAAAACTT AAGGCGAGAGGGATCAAAGAATGCATATGCAAAGCCTTTGGCTCTGCTTCTGTCACCTCTGTCTACCACGTGGACACTCT GCGGTTTTTGTCCTGTTTAAGAACTCAGAACTTGTCTGGGACTTCTTAGCACTCAAGCGGCAGCTGGAGTCAAGCGACGG TCCAGTCTCAGTTCTTCACCCTTTATCCAAGATCCTTGAAGGAGGAAACACTGGAGCTGCAGACTACG ...........................................i.................................... ................................................................................ .........................N...........N.......................................... ................................................................................ ................i.....................................i................i........ ................................................................................ ............N................................N.................................. ..................i............................................................. ................................................................................ ...........................................................................N.... .....................i.............................................N............ ..............................................N................................. ....................................N...................i...............N...N... ................N........N.............................N................N....... .............N.................................................................. ....................................N........................................... ........................i....................................................... ................................................................................ ...N............................................................................ .......................................N..............N......................... ..................N............................................................. .......................................N..........................N..N.......i.. .......................N...........................................N............ ................................................................................ ......N......................................................................... ....................i.....N..................................................... ................................................................................ .................................................................... Pos Score Pred ------------------------ 44 0.596 Yes 186 0.222 - 198 0.236 - 337 0.814 Yes 375 0.826 Yes 392 0.888 Yes 493 0.255 - 526 0.297 - 579 0.735 Yes 796 0.348 - 822 0.542 Yes 868 0.427 - 927 0.421 - 997 0.202 - 1017 0.538 Yes 1033 0.338 - 1037 0.440 - 1057 0.245 - 1066 0.123 - 1096 0.112 - 1113 0.312 - 1134 0.476 - 1237 0.368 - 1305 0.583 Yes 1444 0.236 - 1560 0.309 - 1575 0.257 - 1619 0.227 - 1720 0.395 - 1747 0.314 - 1750 0.205 - 1758 0.658 Yes 1784 0.292 - 1828 0.487 - 1927 0.276 - 2021 0.569 Yes 2027 0.336 -

Explain the output -- Processing locus 167: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACATATATTCTGGGGTTTAGCAATGGTCTTCTTAGTAGAGAACGGAGAAGGACATTTGCAGTCTGTACAGTCTCCACCA TTGGACTTACCCCAATGTTAAGACTGTTATCTACACACATATACACTTGTATATTCTCAAATTTGTTTCACAAAACATGT CATTCATTTTCAATTACAAAAGATAAACGTATCAAGATATTTTAAGTCCAAAAGTATTTTTCAATTTTGCATGCAAAAAA AAAAGCAAAACTATGCACCAGCCGGGAATCGAACCCGGGTCTGTACCGTGGCAGGGTACTATTCTACCACTAGACCACTG GTGCTTGTATGTCCCAAGAGGAAAATACACTTATATTACCAAACATATAATAATGCTTGTAGTTTCGTTCAGTTAAATCT CACTGAGCACTTTTCAAGTAGAAGAGACACAAACATCGTTACCTTCGCCGCCATGGCCTCCGCACTCGAATGCTGGACAA CACGTAATGCCGCCGGTGGTTGCGCCGACGACGATTTTGTTGACCAAGTCCTAATGTCCTCCGAAGACAGGTCTGAGTCC CTCACTGCTCCTCCACCTTCCGATCAAACCTCTTCCGCTATGCAAAAGCGGTTTCAGCGTCTCGGCCGCAACGTCTCCGA CGCGATCGCGTCGCTCAAGAACTCTCTCAACCTCGATTCGAGTCGAGACAACCAAAATGCAGCTACCGGAGGAGGTAGAA AGCTCGTATGGGCTACTGTAGTGAGAAACCTTGCTAAAATGTATCCTGGAAGCCAGTTGCCTGATAAACTCGTCTCCAAT CTCCGAAAGCATTACGATTCTTTGCCTCTCAGTTATAGTCAGACTGGTTTCGATATGAAAGATGTATTTGTACACATAAA GTTGATAGAGCAAGCTTTTGGAGATGATAATCCTGTGTTTGTGATTCAAGAGGTGTGTGATGAGGAAGCTGATGATCAGG GTTCTGTGTTTAAGCTCACATTTGCTTGTACTTGTTCCCTTCCATGGTCAACCATTTCAGGGTCTCTTGATGGTGCTTTG ATTTGTTGTAAGAAAGTACAAATCTTTGAGAAGAAGGGGTTGACTCTTGGAGTTGTTCTTCTTCTGGTTGAGTCTGGACA AGAAAAAATGTTCAAAGTTAAGGTTGTGAATGCCCTTAGATCTGCGGTGAGGAAGCCCAAGTCAACCTCTGTGAAGCTCC CATTTGGTCTTTGTGGCTGTGAAGAACAGAATGCTGGTGTTGGAGAATTCGGGGATGTTGATGTGGAGTCCATTGATCAA TGTTATAGACATGAGTTGGATGATCTGAATACTAGGATTCAGCTTCAAATGCCACCTCCAAGCTCTTCGTTTTCTGTATC AGTAGATGAATGGCAGACTATCCAATCAGGTGGTGATGATATCAGAAAATGGCTACTGAATTCAGATGATCTCGAGTTTA GTGGTCAGTTAGGACCAAATTCATTTAAAGGAGTTTACAGAGGAACAAAGGTGGCCATTGAGAAACTAAAAGGCTGTGAG AAGGGGAATTCCTATGAGTTTGCAATCCGAAAAGATTTCTTGGAGCTGATGACCTGTGGACACAAGAGCATTTTGCAGTT TTATGGTGTCTGCATCGATGAAAATCATGGATTATGCGTGGTAACAAAGTTGATGCAAGGTGGTTCACTTCGTGAACTGG TGTTGAAGAAAAAGAAGCTTCAGACCAAGTTGATATTTCAAATTGCTGTTGACATAGCAGAAGGGATGAAATTCATTAAT GATCATGGTGTTGCATATAGAGACCTTAACACACAGAGAATACTATTAGATAAGCAGTGCAACGCTTGTTTGGGTGATTT GGGAATAGTCACTGCATGCAAGAGTGTCAACGAGGCCATGGAGTATGAAACGGATGGCTATCGATGGCTTGCACCCGAGA TAATCGCGGGAGACCCGGAGAAGACAAGAGAGAGTTGGATGAGCAATGCGTATAGCTTTGGGATGGTGCTTTGGGAGATG GTGACAGGGGAGGAGGCATATGGGTCTTGCTCACCAGTGCAGGCAGCGGTTGGGATTGCAGCGTGCGGACTAAGACCAGA TATCCCAAAGGAGTGTCCTCAAGTCCTCAAATATCTTATGATCAAATGCTGGAACACTTGCCCTTCCACACGCCTTAACT TCTCTCAAATCCACTGCATCTTGCTCCGTGCCATTTCACGGTAACACATTCTCTTCTACATAGGAATTGGAAGAGTGCCT CTTTTTGAAGAAATGTTTAGCAAACCAATAGTTTTTGTGGGTAACAGAATAGCTTCACATCAA .......................N........................................................ ..............N.............................................................i... ......................................................................N......... ............i................................................................... ........N...........................................i........................... ....................................................i................N.......... ......i..............................................i.......................... .......................................i........................................ ........................................................i....................... .......N..............................i......................................... ......................................................i......N.................. .......................N...................................N...........N........ ...........................................N.........................N.......... ................................................................................ .......i.....................N.................................................. ..............................N.......................N.....N..................N ..........N........N............................i............................... .....N...N.........................N............N................N.............. ................................................................................ .............N..................................N............................... ..N..............N........N......i..................N........................... .................................................................N............N. ....i........................................................................... ...............N.....................i......N........N.........i................ ......................................i......N................N..............i.. ...................N............................................................ .....................................N.......i.................................. ................................................................................ ............N.................................................. Pos Score Pred ------------------------ 24 0.396 - 95 0.253 - 157 0.555 Yes 231 0.248 - 253 0.582 Yes 329 0.208 - 373 0.542 Yes 453 0.723 Yes 470 0.296 - 487 0.563 Yes 534 0.847 Yes 600 0.621 Yes 697 0.668 Yes 728 0.389 - 759 0.530 Yes 855 0.679 Yes 862 0.218 - 904 0.120 - 940 0.174 - 952 0.160 - 1004 0.270 - 1030 0.250 - 1128 0.816 Yes 1150 0.188 - 1231 0.230 - 1255 0.062 - 1261 0.053 - 1280 0.390 - 1291 0.301 - 1300 0.136 - 1329 0.562 Yes 1366 0.235 - 1370 0.302 - 1396 0.172 - 1409 0.449 - 1426 0.390 - 1534 0.273 - 1569 0.200 - 1603 0.274 - 1618 0.122 - 1627 0.146 - 1634 0.750 Yes 1653 0.317 - 1746 0.483 - 1759 0.456 - 1765 0.535 Yes 1856 0.295 - 1878 0.685 Yes 1885 0.178 - 1894 0.220 - 1904 0.831 Yes 1959 0.528 Yes 1966 0.273 - 1983 0.469 - 1998 0.659 Yes 2020 0.249 - 2118 0.179 - 2126 0.705 Yes 2253 0.095 -

Explain the output -- Processing locus 168: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAGGCTTTTGCGTAGGGTTTTAGATGGGCGACAAAATGATAACCAAAGCCTCTATCTCAGAAAGAGAGTCTCAATATTA TTTATTTCCCTCTTTTTATAATATGTGGACGATAATAAAAACAAAATCCTACGTGGCTTATCCATTGCGACATATAAAAC AAAAACAAAACGGTACATGAAAATTTTGCCTCAATTTTCAAAATCAATCTCAAATTCTCAAATGTCTGTGATTCGTCCTT CTCCGATTCCAATTCCGAGATGCAGATCTCAGGTACTGCATCGTCGTCTTTATTCGATTCAATTGATTCAGCGACGACGT CGACGGTGGAATCCGAGGTCGGAGGTGGAGGATACTGCGATTGAATCGACGGCTCGGTCTCCGGAGGCAGCGGGAGGGAA AATGGTGGTGGAGCTTGTTGGAGCTTTCAACGAAGTAACGGAGAGGATGAATTCGGTTTGGTTGTCGACGTCTTCGTCTA GGTTACTCTTTAAAGCTCTCAAGCTCTCGATTCCGATTTTACAGTCTCTTCCTCTTGCCTCTGATGGTCGCTCTCCTCTT TCTAAGGCTCTCTCACTCTCTATCATTCTCGCAGATCTCCAGATGGATGCAGAAGTTATCTCGGCGAGTATATTGAGTGA GGTGGTGGACGCGAATGCGATATCTATATATGAAGTTAGAGACCATATTGGTACTGGAACTGCTCATCTGCTTCATGAGA TTTTTCGTGTGAAGAATATTCCTTTTAAAGTTGATGTGTTAGACGATGAAACTGCTGCTTCATTGAGAAAGTTCTATTTA ACCTACTATGACATCAGAGCTGTGATTATGGATTTGGTTTCAAAGCTTGATGAGATGAGGCATTTGGATCACTTGCCAAG GTATCGCCAGCAGATTCTCTCTCTTGAAGTGTTGAAGATTTATTCTCCTTTAGCTCATGCGGTGGGGGCTAATCACCTGT CACTTGAGCTCGAGGACATCTCTTTCCGTTATCTTTTTCCGTGTTCGTATATATACTTAGATTCATGGTTAAGAGGTCAC GAGAACGGGTCTAAACCGTTGATAGACGTGTACAAAGAGCAGCTTCATCGGTCTTTGAAGGATGATTTGGTTTTGGCTGA AATGGTGAATGATGTATATATTAAAGGTCGGTATAAAAGTCGGTACAGCATGATGAAGAAGCTCCTAAGAGATGGGCGTA AACCGGAAGAAGTGAACGATGTGCTTGGTCTACGGGTCATATTAATGCCTAATTCTGTAGTAAACGATGTTGAAGTTGGC GAAAAAGCCTGTTACAGAACAAGTGAGATTATCCGGTCTTTGTGGAAGGAGATTCCGCATAGAACAAAAGACTACATTGC TAGGCCAAAGGAAAATGGGTATAGGAGTTTACACATGGCAGTTGATGTTAGCGACAGCGATCAGATAAGACCTTTGATGG AGATACAAATACGGACAATGGATATGGATGGATCCGCTAATGCTGGAACAGCATCACATTCTTTGTATAAAGGCGGTCTA ACCGACCCAAAAGAGGCGAAGCGGCTTAAAGCGATAATGTTGGCAGCAGCGGATCTTGCAGCGATTCGTCTCAAAGATAT CTCATCAAATAAACACCAAAGCTTCAAGACAACAACGAACCAGAGAGACAGAGTGTTTTGCCTTCTGGACAAAAACGGGG ACGGAATGATCAGCATAGAGGAACTAATGGAAGTGATGGAAGAGCTTGGAGCTCCAGGTGAAGACGCAGAGGAGATGATG CAGCTTCTTGACTCTAACAGCGATGGATCTCTTAGTTCAGACGAATTCGATACGTTTCAGAAACAAGTAGAGTTCATGCG TAAATGGGAAGATAGAGATAACGAGTACAAGAGTCTTTTAGACGAGAAGCTTCATGATCTTCCACATCAAGATACCACAG GTTTGATACAGTTATACAATAAAGAGCTTGAGGATCGTCTCTCAACCCATTAAAATATCAAACCATTTTTCTACATTCTA ATATTGTGTATATATGTATTCCTAATTAATTAATTATCTGTTGACAAAAATATGTATTTCAATCACACACAT ........................i...........N........................................... ......................i......................................................... ................N............................................N.................. ...................i............................................................ ................................................................................ ...............................................................N................ ..........................................i...N................................. ..............N..............N............................................N..... .................................N...........N.................................. .......N...................i.....................N....N......................... ........................................................N....................... ................................................................N............... .............................................................N.................. ..................N.........................N.....................N............. ................................................................................ ..............i...................i.........N...............................i... .................i.....i...N...........N........................................ ....................................N........................................... ................................................................................ .....i....................i........i......................................N..N.. ......................N....................................................N.... ...N.................................................N.......................... ................................................................................ .............N.....................................N.................... Pos Score Pred ------------------------ 25 0.515 Yes 37 0.380 - 103 0.629 Yes 177 0.183 - 222 0.449 - 260 0.754 Yes 402 0.545 Yes 447 0.365 - 544 0.250 - 603 0.906 Yes 607 0.190 - 655 0.121 - 670 0.145 - 715 0.105 - 754 0.110 - 766 0.096 - 808 0.286 - 828 0.637 Yes 850 0.111 - 855 0.409 - 937 0.274 - 1025 0.328 - 1102 0.274 - 1122 0.535 Yes 1129 0.193 - 1132 0.415 - 1170 0.309 - 1173 0.768 Yes 1192 0.316 - 1219 0.398 - 1245 0.441 - 1267 0.495 - 1375 0.593 Yes 1395 0.762 Yes 1405 0.141 - 1437 0.708 Yes 1458 0.703 Yes 1464 0.578 Yes 1468 0.480 - 1480 0.375 - 1557 0.496 - 1686 0.582 Yes 1707 0.639 Yes 1716 0.518 Yes 1755 0.222 - 1758 0.420 - 1783 0.272 - 1836 0.356 - 1844 0.287 - 1894 0.165 - 2014 0.072 - 2052 0.072 -

Explain the output -- Processing locus 169: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAGTAGTCGATAGTTCAAAACATTGACTTGAGATACACAAGTCATTTGTCAACTTGATATACACAAGTTCTAATAATTCC GACACAAGGAAATAAAGCTAGAGGAGACAGAACAGAGATAGTATGCGCATTACCAAACCCATAACTCTATATAGAGACCT TTCTTACTTCCATTCTCTACTAAAATCTTGTATCCATGGTGAAATCTCTTCTTCTCCAATCACTATCTTCCGTGACTTAC TCCGTTCCTCTCTTACACCAAATCATTTCACTATGTCTATCTTTCTCCAAGCCACTACTACCTCTTTCAATTCCTTCAAG TTACAAGTCGAACAAGTCCAAACCCATCTCACAAAATCAGGTCTTGATCGGTTCGTATACGTGAAAACATCTCTTCTTAA TCTCTACTTGAAGAAGGGTTGCGTAACGAGTGCACAAATGTTGTTCGATGAAATGCCTGAGAGAGATACAGTTGTTTGGA ATGCATTGATTTGTGGGTATTCACGAAATGGTTATGAGTGTGATGCGTGGAAGCTTTTTATCGTAATGCTTCAGCAAGGG TTTTCTCCATCTGCTACTACACTTGTGAATTTGTTACCTTTTTGTGGACAATGTGGTTTTGTATCTCAAGGGAGATCTGT TCATGGTGTTGCTGCAAAGTCTGGCCTTGAGTTGGATTCTCAGGTGAAGAATGCGTTGATTTCGTTTTACTCAAAGTGTG CAGAGTTGGGTTCAGCAGAAGTCTTGTTTAGAGAAATGAAAGATAAAAGCACAGTTTCTTGGAATACAATGATTGGTGCC TATAGCCAGAGTGGTTTACAGGAAGAAGCCATCACTGTTTTCAAGAATATGTTTGAGAAGAATGTGGAAATTAGTCCAGT CACTATCATCAATCTTCTTTCTGCTCATGTTAGTCACGAGCCACTACATTGTCTTGTTGTTAAATGTGGAATGGTGAATG ATATATCTGTGGTTACTTCTTTGGTCTGTGCATATTCAAGGTGTGGGTGTTTGGTTTCAGCAGAGCGTCTTTATGCGTCT GCTAAACAAGATAGTATTGTCGGCTTAACTTCGATTGTTTCATGCTATGCGGAGAAAGGAGATATGGATATAGCAGTTGT GTATTTTTCTAAAACGCGTCAGTTGTGTATGAAAATTGATGCTGTAGCTTTAGTTGGAATTCTCCATGGATGTAAAAAGT CTTCCCATATTGACATAGGAATGTCGTTACATGGTTATGCGATCAAAAGCGGATTGTGCACAAAAACACTGGTTGTGAAT GGACTGATAACTATGTACTCTAAGTTTGATGATGTAGAGACTGTCCTGTTTTTGTTTGAGCAACTGCAGGAGACTCCTTT AATTAGCTGGAACTCAGTGATATCCGGGTGTGTACAGTCTGGAAGGGCAAGTACAGCTTTTGAGGTGTTCCATCAGATGA TGTTAACTGGTGGACTTTTGCCGGATGCCATTACGATAGCCAGTTTGCTTGCTGGCTGTTCCCAGCTTTGTTGCTTGAAT TTGGGGAAGGAGCTTCATGGCTATACTCTTAGGAACAATTTTGAAAATGAGAACTTTGTTTGCACTGCTCTGATAGATAT GTATGCCAAATGTGGAAACGAAGTACAAGCGGAAAGTGTGTTCAAGAGTATAAAAGCTCCGTGTACAGCAACATGGAACT CAATGATATCAGGATACAGCTTATCGGGGTTACAACATCGAGCTCTTTCTTGTTACTTGGAAATGCGAGAGAAGGGGTTG AAACCTGACGAGATCACCTTTTTGGGGGTTCTGTCTGCATGTAACCATGGAGGCTTTGTCGATGAAGGAAAAATATGTTT CAGAGCAATGATCAAAGAGTTTGGAATATCTCCAACTTTACAGCATTATGCACTTATGGTTGGCTTGTTGGGAAGAGCAT GTTTATTTACTGAGGCATTGTATTTAATTTGGAAGATGGATATAAAGCCGGATTCTGCAGTATGGGGTGCTCTACTTAGC GCGTGCATTATTCACAGAGAGTTGGAGGTTGGGGAATATGTAGCTAGGAAAATGTTTATGCTGGATTACAAGAATGGAGG GCTGTATGTATTGATGTCAAACCTATATGCAACAGAAGCAATGTGGGACGACGTAGTTAGAGTCAGGAATATGATGAAAG ATAATGGATACGATGGTTATTTGGGAGTTAGCCAAATATAATAATTTGAATCTGTGAAACAACAGAACAGGTTACTTATA CGTTCTGTTAATACCTCAGTATTTTTACCTGATTGCTTTGCGATGGTGAGGAGAGTTAAC ................................................................................ ..........................................i..................................... ...................................N............................................ ................................i............................................... ................................................................................ .....................................N.........N....i........................... N..........................N.....N........N......................N.............. ..................................................N............................. ..N...............................................N............................. ...................................i................................i........... ................................................i............N.................. ..........................N....................................N......i......N.. ........................................................................N....... .........................................N....N................i................ ............................N.........N..........................N...N.......... ....................i.........N.....N.........................................N. ............N...............N..N................................................ ............................................................................N..i ........................N....................................................... ................N.............................N...............................N. ..N......N..............................................................i....... ..N...........................................................i................. ......................................N.......N..............N............N..... .......N.......................................N.......i......................N. ...................................i.........................i.................. .....................................N.............i.....N...............N...... .....N.......i............N.............i.............................N..N...... ...N........N................................................................... ..........................................N................. Pos Score Pred ------------------------ 123 0.519 Yes 196 0.451 - 273 0.625 Yes 438 0.449 - 448 0.229 - 453 0.730 Yes 481 0.199 - 508 0.206 - 514 0.301 - 523 0.123 - 546 0.241 - 611 0.230 - 643 0.218 - 691 0.124 - 756 0.539 Yes 789 0.680 Yes 849 0.598 Yes 862 0.120 - 907 0.067 - 944 0.257 - 951 0.713 Yes 958 0.179 - 1033 0.079 - 1082 0.119 - 1087 0.086 - 1104 0.739 Yes 1149 0.383 - 1159 0.131 - 1186 0.203 - 1190 0.402 - 1221 0.688 Yes 1231 0.091 - 1237 0.060 - 1279 0.204 - 1293 0.420 - 1309 0.265 - 1312 0.296 - 1437 0.304 - 1440 0.635 Yes 1465 0.153 - 1537 0.261 - 1567 0.449 - 1599 0.499 - 1603 0.362 - 1610 0.500 - 1673 0.684 Yes 1683 0.210 - 1743 0.628 Yes 1799 0.130 - 1807 0.341 - 1822 0.248 - 1835 0.418 - 1848 0.494 - 1888 0.097 - 1896 0.509 Yes 1919 0.491 - 1956 0.558 Yes 1982 0.597 Yes 2038 0.219 - 2052 0.524 Yes 2058 0.392 - 2074 0.298 - 2086 0.118 - 2094 0.669 Yes 2107 0.246 - 2121 0.662 Yes 2151 0.278 - 2154 0.300 - 2164 0.289 - 2173 0.106 - 2283 0.056 -

Explain the output -- Processing locus 170: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTAGCACAAAAATTTCAAAGTTGTCAATTTCTTATTAATGTGCATGGTTATTCAAATGGAAATTTTAAACTGATGATTAA ATTTCTAAGAACCACAAATAGTCAAATACTAACTACACCACCCACACATTATCTCAAGAAAAAAGAAAAATGGCAACGAA GAGTTGTGAATTAGTCCTCTGTTTCTTCGTCTTTTTCGTCATAAGCTTCAGTGCAATTTCTGTTTCTGCACAAACTTGTG ACAATACTACTGGGACTTTCATACCCAATAGTCCTTATGATAAGAACCGCCGACTCATCCTCTCTACTCTAGCTTCTAAC GTCACGGCTCAGGAGGGCTACTTCATCGGTTCGATTGGGATTGCTCCTGACCAAGTCTTTGCCACCGGGATGTGCGCTCC AGGTTCTGAGCGTGATGTTTGCTCTCTCTGTATCAGGTCCACATCAGAGAGTTTACTACAAAGCTGTCTGGACCAGGCAG ATGCTTTCTTCTGGTCAGGTGAAGAAACCCTTTGCCTTGTTCGCTACGCTAACCGCCCCTTCTCTGGATTGCTCGTCATG GATCCACTAGGAGCGATATTCAACACAGGAGAACTCAATACGAATCAGACTGTGTTTGATATCGAGTGGAATAATTTAAC TTCTAGTATGATCGCCGGAATAACTTCTTCTTCATCAGGGGGGAACAATTCGTCTAAGTACTACTCAGACGATATAGCTC TTGTGCCGGATTTTAAGAACATATCAGCGTTGATGCAATGCACACCAGACGTTTCGTCAGAGGATTGTAACACTTGCTTA CGGCAAAACGTTGTCGACTATGATAATTGTTGCCGTGGGCACCAAGGCGGCGTTATGTCAAGGCCAAACTGCTTTTTCCG ATGGGAAGTGTATCCCTTCTCTGGTGCTATTGATCAGATTAATTTACCAAAGTCACCGCCGCCTTCAGTTACATCCCCGT CTCCTATAGCCAACATAACCAAGAATGGAAATAGAATATCAGGAGGAAAAATCGCAGCAATAGTTGTTGTTACTGTTGTT ACAATAATATTGGTTGTTTTAGGCTTTGTTATTTCCAATAGAAGAAAGCAAAAGCAAGAAATGGATCTTCCAACGGAATC TGTTCAATTTGATTTAAAGACAATTGAATCGGCGACAAGCAACTTTTCCGAACGTAACAAGCTTGGTAAAGGTGGATTCG GTGAAGTTTACAAGGGTATGCTCATGAATGGCACTGAAATTGCGGTGAAGAGATTGTCGAAAACATCAGGACAAGGTGAA GTAGAGTTCAAGAACGAGGTTGTTGTTGTTGCAAAGCTTCAACACATAAATCTTGTTAGACTTCTCGGGTTTTCGCTACA AGGAGAAGAGAAGTTACTCGTCTATGAGTTTGTTTCAAACAAAAGCCTCGATTATTTCCTCTTTGATCCTACGAAGAGAA ATCAGTTGGACTGGACAATGCGACGCAATATCATCGGTGGCATTACTCGAGGGATTTTATATCTTCATCAAGATTCGCGG CTGAAGATCATACATCGTGACCTCAAAGCGAGTAACATTCTCTTAGATGCGGATATGAATCCAAAAATCGCTGATTTCGG AATGGCAAGGATCTTTGGAGTGGACCAAACTGTTGCCAATACAGGAAGAGTGGTTGGAACCTTTGGTTACATGTCTCCTG AGTATGTGACACATGGCCAATTCTCAATGAAATCAGACGTCTATAGTTTTGGAGTATTGATTCTTGAGATCATTAGTGGC AAAAAGAATAGCAGCTTCTACCAAATGGATGGCTTAGTTAACAATTTAGTCACATATGTCTGGAAACTTTGGGAGAACAA ATCATTGCATGAGCTCTTAGATCCCTTTATCAACCAAGATTTCACAAGCGAGGAAGTCATTAGATACATTCATATTGGAC TGTTATGTGTTCAAGAAAATCCTGCAGATCGACCAACAATGTCTACGATTCACCAAATGCTCACAAACAGCTCAATCACT CTGCCTGTGCCTCTGCCTCCTGGATTTTTCTTTAGGAACGGACCAGGATCGAACCCGGGTCAGTCGAACAGCAAGTCTTT TGCTTGTTCTGTAGATGAAGCAACAATCACGGACGTTAATCCTCGTTGAAAATTCAGCATTTGGTTTTTTCTTGTTTCCA CAAACGTCTATCATTTATTTATTGTCCGTTTATGAAAAAGTTTACAAAGGGTCATTTTTTCAGGTTGA .....................................N.....N...........N................N....... .....................................................................i.......... ................................................................................ ....................................N........................................... .....................................................................N.......... ..............N................................................................. i............................................................................i.. ................................................................................ .......i........................................................................ ................................N....N.......................................... ...................N..................................i......................... N............................................................................... ........................N....................................................... ............................................................i................... ................................................................................ .................i.....N...N.................................................... ................................................................................ .......................i........................................................ .................i.............................................................. ..............................................N.......i......................... ...N........N.............N..................................................... ........................N...N..........................N........................ ........N....................................................................... ....i.................................i.................i....................... ................................................................................ ..............i................................................................. ...............................N.................................... Pos Score Pred ------------------------ 38 0.214 - 44 0.306 - 56 0.492 - 73 0.211 - 150 0.885 Yes 277 0.246 - 390 0.314 - 415 0.272 - 481 0.502 Yes 558 0.751 Yes 648 0.739 Yes 753 0.460 - 758 0.110 - 820 0.258 - 855 0.750 Yes 881 0.434 - 985 0.361 - 1101 0.736 Yes 1218 0.558 Yes 1224 0.424 - 1228 0.143 - 1384 0.526 Yes 1458 0.697 Yes 1567 0.182 - 1575 0.732 Yes 1602 0.833 Yes 1671 0.390 - 1684 0.226 - 1693 0.396 - 1707 0.259 - 1785 0.452 - 1789 0.402 - 1816 0.147 - 1849 0.187 - 1925 0.675 Yes 1959 0.745 Yes 1977 0.533 Yes 2095 0.539 Yes 2192 0.033 -

Explain the output -- Processing locus 171: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGAGAAAGTGTTGAGAGAGAGAACGTGGTCTGTTTGGGTACAGGTATTAAACTATTCTTAGTTAGAATTAGACTATCCGG GGTTAAACTCTATTCATATAACCAAACGATATGGTTAAATTCGCGATTATTAATACTCTCACTGTGAACGAAACTTGGGC GAAATTGAAAAGTTTTGGCGTTATGGAATCGTCCATTGAAGGGAGCTCCGAGTCAACTACTGTCACTACTTCTCCTTCGC GTCGTGTACGAGAACTTTTAGCTTTGTGTTTCAGTTCAGTTGAAGAAGCCGGAGGATTTCAAGACTTTGAGAGTTTTGTG ACAGAACTTGTCAGCTGTTTGGATTCTTTGTATGAGAACGTAGCTTTGGATGCTAACAATGAACTGGAGAACGATGTTAT TGAAGAAGTATTAGATGAAATTTTGAAAGTTTTATCATCTCCTCAAATGGATCAGGATGTTATAGATGCACTGTCATTTC ACTTGCCAAAAGTAACTTCCAAGTTTGCAGACATATCAAGCAGATGTTTACAGTTAGTTGAGGAAATCGTTGATCGGTTT GTGGAAGCATGCAATCCACGGGATATGCTTTCAATTCTTTGTGAGGCACTCGATGCTGCACGGTGCTATCATTCAGCCTC CACTTGTTCTACTCCTTTATTGCATGGGCTCTCTAAAGTATTTATTTTGATCCAGAGGCGTCACTACGAGCAATTAAAGG TTGCAGTTCCAATTGTCCTGAACGTTTTGAAGGACATTTCATTGGAAACAGACGTGCAAGTTGAAGATTTATTTGATAAG GCTCTAGGCATTGCCTCTTCAATTCGAGACGTTTCTTCAAAACTGAATAACGAAGAAGAAGCAAAAGTCCGTTGTCTACT TTGTCTCTATGTGATACAGATAACGGCTATTATTTCAGTTAGCATCAGAGACAAAGCGGCCTCTTGTATTCCTCTGGTGA TTCAGTTAGAACCGTTCTTGACATCCTGTGGTTTAACACATCTTGGTTTAATCACGGGGAATGACACTGAAAAATTAATG AGCACGGTTGCTGGAGATGATGATGAGTTTATCACCTCTTTCCCTGACATCAGCTTGGGTGCATCGCTTTTATTCATCTG TGCAAAGATCTCCCATGAGGTTGCAGAGGCGGCTAATGCGGTTTTAGGAAGTGTTGTTGATGAGCTTCAAAACAATCCAG TGAAAAGGTGGCAAGCTTATGGGATGTTGAAATATATACTTTCCTCTGGAGATCTGCTCTGGGAATTCAAAAGACATGCT ATCGAGTTCTTGCTTGACATAACAAAAGGAGTTACTTCATCTCAATGCAATGATGAGCAAATAGATTGTTCGGACTACAC GCCTGGCATCTACGCTACTTTACAGGTTCTTTCTGATATCCCAGCTCCTCATAGGTTTGACGTTTTAAGAGCCCTTGTCA CAAATTCCCGGTCTCCTTCAATGACAGCAATCCTTCTGGGTCTAGTTAAAGACAGCATGAGCAAAAGCAGTTTGCAAGAT ACAGATTGTGCGGCTGTTGATACGCATGTTATTGAGCTAGTGGAGTTGGTCTTAAGGCCTCCACAGGGAGGTCCTCCACT CCTTCCGGACCAGAGTGATGCGATCCTGGCGGCACTTAACCTCTACAGGTTTGCACTGTTATTTGAATCGAGAGAATGTG AAGCAGGTAAAGAGAGAAGCAAAGTCGGCAGTGATATTTTGTCAAAGAAGAATCTGGAGAAAGCGTATAAGGAATGGCTT CTGCCTCTTCGAACTCTTGTGAGCTGCAGCATCGCTGAGAACCTCAAGGAGGATCATGGCCAAGAATCTTCACTCGACGA TGTAGGGCTTCTGAACCCAATTGAATTGGTTCTATATCGATGCATCGAGCTCGTGGAAGAAAAGTTGAAAAGTCATTAGT CAAAAAAATAGTGAGAACTTCAATTGTAGGCTCATTGTTAGTTACAAAAAAAATTTGTTTGATATTTTCCTGCAACTTTG GTTATTCCAACTTGAGTC ................................................................................ ..............................i................................................. ......................N......................................................... ................................................................................ ...............................N.................N........N..............N...... ..............N...............................N.........N........N.............. ...........................................N.................................... ........N...............N...........................N........................... .......................N........................................................ ................................................................................ ................................................................................ ........N....................................................................... ............................................................N................i.. ................N..N..N......................................................... ..............i....................N.......................N.................... ..................N....N...................................................N.... ............................................N....N..N........................... ................................................................................ ....................i...................................i....................... .........................N...................................................... .................N.........................................................i.... .........................................................................i...... .......................................................N.......................N .......................................N........................................ ................................................................................ .................. Pos Score Pred ------------------------ 111 0.686 Yes 183 0.229 - 352 0.108 - 370 0.057 - 379 0.182 - 394 0.092 - 415 0.106 - 447 0.452 - 457 0.277 - 466 0.176 - 524 0.336 - 569 0.315 - 585 0.154 - 613 0.296 - 664 0.187 - 889 0.116 - 1021 0.491 - 1038 0.611 Yes 1057 0.307 - 1060 0.247 - 1063 0.179 - 1135 0.530 Yes 1156 0.158 - 1180 0.167 - 1219 0.429 - 1224 0.232 - 1276 0.336 - 1325 0.122 - 1330 0.369 - 1333 0.343 - 1461 0.641 Yes 1497 0.627 Yes 1546 0.159 - 1618 0.356 - 1676 0.503 Yes 1754 0.614 Yes 1816 0.245 - 1840 0.291 - 1880 0.173 -

Explain the output -- Processing locus 172: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGATGTGAATTACTAACCTACCATAAAAAAGATGAGAAATAGAAGTTAGAAATACTTTTCTAGTAACTTTCACACTATAT AATATGCAAGAAATTAATTAAAAACGTCACAAAGATATCTTAATTTGCAGCAAGAGACAAAAAGTAATGGCAATCCAGCT ATATAGTGAACACAAGTATATGACTGTACCGCCTAAAACCTTTCCTTTAAATTCCCCTTTCTACCTCTTTTTTTTTTTCC CTCAGAGTAGTACTCTAAACCTCAAGTTTACCTCTACTTCTCTTATACCATTCCGCCTCTTATTCTTTGCAATTTCTCTC AACAAAGTAGAAATGGGATTGACCTCATCCTTACGGTTCCATAGACAAAACAACAAGACTTTCCTCGGAATCTTCATGAT CTTAGTTCTAAGCTGTATACCAGGTAGAACCAATCTTTGTTCCAATCATTCTGTTAGTACCCCAAAAGAATTACCTTCTT CAAATCCTTCAGATATTCGTTCCTCATTAGTTTCACTAGATTTGGAGGGTTATATAAGCTTCGACGATGTCCACAATGTG GCCAAGGACTTTGGCAACAGATACCAGTTACCACCTTTGGCAATTCTACATCCAAGGTCAGTTTTTGATATTTCATCGAT GATGAAGCATATAGTACATCTGGGCTCCACCTCAAATCTTACAGTAGCAGCTAGAGGCCATGGTCACTCGCTTCAAGGAC AAGCTCTAGCTCATCAAGGTGTTGTCATCAAAATGGAGTCACTTCGAAGTCCTGATATCAGGATTTATAAGGGGAAGCAA CCATATGTTGATGTCTCAGGTGGTGAAATATGGATAAACATTCTACGCGAGACTCTAAAATACGGTCTTTCACCAAAGTC CTGGACAGACTACCTTCATTTGACCGTTGGAGGTACACTATCTAATGCTGGAATCAGCGGTCAAGCATTCAAGCATGGAC CCCAAATCAACAACGTCTACCAGCTAGAGATTGTTACAGGGAAAGGAGAAGTCGTAACCTGTTCTGAGAAGCGGAATTCT GAACTTTTCTTCAGTGTTCTTGGCGGGCTTGGACAGTTTGGCATAATCACCCGGGCACGGATCTCTCTTGAACCAGCACC GCATATGGTTAAATGGATCAGGGTACTCTACTCTGACTTTTCTGCATTTTCAAGGGACCAAGAATATCTGATTTCGAAGG AGAAAACTTTTGATTACGTTGAAGGATTTGTGATAATCAATAGAACAGACCTTCTCAATAATTGGCGATCGTCATTCAGT CCCAACGATTCCACACAGGCAAGCAGATTCAAGTCAGATGGGAAAACTCTTTATTGCCTAGAAGTGGTCAAATATTTCAA CCCAGAAGAAGCTAGCTCTATGGATCAGGAAACTGGCAAGTTACTTTCAGAGTTAAATTATATTCCATCCACTTTGTTTT CATCTGAAGTGCCATATATCGAGTTTCTGGATCGCGTGCATATCGCAGAGAGAAAACTAAGAGCAAAGGGTTTATGGGAG GTTCCACATCCCTGGCTGAATCTCCTGATTCCTAAGAGCAGCATATACCAATTTGCTACAGAAGTTTTCAACAACATTCT CACAAGCAACAACAACGGTCCTATCCTTATTTATCCAGTCAATCAATCCAAGTGGAAGAAACATACATCTTTGATAACTC CAAATGAAGATATATTCTATCTCGTAGCCTTTCTCCCCTCTGCAGTGCCAAATTCCTCAGGGAAAAACGATCTAGAGTAC CTTTTGAAACAAAACCAAAGAGTTATGAACTTCTGCGCAGCAGCAAACCTCAACGTGAAGCAGTATTTGCCCCATTATGA AACTCAAAAAGAGTGGAAATCACACTTTGGCAAAAGATGGGAAACATTTGCACAGAGGAAACAAGCCTACGACCCTCTAG CGATTCTAGCACCTGGCCAAAGAATATTCCAAAAGACAACAGGAAAATTATCTCCCATCCAACTCGCAAAGTCAAAGGCA ACAGGAAGTCCTCAAAGGTACCATTACGCATCAATACTGCCGAAACCTAGAACTGTATAAAAGTTTCCTGTGTCCGTCCT TGTAACCGCTCAGGCTAGGCAGCAAGAAAGTGAAAAATTCTTTCTTTTTTGTTTCTTTTGGTGAACAACAAATTTTACA ..N............................N................................................ ...N..............................................................i............. ...................N............................................................ ................................................................................ ............i..............................................................N.... ................................................................................ ..................................................................N........N.... ..............................................................................i. ................................i............................................... ....N.....N..................i.................................................. ............................................N.............................i..... ................................................................................ ................................................................................ ....N.......N................................................................... ................................................................................ .....................................N.......................................... ...................i............................................................ .........................................................................N...... ................................................................................ ................................................................................ ...N............................................................................ ........................N...................................................N... ....................................i........................................... ................................................................................ ................................................................................ ............................................................................... Pos Score Pred ------------------------ 3 0.196 - 32 0.452 - 84 0.203 - 147 0.593 Yes 180 0.408 - 333 0.733 Yes 396 0.155 - 547 0.290 - 556 0.352 - 639 0.560 Yes 642 0.449 - 700 0.294 - 753 0.718 Yes 805 0.442 - 811 0.248 - 830 0.536 Yes 925 0.486 - 955 0.591 Yes 1125 0.459 - 1133 0.356 - 1318 0.180 - 1380 0.523 Yes 1514 0.343 - 1684 0.452 - 1785 0.374 - 1837 0.215 - 1877 0.500 -

Explain the output -- Processing locus 173: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAATATAGCTTCCATTCCTCTAAAAGGTCCTTTAATATCATGTTTCTGTTATAAACTCAAAAAGAAAGTGAGATTGTGA CTTGTGTACTTAGAGCTTTAGATTGTTTCTATGACAGTTTCCAAAAGTTTAGGCACTGCCAGCATGCAATCCATTGGTTA TGCTACAAATATTAAATTTTGTTATCTATCACTCTCAGTCATATGTTTTAAGGCCTCTCACAATTTGCTATATATAATAT GGATTTCCAAATCTTTATTGCCACACATCCACTCATACAAGATGGATAATCTAGTGTTATGTGAAGCAAACAATGTTCCT CTAACGCCCATAACGTTCTTGAAGAGAGCTTCGGAGTGTTATCCCAACCGAACTTCGATAATCTACGGGCAAACTCGCTT CACTTGGCCTCAGACTTATGACCGTTGCTGTCGTCTAGCTGCTTCTCTCCTCTCTCTTAACATCACCCGAAACGACGTTG TCTCGATTTTGGCCCCAAACGTACCTGCCATGTACGAGATGCACTTTAGCGTTCCCATGACCGGAGCCGTACTTAACCCC ATTAACACCCGTCTTGACGCCAAAACCATAGCCATCATTCTCCGCCACGCCGAGCCAAAGATCTTATTTGTGGACTACGA GTTTGCTCCTTTGATCCAAGAAGTCCTCCGTCTCATACCCACTTACCAGTCACAACCGCATCCGCGGATCATATTAATTA ACGAGATTGATTCCACTACAAAGCCTTTCTCCAAGGAGTTAGACTACGAGGGTCTCATCCGAAAAGGAGAACCTACGCCA TCCTCCTCCGCAAGCATGTTCCGTGTCCACAACGAGCATGACCCTATCTCCTTGAACTACACGTCAGGTACCACGGCAGA CCCAAAAGGTGTGGTGATTAGCCACCAAGGAGCCTACTTGAGCGCTTTGAGCTCCATTATTGGCTGGGAAATGGGGATTT TCCCTGTCTACCTCTGGACACTACCTATGTTTCATTGCAATGGATGGACGCACACGTGGTCCGTCGCTGCTCGTGGTGGT ACTAATGTATGCATCAGACATGTCACTGCTCCAGAGATCTATAAGAACATAGAATTGCATGGCGTGACTCACATGTCATG TGTTCCTACGGTCTTTAGGTTTCTCCTTGAAGGAAGTCGAACCGACCAGTCACCCAAGTCCTCGCCGGTTCAGGTGTTGA CTGGAGGTTCTTCTCCTCCGGCTGTCCTTATAAAGAAAGTTGAGCAGCTGGGGTTTCACGTGATGCATGGCTATGGGCTT ACAGAGGCCACCGGCCCGGTTCTCTTTTGTGAATGGCAAGACGAGTGGAACAAACTACCAGAGCATCAACAGATAGAGCT ACAACAAAGGCAAGGTGTAAGGAACCTAACCCTAGCCGACGTTGACGTGAAGAACACGAAAACTCTAGAGAGTGTCCCAC GCGACGGGAAGACAATGGGAGAAATTGTTATTAAAGGAAGCAGCCTAATGAAAGGGTATCTAAAGAATCCTAAAGCAACA TCTGAAGCATTTAAACACGGATGGCTTAACACGGGAGACATAGGTGTGATTCATCCTGATGGATATGTAGAGATCAAAGA TCGATCCAAAGACATCATAATCTCGGGAGGTGAGAACATAAGTAGTATTGAGGTCGAGAAAGTTCTTTACATGTATCAGG AAGTGCTCGAGGCTGCGGTTGTAGCCATGCCTCATCCTCTATGGGGTGAAACCCCGTGTGCGTTTGTTGTTCTAAAGAAG GGTGAAGAGGGATTGGTGACTAGTGAAGGAGATCTCATTAAATATTGCCGTGAGAATATGCCGCATTTTATGTGTCCGAA AAAGGTGGTGTTCTTTCAAGAGCTTCCAAAGAATAGTAACGGAAAGATCCTAAAGTCTAAGTTGAGAGACATCGCTAAGG CATTGGTGGTCAGGGAGGACGATGCCGGCTCCAAGAAAGTTCATCAACGCAGTATCGAACACGTTAGTTCGAGGCTTTAG CGTTACTATTCATCGATGTGTTTATGTATATGCGTATGTTTGGATTGATATGTTTCTTAGTTTACATGTATGGATTGTTA AATTTACGCTAATAATTAA ........................................N....................................... ..............................N................................N...............N ..........................................N...................................i. .........................................i................i.............N....... ................................................................................ .................N.............................................................. .............................i........i.................i....................... ................................................................................ ................................................................................ ................................................................................ ...............i.....................N.......................................... ......................................................................N......... ..........................i............i...N.................................... ....N...N..........N......................................N.............i....i.. ................................................................................ ..............................................................i...i.....i....... ................................N............................................... ................................................................................ ..............i................................i................................ ....................N.....................................N.....N............... ......................................................................i......... ..........................N.............i....................................... .........................................................i...........N.......... ................................................................................ .....................i.......................................................... ...............N.......N.....N.....N.............N...............N...N.......... ................... Pos Score Pred ------------------------ 41 0.311 - 111 0.466 - 144 0.129 - 160 0.125 - 203 0.348 - 239 0.739 Yes 282 0.575 Yes 299 0.662 Yes 313 0.431 - 418 0.366 - 510 0.836 Yes 519 0.800 Yes 537 0.575 Yes 816 0.557 Yes 838 0.330 - 951 0.492 - 987 0.552 Yes 1000 0.780 Yes 1004 0.390 - 1045 0.114 - 1049 0.199 - 1060 0.315 - 1099 0.427 - 1113 0.538 Yes 1118 0.823 Yes 1263 0.685 Yes 1267 0.679 Yes 1273 0.598 Yes 1313 0.277 - 1455 0.541 Yes 1488 0.543 Yes 1541 0.431 - 1579 0.300 - 1585 0.101 - 1671 0.746 Yes 1707 0.410 - 1721 0.511 Yes 1818 0.709 Yes 1830 0.482 - 1942 0.628 Yes 2016 0.071 - 2024 0.045 - 2030 0.038 - 2036 0.062 - 2050 0.048 - 2066 0.033 - 2070 0.061 -

Explain the output -- Processing locus 174: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCGAGTGAGTCCCCACGTGCAAACTAAAAAATCCCAAAAAAAAGAGTAAAAAAATGTCTGAGCCCGGGTTCGAACCGGGG ACCTCTAGTGTGTGAGACTAGCGTGATAACCGACTACACCACCCAGACTTTTTATTAATTCAATTAAGTTTGTATTTAAT AAGGAAGCTTAACAAAATTGTGATGCGCTCTGTTTTTTTTTTTTTTTTTTTTCGTCTTTGTGATGCGCCGCTTTCGTCAA CAAATTGCCGAAAAAGAGTAAACAAAAAGCAAAAGAAGTGTAGCAATGTTAGTGACAAGAGAATGTTTGCAGTTTCGATA GTACCTCGAACCACATCGTGCCGTTTGAGCTCTGCCTTTCTCTGTCAACTCTCGATTCCTCTCACTCTTCGGCTCCACCA TCACTACCAACACCACCAGCCTCACCTACCATCTCCTCTCTCTTTTCAGATTCATTCGTTAAGAAAGCAGATCGACATGG CAGCTCAAGGAGGTGATTCATATGAGGAAGCGTTGGCTGCTTTGTCGTCTTTGATCACGAAACGAAGTCGTGCTGATAAG AGCAATAAAGGGGATCGCTTTGAGTTAGTCTTTGATTATCTCAAGCTACTTGACCTGGAAGAAGACATTTTAAAGATGAA TGTTATTCATGTCGCTGGTACCAAAGGCAAGGGATCCACATGTACCTTTACAGAGTCTATTATTCGAAACTATGGCTTTC GAACTGGACTCTTCACTTCACCTCACCTCATTGATGTCCGGGAAAGATTTCGTTTGGATGGTGTGGACATAAGTGAAGAG AAATTTTTGGGATATTTCTGGTGGTGCTATAACAGGCTCAAGGAGAGAACTAACGAGGAGATACCAATGCCTACATATTT CCGCTTCCTTGCATTGCTAGCTTTTAAAATATTTGCTGCAGAAGAGGTAGATGCTGCTATATTGGAGGTTGGATTAGGTG GAAAGTTTGATGCCACCAATGCGGTTCAGAAACCTGTGGTATGTGGTATTTCTTCACTCGGATATGACCACATGGAAATT CTAGGTGATACACTTGGAAAAATTGCTGGTGAGAAGGCTGGAATTTTCAAGCTTGGAGTTCCAGCTTTCACAGTGCCCCA ACCTGATGAAGCCATGCGTGTCCTTGAAGAGAAAGCTTCCGAAACAGAAGTGAATCTCGAAGTGGTGCAGCCACTAACCG CAAGGCTGTTAAGTGGTCAGAAACTTGGGCTTGATGGGGAACACCAATATGTCAATGCTGGTCTAGCAGTTTCGCTTGCC TCTATCTGGCTTCAGCAAATTGGTAAACTAGAAGTTCCGAGTCGGACTCAGATGAGTATTCTGCCTGAGAAATTCATCAA AGGGTTAGCTACAGCGAGTTTGCAAGGACGAGCACAGGTCGTCCCTGATCAATATACTGAATCTCGGACTTCAGGAGATC TAGTATTTTATCTGGATGGAGCTCACAGTCCAGAAAGCATGGAAGCATGCGCCAAATGGTTTTCGGTTGCGGTTAAGGGA GACAACCAGTCAGGGAGTTCAGGACATTTGGTTAATGGCTCTGCAGGATCCTCTCATGATAAATGGTCAAATGAAACCTG TGAACAGATATTGTTGTTCAATTGTATGTCAGTTCGGGACCCAAATCTACTGCTTCCACATCTAAAGAATATGTGCGCAA AATACGGTGTCAATTTCAAGAAGGCATTGTTTGTACCAAACATGTCGGTGTATCATAAGGTTGGTACAGCAGCTGATTTG CCAGAGAATGATCCACAGGTTGACTTGTCATGGCAGTTCACACTTCAGAAAGTGTGGGAAAGCCTTGTGCAGAGTGAAAG AGATGGAGAAAAAGATGGTGAAAGTGATGGAAACAGTGAGGTGTTTACTTCACTACCCATGGCAATAAAATGTCTAAGGG ACACTGTACATGAGAGTAGCTCAGCCACACGTTTCCAGGTCCTTGTAACTGGTTCGTTACATCTTGTGGGCGATGTACTG AGATTAATCAGAAAATGAAGAGAAACAAACACACTTCACTTTTCAAATTTCAATACACGAGTCGTCATCCACCAATCACC GTACCCACTGTGGAAGAGGATGGCATTTCTTATTAAA .....................................................i.......................... ................................................................................ ......................N.......................................N................. .............................................i................N................. ................................................................................ ............................................................................i... .....................N.......................................................... ...........................................................................i...N ........N..............................N...............................N........ .................................N.......................N...................... ..................................................................i............. ..................................................N............................. .........N........N.....................N......................N.......i........ ................................................................................ .....N.......N.................................................................. .................................N..............N.....N......................... ...................................................N............................ ................................................................................ ...............N......................i.......N........N........................ ..................................N....................N......i.......N......... .........................N............................................N......... .........................................i...................................... .......N.....................N.................................................. ..N...........N...........N...............................i..........i.......... .........N..............................................................N....... ..............i................................................................. ...................N................. Pos Score Pred ------------------------ 54 0.670 Yes 183 0.410 - 223 0.182 - 286 0.829 Yes 303 0.371 - 477 0.843 Yes 502 0.250 - 636 0.579 Yes 640 0.195 - 649 0.457 - 680 0.336 - 712 0.494 - 754 0.495 - 778 0.360 - 867 0.709 Yes 931 0.274 - 970 0.115 - 979 0.264 - 1001 0.282 - 1024 0.173 - 1032 0.869 Yes 1126 0.110 - 1134 0.442 - 1234 0.161 - 1249 0.356 - 1255 0.165 - 1332 0.487 - 1456 0.279 - 1479 0.825 Yes 1487 0.240 - 1496 0.232 - 1555 0.158 - 1576 0.159 - 1583 0.506 Yes 1591 0.161 - 1626 0.251 - 1671 0.487 - 1722 0.756 Yes 1768 0.275 - 1790 0.202 - 1843 0.330 - 1855 0.423 - 1867 0.296 - 1899 0.588 Yes 1910 0.708 Yes 1930 0.477 - 1993 0.238 - 2015 0.629 Yes 2100 0.052 -

Explain the output -- Processing locus 175: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATTCAATAGAAGAATTTTGGTATAGTTCGTACAGATGGAAGTTGAAATTAGTTGAATTGCCGTTTTCTATACGTACGA ATTTTGGTATTTATTGATTAGATAAACCGGAACAAAACCGGTTTAGTAAACCGACGCATGGTGTTGGTAAAGTGTGATTG GGTAAGAAGCCTAACTAGTTGTCTGTTGATAATTGTCACTAGGTGGGCACTTAAAGAAAAAAACACTTGTTTCCTTATAT ATTCTTCTCCCTTTAAACATTTAATCTTTTCCTCTTCTACCATCTCCACAAATTCCAAACATCTCTCTCTCTTTCTCTCT CACACACAAAATTGCAGAAGAAGAAGAGTCATGAATGGTGAAGAAAGCTTTGTAGAAGATTGCTCTGTTTTTGTTGAGAT TGATCCTTCTGGAAGATATGGAAGATACGATGAAATACTTGGCAAAGGAGCTTCAAAGACAGTATACAGAGCATTTGATG AGTATGAAGGTATAGAAGTAGCATGGAACCAAGTAAAGCTTCGAAATTTCACAAGGAATCCTGAGGAATTAGAGAAGTTT TTCAGAGAGATTCATCTTCTCAAGACTTTGAATCATCAAAACATTATGAAATTCTACACTTCTTGGGTTGATACCAACAA TTTATCAATCAATTTTGTCACTGAACTCTTCACCTCTGGTACTCTCAGACAGTATAGGTTGAGACATAGAAGAGTGAATA TTAGAGCAGTGAAGCAATGGTGCAAGCAGATTTTAAAAGGGCTTCTTTATTTACATAGTCGTTCTCCACCAATTATACAT AGAGATCTCAAATGTGATAACATTTTCATCAATGGAAACCAAGGTGAAGTCAAGATCGGTGACCTTGGACTCGCTGCGAT TCTTCGTAAATCACATGCCGTTCGTTGCGTTGGAACCCCTGAGTTTATGGCTCCAGAAGTGTATGATGAGGAATATAATG AGTTGGTTGATGTATATGCTTTTGGCATGTGTGTGTTGGAGATGGTTACTTTTGATTATCCTTACAGTGAATGTACTCAC CCGGCACAAATCTACAAGAAAGTTACCTCGGGGAAAAAGCCTGAAGCTTTTTACTTAGTGAAGGATCCTGAGGTTCGTGA GTTTGTTGAGAAGTGTTTAGCTAACGTGACGTGTAGGCTAACGGCATTGGAGCTTTTACAAGACCCTTTTCTACAAGATG ATAATATGGATGGATTTGTTATGAGACCTATTGATTACTACAATGGTTATGATGAAACTGGTGTGTTCCTTAGACATCCT TTGATTGATGATCCTCTTTACCATGATCAGTTTGAGTCGTCACAGATATGTGAGATCGATCTTTTCGCTAACGATGATGA AGATCATGTCGACATTTCGATTAAAGGGAAGAGAAACGGTGATGATGGGATATTCTTGAGACTTAGAATATCTGATGCTG AAGGACGGATAAGGAACATTTACTTCCCGTTTGAGACGGCTATTGATACTGCATGGAGTGTAGCGGTTGAGATGGTGTCA GAGCTCGACATAACGAATCAAGATGTTGCGAAAATCGCGGAGATGATCGATGCAGAGATTGCTGCATTGGTGCCTGATTG GAAAAATGATACAGAAAGTTCCCAAAATGTAAACAACAACAAGAACAACAACACTGCAGGATTCTGTGGAGAGTGTGCTT CAAACGGGTATATACAAGAGACTGTATCATCAGGAGAAAAATCTCATCATAATCATCATGAGTTCGATAGTTCTGAAGAC AAGAGCTGTTCTTCGGTTCACGGTAGGTTTGCGGATATGTGGGGTTTGCGAGAATCATATTCTGATGATGGAGAAAAACA GAGCTCAAGGAAGGTTAGAAGTGGACGGTGGTCGGAGAATGAGATGAGACGAGAACTGAGATGGCTTAAGGCAAGGCACA AGATTCAACTTATGAAAATGAGAGGTCAAACGATCTGCGAGACACCGATAGAGATCTCTCTTACACCGGGAACTTCAGTT TCGTTACCTCTTCTTTACAGGGCTATATCACTTCCTGTGGATGCCGTGGATATGTGACATTATTGTAAAGTCTGATGATA GTTATCACTTATATTTGTATGTTTCGACATTTTTAACTTTTGTTAAGTTAAAAGTTAATGACATAGTGTACTTTAT ....................................i........................................... .........................................................N...................... ................................................................................ ................................................................................ ..............................i...N............................................. .................N...........N...............................................N.. ...N..................N......................................................... .............................................N.................................. ................................................................................ ................N............................................................... ...........N...................N................................................ ..............N...............................N...............N..N...........N.. .........N.....N..........i..............i............................N......... ................................................................................ .............................................................................N.. .....i...N..........i.....................N.....N..N............................ .......N..............N........................N.........................N..N... .....N...................................N..N.............................N..... ....................................................i..................i........ ......................N...................i......N.............................. .....N....................N..................................................... .........................................................N...................... ....................................i...........................N..N............ ......................................N....N................i................... ...........N.....N.............................................................. ........................................N..........N......................N..... ..................N......................................N.................. Pos Score Pred ------------------------ 37 0.708 Yes 138 0.238 - 351 0.700 Yes 355 0.497 - 418 0.241 - 430 0.144 - 478 0.109 - 484 0.170 - 503 0.400 - 606 0.328 - 737 0.385 - 812 0.312 - 832 0.321 - 895 0.081 - 927 0.469 - 943 0.078 - 946 0.273 - 958 0.401 - 970 0.180 - 976 0.122 - 987 0.645 Yes 1002 0.621 Yes 1031 0.293 - 1198 0.073 - 1206 0.733 Yes 1210 0.251 - 1221 0.508 Yes 1243 0.325 - 1249 0.180 - 1252 0.129 - 1288 0.081 - 1303 0.104 - 1328 0.218 - 1354 0.364 - 1357 0.131 - 1366 0.292 - 1402 0.199 - 1405 0.298 - 1435 0.102 - 1493 0.595 Yes 1512 0.780 Yes 1543 0.222 - 1563 0.597 Yes 1570 0.321 - 1606 0.364 - 1627 0.337 - 1738 0.460 - 1797 0.597 Yes 1825 0.248 - 1828 0.244 - 1879 0.383 - 1884 0.210 - 1901 0.528 Yes 1932 0.188 - 1938 0.274 - 2041 0.283 - 2052 0.157 - 2075 0.062 - 2099 0.029 - 2138 0.051 -

Explain the output -- Processing locus 176: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAGTTGAAAACATTTGTAGGTGATGGGCCTCTAGACTCTAAATGGGCTTATTTAGCTAAAAGTTTACAGGGCGGGTTAA TACCAATCTTGGTCGGGTTAACCAACTTGGGTCGGGTGGATGTTCCAATATGAAGAAGAAGACTCGCGCAATCCTAAAAC AGTGAACGATTCGATTACAATGGCGGCCACGTTTCTGCCGGCGACATCTCTCCGTCTAACTCAAAACTCTACTCTCCGCT TCTTATCATTCTTCACCATTTCTAACCCTTCTTATTCTCTCTTCCGTCCTCTACGCCGCCGTGTTCTCCCTCCCTTCGAT GCTTTTCCGGCCAATTCTCGCCGGAGATGCTTTTGCACAGCTGTTTCGGAATCTCTCGGCTCCGGTGATGGAAATAAAGT TGAGAGTTACGAGAAACGATTTGGGAGTAAAGTTGGAGAATTCAGGAAAAAGTTGAGGATAGCTGAAGTGAAAGGAGGAG CGGACGAAGGACTGAGTCGGGTCGGGCAGAGCCTTAACATTATGGGTTGGGTTCGTACACTTCGATCTCAGAGCAGTGTC ACATTCATTGAGATAAATGATGGTTCATGCTTATCGAATTTGCAATGTGTGATGACTTCGGATGCAGAAGGGTATGATCA GGTAGAATCTGGTTCGATCTTGACTGGAGCATCAGTATCTGTACAAGGTACTATTGTAGCTAGCCAGGGAACGAAGCAAA AAGTGGAACTTAAGGTGGAGAAAATCATTGTGGTTGGAGAGTGTGACTCTTCCTATCCGATCCAGAAGAAAAGGGTCAGC CGGGAATTCTTGAGAACCAAGGCTCATTTACGTCCTAGAACCAACACTTTTGGGGCGGTGGCAAGAGTTAGGAATACTCT GGCATATGCTACACACAAGTTCTTTCAAGAAAGTGGATTTGTCTGGGTGGCAAGCCCAATTATCACAGCTTCGGATTGTG AAGGAGCAGGAGAACAGTTCTGTGTCACTACACTTATTCCTAGCTCCCATGAGAACACCGATACTTCCATTGATGCGATT CCGAAAACAAAGGGAGGGTTGATCGATTGGTCTCAGGACTTTTTTGGGAAACCAGCATTCTTAACCGTCTCTGGGCAACT TAATGGAGAAACTTATGCAACCGCTCTCTCAGATGTATACACATTTGGTCCTACATTTCGAGCTGAGAATTCCAACACGT CAAGGCACTTAGCCGAATTCTGGATGATTGAACCAGAACTTGCTTTTGCCGACCTGGATGATGACATGGCCTGTGCCACT GCCTACCTCCAGTACGTAGTAAAGTACGTTCTTGATAATTGCAAGGAAGACATGGAGTTTTTCGATACATGGATTGAGAA AGGAATCATTCGCCGCTTGAGTGACGTAGCTGAGAAAGAGTTTCTACAGCTGGGGTACACAGACGCCATTGAAATTCTTC TGAAAGCAAACAAAAAATTCGATTTTCCGGTGAAGTGGGGGCTGGATCTGCAGAGTGAGCATGAAAGGTACATAACAGAA GAGGCTTTTGGGGGTCGACCTGTGATAATAAGAGACTATCCGAAGGAGATCAAAGCATTCTATATGCGGGAGAACGATGA TGGGAAGACAGTGGCAGCAATGGATATGCTTGTGCCTCGGATAGGAGAGCTGATAGGTGGAAGCCAAAGAGAGGAAAGGC TTGAGGTGTTGGAAGCGAGGCTTGATGAGTTGAAACTCAACAAAGAGAGCTACTGGTGGTACCTCGACCTCCGCCGCTAT GGTTCAGTTCCTCATGCAGGATTTGGACTAGGGTTTGAGAGACTGGTCCAATTTGTCACCGGAATTGATAATATCAGAGA CGTTATTCCTTTCCCGCGCACACCTGCCTCTGCTGAATTCTGATACTCGACCCTCTCATTGCTCAACAGTTTTCTCTTCA TTTCCTCAAAATTCTTGTACTCTTATGTGCCATTATTTATTTGACATAAACGAAAACCTCAA .......................N..................i..................................... .......................................N.........N.............................. ...................i............................................................ ..............................................................................N. ..........................i........................................N............ ................................................................................ .........................................N...................................... ................N..N......i.................i......i.........N...........N...... ................................................................................ ................................................................................ ................................................................................ .....N.......................................................................... ................................................N.......................N....... ................................................................................ ..N...........N.................N............................................... .......................i.................................N..i....i.............. ...................................................i................i........... ................................................................................ ............................................................N................... ...............................................................i............N..N ...................i.....i...................................................... ........................N.....................................................N. .............N.................................................................. ................................................................................ ........................N..................................... Pos Score Pred ------------------------ 24 0.275 - 43 0.631 Yes 120 0.248 - 130 0.135 - 180 0.685 Yes 319 0.418 - 347 0.702 Yes 388 0.405 - 522 0.263 - 577 0.264 - 580 0.418 - 587 0.503 Yes 605 0.500 - 612 0.712 Yes 622 0.369 - 634 0.152 - 886 0.168 - 1009 0.175 - 1033 0.375 - 1123 0.289 - 1135 0.208 - 1153 0.358 - 1224 0.767 Yes 1258 0.171 - 1261 0.599 Yes 1266 0.829 Yes 1332 0.838 Yes 1349 0.742 Yes 1501 0.240 - 1584 0.654 Yes 1597 0.343 - 1600 0.259 - 1620 0.698 Yes 1626 0.623 Yes 1705 0.364 - 1759 0.187 - 1774 0.089 - 1945 0.061 -

Explain the output -- Processing locus 177: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATGTGGTAGATATTTTATTTTCGGGATTGATATTTGGATATCTCTTTATACTGTAAGTGTAAGGTGACGGTACTTAGTT AAGGCTCACTAGTTTCTTTAAATTCTATATAATACGATACCAATTCCTATGATCTTCACTCTCCAAAGACCCAAACCAAA AAAAAAGTTGACATGGACCAAAAAAGCTTTTTCCGGGTTGATTTTTCTCATAGTCACAGCTGGTTTCATCATCTCCTTCA CTTCCACTAACTTCCCTCACGCGCCGACGAGGCTTCTTGATTGCACCGACTCTTCTTCTTCACCTCTTTGCGCTTCGAGA AACTTTCTTTTCAACAAACAACAACCACGACCTATTCCTAAACATGATCCTAAACCAAATACCAAAAACCATGATCACGT GTCCGATACACCAAACCATCCTCTAGACCCACTCACGGTGTCGGAGATTAACAAAATCCGATCAATACTCTCTTCTCACG CGCTATTCACTTCCGGTACACCACACGCGCTCCACACCGTCGTTCTTGAAGAGCCTGAGAAGAATCTTGTCCGTCACTGG GAAAAAGGGAACCCACTTCCTCCGAGGAAAGCTTCCGTCATCGCACGTGTTGGCGCGGACACGCACGTGCTCACCGTTGA TATCTCTACGGGTCGGGTAGATTCAGAGAATAGCCCGGTTCGTGTTTCTGGTTACCCGATGATGACTATAGAAGAGATGA ACGATATCACTGTTGTACCATTTTCAAACGCGGATTTCAACCGTACGATCATCTCTCGTGGAGTTAATCTAACGGACGTG ATTTGTTTCCCAATCTCTTGTGGTTGGTTTGGTAATAAAGAAGAAAACGCGAGGGTAATTAAAAGTCAGTGTTTCATGAC ACAAGGAACACCTAACTTCTACATGCGTCCTATCGAAGGTTTAACCATTCTCATCGATTTAGATACAAAGCAAGTGATCG AGATAACCGATACAGGTCGGGCTATACCCATACCCGGTTCAACCAATACCGATTACCGCTTCCAAAAGCTCGCAACCACC GACAAAACTCGGCCTCTAAACCCGATATCCATTGAGCAGCCACGTGGTCCAAGCTTCGTGATAGAGGACAACCATCTAGT GAAATGGGCAAATTGGGAATTTCATCTAAAACCTGACCCGAGAGCAGGTGTGGTAATATCACGGGTAAGAGTACACGACC CGGATACTCATGAGACACGTGACGTGATGTACAAAGGTTTCGTGTCTGAACTTTTTGTTCCGTACATGGATCCATCGGAC GCGTGGTACTTTAAGACTTACATGGACGCAGGGGAATACGGGTTCGGGTTACAAGCCATGCCACTCGTACCGCTTAATGA TTGTCCACGAAACGCAGCCTATATGGACGGAGTTTTCGCCGCAGCCGATGGAACACCGTTCGTGAGAGAAAACATGGTTT GTATCTTTGAGAGTTACGCCGGAGATATTGGGTGGCGTCACTCCGAAAGCCCCATCACCGGTATACCGATAAGGGAAGTG AGACCAAAAGTGACGCTAGTGGTACGAATGGCAGCTTCGGTAGGTAACTATGATTACATCATTGATTACGAGTTCCAAAC TGATGGGCTTATAAAAGCTAAGGTCGGGCTAAGTGGAATACTAATGGTGAAAGGGACAACATATCAAAACAAAAACCAAG TGGAGAAAGATAAAGACGGTAATGAAGAAGAGCTTCACGGCACGCTTCTGTCTGAAAATGTAATTGGAGTAATACACGAT CACTACGTCACTTTTTACCTTGACCTTGACGTCGATGGCCCGGACAACTCATTTGTTAAAGTGAATCTCAAGAGGCAAGA GACCGAGCCAGGCGAGTCACCGAGGAAAAGTTACCTAAAAGCTGTTAGGAACATTGCGAAAACCGAAAAGGATGGTCAGA TCAAGCTTAGCTTGTACGATCCATCAGAATTCCACGTCATCAACTCTGGTAAAACCACTCGGGTCGGAAACCCGACGGGT TATAAGGTCGTTCCTAGAACGACGGCAGCTAGTCTACTTGACCATGATGATCCGCCGCAGAAGAGAGGAGCTTTTACCAA CAACCAAATTTGGGTCACTCCGTACAATAAGTCGGAGCAATGGGCTGGTGGCTTGTTCACTTACCAAAGCCATGGTGATG ACACTCTTGCAGTTTGGTCAGACAGGGATAGAGACATAGAGAACAAGGATATAGTTGTGTGGTATACACTTGGTTTCCAT CACATTCCATGTCAAGAAGATTTTCCGATAATGCCCACGGTTTCTTCGAGTTTCGATTTGAAGCCCGTAAACTTTTTCGA GCGCAATCCAATCCTCAGTGCCGCTCCAAACTTTGAACATGATCTCCCGGTTTGTGGAGTTCAATCTGTTTCTGCTTGAG AAGAGTAAAGTACGAAATAAAGAAATAGCAACTTTTGTATATTTGTGTTACTCTCAAGGTACACAATGTAATTTGTGGCC TTAAAACTGGTAATTAGA ................................................N............................... ............i................................................................... ................................................................................ ...........................................i..........................N......... ................................................................................ ................................................................................ ................................................................................ ..........................................................i..i..............i... ................................................................................ ...........................................................................N.... ......................i......................................................... ................................................................................ ................................................................................ ...i............................................................................ .........N................N......................................N.............. .....................i...................................i..................N... ......................i........................N.........................i...... ................................................................................ ...........................i.....................N.............................. ..N........................................i.................................... .....................N...................................N...................... ..................................N............................................. .......................................................................N........ ................................................................................ ...........................................N..N................................. .......................................N...............................N.....N.. ................................................................................ ........N.....................i................................................. ......................................i......................................... .................................................................N.............. .................. Pos Score Pred ------------------------ 2 0.177 - 129 0.199 - 173 0.719 Yes 364 0.662 Yes 391 0.272 - 699 0.608 Yes 702 0.680 Yes 717 0.571 Yes 876 0.395 - 903 0.713 Yes 1124 0.774 Yes 1210 0.149 - 1227 0.362 - 1266 0.352 - 1302 0.651 Yes 1338 0.720 Yes 1357 0.428 - 1383 0.681 Yes 1408 0.421 - 1434 0.787 Yes 1548 0.679 Yes 1570 0.353 - 1603 0.318 - 1644 0.685 Yes 1702 0.217 - 1738 0.211 - 1795 0.285 - 1912 0.323 - 2044 0.307 - 2047 0.212 - 2120 0.390 - 2152 0.190 - 2158 0.328 - 2249 0.483 - 2271 0.655 Yes 2359 0.604 Yes 2466 0.064 -

Explain the output -- Processing locus 178: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAGAGGTGTGTAAGCTCCTTCTTGTTCTCTTTATGAGATGTTTGTCAGCATATGCTTCCCATTGTGCTTACACTTTTAA TTTCATCTTTGTTGCACCTGAAGGCAAAAACAATATTTTGAGCAGAGGAGACAGCAACAGCATCAGTTCACAGTTGGGTC TGAGAGCTGTAGTAATGATATAAACAATAGTAATCAACACTTAAGAGAACACCAATCTCTGGATATTCTCAATTTGCTGA ATTTGTCAACCGCTACTCCTGAATGCAAACCTTCTGGTCCAGAAAATGGTAAGTATATGCTTGTTATGACTTCCAGCAGT CATAATTGGAGTCAGTTTATGAACCATTTATTTGCTTTATCGCTCAGAACCATGACATGTAATTTCACTTTGCATTTTTC TTGTTTGTGTCACTTGTTAACGGAAAAAGGAATGCAAGATCTGGATGCTGATTTTTACAGCCTTAAGGACAACATGTCCG GAGTAGGAAGTTCATTTAACCATATAGCAGAACCTACTTCCTCTAAGAGGACTTTGTTTAGTATCCCTGACAACCAGACA AATGATTTCAAGAAAGCAAACACTGACAACCAGACAAATGATTTCAAGAAAACAAACACCACCGCAGATCTGATGGATGG CACTGAGCGGAAGTTATCTGTCTTTGATTTGGTTGGTGATGATCATACAACAACCAACCTGGAAGAATGTTCTCCGTCTG AGGCTCACATGGCATTTTCAGTCGAAGGATTAGGTAAAATAAACACGGAAACTCCAGTTAATTCCCCACAACCATCAGAC AGAACTTTTGTGTACCGGTGCTCCTCTCCATGGAAGGATACTGGTCAACCAGATACATCACATGTCAGGGGAAGGCTCAA CGACTTTGAGAACGAAGTGGACACAATGATTCAAAGTAGCAAAATGTTCCAAGATGACAGCCTTTATAGGTCTCCAATTG GCATACATGCTAAAGATGGGGGCAGAAAGCAAAAACTGCAAACTTTCAGTGATCACTTGCACAAACAATATAGCGACTCT AGAAATTACTTCTGTGACGTTGCAGATTTCAACAACAGCAGGTTCTCTGACGATGAATGGAATGCTAAGCCCGCCTTTCT TGATGACGGGGAAGATAGTTTTTACTGGAAGGCCGAACAGCCATGCCAAAAGGAGAGTTTGAACCCTGATTTCCTGAAAT ATTGCAATGATTGCACTGAGAGTCGCTCTTCTACAGAACACCATAGGAAAAAGAAGAGGGATTACCTGGAAACGACCTGG AGGTCCAACATTAGAGATTCACCAACTCGAAGATCACATCTTTTAAAAAGGAATATCGATTATCCAAGTTTCGCAAAGGC AGCAACCTCCGACTTTGATTTTGACAATGTATTTGACCGACCGGTTTGGTCATCCATTGTACTTGAGGAAGATAAAGACA GCCATAGTTTGCGTAGCGAAGAATCTTGCTCGTCTAGTGCAGTTTGGACCAATGAAACCCACAATTCACAGTTTGAGACC AACACAAGACAAAGGAAAAGGGAAACTAATAAGTTTAGCAACCTTGGAGACAAAAAGTATATCAATACTGACCTTTTCCA AGAATCGTGGGAAGACTGGGAAGTGGATGATCAACATATGAAGAGACAAGTTAGGTCTGGAAAGCAGGGCAGACTATCAA ACTCAGGAAAGTTAAAATCTACCAGTCAGAGAAAGGGTGGTCTAGACGCGTCTTACGATTGGTTTGGAGAAGGATTTACA TCAGCAGGAATAAACTCAGAGATTACAAGTGAGAGGAATAAGCCATATCCTTTCCTAAACCCTGAGCGTGGTTCCTCTCA CTGGCGTTCATCTCGTGCCCCTGATTCTATTCCGGAAACATGGATTCCCAAGTTTTCAGTAGGAGGCACCGGAGATGATG ATGATGGTGACCACGATGAAGAGGACTATGTAAACTGTTTATCAGCAAACCATAAATCTAAACTAGCAGGAGACACATGC GGTTTTGAGAATGACACGTTATCAGAGAACGATAATGAGCAAAGCAGAGAAGTAAATCACCCAAAAAATCAGGGAGATGA GACCTCATCATCCATTGCAAAGAGCCTCTCAGACGAAAATGAGTATATGTAACCCCAACAAGGAGGTCATGGAAGCTCGT CACCAAAGAAACAGAGGTAAATTTAATTCTGTTTGCTAAGATAATTTGTTTATTACCTAGAATTATTACAA .................................N....N.............N........................... ................................................................................ ..............N................................................................. ......................N......................N..........N........N.............. ..................N................................i....N....................... ...............................i............N............................i...... ................................................................................ ......................................N...........................i............. ........i....................................................................... .............................N...............................N.................. .........................N.................i.........N.......................... ......N........i................................................................ ....................................................N...N....N.................. ..N.......................................N..................................... ......i......................................................................... ................................................................................ ..........................N..................................................... ...................................................N............................ ................................................................................ ..........................N..........i.......................................... ................................................................................ ................................................................................ .......................................N..................................N..i.. N..N...........N...........N................................................N... ..........N.......................N.........................................N... ......................................N.......N.....................N........... ....................................................................... Pos Score Pred ------------------------ 34 0.255 - 39 0.342 - 53 0.314 - 175 0.140 - 263 0.115 - 286 0.355 - 297 0.338 - 306 0.285 - 339 0.134 - 372 0.629 Yes 377 0.376 - 432 0.760 Yes 445 0.289 - 474 0.778 Yes 562 0.451 - 598 0.384 - 633 0.769 Yes 637 0.299 - 679 0.137 - 707 0.506 Yes 729 0.604 Yes 830 0.247 - 862 0.419 - 906 0.471 - 924 0.704 Yes 934 0.376 - 967 0.131 - 976 0.701 Yes 1093 0.469 - 1097 0.354 - 1102 0.353 - 1123 0.430 - 1163 0.370 - 1207 0.503 Yes 1387 0.302 - 1492 0.364 - 1627 0.205 - 1638 0.707 Yes 1880 0.488 - 1915 0.456 - 1918 0.634 Yes 1921 0.464 - 1924 0.435 - 1936 0.267 - 1948 0.236 - 1997 0.308 - 2011 0.456 - 2035 0.435 - 2077 0.283 - 2119 0.387 - 2127 0.137 - 2149 0.076 -

Explain the output -- Processing locus 179: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTAAATCGAGTGTCTAATTATTCGTTCCTTCTTCTTCTTCGAGCAACGACAGTCCCTGAGAATCCAAATCGGAAACAA TCTCCGGCAATTCAATAGTAAATGCGTTGCTCAGTTTCCTCTGTTGTTCGTCCACTATCAGTAGATCCTGCGACAGCCAT AGCTACTTTGTGCTCAAAGGGTAACCTTCGAGAAGCTTTCCAGAGATTCCGACTCAACATCTTCACAAACACTTCTCTCT TCACCCCTTTTATTCAATCATGCACCACCAGACAATCTCTTCCGTCCGGAAAGCAGCTCCATTGTCTTCTCGTCGTCTCC GGATTCTCCTCCGACAAGTTCATATGCAATCATCTGATGAGCATGTACTCCAAGCTTGGAGATTTTCCTTCCGCTGTTGC AGTATATGGACGTATGCGTAAGAAGAATTATATGTCATCTAACATACTCATCAATGGGTATGTTCGTGCAGGCGATTTGG TAAATGCCCGGAAGGTGTTTGATGAAATGCCTGATAGAAAGCTCACAACTTGGAATGCTATGATTGCTGGTTTGATCCAG TTTGAGTTTAACGAAGAGGGTTTGAGTTTGTTTAGGGAAATGCACGGATTGGGGTTTTCGCCTGATGAATATACTCTTGG TAGTGTTTTTAGCGGATCTGCTGGGTTGAGGTCAGTGTCTATAGGGCAGCAGATTCATGGCTACACGATCAAATATGGGC TTGAGTTGGATTTAGTTGTTAATAGTTCGTTGGCTCATATGTATATGAGAAATGGGAAATTGCAAGATGGCGAGATTGTT ATAAGATCAATGCCGGTTCGTAATTTGGTTGCATGGAATACACTCATCATGGGGAACGCGCAAAACGGATGTCCTGAAAC CGTGCTTTATCTGTATAAGATGATGAAAATCTCAGGTTGTAGGCCGAACAAGATCACATTTGTAACTGTGCTCAGTTCTT GTTCTGACTTAGCGATAAGAGGTCAAGGTCAGCAGATTCATGCGGAAGCTATTAAAATTGGGGCTAGCTCTGTCGTAGCT GTAGTTAGTTCATTGATCAGTATGTATTCAAAATGTGGGTGTCTTGGAGATGCAGCTAAAGCTTTCTCGGAACGTGAAGA TGAAGATGAAGTGATGTGGAGCTCAATGATTTCTGCTTATGGGTTTCATGGACAAGGCGATGAGGCAATCGAGCTGTTTA ATACTATGGCAGAGCAGACGAACATGGAGATAAACGAGGTCGCGTTTCTGAATTTACTTTATGCGTGTAGTCATTCTGGC TTAAAAGACAAAGGGCTTGAGTTGTTTGACATGATGGTGGAAAAATACGGGTTCAAGCCAGGTTTAAAGCACTACACTTG TGTGGTGGATTTGCTCGGTCGAGCAGGCTGTTTGGATCAAGCCGAGGCAATAATAAGGTCTATGCCAATAAAAACAGACA TTGTTATATGGAAAACATTGTTATCTGCGTGTAACATACACAAAAACGCAGAAATGGCACAGAGAGTCTTTAAAGAGATT CTTCAGATTGACCCTAATGACTCTGCTTGTTACGTCCTGCTTGCAAATGTTCACGCCTCAGCTAAGAGATGGCGCGATGT ATCAGAGGTGAGAAAATCGATGAGAGATAAGAATGTGAAGAAAGAAGCGGGAATCAGCTGGTTTGAACACAAAGGTGAAG TTCATCAGTTTAAGATGGGAGATCGCTCTCAGTCTAAATCTAAAGAGATCTATTCATACCTGAAAGAACTGACTCTGGAG ATGAAGCTGAAGGGATACAAGCCTGATACAGCGTCAGTATTGCATGATATGGATGAGGAGGAGAAAGAATCAGACTTGGT GCAGCACAGCGAGAAACTAGCGGTTGCATTTGCGCTCATGATATTACCTGAAGGTGCACCGATTAGAATAATCAAGAACT TGCGGGTTTGCAGTGATTGCCATGTTGCTTTCAAATATATATCGGTGATCAAGAACCGAGAGATCACGTTAAGAGATGGT AGTAGATTCCATCATTTCATTAACGGAAAGTGTTCTTGCGGCGATTATTGGTAGCTGGGCAGTTAAAGTATGCTATGACT CTCTGCTTCAGAGTCTTTCTGGAGTCTTAAGCACTCAGATTGGATCGGGAAGCCATCTGGGACGAAAGCATTC ................................................................................ .....................i.......................................................... ................................................................................ ...................i............................................................ .......................N............N.....i..................................... .....N.......i.................i.....................N.....N.................... ...N.................N....i...........................N....N.................... .......................................N........................N............... ........................................................N.................N..... ......................................i.....i......N..............i............. .........i......................i...............N...................N........... ...................N..i......................................................... .......................................N........................................ .....................N..........N................i.............................N .....N.......i...........i............N........N...........N.................... .....i.................i....................................N................... ..............................i..i.............................................. .............................................................N.................. .......N.............................................i.......................... ................N.............................N.....................i.......N... ...................N............N............................................... ..............i................................................................. i..........................................N....i...N........................... .....................................N.......................................... .....................N.....................................................i.... .....................................................................N....N..... ......................................................................... Pos Score Pred ------------------------ 102 0.722 Yes 260 0.521 Yes 344 0.223 - 357 0.391 - 363 0.590 Yes 406 0.146 - 414 0.782 Yes 432 0.633 Yes 454 0.417 - 460 0.219 - 484 0.438 - 502 0.148 - 507 0.597 Yes 535 0.168 - 540 0.218 - 600 0.275 - 625 0.101 - 697 0.218 - 715 0.241 - 759 0.523 Yes 765 0.537 Yes 772 0.313 - 787 0.520 Yes 810 0.559 Yes 833 0.648 Yes 849 0.326 - 869 0.485 - 900 0.456 - 903 0.704 Yes 1000 0.092 - 1062 0.355 - 1073 0.371 - 1090 0.500 - 1120 0.361 - 1126 0.167 - 1134 0.727 Yes 1146 0.626 Yes 1159 0.090 - 1168 0.108 - 1180 0.089 - 1206 0.537 Yes 1224 0.593 Yes 1261 0.129 - 1311 0.709 Yes 1314 0.724 Yes 1422 0.273 - 1448 0.446 - 1494 0.752 Yes 1537 0.335 - 1567 0.174 - 1589 0.580 Yes 1597 0.188 - 1620 0.421 - 1633 0.265 - 1695 0.761 Yes 1761 0.791 Yes 1804 0.157 - 1809 0.728 Yes 1813 0.114 - 1878 0.180 - 1942 0.474 - 1996 0.565 Yes 2070 0.134 - 2075 0.102 -

Explain the output -- Processing locus 180: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGAGAAATTTTTGCAAAAAGCTATGTTTTTTTTACGTTCTTCATGGTTGATTTCGATTAAAAGAGATCGCTTTTTGTAG CTCAGGTCTCATCAAATCTGAATCCAATTTTCAGTTGGTGGATATTGGATGATGCTGGTTGTTTCGGAAAACTACTTATT AAGCCCTTCTTTGTACCTTAAAGCTCTTAAATTATGCAGCTACCAAAACGTCAAGAAACAACTATTGTTGATCCATGGGA ATTCGATTACAAATGGGTTCTGCTCCAATCTCCAGTTGAAGGACATGTTGATAGATTTGTACTTAAAACAGGGAGATGTG AAACATGCACGTAAACTGTTCGATAGAATTTCTAAGAGAGATGTGGTTTCTTGGACAGCTATGATCTCGAGGTTTTCTCG ATGTGGATATCACCCGGACGCGTTGTTGTTGTTTAAAGAGATGCACCGGGAAGATGTTAAGGCTAATCAGTTCACTTATG GGAGTGTTTTGAAGTCGTGTAAAGATTTGGGGTGTCTGAAAGAAGGGATGCAGATACATGGTAGTGTGGAGAAAGGGAAT TGTGCAGGGAACTTAATTGTGAGAAGTGCATTGCTTTCTCTTTATGCCAGGTGTGGAAAGATGGAGGAAGCTCGTCTTCA GTTTGATTCAATGAAAGAAAGGGATTTGGTTTCTTGGAATGCTATGATCGATGGATACACCGCCAATGCTTGTGCAGATA CTTCGTTTAGTCTGTTTCAGTTGATGCTCACGGAAGGGAAGAAGCCAGACTGCTTCACCTTTGGAAGCCTCTTGAGAGCT TCTATTGTCGTTAAGTGTCTTGAAATAGTCAGTGAATTACATGGATTAGCGATCAAGCTGGGTTTTGGGAGATCAAGTGC ACTAATTAGGTCTCTGGTTAATGCATATGTGAAGTGTGGTAGCCTTGCCAATGCTTGGAAACTGCACGAGGGCACAAAGA AAAGAGACTTACTATCTTGCACTGCTCTAATTACAGGTTTTTCACAGCAGAACAACTGCACAAGTGATGCCTTTGATATC TTCAAAGATATGATACGGATGAAAACAAAGATGGACGAAGTTGTAGTATCCTCGATGCTTAAAATATGCACCACCATTGC ATCTGTAACCATTGGAAGACAGATCCATGGTTTTGCCTTAAAATCTTCCCAAATCAGATTCGATGTTGCTTTGGGGAATT CGCTGATAGATATGTATGCAAAGTCTGGGGAAATCGAGGATGCAGTCCTGGCTTTCGAAGAGATGAAAGAGAAAGATGTG AGATCATGGACCTCTTTGATTGCAGGATATGGAAGACATGGGAATTTTGAGAAAGCAATTGATCTTTATAACAGAATGGA GCATGAAAGGATCAAACCGAATGACGTTACATTTCTTTCTCTCCTCTCTGCTTGTAGTCATACAGGACAAACCGAACTAG GCTGGAAGATTTACGACACAATGATCAACAAGCACGGAATCGAAGCTCGAGAAGAGCACTTATCTTGCATCATAGATATG CTTGCGCGCAGTGGCTACTTGGAAGAAGCTTATGCACTGATAAGAAGTAAAGAGGGTATTGTAAGTCTTAGCTCATCAAC ATGGGGAGCTTTTCTTGATGCATGCAGGCGCCATGGGAATGTGCAGCTCAGTAAAGTAGCAGCCACGCAGCTTCTGAGTA TGGAACCGAGGAAACCAGTCAATTACATCAATTTGGCCAGTGTGTATGCAGCTAATGGAGCATGGGACAATGCTTTGAAT ACTAGAAAGCTTATGAAAGAGAGTGGTTCCTGCAATAAAGCTCCAGGATACAGCCTTGTGTATTGAACTAAGAGAATAAG ACAAAATCTCCAGAAAGGGTATACACTGTGGTACAAGGGTGGACGTTAACAAGGCTACAAAGAGCATCTCAAGGTCTCAG GGAGT .......................N...................N.................................... ................................................N..N............................ .................................i........................................N..... ............N...............................N..............................N.... ....i...................................N...................N................... N.......................................N............N.......................N.. ...............................................N.........N...................... ...........................................N................i................... ..........N...........................N....N......N..............N.............. .......................N........................................................ ........................................N....................................... ....................N.....N.......................i............................. ..................................................................N............. .........N........N...........i.......................N..........i.............. ..........................N...................................N................. ...........i...N.......................N......................i............N.... .....N......................N........N.....................................i.... ..N.................i........................................................... ....................i........................................................N.. ...............................N................................................ i................N...N..........N.....N........................................i .............................................N........N......N.......i.......... ............N................................................................... ................................................................................ ..... Pos Score Pred ------------------------ 24 0.247 - 44 0.321 - 129 0.164 - 132 0.248 - 194 0.584 Yes 235 0.416 - 253 0.448 - 285 0.132 - 316 0.451 - 325 0.513 Yes 361 0.192 - 381 0.351 - 401 0.199 - 441 0.362 - 454 0.365 - 478 0.321 - 528 0.378 - 538 0.331 - 604 0.295 - 621 0.874 Yes 651 0.316 - 679 0.301 - 684 0.490 - 691 0.319 - 706 0.156 - 744 0.358 - 841 0.434 - 901 0.115 - 907 0.317 - 931 0.542 Yes 1027 0.152 - 1050 0.362 - 1059 0.280 - 1071 0.675 Yes 1095 0.300 - 1106 0.612 Yes 1147 0.284 - 1183 0.070 - 1212 0.558 Yes 1216 0.260 - 1240 0.300 - 1263 0.503 Yes 1276 0.440 - 1286 0.429 - 1309 0.159 - 1318 0.185 - 1356 0.577 Yes 1363 0.334 - 1381 0.661 Yes 1461 0.775 Yes 1518 0.360 - 1552 0.348 - 1601 0.507 Yes 1618 0.326 - 1622 0.432 - 1633 0.326 - 1639 0.206 - 1680 0.506 Yes 1726 0.391 - 1735 0.226 - 1742 0.282 - 1750 0.502 Yes 1773 0.142 -

Explain the output -- Processing locus 181: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACTATTTTTGATGGCAGATTAATTTAGTCTTGCCCCATATCTCGGTTCTGCTAAATTGGTTATTGTTGGTTTAGTTTAG ATTCGGTTTAAGTTTTCTTAAAGAGGAAATTTTAGAGAATATATGAAAAAGAACCAAAATAAAAAGAGGGGCAAAAGTGT AAATTCAAGTATTTCCTCGGCAGGCTGCAAAAAGCAGTGTTTGAGGCTCTGTTACGATATTAATGGCGAAAAAACGACAA AAGAGACAAACACTACACACAGCCTCCGTACGTACACTCACGCAACAACATTCTCTCTCTCTCTCTCTCTCTCACAAACC ATTTCTTGTTATATCACCAAACCTCTCCTCCTCTCTCCGTATTGTTTCCTTGGCTTTACTCTCTCTTCTCTCTCCTTCTT CGTTTGTAACCTTTACTCTGTTTTCATGTTCGGAGAGAGATTCATGGGTTTTGGAGGTTTTAATGGAGACTTCAACTTCC GAGGAGCTTCCGATCATCGTCCTCTTGGTTCTGATGGGTTTTTACCGAGTCTCGACACAAACCCATTTCTGAAAAACCAC AAGAGTGTTGAAGCTCTGGATTTGTGTAAAAAGCTTCACAAAATGGGTATCTCTTGTGACATGAGTATCTGGACCAAACC CGAAGAACAGTTCCGGGTCGACCCGAGTGAGTTTGGGGCTAGAACTCTTCATGGGTCTTCTGGGTTTGACCAGAATCTCA CCGGAGCTTCTCAGATTCATGATGGGTTTCGTAACTTTTCCTCTGTTCGTCTGCAAAACAACAATTTTCATGGAGTTTCT CCATCTCCGGGAGAGATGAGATTACTTGGCCGTCAAGATTCCTTTAACCTAAACGGATTTGAAGAGATGTTAGCTCTTAA GAATCACAGAGATTTTCTCTTAGATCAGATCCATGAACCCATAAAGCGTCCTCCTTTTCTCAGAGGCAACGATGCTCTCA TAAGTTCGTTGATGTTTGAAGGAAATAATAGGGTTTCTCAAACCCTAGCCGCCATGGAAGCTTCTAGAGGTTTTTATCCA GAGGAGGATAGTTCTCTGCTGAGTCCATTTCACGAGAAGGTTTCTGGAAAGCTTGGAGCTTCTTATCTAGAAGATACAGT ACTAATCGGTCAAGGCTCTTATGGGAAGATGAGTCCAAAAAGCAATAATGATCTCGTCTCCATGGTTGAGATTTATGGAT CTGTGAACTTAATGGCGAAAGATCAGATTGGTTGCAGAGTTTTGCAGAAACTGGTCGAAGAAGGAACATTTCATGAAGCT AAGGTTATATTACTTGCAATCATTGACCATGTCGTTGAACTTTCCATGGATCCTTTTGGGAATTACATTGTTCAGAAGCT GTTTGATGTGAGTGATGAGGAACAGAGGACGTTGATTGTGAGTGTGTTAACGTCAAATCCAAGGGAGCTTATCAGAATCT GTCTTAACACTTATGGGACAAGGGTTGTGCAGAAGATGATTGAAACTGTGAAAACAAAACAGCAGATTGCATTGGTGAAG TCAGGTCTCAAACCGGGGTTTCTTGCTCTTGTCAAAGATCTGAATGGGAACCATGTGATACAGAGTTGCTTGCAAACCCT TGGCCCTAATGACAATGAGTTTGTGTTAGAAGCTGCTACTAAGTACTGTGCTGAGATTGCTATTCATCGACACGGATGCT GTGTTCTTCAATGCTGCATTTCAAACTCTGTTGGACTGCAACGTGAAAGACTCGTCGCTGAGATATCAAGAAACTCGCTT CACCTTTCTCAGGATCCTTTTGGGAACTATGTGGTGCAGTACTTAATAGATCAACAAGTTTCTGCAGTGAAGTTGCTGGT TCAGTTTAGAATGCACTACGCCGAGTTAGCTACTCAGAAGTTTAGTAGCCACGTGATTGAGAAGTGTCTAAGGAAGTATC CAGAGAGTCGAGCTGAGATCGTCCGTGAGCTCCTCTGTGTTCCAAACTTTGAATATCTTCTGCAAGATCCTTATGCGAAC TATGTGATCCAAACTGCTCTCTCTGTAACCAAAGGACCTGTTCGTGCTAAATTGGTGGCGAAGGTTTACAGGTACGGGAA ACTACACTCGAGCCCTTACTGCAAGAAGATTTTCTCCAAGACCATCTTGAAGAAGTGACTTTGATTAGTAACTAAAAAAT GTTGTTGTTGTTATTTTGTGTTTCAAGATCGGATAGGGCTTTAGAGAAAACAATGCCCTTAACTTTTTTGTTGTTGG ...........N.................................................................... ..........................................N..................................... ..............................................................N................. ................................................................................ ................................................................................ .........................N.................i..................i................. .................................N.............................................. ..........................................i.................N................... ..................................................N............................. ..................N..N...............................................N.......... ...............i..................................................i............. ................................N......................................N........ ...........i.........................................i.......................... ................................................................................ ....................N.......i..................i.............i............N..... ...........N............................................................N....... ............................N................i.................................. .....N........N................................................................. ............N......................N............................................ ...........................................N........N........................... ........N.....N............................................................N.... ..........N..................................................................... ............................N................................................... ..........i..................................................................... ........................................................................N....... ..............................................................................N. ....................................................N........................ Pos Score Pred ------------------------ 12 0.460 - 123 0.320 - 223 0.212 - 426 0.484 - 444 0.882 Yes 463 0.640 Yes 514 0.080 - 603 0.621 Yes 621 0.387 - 691 0.321 - 739 0.342 - 742 0.223 - 790 0.356 - 816 0.581 Yes 867 0.734 Yes 913 0.271 - 952 0.354 - 972 0.636 Yes 1014 0.741 Yes 1141 0.103 - 1149 0.735 Yes 1168 0.531 Yes 1182 0.692 Yes 1195 0.103 - 1212 0.469 - 1273 0.133 - 1309 0.321 - 1326 0.661 Yes 1366 0.188 - 1375 0.249 - 1453 0.108 - 1476 0.482 - 1564 0.125 - 1573 0.418 - 1609 0.199 - 1615 0.445 - 1676 0.184 - 1691 0.284 - 1789 0.201 - 1851 0.618 Yes 1993 0.222 - 2002 0.483 - 2159 0.108 - 2213 0.053 -

Explain the output -- Processing locus 182: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTTCGTGATTCTTTAGTTCCTTGATCTTCTTGGGATTTCATTTTTCGTTTCTCTTTAGATTACTGCTTCTTCTTTTTTT TGTTAGGTTTGTTAGAGATAACGAGAAATGGGATCTGTTAATTGTTGTAGGGTTCGTAGTTCGTTGTTAATGGCGGGTTG GTCGATGGTAGCCGACGAGGATGCCGTCGATTATAGCAAGACTCCAAAGAGTCTCGATGCGAATAGGTCTCCTGAAAGCG TTAACCCAGAATCTACAGGGTTTGAAAAGAAGATTAAAGAACTCATCTCCTTGTTCCGACCTTTGCTTGATTCTTTTCTC GCTGAGTTTTGTTCGGCGGATGGTTTTCTACCTCTTCCTGCGATGACCGGTGAAGGAAGGACCGTTGATTTGTTCAATCT CTTTTTGAACGTGACTCACAAAGGCGGATTCGATGCAGTTTCTGAGAATGGGTCATGGGATGAAGTTGTTCAAGAGTCTG GATTAGAGTCTTATGACTCTGCATCTGCGAAATTGATCTATGTTAAGTATCTTGATGCATTTGGTCGATGGTTGAACAGA GTTGTTGCAGGAGATACTGATGTTAGTAGCGTGGAGTTGTCTGGAATCTCTGATGCTTTGGTGGCTAGACTTAACGGTTT TTTATCTGAAGTGAAAAAGAAATATGAGCTGAGGAAAGGTAGACCTGCAAAGGAGTTAGGAGCAGAGCTTAAATGGTTTA TTTCGAAAACGAAGAGAAGATATGATAAGCACCATGTTGGCAAAGAGTCTGCTTCGAATGATGCTGTGAAAGAGTTTCAG GGATCAAAATTGGCGGAGAGGAGACTGGAGCAGATAATGATCCTTGAATCAGTTACTCAGGAATGCTCTTCTCCTGGGAA GAGAAAACGAGAATGTCCTTTGGAGACATTGAAATGGTTAAGCGATGTTGCTAAGGATCCATGTGACCCATCTCTTGGTA TTGTGCCAGATAGATCAGAGTGGGTATCTTATGGAAGTGAAGAGCCATGGAAACAACTTCTTCTGTTTAGGGCCTCAAGA ACAAACAATGATTCAGCTTGTGAAAAGACTTGGCAGAAAGTCCAGAAGATGCACCCATGCCTGTATGATGATAGTGCTGG AGCTAGTTACAATCTAAGAGAGAGACTGAGTTACGAGGATTACAAGAGGGGAAAGACTGGGAATGGCTCGGATATAGGCA GTTCAGATGAGGAGGATAGACCATGTGCTCTAGTTGGGTCAAAGTTTCAAGCTAAAGTACCGGAGTGGACTGGCATAACC CCTGAAAGTGATTCAAAGTGGTTGGGCACTCGGATCTGGCCACTGACCAAAGAACAAACCAAGGCTAATCTTCTTATCGA AAGAGATCGTATTGGAAAAGGAAGACAAGATCCTTGTGGCTGCCATAATCCAGGATCTATTGAATGTGTCAAGTTTCATA TCACTGCAAAACGGGATAAACTGAAACTTGAACTTGGTCCAGCTTTCTACATGTGGTGTTTTGATGTCATGGGTGAATGT ACTCTCCAGTATTGGACAGACTTAGAACTGAAGAAGATAAAGTCTTTGATGAGTTCTCCACCGTCACTTAGCCCAGCCTT CATCCATCAGGCCAAGATGATTCTTCCTTCGAAGAGCAGAGGAAAAATTGTGAGCTACTTCTACAACGTGACCCTTTTGC AGTACAGAGCAAGTCAGAGTAGAATAACACCTCACGATATAGACAGTGATACTGATCAAATATTCCTCCGCGCAACAGAG AATGAAAATCCAGCCCCCGAAGCAAACACATTTCAGAAACCCGCCCTGCTTTCGCCTAATAAAAAAAGATGTCGATAGAG CTATCACCCGGTTGAGATTCAAGTAGGAGCAGCTCTTTTAGTTTTCTTCGTTTTTGGGGCTTCTATTGCCATTGAAACTG CTTTAGACTCTGTGGGG ................................................................................ ...........................i.........................................i.......... ....i...............N...................................N....................... ................................................................................ ...................N......................i..................................... ................................N..............N......N....N.................... ............N..........................N..............N............N............ ...................N................................N........................... .......................N................................................N....... .....................N...........N.......................N..N................... ....................................N.........................N................. ............N....................i..........N...............N................... ..............................N...............i................................. .......i........................................i.......N.......N..N............ ..............................................................N................. ......N...............i......................................................... ................................................................................ ...............................................................N................ ..................................................N............N....N.......N... ................................................N............................... ................i............................................................... ................................................................................ ................................................................................ ................. Pos Score Pred ------------------------ 108 0.858 Yes 150 0.856 Yes 165 0.692 Yes 181 0.341 - 217 0.079 - 340 0.165 - 363 0.820 Yes 433 0.183 - 448 0.207 - 455 0.343 - 460 0.284 - 493 0.249 - 520 0.079 - 535 0.112 - 548 0.172 - 580 0.208 - 613 0.100 - 664 0.233 - 713 0.261 - 742 0.292 - 754 0.297 - 778 0.087 - 781 0.135 - 837 0.228 - 863 0.262 - 893 0.285 - 914 0.603 Yes 925 0.375 - 941 0.251 - 991 0.441 - 1007 0.622 Yes 1048 0.617 Yes 1089 0.761 Yes 1097 0.320 - 1105 0.238 - 1108 0.368 - 1183 0.492 - 1207 0.397 - 1223 0.520 Yes 1424 0.201 - 1491 0.264 - 1504 0.291 - 1509 0.450 - 1517 0.407 - 1569 0.475 - 1617 0.517 Yes 1762 0.251 - 1829 0.370 -

Explain the output -- Processing locus 183: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTACCTCTGATCATGGCATAGCACACAACAAAATAGTTTATTCATAAAAGTAAATATAGTTTATTCATAAAATACAAAG ATTTCCAATCACACAATGTAACAAGCACAAATAAGTATACTTTTACTTATAAGAAGACATCGGATGTACGTAGACAAACA AGTATCAAAGTATGTTCATAAAGGAAAACAAAGTTCGCATTCAACAAGGTGGGTTAATACCTTCACGCATGCACAAGTGA TGGAAAGCAATACAAATACTCAGATCAACAAAATGACAACTTTTTCAGCAAGCAATACAAATATACAATATAAAAAATCT GGGGTTTCTTGGTTGGGCTTGAATTTGAGAAAGGCCCAGATCCTTCCAGATACTCTTTATTTGACTAATAAATATCACAA AAACAATTTTCTCTTAAAGCTTTTCTTGAAGAACAAGAGAAATCAGAAGCTCTTTCCTTTGTTTTTTTTTTCTTTTTCTT CCACCAATCTACAGATTTCGAATCATCAGAAAATGGATTTCGAGCTTAGATCAGCGAAAGAGAAGCTTGAAAGAGAGCAG AGAGAGAGGAAGCAAAGAGCGAAACTGAAACTCGAACGCGAGAAGAAATCAAAGGAAGCTGCGATTAAGCAACGCGAAGC CATTGAAGCTGCTCAAAGAGCTAGAAGACTTGACGCCATCGAAGCCCAGATTAAGGCTGATCAACATATGCAAGAGAGTT TAGTATCTGGAGATGGAATAGTGTTTGAACGAGTCTTCCAAGCTGTTTCTTTTCAAGGAAATGGAGATAAGATTAAACTA CCACCATCATGTTTTACTGAGTTGTCTGATCAAGGAGCTTTCGATAAAGGTCCTTTGTATTTTGAGCTCTCTGTTGTTGA CCATGCGGATAATAAAAAGACAACACATTCTGGTGTTCTTGAGTTTACTGCTGAAGATGGCACTATTGGACTTCCTCCAC ATGTGTGGAGCAATTTGTTTTCAACGCATGATCCAATGGATGTTCCTTTGGTGGAAATTCGGTATATCCGATTGCCTAAA GGCAGTTACGCGAAGCTTCAACCTGATAACCTTGGTTTCTCGGATTTGCCTAACCATAAAGCAATACTTGAAACTATTCT TCGTCAACATGCTACACTTTCTTTAGACGATGTTCTCTTGGTTAATTATGGGCAGGTCTCTTACAAGCTTCAGGTTCTTG AGTTAAGACCTGCCACTAGCATTTCGGTTCTGGAGACTGACATTGAGGTTGATATTGTTAGTCCAGATATAGTTTCAGAT CAACCGAATCAGCATGTGCTAAAGCCGCTTCAATATGGGAAATCTGAATCTGGAACAGTTGAGGAAGGTCGGTATGATTA CTATAAGTTTGTGATTGACGAGGCTACTGTAGAAAAAGTAATGGCAGGAAGCGTCAAAGTTATTGTAAAGGTAGATGTGG AGAAAGTTGGAGCGGATACGGATCTCTATGTGTCAAAGCACCCGGTTCTATTTCCATCTCTTAATCAACACGAGTGGTCT TCACACGACGTGGGTTCAAAGACCTTGATCTTGGTATCAAAGGAGAGGGCTTTGAGCTCAGGGACTTACAGTATAGGTGT TTACGGCTTCAAAGGAACAGTCAAGTACCAGGTCTCAGTACTGGTCCAAGAAAGCATTGATGGAGCTAAGGTTGGGGAAC GGGCTGTATCATCTTCGTCAGATGTTGATACAGTAGAATGTAGGAACTGTAAGCATTCAATTCCAAGCAGGAGTATCGCG TTGCACGAGGTGTACTGTAGCAGACACAACGTGGTTTGTAACCATCACGGGTGTGGAATCGTTCTCAGAGTCGAAGAGGC GAAAAACCATTTGCACTGTGAAAAATGCGGGAAAGCATTGCAGCCAACAGAGATGGAGAAACATTTAAAAGTTTTCCATG AACCACTCACTTGTGGCTGCGGAATAGTACTTGAGAAAGAACAGATGGTTCAACATCAAGGGAAAGATTGTCCTCTTCGC CTAATCGCGTGTAGATTCTGTGGAGATATGGTGGAAGCGGGTAACTCTGCAGCTGATACTAGAGACAGGATGAGAGGAAT GTCTGAACATGAGAGTACTTGTGGTTCAAGAACTGCACCATGCGACTCATGTGGTCGATCTGTGATGCTCAAAGACATGG ACATTCACCAGATTGCTGTTCATGGCAAGAGTAGCTGATTTCTGTATCCGGTTTCCACCAATTGTTATTGAAAACTGATT GTAATATTGAAAGTGTCAAATGTTTGAACAGAGAAGCATCGAACCATTTGTATTATT .............i.................................................................. ...............i...............................................N................ ...........N........................................................N..........N ................................i............................................... ................................................................................ ................................................................................ ................................N............................................... ................................................................................ ...................................................................N............ ............N...............................................N................... ........N....................................................................... ..N.....................................................N....................... N..........................N.......i...N........................................ ................................................................................ ........N....................N.................N................................ ................................................................................ .............N....................N......................................N...... ........................................N.................................N..... ...........................N.................................................... ................................................................................ ...........................................................N.................... .....................N...............i.......................................... ................................................................................ ........................i...........................i........................N.. ............................................i................................... ...........................i.........................................i........i. ........N..............................N........N...............N...........i... .....................N.......................................................... ...................N..................................... Pos Score Pred ------------------------ 14 0.617 Yes 96 0.611 Yes 144 0.348 - 172 0.442 - 229 0.168 - 240 0.303 - 273 0.536 Yes 513 0.461 - 708 0.285 - 733 0.409 - 781 0.109 - 809 0.203 - 883 0.122 - 937 0.280 - 961 0.244 - 988 0.162 - 996 0.604 Yes 1000 0.178 - 1129 0.167 - 1150 0.427 - 1168 0.155 - 1294 0.228 - 1315 0.356 - 1354 0.238 - 1401 0.433 - 1435 0.304 - 1468 0.212 - 1660 0.306 - 1702 0.378 - 1718 0.665 Yes 1865 0.600 Yes 1893 0.712 Yes 1918 0.417 - 1965 0.827 Yes 2028 0.774 Yes 2070 0.537 Yes 2079 0.770 Yes 2089 0.232 - 2120 0.301 - 2129 0.104 - 2145 0.403 - 2157 0.653 Yes 2182 0.337 - 2260 0.143 -

Explain the output -- Processing locus 184: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTCTTCTTTTAGCTTAAATGTCTCTGGTTAGTTATGTCTGAAGTATACAACACCGGTTCCAAAGTTCGTTGACCATTG TTTGAGTTCTTCTTTTATTAGGATTGTGAGAATAAGAATCGGATTATGCTAACTCTAATTCGTAATCTGCGTTTTACTTT AGGTTACTCAATGAAGCAATTCATGTCAGTGGTGAGATTCAAATTTGGTTTCACTTCAGTCAGAATGAGGGATTGGTCGG TGGGAGTCTCCATTATGGTTCTTACATTGATCTTCATCATCCGTTATGAACAATCTGATCACACTCACACTGTGGATGAT TCTAGTATAGAAGGAGAGAGTGTTCATGAACCCGCAAAGAAGCCACATTTTATGACTTTGGAAGATCTTGATTATCTATT TTCAAACAAGAGCTTTTTTGGAGAAGAAGAAGTGTCCAATGGAATGCTTGTATGGTCTCGAATGCGTCCATTTCTTGAAA GGCCAGATGCTTTGCCAGAAACTGCTCAAGGGATAGAAGAAGCTACATTGGCAATGAAAGGTTTGGTTTTAGAAATCAAT AGAGAGAAGAGAGCTTATTCTTCTGGTATGGTCTCTAAGGAAATTAGAAGAATCTGTCCGGATTTTGTCACTGCATTTGA TAAGGATTTGTCTGGTTTAAGTCATGTACTTCTTGAGCTTCCTTGTGGTTTAATTGAAGATTCTTCAATAACTTTGGTTG GTATTCCTGATGAACATTCTAGTAGCTTCCAGATTCAGCTCGTTGGCTCGGGATTATCAGGAGAAACTCGTCGGCCAATA ATCTTGCGTTACAATGTGAACTTTTCTAAACCATCGATAGTGCAAAATACATGGACAGAGAAGCTAGGTTGGGGAAACGA AGAGCGATGCCAATATCATGGATCATTGAAAAATCATTTAGTTGATGAACTTCCTCTCTGCAACAAACAGACCGGTAGAA TCATTTCGGAAAAGAGTTCCAACGATGATGCAACTATGGAACTTTCTCTTTCAAATGCTAATTTTCCATTTCTCAAAGGG AGTCCCTTCACTGCCGCATTGTGGTTTGGCTTAGAAGGTTTTCATATGACGATAAATGGGCGGCACGAGACTTCATTTGC TTACAGGGAGAAGCTCGAGCCATGGTTAGTCAGTGCAGTCAAAGTCTCAGGTGGTTTGAAAATTTTATCTGTCTTAGCCA CAAGACTGCCCATTCCCGATGACCATGCATCTTTAATCATAGAAGAGAAACTTAAAGCTCCATCTCTTTCCGGGACAAGA ATAGAACTATTGGTGGGTGTTTTCTCCACTGGAAATAATTTTAAGCGGCGTATGGCATTGAGAAGATCTTGGATGCAATA CGAGGCAGTAAGATCTGGCAAAGTAGCTGTTCGATTTCTCATTGGCCTTCACACAAATGAAAAAGTCAATTTAGAGATGT GGAGAGAATCTAAGGCATATGGAGACATTCAGTTTATGCCATTTGTTGACTACTATGGTTTACTTAGCTTGAAAACAGTT GCGCTTTGCATTCTCGGGACCAAAGTCATCCCAGCAAAATACATAATGAAGACGGATGATGATGCGTTTGTACGGATTGA TGAGCTCCTATCAAGTCTAGAAGAAAGACCGTCTAGTGCCCTTCTGTACGGTTTGATCTCATTTGATTCATCACCGGACC GTGAACAAGGCAGCAAATGGTTTATCCCTAAAGAGGAATGGCCTTTAGATTCATACCCTCCATGGGCACATGGCCCTGGC TACATCATCTCTCATGATATAGCGAAATTTGTGGTGAAGGGTCACCGTCAAAGAGATCTTGGAGTGAGTTTTGTAGTTTC ATCACCATAGAGTTAAGATATAACGAATCCTAGATATCATAAGTGACGTTGTTCTTATGTTGTTTGTTGAAAGCTTTTCA AGCTGGAAGATGTGGCGATGGGGATATGG ...................N...............i............................................ .............................................N.................................. ..........N...........N.........................................i............... ..............i..............................N.............................N.... .........................N.........................N............................ ......................................i....N.......N.........i.................. ......N..............................................i.......................... ...........................N.................................................... .......................N........................................................ .........N...................................................................... .............i....................................i............................. ......N..........N..........................N................................... ........................N..N.......i..................N......................... .............................................i.........i........................ .....................N.......................................................... ..................N.....N....................................................... ...................................................N....................N....... ........................................................N...................i... ..................N................N..................N......................... .............................................i.........N..N..i.................N ................................................................................ ................i....................N.......................N.......N.......... .............N.................................................................. ........................................................N....................... .........N.......N.......N... Pos Score Pred ------------------------ 20 0.399 - 36 0.536 Yes 126 0.499 - 171 0.211 - 183 0.302 - 225 0.651 Yes 255 0.621 Yes 286 0.164 - 316 0.061 - 346 0.167 - 372 0.268 - 439 0.524 Yes 444 0.497 - 452 0.471 - 462 0.563 Yes 487 0.135 - 534 0.668 Yes 588 0.412 - 664 0.121 - 730 0.142 - 814 0.538 Yes 851 0.604 Yes 887 0.250 - 898 0.257 - 925 0.079 - 985 0.322 - 988 0.234 - 996 0.552 Yes 1015 0.141 - 1086 0.546 Yes 1096 0.619 Yes 1142 0.424 - 1219 0.156 - 1225 0.251 - 1332 0.422 - 1353 0.334 - 1417 0.236 - 1437 0.535 Yes 1459 0.394 - 1476 0.364 - 1495 0.263 - 1566 0.780 Yes 1576 0.171 - 1579 0.420 - 1582 0.633 Yes 1600 0.327 - 1697 0.510 Yes 1718 0.448 - 1742 0.360 - 1750 0.269 - 1774 0.154 - 1897 0.068 - 1930 0.040 - 1938 0.068 - 1946 0.035 -

Explain the output -- Processing locus 185: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTATTGAGGTTATCTCTTCTTACGGAGACTTATCTGGCAGGTCTTTTGTGGCCTAAAGCTCATAAGTTTATGCTAGTG CAAGAAGTTATATAGGAATGACATATTAGATTTACAATGATGGAAGTGGGGTTCTCTCTTATTCAGTGGTTGATCAGTTC TGGTGCTGACAGTCCGTTTATATTTGGATGGCTTGTAACTGGATCTGTTGGCCTTCTGGCTGTTATCTACACATTTCTTA AATGGCAGAAAAAAACTTCTTTAAATTGGGTTAAAGCTGCTGCTAGAGAGAAGAAGAAGGTATGGAAAAGACTGAGAGTT CCACTTTCTCATCATCAATGGACTGATGATTATGGTTATGGGCAACAGCCATCCACTTGCTGTGTTTGTCTCTACTCCCT TGTTCCAGGTCAAAATGTTTCTAATAAAGCATCTCTGAGTATTCCCGTCCACCGATGTGCTGTATGCGGTGTAGCAGCTC ACTTTTATTGCTCAAGCTCTGCAGCAAAAGACTGCAAATGTGTTGCACAGGCTGGGTCTGACCATGTCCGACATCACTGG TCTGAGCGATGGGTTAACATGGATGATAATGCTGACATGACTGCCTTTTGCTTCTACTGTGATGAACCTTGTGGTATTCC TTTCATTGAAGCTTCTCCTATGTGGCACTGTCTTTGGTGCCAGCGCCTCATACATGTTAAGTGTCACATGATTATGTCCA AGGAATCTGGTGATGCTTGTGATCTTGGCTCTCTCAGAAGAGTAATCCTCTCTCCAGTACATGTAAAACTCAATGAAGCA AATGGTGTCGATGGAGTACTGACTACTATTAAAAACGAGTTAGCATCTATACGAGGGCATGTGAGAAGAAAGCGCCACAG GGGAAAAAATGGGAATGGCCAATCTTTAAATGGTAAGCTACTGGAGGATTCAGTCAGCGATCCAGTAAAAACCGTAGTTA ATGGGCTTGTAGTGAAGAAACTACGCAGAGATAGGAGCATAGATTGCTTAAAGCAAGTCTCGGACATGCCTAATGCCAAA GGGTTGCAAAATGGTATAGGCGGGCACAAACGAAATAAAAGTGCCGCTTTAAATTTTATGAAGAAGTTTTCCTTGGTTGA TTTGCCCCCAGATGCAAGACCGCTTTTAGTCTTTATTAACGCCAAAAGCGGAGGTCAACTCGGTCCTTTTCTTCACAGGA GACTTAATATGCTATTGAATCCAGTACAGGTCTTTGAACTTGGCTCTTGTCAAGGGCCAGACGCTGGTTTAGACCTATGT AGCAAAGTGAAGTATTTTAGAGTTTTGGTGTGTGGAGGAGATGGAACAGTTGCATGGGTGCTTGATGCTATTGAGAAGCG CAATTTCGAATCCCCTCCACCTGTCGCTATTCTTCCTTTAGGCACAGGAAATGATTTATCTAGAGTTTTGCAGTGGGGAA GAGGTATTTCAGTAGTTGATGGACAAGGAAGTCTAAGAACGTTTTTGCAAGACATTGATCATGCTGCAGTTACAATGTTG GATCGATGGAGTGTTAAAATTGTAGAAGAGAGTACAGAAAAATTTCCAGCAAGAGAAGGCCATAAGTTTATGATGAATTA CTTAGGTATTGGCTGCGATGCAAAAGTTGCATATGAATTTCATATGATGCGGCAAGAGAAACCTGAGAAGTTTTGTAGTC AGTTTGTAAATAAATTACGATATGCTAAAGAAGGTGCGAGGGATATAATGGATCGAGCATGTGCCGATTTACCATGGCAA GTTTGGCTTGAAGTTGATGGAAAAGACATTGAAATTCCTAAGGATTCAGAGGGTCTAATAGTACTTAACATTGGAAGCTA TATGGGTGGAGTAGATCTTTGGCAAAATGATTATGAACACGATGATAATTTTAGCATTCAGTGTATGCACGACAAGACTC TAGAGGTGGTATGTGTCCGTGGAGCGTGGCACCTTGGCAAACTACAGGTCGGTCTTTCTCAGGCTAGGAGGTTAGCACAG GGAAAGGTAATAAGAATACATGTTTCTAGTCCTTTTCCTGTTCAAATAGATGGAGAACCGTTTATCCAGCAACCTGGCTG CTTGGAGATAACTCACCACGGGCAGGTGTTTATGCTAAGAAGAGCATCGGATGAACCACGAGGGCATGCCGCAGCCATAA TGAATGAAGTGTTACTCGATGCAGAATGCAAAGGAGTCATAAATGCATCTCAAAAGAAAGTACTACTACAGCAAATGGCT CTCCATCTCTCGTAGTAAAAAAACAAAAACAGATCATCCTCTCACCAAATTTTGGTAACAAGAAGAGTTAACTTACAATC TTGTCTTACCCACTAGAAAGCCAGTCCCGGGTAC .......................................................................N........ .................i..................i..N........................................ ...........................N.................................................... .................N.......N.....N.....N.......................................... ..............N.......................................N........N................ .....................................N.........................N................ ........N.........i...N.....i.......N........................N.................. ...................i.................................N.............i.....i...... ............N...............................................N...........N....... ........i.....i..............N.................................................. N................................................................i......N....... ..........N..............................................N...................... ...........N.................................................................... ........N...................................................................N... ........................................N............N..........N............... ..................................................N............................. ..................N.........................................N.............i..... .....N...............................................................N..N....... .................N..............N..........N..i................................. .....................N.........................i..........N..............N...... ................N............................................................... ..........N..................................................................... ...................N.............................N.............................. ...............................i..................N..............N.............N ...N..............N......N................N...............................N..... ................................................................................ .................................. Pos Score Pred ------------------------ 72 0.177 - 98 0.582 Yes 117 0.547 Yes 120 0.400 - 188 0.456 - 242 0.759 Yes 302 0.391 - 338 0.188 - 346 0.255 - 352 0.405 - 358 0.299 - 415 0.398 - 455 0.491 - 464 0.375 - 518 0.351 - 544 0.406 - 569 0.229 - 579 0.709 Yes 583 0.341 - 589 0.735 Yes 597 0.249 - 622 0.259 - 660 0.702 Yes 694 0.352 - 708 0.510 Yes 714 0.839 Yes 733 0.135 - 781 0.188 - 793 0.392 - 802 0.677 Yes 811 0.519 Yes 859 0.347 - 889 0.556 Yes 895 0.649 Yes 910 0.235 - 961 0.292 - 1026 0.566 Yes 1033 0.370 - 1051 0.255 - 1098 0.252 - 1132 0.106 - 1209 0.205 - 1277 0.379 - 1321 0.139 - 1334 0.199 - 1345 0.201 - 1411 0.292 - 1459 0.313 - 1501 0.169 - 1515 0.566 Yes 1526 0.347 - 1590 0.261 - 1593 0.453 - 1618 0.154 - 1633 0.145 - 1644 0.374 - 1647 0.729 Yes 1702 0.223 - 1728 0.545 Yes 1739 0.221 - 1754 0.440 - 1777 0.165 - 1842 0.396 - 1867 0.338 - 1873 0.334 - 1882 0.180 - 1905 0.544 Yes 1931 0.420 - 2020 0.345 - 2050 0.289 - 2112 0.570 Yes 2131 0.347 - 2146 0.326 - 2160 0.481 - 2164 0.296 - 2179 0.172 - 2186 0.361 - 2203 0.411 - 2235 0.439 -

Explain the output -- Processing locus 186: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTATAGAGTATTTGGTGCAATGATAATGAAAAAGCATGTGCAAAGAAAAGGAAATCATAGCACTTCCTCATGTACTCC ACTCACTAGCGTTTTCTATAAAATGTCATGATTTGCGGTAGTGAAAGGCCAACACAAAGAATCATTTTACCTCTAAAGCC AAAGACCAACATGTCTAATTCAAACCAACCACTTCTTTCCAAACCAAAGTCTCTTAAGCACAAGAATCTATGCCTTGTCC TCTCCTTCGTAGCCATTCTTGGCTCTGTGGCTTTCTTCACAGCCCAATTAATCTCCGTTAACACCAACAATAATGATGAT TCCTTATTAACTACGAGCCAGATTTGCCATGGAGCTCACGACCAAGACTCATGCCAAGCTCTCTTGTCCGAATTCACGAC GTTGTCGCTCTCAAAGCTCAACCGCCTTGACCTATTGCACGTGTTTTTGAAGAACTCGGTGTGGCGGCTTGAGAGCACGA TGACCATGGTGAGCGAGGCTAGGATCCGCTCGAACGGTGTTAGAGACAAGGCAGGTTTTGCTGACTGCGAGGAGATGATG GACGTATCAAAGGATCGGATGATGAGTTCGATGGAGGAACTTCGCGGAGGAAACTATAATCTTGAGTCATACTCAAACGT TCATACTTGGCTGAGCAGTGTGCTTACAAACTACATGACATGTTTAGAAAGTATTAGTGATGTCTCCGTCAACTCTAAGC AAATAGTCAAGCCACAACTCGAGGACTTGGTTTCTAGGGCAAGAGTGGCTCTAGCCATCTTTGTCTCCGTCTTGCCTGCC AGAGACGATCTCAAAATGATCATTTCCAATCGCTTCCCGTCGTGGCTAACTGCTCTTGACAGAAAGCTTTTAGAATCTTC TCCCAAGACACTTAAGGTGACCGCGAACGTTGTGGTGGCAAAAGACGGCACCGGAAAGTTCAAAACAGTGAATGAGGCAG TGGCGGCAGCACCGGAGAACAGCAATACAAGATACGTTATATACGTAAAGAAAGGGGTTTACAAAGAAACTATTGACATA GGAAAGAAAAAGAAGAACTTAATGCTTGTTGGTGATGGCAAAGACGCAACGATCATAACCGGTAGTTTGAATGTCATTGA TGGATCCACCACCTTCAGATCAGCCACTGTTGCTGCCAATGGAGACGGGTTTATGGCACAAGACATATGGTTCCAAAACA CTGCAGGGCCGGCAAAGCACCAGGCTGTGGCTCTACGAGTGAGTGCTGATCAAACTGTTATAAATCGTTGTCGCATAGAT GCGTATCAAGACACGCTCTACACTCACACGTTGAGACAATTCTACCGCGACAGCTACATCACCGGTACCGTAGATTTCAT CTTCGGAAACTCTGCTGTGGTATTCCAGAACTGCGACATCGTGGCACGAAATCCTGGAGCTGGGCAAAAGAACATGTTAA CGGCTCAAGGACGGGAGGATCAGAACCAGAACACCGCCATTTCGATCCAAAAATGTAAGATAACGGCTAGTTCGGATCTT GCTCCTGTAAAAGGATCTGTGAAAACGTTCCTTGGTCGACCGTGGAAGTTGTACTCAAGAACAGTGATCATGCAGTCTTT CATTGACAACCACATTGACCCGGCTGGTTGGTTCCCATGGGATGGTGAGTTTGCGCTCTCCACATTGTATTATGGAGAGT ATGCAAATACCGGTCCTGGAGCGGATACGAGTAAGAGAGTGAATTGGAAGGGATTTAAAGTTATTAAAGACTCAAAAGAG GCCGAACAATTCACTGTGGCGAAGCTTATTCAAGGAGGATTATGGTTGAAACCCACTGGAGTTACTTTCCAAGAGTGGCT TTGATTTTAATGTCTACTTGTTCTTGTACTTTGTGTTGTGTTACCTCTGTTTTAGTTTGATGTATTTTTTCTGGTTTGTT TTGTATGACTTACCCAATAAATA .....................N.....N.........i.................................N........ ......................N....i.................................................... ..........i..........................................................N.......... ........................................................................i..i.... ............................N.....................i............................. ...............................................................................N .....i....................................................................N..N.. ..................N..N........N................................................. ..................................i....N...................N.................... ................................................................................ ...............i................................................................ .......................................................................N........ ................................................................................ .....................i............N...................................N........N ......................................N.............i.............N............. ..............................................................................N. ................................................................................ .........................................................................i...... ....................................................i........................... .....................................................................i.......... ....................................N....N.............................N........ N............................................................................... .........................................N...................................... .........N.................................................N.................... ....N.................. Pos Score Pred ------------------------ 22 0.332 - 28 0.388 - 38 0.701 Yes 72 0.279 - 103 0.224 - 108 0.599 Yes 171 0.602 Yes 230 0.414 - 313 0.561 Yes 316 0.507 Yes 349 0.429 - 371 0.666 Yes 480 0.380 - 486 0.693 Yes 555 0.419 - 558 0.467 - 579 0.259 - 582 0.386 - 591 0.212 - 675 0.582 Yes 680 0.255 - 700 0.450 - 816 0.743 Yes 952 0.146 - 1062 0.607 Yes 1075 0.221 - 1111 0.272 - 1120 0.307 - 1159 0.161 - 1173 0.769 Yes 1187 0.486 - 1279 0.209 - 1434 0.689 Yes 1493 0.725 Yes 1590 0.834 Yes 1637 0.404 - 1642 0.462 - 1672 0.447 - 1681 0.106 - 1802 0.291 - 1850 0.171 - 1900 0.038 - 1925 0.070 -

Explain the output -- Processing locus 187: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAGCAAGTGACTGGAAGGTCATACATTATTATTAGTTGGCTTATGTTTGATTTTTTCAAAAAAAACAAAATTAGCTGGT CGGCCGTTCAATTACCATTAGAATGAATTTATGAGTTCCTTAAGTATACATAAAGAACAGCTCTCTTTAGAAAGAGAAAA AACTGAAACTCAACTTATGCTTTGTTTCTTTTTTAATTTGAAAGCAATGTCTTCTACTGACCACGAAGAAGTTACCCTTC CGTTTCATCATCATCCTTTATCATGTGGTACTTTTGAGTCTTACACTTTCCGCTCCATATTATCTATCCCGAAGCTAAGA AATGGCATATACAACTGCTATCACTGCAAGGAGTGCAGCTTGACGTTTCACAAAGAATGGGCTGACAGCTTCTTCAGCCA GCAATTTATCTGCAATCACGTTCTCAAGATCTTCCGCCGGGCTAAGACACCATTAGTTGACAGTACAGATGACCACTTTT GTCATTTTTGTCGAAGCAAGCTTAAGTTAAAGCATTTCCGGTGCACGATATGCAACATAAGCGTGGGTAGAGAATGTGTG AAAGATCCACCACCACTCACAATTTATCAACCAAAGCACCACAAGCATAGCCTCACCCTCTTGTTAAGGTTAGTCACCTT TACTTGTAATGCATGTGGTGTGGAAGACGACCGCAATCCTTATGTTTGTCTTGAATGCAATTTGATGGTCCATAAATATT GTATTGAGAAACTACCACGCGTCATAAGCATAAACCGTCATGACCATCGCATAAATCATACCTTTCATCTTGGTCAAGGA GAAGGAGATAAAGAGTGTGGAGTTTGTCGGAAGAAGATTAATTGGGCCTACGGAGGTTATGAATGCTCTCGTTGTCCTAG TTATGCCGTTCATTCAAGGTGTGCAACAAGAAGAGAAGTGTGGGATGGGTTAGAGCTCGAAGATGTGCCCGAAGAAGAAG AAGAAATAGAAGATCCATTCAAGGTAGTTAATGAAAAGGGAGATATCGTTCATTTTTGTCATGAAGAGCATGTTCTCAGA TTGGATGAGAATTATGTTACAGATAATGTTAACATGCGTTGTCGGTGTTGTGTTCTTGCCATCGACGGGGATCCATGCTA CAGATGCCTGGAATGTGATTTCATACTTCATGAAAAGTGTGCTAATCTTCCTCGGAAGATGCGACATTTATTGCACAACC ATAAACTCTCTCTAGAGGTTTCATTCACACGTTTTCGTCGATCAGACAATTTCTTTTATACGTGTATTGCTTGTCAAACC CACACTGATGGCTTCATATACGCGTGTCATGATGGGCTTTGTAGATACCTCGTTAAAAGTATTAAGTATGATGTTCGGTG TAGCTCTGTATCAGAACCGTTTCACCATGATCTCCATCAACATCCCTTATACTTTACGTTGCAAAGTTCCAGACAATGTC AGGCTTGTGGCACAAAAATAGATCATGATCCCTTAAGTTGTACGGTTTGTGATGATTACTCTTTATGCATGAGGTGCACT ACTTTACCAAGAAAGGTAAAACACAGGTGTGATGATCATTTTTTGTCACTATGTCAAGGTTCTTCCGTAAACGATTCCGG CGACTTGTGGTGCGATATTTGTGAGACGAAGACAGATCCGAGTGTATGTTATTATACTTGTGATGAGTGTGGGCTTAGTC TTCATATTGATTGTGTACTTGGAGATTTATATTATTTGAAGCTAGGAATCCCAGAACCTGATGTTGAAATATGTCCAAAC AATGAGGCTACTCGACCTCTCTGCAGTGGTTGTAAAGTTCGTTGCAAGAACCCTTTCTTCATAAGGAGTACAGAAGAAGA ACCTGCATCACACGCTTGTTCAATAGACTGCTCATAAGGTCTAGTTGTTACTATTCGAATTCATGTTTTACATTCTCTGT CTAAATTGTAAAACAGTGTGCTTTAAAAGTCTTTCAACATTTTCTGTTAAGAACCA ...........................................N.................................... ......................N.......N................................................. ................i.............................N................................. ......................i......................................................... ....................................................................i........... .................................................N.......................i...... ................................................................................ ........i...i............................N............N.........i............... .......................................i........................................ ..........................................................N...N................. ..N.........................................N.................N................. ..............................N.............................N........N.......... ....N........N...........N.......i........................................N..... ...N........N................N............................N..................... ................................................................................ .......N....................N..N...................................i..N......... ..........................N................................................N.... ........................N..........................N............N...N........... ...............................N..................N............................. .............................................N................N................. ............................................................N.........i......... ..............................................................N................. ........................................................ Pos Score Pred ------------------------ 44 0.186 - 103 0.130 - 111 0.277 - 177 0.703 Yes 207 0.279 - 263 0.601 Yes 322 0.901 Yes 377 0.642 Yes 469 0.757 Yes 530 0.323 - 554 0.574 Yes 649 0.624 Yes 653 0.510 Yes 682 0.256 - 695 0.203 - 705 0.794 Yes 760 0.647 Yes 859 0.437 - 863 0.195 - 883 0.167 - 925 0.297 - 943 0.186 - 991 0.152 - 1021 0.126 - 1030 0.279 - 1045 0.131 - 1054 0.379 - 1066 0.316 - 1074 0.829 Yes 1115 0.264 - 1124 0.361 - 1133 0.131 - 1150 0.105 - 1179 0.309 - 1288 0.412 - 1309 0.399 - 1312 0.418 - 1348 0.592 Yes 1351 0.398 - 1387 0.393 - 1436 0.373 - 1465 0.336 - 1492 0.365 - 1505 0.156 - 1509 0.424 - 1552 0.264 - 1571 0.129 - 1646 0.126 - 1663 0.186 - 1741 0.354 - 1751 0.562 Yes 1762 0.371 - 1903 0.090 -

Explain the output -- Processing locus 188: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTACAATCACAACGCTAGTGGTCTCAGTTTCGAAGAGCTTTACAGGTACACTTCCATAGATTTCACTTTGATTCATCTC TTGTTGTTGTTGTTCTGCTAAACTTTGGTCGCTTATTTCAGAAACGCATACAACATGGTTCTACATAAGTATGGTGATAA GCTTTATACTGGACTTGTTACCACTATGACATTTCATCTCAAAGAGATATGTAAGTCTATTGAAGAAGCTCAAGGAGGAG CATTTTTAGAATTGCTTAATAGGAAATGGAATGATCATAACAAAGCGTTGCAAATGATTAGGGATATTCTCATGTATATG GATCGTACTTACGTTTCTACTACTAAGAAAACTCATGTTCATGAGCTTGGACTTCATCTCTGGAGAGATAATGTTGTGTA TTCGAGTAAGATTCAGACTAGGCTATTGAATACGCTTCTTGATTTAGTTCATAAGGAACGGACTGGTGAAGTTATAGATA GGGTGTTGATGAGGAATGTGATTAAGATGTTTATGGATTTAGGTGAATCTGTTTATCAAGATGATTTTGAGAAGCCGTTT TTGGAAGCTTCTGCTGAGTTTTATAAGGTTGAGTCAATGGAGTTTATTGAGTCTTGTGATTGTGGTGAGTATTTGAAGAA AGCTGAGAAGCCTTTAGTGGAAGAAGTCGAAAGGGTTGTGAATTATTTGGATGCTAAGAGCGAAGCGAAGATTACTAGTG TGGTTGAAAGAGAGATGATTGCTAACCATGTGCAGAGACTAGTTCATATGGAGAATTCAGGTTTGGTTAATATGCTTTTG AATGATAAGTATGAGGATATGGGTAGAATGTATAGTTTGTTCCGTAGGGTTGCTAATGGTCTTGTAACGGTTAGAGATGT TATGACTTTGCATCTTAGGGAAATGGGAAAACAGTTGGTTACTGATCCAGAGAAATCAAAGGATCCGGTTGAATTTGTGC AGCGTCTATTGGATGAGCGGGATAAGTATGACAGAATTATCAACATGGCATTTAACAACGATAAGACGTTCCAGAATGCG CTAAATTCTTCGTTTGAGTATTTCGTCAACTTGAACACACGTTCTCCTGAGTTTATCTCCCTGTTTGTTGATGATAAGCT ACGAAAAGGACTAAAAGGTGTGGGAGAGGAGGATGTCGATCTTATTCTTGATAAGGTGATGATGTTGTTTCGCTACTTAC AGGAGAAAGACGTTTTTGAGAAATATTACAAACAGCATCTGGCGAAAAGGCTTTTATCTGGAAAAACTGTGTCGGATGAT GCAGAGAGGAATCTGATAGTGAAACTGAAGACGGAATGTGGGTATCAATTCACTTCGAAACTTGAAGGTATGTTCACTGA CATGAAGACCTCACACGACACGCTGCTAGGATTTTATAATAGCCATCCCGAGCTTTCAGAAGGACCTACACTTGTTGTTC AGGTTCTCACAACCGGTTCTTGGCCCACACAGCCAACCATACAATGTAACCTACCTGCAGAAGTTTCTGTTCTGTGTGAG AAGTTCAGGTCATATTACCTCGGGACTCACACCGGTAGGAGATTGTCTTGGCAAACGAATATGGGAACAGCAGATATCAA AGCAGTGTTTGGAAAGGGTCAGAAGCATGAACTAAACGTTTCGACTTTCCAAATGTGTGTCCTTATGTTGTTCAACAACT CTGATCGACTAAGCTACAAAGAGATCGAACAGGCAACTGAAATCCCCACCCCAGACCTAAAGCGTTGCTTGCAGTCAATG GCGTGTGTAAAAGGTAAAAACGTGCTAAGAAAAGAACCAATGAGCAAGGAGATAGCAGAGGAGGACTGGTTTGTTGTGAA CGACAGGTTCGCAAGCAAGTTCTACAAAGTGAAGATAGGAACTGTGGTGGCACAAAAGGAGACAGAACCAGAGAAGCAAG AGACAAGACAGAGAGTAGAAGAAGACAGAAAACCTCAGATCGAAGCAGCCATCGTGAGGATAATGAAGTCTAGACGAGTG TTGGATCACAACAACATAATCGCAGAGGTCACCAAACAGTTGCAGACGCGGTTCTTGGCAAACCCAACAGAGATAAAGAA GAGAATTGAATCACTCATTGAGCGTGATTTCTTGGAGAGGGATAATACAGACCGGAAACTTTACCGCTATCTAGCGTAAA AAAGTCTGGATTGATTACACGGTCCCTCTGTTTATTTCGCATCGTTTCTTCTGTTAGTCAGCATTTCTTATTTGTTCTGT AGTCTGGTAAGTTATAAA ................................................................................ ......................................................i...............N......... .........................N......................N............................... .........................N....N......................i.................N.....N.. ..................................N.....N.............................N......... ................................................................................ ........N......N..........N.....N...........................N................... ....................................i........................................... ..................................................N............................. ..............N............N...................N.......................N........ ............N..............N................N..............................N.... ......................................................................N......... ................................N.........................N..N.................. ...........................................................................N..N. ...................................N.................................N.......... ...........................................N.................................... ............................................................i................... ..........................N.........................i...........N............... .............................................................................i.. .......................................i........................................ ................................................................................ ..............................................................N................. ................................................................................ ................................................................................ ................................................................................ .................. Pos Score Pred ------------------------ 135 0.589 Yes 151 0.267 - 186 0.292 - 209 0.477 - 266 0.387 - 271 0.176 - 294 0.513 Yes 312 0.466 - 318 0.406 - 355 0.163 - 361 0.063 - 391 0.137 - 489 0.357 - 496 0.074 - 507 0.299 - 513 0.246 - 541 0.097 - 597 0.698 Yes 691 0.052 - 735 0.382 - 748 0.230 - 768 0.382 - 792 0.462 - 802 0.136 - 811 0.219 - 819 0.357 - 828 0.834 Yes 856 0.119 - 877 0.140 - 882 0.592 Yes 903 0.526 Yes 973 0.104 - 988 0.186 - 1005 0.255 - 1036 0.230 - 1111 0.120 - 1153 0.232 - 1179 0.447 - 1182 0.449 - 1276 0.114 - 1279 0.226 - 1316 0.298 - 1350 0.481 - 1362 0.379 - 1484 0.475 - 1581 0.776 Yes 1627 0.226 - 1653 0.665 Yes 1665 0.377 - 1758 0.807 Yes 1800 0.666 Yes 1983 0.310 -

Explain the output -- Processing locus 189: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATTTGAAAGCACTAATCAATCTTTAGGTTTTTTTATTTTGAAACTCGACGAACTTTTTTTATTTTGTCAATTTCTGGA TCGAGTTTTTGGTTTCTCTGACAAGTTACCGATACGATATTTGATATGGCCGATTCTAGGAAGCGAGATACCGGAGACGA TCATCAGCACCGTGATGATCAACCAAATGACGGTGATGATTTGTCTATTTCGTCGTCGACGACTGATGACTCTCAATTTA ATGGAACCGAAGGAGAGAATGAATTGACGAGGATTGAGAGTAGAGTTTCAGATCCGTTGACTGATGCAACCGGAGGAGAC TTTTTGGTTGGAGAGGATAGGGTTTTACGGTGGTTACAAGCTTTGGACATGCAAGTTATGGGAGCTTGTCGAGGTGATGA GAGATTGAAGCCGTTGTTGAAGCTTAACGTTTCTAATGGAATGGCTGAAGATCGATTGCTTGCGCATCTTAGCCAGCACT TCGAGCCTGCGGAAATTGGTATGCTGGCTAGATGTTTTTGCATACCTCTGGTTTCGGTTCGCGTCGGGAAGATTATAAAG GAAGGGATTCTAATGCGCCCCACTCCCATAAGGGGTAACCTGAGCCTTATGGTCTTGCCAACATCTGACTTGAGACTCTC ATTTATTGGAGACAATGGTCATTCAGAACAACTCTTTACCTATACCAGCAAATCTCAGTGCTCTGCGGTGTCAATTGAGG AGATAACTGTGGACAGCTCTGGTAGATCTTTTGTTATAAGGATCGCAAATGGCAATGCTTTTTACTATTGGTGCTCAGAG AAGTCAAAACTTCTGGGAACTGAATTGCGTAGGAAGATGAAAGACTTGATCAAGAAGAAGCCATCAATCTCAGAATTAAC TGGAATTGAAGAATCACGTCTTGGCTCTGTTGCTTCTCATCTCCGTTTGTATCTCATGGGTTCAGTGGTGCCTAACATCA AAGGTTGTCAGGTCCCATCTCCTGATTCATCATCATCTTCTGGCTTTTCTGAAACAGCTGATTCCTCCTCGAGTGCATCA TCAAAATCTCTTAGAGCACGCCACTGTGGGACTCAACAGACAAAGACCCAAGGAAGCCTCAGCCCTAGAGCAAGCTCCTT CAAAGAAAACACACTGAGAAATGCTTCTCTACGTATCTCCTCTAGAGATAAATCAAAGGGACACAGTGAAGGCCATTTCT CAATCTTTGATAATTCATCCATTACCTCTATCCCCACCAATGTAGAGGGTTTTATCCAGTCAGAAGGAGAAGTTGAAGAA GCTACTGAGAATTATAATGGAATCAGACAAATTATTGCTTTTGAAGAAGCAGAGTCAACACCAAGTACAATGACCGGACC TCCCCCATTTCCTTTGAAGATGGGTCCACCAGTCTTTTCTCCTTATTATTGCTGGTGCCCTCCAACCACTTCATCGCTTC ACGCACCATCTGCCTCTTATCAGTTCCCTCCACTATCCATTGAGCTACCTTCCCTTCCGCCACTTTCCTCCTTATTACCA GCATCGGGATCAGATGGATTCTTGATCCCGTCATCACCGCTCGATCTCTCTGACATCCCTCCATTACCATTAGTCCACCA CATACCCATTCCAGGCTCATCATCATCATCATCCCAGCAACAGATGATGATTCCTATAATGTGCGACCCTATTGTTCATA TTCCAGTTATCGATATCTTCTCTTCAGGTCAAAGCTATCTGGTAAGCGCTGGACCCACCGGGATAATATCCACGGGCATC CCTCCGCTCCCAGTGGAGAATGATTCGCTGGTCGAGAAAGGTGCAAGAGAAACACTACGATTGCTCATAAGCGGAGCCAA TGCAACAACATCTACACCTTTGAACCATCATGGAAGCAGAGGTCTATACAGTGTGAGCCGGGATGTGAGTGGTGTGAGCT TGTTTGCACCTATTGGGTTGCAACAACCGAGTTCAGTTGAGGGAGGAGATGGTGGTGGTGAGAGTGTGAGCTCAAGCGAA GCAGTGCCTGCGCCTCCCAGAGAGACTTCTGGTTAATTTTTTTGGTTCTTTGTAAGTGTGGTCCGAGTTAAGTGTATAGA AAACTCATCTCTCGCCTAGTTTTTGGTTTTGTTCAGCCCAAGTCTTTTGGTTTCA ................................................................................ .............................................i.................................. ..............N...........N........N.............................N.............. N.................N............................................N................ ................................................N........N..................N... ...................................N....i....................................... ....................i..........i................................................ ............i...................................N............................... ..............N................................................................. ................................................N.....N......................... ....................................N........................................... .......................................................N........................ ................................................................................ ................................................................................ ....................N........................................................... .......................................i........................................ ................N....................................................i.......... ...................i............................................................ ................................................................................ .............i.................................................................. ...........................................i..N...........i..................... ................................................................................ ...................N...........................................................N .............................N................................i................. ................................................N............................... ................................................................................ ....................................................... Pos Score Pred ------------------------ 126 0.751 Yes 175 0.116 - 187 0.147 - 196 0.151 - 226 0.180 - 241 0.253 - 259 0.237 - 304 0.325 - 369 0.239 - 378 0.491 - 397 0.180 - 436 0.162 - 441 0.533 Yes 501 0.719 Yes 512 0.695 Yes 573 0.550 Yes 609 0.380 - 655 0.410 - 769 0.392 - 775 0.192 - 837 0.261 - 936 0.482 - 1141 0.497 - 1240 0.565 Yes 1297 0.164 - 1350 0.546 Yes 1380 0.522 Yes 1534 0.780 Yes 1644 0.659 Yes 1647 0.475 - 1659 0.811 Yes 1780 0.279 - 1840 0.238 - 1870 0.422 - 1903 0.545 Yes 1969 0.351 -

Explain the output -- Processing locus 190: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTTAGCATGAACATTGAACCAAAACCAAACCGAAATTGATTCCGGTTCGGATTTGATTTGACTACAATAACCAAGGCT ATATATTTAACTAAAATATGAATTATTATAGTTGCAAACAAGATGATGATGATGATGTTATACGAGCGAGAGACTCAACG GTTATGGAATCCGACTGAGAGAAAATGCTTTAATCTTCTTCAACAATGTCACCGAAACGGAAGATTCTCTCTTCTTCAAA TTCATGCTTTCATGCTCCGCCACGCCATTGAGACGAACGTTCAGATCTTCACCAAATTCTTGGTTATTTCTGCTTCGGCT GTTGGAATTGGTTATGCACGTAAGTTGTTCGATCAAAGGCCTCAGAGAGACGACAGTTTCCTCAGTAACTCTATGATCAA GGCCTATCTTGAAACCCGTCAGTATCCTGATTCGTTTGCTCTTTACAGAGATCTTAGGAAGGAGACTTGTTTTGCTCCTG ACAATTTTACTTTCACGACTTTGACAAAGTCATGCTCTTTGAGTATGTGTGTTTACCAAGGTCTGCAGTTGCATAGCCAG ATTTGGAGATTTGGTTTTTGTGCAGATATGTATGTTTCGACTGGAGTTGTTGATATGTATGCTAAATTTGGGAAGATGGG ATGTGCGAGGAATGCGTTCGATGAAATGCCTCACAGAAGTGAGGTTTCTTGGACGGCTCTAATCTCTGGGTATATTAGGT GTGGGGAGCTGGATTTGGCGAGTAAGCTCTTTGATCAGATGCCTCATGTTAAGGACGTTGTGATATATAACGCAATGATG GATGGTTTTGTTAAATCTGGTGATATGACCTCTGCTAGAAGATTGTTTGATGAGATGACTCATAAAACTGTCATCACTTG GACCACTATGATTCACGGTTACTGCAACATTAAGGACATAGACGCCGCTAGGAAGTTGTTTGATGCTATGCCAGAGAGGA ACCTAGTTTCTTGGAACACAATGATTGGGGGATACTGTCAGAATAAGCAACCTCAAGAAGGTATAAGGTTGTTTCAGGAA ATGCAGGCAACCACCTCGCTAGATCCGGATGATGTGACTATTTTGAGTGTGCTACCAGCTATTTCTGATACTGGTGCTCT CAGTCTCGGTGAATGGTGTCACTGTTTTGTGCAGAGGAAAAAGCTCGATAAGAAGGTGAAAGTGTGTACAGCAATTCTTG ATATGTATTCGAAATGCGGGGAGATAGAGAAGGCGAAAAGAATCTTTGATGAGATGCCAGAGAAACAAGTAGCGTCTTGG AATGCTATGATACATGGGTATGCTTTGAATGGGAATGCACGTGCAGCTTTGGATCTATTCGTAACAATGATGATAGAAGA AAAGCCAGATGAGATCACAATGCTCGCGGTTATCACAGCTTGTAACCATGGTGGATTAGTGGAGGAAGGAAGAAAATGGT TCCACGTGATGAGAGAAATGGGCCTGAATGCTAAAATTGAGCACTATGGCTGTATGGTTGATCTACTAGGCAGAGCAGGG AGCCTAAAAGAAGCAGAGGATTTGATTACCAACATGCCTTTTGAGCCAAATGGTATTATTCTAAGCTCTTTCTTGTCTGC GTGTGGTCAATACAAAGATATAGAAAGGGCTGAGAGAATTCTGAAGAAGGCAGTCGAATTGGAACCTCAGAACGATGGGA ATTATGTGCTGTTGAGGAACTTGTACGCTGCAGATAAAAGATGGGACGATTTCGGGATGGTGAAAAACGTGATGAGGAAG AACCAAGCCAAGAAAGAGGTTGGATGTAGCTTGATTGAGATAAATTATATCGTTTCGGAGTTTATATCAGGAGATACAAC CCATCCTCATCGGCGAAGCATACATTTGGTTCTCGGAGACCTGCTTATGCACATGAATGAGGAGAAATACAACTGGTGAA AGCAACTCACAGTTTCATGTTAGGCCATCAAATGCTTCTACTCATTATGAAATACTTGGGGCGATAAGAGTTAGTAGTGG CATTCTAAGAGATCTCAT ........N....................................................................... .................N........................N..N..N..N..N......................... ...N....................N....................i.................................. ...N.......N.................................................................... .............N..........................................................N....... ................................................................................ ...............................N............i................................... ...........................N...N......................i...N................i.... i..........N........N....N...................................................... ......................................N......N............................i..i.. .......i......................................................N....N............ ....................i........................................................... i...........................N..N................................................ ............i................................................................... ..i..........N..................................N....N.......................... ........N..........i...........................N...........................N.... ........i........i.........N.................N.......N.......................... .................................i...............N.............................. ..........................................................................N..... ...N....................................i...............i..............N........ .......................N........................................................ ..............................................N.....N...N....................... ................N..............N..............N................................. .................. Pos Score Pred ------------------------ 9 0.210 - 98 0.477 - 123 0.271 - 126 0.250 - 129 0.196 - 132 0.237 - 135 0.109 - 164 0.231 - 185 0.232 - 206 0.547 Yes 244 0.093 - 252 0.318 - 334 0.231 - 393 0.301 - 512 0.350 - 525 0.649 Yes 588 0.468 - 592 0.121 - 615 0.629 Yes 619 0.095 - 636 0.764 Yes 641 0.714 Yes 652 0.201 - 661 0.217 - 666 0.424 - 759 0.312 - 766 0.068 - 795 0.704 Yes 798 0.669 Yes 802 0.230 - 825 0.805 Yes 850 0.131 - 855 0.742 Yes 888 0.611 Yes 943 0.189 - 948 0.370 - 981 0.571 Yes 1041 0.610 Yes 1069 0.211 - 1072 0.138 - 1133 0.560 Yes 1203 0.587 Yes 1214 0.436 - 1249 0.108 - 1254 0.458 - 1282 0.223 - 1287 0.398 - 1294 0.109 - 1300 0.100 - 1309 0.141 - 1315 0.227 - 1347 0.511 Yes 1350 0.522 Yes 1369 0.351 - 1380 0.582 Yes 1408 0.428 - 1436 0.484 - 1449 0.589 Yes 1458 0.681 Yes 1468 0.344 - 1486 0.242 - 1494 0.353 - 1554 0.676 Yes 1570 0.132 - 1675 0.408 - 1684 0.264 - 1721 0.533 Yes 1737 0.627 Yes 1752 0.466 - 1784 0.260 - 1887 0.331 - 1893 0.182 - 1897 0.340 - 1937 0.069 - 1952 0.052 - 1967 0.056 -

Explain the output -- Processing locus 191: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAGTAATTACAAAAGATATGATTGAGTTACCTAATATGAGTAAGGGTAATTATAGAAGAGATATTATTATTAGACGAGA TTGATTTAAACTTAGCTATAGCAAGAGAATATATACTTTAACAAAGAATATATAAAAGGAGAAATGGTGCCAGCTAATTT AAGTCGAAGGATCACAACTTTTCTCTTGGGTCAAAGCTGTCATTGCTCTCTCTCTCTCTTTCTCTCATATATACTGCTCT GTATCTCTAAAAAACAACACTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTTCTCAAATCTTGGAGGAGAAGAAGCAAA GAATTAGACTACTCTGCAACAATGGCTATTTATTCTGTCCATAACGGCGACAAGAAACATTGGTGGTTCACTCACAAGAA GATTGTTGATAAGTACATTAAGGACGCAAGATCTTTAATGGAGAGTGAGGAGCAAAACGACGTTGCTTCAGCCATTCATT TACTAGACGCGGCTTTATCAATATCCCCGCGTTCGGAAACTGCGTTAGAACTTAAAGCCAGATCTTTGCTCTTTCTACGT CGCTTCAAAGATGTAGTTGATATGCTTCAAGATTATATCCCAAGCCTCAAGCTTGCCGTGAACGAGGAAGATGGATCTTA TTCATACGAAGGTTCTTCTTACTCTTCCTCCTCTTCTCAGCTCTCCCGGAAACTTCTCTCTGACTCATCTCCTCGCCGTG ACTCATCCTTCAAGTGCTTCTCTGTTTCTTACCTGAAAAAGAAAATTATGGCTGGGATTTGTAAAAATCGCGATCAAGAT AAACAATGGAGATACGTTGTTTTAGGACAAGCTTGTTGCCACCTAGGATTAATGGAGGACGCATTGGTTCTTCTTCAAAC CGGAAAACGTCTCGCAACAGTCGAGTTCCGCCGTCTAAGTGTAAGCTTGTCAGATGATAGCGTCTCTCTCCTCCTCTCTG AATCGTCATCATCATCATCCTCATCCTCTTATGCTTTTCCTCCACGCAAAGTCTCCGAATGCGAAACCGTCACTAACCTT CTCGCCCACACGAAAAATCTCCTCCGGCGTCGCTCCGCCGGATTCGCCGCCTTTGACGCTGGACTTTTCGCCGACTCGAT ACGTCACTTCTCCAAAATTCTCGACGGTCGTCGTCGTCCCGCGCCACAAGGATTCCTCGCTGATTGCTATATGCACCGCG CCGCCGCTTACAAATCCGCCGGGAAAATCGCGGAAGCGATCGCCGATTGTAATAAAACCTTAGCTCTTGAGCCGTCGTGT ATCCATGCGTTGGAGACTAGAGCCACTCTTTTGGAAACGGTTCGGTGTCTACCGGATTCGCTTCACGATTTAGAGCATTT GAAGATACTCTACAACACTATCTTACGTGATCGGAAACTTCCCGGACCACCGTGGAAACGACACAATGTGAAATACAGAG AGATACCGGGAAAGTTATGCGAACTGACGACGAAATCGAAGAAACTGAAAGCAAAAATGGCGAATGGAGAAATCGGAAAC GTTGATTATTACGGATTGGTCGGAGTTAGACGCGGTTGCACGAGATCGGAGCTTGATCGAGCAAATCTCTTGCTCTGTCT CAGGCATAAACCGGACAAGGCTTTAGCATTCATGGAACGTTGCGATTTCTTCGATCAGAGTGAGATTAGTTCCGTTAAAG ATCGAGCGAAGATGTCTTCTTTGTTGCTTTACCGATTGATTCAGAGAGGTTATACAGCTTTGGCGGCAGCGATAGCTGAG GAGGAACAGAGGAAGAAGATGATGGTGTTGACTCAAATGTCAACGAAAACGGTTGAAGAACATGAACCGGTTGAAAAATC CGGTTCAATTACTTTAACCGATTTTGCAGAAATTAAACCGGGGAATTCGAACGCGTACCAAGGAGTTTTCTGCCGAGATC TTGCTGCTGTTGGGAGTTTGTTATCTAGGACCGGATTTAACCAACCGATACCAATGAAATACGATGCGATCAGTTGTTAA CCGGGAATTTTAGTTTGTATATAATTAAGAATTTTTTGTCACTTTAAAATATTCGTCTCTTTTGAGTTAATTCTCCTACT TTCTCTTTATTTCTGTGCA ..................N.................N........................................... ...............................................................i................ ................................................................................ ................................................................................ .....................i.......................................................... .....................................i.......................................... ................................................................................ ..........N..........N................................................i......... ................................................................................ ...............................................i................................ .....i.............................................i............................ .....................................................i.......................... ..............................i...........................N..................... ................................................................................ ......................................................................i......... ................................................................................ ....N........................................................................... .................................................................N.............. ................N.......................................i......i................ ................................................................................ ...............................N................................................ ...........i.................................................................... ..................i..i..............i........................N.................. ................................................................................ .....................................................N.........N................ ................................................................................ ................... Pos Score Pred ------------------------ 19 0.384 - 37 0.350 - 144 0.501 Yes 342 0.844 Yes 438 0.764 Yes 571 0.483 - 582 0.310 - 631 0.639 Yes 768 0.852 Yes 806 0.790 Yes 852 0.631 Yes 934 0.544 Yes 991 0.679 Yes 1019 0.302 - 1191 0.556 Yes 1285 0.151 - 1426 0.482 - 1457 0.316 - 1497 0.602 Yes 1504 0.518 Yes 1632 0.425 - 1692 0.539 Yes 1779 0.727 Yes 1782 0.655 Yes 1797 0.561 Yes 1822 0.398 - 1974 0.123 - 1984 0.296 -

Explain the output -- Processing locus 192: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCAGAAATTCCTTTTTTTAACCTAGTTCTAGTTAAAATAGTAAAAGGAATTTTGAACCGAACGTTAAAAAAAGAAGTC TCGTAACTCTCTCAATTATGAGACGGCGAAATGAAACCCCCAAAGACGTTAACGACGACGACGGCGGAGGAGGAAGAAAC AACCATGCGGAAACAGCCGCCTTCGCCTCCAAAGCCACCACGTGTCAATTCTATGCTCCAATGGTGGCGGAGATGGACGG TGTTGGTGGCCGCTATCTGGATTCAAGCATTCACCGGCACGAACTTCGATTTCTCTGCCTACTCTTCCGACATGAAGTCT TCCATGGGGGTTTCTCAATCTCGGCTTAACTACATGGCGGTTGCTTCAGATTTGGGTAAAGCATTGGGTTGGTCTTCTGG TTTCGCCATTGCCTATTTCCCTGTCTCCGGCGTGCTTTTCGCTGCCGCCGCAATGGGACTCGTCGGTTACGGTGTCCAGT GGTTATCCATCGCCGATGTGATCGATCTACCTTACTCTCTGGTTCTGGTTTGCTGCTCCTTAGCCGGGTTAAGCATCTGC TGGTTCAACACCGTCAGCTTCATCCTCTGCATTCGACATTTCAAAGCGAACCACTCGTTGGCCCTGTCTCTCGTTGTGAG TTTCAACGGAATCAGTGCCGCGTTGTACACGCTTGGCCATGAAGCCATTAGTGGGAAATCATCGGCAAGCTCTGATATTT ACCTTCTCCTTAACTCTCTCATCCCTCTGATCGTCTCTGTTTTAGCCCTTTGGCCGGTTCTTACTAACCCTAATTCCTCT GAAACCGATACTACGCGAACGCATGACGAAACCAGAATCTTCGTAGTCTTCAATGTCTTAGCCTTAGTTACATGCTTTTA CCTTCTCTTGCCTTCCTCAGGCACCTACTTAGCCTCATCACCTCGTTGGCATTTCCTCGGAGCTATTTTCCTTCTCCTTT TCCCATTGTGCGTCCCGTTTCTCGACTATATTCACCGTGCTCTCGAGTCCTGTTTCCACCACCACAGCTCTGGCTATGCA GCGGTTAACATTGAGGAGCCCAAGATCCTCAAAATCAAAAGTCAGAAGAGTAATGCGGAGGAGGAAAGTGATCAAGTGAG ACTAGGAGATGAACATTCTCTCGGAATGCTTGTTCGTAAGCTTGAGTTTTGGTTATACTACGTAGCTTATTTCTGCGGCG GCACGATCGGTCTTGTTTATAGCAACAACTTAGGACAGATCGCTCAGTCTCTAGGACAAAGCAGCTCAAACGCTAAATCC TTAGTTACACTCTTCTCTGCCTTCTCCTTTCTCGGAAGATTGCTCTCCTCTGCACCTGACTTCACACGCAAGAAGCTAAA ATACTTGACAAGAACAGGCTGGTTCACGATTTCGCTACTGCCAACACCTTTGGCATTCTTCATCCTCGCGTATTCACCCA AGACCAACCAGACCGCGCTACTAGAAGTTGCGACAGCACTTATCGGGTTAAGCTCGGGGTTTGTGTTTGCGGCTGCAGTT TCCATAACTTCCGAACTCTTTGGACGCAACAGTGTCGGTGTCAACCAGAACATCCTCATCACAAACATTCCCATAGGATC TCTCTTCTACGGCTACATGGCTGGGTCTGTCTATGACACAAATGCCAGCCTCGGCAGGAAATCTGTGGTGGCTGACTCGG TTGTTTGCGTAGGAAGAAAGTGTTATTTCGTAACGTTTTTGTTTTGGGGTTGTTTGTCTGTTTTGGGGTTTGTATGTAGC TTGTTTCTGTTTATTAGAACCAGACCGGTTTATCACCGGTTAAAATTGAACAGAATGTAAAACTGTGTAGAGTATTATCA GCTGCCTGCTTATAATGGTATAGATTGGTAGGATCCAAGAAAAGAGTGACCATTGCCTTCACACGACTCAGCCAATTAT ................................................................................ .................i............i................................................. ....N...............................................N.......N...........N....... .......................................................................i........ ...i.............................i.............................................. ....................................................i........................... ...............N................................................................ ................................................................................ ......................................N......................................... ................................................................................ ......................N.............................i..................N........ ................................................................................ ...........................................................................N.... ....................................................N........................... ........N................i...................................................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................i...............N........N...................................... .........................................................................N...... ......................................................N......................... ..............N................................................................ Pos Score Pred ------------------------ 98 0.726 Yes 111 0.638 Yes 165 0.285 - 213 0.098 - 221 0.470 - 233 0.436 - 312 0.692 Yes 324 0.832 Yes 354 0.734 Yes 453 0.551 Yes 496 0.402 - 679 0.094 - 823 0.382 - 853 0.530 Yes 872 0.231 - 1036 0.371 - 1093 0.343 - 1129 0.184 - 1146 0.539 Yes 1617 0.621 Yes 1633 0.202 - 1642 0.221 - 1754 0.108 - 1815 0.387 - 1855 0.110 -

Explain the output -- Processing locus 193: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGGACAGTCACAAAACTCCAATCTCTCTCTCTCCTCTCCGTCTCTGCGCGCATGATCCCCCCCATAGTGTTCCACTAC GCACCGCCCGTGACTCTCTAATGGTCTGTGACTTGTAATCCATCTCTCCTTATTTTTATTTCAATTTTATTTCTTTCCAA AATTTTAATTAATTTCCACACACACACACACCTATACATATATATACACACACATTCGTCTCTAATGGCTTCTTCGAAAC TCCATCATCATCCTCACCGCAGAAAATCCCCAATACGTTGCTTCTGTCTCTTAATCATAACCATGTCCTCCATTCTCATC CTTCTCTTCTCTCTCTTCCTCTTCTCCTCTCCCTCCTCCTCCTCCCGTCACCACCACCACCACAATTCCGGCGACACTTC TCCGGTCAATCCTTCGTCGTCTCTCGCCGCTCAGATCCGTCTCGCTTGTAACGCAACTCGTTACCCAGACCAATGCGTCT CTTCCCTATCCGAACAGGGTCGGGTTCCTCCGGATCCTAAACCAATCCAGATCATTCACTCAGCGATCTCATTCTCATTC CAAAACCTCAAAACCGCCCAATCAAAAATCAAGTCTATTGTAGACTCGTCCGTAGGGAATCTAAACCGTACAAACGCCGC GAACACGTGTCTCCAGCTTCTCACTTACTCCGAGCACCGCACTCAATCGACGGATCAAGCTTTAACACGTGGCAAAATCA AAGACGCTCGAGCTTGGATGAGCGCTGCTCTAGTGTACCAGTACGATTCATGGTCAGCGCTCAAGTACGTCAACGACACG AGCCAAGTAGGTGAAACGATGTCGTTTCTCGACGGACTTATCCACGTCACCAGCAACGCGCTTAGCATGATGGTATCGTA CGATAACTTCGGAGACAACGTCGCGTCGTGGACTTACCCAGCAACGGAACGTGACGGGTTTTGGGAAAAGACTGGGCCTG GTTTGGGCTTAGATCCGAGTACAGGGCTTAATTTGGGTTTTCCTTCCGGTTTAAAAGAAGACGTGACGGTGTGTAAAGAC GGAAAATGCGGTTACAAGACGGTGCAAGACGCCGTTAACGCTGCGCCGGAGGATAACGGAATGCGTAAGTTCGTTATAAA GATTAGCGAAGGAGTCTACGAGGAGAACGTTATTGTTCCGTTTGAGAAGAAGAACGTTGTGTTTATCGGTGACGGTATGG GCAAAACGGTCATTACAGGTTCGTTAAACGCCGGTATGCCTGGGATCACTACGTACAATACTGCAACTGTCGGAGTGGTA GGAGATGGATTCATGGCTCGTGATCTAACGTTCCAGAACACGGCGGGACCAGACGCTCACCAAGCGGTGGCGTTTAGATC CGACAGCGATTTTTCTCTGATCGAGAATTGCGAGTTCCTTGGGAACCAAGACACTCTCTATGCCCATGGTCTTCGTCAGT TCTACAAAAACTGTCGTATCCAAGGAAATGTTGACTTTATCTTTGGGAATTCAGCTGCTGTCTTCCAAGACTGTGAAATC CTAATCGCACCGCGTCAAATTAACCCTGAAAAGGGCGAGAAAAACGCAGTCACTGCCCAAGGAAGGATTGACCCGTCCCA GTCCACGGGTTTCGTGTTCTTGAACTGTTTGATTAACGGAACAGAGGAGTACATGAAGCTGTTTAAAGCTAACCCTAAGG TGCATAAGAACTTCTTGGGGAGGCCATGGAAAGATTACTCAAGGACTGTGTTTATAGGTTGTAATTTAGAGGCTTTGATT ACTCCTGATGGATGGTTACCTTGGAGCGGGGATTTCGCGCTCAAGACACTTTATTACGGTGAATCTAAGAACACGGGTCC GGGATCAGATAGGTCACAAAGGGTTTCATGGAGCAGTCAGATACCGGACGAGCATGTTCATGTGTATTCGGTGGCTAACT TTATTCAGGCTGATGAGTGGGCTTCGATGTCTGCTTAAATCAATCTTTAAGACTATAAGGGGTTTAGGAATGTTTTAAAA GAAGAAAACCATCTCATTGTAATTTAGTTGATAAACTGTTTTAAACATAAAAGCAAA .....................................................N.......................... ....................N........................................................... ................................................................N............... ..............................................................i................. ................................................................................ ........................................................................i....... ................................................................................ ................................................................................ ................................................................................ .................N...............................i.............................. ..................i...............................................i..i.......... ................................................................................ ................................................................................ .....i......................................................i................... ............................................................................i... ...................................i............................................ ....N.......i................................................................... ...........................................................N.....N.............. ...........................i.................................................... ................................................................................ ....................................................N........................... .........................N...................................................... .......N...i.................................................................... ...........................N.........................N.....N.................... ............N.............N..........................................N.......... ......................................................... Pos Score Pred ------------------------ 54 0.153 - 101 0.349 - 225 0.427 - 303 0.735 Yes 473 0.601 Yes 738 0.259 - 770 0.682 Yes 819 0.874 Yes 867 0.626 Yes 870 0.508 Yes 1046 0.631 Yes 1101 0.744 Yes 1197 0.644 Yes 1236 0.607 Yes 1285 0.250 - 1293 0.830 Yes 1420 0.365 - 1426 0.212 - 1468 0.515 Yes 1653 0.390 - 1706 0.313 - 1768 0.263 - 1772 0.656 Yes 1868 0.152 - 1894 0.160 - 1900 0.118 - 1933 0.187 - 1947 0.402 - 1990 0.089 -

Explain the output -- Processing locus 194: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTCATTTGAGAGAAAGATATAATTTGTGATTGTTGAAACTTGAAACTTGAAGTCTTGAAGAAACAGAAAGAAAGACTTG AAGTCATGGTCTCATTCCTAACGCCCAAAACAACAAAGATTAATAATGTGCAAAAGATGTATTGGGCCTAAAAGGCCTGG CCCATTAGCTGGATTCCGTAAGAAAAAAAAAAAAAGTGAAAAGGAAAAGAAGAAATCGTCGTTGTTGTGGAGAAGGAGAA GAAGGATAAGGATGATAACGGTAAGCGAAGTTTCCTCTTACTCTTCTTCTTCTTCAAACTTCGCGTCTCTGTCTCGTCTC AATCACAAATCATCTTCGAGATTGCGAAGCTCTTCTCTTTACAAAGGAAGCTTCTTCTCTGTTTCGACAAAGACCAGGAG GAACACTTGTAAAGCCAAATCATGGAATCTCGGTCTTGTTATCAACTCTAGAAGCTCTGAGGCTTCGGTTTTTGATCCGT TGGGGATCAATCCAGATGAAACTTCTGGTTTAAGTAGCATTTGGGAAAGCTTTGTGAGTCTTTTGTCACCATCATTTGAA AGCTCTTCCGGTAACAGGCGAGATAAGCCGTCTTCTGGTAGAGGCGTGGCAGCTGCTATTGAGGACAGTTCCATTGATTT TGGAGATTTCTTCAAAGGGCCATTGCCAGGGAAGTTTCTGAAGCTTCTTGGGTTTTTAGCTCTCTCTCGGCTTGGAATTT ACATACCACTCGGCGGGGTTAATCGAGAAGCTTTTGTTGGAAATTTGGATCAGAATAGCATTTTGAGCACTTTGGATACA TTTTCTGGTGGAGGCATAGGTAGACTTGGTATATGTTCTCTCGGGATTGTTCCTTTCATCAACGCTCAGATTGTGTTTCA GCTTCTTGCTCAAGTCTATCCAAAGTTGCAAGATCTTCAGAAAAAAGAAGGTGAAGCTGGTAGAAAAAAGATTCTTCAGT ATACTAGATATGCTTCAGTTGGTTTTGCGATAGTACAAGCAATCGGACAAGTGTTTTACCTTCGTCCTTACGTTAATGAC TTCAGCACGGAATGGGTTGTTTCTTCTGTTACGTTGTTAACGCTTGGTTCTGTTCTCACTACATATATTGGAGAGAGAAT CTCTGATCTGAAACTTGGAAATGGCACTTCCCTATTGATTTTCACAAGTATCATCTCTTACTTGCCTGCATCATTCGGTA GAACAACAGCAGAGGCCTTACAGGAAGGAAACTATACTGGTCTTGGAACCATCGTTGTTTCATTTCTCCTCTTGGTACTC GGGATAGTATATGTTCAGGAAGCAGAGAGGAAAATTCCGCTCAATTATGCCTCGCGATACACCAGCAAAGCTGGTGGGCT TCAGAAATCTGCTTATCTTCCATTCAAGGTCAATAGTGCGGGAGTAATGCCTATCATATTCTCCACATCATCTCTTGCTC TTCCTGCTACTTTAGCACGATTTACCGGCATATCTGCTTTGAAGAATGTTGCTTTTGCTTTAACCCCTGGAGGTTCTTTC TATCTTCCAACCAATATACTTCTCATAGCTTTCTTCAACTACTACTACACTTTCTTGCAACTTGACCCGGACGACGTGAG TGAACAATTAAAACGCCAAGGTGCATCAATCCCTCTAGTCCGACCTGGTAAAAGCACTGCTCTCTTCATCAAAACCGTCC TTGGCCGGATATCGGTTCTTGGTTCTGCATTTCTGGCTGTTCTAGCTGCTGGTCCTGCTGTAGTCGAACAGATCACTCAC TTGACAGCATTCAGAGGATTTGCAGGCACTTCTGTTCTGATCCTTGTCGGGTGTGCGACAGATACTGCAAGAAAGGTTCA AGCAGAGATAATCTCGCAAAAGTACAAGAACATTGAGTTTTACGAGCTTGACAAGTATGATCCATGAACTGGACCTTCTG TTTTACATATAATACCCATCAAACCAAGTTACTGTGTTCTCTTGTCATAGATTGTAACAAATGTTTTAAACTTTGATTAG TATATA ................................................................................ .....N.......................................i..........i....................... ................................................................................ ...........N.................................................................... ................................................................................ .....................N.......................................................... ...............N................................................................ ................................................................................ ................................................................................ ................................................................................ ................................N............................................... ................................................................................ .........N.................................................................N.... ...........N.................................................................... ....................i........................................................... ................................................................................ ..........N...................................N................................. ..............................................N................................. .............................................N.................................. ................................................................................ ................................................................................ ................................................................................ ................................................................................ ........................................................N......N................ ............................................................N................... ...... Pos Score Pred ------------------------ 86 0.202 - 126 0.685 Yes 137 0.578 Yes 252 0.242 - 422 0.330 - 496 0.249 - 833 0.229 - 970 0.087 - 1036 0.150 - 1052 0.469 - 1141 0.599 Yes 1291 0.184 - 1327 0.160 - 1407 0.498 - 1486 0.313 - 1897 0.216 - 1904 0.224 - 1981 0.052 -

Explain the output -- Processing locus 195: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATACCTTCTTCAATTGCTGTTTTGCCATTTGCATATTATTTAACTTTCTTTGTTGGATCAGTCTGATTCTTTCTTACC AATTATTGTCTTAATCCTTGATTAAGAAAAAACAAAAAAACAAAAGACATGGAGTTTCTTCTCTTTCTTGGATGTATAAC AGTGTTTCTCTATGCAGGTAAGGGATTGTTGTTGCCAATGGGGTGGCTAACTAAAATCCTTAAAGGTTCTAGCCATAAGT TTTCTGATGGACAATGTAATGGAAGATATAGAGAAGATAGAAACTTGGAAGGTCCTCGTTATTCTGCGGAAGGATCTGAT TTTGACAAAGAGGAGATTGAATGTGCTATTGCACTTTCTCTTTCTGAACAAGAACATGTAATTCCACAAGATGACAAAGG AAAGAAAATAATCGAGTACAAATCTGAAACCGAAGAAGATGACGATGACGATGAGGATGAGGATGAAGAATACATGAGAG CTCAGCTAGAAGCAGCAGAGGAAGAGGAAAGACGGGTAGCTCAAGCTCAAATCGAGGAAGAAGAGAAAAGGAGAGCTGAA GCTCAACTAGAAGAAACAGAGAAACTGCTTGCAAAAGCTCGATTAGAAGAGGAAGAAATGAGGCGTTCTAAAGCCCAACT GGAGGAAGATGAGCTACTCGCCAAGGCCCTTCAAGAAAGTATGAATGTGGGATCTCCTCCTCGATATGATCCAGGAAATA TACTTCAACCATATCCCTTCCTCATTCCTTCTAGTCACAGGATATGTGTCGGTTGCCAAGCTGAGATAGGACATGGAAGG TTTCTGAGTGCATGGGTGGCGTTTGGCATCCTGAATGTTTCTGTTGCAATGCATTTCTCAATGTCAGGAAATCGGCCTTA TCACAAATTATGTTACAAGGAGCAGCATCATCCTAAATGTGATGTTTGCCATAACTTTATTCCGACAAATCCAGCTGGTC TTATTGAGTATAGGGCACATCCCTTTTGGATGCAAAAGTATTGTCCTTCACATGAGCGTGATGGAACTCCTCGTTGTTGC AGTTGTGAGCGAATGGAGCCAAAAGATACAAAATATCTCATTCTTGATGATGGTCGAAAACTGTGTCTTGAATGTCTCGA CTCAGCCATTATGGACACTCATGAATGCCAACCATTGTACCTTGAGATACGTGAATTTTACGAAGGCTTACATATGAAAG TGGAACAGTCAAATTCCTATGCTTTTGGTGGAGAGATCAGCTCTAAACGAGCTATGGAAGGAGAGAAACACGGACATCAT CATTTACCTGAAACTAGAGGACTTTGTTTGTCTGAAGAACAAACTGTCACCACGGTGTTAAGGAGACCAAGAATTGGGGC AGGCTACAAGTTAATAGACATGATCACAGAGCCTTGCAGGCTGATACGTCGTTGTGAAGTCACTGCGATTCTCATTTTAT ATGGACTTCCTCGTTTGTTAACCGGGTCAATCCTTGCTCATGAGATGATGCATGCATGGCTTCGACTAAATGGGTATCCA AATCTTAGACCAGAAGTGGAAGAAGGAATATGTCAAGTTTTAGCACACATGTGGTTGGAATCTGAGACTTATGCTGGCTC TACATTAGTAGACATCGCATCTTCTTCATCATCTGCTGTGGTGTCAGCATCGTCCAAGAAAGGTGAGAGGTCTGATTTTG AGAAGAAACTTGGTGAGTTTTTCAAGCATCAAATCGAGTCGGATTCTTCATCGGCATATGGGGATGGGTTCAGGCAAGGT AACCAAGCAGTTCTTAAGCATGGTCTGAGGCGAACCCTCGACCATATTCGCTTGACCGGTACATTTCCTAAATGGATTTA AACTATAAAAAGAGAGAAAACAAGCTTTGTTATAGTTTTTTAGAGTATAGACTTGAGAAGAAAAGAAAAAGAAAAATGTA TAGGTTACAACAATACACAC ................................................................................ ................................................i......................N........ ...........i.........................N.......................................... ......N......N....N............................................................. ....................N..................................N..............i......... ......................................i.....N.....N.....N.....N..........i...... ................................................................................ .........................................................i...................... ........N...............................i...N....................N.............. ...........................................N............................N....... ...........N......................N.............N...........N................... .........N..........................N....N...................................... .............................N.....................N........N................... ............i.................................N..N.....................N........ ..........N.........N...N................................................i...... ..................N..................................i.......................... ................................................................................ ...................N............................................................ N......................................N....N..N...N...i.............N.......... .............................N..................i.....................N......... ................................................................................ .........................................................N.....N................ ...................N...................................................N........ ...........................................................................N.... .................... Pos Score Pred ------------------------ 129 0.715 Yes 152 0.320 - 172 0.550 Yes 198 0.438 - 247 0.245 - 254 0.249 - 259 0.451 - 341 0.156 - 376 0.284 - 391 0.583 Yes 439 0.609 Yes 445 0.422 - 451 0.276 - 457 0.258 - 463 0.231 - 474 0.765 Yes 618 0.651 Yes 649 0.239 - 681 0.556 Yes 685 0.467 - 706 0.186 - 764 0.484 - 793 0.255 - 812 0.407 - 835 0.329 - 849 0.340 - 861 0.467 - 890 0.468 - 917 0.469 - 922 0.201 - 990 0.379 - 1012 0.319 - 1021 0.332 - 1053 0.729 Yes 1087 0.349 - 1090 0.486 - 1112 0.385 - 1131 0.342 - 1141 0.300 - 1145 0.262 - 1194 0.745 Yes 1219 0.396 - 1254 0.518 Yes 1380 0.398 - 1441 0.332 - 1480 0.493 - 1485 0.298 - 1488 0.419 - 1492 0.241 - 1496 0.588 Yes 1510 0.179 - 1550 0.400 - 1569 0.624 Yes 1591 0.146 - 1738 0.223 - 1744 0.326 - 1780 0.219 - 1832 0.345 - 1916 0.056 -

Explain the output -- Processing locus 196: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCCTGATTCCAGTAAAATGATACTTCTCTTTTTCTTCTACTATTTTTTGGGCACATTCTAGTAGTTTGACATTTTAATAA AATTTGGTTTGGATTTATTAGATGTTGCTCTCAGATATGGCTTATGAAGATGACGATGAGACACTTTTGAATGAAGAGGA GTGGAATTTAAGAGCATGCAGTGCAAAATTTATTGGTATTCTCGCTAATGTATTTGGAGATGAAATTCTTCTAACACTCA TGCCTCTTATTGAGGCAAAACTATCAAAATTCGATGATGAGACTTGGAAAGAGAGGGAAGCTGCAGTGTTTGCTTTTGGA GCCATTGCTGAAGGATGCAATAGTTTTTTTTACCCTCATTTGATTGTAGCAATCCTTCGTCGTCTTTTAGATGATCAATC TCCTCTCGTACGAAGGATAACGTGTTGGACACTTTATCAATTTGGCACATATGTTTTTGAGGAAAGTAATTTGGAGAATA GTAAACTCTTTACAAAAGTTCTTCATGGTTTTCGTTTCAAACTTTTGGATTCGAACATCTGGGTCCAGGAAGCTGCATGT TTGGCTTTGACAACTTTTGAAGAGGATGCTGGTGACAAATTAGTACCACACCTGGAAAAAATATTACAACAGCTAATGCG CGCTTTTGGAAAGTATCAGAAGAGGAACTTAAAAGTTCTTCTTGATGCTATTAGAGCACTAGCAGATTCAGTTGGAATCA ATCTGAACAAGCGTGCTTATATTAAGATTTTGATTCCCCCACTGGTTTCAACGTTGGAACAAATTTCCAATTCAGATAAA GATGTAATTCCATTGCTGAAGTGCTTCACATCCATTTCTAAGGCTTTAGAAGTAGGATTCGCTCCATTTTCTTGGTATGT TTTCGAGAGGTGCATGGATATCCTTCAACTACAACAGCTAGCTAAGGTTGATCATGATTTTGCCGGGGTTCAATACGACC AAAATATCGTCGTTTGTTCTCTTGAATTTTTTTCTGGACTTTCTAAAGGGCTTGTCAGTGGGATAGAGTCTCTGGTTTCA CAAAGTAATCTAAGGGATATGCTTCTTAAGTGTTTCATGGATGAAACTCCAGATGTTAGAGAAAGTGCTTTTGCCCTTAT TTGTCATCTTACAAAAAGTGCAAATTTTAGCGGAGAAAACCTTTCTGCTGCGAATAATGCTTGTAAAGCAATAGGAGAAT TAGCGGTCAAGTTTCTGGTAAAGAACAAACTCGTGAATGATATGAACTCAATGTTTCAGTATCCTCAAGAAGTCTCACCA ATTGTGACCAATGTTGTCTATTCATTAGGCATGATTATTCAACTCGGAGAGACACTAGAGTTGAAGAGTTTAACTACACT AGTTGAATACAATGCAATTGAACTAGCTATGAACAGTGCAATTACCGTTGGGATACTAGCACGGATTCGTCCAGACCTCA GTGCACGATCCATTGAGAACTTCATGAAGCCTTGGTGCATGAGATTGGCAACGTTAGATGATGACAGTACAAAAGAAAAT GCATTTCAAGGGTTATGTGAAATGGTTAAGGTGAATCCATCAAGATATGTCAGCTCCGTTGCTTTCATATGCTTAGCGAT CGCAAGCTGGAAAGATATGGAAAACAAGGTCATCCAAAGCGAATTCTCCAAAGTGCTGATAGGCTATAAAAACATGCTTG GAAAAAACTCATGGGAAGAATGTCTCTCTGTTTTGGATCCTCTTGCTAAGGAAAGGCTTGCTGCGAGGTATCAAGTGTGA CATTGATAGTAAATCGATTAGTCTCAAATGAGAGAGATCTCATTGCAAACAATATTCAGTGTTTTTGCAAAAGACGTTTC AGTTGCATTATTTTTATAA ................N............................................................... .....................N..............i......N.....N.....N..............N......... ...............N...............................N...........N...................N .................................N..N........................................... ..............N.......................................................N......... ..................................................N............................. ........................N...................................................N... .........................N.................................................i.... ............................................N................................... ................................................................................ .............N.......................................N.......................... ................................................................................ ..................i.................i...N...........N........................... ........................................................N....................... ....................................N....N........N............................. ..........N...................i................................................. ...........N................N................................................... .......................N..............N..................N..N.................N. ..............N......i........................N.....................N........... ................i........................................................N...... ..........N........i............................................................ ...........................N.................................................... ................... Pos Score Pred ------------------------ 17 0.332 - 102 0.485 - 117 0.526 Yes 124 0.398 - 130 0.232 - 136 0.145 - 151 0.174 - 176 0.384 - 208 0.283 - 220 0.134 - 240 0.499 - 274 0.242 - 277 0.183 - 335 0.181 - 391 0.181 - 451 0.490 - 505 0.128 - 557 0.409 - 586 0.366 - 636 0.623 Yes 685 0.076 - 802 0.385 - 877 0.203 - 894 0.263 - 934 0.241 - 1059 0.605 Yes 1077 0.540 Yes 1081 0.070 - 1093 0.244 - 1177 0.148 - 1237 0.233 - 1242 0.156 - 1251 0.205 - 1291 0.111 - 1311 0.534 Yes 1372 0.281 - 1389 0.405 - 1464 0.193 - 1479 0.239 - 1498 0.340 - 1501 0.451 - 1519 0.459 - 1535 0.397 - 1542 0.679 Yes 1567 0.305 - 1589 0.212 - 1617 0.502 Yes 1674 0.451 - 1691 0.361 - 1700 0.563 Yes 1788 0.097 -

Explain the output -- Processing locus 197: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGTGAGAACGAAACTGGATTCAGGGTAAAACCTTCATCAATCTTTAGAAGCTTTATCAATGGATTTGTGTTGAATTTGTA TTGAAAGTTTTGATCTTTTAGGTGTATAGATGGATGACACAACAGAATTGGTACCGGTTTGATTTCGGGTTAACGACGAA GCTAGCTGAATACTTGGGGAAAGAACGGAAATTTACGAAATGTCGAGAGGTATTTGATGATGTTTTGAATCAAGGACGTG TACCAAGTGAATCTACATTTCATATTCTTGTAGTTGCGTATCTAAGTAGCTTATCAGTAGAAGGTTGTCTCGAGGAAGCG TGTAGTGTTTACAATAGAATGATTCAACTTGGAGGTTACAAACCGCGTCTTAGTCTGCATAACTCTTTGTTTAGAGCTTT GGTTAGTAAACAAGGAGGGATTCTGAATGATCAGCTTAAGCAAGCAGAGTTTATCTTTCACAATGTTGTGACAACCGGGC TTGAGGTTCAGAAGGATATTTATAGTGGATTGATCTGGCTGCATAGTTGTCAAGATGAAGTTGATATAGGTAGGATAAAC TCTCTAAGAGAAGAGATGAAGAAGGCCGGTTTTCAGGAGAGTAAAGAAGTTGTGGTATCGTTACTTAGAGCTTATGCAAA GGAGGGAGGTGTGGAAGAAGTTGAGAGGACGTGGCTTGAATTGCTTGATTTAGATTGTGGTATACCTTCTCAGGCGTTTG TGTACAAAATAGAAGCTTATTCGAAAGTCGGCGATTTTGCGAAAGCTATGGAGATATTTAGGGAGATGGAGAAGCATATA GGTGGTGCAACTATGTCTGGATACCATAAAATCATTGAGGTTCTATGTAAAGTCCAACAAGTGGAACTTGTGGAAACTCT CATGAAGGAGTTTGAAGAAAGCGGGAAGAAGCCGCTTCTACCATCATTCATCGAAATAGCCAAAATGTACTTCGATTTGG GTTTACATGAGAAATTGGAGATGGCTTTTGTTCAGTGCTTGGAGAAATGCCAACCTAGCCAGCCTATATATAACATATAT TTGGATTCATTGACTAAAATAGGCAACCTTGAGAAAGCAGGGGATGTCTTCAATGAAATGAAAAACAACGGGACAATCAA TGTGAGTGCTAGATCCTGCAACAGCCTTTTAAAGGGATACCTAGATTGTGGAAAACAAGTGCAGGCAGAGAGAATATATG ATCTGATGAGAATGAAGAAATACGAAATCGAACCACCGCTTATGGAAAAGCTTGATTACATCCTGAGCTTGAAGAAAAAA GAGGTGAAGAAGAGACCGTTTAGCATGAAGCTAAGCAAAGACCAGCGTGAGGTATTGGTAGGTTTGTTGTTAGGTGGCTT GCAAATCGAATCAGACAAAGAGAAGAAGAGCCACATGATCAAATTTGAATTTAGAGAAAATTCTCAAGCTCATCTGGTTC TTAAACAAAACATACATGACCAGTTCCGTGAGTGGTTGCATCCTTTGAGCAATTTTCAGGAGGATATTATACCGTTCGAA TTCTACTCCGTTCCCCATTCATACTTCGGGTTTTACGCTGAACATTACTGGCCAAAGGGTCAGCCAGAGATTCCAAAACT GATTCATCGGTGGCTATCGCCACACTCACTCGCGTATTGGTACATGTACAGCGGCGTTAAAACATCATCAGGAGACATTA TCTTGAGATTGAAGGGAAGTCTCGAAGGTGTTGAGAAAGTAGTAAAGGCTCTTCAAGCCAAATCTATGGAATGTCGAGTT AAGAAGAAAGGAAAAGTCTTCTGGATTGGACTTCAGGGAACAAACTCAGCTTTGTTCTGGAAACTAATAGAGCCTCATGT GTTAGAGAACTTGAAAGAGCATTTGAAACCTGCTTCTGAATCACTGGACAATGTTAAGGAAGCAGAAGAACAAAGCATCA ACTTCAAATCAAACTCTGATCACAGTGACGATTGTGTCAATTCAGAAGCACATTTTTACTGAAAAGGCAACCAAGTTTAT TTTTTTTAACTTGTTGGGGTTTGATATATAATAAAACGTCGTTAAATTAGTAACTAAATTCAACCTGACCACTTGAAGAA ..........................................................i..................... .............................i...N.............................................. .......................................N................N..N.................... ................................................................................ ..................N............................................................. ..........................N...................................N................. ......................................................N......................... ...............i.........................................................N...... ................................................................................ ...............................................i.................i.............. ............i...............................i................................... ......N.............i.........................N................................. ...........................................N........N....N.....................i .............................................................................N.. .....N.....N.............................N...................................... ........................N....................................................... ..................................N............................................. ...............N................................................................ ................................................................................ ...........................................N.................................... .................................................................N....i......... ............................................................................N... ..................................................i............................. ................................................................................ ................................................................................ Pos Score Pred ------------------------ 59 0.763 Yes 110 0.602 Yes 114 0.185 - 200 0.286 - 217 0.252 - 220 0.341 - 339 0.349 - 427 0.078 - 463 0.234 - 535 0.163 - 576 0.799 Yes 634 0.151 - 768 0.603 Yes 786 0.573 Yes 813 0.734 Yes 845 0.571 Yes 882 0.463 - 945 0.247 - 967 0.218 - 981 0.771 Yes 1007 0.345 - 1084 0.361 - 1093 0.412 - 1098 0.401 - 1120 0.547 Yes 1198 0.333 - 1206 0.432 - 1212 0.332 - 1242 0.363 - 1305 0.283 - 1395 0.270 - 1456 0.490 - 1644 0.344 - 1746 0.173 - 1751 0.559 Yes 1837 0.267 - 1891 0.647 Yes

Explain the output -- Processing locus 198: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTCTCTCTCTCTCTCTCCCTTCTCTTATCATGGTCGACTGGTTCTCAAAAGTCTGATGATTGGTTGTGATTAGTTCCG ATTACTTATTAGTATAAGTAGATCGTTGCCTTATTGGATCATCGAAGCTCCTGTTTTGATTTCCCACTGAAAAATCGATA CTTGGGCATGCAAATACTTGTTTACTTGAAACAATGTTACTTGTGTTCTTTTAGACTGAAAGACGAGGACATGGAAACGA GAATAGAACTGAAGAAGAAAATTTCCGAGATTTTCAAAGACTTTATGACAGTAATCACGCAGCTTGAAGAACTAGGGAAT GCTGCAAACAACTTTCTTCTACGGTTTCAGCAAGGACTAAGTTTTCTTCAACGCCCTCCAATAGTTACTTCGTCCAAGTT GATTGAGAACATTATCAAGAAGAATGAAACAAGGCGGCTTAAATCATATATCGAGGCAGGATGTATTAATATCCATGATG CTGCACAAAGCACAAGAGCTTATGTGAATGTTGCAGCCAAAAACTTGTTGATTGAGCTGGAACGTCTCACTGATGAGGCC GCCCTTGCAATTGAGTCTGCCACACAGCTAGATAAAGACTCAAGTGATGGATTGCAACAAGTAACATGTGATGAGGAGAA CGAGACTGTACAATTACCTCAAGGACCTGAAGTTACAGAGTACGCTGCACTTATTGCAGTTATTTACAGTATGATGAAGC AGAACTATGTTATGCAGGATAAGATTGTGAGATCGCTTAGTTTGAAGTCATCATCTGGTGAGCTAGAGACTTACAGTCTG ATGTGGTCATTGCGCCCCGGCGTCTTCGAATTTTTCGTGGCTACGTTTGACGGTGCCACGAAGAAGAAGAAGACGAAGAT GGATTATCAGAACAAGCTTGTTCTTGCTCCCATGGTTCGTGTGGGAACGCTCTCTTTCAGGATGTTGGCGGCGGAATACG GCGCCGATATCACATACGGTGAAGAGATTATTGATCATAAGCTTGTTAAATGCGAACGCCGACTCAATGTTGCTTCTGGG ACATCTGAGTTTGTGGAGAAAGGAACAGATAATGTAGTCTTTAGCACATGTGATGAAGAAAAAAGTAGGGTTGTTTTTCA GATGGGAACTTCTGATGCTGTTAGGGCTCTAAAGGCTTCTGAGATTGTGTGTAATGACGTTGCAACTATTGATATTAATA TGGGTTGCCCCAAGGCATTCTCTATCCAAGGTGGAATGGGTGCTGCTTTGTTAAGTAAACCCGAGCTAATTCATGATATT TTGGCAACACTTAAGAGGAACTTAGACGTACCGGTTACTTGCAAGATTCGTCTATTAAAATCACCAGCAGATACTGTTGA ATTGGCTAGGAGAATCGAGAAACTTGGTGTACCAGCTCTTGCTGTACATGGGAGGAAAATTGCAGACAGGCCAAGAGATC CTGCTAAATGGGATGAGATTGCAGATGTGGTGGCAGCATTATCCATTCCCGTCATTGCAAATGGCGATGTTTTAGAATAT GATGATTTTTCTCGCATTAAAACCGCAACAGGCGCTGCATCAGTTATGGTTGCAAGAGGAGCTATGTGGAATGCTTCAAT TTTCTCTCCAAAAGGAAAATCACACTGGGAAGATGTGAAAAAGAAATACCTTAGAAAGAGCATCTTGTGGAATAACGATG TTAAGAGCACGAAATACACGATAAAGGAGATGATAGCGCATCATTCTTGTCTTGAACTACCGGAAGGGAAGTCTATCAAC AAAGCAGACACTTTAGAAGATTTAGCGCGGCTTTACGATTTAGAAGATTACTTTTGGACAGTGAAGAACATTGGTCCACT GACACATAACTTAAACCATGTTTTATGATACTGAAAGAAAATGCCAACACTTTGCATTTTCATTTATATCATTTTGTAAT CTCTATTGATTTTTATATATAGAGGCATCCCATTGTTTTTACTCAAA ...............................N.........................N...................... ................................................................................ .......N.........................N....................................i......... ............................................N.................................N. ................................................................................ .......................N....................................N.............N..N.. .....................N.....N............................................N....... ..............................................N..................i....N......... ......................................................................N..N...... ......i....N.................................................................... N.............................................................................N. ...............................i.............................i.................. .................................................N................N............. ...............................i...............N....N........................... ...................................N....................................N....... ................................................................................ ...............................................N................................ .......N....N...........N...................................N.....N...........N. ................................N............................................N.. .............................N.................................................. ................................................................................ .................N......N...............N....................................... ............................................... Pos Score Pred ------------------------ 32 0.378 - 58 0.267 - 168 0.405 - 194 0.268 - 231 0.659 Yes 285 0.266 - 319 0.280 - 424 0.468 - 461 0.495 - 475 0.097 - 478 0.151 - 502 0.188 - 508 0.167 - 553 0.221 - 607 0.454 - 626 0.525 Yes 631 0.149 - 711 0.189 - 714 0.295 - 727 0.528 Yes 732 0.322 - 801 0.254 - 879 0.422 - 912 0.500 Yes 942 0.804 Yes 1010 0.289 - 1027 0.167 - 1072 0.510 Yes 1088 0.166 - 1093 0.105 - 1122 0.767 Yes 1135 0.125 - 1174 0.151 - 1200 0.684 Yes 1236 0.445 - 1273 0.157 - 1408 0.173 - 1448 0.494 - 1453 0.186 - 1465 0.205 - 1501 0.237 - 1507 0.101 - 1519 0.161 - 1522 0.115 - 1566 0.431 - 1584 0.544 Yes 1591 0.255 - 1633 0.185 - 1678 0.297 - 1710 0.361 - 1858 0.152 - 1865 0.441 - 1881 0.083 -

Explain the output -- Processing locus 199: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCCTTTAGACGGAAACAAAAAAGAAACCCCACATGAAAAGTAGCCAACGTTCAAATTACAGGTGAAACAAATTCACCA GAAAATCTGAGAAATTGTTAAGATTTCTGTATTGAATTAAAGAGAAAAAAGGTGGAAACTTTATTGCTTTTTGGCCATAA AAAGATGGAAAGTAAAGTAAGAGGAGAAGAAGAGAGACAAAAAGTGGAAAGATTGGTGGAAAAGTTAAAGGTTCATAATA ATAATAATAATCGATCTTCAACTCCGGCGCACATTAGCTTCGTTCCTAACTCAATCGTTGTGTCCTCTAGAAAGCTCGCT GCTGCTTTCTGGGAGTTTCATCAGTACCACTACAAAGACGAAGAAGATTGCTCTTACTCTTACCTCTCCTCTGCTTCTGC TAAAATGCACCGCGGCCCAAATGGTTTCGCCGGAGCTAGTAGCCGCCGTCAACGTCACGGAAAGGCGGTTGCGGTTAAGG AGAATGGTCTTGACCTTTCTCAATTCCTTCGAGATCCTTCTCCTGATCATCAGCCAGATAGTGCGGGAAGTCTAAGGAGA CAGATTGGTCAAATGTTGATAAAGCATCATCAATCAATCGATAGAAACAATCACGCTCTTCAGCCTGTATCTCCTGCTAG TTACGGAAGCTCTCTCGAGGTTACGACATACAACAAAGCAGTAACTCCAAGTAGTTCATTGGAGTTCCGAGGAAGACCGT CTAGAGAACCTCACTACAATCTCAAGACATCAACCGAACTTCTTAAAGTACTGAACCGTATTTGGAGCCTTGAGGAGCAG CATGTCTCCAATATCTCCTTGATAAAAGCTTTGAAAACCGAGGTGGCTCATTCACGCGTTCGAATCAAGGAGCTTCTTAG GTACCAGCAAGCCGATAGACACGAGCTAGACAGTGTGGTGAAGCAACTCGCTGAAGAAAAGTTGTTGAGCAAGAATAAGG AAGTTGAGCGGATGAGCTCTGCTGTTCAGTCCGTGAGGAAAGCGTTAGAAGATGAGCGGAAACTTAGAAAACGTTCTGAG AGTTTGCATAGGAAAATGGCAAGAGAGCTCTCGGAAGTGAAGTCTTCGTTGTCTAACTGTGTCAAGGAGCTTGAGAGAGG GTCTAAATCAAATAAGATGATGGAGCTACTCTGCGATGAGTTTGCCAAAGGGATCAAGAGTTACGAAGAGGAGATTCACG GTTTAAAGAAGAAGAATCTTGATAAGGATTGGGCGGGTCGAGGTGGAGGAGATCAGTTGGTTCTTCATATTGCGGAATCT TGGCTTGACGAAAGGATGCAGATGAGATTAGAAGGTGGAGATACATTGAATGGTAAGAACAGATCAGTGCTGGATAAGCT GGAGGTTGAGATCGAGACATTTCTTCAGGAGAAGCGGAATGAAATACCGAGAAACCGCCGGAATTCACTTGAATCTGTTC CGTTTAACACCTTGAGTGCACCACCGCGGGATGTAGACTGTGAAGAAGACTCTGGAGGAAGCGATTCTAATTGCTTTGAG CTCAAGAAACCTGCAGAGTCTTATGGAGACGAAACTAAGAAACCAAACCAACATAACAAAGACGGTTCGATTGATGAAAA GCCGAAAAGCCCGTCGAGTTTCCAAGTGAACTTTGAGGATCAAATGGCGTGGGCGTTATCGAGTAACGGAAAGAAGAAAA CCACCCGAGCTATTGAAGACGAAGAAGAAGAAGAAGATGTAAAGCCTGAGAACAGCAACAACAATAAGAAACCAGAAAAT GAGTGTGCTACTACGAACAAGAACGATGTAATGGGGGAAATGATACGAACACACAGAAGGTTATTGTCCGAAACTCGTGA AATCGATGAAGCGTCTTGCAATTTCCCATCTTCTCGAAGACAAGCAAGTCCAGTTCGACAATGGATTTCAAGAACCGTCG CACCTGATCTTCTAGGCCCGTCGGAGATTGCGATAGCTCATGGAGTAAAGGATAATACTTTGAAAACTAAGCTAGCAAAC AGTTCAAAGTCGCGTCTTCGGTTATTCAAAGGCTAATCTATCCAGAGTGGTTCGGTCGAAAACGGACAAAAACGAGAGTT CTTGTTGGATACACACAGATCCCTGAGTTTCATTCATATTCACGTTCTTTGTAAA ..................................N............................................. ................................................................................ ....i........................................................................... ................................................................................ ................................................................................ ....i...............N........................................................... ...N............................................................................ ............i................................................................... ................................................................................ ................................................................................ ................................................................................ ...........N.......................................N............................ ...............i................................................................ ................i..i...............N............................................ ................................................................................ ...............N.....N...........................N.............................. ......................................i......................................... ..............................N................................................. ......................N..................................................N...... ...........................................i.................................... ....................................N.........................................N. .........................i....i........N........................................ .....N......................................................i................... .......................................N........................................ ................................................................................ ....................................................... Pos Score Pred ------------------------ 35 0.343 - 165 0.638 Yes 405 0.870 Yes 421 0.356 - 484 0.383 - 573 0.715 Yes 802 0.517 Yes 972 0.343 - 1012 0.339 - 1056 0.512 Yes 1137 0.608 Yes 1140 0.602 Yes 1156 0.348 - 1296 0.318 - 1302 0.459 - 1330 0.266 - 1399 0.502 Yes 1471 0.400 - 1543 0.265 - 1594 0.121 - 1644 0.748 Yes 1717 0.348 - 1759 0.475 - 1786 0.500 Yes 1791 0.631 Yes 1800 0.464 - 1846 0.204 - 1901 0.679 Yes 1960 0.303 -

Explain the output -- Processing locus 200: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGACCAAGTTGGTCCAACTCTAGCTATACCTTCCACACTGCTTCCTCCCCCACCACAAAAGGGGTACTATCGTCATTTCA CACGTACCTACCTGTAATTAATGTGACATTATAAATAACAACGTGAAAGAAGGATCCATGCAGAAACAATTAGCATATAT TACAAAACACATACTAATTGTTTCTCCTTCTCCTTCTTCCTTGCAAATGGATACTTTAGCTTCAAACTCTTCGGATCTCA CGACCAAGTCAAGTCTCGGAATGTCGTCGTTTACCAACATGTATCTCCTCACAACGCTTCAAGCCCTTGCGGCCCTTTGC TTCTTGATGATTCTCAACAAAATAAAGTCATCTTCACGAAACAAGAAGTTGCATCCTCTCCCGCCAGGACCCACCGGTTT TCCAATTGTTGGAATGATTCCGGCGATGCTTAAAAACCGTCCAGTTTTCCGGTGGCTCCACAGCCTCATGAAGGAGCTTA ACACGGAGATAGCTTGTGTGCGTCTAGGAAACACTCACGTGATCCCTGTGACATGTCCTAAGATTGCACGTGAGATCTTC AAGCAACAAGACGCACTCTTTGCGTCAAGACCACTCACTTATGCTCAAAAGATTCTCTCTAACGGCTACAAAACCTGCGT GATCACACCGTTTGGCGAACAATTCAAGAAGATGAGGAAAGTGATCATGACAGAGATTGTTTGTCCAGCGAGACACCGTT GGCTACACGACAATAGAGCTGAGGAAACCGATCACTTGACCGCTTGGCTCTACAACATGGTTAAGAACTCTGAACCAGTC GATCTCCGTTTTGTTACGAGGCATTATTGTGGAAATGCAATCAAGAGGCTTATGTTCGGGACGAGGACGTTCTCGGAGAA AACCGAAGCCGATGGTGGACCGACTTTGGAAGATATCGAGCATATGGATGCTATGTTCGAGGGGTTAGGGTTTACGTTTG CGTTTTGTATATCGGATTATCTACCGATGCTTACGGGATTGGATCTAAACGGACATGAAAAGATTATGAGAGAATCCAGT GCAATTATGGATAAATATCACGATCCTATAATTGATGAGAGGATTAAGATGTGGAGAGAAGGTAAGAGAACTCAGATCGA AGATTTTCTAGACATTTTCATCTCTATCAAGGACGAAGCTGGCCAGCCTTTGCTTACCGCTGATGAAATCAAACCAACCA TTAAGGAACTTGTAATGGCGGCGCCAGACAACCCATCAAACGCCGTGGAATGGGCCATTGCGGAGATGATAAACAAACCG GAGATCCTCCACAAAGCTATGGAAGAGATCGATAGAGTCGTCGGAAAAGAAAGATTCGTCCAAGAATCCGATATACCAAA ACTTAACTATGTTAAAGCTATTATCCGAGAAGCTTTCCGTCTTCATCCCGTCGCCGCCTTCAACCTCCCACACGTGGCAC TCTCTGATACGACCGTCGCAGGTTACCATATTCCTAAAGGAAGTCAAGTTTTACTTAGCCGTTACGGTCTTGGTCGTAAC CCCAAGGTTTGGTCTGATCCACTTAGCTTCAAACCAGAAAGGCATCTCAATGAGTGTTCGGAAGTGACTTTGACGGAGAA TGATCTCCGTTTTATCTCGTTTAGTACCGGAAAGAGAGGATGTGCTGCTCCGGCGTTAGGTACGGCGATAACGACCATGA TGCTAGCGAGGCTTTTGCAAGGGTTTAAGTGGAAACTAGCAGGAAGTGAGACACGTGTCGAGCTTATGGAATCGAGTCAT GATATGTTTCTTTCGAAGCCTTTGGTGTTGGTCGGAGAATTGAGATTGTCGGAGGACCTTTACCCAATGGTGAAGTGAGG CGACGGTGACGTACGGCGAGGATAATTCCGTATATACTATGGATAACCCAAAGTTTAGTGAATCACAAACCAAACTTTGT TCGGTTTTGGTCCTTTT ................................................................................ ....................N....................................i...................... ..............................................i................................. ....................i.................N......................................... ......N......................................................................... .............N...........N.........................................i............ ....................................................N........................... ........................................N....................................... ...............................i..............i................................. ........................................................N....................... ..................................N................N............................ ...........N...............................i...N....N........................... ..........................N...........................N..........i.............. ......i...........................N.............i............................... ..............................................................N................. ..............i..................................i...............i.............. ..................i............................................................. ........N....................................................................... ................................................................................ .................................................N.............................N .......................................i....................................N..N .................................................................N............N. ...N..............................................................N............. ......................................N......................................... ................. Pos Score Pred ------------------------ 101 0.217 - 138 0.600 Yes 207 0.574 Yes 261 0.585 Yes 279 0.348 - 327 0.302 - 414 0.384 - 426 0.464 - 468 0.528 Yes 533 0.369 - 601 0.164 - 672 0.764 Yes 687 0.812 Yes 777 0.479 - 835 0.106 - 852 0.481 - 892 0.221 - 924 0.557 Yes 928 0.061 - 933 0.302 - 987 0.490 - 1015 0.114 - 1026 0.514 Yes 1047 0.505 Yes 1075 0.100 - 1089 0.810 Yes 1183 0.089 - 1215 0.874 Yes 1250 0.733 Yes 1266 0.508 Yes 1299 0.558 Yes 1369 0.476 - 1570 0.401 - 1600 0.173 - 1640 0.748 Yes 1677 0.417 - 1680 0.381 - 1746 0.329 - 1759 0.481 - 1764 0.345 - 1827 0.348 - 1879 0.072 -

Explain the output -- Processing locus 201: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTCTGTTAAACAAACGAGGTTTTATTTTTTAAACAACATATCACAAATTCACAATAGTTGTTTAACTTTTTTGTTTTT TGTTTTTTTCTATGACTATAACACATGAAAAGGTCAGATATGTATCATCATCTTCAAACGAGGATTTTTCTCCACCAACA AAATGATTTGCTGAGAGCAGAAAACAGAGCTAGGATTCATGCTATGACCTCTCCATCAATCTGCAGATCTTGTGAGGAGC CCATCATTTCAACCGAAGAGAGGGAGCTATGGTTGGAGAATGCTCGTCTTCGGTCCGAGATTGATACATTGACTTGTTTC ATATGGAGATTAAATTCGTTCAGGAACCTATATCCCGCATTTGCTACGTCTTTGACTGAAGTGGGTTACGGAGTGGCGGT TATGACGTCACTTTCGCTGAAAGAAGTTGTTTTTCTTGCGAGGCAAAGAACTCCGATGTGGACAAGTAACGGGAGATTGA ATCTCGACGAATACTACTCTAAATTATTTCCTTGGTACGCAAGAAACGCACCCGGGTTTGTACATGAGGTATCAAGAGCT TCTGCTTTTGTCCCATGTGATGCTTCATCGCTCGTGGCAAATCTTATGAATCATGTAAGTTGGCAAAAAATCTTTCCGTC AATCATCGCGGATGTGTCTGTGGAATCACAACAACGGGGATTGCAAAAGATTAATGTGAATTTTATGCCACAAATCTCTC CTCTCATTCAAACTCGGAATGTGAAACTTCTTCGACGCTCGAGGCATATAGAGGATGATACATGGGCTATTGCAGAAATT TCTATGTATTTCAGCTCATATGCGCAACATTTACGTCCTGAATATATGAGATTTCCTTCTGGATATCTTATCCAACATAT AGCCAATGGTATCTCTAAGGTAACCATTCTCGACCATTGGGTTTACAAGGAAGAAGAAGGCATGAACACTTTCAATTCAA ACTCTGAATTTGGTGCACAGAGATGGCTCACTGCTCTCCAAAAGCATTACTACAACACCTGCCCCGTTTCTATTCCTTCA ATTGGTCACAATATTCAAATATTCGACCAGATCTGTCGCAAGAACTTGCTTAACCTATCTTCATTTATGGTTAACGTATT TTGTTCGGGAGTTTGCGGGATAACTGGACAAAGGTGGAATCGGTTGAATACAGTTGGAGTTTCGGCTAATAACATTAGAA TGTTCACGCAAGAAAGTCGAGGCATGAGTGGGATCCCTTGCGTTTTGGTTAGCGCTACCGGTTTAGCTAGAATGCACACT AAACCAGAAGTAATGTTTGGATTAATCAACGGTGCAGAAAAACAAGAAATATGGAGTTATTTAGAGTCTGCTAAAGATAT GAAAGAACTAATTCGTATCGGAAGACATCCTAATTCATGGAATGAAGTCTCTGTTTTCAGCATTGAGTGGAAGGGTTCGA AGGAGTGGTATCTGATTCAAGAAACATACTACGATGAATCAGGAGCAATGATAATCCACACTTGCGTAGAAGCGCCATAT TTTGCAGCTGCAATAAACGGTGGGGATTTGTCTGGCGTCGAACTGTTACCCAGTGGATTCACCATAATACCATGCGAATC ACAAGAATGTTTCGTGACAGCTAGTTGTTATGTTAAGGCTGACCAAACTATGGTCACGAGTCCAAATGAGCTGGGGAGTT ATATGGAGAACATGGTCACTAACATTCTAGGAAATGTCCAAAATGCTCTTCCAGTCCACCGTTGAAGTTTTATGGCTTAT GAAGTTTTTACCGTTCTTGTTGTCGTCGTCGTCGTTATTGCTATTGCTGCATGTTGTTGTTGTTGTTGTTGTTGTCGTTG TCGT ................................................................................ ...........i............i..............i........................................ ..i...................................N....i.................................... ............................i..........N........................................ ..N............................................................................. ...............................................................N................ ..............N....N.........................N......N........................... ...........N.........................................N..........N............... ..................N...................................N......N.................. ...N...............N.........................i.................................. .....i.......................................................i.................. ......................i......................................................... ..................................................................i............. ...............................................................................i .......................i...............................................N........ ............N.....................................N...........................N. ....................................N....N...................................... .................................N.............i................................ .......................................................................N........ ......N......................N...................N...............N.............. ..N........i.....................N........N............................N......N. ..................................................N............................. .... Pos Score Pred ------------------------ 92 0.590 Yes 105 0.748 Yes 120 0.540 Yes 163 0.634 Yes 199 0.239 - 204 0.521 Yes 269 0.504 Yes 280 0.355 - 323 0.490 - 402 0.156 - 456 0.647 Yes 544 0.311 - 575 0.209 - 580 0.343 - 606 0.398 - 613 0.249 - 652 0.150 - 694 0.351 - 705 0.174 - 739 0.198 - 775 0.191 - 782 0.206 - 804 0.349 - 820 0.241 - 846 0.667 Yes 886 0.541 Yes 942 0.824 Yes 983 0.560 Yes 1107 0.722 Yes 1200 0.515 Yes 1224 0.615 Yes 1272 0.312 - 1293 0.394 - 1331 0.378 - 1359 0.393 - 1397 0.465 - 1402 0.341 - 1474 0.209 - 1488 0.644 Yes 1592 0.361 - 1607 0.486 - 1630 0.100 - 1650 0.455 - 1666 0.152 - 1683 0.366 - 1692 0.637 Yes 1714 0.085 - 1723 0.074 - 1752 0.419 - 1759 0.137 - 1811 0.046 -

Explain the output -- Processing locus 202: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACCAAAACAACTATTCAATTCCATCATGTGAAAAAATAATTGTAATTAGTTGTAAACTCAACCACCTTATCTTATCTTAT AAAATTCATACTAGTGTCTAAGCACGTTACTTCCATATTATATTGTCATCGACTAATCAAATGAATCTTGTTTTGACAAT CCTTTATAAGTATATAAACACATTTTCTTCATTGACGCTAAAAAGAAAAAAAACACAAAACTCATCCATTTGGCTTCTCT CTTGCATATTCGCTGCAAGGATGGGAAATTCAAAACCACTCCCTACAATTTCTTGTATTATAGTTTCAGTCTTGTATTTT TCATTCTATTGCATAACACCAACTTCTTCATCAGCCTCCATCCAAGATCAATTCATAAACTGTGTCAAAAGAAACACACA TGTTTCTTTTCCACTCGAGAAAACGTTATTCACCCCTGCGAAAAACGTCTCTTTGTTCAACCAAGTCCTTGAATCGACGG CTCAAAATCTCCAGTTCTTGGCAAAATCCATGCCTAAACCGGGATTCATATTCAGACCGATTCACCAGTCTCAAGTCCAA GCTTCCATCATTTGTTCAAAGAAACTCGGAATTCATTTTCGTGTTAGAAGTGGCGGTCACGATTTCGAGGCCTTGTCTTA TGTTTCACGGATTGAAAAACCGTTTATATTACTCGACCTGTCAAAATTGAAACAAATCAATGTTGATATTGAATCCAATA GTGCTTGGGTTCAACCTGGTGCTACGCTTGGTGAGCTTTACTACAGAATTGCAGAGAAGAGCAAGATCCATGGATTTCCC GCGGGTTTGTGCACAAGTGTAGGCATAGGTGGGTATATGACAGGCGGTGGATACGGTACCTTGATGAGGAAGTATGGTCT TGCGGGAGATAATGTTCTAGACGTAAAGATGGTTGATGCAAATGGTAAATTACTCGACAGAGCCGCGATGGGTGAGGACC TATTTTGGGCGATTAGAGGAGGCGGTGGAGCGAGTTTCGGGATAGTTCTAGCATGGAAGATCAAGCTTGTTCCTGTTCCT AAGACTGTTACCGTCTTCACTGTCACCAAAACGTTAGAACAAGACGCAAGATTGAAGACTATTTCTAAGTGGCAACAAAT TTCATCCAAGATTATTGAAGAGATACACATCCGAGTGGTACTCAGAGCAGCTGGAAATGATGGAAACAAGACTGTGACAA TGACCTACCTAGGTCAGTTTCTTGGCGAGAAAGGCACCTTGCTGAAGGTTATGGAGAAGGCTTTTCCAGAACTAGGGTTA ACTCAAAAGGATTGTACTGAAATGAGCTGGATTGAAGCCGCCCTTTTCCATGGTGGATTTCCAACAGGTTCTCCTATTGA AATTTTGCTTCAGCTCAAGTCGCCTCTAGGAAAAGATTACTTCAAAGCAACGTCGGATTTCGTTAAAGAACCTATTCCTG TGATAGGGCTCAAAGGAATATTCAAAAGATTGATTGAAGGAAACACAACATTTCTGAACTGGACTCCTTACGGTGGTATG ATGTCGAAAATCCCTGAATCTGCGATCCCATTTCCGCATAGAAACGGAACCCTCTTCAAGATTCTCTATTACGCGAACTG GCTAGAGAATGACAAGACATCGAGTAGAAAAATCAACTGGATCAAAGAGATATACAATTACATGGCGCCTTATGTCTCAA GCAATCCAAGACAAGCATATGTGAACTACAGAGATCTAGACTTCGGACAGAACAAGAACAACGCAAAGGTTAACTTCATT GAAGCTAAAATCTGGGGACCTAAGTACTTCAAAGGCAATTTTGACAGATTGGTGAAGATTAAAACCAAGGTTGATCCAGA GAACTTCTTCAGGCACGAGCAGAGTATCCCACCTATGCCCTACTAGAAGCTAGGTTCATGAAACCAATAACATTATCAAA AATAAGAATAAATGATAATTGTATACAACATGATTCGTCTTTCTTTATTTCAGACAATGTGGACA .........................N...................................................... ............................................................N................... ................................................................................ ....................N........................................................... ...............................................................................N ................................................................................ .............................N.................................................. ...............................................................................N ...........................................................i.................... .....................................................................i.......... ....................................i..........................i.........N...... ...........N................i......N.....N.........................i............ ....................................................N........................... ................................................................................ ........................................................N..N...................i ..................................................N............................. .....................N...........................N.............................. ................................................................................ .............................................................................i.. N............................................................................... ........N....................................................i.........N........ ..................N............................................................. ................................................................................ ..................................N......................N...................... ...........N.................N..........................N........ Pos Score Pred ------------------------ 26 0.149 - 141 0.344 - 261 0.279 - 400 0.285 - 510 0.319 - 640 0.091 - 700 0.519 Yes 790 0.605 Yes 837 0.641 Yes 864 0.594 Yes 874 0.294 - 892 0.412 - 909 0.736 Yes 916 0.230 - 922 0.345 - 948 0.661 Yes 1013 0.404 - 1177 0.182 - 1180 0.245 - 1200 0.736 Yes 1251 0.412 - 1302 0.282 - 1330 0.492 - 1518 0.616 Yes 1521 0.419 - 1609 0.351 - 1662 0.751 Yes 1672 0.157 - 1699 0.498 - 1875 0.262 - 1898 0.084 - 1932 0.106 - 1950 0.060 - 1977 0.052 -

Explain the output -- Processing locus 203: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGCAAATGCATGAAGTACGGCGCAAAAGAGCATTGGTGCCTTTCTGTACATATGCATGCATGGGGATTTACAATATGAAC CTACTTCCTCACTTCCCGTAATTACTTCTCTATTCCATACTCACTTCGTCACTTGTATTAAAGCTTAACCTTGGTGCAAT CTCAATTACACTTTTAAACTCTATGTTGATCTCAACAATGAAGCAGAAACATCTACTGCTTCTAGGATTCTTATTAGCTT ATTGCTTCAGCTCTGTTTTTGTGATAAACGCAGAGGACCCCTACCTTTTCTTCACTTGGACTGTTACTTATGGAACCAGA TCGCCTTTAGGTGTTCCTCAACAGGTTATTCTTATTAATGGCCAGTTCCCTGGTCCTCCAATTGAAGGTGTAACAAACAA TAATATCGTTGTTAATGTCATTAACAAGCTTGACGAACCTTTCCTCATCACCTGGAACGGAATTAAACAGAGGAAGATGT CGTGGCAAGACGGTGTTTTGGGAACAAACTGTCCGATCCAACCAAAATCGAGTTGGACTTACCATTTTCAACTTAAGGAC CAAATCGGTACTTATGCATACTTTGCTTCAACGTCGATGCATAGAGCAAGTGGTGCTTTTGGTGCACTTAACGTTAACCA GAGGTCAGTCATCTTCGTTCCTTACCCTAAACCAGACGCTGACTTCACTCTCCTCGTCAGTGATTGGTACAAGATGGGAC ATAAGGAACTGCAAAGGAGGCTCGACTCAAGTCGTGCTCTTCCTCCTCCGGATGGTCTTCTCATCAACGGTGCTTCTAAA GGTTTAGTCTTTACCGGTCAACATGGCAAGATATATAGGTTTCGGATATCGAATGTTGGGATATCGACATCGATTAACTT TAGGATACAAGGACACATGATGACTCTCGTCGAAGTAGAAGGTTCTCACACTCTGCAAGAGGTCTATGAATCGCTTGACA TACACGTTGGCCAGTCTGTGACCGTGTTGGTCACATTGAAGGCTCCAGTGAAGGACTACTTCATCGTGGCTTCAACTCGG TTTACCAAACCTATACTAACCACCACAGGAATCCTCAGCTACCAAGGTTCCAAAATCAGACCATCACATCCCCTTCCCAT TGGTCCTACTTACCATATCCATTGGTCCATGAAACAAGCAAGAACCATCAGGCTAAATTTGACGGCGAATGCAGCAAGGC CAAATCCACAAGGATCCTTTCACTACGGTACTATACCGATAAATCGAACTTTTGTTTTAGCCAACGGAAGAGCCATGATC AATGGGAAACTTCGGTACACAGTGAACCGTGTGTCCTACGTTAACCCAGCAACTCCATTGAAATTAGCCGACTGGTTCAA CATTCCCGGGGTGTTCAATTTCAAAACCATTATGAACATTCCCACACCAGGACCCTCCATTCTCGGAACTTCGGTCTTTG ATGTGGCTCTCCATGAGTACGTCGAATTCGTGTTCCAGAACAACGAGGGATCTATACAATCTTGGCACCTTGACGGCACT AGTGCATATGTTGTTGGATACGGGTCGGGCACATGGAACATGGCTAAGAGGAGAGGTTATAATTTGGTTGATGCTGTTTC AAGGCACACTTTTCAGGTTTATCCAATGTCGTGGACGAGCATATTGGTATCATTAGACAATAAGGGAATGTGGAATTTGA GGTCACAAATATGGTCAAGGAGATACTTAGGACAAGAACTCTATGTTCGTGTATGGAACAACGAGAAGTCTCTTTACACT GAATCCGAGCCTCCTGTTAATGTTCTCTTTTGCGGCAAGGCCAAACATCCCCGCTTGATTTAGATATATTTTTATCAATA AATTCCCTTATCGTGATATGGTTATTATATTTATAGGTTATTACAGTATTAATTAAGTCACGAGGGTGTGACGTTAACGT TG .....N...N.........................................N...N...N..............N..... ................................................................................ ......................N..............N.......................................... .....................................................................N.......... .....................................N.......................................... ..............N.............................................................i... ................................................................................ .............i......................i........................................... .........................................................................i...... ...................................................N............................ ......................N.............................N........................... ................i..i.............................................N.............. ................................................................................ ................................................................................ ............................i.......................................N........... ..........................................................................N..... ...............................i................................................ N...........N................................................................... .......N........................N......i..............................N......... .........................N.........................................N............ ..........N...............................N.........N........................... ...................N............................................................ .................N.............................................................. .. Pos Score Pred ------------------------ 6 0.252 - 10 0.191 - 52 0.260 - 56 0.389 - 60 0.314 - 75 0.182 - 183 0.236 - 198 0.210 - 310 0.265 - 358 0.223 - 415 0.213 - 477 0.729 Yes 574 0.521 Yes 597 0.574 Yes 714 0.779 Yes 772 0.181 - 823 0.216 - 853 0.310 - 897 0.529 Yes 900 0.571 Yes 946 0.112 - 1149 0.507 Yes 1189 0.389 - 1275 0.453 - 1282 0.502 Yes 1392 0.732 Yes 1441 0.418 - 1453 0.432 - 1528 0.160 - 1553 0.201 - 1560 0.651 Yes 1591 0.227 - 1626 0.172 - 1668 0.321 - 1691 0.250 - 1723 0.367 - 1733 0.096 - 1780 0.190 - 1858 0.051 -

Explain the output -- Processing locus 204: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATACATCGTGGGGATCTGAATTCTGCTCAAAATACTTTTGAAACCATTATATTTTACATAATGCGACATATATTAAT TTATGATTTTTGATTAGTTGAGCAAAAAGAGCTGTCGTATATCATATAGTTTTAATGAATCACTCAAAAATTGGCACAAT AGAAACAGTAAAAGAATTATTGAATTTAGAGGATAAAAAAGACTATAATATCGACCGTCCGATATATCCCAGAAAGATTT TAACAGCAACTCTTTGTCTCTTCTTCTTCTTCTTCTTCTTCTTCTCTCCGATTTGCTCAGTCGATTGCGTCATGTCGTCG TTTGATCCGCCGAATCACCGATATTCAGCCTTAGTACTTATTCTTCTCCTGCTTGGTTTTTCGGTAGCGGCGGCCGACGA TGTATCGTGGACCGAAGATTCCAGCCTCGAGTCTCCTGGCTGTACCAATAAGTTTCAAATGGTAAAGGTCTTAAATTGGG TTGATGGTGTTGAAGGAGACTTCCTTACTGGCTTAACTGCTCAATTTGGAGCTGCCTTGCCTTCTGTTCCTGATCAAGCT CTTAGATTTCCTGCTGCTTTTGTAGATCCTTTGGACTCTTGTTCCCATCTCTCTTCCAGGTTAGATGGACATATTGCCTT GTCGATCCGTGGCAATTGTGCTTTCACTGAGAAGGCCAAACATGCTGAGGCAGCTGGTGCTTCTGCTCTCTTAGTCATTA ATGACAAAGAAGATCTTGATGAGATGGGATGTATGGAAAAGGACACATCTCTAAATGTGAGCATACCTGTCTTAATGATC TCTAAGTCAAGCGGAGATGCTCTCAACAAGTCTATGGTTGACAATAAGAATGTTGAGCTTTTGTTGTATGCTCCAAAGCG TCCTGCCGTGGACCTCACAGCTGGATTGTTATTGCTCATGGCTGTGGGAACTGTTGTCGTTGCATCACTGTGGTCAGAGC TTACAGATCCTGACCAAGCTAATGAATCTTACAGCATACTAGCAAAGGATGTTTCTAGTGCTGGGACCAGAAAAGATGAC CCAGAGAAGGAAATCCTTGATATAAGTGTCACTGGTGCTGTATTTTTTATTGTAACAGCCTCCATATTTCTGCTGCTCCT TTTCTACTTCATGTCATCATGGTTTGTATGGGTGCTCACCATATTTTTCTGCATTGGCGGCATGCAGGGTATGCACAACA TCATTATGGCTGTTATATTGAGAAAATGCAGACATCTTGCTCGGAAATCTGTGAAGCTCCCTTTGCTTGGAACAATGTCA GTGTTGTCGCTTTTGGTGAATATTGTTTGTTTGGCGTTTGCTGTTTTCTGGTTTATAAAACGTCACACATCGTATTCTTG GGTTGGCCAAGACATTTTGGGCATATGTTTGATGATCACTGCCTTGCAAGTGGTTCGATTACCTAACATCAAGGTTGCGA CTGTACTTCTTTGCTGCGCATTTGTCTATGACATTTTCTGGGTCTTCATATCTCCATTGATATTTCACGAGAGTGTTATG ATTGTGGTTGCCCAAGGAGACAGCAGCACTGGGGAGTCCATTCCGATGCTACTAAGGATTCCTCGGTTCTTTGATCCTTG GGGTGGTTATGACATGATTGGTTTTGGGGACATCCTCTTCCCCGGTTTGCTCATTTCCTTTGCTTCCAGATATGACAAGA TAAAAAAGAGGGTAATATCAAATGGGTACTTCCTCTGGTTGACTATCGGCTATGGAATAGGTCTATTACTGACATACCTA GGTCTGTATCTTATGGACGGACATGGCCAGCCGGCACTATTGTACATCGTACCCTGCACACTCGGTTTGGCCGTCATACT GGGGTTGGTAAGAGGAGAGCTTAAAGAACTATGGAATTACGGTATCGAAGAATCAGAGTCTCACACACCGGAGGATCCAA TGCCTGTGGCATAATAACATGATTCAACAATATTCTAATGATGTGTTATCAATCAAACTGAATTAGCTTTTCCCCAAAAC ATCGAGCATAGCTTAAGCAGATTTCATATGTGA ...............................................................N................ ..N...................................................N......................... ................................................................................ .......................................................................i........ ...............................................................................i ..........................................................i..................... ...N............................................................................ ................................................................N............... .........................................N...................................... N.................N....i....i...i.....................N...................i..... ................N................i...............N.................N............ .....................................i.......................................... .....................N..........................N..........................N.... ................................................................................ ..........N.......i........i.................................N........N......... .....N...................N................................................N..... ................................................................................ ........................N......N................................................ ...........................N.................................................N.. .............................................N.................................. ........N....N.........................................................N........ .....................N.............................i............................ ............N.........i......................................................... ..............................N................................................N ..................N..................N..N....................................... ...........................N..... Pos Score Pred ------------------------ 64 0.308 - 83 0.189 - 135 0.410 - 312 0.799 Yes 400 0.598 Yes 459 0.691 Yes 484 0.119 - 625 0.193 - 682 0.289 - 721 0.079 - 739 0.092 - 744 0.634 Yes 749 0.530 Yes 753 0.528 Yes 775 0.198 - 795 0.567 Yes 817 0.254 - 834 0.615 Yes 850 0.211 - 868 0.084 - 918 0.677 Yes 982 0.270 - 1009 0.122 - 1036 0.225 - 1131 0.174 - 1139 0.711 Yes 1148 0.536 Yes 1182 0.498 - 1191 0.230 - 1206 0.342 - 1226 0.412 - 1275 0.458 - 1385 0.397 - 1392 0.294 - 1468 0.145 - 1518 0.276 - 1566 0.216 - 1609 0.119 - 1614 0.491 - 1672 0.260 - 1702 0.477 - 1732 0.681 Yes 1773 0.245 - 1783 0.551 Yes 1871 0.296 - 1920 0.466 - 1939 0.251 - 1958 0.069 - 1961 0.096 - 2028 0.040 -

Explain the output -- Processing locus 205: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACAGAGTTTCAAAGAAAATTCGATCTTCTCTGTTTCGTTTCAGATTTTGCTACTTGCTTTTAAAAGGCCCAAATACGAG GAGGCCCATATTAAGTTTTGATTAACAAAACGTAGTCGTTTTATGCATCGTCGTCCTTATCCACGCGTAACGGTTCGCGT TTCTACACAGAGTCAAATATTTCTGCACGGAAGCTTCGAAAAGAGGTCATCAATGGAGTCTTCACTCTTTTCTCCATCTT CCTCTTCTTACTCTTCTCTCTTCACTGCAAAACCTACGCGTCTTTTATCACCAAAACCCAAATTCACATTCTCCATCAGA TCCTCCATTGAGAAACCCAAACCCAAACTCGAGACCAATTCATCGAAATCCCAATCATGGGTCAGTCCCGATTGGCTCAC AACACTCACTCGTACCCTTTCCTCAGGAAAAAACGACGAGTCAGGTATACCAATCGCGAACGCGAAGCTCGACGATGTCG CTGATCTCCTCGGAGGTGCTCTCTTCTTACCTCTCTACAAATGGATGAATGAGTACGGACCCATTTACCGTCTCGCTGCT GGTCCTCGTAATTTCGTAATTGTGAGCGACCCAGCGATAGCTAAACATGTTTTGAGGAATTATCCAAAGTACGCTAAAGG CTTAGTCGCTGAAGTCTCTGAATTTCTATTTGGTTCGGGTTTCGCTATCGCTGAAGGACCTCTTTGGACAGCGAGGCGTA GAGCGGTGGTTCCATCGCTTCACAGGAGGTATTTGTCTGTGATTGTGGAGAGAGTATTCTGCAAATGTGCAGAGAGGCTT GTTGAGAAGTTGCAGCCTTATGCAGAAGACGGAAGTGCTGTGAATATGGAAGCGAAGTTCTCTCAGATGACACTTGATGT CATTGGGTTGTCTCTTTTTAACTACAATTTCGATTCTTTGACTACTGATAGTCCTGTCATTGAAGCTGTTTACACTGCTC TTAAAGAAGCTGAGCTTCGTTCTACTGATCTTCTGCCATATTGGAAGATCGATGCATTGTGTAAGATAGTCCCGAGACAG GTGAAAGCTGAAAAGGCTGTAACTTTGATAAGGGAAACTGTTGAAGACCTTATTGCTAAGTGTAAAGAAATTGTCGAAAG AGAAGGCGAAAGAATCAATGATGAGGAGTATGTAAATGATGCTGACCCAAGTATCCTGCGTTTCTTGCTTGCAAGCAGAG AAGAGGTATCAAGTGTGCAGTTACGGGATGATCTTCTCTCAATGCTCGTAGCGGGTCATGAAACCACTGGATCTGTCCTC ACTTGGACACTTTATCTCCTAAGTAAGAACTCATCTGCATTAAGGAAAGCACAAGAAGAAGTAGACAGAGTGTTAGAAGG AAGAAACCCGGCTTTCGAGGATATAAAGGAGTTGAAGTACATCACTCGTTGTATAAACGAGTCAATGCGTCTCTATCCTC ATCCTCCTGTCTTGATAAGAAGAGCTCAAGTTCCTGACATTCTTCCTGGGAACTATAAGGTCAATACCGGACAAGACATT ATGATTTCAGTCTATAACATCCATCGTTCTTCCGAGGTATGGGAAAAAGCTGAGGAATTTCTGCCTGAACGATTCGACAT AGATGGCGCAATCCCTAACGAAACAAACACTGATTTCAAATTCATCCCATTCAGTGGAGGGCCTAGAAAATGTGTAGGCG ATCAGTTTGCATTGATGGAGGCAATTGTGGCACTCGCGGTGTTTCTTCAGCGGTTAAACGTTGAGCTGGTTCCTGATCAG ACCATTAGCATGACCACAGGAGCAACCATACACACCACCAATGGATTGTATATGAAGGTGAGCCAAAGGTAAAAACCAGA ATTTATGTTTTCATGATAATTGATTGGTGTGAATGGACTTGTTTCATAGTACTCTTGAGAAATAACCACAAAAAAATGAA TTATGGAAAAT ................................................................................ ..........................................N..................................... ....................................................i........................... ................................................................................ ........................................................i....................... ..........................................................................N..... ........................................N...i...N............................... ..............................................N................................. ................................................................................ ................................................................N............... ...................N.........................i....................i.........N... ................................................................................ ...................................................N............................ ................................................................................ .................N..N........N.....N..N......................................... ...........................N.............N...............N...................... ................................................................................ ................................................................N............... ................................................................................ i.....................................N......................................... ..i..................................................................i.......... ..............i................................................................. .........N..............................i..........N............................ ....N.......N...................N..........................................N.... ..N........ Pos Score Pred ------------------------ 123 0.215 - 213 0.694 Yes 377 0.589 Yes 475 0.446 - 521 0.403 - 525 0.732 Yes 529 0.478 - 607 0.329 - 785 0.378 - 820 0.110 - 846 0.695 Yes 867 0.525 Yes 877 0.198 - 1012 0.078 - 1138 0.283 - 1141 0.414 - 1150 0.117 - 1156 0.171 - 1159 0.117 - 1228 0.130 - 1242 0.411 - 1258 0.190 - 1425 0.453 - 1521 0.867 Yes 1559 0.358 - 1603 0.523 Yes 1670 0.703 Yes 1695 0.868 Yes 1770 0.326 - 1801 0.649 Yes 1812 0.326 - 1845 0.115 - 1853 0.081 - 1873 0.076 - 1916 0.084 - 1923 0.088 -

Explain the output -- Processing locus 206: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTTGGCTAATATTTATACAAAATTATCTCACCTGCATTCATATCCACTTAATTAATTAATCATATTAAAGAAAATAAAA CTCAATTAATTAAAAGAATAATTACGAATTTCCATGCGTCACCCAAAAACACTCCCAAAATCCTCAAAATTCGACAATTC CTCTCTCAAGTCTCTCTCAACACCTTCCTTTCAGTATCAAAGAGATTCTCGCTTTTGTCTAACTTTATCGTCTCTCTTCC TTTTTGCCTCCGATTGGCTGATTTTTTCTATGGCCGCTTCTTACCGAAGCACCGACTGGTTTCCTCCTACTACTACTACT GGTTCATTTACGGGCCTAAATACTGACCAGAGTGTTCTCCAAGATCAGGACATAGTTAGCAGTCGCCCTTTTTGTGGACA GGGCACAGAATCATTTCACATTGGAGCTGATACTCCAAGGTCGAACCACTCACACTCGGATGTTCCTAGCTTTGACAAAG TCTCTCCTGGCATGATAAATGAAATGTTTTACCATAGTAATGGTGTACCATCAGATTTTCAGAGGTCATCTCTTTCTACC AGAAATCAATCATCACTGCAGCATTATGGCGACCTCTATGCTGATGATTCAAGATATTTCGTTCCTTTTCACTTATCAAA TCAGCAGTATCCAACCCCTGAAGATATTCATCTTTCTCCGGAGTTTCACATGTTTCAGGCGAACTCTAGACATTTTGATA GGCATGTGAATGAGAGAAACAGTAGTGAGATTGATAATATGATGCGCGCTAGGGGAAATTCTGTCTTAAGAGATAATATA TATGGAGAACCTAGGGGTATGAATCTCAACCCCTTCAGTGGAGAAAACATGTTTCCTCCATTGGCATCTACCAGACCATG TATGAGACACCTTGGTTCAGCAGAATTGCTGGCAAATGATTTTAATATGGGTCCAGCACATGGAGGTTTGCATGATGCTT TTGAATCTGCTGCAAACTTAAGTTACAGAGAGCAAGCATATGCTCAATGCAAAAGAAGCCCTTTCTCTGCTTCTTCTTCT AGCCCAACTTGGGAGAATGATTATAACTTGCCACCACTTGATGAAGCAAGAAGTGAAAGCTACAATGACTTCAGCCACTG CCCTGCCATGCTTGATATGCTTACCGAGAGTAACAGAGGTCCGAGAGCATCCAGGCTAAACAGTAAAAGTAAAATGATCT CTTATGATCGCGTTGATCGTTTTTGTCAGCAAGAATTGCTCTCCCAATTCAGGGACGCAAAGTTCTTCGTTATTAAGTCA TATAGCGAGGATAATGTCCACAAGAGCATAAAGCATTGTGTTTGGGCAAGCACTAAAAATGGAAACAAAAAGTTGGATGC TGCCTATCGTGAAGCAAAGAAAAAGGACGTGGCATGCCCAGTCTTTCTCTTGTTTTCGGTAAATGCCAGTTCACAGTTTT GTGGAGTTGCTGAGATGGTTGGACCGGTTGACTTCAACACAAGTGTAGAGTACTGGCAACAGGATAGATGGTCAGGACAC TTCCCGGTCCAATGGCTTATTGTCAAAGATGTTCCTAACAGTCTCTTTCGTCACATCATAATTGAAAGCAATGACAATAA GCCTGTCACTAACAGTAGAGACACCCAAGAGGTGGGACTGGAAAAAGGTATAGAGATGTTAGATATATTCATCAGCTGTG AGATGCGTTCTTCGATCCTCGACGACTTTAACTTCTACGAAGAGCGACAGATAGCTATCCAAGACAGAAAAGCCAGACAA CGTGCTGTTCTTGAATCGCTCGCACTTTCTGCAACCTCTGCTCCAACATATTCTCTCCACGACGATTTTGTAAGGGAAAT GTCCAAGCACTTTGCAGAGGCTTTGGCCTTGCAACACAGACCCAAATAGACCTCTTCTCTTTTAGGACTTGGGATTGGCT GAGGTTTAATTATGGTTTTTGGATTTTGTACATTTGATAACGTACTTAAGGAGCATAGGTTGTAAGAT ................................................................................ .................................N.............................................. ................................................................................ .............................i.................................................. ................................................................................ ...........................................................N.................... ...........i......N....i...............N........................................ .........................N...........N.....N.................................... .................................................i.............................. ...N.....N............................i..i...................................... .......................................i......N................................. ................N.......................N.......................i............... .......N........i........................................................N...... ...N............................................................................ .............i............................................i.................N... .................................i............................N................. ..............N....................................................i............ ...........N................N.........................................N......... .......................................................i........................ ..N............................................................................. ..............................................................................N. ................................................................................ ...........N........................................................ Pos Score Pred ------------------------ 114 0.266 - 270 0.779 Yes 460 0.181 - 492 0.537 Yes 499 0.497 - 504 0.515 Yes 520 0.340 - 586 0.212 - 598 0.316 - 604 0.293 - 690 0.670 Yes 724 0.303 - 730 0.127 - 759 0.723 Yes 762 0.524 Yes 802 0.530 Yes 819 0.385 - 849 0.442 - 878 0.174 - 882 0.226 - 916 0.343 - 927 0.487 - 940 0.383 - 952 0.374 - 955 0.289 - 1000 0.545 Yes 1007 0.126 - 1057 0.419 - 1081 0.262 - 1105 0.620 Yes 1128 0.369 - 1137 0.516 Yes 1194 0.493 - 1204 0.415 - 1294 0.663 Yes 1339 0.583 Yes 1357 0.140 - 1394 0.592 Yes 1423 0.445 - 1455 0.352 - 1508 0.501 Yes 1532 0.287 - 1549 0.382 - 1591 0.208 - 1656 0.564 Yes 1683 0.328 - 1839 0.378 - 1932 0.104 -

Explain the output -- Processing locus 207: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTATGAAATGTATTAAAGCAAAAAAGACTAATGTTAAAACTTAAAAGATTAAAAATAAAATAAAAAGAAGTTAAAATGAC ATTTAAATAACGTAAAAATAAACGACGTCGTTTAGCCAAAGTTTCTACTATCTATGTATGTGGTGGAACGAGGTCCAATG GTTGAACAAGCAACCTTATGATATATTTTATGAGAAGAGGCAAACAACACCTAACATTGGATTCGAGCTCTCTAGTGAAG AATCCGCCATGGCTGTTCCATTTCTAAGCTCTTCTCTACAACTCACACCCACTTCTCCGATTCTCTTCACTAAAGTAACA CCAACACCAATAATCCACAACCACAGATCAACATGCACTATACCCACAAAGCCTCGTCTACGTCTCCTCCGCCGCTCCGC CGTCGCTGGAACCGCCGTATCTGATCAAACAGAAGGAGGTGGAGATGTTCTTTTAAACCCTGAAGAAGAGAAACGTGTTG AGGTGGCGGATTATGATTGGACAGAGGAATGGTATCCATTGTACTTAACTAAAAACGTCCCTGAAGATGCGCCTCTTGGC CTCACCGTCTACGATCGTCAAATTGTTTTGTATAAAGACGGTGAAGGAACACTTCGTTGTTACGAAGATCGGTGTCCTCA TCGGTTGGCTAAGTTGTCTGAAGGACAATTGATTGATGGGAGATTGGAATGTTTGTATCATGGTTGGCAATTTGAAGGAG AGGGCAAATGTGTCAAGATTCCTCAGCTTCCTGCAAGTGCTAAGATTCCAAAGGCTGCTTGTGTGAAGACGTATGAGGTG AAGGATTCACAAGGAGTTGTGTGGGTGTGGATGTCAACAAAGACACCTCCAAACCCTGAGAAGCTTCCTTGGTTTGAGAA TTTCGCTAGACCCGGTTTTTTCGACATTTCGACTACTCATGAGCTTCCTTATGACCATTCCATTCTTTTAGAGAATCTGA TGGATCCTGCTCATGTTCCTATCTCTCATGATAGAACGGATTTCACTGCTAAAAGAGAAGATGCTCAGCCTTTGGTTTTC GAGGTCACAGAGCGAAGTAATCGGGGTTTCGCAGGGACTTGGGGCCGAGAGAAAGAAGGTGGGAAAGGGAGTAATTTACT TCGGTTTGATGCTCCTTGTGTTCTGCAGAACAATCGAGAGTTCGAGGGGAAGGATGGAGTGAAGAACTATTTTTCAGGGC TGTTTCTTTGTAGACCTACAGGGCAAGGGAAGTCTATGCTTATTGTTAGGTTTGGGGTCACAAAAAGATCGCCTTTGGTT TCGGTGTTACCTCAATGGTTCTGGCATCAGAATGCCTGCAAGGTTTTTGAACAAGACATGGGGTTTCTATCGTCTCAAAA TGAGGTTCTGATGAAGGAGAAAGTACCAACTAAGGACTTGTATTTGAATCTAAAGTCATCGGACACATGGGTCGCTGAAT ATAGAAAGTGGATGGACAAAGTTGGTCATGGGATGCCTTACCATTTCGGGCATAGGACCATATCTCTCCCCAAAGTCCCT CCTGTCGTGGAACATGCCCCGGCAGGGCTCATTGCAGCTCTCTCTGCTTCTTACCCTGCAAAAGGCGGAATTGGGACTAT GCATGCTCCCAATTTGGCTAACCGGTACTTCAGACATATTATTCATTGTAGAAGCTGCAGCAATGTGATCAAATCTTTTG AGCTCTGGAAAAATATCCTCTCTGCCACGGCAGTGGCTTTGACGGCTTTGGCCATTCTGGTGGTTAGTAGACAGTGGAAG GCTGTCCTGTTAGGTTCAGCAGCATTGTGCTCAGCCGCAGCTTATACCTGCTTAAGAGCTATAAATCTAAACACCAATAA CTTCATTAGAACACACAGAAGACTATGAAAATGTATAGTGTCATGTTTCCTCTTCTTCCAACAATATGTATTTCATGAAA TAAATTTTTGCTTGTAAACGCAATTATATAATTCAGAAGGTTCCAAT ..N....N......................N............................................i.... .....................................................N...N...................i.. .................N...........N.................................................. ........i....................................................................... ................................N............................................... ............................................N................................... ............N...............N.....................................N............. ................................................................................ ...................................N............N..........N.................... .......N................................................................N....... ..............................N................................................. ......................................N...........N............................i ............N..............N................................N................... ................................................................................ ........N............................................N.......................... ...................................N............................................ ..............N................N.........................i.....................N ..........N.......................................................N............. ...........i...............N....i............................................... .............N................................................................i. ..N...........................................................N................. ................................................................................ ................................................................................ ........................N.....N...........N......................N........N..... ............................................... Pos Score Pred ------------------------ 3 0.422 - 8 0.362 - 31 0.265 - 76 0.564 Yes 134 0.146 - 138 0.402 - 158 0.628 Yes 178 0.132 - 190 0.245 - 249 0.696 Yes 353 0.337 - 445 0.438 - 493 0.192 - 509 0.328 - 547 0.423 - 676 0.233 - 689 0.289 - 700 0.256 - 728 0.498 - 793 0.281 - 831 0.204 - 919 0.144 - 931 0.190 - 960 0.823 Yes 973 0.218 - 988 0.138 - 1021 0.404 - 1129 0.065 - 1174 0.321 - 1236 0.219 - 1295 0.150 - 1312 0.167 - 1338 0.611 Yes 1360 0.337 - 1371 0.366 - 1427 0.312 - 1452 0.632 Yes 1468 0.217 - 1473 0.687 Yes 1534 0.305 - 1599 0.634 Yes 1603 0.303 - 1663 0.208 - 1865 0.282 - 1871 0.353 - 1883 0.083 - 1906 0.090 - 1915 0.039 -

Explain the output -- Processing locus 208: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTATTCTCTTACTCTTCCTATGTAACAAATGAGGAAAGTAAAAGGGAGAGATCAGATTTGATTTCTTTTTCCATTTTTG TTTAGATCTTAGGGTTTCTGAATAATGTATCCCTCCAGAAGAAGATTATCTCTTTCCAGAGCCATTGATCAACAACCGGA AGATCAATCAGGAGTCCAAGAAAAGAGTTGCAGATTCTTAGGAGCAATGAGAATCACAGAGATCTTAGCTTTGTTCATGG TTTTAGTCCCTGTCTCGCTAGTAATCGTAGCCATGTTTGGATATGATCAAGGAAATGGCTTTGTACAAGCATCTAGATTC ATAACAATGGAACCAAATGTGACATCCTCATCAGATGATTCATCACTAGTGCAGAGAGATCAAGAACAAAAAGATTCTGT AGATATGTCTCTGCTTGGAGGGCTACTTGTATCTGGTTTCAAGAAAGAGTCTTGCTTGAGTAGATACCAATCTTACCTCT ACCGTAAAGCTTCACCGTATAAACCTTCGTTGCATCTACTTTCGAAGCTTAGAGCTTACGAAGAGCTTCATAAAAGATGT GGACCGGGAACAAGACAGTATACCAATGCAGAAAGATTGCTTAAACAGAAACAAACAGGTGAGATGGAATCACAAGGATG CAAGTATGTTGTTTGGATGTCGTTTAGCGGATTAGGAAACAGGATTATCAGTATTGCTTCTGTGTTTCTGTATGCAATGT TGACAGATAGAGTCTTGCTTGTTGAAGGAGGGGAACAGTTCGCGGATTTATTCTGCGAACCGTTCCTCGATACCACTTGG TTACTACCGAAAGATTTCACCTTAGCTAGTCAGTTCAGTGGCTTTGGTCAAAACTCAGCTCACTGCCATGGAGATATGCT GAAGAGGAAACTGATTAATGAATCCTCTGTTTCGTCTCTGTCTCATCTCTATCTTCATCTAGCTCATGACTACAATGAGC ACGACAAAATGTTCTTCTGTGAAGAAGATCAAAATCTCTTAAAGAATGTTCCTTGGTTGATCATGAGGACAAACAACTTC TTTGCACCGTCTCTTTTCTTGATTTCTTCTTTCGAAGAAGAGCTCGGTATGATGTTTCCCGAGAAAGGAACGGTTTTTCA CCATTTAGGTCGTTACCTTTTCCATCCTTCAAATCAAGTCTGGGGACTAATCACAAGATACTATCAAGCTTACTTAGCCA AAGCTGATGAAAGGATTGGTCTTCAAATAAGAGTCTTTGATGAGAAATCCGGCGTATCTCCTCGAGTCACAAAGCAAATC ATTTCGTGTGTTCAAAACGAGAATCTGTTACCGAGACTAAGCAAAGGTGAAGAACAATACAAGCAGCCATCAGAAGAAGA GTTGAAACTCAAATCTGTCTTGGTCACCTCTTTAACAACAGGATACTTTGAGATCTTGAAAACAATGTATTGGGAAAATC CAACTGTAACAAGAGATGTGATTGGAATACATCAGCCAAGTCATGAAGGACATCAACAAACAGAGAAGCTAATGCATAAC AGGAAAGCTTGGGCAGAGATGTACTTACTCAGCTTAACGGATAAGTTGGTTATTAGTGCTTGGTCTACATTTGGTTATGT AGCTCAAGGACTTGGAGGATTAAGAGCTTGGATTCTGTATAAACAAGAGAATCAAACCAACCCAAATCCACCTTGCGGTA GAGCTATGTCACCAGATCCTTGTTTCCATGCTCCTCCTTACTATGATTGCAAAGCAAAGAAAGGAACTGACACTGGTAAT GTTGTCCCGCATGTTAGACATTGTGAAGATATTAGCTGGGGACTTAAGCTTGTTGACAACTTTTAGATAATCAACATTGT AATGTTTCCTTTTTTTCTGAATGTTTCAGTTGATATTAAAAGATAGAAGAACCAAGCCATTTCCATTATTGTCTTTCACT AATGA ....................N........N.................................................. ........................i....................................................... ..............................................i.............................N... ................................N.........N...........i......................... ......i.........N.................N............................................. ....N........................................................................... ............................................................................N... .........................N.....................................N.............N.. .....N..........N......................................................N....N... ................................................................................ ...................................................................i.......i.... .................N...............................................N........N..... ........N....................................N................i................. ................................................N..i............................ ................................................................................ ......N................................N........................................ ................................................................................ ................................................................i............... ...............i..........................N............................N........ ..................N.........................................................N... ................................................................................ .....i.....................N..............N...................................N. ..........N..................................................................... Pos Score Pred ------------------------ 21 0.232 - 30 0.263 - 105 0.805 Yes 207 0.524 Yes 237 0.300 - 273 0.373 - 283 0.367 - 295 0.629 Yes 327 0.530 Yes 337 0.219 - 355 0.171 - 405 0.494 - 557 0.223 - 586 0.332 - 624 0.392 - 638 0.355 - 646 0.391 - 657 0.451 - 712 0.221 - 717 0.288 - 868 0.570 Yes 876 0.703 Yes 898 0.313 - 946 0.247 - 955 0.241 - 969 0.203 - 1006 0.325 - 1023 0.548 Yes 1089 0.239 - 1092 0.680 Yes 1207 0.137 - 1240 0.374 - 1425 0.735 Yes 1456 0.563 Yes 1483 0.157 - 1512 0.395 - 1539 0.310 - 1597 0.163 - 1686 0.586 Yes 1708 0.197 - 1723 0.307 - 1759 0.095 - 1771 0.092 - 1842 0.133 - 1861 0.050 - 1922 0.038 -

Explain the output -- Processing locus 209: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACAATAGAAATGTAGTTAGATTCTTCGACACCATCTTCGTTTTCGATTGGATCTACATGTTATGCTCTCTCTTTTTCCT TGGAATACTATGTCAGATTGAGTAATGGTTGTCCTTGTATTGAATTACAGAAGAAAACCAAGCAGTAGTAGAATGGATCA TTCAGCGAAAACCACACAGAACCGTGTTTTGTCAGTGAAGATGTGGCCACCGAGTAAGAGTACCCGTCTCATGCTTGTTG AGCGGATGACCAAGAACATTACCACCCCTTCCATCTTCTCCAGGAAGTACGGTCTTTTGTCTGTTGAAGAGGCTGAGCAA GACGCCAAGCGCATTGAAGATTTGGCCTTTGCTACTGCCAACAAACACTTCCAGAACGAGCCTGATGGTGATGGCACTTC TGCTGTTCACGTCTATGCTAAAGAATCCAGCAAGCTCATGCTTGATGTCATCAAACGTGGTCCACAGGAAGAATCCGAGG TTGAGGTGAGCAAGGATGGTGATGTCTTTTTTGATATATCTGGTGGGAGCAGAGCTTTTATTGAAGAAGAGGAGGCACGC GACCTTTTGAGGCCTCTGGCTGACCCGCGTAACTCCTATACCAAAATCCGTTTTAGCAACAGGAGTTTTGGGTCTGAAGC AGCCAAATTTGCTGCGAGCGTTTTGTCTTCCATCAAGGATCAGCTGACGGAAGTGGATCTCTCGGATTTTGTTGCAGGAA GACCAGAGGCTGAAGCGCTTGAAGTCATGAATATGTTTTCATCTGCTTTAGAGGGTTCTAAGCTTAGGTATCTGAATCTC TCGGACAATGCTTTGGGAGAAAAGGGAATTAGGGCATTTGCTTCTCTCATCAATTCTCAGCATGACTTGGAGGAGCTCTA TCTGATGAATGATGGCATTTCCGAGGATGCTGCACGTGCAGTTCGTGAGCTTCTCCCTTCTACTGACAAAATCAGGGTTC TTCAGTTTCATAACAACATGACAGGAGATGAAGGAGCAACTGCCATTGCAGAGATTGTCAGGGAGTGTCCATCTTTGGAA GATTTCCGGTGTTCATCTACAAGGATTGGTTCAGAAGGTGGTGTTGCACTAGCTGAAGCACTTGAACACTGTAGCCACTT GAAGAAACTTGATCTTCGTGACAACATGTTTGGAGTCGAAGGTGGAATTGCCCTGGCCAAGACTTTATCAGTCTTGACTC ATTTAACCGAGATCTATATGAGTTACCTGAACTTGGAGGATGAGGGAACAGAGGCACTTTCTGAAGCTCTACTTAAGTCC GCCCCTTCTCTTGAAGTTCTAGAATTGGCTGGTAACGACATTACTGTAAAATCCACTGGAAATTTGGCAGCTTGCATCGC CTCCAAACAGTCTCTTGCAAAGCTGAATTTATCTGAGAATGAACTCAAAGACGAAGGGACTATCTTAATTGCAAAGGCAG TTGAAGGTCATGATCAGTTAGTTGAAGTTGACCTGAGCACCAACATGATCAGAAGAGCTGGGGCAAGGGCCTTGGCACAA ACTGTTGTGAAGAAAAATACTTTCAAGCTTCTGAACATCAATGGAAACTTCATATCTGAGGAAGGTATTGACGAGGTAAA TGACATGTTTAAGGACTGTCTGGACAAGCTTGTTCCTTTGGACGACAATGACCCTGAAGGAGAAGATTTTGAAGATGAAG ACGAAGAGGAGGAAGGCGAAGATGGCAATGAATTGGAATCAAAGCTTGGAAGTCTGAAAATCAAGCAAGGGGAGGAATGA TGTGATGCTTTTGTTGCTATGTAGGTAGGTTCAGTGTTATCTCTCTTTCTGGATTCGGTAAACATAGTTAAGTAGGTATC TAAGGATCTAAGATTGTCT ..........i..............................................N....N................. .........N..............N...............................................i....... ........................................N.............................N......... .....i.......................................................................... ................................................................N.....N......... ..............N......................i......N................................... ...............i.....N.......................................................... ................................................................................ ................................................................................ ..........................N.....i............................................... .......N.....................................................N.................. ....i...N..N..............N..................................................... .................i.........i.................................................... ................................................................................ .........................i...................................................... .................i.....................N........................................ ................................................................................ ......................................N......................................... .........N..................................N................................... ........................................N......................................N ....i..........................................N..........................N..... .....................N.....N................................................N..N ....N.............N............................................................. ................... Pos Score Pred ------------------------ 11 0.636 Yes 58 0.269 - 63 0.437 - 90 0.249 - 105 0.463 - 153 0.570 Yes 201 0.460 - 231 0.433 - 246 0.625 Yes 385 0.450 - 391 0.209 - 415 0.098 - 438 0.571 Yes 445 0.156 - 496 0.579 Yes 502 0.250 - 747 0.459 - 753 0.520 Yes 808 0.252 - 862 0.434 - 885 0.689 Yes 889 0.190 - 892 0.375 - 907 0.398 - 978 0.757 Yes 988 0.715 Yes 1146 0.722 Yes 1218 0.558 Yes 1240 0.474 - 1399 0.216 - 1450 0.100 - 1485 0.325 - 1561 0.280 - 1600 0.444 - 1605 0.739 Yes 1648 0.293 - 1675 0.374 - 1702 0.273 - 1708 0.203 - 1757 0.270 - 1760 0.293 - 1765 0.051 - 1779 0.160 -

Explain the output -- Processing locus 210: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCAATTTCAGTTCTGGAAATTGATTCTATGTATCTAGTCTTAGAGATGGTATCTCTTGTATTGAATCATATTGAGATGG TATCACTCACATTCTGTGTAAACTGTTTCATTTTTGCTTTGTGTTTCCTTTTTAGTTGTTTGGGTGGTATGTATGTTTCA CCTACTTGAATTCCCAAATCTAAATCATCCTTTATTGTTTTCACCAATGTCTTGTTTCATTTTGACACAAGGTTTTAAGA ACATGCCTCCTAAAGAGGAGAGTTCGAAAGTCACTCGAAGAAGCACTAGGTCATCAGCTTCTGTAACTGTTGAGAACTCA GAACCTATTGAATCCCACACACCAACCATTGATGATCTCACATTTGGGGAAGAATCAATCACAATTGATGCTGTTCTATC TAATTTTCCAGGCAGACGCTCTCAGATTTTCGACTTTATTCGACTTATGGGGCCGTTAGATTGCCCTACTCTTCCAATAA TGATCTATGGAGGTGCTTCTACGGGAAAAACCAGTGTTGTTCTTCAGGTGCTCAGGCATCTCAATCGTCCCTTTGTTTAT TCAAGCTGTCGTACGTGTTACAATCCCCGTATCTTGTTTGAGTCGATTCTGAATCAGTCGTTGCTACATAGGAAGTGTTC TTTAAACGGGTATTCTAGTGCGAAACGATGTGACAAGCCATCTGATTTTGTCAACTTGCTTCGTGAAGCCCTTAGTAGTG TGATAAAGACTCTCGAGTCTACTTCCGAAACCTCAAGATCAGATAAGCCGGATGAAAAACCCATGGGGAAGATGGTGTAC TTAATACTTGATAATGTGGATCTTATCAGAGACTGGGATAAAGGCACCATCATTCTGCAGTTTCTTTTCAGTTTGTATAC TGTTTTGAAGATGCCTCAACTTGGTATAATACTTATCAGTGGTTTGCCACCAGATGTGTATTACTCAAACATGGGATACA CAGATCCTATACCTCTTTATTTTCCTGAGTATTCTGAAGAGGATCTTCGTCAAATTTTCTTGAGAAATCAACCTAACCGA AAATTGTACTCTGCATTCCTTGACGTTGTGTTGAAACCATTCTGTAGAGTCACCAGGAGAGTTGAGGAACTGTCTACTAC CTTCTCATTATTGTTCAGAAAGTTTTGTGAGCCACTTGATGATTTAGGGATTTCACCAAATGAAGACATGAAGAGAAGGC TATATAGCCATCTTAAGCCGCTTATTGCTCATTGTTTAAATGAGATATTCAGGGTCTCTAGTCACCCTCATGATGGCGAA ACCAGAGGCGAGAGAAGGCAGAAAGCCAGCTACAGTTCTGAAAACCGTGAAGAACTTGAAATATTGGACTTCCATATGTC TACTTCAGCAAAGTTTCTTCTTCTTTCGGCATTTCTCGCTTCGAGAAACCCAGCAACGCTAGACGCGTCAATGTTTGACT CCACAGGGGGAATGGATAATCGTAAAAGAAAGCGGAAGGCCTCTGAGAAATCAATGGAAAAGAAGGAAATTGCAGAGCAG GAAGCAGTCATGAAAGGACCTGGAAGCTTTCCACTGGAAAGACTATTGGCGATATTCCAATGTATTGCCTCCGTTGGAGA TTCTTCATTTGGCGAAGAGGACGAAGAAGAAGAAAACACAACGGGTTACGACAAAGAGAATAACAATCTGATGTCTGATA TTCTGCTGCAAGTGTCAAGTCTATGTGATGCTAACTTTTTAATCAAAAGCGGAAGCTGCCCATTAGAAGGTTCTATTAGG TACCGTTCTATGGTCTCTGAAGATCTGGCTCAGAAGGTTGCAAAAAGTCTTAGCTTTCCACTATCCAAATACTTGTACAG AAGATGATGAAAATGTGAAATTCAAATGTTTTGGGAGGAAGAAACTAACGGTGCACTCTTAATTTAGTTCTTGTATTGTA CTATGGATGTATCGGAAACTTCATAG ............................N.................N.............................N... ....................................................................N...N....... ..............................................i................................. ..i............................................................................. ...............................N...................................N............ ..............................................i................................N ......N......................................................................... ................................................................................ ...........................N.................................................... ...................................................N..........N........N........ .............N.................................................................. ..........N..........................................N................i......... ................................................................................ ................................................................................ ......................................N....................i.......i............ .......................................i.............................N..N....... ...........................................................................i.... ......................................................................N......... ...........i.........................................N.......................... .........i.................................................N.................... ......................................................................N......... ......................N....N.................................................... .........N...................................................................... ...N..N.....N............N...................................................... ..N...N................... Pos Score Pred ------------------------ 29 0.198 - 47 0.358 - 77 0.153 - 149 0.196 - 153 0.193 - 207 0.769 Yes 243 0.736 Yes 352 0.158 - 388 0.287 - 447 0.662 Yes 480 0.253 - 487 0.452 - 668 0.365 - 772 0.112 - 783 0.296 - 792 0.359 - 814 0.215 - 891 0.323 - 934 0.462 - 951 0.833 Yes 1159 0.082 - 1180 0.528 Yes 1188 0.609 Yes 1240 0.771 Yes 1270 0.251 - 1273 0.263 - 1356 0.838 Yes 1431 0.466 - 1452 0.730 Yes 1494 0.417 - 1530 0.597 Yes 1580 0.344 - 1671 0.301 - 1703 0.150 - 1708 0.217 - 1770 0.207 - 1844 0.427 - 1847 0.200 - 1853 0.177 - 1866 0.216 - 1923 0.060 - 1927 0.042 -

Explain the output -- Processing locus 211: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACCGACGGTTTAATTTGGCTAGAAAAATTCAAACAATTATGATGTTGTGCCTTAATGTGACAACATAGATAAAAAGAAGC TCTTACAAACAAACTGACTAAGTCTTCCTCGGAGTCAAAATCATAAGCATATTCCATGCTCATACCAATAAAGTAATGAA AATGACAAAGAGACTTATTAATCGTTATAAAGATCCTTGTTCTGTTTCACTCATCATGAATTCGAAATTTCGATATAACA AACCAAAAGGAAAGAAGCAAAAAAAAAAAAAAAAAGCTCTGGATTCGGAATCTCAGATAAAAAAACCAACGATGCCGATA AAGGTATTGTCGTCGCTTGATGTAGCTCGAACACAATGGTACCATTTCAAAGCGATCATAGTAGCGGGAATGGGTCTATT CACCGACGCATACGATCTTTTCTGCATAGCACCGGTCATGAAAATGATTAGTCATGTTTATTACAACGGTGACTCAATCA ACACAGCCGTTCTTTCAACTTCTTACGCCATCGCTTTACTCGGCACAGCCACGGGTCAACTAGTCTTCGGTTACCTCGGT GACCGAGTCGGTCGTCGCCGAGTCTACGGACTTTGTTTGATTATTATGATCCTTAGCTCCTTTGGTTGTGGCTTTTCCGT GTGCACCACTCGTCGGTCTTGTGTTATGGTTAGCCTCGGTTTTTTCCGGTTCTTTCTTGGTTTAGGTATCGGTGGTGATT ACCCGCTCTCGGCTACCATTATGTCGGAGTTTGCTAATAAGAGGACACGTGGCGCGTTTATCGCTGCGGTGTTTTCGATG CAAGGGTTGGGGATTTTGGTTAGCTCTGCCGTGACCATGGCTGTTTGCGTGGCGTTTAAGAGAAGTGGCGGTGGTTTGGA GGTGGATGCGGCGGCTCCGACGGAAGCTGACTTGGCCTGGAGACTTATACTTATGATCGGTGCTCTTCCTGCTGCATTGA CGTTCTATTGGCGAATGTTGATGCCTGAAACCGCAAGATACACAGCACTTGTAGAAAACAATATCGTCCAAGCAGCAAAA GACATGCAAAGAGTCATGTCAAGATCTCATATCTCCGACGAAGCCACGACGGATCCACCTCCTCCTCCGCCGCCACCTTC CTACAAACTCTTCTCCCGCTGCTTCTTCCGCCTCCATGGTCGCGACCTCTTCGCAGCCTCCTTTAATTGGTTCCTAGTAG ACATTGTCTTCTACACAAGCAACCTCCTCCTGTCCCATATCTTCAGTCATTACTCCAAGAAACCTTCCACTGCAGAAAAC GTCTACGACGCAGCCTTTGAAGTGGCGGAATTAGGAGCCATCATCGCTGCTTGCTCCACCATTCCCGGTTATTGGTTCAC AGTTTACTTCATCGACAAGATAGGTCGTGTCAAAATCCAGATTATGGGGTTTTTCTTCATGGCCGTTATTTATTTAGTCG CTGGTATTCCGTACAGTTGGTATTGGTCAAAGCACGAGCATAATAATAAAGGCTTCATGGTTCTCTACGGTTTGGTTTTC TTCTTTTGCAATTTTGGTCCCAACACTACAACTTTTATTATTCCCGCAGAGCATTTCCCGGCTAGGTTTAGGTCTACTTG TCACGGGATCTCTGGTGCCGCGGGTAAGCTTGGGGCTATTGTGGGCACTGTTGGGTTCTTGTGGGCTACCAAGAAGATGG AAAGCGATGATAAAAACCAGATTTATCCCGAAGTAAACCGTATGAGAATTGCTTTCTTGATTCTTGGTGGCGTTTGCATT GCTGGAATCTTGGTGACGTATTTCTTCACCAAGGAAACTATGGGAAGATCGTTAGAGGAGAACGAGCATGACCAAGATAA CAATGCTGAGTCTGAAGATGAGCCACAGATTGTTGACGGGCAATCCTCCGTGAGCACTTTGCTTCAGACACGATGATGAT CTCGACATATTGAACACATGATCAGTTCAAGTTCATACAATTGTATATTTCTGCTAATCTTTTCTAGATTTTGTTTACAT TTTGCTTTGAGGTAT ......................................N..i............N......................... .......................................................N...................N.... .......................................................................i........ ...................N...............N.................................N.......... .....................................i.....i.........N.......................... ................................................................................ .............................................N.................................. .........................i...................................................... ....................i........................................................i.. ....................................i........................................... .....N..............................................N........................... ..............N.....i........................................................... ...i...........N................................................................ ...................................i............................................ ................................................................................ ................................................................................ ...........................................i..............N..................... ........................................................N....................... ................................................................................ ............................................................................i... ......N..................................i...................................... .......................................i...........................N............ ..i..............N......................................................N..N.... .................N.............................................................. ............... Pos Score Pred ------------------------ 39 0.413 - 42 0.508 Yes 55 0.361 - 136 0.236 - 156 0.453 - 162 0.490 - 216 0.527 Yes 312 0.705 Yes 340 0.162 - 356 0.374 - 390 0.354 - 438 0.508 Yes 444 0.779 Yes 454 0.496 - 606 0.357 - 666 0.765 Yes 741 0.722 Yes 798 0.782 Yes 837 0.666 Yes 886 0.215 - 933 0.278 - 975 0.446 - 981 0.531 Yes 1044 0.651 Yes 1056 0.309 - 1156 0.584 Yes 1404 0.504 Yes 1419 0.439 - 1497 0.462 - 1677 0.593 Yes 1687 0.209 - 1722 0.560 Yes 1800 0.664 Yes 1828 0.278 - 1843 0.513 Yes 1858 0.266 - 1913 0.182 - 1916 0.221 - 1938 0.067 -

Explain the output -- Processing locus 212: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATGGTAAAGAAATAATTAACACTAAACAAGAGTCAAAAGAACATGACGTAAATATTACATCAACTTTACGTATAAAC CCCTTCCCTCTCTCTATTTAAACCCAACACATACATATGAAACTCCTCAACAAACACAAAAACCTCACAACCACTAAACA ACACACAAAAATGGCGTTTGTATTAATGAATAACACAAACGCGTTTCTTGTAACGCTACTATTACTATCCCTTTCTTACA TTCCATTGTCTTTTTCCACAATCCAACAAGACTTCGTGATGTGTCTAGTCGACAACTCCGACGCTTCCTTTCCAATGGAC TCATCGTTCTTCACTCACGACCTAAACGCCTCTTCCTTTAAACTCGCCTTAGAGACGTCAGCTCAAAATCTCCGTTACTT GATGCCTTCAAATCCTAAGCCAGAGTTCATTTTCGAGCCGCTTTACGAAACGCACGTTCAGGCCGCCGTTCTTTGCGCCA AGAAGCTGAAGCTTCACTTGCGGTTAAGAAGCGGTGGTCATGACTACGAAGGCCTCTCTTACGTCTCGGAGATGGAAACG GCGTTTGTGATCGTCGACTTGTCCAAGCTTAGACAGATCAGTGTTGATATAGAAAGTAACAGCGCGTGGGTTCACGCTGG TGCTTCTATTGGAGAGGTTTATTACAGGATCCAAGAGAAAAGCAAAATCCACGGTTTTCCGGCGGGTTTATGCACCAGCC TAGGCATCGGCGGTCACATAATCGGCGGAGCCTATGGTTCAATGATGCGTAAGTTTGGTCTCGGAGCTGATAACGTCCTC GATGCTAGGATCGTTGACGCTGACGGCAAAATCTTGAATCGCGCGGCGATGGGAGAAGACGTTTTCTGGGCGATTCGAGG AGGTGGAGGAGGAAGCTTCGGCGTAATACTTGCCTGGAAAATAAAGCTCGTTCCGGTGCCAGAGATTGTTACGGTGTTTA CCGTCACGAGGACGCTTGAGCAAGACGGAACTAAGCTTTTGTACAAGTGGCAACAAGTCGCTGATAAGCTCGACGAAGAT CTCTTTATCCGTGTGATTATTCAGCCGACGAGCAAAACTCCGAAGAGCAAAGAAAGAACTATCTCGACTTCGTACCAAGG CCAATTTCTCGGCGACGCTAATCGTTTGTTGCAGGTGATGCAAAGGAGTTTCCCACAGCTTGGACTAACGAAGAAAGATT GTTTAGAGACAAGCTGGATCAAATCGGTGATGTACATTGCAGGTTTTCCAAGCACTGCCCCATCAGAAGCTTTACTCGAT GGGAAATCGTTGTTCAAGAATTACTTCAAAGCCAAGTCAGACTATGTGGAAGAGCCAATTCCAGTAGAGGGATTAGAAGG GTTGTGGGAGAAGCTTCTAGAAGAGGACTCACCATTGACTATATGGAATCCTTATGGAGGAATGATGGCAAAAATTCCCG AGACAGAGACACCTTTCCCACATAGGAGTGGGACATTGTTCAAGATACAATGGCTGACTTTGTGGCAAGATGGGAAAACA AGCGAGGCGAAGCATATGGGGTGGATGAGGGAAATGTATAGTTACATGGAGCAGTATGTATCGAAAAGCCCGAGATCCGC GTACGTGAACTATAGAGATCTTGATTTGGGGATGAATGGTAAAGGGAGTGATGCAAGAGAATGGGGGAATAGGTATTTCA AGGGTAATTTTGAAAGGTTGGTGGAGATTAAAGCTAAGTTTGATCCTGAGAATTTCTTTAGACATGAACAAAGTATTCCT ACAGAACTTGAGTGAATTTGGAGTTAGACAAGAATAAGAGTGTGGTTTAAGATTTCTCTTAGGAAAGACTTGTTACTTGT GTTTTTGTGCTTTTAAACATGGCCAACACACTTG ...N.........................................i.................................. ....................................i........................................... ..........i..............N...................................................... ......................................i...................................i..... ................................................................................ .......................................N...............................i........ ................................................................................ .....................................................................N.......... .................................N.......i..i................................... ................................................................................ ................................................................................ ................................................................................ .....................................N.......................................... .............................N................................................N. ...........................................N.................................... ..........................................N..........N.......i..N............... .................................................i...................N.......... ...............N........N........N...........N.........N........................ ...............................i...N..............i.........N................... ...............................................................N................ ................................................................................ ..................N............... Pos Score Pred ------------------------ 4 0.410 - 46 0.553 Yes 117 0.526 Yes 171 0.571 Yes 186 0.376 - 279 0.519 Yes 315 0.602 Yes 402 0.562 Yes 520 0.383 - 552 0.817 Yes 710 0.205 - 754 0.224 - 762 0.748 Yes 765 0.806 Yes 802 0.345 - 849 0.479 - 1158 0.279 - 1230 0.379 - 1279 0.369 - 1324 0.343 - 1403 0.448 - 1414 0.274 - 1422 0.530 Yes 1425 0.322 - 1490 0.832 Yes 1510 0.251 - 1536 0.353 - 1545 0.449 - 1554 0.411 - 1566 0.284 - 1576 0.249 - 1632 0.520 Yes 1636 0.095 - 1651 0.521 Yes 1661 0.403 - 1744 0.064 - 1859 0.087 -

Explain the output -- Processing locus 213: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTGAGATCAATGAAATGAGAAAAATTAAATAGAACTTTTATGATATTTTAATGTTAATTGATACTATATAAAGCAGGG TCTAATTCTATTTGCTTTTAAAACTGCCTAACTTATTTCAAGTTCGGTATAACTAACTACTTTATTGTTTGTAATGAGTT ATTTAAGGGTACTTATATGTCGAACTTTTTTTTGTGTGATGAAAAATTGCAGAGGAGAAGAAGAATGGCAAGGGAACAAT TACAAGTGTTGAATGCACTTGACGTGGCCAAGACGCAATGGTACCATTTCACGGCGATCATAATCGCCGGAATGGGATTC TTCACTGATGCTTACGATCTCTTTTGCATCTCTCTCGTAACGAAGCTCCTCGGTCGTATTTATTACCACGTGGAAGGCGC ACAAAAGCCTGGGACTCTCCCTCCCAACGTCGCAGCCGCCGTCAATGGCGTTGCCTTCTGTGGGACTCTCGCCGGTCAGC TCTTTTTCGGGTGGCTTGGTGATAAGCTCGGGAGGAAGAAAGTTTACGGTATGACGTTGATGGTCATGGTCCTTTGTTCA ATAGCCTCTGGTCTCTCTTTCGGACATGAGCCAAAAGCTGTGATGGCCACGCTCTGTTTTTTTCGGTTTTGGCTTGGATT TGGCATCGGTGGTGACTACCCTTTATCCGCAACCATCATGTCTGAATATGCGAACAAGAAGACTCGCGGAGCCTTTGTCT CTGCGGTTTTTGCTATGCAAGGGTTCGGAATCATGGCTGGTGGTATTTTCGCTATTATAATTTCCTCTGCTTTTGAAGCT AAGTTTCCATCCCCGGCCTATGCGGATGATGCCTTGGGATCCACGATTCCTCAAGCTGATTTGGTATGGCGGATAATCCT GATGGCTGGTGCTATCCCTGCGGCTATGACGTATTACTCAAGGTCGAAGATGCCTGAGACCGCAAGGTACACGGCTTTGG TTGCTAAGGACGCAAAGCAGGCAGCTTCGGACATGTCTAAGGTTCTGCAAGTGGAGATAGAGCCAGAACAACAGAAATTG GAAGAGATCTCAAAGGAGAAGTCCAAGGCCTTTGGATTGTTCTCAAAGGAATTCATGAGTCGCCATGGGCTTCATTTGCT AGGCACTACATCGACATGGTTCCTTCTCGACATTGCTTTCTACAGTCAAAACCTTTTCCAAAAGGATATTTTCAGCGCGA TCGGATGGATTCCTCCCGCGCAAAGCATGAACGCAATTCAAGAGGTTTTCAAGATTGCCCGTGCGCAAACGCTAATCGCC TTGTGTAGCACGGTACCTGGTTACTGGTTCACAGTTGCGTTCATCGACGTCATTGGAAGATTTGCGATTCAGATGATGGG TTTCTTTTTCATGACGGTCTTTATGTTTGCTCTGGCTATTCCTTACAACCACTGGACTCACAAGGAGAACCGAATCGGAT TTGTTATCATGTACTCGTTAACATTCTTTTTCGCCAACTTTGGACCCAATGCTACAACCTTCGTTGTGCCGGCCGAAATC TTCCCAGCCAGGTTCAGATCAACCTGCCACGGTATCTCTGCAGCATCAGGAAAATTAGGAGCAATGGTTGGTGCGTTCGG GTTCTTGTACTTGGCTCAGAACCCAGACAAGGACAAGACCGACGCAGGATACCCTCCAGGGATTGGGGTCAGGAACTCGC TTATTGTGTTGGGTGTAGTTAACTTCTTAGGTATCCTCTTCACTTTCTTGGTACCTGAATCTAAAGGTAAGTCACTCGAG GAAATGTCCGGTGAAAATGAAGACAATGAGAATAGCAACAATGATAGTAGAACGGTCCCAATAGTTTAGGTGATATAATA CGCCTTTTGTAATAATTTTCGTTTTTTCTTTCTCCTTGTCTCTAGCAACTCAAGTTGTTCTTTGTGTAATCCATTGATAC CTAATTAA ...........N....N........................N..........N........................... .........................................................................N...... ................N.....................N.........................i............... ............N........................N.................................N........ .......N........................................................................ ............................................N................................... ..................................................N........N.....N.............. .........................N................i..................................... .....................................i.........N................................ ..............i.................i............................................... ...................N.....N..N....................................i.............. ................................N............................................... ......................................................N.........N............... ...............N................................................................ ....i.....................i..................................................... ........................................................................i..i.... ..........N...........N......................................................... ........i.......................................N............................... ...............................................................i................ ................................................................................ ................................................................................ ...N............N........N..............N....................................... ................................................................................ ........ Pos Score Pred ------------------------ 12 0.231 - 17 0.205 - 42 0.076 - 53 0.105 - 154 0.158 - 177 0.464 - 199 0.189 - 225 0.663 Yes 253 0.094 - 278 0.260 - 312 0.376 - 328 0.374 - 445 0.476 - 531 0.178 - 540 0.270 - 546 0.263 - 586 0.210 - 603 0.790 Yes 678 0.713 Yes 688 0.281 - 735 0.695 Yes 753 0.807 Yes 820 0.140 - 826 0.204 - 829 0.168 - 866 0.729 Yes 882 0.827 Yes 906 0.543 Yes 930 0.859 Yes 993 0.453 - 1095 0.282 - 1105 0.252 - 1136 0.452 - 1205 0.556 Yes 1227 0.717 Yes 1353 0.503 Yes 1356 0.542 Yes 1371 0.181 - 1383 0.302 - 1449 0.739 Yes 1489 0.203 - 1584 0.840 Yes 1764 0.440 - 1777 0.183 - 1786 0.215 - 1801 0.174 -

Explain the output -- Processing locus 214: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTGGTTCAAAAAAGATAAGTAGCAAAATCGTATTTAAGAAAGACAGTTTCTTGCGGAGGATTTTTACAAATAATTCAA CATTTTATCCAAAAAGTATAAATTGTTTTGCAAATTTTACAAATAAATAGTAGATTTATGGGAAACTTTTTCTTACGAAA TTTCGTATTGGGCAAAAAAAAAGAAAACAAACTTTGTGAAAACTCTTGTCAAAACTTGAAAGTTTTCTTAATTTTTAAAA TTTTAGGGTTTTCTTTCTCGACGTCAAAACTCTGTAGGGTCGAAAAACTTTTTCCCTATAAAATCACTCAATCTTTACAT TCTCAATACTATCCACCTATCTTAGAAATGGAGTTCTCAAGCTTTTTGTTTACTATTTTACTCTTTTCACTGAATATTTC TCCTCTTGTTTCAGCTCATGGCTCCAACCATGAAGACTTTCTTAAGTGTCTTTCTTACCGAATGAACGACAACACCGTCG AACCCAAAGTCATACACACTTCCAAAGACTCTTCCTTTTTTTCCATATTGGATTCTTCGATACAAAATCCAAGATTCTCG GTTTCCGAAACACCTAAACCGGTCTCAATCATCACTCCCGTTAAAGCCAGTGACGTTCAAACTGTGATCAGATGCGCACA GCTGCATGGAATTCATGTAAGGACTCGGAGCGCCGGTCACTGCTACGAAGGTCTCTCTTACATTGCGTACAACAAACCAT TCGCTGTCATCGATTTAAGGAATCTTCGGTCTATATCATTAGACGTTGATAACCGGACAGGTTGGGTCCAAACCGGAGCA ACGGCTGGGGAATTGTATTATGAGATTGGGAAAACCACCAAATCTCTTGCTTTCCCGGCCGGTATTCACCCAACCGTTGG TGTAGGCGGACAATTTAGCGGTGGTGGTTATGGCACATTGCTAAGGAAATACGGTTTAGCTGCGGATAACATAATCGATG CACTTGTGGTTGACGCGAGTGGGAGGATTCTTGACCGACAAGCAATGGGCGAGGATTACTTTTGGGCTATTCGTGGAGGG GGCGGATCAAGCTTCGGTGTTATACTATCGTGGAAGGTTAAATTAGTCGATGTTCCATCCACAATTACCGTGTTTAAAGT CCAGAAAACATCGAAGAAAGAGGCCGTTAGGATCATCAAGAAATGGCAATATGCTGCAGATAAGGTTCCTGATGATCTTT TCATTAGGACAACATTGGAGAGATCAAACAAGAACGCAGTACACGCTTTGTTCACTGGACTATATATTGGTCCGGTGAAC AATCTATTGGCGTTGATGGAAGAAAAGTTTCCGGAACTAGGTCTTGAGAAAGAAGGTTGTGAAGAGATGAGTTGGATTGA GTCTGTACTCTGGTTTGCTGATTTCCCTAAAGGAGAATCTCTTGGTGTTCTCACGAATCGTGAGCGTACATCTCTATCTT TCAAAGGCAAAGATGATTTTGTCCAAGAACCGATACCCGAGGCTGCAATTCAAGAGATATGGAGGCGATTAGAAGCCCCC GAGGCTCGGCTTGGAAAGATCATATTAACTCCATTTGGTGGGAAAATGAGTGAAATGGCAGAGTACGAAACACCATTCCC ACATCGAGGAGGGAATCTCTATGAGATTCAGTATGTGGCTTACTGGAGAGAAGAGGAAGACAAGAACAAGACGGAGACAG ATAAATATCTGAAATGGGTCGATAGCGTTTACGAATTTATGACTCCATATGTTTCGAAATCTCCAAGAGGAGCTTATGTC AATTTCAAGGATATGGATTTGGGTATGTATCTTGGAAAGAAGAAGACAAAGTACGAGGAAGGAAAGAGTTGGGGAGTGAA GTATTTCAAGAACAATTTCGAGAGATTGGTGAGAGTGAAGACTAGGGTTGATCCAACAGATTTCTTCTGCGATGAACAGA GCATTCCTCTGGTGAACAAAGTTACCTGAAGATATCATTTGAAGTTTTTTATTAGTCCCTTTTCTCTGTGAAATCATCTG TGCGTGTTGAATATTATGCGTCAAGTGTGTAACTTATGTGTGTGATTG ................................................................................ .........................................................N...................... ................................................................................ ................................................................................ ...........................i.................................................... .................N...........N...............................N.................. ................................................................................ .......................................................................N........ .....N........N................................................................. ................................................................................ ...................N............................................................ .............................N...............................................N.. ............................................i................................... .................................................N.............................. ..........................................i.......N....................N........ ................................................................................ ...............N..................................................N............. ................................................................................ ............i.............................................i..................... .............................................i........i......................... ....................N...........N............................................... .............N........................i.........N..........................N.... ............N...........i....................................................... .......................................................................N........ ................................................................................ ...............N...................N............ Pos Score Pred ------------------------ 138 0.194 - 348 0.838 Yes 418 0.367 - 430 0.288 - 462 0.367 - 632 0.398 - 646 0.240 - 655 0.284 - 820 0.350 - 910 0.351 - 958 0.185 - 1005 0.513 Yes 1090 0.090 - 1163 0.527 Yes 1171 0.300 - 1192 0.098 - 1296 0.320 - 1347 0.334 - 1453 0.743 Yes 1499 0.701 Yes 1566 0.685 Yes 1575 0.869 Yes 1621 0.197 - 1633 0.125 - 1694 0.373 - 1719 0.609 Yes 1729 0.133 - 1756 0.438 - 1773 0.397 - 1785 0.653 Yes 1912 0.195 - 2016 0.041 - 2036 0.073 -

Explain the output -- Processing locus 215: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAAAGTTGGAAATATTTGGCCGAAAAATCAAAAGTACACTTTCTCAAAAAGCCAAAAATTCTCTATTTATCCAAATCTC TAACAAAAAACTCAAAAGTGAAAAAAAAAAAAAAGAATGAACAAAATGTATCTTATCTTTCTTCTATTCTTCGCTGCATC GTACTCAATGTCATTGAGTTCTGCAGATTCCGTAACTATCTACGAAGATTTTGTCCAATGTTTCAAAAACGTGACAACTA TCTCCGACATTGATCTCTCCGACGTCGTTTTACCACGTACTAGCATTTCCTTCACTCCTACCTTACGCGCCTACATCAGA AACGCCCGTTTCAACACCTCCTCAATGCCAAAACCGTCGATCATCATCGTGCCCCGTGTCGATTCACACGTTCAAGCCGC CGTGATTTGCGCCAAAACGCTTAATCTCCAGCTGAAGATTCGTAGCGGCGGTCATGACTACGACGGTCTTTCTTACGTCT CCGCCGTAACGTTCCTCGTACTTGATCTATCCAATTTCCGAAACATCACCGTCGATTTAAACGACGGAGGAGGATCCGCT TGGGTACAAACCGGCGCGACGCTCGGCGAGCTTTATTATAGGATCTGGGAGAAGAGCGAGGTCCATGCTTTCCCTGCCGG AGTTTGTCCTACCGTCGGCGTTGGAGGACACGTTAGCGGTGGTGGATACGGACACATGATTCGAAAATTCGGACTCACTA TAGATCACGTCGTCGACGCTACAATCGTTGACGCTAACGGTCAGATTCACGATCGGAAATCGATGGAAGAAGATCTCTTC TGGGCGATTCGCGGCGGAGGCGGCGGAAGCTTCGGCGTCGTTTTAGCTTTCAAAGTGAAACTCGTGACCGTTCCAAAAAC CGTAACCGTTTTCAGAGTTGATAAGTCTGTAGACGAAAATGCCCTTGACATGGTTTACAAATGGCAATTCGTAGCTCCTA GAACCGACCCGGGTTTATTCATGAGAGTGTTGTTATCATCTCCGACGCAGAACAAAACAAGTACGGTGAACACTAAACTC AGGGCGTTGTATCTTGGAAAAGCAGACGACGTCGTTTTAAAGATGGCGGAGGAGTTTCCGGAGTTGGGTTTGAAGAAAGA AGATTGTAAGGAGATGACGTGGATCCAATCACTCTTATGGTGGATGAATCATGTAGACGTGGACAAAGTCAAACCGGAGA TATTACTCGAGAGAGAACCGGATTCGGCTAAATTTCTCAAGAGAAAATCAGATTACGTAGAGAAAGAGATGACCAAACCA GAATTAAACCGGTTGTTTCAGAAATTGGCGACGTTAGATAGAACCGGTTTGGTTTTGAATCCGTATGGAGGGAGTTTAAA CGTGACCGCAGTGAATGCAACGGCGTTTCCGCATCGGCACAAACTATATAAGATCCAGCATTCGGTGACTTGGCCCGATG CTGGACCGGAAGCGGAAAGACTTTACATTGGTAATTTAAGAACGACTTATAATATTATGACTCCGTTTGTGTCGAAAAAT CCTAGGAGTTCGTATTTAAACTATAGGGATATTGATATCGGGGTTAATGATCATGGAGCGGATGGTTATCGAAAGGGAGA AATCTATGGGAGGAAGTATTTTGGCGAGAATTTTGATCGATTGGTTCGTGTTAAAACGGCTGTTGATCCGGATAATTTCT TTAGAAATGAGCAGAGTATACCAACTTTACCTCCTAATAGAAGATAGATAACTTTAGTCCATGTAACAATAGTAACTTCC TTATAGTGTTTTTTTATGAAACTTGGAATATTTTTTAATAGATTTAGCTGCAAGCTATATTGTGGC ................................................................................ ....................................N........i.................................. .......N.................................................N...................... ................................................................................ ........................i....................................................... .....................................................i.......................... ................................................................................ ................................................................i............... .......................................................i........................ ..............................................................i................. ................................................................................ ......................................N..........i..........i................... ....................N........................................................... ..........................................i..................................... .............N......................N......N......N............................. ....................................................................i........... ................................................................N............... ..............N..............................................................N.. ........................................................i....................... ..............................................N.....N........N.................. .....N.......................................................................... ......N.....................................................N................... ...............N.................................................. Pos Score Pred ------------------------ 117 0.480 - 126 0.501 Yes 168 0.475 - 218 0.471 - 345 0.849 Yes 454 0.681 Yes 625 0.565 Yes 696 0.831 Yes 783 0.692 Yes 919 0.453 - 930 0.680 Yes 941 0.699 Yes 981 0.300 - 1083 0.631 Yes 1134 0.282 - 1157 0.460 - 1164 0.175 - 1171 0.241 - 1269 0.737 Yes 1345 0.190 - 1375 0.282 - 1438 0.157 - 1497 0.649 Yes 1567 0.177 - 1573 0.116 - 1582 0.107 - 1606 0.084 - 1687 0.184 - 1741 0.089 - 1776 0.052 -

Explain the output -- Processing locus 216: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTTTCTATTTTCTCTCACAAAAGAGAAACCAAACAAGGTTTTGATTAATTTGTTTAGCTAAAAATAAATAAACTGCATT ATACACTTCAAATCGTGCTAATTAGGAAGCCCAAATCATATGGAGGACGAGCCACTTTTACCACTTATGGGCCTGTTATT AGTGGCCCAAGACGAGACGGATGTCGGATCATGCGATTTGGCTGTTGCAATCCATAGACACCACCGAGCGAGCATCTTCT TCTTCTTCTTCGCTAACCGGAGTAATCGTAAGCAGCTTCGACTGGTGAAAATGTGTTACTCTCTCTCTATACAATCTTCA ATTGACTTCCATAATCGAAACGCTCTAAAAATCCATGGAGATCGAGCAATTTTGACTTCGAACTTACCTACACTCAGGAG GATTCCGTTTTTGCCGGAGCGAGATCGCCGGAGAAAGTTGGTCCTCTGTACCGGGAGAGTCGTAAACTCATTGAAATTCA CCGGAAACACTTCCGTCGATCTATGTGGTATTCCTCGTCATCGATTAAGAGTTTCTTGCAGCGATGCTCGCCGTACACCG GAAGAAACGGCGGCGGAGTTGACGGCGCAACCCAATTTTTCAGAATTTATAACGTCGGAGAGAGTCAAAGTGGTTGCGAT GCTCGCGCTTGCTTTGGCGCTATGCAATGCGGATCGTGTTGTGATGTCCGTTGCGATTGTTCCTCTTTCGCTTTCTCGTG GATGGAGCAAATCATTTTCCGGTATCGTTCAGTCATCGTTTCTATGGGGATACTTAATTTCTCCAATTGCTGGTGGAACT TTGGTGGACCGTTATGGTGGTAAAGTAGTCATGGCGTGGGGTGTGGCTTTGTGGTCGTTGGCAACTTTTCTTACCCCTTG GGCGGCTGATAGTTCTTTGTGGGCTCTTCTTGCTGCAAGAGCGATGGTTGGGGTTGCTGAAGGAGTGGCTCTTCCTTGTA TGAACAACATGGTTGCGAGATGGTTTCCCCCAACAGAACGTTCTAGGGCGGTTGGTATTGCAATGGCAGGGTTTCAGCTT GGGAATGTTGTAGGACTCATGTTGTCTCCTATTCTTATGTCTCAAGGAGGTATATATGGCCCATTTGTAATTTTTGGACT ATCGGGGTTCCTTTGGTTGCTCGTGTGGCTCTCTGCAACGTCAAGTGCACCAGATCGACATCCTCAGATTACAAAGTCTG AACTTGAGTATATAAAGCAAAAGAAGCAAATATCTACTATGGAGAACAAACGAATTAGCACAAGTGGAATACCTCCTTTT GGACGCCTTCTCTCCAAGATGCCGACTTGGGCTGTTATAGTTGCAAATTCCATGCATAGCTGGGGTTTCTTTGTGATTCT TTCATGGATGCCCATATACTTCAATTCAGTATATCATGTGAACCTCAAACAAGCTGCTTGGTTTAGTGCCGTTCCATGGA GTATGATGGCTTTCACAGGATACATTGCTGGTTTTTGGTCGGATTTGTTGATACGGCGTGGTACAAGCATCACCTTAACG CGAAAAATCATGCAGTCCATTGGTTTCATTGGTCCTGGAATTGCTCTCATTGGTCTAACGACGGCAAAACAACCTTTAGT GGCATCAGCCTGGCTTAGTTTAGCCGTTGGGCTTAAATCATTCAGCCATTTGGGGTTTCTTATTAATCTTCAGGAAATTG CTCCCGAATATTCCGGTGTTCTACATGGAATGTGTCTTACTGCTGGGACATTAGCTGCGATAGTTGGCACAGTTGGAGCT GGTTTCTTTGTAGAACTATTGGGCTCCTTCCAGGGATTCATCCTTCTTACAGCAATATTGTATCTCCTTTCTGCTCTCTT TTACAACATATACGCAACTGGAGAAAGAGTAGATTTTGACACAACAGCTTGAAGGTGACCACAAAGTCACTTAATAGGTG GATCAGCTAGTAAAGAAGTTGCAACTCTATATAGAATCAGCTTTTGCTCTCCAAGTAAATACCAGGAACAA ................................................................................ .......................................i..........................N............. ....................i.........N................................................. ..................................................i............................. ..................................i............................................. ................................................................................ ......................N........................................N................ ..............................................................................N. .....................N....N................N.................................... .............N................i................................................. ...........................................i...................................N ........i..........i..........................................i................. ....N.............N.................N..................N........................ ................................................................................ ......................................i......................................... ..................N................................N............................ ...N...N...........................N.......................................i.... ..N..i.......................................................................... .........i...................................................................... ................................................................................ ........................N....i.................................................. ................................................................................ ................................................................................ ....................................................................... Pos Score Pred ------------------------ 120 0.731 Yes 147 0.197 - 181 0.737 Yes 191 0.478 - 291 0.664 Yes 355 0.552 Yes 503 0.202 - 544 0.426 - 639 0.360 - 662 0.208 - 667 0.304 - 684 0.277 - 722 0.224 - 764 0.244 - 814 0.135 - 831 0.860 Yes 924 0.701 Yes 960 0.228 - 969 0.543 Yes 980 0.603 Yes 1023 0.519 Yes 1045 0.156 - 1059 0.227 - 1077 0.173 - 1096 0.414 - 1239 0.790 Yes 1299 0.192 - 1332 0.239 - 1364 0.262 - 1368 0.301 - 1396 0.279 - 1436 0.540 Yes 1443 0.220 - 1446 0.738 Yes 1530 0.609 Yes 1705 0.262 - 1710 0.688 Yes

Explain the output -- Processing locus 217: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTTTTACGTAGTAGGAAAATTCTTAATTTTATTTAATTAATTACTTAAAATAATATAATCAATTTGAATAAATTAGTT TAAGTTGAATTAATAAATTAATACTATAATCTAAAGAATTTTAGTTTGACTAACATAATATTCTTCTCCCCAGTTTATCT TCTCTTTTTATTTAATATGTTGTATATGTTCTTCGTATTTATATTGTTACTTTTGGGAGTTGATCTAAGAACCACAATCT TTCACTTTTGTTACATTTCAGTTTTGTCTTTTAAAATTTTCTACTATCACTACAATCTTAAAGAAAAAATGATAAATAAT CATCCAATTAGAGAAAAACCAAAACACATTATTTTCAACTTACTGTCACTAATCTTTTTCCTAATCTTCTTATCCACCGT TGTTTCATCTCAATCACCATCATACACCACTCACAAAACCCAGCGTTTAACCGAAACCAAAACCATCCCCGAGCTTATCA TCGCGGACTTAAACCTAACCATCTTAAAGGTTAATCTCGCTTCCTCCAACTTCTCCGACCTCCAAACCCGTCTTTTTCCA AACCTAACCCACTATGAACGTTGCGCATTCGAAGACTGTCTCGGGCTACTCGATGACACCATCTCCGACCTCGAAACCGC AGTCTCCGATCTTCGATCTTCCTCTCTTGAGTTCAACGACATCAGCATGTTGCTCACCAACGTCATGACATACCAAGATA CGTGTCTTGATGGTTTCTCGACAAGTGACAATGAGAATAATAATGATATGACGTATGAATTGCCTGAGAACTTGAAGGAG ATCATCTTAGACATCTCTAACAACCTTAGCAACTCCCTCCATATGCTTCAGGTGATTTCCAGGAAGAAACCTTCCCCGAA ATCATCAGAAGTTGACGTTGAGTACCCGAGTTGGCTCTCAGAGAATGACCAGAGACTCCTTGAGGCTCCGGTACAAGAGA CCAATTACAATCTCTCGGTGGCGATTGATGGTACCGGAAACTTCACAACCATTAATGATGCCGTCTTTGCTGCTCCCAAC ATGAGCGAAACTAGATTTATTATCTATATAAAAGGTGGGGAATATTTTGAGAACGTCGAATTACCTAAGAAGAAGACAAT GATAATGTTTATCGGAGATGGTATCGGAAAAACAGTCATAAAAGCAAACCGCAGCCGCATTGATGGATGGTCAACATTTC AAACTCCTACCGTAGGCGTGAAAGGTAAAGGCTACATAGCAAAGGACATATCATTCGTGAATTCTGCAGGACCGGCCAAA GCACAGGCTGTGGCCTTTCGAAGCGGCTCTGATCACTCTGCCTTTTACCGTTGTGAGTTCGATGGTTACCAAGACACCCT TTATGTTCATTCAGCCAAACAATTTTATCGAGAATGCGATATATATGGTACAATCGATTTCATATTCGGCAATGCGGCTG TTGTGTTCCAGAATAGTAGTCTTTATGCTCGTAAACCTAATCCTGGACATAAAATTGCATTCACCGCTCAAAGCCGCAAT CAGTCGGACCAGCCCACGGGTATATCTATACTTAATTGTAGAATCTTGGCCGCACCTGATTTGATCCCTGTGAAAGAAAA CTTCAAGGCGTACTTAGGTCGACCATGGAGAAAATATTCTAGGACGGTTATCATAAAATCATTTATTGATGATCTTATAC ATCCCGCTGGATGGTTGGAGGGGAAAAAAGATTTTGCACTTGAAACTTTATATTATGGAGAGTATATGAATGAAGGGCCT GGGGCAAACATGGCAAAGCGAGTCACATGGCCTGGTTTTAGACGCATCGAAAACCAAACGGAGGCTACCCAATTCACAGT CGGTCCATTTATCGACGGTAGCACATGGCTAAATTCAACTGGCATCCCTTTTAGTCTTGGTTTCTAATCGTTAAATTTGA TAATAATGAAGCAATCTGTTGTAACAATCATATATTGTATCGTAAACATTGGACTTAAACTTAAAATGTTTTTTGTCAAC ATTCTT ................................................................................ ................................................................................ ................N........N...................................................... ....................................................................N........... ................................................................................ ................................................................................ ................................................................................ .............i......................................N........................... ..............................................i.................i............... .........N....................N...........N....i......N......................... ..........................................N..................................... ............................................i................................... ...........................N..........................N..N...................... i.............................................................................i. ....i............i............................................i...N............. ................................................................................ .............................................................N.................. ..N..............................N..........N..........................N........ ........................N....................................................... ................................................................................ ........................i...........................................N........... ..........i...........................................N..........i...N.......... .........i................N..................................................... ........................i....................................................... .....N...........................................................N.............. ...... Pos Score Pred ------------------------ 177 0.147 - 186 0.115 - 309 0.310 - 574 0.653 Yes 613 0.372 - 687 0.593 Yes 705 0.542 Yes 730 0.264 - 751 0.109 - 763 0.163 - 768 0.800 Yes 775 0.082 - 843 0.482 - 925 0.502 Yes 988 0.272 - 1015 0.207 - 1018 0.348 - 1041 0.654 Yes 1119 0.567 Yes 1125 0.580 Yes 1138 0.658 Yes 1183 0.514 Yes 1187 0.396 - 1342 0.225 - 1363 0.075 - 1394 0.294 - 1405 0.467 - 1432 0.467 - 1465 0.116 - 1625 0.522 Yes 1669 0.237 - 1691 0.522 Yes 1735 0.407 - 1746 0.600 Yes 1750 0.237 - 1770 0.692 Yes 1787 0.407 - 1865 0.610 Yes 1926 0.072 - 1986 0.081 -

Explain the output -- Processing locus 218: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCTGAAATGTCTTTAGTCCGTTTATTTATCAGTTGGTTGTGGAAAAGACCTATATTTTGAACTTTATCAGACTGCAGTT TTAGCATGTTCTTTAAATTAATCTTTCTTTAAGAAATGGTGGATCAACGGATTTATGGTTGTTCTGTTTTCCTGCAGAAT TCCTACATCTCCTATGAGAGCATGCAGAAGCTTGGGATTTCTCAAGCTGTGCAGTTCTTACAGATACCCAGATGGCACAC CTTTAGCTGCTGTATTTATTCATTCCTCAATGATTGAGATGCTGGATTCTGCAACTGAGGATGCTATTGAACAAAACAGT TGGATCGATTCCCTCTCTGTATTACCTTCATCGTTCACGTCACAGGATGCAAACAAGATGTTGCTTCTTTGTCCCTCTGT CCAATCAGCTCTCAAGGCTGAGAAAGCACTTATCTTGGGCGAATCCTATGTCCTAAGCAGTGGCTTTATCAAGGGAATAT ATGATCAAATTGAGAAAGAGGCAGATGCTTTTAGCATCCAAGCTTCTACTGCTACTTTGATACATCCTTCAAGCAAGTCG TCAGAGTCGACAGAAAGCATACCTGCCAATACAGATAAAGGGTCAAAGAAGAAAAAAGGAAAGTCTGCAAGCACGAAGGC AGCTACCGTTGAAACTGTTCCAGATGATGAAGAGGATGCCCGTCCAAAATCTAAGAGAAACCAGAAGAAAGGAAGGGATT CATCTTCATCACAGAAGTTGGATTCAAAAGCAGGAGGCAAGAAAGAGTCAGTTAAGGCGCAAGAAAGTAATAACATTATT CCGCCAGACGAATGGGTGATGAAAAAGATCGTCGACTCTGTACCTGAATTTGAAGATGATGGTACGGAGAACCCAGATTC GATTCTTAAGCATTTAGCTGATCATATGAAACCGATGCTCATTAATTCACTGAAGGAGAGAAGAAAGAAAATCTTCACAG AAAATGCAGATAGAATGAGGCGCTTGATTGATGATCTGCAGAAAAAGCTTGATGAGTCTTTCCTAAATATGCAGCTGTAC GAAAAAGCGCTAGACCTTTTCGAAGATGACCAATCAACTGCAGTTGTTTTACATAGGCATCTGCTGAGAACAACAGCAGC TACAATTGCTGATACACTTCTTCATGGCTTGGACATCCACAACAAAATGAAGAATGGTACTGAAGTTGAAGAATCCAAAA CGCAAGATCTAGTGTTGCTTGATTCTTCTGAGAGAACAGCCCTTGCTAAGAATCTCAACGGTTCACTTTCAAAAAAGGCT CTTGCACTAGTTGAGGCATTGGAAGGAAAGCGTGTAGACACTTTCATGGTCACCTTCAGAGACCTGGCAGAAGAAAGTGG GTTAGTCTTGAAGAAGCTTGACAAAAAACTGGAAAGGACACTCCTACATTCATATCGTAAGGATTTGATATCTCAAGTTT CCACCGAATCAGACCCTATTGCACTTCTCGCTAAAGTTGTCTCCCTCCTTTTTATTAAGATTCACAATAAAGCTTTACAA GCCCCTGGTAGAGCCATTGCTGCGGCTATTTCACACTTGAAGGAAAAACTTGATGAATCTGCGTACAAGACTCTGACAGA TTACCAAACAGCAACTGTAACTCTTCTTGCTCTAATGTCAGCTTCATCCGGCGAGGAACATGATTGCTCCGCAGACAGAA TCTTGACCAAACGGGAACTTCTCGAGAGCCAGATGCCATTGCTTAGAACCCTCGTCCTTGGTGACTCGCAGCCACAACAA TCCTAATCCAACAAAGTCAGGTCGGAAAGTTTCTTTAGTGTTCTGCTGACAAGACTGAGGTCCCTTCACTTAAAAACTTA TTAGGACTAGGAGAAATGACACTTC .......N........................................................................ .....N.............................N..................N......................... .............N.......N.................................................i........ .............................i........i.....................N................... ..............................................N..........i...................... ...............................................N................................ N.......................N....................................................... ................................................................................ .......................i..N........N............................................ ................................................................................ ...........N......N....................................N..N..................... .........................N........N............................................. ...N..........i...............N....................N................i........... .........................N...................................................... .......................N......................i......N.......................... ................................................................................ .............................................N.................................. ................................................................................ ................................................................................ ....................................................N........................... ..................................N........................i.................... ................................N............................................... ................................................................................ ...............N......... Pos Score Pred ------------------------ 8 0.148 - 86 0.258 - 116 0.459 - 135 0.340 - 174 0.342 - 182 0.221 - 232 0.517 Yes 270 0.713 Yes 279 0.576 Yes 301 0.165 - 367 0.166 - 378 0.630 Yes 448 0.122 - 481 0.274 - 505 0.357 - 664 0.508 Yes 667 0.195 - 676 0.180 - 812 0.496 - 819 0.329 - 856 0.139 - 859 0.175 - 906 0.417 - 915 0.401 - 964 0.383 - 975 0.507 Yes 991 0.164 - 1012 0.192 - 1029 0.546 Yes 1066 0.351 - 1144 0.233 - 1167 0.736 Yes 1174 0.378 - 1326 0.330 - 1573 0.281 - 1635 0.400 - 1660 0.581 Yes 1713 0.378 - 1856 0.120 -

Explain the output -- Processing locus 219: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGAAAGACAAACTGTCATGAATTTTCCAACCGGATAAATCAGATTACCCGATTCAAGCCCAATTTAGAAACCACATGCC CACTCTAGTCTTATATATTAGGGGATTCATAAGAAGGCCCAACACAGGACCAATCTTATGACCCAATACAAGTTAACAAA CTCATGTAATAATTCTCATTATTGTTCCGATGATAAGAGAATGATTCAGAGAATCAATGCCTTGAGCTTAAGCTCTGGTC TAAATTGGTTCGTTACCTCATTGAAAATCCATGGAAATAACCTCAAAACATTAAAGCAATCTCATTGTTACATGATCATA ACCGGTTTAAACAGAGACAACCTCAACGTTGCCAAATTCATCGAAGCATGTTCAAATGCAGGACATCTCCGATATGCTTA CTCTGTATTCACCCACCAGCCATGTCCCAATACTTATCTCCACAACACCATGATTAGAGCTTTGTCTCTCCTCGACGAAC CCAATGCGCATTCGATTGCGATCACGGTTTATAGAAAGTTATGGGCTTTGTGTGCTAAGCCTGACACGTTTACTTTCCCG TTTGTGTTGAAGATTGCTGTTAGGGTTTCTGATGTTTGGTTTGGGAGACAGATTCATGGACAAGTTGTGGTTTTTGGGTT TGATTCGAGCGTTCATGTTGTAACAGGTTTGATTCAGATGTATTTTTCGTGTGGTGGTTTGGGAGATGCACGTAAGATGT TCGATGAAATGCTTGTCAAGGATGTGAATGTTTGGAATGCGTTGTTAGCAGGGTATGGGAAAGTTGGTGAGATGGATGAA GCTAGGAGTTTGCTTGAGATGATGCCTTGTTGGGTAAGGAATGAGGTTTCTTGGACTTGTGTGATCTCTGGATATGCGAA AAGTGGACGAGCTAGCGAAGCTATTGAAGTGTTTCAGAGAATGCTGATGGAGAATGTTGAGCCTGATGAAGTTACGCTTT TGGCTGTGCTCTCAGCTTGTGCTGATCTTGGTTCACTTGAATTGGGTGAAAGAATATGTAGCTATGTTGATCACCGAGGG ATGAACAGAGCGGTTTCATTGAACAATGCGGTCATTGATATGTATGCGAAATCAGGGAATATAACAAAGGCGTTGGATGT GTTTGAGTGTGTTAATGAGAGAAATGTTGTTACTTGGACGACTATAATCGCAGGGCTAGCTACGCACGGGCACGGAGCTG AAGCCTTAGCGATGTTTAACCGTATGGTTAAAGCTGGCGTGAGACCGAACGATGTTACTTTCATTGCAATCCTTTCAGCT TGCAGTCATGTCGGTTGGGTTGATCTTGGAAAACGTTTGTTTAACTCAATGAGATCGAAATACGGGATTCATCCAAACAT TGAGCATTACGGATGCATGATTGATTTGCTAGGACGAGCAGGTAAACTCCGAGAGGCGGATGAAGTTATTAAAAGCATGC CCTTTAAGGCCAATGCTGCAATATGGGGATCTCTTCTTGCTGCTTCAAATGTTCATCATGATCTTGAGCTTGGAGAAAGA GCTTTGAGTGAGCTGATTAAGTTAGAACCGAATAACAGCGGTAACTATATGCTTTTAGCTAATTTATACTCGAATTTAGG AAGGTGGGATGAGTCGAGGATGATGAGGAACATGATGAAAGGGATTGGTGTGAAGAAGATGGCTGGTGAAAGCTCCATAG AAGTTGAAAACAGAGTATACAAATTCATTTCAGGGGATCTTACGCATCCTCAAGTCGAAAGGATTCATGAAATTCTACAA GAGATGGATTTGCAAATACAGAGCAAAGTTTGAGAAAAAAAAAATTATTTGAATTTTGATCAGATTGAGTTGATCAAACA ATGTCTTCTCTTCTAAGACCTGAAACCGGAGGAGCCGAGTCAATGAGGCTGA .................N.........................................................N.... .........................................................N...................... ...N.........................N..........i...............N....................... ..............................N........................................N........ ...............................................N.......i.................N...... .....................N...........................N.............................. ...N....................................i....................................... ...............................N.......................N........................ ..............N......................N...........................N..........i... ...N....i............N.....N........N.................N................i...N.... ..................N..N..................N................................N...... ........................................N.....i......N...........N.............. .......................................................N.......N................ i........................N.............i...N................................N... ..............N........N........................................................ ...........i...........N...........................N............................ .......N........................................i............................... ............i...i..........................................N................N... ............N.........i.........................N........N...................... ................................................N............................... ........N..........N..N........i..N.......................i..................... ..................................................................N............. ...N............................................................................ N.........................................N......... Pos Score Pred ------------------------ 18 0.434 - 76 0.157 - 138 0.438 - 164 0.264 - 190 0.115 - 201 0.516 Yes 217 0.251 - 271 0.265 - 312 0.399 - 368 0.280 - 376 0.632 Yes 394 0.297 - 422 0.375 - 450 0.362 - 484 0.117 - 521 0.647 Yes 592 0.093 - 616 0.377 - 655 0.069 - 678 0.479 - 706 0.281 - 717 0.747 Yes 724 0.076 - 729 0.609 Yes 742 0.085 - 748 0.259 - 757 0.299 - 775 0.200 - 792 0.771 Yes 796 0.215 - 819 0.245 - 822 0.323 - 841 0.243 - 874 0.104 - 921 0.397 - 927 0.677 Yes 934 0.287 - 946 0.430 - 1016 0.432 - 1024 0.198 - 1041 0.524 Yes 1066 0.231 - 1080 0.746 Yes 1084 0.095 - 1117 0.224 - 1135 0.127 - 1144 0.262 - 1212 0.749 Yes 1224 0.339 - 1252 0.177 - 1288 0.343 - 1329 0.620 Yes 1373 0.589 Yes 1377 0.572 Yes 1420 0.187 - 1437 0.483 - 1453 0.076 - 1463 0.502 Yes 1489 0.110 - 1498 0.162 - 1569 0.395 - 1609 0.293 - 1620 0.440 - 1623 0.410 - 1632 0.504 Yes 1635 0.169 - 1659 0.634 Yes 1747 0.096 - 1764 0.367 - 1841 0.147 - 1883 0.041 -

Explain the output -- Processing locus 220: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATACAAGAGAGTATCACGTGGTTGTGAAGCTTTTTGTATTAAATTTGTCTTAGTCGAATAGAGAAATATATTTGTTTTCT TTGCAATTTGATCATATTTGAGTTTCTTTTTTGTCAACTTTGTATATTTTAGTTTGTGTTCACATAATTCTGTGTATTTA TATCATTAAGTATATATGCATGACATCCTCCTCCTGCATTTGCTCCCTATATTCCTCATTATTATTAGTCATGGGAAGAG AGAAGACGTTATGCAAGTCGTGGAGCGACATGAAGAAACACTTAAACGACACCGTTTCCAAGAGCTTCATCGGCCGTTTC TTCAAGCTCGAAGCAAGAAAAACAACGTTCACAACTGAACTACGAGCTGCGACCGCCACATTCCTCACCATGGCTTATAT CATTACCGTGAACGCCAATATCTTAGCCGACTCCGGTGCCACTTGCTCCATCAACGACTGTTCCACCGTGGCCAGTTCTT CACCACCCGGTCCAGAATGCGTCCTCGGGTCTAACCCTGGCTACGAACAATGTATTTCTAGGGTTAAAAAGGACCTCGTT GTGGCTACTTCTTTATCAGCCATGGTGGGTTCATTAGCAATGGGTTTGTTAGCTAATCTCCCTTTTGGACTTGCTCCGGG TATGGGAGCCAACGCTTACATCGCATACAACGTTGTCGGGTTTCGTGGATCCGGTTCTATCTCTTATCATACCGCTATGG CCATTGTGCTCCTTGAAGGATGTGCCTTTCTCGCTGTGTCGGCTTTAGGACTCCGCGGGAAACTTGCTCGTCTCATCCCT CAAACGGTGAGACTGGCCTGCGCGGTCGGTATAGGAATGTTCATTGCCTTTGTAGGTTTACAAATGAACCAGGGCATTGG CCTCGTGGGACCGGATAAGTCAACTCTAGTGACATTAACCGCCTGCGCAGAGACGGATCCTGTCACTGGAGCTTGCTTAG GAGGTAAAATGAAGAGTCCTACGTTTTGGTTAGCCGTTGTCGGGTTTCTTATAACGAGTTTCGGTCTAATGAAGAACGTC AAAGGTAGTATGATTTACGGAATCGTGTTCGTCACGGCTATTTCTTGGATTAGAGGCACTCAAGTCACAATCTTTCCACA CACTCCTCTTGGAGACTCAAACTACAACTACTTCACAAAAATCGTTGACTTTCACAAGATCCAGTCAACTTTAGGAGCCA TAAGCTTCACGGAGTTTAGAAAGAGCGAGGTTTGGGTCGCGTTCGCTACACTCTTCTACGTTGACCTTCTTGGTACGACC GGAGTACTCTACACAATGGCTGAGATCGGAGGATTCGTGGAAGATGGGAAGTTTGAAGGAGAGTACGCGGCGTACTTAGT AGACGCCGGCTCCTCCGTGGTGGGATCGGCGTTAGGTGTTACAACGACGGCGACTTTTGTGGAGTCTTCGGCGGGGTTGA AAGAAGGAGGGAAAACGGGGCTAACGGCCGTTATCGTAGGGTTGTATTTCTTGGCGTCAATGTTTTTTACGCCGTTAGTT ACTAACGTGCCGAGGTGGGCGGTTGGACCGTCGTTGGTGATGGTTGGTGTGATGATGATGGGAGTTGTAAAAGATATTAG ATGGGGAGAGACTAAAGAAGCCGTGACAGCATTTGTAACGATCTTGCTTATGCCGTTGACTTATTCCATTGCTAATGGGA TTATTGCTGGTATTGGTATATATCTTGCATTAAGCATGTACGATGTCGTTTTGGGAGTTGCAAAATGGCTTAACGGGGTT AGAAAACGGGTTATGAGAGAGCATAATCAAGTTTCTTCCGTCGCTACCGTCGAGATCGTTTGATTATTAACGATAACCGA TGTCACAAAACTGGTTTTAGTGGTGTGTGAGTGTGTACCGGAACTGGTGGAATTTTTCACATGGTCGGTCGTTGGATGAC CA ................................................................................ ................................................................................ ...............i...N..................................................i......... ..........N..................i.................................................. .....................................................................i.......... ................................................................................ ................N................................i.............................. .....................i.................i........................................ ...................i............................................................ ....................................N..........................i................ ................................................................................ ........N...........................................................N........... .........N...................................................................... ................................................................................ ................................................................................ ...............i...........................N.................................... ................................................................................ ...........................................................N.................... .......................................N...........N..N..N...................... N................................................N........................N..... ...................................i......N.....................i............... ............N..................................................................N ............................................................N..............N.... .. Pos Score Pred ------------------------ 176 0.694 Yes 180 0.340 - 231 0.655 Yes 251 0.395 - 270 0.681 Yes 390 0.820 Yes 497 0.339 - 530 0.713 Yes 582 0.524 Yes 600 0.804 Yes 642 0.492 - 717 0.771 Yes 740 0.646 Yes 837 0.437 - 864 0.573 Yes 969 0.466 - 1029 0.256 - 1050 0.253 - 1296 0.833 Yes 1324 0.267 - 1500 0.188 - 1560 0.347 - 1572 0.166 - 1575 0.243 - 1578 0.215 - 1601 0.415 - 1650 0.255 - 1675 0.203 - 1716 0.764 Yes 1723 0.213 - 1745 0.805 Yes 1773 0.444 - 1840 0.123 - 1901 0.084 - 1916 0.043 -

Explain the output -- Processing locus 221: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTAATATTTGTATTAAAATTTTATACTTCATAATAATATTTTTTTTTTTCCACTCTCTCTATTTTTTAAAAAAATAT ATATTTATGTTGATATATTAGTATGAGAGAAAAGAGTTGATGTGGGAGTCAAATATATGTTGTGATCCGTTAACTTCAAA GCAAATTACTGTTTATTCACAGAAGCTTGGACGCGTACGGCTTCGATTTCCTTATATATATATATTACACATAAATCTGC CGTCTTGGAGATTGGAAACATATCTCAAGCTTCTTCTTCGTGTTACGCACTTTCTTCTTCTTCTTCTCTCTCTCTCTTTC TTCTTCGTCTTCGATCACAGAAGACAGAATTCAATGTCGTCGACCTACAAGCTCAATCACACTGATTCATTTGCAAATGG AGATGCAAAGAGTTTGTTGCCGAACCCAGAGAATGGTTTCACTCATTTGACTTCACTTGCTCAATCAGAGAAGGCTGTTC AGGAGCTTCTTCTTCAACAGACTCCTATGCAGGCTACTGACGACCATCTCGTTGAGTTCTCTGAGGCTTTGAGAACTGTT GCAAAGGCACTAAGGGGAGCTGCTGAAGGAAAGGCGTTGGCTCAAGCTGAGGCTGCTGAATGGAAGCGTAGATACGAGTT AGAGAGGTCTAAGAACGTGGAGTTGCAGCATAAAGAACTGTCAAATGGAGTGTGTGCCGATGAATCTAATGGTCAAAGGA TGGAGCACTTAGCAAAGTCTCCGCGGCTTTATGCTCAGGAAATCAGTTCTAATGGCATGGAGACTATTTGCTCTCATGAA GTGCTCCAAGATGGTGGATTTAACAGTTTTAATAACAAATTGAAGAGGAAGGCATCATTTAAACTTTCATGGGGATGTAA GGGGATGGCTAATGATCAACACAAAAAAGAAATTGTTTCGTTTGAGAGAGGGAATATCAGCACAGCAGAGCGCAGTAGTA AACAGATTTCACTGACATGGGAGTCTGATCCACAAACTGTACTTATTATCACTAAGCCTAATTCAACTTCCGTGCGAGTT CTCTCTGTGGATATGGTCAGATGGTTGAGAACGCAAAAAGGACTAAATATTTATGTGGAACCTCGAGTAAAGGAAGAACT CTTATCAGAATCAAGTTCCTTCAACTTCGTACAAACTTGGGAAGATGTTATGATTTATGATGCAGACAAGGAAATTTCAC TTCTACACACAAAGGTTGACCTTCTTATAACTCTTGGTGGGGATGGCACAGTGCTATGGGCTGCATCGATGTTTAAAGGA CCAGTGCCTCCAATTGTTCCATTCTCCATGGGATCTCTTGGATTTATGACTCCTTTTCACAGCGAACAATACCGAGATTG TCTTGAAGCGATTTTGAAGGGTCCTATCAGTATAACACTACGACACAGGTTGCAGTGTCACATCATCAGAGATAAAGCAA CACATGAATATGAACCAGAGGAGACTATGCTTGTTCTGAATGAAGTCACCATTGACCGTGGAATATCTTCTTACCTCACA AACCTTGAATGCTACTGTGACAACTCATTCGTCACATGTGTGCAAGGCGATGGACTTATACTGTCTACAACATCTGGTAG CACCGCGTATTCACTTGCTGCTGGAGGGTCAATGGTCCATCCTCAGGTTCCTGGGATCTTATTCACGCCTATCTGTCCGC ATTCTCTATCATTCCGCCCACTAATATTACCGGAACACGTGACAGTGAGAGTCCAAGTGCCATTCAACAGCAGAAGCTCC GCTTGGGTCTCCTTTGATGGGAAAGACCGGAAACAGCTTGAAGCAGGGGATGCACTAGTGTGTAGCATGGCACCATGGCC CGTCTCAACCGCTTGTCAAGTTGAATCCACTAACGACTTCTTACGCAGCATCCACGATGGTCTTCACTGGAACCTAAGAA AGACTCAATCTGCTGATGGACCTTAGAAACAAATTCCTCCAGCTTTTTTCTCTTTCATTCTTAACCAATAACCATATATA GTCTCGACGTCTATCTATCTATCTATATGCATATAGATAAATGTG ................................................................................ ......N...............N................N................N....................... ................................................................................ ................................................................................ .................................i..........................................N... ..N.............................N............................................... ..........................i..................................................... ...........................................................N.................... ............................................N..............N........N..........i ..............................N....................N....N..................N.... ..........i.........................................................N.....N..... ....i......N.................................................................... ................i............................................................... ............N.......N...............................N........................... ............................................N....i......N..N.................... ..........................................N............i............N........... ...........................i.................N.................................. ................................................................................ ...i.....N................i............N........................................ ........N..........................i.............N.............................. ...............................N................................................ ................................................................................ ................i................................N................i.......N..... ........................................................i....................... ...............N................................................................ ..........................N.............N.... Pos Score Pred ------------------------ 87 0.154 - 103 0.248 - 120 0.323 - 137 0.303 - 354 0.661 Yes 397 0.237 - 403 0.167 - 433 0.315 - 507 0.752 Yes 620 0.393 - 685 0.375 - 700 0.209 - 709 0.303 - 720 0.603 Yes 751 0.178 - 772 0.443 - 777 0.344 - 796 0.208 - 811 0.548 Yes 869 0.268 - 875 0.392 - 885 0.519 Yes 892 0.234 - 977 0.588 Yes 1053 0.263 - 1061 0.473 - 1093 0.274 - 1165 0.202 - 1170 0.725 Yes 1177 0.148 - 1180 0.217 - 1243 0.421 - 1256 0.576 Yes 1269 0.415 - 1308 0.587 Yes 1326 0.170 - 1444 0.641 Yes 1450 0.359 - 1467 0.558 Yes 1480 0.415 - 1529 0.478 - 1556 0.512 Yes 1570 0.279 - 1632 0.479 - 1777 0.601 Yes 1810 0.310 - 1827 0.633 Yes 1835 0.319 - 1897 0.556 Yes 1936 0.391 - 2027 0.051 - 2041 0.072 -

Explain the output -- Processing locus 222: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGGCTGAAAATCATCTAAGCCTGTGGACTTGGAAGCAAAGAGATAATGCTGAGGCGCGTGAGGCACAGTCGCCAACAGAA AATCGGGATGTTTTTTTTTAACTGCAAAGCCAAAGAGACAATTGTATGGTCTCTGGTAAAGGTAAGGCAATCAAAAAAAA TATGGCTGCGTCTCCCAAGATAGGCATCGGTATTGCCTCCGTCTCATCGCCTCACCGTGTCTCCGCCGCCTCATCCGCTC TTTCTCCTCCTCCTCATCTCTTCTTTCTCACTACTACTACTACCACACGTCACGGCGGCTCCTATCTCCTCCGTCAACCA ACCCGAACTCGCTCTTCTGATTCCCTCCGCCTCAGAGTCTCCGCCACCGCCAATTCTCCGTCTTCTTCTTCTTCAGGAGG CGAGATTATCGAGAATGTAGTGATTATAGGCTCCGGTCCCGCCGGTTATACGGCGGCGATATATGCAGCGCGCGCCAATT TGAAGCCGGTGGTTTTTGAAGGGTATCAGATGGGCGGAGTTCCCGGTGGACAGTTGATGACAACTACTGAAGTTGAGAAT TTTCCGGGATTCCCAGACGGTATCACGGGTCCCGATTTAATGGAGAAAATGAGAAAGCAAGCAGAGAGGTGGGGAGCAGA GTTGTATCCAGAAGATGTTGAATCTCTTAGTGTCACAACTGCTCCTTTTACTGTGCAAACTAGTGAACGTAAGGTCAAGT GCCATAGTATTATATATGCCACTGGCGCTACAGCAAGGAGGTTAAGGTTACCTCGAGAGGAAGAATTCTGGAGTAGGGGG ATAAGTGCTTGTGCTATCTGTGATGGAGCTTCGCCTTTATTTAAGGGGCAAGTACTTGCCGTGGTTGGAGGAGGAGATAC GGCTACAGAGGAAGCCTTGTATCTCACGAAATATGCCCGTCATGTTCATTTGCTTGTTCGCAGAGATCAGTTGAGAGCTT CCAAGGCTATGCAAGATAGAGTGATCAACAATCCAAACATCACAGTGCATTACAACACGGAAACCGTGGACGTATTGAGC AACACCAAGGGACAGATGTCTGGCATTCTACTCAGAAGACTTGATACGGGTGAAGAAACTGAGCTGGAGGCAAAAGGATT GTTTTATGGAATAGGGCATTCGCCAAACAGTCAGTTATTGGAAGGCCAAGTCGAACTCGACAGCTCCGGGTACGTCTTGG TTCGGGAAGGAACATCAAATACATCAGTTGAAGGTGTATTTGCTGCAGGAGATGTGCAGGACCACGAATGGAGACAAGCT GTAACTGCTGCTGGATCAGGATGCATAGCTGCTTTGTCAGCCGAGAGATACCTCACAAGTAACAATCTTCTTGTTGAATT TCACCAGCCTCAAACTGAAGAGGCCAAGAAAGAATTCACACAACGGGATGTCCAAGAAAAGTTTGACATCACTCTTACAA AGCACAAGGGACAGTATGCTCTTAGAAAACTATACCATGAGAGTCCAAGAGTTATATTGGTACTATACACTTCACCAACA TGTGGCCCCTGTAGGACTCTGAAGCCTATTTTGAACAAGGTGGTCGATGAGTATAACCATGATGTGCATTTTGTTGAGAT TGACATCGAGGAAGATCAAGAAATTGCTGAAGCAGCTGGAATTATGGGAACCCCATGTGTGCAGTTCTTCAAGAACAAGG AAATGCTCAGGACTATATCGGGTGTGAAGATGAAGAAAGAGTACCGAGAATTCATTGAGGCCAATAAATGAGGACACCAG ATAGATATTGTTTCTGGCAGATGCCAAATCTTATCATGATTCAATTTTGTTCATTCAAATACTTTGGAGTTACTGGAGCA ACGCCATTGA .............................................N.................................. .......N.....................................i.................................. ................................................................................ ................................................................................ ..............i...............................................N................. .............................i..........................N....................... .......................................i........i............................... ..............N................................................................. ...............N................................................................ ......................N......................................................... ................................N........N...................................... ........i....................................................................... ...............N................................................................ .....N.......................................................................... ...................................................N...............i............ ....................N........................................................... ...............................................N................................ ...............N....................N..........................................N ..............................................N...........N..N.................. ...........................................N..........N......................... ..i..........................N.....................................N............ ....................N..............N............................................ .......... Pos Score Pred ------------------------ 46 0.432 - 88 0.151 - 126 0.664 Yes 162 0.771 Yes 415 0.634 Yes 463 0.178 - 510 0.505 Yes 537 0.469 - 600 0.674 Yes 609 0.577 Yes 655 0.343 - 736 0.290 - 823 0.103 - 913 0.302 - 922 0.091 - 969 0.576 Yes 1056 0.378 - 1126 0.364 - 1252 0.258 - 1268 0.627 Yes 1301 0.254 - 1408 0.252 - 1456 0.235 - 1477 0.270 - 1520 0.268 - 1567 0.254 - 1579 0.419 - 1582 0.192 - 1644 0.470 - 1655 0.418 - 1683 0.644 Yes 1710 0.112 - 1748 0.241 - 1781 0.086 - 1796 0.051 -

Explain the output -- Processing locus 223: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATGAAATGAAAAGTAAAATAATTCCGCAAACAAAGATGCTACGTGTCGTCTCTGTAAGCCAAACCTTCTTCTCCAAGG AAAAAACACCAATTAAATTAAATGAAAAATAAAATATTTCCGCAGAATAAGACTCTTCTTCCATTTGGTACAGAAAAATC GAAACTTTTTAGGGTTTTTTTTTTTGTGATAACGAGAGAGAAAAAAGTGATGGACTTGTCGGTTGAGAACGGAGGTTTAG CTCCAGGTTTTAGGTTTCATCCGACGGACGAAGAACTTGTCGTCTATTATCTCAAAAGAAAGATCCGTCGGAAAAAACTC AGAGTCGAAGCAATCGGCGAGACTGATGTCTATAAGTTTGATCCTGAGGAATTACCTGAGAAAGCGTTGTATAAGACTAG AGATCGTCAATGGTTCTTTTTCAGCTTAAGGGATAGGAAACATGGAAGTAGGTCAAGTAGAGCTACTGAACGTGGCTATT GGAAAGCAACAGGGAAGGATAGAGTCATTCATTGTGATTCGAGACCCGTTGGAGAGAAGAAGACTCTTGTTTTCCATAGA GGCAGGGCACCTAATGGCGAACGGACTAATTGGGTGATGCATGAGTATACATTGCACAAAGAGGAGCTCAAGAGGTGTGG TGGTGAAGATGTTAAGGATGCTTATGTTCTTTACAAGATTTATAAGAAAAGTGGGTCTGGTCCTAAGAATGGTGAGCAAT ATGGAGCTCCTTTTATTGAAGAAGAATGGGCTGAAGATGATGATGATGATGTTGATGAGCCTGCTAATCAGCTCGTTGTT TCGGCTAGTGTTGATAATAGTTTATGGGGGAAAGGGCTTAACCAATCTGAATTGGATGATAATGATATTGAAGAGCTGAT GAGTCAGGTTAGAGATCAGTCTGGTCCAACATTGCAGCAGAATGGGGTGTCTGGACTGAACTCTCATGTAGACACGTATA ATCTGGAGAACCTGGAGGAAGATATGTATTTGGAAATCAATGATCTTATGGAACCTGAACCTGAACCAACTTCTGTGGAA GTCATGGAGAATAACTGGAACGAGGATGGTTCTGGTCTCCTGAATGATGATGATTTCGTTGGTGCTGATTCATATTTCCT TGATTTGGGAGTGACAAATCCTCAGTTAGATTTTGTTAGTGGTGATTTGAAAAATGGGTTTGCACAAAGTCTTCAGGTGA ATACTTCTTTAATGACTTACCAGGCCAATAATAACCAGTTCCAGCAGCAATCAGGGAAGAACCAAGCTAGTAACTGGCCA CTCCGTAACAGCTATACCAGACAGATAAACAATGGATCATCGTGGGTGCAGGAGCTAAACAATGACGGACTTACCGTTAC CCGGTTTGGTGAGGCGCCTGGTACAGGTGATTCATCTGAATTCCTAAACCCTGTTCCTTCTGGTATAAGTACAACTAATG AAGATGACCCGTCAAAAGACGAGTCTAGTAAGTTTGCTTCTAGTGTATGGACTTTCCTGGAATCCATTCCTGCAAAGCCA GCATATGCATCAGAGAATCCATTTGTGAAGCTGAACCTTGTTAGAATGTCAACCAGTGGTGGTCGTTTCAGGTTTACTTC TAAAAGCACAGGTAATAATGTTGTTGTTATGGATAGTGACTCAGCAGTGAAGAGGAACAAGTCTGGAGGAAACAACGATA AGAAGAAGAAGAAGAACAAAGGTTTCTTTTGCTTATCGATCATTGGGGCTTTATGTGCTTTGTTTTGGGTGATCATAGGA ACAATGGGAGGTTCAGGGAGGCCTTTGTTATGGTGAGAACCGAAAAATCCAAGAGGTTAAGAGACATAAAGGCTTGGTTT TGTGTGAACCATTAGAGAGTCAAGTCATTGTAATTATTCTCTTGGATTATTAGAT ..N....N.............................N.......................................... .....................N.......................................................... .................................................i.............................. ................................................................................ .........................i...................................................... .........N...............................i...................................... ................................................................................ .............N......................i...N....................................... ........N........N.....N............................................N........... N........................N..........N..N..N..N..N.....N......................... .......................N...............................N.....N................i. .........................................N.......................N.............. .......................i...............N.......i................................ ...i.....................N.................N..N..N.............................. .....................................................i.......................... ...........i.................................................................... ...............................i.............................N.................. .............................................................................N.. ...N..........................................N................................. ....N........................................i.................................. .................N..........i................................................... ....................................................N........................... ...i.........................N.................................................. ....................................................... Pos Score Pred ------------------------ 3 0.241 - 8 0.428 - 38 0.349 - 102 0.496 - 210 0.730 Yes 346 0.512 Yes 410 0.261 - 442 0.589 Yes 574 0.356 - 597 0.518 Yes 601 0.114 - 649 0.175 - 658 0.217 - 664 0.189 - 709 0.119 - 721 0.074 - 746 0.460 - 757 0.100 - 760 0.053 - 763 0.084 - 766 0.151 - 769 0.068 - 775 0.073 - 824 0.386 - 856 0.068 - 862 0.208 - 879 0.770 Yes 922 0.336 - 946 0.087 - 984 0.780 Yes 1000 0.075 - 1008 0.771 Yes 1044 0.740 Yes 1066 0.215 - 1084 0.078 - 1087 0.160 - 1090 0.113 - 1174 0.569 Yes 1212 0.586 Yes 1312 0.778 Yes 1342 0.405 - 1438 0.164 - 1444 0.330 - 1487 0.365 - 1525 0.137 - 1566 0.780 Yes 1618 0.213 - 1629 0.680 Yes 1733 0.412 - 1764 0.531 Yes 1790 0.198 -

Explain the output -- Processing locus 224: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAATTTTATAGATTTTACACATCTGAATCTTTCTCTTGTTTTAGTAACCATGTCTAATAACTTATTCATGGATCAGATAA CATTATGTTATGACATTTTGAATGAGCCAAAGAACATTTGAATCTTTATGTAAGATGCTAACTAGGCGATATGGATTACA AGAGCTTGAAAATGTCTATCTTGAGGAATCTGTTGCCATGTTTCTCGAGGAGGTTGATAAGAATAGGACTGTTCGAGATA TAGTTGCAAGATATCAACAGTCATTGAATGTGGTGAAAAGAAAGATTGATGATGTTTTGAGCGCTCTTTTAAAGTTTGCA GCGGATACATTAAAGTCACAAGAATCACATGATACAAAGGTTTTGAAGTATTGTGCGAGGAATGAATCTTTTTCCCCTCA TCCATCTAATCGAAAGTACTATCTTGTTAACTCTGTATATCCCACGACAACTGGATATCTTGGTCCACATCGTAGGATAT TATATCATCTTGGTCAATTTGGTAGAGGAGGACCACCAGTAACTGTACAAGAGTTGTTCAACCGAAAACATTTAGATTTG CGATCTGTGATTGATAGGACATTTGGAGTATGGAAAGCAAAATGGAGGATTTTGGATCACACCTGCAAAAATGTTAACAT CGCCATCAACGTCACGAAAACGAACATAAATCATGCATCAGCGACAACGGTAACGGCTGCGACAACAACACCAAAACAAA CAAAAATCTGCAGTAGAGTTTGTAGCCGCTGCTACCACTACAGCATGCGTCAAAAGCTACGACACTCACTACACACTCGA ATTTTAAAATCAAACAGCGAAAACGCGATTCTCTTCTCCGAGGAAGAAGAAGAAATGGCGGAGAAACAGAGATGGATTGC GATGTATACGAAACACCTCAAGCAGAAGCGAAAGGTCTATCACGATGGCTTCCTTGATTTACATATCGCCAGAAAAAAGG TGATGCTTTATGATGAGGACGATAATCTACTTGAAAGCAGGACGCTTAAGGCATGTGAAGTTGTGAACACAGGTGAAACA CTCACATTTCAAGCATATCTTGTGGATATATGCGATCCTAAGGACGGAAGTAAAGCTTCTTCTGAGCCAAAGGTTGAACC AAGTGATCAAAAATGTGCACGCAAACCATTTACAGTTCTCAGGCCAAATTTCAAGAAAAGTTCTCTTCGTTGTGATGAGA AAAAACCTGACCTTGTGAATAAATTTTCTTCGAAAAGTCTCAGCCCATCTCACAATATGATTAGAGTATTCAAAAAGAGA GAACTGCATAAGTATGGGGCACTGACTCCAGACTCAACAACAAAAGGTACTGTATCTCTTGGCTCAGTTGATAAAGAAAG CAATAACCACCCTTTGATCAACTCTCCATGTACTGGAAGGTCTGTTGTAGATTCAAGGTTAGAGAATTGTAAGTTAAGCA AGGATGTTCCTCCACCCAAGCCTTTACGTGATGTAAATCAGATACTCTCCATTCTTCAGAAACGGAATGCTACAGAGACA TGTTCTGATAATAACATGCAAACATCTGTGCTTTTGACAAAAGTACCTCCAGAATCTGAAACCAGCAAAAGACACAAAAT GGAGTCTATTTCAACGAAAGAAGCATCTACGGAACAAGTAATGGTGCAAGAAACCTCATCGGCCAGCACAAGAGGTTGCT TAATGAGTGATCCTCCGAGTTTCGACCTCGGAATTTAAACAGCCCCCTCAAGATTACAGAAAATAGGTAACTCGTTTTTT CATTTTACCTCAAGTTCTTCACGAACTATAGTTTTTTCGAAAATATAGTTTCTCATT .................................................i.................N............ ....N....N...........N.........................N......i...............N......... ...........i.........................N.......................................... ...........................N....................N..N............................ ............................N................................N.................. ................................................................................ ................................................................................ .............................N...........i............................N......... ................................i............................................... ............................................i................................... ......................................................i................i........ ..N......N..N.......................................N........................... .............................N.................................................. ............i.............................................................N..... ........................................................N....................... .............N.................................................................. ...........................N.................................................... ...N..........................N...................................N............N ...............i..............................................................i. ........................................i....................................... ..N............................................................................. ......................................................... Pos Score Pred ------------------------ 50 0.655 Yes 68 0.338 - 85 0.240 - 90 0.165 - 102 0.294 - 128 0.241 - 135 0.612 Yes 151 0.308 - 172 0.518 Yes 198 0.440 - 268 0.143 - 289 0.165 - 292 0.216 - 349 0.172 - 382 0.223 - 590 0.454 - 602 0.859 Yes 631 0.426 - 673 0.770 Yes 765 0.586 Yes 855 0.854 Yes 872 0.610 Yes 882 0.508 Yes 925 0.252 - 963 0.465 - 970 0.114 - 973 0.199 - 1013 0.378 - 1070 0.258 - 1133 0.656 Yes 1195 0.204 - 1257 0.284 - 1294 0.367 - 1388 0.309 - 1444 0.241 - 1471 0.166 - 1507 0.153 - 1520 0.356 - 1536 0.606 Yes 1599 0.835 Yes 1641 0.615 Yes 1683 0.224 -

Explain the output -- Processing locus 225: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTACGAATCTGATCTACATGCATAAACCATTTCGATTTCAATGTATTCGTGAGAATGTGCTTGAATCTGTTACAATAGT AGTGATTTTTACTAATCGTGAGAGAGTTTTTGTATTTTGATACAGGAAGGGACAATGTTTGGTGTGTATCGAGTATTCGT GTTACTGGTGTTTGTTAGTCAGCTTTGCAATGGGTTTTACTTACCAGGGAGTTATATGCACACATATTCAGATGGAGACT CTATCTTCGCCAAAGTCAATTCCTTGACTTCTATTGAAACTGAGCTTCCTTTCAGTTACTATAGTCTTCCTTACTGCCAA CCACTTGAAGGTATCAAGAAAAGTGCTGAGAATCTTGGTGAGCTTCTCATGGGTGATCAGATTGATAACTCTGCTTATAG GTTTCGGATGAGAACCAATGAGTCTCTTTACTTATGCACTACGAGTCCTTTGAATGAGCATGAGGTCAAGCTCTTGAAAC AGAGAACTCGTGAGTTGTATCAAGTGAATATGATTCTTGATAACTTGCCTGCTTTGAGATTCGCTAAGCAGAATGGGGTT ACTATCCAGTGGACTGGATATCCTGTTGGGTATTCGCCACCTAACAGTAATGATGATTACATCATTAATCACCTCAAGTT TAAGGTTTTGGTTCATGAGTATGAAGGCAATGTCATGGAGGTGATTGGTACTGGTGAAGAAGGTATGGGTGTGATCTCTG AAGCTGATAAGAAGAAGGCATTGGGGTATGAGATTGTTGGATTTGAGGTTGTTCCCTGCAGTGTTAAGTATGATGCTGAA AAAATGACGAAGCTTCACATGTATGACCCTGTTCCTTCAGTTAACTGTCCATTGGAGCTTGACAAAGCTCAGATCATTAA AGAACATGAAAGAATAACCTTCACTTATGAAGTGGAATTTGTTAAGAGTGAGACAAGATGGCCATCCAGATGGGATGCCT ATTTGAAAATGGAAGGTGCTCGTGTTCATTGGTTCTCTATTTTGAACTCGCTTATGGTGATCTTCTTTCTGGCCGGTATA GTTTTCGTCATATTCCTGAGGACAGTGAGAAGGGATTTGACAAAGTATGAGGAACTGGACAAGGAAGCACAGGCACAGAT GAATGAGGAGCTCTCTGGATGGAAACTTGTTGTCGGAGATGTGTTCAGAGAACCAGAAATGTCAAAGCTTCTATGCATTA TGGTGGGAGATGGGGTTCGAATTACAGGAATGGCTGTTGTCACTATTGTTTTCGCTGCACTTGGATTCATGTCGCCTGCC TCAAGAGGAATGCTACTAACCGGGATGATTATTCTCTATCTTTTCTTGGGTATTGTTGCTGGGTACGCTGGTGTTCGGCT CTGGAGAACTGTCAAGGGGACTTCAGAAGGATGGAGATCACTTTCCTGGTCCATTGCATGCTTCTTCCCGGGTATTGCTT TTGTTATACTCACAGTGTTGAACTTCCTTCTCTGGAGCAGCAACAGCACTGGAGCCATCCCAATCTCTTTGTATTTTGAG CTCTTGGCTCTCTGGTTCTGCATCTCGGTTCCTCTCACCCTATTTGGAGGATTCCTAGGAACACGAGCAGAAGCAATCCA GTTCCCAGTGAGAACCAACCAGATTCCAAGGGAAATCCCAGAACGGAAATACCCGTCATGGCTTCTAGTCCTTGGAGCAG GAACCCTTCCTTTTGGAACGCTCTTCATAGAACTGTTCTTCATCTTCTCCAGCATATGGTTAGGGAGATTCTACTATGTA TTTGGGTTCTTACTCATAGTTCTGCTTCTTTTGGTAGTCGTGTGTGCAGAAGTATCAGTGGTCTTGACCTACATGCACCT CTGCGTTGAAGACTGGAGGTGGTGGTGGAAAGCCTTCTATGCATCTGGTTCAGTCGCATTGTACGTTTTCGCTTACTCCA TCAACTATTTGGTCTTTGATCTACAAAGTCTGAGCGGACCCGTCTCAGCTATGCTTTACATTGGCTACTCTCTGCTTATG GCAATTGCAATCATGCTCGCCACTGGAACCATTGGCTTCCTCACCTCCTTTTACTTCGTCCACTATCTCTTTTCCTCTGT CAAAATCGATTGATACCTTGACGACAAAATCTCAGCCGTTGATCTCTGCTTTCTCTAAAGCAATTTCAGGTGTTGTTATT TTTTTGTCAGTACTAGACTTGTAGAGTCCACT ..................N......................N.............N........................ ......................................................i......................... .............................N.........................N...............N........ ................................................................................ ................................................i............................... .......N.........N...............N...................N.....N.................... .............................i..........................................N....... .................................................N..N........................... ..............N.....N........N....i.............................i............... ...........................N.........................................N..N....... ...i..............N...N......................................................... .....N....................N..............................i...........N....N..... ........N............................................N.......................... ..............................................N...............................N. ..N...............N...................N...................i.............i......i .........N...................i......................................N........... .........N..............N....................................................... ..............................N..........................N...................... ................................................................................ ................................................................................ .........................................................N...................... .......................................................i...................N.... ........................................................................N....... ......................................N......................................... ..................................................N..........................i.. ............i................................................................... ................................................................................ ................................ Pos Score Pred ------------------------ 19 0.238 - 42 0.223 - 56 0.292 - 135 0.525 Yes 190 0.278 - 216 0.280 - 232 0.449 - 369 0.739 Yes 408 0.265 - 418 0.093 - 434 0.172 - 454 0.101 - 460 0.202 - 510 0.547 Yes 553 0.404 - 610 0.136 - 613 0.161 - 655 0.154 - 661 0.210 - 670 0.236 - 675 0.830 Yes 705 0.736 Yes 748 0.044 - 790 0.198 - 793 0.230 - 804 0.759 Yes 819 0.497 - 823 0.129 - 886 0.301 - 907 0.180 - 938 0.636 Yes 950 0.306 - 955 0.176 - 969 0.450 - 1014 0.263 - 1087 0.370 - 1119 0.406 - 1123 0.132 - 1139 0.410 - 1159 0.090 - 1179 0.741 Yes 1193 0.513 Yes 1200 0.661 Yes 1210 0.282 - 1230 0.670 Yes 1269 0.463 - 1290 0.447 - 1305 0.386 - 1391 0.491 - 1418 0.297 - 1658 0.449 - 1736 0.703 Yes 1756 0.260 - 1833 0.232 - 1879 0.186 - 1971 0.296 - 1998 0.503 Yes 2013 0.511 Yes

Explain the output -- Processing locus 226: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAACAACAACAACATCAATGGCTTCCCAAAAACATTGCCCAAACCCCAAAGTTCTCAAGTAAATCTGAGAAAGAAAAG AACATCTCATGATCTGAATAACAAAGAGCGAGAGACAGAGAGAGAGGCTTTATCTCTTTTCCTCAAGTGAGAGCAAAAAT GGTGGACGTGATACCAAAACATATCAAAGATGCGTGGGACAGATGGAACATTAGAGGAACAATCTTTCTAAGTCTTACCC TTCAAGCCTTTCTCATTTGCTTCTCTCCATTAAGGAAACGAACACCAAGAAGGCACTTGATTATCGTCATTTGGTCCTCT TATCTCTTAGCAGATTGGTCTGCTAATTTCGCTGTTGGTCTCATCTCTAAGAACCAAGGTAAAGATCTCAAACCTGATGA CCCTCCACAGGACAAAAAGCTCATGGCTTTATGGGCACCATTCTTGCTCTTACATCTTGGTGGACCAGACACTATAACAG CTTTCGCGCTTGAAGATAACGCTCTTTGGCTCCGAAATGTTTTTGGGCTTGTCTTTCAGGCAATTGCTGGTGTCTATGTG GTTCTCCAGTCTCTGCCCAATAGCCTTTGGGTAACAATACTCTTAGTGTTCATTTCTGGGACTATAAAGTATCTGGAAAG GACAACGGCTTTGTATAGTGCAAGTTTGGACAAGTTTAGGGATTCTATGATTCAAGGACCAGATCCAGGACCAAATTATG CGAAGCTCATGGAGGAGTACAAGGCCAAGAAAGAAGCAAAATTACCCACCAAGATCATTCTGATTGATGAGCCTGACAAG GAACATAGGCCTAAGAAGTTAGAGCATCCATCCCTGGCCTCGGAGACAAAAAGAAAAGAGTTGACTCATCTTGAAATTGC TCAGTACGCTTACAAATTCTTCAACACATTCAAGGGCCTTGTAGTCAACCTTATCTTCAGCTTCAGGGAGCGTGACCAGA GCATAGAGATCTTTCAGAACTTGGAAGATCCAGAGGAAGCTTTAAGAATCATTGAGATTGAACTCGGCTTTCTCTACGAT GCTCTATTCACCAAGAACGCGGTTCTTCATACTGTTTTGGGAACTGTCTCACGAGTTGTTGCTTCGGGTTCCCTTGTGGC TGCATTCATCATCTTTCATAAGATATCTAACAAAGGCAGAGACTTTCATGGAGCAGATGTCGTGATTACCTATATTCTGT TTGCAGTTGGCCTAGTTTTGGATTTCATATCCATCCTTTTGTTTCTGTTCTCAGACTGGACATGTGCTGCACTTAGTAGC TTGAAGGACGATCCGGATGAGCCTCTATCATGGAAAGATAGATTTTTCAACTGTTTACTTGAATTCAGAAAGCTGCGTTG GAAGATGCAAGAGTGCCACAATAAAGGTGAGCATAAGTGTACAAAAGAAGGTGAGAAACCGTGCATCAAGGAAGGTGATG ATAAGTGTTCAATGGTACACGTGCATCACATGCTCACGACGCCATTCTTCTTCCGGAGGTGGTGTGGAAGCATCAACGTA TTCAACTTTCTAGCATATGCCACAAAAAAAAGACAAAAGAGGATCCACGATGCCGGAGATGAGTTCCGCTCCTTATGGAT CATCATAGCTTTCCCGTTCAAAATAATCAATCTCATAATCCAGAAAGTTTTTGGATGTATTGTCGAGGTGATCAATAATG TGCACAGATGGATCCGCAGCATAGTCAATACGTTATCAAGAAACCACCCTTGGGCTCGTTGTACTATTTACCCTCTCTAT ATATTGTTCCTATTCCACATCCCTAATTTCATCAAATATTTGTGGAATGTTCTCATTGAGTTCTTAGGCATCCCGGATGC GCTTGATCTGGTCGAGAAGACGTTATTTGTACACGGAGAGCCCATGACAAAAGAATTGTGGAAGTTCATCTTCGAAGAGC TGAAAAACAAATCAAAGTATGGAGACAGTCCTGAAAATGCCAAAAGGATATCTTTGGCTAGGGGAGAATGGGCTTTAAGG GACAACCTACCAGAGGATGCAGAACGTGAAAAACTGGTACGTTATGTCACAAAGGTCGATTATGATCAGAGTCTTTTGAT GTGGCACATCGCAACCGAGTTCTGCTACCAAGAACATAAGAAAGAGACTATCCCAGAGGGTTATGATGAACAATGCAAGC AAAATCATCTCAGACTACATGATGTATCTCCTGATCTTGCAACCCAGTCTGATGTCCGAGGTTGCTGGAATTGGGAAGAT CAGATTCAGAGACACATTGGCGGAAACTGACAAGTTTTTCGAGAGGAAACGAATCTATAATGTGGGCGTGAATTTAGCCA CAGAGTATATACTGGATGTCAATAGTGAAATTGAACCAGTGGACGTCAA ...................i............................................................ ........N.....................................................................N. .............................i............i..................................... ................................................................................ ............................................................................N... ......................N.......i................................................. ....................................N......................................N.... ................................................................................ ..............................................N..............................N.. ........i.........................................................N............. ................................................................................ ................................................................................ ..............................................................................N. ................................................................................ ...............................................N........N....................... .............................................................N.................. ................N............N.................................................. ....i........................................................................N.. ...........N.................N.................................................. ................N................................i........N...............N..... ......................................................i......................i.. .......i........................................................................ ..............................................N.............................N... ...........................................N.................................... ..................i.................N..............................i............ ................N..........................N.................N................i. ..............................................................N..N......N....... ..................N..i.............................N............................ ...........................................................N.................... ...............N................................. Pos Score Pred ------------------------ 20 0.821 Yes 89 0.361 - 159 0.436 - 190 0.567 Yes 203 0.664 Yes 397 0.284 - 423 0.476 - 431 0.584 Yes 517 0.196 - 556 0.117 - 687 0.277 - 718 0.245 - 729 0.548 Yes 787 0.224 - 1039 0.174 - 1168 0.243 - 1177 0.152 - 1262 0.400 - 1297 0.338 - 1310 0.464 - 1365 0.584 Yes 1438 0.123 - 1452 0.378 - 1470 0.339 - 1537 0.254 - 1570 0.579 Yes 1579 0.141 - 1595 0.404 - 1655 0.568 Yes 1678 0.737 Yes 1688 0.610 Yes 1807 0.367 - 1837 0.225 - 1884 0.420 - 1939 0.592 Yes 1957 0.443 - 1988 0.636 Yes 2017 0.215 - 2044 0.128 - 2062 0.489 - 2079 0.507 Yes 2143 0.371 - 2146 0.317 - 2153 0.472 - 2179 0.326 - 2182 0.506 Yes 2212 0.481 - 2300 0.073 - 2336 0.031 -

Explain the output -- Processing locus 227: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCATATTCCAGAGGCCAATGTCACGTTGTACAACGACTGTGTCCACTGGTGCTTGCCCGGTCCTATCGACAACCTCAATG ACTTCTTGCTGGCTATGCTAAAGAGAGAAGAAGACAAAGGATTCTTGGCGCAAGTTCGAAAAATGTTGTCCTAATGACCA AGCTAAATCAAGTTTTTTGACTTTCTGTCTCCCTCAGGTTTATTTATTATATTTCAGGAAATGGTCAAATGTTGTGCCTA GTTATTGGATGACCACGAGCTCACCATTATTCACCATATATACATACATATTTATGACAAGACCAGACACCATTGTCTTG AAGCAGACAATGGAGCTTCCTTTGTTTGCAATCCTTCAAAGAACACCAAGAGTTGCAATTACATTAGCATTTGTTCTATT TGTCCTCACAATAGTTCCTGCTTTATATACTCTCCTTGCCGATCCAATCTTACCTCCATCAATCTCATCTTTCGAGACTG ATCATACACGTCTCAGTCATTTGAATTCTTCATCAAATCAGATTCCTTCACCCGTGAATGGTTCCATTCCCACGCCACCC TACCATACGCCTTCAAAACATCGGAAGTCTTCTTTCCATCGGATTCCTAAACCCAAGAAAGGTCCCATTTCCTCTCCAAC CGATCATATACCTTTAAGACAACGGAGTTCTTCATTCGATCAGATTCCTTCACCCATGAATAGTCCCGTTCCCGCTCCAC CACATCGGAATTCTTCAGCTGATCAGAGTCCTTCACCTGTGAACGGTCCCATTCCCGCTCCACTCAATCACACGTCCTTA AGACATCTGAATTCTTCATCTGATGATCATTCATCCCCAGTCACCACATCACCATCAAGGACGCGGATCAGAGATGATGA GCAAATGTGTGACCTTTTTACCGGGGAGTGGGTACCAAACGAAGAAGCTCCATACTACACCAACACGACATGCTGGGCAA TACATGAGCACCAGAACTGCATGAAATATGGGAGACCTGATACCGGATTCATGAGATGGAGGTGGAAGCCGGAGAGTTGC GACCTTCCCATCTTTGATCCTCAAGAGTTCTTGGAAATGGTTAGAGGGAAAGCCATGGGGTTTGTTGGTGACTCCATTAG CAGAAACCAAGTCCAATCTCTCTTGTGCCTTCTCTCTAGGGTGGAATACCCTGAAGACATTTCTCCTTCACCAGATACGG ATTTCAAAGTATGGAACTACACATCCTATAACTTCACTCTTCATGTCATGTGGTCACCATTTCTAGTGAAGGCTACTAAA CCTGATCCGAAGTCCAACTTCTTCAGCCTATACCTCGACGAATATGACACGAAGTGGACGAGTCAGCTCGACCAACTCGA CTACCTGGTTATATCATCCGGCCACTGGTTTTCCCGGCCGGTTATTTTCTATGAAAACCAACAAATCTCTGGGTGCCAAT ACTGTGCGTTACCAAACACGACTGAATTGCCTTTGACCTATGGATACAGGAAGGCTTTAAGAATATCTCTAAAAGCTATA ATCGAGAATTTCAAGGGTCTGGCATTTTTAAGGAGTTTTTCTCCTCAACATTTCGAAGGTGGGGCTTGGAACGAAGGAGG GGACTGTGTACGGACACAACCTTACAGAAGAAACGAGACTATACCCGAGGCAGATCTCAAGGTTCACGACATTCAAAGGG AGGAATTTAGAGCTGCAGAAGAAGATGGGATGAAGAAGAGTGGTTTGAGACTGAAGTTAATGGACACGACACAGGCAATG CTATTGCGACCAGATGGGCATCCTGGGAGATATGGACATCTACAGAATCCAAATGTGACTTTAAGAAATGACTGTATTCA TTGGTGCCTGCCTGGACCTATCGATACTCTGAATGATATATTGCTACAAATGATGAAGACAGATAACTAGAAAGCATCCC TCAAGAATGAGTAGTGGAGACATATATCTAATATCAAGAAAAATTGTAAAGAAAGATTCATGTAGGAGTCTCAGTATTCA ATTGCCATA .................i...........................................................N.. ..............N...............................................N..........N...... ............................................................N.......N........... ........N............................................N.......................... .........N...................................................................... ................................................................................ .........................................................N...................... ................................................................................ .......................................................i........................ ................................................................................ ......................N..................................................i..i... ....i................................................................i.......... ...N................N......N......................i....i........................ ....................................i.................i......................... ................................................................................ ..........i...............................N....i................................ ...........................................i.................................... ..................................................N............................. .......................................N........................................ ................................................................................ ................................................................................ ........................N....N.............................N.................N.. .............N.................N....................N..............N............ ................................N................N..i........................... ......N....................................................N.................... ......... Pos Score Pred ------------------------ 18 0.640 Yes 78 0.188 - 95 0.170 - 143 0.345 - 154 0.350 - 221 0.490 - 229 0.319 - 249 0.271 - 294 0.328 - 330 0.471 - 538 0.429 - 696 0.716 Yes 823 0.229 - 874 0.698 Yes 877 0.624 Yes 885 0.590 Yes 950 0.589 Yes 964 0.329 - 981 0.193 - 988 0.485 - 1011 0.703 Yes 1016 0.569 Yes 1077 0.695 Yes 1095 0.632 Yes 1211 0.574 Yes 1243 0.297 - 1248 0.517 Yes 1324 0.531 Yes 1411 0.425 - 1480 0.425 - 1705 0.302 - 1710 0.227 - 1740 0.374 - 1758 0.187 - 1774 0.235 - 1792 0.209 - 1813 0.217 - 1828 0.305 - 1873 0.147 - 1890 0.458 - 1893 0.546 Yes 1927 0.148 - 1980 0.049 -

Explain the output -- Processing locus 228: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACCACGTCACGCTTCCCAACCAAGTGACACGTGTCGCATCCACAACTCGTCAAAACACAAACACCAAAAAGTAGATGAC GTTACAAAGCATCATCCGTAAATGGCGGCTTCCTCTCTCTCGCTCTCTTCTCCTCTCTCAAACCCACTTCGCAGATTCAC TCTTCACCATCTTCACCTCTCAAAGAAACCCCTTTCTTCCTCTTCTCTGTTCCTCTGCTCCGCCGCAAAAATGAACGACC ATATCCACCGTGTTCCGGCTCTCACCGAGGAAGAGATTGACTCCGTCGCCATAAAAACATTTGAGAGATACGCATTACCT TCTTCTTCCTCCGTTAAGAGAAAGGGGAAAGGAGTAACGATTCTCTGGTTCAGAAACGATCTCAGAGTTCTTGACAATGA CGCATTGTACAAAGCTTGGTCTTCCTCTGATACCATTTTGCCTGTTTACTGTCTCGATCCTCGTCTCTTTCACACTACTC ATTTCTTCAACTTCCCTAAAACCGGAGCTTTAAGAGGCGGTTTTCTTATGGAATGTTTGGTTGATTTGAGAAAGAATCTT ATGAAACGAGGATTGAATCTTCTTATTAGAAGTGGTAAACCAGAAGAGATTCTTCCTTCTCTTGCTAAAGATTTTGGAGC TCGTACAGTTTTTGCGCATAAAGAAACGTGTAGTGAGGAGGTAGACGTTGAAAGACTTGTGAATCAAGGTTTGAAAAGGG TTGGAAATAGTACTAAGCTTGAGCTCATTTGGGGAAGTACTATGTATCACAAAGATGATCTTCCTTTTGATGTTTTTGAC TTGCCTGATGTCTACACTCAGTTTCGTAAGTCGGTGGAAGCTAAGTGTAGTATCAGAAGCTCTACTCGCATTCCACTATC TCTAGGGCCAACACCTTCTGTTGATGATTGGGGAGATGTTCCAACTCTTGAGAAACTTGGGGTTGAGCCTCAAGAGGTAA CCAGAGGAATGCGATTCGTTGGTGGTGAAAGTGCAGGAGTAGGCAGAGTATTTGAGTACTTTTGGAAAAAGGATCTATTG AAAGTATACAAAGAGACGAGGAACGGAATGTTAGGACCTGATTATTCGACGAAATTCTCTCCTTGGCTTGCCTTTGGGTG TATCTCTCCTCGTTTTATCTATGAAGAGGTCCAAAGATATGAAAAGGAAAGAGTAGCAAATAATTCAACATACTGGGTAC TTTTTGAACTAATATGGAGAGATTACTTCAGGTTTCTTTCAATTAAGTGTGGAAACTCACTTTTTCATCTAGGTGGCCCG AGAAATGTGCAAGGGAAGTGGAGCCAAGATCAGAAACTATTTGAGTCTTGGAGAGATGCCAAGACAGGGTATCCTCTTAT AGATGCAAACATGAAGGAGTTATCCACTACAGGTTTCATGTCAAATCGAGGCCGACAGATTGTTTGTTCGTTTCTTGTCC GGGACATGGGGTTGGATTGGCGTATGGGTGCAGAATGGTTTGAGACATGTCTATTAGACTATGATCCTTGTTCTAATTAT GGAAACTGGACCTATGGAGCAGGAGTTGGAAATGATCCACGGGAAGACCGGTACTTCAGCATTCCCAAGCAAGCACAAAA CTATGATCCAGAAGGAGAATACGTTGCGTTTTGGCTGCAGCAGCTACGTCGGCTTCCGAAAGAGAAAAGACATTGGCCAG GGAGATTGATGTATATGGACACAGTTGTGCCATTGAAGCATGGTAATGGTCCAATGGCTGGAGGGTCAAAGTCTGGGGGT GGCTTCAGAGGGAGTCACAGTGGGAGACGTTCTAGACACAATGGTCCTTAGAGAGAAACTATTCTTTACACGAACTTGTA TCTTATTTTTATTTCTGTGATTCTTCATTCTTGTGCACAAGGATCTCTGATTCTCTCGGTCTAAATTACA ...........................................................................N.... .....................i.......................................................... ......................................................................i......... ................................................................................ ............................................................................N... ................................................................................ ...............................................i....N........................... N............................................................................... ................................................................................ .........................................i............N..............N.......... .......N........................................................................ .......................N...........N............................................ ........i....................................................................... ...........................N.................................................... ....................N.................N......................................... .............N.................................................................. ....N..................................................N........................ ..N.......i..........................N.......................................... .....i.................i..........N...........N.............i.................N. .............i.................N................................................ ..N............................................................................. ........N.....N........................i.....N.......i.......................... ........................................N....................................... ...................................................................... Pos Score Pred ------------------------ 76 0.439 - 102 0.717 Yes 231 0.891 Yes 397 0.497 - 528 0.550 Yes 533 0.248 - 561 0.494 - 762 0.506 Yes 775 0.232 - 790 0.125 - 808 0.154 - 904 0.289 - 916 0.231 - 969 0.571 Yes 1068 0.450 - 1141 0.469 - 1159 0.492 - 1214 0.374 - 1285 0.307 - 1336 0.173 - 1363 0.135 - 1371 0.515 Yes 1398 0.194 - 1446 0.525 Yes 1464 0.675 Yes 1475 0.105 - 1487 0.120 - 1501 0.510 Yes 1519 0.385 - 1534 0.564 Yes 1552 0.492 - 1603 0.375 - 1689 0.447 - 1695 0.331 - 1720 0.774 Yes 1726 0.455 - 1734 0.537 Yes 1801 0.103 -

Explain the output -- Processing locus 229: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAAAAATGGTTTTTTTAATTACTGCTCTTTTTGTTCATTGTCAATATCCTTACTCGTGCATGCATTAATAAAAATTAAA ATTGGGGCCCTATAGTTTTGATCATCAATCGGTAGGTGTATGTCTCATGCGTTCATGGTGTTGAACCGATCATCTCTGTG TGTGTATATATATAGAGAGACAGACACTACTCTTCAATCATTCAAACAAGAAATCACCAAACACACATCTCTTCTGCACT CTCAAAACACTATGGATACTTCCCTCTTTTCTTTGTTTGTTCCAATCCTTGTTTTCGTTTTTATCGCTCTTTTCAAGAAA TCAAAGAAACCAAAATATGTAAAAGCTCCTGCACCAAGTGGTGCATGGCCCATCATCGGCCATCTTCACCTTCTCGGTGG CAAGGAACAGCTTCTCTACCGAACCTTAGGAAAAATGGCTGACCACTACGGTCCAGCCATGTCGCTACAACTTGGGAGCA ATGAAGCATTTGTTGTGAGCAGTTTTGAGGTGGCTAAAGATTGTTTTACTGTGAACGACAAGGCCTTGGCTTCACGTCCT ATGACTGCAGCTGCAAAGCACATGGGTTACAATTTTGCTGTTTTCGGGTTTGCCCCTTATAGCGCTTTCTGGCGTGAGAT GCGTAAAATCGCAACCATCGAGCTTCTTTCTAACCGGCGGCTTCAGATGCTCAAGCACGTTCGTGTTTCTGAGATCACAA TGGGTGTGAAAGATTTGTATTCCTTGTGGTTCAAGAATGGCGGTACAAAACCAGTAATGGTTGATCTAAAGAGCTGGTTA GAGGACATGACTCTGAACATGATCGTGAGAATGGTGGCAGGAAAACGATACTTTGGAGGCGGAGGCTCAGTATCGTCGGA GGATACTGAAGAGGCAATGCAATGCAAAAAGGCCATCGCAAAGTTCTTTCACCTCATCGGTATATTCACTGTGTCAGATG CTTTTCCGACACTAAGTTTTTTTGATTTGCAAGGACATGAGAAGGAGATGAAGCAAACGGGAAGCGAATTAGATGTGATC CTTGAAAGATGGATTGAAAACCATCGACAACAACGCAAATTTTCAGGAACGAAAGAGAATGATTCAGACTTCATCGACGT TATGATGTCGCTTGCGGAACAAGGAAAACTCTCGCATCTCCAGTATGATGCAAATACTAGCATCAAATCTACCTGCCTGG CACTGATTCTTGGAGGAAGTGACACTTCAGCATCAACCCTTACATGGGCCATTTCTCTTCTTCTAAACAATAAAGAAATG TTAAAGAAAGCACAAGATGAGATCGACATCCACGTCGGCAGAGACAGGAACGTCGAGGATTCAGACATAGAAAATTTGGT GTATCTTCAAGCAATTATCAAAGAAACATTGAGATTGTATCCAGCTGGTCCTCTCTTAGGCCCTCGAGAGGCGATGGAAG ATTGCACGGTCGCTGGTTACTACGTTCCGTGCGGCACAAGACTCATAGTGAACGTATGGAAAATCCAAAGAGATCCGAAA GTTTATATGGAACCAAACGAGTTCAGACCAGAGAGGTTCATTACAGGAGAAGCAAAAGAGTTTGATGTGAGAGGACAAAA CTTTGAGCTGATGCCATTTGGTTCAGGAAGAAGATCATGCCCAGGCTCTTCATTGGCCATGCAAGTGCTTCATTTAGGTC TTGCTCGTTTCCTTCATTCATTTGACGTGAAAACTGTTATGGATATGCCTGTTGATATGAGTGAGAACCCTGGCTTAACC ATTCCTAAAGCCACGCCTCTTGAGGTTCTGATCAGTCCACGTATTAAGGAAGAACTTTTTGTGTGATTAACTATTCCAGA AACATATAACTTCAATACGGTTATTGAAGGTGCATTTTGATGTGTGTGTGTTTTTCTTTTGTTGAATAAAACCTACGTTT GTTTG ......i.....................................................N................... .......................................N......N.......N......................... ................................................................................ ...........i.................................................................... ................i...........................N................................... ..................................i.......................i..................... N............................................................................... i....................i........................................................N. ..............................................N................................N ....................................N...................i....................... ......N...........i...........i................................................. ................i....N.......................................................N.. ....................................N..........N........................N....... ........i.................................................N..................... ...........................................N.................................i.. ................N............................................................... .........................................................................i...... .......................................................i........................ ......i.........................................................i............... ..........N.........................N.....................N..................... ......................................i.....i...........N....................... ................................................................................ .......................................N........................................ ..... Pos Score Pred ------------------------ 7 0.507 Yes 61 0.287 - 120 0.199 - 127 0.371 - 135 0.405 - 252 0.518 Yes 337 0.658 Yes 365 0.398 - 435 0.895 Yes 459 0.787 Yes 481 0.186 - 561 0.630 Yes 582 0.540 Yes 639 0.392 - 687 0.462 - 720 0.404 - 757 0.331 - 777 0.747 Yes 807 0.420 - 819 0.529 Yes 831 0.655 Yes 897 0.625 Yes 902 0.279 - 958 0.298 - 997 0.339 - 1008 0.441 - 1033 0.266 - 1049 0.643 Yes 1099 0.200 - 1122 0.384 - 1125 0.490 - 1165 0.170 - 1168 0.205 - 1244 0.270 - 1278 0.657 Yes 1297 0.206 - 1434 0.654 Yes 1496 0.571 Yes 1527 0.577 Yes 1585 0.579 Yes 1611 0.162 - 1637 0.260 - 1659 0.455 - 1719 0.648 Yes 1725 0.589 Yes 1737 0.487 - 1880 0.087 -

Explain the output -- Processing locus 230: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAATCTAAGAGTAACAATTAGAATCTAACCTTTATCATATCGAAACAAAATAGATTAGATTTTTTGCTAACTAAAAGTA TAATTTTCTTTTTGACTATGACATTTTAATATATTAATATATAAACTAAGTAAAATGAGTCTCTTTCACAAGACACTTTT GTCTTCTCTTTTAGAAAATCAGAACAGAGCCACTGCATGTTCTTCTTCTCAATCTCACTTTCTTCTTAAACCCATAAAAT TTGTTTCCATCGAAATATTTATTACCAATTATTTATATTTCTTAGATTTTGATTTCGTTTCCTTTTCTTCATCTTTGGTT CTTAAGATGAAGCTGAATAACCAGAAAGATCCAGCAGAGAAGCTAATGTGGGAACGGACTTTATCCGGATCCGGTTCACC GACCCGACCCGTATCCAAACTTATGGTAACGCTCCTCCTCCTCGTCTCCGCCACTTACGTTGTCTACACACTAAAGCTTA TCTCAAACTCACGTGCTTGTCAAGTGGAGCCATTCTCCGCCGTTGTTCGTCGTCTCAACGACATTGTCAACTCATCTCAA CCGTTGATTCTCTTCCACACAAATCAAACGGCTGTAATCAAATCATACGCCTCTCCACCACCTTCACCACCACCACCACC TCCGCCACCGCAAACCGGTTTCCAACACGTGGTTTTCGGTATCGCCGCGTCGGCAAGACTATGGAAACAGAGAAAAGAGT ACATCAAGATTTGGTACAAACCAAACCAAATGCGTAGTTATGTCTGGTTAGAAAAACCAGTTACTGAAGAAGACGAAGAA GACGAAATTAGCCTCCCGCCGGTGAAAATCTCCGGCGATACATCGAAGTTTCCGTATAAAAACAAACAAGGACACAGATC GGCGATTAGAATTTCAAGAATCGTTACGGAGACACTTAAATTAGGACTCAAAGATGTGAGATGGTTCGTTATGGGAGACG ATGATACAGTCTTTGTCGCCGAGAATCTAATCAGAGTTTTGAGAAAATACGATCATAATCAAATGTATTACATCGGCAGT TTATCGGAGAGTCATCTTCAGAACATTTATTTCTCTTATGGAATGGCTTATGGCGGTGGAGGATTTGCTATAAGTTATCC ATTAGCGGTGGCTTTGAGCAAAATGCAAGATCGGTGTATTAAAAGGTATCCGGCGTTGTACGGTTCCGATGATCGGATGC AAGCTTGCATGGCTGAACTCGGTGTTCCACTCACTAAAGAACTCGGTTTTCACCAGTACGATGTGTATGGCAATCTCTTT GGATTACTAGCGGCTCACCCGGTGGCTCCACTGGTTACGCTTCATCACCTCGACGTGGTCGAACCGATTTTCCCAAACAT GACACGTGTTGATGCCTTGAAGCATCTACAAGTCCCGGCAAAGCTAGACTCTGCTGGACTTATGCAACAGTCGATATGCT ATGACAAACGCCGCAAATGGACTGTTTCCGTCTCTTGGGGATTCGCGGTTCAGATTTTCCGCGGAATCTTTTCGGCTCGA GAGATTGAAATGCCGTCGAGAACTTTCTTAAATTGGTATCGACGGGCGGATTACACGGCATACGCTTTCAACACCCGACC CGTGAGTCGTCATCCGTGCCAGAAACCGTTTGTGTTCTATATGACATCAACCAGAGTTCATCGGGTGACAAATATGACAG TGAGCCGTTATGAAATCCACCGCGTGGCTCATCCCGAGTGTCGGTGGAAGATGGCTAATCCCAGCGACATTAAGACAGTC ATTGTTTACAAGAAACCGGACCCTCATCTTTGGGACAGGTCTCCTAGAAGAAATTGTTGTAGGGTGAAATCAAAGAAGAA CAATACTTTGGAGATTAGTGTTGCAGTTTGCAAAGAAGGTGAAGTGGTTGAAGTCGTGTAATAATATTCATGGTAAATTT TGTTCTGATTCAATTATACAGCAGATTAGTCAAATTACCACCTATTTAAATGATGGAAAATTTATTAAATAAATAAACAA ................................................................................ .................N....................................N......................... ....................................N........................................... ................................................................................ ......N......................................N.................................. ......................i......................................................... ................................................................................ ................................................................................ ............................................................i................... .............................i.........N........................................ ................................................................................ .....................................................N......N.........N......... N.............................................................N................. .....................................N....i......N.............................. ......................N.............................................N.......i... ........i...................................................N.....N............. ..............................................................................N. ...........N.................................................N.............i.... N...............N............................................................... .........N...................................................................... ........................................N................................i...... .........N........................................i............................. ................................................................................ .....................................................................N.......... .................................................N..N........................... Pos Score Pred ------------------------ 98 0.364 - 135 0.339 - 197 0.094 - 327 0.476 - 366 0.403 - 423 0.654 Yes 701 0.684 Yes 750 0.566 Yes 760 0.381 - 934 0.494 - 941 0.163 - 951 0.306 - 961 0.218 - 1023 0.426 - 1078 0.168 - 1083 0.615 Yes 1090 0.443 - 1143 0.487 - 1189 0.411 - 1197 0.588 Yes 1209 0.712 Yes 1261 0.353 - 1267 0.183 - 1359 0.460 - 1372 0.262 - 1422 0.317 - 1436 0.514 Yes 1441 0.288 - 1457 0.320 - 1530 0.499 - 1641 0.471 - 1674 0.705 Yes 1690 0.236 - 1731 0.708 Yes 1910 0.099 - 1970 0.061 - 1973 0.046 -

Explain the output -- Processing locus 231: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTAATATTTGTATTAAAATTTTATACTTCATAATAATATTTTTTTTTTTCCACTCTCTCTATTTTTTAAAAAAATAT ATATTTATGTTGATATATTAGTATGAGAGAAAAGAGTTGATGTGGGAGTCAAATATATGTTGTGATCCGTTAACTTCAAA GCAAATTACTGTTTATTCACAGAAGCTTGGACGCGTACGGCTTCGATTTCCTTATATATATATATTACACATAAATCTGC CGTCTTGGAGATTGGAAACATATCTCAAGCTTCTTCTTCGTGTTACGCACTTTCTTCTTCTTCTTCTCTCTCTCTCTTTC TTCTTCGTCTTCGATCACAGAAGACAGAATTCAATGTCGTCGACCTACAAGCTCAATCACACTGATTCATTTGCAAATGG AGATGCAAAGAGTTTGTTGCCGAACCCAGAGAATGGTTTCACTCATTTGACTTCACTTGCTCAATCAGAGAAGGCTGTTC AGGAGCTTCTTCTTCAACAGACTCCTATGCAGGCTACTGACGACCATCTCGTTGAGTTCTCTGAGGCTTTGAGAACTGTT GCAAAGGCACTAAGGGGAGCTGCTGAAGGAAAGGCGTTGGCTCAAGCTGAGGCTGCTGAATGGAAGCGTAGATACGAGTT AGAGAGGTCTAAGAACGTGGAGTTGCAGCATAAAGAACTGTCAAATGGAGTGTGTGCCGATGAATCTAATGGTCAAAGGA TGGAGCACTTAGCAAAGTCTCCGCGGCTTTATGCTCAGGAAATCAGTTCTAATGGCATGGAGACTATTTGCTCTCATGAA GTGCTCCAAGATGGTGGATTTAACAGTTTTAATAACAAATTGAAGAGGAAGGCATCATTTAAACTTTCATGGGGATGTAA GGGGATGGCTAATGATCAACACAAAAAAGAAATTGTTTCGTTTGAGAGAGGGAATATCAGCACAGCAGAGCGCAGTAGTA AACAGATTTCACTGACATGGGAGTCTGATCCACAAACTGTACTTATTATCACTAAGCCTAATTCAACTTCCGTGCGAGTT CTCTCTGTGGATATGGTCAGATGGTTGAGAACGCAAAAAGGACTAAATATTTATGTGGAACCTCGAGTAAAGGAAGAACT CTTATCAGAATCAAGTTCCTTCAACTTCGTACAAACTTGGGAAGATGACAAGGAAATTTCACTTCTACACACAAAGGTTG ACCTTCTTATAACTCTTGGTGGGGATGGCACAGTGCTATGGGCTGCATCGATGTTTAAAGGACCAGTGCCTCCAATTGTT CCATTCTCCATGGGATCTCTTGGATTTATGACTCCTTTTCACAGCGAACAATACCGAGATTGTCTTGAAGCGATTTTGAA GGGTCCTATCAGTATAACACTACGACACAGGTTGCAGTGTCACATCATCAGAGATAAAGCAACACATGAATATGAACCAG AGGAGACTATGCTTGTTCTGAATGAAGTCACCATTGACCGTGGAATATCTTCTTACCTCACAAACCTTGAATGCTACTGT GACAACTCATTCGTCACATGTGTGCAAGGCGATGGACTTATACTGTCTACAACATCTGGTAGCACCGCGTATTCACTTGC TGCTGGAGGGTCAATGGTCCATCCTCAGGTTCCTGGGATCTTATTCACGCCTATCTGTCCGCATTCTCTATCATTCCGCC CACTAATATTACCGGAACACGTGACAGTGAGAGTCCAAGTGCCATTCAACAGCAGAAGCTCCGCTTGGGTCTCCTTTGAT GGGAAAGACCGGAAACAGCTTGAAGCAGGGGATGCACTAGTGTGTAGCATGGCACCATGGCCCGTCTCAACCGCTTGTCA AGTTGAATCCACTAACGACTTCTTACGCAGCATCCACGATGGTCTTCACTGGAACCTAAGAAAGACTCAATCTGCTGATG GACCTTAGAAACAAATTCCTCCAGCTTTTTTCTCTTTCATTCTTAACCAATAACCATATATAGTCTCGACGTCTATCTAT CTATCTATATGCATATAGATAAATGTG ................................................................................ ......N...............N................N................N....................... ................................................................................ ................................................................................ .................................i..........................................N... ..N.............................N............................................... ..........................i..................................................... ...........................................................N.................... ............................................N..............N........N..........i ..............................N....................N....N..................N.... ..........i.........................................................N.....N..... ....i......N.................................................................... ................i............................................................... ............N.......N...............................N........................... ............................................N................................... ........................N............i............N............................. .........i.................N.................................................... .................................................................i.....N........ ........i............N................................................N......... .................i.............N................................................ .............N.................................................................. ..............................................................................i. ...............................N................i.......N....................... ......................................i......................................N.. ................................................................................ ........N.............N.... Pos Score Pred ------------------------ 87 0.154 - 103 0.248 - 120 0.323 - 137 0.303 - 354 0.661 Yes 397 0.237 - 403 0.167 - 433 0.315 - 507 0.752 Yes 620 0.393 - 685 0.375 - 700 0.209 - 709 0.303 - 720 0.603 Yes 751 0.178 - 772 0.443 - 777 0.344 - 796 0.208 - 811 0.548 Yes 869 0.268 - 875 0.392 - 885 0.519 Yes 892 0.234 - 977 0.588 Yes 1053 0.263 - 1061 0.473 - 1093 0.320 - 1165 0.475 - 1225 0.430 - 1238 0.585 Yes 1251 0.442 - 1290 0.587 Yes 1308 0.170 - 1426 0.641 Yes 1432 0.359 - 1449 0.558 Yes 1462 0.415 - 1511 0.478 - 1538 0.512 Yes 1552 0.279 - 1614 0.479 - 1759 0.601 Yes 1792 0.310 - 1809 0.633 Yes 1817 0.319 - 1879 0.556 Yes 1918 0.391 - 2009 0.051 - 2023 0.072 -

Explain the output -- Processing locus 232: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACACTTAAACAAAAGCATTTGTTTCATGGCTATAAATAGCTTATTCCTCATCATAGATAAGAAAAAACCTTTTCGAACT CAAATAATTTCTCCAAATTGAGATTTAAAAAAAAAAATGCTTGACTCCACCCCAATGCTCGCGTTTATTATAGGTTTGCT TCTTCTTGCATTAACTATGAAGCGTAAGGAGAAGAAGAAAACCATGTTAATTAGCCCTACGAGAAACCTCTCTCTCCCTC CCGGGCCGAAATCTTGGCCTTTAATCGGAAACCTACCGGAAATACTAGGGAGGAACAAACCGGTGTTCCGGTGGATACAT TCTCTCATGAAAGAACTCAACACCGATATTGCATGTATCCGTCTTGCGAATACTCACGTGATCCCCGTGACATCCCCGAG AATTGCAAGAGAGATTCTGAAGAAGCAAGACTCCGTTTTCGCCACTAGACCGCTAACGATGGGCACGGAGTACTGCAGCC GCGGGTACTTGACCGTTGCGGTGGAGCCACAAGGAGAGCAGTGGAAGAAGATGAGGAGAGTGGTGGCATCTCACGTGACG AGCAAGAAGAGCTTCCAAATGATGCTACAAAAGAGAACCGAAGAGGCTGATAACTTAGTCCGGTACATCAATAACCGTAG TGTCAAAAACCGTGGTAATGCTTTTGTGGTTATTGATTTAAGGCTTGCGGTACGGCAATACAGTGGAAATGTAGCTCGGA AGATGATGTTTGGTATAAGGCATTTTGGTAAAGGAAGTGAAGATGGATCGGGACCAGGGTTGGAAGAGATTGAACATGTG GAATCTTTGTTTACGGTTTTAACCCATCTTTACGCCTTTGCATTGTCAGATTATGTCCCGTGGCTAAGGTTCTTGGACTT GGAAGGCCATGAGAAGGTTGTGAGTAACGCAATGAGAAATGTAAGTAAGTATAACGACCCTTTTGTTGATGAAAGACTCA TGCAATGGCGAAATGGGAAGATGAAAGAACCTCAAGATTTTCTTGACATGTTTATAATAGCTAAAGACACTGACGGGAAG CCTACTCTGTCGGACGAAGAGATCAAAGCACAAGTGACGGAACTAATGTTGGCGACGGTTGATAATCCGTCTAACGCGGC AGAGTGGGGTATGGCGGAGATGATTAACGAGCCGAGCATCATGCAAAAAGCCGTGGAAGAGATTGATAGGGTAGTTGGAA AAGACCGTCTTGTCATTGAGTCTGATCTCCCAAATCTTAACTATGTGAAGGCTTGTGTGAAAGAAGCATTCCGGTTACAC CCCGTGGCACCGTTCAACCTCCCTCACATGTCCACCACTGATACTGTGGTAGACGGTTATTTCATCCCCAAGGGAAGCCA CGTATTGATTAGTCGTATGGGGATTGGGAGAAATCCTAGTGTGTGGGACAAGCCGCATAAGTTCGACCCTGAGAGACATT TGAGCACTAACACATGTGTGGATCTAAACGAGTCTGATCTGAATATAATATCGTTCAGTGCAGGACGAAGAGGTTGTATG GGTGTGGACATTGGGTCAGCCATGACGTACATGTTACTGGCTCGGTTGATTCAAGGATTCACGTGGTTACCAGTGCCTGG TAAGAATAAGATTGATATTTCAGAAAGCAAGAATGATCTTTTTATGGCAAAACCATTATACGCGGTTGCCACACCTCGTT TAGCTCCACATGTGTATCCAACCTAATGGAGAGCGTAAAGATTACTAGAAAAATGCATTAAACTGAAAACAAAAAGTGAA AGAAAAATGCACTATTAATTGTATGTCATGGTATGTTTCGTTTTT ..........................i..................................................... ....................................i.................N......................... ................N..........................N.................................... ................................................................................ ......i.........................N............................................... ..........................................................i..................... ..................................................N............................. ..................N..N.......................................................... .................N..................................................N........... ..N..N....................................N................................N.... ....................................................N........................... ........N......................N......N.............................N..........i ....i.......N.......i..........................i................................ .............................................i.................................. ..........i........i....................N....................................... ..........................................N..................................... ...........................i.................................................... ................N............................................................... .............i...............................................................N.. .....................N........N................................................. ................................N..........N.................................... .........N...............N..........................N........................... ......N...............N....N....N............ Pos Score Pred ------------------------ 27 0.593 Yes 117 0.837 Yes 135 0.315 - 177 0.446 - 204 0.286 - 327 0.644 Yes 353 0.229 - 459 0.776 Yes 531 0.372 - 579 0.336 - 582 0.262 - 658 0.173 - 709 0.165 - 723 0.481 - 726 0.436 - 763 0.266 - 796 0.293 - 853 0.370 - 889 0.204 - 912 0.263 - 919 0.275 - 949 0.058 - 960 0.592 Yes 965 0.593 Yes 973 0.469 - 981 0.814 Yes 1008 0.501 Yes 1086 0.842 Yes 1131 0.826 Yes 1140 0.606 Yes 1161 0.472 - 1243 0.407 - 1308 0.709 Yes 1377 0.415 - 1454 0.555 Yes 1518 0.431 - 1542 0.208 - 1551 0.374 - 1633 0.388 - 1644 0.237 - 1690 0.247 - 1706 0.218 - 1733 0.081 - 1767 0.067 - 1783 0.047 - 1788 0.034 - 1793 0.041 -

Explain the output -- Processing locus 233: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCTTTATCTATTTGTTTTGCTTTTTACTTATGGTTTTTCCGTGTTAATTATCTAAGAGTTAACGACAAAGAGGAAGAA GCGAAGTCAATGAATAAGTAAAAAAAAATTGAAAGAGGGAAACTTCTTTTTGAAGCAAGAGTTAACGACCTGCCGTTTCC ATACTTCAGAACACGAGGGGATACGTCGTCGTTTGCTGATTATTTTCCTCCAACATTTCAAACAAACAAAACGACATGCC GTTTTCAGCATACGAAGATAATACGTTGTCGTTTAACGGTCCTCCTCTGTTCCTTAAAATTAAAACCACTGAAACTCGAA AAAGCCTCACTCTTACTGAACCCTTTACTTGCCTCCAGTTTTTCCGGCCACCGGCAACGGCGAAACTCCGTTATTGTATC TAAAGTCATGCCTTTGAATTTCCCTCTCCGAATACGATTCTTCGCTCACTCTCTTTCGGGTACACATCTCTCTTATAATA CCTCTAGCTCCGTCCCTTTGCTCTTCCGGATATTCTCTTCTGGATTGAATCACTTTGAATTTGGGTCTCGCATAAACTTC TCAACCCGTCCTAACAGAGATCAACCAGAATTTGAGCGGCGGATTCGAGACGGAGGAGAAATTCGCGCGTCAAAGAGCTT GATCGAAGACGAGGAAGAGCTCAGCAATTGGGTAAGCGGTTTTAGAACCGGATCTTCGCGTGGAATTCTCAAGAGTGACG ATGAAGATGAGGAGGATCGTAGCAGAGGAAGAAACCAAGAGAAGAGAGGAATTCGCAACCAAGTTGATTCCTTTAGGAAT AAGCGTTATGGTGGTGATAGAGAAAGGGGATTCAATAGTCGGATTCAAGGAAAGAGTAGCGAGGCGTCATTTCGTGGGAG GAAAGAGACAAGCTTTAGTAGAGATAGAGAAGACGAGAAGGGTTTAAGGAAACGGGAAGATCTTCGTCTTGAAGACGAAA GTAGTGATGAGGATGTCAAGAGTTTAGTAATGGGAGATATTGGGGATTTGCTTAGTGAAGATGATGAGGAAGAGGACCAA GATTATGACTTTCTTAAGAAGAAAGCTGTTTCTGCTTTTGGGTTTGATAAGGAGAATGTTATAGAAGCGGATAAAACGAG GAATGCAAATGATTCTTATTTGACTAAGACAAGATTTGATCACTATCCATTGTCTCCCTTATCGCTAAAGGCAATCAAGG ATGCTGGATACGAGACAATGACTGTTGTTCAGGAAGCTACTCTCCCTATAATTCTCAAAGGCAAGGATGTCCTAGCTAAG GCCAAAACAGGAACTGGGAAAACTGTAGCGTTTTTGCTTCCATCAATTGAAGTAGTTGTCAAATCTCCTCCTACAAGCCC GGATAATAAGCGACCCCCAATCCTTGCGCTTGTGATATGCCCTACTAGAGAACTTGCCAATCAAGCTGCTACAGAAGCAA ACACCTTGCTAAAGTATCATCCATCTATTGGTGTTCAAGTTGTGATTGGAGGTACGAGACTTGGTTTAGAGCAAAAGCGT ATGCAAACAAATCCTTGCCAGATTTTAGTGGCTACACCTGGAAGGCTCAAAGACCATATCGAGAACACGCCAGGCTTTGC AACGAGGTTAAAGGGTGTGAAAGTCCTTGTGCTTGATGAAGCTGATCATCTTTTAGATATGGGCTTTCGCAAGGACATTG AGAGGATAATTTCCGCTGTTCCTAAGGAGAGACAAACTTTTCTATTTTCCGCCACAGTACCTGAAGAGGTACGCCAAATA TGCCTTGTTGCTCTAAGGCGGGATCACGAGTTTGTCAATTGTGTTCATGAAGGCACTATAGAGACGCATCAACAGGTCAG ACAAATGCACATGATTGCATCACTGGACAGACATTTCTCTCTTCTGTACACACTTCTTCGCGAACACATTATGGGTAACG TAGACTATAAGGTCATTGTCTTCTGTACAACGGCCATGGTTACAAAATTAGTGGCTGATCTACTTGGTGAGCTAAACTTA AACGTGAGAGAGATCCATTCTAGAAAACCGCAGAGTTACAGGACAAGGGTTTCTAACGAGTTCCGAAAATCCAAGGGTTT GATTCTCGTAACATCTGATGTATCTGCTCGAGGAGTTGATTACCCTGATGTGACCCTTGTTTTACAGGTTGGATTGCCAA AAGACAGAGAACAATATATACACAGACTTGGTAGAACCGGGCGAAAAGGAAAGGAAGGAGAAGGCATATTGTTGTTGGCA CCATGGGAGGAATACTTTCTATCATCTCTTAAAGATTTACCCATCACTAAGTCTCCTCTGCCATCAATAGACCCAGAAAC TGTAAAAAAGGTGCAAAAGGCGCTTTGCCATGTCGAGATGAGGAACAAAGAGGCGGCGTATCAGGCTTGGTTGGGTTACT ACAACTCACAGAAGATGATTGGAAGAGACAAAGACAGGCTTGTGGAGTTGGCAAACGAGTTTAGTCGTAGTATGGGACTT GACAATCCTCCAGCTATACCAAAACTTATCCTTGGTAAGATGGGTCTCAAAAATGTTCCTGGTCTTAGAGCCAAGTAAAT ATTCCTTCGTTTTTTATTTTTTGGCTTTGTAAGAAACTCCAATTTTTTTTGTTATTCCTTTAATTTTAAGGTTTCTTGCA GCAATTCATCATCTAAT ...............................N................................................ .........N...................................................................... ...........................................................................i.... ................................................................................ ................................................................................ .......N........................................................................ ................................................................................ ................................................................................ ................................................................................ N.....i......................................................................... .......N........................................................................ ................................................................................ ......N.....N................i..............................N..N................ ....N..................................................N........................ ..N.....N....................................................................... N................i................................................N............. ................................................................................ ....................................i........................................... ................................................................................ i............................................................................... ...................................N......................N..................... ...............................................................................i ..............................................N................................. ....N.....i...........................................................i......... ...................................i............................................ ................................................................................ .................i.............................N................................ ................................................................................ ..i............................................................................. .............................N.......N.......................................... ..............N........................................................N........ .......................................N............N........................... ................................................................................ ................. Pos Score Pred ------------------------ 32 0.332 - 90 0.470 - 236 0.722 Yes 408 0.413 - 721 0.446 - 727 0.519 Yes 808 0.385 - 967 0.079 - 973 0.133 - 990 0.799 Yes 1021 0.085 - 1024 0.118 - 1045 0.154 - 1096 0.145 - 1123 0.109 - 1129 0.145 - 1201 0.487 - 1218 0.785 Yes 1267 0.375 - 1397 0.567 Yes 1521 0.514 Yes 1636 0.133 - 1659 0.499 - 1760 0.786 Yes 1807 0.181 - 1845 0.350 - 1851 0.562 Yes 1911 0.788 Yes 1956 0.682 Yes 2098 0.529 Yes 2128 0.451 - 2243 0.650 Yes 2350 0.253 - 2358 0.306 - 2415 0.263 - 2472 0.332 - 2520 0.275 - 2533 0.183 -

Explain the output -- Processing locus 234: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAATGCATAATCATGCAAGCTGTACGTTAACGATCAATGGCAATTTCTTGTAAATTTGCAACACGCTCATGGGTAATTT CGTCTTGGTTTTGTTATATAAATACCCCATCAATTGCTCTCCAACATGCATTGTCTGAAACCAAACAAAGAAAATACACG AAGGAAGGAAAAGAAAAGTAAAAAGAAAAGCTTTCTGCAAAATCCAAACATGTCCGCCAAGATTTCAATATTCCAAGCTC TTGTCTTTCTATTCTACCGGTTTATCCTCCGGCGATATCGGAACTCTAAACCAAAATACCAAAATGGCCCTTCTTCTCTC CTCCAATCCGACCTATCACGCCACACATTGATCTTCAACGTAGAAGGAGCTCTTCTCAAATCCGACTCTCTCTTCCCTTA CTTCATGTTAGTAGCATTTGAGGCGGGAGGCGTAATAAGGTCATTTCTCCTCTTCATTCTCTATCCATTGATAAGCTTGA TGAGCCATGAGATGGGTGTCAAAGTGATGGTAATGGTGAGCTTCTTCGGGATCAAAAAAGAAGGTTTTCGAGCGGGGAGA GCGGTTTTGCCTAAATACTTTCTAGAAGATGTCGGACTCGAGATGTTCGAAGTGTTGAAGAGAGGAGGGAAGAAAATCGG AGTGAGTGATGATCTTCCTCAAGTTATGATCGAAGGGTTCTTGAGAGATTACTTGGAGATTGACGTTGTGGTCGGGAGAG AAATGAAAGTCGTTGGAGGTTATTATCTAGGTATCATGGAGGATAAAACCAAACATGATCTTGTCTTTGATGAGTTAGTT CGTAAAGAGAGACTAAACACCGGTCGTGTTATTGGCATCACTTCCTTCAATACATCTCTTCACCGATATCTATTCTCTCA GTTTTGCCAGGAAATTTATTTCGTGAAGAAATCAGACAAGCGAAGCTGGCAAACCCTACCACGAAGCCAGTACCCTAAAC CATTGATTTTCCATGATGGCCGTCTCGCGATCAAACCAACCCTAATGAACACTTTGGTCTTGTTCATGTGGGGTCCTTTC GCAGCCGCAGCCGCAGCAGCCAGACTCTTCGTCTCTCTTTGCATCCCTTACTCTTTATCAATCCCGATCCTCGCCTTTTC CGGTTGCAGACTAACCGTCACTAACGACTACGTTTCATCTCAAAAACAAAAACCAAGTCAACGCAAAGGTTGTCTCTTTG TTTGTAACCATAGGACTTTATTGGACCCTCTCTATGTTGCATTCGCTTTGAGAAAGAAAAACATCAAAACTGTAACGTAT AGTTTGAGTAGGGTATCTGAGATTTTGGCTCCGATCAAGACGGTGAGACTGACCCGTGATCGGGTGAGCGACGGTCAAGC CATGGAGAAATTGTTAACCGAAGGAGATCTCGTTGTTTGTCCTGAAGGAACCACTTGTAGAGAACCTTACCTGCTTAGGT TTAGCCCTTTGTTCACCGAGGTTAGTGATGTCATCGTTCCCGTGGCTGTGACGGTACACGTGACCTTCTTCTACGGTACA ACGGCGAGTGGTCTTAAGGCACTTGACCCGCTTTTCTTCCTCTTGGATCCTTATCCTACCTACACCATCCAATTTCTCGA CCCTGTCTCCGGTGCCACGTGCCAAGATCCTGATGGAAAGTTGAAGTTTGAGGTGGCCAACAATGTTCAGAGTGATATTG GGAAGGCGTTGGATTTCGAGTGCACAAGTCTCACTAGAAAAGACAAGTATTTGATCTTGGCCGGTAATAATGGAGTAGTT AAGAAAAATTAAAATTTGTTTTAGGATGATGAGTGAGTGATGAGTTCGGTTCTTTGGGTATGTAAGTAACCAATCGATTA TTTCATTTGTTTATTAATAATGTCTACGTAA ...N.........N.......................i...............................N.......... .............................................N.................................. .................................................i.............................. ...............................................................i................ ................................................................................ ....N..........................................................................N ......N....i..............N.....i............................................... ............................N.............i..................................... ........N................N...................................................... ..i................................i..................N..............N.......... ................................................................................ ................................................................................ ............N..i............................N....................N.............. ................................................................................ ................................................................................ .................................N.............................................. ................................................................................ ...........................N.................................................... ................................................................................ ................................N.............................N................. .....................................................................N.......... .........................N..N..........N...................N.................... ...................N........... Pos Score Pred ------------------------ 4 0.174 - 14 0.299 - 38 0.546 Yes 70 0.451 - 126 0.335 - 210 0.724 Yes 304 0.743 Yes 405 0.155 - 480 0.249 - 487 0.452 - 492 0.501 Yes 507 0.334 - 513 0.528 Yes 589 0.281 - 603 0.524 Yes 649 0.227 - 666 0.369 - 723 0.571 Yes 756 0.545 Yes 775 0.163 - 790 0.185 - 973 0.339 - 976 0.588 Yes 1005 0.368 - 1026 0.288 - 1234 0.209 - 1362 0.447 - 1468 0.305 - 1633 0.196 - 1663 0.292 - 1750 0.236 - 1786 0.077 - 1789 0.064 - 1800 0.099 - 1820 0.110 - 1860 0.067 -

Explain the output -- Processing locus 235: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAATCAATTATACAAATGACACAAATTTAATATTTGTCGTGATTGTCATTTTCAGGTTATTAACTTGCATGACCACACC ATGTCGTTTCTAGCATAATAATATAAGAGTAATTGTTGGAGACCTTTGTCTTCTCTTGATCTTCTCAGACGGCAGTTCAT AACAAAACTCCTAATTAAGAATTTGGTAGTTGGATCCCAGGTACTTTTTTATATGCACACGATTCTATTATACAATGGAC TCTGGCTGATCGAATTGTCCGGTGTACACGCAGGTTTGCATACCGATCCGGGGATGGATTTCTTCCATTCAAGAGAGAAG AGACATGGCAAAAGAGCAGGATTGTTTGGTCACAAAAGTGCGAGCAAGAGCAACCCGTCGAGCCCTCCTCATCTTGCTGA GGGTCGGTCTCAGCCGACAACTCATTTCGGCATGAAAAGATCGGAATCAGAGTACGCGTTTCCCATCTCCGATGAACAAA CCACTCACTGGAAACCACAACAGCAAGCCTCTGAACGCATCCCAAATTCCCACCAACGGCCTCCAGTCTACAGATATAGC ACGCCTGAGCGTCCTAGAGAGCCTGATGAGGATGTGACTCCAAGGAGCAACGGTTCTGGGAGCCCTTTTCGATCTGCCAG AACCCGCACGCCTGATCGTCGTAAAAGGTCTAGCGAGATAACCAAAGAGCTGTACGAGAGAATGTATGAGGCAGATGCCA ATGTGAGTCCATTTCACCCCTTCAGAAGCCGCTCTCCAGCTCCCTACAACACCCATGAACGTGGAAGAGACTACAGTAGA GAAAGATATGAAGCTGAAGGCAATGTAACTTCCCGGAACAGACCTTCAAGCCCATTTCATCCATCCCAAAGCCGCTCTCC ACCGCCACACGCAAGGACTCAGAGATACAACAACGGAAAAGATCATTTCGAGGGGATGTACGAGGCAGATGCCGATATCA CACCGCGTAACAGCCCTCCCATGAGTCCTGTTCATCAACACACAAGCTACTCTCCACCACCGCCGTTCTACTCATCCAGT GACGACGAAGACGACAACAATTCCACCTATCTCTTTCCAGAAATTGCCACTGGACGTCGTTCCAGGGGAGTTTCAGGAAG CAGCACGCCGGTTCACTACAAGTATCAGATCACATCGGTAGAGACTTACGAGCAGGAGAGGCAATTCGAGCCGCCGGAGC TGCCGGACGAGTCACAGAGCTTCACGATGCAGGAGATCGCCAGAATGCGCGGACTTCAAAGCTACGGAAAGGAAGAGATA CAATTAGCGATATCCGAGACTTACGTCTCCGTCGCCAATTACAAGGTACGGATGAGCATCGCCGCCACGCTCGAAGCGAT CATTGACAAACACGGTGACATCGCCGCCTCGTCGAAGTTACAATCTACCTCAACGAGGTCGTTTTATCTCGAATCCCTAG CCGCAGCAGTAATGGAACTGAAATCAACGGCGTTGAGAGATCTGACGAAGACGCGAGTGGCGGAAATCGCGGCGGTCGTG AAGGACATGGATTCGGTGAAAATCGATGTATCGTGGCTCAAAACAGCGGTGACGGAGCTAGCGGAGGCGGTGGAGTACTA CGGGAAGTACGACACGGCGAAGATTGTGAGGGAGGAGTGCGATAGGGAAATGACGGCGGGGAAAAAAGAGATGGAGGAGA TGAGGGAGGAGCTGCGGCGGAGAGAGAAGGAAACGAAGGAATGTAGAGAGAGAGTGACGGAGTTGGCGGGACGGCTGGGG CAGCTGGAGATGAAGGATGTGAGAGTGAAGAAGAATCTGGAGCTCTATGAGAGCAAGGTCCTTAAATTCGACGGTCATTC GGTTCTCGTTGACTGAACCCGACCTTTTGGAAATTTAATTAAACCGGTTTAGTGGAGTAAACCGCTTTGGAAAATGCTTA TCCAAGCATAATCACTCGGAGAAGTCTTTGTTTGT ................N....................................................N.......... N............................................................................... ....................................................N.....................i..... .....................................................N.......................... ....i........................................................................... ...............................N.......................................N........ ................................................................................ .........................N.....N................................................ .............................................................i...N........N..... i.....................................................i......................... .......i..............i......................................................... .......................................................i............i........... ....................N........................................................... ................................................................................ ................................................................................ ..........................i.................i................................... ...................................................i............................ ................................................................................ ...........i.................................................................... ......N..................N...................................................... .................................................i....................i........N ........................................i....................................... .........N......N.............................N................................. .........................................................................N...... ................................... Pos Score Pred ------------------------ 17 0.354 - 70 0.473 - 81 0.262 - 213 0.259 - 235 0.700 Yes 294 0.266 - 325 0.601 Yes 432 0.390 - 472 0.377 - 586 0.289 - 592 0.328 - 702 0.898 Yes 706 0.483 - 715 0.499 - 721 0.740 Yes 775 0.573 Yes 808 0.547 Yes 823 0.768 Yes 936 0.510 Yes 949 0.514 Yes 981 0.358 - 1227 0.603 Yes 1245 0.522 Yes 1332 0.542 Yes 1452 0.604 Yes 1527 0.470 - 1546 0.149 - 1650 0.622 Yes 1671 0.644 Yes 1680 0.411 - 1721 0.850 Yes 1770 0.398 - 1777 0.487 - 1807 0.135 - 1914 0.053 -

Explain the output -- Processing locus 236: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTTACGAACAGTACAGACAAATAGATAACCGATTAAAGGACCGGAATGTATGAAAAAAAAATCAATCTGGGCATTTTAA GAGAGACGCGGTTTAAGATAATGGGCCTATTCTTTTGATAAAGCCCAATGTAACTTTTATTTTTTTAACAAACAAAAGGC CCAAATAAATTCGCCGAAGAGAAAAATAAGAAAATTTGTAACTCTCATGGCGGAAGTGTCTCTGACAAAGAAGGAGATGA CGACAAAGGGAAAGAGCGAGAACTCAAAGAAGATGAAGACAGATATGGCTGATATGGTTCCAATCGCCGCCATGAACGAA GACCTTCTCCACAACATTCTCTTGAGATTGCCGGCAAAATCGTTTGCTTTCGCTTCATGTGTTAACCGATTCTGGAGCAG CGTCTGCAATCGAATTCTCTCTCGCCCCAAAATGATATCTGCTTTCTCTCGTAATCCTGATCAACTTAGAGCTGGAGAAG AAGTACTTGATAAGGTTTTGTCTGAGCCAATTCGACCACAGTTTGTGATCGCAAACATTACATGTGGGAACATGGAAGAA ACTTTGACACTGATAACAGAAAGAGTGGGATCAAGGGTTCCTATCATTGTATCTGTTGTTACTGGAATCTTAGGAAAGGA AGCATGCAATGACAAGGCTGGAGAGGTCAGACTGCATTCCACAAGTGATGATGAATTGTTCGATGTTGCAAATTTTGCTA TACTGTTGACGATTGGCTACTTACCAGGAATGAAAGTCGACATTATTCCTGTAATTCAGGCTAAAGGGGAATCTGGAGCA GAAATGGAAGACAAATTTGTGATGGATATTAGAAATTATATGTCCATGGTTTCAGGTCATGCTGCTGCACCAGCATGTCT TATACTCTTTGCGGAGGATACTCATGCCACAGAACCTGTTCTTCACAAACTGGATTATGCCATGCCTGCTGAGACTGTTA TTGTGGGTGGTCAAATTGGCGAGTTTTTACATAAACGTGGTAATGAACCAAGAAATGTTCAGTTGCAGAAAGATGATATC AGAGTTCTCGCTGGCTTAATATTTGCAAGAGATAGACACAGACCTGCTCAGGCTGAAAGAATTCAGTTTGACACTGCAAT ATCAAATGGAATGTCATCGGTTGATCTGAGGTACAAGGCGGCTAATGTCAATGTTAGCCTCGGCCCTAGTTGTCCTTCGA CGCTTCTGACTGCCAAAAGAAGAGGAGAAGCAGAGGTTCTTGATGGCGATCAAATTCTAGATGACATAGATAATATTCTA GAAAACTATATCTGGGAGAATGATTCATACCTCGGAGTAATAAAACGTAGAAAATACTCAATTGGTTTGGAGGAGAAGCC AAAAATCATGTCCTCCCTTGTATTTCATCAAGTGAATGGATCTGACGATCAGGATCTTTTAGTTGATGGTGCCGGAATCA AAACCGGCGACCAATTCCAAGTATACCTCCCTGATCTCAAAGTGGCTGAAGCAGCACTAAATGATGTTTCTGCTCAACTT AGAAATCTCAAGTCTAAGCCGAATAAACCGGAAGTCGTTGGAGGTTTTGCTTTCGTCGGTAGCTGTCGCGGTGATTCCTT CTTTGGTTGTCCAAACGCAGACAGCTCACCGTTTTTAGAGAACTTCCCGGAGTTACCGTTTGGCGGTATATTCTGCGATG GTGAAATCGGACGCAGCTTAATCTTGGAGGAGGGAGAGGAAAAGAAAGAAGTGAGCATCCAAAGATTTCTTCACGTTTAT AGTTCGGTATATCTTATTGTCTCGTATACATCTTAATATTAAGGTGTCTGTTTGTTTTATGCTTATTAAGAGAATGATAA GTTAACTACAACTAGGACTAAGGAGGCAATAACTCTTGCTTTGCTTTCCATCTCT ..............................................N...N............................. ....................N..........................N................................ ..............................................i.............................i... ................................N...........i........N.................N........ ........................................................N....................... ...............................i................................................ .............................................................i.........i........ ................................................................................ ...N....N......................................N..N...........N................. .............................N.................................................. ...i.................N.................i.....i............N...............N..... .......................N................................N....i.................. ..........................................N...........N.................N....... ................................................................................ .....i....i.................................N.....N............................. ..........................................N.................N................... ...................N............................................................ .......i...........................i.............................N.............. ............................................................N..N................ ................................................................................ .............................................................................N.. ................................................................................ ..........................................................N..............N...... ....................................................... Pos Score Pred ------------------------ 47 0.160 - 51 0.135 - 101 0.385 - 128 0.475 - 207 0.602 Yes 237 0.654 Yes 273 0.439 - 285 0.742 Yes 294 0.285 - 312 0.244 - 377 0.203 - 432 0.600 Yes 542 0.652 Yes 552 0.722 Yes 644 0.495 - 649 0.381 - 688 0.111 - 691 0.124 - 703 0.105 - 750 0.270 - 804 0.658 Yes 822 0.488 - 840 0.706 Yes 846 0.637 Yes 859 0.130 - 875 0.178 - 904 0.251 - 937 0.256 - 942 0.763 Yes 1003 0.205 - 1015 0.240 - 1033 0.213 - 1126 0.683 Yes 1131 0.631 Yes 1165 0.326 - 1171 0.238 - 1243 0.234 - 1261 0.281 - 1300 0.130 - 1368 0.689 Yes 1396 0.646 Yes 1426 0.185 - 1501 0.336 - 1504 0.223 - 1678 0.409 - 1819 0.056 - 1834 0.035 -

Explain the output -- Processing locus 237: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTGTTATGTTATTGCTAGAGAGTTTTAATTTAGATTTTGATAAATACGGCATTACTAAATGACGATGATTATGCAATTT TATTCTTAAAGTGAATCATAGTTGATGACATTATTTGCAGATTCTATGGATATGTATAACAACAATATAGGGATGTTCCG GAGTTTAGTTTGTAGCTCGGCGCCTCCATTTACAGAGGGACATATGTGTTCTGATTCGCATACGGCTTTGTGCGATGATC TGAGTAGTGATGAGGAAATGGAAATAGAGGAGCTTGAGAAGAAGATCTGGAGAGACAAGCAGCGTTTAAAGCGGCTCAAG GAAATGGCGAAGAACGGTCTAGGAACAAGATTGTTGTTGAAGCAGCAACATGATGATTTTCCAGAGCACTCTAGTAAGAG AACCATGTACAAGGCACAAGATGGGATCTTGAAGTACATGTCGAAGACAATGGAGCGATATAAAGCTCAAGGTTTTGTTT ATGGGATTGTGTTAGAGAATGGGAAAACGGTAGCGGGATCTTCTGATAATCTCCGTGAATGGTGGAAAGACAAAGTGAGG TTTGATAGGAACGGCCCAGCTGCTATAATCAAGCACCAAAGGGATATCAATCTTTCTGATGGAAGTGATTCAGGGTCTGA GGTTGGGGATTCTACCGCACAGAAGTTGCTTGAGCTTCAAGATACTACTCTTGGAGCTCTGTTATCGGCTCTGTTTCCTC ACTGCAACCCTCCTCAGAGGCGGTTTCCGTTGGAGAAAGGCGTGACACCGCCATGGTGGCCAACGGGGAAAGAAGATTGG TGGGATCAACTGTCTTTACCCGTTGATTTTCGAGGTGTTCCGCCACCTTACAAGAAGCCTCATGATCTCAAGAAGCTGTG GAAAATTGGTGTTTTGATTGGTGTAATCAGACATATGGCTTCTGACATTAGCAACATACCCAATCTCGTGAGACGGTCTA GAAGTTTGCAGGAGAAAATGACGTCAAGAGAAGGCGCTTTATGGCTCGCTGCTCTTTACCGAGAAAAGGCTATTGTTGAT CAAATAGCCATGTCTAGAGAAAACAACAACACTTCTAACTTTCTTGTTCCTGCAACCGGTGGAGACCCAGATGTTTTGTT TCCTGAATCTACAGACTATGATGTTGAACTGATTGGTGGCACTCATCGGACCAATCAGCAGTATCCTGAATTTGAAAACA ACTACAACTGTGTTTACAAGAGAAAGTTTGAAGAAGATTTTGGGATGCCAATGCATCCAACACTCCTAACATGTGAGAAC AGTCTCTGTCCTTATAGCCAACCACATATGGGATTTCTTGACAGGAACTTAAGAGAGAATCACCAAATGACTTGTCCTTA TAAAGTCACTTCCTTCTACCAACCAACTAAACCCTATGGTATGACGGGTTTAATGGTTCCTTGTCCGGATTATAACGGGA TGCAGCAGCAGGTTCAGAGCTTTCAAGACCAGTTTAATCATCCCAACGATCTCTACAGACCAAAAGCTCCACAAAGAGGC AACGATGACTTGGTTGAGGATTTGAATCCTTCTCCTTCGACGCTGAATCAGAATCTTGGTTTAGTCTTACCTACTGACTT CAATGGAGGTGAGGAAACAGTAGGAACAGAGAACAATCTGCATAATCAAGGGCAAGAGTTGCCCACATCTTGGATTCAGT AAAGAAAGCTTCAGAGTTTTCTTTTTATGTTTTCTAGTCTTTATAGCTTTGTCTCTTGCTTATTCTCTCATTAAACACAG TTTTTGATCTCTCCATTTCAT ......N....................................................N.....N.....N........ ........................N....................N.....N....................N....... ...........................................i..............................N..... .........N.......N.............................................................. ...i.............................................N..N........................... ....N...............i................N...........i.............................. N.................N.......................................N..................... ..........................................................N..................... ................................................................................ ....................................................N........................... .............................................................N.................. ..................................i............................................. .................i......................N....................................... .........i............................................................N......... .................N..N........................................................... ............................................i.....N...................N......... ...........................i......................................i............. ...................................N....i...........i..........................N ................................................................................ ....N........................................................................... ..N............................................................................. ..........................N..................................................... ..................... Pos Score Pred ------------------------ 7 0.143 - 60 0.415 - 66 0.302 - 72 0.239 - 105 0.322 - 126 0.486 - 132 0.255 - 153 0.474 - 204 0.664 Yes 235 0.395 - 250 0.277 - 258 0.336 - 324 0.641 Yes 370 0.319 - 373 0.177 - 405 0.412 - 421 0.537 Yes 438 0.306 - 450 0.638 Yes 481 0.252 - 499 0.305 - 539 0.337 - 619 0.115 - 773 0.474 - 862 0.174 - 915 0.651 Yes 978 0.807 Yes 1001 0.216 - 1050 0.796 Yes 1111 0.275 - 1138 0.247 - 1141 0.164 - 1245 0.689 Yes 1251 0.255 - 1271 0.297 - 1308 0.556 Yes 1347 0.810 Yes 1396 0.344 - 1401 0.777 Yes 1413 0.807 Yes 1440 0.473 - 1525 0.416 - 1603 0.206 - 1707 0.051 -

Explain the output -- Processing locus 238: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCAGTGTTCATATTAGAGTAAGTTAGTTATATCTGCTAAAGTTAGATTCAGATCAGTGTTCTGTAGTAAAATTGATGC AAGTTATTGTCAACAACCTGAGACATTTTTTATACTTATATGAGATATGTTTCTGCTTGCAGCACAAGTGAGCTAGCTTT GAGAAAGATTCAATTTTTGAGCCTTAAGATTGTGATGGGAGTTGTGTCTATTTCAAGTTCAGCTGCAAGATCTCCACTCG GGTTGGGCAACGATCTTTTGACACATCGGTCTTCACTTAAAAAGCCATCCATTGTTGCTTTTAAAGCCGATGATTCCAGC AATTCAGCTTTGATCATTCCTCCTCGTGAGCAAGTTTTGATTCCTGCTGAAAAGCATAATGAGAAGAAGAGAGAAGTAAC GAGGAGAAAGCCTTGTAAAACCCCGAAAAAACCGCTTTCCTTGGAACATAATTCGGCTCCTTCGTGTTCGTTAGGAGTAG ATTACAATGAAGCTGCTGCAAGACTTGAAAGCATATACAAGCTTAGTCCTGCAACAACCACATTAGTCGAGGATGATGTT GAAGATGGCGGCTCTAAAGCCAAAGTTTCACGGAGGAGAAAAAGGAAAGAAAGTGGTGAGGAGAAGAAAGTGGTTGTGAG GAATAATGTGAAGAAGGAAAAACGTATGAGTCTTGATAAACGGATTGCTTTGAAGAGAAACGTTCAAGAGAAACCAGTCG TTGCTTCTGTTGATAAGAAAGTAACAAAGAGGCAGCAAGAAGAGGAGAAGATCGAGAGGCTTGTGCGGGACTATTCAGCT TCTAATGATATAGTGAGCTTGGACTGGAAAAAAATGAAGATACCTCCTGTTCTTTCTTCTACTGAACATGCTTGGTTGTT CAAGTTGATGCAACCTATGAAGGCTCTTCTTGAAGTGAAAGATGTATTGCAAAAGAGCTTGGGAAGAGAGCCTAGGGAAG CTGAAATAGCCGGGGAGATCAATATGACTGCGGGTGAAGTAAAAAAGAAAATCGAAATAGGTAGAGCTGCAAGAAACAAA CTTATTAAGCACAATCTCCGGCTTGTCTTGTTTGTTATGAACAAATACTTTCATGAGTTTACCAACGGACCAAAATTCCA AGACCTTTGTCAAGCGGGTATGAGAGGGCTTATCACAGCAATTGACCGCTTTGAGCCGAAAAGGAAGTTTCGTCTTTCGA CTTATGGTTTGTTTTGGATTAGACATGCCATCATACGGTCAATGACAACTTCAAACTTCACTCGTGTCCCATTTGGACTT GAATCGGTTAGAGTGGAGATATATAAAGCAAAGACTGAGCTATGGTTTGAGATGGGAAGACCTCCAACGGAGGACGAGGT AGTTGAGAGACTCAAGATAACACCTGAGAGATACCGTGAAGTATTGAGGGCTGCAAAACCGGTTTTGTCACTAAATTCAA AACATTCGGTTACTCAAGAAGAATTCATCAACGGAATCACAGATGTTGATGGTGTTGGAGCTAATAACAGCAGACAGCCT GCTCTTCTCAGGCTCGCTCTCGACGATGTGCTCGATTCATTGAAGCCGAAAGAGAGTCTGGTTATTCGACAGAGGTACGG TCTTGATGGAAAAGGCGACAGGACTCTAGGAGAGATAGCTGGAAATCTCAACATCTCTAGAGAAATGGTTAGGAAACATG AAGTCAAGGCCTTGATGAAGCTTAAGCATCAGGCCAGAGTTGATTACCTTCGTCAATACATCGTCTAAAACCAAACCGAA CCGAACTGAGCCGCCTCACTCAACCATTTGTGCATACTTGGTATGATTTCCCGACTTTACGAGATACTTCACACCCTCTT ATAACCG ............................................................................N... .......................................N......N................................. ..................................N............................................. .....................................................................N.......... ..........................................................N..................... ................................................................................ ......N.................................................................N..N.... ....N........................................................................... .....N...................i...................................................... ................................................................................ ....N............................N.................................N............ .......N........i........................N...................................... .......................i........................................................ ....................................i...............N........................... ...................i............................................................ ...N....................N................N...................................... .........................................i.........i............................ ................................................................................ ..........................................N.....N............................... .........................N...................................................... .....N..........................................................i............N.. ..............N................................................................. ..........................................N..................................... ....... Pos Score Pred ------------------------ 77 0.266 - 120 0.308 - 127 0.146 - 195 0.197 - 310 0.215 - 379 0.159 - 487 0.306 - 553 0.223 - 556 0.425 - 565 0.439 - 646 0.169 - 666 0.627 Yes 805 0.111 - 834 0.386 - 868 0.456 - 888 0.411 - 897 0.717 Yes 922 0.449 - 984 0.583 Yes 1077 0.507 Yes 1093 0.197 - 1140 0.603 Yes 1204 0.226 - 1225 0.232 - 1242 0.471 - 1322 0.670 Yes 1332 0.578 Yes 1483 0.204 - 1489 0.362 - 1546 0.352 - 1606 0.188 - 1665 0.768 Yes 1678 0.455 - 1695 0.217 - 1803 0.053 -

Explain the output -- Processing locus 239: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTAACAGTGGCTGCTATATAAATATATTTATACTATCCTAAATCATTATCTTAAAAATTAAAATGCTCTAATAATGTTT TTTGCAATTTTTATATAGTAGTCATTAAAAAAAAAAAAAAGTGTAAACAAGAGAATCACGCAAAGGAAGCTTTTGATACA GAAGGAAAAGGGACCCACAATGAAGACAATACTGCCCACAGTGAGAGCCACACAAGCTCTCTTCTTCCTCCACCGTGTCC TCCTCTCTCCCTCTTCTTCTTCTTCAATTCAGGCAATCATTCTTCATTTTCCATTTTGCTTCTTCTTCCTTCTTCTCCTC TCTCTGACACCAACAATGGCGAGACTTACCTTGAGACCCCACAATCACTTCTTCTCATCTCCTATCTACGCACATAAACA ACCTTTCCTCTCCGTATACACCATCTTCCCCCACCACCACCAAAATCCTCTTATCAAATCAAGGGTTAAGTGCTCCGCCA GTGGTACTGAACGAGTCAGAGAGAGTAAGAAGCTTCCTCCAAAGGATCCTATCGAAGACCCTAAACCCCAGCTTCCGATT CCTGAAGTTCTCAGTACGGAAACGGGTTTTGAACAGAATTGGCCTCCATGGAAAAATATACCTCAGAGATATAAGCTTAT CGGCGCTACTTCTCTCGCCTTTGTTATCTGCAATATGGACAAGGTGAACTTGAGTATTGCTATAATACCAATGTCACACC AGTTTGGATGGAGCTCATCTGTGGCTGGGTTGGTCCAGTCTTCCTTCTTTTGGGGTTATGCCTTGAGCCAGTTGCCTGGA GGTTGGCTTTCCAAGATTTTTGGCGGAAGAAAAGTGCTTGAGATTGGTGTCTTTACATGGTCTTTCGCTACAGCTCTTGT TCCACTACTTGCTGGATTTATGCCCGGTTTGATCTTTTCTCGAATTTTGGTGGGAATAGGAGAAGGTGTTTCCCCATCGG CTGCGACAGACCTTATTGCCAGGACAATACCTGTTAAGGAGCGCTCAAGAGCGGTAGGCTTTGTTTTTGGAGGACTGAGT TTGGGGAGTGTAATGGGGCTTCTCTTGGCTCCTCCCATCATCGAAACCTTTAATTGGGAATCTGTCTTTTACTTGTTTGG TCTCCTTGGTGTTGGCTGGTTCGTCGGGTTTCAGTTTCTTAATGAAGAAGAAGTTTCATATAAAGGTAATGAGATCTCTA CTTCGCATAAATCAGAAAACGCTACAAAGGAAGAGTTGGGGAGCTCATTGAAGGAGATTCCATGGAAGTCATTTTTCCAA AGCCCGGCTGTTTGGGCAATGATATACACTCACTTTTGTGGAAGTTGGGGTCACTATACCTGTTTATCTTGGCTACCAAC TTATTTCAGTGAGGCTCTGAGTCTAAATTTGACAGAAGCTGCTTGGGTTTCCATCCTTCCTCCACTGGCTTCGATTGTCG TGACAAGTCTTGCTTCACAATTTGCTGATTACTTAATTACAAATGGAGTTGATACTACCACGGTCCGAAAGATTTGTCAA ACGATTGCGTTTGTGGCACCAGCGATCTGCATGACACTTTCATCTGTGGACATTGGATTGCCACCATGGGAAATTGTAGG AATTTTGACAGCAGGCTTGGCCCTATCAAGTTTTGCTTTATCAGGACTTTATTGTACTCATCAGGACATTTCACCCGAAT ATGCAAGCATACTCCTGGGTATTACAAACACAGTCGGGGCAGTACCTGGAATTGTAGGAGTGGCCCTAACTGGTTTTCTC CTAGACTCGACCCATTCCTGGACTATGTCTCTATTCGTGCCATCAATATTCTTCTACTTGACGGGGACGGTTGTATGGTT GGCATTTGCAAGCAGTGAGCCTCAGACGTTCAGAAAGGAAGACTCATAAGTTTTGTTATTGAGGCACAAAAACATGGAGA AACTTTTGTTATTGTATAGGTAAAAAAGAAATGGTAAAAAATTTTCTGTTGTGAAAGCAAAAACAAAG ...............................................................i..........i..... ................................................................................ ...................N............................................................ ................................................................................ ...............i................................................................ ................................................................................ ................................................................................ ...............................................N................................ ..................................N...................................N......... .......i.................................................N...................... ........................................................i....................... ...................i............................................................ ................................................................................ ............i................................................................... .........................................N..........................N........... .............................................................N.................. ..................N............................................................. ................................................................................ ..........................................N..................................... ..............................N..................................N.............. ................................................................................ N............................................................................... ........................N.................................................i..... .........................................................................N...... ..............................N..................................... Pos Score Pred ------------------------ 64 0.582 Yes 75 0.705 Yes 180 0.298 - 336 0.866 Yes 608 0.481 - 675 0.437 - 711 0.262 - 728 0.575 Yes 778 0.129 - 857 0.674 Yes 900 0.577 Yes 1053 0.505 Yes 1162 0.241 - 1189 0.302 - 1262 0.289 - 1299 0.227 - 1483 0.377 - 1551 0.188 - 1586 0.476 - 1681 0.300 - 1785 0.304 - 1835 0.543 Yes 1914 0.247 - 1951 0.085 -

Explain the output -- Processing locus 240: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCATAGCGTTTTTTACTGAAGACAAGTACCTCTCACAAATAGTAGGTAAACCAAATTTGAATTACAGAAATAGTCAAA GACATTAACTAGCGTCTATAACTAATTAAAATCTTAAACCAAATCCCCAAACACAGAGTTGGGGGTTTCTCAAAGGATAA GAAAATGACTTTTCTTCAATTATATATAGGGCCACTAAACCCAACAAATATACTTGATCCAGTTATAAAACCTTTCCTAC GAAAACTCAAAGAAACAATGGCCATCAAATCCATATTTATCATCATCATCATCAGCATCATCACATCAATATCTGAACTT TATGCTTTGGATCCTTCGTTTCTTCGTCTTTCGACCTCTCTCCAAAGAAGTAGCTTTCCTCAAGATTTTCGGTTTGGTGC AGCTTCGAGTGCTTATCAGAGTGAAGGAGCAGCCAACGTTGATGGAAGAGAACCAAGCATATGGGATACGTTTACTAAAC AATATCCAGAAAAGATTTCAGATGGTAGCAATGGCGACGTTGCAGACGAATTTTATTACCGTTTCAAGGAGGACGTGGCA CATATGAAAGAAATAGGGCTGGACTCATTTAGGTTTTCGATTTCTTGGTCCAGAATACTACCTCGAGGTACGGTTGCTGG AGGAGTAAATCAGGCAGGAATTAACTTCTACAATCATCTCATCAATGAACTCATTTCAAATGGTATAAGGCCTCTGGTTA CTCTGTTTCACTGGGATACACCTCAGGCACTTGAAGATGAATATGGCGGGTTCTTAAACCCACAAATCGTGAAAGATTTC GTAGAATATGTGGATATATGTTTCAAAGAGTTTGGAGATAGAGTGAAGGAGTGGATCACAATCAACGAGCCTAACATGTT CGCAGTGTTAGGTTACAATGTCGGTAATATTGCTCCGGGGCGTTGTTCGAGCTATGTTCAAAACTGCACGGTTGGGAACT CAGCCACTGAGCCATATCTCGTGGCTCATTACTTAATCCTTTCTCATGCCGCTACAGTCCAACTCTATAGAGAGAAATAT CAGAGTTTTCATGGAGGGACCATAGGAATGACTATTCAAACATATTGGATGATTCCAAAATATAACACTCCTGCTTGTAG AGAAGCTGCCAAAAGGGCTCTTGACTTCTTCTTCGGATGGTTTGCTGATCCGATCACTTACGGAGATTATCCAAAAACAA TGAGAGAGTTGGTGGGAAATAGACTCCCAAAATTCACAAAAAAGCAGTCAAAAATGGTGAGAGGATCCTTTGACTTTTTT GGACTCAACTATTACACTTCAAGATACGTTGAAGATGTTATGTTCTACGCTAACACCAATCTTAGTTACACAACAGATAG TCGCGTAAATCAAACAACGGAGAAAAATGGAGTTCCTGTGGGTGAACCGACGAGTGCTGATTGGCTCTTTATATGTCCAG AAGGGTTTCAAGATGTTCTATTATACATAAAATCGAAGTTTCAAAATCCAGTAATCCTCGTCACTGAGAATGGAATGCCT AGCGAGAATGACAAATCATTGTCCGTGAACATAGCACTCAATGACGAGGCAAAGATCAAATATCACCAACTCCATCTCAC TGCTCTTCTAGAAGCAGTCAGTCAAGGAGCAGATGTGAGAGGATATTATATATGGTCATTAATGGATGATTTTGAGTGGG AATTTGGTTACAAATATAGATACGGATTGGTCTACGTTGATTTTCAAGATGGACTTAAACGACACCTCAAGAGTTCTGCT CTTTGGTACCATCATTTTCTATCCAACTCATCCTCCTATCAAATGGACTAGCAAGGAATATCTAAAATTAATATCTTCAC TTTATACAACTTTCTTATGTATTCCTAAGAATAAAATGAAACATGCAACTTTAGCAGTACTGTTTATCCC ................................................................................ ................................................................................ ....i........................................................................... .................i.............................................................. .........................................N..................N................... .....................N........N................................................. ...N............................................................................ ............................................N..............i.................... ....................................N.....i..................................... .......N.........N.........................................................i.... .................N...................................N.......................... .............................................N.................................. ..........N................i....................i............................... ....................................N..........................................N .....................................................i.......................... ..................................N....i........................................ ..........................i.............................................N....... ............N........................................................N....i..... .......i................................N....................................... ................................N..................N.........N...N.............. ................................................N............................... ..........................................N..................................... ................N..................N......N........................... Pos Score Pred ------------------------ 165 0.675 Yes 258 0.629 Yes 322 0.420 - 442 0.189 - 461 0.246 - 502 0.211 - 511 0.496 - 564 0.456 - 685 0.183 - 700 0.587 Yes 757 0.426 - 763 0.614 Yes 808 0.180 - 818 0.296 - 876 0.504 Yes 898 0.333 - 934 0.147 - 1006 0.278 - 1051 0.336 - 1068 0.702 Yes 1089 0.771 Yes 1157 0.410 - 1200 0.331 - 1254 0.751 Yes 1315 0.220 - 1320 0.633 Yes 1387 0.504 Yes 1433 0.438 - 1453 0.133 - 1510 0.289 - 1515 0.796 Yes 1528 0.522 Yes 1561 0.297 - 1633 0.313 - 1652 0.168 - 1662 0.499 - 1666 0.121 - 1729 0.393 - 1803 0.469 - 1857 0.069 - 1876 0.068 - 1883 0.057 -

Explain the output -- Processing locus 241: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTGATCCGTTTGATTTCTCGACGAAATCTGATCCATACAAATTACTTATCATTCAATTTTCGTATTGATTCTGTGTTTT ATTTTCGCAGTTATGTTATAGCCTAAGTTAAGAATCTTTATCATCTTCATGAGACTCGAATTGTTGGAAAATGTTATAAT TTCACAAGTAATCTTTGATTTCATGATTAACCAATCAATCAATCGTAACACAATGATTCCAATTTCACTAAACTTCAATT TTACAGGTTTGTTCTGTATTTTGGTTAGAGTGTTAAAAAACTATTGGAAGATGGGTTCTGGTCAATGGCATATGGAGAAG AGGTCTACTTTAAAGAATGATTCGTTTGTAAAAGAATACAATCCAGTCACGGAAACTGGGAGTCTCTCTATCATTGTTCT TGGTGCTTCTGGTGATCTTGCTAAGAAGAAGACTTTTCCTGCACTTTTCAATCTATTTCATCAGGGATTTCTTAATCCAG ATGAAGTTCATATATTTGGATATGCAAGGAGTAAGATTACTGATGAGGAGCTTAGAGATAAAATCCGCGGATATCTTGTT GATGAGAAGAATGCATCTAAGAAAACAGAAGCTTTGTCCAAGTTTCTAAAGCTGATTAAGTATGTGAGTGGACCTTATGA TTCTGAGGAAGGGTTTAAGAGATTAGACAAGGCGATTTTGGAGCACGAGATATCTAAAAAGACTGCTGAAGGATCTTCGA GGAGATTGTTTTATCTTGCACTTCCTCCGTCTGTATACCCTCCTGTAAGCAAGATGATCAAGGCATGGTGCACTAACAAA TCTGATCTTGGTGGATGGACTAGGATTGTTGTTGAGAAACCATTTGGAAAGGATTTGGAATCTGCAGAGCAGCTGAGTTC TCAGATTGGAGCCTTGTTTGAAGAACCACAGATTTATCGTATTGATCACTATCTGGGAAAAGAGTTAGTTCAAAACATGT TGGTTCTTCGGTTTGCCAATCGCTTGTTTTTGCCACTATGGAACCGCGACAATATTGCAAACGTACAGATCGTTTTCAGA GAAGATTTTGGAACTGAAGGTCGCGGTGGATATTTTGATGAATACGGAATCATTCGTGATATTATTCAAAACCACTTGCT CCAGGTTCTTTGCCTTGTCGCCATGGAGAAACCAATCTCTCTTAAACCCGAGCACATCCGGGATGAGAAAGTGAAGGTTC TTCAATCTGTGATTCCCATAAAAGATGAAGAGGTGGTTCTTGGACAATATGAAGGATATAGAGATGATCCAACTGTTCCA AATGACTCAAACACTCCAACGTTTGCCACAACAATTCTTCGCATTAACAATGAAAGATGGGAAGGTGTTCCTTTTATACT GAAGGCCGGAAAGGCAATGAGTTCAAAGAAGGCAGATATTCGCATTCAGTTTAAAGATGTTCCTGGCGACATATTCAAAT GTCAAAACCAAGGGAGGAACGAGTTTGTTATACGCTTGCAGCCTTCAGAGGCCATGTACATGAAGCTAACTGTGAAGCAA CCGGGTCTGGAGATGCAAACCGTGCAGAGTGAACTAGACCTATCGTACAAGCAGCGTTACCAAGATGTCTCAATTCCAGA GGCTTACGAACGCCTTATTCTTGACACAATCAGAGGCGACCAACAACACTTTGTTCGCAGAGACGAATTAAAGGCAGCTT GGGAAATCTTCACTCCACTGCTTCACAGGATCGACAAGGGAGAAGTGAAATCGGTCCCATACAAACAAGGTAGCCGTGGC CCAGCGGAAGCAGATCAGCTACTTAAGAAAGCTGGTTATATGCAGACTCATGGCTACATTTGGATCCCTCCTACATTGTA AATGGTATAATCATAGAGATGATGATGATGAGTACACACAAAATAAGAGGGTTTTTTTTCTTTTTTTTCCTTGCTTGCTG AATAAAAACTCTTCACCAGT ................................................................................ ............N...................................N.....................N......... ......................N.............................N........................... ..................................................i.............N......N........ ................N............................................................... ................................................................................ N....................N....................N..................................... ................................................................................ .....................................................i..........i............... ..............N................................................................. ............................................................................i... .....................................N.......................................... .....................................N.......................................... ......................i.......................................N................. ........................N.......................N..............N................ ................N.......................................N.....................i. .....................................................N.....N.................... ............i...................................................N............... ................................................................................ ................................................................................ .......................................N.........N.............................. .................... Pos Score Pred ------------------------ 93 0.201 - 129 0.164 - 151 0.142 - 183 0.151 - 213 0.296 - 291 0.675 Yes 305 0.296 - 312 0.441 - 337 0.112 - 481 0.151 - 502 0.258 - 523 0.235 - 562 0.076 - 571 0.260 - 622 0.169 - 637 0.203 - 774 0.725 Yes 785 0.601 Yes 815 0.483 - 957 0.734 Yes 998 0.178 - 1078 0.131 - 1143 0.540 Yes 1183 0.120 - 1225 0.276 - 1249 0.220 - 1264 0.176 - 1282 0.130 - 1330 0.443 - 1337 0.647 Yes 1377 0.322 - 1417 0.219 - 1439 0.647 Yes 1494 0.304 - 1500 0.424 - 1533 0.520 Yes 1585 0.497 - 1800 0.109 - 1810 0.367 - 1842 0.306 - 1859 0.074 - 1862 0.052 - 1865 0.110 - 1868 0.067 -

Explain the output -- Processing locus 242: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 243: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 244: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGGCGGTGACCATAGTTATCGGCTCACCTTTCCGATGCTTCTGTCTCAATTGCGTCAAAGTCACTCTCTGATTCGAGTTC TGCGGTTTCGGTCCTCCGTAAACCGTGTCCTCCGGTACGTTGCTCATAAACCGGACGGTGGTGATGGCTCTCGAAGCACG AGAGCAACAACTCCTGACGAGCGAAGCAGCCATTGTTGTTGCTGATGAAAGCGATTTTGGAATTAGTTATAAATTTAAAG ACTTTTGGATAAATATTTTTCTTTTAAGAATAAAAACGATGTTGGCGACTCACGCGCTGGTGACTCCGATTCCAAAGTTG ACTGCTTTCGTACTCTCTCTATCTACTACTCCATCAAGATTGTCTGGTTCTGTTCCGGGTTTGATTGATTTCTACCGTCG CCGGAGATTTTGTCGTGTAATCACCTCCATGGCTGAAGCGGGATCGGTTTACGTCGATGTAGCTGGGGAATCGAAGTCGG AGCATGTTACTGGGAAGTGGTTTTCGGTGCCGGAGCTCCGACTGCGTGACCACCGGTTCATAGTTCCTCTTGATTATTCC AAAAGTTCTCCCAAAATTACCGTCTTTGCTCGAGAGATCGTTGCGGTTGGGAAAGAGGAGCAAGCAATGCCGTATTTATT GTATTTGCAAGGTGGTCCAGGATTTGAAGGTCCAAGGCCTAGTGAAGCTAGTGGATGGATTCAAAGAGCTTGTGAAGAGT TTCGAGTGGTCTTACTCGATCAACGAGGAACAGGCTTGTCCACTCCTTTAACATGTTCCTCTATGCTTCAATTCAAATCA GCAAAGGAATTAGCTGATTATTTGGTTCACTTTCGAGCTGATAATATAGTTAAAGATGCTGAGTTTATTAGAGTTCGTCT TGTTCCTAAGGCTGATCCTTGGACAATTTTGGGTCAGAGTTTCGGTGGCTTTTGTGCACTTACATATCTGAGTTTTGCAC CCGAAGGACTCAAACAAGTGCTAATAACTGGAGGAATTCCACCAATTGGTAAAGCATGTACAGCAGATGATGTATATGAA GCCGGTTTTGAACAAGTTGCTCGCCAAAATGAAAAATACTACAAGAGATTCCCTCAAGACATTGAGATTGTTCGTGAACT CGTAAATTACTTGGCTGAATCTGAAGGTGGCGGGGTACCTCTCCCATCAGGAGGCATTCTTACTCCTAAGGGTCTTCAAA CTCTTGGTCTTTCTGGTTTAGGATCAAGTACTGGTTTTGAGCGCCTGCATTATATGTTGGAGAGAGTATGGGATCCTATT TTAGTTACTGGAGCTCCAAAGTGTATTAGTCAGTTCTTCTTAAACGCTTTTGAGAGTTGGCATTCTTTTGATACAAATCC ACTATATGCTCTTCTCCACGAGGCCATATACTGTGAGGGTGCTTCATCAGGATGGTCTGCTCATAGATTACGGGATAAAT ATGAGTACAAATTCGACGCAATGAAGGCAGTCAAAGAAAGTCAGCCTGTACTCTTCACAGGAGAGATGATATTTCCATGG ATGTTTGATGAAATTCATGCCTTGAAGCCATTTAAAGCTGCCGCCGATCTTTTGGCCAAGAAAGAGGATTGGCCTCCATT ATATGATGTTCCTAGATTGCAGAATAACAAGGTGCCTGTTGCTGCAGCGGTTTACTACGAAGATATGTATGTAAACTTCA AGCTGGTGACGGAAACAGCTTCTCATATATCGGGTATCAGACTTTGGGTAACGAATGAGTTTATGCATTCGGGTCTTCGT GATGCTGGAAGACAAATTATTGATCATTTGTTGGGAATGATCAATGGGAAAAAGCCTCTCTTTTGATTCCTTGTACTAGT CAGTACTTTGTGACTTGTGGTTTTCTTCGTTATACACTTTCTTTGAGAATTAAATGAGTAAAAAAGAAAGAGAATGAATA AAAGA ..................................N............................................. ...............................................................i................ ............................................N................................... ......................................i......................................... ................................................................................ ............................i...........................N....................... ...N............................................................................ ..................................................................i............. ......................................................N......................... ....................................................N.........N................. .......................................................N........................ ................................................................................ .......................................................N..........N..i.....N.... ............................N................................................... ................................................................................ .....................................................i.............N............ ................................................................................ .....N.............................................N............................ N...................i............................................i..........N... i......N........N............................................................... ..N..N..........................................................N...N........... ......................................................N.......N................. .....................................................N...................N...... ..... Pos Score Pred ------------------------ 35 0.414 - 144 0.670 Yes 205 0.149 - 279 0.745 Yes 429 0.692 Yes 457 0.157 - 484 0.242 - 627 0.587 Yes 695 0.138 - 773 0.103 - 783 0.186 - 856 0.382 - 1016 0.302 - 1027 0.249 - 1030 0.515 Yes 1036 0.188 - 1069 0.240 - 1254 0.599 Yes 1268 0.261 - 1366 0.291 - 1412 0.460 - 1441 0.288 - 1461 0.720 Yes 1506 0.630 Yes 1517 0.325 - 1521 0.632 Yes 1528 0.124 - 1537 0.429 - 1603 0.185 - 1606 0.136 - 1665 0.383 - 1669 0.126 - 1735 0.294 - 1743 0.275 - 1762 0.205 - 1797 0.229 - 1804 0.291 - 1894 0.034 - 1914 0.055 -

Explain the output -- Processing locus 245: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGTGGTGGTAAATGACAAAGACACGTCACAAAGTTGAAGACGTTCGTCCCTTGCCCGCCAGTCAAGTTATGACTTATGA GAGTCACATAACCAATATTAATATATCTCCTATTTTGTCTACTTTTTTTTACAGAAGTCCAAATTTGGTTCTTGCGCCTT CGTGGCACGCAAAATATCGATTACTTCAAAAGAATTATATCAACACAAACACTATAAAGTATATAAGACATCATCGGTGA TGTCATCACCACATCCATATCTTTTCCAAAGTTTTCAACCTTTTATTCCCCGAAATTTTTTAGCAAATTCTCTACCTCTA TCTCTCTCTTCTCTGGGAGGAAAAATGGAGATATCGGTTGCATCGGTAACAATATCAGTAGTTCTAGCTGTTGTGTCATG GTGGATATGGAGAACTTTACAGTGGGTTTGGTTTAAGCCAAAGATGCTTGAGCATTACCTGAGAAGACAAGGTCTTGCCG GAACACCTTACACGCCTCTAGTCGGCGATTTGAAGAAGAATTTCACCATGTTGTCGGAGGCAAGGTCCAAACCCCTCAAA CTAACGGATGACATCTCACCTCGTGTCGTGCCTTATCCCTTGCAAATGTTCAAGACTTACGGAAGGACTTACTTTACATG GTTTGGACCTATCCCAACCATCACAATAATGGATCCTGAGCAAATCAAGGAAGTGTTCAACAAAGTTTATGATTTCCAAA AGCCGCATACATTTCCTTTGGCCACAATTATAGCTAAAGGACTCGCTAATTATGATGGTGATAAATGGGCAAAACACCGA AGAATCATCAACCCGGCTTTTCATATAGAGAAGATCAAGAATATGGTACCTGCGTTCCACCAGAGCTGTAGGGAAGTTGT TGGCGAATGGGATCAGTTAGTATCGGATAAAGGGTCGTCATGTGAGGTAGACGTTTGGCCTGGGCTTGTGAGTATGACTG CAGATGTGATCTCTCGTACTGCATTTGGCAGCAGCTACAAAGAAGGGCAGAGGATATTTGAGCTCCAAGCAGAACTAGCA CAGCTCATCATTCAAGCTTTTAGGAAAGCTTTCATCCCTGGATATAGTTATCTCCCAACAAAAAGTAACAGAAGAATGAA AGCAGCAGCCAGAGAAATCCAAGTTATACTAAGAGGGATCGTAAACAAAAGGTTAAGGGCCAGAGAAGCTGGTGAAGCAC CAAGCGACGATTTGCTGGGAATACTTCTTGAATCGAATTTGAGGCAAACGGAAGGAAACGGAATGAGTACCGAGGATCTG ATGGAGGAGTGCAAGTTGTTCTATTTCGCCGGGCAAGAGACAACATCAGTACTTCTGGTTTGGACAATGGTGCTGTTAAG CCAACACCAAGATTGGCAGGCTCGTGCACGAGAAGAAGTGAAGCAAGTGTTTGGCGATAAAGAACCTGATGCAGAAGGAC TCAACCAGCTCAAAGTTATGACGATGATATTATATGAGGTCCTTAGGCTATATCCTCCAGTAACCCAGCTGACTCGAGCC ATTCACAAAGAGCTGAAGCTAGGCGATCTGACCCTACCAGGCGGTGTTCAGATCAGTCTACCTATTCTGCTAGTCCAACA CGACATCGAGCTGTGGGGAAACGACGCAGCAGAGTTCAATCCTGACAGATTTAAAGATGGTCTTTCAAAGGCAACAAAGA GCCAAGTCTCCTTCTTTCCCTTTGCGTGGGGACCGAGGATATGCATAGGCCAGAACTTTGCCCTGTTAGAGGCAAAAATG GCAATGGCATTGATTCTGCGGAGGTTCTCGTTTGAGATTTCTCCTTCCTATGTTCACGCACCATACACAGTCATCACCAT TCACCCACAGTTCGGTGCTCAGCTTATCATGCACAAGCTCTAATCAGACAACACATTTGTGTTGTTGTCAAATATACAAT AAACTTGTTATACAGAGATGAATCGAGATAGATCATAATACAAAACTCAAATCTTTCCTTTT ............i........................................................i......N... ................................................................................ ...............................................................................N ................................................................................ ........................i....................................................i.. ......i....................................N.................................... ...............................................i................................ .......N.....................................i...............................i.. ............................i.......................................i........... ...................................................N..N.........i............... ..........................................i..................................... ......N................................N.................................N...... ...N............................................................................ ...........................................................................i.... ................................................................................ ..............................................................N................. N.................................................................i............. ....................................................................N........... .................N.....N.........N.............................................. ................................................................................ ........................................................i....................... ........................................i....................................i.. ...i.............................................N.............................. ............................N................................................... .................N............................................ Pos Score Pred ------------------------ 13 0.545 Yes 70 0.586 Yes 77 0.138 - 240 0.488 - 345 0.714 Yes 398 0.597 Yes 407 0.549 Yes 444 0.385 - 528 0.691 Yes 568 0.464 - 606 0.615 Yes 638 0.557 Yes 669 0.768 Yes 709 0.562 Yes 772 0.193 - 775 0.292 - 785 0.619 Yes 843 0.553 Yes 887 0.478 - 920 0.219 - 954 0.470 - 964 0.230 - 1116 0.655 Yes 1263 0.415 - 1281 0.387 - 1347 0.650 Yes 1429 0.208 - 1458 0.385 - 1464 0.269 - 1474 0.292 - 1657 0.573 Yes 1721 0.774 Yes 1758 0.827 Yes 1764 0.702 Yes 1810 0.168 - 1869 0.452 - 1938 0.090 -

Explain the output -- Processing locus 246: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAATTCAATTATTGTTTTCTTAATTAGAAAACAAGACAGCGAGGTGGAGAACATGGAGGACATCAAATCCAATGCAAAT ATACGTAACGAGTTTACTTGAATCTCCATGCAAGTCCCATGCACTCAAACCCGTGTATATAAACCCCTCCAAGGTCATCG TTTAATCATCACCAAAAGAAGCACATCAATAAAGAAAATACAAACCATGGCAATTCCAAAAGCTCACTACTCTTTAGCTG TTCTTGTCCTTCTCTTTGTCGTCGTTTCGAGTAGCCAAAAGGTTTGCAATCCAGAATGCAAGGCCAAAGAACCCTTCCAC TGCGATAATACTCATGCATTCAACCGTACGGGATTTCCCAGAAATTTCACTTTTGGTGCAGCTACTTCGGCTTATCAAAT TGAAGGTGCAGCACATAGAGCACTTAATGGATGGGACTATTTCACGCATAGATATCCAGAAAAAGTACCGGATCGTAGTT CAGGAGATCTTGCTTGTGATTCGTATGATCTTTATAAGGATGATGTCAAACTACTAAAAAGAATGAATGTTCAAGCTTAC AGACTCTCGATAGCATGGTCAAGGGTCTTACCAAAGGGAAGACTGACTGGGGGAGTGGACGAGAATGGGATAACATACTA CAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAGAACCATATGTTACTATATTTCATTGGGATGTTCCCCAAACTT TAGAAGACGAATATGGAGGTTTCTTAAGCACACGTATAGTGGAGGACTACACAAACTACGCTGAGCTTCTATTCCAAAGA TTCGGAGACAGGGTCAAATTTTGGATCACTTTAAATCAGCCTTTCTCTCTTGCAACCAAAGGCTATGGAGATGGATCGTA TCCACCAGGACGGTGCACCGGCTGTGAACTTGGAGGAGATTCTGGAGTTGAACCTTATACAGTTGCACATAACCAACTTC TAGCTCATGCAAAAACTGTATCTTTATACAGAAAAAGATATCAGAAATTTCAAGGTGGTAAGATAGGAACAACCTTGATC GGGAGATGGTTCGCCCCGCTAAATGAATTTAGCGAACTCGACAAGGCTGCTGCAAAACGAGCATTCGACTTTTTTGTTGG ATGGTTCTTGGATCCATTGGTGTACGGAAAATATCCAACGATAATGAGAGAGATGGTAGGAGATAGATTGCCAGAATTCA CTCCTGAGCAATCAGCTTTAGTTAAAGGATCACTTGATTTTCTAGGGTTGAACTATTATGTTACACAATATGCAACTGAC GCACCTCCTCCGACACAACTTAATGCAATAACGGATGCACGAGTTACTCTTGGATTTTATCGCAATGGAGTTCCTATTGG TGTTGTGGCTCCTAGCTTCGTCTACTATCCTCCAGGATTCCGTCAGATTCTAAACTACATCAAAGACAACTACAAAAATC CACTTACCTACATCACCGAAAACGGAGTTGCTGATCTTGATCTTGGAAACGTAACGCTTGCAACTGCTCTTGCCGATAAT GGACGGATTCAAAACCATTGCAGCCATCTCTCTTGTCTTAAATGCGCTATGAAGGATGGATGCAACGTAGCAGGATATTT TGCATGGTCATTGATGGACAATTACGAATTCGGAAATGGTTACACCCTCCGGTTTGGTATGAATTGGGTCAATTTCACTA ATCCTGCTGATCGAAAAGAAAAAGCTTCTGGAAAATGGTTCTCTAAGTTCCTCGCAAAATAAGTTGAAGCCCTAGATTTA TAAATCCAAACCTCGAATGTAATAACAATTGTACTGTATGTATTTTAAGACCTTATGTGTTTTGTATATGATGTTAATAA .....................................................i..................N....... ...........................N..........N......................................... ..............................................i................................. .......................................................N........................ .............N.................................................................. ..........................N...N................................................. ........................N..............N..N...................i...N............. ..............N.................................................N............... .............................i..............N....................N.............. ............N................................................................... ................................................................i.....i......... ................................................................................ ......N......................................................................... .....N................N......................................................... N..........................................N........i........................... .........................................................N...........N.......... ......................N...........N.............................N............... ................................................................................ ..............................................................................N. .........................................N......N......N...N.................... ...N.........i.....................i......................N..................... ..................................N............................................. ................N....................N................N............N..N......... Pos Score Pred ------------------------ 54 0.736 Yes 73 0.323 - 108 0.229 - 119 0.481 - 207 0.854 Yes 296 0.383 - 334 0.347 - 427 0.427 - 431 0.118 - 505 0.131 - 520 0.402 - 523 0.315 - 543 0.615 Yes 547 0.446 - 575 0.272 - 625 0.231 - 670 0.703 Yes 685 0.393 - 706 0.409 - 733 0.440 - 865 0.654 Yes 871 0.841 Yes 967 0.121 - 1046 0.331 - 1063 0.216 - 1121 0.410 - 1164 0.214 - 1173 0.543 Yes 1258 0.339 - 1270 0.431 - 1303 0.249 - 1315 0.284 - 1345 0.398 - 1519 0.473 - 1562 0.351 - 1569 0.454 - 1576 0.408 - 1580 0.251 - 1604 0.175 - 1614 0.734 Yes 1636 0.710 Yes 1659 0.283 - 1715 0.362 - 1777 0.042 - 1798 0.059 - 1815 0.048 - 1828 0.031 - 1831 0.047 -

Explain the output -- Processing locus 247: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGAGCAAGATATCAGAAAAACTGAACCTTTAATCTCAAGAATTTACTTTGATTGGAGCTTGTGAAGTCCTTGTGTTATT GTTGCATGATGTTTTCTTTGAGATAACTTGGCAATGTGTGTTTGGTGTTATTGTAGAGGGAGCAAGACGATGAGTAGAAA CCCTTTCCTGGATCCTACTCTTCAAGAAATTCTTCAAGTGATTAAACCCACACGAGCAGATAGGGATACGAGGATCACAG TCATTGATCAGTTGCGTGACGTCTTACAATCCGTAGAATGTTTAAGAGGTGCTACTGTTCAACCATTTGGATCCTTTGTG TCAAATCTCTTCACACGATGGGGAGACTTAGATATCTCTGTTGACTTATTCAGCGGTTCTTCCATCTTGTTCACTGGAAA GAAGCAGAAACAAACTTTACTCGGACATTTACTACGAGCACTGAGAGCGAGTGGTCTATGGTACAAACTGCAATTTGTTA TTCATGCGCGAGTTCCTATTCTGAAAGTAGTGAGCGGCCACCAGAGAATTTCTTGTGACATTTCTATTGATAACCTGGAT GGGCTTTTGAAGAGCAGGTTCTTGTTCTGGATCAGTGAAATAGATGGGAGATTCCGCGACCTGGTTTTGCTAGTGAAGGA ATGGGCGAAAGCTCATAATATCAACGACTCAAAGACCGGGACTTTCAACTCTTACTCTCTCAGTTTGTTAGTCATCTTCC ATTTTCAGACTTGTGTGCCTGCAATCTTGCCGCCTCTAAGAGTGATATATCCGAAGAGTGCGGTTGATGATCTGACAGGT GTACGGAAAACTGCAGAGGAAAGCATAGCGCAAGTTACTGCTGCTAATATTGCGAGATTTAAATCAGAGAGAGCAAAATC AGTCAATCGAAGCTCGCTCTCGGAGCTGTTGGTCTCTTTTTTTGCAAAGTTTTCAGACATAAACGTGAAGGCCCAAGAGT TCGGTGTTTGCCCGTTTACTGGAAGATGGGAAACTATAAGCAGCAACACTACATGGCTGCCAAAGACATACTCACTCTTT GTTGAAGATCCTTTTGAGCAGCCGGTGAACGCTGCAAGGAGTGTGAGCCGTAGAAATTTGGACAGAATAGCTCAAGTATT TCAGATTACATCGCGCCGTCTTGTCTCAGAGTGTAACAGAAACTCCATCATAGGGATTCTGACAGGGCAACACATTCAAG AGTCTTTGTACAGAACAATAAGCCTCCCAAGTCAACATCATGCAAACGGTATGCACAATGTCCGTAATCTGCATGGTCAG GCTAGGCCACAGAACCAGCAAATGCAGCAAAATTGGTCACAGAGCTACAATACCCCGAATCCACCACATTGGCCACCACT AACACAATCCCGACCACAACAGAATTGGACTCAGAACAACCCCAGGAATCTTCAGGGGCAACCACCTGTCCAAGGCCAAA CTTGGCCTGTAATCACACAAACTCAAACGCAGCAGAAGAGCCCATATAAGAGTGGGAACCGACCATTGAAAAATACATCT GCTGGTTCAAGTCAGAATCAGGGCCACATTGGTAAGCCATCTGGTCATATGAATGGTGTGAACTCTGCAAGACCTGCATA TACGAATGGTGTGAACTCCGCAAGGCCTCCATCAAAAATTCCAAGCCAAGGCGGACAGATCTGGAGACCGAGACATGAGC AGTAGAATGAGGTGTGCAGACATGGTTCAGAGATACTCCTTCACATATTTTGGATGATTTTGTATTCTGACCTTGGGGCT TTTTTTCTATGGATCCATTCGTTA ................................................................................ .....N..N........................i...................................i.......... ................................................................................ .....................................N.......................................... .................i.............................................................. .........................................................N...................... ...N..........................................................................N. ...........................................N.................................... N............................................................................... ..................................................................N............. ................................................................................ ................................................................................ .........................i..........................i........................... ................................................................................ ................................................................................ .......................................N..........N......i..............N....... .....................i.......................................................... ................................................................................ ................................................................................ ................................................N...i........................... .....N....................................................................N..... ......N..............N...............................N.......................... ........N............... Pos Score Pred ------------------------ 86 0.165 - 89 0.278 - 114 0.617 Yes 150 0.688 Yes 278 0.248 - 338 0.549 Yes 458 0.327 - 484 0.250 - 559 0.361 - 604 0.348 - 641 0.431 - 787 0.153 - 986 0.666 Yes 1013 0.653 Yes 1240 0.204 - 1251 0.388 - 1258 0.599 Yes 1273 0.455 - 1302 0.564 Yes 1569 0.492 - 1573 0.650 Yes 1606 0.251 - 1675 0.331 - 1687 0.156 - 1702 0.120 - 1734 0.055 - 1769 0.037 -

Explain the output -- Processing locus 248: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATACCTGAGAGATTCGTGCGTCGACGACGTGAAAATCATTATTATTTACTAATAATAATTGTTATTGTCAATCGATGATA ATTAAATTAAAATTAGTTTAAAAGTGGTGGTGGAGGTGTTTGATGGGTGCTAGTCACAGCACCAACGTTAATAATCATCC TCATTCTCGGAATGCTTCTAATCATCCTCTGACCAACAGTAACAGCACCAGCTCTCGTCACTCTGCTTCATCAAGTGATC GCTTATCTGTTAGTAATTTACGGTCTCAGCTCACAACTATTTACAGAAATCAGGAAGAAGAGGAAGAAGAAGAAGAAGAA GAAGAAGAGGAAGAAGAAGGAGGAAAAGAGAAAAGAGCAGAAGAAGAAGCGAAAAGTTTCTCCCTTGTTAGAGATTTTGA TTTGTCTGGTTTGAACTGCATTAGGGTTTCTAGAAGGAATTATATTCTCATGGATCCTCATAAAAAGGTTGCACTGGAGA CTGAGTTCTTTACAGAATACGGTGAAGCTAGCCGATACCAAATCCAAGAAGTTATAGGGAAAGGAAGTTACGGTGTTGTG GCATCTGCAATCGACACACATTCAGGCGAAAAGGTTGCCATTAAGAAAATCAATGATGTGTTTGAGCACGTCTCTGATGC AACGCGGATTCTAAGAGAGATCAAGCTTCTCAGGTTGCTCCGCCATCCTGATATAGTGGAGATAAAGCATGTAATGCTTC CTCCTTCACGCCGTGAGTTCAGAGACATTTATGTTGTTTTCGAGTTGATGGAATCAGATCTTCACCAAGTTATCAAGGCG AACGATGATCTTACACCTGAGCACTACCAGTTTTTCCTCTACCAGCTTCTTCGTGGTCTGAAGTTCATACACACAGCTAA TGTGTTTCATCGTGATTTGAAGCCTAAAAATATTCTAGCCAACTCTGATTGCAAGTTGAAGATTTGCGACTTTGGGCTTG CTCGAGTGTCCTTTAATGATGCACCTTCGGCTATATTCTGGACTGACTATGTTGCTACAAGATGGTACCGTGCGCCAGAA CTTTGTGGCTCTTTCTTCTCCAAATATACTCCTGCCATTGATATATGGAGCATAGGATGCATATTTGCAGAAATGCTCAC TGGGAAGCCATTGTTTCCTGGGAAGAATGTGGTGCACCAATTGGATATTATGACTGATTTGCTTGGTACTCCTCCTCCCG AAGCTATTGCAAGGATTCGTAATGAAAAGGCGAGGAGATATCTCGGCAATATGAGGAGAAAACCGCCAGTTCCATTCACT CACAAATTTCCTCATGTAGACCCATTGGCCCTTCGCTTACTGCACCGTCTTCTTGCATTTGATCCCAAAGACCGTCCCTC AGCTGAAGAGGCACTGGCAGATCCTTACTTCTATGGTCTGGCAAACGTTGATCGGGAACCATCTACTCAACCGATTCCGA AACTCGAGTTTGAGTTTGAAAGAAGGAAGATAACAAAAGAAGATGTGAGAGAATTGATCTACAGAGAGATATTAGAGTAT CATCCTCAGATGCTGCAAGAATATCTTCGAGGTGGAGAACAGACTAGCTTCATGTACCCAAGTGGTGTTGATCGCTTTAA GAGACAATTTGCTCATCTTGAGGAAAATTACGGTAAGGGTGAGAAAGGCTCACCTCTTCAACGCCAACACGCTTCCTTGC CTAGAGAGCGAGTCCCTGCTCCTAAGAAAGAGAATGGATCACATAATCACGATATCGAGAACAGATCCATAGCGTCTTTA GTCACGACCCTCGAAAGCCCTCCAACGTCACAACACGAGGGGTCAGACTACAGGAATGGGACGAGCCAGACGGGTTACAG CGCTCGCAGCTTGCTGAAAAGTGCCAGCATCAGTGCATCTAAATGCATTGGCATGAAACCAAGAAACAAGTCCGAGTACG GGGAATCAAACAATGATACGGTTGATGCTCTGTCTCAAAAGGTCGCGGCTCTCCACACTTGACTTGTTTCAGAGATTGAG GATTGAAACTTGTTGCTAACAGTAGACGGCTGATTCTACAAGTCTTGTACACATCTCTCAGAGAAGACGAAGAAGAAGTC ..........................................................................N..... ..........................................i..................................... ...........N.................................................................... ................................................................................ ................................................................................ .................................................i.............................. ................................................................................ ....................................................N..N....................N... ....................................................................N....N...... ..............................N................N................................ ....N..........................................................................N ................................................................................ ...............N..N.............................N............N.................. ............................................i...........N...............i....... ..........................i......................i.............................. .....................N............................i............................. .............N.................................................................. ................................N............................................... ..........................................N..................................... .........i.........................................N............................ ................................................................................ .................................N.............................................. .......................................................i........................ ..........................................N.........N........................... ............i...........N....................................................... ................................................................................ Pos Score Pred ------------------------ 75 0.316 - 123 0.681 Yes 172 0.147 - 450 0.629 Yes 613 0.110 - 616 0.300 - 637 0.084 - 709 0.146 - 714 0.481 - 751 0.146 - 768 0.249 - 805 0.200 - 880 0.239 - 976 0.330 - 979 0.188 - 1009 0.270 - 1022 0.479 - 1085 0.531 Yes 1097 0.472 - 1113 0.825 Yes 1147 0.535 Yes 1170 0.741 Yes 1222 0.321 - 1251 0.507 Yes 1294 0.269 - 1393 0.242 - 1483 0.422 - 1530 0.533 Yes 1572 0.311 - 1714 0.476 - 1816 0.676 Yes 1883 0.208 - 1893 0.495 - 1933 0.601 Yes 1945 0.239 -

Explain the output -- Processing locus 249: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTTCTGGTAAGTTTTCTTTTTCTAGTTTGATGATATTTGTGGTCACAACTTTCTTTTTTGCTTTTGTGGTTGAAAATG AAAACTTGCTGTTTTGATTAAGTACTTGAGCTTGTAACTATGTTTTTGTTTCAGCAGGTTTCTTCTTTCAGAGAAAGCAA GTCTGATGATAGAAAGTTCTATATATTCACAGCAACAAAGACCCTTCATTTGAGGACTGATTCTATAACTGATAGAGCAG CTTGGTTACAAGCATTAGCATCTACAAAATGTATCTTTCCTCTGCGGTCACTTAATGGGGATTTCTCCTTCATACCACCA AAGGATTTGTCCATATCAACTGAGAGACTAAAGAAACGGTTGCACGAAGAGGGTATGAATGAGAATCTCGTCAAAGAGTG CGAACAGATCATGCTCTCAGAATTTTCTGAAATGCACGGACAAGTTAAACTTTTACACGAAGAACGGACTAATTTGCTTG ATGCATTGAGGCAGCTTGAGGCAGCTAATCTCGAAGTTGGTGCATCAGGGAAGCATGAGTTTTCAAGCCTAGGACGTGGA AAGTATAGTGAATGCAGCACAACTGCTTCATCTGATGGTAAACAAGAGTTTGAGGATGTGTCTGAGGAAGATGAACCTTC CTTCCACGACACAAAGGAGTTCTTTAACGAACCTAACATTGGTTCTGAATCAAATTTAGCTATTTCTGGACACACGGATA TCAAGAGAAGAACAAAACTTCCTGATCCAGCTGAAAAAGAGAAAGGTGTTAGTCTTTGGTCTATGATCAAAGACAACGTT GGAAAGGATCTCACCCGAGTTTGCCTCCCTGTGTATTTCAATGAACCAATATCATCCCTCCAAAAATGTTTTGAAGACTT GGAGTATTCGTATCTTTTGGACCGAGCATATGAACATGGAAAATCTGGGAATGGTCTCTTAAGAGCCTTAAATGTTGCTG CTTTTGCGGTCTCTGGATATGCTTCCACTGAAGGCAGGCATTGTAAGCCCTTCAATCCTTTGCTTGGAGAAACCTATGAA GCCGACTTTCCTGAAAAGGGAATCCGTTTCTTCTCTGAGAAGGTCAGTCACCATCCAACTGTTATCGCCTGCCACTGTGA AGGTAAAGGGTGGAAGTTTTGGGGTGACACTAACCTCAGGTCAAAGTTTTGGGGGAGGTCGATTCAAGTCGAACCTGTTG GAGTTTTGACTCTGGAGTTCGATGATGGAGAAGTATTTCAGTGGAGCAAGGTAACATCAACTATATACAATATCATACTA GGTAAACTCTACTGTGATCATCATGGTGTGATGCAAATCCGTGGGAACCGCCAATATTCTTGTACGCTCAAGTTTAAGGA GCAATCCATTCTTGAGAGAAATCCCCACCAGGTAAACGGTTTTGTAGAAGACGTAGCAGGGCAAAAAGCAGCAACAATAT TTGGTAAATGGGATGATAGCCTTTACTATGTTGCTGGTGATGGAATCAACAAGTCGAAAGTCAGTGATCCTGCATCAAAT GCCTCGTTACTGTGGAAAAGGACCAAGCCACCGCCTAATGTAACTAGATACAACTTAACCTCATTTGCCATTACTCTGAA CGAGCTAACACCTGGGTTACAGGAGATACTTCCTCCCACAGACTCTAGGCTCAGACCAGATCAACGGCATCTTGAGAATG GTGAATATGAGAAGGCGAACTTAGAGAAACAACGGTTAGAAAGAAGGCAAAGAATGTCGCGGCAACTTCAAGAAAGCGGT TGGAGACCGAGATGGTTTGAGAGACAAGGTGAAAGCGACACCTTCAAGTACACGGGTGGTTATTGGGAAGCACGAGGGCA TAGGAACTGGGATGATTGCCCCGACATCTTCGGGGAGTTCACAGAAGAGCTAGCGGACGCCGCTTAGAAAGCTTCTTGAC TTCATTCCAGGTAATATAACTCATCCTACCAGCTCTCGTACCAGCAAAATAATAAATTGCCCCTTGAATGTTACAATAGA AAACCC ...............................N.............................................N.. .......................................i........................................ .....N.......................................................................... ............................N.........................N......................... ......................................................i...N..................... ..........N....................i................................................ N.....................................................N......................... ...........N......................N....................N..............N......... ................................................................................ ..............................................................N................. ........................................N........................N.............. .............................N.....N..............i....................N........ ..................N........................................................N.... ................................................................................ ................................................................................ .....................N..N....................................................... ......................N.......N................................................. ................................................................................ .......N....N..............N...........N......................................N. .....................................N.......................................... .............................................................................i.. ......i..............................................i.......................... ...........N.................................................................... ...........N.................................................................... ...................................................................N............ ...... Pos Score Pred ------------------------ 32 0.284 - 78 0.222 - 120 0.725 Yes 166 0.208 - 269 0.457 - 295 0.348 - 375 0.553 Yes 379 0.260 - 411 0.319 - 432 0.727 Yes 481 0.374 - 535 0.359 - 572 0.177 - 595 0.141 - 616 0.221 - 631 0.322 - 783 0.187 - 841 0.313 - 866 0.376 - 910 0.384 - 916 0.478 - 931 0.587 Yes 952 0.306 - 979 0.161 - 1036 0.298 - 1222 0.125 - 1225 0.282 - 1303 0.470 - 1311 0.489 - 1448 0.326 - 1453 0.309 - 1468 0.253 - 1480 0.307 - 1519 0.347 - 1558 0.352 - 1678 0.725 Yes 1687 0.581 Yes 1734 0.558 Yes 1772 0.096 - 1852 0.482 - 1988 0.041 -

Explain the output -- Processing locus 250: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTTTTTTATAAATCAAAATTAGGGTTGGGATCCGACTTGAGACAATACTAATTAAGGTAAACCTTCTTAAATGAAAAAG CTTTATCGCTCAAAATACTAATCAAGTTTCAATAAAGAAATGAAAGAAAGCTTTATTAATTATGTCGTGTTCTGTACCGA AGTGATATGGTCGGAGCCACAACTTTTATTCTGTTTTTATTCTCCTATATATATAATAGTTCCTCTACAGATCAAGAACA CAAATCAAAACAACATAATTAACTTACCCAAACAAGAAACCCAAGAATCCAAAATGGAAATGCTCAACGCAATAATTCTC ATCCTATTTCCAATCATAGGGTTTGTATTAATCTTCTCATTCCCAACAAAAACCCTAAAAGCCAAAACCGCATCACCCTC TAATCCGACATCGTACCAACTCATCGGCTCTATTCTCTCCTTCAACAAAAACCGCCACCGTCTTCTCCAATGGTACACAG ATCTCCTCCGTCTCTCTCCGTCTCAAACCATCACCGTCGATCTCCTCTTCGGTCGTCGGACTATCATCACTGCAAACCCT GAAAACGTTGAGCACATTCTCAAAACAAATTTTTATAACTTCCCTAAAGGCAAACCTTTCACCGACCTCCTAGGAGATCT ACTCGGCGGCGGAATCTTTAATTCTGACGGCGAGTTATGGAGCTCACAGCGTAAACTCGCGAGCCACGAGTTTACAATGC GTTCTCTTAGGGAATTCACTTTCGAAATCCTCCGTGAAGAAGTCCAAAACCGCCTCATTCCGGTGCTTTCCTCCGCCGTG GACTGTGGAGAGACGGTTGATTTTCAGGAGGTTTTGAAGCGCTTTGCTTTCGATGTTGTATGCAAAGTGTCGTTGGGTTG GGATCCGGATTGTTTGGATTTGACCCGACCCGTTCCAGAACTTGTTAAGGCTTTTGATGTAGCGGCGGAGATCAGCGCTC GCCGCGCCACGGAGCCGGTTTACGCCGTGTGGAAAGTAAAGCGTTTCTTGAACGTAGGGAGCGAGAAGAGGTTGAGAGAA GCCATCAAGACCGTACACTTGTCTGTGTCTGAGATCATCCGGGCCAAAAAGAAAAGCCTCGATATCGGTGGTGACGTGTC GGACAAGCAGGACCTGCTGTCGAGGTTTCTCGCCGCTGGCCACGGGGAAGAAGCCGTGAGGGATAGTGTAATCAGCTTTA TTATGGCGGGAAGGGATACAACGTCGGCAGCGATGACGTGGCTGTTTTGGTTGTTGAGTCAAAATGATGACGTGGAGACG AAGATACTCGACGAATTGAGAAACAAAGGGTCTTTAGGGTTAGGGTTTGAGGATTTGAGAGAGATGAGTTACACAAAAGC TTGTCTGTGTGAAGCAATGAGGCTTTACCCACCAGTGGCTTGGGACTCAAAGCATGCAGCAAACGACGACATTTTGCCAG ATGGAACACCTCTCAAAAAGGGAGACAAAGTCACTTATTTCCCTTACGGTATGGGAAGGATGGAGAAGGTGTGGGGTAAA GATTGGGACGAGTTTAAACCAAACCGGTGGTTTGAGGAAGAACCGAGTTACGGTACAAAACCGGTTTTGAAAAGTGTGAG TTCGTTCAAGTTTCCGGTTTTCCAAGCCGGACCAAGGGTTTGCATAGGTAAAGAAATGGCTTTTACACAAATGAAATACG TGGTTGGTTCAGTTTTGAGTCGGTTTAAGATTATACCGGTTTGCAATAACCGGCCGGTCTTTGTTCCTCTTCTAACCGCT CACATGGCTGGTGGGCTAAAGGTGAAGATCAAGAGGAGAGAGCAATGTGATTCGATGTATATTTAATTAATTGATTAATC AAGTCATGTCCTTGTAGAATATCCAACTAATATGTAAAATGTGTGGTTTATAAATTAAATCAAATAAATATATTATATAT GTGTG .......................................................................N........ .......................................N.....................i.................. ......i......................................................................... .....................................................i.....i.................... ................................................................................ .....................................................................i.......... ................................................................................ ................................................................................ ....................................N.......................................i... ................................................................................ ....................................................N......N.................... ........................................................N....................... ................................................................................ ................................................................................ ................................................................................ ..i.............................N..............................N..N............. ...............................................................N................ ................i....................................N.......................... N.................................................i........i.................... ................................................................................ .......................................................N..............i......... ................................................................................ ...N........................................i.........N......................... .....N.........................N......N.......................................N. ..... Pos Score Pred ------------------------ 72 0.391 - 120 0.421 - 142 0.529 Yes 167 0.551 Yes 294 0.724 Yes 300 0.594 Yes 470 0.671 Yes 677 0.387 - 717 0.751 Yes 853 0.246 - 860 0.120 - 937 0.252 - 1203 0.656 Yes 1233 0.448 - 1264 0.391 - 1267 0.410 - 1344 0.475 - 1377 0.548 Yes 1414 0.331 - 1441 0.290 - 1491 0.560 Yes 1500 0.571 Yes 1656 0.453 - 1671 0.528 Yes 1764 0.436 - 1805 0.503 Yes 1815 0.246 - 1846 0.062 - 1872 0.077 - 1879 0.079 - 1919 0.053 -

Explain the output -- Processing locus 251: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGGACATGGATCTCTGTCTATAAATAACGGGAAATCTAGACTTTAAGTATACGAATTTGAGAAATTTCGATTCTTTCGC TTGTTTTGTTGATGGGTGTGAGTTTTTATGCTTAAATTCTTTTTCGTTGCAGGGAAACTTGAGAAGGAGAAAAAAATGTC GAACATAGACATAGAAGGGATCTTGAAGGAGCTACCTAATGATGGGAGGATCCCAAAGACGAAGATAGTTTGCACATTAG GACCAGCTTCTCGCACTGTTTCCATGATCGAAAAGCTTTTGAAAGCCGGTATGAATGTGGCTCGCTTCAACTTCTCACAT GGAAGCCATGAATACCATCAAGAGACACTCGACAACCTCCGCTCTGCTATGCATAATACCGGCATTCTCGCTGCTGTCAT GCTTGATACTAAGGGGCCTGAGATTCGTACTGGTTTCTTGAAAGATGGGAACCCTATACAACTGAAGGAAGGTCAAGAGA TTACTATAACCACTGATTATGACATTCAAGGAGACGAATCAACGATATCCATGAGCTATAAAAAGCTGCCTTTGGATGTG AAGCCCGGAAACACCATACTCTGTGCAGATGGAAGCATAAGTCTAGCTGTCTTGTCATGTGATCCTGAGTCTGGAACTGT TAGGTGCCGGTGTGAAAACTCGGCGATGCTTGGTGAAAGAAAGAATGTGAATCTTCCTGGCGTTGTTGTTGATCTTCCCA CTTTGACAGATAAGGATATTGAAGATATTCTCGGTTGGGGTGTTCCGAACAGCATTGATATGATTGCTCTTTCGTTTGTC CGTAAAGGTTCGGATCTTGTTAATGTCCGCAAGGTTCTTGGATCTCATGCTAAAAGCATAATGCTCATGTCAAAGGTTGA GAACCAGGAAGGTGTGATTAACTTTGATGAGATCTTGCGTGAAACAGATGCGTTCATGGTTGCCCGTGGTGATCTCGGGA TGGAGATTCCGATAGAGAAGATCTTCTTGGCTCAAAAGTTGATGATCTACAAGTGTAACCTTGCGGGTAAACCGGTGGTC ACAGCCACTCAGATGCTGGAGTCAATGATCAAATCACCTCGGCCAACCCGAGCTGAAGCCACAGATGTTGCAAATGCTGT TCTTGATGGTACTGACTGTGTGATGCTTAGCGGTGAGAGTGCAGCAGGAGCTTATCCGGAAATAGCTGTGAAAGTCATGG CTAAGATCTGCATTGAAGCCGAATCCTCGCTTGATTACAACACAATCTTTAAAGAGATGATCCGAGCAACTCCACTTCCA ATGAGCCCACTCGAGAGTCTTGCATCATCCGCTGTACGGACTGCTAACAAAGCGAGGGCAAAACTCATCATTGTGTTGAC ACGTGGAGGTTCAACTGCTAATCTCGTGGCTAAATACAGACCGGCTGTTCCGATTCTGTCAGTGGTTGTCCCGGTTATGA CCACTGATTCCTTTGACTGGTCTTGTAGTGACGAGTCACCTGCAAGGCATAGTCTGATATACAGAGGTCTAATCCCTATG TTGGCTGAAGGATCTGCAAAGGCAACAGATAGTGAAGCCACCGAAGTTATCATTGAAGCTGCTCTGAAGTCGGCTACTCA GAGAGGACTGTGCAACCGTGGTGATGCAATCGTGGCGCTGCACCGTATTGGAGCTGCCTCAGTTATTAAGATCTGTGTGG TTAAGTGAGATTACAGACTTCTTTCAATACCTCAAATCTTGGATTGTTGGTAATCGTAACTGAGATTTTGCTTTGTAGCA TGAAATAAAGAAAACAGGTCACAATAG ......N......................................................................... ...........N...............N...............................................i.... ......................................N..N...................................... .......................N..........................i...N.......................N. .......N........................................i.............................i. ............................................N................................... ..................i...............................i........................N.... ............................i...........................N....................... .........................i..................N................................... ...........................................................i.................... ......................N.......................N.............i.....N............. ..........................N....................N.......N.......................i .........................................i...................................... ............i...........i.......................................N........N...... .....N................i.....................................................i... ........................................................i....................... i............................................................................... ............................................................................N... .............................................................................i.. ................................................................................ .......................N........................................................ ...............................................................................N ........................... Pos Score Pred ------------------------ 7 0.334 - 92 0.437 - 108 0.432 - 156 0.740 Yes 199 0.204 - 202 0.219 - 264 0.479 - 291 0.686 Yes 295 0.300 - 319 0.487 - 328 0.291 - 369 0.821 Yes 399 0.583 Yes 445 0.427 - 499 0.502 Yes 531 0.594 Yes 556 0.367 - 589 0.525 Yes 617 0.350 - 666 0.799 Yes 685 0.379 - 780 0.772 Yes 823 0.244 - 847 0.055 - 861 0.741 Yes 867 0.445 - 907 0.061 - 928 0.203 - 936 0.345 - 960 0.602 Yes 1002 0.669 Yes 1053 0.628 Yes 1065 0.602 Yes 1105 0.252 - 1114 0.319 - 1126 0.101 - 1143 0.748 Yes 1197 0.670 Yes 1257 0.591 Yes 1281 0.655 Yes 1437 0.328 - 1518 0.664 Yes 1624 0.176 - 1760 0.033 -

Explain the output -- Processing locus 252: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATCTGCATGTACCCTTTTACTCTATCATTGTCTCAAAAATGGCTCCTTTTACTTTGGGTATTGATCTGTATGTGTAAA ACGTTGACCCTTTTACTTTAACTCTTGTATGAAATGCGGATTTTGAATCGAAAGATGTCAGACGATTTCGAGAATTCGGT GATAAACTTGAAGCTGCTCATAGATGAAGAGAAAAACAAAGTTGTATTCGCTGAGGCGGGTACAGATTTTGTCGATATAC TCTTTAGCTTTTCGACTTTGCCTATGGGAACAATTGTTAGATTGCTGGAGATGCATCACAAGTCACGCTCAATAGCCATT GGTTGTTTCAACAACATCTATGCAAGTGTTGTGAGTATGGGAATGAAGCATTTCAGTACTCAAGCTTGCAAGCAAATGCT TTTGTTTCCTGGGAGTTTGAATCAGGACAAATGTCAAAATATGAAGCTGAAGATAGATGATTCCGAGGCTACCAAGTGTT TCATGTGTCCTATGTTTGTGCGAAGCGGACAATGCAGCAAAGGCTATAGCAATTTTAAGACCTCTAGGTGTAGCTGTGGG AATTTCATGGATGAGGTGATTCAGTTTCAAGGAGAAGGTGGCCGAGGAAATGGTTCTCAAGCTGAAGTATTTGTGAGGGG TGCTCATACTTCATTCATAATCACTGATGATTTGAAAGTGCAAGTTAACTCTGTTGGGTCAACGCTTAATGTTCTTAAAG ATCTTGCCTATGCAGATTGCGATAAGTTGGTTGAAATGATTCTTGAGGTGAATCTCCAAGAGGTAGCGACTTTGCTGGTG TGTTTGTTCACATCGGATACTCCATTGACCGATACATTTCTGAAAAAGAAAAGCTCACATGGTATGAACAGGATTCATAA AAGGCCGAGTCCTTCTCTCAGTGAAGATAGAGAGGAAACAAAAGCTGATCAAACGATAATGCTCAATGCGTATGTTAGGA AGGAGGAAGGGAACATTCTATATGTTGAATGTGGTGACGACTTTGTTGATCTTCTTTTCTCTTTCCTTGCTATGCCTCTG GAATCTGTATGGGACATCTCGGGCAGAGGAATCATCCTTGGATGCATTGGAAACTTGTGCAAAAGCTTCAAGGATTTGAG TAGTGCTTCCGCAACTGAAGCTTCAGCTTTTAAATGTGTTCTTCCTCATTACTACAAGTGCCAGAAGCAGCTGCTAAATG ATGTTGTCACAACCCATCAGCCACCAACTTATTACCGTTTTGTATCGTTCTCTGCGGATCATTTTCGAGAGTACCGTTTG TCTGAAAACTCTGGTAAGCGACTTGTGTATGACTGGGATAAACTCGTGCCTGTGACTTCGATAGATCCGAAAACCGAAGG CAACAACACTGCTGAAACAAGTGGATTTGTGAAGAGAGGGACAAAGTTCATGGTGACAGATGATCTGATCATTAATCCTT CGAACTCAACCTCAACCATTGGATTCTTAAAGGAGAAACAGACTCGACTAGATGAAGTTGAAATTCGAGTGATCAATATG ACAAAAGAAGAGGCCATCAGATTATTGGGAGCTTCAATGGTGACATCCTCTGCCTTAAGCACTGGTCTGCTAGCTATGGA ATCAGCTTCAACAAGTGATCCTAAATGCCGGTTTTTTAAGAAGCCGAAGATTGAGACAGAGACTTGAGAAGCAATGTTCT AACCGAAAGTTCATGTTCTTTGCTAGATTTGATCACTAGAGAATCTTTTGCTTAGCATCTGTAGAATCTGTTTCAGTTAC TGTATT ........N...............................i..............................N........ ............................N....N....................i......................... .......................N........................................................ .......................N..........................N............................. ...................N................i.....i................................N.... ..............................i.........i...............N....................... ..N........N...................N................................................ ......N...N......................................N.............................. ..........................N.........................................N........... .........N.........................i............................................ ..........................................................N....i................ ..........................................................N......N.....N........ .....................N......N..........................................i........ ........N................................N...................................... .................................N...........................................N.. N............................................................................... ............................N................................................... .................................................i.........N.................... ...................................................N.........................i.. ....................................N......................................N.... ........................N................................................N...... ............N................................................................... ...... Pos Score Pred ------------------------ 9 0.109 - 41 0.756 Yes 72 0.119 - 109 0.242 - 114 0.425 - 135 0.748 Yes 184 0.127 - 264 0.403 - 291 0.426 - 340 0.189 - 357 0.525 Yes 363 0.579 Yes 396 0.282 - 431 0.698 Yes 441 0.636 Yes 457 0.275 - 483 0.425 - 492 0.215 - 512 0.194 - 567 0.379 - 571 0.163 - 610 0.139 - 667 0.109 - 709 0.103 - 730 0.154 - 756 0.506 Yes 859 0.350 - 864 0.649 Yes 939 0.396 - 946 0.141 - 952 0.101 - 982 0.227 - 989 0.355 - 1032 0.519 Yes 1049 0.259 - 1082 0.116 - 1154 0.258 - 1198 0.482 - 1201 0.493 - 1309 0.258 - 1410 0.551 Yes 1420 0.183 - 1492 0.155 - 1518 0.511 Yes 1557 0.407 - 1596 0.288 - 1625 0.483 - 1674 0.080 - 1693 0.073 -

Explain the output -- Processing locus 253: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATATTCGAATATCCTATGATCATTTCTATTTGACAACATATCCAATGATTATCATAATAATTATTAAATTTACTAACTA TGTTAAAGAGTAAGTAGTTAAATAAATAGTTTGGTAGTTAGTATCAGTTATCAAACCAGAGAGAATCTTTTAGACTCGAT CAAACAAAGACCTTCCATTACAAAACCTCAAAAGTTGAAGTCAATAAAAATCTCTTACAACCACAACAACTTCTCAGATT CTTCTTCGCTTTGTTGTTCGTTCTCTTCTTCAAGCTCTCTGTCTCTCCAAATGGGCGACACCGGTAGACACAAATGGTTC TCCTTCCACCACCACCGTTCAGCCTCCGCCACAACTATGCCACAACACAACAGCCCCGGCGAAACTCCAACAGAGTTCCT CTGTCCCATCACCGGCTTTTTGATGTCAGACCCCGTCGTCGTCGCTTCTGGTCAAACATTTGAACGTATCTCCGTCCAAG TTTGCCGGAATCTCAGTTTTGCTCCAAAGCTTCACGACGGAACTCAACCAGATTTATCCACCGTAATCCCAAATCTCGCC ATGAAATCAACAATTTTAAGCTGGTGCGATCGGAACAAAATGGAACATCCTCGTCCTCCTGATTACGCTTACGTCGAAGG TGTTGTTCGTACCCGAATGGATTCACTTCCTCCGGGTTCGGGTCATCGGATCGCTAAATCCGAAATCTTACCACCGGTGG CTGAGAATTCAAATTCAAATTCAGATTACGATTCCGTGATGGGAGCGATTCGATCTCGTTCAAGGACTTCATTATCGTCA ACTACTTCTCTTCCACTTCACCAAACCCGACCCATTAATCACTCGACCCGGATCCAAAGCTCATTCTCTACATCGGATTA TTCATCTTTTCCTCCGATGTCACCGGAAGAAGAAGAGATCTACAACAAATTGACAAGTGTTGACACAATCGATCACGAGC AAGGTCTGATTCAGCTAAGGAAGACGACGAGATCAAACGAGACCACGAGAATCTCTCTTTGTACTGATCGGATTCTCTCA TTACTCCGATCTCTGATCGTTTCACGGTACAACATCGTGCAAACAAACGCCGCCGCGTCGATTGTTAATCTCTCGTTAGA GAAACCTAACAAATTGAAAATCGTACGGTCAGGATTCGTTCCTTTGTTGATTGATGTTTTGAAATCGGGATCAACGGAGG CGCAAGAGCATGTAATCGGAGCTTTGTTTAGTTTAGCGGTTGAAGAAGAGAACAAGATGGTGATCGGAGTTCTTGGTGCG GTGGAGCCGCTTCTTCACGCGCTTCGTTCATCGGAGAGTGAGAGAGCTCGTCAAGACGCGGCGCTTGCGCTTTATCATTT GTCGTTGATTCCGAATAACCGAAGTAGGCTGGTTAAAGCCGGTGCAGTGCCGATGATGCTGTCTATGATCAGATCCGGTG AATCGGCGAGCAGGATTCTTTTATTACTGTGTAATCTGGCGGCTTGTTCGGAAGGAAAAGGTGCGATGCTTGACGGGAAT GCGGTGTCGATACTGGTTGGGAAATTGCGAGAAAGTGGAGGAGCTGAGTCAGACGCGGCGGCGCGTGAGAATTGCGTCGG GGCTTTGTTGACGTTGAGTGTAGGGAATATGAGGTTTAGAGGATTGGCGAGTGAGGCGGGTGCGGAGGAGATTTTGACGG AAATTGTGGAGAGCGAGAGCGGAAGCGGGCGGTTGAAGGAGAAAGCTTCAAAGATTCTACAGACATTGAGAGGCGGAGGG AGTGAATTCGGTGAAGGAGCGGAGGCGCGTGAGTGGAACCGTATGTTGGAAGCGAGTGGGCTAAGTCGTTCTCAGTTTCA ACAAGGAGGGCAAAAAGGGGGTTTTGCTTATTCCTCTCAGTTTTAGCTCTATTCAATTTTTTTATTTTCATTTTCTTATC TCTTTTCTTGTTGGTAAATTTTTTTCTCAAATATTTGTTTTTTTTTCACTCTTTTTTGTGACTAA ................N............................N.................................i ................................................................................ ................................................................................ ..................................................i......................i...... ....................................i........................................... ......................i......................................................... ................................................................................ N......................................i........................................ ................i............................................................... ......................................N......................................... ................................................................................ ................i............................................................... ................................................................................ ................................................................................ .....................................................N.......................... .........N..............................................i....................... ................................................................................ ....................................................N..N........i............... .................................................................i............N. ................................................................................ ............................i................................................... ................................................................................ ..........................................N..................................... ................................................................................ ................................................................. Pos Score Pred ------------------------ 17 0.160 - 46 0.457 - 80 0.573 Yes 291 0.872 Yes 314 0.586 Yes 357 0.774 Yes 423 0.577 Yes 561 0.471 - 600 0.757 Yes 657 0.608 Yes 759 0.488 - 897 0.646 Yes 1174 0.160 - 1210 0.214 - 1257 0.732 Yes 1413 0.496 - 1416 0.248 - 1425 0.568 Yes 1506 0.814 Yes 1519 0.151 - 1629 0.565 Yes 1803 0.219 -

Explain the output -- Processing locus 254: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGTTTTCACATGAGTTCAAACAATTCGGTAGAAAAAGGTCAACCATGCACTCTCCTCAAGAAACTTTGTCATTCAAATA AATCTGGTTTGGATCCGTTGACGAAAAAACAAAGGCCCGTTAGGCAAAAGACTGATGATAATGGGCCTGATTAAAAACGG TACCCTTTATTGTCGTTTTGGGATAAGCTGGAATTTTGCCGCTGTGTTTTTTTCTACTTATTTCCGTCACTGCTTTCGAC TGGTCAGAGATTTTGACTCTGAATTGTTGCAGATAGCAATGGCGTCTGGAGCAGTAGCAGATCATCAAATTGAAGCGGTT TCAGGAAAAAGAGTCGCAGTCGTAGGTGCAGGTGTAAGTGGACTTGCGGCGGCTTACAAGTTGAAATCGAGGGGTTTGAA TGTGACTGTGTTTGAAGCTGATGGAAGAGTAGGTGGGAAGTTGAGAAGTGTTATGCAAAATGGTTTGATTTGGGATGAAG GAGCAAACACCATGACTGAGGCTGAGCCAGAAGTTGGGAGTTTACTTGATGATCTTGGGCTTCGTGAGAAACAACAATTT CCAATTTCACAGAAAAAGCGGTATATTGTGCGGAATGGTGTACCTGTGATGCTACCTACCAATCCCATAGAGCTGGTCAC AAGTAGTGTGCTCTCTACCCAATCTAAGTTTCAAATCTTGTTGGAACCATTTTTATGGAAGAAAAAGTCCTCAAAAGTCT CAGATGCATCTGCTGAAGAAAGTGTAAGCGAGTTCTTTCAACGCCATTTTGGACAAGAGGTTGTTGACTATCTCATCGAC CCTTTTGTTGGTGGAACAAGTGCTGCGGACCCTGATTCCCTTTCAATGAAGCATTCTTTCCCAGATCTCTGGAATGTAGA GAAAAGTTTTGGCTCTATTATAGTCGGTGCAATCAGAACAAAGTTTGCTGCTAAAGGTGGTAAAAGTAGAGACACAAAGA GTTCTCCTGGCACAAAAAAGGGTTCGCGTGGGTCATTCTCTTTTAAGGGGGGAATGCAGATTCTTCCTGATACGTTGTGC AAAAGTCTCTCACATGATGAGATCAATTTAGACTCCAAGGTACTCTCTTTGTCTTACAATTCTGGATCAAGACAGGAGAA CTGGTCATTATCTTGTGTTTCGCATAATGAAACGCAGAGACAAAACCCCCATTATGATGCTGTAATTATGACGGCTCCTC TGTGCAATGTGAAGGAGATGAAGGTTATGAAAGGAGGACAACCCTTTCAGCTAAACTTTCTCCCCGAGATTAATTACATG CCCCTCTCGGTTTTAATCACCACATTCACAAAGGAGAAAGTAAAGAGACCTCTTGAAGGCTTTGGGGTACTCATTCCATC TAAGGAGCAAAAGCATGGTTTCAAAACTCTAGGTACACTTTTTTCATCAATGATGTTTCCAGATCGTTCCCCTAGTGACG TTCATCTATATACAACTTTTATTGGTGGGAGTAGGAACCAGGAACTAGCCAAAGCTTCCACTGACGAATTAAAACAAGTT GTGACTTCTGACCTTCAGCGACTGTTGGGGGTTGAAGGTGAACCCGTGTCTGTCAACCATTACTATTGGAGGAAAGCATT CCCGTTGTATGACAGCAGCTATGACTCAGTCATGGAAGCAATTGACAAGATGGAGAATGATCTACCTGGGTTCTTCTATG CAGGTAATCATCGAGGGGGGCTCTCTGTTGGGAAATCAATAGCATCAGGTTGCAAAGCAGCTGACCTTGTGATCTCATAC CTGGAGTCTTGCTCAAATGACAAGAAACCAAATGACAGCTTATAACATTGTCAAGGTTCGTCCCTTTTTATCACTTACTT TGTAAACTTGTAAAATGCAACAAGCCGCCGTGCGATTAGCCAACAACTCAGCAAAACCCAGATT ..........N..................................i.................................. ......................................................N.....N................... ................................................................................ ......................................i......................................... ...............................................................................N ....................N...............................N......N..............N..... ...........i....................................N............................... ..................................N.............N............................... ......................................................N......................... ...i............................................................................ .............................................N...........................i...... ................................................................................ .....................................................N.......................... .............N..N............................................................... ..........................N..........................N..N..........i............ ......N..........N........N..................................................i.. ................................................................................ ..............N..................................i..i........................... ................................................................................ ................................................................................ ........N...........N..........i.................i......N....................N.. ................................................................................ ................N..............i................................................ ..............N................................................. Pos Score Pred ------------------------ 11 0.446 - 46 0.648 Yes 135 0.178 - 141 0.343 - 279 0.904 Yes 400 0.190 - 421 0.145 - 453 0.320 - 460 0.187 - 475 0.289 - 492 0.659 Yes 529 0.091 - 595 0.190 - 609 0.209 - 695 0.285 - 724 0.519 Yes 846 0.384 - 874 0.601 Yes 1014 0.415 - 1054 0.339 - 1057 0.328 - 1147 0.348 - 1174 0.168 - 1177 0.222 - 1188 0.614 Yes 1207 0.238 - 1218 0.432 - 1227 0.329 - 1278 0.515 Yes 1375 0.346 - 1410 0.618 Yes 1413 0.596 Yes 1609 0.209 - 1621 0.485 - 1632 0.727 Yes 1650 0.806 Yes 1657 0.310 - 1678 0.366 - 1777 0.258 - 1792 0.500 - 1855 0.102 -

Explain the output -- Processing locus 255: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTTGACTTATAGTAGTAGTCAATCCTCTGTTTCACGTAACAGAGAGAAGAAGAAAATATTCATAATAATTAGGAGATTC ATGATCTGAAACAAAAGCTGAGGAAATCGAACAATGTCTTCTGGTGCCTCTTTCATCTTCCTTTTCTTCTTCCTCACTAG CTTCACAGCTTCTGTTGAAAATGTTTTCTATATAAAGCATATTTGCCCAAATACGACAACTTATTCAAGAAACAGCCCTT ACTTGACTAATCTCAGAACCCTTTTGTCTTCTCTTTCTGCCCCCAACGCCTCTTACTCTACTGGATTCCAAAGCGCCAGG GCGGGACAAGCCCCTGATAGAGTCACCGGACTTTTCCTTTGCCGCGGAGACGTCTCACCGGCAGTTTGTCGTAACTGCGT CGCCTTCTCCATCAACGACACACTCGTTCAGTGTCCGAGTGAGAGAAAATCCGTGTTTTATTATGATGAGTGTATGCTCA GATACTCTGACCAGAATATTCTCTCGACCCTTGCATACGATGGAGCATGGATCAGGATGAACGGAAATATTTCAATTGAT CAAAATCAAATGAACCGGTTCAAAGATTTTGTGTCGTCTACGATGAATCAAGCTGCCGTCAAAGCGGCGAGCAGTCCTAG AAAGTTCTATACGGTAAAGGCCACTTGGACAGCACTCCAGACGTTGTACGGCCTGGTTCAGTGCACTCCTGATCTTACAA GACAAGACTGCTTCAGCTGTCTGGAAAGTTCCATCAAATTAATGCCTCTTTACAAAACTGGAGGAAGAACTCTTTACTCT AGTTGTAATTCAAGGTACGAGCTTTTCGCGTTCTACAACGAAACTACTGTTAGAACACAACAAGCACCACCACCGCTACC TCCTTCGTCTACTCCTCTGGTGACATCTCCTTCACTACCAGGGAAAAGTTGGAATTCAAATGTGTTAGTGGTTGCAATTG TTCTGACCATCCTTGTGGCTGCTCTACTTTTGATAGCTGGTTATTGTTTTGCAAAAAGGGTTAAGAATTCTTCTGATAAT GCACCAGCCTTTGATGGAGATGATATAACAACCGAGTCGCTGCAACTTGATTATAGAATGATTCGAGCTGCAACAAATAA GTTTTCAGAAAATAATAAGATAGGTCAAGGTGGATTTGGTGAGGTTTACAAGGGTACATTTTCGAATGGAACTGAAGTAG CGGTTAAGAGACTGTCAAAATCTTCAGGACAAGGTGACACAGAGTTCAAGAACGAGGTTGTTGTTGTTGCAAAGCTTCAG CATAGAAATCTGGTTAGGCTTCTAGGATTTTCTATAGGAGGAGGAGAAAGGATATTGGTCTACGAGTATATGCCCAACAA AAGCCTTGATTACTTTCTCTTTGATCCTGCGAAGCAAAATCAGCTGGACTGGACTCGACGATACAAGGTCATTGGAGGGA TTGCTCGAGGAATTTTATATCTTCATCAAGATTCAAGGCTCACAATCATACACCGTGACCTCAAAGCAAGTAACATTCTC CTGGATGCGGATATGAATCCCAAACTTGCTGATTTTGGACTGGCAAGGATCTTTGGAATGGACCAAACCCAGGAAAACAC AAGCAGAATAGTTGGTACCTTCGGTTACATGGCTCCTGAATATGCAATACATGGCCAGTTCTCGGTGAAATCTGATGTGT ATAGCTTCGGAGTGTTGGTGCTTGAGATTATAAGCGGTAAGAAGAACAACAGCTTTTACGAGACAGACGGCGCACATGAC TTGGTCACACATGCTTGGAGGCTTTGGAGTAATGGAACAGCATTAGACCTCGTGGATCCAATTATTATAGATAATTGCCA AAAGAGTGAAGTGGTTCGATGCATCCATATATGTCTTTTATGTGTTCAAGAAGATCCTGCAGAGCGTCCGATCTTGTCGA CCATTTTCATGATGCTCACAAGTAATACTGTGACTTTACCAGTGCCTCTACAACCAGGGTTTCCCGTTCAGAGTTGAACT GAAAAGGACCCACTTCAACCAACGACCACCAAGTCTGTTCCAAGATTTATTGACGATACATCAATCACACTGATTTATAT CCTCTTTGAACATGGT ................................................................................ N................................i.............................................. ....................i........................................................... ................................................................................ ................................................................................ ..............................................................i..N.......N...... .......................................N......N.........i....................... .........i................................N..................................... ................................................................................ .........................................i...................................... ................................................................................ ...........................................................N.................... ..............................................................................N. .............N.....N.....................................N...................... .................................................................N.............. ................................................................................ .....................................................................N.......... ................................................................................ ................................................................................ ....N.......i............................................i...................... ............................i............N........N.......................N..... ...........................................................................N.... ..........N....................N................................................ ..................i...........i........i........................................ ........i..N.................................................................... ................................................................................ ...........N.... Pos Score Pred ------------------------ 81 0.119 - 114 0.728 Yes 181 0.513 Yes 463 0.585 Yes 466 0.438 - 474 0.391 - 520 0.200 - 527 0.417 - 537 0.819 Yes 570 0.785 Yes 603 0.256 - 762 0.682 Yes 940 0.134 - 1039 0.107 - 1054 0.133 - 1060 0.117 - 1098 0.469 - 1186 0.128 - 1350 0.370 - 1525 0.209 - 1533 0.764 Yes 1578 0.621 Yes 1629 0.796 Yes 1642 0.239 - 1651 0.478 - 1675 0.233 - 1756 0.414 - 1771 0.138 - 1792 0.197 - 1859 0.603 Yes 1871 0.594 Yes 1880 0.523 Yes 1929 0.568 Yes 1932 0.402 - 2092 0.061 -

Explain the output -- Processing locus 256: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATATAATTTCTTATTCCGCTAACTCAAATGTTTATTATTTTTAACATCAAAATGTTTCTAATACTAAAAAGTTTAATA AATAAAAAAATCTCTCTATAAATAGATAAATTATATCGTATAATGTTCAAAACAATTCCCACACTATCTTTCCTCCACAT TAAAGTAAAGTATCTCTCTCTTTTTTCTCCTACGTACTCCATAGTGATGGCTTCATCGCTTCTGACATCTTCCGTTATGA TCCCTACCACCGGTTCCACCGTGGTTGGCCGGTCAGTGTTACCCTTTCAATCTTCCCTGCACTCTCTCCGCCTGACCCAT TCGTACAAGAACCCCGCATTGTTCATCTCATGTTGCTCTTCTGTGTCCAAAAATGCGGCAACTAGTTCTACTGATCTCAA ACCCGTTGTGGAACGGTGGCCGGAGTACATACCGAACAAGCTTCCCGACGGAAACTATGTGCGTGTATTCGACACGACGC TCCGTGACGGTGAACAATCTCCTGGTGGATCCCTCACTCCGCCGCAGAAGCTAGAGATTGCCCGACAGCTCGCTAAACTC CGAGTAGACATCATGGAAGTCGGTTTTCCGGGATCATCTGAAGAAGAGTTAGAAACCATTAAGACCATCGCCAAGACTGT GGGGAATGAGGTGGATGAGGAAACAGGTTACGTCCCTGTGATATGCGCCATAGCTCGATGCAAACATAGAGACATTGAGG CGACTTGGGAGGCGCTGAAGTACGCGAAGAGGCCAAGGATACTCGTATTCACATCTACTAGTGACATTCACATGAAATAT AAGTTGAAAAAGACTCAAGAAGAAGTGATTGAGATGGCCGTGAGTAGTATTAGGTTTGCTAAAAGCTTGGGCTTCAATGA CATCCAGTTTGGGTGCGAAGATGGCGGCAGGTCGGACAAGGATTTCCTATGCAAGATTCTAGGAGAAGCCATAAAAGCCG GTGTTACGGTGGTGACCATCGGTGATACGGTAGGGATCAACATGCCACATGAATACGGGGAACTCGTGACTTATCTCAAA GCAAACACCCCTGGAATTGACGATGTTGTCGTCGCTGTTCATTGTCACAACGACCTTGGTCTTGCAACCGCCAACTCAAT CGCCGGTATACGTGCGGGAGCAAGACAGGTCGAAGTAACTATCAACGGAATTGGTGAAAGAAGTGGCAATGCGTCGCTTG AGGAGGTCGTGATGGCTTTGAAATGTCGAGGGGCATATGTGATCAATGGGGTTTACACAAAAATAGACACACGCCAAATC ATGGCTACCAGCAAGATGGTTCAAGAGTACACGGGCTTGTATGTTCAAGCACATAAGCCCATAGTTGGAGCGAACTGTTT TGTTCATGAGAGCGGCATTCATCAGGATGGAATATTGAAAAATAGGAGTACTTATGAGATCTTATCACCAGAAGATATTG GGATTGTAAAATCTCAAAATTCCGGCCTTGTTCTTGGAAAGCTTAGTGGACGTCACGCTGTGAAAGATCGGCTGAAAGAG TTGGGATATGAACTCGATGATGAGAAATTGAACGCTGTCTTCTCACTATTCAGAGATTTAACCAAGAATAAAAAGAGAAT CACGGATGCTGATTTGAAGGCATTAGTAACATCTAGCGATGAAATCTCTTTGGAGAAATTAAACGGCGCTAACGGTTTAA AGTCTAACGGCTATATACCAGTTCCTCAGGTTTCATCGAATGTGTAAACACGTTGTATCTCTTGTATATTTGTGTACCAC AGTAATATCAATTTCCATTTGATAATGCCGTTTTTGTTGTAATGTATTAAAAACAGTATTAAATAA .............................N.......................N.......................... ..........................................i..................................... ..............................................i.............................N... ................................................................................ .............................N......................i........................... ........................................................N....................... ................................................................................ ............i................................................................... .....i........N...........................N..............N...................... .......................................................................N........ .................................i..........................................N... ....................i...........................N............................... .........................................i......N............................... ......................i......................................................... ....................................................................N........... ...........i..........N.............N........N.................................. i..............N........................N....................................... .....N....................N..........................N.......................... ................................................................................ .......N........N..N............................................................ .....N................................N......................................... .......................................N........................................ ........................N................N........................ Pos Score Pred ------------------------ 30 0.198 - 54 0.091 - 123 0.542 Yes 207 0.763 Yes 237 0.273 - 350 0.299 - 373 0.587 Yes 457 0.446 - 573 0.836 Yes 646 0.567 Yes 655 0.451 - 683 0.364 - 698 0.395 - 792 0.149 - 834 0.802 Yes 877 0.160 - 901 0.573 Yes 929 0.433 - 1002 0.641 Yes 1009 0.314 - 1063 0.517 Yes 1189 0.191 - 1212 0.574 Yes 1223 0.392 - 1237 0.136 - 1246 0.217 - 1281 0.673 Yes 1296 0.488 - 1321 0.247 - 1366 0.260 - 1387 0.148 - 1414 0.267 - 1528 0.177 - 1537 0.077 - 1540 0.107 - 1606 0.169 - 1639 0.191 - 1720 0.089 - 1785 0.047 - 1802 0.043 -

Explain the output -- Processing locus 257: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATAACAAAGCTTTAGACGACGAAAGTTAGTTCCTTTTTTTTTCATAGTTCGTTATGTTCTTTTTTTACCGGTCAACGCT TGAAAATTTTCTAATGTGTAAAACTCCGGCGAAGAAATAAAATTTTTACCGGTTGCTTTGTTTCTTCTCCGGTCACTTAT TGTTAGTTTAATGCTTCTTAACTGTAATTTTCACAATCTCAAGATTAAGAACAGATTAAAAAAAATTCAAGATCAAATAA ATGAAACTGAACCAGAGAGAGACTTGAGGTTAACACGTCAAGATATGGCAGTGTTCTTCATCTTGGTCTTGGTCTTTTTC TTCCTTGTCTTCAGATTCATCTACAGTAATATATGGGTTCCGTGGAGAATCCAATCTCACTTCAAGAAGCAGAGCGTGAC GGGTCCTAGTTACCGGATATTCAGTGGAAACTCCGGCGAGGTTAGCCGGCTAACGGCGGAGGCGAAGTCAAAGCCGATTC CTTCCGGGAGAAATCCTCATGAGTTCGTCCACCGTGTGGCGCCTCACTACCACGAATGGTCACGTGTCTACGGGAAGACG TTCCTCTACTGGTTCGGGTCGAAACCTGTGGTGGCTACGTCGGATCCAAGACTAATCCGGGAAGCTTTGACGACGGGTGG GTCGTTTGACCGAATTGGGCATAACCCTTTGTCTAAGCTGCTTTATGCTCAAGGTCTTCCTGGTCTTAGAGGTGATCAAT GGGCTTTCCATAGAAGAATCGCCAAACAAGCTTTTACAATGGAGAAACTCAAGAGGTGGGTGCCACAAATGGTAACAAGT ACTATGATGTTGATGGAGAAGTGGGAAGATATGAGAAACGGAGGAGAAGAGATTGAGTTAGAGGTACACAAAGAGATGCA CAATTTGTCTGCAGAAATGTTATCAAGAACAGCTTTTGGGAACAGCGTGGAAGAAGGGAAAGGAATCTTTGAATTGCAGG AGCGTATGATGCGTCTTTTTTATCTGGTTCGATGGAGCGTTTATATCCCAGGATTCAGATTCTTTCCTTCGAAGACGAAT AGAGAGATATGGAGAATTGAGAAGCAAATCCGTGTTTCTATCTTGAAACTGATCGAAAACAATAAAACAGCTGTTGAGAA ATCAGGAACTCTGCTTCAGGCTTTTATGTCTCCTTACACAAATCAGAATGGTCAAGAAGAGAAGCTTGGGATAGAAGAAG TTACAGATGAATGCAAAACGTTCTACTTTGCAGCTAAGGAAACAACTGCTAACCTTATGACGTTTGTTTTAGTCCTCTTA GCTATGAACCAAGAATGGCAAAACATTGCTCGGGAAGAAGTCATATGCGTTCTCGGACAAACCGGGCTTCCTACTCTAGA CATCTTACAAGACCTCAAGACTCTAAGTATGATAATCAATGAGACTCTCCGGCTCTACCCTCCAGCAATGACATTAAACC GTGACACTCTTAAAAGAGCTAAGCTAGGAGACCTTGACATCCCTGCAGGAACTCAACTCTATTTATCAGTAGTCGCGATG CACCACGATAAAGAAACATGGGGAGACGACGCAGAGGAGTTTAATCCGCGTAGGTTTGAAGATCCTAAGAAACAATCAGC TCTGCTTGTACCGTTTGGGTTAGGGCCTAGGACTTGTGTGGGACAGAATCTTGCTGTGAATGAGGCTAAAACTGTTCTGG CTACGATCTTGAAGTATTACAGTTTCAGGTTGTCTCCAAGTTATGCTCATGCTCCTGTTTTATTTGTGACGTTGCAGCCT CAAAATGGTGCTCATCTTCTCTTTACTAGGATATCAAGTTAAAAAATACCAAGATTGGCTGGTATTATGGTTTCCGGTTT TCTCCGGTTTTAATGTAATTCGGTTCGGTATTTGAATGAAAAGGCTAAAATGGTCAAAGCA ......................................................N......................... .............N.................................................................. ..........N..................................................................... N...........................................N................................... ................................i............................................... ................................................................................ ..................N....................................i........................ ................................................................................ ............................................N.................................i. ......................................i.............................N........... ...N..N.....N.................i............................................N.... ................i............................................................... .....N..N......................N................................................ ........i....................................................................... .........................N.....................N................................ ......N...N.............................................i....................... ...N..........i.............................i................................... ............................i.........N............................i............ .............................................................................i.. .................i.............................................................. ...........................................................N.................... ..........................................N.....N............................... ....N.............................................................N............. ............N......................N.............N........... Pos Score Pred ------------------------ 55 0.216 - 94 0.177 - 171 0.286 - 241 0.169 - 285 0.327 - 353 0.737 Yes 499 0.251 - 536 0.755 Yes 685 0.172 - 719 0.801 Yes 759 0.667 Yes 789 0.238 - 804 0.296 - 807 0.158 - 813 0.288 - 831 0.514 Yes 876 0.212 - 897 0.528 Yes 966 0.218 - 969 0.375 - 992 0.455 - 1049 0.607 Yes 1146 0.497 - 1168 0.159 - 1207 0.258 - 1211 0.302 - 1257 0.524 Yes 1284 0.297 - 1295 0.705 Yes 1325 0.638 Yes 1389 0.599 Yes 1399 0.234 - 1428 0.522 Yes 1518 0.562 Yes 1538 0.640 Yes 1660 0.197 - 1723 0.156 - 1729 0.073 - 1765 0.309 - 1827 0.173 - 1853 0.050 - 1876 0.038 - 1890 0.085 -

Explain the output -- Processing locus 258: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCCTGATTCCAGTAAAATGATACTTCTCTTTTTCTTCTACTATTTTTTGGGCACATTCTAGTAGTTTGACATTTTAATAA AATTTGGTTTGGATTTATTAGATGTTGCTCTCAGATATGGCTTATGAAGATGACGATGAGACACTTTTGAATGAAGAGGA GGTTGAATCTCAACCAGACATCGATCAGGCACAAAATGATAAGGAGTGGAATTTAAGAGCATGCAGTGCAAAATTTATTG GTATTCTCGCTAATGTATTTGGAGATGAAATTCTTCTAACACTCATGCCTCTTATTGAGGCAAAACTATCAAAATTCGAT GATGAGACTTGGAAAGAGAGGGAAGCTGCAGTGTTTGCTTTTGGAGCCATTGCTGAAGGATGCAATAGTTTTTTTTACCC TCATTTGGCTGAGATTGTAGCAATCCTTCGTCGTCTTTTAGATGATCAATCTCCTCTCGTACGAAGGATAACGTGTTGGA CACTTTATCAATTTGGCACATATGTTTTTGAGGAAAGTAATTTGGAGAATAGTAAACTCTTTACAAAAGTTCTTCATGGT TTTCGTTTCAAACTTTTGGATTCGAACATCTGGGTCCAGGAAGCTGCATGTTTGGCTTTGACAACTTTTGAAGAGGATGC TGGTGACAAATTAGTACCACACCTGGAAAAAATATTACAACAGCTAATGCGCGCTTTTGGAAAGTATCAGAAGAGGAACT TAAAAGTTCTTCTTGATGCTATTAGAGCACTAGCAGATTCAGTTGGAATCAATCTGAACAAGCGTGCTTATATTAAGATT TTGATTCCCCCACTGGTTTCAACGTTGGAACAAATTTCCAATTCAGATAAAGATGTAATTCCATTGCTGAAGTGCTTCAC ATCCATTTCTAAGGTTTCACAAAGTAATCTAAGGGATATGCTTCTTAAGTGTTTCATGGATGAAACTCCAGATGTTAGAG AAAGTGCTTTTGCCCTTATTTGTCATCTTACAAAAGTACTCCCGGATTATCTAGAACCCCGCCTGCTTGAGTTCCTCGAA ATCGCCAGCCAACAACTGAGTGCAAATTTTAGCGGAGAAAACCTTTCTGCTGCGAATAATGCTTGTAAAGCAATAGGAGA ATTAGCGGTCAAGTATCCTCAAGAAGTCTCACCAATTGTGACCAATGTTGTCTATTCATTAGGCATGATTATTCAACTCG GAGAGACACTAGAGTTGAAGAGTTTAACTACACTAGTTGAATACAATGCAATTGAACTAGCTATGAACAGTGCAATTACC GTTGGGATACTAGCACGGATTCGTCCAGACCTCAGTGCACGATCCATTGAGAACTTCATGAAGCCTTGGTGCATGAGATT GGCAACGTTAGATGATGACAGTACAAAAGAAAATGCATTTCAAGGGTTATGTGAAATGGTTAAGGTGAATCCATCAAGAT ATGTCAGCTCCGTTGCTTTCATATGCTTAGCGATCGCAAGCTGGAAAGATATGGAAAACAAGGTCATCCAAAGCGAATTC TCCAAAGTGCTGATAGGCTATAAAAACATGCTTGGAAAAAACTCATGGGAAGAATGTCTCTCTGTTTTGGATCCTCTTGC TAAGGAAAGGCTTGCTGCGAGGTATCAAGTGTGACATTGATAGTAAATCGATTAGTCTCAAATGAGAGAGATCTCATTGC AAACAATATTCAGTGTTTTTGCAAAAGACGTTTCAGTTGCATTATTTTTATAA ................N............................................................... .....................i..............i......N.....N.....N..............N......... ...................................N........................N................... ............N...........N...................N.................................N. .........................................N...................................... .....................i.....................................................N.... ...............................................N............................N... ..............................................i................................. ...............N................................................................ ....................................................N........................... .....................................i.................N...N...........N........ ................................................................................ ..........................................................N..................... ............................................N...................i............... .............................................N................N................. .........................................................N..............N....... ...........N..N.................N...............N......i........................ N.....................N...........................i............................. ...........................N................N........i.......................... .............................................................N.................. ..................................................... Pos Score Pred ------------------------ 17 0.332 - 102 0.684 Yes 117 0.810 Yes 124 0.385 - 130 0.469 - 136 0.175 - 151 0.221 - 196 0.113 - 221 0.471 - 253 0.236 - 265 0.127 - 285 0.422 - 319 0.201 - 322 0.230 - 380 0.289 - 442 0.125 - 502 0.506 Yes 556 0.128 - 608 0.409 - 637 0.366 - 687 0.623 Yes 736 0.076 - 853 0.251 - 918 0.527 Yes 936 0.283 - 940 0.066 - 952 0.428 - 1099 0.198 - 1165 0.166 - 1185 0.562 Yes 1246 0.281 - 1263 0.405 - 1338 0.193 - 1353 0.239 - 1372 0.340 - 1375 0.451 - 1393 0.459 - 1409 0.397 - 1416 0.679 Yes 1441 0.305 - 1463 0.212 - 1491 0.502 Yes 1548 0.451 - 1565 0.361 - 1574 0.563 Yes 1662 0.097 -

Explain the output -- Processing locus 259: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGCTAGCGTTGCGTCTCTTCTTGATCATCATCGTCGTCACCCCCAGCCTTCCCTTCAACAACAAGTAAGTCAAACTCGTA TTTATAATCCTATCATATTAATTTGGAATCCCAACAATGACATATGGAAACCTAAGTTATATCATATTATCGGTTTTGTT GCAGCCATCAAAAGTGAATGAGCATAAGAGCGAAGAAGAGAAGAATGATTTGATCAAGAAGAAGCAAAAGCGAAGTCTTC AAAGAGAAGTGATGAAAATGCAAGGAGAGCTAGAGGACGAACAAGCCTTAAACAAGGCTCTACGTGATATGCATCGTGGT CCCGTCATGTCTCAGCCTCGTCTCTCTTTATTGCTTCTTCCTCCTCAGGTTCAAGAGCTAATAGAGGAGCTTGCGACGGT TGAGGCAGAGATACTCTGTCTTGAGAAACGAATCCAAGACTTGAAACTTGATGTGTATAGTGAGAAAAAAGAGAACAAAG AACTTGAAGACAGTATTGATGAAGGAGAAGAAGAGAGGATGATGAACCCTAAGAGACTACTACAAAGGCAGAATCATCTT CCTTGCGACGCTGACAATGACCTTATAAAGATGAGATCTGAGGATCTAAAACAGAGATCAAAGTCCCAGAGTTATGGAGA TCATCATGTTGTCAAGGATATTCAAATGAACAGTCCACGTACTCATGCGTCAATTGGATCAACCATGGAGTTCTCATCAA GAATCCATTCTTCGACTTTTTCTGATGGAATGTCAAGAACACAAGAGAAGAATAATGTGCAAGAGACGACACCAAATGGA GTATCGGAAGATCTGGTGAAGTGCTTGATGGGAATCTATCTGGAACTCAACAGGTCATCGCGTGAGCGAGAAGGGTCAAA AACTGTTTCAAAGCTGAGCCTCACACACCTCAAGAACGCTTCTTTCAAACGCAAATCGGTGTATGACCATAACGCATCTA ATCTTGATCCATATGGAGCTGTCATGGGGACTTCTCTTAGAGACATAGGAGAATACAAGAACTTCATCCATATCACTCGA ACCTCCATTGATGTTAGCCGTTTATCCGATTGCTCCACTTCTCTTGTCAATTTGAGGGTTTTGAAGGAGAAGTTGAGCAA AGTGGATTTGAGTTTCTTGAACCACAAGAAAAAGATGGCATTCTGGATCAATACCTACAACGCCTGCGTTATGAATGGAT TTTTGGAACACGGACTACCTTCATCAAAGGAAAAACTGCTGACCATTCTCAAAATGGCAACAATTGACGTGGGAGGTACA CAACTGTCTGCTCTAGATATCGAAGGATCCATCTTGCAGAGTCCATGCGAACCTAGGGAATCTGTTTCAGCCGGTGAAAG CGAAGTGAGAATACAGACACGCTACGGATTCAGATGCGTAGAGCCAAACCTAATGTTCGTGCTTTGTCGTGGAGATTGGT CTTCACCTGCATTGAGAGTTTATACAGCAGAAGATGTGGTGAACGAACTAATCAAAGCAAGAACAGAGTATCTAGAAGCT TCCATCGGTGTCTCGGGAAGGAAAAAGATTGTGATTCCGCGATTCTTGCATAAACGTCTACGGGATTTCGCTGAGGATGA AGGCTCGTTAATTGAATGGATATGCAGCCAATTACCACCAGCCCAACGTTGCTTTCAGCTCAAGGAAACAGCGATGGAGG GGTTGAACAAGAAAAGTGAATCTCAATTGAAAAAACTGATAGAAGTGAGGTCTCACGAATACGAGTTTCGTTATCTCTTG GCATTGTAAAATCTAATCCTTCAAATTTCTAGTATTTGGAGCCTTGAAAAGACGATTGTCCTATATATGTGTGTGTGTTT TAAGAGAATGAAGTTACACAGTGAATTT ................................................................................ ....................................i......N.................................... .................N..........................N................................... ...........N.....N..................................................i........... ......i......................................................................... ..................................................N............................. ..................N...................i..i...................................... ................N.............i..........................................N...... .....N...................i..................N...................i............... ........................i....i........................N....................N.... ...........................N.................................................... ..............................................................N................. ............N..........i........................................................ ..........N..................................................................... ..................................N...................................N...N..... .....................................................i.......................... ............................................N................................... .................................N..................i........................... .................................N.............................................. ............................................................................N... ...............i.....i...................................................i...... ................................................................................ ..................................................................N............. .......N.................... Pos Score Pred ------------------------ 117 0.801 Yes 124 0.472 - 178 0.419 - 205 0.196 - 252 0.345 - 258 0.231 - 309 0.644 Yes 327 0.572 Yes 451 0.174 - 499 0.251 - 519 0.640 Yes 522 0.651 Yes 577 0.427 - 591 0.702 Yes 634 0.247 - 646 0.179 - 666 0.755 Yes 685 0.315 - 705 0.558 Yes 745 0.509 Yes 750 0.555 Yes 775 0.477 - 796 0.169 - 828 0.241 - 943 0.403 - 973 0.163 - 984 0.592 Yes 1051 0.140 - 1155 0.422 - 1191 0.255 - 1195 0.283 - 1254 0.506 Yes 1325 0.388 - 1394 0.240 - 1413 0.579 Yes 1474 0.340 - 1597 0.269 - 1616 0.765 Yes 1622 0.654 Yes 1674 0.581 Yes 1827 0.047 - 1848 0.057 -

Explain the output -- Processing locus 260: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTTACACATCGCCATCTGTTCATAAATTGCATATATCTTTGATTTTAAGCATCACTATCTGAGTCTTTATCTCGAGCT TATCCTCACTGACGGCAATAAACGGCAATAAGTTCTTTTGTCTTAATGTTTCTCTATATCAAAAAAAGCAGAGCACAAAC CAGAGGAACGACAATAATGGTGAGTCTTCTATCTTTTTTCTTGCTTCTACTCGTCCCCATTTTCTTCTTGTTAATCTTCA CCAAGAAGATCAAGGAGTCAAAACAAAATCTTCCTCCTGGCCCAGCAAAGCTTCCGATCATCGGAAACCTACACCAGCTC CAAGGGTTGCTTCATAAATGTCTTCACGATCTCTCCAAGAAACACGGACCTGTGATGCATCTCCGTCTAGGGTTTGCCCC AATGGTCGTAATTTCATCAAGTGAAGCAGCTGAAGAAGCTCTTAAAACACATGACCTTGAGTGTTGTTCAAGACCTATCA CTATGGCCTCAAGGGTTTTTTCGCGTAACGGTAAAGACATCGGATTTGGGGTTTACGGTGATGAATGGAGAGAGCTGCGT AAGCTTTCGGTTCGCGAATTCTTTAGCGTGAAAAAAGTTCAATCCTTCAAGTATATTAGAGAGGAAGAGAATGACTTGAT GATCAAGAAACTGAAAGAATTGGCTTCGAAGCAATCTCCGGTGGATTTGAGCAAAATCCTCTTTGGTCTCACTGCGAGTA TCATATTCAGAACCGCCTTTGGACAAAGTTTCTTTGATAACAAGCATGTCGATCAGGAAAGCATCAAAGAACTGATGTTT GAATCTCTGAGCAATATGACTTTTAGATTCTCTGATTTTTTCCCTACTGCTGGTCTTAAATGGTTTATAGGCTTTGTGTC AGGCCAACATAAGAGGCTTTACAACGTCTTCAACAGGGTTGATACTTTTTTTAATCATATAGTTGATGATCATCACTCGA AGAAAGCAACTCAAGATCGTCCTGATATGGTCGACGCTATCTTAGATATGATAGATAATGAACAACAATATGCATCTTTC AAGCTCACCGTTGATCATCTCAAAGGAGTCCTCTCAAATATATATCACGCTGGAATTGACACAAGCGCCATCACCTTGAT TTGGGCGATGGCAGAGCTCGTTAGAAACCCGCGGGTAATGAAGAAAGCTCAAGACGAGATCCGAACTTGCATTGGAATCA AACAGGAAGGAAGAATCATGGAAGAAGATCTTGATAAGCTTCAATACTTGAAGCTTGTGGTGAAAGAAACCTTAAGACTA CACCCAGCAGCTCCTCTTTTACTTCCTCGAGAAACAATGGCTGATATCAAGATTCAAGGCTACGACATTCCTCAGAAAAG AGCTCTTCTTGTTAATGCATGGTCTATAGGACGAGATCCGGAATCCTGGAAAAATCCTGAAGAGTTTAACCCGGAGAGGT TTATTGATTGTCCTGTGGATTACAAGGGACATAGCTTTGAGTTGTTACCATTTGGTTCTGGTCGGAGAATTTGTCCAGGA ATAGCTATGGCGATCGCAACCATTGAATTGGGGCTCTTGAATTTGCTCTACTTCTTTGATTGGAATATGCCTGAGAAGAA GAAAGATATGGACATGGAAGAAGCTGGTGATCTCACTGTTGATAAGAAAGTTCCTCTTGAGCTTCTGCCAGTTATTCGCA TCAGTTTGTAGATGAGCTTGGAGAAAAACATATGGTATGCTTATTGTATAAACCAGAACGCTTTTTCTGTTTATTTTGTT TTTTTGAATCTAATGTTAAATTATATTCAA ................................................................................ .............................................N.................................. ................i............................................................... ................................................................................ .................N....................................i......................... ..N.........................................................N...i............... ......................................................................N.......N. ................................................................................ .............................................N............................N..... ...............N...........................................N.................... .................................................................N.............. ..........................N....................i.........N...........N.......... ................................................................................ .......i.............................N.......................................... .................i.............................................................. ....................................i........................................... ..............N...N............................................................. ................................................................................ ......i...........................................................i............. .......i.....N.................................................................. ...........N...................N....N........................................... ............N................. Pos Score Pred ------------------------ 126 0.107 - 177 0.556 Yes 338 0.366 - 375 0.823 Yes 402 0.513 Yes 451 0.291 - 483 0.413 - 541 0.277 - 545 0.567 Yes 631 0.220 - 639 0.408 - 766 0.120 - 795 0.483 - 816 0.253 - 860 0.321 - 946 0.069 - 987 0.471 - 1008 0.729 Yes 1018 0.277 - 1030 0.238 - 1128 0.734 Yes 1158 0.211 - 1218 0.688 Yes 1317 0.780 Yes 1375 0.487 - 1379 0.241 - 1527 0.813 Yes 1587 0.620 Yes 1608 0.826 Yes 1614 0.440 - 1692 0.109 - 1712 0.122 - 1717 0.083 - 1773 0.034 -

Explain the output -- Processing locus 261: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAATCAATTATACAAATGACACAAATTTAATATTTGTCGTGATTGTCATTTTCAGGTTATTAACTTGCATGACCACACC ATGTCGTTTCTAGCATAATAATATAAGAGTAATTGTTGGAGACCTTTGTCTTCTCTTGATCTTCTCAGACGGCAGTTCAT AACAAAACTCCTAATTAAGAATTTGGTAGTTGGATCCCAGGTACTTTTTTATATGCACACGATTCTATTATACAATGGAC TCTGGCTGATCGAATTGTCCGGTGTACACGCAGGTTTGCATACCGATCCGGGGATGGATTTCTTCCATTCAAGAGAGAAG AGACATGGCAAAAGAGCAGGATTGTTTGGTCACAAAAGTGCGAGCAAGAGCAACCCGTCGAGCCCTCCTCATCTTGCTGA GGGTCGGTCTCAGCCGACAACTCATTTCGGCATGAAAAGATCGGAATCAGAGTACGCGTTTCCCATCTCCGATGAACAAA CCACTCACTGGAAACCACAACAGCAAGCCTCTGAACGCATCCCAAATTCCCACCAACGGCCTCCAGTCTACAGATATAGC ACGCCTGAGCGTCCTAGAGAGCCTGATGAGGATGTGACTCCAAGGAGCAACGGTTCTGGGAGCCCTTTTCGATCTGCCAG AACCCGCACGCCTGATCGTCGTAAAAGGTCTAGCGAGATAACCAAAGAGCTGTACGAGAGAATGTATGAGGCAGATGCCA ATCATGAACGTGGAAGAGACTACAGTAGAGAAAGATATGAAGCTGAAGGCAATGTAACTTCCCGGAACAGACCTTCAAGC CCATTTCATCCATCCCAAAGCCGCTCTCCACCGCCACACGCAAGGACTCAGAGATACAACAACGGAAAAGATCATTTCGA GGGGATGTACGAGGCAGATGCCGATATCACACCGCGTAACAGCCCTCCCATGAGTCCTGTTCATCAACACACAAGCTACT CTCCACCACCGCCGTTCTACTCATCCAGTGACGACGAAGACGACAACAATTCCACCTATCTCTTTCCAGAAATTGCCACT GGACGTCGTTCCAGGGGAGTTTCAGGAAGCAGCACGCCGGTTCACTACAAGTATCAGATCACATCGGTAGAGACTTACGA GCAGGAGAGGCAATTCGAGCCGCCGGAGCTGCCGGACGAGTCACAGAGCTTCACGATGCAGGAGATCGCCAGAATGCGCG GACTTCAAAGCTACGGAAAGGAAGAGATACAATTAGCGATATCCGAGACTTACGTCTCCGTCGCCAATTACAAGGTACGG ATGAGCATCGCCGCCACGCTCGAAGCGATCATTGACAAACACGGTGACATCGCCGCCTCGTCGAAGTTACAATCTACCTC AACGAGGTCGTTTTATCTCGAATCCCTAGCCGCAGCAGTAATGGAACTGAAATCAACGGCGTTGAGAGATCTGACGAAGA CGCGAGTGGCGGAAATCGCGGCGGTCGTGAAGGACATGGATTCGGTGAAAATCGATGTATCGTGGCTCAAAACAGCGGTG ACGGAGCTAGCGGAGGCGGTGGAGTACTACGGGAAGTACGACACGGCGAAGATTGTGAGGGAGGAGTGCGATAGGGAAAT GACGGCGGGGAAAAAAGAGATGGAGGAGATGAGGGAGGAGCTGCGGCGGAGAGAGAAGGAAACGAAGGAATGTAGAGAGA GAGTGACGGAGTTGGCGGGACGGCTGGGGCAGCTGGAGATGAAGGATGTGAGAGTGAAGAAGAATCTGGAGCTCTATGAG AGCAAGGTCCTTAAATTCGACGGTCATTCGGTTCTCGTTGACTGAACCCGACCTTTTGGAAATTTAATTAAACCGGTTTA GTGGAGTAAACCGCTTTGGAAAATGCTTATCCAAGCATAATCACTCGGAGAAGTCTTTGTTTGT ................N....................................................N.......... N............................................................................... ....................................................N.....................i..... .....................................................N.......................... ....i........................................................................... ...............................N.......................................N........ ................................................................................ .........................N.....N................................................ .............................................................i...N........N..... ...i................................N..............i............................ ................................................................................ ....i............i...............................N.............................. ................................................................................ ................................................................................ .......................................................i.................i...... ................................................................................ i............................................................................... ........................................i....................................... ...................................N..................N......................... ..............................................................................i. ...................i........N........................................i.......... ......................................N......N.............................N.... ................................................................................ ......................N......................................... Pos Score Pred ------------------------ 17 0.354 - 70 0.473 - 81 0.262 - 213 0.259 - 235 0.700 Yes 294 0.266 - 325 0.601 Yes 432 0.390 - 472 0.377 - 586 0.289 - 592 0.328 - 702 0.862 Yes 706 0.376 - 715 0.309 - 724 0.678 Yes 757 0.463 - 772 0.689 Yes 885 0.510 Yes 898 0.514 Yes 930 0.358 - 1176 0.603 Yes 1194 0.522 Yes 1281 0.542 Yes 1401 0.604 Yes 1476 0.470 - 1495 0.149 - 1599 0.622 Yes 1620 0.644 Yes 1629 0.411 - 1670 0.850 Yes 1719 0.398 - 1726 0.487 - 1756 0.135 - 1863 0.053 -

Explain the output -- Processing locus 262: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTTTGAGATCCTTAAGGAAAAAAAATCTTCTCACGTATTTTTTTTTTTTTTTGGTTCTGTTTAAAATTAAAAATAAAA TTTATTTGTTTACAAACTTAAAATATGAAGATTTGGGACTTAAGACATGTGTCAATAGAAAATGTTGCAGTTCCTTGTAA ATCTACTTGTGATTTATTATGTAAGGATTGCTATTTATTTTTAGTTTGTTCCTTGAGCCACCCAACATTATCACGTTCTT TCAATCATAAAACATTTTTTAATGTAATGAACAAAGCAATGCTTGCAAAAATCGTCGTTGACTTTCAAAATTGCCTCATT TTCATTCTTATATCCTTATTTTCTCTCCTTTGCCTCTTTGTATTCGTCTTCAAGAAGCCAAAGGACTCACGAGGCTGTGA TCTGCCTCCGAGCCCTCCTTCTCTTCCGATAATCGGTCATCTTCACCTTATACTCTCTACTCTACCTCACAAGTCCTTTC AAAATATATCCTCCAAGTATGGACCTCTTCTCCTTCTCCGATTTTTCAATGTCCCCGTTGTCCTCAAATCCTCTGCCAAT GTGGCCTACGAGATCTTCAAGACGCACGACGTGAACATCTCGTCCCATGGCCACCCTCCGATTGACGAGTGTCTCTTTTT TGGGTCTTCTAGCTTTGTAGTGGCTCCTTATGGATATTACTGGAGGTTAATGAAGAAGCTCATGGTCACAAAGCTGTTCG GACCTCAGGCACTCGAGCGGTTACGACACGTCCGTGAAGATGAACTAGAAAGGTTTCACACAAACCTGCTTAGTAAGGAA ATGAAAGGCGAGACTGTCCAGATTGCTAAGGAAGCAATAAAGCTGACTAACAATAGCGTCTGCAAAATGATCATGGGTAG GAGTTGTTTAGAGGAGAATGGTGATGCAGCAAGAGTTAGGGGATTGGTCACCGAGACATTTGCCTTGGTTAAGAAAATCT TCTTGACACAAGTGTTGCGTAGGCTGTTTGAAATACTCGGAATCTCACTGTTCAAAAAAGAGATATTGGGTGTTTCCCGC AAATTCGACGAGTTTCTGGAAAAGATTCTTGTGGAACACGATGAGAAACCGGACTTTCAAGGTGGAGACATGATGGACGT GTTGTTGGCAGCTTATCGAGATGAAAATGCAGAGTATAAGATCACTAGGAACCATATCAAGTCGTTGTTCGCGGAGCTTA TCCTTGGAGGCACTGACACCTCAGCGCAGACAATTGAGTGGACAATGGCAGAAATCATTAATAAGCCTAACATTCTTGAG AAATTGAGAAAAGAACTCGATTCTGTTGTAGGAAAAACAAGGCTGATTGAAGAGAAGGATCTACCAAACCTCCCTTATCT GCAATCGGTCGTCAAGGAAGGGCTAAGATTGCACCCACCAGCGCCTGTGTTTGGAAGGAAAGTCCTAGAAGGATGTACGA TTAAAGGCTATTATGTACCAAAGAACACAGCTCTTGTTGTTAATGCTTATGCTGTGATGAGAGATCCCCATTATTGGGAA GATCCTGATGAGTTTAAGCCAGAGAGGTTTTTAACTACTTCAAGCAAAAAAGAAGAGGAGAGAGAGCAAGAACTTAAGTA CATTCCTTTTGGCAGCGGAAGGAGAGGATGTCCTGGAGTAAATCTAGGTTATATATTTGTAGGAACCGCAATAGGAATGA TGGTGCATTGCTTTGACTGGAGAGTCAAAGGAGATAAGGTCAACATGGACGAGACTGCTGCTGCATTGACCTTAAACATG GCCGTGGGACGAAGAGACACGTGTTGATGATGAGCTGGAGAATAAATGAGCAACTTGAACGTTAACGGCGATAGTTGGTG ATGGAAAAGTTGTTAACATAGTTTATACCAAAAATTGTAAACTCTA ................................................................................ ........................N.....................i..............i.................. ..................N............................................................. .....................N....N...........i......................................... ................................................................................ ................................................................................ ..................i.............................N.............................N. ..............................................N................................. .............................N...................N...........i.................. .......................................N........................................ i.................................................................N.....N....... .................N.....N........................................................ ................................................................................ ........................................N............................i..N....... ....................N.....N..................................................... ............................................i................................... ................................................................................ ........................................................................N....... ............N.............................N.....N.......i....................... .......N........................................................................ ...........................i................................................i..i ............................................i................................i.. ..........................N..N...............N.................................. N............................................. Pos Score Pred ------------------------ 105 0.216 - 127 0.757 Yes 142 0.506 Yes 179 0.461 - 262 0.111 - 267 0.197 - 279 0.570 Yes 499 0.549 Yes 529 0.379 - 559 0.243 - 607 0.393 - 670 0.283 - 690 0.354 - 702 0.567 Yes 760 0.345 - 801 0.619 Yes 867 0.441 - 873 0.430 - 898 0.302 - 904 0.139 - 1081 0.140 - 1110 0.574 Yes 1113 0.418 - 1141 0.076 - 1147 0.246 - 1245 0.782 Yes 1433 0.295 - 1453 0.168 - 1483 0.356 - 1489 0.250 - 1497 0.583 Yes 1528 0.283 - 1628 0.628 Yes 1677 0.733 Yes 1680 0.667 Yes 1725 0.764 Yes 1758 0.509 Yes 1787 0.099 - 1790 0.085 - 1806 0.075 - 1841 0.054 -

Explain the output -- Processing locus 263: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTTAACTAATTTCAAGCTTATCACTTCATTTTTCTTTTTGCTAAATCCTGCACATGATTCAATGTTCTTGACCCCATT TGACGTACAAATACAATATAACGTGCATCTATTTATACGTGTATATATATGCAATCAAACCTCAAACACAACCACATAAC AAAAAAAATGATGTCCGAGAAGTTCCCTGATGTATTCACTTGGATACAAAACATCCCACAGATCACCAAATGGCGCACAA CTTCTCTTCCCTTTTGCATTTGCCCTTCAACTTCTGATTTTCCAAATTCAACCCTAAATCTCACAGCACAAAAGAGCCCT AGCCCTAAAGTTGTCACTTTCTCCATCATCGTCCAATCCAATAATCATTCTCCTTTGTATCTTTGGACCACTAAACAAGA GCTCAGCATCAACCCAAACTCACCAAACCCTTTCGATGAACTAACAATCATCTCTCTCCTCTTCAATTTCGTCGAAACTA TCTTAACCTATACCTCTAATAGTTCCAACTATTCAACGATCAAAATCCCTAACTCCGATTCATCCAAGATCGGCGGTCTT AAAGACATAGTCAACACCGTGATTCTCACTCTCTCGTTCGTTGTTTGCGTCTACGAAGCTCCTCTGTATCTCCGTGAAAA CTGCCTCAATACCCTCAAGAACCATTTGATTACTTGTCATACACGGCGAGCTACTATATCTCTGATGAAGCTTCTAGGGT CTAATTTGGAAGAGCAGTGGATGAGAACAGTGAATCTAGCGTTTACAAATTGGATCATCGAGCAGCGTCGTAGTCAGTCA ACCAAGATCACAACAACTCCTTTGTTCTCTTACGCTGTTTCGGCTTATGGATTGTGGAAAGTGCAATTGTATTGTCCCGT TGAAGCCATGGAAGTAGAGAGATCGAGCAACCCAACGGCTGATTCGAGATTGTTGTTCTCTCTCAAGTTTAATCAGCTTG AAGGTGTGATGCAGTTCAATCACAAAGTTGTAGTTAGAGACAATTGGATCGATGTGATCGTGAAGATAGACAACATCAGA TACGATGTAATAAAGCTTGTAAACGAGAAACTGATGTCGAGAAGAGGAGCAGGGGAGCACGAAAAGCATTTCCCATCAAG GATCTCTCTGCAACTAACTCCTACTCTCCAAACCGATTTCATTAGCGTTTCGGTTAGCAAATCATCAAACAATCCGGGAA GAGAGTTTGAAGTCGAGAGATCTATTGAAGGCTCGTTCGATCCACCAAACTCACTAGGCTTAAGAGTTGCTGGACGAGAA GCCTCAACCATGACAATGACCCCGTGGAAATTAGAGCAATCTGTGCTCGGCTACACAGCTAATCTAAACTGGATCTTGTA TGATAGCAGCGTTGGAGGAAGAGAGGTTTTTTCGACAAAGCCGTCACGGTTTTCAATAATGAGCCCAAGGTCTTGGTTTA AGGATAGGTATGCAAGAGCGTATCGGTCATTTACGAGGAGAGGAGGTGTGATATTTGCAGGGGACGAGTATGGAGAGAGT GTGGTGTGGAAGATTGGAAAAGGAGCATTGGGGGGAACAATGGAGTGGGAGATTAAAGGGTTTATTTGGTTGACTTACTG GCCAAATAAGTACAAGACTTTTTACCATGAGACTCGTAGATTGGAGTTTACACAGCTTCTTAATCTCACCATTGCTTAAC TTTAAACAAAAACATGTTATTACTTCTTTGTGATCTCAACTTTACATTGGTAAAGGAAGAGACTTCAATTGTCAGTTAGT TATTTGGTAATTTATCTT .......................................................N.......N................ ................................................N............................... .......N..N..................N.......................................i.......... ................................................................................ ................................................................................ ...................................i............................................ ................................................................................ ................................................................................ ................................................................N............... ....................i........................................................... ..............................................N................................. .......N........................................................................ ........i..........................................N............................ ....N............................N.............................................. ................................................................................ ................................................................................ .........i.....i...............................................................N ..........................................................N..................... .........N...........................................................N.......... .......................................N........................................ ..........................N..................................................... .............N.................................................................. .................. Pos Score Pred ------------------------ 56 0.290 - 64 0.478 - 129 0.415 - 168 0.488 - 171 0.437 - 190 0.466 - 230 0.553 Yes 436 0.540 Yes 705 0.353 - 741 0.531 Yes 847 0.154 - 888 0.312 - 969 0.520 Yes 1012 0.142 - 1045 0.176 - 1074 0.283 - 1290 0.547 Yes 1296 0.784 Yes 1360 0.300 - 1419 0.309 - 1450 0.192 - 1510 0.114 - 1560 0.260 - 1627 0.151 - 1694 0.139 -

Explain the output -- Processing locus 264: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATAATGGAGACGACTTCAGTGGTATTTGTTTAAATTATTTCTTTGTCTTGAAAGATTCTAAAGATGTTTTGATCCAAC GAGTTTTTGCTGAAAAACTGAATGATGGAAACGGATTTCTCGTTGGTGCGTAAGGAGGAGGAAGAAGAAGAAGATAACAG GAATGGGATGAGTTATTTGTCAATGGAGATGATGAAGAAGGTGAGCTCCATGGCGGCTCCAATGGTGGCAGTTTCGGTTT CTCAATTCCTCCTTCAAGTCATCTCCATGGTTATGGCTGGTCATTTGGACGAGCTCTCGCTCTCAGCTGTCGCAATTGCC ACTTCCCTCACCAATGTCACTGGTTTCAGCCTCATCGTTGGGTTTGCAGGTGCGTTAGACACTCTTTGCGGTCAAGCTTT TGGAGCAGAACAATTTGGAAAAATTGGTGCATACACTTACAGCTCAATGCTCTGTCTTCTCGTTTTCTGTTTTTCAATCT CTATCGTATGGTTTTTCATGGACAAGCTCTTGGAGATTTTCCATCAAGATCCTCTCATCTCTCAATTAGCTTGCAGATAC TCAATCTGGCTCATACCGGCTTTATTCGGATTCACTCTTCTTCAACCTATGACTCGGTATTTCCAGTCACAAGGAATAAC TCTTCCTCTCTTCGTGAGCTCTCTTGGAGCTCTTTGTTTTCACATTCCTTTCTGTTGGCTTCTTGTCTATAAACTGAAAT TTGGAATAGTTGGCGCCGCTTTGTCCATCGGTTTTTCATATTGGCTCAACGTATTTTTGCTTTGGATTTTCATGAGATAC TCTGCTTTGCATCGTGAAATGAAGAATCTTGGTTTGCAAGAACTCATCTCGAGCATGAAGCAGTTCATCGCCCTCGCGAT CCCCTCTGCAATGATGATATGCCTGGAATGGTGGTCGTTTGAGATTCTACTACTAATGTCTGGACTCTTACCAAACTCAA AGCTCGAAACATCGGTGATATCCATTTGCCTCACAACATCGGCTGTGCATTTCGTTCTGGTGAATGCGATAGGAGCATCC GCAAGCACACATGTCTCAAACGAGCTAGGAGCTGGAAATCATAGGGCAGCTCGAGCCGCGGTTAATTCTGCCATTTTCCT TGGTGGTGTTGGTGCATTAATAACAACCATCACACTTTACAGTTACCGAAAAAGTTGGGGTTATGTATTCAGCAACGAGA GAGAAGTGGTTCGCTACGCAACCCAAATCACACCCATTCTCTGCCTATCCATTTTCGTTAACAGCTTTCTCGCCGTGCTC TCAGGGGTTGCCAGAGGATCAGGGTGGCAACGTATAGGAGGTTATGCAAGCTTAGGATCATATTATCTTGTCGGAATCCC GTTAGGATGGTTCTTGTGTTTTGTCATGAAATTGAGAGGGAAAGGACTTTGGATTGGTATACTCATAGCCTCCACTATCC AACTCATTGTTTTTGCTCTTGTCACCTTCTTCACCAATTGGGAACAAGAGGCAACGAAAGCTAGAGACAGAGTATTCGAG ATGACACCTCAAGTCAAAGGCAACCAAAAAACACAAATTATAGTAGAAGAGGATACACAAGTTTTACTTAATCATATTGC AGAAACTGTGTAAAAGTGTAAAACCACTTAATGCAACTCTATTGGTCTCCTTCGTTTTAGCATCTAATTACTACTCTTGG ATGCTTTACATTTGCATGATGTGTGATTTTAA .....N...........................................................i.............. .....................i..i....................................................... ..N....N..............N.....N..i.................i...........i.................. ..........................i.....i............................................... .............N.................................................................. ..............................................N................................. .......N.........i.............................................................. ................................................N............................... ................................................................................ .......................................................................N........ ..................N...................................N......................... ..........N..N....i........i...........................N........................ ...............................................................N................ ..........N..................................................................... ..............................................................N................. ................................................................................ ...........................................N.................................... ......N..................N...................................................... ................................................................................ i............................................................................... ..............................N................................................. N..............N..N............. Pos Score Pred ------------------------ 6 0.361 - 66 0.550 Yes 102 0.646 Yes 105 0.784 Yes 163 0.165 - 168 0.200 - 183 0.465 - 189 0.346 - 192 0.520 Yes 210 0.514 Yes 222 0.587 Yes 267 0.654 Yes 273 0.842 Yes 334 0.203 - 447 0.195 - 488 0.192 - 498 0.513 Yes 609 0.141 - 792 0.209 - 819 0.259 - 855 0.247 - 891 0.107 - 894 0.164 - 899 0.801 Yes 908 0.761 Yes 936 0.476 - 1024 0.224 - 1051 0.293 - 1183 0.411 - 1324 0.458 - 1367 0.233 - 1386 0.430 - 1521 0.562 Yes 1631 0.120 - 1681 0.048 - 1696 0.051 - 1699 0.054 -

Explain the output -- Processing locus 265: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCTAATGTTTGTGTGGAGGAGAAACAAGAGATCAAGATATGCTCAGATCAAGCAACTTAATCATCAATGAGGATTCTT AAAATTTTGATATTTTCTTGATTTGTAGAATGCTTCGTTTACGTAAGCAACAGTTTTGTTCGAATTTCGACTCATACTTA TATAGTAAACTGTAGCAAAAGTTCGGTTTATCAGAATCTTAAACCATTACGTATGGGAAAGTCCGGTTTATCACCAAACC AGTTTATGGAAACGAATTTGTATGATGTTCTAAATAACGATTTTAACCTCAAAGCTTCTGTTAAATTACGATTGTGCCAT AACTATATAAAGGAGAGACCCGAACAAACAATCCGGAAGCGGAAGCTAAAGTTAAGCAACGGCCGCCGCGAAGCTAACCT CCAAAGAAGGGGAAAAATGGCGAAAGGAGACGATAATGTGCAGAGGAAGAAGAATAAAGTGACGAGGAAGAAGATGAGTA GGAAGAACGATACCGCCACCGTATCTGCTCGTATCGCCGCCATTATCGCCGCCAAGAAACGTCGCAAGTCTGGCAAACGC AGCATGTGTCAGGGAATGTGTTTTACTCTTCCTACACTTGAAGATCCATTCAATGAAAGGCAAGGTAAAGCTGATATTAC TAAAAAGAAGAAGAAAAAGAAAAAGGTGAAGAGTAGAGAAGACAAGAAACCATCTCCTATGTCTATTGAAGGTGTTGAGA AGATGGATGGTCCACCAAAGTTTCTGATGTTAAACTTGAATGAGATTGAGAGCTCATTTCGTAAAGATATTACATACAGT GAACAACATGATAAGTCTTTGTTTACTAGTAGTTGGGGGATTGAGTTCTGGAAATGTTACTCATCTGGGAATGATATTTT GGACACGAGTGGAATGTCTTCCACTGTGGAACAGATTGCTTGGATTGTTTCTACTGCTGCTGATGCTATTGCTAGACGGG AGAAAGACGAGGTGGAGGAGGAGGAGGAGTTGTTGGGAAACAGTCCTTTTCTTTTGTACCTTGTGCCATCTCAGTCTAAA GCTTCTCAGGTTCGATCGGTTTGCAAGGCACTGAAAGGTATTGGAATTCATACTGTGAGCTTACACCAAGGAGCACCACT AGATCATCAGATTTCCGGATTAAAAAGCGTTGAGCCTGAATTTATAGTTGCTACACCTGAGAGACTTTTGGAGATTGTTA CCTTGAAGGGTGTTGATATATCCAACGTTTCATTGCTGGTCATCGATGAACTAGGTTCACTGTGCAGTGGTGGTTATCTA AATGCTGTTAAATCCATCAAGCAGGCCATTTCCAGCAAACACCAAACTATAGTTTTTAACAACAGTTTTAGTGCTTCCAT TATTCCGGCCGTACAGAGTTTCTTGGGGGGATCAGTTAACAGAGTTACTGTCAATGAGTCTGTTGCCAGCCAAGGCTCTT GTATTACCCAGACTGTTAGTGTATGCGCCTCGGAGGAAAAAAAACTGCAGAAGTTCGCAAAGCATCTGGATTCATCTTCG TCGAAGTTAATATACATTGTTACGAAGGAGGAAAGTTTCAAAAAGATAATGGCTATCCTTAAGCTCAAGGGTATCTCTGT ATCAACCAGTAGTGATTCAAAACTCTCAGAGGTCAAAAAGAGCCGGAAACCTGTGGCGCACTTGATTGATTTTGAACAGT TGGATACTACAGTTATGAGGGACTCTGAAACCGTTCTACTTCCAGACTTTTTCCCATCAATCGAAATTTACACTCAGATT CTGACAAGTATGGCTAGAGAGAGTGCTCACGGCGTACTTCACAGCTATATAACAGAAAAGGATGCTGCTTCTTATCAAGC TGGACCATTGGTGAATGTCCTGGAGAATTGTGGTCAGAACGTACCTGACCGTTGGAGGAATATGGATGTTGCTATGTCTG ATTGATGAAATGTTGTCTCCAATTCCATCTCTTTTAGTAACAATGGCTTTCAGAAGTTTTACCATCAGAGAATTTTGGTT AAAGCGATCATTTTGACTGTGTAT ......N.................................N...........................N........... .............................N.................................................. ....................................................N........................... .....N...............i..i....................................................... ................................................................................ ................i..................N.....................................i...... ................................................................................ ...i...........i....................................N........................... ..........................................................N..................... ..N...N...................N............N........................................ .......N.............................................N................N......... .............i................................................N................. ................................................................................ ................................................................................ ................................................................................ .............................................N.................................. .....................................................N.......................... ......................N......................................................... ................................................i............................... ................................................................................ ..............i................................................................. .........i...................................................N.................. ..............N..............................................N...N.......i...... ....N....N................................N..................................... ........................ Pos Score Pred ------------------------ 7 0.117 - 41 0.378 - 69 0.346 - 110 0.268 - 213 0.429 - 246 0.308 - 262 0.596 Yes 265 0.611 Yes 417 0.866 Yes 436 0.310 - 474 0.692 Yes 564 0.593 Yes 576 0.695 Yes 613 0.264 - 699 0.387 - 723 0.411 - 727 0.103 - 747 0.425 - 760 0.184 - 808 0.155 - 854 0.123 - 871 0.115 - 894 0.787 Yes 943 0.091 - 1246 0.170 - 1282 0.197 - 1414 0.295 - 1463 0.482 - 1569 0.577 Yes 1695 0.643 Yes 1770 0.612 Yes 1822 0.199 - 1855 0.307 - 1902 0.405 - 1906 0.124 - 1914 0.530 Yes 1925 0.106 - 1930 0.096 - 1963 0.041 -

Explain the output -- Processing locus 266: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCAATTATTCACATAAATGTTTTGTCAAATATTTAAGTGGACTTCATGAATTAAATTAGTGAAGTGAGCATTGCTCAAA GTTGTATATATTAAGCCATGAGCTTTCCTCATGACTCATAATATCTCAGTAAAACAAAATAACATACAAATGGATAGCAT TTGGATTCTATCACTTCTCTTCTTCATCATCTTTCTTCTTCTGGCCGCCTTCAAACGCAAAAATCATGGGAAACATCGAC GAATACCATCTCCTCCTGGTTTTCCAATCATCGGAAACTTACATCAGCTCGGAGAATTACAACATCAGTCTCTATGGAAG CTCTCAAAGAAGTATGGCCCTGTGATGCTTTTGAAGCTTGGAAAAGTCCCCACACTCATACTTTCTTCATCAGAAACAGC AAAACAAGCTCTAAGAGACTATGATCTCCATTGTTGTAGCCGTCCTTCCTTAGCAGGAGGAAGAGAGCTCTCTTACAACA ATCTAGATATGTCTTCATCTCCTTATAATGAATATTGGAAGGAACTAAGGAAGCTCTGCAGTCAAGAACTCTTTAGTGCT AATAAAATTCAATCGATTCAACCTATTAAGGACGAGGAGGTCAAGAAAGTTATCGATTCAATCGCGGAATCATCTTCTCT AAAGAATCCGGTTAACTTGAGCAAGACGTTTCTTGCTTTAACTACAAGTGTAGTATGCAAGGCAGCATTTGGTGTGAGTT TTGAGGGAAGTGTGCTCAACAGTGATAGATTCAATAAGTTAGTCCGAGATACTTTCGAGATGTTGGGAAGCTTCTCTGCC TCAGATTTTATTCCGTATGTCGGATGGATCATCGATAAGTTCAATGGTTTACAAGGGTGGAGAAAGAAAAGCTTTCGAGA TCTTGATGCGTTCTATGAACAAATTTTTGATCTGCATAAGGAAGAGAAGGAAGTAGGAAGTGAAGATTTAGTGGATGTGC TCTTGAGGTTGGAGAAAGAAGAAATTGTTGTTGGAAATGGCAAGCTCACAAGAAATCATATCAAAGCAATTTTGATGAAC ATTCTTTTAGGAGGAATCGATACTTCTGCAATAACAATGACATGGGCAATGGCAGAACTTGCTAAAAACCCTAGAGTGAT GAAGAAAGTTCAAGCAGAAATCAGAAACCAAATCAAGAACAAAGAAAGAATCAGCTTTGATGACACTGATAAGCTCGAGT ACTTGAAAATGGTGATCAAAGAAACATGGAGGTTACATCCTCCAACACCTCTTCTCCTCCCAAGAGATGTAATCACTGAA TTTGAGATCAATGGCTACACGATTCCTGCCAAAACACGGCTTCATGTGAATGTTTGGGCTATCGGGCGTGATCCTGATAC ATGGAAAGATCCAGAAATGTTTCTCCCCGAAAGGTTTAATGATAGTAACATTGATGCAAAAGGACAGAACTTTGAGTTGT TGTCGTTTGGGAGTGGTAGGAGAATTTGTCCTGGACTGTACATGGGAACAACAATGGTGGAGTTTGGCCTAGCTAATATG TTGTATCATTTTGATTGGAAGTTACCAGAAGGCATGGTAGTCGAAGATATCGATATGGAAGAAGCTCCTGGACTTACTGT GAGCAAAAAAAGTGAGCTTGTACTTGTTCCAGTGAAGTATTTAGACCATTGATTCACGTGAATTAGTTCTTCATCGATTG TACTATGAATAAGAACGAAATGATAATGATAATTTTCAATGTAATATTTTTTTTCTGTTTTGTTTTTGCCT .................i............................N................................. .................N............N......................................i.......... .................................................................N.............. .........................................................................i...... .............N..........i....................................................... ....................N........................................................... ........N..................N.................................................... ................................................................................ ......................................................N......................... ...........................................................N.................... ................N......N...................N.................................... .....N........N...........................................................N..... ....................................N.....................................N..... ....................................i....N......i.............................N. ...........................................................N.................... ........i................i........................................N............. ..........N................................N.....i.............................. i...............i.....................N..............N.......................... .........................................i...........N.......................i.. .................................N....................N......................... ................................................................................ ....N..............N.....N............N................................ Pos Score Pred ------------------------ 18 0.544 Yes 47 0.302 - 98 0.205 - 111 0.164 - 150 0.734 Yes 226 0.483 - 314 0.577 Yes 334 0.272 - 345 0.706 Yes 421 0.409 - 489 0.454 - 508 0.233 - 695 0.331 - 780 0.497 - 817 0.400 - 824 0.316 - 844 0.320 - 886 0.157 - 895 0.092 - 955 0.183 - 997 0.194 - 1035 0.393 - 1077 0.579 Yes 1082 0.483 - 1089 0.594 Yes 1119 0.357 - 1180 0.377 - 1209 0.668 Yes 1226 0.596 Yes 1267 0.419 - 1291 0.435 - 1324 0.253 - 1330 0.596 Yes 1361 0.652 Yes 1377 0.679 Yes 1399 0.162 - 1414 0.302 - 1482 0.605 Yes 1494 0.497 - 1518 0.700 Yes 1554 0.484 - 1575 0.483 - 1685 0.072 - 1700 0.055 - 1706 0.081 - 1719 0.057 -

Explain the output -- Processing locus 267: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGATGTTGAATCTTTCTCAACTTTTTGTAGTGTTTAGATGAGTAATTTGCTATCTTCTGCTAGTGAATCTTCTTCAAACA TATAAAACGCTGCGTTTCTGAGATCGTAGTGAAAATACCAATAAAACGGATAGTGTCCTCTTATCCGGAAACTGTGAAGT GAATAAAATGGAGAACTTCTCTTCAACTTACCGCCATTGTTACAGCTCCTTGTTTCTTCATCACTCGCAGCGAAGATATC TAGGTAACCATTCATTTTCTTGTTCTTCTCTGGAAATCAAACATGAGTCGTTTGAATCTATACCATTCTCAATATCTCTG GAACCGTCGTCTCCAAGTTGGGATTTTTATCCGTTGAAGAAGCTAAATGGATGCAAAAATCTGGTTTCTGTTAAAGCTAC TCATGCTCGGATGATGAAGTTATTCGATCGATTCGAATTGGAGTTCATCGCTAAGAGCTTGATCACTCGTTACCTTGAAT TTCAAGAATTTGGATATGCTAGTACTGTGTTCTTCATGGGGTTCCCTCGGAATCAAGTTTCATGGAGGGATTTTTTGGAA AAGGCTGAGAATTTCGGAGTAGAAAAGTATAGGGTCTTAGAGGAATTTGTTCAGCTACAGAACAAAGGAGTAAATTTTGA TGAAGTAGTTCTCGCTATGGTTTTGAGAATTTGTGCTGTTCTAATGTATCGGTTTCTGGGTTTAACTATCCATGGTGGTT TGATCAAGAGAGGGCTTGATAATTCAGACACACGAGTGGTTTCTGCGTCGATGGGTTTTTATGGAAGATGTGTGAGTTTG GGTTTTGCTAACAAGTTATTCGATGAAATGCCTAAAAGAGATGATCTTGCATGGAACGAGATCGTAATGGTAAACTTGCG GAGTGGGAATTGGGAAAAGGCTGTGGAACTTTTCAGAGAGATGCAGTTTTCTGGTGCTAAAGCTTATGATAGCACAATGG TTAAGCTTTTGCAAGTTTGTAGCAATAAGGAAGGTTTTGCAGAAGGGAGACAGATTCATGGGTATGTTCTGAGACTCGGG CTTGAATCAAATGTGTCTATGTGTAACTCTTTGATTGTCATGTATTCGAGGAATGGCAAGCTTGAATTATCCAGGAAAGT TTTTAACTCGATGAAAGACCGGAATTTGTCTTCGTGGAACTCGATTTTATCGAGTTATACGAAACTTGGTTATGTAGATG ATGCTATCGGTCTCCTGGACGAGATGGAGATATGTGGTCTGAAGCCAGATATAGTGACATGGAACTCTCTTTTGTCAGGT TATGCTTCTAAAGGCTTATCCAAAGATGCCATTGCTGTATTGAAGAGAATGCAAATTGCTGGTCTGAAGCCAAGCACGAG CTCAATTAGTAGTCTTCTTCAAGCTGTAGCTGAACCGGGGCATCTTAAACTTGGGAAAGCGATCCACGGATACATATTAA GAAATCAGCTTTGGTACGATGTGTATGTAGAAACCACCTTGATCGACATGTATATTAAAACTGGCTACTTGCCTTACGCC CGGATGGTTTTTGATATGATGGATGCGAAGAACATTGTTGCTTGGAATTCACTCGTCTCGGGACTTTCGTACGCTTGTCT TCTAAAGGATGCTGAAGCTTTGATGATTAGAATGGAGAAGGAAGGGATCAAACCTGATGCTATTACATGGAACAGTCTTG CTTCTGGGTATGCGACTTTGGGGAAACCTGAGAAGGCATTAGATGTGATAGGGAAGATGAAGGAGAAAGGTGTGGCCCCT AACGTGGTTTCATGGACAGCCATTTTTTCAGGATGTTCCAAAAATGGAAACTTCAGGAATGCTCTCAAAGTTTTCATTAA AATGCAGGAAGAAGGTGTTGGTCCGAACGCAGCAACTATGTCCACCTTGCTTAAGATATTAGGTTGCTTAAGTCTACTAC ACAGTGGCAAAGAGGTTCACGGTTTCTGTTTGAGGAAAAATTTGATCTGCGATGCATATGTTGCAACTGCACTAGTAGAT ATGTATGGAAAATCAGGGGATCTGCAAAGTGCGATTGAGATTTTCTGGGGCATTAAGAACAAATCGCTAGCTTCTTGGAA CTGTATGCTCATGGGTTATGCCATGTTTGGGCGAGGCGAAGAAGGGATCGCGGCTTTCAGTGTGATGCTTGAAGCGGGCA TGGAACCAGATGCTATAACGTTTACCTCTGTCTTGTCTGTCTGTAAGAACTCAGGTCTTGTCCAAGAAGGGTGGAAATAC TTTGATCTTATGAGGTCTCGTTATGGTATAATCCCGACCATCGAACACTGCTCTTGCATGGTAGATCTGCTCGGGAGATC GGGTTATCTTGATGAAGCCTGGGACTTCATTCAAACAATGTCATTAAAACCCGATGCAACTATTTGGGGTGCTTTTCTTT CATCTTGTAAGATTCATAGAGACCTAGAACTCGCGGAAATCGCTTGGAAGAGGCTTCAAGTACTAGAACCACACAATTCA GCTAACTACATGATGATGATAAACTTGTACAGTAATTTGAACAGATGGGAAGATGTTGAACGTATACGGAACTTAATGAG GAATAACAGGGTGAGAGTCCAGGATCTGTGGAGCTGGATTCAGATAGATCAAACGGTTCACATCTTCTATGCGGAGGGAA AAACACATCCTGATGAAGGAGATATATACTTTGAGCTGTACAAATTAGTCTCTGAGATGAAGAAATCAGGTTATGTGCCA GACACAAGCTGTATACACCAAGACATAAGTGATTCAGAGAAAGAGAAGCTGCTTATGGGACATACAGAGAAGTTAGCAAT GACGTATGGCCTTATCAAGAAGAAAGGCTTAGCTCCTATAAGAGTGGTCAAAAACACGAATATATGTTCTGATAGCCACA CCGTAGCCAAATACATGTCGGTTTTGAGAAACCGTGAGATTGTTTTACAAGAAGGGGCAAGGGTTCATCATTTCAGAGAC GGAAAATGTTCCTGCAACGACTCTTGGTGATAATATAGATACTACCTAGCTCTGTTTTTTCTAACAATGGTGCGTAAATA CAGACACTGTCCAACAAACACCAGAAAACAAATTACATCAAAACTTTGA ..N..................................N.......................................... ................................................................................ .......i........................................................................ ..........................................i..................................... ..............................................N...N............................. ..N.......N..N.................................................................. ...............N...................N.........................N.................. ...............................................................................N ................i..........................i...........................N........ ..................................................i.........N......i............ ......................N....i............N.........i...............N............. ........................................N........................N..........i... .........................................................N.....N................ ..........N.......N....................N............i........................... ..........N............................................................N.....N.. N......................i.......i..........................i..................... ................................................................................ ..................N.....N......................i................................ ...N...........i..i...N......................................................... ........N.............N........i........................N.........i............. .........N................................N.............i....................... ...........N....................N..........N..............N..................... ...................................................N.....N...................... i...N........................................................................... ....N.....i......N....N.........................................N..............N .........N...................................................................... .........i............N..................................N...................... ...........N.........................N...............N.......................... ................................................................................ .........N..N..N............................i.......N......................N.... ....................................................................N........... ............N...........................................i...............N....... ......................................................N.......................i. .....N.........................................................N................ ..............i................................................................. .....N............................................................N............. ................................................. Pos Score Pred ------------------------ 3 0.238 - 38 0.349 - 168 0.547 Yes 283 0.604 Yes 367 0.431 - 371 0.240 - 403 0.092 - 411 0.168 - 414 0.391 - 496 0.360 - 516 0.492 - 542 0.403 - 640 0.114 - 657 0.594 Yes 684 0.504 Yes 712 0.239 - 771 0.541 Yes 781 0.213 - 788 0.551 Yes 823 0.220 - 828 0.507 Yes 841 0.134 - 851 0.502 Yes 867 0.445 - 921 0.413 - 946 0.240 - 957 0.585 Yes 1018 0.270 - 1024 0.194 - 1051 0.131 - 1059 0.201 - 1080 0.289 - 1093 0.539 Yes 1131 0.388 - 1192 0.253 - 1198 0.169 - 1201 0.255 - 1224 0.505 Yes 1232 0.577 Yes 1259 0.719 Yes 1282 0.110 - 1306 0.365 - 1329 0.683 Yes 1459 0.115 - 1465 0.154 - 1488 0.641 Yes 1524 0.381 - 1536 0.806 Yes 1539 0.791 Yes 1543 0.189 - 1609 0.362 - 1623 0.324 - 1632 0.869 Yes 1657 0.268 - 1667 0.652 Yes 1690 0.078 - 1723 0.416 - 1737 0.544 Yes 1772 0.274 - 1793 0.296 - 1804 0.317 - 1819 0.294 - 1842 0.486 - 1878 0.757 Yes 1972 0.109 - 1978 0.174 - 2001 0.549 Yes 2005 0.097 - 2085 0.311 - 2091 0.618 Yes 2098 0.087 - 2103 0.310 - 2145 0.167 - 2160 0.288 - 2170 0.192 - 2250 0.594 Yes 2263 0.272 - 2298 0.270 - 2332 0.293 - 2358 0.435 - 2374 0.308 - 2490 0.303 - 2493 0.192 - 2496 0.262 - 2525 0.716 Yes 2533 0.305 - 2556 0.471 - 2629 0.474 - 2653 0.248 - 2697 0.635 Yes 2713 0.271 - 2775 0.412 - 2799 0.565 Yes 2806 0.285 - 2864 0.464 - 2895 0.578 Yes 2966 0.413 - 3027 0.097 -

Explain the output -- Processing locus 268: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACACCTTTATACAAAACGATATCCGTGAAGCTCCCTGAAAATAGAGATAGCCACACAATAGACATCGAAGTTTTTAGTT GATTTCCTGTTGAGAGCTTGAGACCAAAACAATTTTAGTCAACGCATGTTTTTTTATTTTTATTTTACGGAAAAAAATGG AATAGTAAATGATGATTGTTTTTCTCTCTTCCGATTGAATCACGAGATTGACCAAAAAAGGAAAATGGAAGACCTCTGGT TCATCTTCTTCTCCTTCCTCACAGCTACTTTAATCTTCTTCATCACCAAGAAGTTCCTGTGGAGTCTAAATTCTAAATTA CCTCCAAGCCCTACGCCTCTCCCTATCATCGGCCATCTTCACCTCATCAAGAAGTATCCTTTACCACAAGCTCTACGTCA TCTCTCTTCAAACTACGGCCCGGTTCTGTTCCTCAAATTTGGATGCCGTAACGTTTTGACTCTCTCTTCTCCAGATTCCA TCGAAGAATGCTTCACCAATCACGACGTAACCCTCGCGAATCGTCCCAAGACCATCACCTCCGATCACTTCAGCTACGGC TATAAAAACTTTGGATTTGCTCCTTACGGTGACCTCTGGCGAACTCTCCGTCGTCTCTCCACTCTCGAGGTCTTCTCCTC CGCTAGCCTGCAGAAGAACTCTTCCATCCGAAACGAGGAAGTCTCCAACCTCTGTTTGATTATCTTCAGATTATCCCGTG ACTCTCGGATAGTGGATCTCAAGTATCAATTCACTCTTCTCACGGCTCATATCATGCTTAGATTAGTATCTGGGAAGCGT GGGGTCAAAAAGAGCGATCCCGAATCAGAGAAGAGGTTCTTGGATGATTTTAAATTGAGGTTTTTCTCGAGCATGTCGAT GAATGTCTGCGACTATTTTCCAGTGTTGAGGTGGATTGGGTACAAAGGTTTGGAGAAGAGAGTGATTGATATGCAGAGAA TGAGAGATGAGTATCTTCAGCGACTCATTGATGATATTCGAATGAAGAACATTGATTCCTCTGGTTCCGTAGTAGAGAAA TTCTTGAAGCTTCAAGAATCAGAACCTGAATTTTACGCAGATGATGTTATCAAAGGAATCATAGTGCTAATGTTCAATGG CGGAACGGATACATCACCTGTGGCAATGGAATGGGCAGTGTCACTTTTACTAAACCATCCAGATAAGCTTGAGAAGCTCA GAGAAGAAATCAAATCTAATGTTAAACACAAAGGGCTTATACAAGACTCGGATCTCTCGAGCTTACCTTATCTTCGATGT GTCATCTATGAGACGCTTAGGCTATACCCTGCAGCTCCACTCTTGCTTCCTCACTGCTCATCCAAGAGATTCAACTTAGG CAACTACGAGATTCCAGAAAACATAATGTTATTGGTGAATGCTTGGGCTGTTCATAGGGACGGTGAGCTTTGGGAAGAAG CCAATGTTTTCAAGCCGGAGAGATTTGAAGGGTTTGTTGGCGATAGAGATGGTTTTAGGTTTCTGCCATTTGGAGTTGGG AGGCGGGCGTGTCCTGCAGCTGGACTTGGAATGAGGGTAGTGTCGCTTGCTGTTGGAGCATTGGTTCAGTGTTTTGAGTG GGAGAAGGTAGAAGCAGGAGATATAGACATGAGGCCCGTGTTTGGTGTGGCAATGGCTAAGGCTGAGCCGCTTGTCGCAT TGCCGAAGCCATGGTCTGAAATGGTTCCTATCTTGTCTCAGCTCTAGTGTCATCTGTGTTAGCCAAGAAATTTGATTTAG AGGATGCTTTTTATGTATACACATCAATAATAAGGAGGGCGAATGATCATAAATCACAAAAAATTA ................................................................................ .............................................N..............................i... ........N..N....................................................i............... ................................................................................ ................................................................................ ..........................................N..................................... .......N........................................................................ ................................................................................ ................................................................................ .....................................................N.......................... ...........................................N............................N.....N. ..N...................................................................N........N ......N.......................N..........i...................................... ........................................N..N.........................N......i... .........................i....N................................................. ..................i.........................................................N... .......N........................................................................ .........................i............N......................................... ...N............................................N............................... ..............................i................................................. ............................N.......................N........................... ..........N.........N........................................................... ...N........N.............................N....................... Pos Score Pred ------------------------ 126 0.351 - 157 0.687 Yes 169 0.375 - 172 0.468 - 225 0.582 Yes 443 0.376 - 488 0.377 - 774 0.437 - 844 0.125 - 873 0.398 - 879 0.351 - 883 0.463 - 951 0.451 - 960 0.272 - 967 0.172 - 991 0.247 - 1002 0.579 Yes 1081 0.214 - 1084 0.130 - 1110 0.446 - 1117 0.520 Yes 1146 0.617 Yes 1151 0.370 - 1219 0.635 Yes 1277 0.344 - 1288 0.248 - 1386 0.665 Yes 1399 0.363 - 1444 0.246 - 1489 0.178 - 1551 0.587 Yes 1629 0.307 - 1653 0.266 - 1691 0.488 - 1701 0.214 - 1764 0.051 - 1773 0.042 - 1803 0.046 -

Explain the output -- Processing locus 269: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAATTTTTTAAAGTAGCCCATAATTTATTGGTCTTACTATTTTAAATTTTTTAAAGTAGCCCATAATTTATTTACTAT TTTTAAACTAAATGATAGTAAAAAGTTATAAAAGGAATCCTTAAACCAGCGATGCTTCCTTTCACGTTGCAAACAAAACG AAGAGAACCATTCTCGTCCCTATATATACCATTTGCTCCTCTTCTTCTAATTTCACATATTATTCAACTACAAAACAAAA ACATTGCACATTTTTCCCTTTACTTTTCTTTAGCTCATTTCAAGAAGATGGTTTTATCTAAGACAGCTTCCAAATCCGAT GATTCAATCCATTCAACTTTTGCTTCCCGTTATGTCCGCAACTCTATCTCACGATTCGAAATACCTAAGAACTCGATCCC TAAGGAAGCAGCATACCAAATCATCAACGACGAGCTCAAGTTTGACGGTAACCCGAGGCTAAACCTGGCCTCCTTTGTGA CCACTTGGATGGAGCCAGAATGTGACAAGCTCATGATGGAATCCATCAACAAGAACAACGTTGAGATGGACCAATACCCT GTTACCACCGACCTTCAGAATCGATGCGTTAACATGATTGCGCGTCTCTTCAACGCGCCTTTAGGTGACGGTGAAGCCGC CATTGGTGTTGGCACGGTGGGGTCATCGGAGGCAGTGATGTTGGCCGGACTGGCCTTTAAGAGACAGTGGCAGAACAAGC GTAAGGCCCTAGGGCTGCCTTATGATAGACCTAATATTGTAACCGGAGCCAATATTCAGGTTTGCTTGGAGAAATTTGCA AGGTATTTTGAAGTGGAGCTTAAGGAAGTGAAGCTGAGAGAAGGATATTACGTGATGGACCCTGACAAAGCGGTTGAAAT GGTAGACGAAAACACTATATGCGTCGTGGCCATCCTCGGTTCGACACTAACCGGAGAATTCGAAGACGTTAAGCTCCTCA ACGACCTTTTAGTCGAGAAAAACAAGAAAACCGGATGGGATACGCCGATTCACGTGGACGCAGCGAGTGGTGGGTTTATT GCTCCCTTCTTGTATCCGGACTTGGAGTGGGATTTCCGGTTACCGTTGGTTAAGAGCATAAATGTGAGTGGTCACAAATA CGGTTTGGTTTACGCCGGTATCGGTTGGGTCGTATGGAGAACCAAAACCGATTTGCCTGATGAACTTATCTTCCATATCA ATTATCTTGGAGCTGATCAACCCACATTTACCCTCAACTTCTCTAAAGGGTCAAGTCAAGTGATTGCTCAGTACTACCAG TTGATTCGTCTTGGATTCGAGGGATATCGCAACGTGATGGATAATTGCCGCGAGAACATGATGGTACTAAGACAAGGATT AGAGAAAACGGGACGTTTTAACATCGTCTCCAAAGAAAACGGTGTTCCGTTAGTGGCGTTTTCTCTCAAAGATAGTAGCC GCCACAACGAGTTCGAGGTGGCCGAAATGCTTCGTCGCTTCGGCTGGATCGTTCCGGCCTACACGATGCCTGCGGATGCG CAACATGTCACGGTCCTTCGAGTTGTTATCCGAGAAGATTTCTCTCGAACCTTAGCTGAGAGATTGGTAGCCGATTTCGA GAAGGTTCTACACGAGCTCGATACGCTTCCCGCGAGGGTTCACGCCAAGATGGCTAGTGGAAAAGTTAACGGTGTTAAGA AGACGCCAGAGGAGACGCAAAGAGAAGTCACGGCCTACTGGAAGAAGTTTGTGGACACTAAGACTGACAAGAACGGCGTT CCGTTAGTAGCAAGTATTACCAATCAATGATGGGCCAAATGTATATTCAAATTATTGGGTGCAGACAAATTTTTTTTTAG CTAAAGGCCCAACTACACCATAGCAAATCAGCACCATTCGATCTCAGAC ................................................................................ ...........N.......................................N............................ ................................................................................ ...............................................i..............................N. ...............................N................................................ ................................................................................ ........i..........N............N..i.............................i.............. .......................N.........i.............................................. .....................................i.......................................... .....................N.......................................................... ......................................................i.......................i. ..................i............................................................. ..................................i............................................. .............................................................i.................. .................................N.........................N.................... ................................................................................ ....................................i....................i..N................... ................................................................................ ..........................i......................................i.........N.... ....N........................................................................... .................................................i.............................. ................................................................................ ..........................N..N........N......................................... ................................................. Pos Score Pred ------------------------ 92 0.245 - 132 0.153 - 288 0.766 Yes 319 0.451 - 352 0.174 - 489 0.570 Yes 500 0.440 - 513 0.407 - 516 0.731 Yes 546 0.764 Yes 584 0.466 - 594 0.902 Yes 678 0.573 Yes 742 0.285 - 855 0.737 Yes 879 0.815 Yes 899 0.552 Yes 995 0.769 Yes 1102 0.619 Yes 1154 0.271 - 1180 0.160 - 1317 0.553 Yes 1338 0.525 Yes 1341 0.348 - 1467 0.638 Yes 1506 0.671 Yes 1516 0.189 - 1525 0.240 - 1650 0.786 Yes 1787 0.452 - 1790 0.311 - 1799 0.173 -

Explain the output -- Processing locus 270: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTCTTCGTGTGTGTTGTCTGGGATTGAGAGTCTTCGTTTTGTTGTTTGTTATTTAGACAGTTACTGTTTCGAAATAATG AACTAAACGGATCTGTCTTAAGAATTGTGTTTCTTTGGTAGACTTGTTATGTTGTCTGATTCTGATTTCACTTTAGCTCT TCTGCTACAGGATTATATAATAGGACAAAGAGAGAGAGACAACATGCACTCTCTTAAGACTACTTGCGTTGGGCAAATAT TTGCTCTAGCTAAGCCTCACGATTCTGTAGGCAAGAGGACTCGTAATCGGATTCCTAAAGAAGAGAGAAAGACACTGGTT GAATCTTTTATAAAGAAGCATCAGAAACTAAACAATGGGAGTTTTCCTTCACTTAGCCTCACACACAAGGAGGTTGGTGG ATCTTTCTACACTATAAGGGAGATTGTTAGAGAGATTATCCAAGAAAATAGAGTCCTTGGTCCTGGTGACTTGCTACTTG AGGGAAATGGCTCTGTGCAAGATCAAAGTCTGTCAAGTTCAATCCTGATGGATCCTGTGCCCCCTTTATCTTTGTCCCCA AACGGATTTCATTCTGGGTCATATCAAAGTCTTGATTTTTCTTCTGAGTCACCTGAGGGAAACGTTAACGGTAGCCAGGT TTGCTTGGATAATTGCAGGGAAGTGAGTGGAAGTCAACTTTTGAAAGAAGACATTGGGCTCGTACACCAGTCAATGGACT CCACTGACATATCTATGACTCAGCTTGCAACATCTTGCAGTGAAGACAATGATATCAAGAGTAATGCAGGGCTACAAAAT AGAATGGAGACAGTGTGCGATAGCGTTGACACCAAACCACAGGATAAAAGACTCGATGTGGATAACAAAGACGAAGGATT TGAGGAACTTCGTTTCATGGAATCAGACGGCACAAAGCCTGTTAACAATGACGATAGAGTGAATGATGCAGGAGCAGCAA TGACCGAAATTAAGAACGGATTAGGCACAATTGATATGTCAGCAGAAACAGTTGTTGAGACGTTTCCATTAAAATCTGTG ACTTCAACGATGGACTCACCGGATGCACAACCTACTGAGTTGAACAAAGTTTGTGAGGGTGGGAAAGGAACTGAAACGGA GGTGGAAGCTGATCGCAGCACAGTAAATCATGTTGATCTTGGGGAGATTTCATCATCCACTTCATCTGCTGTACTTGAAG ACATAGGGACGGAAGTCATTGTTGGTCAAATACCAAATCATATTTCTGTCCCTATGGAAAAGAAAGTTGGAGAGGAAATT GTAAATTCTGCTTCAGTAGATGTTGAATGTGCTGATGCTAAAGAGACAGTTGTTGTGAATGGTGTGATTGGCAACGTCCA TGAGACAAAGGAATTTAGTAATGGAACTTTGACAGCTGAACAGAAAATGCCAACATCTAGCACTGAGTCGGGAAGTCGTA AGAACGACAGAGCAAAAGTGGACACTGTGAGTAGCTATGCCGGGAATGAAGTCGCAAGTGTAGAGAAGAAGGCAACCATG GAAAAAGGGAAAATTGATGCTCCAGATAGTTCCAGCTCTCAAAAAGAAAACAATGCAACATTAAACAGAATTAAACCTGA ATCGTGGAAAGGGGAATCTAATATGGGAAGACAAGAAACAAATCCTCTCTTGGCAGTTCTGAAATCTTTCGTGACAGCCT TTGTGAAGTTTTGGTCAGAGTAATAATTCATTGTAGAGATCCGTTGGTTAAACTTTTGTAGTTTTCATTAGTGAGAGTTG GTAAGCTTAATATGGTTTGAAATGTGCTGTACAATTTTTGTG .............................................................................N.. ................................................i............................... ...........................................i.................................... ................................................................................ ..................................i............................................. ................................................................................ ......N........................................N................................ ................................................................................ .........................................................................i...... ..............i.................................N..............N................ ...i...................................................N........................ ................i..............................N..............N..N.............i ...................................i............................................ .........i............N......................................................... .............................N.................................................. .....................................................i.......................... ...................N......N.......N.......................N....................N ....................N.........................i................................. ....................................N........N...............................i.. ................N...................................N........................... ......................i......................................................... ................................................................................ ...........N.........N.................... Pos Score Pred ------------------------ 78 0.228 - 129 0.533 Yes 204 0.684 Yes 355 0.638 Yes 487 0.328 - 528 0.403 - 714 0.795 Yes 735 0.585 Yes 769 0.307 - 784 0.323 - 804 0.893 Yes 856 0.255 - 897 0.802 Yes 928 0.227 - 943 0.076 - 946 0.133 - 960 0.793 Yes 996 0.706 Yes 1050 0.800 Yes 1063 0.276 - 1150 0.170 - 1254 0.822 Yes 1300 0.115 - 1307 0.307 - 1315 0.124 - 1339 0.128 - 1360 0.187 - 1381 0.452 - 1407 0.807 Yes 1477 0.364 - 1486 0.193 - 1518 0.596 Yes 1537 0.317 - 1573 0.201 - 1623 0.695 Yes 1772 0.058 - 1782 0.074 -

Explain the output -- Processing locus 271: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAACATGTTTTGCCAGCTTGCTAGGTCTCTCTATAAATATTGGTTCCTTCTCTTAACATCATTTTTTCTTCTCTCGAGT GTTGTTAAAGTTTTTGTATAGTTTGCTATTTTTCGTGAATCAATAAAAAGAGCTTTGTTGAACAAATTAGCTATCTCCAT GGACCGACCAAAGAAACGGGACGTACTACGAGCGGTGAATTCGATGAGCTCTCTATGCGAGAATGGCAAAAAAAAGGAAG GAATTCGAGCAGTAAACTCGATGTGCTCGATATCCGAACTGACGGATGAAGACCTACTGTCTCGGCTGCTTGACCGTCCA AGACTCAAACTTGAACGGAAAATATCATACGAGGAAATGTCTTTAAGCCTTTACGACAGTGCACATTCACTAGACGGTAA GTCTGGTTGGGATACTCCGGTTTTCTCCATGAAAGACTCAATGGATCGTAACCCGATGGTCACAGAAGCTTGGGAGGCTC TCTGCCAGTCTCAGGTCTACTTCCGGGGAAAACCGGTAGGAACCATCGCCGCCTACGACCATGCTTCAGAAGAGGTCTTG AACTATGATCAGGTGTTCGTACGTGATTTTGTACCGAGTGCTCTAGCATTTCTGATGAATGGAGAGCCTGAAATTGTGAA GAACTTTCTCCTGAAGACACTTCATATTCAGGGACAAGACAAAATGATCGACAAGTTCAAGCTTGGTGACGGCGCAATGC CAGCAAGCTTTAAAGTTCTACATAATCCTATCAAGAAGACGGATACGATTATCGCTGACTTTGGAGAAAGCGCCATCGGG AGAGTGGCTCCAGTCGACTCTGGCTTCTGGTGGATCATTCTCCTCCGAGCTTACACTAAGTCTACAGGAGATCATTCTCT AGCAGAGAGACCAGAGTGCCAAAAGGGAATGAGACTCATACTTTCTTTGTGTCTCTCTGAGGGTTTTGATACTTTTCCGA CTTTGCTTTGCGCAGATGGTTGCTCGATGGTCGATCGTAGAATGGGCATTTACGGCTATCCGATTGAGATCCAAGCCCTC TTTTTCATGGCTCTTCGATCCGCACTCTCAATGCTGAAACATGATTCTGAGGGTAAAGAGTTCATGGAAAAGATAGTGAA GCGGCTGCACGCACTAAGCTTCCATATGAGAAGCTACTTTTGGCTCGATTTCCAGCAGCTCAATGACATTTACCGTTACA AGACGGAGGAGTACTCTCACACCGCGGTAAACAAGTTCAACGTAATCCCTGACTCCATCCCTGATTGGATCTTTGACTTC ATGCCTCTCCGAGGTGGCTACTTCGTTGGAAATGTCAGTCCCGCCCGCATGGACTTCAGGTGGTTTGCGTTAGGAAATTG TATCGCAATTCTTTCCTCATTGGCCACACCAGAGCAGTCCATGGCGATCATGGACCTGATTGAGGCCCGGTGGGAAGAAC TTGTGGGAGAGATGCCGTTGAAGATATGTTATCCGGCGATGGAGAGTCACGAGTGGGGGATTGTCACTGGCTGTGACCCT AAGAACACAAGGTGGAGCTACCACAACGGAGGCTCTTGGCCAGTGCTTCTATGGTTACTAACGGCGGCAAGCATAAAGAC TGGACGGCCACAAATAGCACGACGGGCTATAGAGTTGGCGGAGGCACGACTGTTGAAGGATGGATGGCCGGAGTACTACG ACGGAAAGTCTGGAAGATTCATCGGAAAACAGGCTAGGAAATCTCAGACATGGTCAATCGCTGGCTATTTGGTCGCCAAG ATGATGATGGATGATCCAACGCATGTGGGAATGATCTCAATGGAAGAAGAGAAACATATGAAACCTCCTCTTAGAAGATC TTCTTCTTGGACTTAAATTATATAATTTCGTTTTGTGTTTTTTTTTCTTCATTGTAGGGCCTTTTATAATTACCATTAAC AGAAACAAAAATATTCATAGCTCCCAAATTAACTT .....N.......................................................................... ..............................................................................N. ...........................................N..........i.......N................. ....................N........................N.................................. ....................................i........................................... ............................i...........i..............i........................ ............................................................N................... ....N.................................................i...N..................... ...........................................i................................i... ................................................................................ ................................................................................ ............................i................................................... ...............N..........i..............i...................................... ......N.......................N.........N......................N................ .........................N....................................N................. ................................................................................ N..............................N................N............................... ........................................i........i.............................. ...........i.............i............N......................................... ..................................................i............................. ...........................................................i...i................ .................................................i.............................. N..i..N...N...........N.......N........N.................N...................... ................................................................................ ................................... Pos Score Pred ------------------------ 6 0.344 - 159 0.498 - 204 0.195 - 215 0.515 Yes 223 0.432 - 261 0.229 - 286 0.402 - 357 0.713 Yes 429 0.586 Yes 441 0.765 Yes 456 0.770 Yes 541 0.181 - 565 0.139 - 615 0.787 Yes 619 0.298 - 684 0.640 Yes 717 0.821 Yes 909 0.622 Yes 976 0.243 - 987 0.510 Yes 1002 0.699 Yes 1047 0.304 - 1071 0.163 - 1081 0.430 - 1104 0.446 - 1146 0.444 - 1183 0.201 - 1281 0.185 - 1312 0.466 - 1329 0.365 - 1401 0.576 Yes 1410 0.508 Yes 1452 0.501 Yes 1466 0.590 Yes 1479 0.233 - 1571 0.755 Yes 1660 0.545 Yes 1664 0.597 Yes 1730 0.745 Yes 1761 0.315 - 1764 0.620 Yes 1767 0.321 - 1771 0.147 - 1783 0.114 - 1791 0.107 - 1800 0.209 - 1818 0.140 -

Explain the output -- Processing locus 272: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGAAAACCGTTTTCACAACTTTTTTTTATAAAAAAAAAAATCTGCAAAAAGATCTCTCTGATAAGAATTTTTAAAAACT TTCCCTTTATGGATGCTCTGAAACATAAAAGAGCTTTTAAGTTTATACCTTCCCTTATATTCTCTGTTTTCAAATCTGTC AGAATCTGATCACTCTTTTCTTTTTATCTACTTCAGGATGTTGGAGAAGGTTGAAACTTTCGACATGAACAGAGTCATTG ATGAATTCGATGAAATGACTAGAAACGCTCATCAAGTCCAGAAACAAACTTTGAAAGAGATTCTTCTCAAGAACCAATCC GCCATTTACTTACAAAACTGTGGTCTCAATGGAAACGCTACTGACCCTGAAGAAGCTTTCAAATCTATGGTTCCTTTAGT CACTGATGTTGAATTAGAGCCTTACATCAAAAGAATGGTTGATGGTGACACTTCACCTATTCTCACTGGTCACCCTGTTC CTGCCATTTCCTTAAGCTCTGGAACTAGTCAAGGCCGTCCAAAGTTTATTCCTTTCACTGATGAATTAATGGAGAACACA TTGCAACTGTTTCGCACTGCTTTTGCCTTCAGAAACAGAGATTTTCCCATTGATGACAATGGCAAAGCTTTGCAATTTAT CTTCAGCAGCAAGCAGTACATATCAACGGGAGGTGTCCCGGTTGGAACCGCAACCACAAACGTGTACCGCAACCCTAACT TCAAAGCTGGGATGAAATCTATAACGTCTCCTTCCTGTAGTCCCGACGAAGTTATCTTTAGTCCTGATGTCCATCAAGCC TTGTATTGCCATCTCTTATCCGGAATTCTCTTCAGAGATCAAGTTCAATACGTCTTTGCCGTCTTTGCTCACGGTCTAGT TCACGCTTTTAGAACCTTTGAACAGGTTTGGGAAGAGATTGTTACCGATATCAAAGATGGTGTCTTAAGCAACCGTATTA CTGTCCCATCGGTCCGTACTGCAATGTCGAAGCTGCTCACACCTAACCCTGAGCTAGCTGAGACGATCCGCACCAAATGT ATGAGTTTGAGCAACTGGTACGGATTGATTCCCGCGCTCTTCCCCAACGCAAAGTATGTTTACGGGATCATGACTGGCTC CATGGAGCCGTATGTGCCAAAGCTGAGACATTACGCGGGTGATCTACCTCTTGTGAGCCATGACTACGGTAGCTCGGAAG GATGGATTGCTGCTAATGTTACCCCAAGGTTATCTCCAGAGGAAGCTACATTTGCTGTGATTCCGAATCTCGGTTATTTC GAGTTTCTCCCTGTATCTGAAACAGGGGAAGGAGAGGAGAAACCGGTTGGTTTAACTCAAGTCAAGATTGGAGAAGAGTA TGAAGTTGTCATCACAAATTACGCAGGGTTGTATCGATACCGGCTTGGAGATGTGGTAAAGGTCATTGGTTTCTACAACA ACACTCCCCAACTAAAATTCATATGCAGGAGAAACCTGATTCTCTCGATCAACATCGACAAGAACACCGAAAGAGACCTT CAGCTTTCAGTAGAATCGGCTGCAAAGAGACTCTCAGAAGAAAAGATCGAGGTCATAGACTTCTCTAGCTACATCGATGT CTCGACAGATCCAGGACATTACGCTATCTTCTGGGAGATTTCAGGAGAAACAAACGAAGACGTTCTTCAAGATTGCTGCA ACTGTTTAGACCGAGCATTCATCGATGCGGGTTACGTTAGTTCCCGGAAATGCAAGACTATTGGAGCCTTGGAGCTGAGA GTTGTCGCAAAAGGAACTTTCCGGAAGATTCAAGAACATTTTCTCGGGCTTGGTTCATCGGCTGGACAGTTTAAGATGCC AAGATGTGTGAAGCCAAGTAATGCTAAGGTTCTCCAAATTCTGTGTGAGAATGTGGTGAGTAGCTACTTCAGCACAGCGT TTTGACTCACTAAAATTGTGTGAAATCAAAGAAAGTGGTTTTTAGTTGTTTATTTCCGTCGTTGTTGTCTTTATTTGTCA ATATAGTTTTCTTTACGTTATTTA ................................................................................ ........N...N................................................................... .....................................i..........................i............... N........N....i................................................................. ............................N.....................................i............. .....N............................i......N...................................... ............................................................N.......i........... ....................................................N.....i..................... ................................................................................ ...........N......................................................i............. ................................................................................ ........................................................N....................... .......................N....................................................i... N......................................................N.............i.......... ...............................................................................N ..................................................N............................. ......................i......................................................... ............................................................................i... ................................................................................ ........................N........................N.............................. ...........................................................................N.... ...i................N.............................N............................. ................................................................................ ........................ Pos Score Pred ------------------------ 89 0.260 - 93 0.124 - 198 0.742 Yes 225 0.667 Yes 241 0.122 - 250 0.305 - 255 0.559 Yes 349 0.208 - 387 0.840 Yes 406 0.180 - 435 0.877 Yes 442 0.227 - 541 0.082 - 549 0.858 Yes 613 0.341 - 619 0.505 Yes 732 0.234 - 787 0.581 Yes 937 0.468 - 984 0.480 - 1037 0.654 Yes 1041 0.491 - 1096 0.467 - 1110 0.795 Yes 1122 0.512 Yes 1132 0.182 - 1180 0.477 - 1202 0.288 - 1216 0.349 - 1360 0.173 - 1411 0.234 - 1463 0.662 Yes 1597 0.572 Yes 1705 0.397 - 1730 0.390 - 1836 0.439 - 1844 0.684 Yes 1861 0.376 - 1891 0.230 -

Explain the output -- Processing locus 273: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCGACTTTGACGTCTTTGAGCATCCATCCATGTTGAAGAATCCCATTATTCGTATGCTTTTCTTCTATGGCCTTGCATCA GTCAGCACTCAACTTGTTTGAATGTGCAGGAGCGTGTTTCCAGAGGGGATGGATTCTGTTCGACGCACTTACACCATTAT GCGAGGTCGTCACATAGACAAGCGTGTTCCTTCTAAAGAGTTTCTCTCTGCTTTGGATAAAGCTGAAACTGCAGTACGTC TTCCTACTGGGACGGGAAAAGACTGCGGGAATCCTTCCTGGAAGCGATCTCTGCCTCACGTGCTCGTTGCTTCTCTGACT TCGCTTTTATTCGGCTATCATCTCGGGGTTGTTAATGAAACTCTCGAAAGCATATCAATTGACCTTGGCTTTAGTGGGAA CACCATAGCTGAAGGTCTTGTGGTTAGCACCTGCTTAGGCGGCGCCTTTATTGGGTCTCTGTTCAGTGGGTTGGTAGCTG ATGGTGTTGGGCGCCGTAGAGCTTTCCAGTTGAGCGCTTTACCAATGATTGTTGGAGCTTCTGTGAGTGCATCAACTGAG AGTCTTATGGGCATGCTCCTGGGAAGGTTTTTGGTCGGAATTGGGATGGGCATAGGTCCTTCTGTTACAGCTCTCTATGT CACTGAGGTGTCACCTGCTTATGTGAGAGGTACATATGGGAGTTCTACCCAAATCGCAACATGTATTGGACTCTTAGGTT CGCTGTTTGCTGGAATCCCGGCAAAAGATAACTTGGGCTGGTGGCGCATATGTTTTTGGATATCCACAGTCCCAGCTGCA ATGCTTGCTGTCTTCATGGAACTCTGCGTGGAAAGTCCTCAGTGGCTTTTTAAGAGGGGAAGGGCAGCTGAAGCTGAAGC TGTGTTTGAGAAGCTATTGGGAGGGTCGTACGTCAAAGCTGCTATGGCAGAGTTAGTGAAGTCGGATAGGGGAGATGACG CCGATTCAGCCAAATTGTCGGAGTTGCTTTTTGGACGCAGTTTTAGAGTGGTTTTCATTGGATCTACCCTTTTTGCTTTA CAACAGTTGTCTGGGATAAATGCTGTGTTCTATTTCTCATCAACTGTCTTCAAGAAAGCTGGTGTACCTTCAGCCTCTGC AAACATATGTGTAGGAGTTTGCAACCTCTTAGGATCAACTGTTGCTGTGGTTTTGATGGATAAGCTGGGGAGAAAAGTGT TGCTTATTGGGAGTTTTGCGGGCATGGCAGTATCGCTGGGTCTCCAAGCAATAGCATATACTTCGCTTCCTTCGCCCTTT GGAACCTTGTTTCTCTCGGTTGGAGGAATGCTGCTGTTTGTGTTGTCGTTTGCAACTGGAGCGGGTCCTGTTCCAAGTCT TCTCCTGTCTGAGATATGCCCTGGTCGACTTCGAGCAACTGCGTTGGCTGTTTGTCTTGCCGTTCACTGGGTTATAAACT TCTTTGTCGGGCTGCTGTTTCTGAGAATGCTGGAGCAACTAGGTTCGGTGCTTCTAAATGCAATATTTGGGTTCTTCTGT GTGGTGGCAGTCATCTTTGTGCAGAAGAACGTGGTTGAGACTAAAGGAAAGAGCCTACAAGAGATAGAAATCAGTCTGCT CTCTTCCACCCAGTGATTGATCTCTTTATTCTGAGAATTCGTATTATTCTTCTGGCTCTCTTTTTTTCCTTGGAGAGACA CAAGTAACGTGTACCTAATATGGAAGTAGGAGTGA .............................N.......................N............i............. .....................i..........................i.............................N. ................................................................................ ................................................................................ ..................................N............................................. ................................................................................ N...........................................N................................... ......i.....i................................i..............................N... ....................N..............N........................i................... .................................................N.............................. i..............i................................................................ ...........................................N..............................N..... ................................................................................ ...................N............................................................ ......N................................................N........................ .......................N........................................................ ...........................i.................................................... ...............N................................................................ ..........................i..............................i...................... ................................................................................ ................................................................................ ...................N............... Pos Score Pred ------------------------ 30 0.456 - 54 0.236 - 67 0.586 Yes 102 0.530 Yes 129 0.688 Yes 159 0.495 - 355 0.230 - 481 0.232 - 525 0.384 - 567 0.637 Yes 573 0.563 Yes 606 0.601 Yes 637 0.295 - 661 0.247 - 676 0.334 - 701 0.760 Yes 770 0.444 - 801 0.592 Yes 816 0.502 Yes 924 0.391 - 955 0.185 - 1060 0.442 - 1127 0.385 - 1176 0.414 - 1224 0.145 - 1308 0.547 Yes 1376 0.384 - 1467 0.511 Yes 1498 0.534 Yes 1700 0.067 -

Explain the output -- Processing locus 274: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 275: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTAATTTTTTGCCTTTTTGGTAAGGAAAGTAACATAGTTGTTAGTAAATATGAATTAGGAAATAATATACAAAGTCTTGG TCCTCTTCTCATCGACTTTAAAATCTCTTTCTCCCATTACAAACCAAAACCATTAGAAGATTTCGAAAAAGAAAAAAAAA CACCTCCACAAACAAAACAATGGCGGTGAAGAAGAAATCATCTTGGAGATCATTGATGGTTTTGTGTTTCCAAGATCCAG ACAACATATATTCTCCCAAGAAGACAAAGAAAGATGATGGTGAACGTGTGATTACCAAACAGAAATCGTTTCTTGGATTG TCGATTCTAGACATAAGTAATCCAAGTTCCACTACCTTGTCCGAAGATCTCTCAATCTCCCTCGCCGGCTCGGATCTCCA CGTGTTCACACAAGCAGAGCTTAGAGTCATAACACAAAGCTTCTCTTCTAGCAACTTTCTTGGTGAAGGAGGGTTTGGTC CGGTTCATAAAGGGTTCATCGATGACAAGCTCCGTCCTGGTCTTAAGGCTCAGCCCGTTGCCGTTAAACTTCTTGATCTT GATGGTCTTCAAGGTCACCGTGAGTTTATGACGGAGGTTATGTGTTTGGGAAAACTAAAGCATCCGAATCTGGTGAAGCT GATCGGATATTGCTGCGAGGAAGCACATAGACTTCTTGTTTATGAGTTCATGCCTCGTGGAAGTTTGGAGTCTCAACTCT TCAGAAGGTGCTCCCTACCGCTGCCATGGACAACGAGGTTGAACATCGCTTATGAAGCTGCAAAGGGTCTACAGTTTCTA CATGAAGCCGAGAAGCCCATCATCTATCGCGATTTCAAGGCCTCTAATATCTTGTTAGATTCTGATTATACAGCGAAACT CTCGGACTTCGGGCTAGCAAAAGACGGACCACAAGGCGATGATACACATGTGTCGACTCGCGTGATGGGCACACAAGGGT ACGCGGCGCCAGAGTACATAATGACAGGTCATTTAACGGCTAAGAGTGATGTGTACAGCTTTGGAGTGGTGTTGCTTGAG CTGTTGACAGGTCGGAAATCGGTGGACATAGCGAGATCATCAAGAAAAGAAACCCTCGTTGAGTGGGCTCGTCCAATGCT CAACGACGCACGTAAACTCGGGAGAATAATGGACCCGAGGCTTGAAGATCAGTACTCAGAGACCGGAGCACGAAAGGCAG CAACTCTAGCTTATCAATGCCTAAGGTACCGACCAAAGACAAGGCCAGACATTAGCACTGTTGTCTCCGTTCTCCAAGAC ATCAAAGATTACAAAGATGATATTCCCATTGGGATATTCACTTACACCGTTCCTACCAAGCCACGCCGGGAAGTTAAAGA GACAAGTCTTCAAAACTTTGACAAGCCACGCCGTGAAACTAAAGTGACAAGTCTTCAAAACTTCGACAAAACACGCCGTG AAGTTAAAGATACAAGTCTTCAAAACTTCGACAAAACACGCCGTGAAGTTAAAGAGACAAGTCTTCAAAACTTCGACAAA ACACGCCGTGAAGTTAAAGAGACAAGTCTTCAAAACTTCGACAAGCCTCGCAACGTTTCCACGACTGATAACCATCAAAA GTTCCGGTCGCCCGCACACACGGCAAGAAACCACCGGATAACTTTGAGAAACGGATATAATTCACCAATGCGTAACGAGG CCGGAGGGGAACGGTACTGACTACTGAGTTCTCTTCTATCCTCAATTCGTAATCTTTTCATTTTTTTTACATAAGATGTA AATAGTAATACATTCTTACCACCAAAAAAACAAAAAAAA .................................................N.............................. ................................................................................ ...................i...................................N........................ .................................N..N........................................... ................................................................................ ................................................................................ .....................N.......................................................... .........................................N.......N.............................. .........................N.........................N............................ ......................................N........N................N............... ....................N...........................N............................... ...........................................................................i.... ............................i................................................... ................N............................................................... ................N............................................................... ................................................................................ ................................................................................ ................................................................................ ...................................................................N............ ...........................................................................N.... ....................................... Pos Score Pred ------------------------ 50 0.444 - 180 0.788 Yes 216 0.280 - 274 0.374 - 277 0.166 - 502 0.212 - 562 0.136 - 588 0.163 - 600 0.620 Yes 682 0.302 - 690 0.379 - 746 0.323 - 772 0.427 - 802 0.213 - 919 0.097 - 928 0.395 - 945 0.443 - 981 0.260 - 1009 0.218 - 1116 0.639 Yes 1149 0.577 Yes 1217 0.232 - 1297 0.408 - 1668 0.351 - 1756 0.042 -

Explain the output -- Processing locus 276: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTCGGAATTTTGTTGGGTATCTGATTGTTTTGTCGATTTGTTCGTTTTGACGTATAAACCCTAATTTCGAGTCTTGTGA TGTCGCGTAGATAAACCCTAATTTCAGTCGCTTTTTATGTTTGCTTACAGGTTTTTATTTGTTCTTGTAAGAATGGTTGC GATACCCAATATTAAGATAAAGTTTGGTCCTCAAGGTTCTGTCAGAACATTCCAAACGTTGTCTGATAGTTCGAAGAAAA TTGAGCATGTAGTGACTGAAGATTTATCTCAATCCTCGGAGAAATCAAAGAAGCGTGGTGGGCCTAAAGAGCTAGATGAA GTTCAACCTAAGAAGAAGCAGAGGCTGGATTGTGATTGGTCTAGTCAATGTTTGGCATTGTTACGTTTTTTGATGGAGCA CCGTGGTGGATGGCTCTTTAAGGAGCCTGTTGATCCTGTTAAAATGGAGATTCCTGATTACTTCAATGTCATACAAAAGC CAATGGATTTAGGCACTGTCAAATCGAAACTTTTGAAGAATGTCTACAGTAATGCTGATGAGTTTGCAGCGGATGTTCGG TTAACTTTTGCCAATGCGATGCATTATAATCCTCTGTGGAATGAGGTTCACACGATTGCAAAGGAGATTAATGAGATTTT TGAGGTTAGATGGGAATCACTCATGAAGAAAAAGGTTTTGAGGTTATCCTGGAATGAAGTTAGAGAAGGTTATAAGAGAC AGCCAGTTGAACGTGATTGTTCTAGACGGAGCAGCACTGGAACATCAGCTTCCTCTGGGGTGGGATTGACGAAACCAGCT AAAGAGAACTCCGAAAAGGGTTCTCTTTCGTCCAAGCCAGTGAAAGTGCAGAGCAAAAAGAATACTCCTGCTGTGACGCC TAAAGCATTAGCAACCTGTAAATGTGGTAGGATAATTTGCATATGCCTCAAGTCCTGTAGCTCATTTGGAAGTGACGTTT GTTCGTTAACGGATTGTCAATTAAAGAACATTTCAGGTGCACAAGCGAGCGAGTTAGATCCTCAATCTAATGGGTCTGAC ACTTCAAAGAAGGAGAGAAATGGTAGTCTCAAGTCTCAGCTTGATAAACCTTCAAACAGTGATCTGTTGGGTAATGAGTT GAAAACGGCTTTTCCTGCTCTGCCGCCGGTACCTCCTGAGAAAGCTCTTCGTGCTGCAATACTCAAAGCTCAATATGCAG GAACCATCATAAAAGCTAAACACAGAATAGTCCTTGGCCAGAATAACAAAGCTGATCTGATTAGAATACAGATAGAAAAG GAACAGATGGAAAGAGCACAACGTGAAGAGAAAGCTAGAATTGAAGCCGAGATGAGAGCTGCTAAGGTTGCTGAACGAAT GAGGGCACAAGACGAGTTGAAACAGAAACGTGAGAGTCAACGGCTTGAAATAGCAAAGATGAAAAAAGGCTTCGATTTTG AACGGAACAACCACTCGAAATTGAAGAAAAAATTCGTTAAAGTCTGTGGCTGCTTTAGTCTGACAAAAGCTCGGTTGTTA CTTGAAGAGTTGGGTCTGGTTTTGAAAAATGATTATTGTCCGGAACTGGAAGTGATCGGGTCAGAAAAATTTGATGCAAT GAGAATGGACGATCTTGAGGAAGGAGAGATCCTTTAGAGTTTTCTCTATAAGAAAACCTCTTGGATGTAATTTTTGACCA TAAGGAATGCTTTGTAACCTTAACTTTGGTTTTTTTTTTTTTTTGTGAACAACAAC ...............................................................................N ....................................i...................................i....... ................................................................................ ......i....................................................................N.... ...............................................N........................N....... .........N.................................i.....................N.............. ..i....................................N...........N.....N..............N....... .............N....N.....................N.............................N......... .........i............i..............................N.......................... ................................................................................ ................................................................................ .....................i....................N..................................... .....................................................................N.......... ...................N.....................................................N...... ..........................................................................N..... ................................................................................ ......i............................................N..........................N. ..........................................................N..................... ................................................................................ ............................N............................................N....N. ....N...........................................................N............... ......N................................................. Pos Score Pred ------------------------ 80 0.427 - 117 0.780 Yes 153 0.751 Yes 247 0.501 Yes 316 0.326 - 368 0.148 - 393 0.403 - 410 0.214 - 444 0.661 Yes 466 0.093 - 483 0.839 Yes 520 0.406 - 532 0.065 - 538 0.100 - 553 0.094 - 574 0.197 - 579 0.352 - 601 0.115 - 631 0.321 - 650 0.647 Yes 663 0.570 Yes 694 0.388 - 902 0.685 Yes 923 0.403 - 1030 0.306 - 1060 0.396 - 1114 0.277 - 1195 0.367 - 1287 0.815 Yes 1332 0.393 - 1359 0.408 - 1419 0.403 - 1549 0.148 - 1594 0.126 - 1599 0.194 - 1605 0.386 - 1665 0.052 - 1687 0.097 -

Explain the output -- Processing locus 277: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATCTTTTCTCTTCATCCTTTTTGTTGTTCCTTGTGATTTTCGAGCAATTGGAGATTAGTGTGGGTGTATCCGGTCTGA TCTAACCCTCCTTGGTCATGATTTTATATGTATGCCCCTTTTCAGACCTCAAAGCTGCCGCCTTTGGATCCGATGGAATC ACAAGGACCTTCTAATGGTCATCCCATGGATATCAGCAGCAATTCAATCCCATCGTCTGCCTGTAATCTGTGCGGAAGAG TCGTTCAATCTGCTTCCGATGATCTTGAAATCTTCAGCTTATGTGTCGACTGTAAGTTTTTGTTGCTTGAAGATTTCGGC ACTCCCGCGCCTCCCTTGGCTCGGCGGCAGACAATTAGGAGGAGGAGGATGAGGAGGAGGAGAAGAACAACTAGGCACGA CAGCTCTGAGTCTGTCGGTGTCGGTGATCTTTCCTCCCAACAATTCACACACTTGATAAGCATTGCAAGACAAAGCCTTT CAACGGTTCAAGCTAGTGAGGATCTACGTGATGACCTGCGAACTAGCTCCCATACCACTCCAAGCGGCTCGACAAGATGG AGACTCTTCTCAGAATCTGAAAGTGATGATTTTGACAACTTTGGCGAAACCGAATCAAACGCTAGCTTTAGCCTCTACAG ATTTCCCCATGCTGATAACGATACCATTTCTTTTAGTGCTTACGGTGGAGAGTCTGATGCTTCCACGGACAGGCATGCCG ATATCTTTGTTCAACCAGATGACAGGAGCGACATTGACTTTGACACTGATATAGACCCGATGCGCGCTGGTCTTAACCAG TGGAACTCAGACGAAGAAGACAGGGAATGGGAGGAAGGAGCGGGTCCTTCTGGGGTTGCAGGAACACGCTATAGAAACTA TTTGGCAAGTCCAAGTGAGAGCTACAGCTCCATGACTCGGTTTGATTCACCTGAGTTAGAGAGAAGCTTTCGTCAAAGAA TTATAGAGAGGAGGCATTCGTTATCTCGCAACATCTTCACTGGTTTGGAGGATTTGGACTTCTCCCCCTATGCTGCAAAT GTTGGGGATTATCTTGATGAGAGAGGCTTTGATGAACTGCTTGAGCAACTTGCTGAGTCTGACAATTCAAGAAGAGGTGC ACCACCTGCATCTGTCTCATGCGTCAGGAATCTTCCAAGGGTTATCATTGCAGAGGAGCATGTAATGAAGGGCCTAGTTT GTGCGATCTGCAAGGAGCTTTTCTCCCTCCGCAATGAAACCACGCAGCTTCCGTGTCTACATCTATACCATGCTCATTGT ATCGTACCTTGGCTGAGTGCCCGTAACTCCTGCCCACTCTGCCGTTATGAGCTCCCGACAGATGATAAAGATTACGAGGA GGGAAAGCGGAATGTGCTGGATGTGAGCGAGGATAGCTCCTCCTCTGATGATGGTACAGAATCGGGGGAAGAGGAATATG TAGAGAGAGGTGAGAGCGAGTCTGGTGTGAGCAGAGTCAGCAGAGGAAGATGGCTGTTTCTGGCTGCTGCACCAGTTGTG AGTCTGGTGGGCGTTGTGTTGGCAATGTGGCTAAGCAGCCCACACAGGAGGGGTATAGCAATCTCTCATAGCCAAAGGGA GAACAGAACCAGAAGATGGCTGCCGTTTTTCTGACTGATGCCCAGATCCGTAGGTGCCTCCAGAATCTGTATTTTTTTAA GACCCTTAAAAGATACTTGAGTTTCGTCTGCTGTTTATCGACTTGGTACTGTT ................................................................................ .................N.........i...N........................................i....... ..............N..........i...................................................... ..................N.....................i....................................... ................................................i............................... ................................................................................ ..............................i.............................................N... .........................N...................................................... ........N...............................................N.................N..... ..................i........................................i.................... ..........................N..................................................... ...............................N................................................ .....................................................................N........N. ................N..............N................................................ ..................N........................................N....i............... .................................i...................................i.......... ..............................................N..............N.................. ...........N........N..........................N..N..........................N.. .................................................i.............................. ........................N....................................................... ...............i.....................N.......................................... ..................................................... Pos Score Pred ------------------------ 98 0.361 - 108 0.548 Yes 112 0.357 - 153 0.508 Yes 175 0.443 - 186 0.568 Yes 259 0.150 - 281 0.751 Yes 369 0.520 Yes 511 0.733 Yes 557 0.362 - 586 0.196 - 649 0.199 - 697 0.204 - 715 0.177 - 739 0.553 Yes 780 0.781 Yes 827 0.287 - 912 0.319 - 1030 0.286 - 1039 0.293 - 1057 0.127 - 1072 0.244 - 1139 0.444 - 1180 0.139 - 1185 0.603 Yes 1234 0.568 Yes 1270 0.564 Yes 1327 0.342 - 1342 0.325 - 1372 0.462 - 1381 0.374 - 1408 0.287 - 1411 0.300 - 1438 0.180 - 1490 0.659 Yes 1545 0.210 - 1616 0.666 Yes 1638 0.176 -

Explain the output -- Processing locus 278: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCATTTTGTGGATTTCATTTGCTTTGTTTTCTTTTATATAATACGATCTGAGACAAGAAAAAAGAAGAGAGAAATATAAA AAAGTGTGTGGAGAGATATAAATTGAGAGGAGTAAATTGGGAATGGTGCGGACATTAGGACGTAAACTAAGCTGGCCAAG ATCTTTTAGTTTTAGAAAGATGTTCGATGGTCGGAACTCTGGACATTCATCTTTCTCTTCTCGTGGGGATGGCATGCATA CACCCGAGCACGAACTCGCCGGACATGCTGCACCATCCACTCGTAGAGGCAAGCAAAATAGGAGATCAGACATGGAAGTG ATGAAGGAAAGATTCGCAAAACTGCTTCTTGGAGAGGACATGTCAGGCGGTGGGACAGGGGAGACCTCTGCGTTAGCTTT GTCAAACGCCATCACCAAACTCGCTGATTCGATGTTTGGAGAGCAGATGAAATTGCAGCCTATGTATCCGGAGACAAAAG AGAATTGGAGGAAAGAAATGGGTTGGTTATTATCTGTGATTGATCACATTGTCCAGTTTGTTCCTTCTAGACAAATGGGT AAAAATGGCCAATTCACTGAGATCATGGTTACAAAACAAAGAGATGATTTGCTGACGAACATTCCAGCTTTACGCAAACT TGACTCGGTTCTTCTCGAAACTTTGGACAACTTCAAGGATCAGAAAGACTTTTGGTATGTGCCAAGAGATATGGAGGATG CAGATCACAATGGAGATTGGAGGAGGGATGAGAACTGGTGGTTACCAGTTGTTAAGGTTCCAACAGATGGCTTGTCTGAG GAATCACGTAGATGGTTGCAGAATCAGAAAGATTCTGTGGCTCAAGTCCTTAAAGCCGCCACGGCCATCAATGCTCATGT ATTGTCTGAAATGCACGTTCCTGAAAACTACATTGATTCCCTTCCAAAGAATGGGAAGACAAGCTTGGGAGATTTTCTTT ACAAGAGCATAACAGAGGAGAGCTTTGATCCTGACTACTTTGTCTCTTTCTTGGATTTGTCAACGGAACACAAAGTACTT GATCTAAAGAACAGGATTGAAGCTTCCATGGTGATCTGGAAGAGGAAGATGTGCCAGAAAGAGAAAGACGGGAAATCTCA GTGGGGATCCACTGTTAGCTTAGAGAAGAGGGAGCTTTTCGAAGTCCGAGCTGAGACCATTTTGGTTATGCTCAAACAAC AATTTCCTGGGATCCCACAATCCTCCCTTGAAGTCTCCAAGATTAAAAACAACAAGGATGTTGGACAGGCGATTTTGGAG AGCTATTCAAGGGTACTAGAGAGTCTTGCTTCAAAGATAATGTCAAGAATAGAGGATGTTCTTGAAGCCGATCGGCTAGT TCAAAGACAGTTGATGGGAGAGGCAGAGACAAGATCAGAAAGCGAGGCAGAATCAGAATACGAGGAAACTGAAAAGGTGG TTGCAGCGGAAACACCAAACTCGAGGAAACTATCAGACTTCATAGGGTGGAGATTGTCATCTGATACTAAGAAGCATAGT TCAATGAGTGATATAGAATTCTTCCACAAAGTTGAGCAGGAGAAGGAGAAGCCCATGATGAAGTCTCCAAGAGCTCTACC GAAGAAATTTTCATATCTAGCAAAGTTAGAGAACATGAGAAGCCCTAGTGACAGACACTGATAGTGAAAAGAGAAGGTAA TAGAACAGGGCATAGAAGAAGAAAAACACAAAGAGAGTGAGAGAGAGAGAATAGAAGAAGGTTAAAGTGTGTGAGAATGT ................................................................................ ..........................................N..................................... ...................i......i.........................................N....i...... ........................N..............................................i........ i......................................i........................................ ...............................N..............N..............N.................. .................i........................................................i..... ....N...................i..................N.................................... ........................................................N.............i......N.. .........i.................N......................................N............. ...........N..........................................................N.....N... ..........i.......................................i............................. ................................................................................ ...........................N....................N............................... ...................................................................N............ .........................................................N...................... .......................................i...............N........................ .............i.................................................................. ................................................................................ ...i..................................................N..i...................... ..................................i............................................. ............................................................................N... Pos Score Pred ------------------------ 123 0.239 - 180 0.516 Yes 187 0.609 Yes 229 0.428 - 234 0.623 Yes 265 0.166 - 312 0.727 Yes 321 0.596 Yes 360 0.712 Yes 432 0.377 - 447 0.130 - 462 0.453 - 498 0.654 Yes 555 0.615 Yes 565 0.208 - 585 0.749 Yes 604 0.303 - 697 0.294 - 711 0.637 Yes 718 0.455 - 730 0.578 Yes 748 0.209 - 787 0.253 - 812 0.356 - 871 0.145 - 877 0.289 - 891 0.613 Yes 931 0.542 Yes 1068 0.486 - 1089 0.400 - 1188 0.178 - 1258 0.351 - 1320 0.747 Yes 1336 0.290 - 1374 0.673 Yes 1524 0.514 Yes 1575 0.287 - 1578 0.517 Yes 1635 0.542 Yes 1757 0.052 -

Explain the output -- Processing locus 279: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAAAGTATTCGTGTTTCTAAAAGTTAGTAATTCATTGGTCTAAATATTATAATGAGGAAAAGTCATTGGTAAGGAAAAG GTTTGAAAAAGGAGCACATAAATAAGGACCAGCGATGCTTCCTTTCACGATGCAAAACACGAAATGAAGAGACACATTCT CTCTACTATATATACCATTTGCTCCTCTTCCTCTAATTCCACACATTCTTCTTCAACTACAAAACAAAAACACTACTACA CATTCTTTCCTCTTGTTCATTTCAAACCCAAAAATCAAAGTTCGAAGTTGAAAGAAGACATGGTTTTGTCTAAGACAGTT TCCGAATCTGATGTCTCAATCCATTCAACTTTTGCTTCTCGTTACGTCCGCAACTCTCTTCCACGATTCGAAATGCCTGA GAACTCAATCCCAAAAGAAGCAGCTTACCAAATCATCAACGACGAGCTAATGCTCGATGGTAACCCAAGGCTGAACCTAG CTTCCTTCGTGACCACATGGATGGAGCCAGAATGTGACAAGCTCATGATGGAGTCCATCAACAAGAACTACGTCGACATG GACGAGTACCCTGTCACCACTGAGCTTCAGAACCGATGTGTTAACATGATAGCACGTCTCTTCAACGCGCCGCTTGGTGA CGGTGAAGCTGCCGTTGGTGTTGGCACCGTCGGATCGTCGGAGGCGATTATGTTGGCCGGTTTGGCTTTTAAGAGACAAT GGCAGAATAAGCGTAAGGCCCAAGGGCTTCCTTATGATAAGCCCAATATCGTAACCGGTGCTAATGTCCAGGTTTGCTGG GAGAAATTCGCAAGGTATTTCGAAGTGGAGCTTAAGGAAGTGAACCTAAGAGAAGACTATTACGTGATGGACCCTGTAAA GGCGGTCGAAATGGTAGACGAAAACACAATTTGTGTCGCTGCCATCCTCGGTTCAACGTTAACCGGTGAATTCGAAGACG TTAAGCTCCTCAACGACCTCCTTGTCGAGAAAAACAAGCAAACCGGATGGGACACGCCAATACACGTGGACGCAGCGAGT GGTGGGTTTATTGCTCCGTTCTTGTATCCGGAGCTGGAGTGGGATTTCCGGCTACCGTTGGTTAAGAGTATTAATGTGAG TGGTCACAAATACGGTTTGGTTTACGCCGGTATTGGTTGGGTTGTATGGAGAACCAAAACCGATTTGCCTGATGAACTTA TCTTCCATATCAATTATCTTGGCGCTGATCAACCAACCTTTACACTCAACTTCTCCAAAGGTTCAAGTCAAGTGATTGCT CAGTACTACCAGCTGATTCGTCTTGGATTCGAGGGTTATCGCAATGTGATGGATAATTGTCGGGAAAACATGATGGTACT AAGACAAGGATTAGAGAAAACGGGACGTTTTAAAATCGTCTCCAAAGAAAACGGTGTTCCGTTAGTGGCGTTTTCTCTCA AAGATAGTAGCCGCCACAACGAGTTCGAGGTGGCCCATACACTCCGTCGCTTCGGCTGGATCGTTCCGGCCTACACGATG CCTGCGGATGCGCAGCATGTCACTGTCCTTCGAGTTGTTATCCGAGAAGATTTCTCTCGAACCTTAGCCGAGAGATTGGT AGCTGATTTCGAGAAGGTTCTACACGAGCTCGATACGCTTCCGGCGAGGGTTCACGCCAAGATGGCTAATGGAAAAGTTA ACGGTGTTAAGAAGACGCCAGAGGAGACGCAGAGAGAAGTCACGGCCTACTGGAAGAAGTTGTTGGAGACTAAGAAGACC AACAAGAACACAATTTGCTAATAAGATTATTTTATAAACTAATATTATTATTTGTTTTGTCCTTAATTGCGCTCCATAAT TATGGACCTTTTTCTTTCTTTGCTGTTTGGATTATTTTTT ....................................................N........................... ..................................N..............N.............N................ ................................................................................ ...........................................................i.................... ..........i.............................................................i....... .................................................i......N....................... ................N...i..........i............N..i.............................i.. ...................................i.........i.................................. .................................................i............................N. .................................N.............................N................ ..................................................................i............. ..........i..................................................................... ..............................................i................................. .........................................................................i...... .............................................N.........................N........ ................................................................................ ...........................................N....i....................i..N....... ................................................................................ .............................................................................i.. .......N........N............................................................... .............................................................i......N........... ................................................................................ ................................................................................ Pos Score Pred ------------------------ 53 0.418 - 115 0.226 - 130 0.203 - 144 0.496 - 300 0.701 Yes 331 0.619 Yes 393 0.807 Yes 450 0.544 Yes 457 0.390 - 497 0.473 - 501 0.571 Yes 512 0.657 Yes 525 0.388 - 528 0.731 Yes 558 0.691 Yes 596 0.536 Yes 606 0.814 Yes 690 0.657 Yes 719 0.476 - 754 0.270 - 784 0.269 - 867 0.859 Yes 891 0.786 Yes 1007 0.796 Yes 1114 0.524 Yes 1166 0.219 - 1192 0.174 - 1324 0.255 - 1329 0.594 Yes 1350 0.548 Yes 1353 0.411 - 1518 0.683 Yes 1528 0.156 - 1537 0.204 - 1662 0.690 Yes 1669 0.322 - 1842 0.051 -

Explain the output -- Processing locus 280: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCGAATTTTGTTGTGTGTGACCTCAAACTGACAACTTCCAGTAATTGTAATATTACCAATTTTTTTTATCAAAAAGATG AACTTCACTTTAGAGCAATAGCAATCCCTCGAGTCTCCCCTATATAAAGATTCCAACTCCTTAACGATTCATCTAATGAA AAAACACACACACCAAAAATATCAGATATCTCAAACAATGTCTTCAGTTTTTGGATCTGTCCATATCTTGGCCATGATAG CCATACAACTCCTCCTTACACACAGTGTTTCATCTCTGAACCTTACCAATGCGTATCTGCACCACAAATGCAGCAACACT CAAGGAAAATATAAGCAGGGGAGCGCGTTCGAGAAAAATCTCAACTTAGTCCTCAGTACCATCACCTCGATTGGAAATTT TCGTGACGGTTTCCGCTACACCGAAGAAGGTGAGGATCCCAATAACGTCTTTGTCATGTTCCAGTGTCGCGGCGACTCCT ACTGGTCCAAGTGCCCCCCTTGCATTAGCACCGCCGTCTCTGGGCTTCGTAGGAGATGTCCAAGAAATAAGGGAGCAATA ATATGGTATGACCAATGTCTTTTAAAGATATCTTCAGTTGCCTCCTTCAATAAGATAGATTATGAGAACGATTTCTATTT GTCCAACCCAAACAACATGAGTGATCGGGGGTTGTTCAACAAGGAGACGTCGGCTCTCCTGGAGAAGCTGGCATATAAAG CCTCCGATAGAAACAACTTGGATGGTAAACAATTGGTGTTGTACGCAGCAGGGGAGAAGAGAATTGGGACAAAGAAGGTG TATGCAATGGTGCAGTGGATTTTGAGGAAGTTTCCACAGTGTTGTGACGGTAAACGAGGAGGGAGAGTTTTCGGTACGAG CTGTAACTTTAGGAAGTCGATCGAGGTATGCTCACCTTCTGGGTTTGATTCCAATTTTTTCTTTGAATTGCATTCGCTTG ACACGCTCTTTGTCCAGGCGATCGCCGAATGGGATCGTTTGACTTATTTGTACGAGCGTTGTGTTCGGAGCCTTTCCGTA GAGGCTACTAACGCGAAAGATGCCCATGAGGCTTTGGCCGTTGAAAAGGAAAAAGTAGCTCAGGCGCTGGCCGACCTGGC AAGGTCTCAAGACGACGCCAAGGAGATGAAGAGGAAATATGACGAGTTAGCTGGTCGCGCTCTTTCGGAGATGAAGAGGC TTCGAGAACGTCGACTTGAATATGCTGAATTTGTCCGGAGATCGGCCCTTGATAAGATGGCGGAGCTCGTTCAGAAGCGA TTGGATCGTATTAAAGCTCATATTGACGATACGAAGGCGGCAGAACCAAAGTTTCTTGAATTCAACCAAGTATCTGGGAA TATCAAGACTCTCGAGGCGTTGGTCGAAGGTGGCGAAGTGGAGATGAAGTCGGATGACACGATGTTAAGATTGGTAGCGG ATACAGAGGAATTGGAGGCCGAGGTGAAAGCTTTTGGTATCACCGATCTTATGGACGGCGACTTCGATGTTCGTACTTTA TTTGCCGAGCTGAGTCCTGATAATCGTAATTCCGTTCCACCGCCGACTAAAGGGGAAGATCTCGCGGATGGACGAGGTGA GTGCGAAGGCCAAGCTGGTGAAAGAATCGTTGGTCAAACTGAAGTTCGAGACGAGATCGCGGATGCTAGAGACTGATATT CCAGGAGGCTAAGCCAAAGGGAAGAAGCGGAGCTCAGATGCCTAAAGAGATCCCAATATGGCAAAACAAAAAATATTCTA AAAACTGAATAATCA .............................................................................N.. ...........................................................................N.... .....................................i...................................N...... ................................................i..................i............ ................................................................................ .......................................................N........................ .......................................................N........................ ..N....i......N..............................................N.................. ................N............................................................... .....................N.......................................................... ...........................N.................................................... ............................N................................................... ...................N.....N...................................................... .........................i............i...............................N......... .....................N..................................i....................... ................................................................................ ...........................................i.........N.......N.................. ..................................................i...............N............. ...................................................................i............ ..............................................................N................. .....................................N...................N...................... ............... Pos Score Pred ------------------------ 78 0.333 - 156 0.135 - 198 0.851 Yes 234 0.420 - 289 0.598 Yes 308 0.679 Yes 456 0.423 - 536 0.300 - 563 0.338 - 568 0.515 Yes 575 0.496 - 622 0.323 - 657 0.427 - 742 0.302 - 802 0.191 - 807 0.284 - 908 0.123 - 989 0.230 - 1060 0.411 - 1066 0.260 - 1146 0.518 Yes 1159 0.706 Yes 1191 0.332 - 1222 0.316 - 1257 0.780 Yes 1404 0.849 Yes 1414 0.169 - 1422 0.328 - 1491 0.717 Yes 1507 0.294 - 1588 0.667 Yes 1663 0.314 - 1718 0.058 - 1738 0.057 -

Explain the output -- Processing locus 281: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACACCGAACATTTAAATAAATAAAAATGAACTTGTGAAAATCATAATTACCAAAATGCCAAATTTAAACACGCCATAAT GACTAACACAAAAAAGGATAGTTTATGCAAATCTTAATTCCACATTTAATTAAACTAATCACGAAAATGTGCAAAACTTT AGTACTGTCATCAGAATATATTATCAGAACGTTTCAATTGGCGGCTTTCTCTCTCTTTCCCCAGGAAGAGAAAGTTCGAA CTCCAGAAATGGCGGTGGATTCTCCCGCTGGAGTTATTATCATCGCCGCCGTGCTTTTACTTGCTGCGACAACCTTGGCC TGCGGCTCCGACGCGGTTCTCAAGCTGGAGAGATTGATTCCGCCGAACCATGAACTTGGTCTGACTGAGCTCAGGGCTTT TGATTCAGCTCGCCATGGAAGGTTATTGCAGTCTCCTGTCGGCGGTGTTGTTAATTTCCCGGTTGATGGAGCCTCCGATC CGTTTCTCGTCGGGCTTTACTACACAAAGGTTAAACTTGGGACACCACCAAGAGAATTTAATGTTCAAATTGACACTGGA AGTGATGTTTTGTGGGTTAGCTGCACTTCCTGCAATGGCTGCCCCAAGACTAGCGAACTCCAAATTCAACTAAGTTTCTT TGATCCTGGAGTGTCATCCTCAGCTTCATTAGTTTCATGTTCAGACAGGAGATGTTACTCAAATTTCCAGACAGAATCAG GCTGTTCTCCCAACAATCTCTGTTCCTATTCGTTTAAGTACGGTGATGGAAGTGGCACTTCTGGTTACTACATCTCAGAT TTCATGTCCTTTGATACTGTTATTACCAGTACCTTGGCTATAAATTCCTCTGCTCCCTTTGTTTTTGGGTGTAGCAATTT ACAATCTGGAGATTTACAGAGGCCTAGAAGAGCAGTAGATGGCATCTTTGGGTTAGGACAAGGGAGTTTATCAGTAATTT CTCAGCTTGCTGTGCAAGGACTCGCGCCACGAGTATTCTCGCATTGTCTGAAGGGAGATAAAAGCGGTGGAGGTATAATG GTTCTAGGTCAAATCAAGAGGCCAGATACCGTCTATACTCCTCTTGTTCCATCACAGCCACATTATAATGTGAACCTGCA GAGCATTGCTGTGAACGGTCAGATATTACCGATTGATCCATCTGTTTTCACAATAGCCACTGGTGATGGAACGATAATTG ATACGGGAACGACTCTTGCTTATCTTCCCGACGAAGCTTATAGTCCATTCATCCAAGCTGTTGCAAATGCTGTTTCGCAG TACGGGCGTCCCATTACCTATGAGAGTTACCAATGCTTTGAAATCACAGCTGGTGACGTTGATGTCTTTCCCCAAGTTAG TTTGAGCTTTGCTGGTGGTGCATCAATGGTCTTAGGACCCCGTGCATATCTTCAGATTTTTTCTAGTTCAGGAAGTTCAA TATGGTGCATTGGTTTCCAGAGAATGTCTCATCGTCGTATAACAATTCTTGGTGATTTGGTTCTTAAAGACAAAGTGGTG GTCTACGATCTAGTTCGCCAACGTATTGGATGGGCAGAATACGACTGTTCTCTGGAGGTGAATGTTTCAGCATCAAGAGG AGGACGAAGCAAAGACGTTATAAACACAGGACAATGGAGAGAAAGCGGTTCGGAGAGTTTTAATAGAAGTTATTACTTGT TACAACTAGTAGTCTTCCTTGTCCACCTTTTTGCTCGTTTCTTGTAGTAGCTTTTAGCATTCACTCCACCAATTATGGTT GGACTTTGTAAGCTTATATTATTGTAATTTAATCACACTAATTAATTCTTACTTACCCACCCACCA ..........................N............................N......................N. ........................N.........................................i............. ................................................................................ ........i....................................................................... .................................................N.............................. ..............N..................................................N.............. ............................................................N................... ....i.............................N............................................. ....................................N..............N............................ .............................................N.................................. ...i............................................................................ ......................................N......................................... .............................................................................i.. ...................................................................N............ .................................................................N.............. ..................................................................N............. ...................N............N............................N.................. .........................N...................................................... .............................N...............................N.................. .................................N.............................................. ..........................................................................N..... .................................................................. Pos Score Pred ------------------------ 27 0.402 - 56 0.424 - 79 0.266 - 105 0.209 - 147 0.548 Yes 249 0.862 Yes 370 0.287 - 415 0.325 - 466 0.337 - 541 0.335 - 565 0.511 Yes 595 0.444 - 677 0.235 - 692 0.116 - 766 0.096 - 804 0.726 Yes 919 0.454 - 1038 0.584 Yes 1108 0.310 - 1186 0.193 - 1267 0.197 - 1300 0.281 - 1313 0.315 - 1342 0.291 - 1386 0.169 - 1442 0.489 - 1464 0.743 Yes 1550 0.389 - 1582 0.195 - 1634 0.193 - 1755 0.120 -

Explain the output -- Processing locus 282: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTTTGATTCGAATCTTCTCGACATGAGTTATATATACGAGCACAACATGGTGCGTGAAACCAATTTTGCCTTCTTGAT GTTTTTTTGTTTTTTGTTTGATTAAACTCGGAAGTGATGATCGATTTTATTGTTTATTGCTTAGATATGAGTTTCCTCAG AGGTATAATTGATTCGTTTAGCTCTATTCTCAACGAAGAATCTAAGAAAGATCCTAGTGTTTCATCATCATCAACTTCTT CTGAGTCCATGAATGGTATCGATGGAGTTCCGGTTACTAACGAACGAATTGCGTATAAGCTTAAAGGATACTTCGATTTA GCGAAAGAGGAAATCGCTAAAGGGGTTAGAGCAGAAGAGTGGGGTTTACATGATGATGCTCTTCTTCATTATAGAAATGC TCAAAGGATCATGAATGAAGCTACTTCTACTCCTTCTCCTTCGTATATTAGTTCCAGTGAAAAGGAGAAGGTGAGATCGT ACCGAGAGAAGATTTCAAATTGGCAAAATCAAGTATCTGAGAGATTGCAAGCTTTGGGTAAGCGTACAGGTGTTGGAATG TCTGAGAATAAGAGGACGGTGGCATATCCTTCTTCAGCTTCAGTTTCCTCTACAGCGTCAAGGTATAGGAAAACTTTATC GCAAAAGACTCCGGTTGCCAGAGGTGGCGTTGCAACGCCAAGGAACCCTAAAGATGCTGCTGCAAGCCCAAAACCTGTGA AAGAATCTGGAAATGTCTATGATGATAAGTTGGTTGAGATGATAAATACAACAATAGTGGATAGAAGCCCATCTGTGAAA TGGGATGATGTCGCTGGACTTAATGGAGCTAAACAAGCTTTGTTGGAGATGGTAATTTTACCGGCAAAACGAAGAGATTT GTTCACTGGTCTCCGAAGACCAGCGAGAGGTCTGCTTCTCTTTGGGCCACCTGGCAATGGGAAAACAATGCTTGCCAAGG CAGTCGCTTCTGAGTCACAGGCAACATTTTTCAATGTCTCAGCATCTTCTTTGACATCAAAATGGGTGGGTGAGGCTGAA AAGCTAGTTAAAACGCTGTTTCAAGTTGCAATATCCAGACAACCGTCTGTTATATTTATGGATGAAATAGATAGTATAAT GTCAACAAGGTCAACCAGTGAGAATGAAGCAAGCAGAAGGCTGAAATCAGAATTTCTTATCCAGTTTGATGGTGTGACTT CTAATCCTGATGATTTGGTGATTATCATTGGAGCTACTAACAAGCCACAGGAGTTGGATGATGCAGTGCTTAGGAGATTG GTGAAGAGAATATATGTACCATTGCCGGATTCCAACGTCAGAAAACTACTTTTTAAAACTAAATTAAAGTGCCAGCCTCA CTCGTTATCCGATGGCGACATTGATAAAATCGTCAAAGAAACCGAAGGATACTCAGGAAGCGATCTCCAAGCATTGTGTG AAGAAGCTGCAATGATGCCAATCAGAGAACTCGGTGCAAATATTCTAACGATCCAAGCAAATAAAGTAAGATCTCTACGA TATGATGATTTTCGGAAATCAATGGCAGTGATCAGACCAAGCTTGAGTAAAAGCAAATGGGAAGAGCTTGAACGTTGGAA CTCAGAGTTTGGCTCTAATTGAATTTCTTCTTGGATTGTTTGTTTCTGTTGTAAATCTTAGGTAGTTGTACTTTTTTTTT TTTTTTTCTCATAAGTGAAATATTGTATTAAAGAAACGAGA ........................N.......................N.............................N. ....................................N.............................i............. ................................................................................ ........i...N........N.......................................................... .................................................N..N..N....................N... ..........i...N................................................................. .............................................................................i.. ................................................................................ .....................................................N.......................... ............N.....N..N................N........................................i ....N..N..............N.........................N............................... ........................................................N..........i............ .................................N...........................i.................. .........................................................N...N................i. .......................N............................................N........... .........N...............................................N..N................... .............N.................................................................. ...........i.................................................................... ...........i..i................................................................. ................................................................................ ......................................... Pos Score Pred ------------------------ 25 0.156 - 49 0.250 - 79 0.086 - 117 0.425 - 147 0.582 Yes 249 0.670 Yes 253 0.484 - 262 0.382 - 370 0.148 - 373 0.141 - 376 0.067 - 397 0.195 - 411 0.579 Yes 415 0.306 - 558 0.634 Yes 694 0.166 - 733 0.259 - 739 0.120 - 742 0.111 - 759 0.454 - 800 0.674 Yes 805 0.230 - 808 0.363 - 823 0.141 - 849 0.230 - 937 0.376 - 948 0.556 Yes 994 0.133 - 1022 0.723 Yes 1098 0.295 - 1102 0.144 - 1119 0.533 Yes 1144 0.212 - 1189 0.154 - 1210 0.101 - 1258 0.080 - 1261 0.125 - 1294 0.214 - 1372 0.570 Yes 1452 0.630 Yes 1455 0.677 Yes 1522 0.267 - 1525 0.291 - 1542 0.363 - 1577 0.196 -

Explain the output -- Processing locus 283: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCCCATTATGCTGATAAACATTTTGGTTGTTTCTTGGGGCAATTTGTGCCAATTGCTTTCAAGGTTTTGCAGATTGTGTT CTTAGACGAAAAGGGTTTTGAGGTGTCATGGATTCCGGGACGCAGCCAGAAGGGTTGCAGGCAGCACAGCCAGCAGGGTT GCAGGCAGCAGGGTTGCAGGCAGCACAGCCAGCAGGGTTGCAGGCAGCACAGCCAGCAGGGTTGCAGGCAGCAGGGAAGC GGAAGCCGCCTACGACGACCGAGGATCCAAATGATCCACAGCATATAATCCACGATTTCCTCACAATAGTGAACAAATTG GAGAAGGAAGTGGATCTTCCAGGCGCTATAACCAGGGCTAGAACCTATGATGCTGAGGTTGATGGACTGATAACTGCTGA CCAACCACAGACGAATCTGGGAAGAGCAGAAAGTGCCCCTATTCCTAATGCTAAGCCAGAAGGCCCAGATGATCATCATA TTCCTAAGCTGCAACGAAATCTTCGTTTGCTTAAGGAGGACGTGGCTAAGCTGCAATATCTCCGCAGTGAAGTCGCAGAG GAGGTGTGGAAACATATTCGTCCACTTGAATTGCTGCTTGAAAATGTGGCAAAAGAGCAGAAGAAAAACCCTACGAAACC CCCCTTGACCTTAGGTATGAAGAAAGACTTGGACGATATCAACAAGAAGATCTTTAACTTGATGTGTCAAGTCCCCTTGT TGCCCAACAAGCGTAAAAAAGCCAAAGGATTGGATGATGACGATGGCGACGGGGATAATAATAACGGCAAAGGAATTGTT TGCCTGCCCGGGATCCATGCAAATAAAGCAGATCTTAGAAGTCGCGCGGTTTTCAGATATGTGCTACGCGAATTCGAGGA ACTTAGTGATCAACGGAAGATCTGCCTGCTGACCTTCGCTGTCTTCCCTGAAAATCAAGAGGTGAATAGAACGATGCTGA TGTATTGGTGGATGGGGGAGGGCATTCTGAGTACTAAAGACATATCATCACAAGAGGGCACTGAAGAGGTCATTCTCAAA CCCGAAGATGTTGTGAAGGTCATTCTTAAGGATTTCACGGATAGAAACTTGATTGAACCTGTTGAAATCAAACGCAAAGT CGAGCCAAGTAGCTATAAGATGGCCCCCTTTGTGCATGCTTCAGTGGTTCTTATCTCCAAGGAGATAGGGCTTTTTGATA TGTATGATATAAAGGACAAGCCAGTCATGAAGAAATCAGGAATGCACAAGGTATGCCTTGTGGAAGGGTCGTCAAGCCAG CAAGAAGCAAAAGCTAAAAAAATGGAAGATGTAGATCATATTGAAACTGTGTTCAATGTTTCGGAGAGGTTTCCGGATTT CACATTCAAGTGGTTCTCGGAGGATAAACCAACGCGAAATAAGTTGACTCTGTCAAAAGTTACGTATCAAAAGCTCAAGG TGTTCTATCTGGGAAGATGGGAGAGAACTGCCAAGCGCCACATCGAGGTGGAGAATCCTGAGCTTATGAAAAATTTGAAG CGTATGATTAAACTCAAGCTTCTGAGTTTTCAAGGGATCTCAAGAATTGAACGACTTGACGATGCTGTCTGCAAGCTTCG TGACCTGATCATCTTGGACCTCAGAGCTTGCTATAATCTGGAGAAACTTCCAGACAAGATAGATTCACTCAAGGCGCTTA TCTACTTGGACATTACAGATTGCTACATGATAGATCGCATGCCCAAGAGGTTGTCATGGCTTGATAACTTAGAGGTTCTC AAGGGCTTTGTTGTCAGTGATGCTACAGATGAAGAGACGGTCTGCACACTGGCTGAGTTGGTGCACTTGAAGAAGCTGAG AAAGCTAAGTATTTCAATAAACAAAGAAAATTTCAGCATAGATGATGTGTTCGTGGCTGTAAAGAGTTTCAAAAAACTGG AAAAGTTGAAGGTTGCGTGGGGAGGTATAAATACACATCCTCAGGGCAAAGGCGTAGATAGCGAAAAGGGGGACGAAAAT GTTAAGCCAAAGGAGAATATTGGGATCTTGGAAAGGGCTGCAACCATGTTCCGTAAGGAGAAAGATCCCACAGCGCCAGA GCTTCCAAAGACATTGAAGAAACTGGACCTGCAATGTTTTCCTGGTGAACATCTTCCTGAATGGCTTGAGCCTGATAATC TACTTAATGTGGAAAAACTCTACATTAAAGGAGGAATCAAACTTACTGGATTTGGAAAGTCACTACCTAGCCAGAATTCG AAATGCAAAGTTACCGTTTTACGTCTGAAGTTTCTTCCAAAACTAAAAGTGGAGTGGAGGGATTTGAGCAAAAGGTACTT TCCGGAACTCAAGTTCTTGGAGAAGTATCAGTGCCCTCAAGTTAGTCTCTGCCCCTGCGATGGAATTGGAATCTGGCGCA AGCCTGAAGAAGATGAAGTCTAAAGCATCTCAGAACTGAGAGTTTCTCGGTTTATCAAGTTTTCTTCCTCGTTGTCTTTT TCTTTTGTTGTTGTTGTTGTTGTGTGTTTGCTTCCTCATTTC .......N........................................................................ ...........................i.................................................... ................................................................................ ..............................N................................................. ..............................................N..N...........N.................. ...............................................N....................N........... ................................................................................ ...........................................N.................................... ................N............................................N.................. .................................N..N.....i..................................... ................N.........................................N..................... .........................................................................i.....i ...........i.................................................................... .......N........................................................................ ...................i...............N...........................................i ...N......................N..............i..........N........................... .....................i......N..........................N........................ ................................................................................ ................i................................................N.............. ...N.........................................................N.................. ................................................................................ ..........................i...........i................N........................ ...................N........N................................................... .........................................N..N................................... ..............................................................................N. .............................................i.................................. .................................N..........................N................... ......N......................................................................... ..i............................................................................. ...........................................................N.................... ............N................................................................... .......................................... Pos Score Pred ------------------------ 8 0.197 - 108 0.710 Yes 271 0.298 - 367 0.300 - 370 0.367 - 382 0.116 - 448 0.182 - 469 0.124 - 604 0.133 - 657 0.383 - 702 0.470 - 754 0.416 - 757 0.308 - 763 0.573 Yes 817 0.114 - 859 0.217 - 954 0.603 Yes 960 0.578 Yes 972 0.538 Yes 1048 0.134 - 1140 0.728 Yes 1156 0.095 - 1200 0.507 Yes 1204 0.153 - 1227 0.465 - 1242 0.751 Yes 1253 0.306 - 1302 0.742 Yes 1309 0.227 - 1336 0.163 - 1457 0.624 Yes 1506 0.141 - 1524 0.385 - 1582 0.267 - 1707 0.644 Yes 1719 0.683 Yes 1736 0.398 - 1780 0.089 - 1789 0.166 - 1882 0.334 - 1885 0.376 - 1999 0.229 - 2046 0.528 Yes 2114 0.371 - 2141 0.439 - 2167 0.269 - 2243 0.735 Yes 2380 0.148 - 2413 0.154 -

Explain the output -- Processing locus 284: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGAAAAAGATTATGTGAATTGTCCTTATCTCAATAACTTCCATGAACTTACCTTCGTTAGCCTCCATCTCTTATGGCTC CCCACATACTCTGTCTCCTAGTTAGCCTCCTCTCACTATTTTGTCTCTTTTGGTAATATTCAACAATTCAAACACGCCAA CTTTGAATATTCCGTAATATTTTTTCCATATTCAACAATTTTGCAAAAACGAAGACTTCTTACTCTCTCAATAGTCTTCA ACTGCCTACCCTTACTTATATTTATGTTAAGATCTCTTAGTCATCATTATATTCATATCCCTAGTTCAGTTTTATCTCCA AAGAACAAAAATCATTCTTTTATTCTTTATTCTTTCAAGCTTCTCTGTTTCTTGTCTAAACAAACACCAAGGAACGTTTG TTTTGTGTTTGATACCCACACAAGGTACTTTTATTTGTTAAAATGTTTGAATGAGACAAATAATTGGTTTGGTATAGCTA ATTTTAATGCGACTACTGTTTGTTTAATTAATGTGTGCAGGGGATTTTCTATATTAAATATACTTTGGAATTATCCTTTC GTTATATTAGTGGTCATGGGTCTTGAAGTTGGGTCCTTATGCTTCAAACTTAAAGACGGAGGCTTGACATCAAGAACCAA CAGTTTCAAGAGAGACGATACTAATAGGCACCAGAATTCTCCTAAGAGTACTATGGAGCGGTCGTTGAGTTTCAACAGCT GGGAGGTTCCTAAAGAGACCAAGACTGATTCAGATTTTGAGGTCTTGGAGACAAAGAAGTCAACGCCTAACACTTTGAAT GGAAGAAACTGTGAGAGAATCCAAATCAAGAAACCTACAGTTACTCCACCAGAGCCATTTGTGTTCTTCTCTCCTAGACC TGTCACCGAGCTTGATGCAGCTGCAACTACGTTACAAAAGGTGTACAAGAGTTACAGGACCAGAAGGAACTTAGCAGATT GTGCGGTCGTTGTTGAGGAGCTCTGGTGGAGGACTTTGGAAGGTGCAGCTTTGGATTTGAGCTCTGTGTCTTTCTTTGGA GAAGAAAAACATGAGACCGCTGTTTCGAAATGGGCACGAGCTAGAAAACGAGCTGCTAAGGTTGGGAAAGGCTTATCTAA AGATGAAAAGGCTCAGAAATTAGCTCTTCAGCATTGGCTTGAAGCTATTGACCCACGTCATCGTTACGGCCACAACTTGC ACTTCTATTATGATGTCTGGTCAGCGAGCAAGAGCACACAACCATTCTTTTACTGGTTGGATATAGGAGACGGCAAAGAT GTAAATCTTGAGAAACACCCTAGAAGTGTTCTGCAAAAACAATGCATCAGATACTTAGGACCGATGGAGAGAGAAGCATA TGAAGTGATAGTAGAAGATGGGAGACTAATGTATAAACAGGGCATGACTCTGATCAATTCAACAGAGGAAGCCAAGTCGA TTTTTGTACTTAGTACTACTAGAAACTTATACGTAGGGATTAAAAAGAAAGGTCTTTTCCAGCACTCTAGTTTCTTATCT GGAGGTGCCACAACCGCAGCAGGAAGGTTAGTCGCCCGCGATGGGATCCTTGAGGCTATATGGCCATATAGTGGACATTA TCTCCCAACAGAAGACAACTTCAAGGAGTTCATAAGTTTCTTAGAGGAGCACAATGTTGATCTCACCAATGTTAAGAGAT GTTCTGTGAATGAGGAATATTCGTCATTCAAATCGACTGCAGATGAAGAAGAAGAAAGGAAGGAAGTCTCAGAAGAAGTT GAGATACCTTCAGAGAAAGAAGAGAGAGCGAGACCAGTGTTTGATCCTGTAAAGAGACTGTCTTGTAAATGGACAAGTGG ATATGGTCCAAGAATTGGGTGTGTTAGAGATTATCCAATGGAGCTACAAGCACAAGCACTGGAGCAAGTCAGTCTGTCTC CTAGGGTTTCACCAGCTAATTCTTACGGACCAATTCCATCTCCAAGGCCTAGTCCTAAAGTGAGGGTGTCTCCACGCTTG GCATACATGGGAATCCCAAGCCCTAGGGCTGTAAAGTGTTAAAATTTAAAACACATTTTTTAAACTTGTTATCCAAGAAA GAGTGAGGTTATAAGAAGAGAAGAGAAACTAACCTCCATTTCTCTTTCTACTTTAATTCCT ............i.............................N..............................N...... ................................................................................ ................................................................................ .......................N........................................................ ................................................................................ ..........................................N.......N............................. ......N.......................N................................................. ...............i......................N......................................... ....................................................i........................... ..............................................................................i. ................................................................................ ..............N................................................................. ................................................................................ ..........N..................N.................................................. ..N............................................................................. .........N..i.................................................................i. .........................................i.....................N...............N .................N..........N..............i.................................... ................................................................................ ........................................N..................N.................... .....................................................i..............N.........i. .........N................................N..................................... ....................................................................N........... ..N..................................i.......................................... ................................................................................ ......i......................................................................... ............................................................. Pos Score Pred ------------------------ 13 0.527 Yes 43 0.167 - 74 0.208 - 264 0.107 - 443 0.482 - 451 0.142 - 487 0.107 - 511 0.104 - 576 0.622 Yes 599 0.196 - 693 0.686 Yes 799 0.500 Yes 895 0.237 - 1051 0.366 - 1070 0.242 - 1123 0.409 - 1210 0.275 - 1213 0.524 Yes 1279 0.537 Yes 1322 0.600 Yes 1344 0.304 - 1360 0.197 - 1378 0.245 - 1389 0.381 - 1404 0.531 Yes 1561 0.445 - 1580 0.418 - 1654 0.533 Yes 1669 0.288 - 1679 0.511 Yes 1690 0.355 - 1723 0.164 - 1829 0.323 - 1843 0.405 - 1878 0.631 Yes 2007 0.645 Yes

Explain the output -- Processing locus 285: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCACCAAATCAAACCCAATTTCACATTGAGAGCCAAACACGAAACTTATGCGTCTTAAATCCTTCTTCCCTCATTTCAC ATTCTTCACCTACTAAATTAACTAACAACTTCATTATTTCTTATCTCTCTTTCTCTTTCTCTCTGCAAAAAAATTATACA CACTCTGTTTCTTTGTTCCTTCTTTGTCACTATGAATCTTAGTTTTTCTTCTTATAATCTCACGGCTACTGCCGTGTCCT CTTCTTGTTTCGGAAGCGACGAACTTCTGGTTTCAAGGAGGCTGTTTTCCCTGAGAGATGTTCAGATTCTTGAGCTCTTT ATTGCCTTTTTCGTGTTTGCGACAATACATTCCTTGCGCCAGAAGAAACACCAAGGGATGCCCGTTTGGCCGTTGGTTGG CATGCTTCCTTCGTTGATATCCGCGGTACGAAGCAACATATACGAATGGCTGTCTGATGTTCTTATCAGCCAAAACGGGA CATTCCGGTTTAGAGGTCCTTGGTTTAGCACCCTCAACTGCGTTGTTACCTGCGACCCAAGAAACGTTGAGCATCTTCTC AAGACCCGTTTCTCTATCTACCCAAAGGGTTCTTACTTCCGTGAGACCATGCAAGATCTACTCGGAGACGGGATTTTCAA CACCGATGACGGAACGTGGCAACGACAAAGGAAAGCAGCAAGTGTCGAATTCCATTCGGCTAAATTCAGACAATTAACCA GTCAATCATTGCACGAACTCGTGCATAATCGACTTTTGCCGGTTCTAGAAACTTCCGGAAAGATTGATCTTCAGGACATT TTGTTAAGACTAACGTTCGACAATGTATGCATGATTGCTTTCGGGGTCGATCCAGGCTGTCTTAGTCCAAAACTACCCGA AATTCCTTTTGCAAAAGCCTTCGAGGACGCAACAGAAGCAACAGTTGTGAGATTTGTGATGCCTAAATTCGTGTGGAAGC TCATGAGGTCTCTTAATTTAGGAACGGAGAAGAAGCTAAAGGAATCAATAAATGGTGTGGATGATTTCGCTGAAGAAGTA ATCCGGACGAGGAAGAAAGAGATGTCTCTAGAGACTGAAATCGCGAAGAGACCTGATCTCTTGACTATTTTTATGGGTCT ACGAGACGAAAATGGACAGAAATTTTCAGATAAGTTCTTGCGTGATATTTGTGTGAACTTCATACTTGCTGGGAGAGACA CTTCCTCTGTGGCTCTAAGCTGGTTTTTCTGGTTAATCGAGAAGAATCCGGAAGTGGAGGAGAAAATTATGATGGGAATT TGCAAGATTCTTGAGCAGAGGGTTGATCATGGAGATACAAAGAAGAACATGGAATATGAGCCCGTCTTCAGACCAGAAGA GATCAAGAAAATGGATTATCTACAAGCTGCTTTGTCTGAGACTCTTAGATTGTATCCCTCTGTTCCAGTCGATCACAAAG AGGTACTTGAAGACGATGTATTTCCAGATGGGACGAAATTAAAAAAAGGAGAAAAGGTTATATATGCAATATACGCGATG GGACGTATGGAAACAATTTGGGGGAAAGACTGTCGTGAGTTCAAGCCAGAAAGGTGGTTAAGAGACGGCAGGTACATGAG TGAGTCGGCCTATAAGTTCACGGCCTTCAATGGTGGTCCTCGGCTATGTCTAGGCAAGGATTTTGCGTATTACCAAATGA GATATGTAGCGGCCGCCATTATTTACCGGTATAAGGTTAGGGTTGATGATAAGGGTGGTCATAAGGTTGAGCCAAAGATG GCTCTAACTATGTACATGAAGCATGGCTTGAAGGTGAATATGGTCAAGAGGAGTGTCTCTGAGATAGACCACTACTACTG CTGAGTGCCTGACATAAAGGGTATAAAATTGGTGTATATATAGTGGTTCGTGTTTTGAGATTTATGAATTGTAACAGGTG ATTGTTTTTTGGTGATTTTGATT ................................................N............................... ................................................................................ ...............................N................................................ .........................................................N...................... .........................................................N...................... ................................................................................ ................................................i............................... .....N.......................................................................... ................................................................................ ......................i...N...i................................................. ..........................................................i..................... ..N................................................N........N................... .....................N..................................................N....... ...........i.................................................................... ....................................................................i..N........ ............................N...................N......N........................ ..........i..................................................................... ...............N...........N...................................N.............N.. ......N....................................................................i.... .............................N...............i..............................i... ...N.........................................N...............................i.. .........N.....N......N................N........................................ ...............................................................N................ ....................... Pos Score Pred ------------------------ 49 0.333 - 192 0.387 - 298 0.111 - 378 0.463 - 402 0.144 - 446 0.766 Yes 457 0.234 - 609 0.504 Yes 646 0.218 - 823 0.710 Yes 827 0.146 - 831 0.584 Yes 939 0.671 Yes 963 0.426 - 1012 0.307 - 1021 0.074 - 1062 0.490 - 1113 0.383 - 1132 0.644 Yes 1269 0.695 Yes 1272 0.410 - 1309 0.152 - 1329 0.409 - 1336 0.183 - 1371 0.806 Yes 1456 0.142 - 1468 0.271 - 1504 0.434 - 1518 0.450 - 1527 0.346 - 1596 0.516 Yes 1630 0.313 - 1646 0.504 Yes 1677 0.619 Yes 1684 0.270 - 1726 0.111 - 1758 0.592 Yes 1770 0.373 - 1776 0.255 - 1783 0.401 - 1800 0.107 - 1904 0.039 -

Explain the output -- Processing locus 286: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGCCCAACAACCTCCATTTTCCTTCAGCTTCAAGGCTCATCATCATTACTACTTTCAAATTCACAGCTCGATTACTAGT ACGACATAGACATAGACATAAAGACAGAGAATGGACCCAAGTAGTATCAACAGCAGCAGAAGAAAGAGAATTGGAGGAGC CGTTGTTGCTGCAGGTTCTGTTTGGGAAACGAGAATGCAGAGCGATGATGAAGTTTGTAATAACGCCATTGTTCTTCATG TTACAAACGAAGACAAGACTTCTTCTACTAAGCAAATGACTTTAAAGGATGAAACTTTATTAGGTGTTAATGGAAAGAGA CGGACTTGGAAGTCTAGAGCGTCCGATGTGAAGAACCCATCAACGCTTGAGATTGTTCGAGCCAATTCTCTGAAGGTGAC GAAGCCGAGATCAATTGGTACTCCTCCGATGACGCCGAGGAGATCGTTGTCTAGTAGTGATTCTAATGATAAGAGTCCGA GTGTGTCGGTGGTGGCGAAGAAGGCAAGATCTGAATCTGTAGAGGGGATTGAGAAGAAGACTACTCCGGGTCGAGTTAAG AAGATCCGATCGGAGGTCTGCACGACGATCGTTAAAGCCGGTGAATTTGATTCAGTTGCGTTGAGGAAAGTGAATTCGTT GCCATCTCCAAATTCTGAGAAATCTGATACGAAAACAGAGCAAGAAGTGACTATCATTGAGAATTCGAAGATCCCAGAAG AAGTCAAGGAGTTTGGTGTTTGTCAGGAGATGATCGTATCTGCTAAATCAAACGAAAATGAGCAGATTGACAATGGCGAC CAGGAGATTGGTGATCAAGATGATTATGAAGAAGATGGAGATGAGGAGGAGGAAAGAGAAGTGGAGAAGAAGAGTGTTGA TGTGAAGGAGATAAATGTAGCCAAGGAAAACAGAGTTGGTGGTGTTGAGATTAAAAAGTTTAGTCAATTTCAAAACAGAA CATCTCCATCTCCGTCGTCCGTTCGTAAAATTTCGCCTCCGGTGATCAAAAGGGCAACTTCTGTTTACTCGGCCCCACCA AATTCTACTTCAAGCACAGATAGATTTGCAGAGCAAGAAGACAACTTTACTCATTCACAGAGTAAATTGCAGAGTCTGGT GGATCTGGTGATGTGGAGGGATGTATCAAGGTCGACGCTTGTGTTTGGCTTCGGAACATTCCTCATCATCTCTTCTTCCT ACGCCAATGATCTCAATTTCAGCTTCATATCGGTGGTAGCGTACATGGGACTCATCTATCTCGGTCTCATGTTCGTATTA AAATCACTGATCCACAGGGGAATGGTGGAGGAGGAGAGACATAAAGTGGTTGGAGTTAGAGAGGAAGATGTCAAGAGGAT GCTCAGACTCATAATGCCTTATCTCAACGAGTCTCTGCATCAACTCAGAGCTCTCTTCTCCGGTGATCCATCAACCACTC TCAAGATGGGAGTAGTGTTGTTTGTCTTGGCCAGGTGTGGCTCTTCTATCACTCTTTGGAACCTCGCCAAATTTGGCTTT TTGGGAGCATTTACAATACCTAAAATCTTCATATCATACTCAACACACTTCTCTGCATACGGAAATTTCTGGATGAGGAG ATTCAGGGACGCATGGGAATCTTGCAATCACAAAAAGGCAGTGGCTTTGGCACTTTTCACCTTAGTTTGGAACCTCTCCT CAGTCACTGCCCGTGTTTGGGCAGCATTCATGTTACTCGTCGCCTTCAGATATTATCAACATAAGATGATTTGGACAACT GATCAAGCTGATGATGATGAGGATGACAATGAAGAAGAAGAAGCAGAAGAAGAAAAAGAACAAGTCCCTCCTAAACACAA AAGGGCTCCTCCTCATATGATGATGCCTAATAAACTCAAGAAAATTTCTTAAATATACTAATTGTTTTTCCTCCATATAT AAACAACATTTACTATGTAATCTCTTTAGTTTTTAGTTACTTCTCTATACAAAGCTTACAGTTTATAATGT ................................................................................ ..............................i................................................. ..................................i.........N..N.............................N.. ...................................N............N....................N.......... .........................N...................................................... ............................N....................................N.............. ................................................................................ ................................................................................ ................................................................................ .............................i...........................N..............N....... ...................N.....N........N.....N......................................N ..............N................................................................. ................................................................................ ................................................................................ ..........i.........N........................................................... ......N.....................................i.......................i........... .....................i.............................................N..........N. .............N.................................................................. .....i.......................................................................... ........................................................................N....... ............N................................................................... .............................i...................................i.............. ..........N..N..N.....N.....N................................................... ................N..N..N......................................................... ..............N....................................................N... Pos Score Pred ------------------------ 111 0.768 Yes 195 0.720 Yes 205 0.117 - 208 0.169 - 238 0.092 - 276 0.411 - 289 0.160 - 310 0.367 - 346 0.168 - 429 0.478 - 466 0.136 - 750 0.770 Yes 778 0.238 - 793 0.330 - 820 0.153 - 826 0.080 - 835 0.121 - 841 0.082 - 880 0.078 - 895 0.175 - 1131 0.550 Yes 1141 0.322 - 1207 0.137 - 1245 0.813 Yes 1269 0.505 Yes 1302 0.555 Yes 1348 0.278 - 1359 0.234 - 1374 0.271 - 1446 0.680 Yes 1593 0.403 - 1613 0.247 - 1710 0.737 Yes 1746 0.877 Yes 1771 0.427 - 1774 0.414 - 1777 0.209 - 1783 0.267 - 1789 0.124 - 1857 0.136 - 1860 0.168 - 1863 0.143 - 1935 0.162 - 1988 0.055 -

Explain the output -- Processing locus 287: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAGTGAACCAATTCATGACAATTTCATGCAAAATTACGTGTTATCTAAACAGCTTATTCCATAACCGTGACTAAAAAT AATAATTCATAAATCATTTAATATTTGGTGTATAAAAAAGTATACTTCATGAAGAAGCATCAAACCTGTATTTTATCAAT TACCACACTTTGTCTAAGCAAAATGGATTTATTACTCATATCCTTAACCACTATCATAATCGCCGCCTACATGCAAAATC TACGACGTCGGGGATCCAACATACCGCCGGGACCACCAACGCGATTTCTGGTCGGAAACCTTCATCAACTGAAACCATTA TGGACGCAGTCTTTCTCCGAGTGGTCACAGACTTATGGCCCCATCATATCGGTGTGGTTAGGATCACAGCTAGCTGTTGT GGTCTCGAGCTCTGACTTAGCCAAACAAGTGTTGAGAGACAAAGACTACCAACTCTGTAACCGACACAGAACCGCAAGGA TGACTCAGAACGGCAGCGATCTTATTTGGTCTGACTACGGAGCACATTATGTGAAAATGAGAAAACTCTGTACACTCGAG CTCTTTTCTTTGAAAAGCATAGAGTGTTTCAGGTCGATGAGAGAGATGGAAGTAAGTTCCATGGTTAAGTCGATTTTCAA TGACTTCATGAGCGATGATCAGAAACCAGTGGTGTTGAGGAACTATCTAGATTCTGTTGCTTTGAACATTGTTTCAAGAT TAGTTATCGGGAAAACATTCGAACCCAAAGATGGAAGAGAGTTTAGATCAATTGTAGAAAGGGAGACTCGCTTGCCCGGC GCAACCAAGATGCTTGATTACACCGTTTGGCTTAAACGTCTTTCGTCATGGTTCACTAGCGACAAGGCGTTCATGAAGCA CATGGCTCGGAAAAGAAATTGGTTTAAACGAGCTGTAATGGATGAAGTATATGGAGGAAGAGATCAAAAGTGTTTTGTTC AGAGTCTGTTAGAGTTGAAAGAGAAGGATGAGCTGACCGAGGAGACTGTGATGGGACTGGTCTGGAACATGTTAACTGCA GGAGCTGACACAACCGCCATAACTATTGAATGGGCAATGGCAGAGATGATCAGATGCCCGACCGTGAAAGAAAAGGTGCA GGATGAGCTTGATTCCGTGGTTGGATCCGGACGGTTAATGTCTGATGCAGATATCCCAAAACTGCCGTTTCTGCAATGCG TACTCAAAGAAGCTCTCCGGCTTCACCCTCCAACACCATTGATGCTTCCACACAAGGCCAGCGAATCAGTTCAGGTAGGT GGGTACAAAGTTCCTAAGGGAGCCACGGTTTATGTCAACGTGCAGGCGATTGCTCGAGATCCTGCAAACTGGAGTAACCC AGATGAGTTTAGACCAGAGAGGTTTCTTGTGGAGGAAACGGACGTTAAAGGTCAAGACTTTCGGGTTCTTCCGTTTGGGT CGGGAAGACGGGTGTGTCCAGCCGCTCAACTCAGCCTCAATATGATGACATTAGCACTAGGTAGTTTGTTGCATTGTTTC TCATGGACATCCTCTACACCTCGAGAACACATTGACATGACCGAGAAGCCTGGATTAGTTTGTTACATGAAGGCTCCATT GCAGGCTCTAGCTTCGTCCAGGCTGCCACAAGAGTTATACCTTTAACTTTTAACTGAGATAGATTATATTAATCAACATA AGATAAAGAGAGATTCTCAATTGCGAAAAACAGATGTAGTTATGGCAAGATTTAGGACATGGATG ................N..........N.................................................... ................................................N............................... ......................N...............................................N......... ...............................................................................i ..................................N............................................. ...............................................................................i ................................................N.......N....................... ....................................N........N..............N..................N .......i......N................................................................. ..............................N................................................. .........N.....................................N........................N....... ...........................N......................i.................N........... .............................N......i........i.......i.......................... ..N..................................N......N..............................i.... .........................................i...................................... ...............................i................................................ ..N............................................................................. .........................................N..N................................... ..i.................................N.............................N............. ................................................................................ .................................N.......N................N...N.. Pos Score Pred ------------------------ 17 0.475 - 28 0.217 - 129 0.341 - 183 0.459 - 231 0.155 - 320 0.562 Yes 355 0.425 - 480 0.787 Yes 529 0.104 - 537 0.280 - 597 0.419 - 606 0.381 - 621 0.448 - 640 0.224 - 648 0.570 Yes 655 0.227 - 751 0.313 - 810 0.313 - 848 0.434 - 873 0.210 - 882 0.623 Yes 918 0.740 Yes 922 0.194 - 931 0.282 - 988 0.256 - 1011 0.760 Yes 1029 0.464 - 1070 0.422 - 1077 0.794 Yes 1086 0.653 Yes 1094 0.785 Yes 1123 0.258 - 1158 0.310 - 1165 0.214 - 1196 0.634 Yes 1242 0.811 Yes 1312 0.503 Yes 1363 0.334 - 1482 0.298 - 1485 0.160 - 1523 0.658 Yes 1557 0.466 - 1587 0.497 - 1714 0.051 - 1722 0.048 - 1739 0.056 - 1743 0.031 -

Explain the output -- Processing locus 288: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTTTGATTCGAATCTTCTCGACATGAGTTATATATACGAGCACAACATGGTGCGTGAAACCAATTTTGCCTTCTTGAT GTTTTTTTGTTTTTTGTTTGATTAAACTCGGAAGTGATGATCGATTTTATTGTTTATTGCTTAGATATGAGTTTCCTCAG AGGTATAATTGATTCGTTTAGCTCTATTCTCAACGAAGAATCTAAGAAAGATCCTAGTGTTTCATCATCATCAACTTCTT CTGAGTCCATGAATGGTATCGATGGAGTTCCGGTTACTAACGAACGAATTGCGTATAAGCTTAAAGGATACTTCGATTTA GCGAAAGAGGAAATCGCTAAAGGGGTTAGAGCAGAAGAGTGGGGTTTACATGATGATGCTCTTCTTCATTATAGAAATGC TCAAAGGATCATGAATGAAGCTACTTCTACTCCTTCTCCTTCGTATATTAGTTCCAGTGAAAAGGAGAAGGTGAGATCGT ACCGAGAGAAGATTTCAAATTGGCAAAATCAAGTATCTGAGAGATTGCAAGCTTTGGGTGTTGGAATGTCTGAGAATAAG AGGACGGTGGCATATCCTTCTTCAGCTTCAGTTTCCTCTACAGCGTCAAGGTATAGGAAAACTTTATCGCAAAAGACTCC GGTTGCCAGAGGTGGCGTTGCAACGCCAAGGAACCCTAAAGATGCTGCTGCAAGCCCAAAACCTGTGAAAGAATCTGGAA ATGTCTATGATGATAAGTTGGTTGAGATGATAAATACAACAATAGTGGATAGAAGCCCATCTGTGAAATGGGATGATGTC GCTGGACTTAATGGAGCTAAACAAGCTTTGTTGGAGATGGTAATTTTACCGGCAAAACGAAGAGATTTGTTCACTGGTCT CCGAAGACCAGCGAGAGGTCTGCTTCTCTTTGGGCCACCTGGCAATGGGAAAACAATGCTTGCCAAGGCAGTCGCTTCTG AGTCACAGGCAACATTTTTCAATGTCTCAGCATCTTCTTTGACATCAAAATGGGTGGGTGAGGCTGAAAAGCTAGTTAAA ACGCTGTTTCAAGTTGCAATATCCAGACAACCGTCTGTTATATTTATGGATGAAATAGATAGTATAATGTCAACAAGGTC AACCAGTGAGAATGAAGCAAGCAGAAGGCTGAAATCAGAATTTCTTATCCAGTTTGATGGTGTGACTTCTAATCCTGATG ATTTGGTGATTATCATTGGAGCTACTAACAAGCCACAGGAGTTGGATGATGCAGTGCTTAGGAGATTGGTGAAGAGAATA TATGTACCATTGCCGGATTCCAACGTCAGAAAACTACTTTTTAAAACTAAATTAAAGTGCCAGCCTCACTCGTTATCCGA TGGCGACATTGATAAAATCGTCAAAGAAACCGAAGGATACTCAGGAAGCGATCTCCAAGCATTGTGTGAAGAAGCTGCAA TGATGCCAATCAGAGAACTCGGTGCAAATATTCTAACGATCCAAGCAAATAAAGTAAGATCTCTACGATATGATGATTTT CGGAAATCAATGGCAGTGATCAGACCAAGCTTGAGTAAAAGCAAATGGGAAGAGCTTGAACGTTGGAACTCAGAGTTTGG CTCTAATTGAATTTCTTCTTGGATTGTTTGTTTCTGTTGTAAATCTTAGGTAGTTGTACTTTTTTTTTTTTTTTTCTCAT AAGTGAAATATTGTATTAAAGAAACGAGA ........................N.......................N.............................N. ....................................N.............................i............. ................................................................................ ........i...N........N.......................................................... .................................................N..N..N....................N... ..........i...N................................................................. .................................................................i.............. ................................................................................ .........................................N...................................... N.....N..N................N........................................i....N..N.... ..........N.........................N........................................... ............................................N..........i........................ .....................N...........................i.............................. .............................................N...N................i............. ...........N............................................N....................N.. .............................................N..N............................... ...............................................................................i ..i..................................................................N..N....... .........N..................................N................................... ................................................................................ ............................. Pos Score Pred ------------------------ 25 0.156 - 49 0.250 - 79 0.086 - 117 0.425 - 147 0.582 Yes 249 0.670 Yes 253 0.484 - 262 0.382 - 370 0.148 - 373 0.141 - 376 0.067 - 397 0.195 - 411 0.579 Yes 415 0.306 - 546 0.562 Yes 682 0.166 - 721 0.259 - 727 0.120 - 730 0.111 - 747 0.454 - 788 0.674 Yes 793 0.230 - 796 0.363 - 811 0.141 - 837 0.230 - 925 0.376 - 936 0.556 Yes 982 0.133 - 1010 0.723 Yes 1086 0.295 - 1090 0.144 - 1107 0.533 Yes 1132 0.212 - 1177 0.154 - 1198 0.101 - 1246 0.080 - 1249 0.125 - 1282 0.214 - 1360 0.570 Yes 1440 0.630 Yes 1443 0.677 Yes 1510 0.267 - 1513 0.291 - 1530 0.363 - 1565 0.196 -

Explain the output -- Processing locus 289: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATTTATTAGTGCTTCAATTTGCTTGCATACTATACAGATTTCTCTTATGCGTAGTTTTCTAACTTAATTTCATTTTGA TTTTTGTTGGTATAGGCATAACTTGTTTACCAATTGATTGATACTCTACTCTTTTTGTGCAGGACAACTTCAGAGACAAC AAATCAATCCGAGAAAGCTCCATATCGAACTGCCGAGACATCTTCCAATGTGGAAGAAGACCCGTTTGTAGTCTTGGAGG AGTCTGAATCAACCCCTAGAGAACCCTCCCGTACTGATCCATTGGACGATATTGGTAAATTCAACAGCAGAAAAACTGAT CATTCATCTGTCCACGGAGGAGTATTTGTTGATATTGACCCTCTTGATAATCTAGGAAAACCAGGGCCGGATATGAATAG TAAAGGGAAGAGTCATTTAAGACCACCAGGAAACATCAGTGGCTCCCAGTCACCACCAGTAGAAAGCCCTGGGAGTTACC ATTCTAAGAAAGTTTCATTTGAAGATTTTTTAGAGCCACATAATATGAGTACTCCACCACCTACCAATTCAAATGGAAGT TTCGAGTCGTCTGATGATGTGTGGCTTACTGTATCCGAGATCCCCCTCTTTACGCAACCTACGAGTGCTCCGCCACCTAC GAGACCTCCACCACCAAGACCTACACGTCCTATTAAAAAGAAGGTTAATGAGCCTTCTATACCGACGTCTGCTTACCACT CACACGTTCCTAGCTCAGGAAGAGCTTCTGTAAATAGCCCGACAGCATCTCAAATGGATGAACTCGACGATTTTTCTATT GGCAGAAATCAGACCGCTGCGAATGGATATCCTGATCCTTCCTCTGGTGAAGATTCAGATGTTTTTTCTACTGCCGCTGC ATCAGCTGCTGCCATGAAGGATGCCATGGATAAAGCAGAAGCAAAGTTTAGGCATGCTAAGGAAAGGAGAGAGAAAGAAA ATTTGAAAGCAAGTAGAAGTAGAGAAGGAGATCACACAGAAAATTATGATTCTCGTGAAAGGGAACTTAGAGAAAAGCAA GTGAGATTGGACCGTGAGAGGGCAGAGAGGGAGGCAGAGATGGAAAAAGCCCAGGAGAGGGAGAAAGAGGAGAGAGAAAG AGAGCAGAAAAGAATTGAAAGAGAAAGGGAAAGATTGGTGGCTAGACAGGCGGTAGAGAGGGCTACAAGGGAAGCACGCG AAAGAGCAGCCACGGAAGCTCATGCGAAAGTTCAAAGAGCTGCTGTTGGAAAGGCTACCGATGCCCGAGAGCGCGCCGAA AGAGCAGCAGTTCAAAGAGCGCATGCTGAAGCACGTGAAAGGGCAGCTGCAGGGGCAAGAGATAAGGCGGCGAAAGCTGC GGCAGAAGCTAGAGAAAAGGCTGAGAAAGCTGCAGCAGAAGCTAAAGAAAGGGCGAATGCTGAAGCGCGGGAGAAGGAAA CAAGGGTTAGGGCTGAACGAGCTGCTGTGGAAAGGGCAGCTGCTGAGGCACGTGGAAGGGCAGCTGCCCAAGCTAAAGCT AAGCAGCAGCAAGAAAATACCAATGATCTTGACTCCTTCTTTAGCTCAATTTCTAGACCAAACAGTGCTCCACGACAGAG AACCAACCCTCTGGATCCTTTCCAGGATTCATGGAACAAAGGAGGATCTTTCGAATCTAGCAGGGAATCTTTGCGAGTGC CTCCTGGCCAACCAGAGAACTTGAGAAAGACCTCTTCAGTAACAAACATTGTTGATGATCTCAGTTCAATTTTTGGAGCT TCAGCTTCCCAATCTGGTGGGTTTCAAGATGTTGATGGAGAAACTGAAGAGAGACGACGTGCCAGACTGGAACGCCACCA GAGGACACAGGAGCGGGCTGCAAAAGCACTTGCTGAGAAAAATGAACGTGATCTTCAAGTACAAAGAGAACAAGTTGAGA AAGATAGGATTGGGGTGACTCTGGATGTTGAGATAAAACGTTGGGGTGCGGGGAAGGAGGGAAACCTGCGAGCTCTGCTT TCGACATTACAATATGTTCTTTGGCCAGAATGTGGTTGGCAACCTGTTTCATTGACTGATTTAATTACGGCTGCGTCTGT CAAGAAAGTTTACAGGAAGGCCACTCTATGTATACACCCTGATAAAGTTCAGCAAAAAGGAGCCAATCTCCAGCAGAAAT ACATTGCCGAGAAAGTTTTTGACATGCTCAAGGAAGCATGGAACAAGTTTAACTCAGAAGAACTCTTCTGAGGAAATCAT GGGGCTATGTTTTGAGATGTGTTTTTCGTGTGCCGCCCAAAAGATGATACATTTGGCATTCATTAAATAGCCAAAGATGA TGCCCAAGTT ................................................N............................... ................................................................................ ...............................................N................................ ................................................................................ ........................................................................N....... ................................................................................ ............................................i...........................i....... .............N..N............................................................... ...............................................i................................ .....................................................i...N...................... ......................N...................................N..................... .............i......N....i...........................i.......................... .............................................N.................................. .......................................i........................................ ................................................................................ .....................N......................................N................... ......................N......................................................... ........................................................N....................... ................................................................................ ......................N......................................................... ..............................N................................................. ......................................................i......................... ............................N.....N............................................. .........................................N...................................... ........................N....................................................... .............N...............N.................................................. ...........................N.................................................... .......................N.............i........................................N. ......N.........N..........................N................................N..N .......... Pos Score Pred ------------------------ 49 0.242 - 208 0.476 - 393 0.367 - 525 0.767 Yes 553 0.580 Yes 574 0.257 - 577 0.404 - 688 0.658 Yes 774 0.796 Yes 778 0.204 - 823 0.352 - 859 0.315 - 894 0.808 Yes 901 0.436 - 906 0.670 Yes 934 0.529 Yes 1006 0.348 - 1080 0.644 Yes 1222 0.191 - 1261 0.385 - 1303 0.280 - 1417 0.272 - 1543 0.474 - 1631 0.277 - 1735 0.579 Yes 1789 0.189 - 1795 0.284 - 1882 0.221 - 1945 0.183 - 2014 0.297 - 2030 0.336 - 2108 0.319 - 2184 0.450 - 2198 0.716 Yes 2239 0.134 - 2247 0.332 - 2257 0.088 - 2284 0.068 - 2317 0.064 - 2320 0.056 -

Explain the output -- Processing locus 290: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATGAACCTTAGTTGTGGTTTTTATTTTTGTTAGCTAGAAACTTCAGTGTTTTTTTTCCGCCAATGGTAGTGCTTTGATG ATGGTCCGGCCCAATTCATAGAAGGCCTTGAAAATGGGCCAGACCAATTCAGAGACCCAACAAGCTCGACTGTATACACA AAATTCACAAAAACAGCTTTTGAGAAGTTTTCTTCTTCTTCACCTTATTTTCGGGTATCAAAGCCACAAAACCCTGAGAA TGGCGGCAGCTACTCAATTTCTCTCCCAACCTTCGTCTCTCAATCCACACCAACTGAAGAACCAAACCTCACAACGCTCC AGAAGCATCCCTGTCTTGTCTCTTAAATCCACATTGAAGCCACTTAAACGCCTCTCCGTGAAAGCCGCCGTCGTTTCTCA AAACTCGTCCAAAACCGTGACGAAGTTCGATCACTGTTTCAAGAAATCATCAGATGGGTTTCTCTATTGTGAAGGAACTA AAGTTGAGGATATCATGGAGTCAGTGGAGAGAAGACCCTTTTACTTATATAGCAAACCTCAGATCACTAGAAACCTCGAG GCTTATAAAGAAGCATTGGAAGGAGTGAGCTCTGTGATTGGTTACGCTATCAAAGCTAATAACAATCTTAAAATTTTGGA GCATTTGAGAAGTTTAGGCTGTGGTGCTGTGCTCGTTAGTGGAAATGAGCTTAGACTTGCTCTTCGTGCTGGTTTCGATC CCACAAAGTGCATTTTCAATGGAAATGGCAAGTCTTTGGAAGATTTAGTTCTAGCTGCTCAAGAAGGTGTTTTCGTTAAT GTCGATAGTGAGTTTGACTTGAATAACATTGTGGAAGCTTCAAGAATTTCTGGTAAGCAGGTCAATGTACTGCTGCGTAT CAATCCTGATGTTGATCCTCAGGTGCATCCATATGTTGCTACTGGGAACAAGAACTCAAAGTTTGGTATCAGGAACGAGA AGCTTCAATGGTTTCTGGATCAGGTCAAGGCACATCCCAAAGAGCTGAAGCTTGTTGGAGCTCATTGCCATCTAGGCTCT ACCATTACTAAGGTGGATATATTCAGAGATGCGGCAGTTCTCATGATAGAATACATTGACGAGATCCGGCGTCAAGGTTT TGAAGTTAGTTACTTGAACATTGGTGGTGGTTTAGGGATTGATTATTACCATGCCGGCGCTGTCCTTCCCACACCCATGG ATCTCATCAACACTGTAAGAGAGCTTGTTCTTTCACGAGACCTGAATCTAATAATCGAGCCAGGGAGATCTCTGATTGCA AACACTTGCTGTTTCGTCAACCATGTAACTGGTGTGAAGACGAATGGAACTAAGAACTTCATAGTCATTGATGGAAGTAT GGCTGAGCTTATCCGTCCCAGTCTTTATGATGCTTATCAGCACATTGAGTTGGTCTCTCCTCCACCGGCTGAAGCAGAGG TTACCAAATTCGACGTAGTGGGTCCTGTCTGTGAATCTGCTGATTTCCTGGGCAAAGACAGAGAGCTTCCCACTCCTCCA CAGGGAGCTGGTCTGGTGGTTCATGACGCTGGTGCATACTGTATGAGCATGGCTTCCACTTACAATCTCAAGATGCGTCC TCCGGAATACTGGGTTGAAGAAGATGGGTCGATCACTAAGATAAGGCATGCTGAGACATTCGATGACCATTTGCGTTTCT TTGAAGGTCTATGAACTCTTAGATTTACTCATCATTGTTGCTTTTTTGATTGAATTGTATGATTATTCGGAATCAGGACC ATAATGATATTGCTGTTTTAGATTTTCACATTA N................................N.............................................. ...............................................................................i ................................................................................ ................................................................................ .....................................................i.......................... ..............i................................................................. ................................................................................ ............................................N................................... ..................N.....N.....................................................N. ................................................................N............... ........N.......................N............................................... .......i........................................................................ ............................N.............i..................................... ..................................................N.........................i... ................................................................................ ......................N....................N..........................N.......i. ..........................N..N.................................................. ................................................................................ ......................N...................N.....i.......................N....... .......................N.......................N..............N................. ..........N...............................................N..................... ...N............................. Pos Score Pred ------------------------ 2 0.271 - 64 0.202 - 78 0.206 - 81 0.354 - 114 0.347 - 240 0.824 Yes 454 0.506 Yes 495 0.667 Yes 685 0.233 - 739 0.149 - 745 0.282 - 799 0.100 - 865 0.236 - 889 0.362 - 913 0.227 - 968 0.678 Yes 1069 0.243 - 1083 0.562 Yes 1171 0.223 - 1197 0.542 Yes 1303 0.400 - 1324 0.317 - 1351 0.355 - 1359 0.812 Yes 1387 0.080 - 1390 0.277 - 1543 0.404 - 1563 0.490 - 1569 0.835 Yes 1593 0.338 - 1624 0.288 - 1648 0.240 - 1663 0.481 - 1691 0.128 - 1739 0.073 - 1764 0.048 -

Explain the output -- Processing locus 291: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTCATTACAGCCATAAACAAAGCTCGGTACAACATCAGCCTTCACTGGTAAACTCACTCTTTCCTTGCATCAAATCAAT CAATGTTTTATCGCTAGATAGTCTGCTAATCAGACCATGTGCAGGCATGGGATACGGCAGATGAGGAATCCATGGGCGGA TGGACCAGAATACATAACCCAGTGCCCAATCCAACCAGGAGGGAGTTACACCTACAGGTTTACCATGGAAGACCAAGAAG GTACATTGTGGTGGCATGCCCACAGTAGATGGCTGCGAGCCACCGTGTATGGTGCTCTCATTATCCGCCCTCCGCTCTCT TCTCCTCATTACCCTTTTCCAGTTATTCCCAAGAGAGAGATTACCCTCCTCTTAGGAGAGTGGTGGGACAGAAATCCCAT GGATGTCCTCAACTTAGCCCAGTTCACTGGAGCAGCACCAAACATCTCAGACGCCTTTACCATCAATGGTCAGCCTGGTG ATCTCTACAGATGCTCCAGCCAAGAAACTTTAAGATTTCTAGTGGGGTCAGGAGAGATAGTCCTCCTCAGGGTCATCAAC TCTGCTTTGAACCAAGAACTCTTCTTTGGTGTCGCGAATCACAAACTCACAGTGGTTGCGGCGGATGCCTCCTACACCAA ACCATTCTCGACCAATGTCATAATGTTAGGTCCTGGTCAGACAACTGATGTGCTTCTAACTGCAGACCAACCGCCAGCAC ACTACTACATGGCAGCACATGCCTACAACAGTGCCAATGCTGCCTTTGACAACACCACAACCACCGCAATCCTCAAGTAC AAAGACGCCTCCTGTGTTACTCTTCAAGCTAAATCACAGGCACGGGCAATACCCGCACAACTACCAGGATTCAACGACAC AGCAACAGCAGCAGCCTTCACAGCGCAGATGAAGAGTCCTTCGAAAGTAAAAGTGCCCCTGGAGATTGATGAGAACTTGT TTTTCACAGTGGGATTGGGACTATTCAACTGCCCAACCCCCAACACACAAAGGTGTCAAGGCCCTAACGGAACACGCTTC ACCGCAAGCATCAACAATGTCTCTTTCGTCTTCCCCAAACAAAACTCCATAATGCAAGCCTATTACCAAGGCACTCCCAC AGGTGTTTTCACCACAGACTTCCCTCCAACGCCTCCTGTCACATTTGACTATACAGGAAACGTGAGCAGAGGGCTATGGC AGCCTACTCGCGGAACAAAGGCCTACAAACTAAAGTTCAATTCGCAAGTGCAGATTATATTGCAAGACACGAGCATCGTG ACCACTGAGAATCACCCAATGCATCTCCATGGCTACGAGTTCTACGTAGTTGGGACAGGTGTTGGTAACTTCAACCCAAA TACAGACACATCCTCCTTTAACCTCATCGACCCACCACGAAGAAACACAATCGGAACACCTCCAGGTGGATGGGTAGCCA TCAGATTCGTTGCCAATAATCCAGGAGCGTGGCTGATGCACTGCCACATTGACTCACATATATTCTGGGGTTTAGCAATG GTCTTCTTAGTAGAGAACGGAGAAGGACATTTGCAGTCTGTACAGTCTCCACCATTGGACTTACCCCAATGTTAAGACTG TTATCTACACACATATACACTTGTATATTCTCAAATTTGTTTCACAAAACATGTCATTCATTTTCAATTACAAAAGATAA ACGTATCAAGATAT ................................................................................ ..i.................................i.........i.............N..........i.......N ................................................................N............... ...............N............N...................N............................... ..............................................................................i. ..i..............................................................N.............. ..........i..................................................................... ................................................................N............... ..............i.......i........................N................................ ........i.........N.................N........................................... ................................................................................ ............................N.......................................N........... ................................................................................ ................i..................................i............................ ...........................................................................i.... ................................................................................ ..................N.........i................................................... .....................................................................N.......... ...................................N.........................................N.. ....................................................................N........... ..................................................N............................. .............. Pos Score Pred ------------------------ 83 0.687 Yes 117 0.600 Yes 127 0.753 Yes 141 0.322 - 152 0.702 Yes 160 0.459 - 225 0.471 - 256 0.370 - 269 0.441 - 289 0.464 - 399 0.780 Yes 403 0.777 Yes 466 0.497 - 491 0.673 Yes 625 0.286 - 655 0.666 Yes 663 0.908 Yes 688 0.438 - 729 0.841 Yes 739 0.325 - 757 0.256 - 909 0.465 - 949 0.336 - 1057 0.634 Yes 1092 0.874 Yes 1196 0.710 Yes 1299 0.380 - 1309 0.659 Yes 1430 0.187 - 1476 0.260 - 1518 0.420 - 1589 0.283 - 1651 0.056 -

Explain the output -- Processing locus 292: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATATATTTTGTTCTTTCAAGTTTCAACATTTGACTTCAAAGATTTACTTATAAATATGGAACTCGTACTCTCTTGGGT CCTACTACACTATCTATATAATTCTGCATTGCCCGTTTCTTCATTTTTACCTGCATGACCATGAATTATCCTTACGAGGA CTTCTTGGACCTCTTCTTCTCTACCCACACTGACCCGCTTGCCACGGCTGCAAGCACCTCTTCGAATGGTTATAGCTTGA ATGATCTTGACATTGATTGGGACTGTGACTTCCGTGATGTCATTGAATCCATTATGGGTGATGAAGGAGCCATGATGGAA CCGGAATCGGAAGCTGTTCCAATGCTTCATGACCAGGAGGGACTTTGTAACTCTGCGTCAACGGGTTTGTCTGTGGCTGA TGGGGTTAGTTTTGGTGAGCCTAAGACTGATGAATCAAAGGGCTTGAGGTTGGTTCACTTACTTGTGGCTGCTGCTGATG CGTCAACTGGTGCGAACAAGTCTAGAGAGCTAACTCGGGTTATACTTGCAAGGCTTAAAGATTTGGTTTCTCCTGGTGAC CGAACTAACATGGAGAGGTTAGCTGCTCACTTCACCAACGGACTTTCAAAGTTGCTCGAACGAGATAGTGTTCTGTGTCC CCAACAACATCGTGATGATGTCTATGATCAGGCGGATGTGATCTCAGCGTTCGAACTGCTGCAAAACATGTCTCCATATG TCAACTTTGGTTACCTCACTGCCACACAGGCTATTCTTGAAGCTGTGAAGTATGAACGTCGAATCCATATAGTGGATTAC GATATCAATGAAGGCGTTCAGTGGGCTTCATTGATGCAAGCCTTGGTGTCTAGAAATACAGGCCCGTCAGCCCAACATCT TAGAATCACGGCTTTGTCACGAGCAACAAACGGTAAAAAATCCGTGGCTGCAGTCCAAGAGACTGGGCGGCGTCTAACTG CGTTTGCCGATTCTATCGGCCAGCCATTTTCATATCAACACTGTAAACTTGACACCAATGCATTCAGCACATCATCTTTG AAACTTGTAAGAGGAGAAGCAGTGGTAATCAATTGTATGTTGCATCTTCCTCGGTTTAGTCACCAAACACCCAGTTCAGT CATTTCCTTTTTGTCGGAAGCTAAAACTCTAAACCCGAAACTTGTTACGCTTGTTCACGAAGAAGTTGGTCTGATGGGAA ACCAAGGTTTTCTATATCGTTTTATGGACTTGCTACATCAGTTCTCAGCCATATTCGACTCTCTAGAGGCAGGCCTTTCA ATCGCTAACCCGGCCCGTGGCTTTGTTGAGCGTGTTTTTATCGGTCCTTGGGTTGCTAATTGGTTGACGCGTATCACCGC TAATGATGCAGAAGTCGAGAGCTTTGCTTCGTGGCCACAGTGGTTAGAGACTAATGGGTTCAAACCCTTGGAAGTGAGCT TTACCAACCGTTGTCAAGCAAAACTCCTTTTGAGTTTGTTTAATGATGGATTTAGAGTGGAAGAATTGGGCCAAAACGGG CTCGTTTTGGGATGGAAATCTCGGCGTCTTGTGTCGGCCTCCTTTTGGGCCTCGTGCCAGACGAATCAGTAAATTCTCGT TTTTGCATTCTCGAGTACATTACATAAGATAGTCCCTTTTTTTCCTTTTTTGATATAATGGTTTATGAAGACATCAAATT ATGTGAATATC .........................................................N...................... ......................................................i.....i................... .................................................................i.............. N...................................N................i......N..........i..i..... .....................i......N..................................................N .............................N...............................................N.. ................................................................................ .........i...................................................................... ..............N..N.....N...........N...............................i.........N.. ...................................................N............................ .......N.........................i.............................................. ................................................................................ .........................................................N...................... ....................................N........................................... .........................................................................i...... .......................i........................................................ ................................................................................ ..N..N...............................................i.......................... ..........................................N..i.................................. ...........i.................................................................... .........................................................N......N............... N.......... Pos Score Pred ------------------------ 58 0.395 - 135 0.546 Yes 141 0.697 Yes 226 0.520 Yes 241 0.400 - 277 0.207 - 294 0.824 Yes 301 0.128 - 312 0.654 Yes 315 0.761 Yes 342 0.715 Yes 349 0.119 - 400 0.116 - 430 0.058 - 478 0.147 - 570 0.635 Yes 655 0.128 - 658 0.245 - 664 0.183 - 676 0.105 - 708 0.767 Yes 718 0.395 - 772 0.489 - 808 0.339 - 834 0.683 Yes 1018 0.454 - 1077 0.433 - 1194 0.810 Yes 1224 0.500 - 1363 0.345 - 1366 0.386 - 1414 0.559 Yes 1483 0.439 - 1486 0.683 Yes 1532 0.556 Yes 1658 0.045 - 1665 0.050 - 1681 0.089 -

Explain the output -- Processing locus 293: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCAGCCATGCTCTACTGCTTTCGTTTTCTCAGATCTGGGTTCCATATCTCTGTTTTTTCTGAAGAAGTTGGTGGAGTT AGTTCAGCTCGATAGTCTTAGTTCTACGGTGTCTGTTTTCCTCTTAGCTTGAAGATGTCATCCGCCATGGATAGGGCATT GATGGAGATGTCGTTGGAAGAAAAGGAAGAACCGTTTGAAATGCCAGATCTTCCTGAGTATCTGTCTAGTGAGAGAAACA AATTCAGTCTCATTGGAAGGGTCCTGAACCCGGCTTGTCAACCGATGAAGAATCTCTTAAGAAATATGCCAAGGAAGTGG CAGAAAGAAGGAAAGGTCAAAGGAATAGCTCTCAATAATGAGAGATTCCAGTTCATTTTCGACTCTGAACATGATTTAGA GGAAGTGCTAGCGAAAGGAGTTCATACCTTCAATGATTGGTCAATCGTGGTAGATAGGTGGTATGAAAATCCTCCGGATG ACTATCTTCGTTACATGCTACTGTGGGTTCAAATTTGGAATATCCCAGTCAATTACAACACGGCCAAGGCGATCACTAAG CTGGGTGATCTCATTGGTGAAGTTAAAGAAGTCGTCTTTAACCCAGAATTGAAACAAATTCGGGAGTTCGTTAGAGTGAA GGTGCTGTTTGATGTCTCAAGACCCTTAAGGAGATCAAAAGTTGTGAATTTCAGAAATGGAGGGTCAGCGACTGTTAACT TTCATTATGAGAGGATTCAGAAGAGGTGTTATGAGTGTCAACGGCTTACGCACGAAAAAGATGTTTGCCCTATCCTAGTA AAAGCTAGACAGGACCTAGCTACAGCCAGAAGGAAAGGCATTATCATTCCAAAAGCTACTACTCCTCCGATTCTGAAGGA ATCAGACCCTTTATTTGGTGTGTTGAAAGAAAGCCAGGTGGGAGTGGATCCAAACACGGGAAGGCCGAGAATTGCTCCGG AAGTGTTAGAAGGTATGCGTCAATACCTTCGGGTCGCAAATGCTGAAGAACGATCTGTAAGAGTGGATAGAGTTATTCAA TCAGTTAGTGAAGCAGAAAAGGACCCTTTTACTCAAAAAACAGTTCTGCGATTGGAATCTCTTCCAATTGTTCACCATGA TCTGTCAAAGGAAAAAGGTGTGGTCTTTAGCTTTGATCAAGAAGGTTCTTCGAGTCAAAGTGGCAGAATCAACCTCAACT CGTCTCCTGAGAGGATAGAAGACTCTCAGCCTGAAAGAAACACAAGTTGGCTCAGGGAACCGGTGAAGCCTTTTGTGCTC AATGAAGGAGGTAGTTTCCTTGCTCTCTCTCAGCCTTTTACTGATAGTCCAACGGTTTATAGCCCTGGTCTCTTTGAGAC AGGCTCTACTGGGACTTTTCAGAAAAAAGCAAAGCAGAGAAACAGGCCCCCAAAACATGCAAGGAAACCAAAACCGAGAG ATCCACATTACAATGTTGCTGATCAGAAGATAGGGAAGGGTTCAGCTTCAAGTTCCAAAGACAAGAGAAAAGCAGTTGAT TTGGGCACGAGCACTGCAAAATTCATCAAGCTCAGCTCAACAATGGTGGTCCCAATTGAGGGACCGTCCAACGCCTAATG AGTCTTATTAGTTGGAATTGTCAAGGCTTGGGACGGTCTCATGAATTGACGATTCCTAGACTCATGGAGATGAGACGTTC TCATTTTCCTGAGGTGT ........i....................................................................... ......................................................i...........i............. ............................................N....................i.............. .....................................N................................N......... ................................N.............................N..............N.. ..............N................................................................. ................................................................................ ...........N............................................i....................... ......N.......................N.............................N................... ................................................................................ ................................................................................ ..............N........................N........................................ ............................................................................N... ................................................................................ ................................................................................ ........................................................i....................... ............N................................................................... ..........................................i..................................N.. ........................................N......................N.....N.......... ................. Pos Score Pred ------------------------ 9 0.626 Yes 135 0.770 Yes 147 0.677 Yes 162 0.294 - 168 0.473 - 201 0.581 Yes 285 0.315 - 306 0.554 Yes 358 0.346 - 391 0.173 - 433 0.223 - 463 0.142 - 478 0.088 - 495 0.412 - 652 0.324 - 697 0.817 Yes 727 0.489 - 751 0.384 - 781 0.204 - 975 0.400 - 1000 0.167 - 1117 0.438 - 1282 0.295 - 1417 0.538 Yes 1453 0.466 - 1563 0.680 Yes 1598 0.215 - 1641 0.050 - 1664 0.061 - 1670 0.046 -

Explain the output -- Processing locus 294: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTATGAAATGAAATTTATAATGAAATGATTTGTTTGATAGAAAGAATAAATTATAAACTACTTAAGAGACCGACGAAG ATAGCAACGACAAAACCCTAACCTTATACCAACCCTCATTTAATACCAATAAAAAAAGAGGGACAAAAAAAATCAATGCT ATATAATCGAAGTATCCACTACAAAGGTTGGAGAATCCTTAAACTGACTATGAAAGCGGAAAAAGTTATGGAGAGAGAGA TCGAGACAACGCCAATAGAGCCTCTTAGCCCAATGTCACATATGCTAAGTTCGCCAAATTTCTTCATTGTCATAACTTTT GGGTTTAAAACTCGATGCAACCGATCGGCCTTTGTTGATGGCATCAACAACACATTGATCAATGCTCCCAGATTCTCCAG TAAAATGGAGATAAATTATAAGAAAAAAGGAGAACCGGTCTGGATTCCTGTTAAACTTCGAGTAGATGATCATATTATTG TGCCGGATCTTGAATATTCCAACATTCAAAATCCTGATCAGTTCGTAGAAGACTATACTTCAAACATAGCTAATATTCCA ATGGACATGTCCAAACCTCTTTGGGAATTTCATTTACTCAACATGAAGACATCAAAAGCAGAATCTTTGGCTATAGTAAA AATACATCACTCCATTGGTGATGGGATGTCTCTTATGTCCCTACTACTTGCTTGTTCACGAAAAATATCAGACCCTGACG CGCTAGTTTCTAATACCACGGCTACAAAAAAGCCAGCTGATTCCATGGCTTGGTGGTTGTTTGTTGGGTTTTGGTTTATG ATAAGAGTCACCTTCACCACTATTGTTGAGTTTTCCAAGTTAATGCTTACAGTATGCTTTTTGGAAGATACCAAAAACCC TCTTATGGGTAACCCGAGTGATGGATTTCAATCTTGGAAGGTTGTCCATCGGATAATAAGTTTTGAGGATGTCAAGTTAA TAAAGGACACAATGAACATGAAGGTGAATGATGTTCTTCTTGGAATGACACAAGCAGGTCTTTCAAGATATTTGAGTAGC AAATATGATGGATCAACGGCTGAGAAGAAAAAGATCTTAGAAAAACTCCGTGTTCGTGGTGCCGTAGCTATAAACTTAAG ACCAGCAACAAAGATAGAGGATTTGGCTGATATGATGGCGAAGGGTTCAAAGTGTAGATGGGGAAACTTCATAGGAACGG TTATATTTCCGTTGTGGGTGAAATCAGAGAAAGATCCGTTGGAGTACATTCGACGAGCCAAAGCTACGATGGATAGGAAA AAAATATCTCTGGAAGCTTTTTTTTTCTATGGAATTATTAAATTCACCTTGAAATTTTTTGGAGGAAAGGCAGTAGAAGC TTTTGGAAAGAGGATATTTGGTCACACATCATTGGCATTTTCAAATGTGAAAGGTCCCGATGAAGAAATAAGTTTTTTTC ACCATCCGATTTCATATATCGCAGGAAGTGCATTAGTTGGAGCACAAGCTCTCAATATCCATTTTATAAGCTACGTAGAC AAGATTGTCATCAATCTAGCTGTTGACACAACAACGATTCAAGATCCTAATCGACTATGTGATGATATGGTGGAAGCACT TGAGATCATCAAATCTGCCACACAAGGGGAAATATTTCACAAAACGGAAGTTTGAAACACATCACCTGATGTTTGATCAG GGTTATGTAATATATAACTATATGAAGTTTTGTATTTGCAATGAATTTTTTTATGGAGTATGATAACCAAAATA ....N....N...........N....i..................................................... ...........................................................................i.... .................................................N.................N............ ................................N........N...................................... ..............i......................N.......................N.................. ....N............................................................N.............. ................................................................................ i.....i...................................i..................................... ....................N....i........N............................................. ............................................N................................N.. ..........................................N..........i.......................... ....i...............N...............................................N........... ...........i.....i.........N..N.............N................................... ....N..N........................................................................ ...............................N..i......................i...................... ....................................................................i........... ............................N................................................... ............................................N..............N.................... ................................................................................ ........................................................i....N....i............. ....................................................................N........... ....N................N..................N...........N......N.............. Pos Score Pred ------------------------ 5 0.443 - 10 0.310 - 22 0.325 - 27 0.513 Yes 156 0.723 Yes 210 0.316 - 228 0.451 - 273 0.332 - 282 0.166 - 335 0.518 Yes 358 0.414 - 382 0.113 - 405 0.412 - 466 0.159 - 561 0.571 Yes 567 0.707 Yes 603 0.622 Yes 661 0.454 - 666 0.747 Yes 675 0.266 - 765 0.345 - 798 0.297 - 843 0.238 - 854 0.576 Yes 885 0.622 Yes 901 0.234 - 949 0.132 - 972 0.615 Yes 978 0.660 Yes 988 0.245 - 991 0.307 - 1005 0.210 - 1045 0.216 - 1048 0.131 - 1152 0.370 - 1155 0.500 - 1178 0.778 Yes 1269 0.569 Yes 1309 0.134 - 1405 0.191 - 1420 0.337 - 1577 0.603 Yes 1582 0.137 - 1587 0.595 Yes 1669 0.136 - 1685 0.059 - 1702 0.050 - 1721 0.052 - 1733 0.045 - 1740 0.042 -

Explain the output -- Processing locus 295: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTAATACTTTTTGTCGAACAATTTGCAAAAATATAATTTGGTCGCTATATGATCGCTAGGTTAGAGGTTACCATCAC TGAACAGTGCTTTGGTCGTAAGGTTACCCCATCCGCATTCGTATTATTCTGGATAAGGTTTTATTTTGGGCTCATTTCCC TTTCGTGGAAAACAAAGAAAAAAAAACAATAATGATGATTCACTTTTACTCCTCTTCTCTCCACAGCTCGAAGTTCTTGG ACGGATTCAGATATCATAGCCTATCTGTGATTAGATCTGAAGCACGTAGTTTCAACGTGACCACCACGAGGAAGGAGGTC GTTGTGGCGGCTGCTCCTCTTCTCTCTTTGCCGGAAAACAGGGTTATCCCTCTCTCCAACCTCGACCTGCTTATCCCTCC TGTCGACATCAACGTTTGCTTCTTCTACAAGAAACCACTTTATGGCATTATTGGTGATGCCCTCAAAACGGCTATGGCGG AGGCTCTTGTCTCCTACTACGTTCTTTCCGGGGAGGTCTCCATCAATCCCACCAACGGAGAGAACGAGATTCTCTGCAGT AACGGTGGTGTGGAGTTTGTGGAAGCCGCTGCTGACGTGGAACTCCGAGAGCTTAACTTGTATGAACCTTATCAAAGTAT TGCCAAGTTTGTTCCCATGAAAAAGCATGGAGTCTTTGCTATTCAGGTAACTGAACTGAAATGTGGGAGCGTAGTAGTGG GATGCACATTTGATCACCGTATAGCAGATGCTTATTCCATGAACATGTTCCTCGTCTCGTGGGCCGAGATATCTCGATCC GATATACCGATTTCGTATGTACCTTTGCTCAAAAGATCCCTCTTAAAGCCACGACGACCCTTGATCATCGACTCTTCAAT AGACAAACTGTACATGCCTATAACGTCCTTGACCGTTCCACAAGAAATCACTAATCAAGATAATATCCTAACAAGCCGTA TCTATTACATCAAAGCAGATGTCTTAGAAAAGTTTCAAACTCTGGCTACTAATGGTAAGAGGACCAAACTCGAGTCTTTC AGTGCGTTTCTATGGAAACTCTTGGCTAAACACGCTGCAACAGAGTCGGTACTCCCGACTAAAACCTCGAAACTTGGCAT CGTTGTTGATGGAAGGAAGAAACTTATGGAGCAAGAGAACTGCAATTACTTTGGGAACGTCCTCTCAGTTCCATTTGGAG AGCGGAGAATCGATGACTTGATCCACAAGCCATTGTCTTGGGTGACAGACGAGGTTCACAAGTTACTGGAGAGTACAATG ACCAAAGACCATTTCTTGAACTTGATCGATTGGGTTGAGACTAGCCGTCCGATACCAGTTATATCAAGGATCTACAGTAC AGGATCCAACGATGGACCCGCTTTCGTTGTTTCGTCTGGAAAGAGTTTTCCAGTGGCCCGGATTGATTTCGGGTGGGGCT CGCCTGTTTTTGGATCGTACCATCTCCCACCCGGGAGCAGAGCTGGTTACGTAATGACAATGCCGAGTCCGGTGGAAAAC GGTGGCTCCGGAGATTGGACGGTTTACTTGCATCTTACGAAAGGACAGTTGAGGTTTATTGAACAAGAGGCTTCTCATGT GTTTAATCCTGTTGACAACGACTATCTCAAGATTTAAAGCATTATCAAACTTTGGTTATATTATTATGCAAATAAAGGCT TATATAAATTTGGCTTTTATAGTTGTAAATTTCAGTTTAAGATCAATATTTTGATT ...................................................N............................ ................................................................................ ...............................N..N............................................. ................................................................................ ................................................................................ .........................................N..............N................i...... ................................................................................ .............................................................N.................. ................N.........N.................................i................... ................N............................................................... .............N.................................................................. ..................i................................N............................ ...........i.................................................................... ........N................N...................................................... ............N................................................................i.. ................................................................................ ...........N.................................................................... .....................................................N.....i.................... ............................................................................N... .................................................................N.............. ........................................................ Pos Score Pred ------------------------ 52 0.329 - 192 0.419 - 195 0.429 - 442 0.283 - 457 0.437 - 474 0.778 Yes 622 0.139 - 657 0.389 - 667 0.208 - 701 0.780 Yes 722 0.278 - 748 0.144 - 759 0.232 - 765 0.328 - 817 0.201 - 894 0.403 - 979 0.530 Yes 1012 0.339 - 1052 0.543 Yes 1129 0.391 - 1146 0.448 - 1213 0.146 - 1278 0.730 Yes 1372 0.481 - 1494 0.316 - 1500 0.658 Yes 1597 0.283 - 1666 0.056 -

Explain the output -- Processing locus 296: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATTGGAAGAATTAATTTGTTAACTTAAACATGCCAAATTGAAAAACTATAATGTTGTTTTTAGTACATAAATATCCCTA CAGAAAATCCACATAATTTGAAAAGAAATGAAAAAAATAAAAAACGAACCCGGAAGTTTGGACCTTGGAGGCGGAGCCAT TTATAAACAACACTCGAGAAAACGCTTGAGAAGACGTATTCAACGTTGGGTTGGTTCCATGGATCCTCCCACCCAAAGCC ATAACTCGGTCCGTACTGTTCCAGGCTCGAATCATAGCCACACCGATTTGATTATCTCATCGTTCCTTTCGTTTCCAGAT TCCTCTCCGATCTCCATTAGCAATTCCTTCGATCGAGTCCTTGATAGAGCTCTCGCCTCCGCTTCCGCTGATGAATCCGT TCAGGATCGTCTCGTCGATCGTACTCTCGAACTCGCGTCGCTTCTTCTCGATTCCACTAGGCGTTGCTTCCGTAAGCGAG CTTCTGTTCACAATTCCAATTCCTGGTCCCTCCCTCCAGAACTTACCATTAAGGTCTTTTCGATGCTTGATACAAAGTCT ATGATGCAAGCAGCTGTGTGCTGCACCATGTTCAATAAATGTGCAATGGACCGTTTATGTTACTCCCACATCGACCTGAC AACGTCTGCTAGATATGCTGATAAAGGAGTTGTGTCTACTATGATCAATCGGGCTGGTAAAGAACTCAGATCCCTGAAAC TTGGTCGTGTTGTTCGTACAGCTGGATCTGATTCTGCTGCACCTTTGCTTAGCGGATCTTGTCTTTCCCCACTAGCATAT AATCATGGTTTTCTTGGGAGTCGCTTGAGAAGCCTACGGCTTTACAATCTCAGACCTATAAAATACAGGTCCTTATGCGA TGCGTTGTCAGTTTGTCCAAATATCACTGATCTGAGGATTGTGGGATTGTATAACTTAACCGAGGAGCTATTTAATTCAT TGACAAAAAAATGCCGCTTAATAGAGCACCTGTTCTTAGAGACCTACGGTTATCCACGTACGTTAGAGAGTAAAGCAGGA TCATCCTTAGTAGAGTTTGTGACCAATTGCCCCAACTTAACGTCCCTGACGCTCATACGTTTTGGACTAACCGATGACTG GGCCCGAAATCTTGCTGAGAGTTGTCGTAAACTGAAGTACCTGAATTTGTCCCGATCACCTACAATCAAGGGTCGTTTTT TGAGGGAGTTGGGACTTAGCTGCAAAGAAAATCTACTCAAGACTCTAATATTGCGCAGCTGCCCAAAACTACAGGAGAAA GAAGTATTGGAATTCTGTAACTCACTACTCACAGGGAACTTCAAATCCATTCGGCAAATTGATGTTTCAAGTAACAGTGG ATTAGCTTCTTCAGACAGGGGCAAGAGATGTAACAAACCAAACTTTCCTCTGGAGAGGCTGAAAGAAGAAAGATCAGATG TCACATTTGTGGCGGATTTTCCATCAACAAGTTCAGGAAAGCGTTATGGTGTGTGCGATGAAGAAGAGCTGAGGCTGATA GAGATGATGGAAGCTGAGGACGATGAAGTGGACGAAGAAGATGACTCGGATGACGACACTGACGATGTATCGGATGAAGA TGAGTCAGAAAATGATGATGACATGGGAATGGGATTTGATGTCGATTATTTGTTGTGATTAGTGTTTGGTAATCGTTAGT ATTTCTTATTGAACTAGTCTAGTCCAAAGGGAAGTTCTTTGTTTCTTTCCTTCTTATTGGGAAATACAAACATACCA ..............................i....................i............................ ...........................N.................................................... ..........................................................N..................... ................................................................................ ......................................................................i......... ................................................................................ ..............................................................i................. N..i.......................N..........i......i..........N....................... ..............N.........................N....................................... ................................................................................ ....N.....................................................................i....N ................................................................................ ..........i..................................................................... .........................................................................i...... ................................................................................ ................................................................................ .............................................................N.................. ...........................N.................................................i.. .............................................N...........N...................... ...i..i...............N.................N........N..............N........N.....N ...........N..N..N....N.....N.........N......................................... ............................................................................. Pos Score Pred ------------------------ 31 0.671 Yes 52 0.767 Yes 108 0.333 - 219 0.372 - 391 0.581 Yes 543 0.600 Yes 561 0.213 - 564 0.627 Yes 588 0.359 - 599 0.527 Yes 606 0.825 Yes 617 0.296 - 655 0.235 - 681 0.386 - 805 0.398 - 875 0.560 Yes 880 0.360 - 971 0.682 Yes 1114 0.774 Yes 1342 0.487 - 1388 0.412 - 1438 0.676 Yes 1486 0.352 - 1498 0.130 - 1524 0.859 Yes 1527 0.877 Yes 1543 0.462 - 1561 0.265 - 1570 0.064 - 1585 0.103 - 1594 0.086 - 1600 0.061 - 1612 0.159 - 1615 0.106 - 1618 0.183 - 1623 0.304 - 1629 0.263 - 1639 0.118 -

Explain the output -- Processing locus 297: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAGCCAAAGCTTTTTATGACCACAAGGCGCTGTCTGATTGGCTCAACTATAAAGTTAATTAACAAAAAAAATTATGATAC CAATTATTACTAGCTCTATAAATACGTAGCCACTCTCTAGGTTGCATGACTCACAAATCAACCATTTCTCCTTTCTTCTT ATTTCTGAGCAAAGTTTTTTCTTCCTTTCATATTGTGAGGATGTCCAAAACCAATATGAAATTTTGCAATAGTTATTTTC TGGTCGATCCGACCAAAGCAAGTTTTCTTGATCTCCTTTTGCTTTTGTTTTCCTCCAACCTGACCAGTGCAAGATTCATC GATTCTCCTCCGGATACGCTTAAAGGCTTCCGGAGAAGTTTCGCGAGTCGATGGATCCTTGCGTTGGCCATCTTCCTTCA AAAAGTATTAATGCTCTTAAGTAAACCTTTTGCATTCATTGGTCAAAAGCTCACATATTGGCTCAACCTTCTTACGGCAA ATGGAGGCTTCTTCAACTTGATACTTAACCTTATGTCAGGAAAGCTGGTCAAGCCTGACAAATCTTCGGCGACGTATACT TCTTTTATAGGATGCTCAGATCGACGAATAGAGCTTGACGAGAAGATAAATGTTGGTAGCATCGAATACAAATCGATGTT GTCAATAATGGCTTCTAAGATCTCTTATGAGAGCAAACCTTACATCACTTCCGTCGTCAAAAACACTTGGAAGATGGACT TGGTGGGTAACTACGACTTTTACAACGCTTTCCAAGAAAGTAAATTGACGCAAGCCTTCGTGTTCAAGACTTCGAGCACC AATCCAGACCTCATCGTCGTTAGCTTCAGAGGAACCGAACCTTTCGAGGCTGCCGATTGGTGCACTGATCTCGACCTCTC TTGGTACGAGATGAAGAACGTTGGCAAAGTCCACGCCGGGTTCTCAAGAGCTTTAGGTCTCCAAAAAGATGGATGGCCCA AGGAAAATATTAGTCTACTACACCAATATGCTTACTACACCATCAGACAGATGCTTAGAGACAAGCTTGGCAGAAACAAG AACCTTAAATATATTCTAACAGGTCACAGCCTTGGCGGAGCACTAGCTGCTCTTTTCCCGGCGATTCTGGCCATTCACGG TGAGGATGAGCTGCTAGATAAGCTAGAGGGAATCTATACGTTTGGACAGCCACGTGTAGGAGATGAGGACTTTGGTGAGT TTATGAAGGGTGTGGTGAAAAAGCATGGAATAGAGTATGAGAGATTCGTCTATAACAATGATGTTGTGCCTAGAGTGCCC TTTGATGACAAGTACTTATTCTCATACAAGCACTATGGACCTTGCAATTCCTTCAACAGTCTCTACAAAGGAAAGGTAAG AGAAGATGCACCAAATGCGAACTACTTCAACTTGTTGTGGTTAATACCGCAGCTGTTGACTGGTTTGTGGGAGTTTATAA GGAGTTTCATATTACAGTTTTGGAAAGGCGACGAGTACAAAGAGAATTGGTTAATGAGATTTGTTAGGGTTGTGGGAATA GTGTTCCCTGGTGGCTCTAACCATTTCCCATTTGATTACGTCAACTCTACTCGCCTAGGAGGCTTGGTTCGACCTCCTCC TACTACTACTCCTGAGGATAAACTTGCCCTCATTGCTTGAACTCTCATTATTTAATAATCATATCCACTTTATCTGCCCT AATGACGGTTAAATAATTGCACTTGAGCTTTAATGTATTCTCTGTGTTATGTGTTTTTG ...............i.........................................................N...... .............................................N.................................. ........................................i..............i........................ ................................................................................ ..................................................N............................. ..........N..................................................................... N...............................N............................................... ...........N.....................................N.........................N.... .......i..................N..............................................N...... ................................................................................ ................................................................................ ..........i.........................................................i...N....... ...........................N......................i............................. ................................................................................ .....N........................................................N................. ..i.....................N...........N....................N..i................... ....N.............................N............................................. .....i........N................................................................. .....................................................i.......................... ................................................................................ ................................................................................ Pos Score Pred ------------------------ 16 0.676 Yes 74 0.295 - 126 0.442 - 201 0.560 Yes 216 0.509 Yes 371 0.332 - 411 0.447 - 481 0.352 - 513 0.354 - 572 0.214 - 610 0.275 - 636 0.498 - 648 0.518 Yes 667 0.260 - 714 0.472 - 891 0.609 Yes 949 0.515 Yes 953 0.478 - 988 0.297 - 1011 0.525 Yes 1126 0.225 - 1183 0.138 - 1203 0.512 Yes 1225 0.367 - 1237 0.236 - 1258 0.458 - 1261 0.576 Yes 1285 0.319 - 1315 0.478 - 1366 0.535 Yes 1375 0.321 - 1494 0.519 Yes 1682 0.130 - 1713 0.049 - 1729 0.049 -

Explain the output -- Processing locus 298: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTCAAAATTCAGAATCTGTAGCAAATTTGAGTAATTTATAAGGATTGTTGCAGATAACGAAAACCTTATTGGGATGTG GGAGAGTTCTAATCCGTTTAAAATCTGGATGACGTTATGCAGAAGCGGAGAATCATCACTGTGAACCCCTCCGCGTCTAT TGAGATGAGTCAGTACGAGAATAATGAAGTTCCTTACTCAAGTGTCGGTGCTGATGAGGTTCCATCATCACCGCCTAAAG CTACCGATAAGATTCGGAAAGTTTCCATGTTGCCACTTGTTTTCCTCATATTCTATGAAGTCTCAGGAGGTCCTTTTGGT GTAGAGGATAGTGTGAATGCAGCAGGACCATTATTAGCTCTTCTAGGGTTTGTGATCTTTCCTTTCATTTGGAGTATCCC TGAGGCTTTGATTACTGCGGAGATGGGAACAATGTATCCCGAGAATGGTGGTTATGTTGTGTGGGTTTCCTCTGCTTTGG GACCTTTTTGGGGGTTTCAGCAAGGTTGGATGAAATGGCTTAGTGGTGTTATTGATAACGCTTTGTATCCTGTTCTGTTT CTTGACTATTTGAAGTCTGGAGTCCCGGCTTTAGGTAGCGGTTTACCGAGAGTTGCATCGATCTTGGTGCTGACTATTTT GTTGACGTATCTGAACTACAGGGGACTAACTATAGTTGGTTGGGTTGCTGTGCTTATGGGAGTTTTCTCCATCCTTCCGT TTGCTGTGATGGGTTTGATTTCGATTCCGCAGCTAGAGCCTTCGAGATGGCTTGTGATGGACTTAGGGAATGTGAACTGG AATTTGTATCTTAACACGCTTTTCTGGAATCTAAACTATTGGGACTCGATTAGTACACTAGCTGGTGAAGTTGAAAACCC CAACCATACACTTCCAAAGGCTTTGTTTTATGGTGTGATCTTAGTTGCTTGTTCTTACATCTTCCCTCTTTTGGCTGGAA TCGGGGCAATCCCGCTAGAGCGTGAGAAATGGACAGATGGGTATTTTTCTGATGTGGCCAAAGCTCTTGGTGGAGCGTGG TTGAGATGGTGGGTTCAAGCGGCTGCAGCTACATCAAACATGGGAATGTTTATAGCCGAGATGAGTAGTGACTCTTTTCA GCTTCTCGGAATGGCTGAGCGCGGTATGCTTCCCGAGTTCTTTGCCAAAAGGTCACGTTACGGGACACCTTTACTAGGGA TTCTGTTTTCAGCGTCAGGTGTTGTTCTCTTGTCTTGGCTAAGCTTTCAAGAGATTGTAGCTGCAGAGAACTTACTCTAC TGCGTTGGTATGATCTTAGAGTTTATAGCGTTTGTTAGAATGAGAATGAAACACCCTGCTGCATCAAGACCGTACAAGAT ACCTATTGGAACCACAGGTTCGATCCTCATGTGTATTCCTCCAACCATACTGATCTGTGCTGTTGTAGCACTCTCGTCTC TTAAAGTAGCTGCAGTGAGCATTGTGATGATGATCATTGGTTTCCTGATACATCCTTTACTGAACCATATGGATCGAAAG AGATGGGTCAAATTCTCCATCAGTTCTGACTTACCGGACCTTCAACAGCAGACTCGGGAATACGAAGAAACTCTAATACG TTAAGAATGTCCTTAGTTATTTCCCTCGTCTATGTTAAGTACAGAGCTTGGAAGCTATAGCCAAAAAAGCTTAATTTCTC ATAGTCTGTACTCTGTTTTATTC ...........................................................................N.... ............................N.......i........................................... ....i..................N.............................N.......................... ..........................N...........................N......................... ................N............................................................... ......................N........N............N........N.......................... .............................i....N............................................. ................................................................................ .......................................................N........................ ........N.....................................i.........N............i.......... ................................................................................ .............................N.................................................. ............................i.......i..............N............................ .....i.................................N.....i..............N................... ..........N..............N...................................................... ................................................................................ .........N.............................N.....N.................................. ............................N................................................... ..........................N..N......................................N........... ..i............................................................................. ......N........................N................................................ ....................... Pos Score Pred ------------------------ 76 0.263 - 109 0.155 - 117 0.650 Yes 165 0.744 Yes 184 0.318 - 214 0.109 - 267 0.355 - 295 0.405 - 337 0.171 - 423 0.173 - 432 0.362 - 445 0.403 - 454 0.130 - 510 0.555 Yes 515 0.357 - 696 0.450 - 729 0.124 - 767 0.785 Yes 777 0.201 - 790 0.582 Yes 910 0.191 - 989 0.761 Yes 997 0.649 Yes 1012 0.319 - 1046 0.608 Yes 1080 0.375 - 1086 0.660 Yes 1101 0.480 - 1131 0.496 - 1146 0.160 - 1290 0.379 - 1320 0.355 - 1326 0.205 - 1389 0.300 - 1467 0.400 - 1470 0.408 - 1509 0.208 - 1523 0.637 Yes 1607 0.279 - 1632 0.128 -

Explain the output -- Processing locus 299: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGAGCCATACTACTCTGACTTGATTTTTGGAAATAAAAATCACAAGAAGAAGTTTCTAACCACCCTACGTATGTATATG ATTATAAAATTGAGATTATGAAACATCATGACTTTTATTCCCCAATCAAAAAATTCCTCTGTAAGACACCACACCAAAAT ATTTATTAATAATATATTATTTGCGTGTGACTTTCTGACATTTTTCTCTCTAAACTTTGACATGGATCATCAAATCAAGA ACACCATGAAGAACAACAACAGTTCATCAGTTTCAATCGAGAATCATCCATGGAAGAAAAAACCAACCACTCTTTTACTT TTCCTTTCTCTTCTCTCCATCTCTCTGCTTCTCCTTCGTCTCTCCCAAGACAAAATCATACTCATCACCACCACCAAAAC CACAGCCACCACTGGAACCGATCATCAACGCAGTCACAAATCTTCTGAGGACGATACGTGTCTCGGACGCTACATATATA TCCACAATCTACCTTCTCGATTCAATCTTGAAATCATCAAAGATTGTAAGTCTATTACAAGACCAAAGGACAAAATCAGT ATGTGTAAGTATCTCGACAACTCCGGATTCGGACCTTTGATCGGCGGTAAAAGTTCTGACTACTCTCCGAGCTGGTACGC TACTAATCAGTTCATGCTCGAAGTGATTTTTCACGAGAAAATGAAGAGTTACGAGTGCTTGACGAGAAATTCTTCACTGG CTTCAGCTATTTACGTACCTTATTATGCTGGCCTCGATTTCCGGCGACATTTACGGCGGCGTAACGTTGCTGCGAGAGAC GCCGCCGGGAAGGAACTTGTTAAATGGCTCAAGAAGCAACCTCAGTGGAAAGATATGTCAGGTAAAAACCATTTCCTTGT AACCGGTCGGATTTCACGAGATTTCCGGCGAAACTCCGGCAGTAGGTCAGCATGGGGAACCAACTTCATGCTCTTATCTG AGTCGCTAAACCTCACTTTCCTCAGCATTGAACGAAGTCTAACGAGCCACAACGAGTTCGCAATTCCTTATCCTACTTAC TTTCACCCTACGTCAACCCCCGAGATCCTTCAGTGGCAAGAAAAGATCAGGCTTACAAACCGGACGGTCCTCTTCTCATT TGCCGGAGCTCAAAGACCAAGCAGAAACCAGAACGGTGTGGTTCGCACTGAAGTTATAAAACAATGCAAGAGTTCATCTA AAACGTGTAGGTTTCTTGACTGTGACGTCAATGCAAATAGCTGTGATGATCCGATCAGTCTGATGAAACTTTTCGAGAGT TCGACTTTCTGCTTACAACCGCCGGGTGATTCGTTGACCAGAAAATCGGTATTTGATTCAATCTTGGCTGGTTGTATACC GGTTTTCTTTAACCAAGGAAGTGCATACAAGCAATATTTATGGCACATACCTAAGAATAGTAGCAAATATTCTGTTTATA TAACGGTTAAGGAGCTGAGAACCGGTGGAAAGAACAAAATCGAAGAGATTTTGCGTGGAATACCAAATGAGAGAGTGGTT GGTATGAGAGAAAACGTAATAAGATTGATTCCGAAGATTGTGTATGCTAAACCAAACCGGAACAAACCGGATGGAGAGAT TCTTGAAGATTCTTTTGATGTTGCTGTGAAGGGAGTGCTAGAGAGAATAGAAGGGATAAGGAGAAACGAATTCAAAACTG ATTAGCAAATTTTATAGTTAGCCTAAACAAATGAAGATGTCCTACATTTGTTATTGTATTTAATTGTTCATAGGTCTTGT ACAAAGATAAGTCTATCAAACATG .......................................................................N.....N.. .................N.........N.................................................... .............................................................N.................. .....N...........................................i.............................. ................................................................................ ................................................................................ ................................................................................ i............................................................................... .............N..........................N....................................... ........................N....................................................... .......................i..............................i......................... ...................................................N...............N............ ................................................................................ ................................................................................ ...............................................................i................ ..............................N..............N................N................. ................................................................................ .......................................N........................................ ..................................................................N............. ...i.......................................N..........................N......... .................N.............................................................. ..............................N.....N........................................... .....................N.. Pos Score Pred ------------------------ 72 0.120 - 78 0.057 - 98 0.312 - 108 0.155 - 222 0.312 - 246 0.266 - 290 0.736 Yes 561 0.680 Yes 654 0.321 - 681 0.456 - 745 0.346 - 824 0.553 Yes 855 0.654 Yes 932 0.422 - 948 0.332 - 1184 0.612 Yes 1231 0.088 - 1246 0.223 - 1263 0.184 - 1400 0.458 - 1507 0.221 - 1524 0.524 Yes 1564 0.174 - 1591 0.358 - 1618 0.105 - 1711 0.061 - 1717 0.145 - 1782 0.049 -

Explain the output -- Processing locus 300: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAAAGAACTATGAGAGTTTCAAAAAGAAAATCAAAAGCCAAAACAAAGCTTTTCTTGCATGACTCAATAAACCTACACT ACACCATACTCTTACTTATAAACCTCATCTCCAATGCCACACCATTCCATCTTAAAATCACATTCTGATCATCACCAACA CATTGCAAACCAAACCAGACACAAACACAAAGACATGTCTCTTCTTGCTTTCTTCCTCTTCACCATCCTTGTCTTTTCAA GTTCATGTTGTTCCGCAACTCGGTTCCAAGGGCACAGGTACATGCAGAGGAAAACAATGCTAGATTTGGCTAGCAAGATT GGTATCAACTATGGAAGAAGAGGAAACAACCTCCCATCTCCATATCAATCCATCAACTTCATCAAATCTATCAAAGCTGG TCATGTCAAGCTCTATGACGCCGATCCAGAGAGTCTCACACTCCTCTCTCAAACCAATCTCTACGTCACCATAACCGTCC CTAACCACCAAATCACCGCCCTCAGCTCTAACCAAACCATAGCTGACGAATGGGTCAGAACTAACATCCTCCCTTACTAT CCACAAACACAAATCCGTTTTGTCCTTGTCGGAAACGAAATCCTCAGCTACAATTCTGGGAATGTCTCTGTGAATCTTGT ACCGGCGATGCGCAAAATCGTTAACTCACTCAGATTACATGGGATTCACAACATCAAAGTTGGGACACCTCTAGCTATGG ATTCTCTCCGGTCGTCGTTTCCTCCATCGAACGGAACATTCCGGGAAGAAATCACCGGACCGGTGATGTTACCGTTGCTG AAGTTTCTCAACGGAACAAACTCTTACTTCTTCCTTAATGTTCATCCTTACTTCCGTTGGTCAAGAAACCCCATGAACAC CAGTTTGGATTTTGCTCTGTTCCAAGGACACTCAACCTATACCGATCCTCAAACCGGTTTGGTTTACCGTAATCTTCTAG ACCAAATGTTGGATTCGGTTCTCTTCGCCATGACCAAACTCGGTTATCCACATATGCGCCTCGCGATCTCTGAAACCGGA TGGCCTAATTTCGGTGACATCGACGAAACCGGAGCCAACATTCTCAACGCAGCTACCTATAACCGTAATCTGATCAAGAA GATGAGCGCAAGTCCTCCAATCGGTACACCATCAAGACCCGGTTTACCAATACCGACATTTGTTTTCTCCTTATTCAACG AAAACCAGAAATCCGGTTCGGGGACACAGAGACATTGGGGAATCTTGCATCCCGACGGTTCACCAATCTACGACGTAGAT TTCACCGGTCAAACACCCTTAACCGGTTTCAACCCGTTACCTAAACCGACGAACAACGTTCCTTACAAAGGTCAAGTGTG GTGCGTACCAGTCGAAGGAGCCAACGAGACTGAGCTTGAAGAAACATTGAGGATGGCTTGTGCCCAAAGCAACACCACTT GTGCAGCTTTAGCTCCTGGGAGAGAATGTTACGAACCAGTCTCCATTTATTGGCATGCAAGCTACGCGCTTAATTCGTAC TGGGCTCAGTTTCGTAACCAAAGCATTCAATGTTTCTTCAATGGATTGGCTCATGAGACAACAACCAACCCTGGAAATGA TCGTTGCAAGTTTCCGAGCGTTACTCTGTGAGGAGGACTTGAGGAAGAAGACACATGATTAAAGCTGGATTATTCGTATA ACTCAATATTGTTCCTTATCTTTTTTTTATTATACCTTTTCTGCTTCAAG ..........N................................................N.................... .................................N.............................................. ..................................i............................................. ....i....................................N..............i....................... ..........i..................................................................... ..i...........N................................................................. .................................................N.............................. .............................................................N.................. .......N..............................N.....................................N... .................................................................N.............. .....................................N..................................i....... ................................................................................ .....i.......................i.......................i.........................i ................................................................................ ................................................................................ ................................................................................ ....................................................i........................... .........................N............................N......................... .............................N..........N...........N.......................N... ......................................................N......................... .................................................. Pos Score Pred ------------------------ 11 0.365 - 60 0.271 - 114 0.353 - 195 0.621 Yes 245 0.551 Yes 282 0.259 - 297 0.790 Yes 331 0.827 Yes 403 0.542 Yes 415 0.166 - 530 0.465 - 622 0.273 - 648 0.364 - 679 0.316 - 717 0.493 - 786 0.156 - 838 0.213 - 873 0.790 Yes 966 0.852 Yes 990 0.694 Yes 1014 0.688 Yes 1040 0.646 Yes 1122 0.643 Yes 1413 0.805 Yes 1466 0.121 - 1495 0.451 - 1550 0.298 - 1561 0.467 - 1573 0.195 - 1597 0.327 - 1655 0.084 -

Explain the output -- Processing locus 301: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAGAGTTAACAATCACTAAGCATTCCAATAATATATTTTTCAATGGAATGGAATTACCATTGTGACATAACTTTTTGTT TTTTGTCAAACTGTGACATAACATAAAACCATTAAAGAAAACATGTGTCCCTCCTTTAATAAGAAGCACATTGTGACAAA CAGAGCATTCTCTCTCTCTCACACTCTCCTCTGTCTCGCTACCTCTGTCTCTTTCTCGAGCACTCACTGTTTTATTTACC AGAAATTGTGTTGTATCATTACATGCATGCATAAAATGATGGTGGTGGAGAAAGAAAGAAGCATAGAAGAAAGGCTTTTG CAGCTCAAGAACCAAAACGACGATTCAGAATGTCGTATTACTGCTTGTGTTATCCTTAGTACTTTCATCGCTGTGTGTGG TTCTTTCTCCTTTGGTGTCTCTCTTGGTTATACTTCAGGTGCAGAGATAGGAATTATGAAGGACTTGGATCTTTCTATCG CGCAATTTTCAGCATTTGCTTCATTATCCACTTTGGGAGCTGCAATTGGAGCTTTGTTTAGCGGTAAAATGGCGATCATC CTTGGCAGAAGAAAAACAATGTGGGTGTCCGATCTCCTCTGTATCATTGGTTGGTTCTCCATCGCTTTCGCTAAGGATGT GATGTGGTTGAACTTCGGAAGAATCTCCTCAGGGATAGGACTCGGCTTAATTAGCTATGTGGTACCTGTCTATATAGCAG AAATTTCACCAAAACATGTTCGTGGTACATTTACCTTTACAAATCAGCTCCTTCAAAACTCTGGACTCGCAATGGTTTAT TTTTCTGGGAATTTTCTCAACTGGAGGATATTGGCTTTATTAGGTGCTTTACCATGCTTCATTCAAGTAATCGGTTTATT CTTCGTACCAGAGTCCCCAAGATGGCTGGCAAAAGTTGGCTCCGATAAAGAACTAGAAAATTCACTTCTTCGTCTTAGGG GGGGAAATGCTGATATTTCACGTGAAGCCTCAGACATTGAAGTTATGACCAAAATGGTAGAAAATGATTCAAAGTCGAGT TTTTGTGATTTGTTCCAGAGAAAATATAGATACACTCTCGTGGTAGGAATCGGGCTAATGTTGATACAACAATTTTCAGG AAGTTCTGCAGTGCTCTCATATGCCAGTACCATTTTACGGAAAGCAGGTTTTTCGGTGACCATTGGATCGACGCTACTAG GTCTCTTCATGATACCAAAAGCCATGATTGGTGTCATTCTTGTCGATAAATGGGGCAGACGGCCTCTCTTGTTGACTTCT GTATCTGGGATGTGCATAACTTCCATGCTTATTGGAGTCGCCTTCACATTACAGAAAATGCAATTGCTACCGGAGCTAAC ACCAGTATTCACGTTTATATGCGTTACGCTGTATATTGGAACTTATGCTATAGGCTTGGGAGGCCTTCCTTGGGTAATTA TGTCTGAGATATTTCCAATGAATATAAAAGTAACTGCAGGAAGTATAGTAACGTTAGTATCATGGTCAAGTAGTTCGATC GTCACTTATGCTTTCAACTTTCTGCTCGAGTGGAGCACTCAAGGAACATTTTACGTATTTGGAGCAGTCGGAGGACTAGC ATTGCTTTTTATTTGGTTGCTCGTTCCGGAAACGAAAGGATTATCACTTGAAGAAATACAAGCTTCGCTTATTCGTGAGC CCGATAGAATAAATCAAAGTTAGATTGAAACGTTTAAGTTTTATTTTAATAGATAAATAATTAAAATCAATTTATGATAC TGTGCTAAGAAAGTAATTTTTTTTTTCATTATGTAATCACTA ...........................................N....N............................... ..........................................N..................................... ................................................................................ ......................N...N........i..i......................................... .............................N.................................................. .......................................................i........................ ....................................................................i........... ..................i.........................................................N... ...............N.......................................................i........ .....................................................N.......................... .....................N.......................................................... ......N.....................................N........N.........N................ .........................................................N...................... ....................N........................................................... ........N..............N.........................i.............................. .........N..............N................................N...................... ..................N.........................N..................................N .................N...........................................N.................. .......N........................................................................ ................................................................................ .........................................................................N...... ..............................N........... Pos Score Pred ------------------------ 44 0.242 - 49 0.185 - 123 0.498 - 263 0.380 - 267 0.202 - 276 0.676 Yes 279 0.591 Yes 350 0.239 - 456 0.694 Yes 549 0.637 Yes 579 0.653 Yes 637 0.238 - 642 0.421 - 697 0.332 - 736 0.356 - 792 0.535 Yes 854 0.364 - 902 0.468 - 967 0.166 - 1005 0.180 - 1014 0.290 - 1024 0.251 - 1098 0.494 - 1141 0.157 - 1209 0.190 - 1224 0.496 - 1250 0.840 Yes 1290 0.164 - 1305 0.112 - 1338 0.202 - 1379 0.345 - 1405 0.139 - 1440 0.289 - 1458 0.488 - 1502 0.257 - 1528 0.230 - 1754 0.093 - 1791 0.059 -

Explain the output -- Processing locus 302: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAAATATGCAATTTATTTTATTATCAGGCTCGAAACAGAAGTTCTCATAAATATCGGGGCGTTAAATTTCGTGAAAGGT GACAATAGTGCAATTTATTGAAACATAGGGGTCGAAAAAGGAGATTCTCAAAACTATCGGGTCATTTCCAAAGTTCCAAA AAGTTTGAAATTTTGTGCGGGCATTTTTTATCCGAAGATGCAAAGGAAAAAAACAAAAAAGGAAGGATTTTTATTTTTTT ACCGTGATAGTAACGAACGAGACAGTGACGAGACAACAATCATCATTCCCAATAATTCTTCTCACCAAGAATCTAAAATC CTTCTCCGATTTCCTCTCATGGCGGCGGAATCAACCAGAAACTCTTCTCCGCCGTCTACTTCTCAGTCTTCAAGCCCTAT AATCAATTTACCCGATGACCATCTCTTAACCATCTTGCTTCTTCTTCCCGTAGATTCAATCCTCTCCTTCTCAATGACCT GCAAGCGATACAAGTCTCTAGCTTGCTCCGACAGTCTCTGGGAAGCTCTGTGTGAACGAGAATGGGGACCAACATCTGTA GATGCTTTAAAGTTGTCATCTTTACGAGATGGGTTTTCATGGATGTTGATGTTTCAGCGAGTTTATAAGATGGATTCTGT TTGCTGTCACAAGATCTCAGACCCAGATGATGATGATGAAGAATCTTCTTCGTTTCCGATCCCTAGAGCTTCTCATTCTC TCAACTTCGTTAATGATCATCTTGTTCTCTTTGGTGGTGGTTGTCAAGGAGGACGCCATCTTGATGATACATGGACATCA TATGTTGATAAGAGCAATCAAAGCATATTGAAATGGAAGAAAGTTAAATCAGGGACTCCAAGTGGGAGATTCGGACACAC CTGCATCGTTATAGGCGAGTATCTACTCTTATTTGGAGGTATAAATGACCGTGGAGAGAGGCTAAATGACACATGGATTG GTCAAGTCTTTTGCCACGAGGGACTCTCTTGGAAACTCCTCAATGTTGGGTCTTTGCAGCGGCCTCGTCCTCCTCCTCGT GGTGCTCATTCCGCCTGTTGTATTGCTGAGAAGAAGATGGTTGTACATGGAGGAATCGGGCTGAATGGAGTCAGGCTTGG AGATACATGGATTCTTGAACTTTCTGAAGATTTCAGCTCTGGGACATGGCACATGGTGGAGTCTCCACAATTACCGCCAC CGCGCTCAGGGCATACTTTGACCTGCATTAGAGAAAACCAAGTGGTTCTATTTGGAGGCAGAGGATTAGGATATGATGTG CTTGATGATGTTTGGATCTTGGATATTCAAGAACCGTGTGAAGAAAAATGGATACAGATATTCTACGATTTCCAGGATGT TCCCGAATACGCATCGTTGCCTCGGGTTGGTCACTCGGCGACTCTTGTATTGGGAGGTCGAATCTTGATTTATGGCGGTG AAGATTCTTATAGGCATAGGAAAGATGATTTCTGGGTGTTGGATGTTAAAACCATTCCTTCATCAGGTTTGAAACCACAA GGGCTTAGCTTAAACGGATCATCAGTGTGGAAAAAGCTTGATCGAATCTCATATGGACCAAAAAGTCGGTCTTTTCACCG TGCCTGCGCTGATTGTTCAGGGAGATTTTTATATGTGTTTGGCGGAATGGTTGATGGTCTTCTTCAGCCTGCAGCATCGT CTGGTCTAAGGTTTGATGGAGAGCTTTTCATGGTGGAGCTTGTTCTTGGCTTCTCTGACCTAGACCATCAACAAAGACCC GGAAAAAGCATGTAATAGTGGTGGTTCCTTCTTTTGTAAATTAGCAGATTTTGTTTAAAAGCATATCGAGAAACCAGAAA TGGAACCCACATGATCAAATCTCTGATTACAGAG ......N......................................................................... ................................................................................ .....................................N.......................................... ................................................................................ ..................i............................................................. ..............N..........................................................N...... .............................................................N.................. ..........................N..N..N..N............................................ ............N..................................................N......N......... ............................................i....................N......i....... ..........................................i..................................... ....................................i.........N.................i............... ......N......................................i......N........................... ........................................................................N..N.... ....N..N.......................................i............................N... .......................................................................N........ ........................i.................N..................................... ....................................................N........................... ................................N.............i......N.......................... ...............i.............N.................................................. .........N.....................................................................N ..........N....................... Pos Score Pred ------------------------ 7 0.181 - 198 0.390 - 339 0.620 Yes 415 0.399 - 474 0.362 - 542 0.447 - 562 0.281 - 589 0.277 - 599 0.222 - 603 0.338 - 609 0.443 - 630 0.465 - 667 0.143 - 670 0.300 - 673 0.401 - 676 0.408 - 733 0.210 - 784 0.350 - 791 0.428 - 802 0.194 - 833 0.624 Yes 925 0.564 Yes 946 0.416 - 953 0.625 Yes 1003 0.665 Yes 1077 0.719 Yes 1087 0.251 - 1105 0.679 Yes 1127 0.389 - 1166 0.705 Yes 1173 0.409 - 1273 0.315 - 1276 0.305 - 1285 0.273 - 1288 0.225 - 1328 0.583 Yes 1357 0.213 - 1432 0.435 - 1465 0.540 Yes 1483 0.109 - 1573 0.458 - 1633 0.333 - 1647 0.825 Yes 1654 0.345 - 1696 0.533 Yes 1710 0.160 - 1770 0.323 - 1840 0.093 - 1851 0.043 -

Explain the output -- Processing locus 303: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCAATTTTTCTTACCATAACTTTGTCCTTGCATGTAACTTTTCCAATTAAATACAAGTCTGTCTTTTATTAACCCCCGA GAATTTACTAAATGATATTGACATAATCTATTGGACGGAATGAGTTGTCAAAAAACGAGAGCTGTCTTTTCCTGCTCCAC TGTCCTCTTATATATCCCACAACAATCTTTGCTACATTTTCATGCATTTTTAATCACTCCTCTTTCTAACTCCAATAAAA ACACAATAATGGATCTTCTTGTTATGCTCCTCTCTCTTCTCGTCTCTTATCTCATCTTCAAGATCTGGAAACGCATAGAC TCCAAGAGAGACCAAAACTGCTACATCTTGGACTACCAATGTCACAAACCTTCCGACGATCGTATGGTGAACACTCAATT CAGCGGTGATATCATCCTTCGAAACAAACACCTTCGGCTCAACGAGTACAAGTTCCTCCTCAAAGCCATCGTTAGTTCCG GTATCGGAGAGCAAACTTACGCACCAAGGCTCTTCTTTGAGGGCCGTGAACAGCGTCCTACGCTACAGGATGGGCTCTCG GAGATGGAAGAGTTTTACATAGACACCATCGAGAAAGTCCTTAAGAGAAACAAAATTTCACCTTCGGAGATTGACATCCT CGTAGTCAACGTCTCAATGCTCAATTCAACGCCTTCACTATCTGCAAGAATCATTAACCATTACAAAATGAGAGAAGACA TAAAAGTCTTCAACTTGACGGCGATGGGATGTAGCGCGAGTGTTATCTCCATAGATATTGTCAAAAACATCTTCAAAACA TACAAGAACAAGCTTGCTCTTGTCGTGACCTCAGAGTCACTTAGTCCTAACTGGTACAGCGGAAACAACCGGTCCATGAT TCTTGCCAACTGTTTGTTCCGCTCTGGTGGCTGCGCTGTTCTCTTGACGAACAAGCGTAGTCTGAGCAGGAGAGCCATGT TCAAGCTAAGGTGTTTGGTAAGGACGCACCATGGAGCCAGAGACGATTCATTCAACGCCTGTGTCCAAAAAGAAGACGAG CTAGGACATATCGGTGTCCACTTAGACAAGACTCTTCCAAAAGCAGCGACTCGTGCTTTCATAGACAACCTCAAAGTCAT CACTCCCAAGATTCTTCCGGTAACAGAGCTTTTAAGGTTTATGTTGTGTCTTCTTCTCAAGAAGCTTCGTAGCAGCCCTA GCAAAGGCTCGACGAACGTGACTCAGGCAGCTCCAAAGGCAGGGGTTAAAGCAGGGATTAACTTCAAGACCGGTATTGAC CATTTCTGCATTCACACTGGAGGTAAAGCGGTTATCGACGCGATCGGTTACAGCCTCGACTTAAACGAGTACGATCTTGA ACCGGCGAGGATGACGTTACACCGGTTTGGAAACACGTCAGCGAGTAGCTTGTGGTATGTTTTGGGGTATATGGAGGCAA AGAAGAGGCTGAAGAGAGGAGATAGAGTGTTCATGATCAGCTTTGGGGCAGGTTTCAAGTGCAATAGTTGCGTTTGGGAA GTTGTGAGAGATCTTAACGTTGGAGAAGCTGTAGGGAATGTGTGGAATCATTGCATTAATCAATACCCACCAAAATCGAT TTTGAATCCGTTTTTCGAGAAGTATGGTTGGATTCATGAAGAAGAAGATCCTGATACTTTCAAGATGCCCGAAGGATTTA TGTGAATGTTTTCTTGGGAATCATTGTAAACATACATATACAGTTACTTCAAACTGCAAATCCCACAAAAGGTGACTATA TAGTTTATTTTCCTCTTTCTTTCT ................................N............................................... ...........N...........................N........................................ .........................................N...................................... ........i..............N........................................................ ......................................N........................i................ ................................................................................ .....................................................................N.......... ...i............................................................................ ................N..................................................i............ .......................N....i................................................... ...........................................................................N.... ............................................................................i... ..............................i................................................. ................................................................................ ........................................N....................................... ................................................................................ ................................................................................ ..........i.............................................N.............N......... ................................N............................................... .....................................N.......................................... .......................N...........N............................N..............N .....N.......................................................................... ........................ Pos Score Pred ------------------------ 33 0.156 - 92 0.236 - 120 0.394 - 202 0.196 - 249 0.594 Yes 264 0.493 - 359 0.344 - 384 0.562 Yes 550 0.336 - 564 0.554 Yes 657 0.377 - 708 0.783 Yes 744 0.497 - 749 0.679 Yes 876 0.215 - 957 0.635 Yes 991 0.530 Yes 1161 0.226 - 1371 0.635 Yes 1417 0.263 - 1431 0.425 - 1473 0.186 - 1558 0.247 - 1624 0.230 - 1636 0.459 - 1665 0.245 - 1680 0.099 - 1686 0.131 -

Explain the output -- Processing locus 304: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCTGCGGCATCAATTTCCACCGGTAGGTTTCCGTTTACTTGTGCAATTAGGTGTGATACTGATAGGACTAAAGTTTCTG CTGTGGATAGATATGCATTGATGTATCATGTACCAAGCTGCGTTTTCCAAAAGTTCTTACATGTACCAATACATTGTAGG AATTGAAGCTGGGAATGAGAGGAAACCTGGCCTTTACTGTTGTAACTATTGCGATAAAGATCTGTCTGGTTTGGTTCGTT TCAAATGTGCTGTTTGTATGGACTTTGATCTTTGTGTGGAATGCTTTTCTGTCGGCGTTGAACTTAATCGTCACAAGAAC AGTCACCCATATCGTGTTATGGACAATTTGTCTTTTTCGCTTGTTACTTCTGATTGGAATGCCGATGAAGAGATACTCCT TCTTGAGGCCATTGCGACATACGGGTTTGGCAATTGGAAAGAAGTTGCAGACCATGTTGGTAGTAAGACAACGACAGAAT GTATTAAACACTTCAATTCTGCTTACATGCAGTCACCATGCTTTCCACTTCCGGACTTGTCCCATACTATTGGAAAGAGC AAAGATGAGCTGCTTGCTATGAGTAAAGATAGTGCAGTCAAAACAGAAATACCTGCATTTGTGAGGCTATCTCCAAAAGA AGAGTTACCTGTGTCAGCTGAAATCAAACACGAAGCTTCAGGGAAGGTCAATGAAATAGATCCACCTTTGTCTGCCTTAG CTGGAGTCAAGAAGAAAGGCAATGTACCGCAGGCTAAGGACATCATCAAGTTGGAAGCTGCAAAACAACAATCTGACAGG AGTGTCGGGGAGAAGAAACTCAGACTTCCTGGAGAGAAAGTTCCATTAGTAACAGAGTTATATGGTTACAATCTAAAGAG GGAAGAATTTGAGATCGAACATGACAACGATGCTGAGCAACTGCTTGCTGACATGGAATTTAAGGATTCTGACACAGATG CTGAGCGTGAGCAGAAACTGCAGGTTCTTCGTATTTACTCGAAAAGGCTTGATGAGAGGAAGCGGAGGAAGGAATTTGTT CTGGAAAGAAACTTGTTGTACCCTGATCAATATGAGATGAGCCTTTCAGCAGAGGAGAGAAAAATATATAAAAGCTGTAA AGTGTTTGCGCGGTTCCAATCCAAAGAAGAGCACAAGGAACTGATTAAGAAAGTCATTGAAGAGCACCAAATTCTCAGAA GAATCGAGGATCTTCAGGAAGCTAGAACTGCTGGTTGCAGGACAACTTCAGACGCAAATAGATTTATAGAAGAGAAGAGA AAGAAGGAAGCTGAAGAAAGTATGCTGCTGCGGCTTAACCACGGTGCACCAGGCAGTATAGCCGGTAAAACACTAAAAAG TCCAAGAGGGTTACCCAGAAATTTGCATCCCTTTGGTTCTGACTCACTGCCAAAGGTCACACCTCCAAGAATATACAGCG GTTTGGACACTTGGGATGTTGATGGTCTCCTTGGAGCTGACTTACTCTCAGAGACCGAAAAGAAGATGTGCAATGAGACC AGAATACTGCCTGTACACTATTTGAAGATGTTGGATATCTTAACAAGAGAAATAAAGAAGGGGCAGATAAAGAAAAAGTC TGATGCTTATAGCTTCTTCAAAGTAGAGCCGAGTAAAGTAGACAGAGTATATGATATGCTGGTTCATAAGGGAATAGGTG ACTCAACATGACTCATAGCTTCATGGTCCTAACATGTACTTCTCGAACATATATTAGCCATGTAAATATAAAGGAAGGTG ATGCTGATTTTGGTTACACATCTTGTTCTC ................................................................................ ............N.......N......N................................N................... ..............N................................................................. ....N............i......................N....................................... ..................i.......................................N.....N............... .....................................................N........................i. ..........................i..........i.......................................... ....N.............i............................................................. ..................................................N............................. .....................N.......................................................... .............................................................N.................. ....................N........N......................i........................N.. ...................................................N............................ ...............................N....N........................................... ................................................................................ ................................................................................ .....................N.......................................................... ................................................................................ ...............N.....N...........................................N......N....... ...........................i.................................................... ..N...............................................N....i........................ .......i..............N..........N.........................N.................... N............................. Pos Score Pred ------------------------ 93 0.183 - 101 0.177 - 108 0.380 - 141 0.120 - 175 0.433 - 245 0.367 - 258 0.562 Yes 281 0.171 - 339 0.724 Yes 379 0.276 - 385 0.105 - 454 0.158 - 479 0.697 Yes 507 0.590 Yes 518 0.507 Yes 565 0.411 - 579 0.544 Yes 691 0.245 - 742 0.472 - 862 0.285 - 901 0.268 - 910 0.169 - 933 0.733 Yes 958 0.100 - 1012 0.151 - 1072 0.106 - 1077 0.294 - 1302 0.320 - 1456 0.357 - 1462 0.179 - 1506 0.467 - 1513 0.276 - 1548 0.626 Yes 1603 0.235 - 1651 0.208 - 1656 0.542 Yes 1688 0.542 Yes 1703 0.144 - 1714 0.069 - 1740 0.049 - 1761 0.056 -

Explain the output -- Processing locus 305: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTGTTTTTAGCAAAACATTAAGACGTGTTAATTGTCAAGAGAAGACCTATGCATATATAATAGTCAAGAAAAGACAA ACTATAATAAAAATCTAATGAAACTGTCTTTTTCAAGTGTGTCGGGGAAAAATGATAAATTCGACCACGATTACGGATAC CACTAATAATTATGGTCACATGCAGATGCAAATGGATTGGTCAAAAACGTCAACTTTGACTTCGCAGCTCTCAAGTAATC TTTTCCCCACTACTAATAGCTCTCTTCTCTCAATCAACACAACACAATTCACCACCATGAACTCTTCCTTTACTCTCTTC ATCTTTACCTTCGTCATCTTCCTCCAATGTCTAAATCCCACCGGAGCTGCCACGTGTCATCCTGATGATGAAGCGGGTCT TCTAGCTTTCAAAGCGGGTATAACCCGAGATCCTTCGGGTATTCTCAGTTCTTGGAAGAAAGGTACCGCTTGTTGTTCTT GGAACGGTGTCACTTGTCTCACCACTGACCGTGTCTCTGCACTCTCTGTCGCTGGACAAGCCGATGTTGCTGGAAGCTTC CTCTCCGGCACTCTCTCGCCGTCGTTGGCTAAACTCAAGCACCTTGATGGGATTTACTTCACCGATCTCAAGAACATCAC TGGTTCTTTTCCTCAATTCCTTTTCCAATTACCAAATCTTAAGTACGTATACATTGAGAATAACCGTCTCTCTGGTACTC TTCCGGCTAACATCGGTGCGCTAAGCCAGCTTGAAGCGTTCAGCCTCGAGGGAAACCGGTTCACCGGTCCGATCCCGAGC TCGATATCTAATTTGACTCTGTTAACTCAACTCAAACTCGGCAATAATCTCCTAACCGGAACTATACCGTTAGGGGTCGC TAATCTCAAGCTCATGTCGTATCTCAACCTTGGAGGTAACCGTCTCACTGGAACCATTCCAGATATTTTCAAATCCATGC CAGAGCTCCGATCTTTAACTCTCTCCCGCAACGGATTCTCCGGGAATCTTCCTCCGTCCATTGCATCACTCGCACCGATT CTTAGGTTCCTCGAGTTAGGCCATAACAAACTCTCCGGGACGATTCCAAACTTTTTATCAAACTTCAAGGCGCTCGACAC ATTGGATCTCTCCAAGAATCGGTTCTCGGGTGTCATACCGAAGAGTTTCGCCAATCTGACCAAGATATTCAATCTTGATC TCTCCCATAATCTTCTAACCGATCCATTCCCTGTCTTGAACGTTAAAGGCATTGAATCTTTGGATCTTTCTTACAACCAG TTTCACTTGAATACGATCCCGAAATGGGTGACTTCGTCGCCGATCATCTTCTCGTTGAAGCTAGCAAAATGCGGGATCAA GATGAGCTTAGACGATTGGAAGCCAGCGCAAACATTCTACTATGATTTCATCGATCTGTCGGAAAACGAGATCACAGGGA GTCCAGCAAGGTTCTTGAACCAAACAGAGTATCTAGTGGAGTTCAAGGCAGCGGGTAACAAACTACGATTCGATATGGGG AAGCTAACGTTTGCAAAGACGCTGACAACTTTAGATATATCAAGGAACTTGGTATTTGGGAAGGTGCCGGCAATGGTGGC TGGACTAAAGACATTGAACGTGAGTCACAACCATCTTTGTGGAAAGCTTCCAGTAACAAAGTTCCCGGCCAGTGCGTTTG TGGGTAATGACTGTCTTTGCGGCTCTCCTCTTTCTCCTTGTAAAGCTTAAGCGGCAAGAAAGCTACAACTGGTAGTTGGA TATTTACCGTGACCAATAAAGTTTAGCTCCAAATAAAATTTGTATTAAAAAGATTACAACAAATAAAGT ...................................................N............................ .................N.................................i............................ ...........N.......N.....N.....i................................................ ........................................................i....................... ..........................N.....................................N..N............ ................................................................................ ...............................................................N................ ..............................................N................................. ................................................................................ ................................................................................ ................................................................................ .............N..............................................................N... ................................................................................ ................................................................................ ................................................................................ ................................................................................ .......................i............................................i........... ..........................................................................N..... ........................................................................N....... ................................................................................ ......N......................................................................... ..................................................................... Pos Score Pred ------------------------ 52 0.193 - 98 0.456 - 132 0.572 Yes 172 0.258 - 180 0.288 - 186 0.360 - 192 0.567 Yes 297 0.776 Yes 347 0.354 - 385 0.394 - 388 0.345 - 544 0.166 - 607 0.380 - 894 0.320 - 957 0.434 - 1304 0.796 Yes 1349 0.870 Yes 1362 0.587 Yes 1402 0.535 Yes 1515 0.267 - 1593 0.461 - 1687 0.256 -

Explain the output -- Processing locus 306: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACCTAACCGGAATTTGGTAATTTGGTTTATAGAGAAGAACTGTTCGGCTCTGCTTTGAAATTTGATTGGTATACGGTTT AGTTCGGGTTTAAAAACTTAATCCGGTCTTAAACCTATTTTGGATCCGGTTTGGGTCGAGCTCAAACACATGTGTCGGAA TTTGGATCCGACAATCCACGACCAGACCACTAACCTGCGATTGGTAAAATTATCTAAGAAAGGCCACGTGTCATCACACA TTCTCCTGACTTCGCCATCATCATTAGCCCCACGCAAAAAGGGAAAACAGAATCCATTCCTTAAATCGATTTCATCCAGA TCTAAACTCGAGCTTTTGTTGATCGAAAAAATGGAAGAGGATTTGATTAAGCGCCTTGAAGCTGCGGTTACGAGGCTTGA GGGGATCTCAAGCAACGGAGGAGGAGTAGTTTCTCTTTCCCGCGGAGGAGATTTCTCCTCCGCCGCCGGAATCGATATCG CGTCGTCCGATCCATCGATTCTGGCTTATGAAGATCTGATTTCTCAATGTGTTGGTAGGGCTCTGACCGCCGCTGAGAAA ATCGGTGGACCTGTTCTAGATGTGACTAAGATAGTCGCCGAAGCTTTTGCTTCCCAAAAGGAGCTGCTTGTTCGCATCAA GCAAACTCAGAAGCCTGACCTCGCTGGATTGGCTGGATTTCTCAAACCGTTGAATGATGTTACAATGAAAGCTAATGCAA TGACTGAAGGAAAAAGGTCGGATTTTTTCAATCACTTGAAGGCTGCATGCGATAGTTTATCTGCATTGGCTTGGATTGCT TTCACTGGGAAAGATTGTGGTATGAGCATGCCAATAGCTCATGTGGAAGAAAGTTGGCAAATGGCTGAGTTTTACAACAA TAAGGTTCTGGTGGAGTATCGTAACAAAGACGCAGATCATGTGGAGTGGGCTAAAGCCTTAAAAGAACTTTATTTACCTG GTTTAAGGGAATATGTCAAAAGTCATTACCCCTTGGGACCTGTATGGAATGCATCAGGGAAACCTGCTAGTGCTCCTGCA AAGGGTCCACCTGGTGCTCCTGCTCCTCCACCAGCACCCCTCTTCAGTGCTGAATCTTCAAAGCCATCATCATCGTCAAA CCAGAAACAAGGGATGTCTGCTGTTTTCCAGCAACTCAGCTCGGGTGCTGTGACCTCAGGTCTTAGAAAAGTGACGGATG ATATGAAGACAAAGAACCGTGCTGATAGATCTGGAGCTGTTAGTGCGGTTGAGAAGGAAACTCGTACCAGTAAACCGGCC TTTTCGAAAACTGGACCACCGAAAATGGAACTTCAAATGGGTCGCAAGTGGGCTGTTGAGAACCAAATTGGGAAGAAGGA CTTGGTTATCAGCGAGTGTGATTCCAAACAGTCTGTGTACATATATGGTTGCAAAGATTCTGTCTTGCAAATACAAGGAA AAGTGAATAACATCACCATTGACAAATGCACGAAAGTGGGTGTTGTTTTCACGGATGTTGTTGCTGCATTTGAGATTGTG AATTGCAACAACGTAGAAGTGCAATGTCAGGGTTCAGCTCCCACAGTTTCTGTGGACAACACAACTGGCTGTCAGTTATA TCTAAACAAAGACTCATTAGAGACAGCTATAACAACAGCCAAATCAAGTGAGATCAATGTAATGGTGCCCGGTGCTACCC CTGATGGAGATTGGGTGGAACATGCGCTGCCTCAACAGTACAATCATGTGTTTACTGAAGGGAAGTTTGAGACAACACCG GTCTCGCATTCAGGTGCCTAAGCAAAGGAGAAACTATTATCACTCTGGATTGTGCTTGAATATCAGGCCTTTTTATACTC ATATACTTTGTTTCTTCAGATTATAATAGAGGAAAGAATC ................................................................................ .....................................................................N.......... ................................................................................ ................................................................................ ..............................i................................................. ................................................................................ ...........................N..................i................................. ...................N............................................................ .....................................................N..N.......N.........N....i ..............................................N................................. .....................i.....i............N...................i................... ......................................N......................................... ............N..............................N....N............................... ................................................................................ .............i...............................................................N.. ..i............................................................................. ........................i...........i........................................... ............................................N................................... .........................N............................N......................... .......................i........................................................ ........................................................N....i.................. ...N.................N.......................N.................................. ................................................................................ ........................................ Pos Score Pred ------------------------ 150 0.233 - 351 0.596 Yes 508 0.121 - 527 0.662 Yes 580 0.142 - 694 0.139 - 697 0.142 - 705 0.454 - 715 0.204 - 720 0.674 Yes 767 0.283 - 822 0.627 Yes 828 0.630 Yes 841 0.173 - 861 0.803 Yes 919 0.345 - 973 0.472 - 1004 0.404 - 1009 0.286 - 1134 0.827 Yes 1198 0.197 - 1203 0.681 Yes 1305 0.604 Yes 1317 0.830 Yes 1405 0.113 - 1466 0.422 - 1495 0.165 - 1544 0.545 Yes 1657 0.335 - 1662 0.724 Yes 1684 0.291 - 1702 0.204 - 1726 0.241 -

Explain the output -- Processing locus 307: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAACCCTTTTATGTGAAGCTTGTGTAGATGTTTTACCTGAATTTCGGAATTACTAGCAACATTTCTAAATACACAAAGC ATGATTGTAAAATGAAATTGATTTTGGTCAGGGCTTTGCATCTCTTGGTTATTGAATGGTGTCCGTGTTTCACTATTATC TTCTTATGTTTTTGATTTTTATGTGCTATTTTCGATAGATTCAGGCGAAGCGACCGCTGATGGCGATAAAGCTTCTACAG AAAACAGCAAAAAACGGAAGCTTAAGACTCCTATGCAAGTTATGGCGCTTGAGAATTTCTACAATGAGCATAAGTATCCG ACTGAAGAAATGAAGGGCAAGTTAGCAGAGGAAGTTGGATTAACTGAGAAGCAAGTTTCAGGTTGGTTCTGCCATAGAAG GTTAAAAGACAAACGTCATGTAAAAGAAGATGGGAATGCCATTGGGAGCCAAGATCGGTCAAGCGTTGTTCTTCAGGATC GTGGAAGTGGGCTTCGACAGGATTCATGTGGTAGTACAAAGCAAACGGATTATTGGAACCCCAAGCCTAGGGAAGTTGAA AGTCAAAGGCTTTATATGGGAAATGCTGATGGTGAAGATAGTACATCTTCTGATCGTAGCTCATCATTACGTAAGAATCT TGTCTCTAGCAAAGATGGAATTCGCGATGTGGAAAGTTCTAGATATGTTGCACATAAAGACGTTATTCAGCATCCTCAGT TCATGAGAAGCTATGGGTATAATAAGCCTTCGGGATACTTGAAAGTGAAGGGAGAGAGTGAGAATTTTGCTATCACTGCC GTAAAGAGACAATTAGGAAGACAATACCAAGAGGATGGTCCACCTCTGGGGGTTGAATTTGATCCTCTTCCTCCCGGTGC ATTTGAGCCTCAGACTAATCCTATAGTTCACGAGCCAATATATGTTGGGAATCAGAGACGGCCTCACCTTCCACATTTAC TTGGAACGAGGAAGAGCTTTAATCCTGGTCCTAGCTATGAATTAGCTCGTAAATCAAAGTTGCATTCGCCAGATCCAGAT TCAGAGGATGATGAGCATGATGATGATGACAACATAATGGTTGGAATGGAGCCTGGTCTAAGAGATAAGAAAAGTTTTGG GGAACCAAGACTGAAATCCCCTTCTACCAGTTTCTATAATTCTGTACCTCGCCACAAATCGTTTAAAGAGACATTCAAAG GTTCACCACGGGAAATCCCTGTAACTAACAGCAAGAAGGGTTGGATAAGTTCCAAAAGTTGGGCTGAAGGGTCGAGAAAC CATCTGGTTGCCAACGTCCAAAATCTTTCTGGAAGCAATATTGAAACCAATCAGTCTCATGATTATGATAATAATATATC TAATGGCGGACGCAAGACGGGATATCTCACTAAATCTTCGAAGCTGTTACCTCCGAGTAGAAGCAGATCCCCTGAGTCAA TGGATAGAGGTCCATCTTCTGGGATGGCAGGAATATATCACGGGGAGAGGAATCAAATGAAAATGCAGAGAGAGAAATTG CATTCGACTGATGAACCACCGGTTGCCAAACGAGTGAAACATGGGTATATTCAGCAAGTATATGCACCAAAATCATCATC ATACAGCGAGATTCTTGAACGAAAAAGCCAGATAAATCGGTCTGGTGTGGAACTGCCTTCTAGTTTAAGCGGGGACGATG AGACTGACGAATCTAGCTCGTCAATGGATTAAGTAAAGAAAGGTACAAAGGAAGATTAGGAAATTCCGGTTTGTATAGAT ATATGATGAAACCGGTTTAGGATGTGAAAATGTTGCATCGTGTAATTGGTA ...........N................N................................................... N..........N...........................................N........................ .....N..............N......................................N.................... ................................i........N.....................N................ .........i...................................................................... .................N...........i.....N............................................ .........................N...................................................... ...............i......N.....N................................................... ..............N...........N.................N................................... ..i.........N................................................................... ..................................N............................................. .........................................i...................................... ....................................N........................................... .......N..N.....N..N..N..N..........i........N.................................. ................................................................................ ................................................................................ ..........................................................N.....N............... ..N............................................................................i .......................i................................N.....i................. ..........N.............................N....................N.................. .............................................................................N.. .......................N........................................................ ..N..N...............N.......N..................... Pos Score Pred ------------------------ 12 0.171 - 29 0.341 - 81 0.084 - 92 0.184 - 136 0.188 - 166 0.176 - 181 0.271 - 220 0.241 - 273 0.578 Yes 282 0.487 - 304 0.334 - 330 0.676 Yes 418 0.346 - 430 0.581 Yes 436 0.398 - 506 0.246 - 576 0.621 Yes 583 0.211 - 589 0.267 - 655 0.356 - 667 0.379 - 685 0.193 - 723 0.701 Yes 733 0.474 - 835 0.204 - 922 0.627 Yes 997 0.217 - 1048 0.280 - 1051 0.113 - 1057 0.118 - 1060 0.182 - 1063 0.251 - 1066 0.173 - 1077 0.644 Yes 1086 0.422 - 1339 0.243 - 1345 0.155 - 1363 0.436 - 1440 0.641 Yes 1464 0.822 Yes 1497 0.368 - 1503 0.521 Yes 1531 0.292 - 1561 0.238 - 1582 0.168 - 1678 0.171 - 1704 0.218 - 1763 0.075 - 1766 0.051 - 1782 0.036 - 1790 0.058 -

Explain the output -- Processing locus 308: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACATTTCTCGACACTGAAGAAACTAAAAAATCGAATAAAGCAGAATTACTCTACCGGGTTAAACCCGGTCTGGATTATC ATTCACCCGACCCGGTTCTAAACCCGTTTTAAAAGTAACGCGCTCGGGAATATCAGTACATGGGCCCAAATTACTATGCT GATACATTCAGTTAATGATAACGTTACCGTCACAGTAAAGCTAAACAACTTCGATTCGATTAAAAAAAAACTCGCCGCTT ATAAACGGCGTCTGCCCAAAAAAGTAGGAAACTCAGAAGTAAAAAAAAACCAAAATCGCACCTCCATGACCACCGTCTCC TCCGCTTTCGCCACCGTAGCGGAAGGCGCGAAGGTTGTCACACCGAGATGGAAGAAGAATGAAAGAAATGGAAAGTTACG GGTGATGTGTAGGTATGGCGGTAGTATAGTTTCACCTCCGCAGACGAAATCGCCGCGTTATGTAGGCGGAGATACTCGAA TCGTCGCCATTCCTTCTTCCGCGGAAACGAGCTTCGCTTCTCTTGTTAGCCACCTTACGGTTACGCTCAAAATCTCTTAT CCTTTCAAAGTTAAATATCAGCTTCCTGATCAGGAACTAGATTCGCTTATCTCCGTCGAGGCGGATGAGGATGTTCAGAT CATGATGGAAGAACACGGTTATCTCTCCTCCGAGTCCTCGATTCCCCAATCGCGTATCCGATTGTTTCTTTTCCCATTGA AATCACAACAATCGAATGAAGCTGGTGCGAGTCAGGGAGATTCGGATCAGTGTAAAGTGGAGACCGATATCGATTGGCTT GGAATCGAAGAGTCTAATAAGCCTATTCGTGAAGAATTGACTCAGCCGGTACTTCAGCACCCTAAGACGGAGATGTGGTT CGTAGACGCTTTGAAGAGCGTGGAGATGATGCAGACTAGACGAACTAATAGCGGAACTAGCGGAAGTGGAGATGGTAACG GCGGGATTTGTGGACAGGAGTCGATGATGCTAGAGACTAATTCATCCTTTGGCTCGACATCATCCTCGGTTTCGTCGAGC AATTTACCTCCGATAAAAAGCTCCGGCGAGGACAACATAGCGAACTCGCAAGTCAAATTTGCGCCAATAGAATCAGTGAC AAGCAACGACAATTCTGCAGTCACCCCAATACCTTCCCACGAGCTACCATCACACTCACATGCTTTTGAGAACAAACCGT CTTCAAACTTGTATGTGGCTGAGCTAAACAGACCAGTTCCAGTCCCAATTTCCGGGTATCCACCATTTATGAACCAAGCT CAACAACAACATATCCAAGTCATTTACACTGGACAACCTTACATTACCGGAAACTCACCTATGACATTGCCTGCCACAGC ATACCATCACACAAACCACGTCTATTATCAGCGTCCGCCACAACCATACCCAATCTATTACATTCCAGTCGAGCAGTACA GTTCGAGGCACGTTCAAGCACTGCCTGTGAAACCGAGCACCGTTTTAAACTATCACCAAGTTGATTCCCCGGTGGTCCGC ACCAGCAGCCCTCTAGCGCCAGAGTTTTCCTCACAGGTTTATCCTCTATCCAAACCCGTTGATTCATCGGTACAGACATC AAGTGAAGCTACTCTTAATACTACTTCAAGAGATGCTTTCATCTATAATACCGATGTCGATGACGACAACGATATAGCTC ATGCTCAAATTTACAAATCTCAGCCTCCAGCTCCCACATTACCGTCTCAGTATTAAACCATTATGGTAACCGAAGCTTTC AGTCAGGTACAGACACACTATGCTTGAGAGATCTAAGAATCCCATCCATTCTCATGTTGCTGCACTTTCAGGTAT ................................................................................ ...........................................................i...............i.... ..............N................................................................. .................................................................i.............. ...............................................i..........N........i............ ....i.........i............................................N.................... ................................................................................ ................................................................N.....N......... ...............N................................................................ ........................................................................i....... .........................N..N..........................................N........ .......................N..i..................................................... ................................................................................ ...........................................................N.................... ............N.......................................................N........... ............................................................i................... ................................................................................ ................................................................................ ................................................................................ ................................N....................i.....N.................... N.............................................................N................. ...................N.................................N..................... Pos Score Pred ------------------------ 140 0.671 Yes 156 0.644 Yes 175 0.307 - 306 0.552 Yes 368 0.567 Yes 379 0.454 - 388 0.810 Yes 405 0.670 Yes 415 0.543 Yes 460 0.122 - 625 0.260 - 631 0.318 - 642 0.573 Yes 645 0.358 - 736 0.467 - 873 0.765 Yes 906 0.249 - 909 0.471 - 952 0.479 - 984 0.326 - 987 0.599 Yes 1180 0.228 - 1213 0.134 - 1269 0.218 - 1341 0.545 Yes 1633 0.230 - 1654 0.542 Yes 1660 0.143 - 1681 0.130 - 1743 0.175 - 1780 0.088 - 1814 0.061 -

Explain the output -- Processing locus 309: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTCCAGCAAATCCAGCGATGCATCAACCTGTATTGTCGCCAAATTTCAATCTCTATGTCGTTTCCTATTTAGTAACCG CACTGCTTGACGTTTATTTAAATAGAAAACGACATGCAGTCTGTTTATAAAAATAAAGCTGTAACCTTTAAAAAGTTGCT ATCTTTGCACCCGAGAGAGAGCTCAGTGAAAAAGATGGGAAGCGGTGTAGGTGTAGCTCTCTTTGCCCTCTCCCTTCTTC TCGTCTCCCATGAGGTGGAATCTGCAATCGGCGTGAACTGGGGAACGTTGTCATTTCACAAGATGAGACCTTCCACAGTG GTGGATCTCCTCAAAGCAAACAAGATCACCAAGGTGAAGCTTTTCGATGCGAATCCAGATGCGCTTCGAGCGTTAATGGG TACTGGAATCCAAGTAATGATCGGCATTCCTAACGAGATGCTCTCAACTTTCAATTCTGATTTGTTTGTCCAGCAAAATC TCTCTCGCTTCATAGGCAAAAACGGTGCTGATATTAGGTATGTTGCAGTAGGGAATGAGCCGTTTCTGACTGGTTACGGT GGTCAGTTTCAGAACTATGTAGTCCCAACTATGGTTAACCTGCAACAATCTTTGGTTAGAGCAAATCTAGCTAGCTATGT GAAGTTGGTGGTTCCTTGCAACGCAGATGCGTATCAGTCTAATGTTCCCTCTCAGGGGATGTTCCGGCCTGAGCTTACAC AGATTATGACTCAATTGGTTTCGTTTCTCAACTCCAATGGCTCTCCTTTTGTGGTTAATATCTATCCTTTCCTTAGTCTC TATGGGAACTCGGATTTTCCACAAGACTATGCCTTTTTTGAGGGGAGTAGTCATCCGGTTCCGGATGGGCCTAACACTTA CTATAATGCATTTGATGGGAACTTTGACACTCTTGTTGCTGCTCTCACCAAGCTTGGATATGGTCAAATGCCTATAGTTA TTGGTGAGATTGGTTGGCCTACAGATGGAGCAGTTGGTGCTAATCTCACAGCAGCCCGGGTTTTTAATCAAGGGCTCATT AGCCATGTGTTGAGCAACAAAGGAACACCGCTCAGGCCTGGATCTCCTCCTGCGGATGTATATCTTTTCGGTTTGCTTGA CGAAGGAGCGAAAAGCACACTGCCTGGTAACTTTGAGAGGCATTGGGGAATCTTCTCTTTCGATGGACAGGCCAAGTATC GTCTCAACTTAGGACTAGGCAACAGAGGACTCAAGAATGCAAAAAATGTGCAGTACCTTCCTTCAAGGTGGTGTGTGGCT CACCCATCAAGAGACATGACCCAAGTTGGTGACCACCTCAGGCTTGCTTGCAGTGAAGCAGATTGCACAACGCTCAACGA CGGGGGCTCGTGCAGCCAGCTTGGTGAAAAAGATAATATCTCATACGCTTTCAACAGTTACTATCAGATGCAGATGCAAC ATGAAAAGAGCTGTGACTTTGATGGACTTGGCATGGTCACCTTCCTAGATCCTTCAGTTGGTGACTGTCGATTTCTTGTG GGTGTTACTGATATAGGCTTATCGTCAAGTACTGAGCCAATGGCAAGGTGGTCTATCTACCACATTTGCATTGGACTATT GATCTGGACTCTCACAATGTAGATTAGGGAAATTTTTCAGTGTTACATTATTTAGCGATATAGTAGATATAGGACGGAGA AATTGCATAAATATAGGGTCAGATGTATTTTATTGTTAAGC ...................i....................................N....................... .................................N.............................................. ..................................i............................................. .........N....................................................i................. ..............................................N...........N................i.... ................N....................N.......................................... .......................................N..............N......................... ................N.............i.............................................N... ..........................N..............N................N..................... .....N..............................i........................................... .....N........N............................................N.......i............ ........................N....................................................... ....N..................................................N........................ ..............................................................i................. ....................................i........i.................................. ...............i................................................................ ...................................................................i.....N...... N....................N..........i............................................... .......................................N........................................ ................N............................................................... ......................N.................. Pos Score Pred ------------------------ 20 0.591 Yes 57 0.457 - 114 0.346 - 195 0.677 Yes 250 0.168 - 303 0.831 Yes 367 0.147 - 379 0.087 - 396 0.604 Yes 417 0.359 - 438 0.205 - 520 0.163 - 535 0.093 - 577 0.302 - 591 0.653 Yes 637 0.202 - 667 0.089 - 682 0.162 - 699 0.181 - 726 0.312 - 757 0.799 Yes 802 0.426 - 829 0.226 - 865 0.168 - 886 0.280 - 895 0.192 - 940 0.200 - 948 0.909 Yes 985 0.414 - 1045 0.405 - 1096 0.159 - 1183 0.515 Yes 1237 0.618 Yes 1246 0.541 Yes 1296 0.738 Yes 1428 0.502 Yes 1434 0.425 - 1441 0.235 - 1462 0.290 - 1473 0.556 Yes 1560 0.248 - 1617 0.353 - 1703 0.049 -

Explain the output -- Processing locus 310: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTTCAGTGCATTGCCAAACAGTAAACACCAATAAAAGCTGCAAAACACCCAAAAAAAAGAAAAAAGCCCGCCTATTCC CGGAGAAAAGAGAAAATCTGAAAATTGAAATTGACAACTTTTTCTCGGCCTTGGTGTTGTTCCCAAATCCAGAGACACAC CATGAGCACTCCCATGGAGATTGCCGAGCAAAAACCTATGGTGGAGGACCCGATTCCTCTTCCAAACGCTTCCATGGAGG TCTCTTACCAAAACCCCATTGAAGCAGCGACTATTCCAGTGCAAATAGCCGTCGCTGAGCCAGTCGCTACACCCAATCCT CCTCCTTGCCTCCACGAGAACAAATTCCTCGTATCTGTGGAGGTCTGCTTAAAGCCTTCAAGCACGGCTCGTCTAGAGGA TGTCCAGAGAGCTGTAGAACGGATGCTGGAAAATAGGAGCATGAGCTATGCAGATGGACTGGTTTTGATTCCTGCAGACG ACTTGTTCCTAGTTGATAATGTGCAAAGGATATGCATTTGTGATACAGAGGAATGGGTGAAAAATAATGATGTTCTCTTG TTCTGGCAAGTGAAGCCAGTTGTGCACACCTTTCAGCTCATTGAAGAAGGACCATGTGAGGACTTGTGTGCAGATGGACA ACCTGCTAGCTTTAACGAATGGATTCTACCCGCAAAGGAGTTTGATGGCTTATGGGAAAGCCTAATATATGAATCTGGAC TTAAGCAAAGGTTACTACGTTATGCTGCGAGTGCATTGCTGTTCACCCAGAAGGGTGTTAACCCAAATCTCGTTTCATGG AACAGGATTATTCTTTTGCACGGACCACCAGGGACTGGCAAAACATCCCTTTGCAAAGCGTTGGCTCAAAAGCTTTCAAT CCGGTGCAACTCCAGATATCCACATTGCCAATTGATTGAAGTCAATGCTCACTCTCTATTTAGTAAATGGTTCTCTGAAA GTGGCAAGCTGGTTGCCAAGCTTTTCCAAAAAATCCAAGAGATGGTGGAGGAAGATGGAAATCTGGTATTTGTTTTGATC GATGAAGTAGAAAGTCTTGCTGCTGCTCGAAAAGCTGCATTGTCTGGCTCAGAACCGTCAGATTCTATCCGGGTTGTGAA TGCACTACTCACCCAAATGGACAAATTGAAATCAGCACCGAATGTGATAATCCTTACAACATCAAACATAACTACTGCTA TTGATGTTGCTTTCGTGGATCGAGCTGACATAAAAGCTTATGTTGGCCCTCCAACTCTTCATGTTCGTTATGAAATATTA AGGTCTTGTGTTGAGGAATTAATAAGTAAAGGGATCATATCAAGTTTCCAGGGCTGTGATGGACTCTCTATTCCAAGCTT TTCAAGTTTGAAAGAAAAGTTAAGTGAAAGTGAGGTTCATGATACAAATACAGTTCCTTGGTTCTGCAAACAGTTGATAG AAGCTGCAAAAGGGTGCGAGGGTTTAAGTGGGAGATCATTGAGGAAGCTTCCGTTTTTAGCGCACGCAGCACTTGCAGAT CCATATAGCCATGATCCAAGTAATTTCTTGTGTACAATGATAGAAACAGCTAAGAGAGAGAAGTCTGAACAACCTGAATG ACCATTTTTGTGAGGTCATAAACAAATAACAATGAAGTTGTGTTGTTCAGAAAAATGTTATGCTAATTGTTGATTAAACA TCACTTCATCATCTACAGCT ................................................................................ ................................................................................ ................................................................................ ...............................................................................N ......................i.................i......N.....N.......................... ..................N............N....................i.............N..N.......... .....................................................N...................N...... ..................N.........................i......N................N........... .....................N......................................................i... ................................................................................ ............................................N.....................i............. .........................................i............N......................... ................i........................N...................................... ...N...................................N....................N........N.......... ..........................................................N..................... ......................................N......................................... ................................................................................ ..........N.........................N........................................N.. ...............................N......................N....N.................... .................... Pos Score Pred ------------------------ 162 0.747 Yes 174 0.769 Yes 198 0.765 Yes 234 0.516 Yes 400 0.221 - 423 0.670 Yes 441 0.705 Yes 448 0.197 - 454 0.305 - 499 0.199 - 512 0.464 - 533 0.819 Yes 547 0.256 - 550 0.210 - 614 0.395 - 634 0.225 - 659 0.427 - 685 0.573 Yes 692 0.397 - 709 0.406 - 742 0.447 - 797 0.642 Yes 925 0.333 - 947 0.508 Yes 1002 0.802 Yes 1015 0.366 - 1042 0.145 - 1120 0.071 - 1137 0.646 Yes 1162 0.097 - 1204 0.169 - 1240 0.446 - 1261 0.085 - 1270 0.123 - 1339 0.181 - 1399 0.135 - 1531 0.271 - 1557 0.276 - 1598 0.113 - 1632 0.109 - 1655 0.045 - 1660 0.044 -

Explain the output -- Processing locus 311: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCTTCTGATTAAAATTAGGTTTCAAGTGGTTATCCTCTATTGTTCATAGTTTGAGCTAGGTTTAGAGTTTGTGATATCT TATCCATGTTTATGAATTTGATTAGCTGAATCAATCTCTTTCCTTTTATATATGATCATGAAACAGGAGATGATGGCTCG TTACCACAGGAGATCGATGATCTTGTGCTTAGTTCTGCTCTCATTAAGCATATCGCCGAGCCAAGGCTTCCTAGGGACAG AGGAAAAGATCAAATCCGCTGTCTTTTACTCACCAAAACTTGTGATGAATCCAGGATCAGTTGCAAATCCTTACTTATTC GACATCGATTTCCCAAGAGGTCATATCGGCCTCAAAGCTTTCGATGCTGAAGTGGTTGACGAAGCGGGCAAACCCGTTCC TCTACACGAGACCTATCTCCACCATTGGATTGTCGAGCCATACTATGTACGTAAAGGTTCCAAGCTTCCACAGAGAGAAA TGTTCAGGAATCATGGATTTTCCAGGGAAGATCCTGAAAGCAATCTTGATTCCAAGTCAGATATTATTCTTGTGAAAAAT GGAGGGTTGTGCAGGAGTTTAACACTCAGACATTTCTTTGGTTTAGGATCAGAAACCCGCGAAACCTCGACGTATGTTCC TGATCCCTACGCTATTGAAATCGGTAATCCTGAGGAAACACCTGATGGTTATGAATTCAAATGGCTTCTTAACATTCACG CGATAGACACCAGAGGTGTGGAAGATAAGAAAGGATGCATAGAATGTTTGTGTGATCTGTATAATGTTACCATTGATGAG TATGGTCGAGCTATAAGGCCTGGATACAAAGGAGGTTTATACTGCTGCTATGATAAGACTCAATGTCGGGTGAAAAGCGG ATTTGACAATGGGGAGAAAACAAGAACTCTGTATTTGAAGTATACCGTGAGATGGGTTGATTGGGACAGCTCGGTTTTGC CCGCTAAGGTTTATATTTTCGATGTTACAGATTCTTGGGAAAGATCAAAAGGAGATAGCCAAGAACATATTTGCCATGTG GAATACGAGGTGAAACCGTGCAAAACCAATGGCGATGGATGCGTTGACGTTAAGAAAAAGAGCTTAGTGATGCCATTTGA TGGGTATATTGTCTACGGGGTAGCTCACCAGCACGCTGGTGGTATCGGTGGTGCTCTATACCGAGAGAACGGTGAAGGGA TATGCGCTTCAATGCCGAAGTATGGCAATGGAGATGAACCTGGAAACGAAGCTGGTTACATTGTTGGAATGTCGTCTTGT TATCCAGCTGATCCAGTGAAAGTGAGCTATGGAGAGACATTGACTTTGGAATCTAATTACAGTAATGCCGTTGGTCATAC CGGAGTTATGGGACTCTTTTACATCCTCGTTGCTCAGCAGCTGCCGGAACCAGATAGCTCCTTGCCTAATAAACAACATT TTGAGGCACCTGCGAGAAGCTTGAGCTTTTTAGCCATTTTTGCAGTGACGGTAGTTGTGGCTGTTGTGGTTTTAATAGCC GCCGTGATATACCGGAGACAGAAACGGGAAGATGGTTACCAATCACTTAGTACTTAAAAAAGAGCAAAAACCATGTCGAC TCTATATATGTCTTCAAAGTGAATTCTGTTTGTATACATAGAATAAAAGATTATTTTGTTGTCATCTTCCTCTGAT ................................................................................ .....N.....N.......................................N.....N...........N..i....... ................N............................................................... ............................................i................................... ...........................................N.................................... ............................................N..................................i ............i.................................................................N. .........................................................................N...... ............................................N.....N.........i................... ..................................N........N...................i...........N.... ........N..........................................N............................ .....................i.....................................................N.... ............................N.....N...N..............................i.........N ................................................................................ ............................N...................................N............... .......i........................................................................ ................................................................................ ...............................N........................................N....... .......N.................................................................... Pos Score Pred ------------------------ 86 0.272 - 92 0.343 - 132 0.220 - 138 0.447 - 150 0.236 - 153 0.517 Yes 177 0.235 - 285 0.797 Yes 364 0.390 - 445 0.331 - 480 0.673 Yes 493 0.688 Yes 559 0.483 - 634 0.281 - 685 0.164 - 691 0.091 - 701 0.517 Yes 755 0.211 - 764 0.164 - 784 0.541 Yes 796 0.200 - 802 0.535 Yes 850 0.225 - 863 0.657 Yes 889 0.473 - 932 0.212 - 982 0.505 Yes 1036 0.322 - 1069 0.472 - 1075 0.301 - 1079 0.190 - 1110 0.881 Yes 1120 0.258 - 1202 0.291 - 1212 0.674 Yes 1222 0.355 - 1228 0.256 - 1234 0.305 - 1269 0.535 Yes 1309 0.347 - 1345 0.370 - 1368 0.565 Yes 1552 0.388 - 1593 0.309 - 1608 0.085 -

Explain the output -- Processing locus 312: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGGCTTCATTAACTCATGTTTCGGTTCTTTATATATTTTAATGAAATATTTAATGACATAGTGAGCGATGTTCTCTGTCT CTTGCAGCATTTTACATTTAGTACTATTCTCCAAAGATTTGGTTTCTTAGATTTTCTGAAATCTTTTGTCTAAGTCTTAA ATACTTGTCTTCTTTGGTAATGCAGTTTCATAGAAACCCAATGGACAACAACAACAACAACAACACTTTTAGTTCTCTGG ATAATGTCATGACTAACCAAAATCCTCTTCTCATGGATTTTATACCTTCAAGAGAAGATTCAACTTCATTCTCAACAATG CTTCCATGGAATACCATCAGATCAGATCCTCTACAAATGGGTGGCTTTGATATTTTCAATTCTATGCTGACTAACAAATA CTTATCATCTTCTCCACGGTCTATCGATGTTCAAGATAACCGCAATGTTGAGTTCATGGCTCCTCCTCCTCATCCTCCTC CACTTCATCCTTTGGATCATTTAAGACACTATGATGATTCCTCAAACAACATGTGGGGTTTTGAAGCAAATAGTGAGTTT CAGGCATTTTCAGGTGTAGTTGGTCCAAGTGAACCAATGATGTCTACATTCGGTGAAGAAGATTTCCCGTTTCTAATTTC GAATAAAAGAAACAATGAGCTTTCATTGAGTCTTGCATCAGATGTTTCTGATGAATGCTCGGAGATAAGTCTTTGTGCAG CTACAAGATTAGCCTCAGAGCAAGCTTCTTGCAGCAGCAAAGACATTTCTAATAACGTTGTTACTCAAGGTTTCTCTCAA CTTATATTTGGCTCAAAATACCTTCACTCTGTTCAAGAAATACTATCTCATTTCGCCGCATACTCGCTCGATTATTCATC TCGAGGAACCGAGTCAGGAGCTGCTAGTTCAGCCTTTACTTCACGTTTTGAGAATATAACTGAGTTTCTTGATGGTGATT CTAATAACTCGGAGGCGGGTTTCGGATCTACATTTCAAAGGAGAGCATTAGAAGCAAAGAAAACCCATCTCTTGGATCTT CTTCAAATGGTGGATGATCGATATAGTCATTGCGTAGATGAGATTCATACGGTTATATCAGCGTTCCATGCTGCAACCGA GTTAGATCCACAGTTACACACCCGGTTTGCCCTCCAAACCGTTTCCTTCTTATACAAGAACCTGAGAGAGAGAATCTGCA AGAAGATAATCTCTATGGGATCTGTATTGGAGAGAGGCAAAGACAAGACTCAAGAAACCTCTATGTTCCACCAGCATTGC CTTCTTCAGCAGCTGAAACGAAAGAACCATCAGATTTGGAGACCTCAACGAGGTTTGCCTGAGAAATCTGTTTCGGTTCT ACGGAATTGGATGTTCCAAAACTTCCTTCACCCTTACCCGAAAGATTCGGAGAAACATCTTCTAGCTATACGAAGTGGCT TGACAAGAAGTCAGGTATCAAACTGGTTTATAAATGCGCGGGTTAGGCTATGGAAGCCGATGATAGAAGAGATGTATGCG GAAATGAACAAGAGGAAGCTCAATAACAGTCACATTCAACCCAACGGACCAACTCTTCGAATGCCAAAATCTGTTATGAT GAGCCAAGCAATGCATAAATAAGACAACAATTGTGTTTACCAACTTTGTGATAATTAGGCAATTGCTACTCTATGATTGC CCAAAACCTAAACCATGTACGACTATCATTACGTATGTTAT ...............N........................N...........N..............N............ ................................................................................ ...................i....................i....................................... ...N....N.......................N............................................N.. .....N..............................i..........................i................ ..........................N.................i..........i........................ ..............................N..N................i............................. ....................................N..i........................................ ..............i..........................N........N...N......................... ................................................................................ ................................................................................ .......................................................................N........ ................................................................................ ......i......N.......................N.............................N............ ................................................................................ ..............N...............................................N................. ................................................................................ ..........N..................................................................... .................................N...............i.........N...........N...N.... ...N........................................................N..............N..i. ..........N.............................................................N....... ..............N...................N...... Pos Score Pred ------------------------ 16 0.191 - 41 0.150 - 53 0.312 - 68 0.375 - 180 0.522 Yes 201 0.564 Yes 244 0.210 - 249 0.432 - 273 0.378 - 318 0.498 - 326 0.315 - 357 0.803 Yes 384 0.585 Yes 427 0.391 - 445 0.569 Yes 456 0.599 Yes 511 0.377 - 514 0.281 - 531 0.809 Yes 597 0.499 - 600 0.709 Yes 655 0.716 Yes 682 0.194 - 691 0.333 - 695 0.264 - 952 0.150 - 1047 0.592 Yes 1054 0.393 - 1078 0.167 - 1108 0.312 - 1215 0.333 - 1263 0.273 - 1371 0.149 - 1474 0.206 - 1490 0.660 Yes 1500 0.237 - 1512 0.455 - 1516 0.308 - 1524 0.391 - 1581 0.417 - 1596 0.439 - 1599 0.552 Yes 1611 0.381 - 1673 0.060 - 1695 0.099 - 1715 0.041 -

Explain the output -- Processing locus 313: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCTGCTTCCTCCTCCTCCTTACTTCTTGTTTCCATCTTGGTTTCAAATTTCTTCAAAGGTGAATTATTTTCTAAAGTTT CCATTTTTGTTAACAATATAAAAAAAAAAATTGAAGTCAAAGAAGACTGTTCCATTTTTTGGGTTTCATATAAAAAAAAA ATGGGTGATGACATTTTCTGTAATCTTTCATTGTATGGATTTGTTTAGATTCCGATCATCTTCACGTCGGAGTACACAAT CTGAAACCGATCATCATCATCATCAATTGGGGTTTTTAATCCCTAGAATCAGTAGAAAATATCGATCATTGAGCTTCGAT TCTGGTCTCCGTTTGATTGTTTCGGACAATCAGAGCTTTGAGTCTCCGAAAAGGTTGGTTATGTCATCTTCTGTTCCAAA CTCGGACCGGAAGATTGTTACTGGTCCCGCCGGTTATATTCTTGAAGACGTTCCTCATTTCTCCGACGACTTTCCTGATC ATCCTACATATCCAAATCCATTACAAGACAATGCAGCTTACTCTGTTGTTAAGCAGTATTTTGTCGATGAGGATGACACG GTTCCTCAAAAAATAGTAGTTCATCCTGATAGTCCTAGGGGAACGCATTTCCGTCGTGCAGGACCACGTCAAAGGGTTTA CTTTGAGTCGGATGATGTTCTTGCTTGCATTGTTACATGTGGCGGTCTATGTCCCGGGCTTAATACTGTGATCAGAGAGA TAGTTTGTGGATTATCTTATATGTATGGTGTCAAGAGAATCCTTGGCATTGATGGAGGTTACAGAGGATTTTACGCGAGA AACACAATCCATTTGGACTTAAAAACAGTGAATGATATCCATAGAAGCGGAGGAACTATCCTCGGGACTTCAAGAGGCGG TCACAACACCACCAAGATAGTAGATAGTATTCAAGATCGCGGGATTAACCAGGTTTATATAATCGGTGGAGATGGATCAC AGAAAGGAGCAGCTGCTATATTCGAGGAGATTAGGAAACGCAAGCTTAAAGTTGCGGTTGCCGGAATCCCGAAAACAATT GACAATGACATTCCTATTATCGATAGATCTTTCGGGTTTGACACAGCTGTAGAAGAGGCTCAACGTGCTATCAATGCAGC ACATGTCGAAGCTACAAGCTTTGAGAATGGTATTGGTCTTGTGAAGTTAATGGGACGGTACAGCGGATTCATTGCAATGC ATGCCACTCTAGCCAGCCGAGATGTGGACTGTTGCTTGATCCCGGAATCTCCATTTTTTCTTGAAGGCTCGGGCGGGCTT TTTGAATTTATCGATAAACGGTTAAAGGAGAGTGGTCATATGGTGATTGTAATTGCAGAAGGTGCGGGACAAGATCTGTT GTCTGAAAGCATGAAAGAGTCCACAACTCTCAAAGATGCCTCTGGAAACAAACTTCTTCAAGACATTGGCCTATGGATCT CCCAAAGAATCAAGGATCATTTTGCCAAGAAGATGACCCTTACCCTCAAATATATAGATCCAACCTACATGATACGAGCT GTTCCGAGCAATGCATCAGACAATGTATGCTGCACGCTTTTAGCTCAAAGCGCGGTTCATGGAGTGATGGCTGGTTACAA TGGTTTCACTGTTGGCCTTGTCAATGGAAGACATACTTACATTCCCTTCAATAGGATCACGGAGAAACAGAACAAGGTGG TGATCACTGACAGAATGTGGGCGAGGCTTTTGTCTTCGACGAACCAACCGAGTTTCATGAAGCAAGCTGACAAGATCCAC TCAAACCAGTTGGTTGGTGAACCAGGGACCATGAAATGGTGATCAACACTGTCTACAAAAACATTCGTTTGTGTTTTGAC TTGAGGACATCTTTGTTTGGGAAGTAAGGTTTCTTAATTTAATAGAAAGCTTTTCAAAAAT ................................................................................ ................................................................................ i......N..........................N............................................. ................................................................................ ............................................................N................... ................................................................................ ..............................N...................................N.....N....... ................................................................................ ...........N..N.....................N...........N............................... ....................i...N..........................N............................ ...............................N................................................ .......................................................................N........ ................................................................................ ....N....................................................................N...... ..N.......................N......................N..........................N... N....................i.......................................................... .......................................i........................................ ..........i........................N....................................i....... ................................i...................................i........... ..........i...........N...N...............................N.......i............N .......................N........................................................ ..............i.........................................N....................... ..............................N....N............................................ ............................................................. Pos Score Pred ------------------------ 161 0.632 Yes 168 0.289 - 195 0.207 - 381 0.428 - 511 0.451 - 547 0.167 - 553 0.328 - 652 0.155 - 655 0.148 - 677 0.353 - 689 0.189 - 741 0.750 Yes 745 0.280 - 772 0.395 - 832 0.185 - 952 0.308 - 1045 0.173 - 1114 0.161 - 1123 0.372 - 1147 0.339 - 1170 0.425 - 1197 0.460 - 1201 0.350 - 1222 0.733 Yes 1320 0.667 Yes 1371 0.585 Yes 1396 0.384 - 1433 0.533 Yes 1473 0.511 Yes 1509 0.530 Yes 1531 0.536 Yes 1543 0.210 - 1547 0.083 - 1579 0.247 - 1587 0.850 Yes 1600 0.251 - 1624 0.319 - 1695 0.728 Yes 1737 0.128 - 1791 0.277 - 1796 0.324 -

Explain the output -- Processing locus 314: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGAAGGATTAAGATCTGGTCGTGTTCAATATATCTCCGCTGATCTTCGAGATAAATCTCAAGTCGTTAAAGGTTCTTAAT CACAAAGTCATTGATTCTTAGTGGACTTTGAGATCTATGCATCTTTGTAATTACTTGTTCCCATTAGCTTTTCAGGGAGC GGAGGTGGTGTTTCATATGGCAGCTCCAGATTCTTCTATTAACAATCATCAGCTTCAGTATTCGGTGAATGTTCAAGGGA CACAAAATGTGATTGATGCTTGTGTTGATGTTGGAGTAAAGAGGCTTATTTACACTAGCTCTCCTAGTGTTGTGTTTGAT GGTGTTCATGGTATTTTGAACGGTACTGAATCAATGGCTTACCCAATTAAGCACAATGACTCATATTCAGCAACTAAAGC TGAAGGAGAAGAGTTGATCATGAAGGCTAATGGTAGAAATGGACTACTCACTTGTTGCATACGTCCAAGCAGTATTTTTG GTCCCGGTGATAGATTATTGGTTCCGTCGCTTGTTGCTGCTGCTAGGGCTGGGAAATCTAAGTTTATTATTGGTGATGGG AATAATCTCTATGATTTCACTTACGTTGAGAATGTTGCTCATGCCCATGTTTGTGCTGAGCGTGCTCTAGCTTCAGGAGG AGATGTTTCTACAAAAGCTGCAGGACAGGCATATTTCATTACCAACATGGAGCCAATTAAATTTTGGGAATTTATGTCAC AGCTCCTTGATGGACTTGGTTACGAGAGGCCAAGCATAAAGATCCCTGCGTTTATCATGATGCCGATTGCGCATCTGGTT GAACTGACATATAAAGTGTTGGGACCATATGGGATGACAGTACCACAACTAACACCTTCAAGAGTTAGGCTTCTCTCATG CAGCAGAACTTTCGATTCCACGAAAGCAAAGGATCGACTAGGCTATGCTCCTGTGGTCCCACTTCAGGAAGGTATACGGA GGACGATAGACTCATTCTCTCACCTAACAGCTGGAAGTCAATCCAAAAGAGAGGGCCCATCCAAGGCTTCTAGGATTCTT GGAGGCGGAAAGGTTGCTGATACACTTCTCTGGAAGGATTTAAAGCAGACTCTTATTGCCATATTCATTCTAATTAGTAT CTATTATAACTTTGTTGCAACTGGGTCTACCGTCGTAACTGCTCTTTCGAAGGCTCTATTGGTAGCATCTGTATTTTTGT TCCTCCATGGCATTCTACCAGAGAAAATATTTGGTTATACAGTTGAAAAGATTCCTGCATCGCAATTTCACCTCTCAAAA GACTCATCACATGACCTCTCATTGTCAGTGATCTCTTCATGGAACACCACTGTAAAAGCTTTAAGATCTCTTTGCCAGGG GAATGATTGGAGCTTCTTTTTCAAGGTGGTCTTCGTTTTGTTAGCATTGAGTTTGGCTGGAGCCATATCTCTTCACAGCA TATTTGTTATAGGACTGCCCATTGCCTTCCTTGCTTTTCTCGTGTACGAAAAGAAAGAGCAAGAGATCGACTCAATAGTG GTGAGTTTTAAATCTTTCGCATGCAAGCACAAATCCGATGTGTATGAGAAATTGTTCGGAAGCAAGAAACATGACTAAAA ACCCTTTTTTTGCCCAGCATTTGTAATTTTCTTTTGTTTCCAACGACCTCTAACTCTGGTTTGAGCTTTTTTCAAGTTTT ATTATTTGTGAATCTCT ................................................................................ ....................................N........................................... ................i...................................................N........... ......N........N...........N..................................................N. .......N.........................i.....................N........................ ...................i.........N........N......................................... ...........................................................................N.... ..........N....................N........N.....N................................. ..N...........................................N..........................N...... .........N..............................................N..N.................... ............................N....i...........................................N.. ............................................N................................... ................................................................................ ................................................................................ ................................................................................ ......N......................................................................... ..........N...........................N......................................... ..N............................................................................. ................................................................................ ....................N................N.....N..........................N......... ................................................................................ ................. Pos Score Pred ------------------------ 117 0.319 - 177 0.765 Yes 229 0.127 - 247 0.343 - 256 0.095 - 268 0.196 - 319 0.114 - 328 0.164 - 354 0.740 Yes 376 0.202 - 420 0.671 Yes 430 0.207 - 439 0.298 - 556 0.207 - 571 0.179 - 592 0.231 - 601 0.108 - 607 0.109 - 643 0.140 - 687 0.335 - 714 0.379 - 730 0.148 - 777 0.336 - 780 0.288 - 829 0.389 - 834 0.684 Yes 878 0.360 - 925 0.488 - 1207 0.353 - 1291 0.433 - 1319 0.299 - 1363 0.141 - 1541 0.313 - 1558 0.457 - 1564 0.136 - 1591 0.459 -

Explain the output -- Processing locus 315: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGATTTGCTAATGTAGGTGTTACAACTTCTGTCAATATTTCATTTTGATTTTTTTAATTTTTCAATAGTATTTGTCCAC TATAATTAGTGTAAAAACTAAAAATCCCAAAAAGAGAGGAAGATAAATAATCATTTTAAGAAAAGAAAAAGAAGTTGGAG ATGGATACGGATGACATGTCAAGTCCGACAAGGAAATACATGCACTTGTCGTCCTTCTAATCCTTTGCCCTTAAACATCG TTAAAAACGACAACTTCTCTCTTCTTCTTCTCTACTCTTCTCCATTTTCTTCTCTTCTTCTCTTCTTTGTCCATTTTTTG ACAGATATGGCGATCTGGTTTGCTAGATCCAAAACCCTTGTTTCTAGCTTGAGACATAATCTAAATTTGTCGACAATTCT CATAAAACGTGATTACTCTCATCGTCCCATCTTCTATACAACTTCTCAGTTATCTTCAACGGCGTATTTGAGTCCCTTCG GTAGCCTCCGTCATGAGTCTACGGCCGTGGAGACACAGGCTGATCATTTGGTTCAGCAGATTGATGAAGTCGATGCCCAG GAACTGGATTTCCCAGGAGGCAAAGTCGGTTACACATCGGAGATGAAATTCATACCGGAATCATCTTCAAGGAGGATTCC ATGTTACCGGGTTCTTGACGAAGACGGACGAATCATCCCCGATAGCGATTTTATTCCGGTGAGTGAGAAACTCGCTGTTA GAATGTACGAACAAATGGCGACGCTACAAGTAATGGATCACATCTTCTACGAAGCTCAACGTCAAGGAAGAATATCTTTT TATCTTACTTCCGTCGGAGAAGAAGCCATTAACATCGCTTCAGCAGCTGCTCTCAGTCCTGACGACGTCGTTTTACCTCA GTACCGAGAACCTGGAGTTCTTTTGTGGCGTGGCTTCACGTTGGAGGAGTTTGCTAATCAGTGTTTTGGGAACAAAGCTG ATTATGGCAAAGGCAGACAAATGCCAATTCATTACGGTTCCAATCGTCTTAATTACTTCACTATCTCCTCTCCAATTGCC ACGCAACTTCCTCAAGCTGCTGGAGTTGGTTATTCTTTGAAAATGGACAAGAAGAATGCTTGTACTGTTACATTCATCGG AGATGGTGGCACAAGCGAGGGAGATTTTCACGCCGGATTGAATTTTGCGGCCGTAATGGAAGCTCCGGTTGTGTTTATAT GTCGGAACAACGGTTGGGCGATTAGTACTCATATCTCAGAACAGTTTAGAAGTGATGGAATAGTTGTGAAAGGTCAAGCT TACGGTATCCGAAGCATCCGTGTGGACGGTAACGATGCACTTGCGGTTTATAGTGCTGTACGCTCAGCTCGAGAAATGGC TGTAACAGAACAAAGACCTGTTCTCATTGAGATGATGACATATAGAGTAGGACATCATTCTACATCAGATGATTCAACTA AGTACAGGGCGGCGGATGAAATCCAGTACTGGAAAATGTCGAGAAACCCTGTGAATAGATTTCGGAAATGGGTCGAAGAT AACGGATGGTGGAGTGAGGAAGATGAATCCAAGCTAAGATCTAACGCAAGAAAACAGCTTCTGCAAGCGATTCAGGCTGC GGAGAAGTGGGAGAAACAACCATTGACAGAGTTGTTTAACGATGTATATGATGTTAAACCGAAGAACCTAGAAGAGCAAG AACTTGGTTTGAAGGAATTAGTAAAGAAACAACCTCAAGATTATCCTCCTGGCTTTCATGTTTGAATCTAGAGGAACTGT GTGGTTAAAATACCTCATATAGTATCATGTCCATATCGACTTCTCATTGCAGACTATTTATATTGTCCACGTATCGAATA GTAA N..........N.................................................................... ................................................................................ N.........N....N.......................N........................................ ................................................................................ ......i......................................................................... ................................................................................ ............N..................................................N........N....... ..........................................i..................................... N............................................................................... ..i...........i.................i............................................... ................................................................................ ................................................................................ ...N................i........................................................... ..........................................i............N........................ ..N....................................................i......................i. ......................................................N......................... ..................................N........................................i.... ...............................N..i.................................N........... ...............N...................i...............................i............ .....i................N......................................................... .........................................N.....N..N............................. .........................................................N...................... ..........................N..................................................... .... Pos Score Pred ------------------------ 1 0.196 - 12 0.445 - 161 0.401 - 171 0.171 - 176 0.203 - 200 0.141 - 327 0.699 Yes 493 0.280 - 544 0.155 - 553 0.201 - 603 0.588 Yes 641 0.405 - 723 0.743 Yes 735 0.733 Yes 753 0.535 Yes 964 0.451 - 981 0.648 Yes 1083 0.835 Yes 1096 0.257 - 1123 0.269 - 1176 0.700 Yes 1199 0.526 Yes 1255 0.386 - 1315 0.244 - 1356 0.868 Yes 1392 0.415 - 1395 0.556 Yes 1429 0.382 - 1456 0.339 - 1476 0.692 Yes 1508 0.760 Yes 1526 0.563 Yes 1543 0.166 - 1642 0.186 - 1648 0.161 - 1651 0.172 - 1738 0.194 - 1787 0.069 -

Explain the output -- Processing locus 316: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCAAAAGTGTAGTGCGAACCATTTGTGAAACAATCTCTTCAAGCTTCTTGTCTGCTCTTCATCTTTTTTTGTTGTTTTAA CCAAAGAAGTCTACAAAGTGAAGCTCTATTAATGGTCCTAGATCTAATTTTGCTTTTACTTTTCTCTTTTTGTTCGATAT AGACATTGTTACAAGTCCTATTCATCCACTTCTTCACTTTTTTTTTTGTTATCTTACTAAAGCATTGGTGATGAATCTGC AGATGGAGTCTGTGAAACCATTGGATGTTGTTTCTTCAGGAAAAGGAAAACTCAGACGCACATTTGCTAAAGTCATCAAC ATGAAAAAGCTAACCGGTGTTGTTCCAGAAGGTAACAACAAGGTTGAAAGAGTGAAGAAGAGCCAAGAAAAGGTGAAGTT GGATAAAGATTTAGCAAAGAATGCTGCAAATCTGTCTGAGTCCTTTGACAAGCTGGAGGAAGAGTATGAGAAAAGACTGG CCATGGAGGCTCTTCTTGCAAAGCTGTTTGCTACGATTTCTTCGATAAAATCTGGTTATGCGCAGTTGCAGTATGCTCAG TCTCCTTATGATCCAAATGGGATTCAGAAAGCAGATAACTTGGTAGTAGCAGAGCTGAAGACATTATCAGAGCTGAAACA GAGTTTCTTGAAGAAACAGCTTGATCCTAATCCTGATAGAACTCTTGTTCTTGCGGAGATTCAAGAGCTAAGAAGCGTCT TAAAGACTTATGAGATCATGGGGAAGAAGCTGGAGTGTCAGTTAAAGCTCAAAGACTCTGAGATCATATTTCTCAAAGAA AAGTTTCAAGAATCCATGACTCAGAACAAGTTGATGGAGAAGAGATTAAACCAAAGTGGCCAACTTTGCAATCCATTGGA TCATAATCTTCATCTATCGGCGGTAAGTTCGACCCATTTCGTTACTTATCTGCACCACACTGTCAAATCCATCCGAGGAT TCGTCAAGTTGATGGTTGAGCAGATGAAACTCGCTGCTTGGGACATCGATATGGCTGCTGAGTTAATTCAGCCTGATGTA CTCTATTACAAGCAAGACCACAAGTGTTTTGCCTTGGAGCATTATGTCTGCAAGATAATGCTTGAAGCTTTTCAACTACC TTACTTCTCAAACGAATCATCGAAGAAGACGAGCAGAGAAGACAAAGCAATGTTCTTTGAGAGATTCACAGAGCTTAGAT CGATGAAACCAAGAGAGTATTTAGCATCTCGGCCTAAATCAAGATTGGCTAAGTTTTGCAGAACTAAGTATTTGCAGCTC ATACATCCAAAGATGGAGCAGGCATTCTTCGGACATTTGCATCAAAGAAACCAAGTCACTGCAGGGGAGTTTCCAGAGAC GAGCTTGTGCACTGCGTTTCTCGAAATGGCGAAAAGGGTTTGGCTTTTACATTGCCTTGCTTTCTCCTTCGATCCTGAAG CTTCAATCTTTCAAGTATCTAGAGGTTGCAGATTCTCCGAAGTGTACATGAAGAGCGTGTCGGAGGAAGCGTTTTTCTCA CCGGAGCAAGAAGAAAGCTCGTCGGAAACTGAACCGGGGGTTGCGTTTACGGTTGTTCCTGGGTTTAGAATTGGGAAAAC GACGATACAATGTGAGGTCTACCTCTCGCGTTCATGTCAACGCCGTCAGAGCAGATAGATTCCGGTGATGACGTCACAGG GGTATTAGGGTAATTTATCTTGGGGAACTTTTTGGGGGTAGGAAAATATATGAGAGAAAATTTGTTGACCGTTGGAT ................................................................................ ...............................N................................................ ......................................................................i......... ..i.....................N....................................................... i............................................................................... ....................N............................................N.............. ..N......................................................N..............N....... .......N........N............................................................... ................................................................................ .........N.......N.............................................................. ...............N.................i.............................................. ................................................................................ ...........i...........i..........................N........................N.... ...........................................N.............i...................... .................................................i.............................. ..N............................................................................. ............i................................................................... .........................i...................................................... ...............................................N................................ ................................................................................ .........i.......................N.................................N............ .................................................N........................... Pos Score Pred ------------------------ 112 0.171 - 231 0.626 Yes 243 0.693 Yes 265 0.209 - 321 0.739 Yes 421 0.067 - 466 0.082 - 483 0.459 - 538 0.189 - 553 0.153 - 568 0.101 - 577 0.323 - 730 0.307 - 738 0.288 - 816 0.348 - 834 0.504 Yes 972 0.662 Yes 984 0.603 Yes 1011 0.432 - 1036 0.218 - 1084 0.373 - 1098 0.512 Yes 1170 0.616 Yes 1203 0.259 - 1293 0.675 Yes 1386 0.815 Yes 1488 0.500 - 1610 0.626 Yes 1634 0.254 - 1668 0.056 - 1730 0.053 -

Explain the output -- Processing locus 317: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTAATTGTGATTCTCGTGCATATGCGTGTGGTGTCATCTTTAGTTATGTAATTCGCGATTAGCTCTAAGATGGGTATTG AAAAACTTGCCTGATTCGTGATCGCTTCTGCTCATTTCTGTAAAAGTCAGAGTTTTGTTCATAAAGTTTGGGTCTTTAAG AAGAATAACAATTTTGTGCATGTTTTCTTCTTTAAAAGGGGAATTTCTAAAAATGGATATTGGAGAAATCATTGGTGAAG TAGCTCCTTCTGTGAGTATCCCAACGAAGAGTGCTATATACGTTTGGGGTTACAATCAAAGCGGACAAACTGGTAGGAAT GAACAAGAGAAGCTTTTGCGTATCCCAAAACAGCTTCCTCCTGAGCTTTTTGGTTGCCCCGCTGGTGCCAATTCGCGTTG GCTCGATATTTCTTGTGGTCGGGAACATACAGCTGCGGTTGCATCAGATGGTTCTCTCTTTGCTTGGGGTGCTAATGAAT ATGGTCAGCTTGGGGATGGAACCGAGGTTGGGAGGAAACATCCAAAGAAGGTGAAACAGCTGCAATCAGAATTTGTGAAA TTTGTGTCTTGTGGAGCTTTTTGCACTGCAGCTATCGCAGAACCCCGTGAAAATGACGGTACTCTTTCCACTAGTAGACT CTGGGTTTGGGGCCAAAATCAGGGATCAAATCTACCACGCTTGTTTTCGGGGGCATTTCCTGCTACAACGGCAATCCGCC AAGTATCATGTGGGACAGCTCATGTTGTGGCCTTATCAGAGGAAGGCCTTCTTCAAGCTTGGGGCTATAATGAACAAGGC CAACTTGGTAGAGGAGTCACTTGTGAAGGACTACAAGCACCTCGTGTGATAAATGCTTATGCGAAGTTCCTTGATGAAGC ACCCGAGCTTGTGAAGATTATGCAACTTTCATGTGGAGAATACCATACTGCTGCTCTTTCTGATGCAGGCGAGGTTTACA CTTGGGGATTAGGAAGCATGGGTCAACTTGGGCATGTTTCTCTTCAATCCGGGGATAAGGAGTTAATACCAAGGAGAGTC GTTGGTCTCGATGGTGTGTCCATGAAAGAAGTTGCTTGTGGTGGTGTACACACTTGTGCTTTATCTTTGGAAGGAGCACT TTATGCTTGGGGTGGTGGCCAAGCAGGACAGCTAGGACTTGGTCCTCAATCTGGTTTCTTTTTTAGTGTCTCTAATGGAA GCGAAATGCTTCTACGAAATGTCCCGGTTTTAGTCATCCCAACTGATGTCAGGCTTGTTGCGTGTGGACATTCTCACACT CTCGTTTATATGAGAGAGGGACGAATTTGTGGATGGGGTTATAATAGTTATGGTCAAGCAGCAAATGAGAAATCGTCATA TGCTTGGTACCCATCGCCTGTAGACTGGTGTGTAGGACAAGTGAGGAAGCTAGCAGCAGGAGGTGGTCATTCAGCTGTTT TAACCGATGCATTTTCGTTGAAGGAGTTGTGTGAATTTCAATTAGCAGATAGTGTGAACCTCTCAAATGCTTCAGAGATT CAAGATGTTGCATTCAGAATGGGATCCGAAGCTTTAGCCCGCCTCTGCGAAAGACTGAGGGAGCAGTTACTTGATGGGGA CTTTACTAATGGGGAAGAAGTATATTAAAAAGCACAATCCCCAAACTCCGAAACAATTTAGTATTGTTGGAAGTTATTAC AATTGGTCCTATATTATTGAGAAATTGAAGAATGTAGCAAACCAGAA ......................N.......................N.......................N......... ................................................................................ ...................N................................i........................... ..............................................................................N. ................................................................................ ...............................................N..........................N..... N..............N................................................................ ....................................................i........................... ................................................................................ .......N.............i...............................................N.......... ....................................................N.....N..............N...... ...................N..........N...............................N................. .................i...............N.............................................. ..........i..........N.......................................................... ..N.......................................................................N..... .....i............N..........................i.................................. .........N......................N................N..............N..............N ................................................................................ ......N...........................................................i............. ....i.............i......................................................N...... ........i....................................................................... ...............................N............... Pos Score Pred ------------------------ 23 0.362 - 47 0.364 - 71 0.368 - 180 0.406 - 213 0.825 Yes 319 0.295 - 448 0.275 - 475 0.178 - 481 0.215 - 496 0.074 - 613 0.659 Yes 728 0.338 - 742 0.579 Yes 790 0.380 - 853 0.428 - 859 0.366 - 874 0.097 - 900 0.362 - 911 0.228 - 943 0.367 - 978 0.564 Yes 994 0.072 - 1051 0.581 Yes 1062 0.398 - 1123 0.265 - 1195 0.221 - 1206 0.528 Yes 1219 0.364 - 1246 0.511 Yes 1290 0.239 - 1313 0.430 - 1330 0.354 - 1345 0.138 - 1360 0.344 - 1447 0.195 - 1507 0.573 Yes 1525 0.535 Yes 1539 0.635 Yes 1594 0.234 - 1609 0.570 Yes 1712 0.060 -

Explain the output -- Processing locus 318: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAGGTTTTATTCATTTGGATGTTAGCTTCGGATCTGATATATATTTGATCTCTGGTAGCTTAATTTTACTAATCTACTG ATTTAGAGTTGTTGAATCTAAGACTTCAAATTTTGTTGCACTAGAGTTATGTTTTGGATCTACAGAAGTTGGGTTTTGTA TATTTACAGTGTTTTAGAAGACATAAAGAGGATAATGAAGAAGAACAACAAATACAATAAGAAATCAACATCTTTGCATT GCAATGATGCAGGAGGTTGCAGATATTCATTGCTTACTATTGTTTGGACCGTCGTCGGGTTTTTCCTGGTGGCTCATCTT ATCTCTCTGTATAGCAGAAAAGATAACAACATCCATCAACAAGTTTCTTCAGATCAGTTACAAGTAGTACATCATCTTGC TCATCCTATTGTTCGTGAACTTATAAGAGTTGAAGAAGAGGTCCTGAGAATGCCGCCACCTAGGAAGCGTTCTCCTCGTA CTAGTAAACGGAGATCAAGAAAACCGATCCCTCTCGTTGAGGAGTTTCTTGACGACAAGTCACCAATTCGACATCTTTTC TTTCCCGGTATCAAAACCGCCGCTTTTGGTCCCACCAAGGATATGGGAAACGAGACATCGTATTACTTCCCCGGGAAAAT TTGGATGGACACACAAGGGAATCCAATTCAAGCACATGGCGGAGGGATTCTGCTTGACGTGAAATCTAACACTTACTATT GGTATGGAGAGTATAAAGATGGACCAACTTACCATGCTCACAAGAAAGGACCAGCTAGAGTTGATATTATTGGTGTTGGT TGTTACTCTTCAAAGGACTTATGGACATGGAAAAATGAAGGCATTGTACTCGGAGCTGAGGAAACAAACAAGACCCATGA TTTGCATAAATCCAATGTTCTTGAACGACCCAAAGTGATCTACAATGAAAAGACAGAAAAGTATGTGATGTGGATGCATA TCGACGATGCAAACTACACTAAAGCTTCAGTTGGTGTGGCCATTAGCAATAGCCCGACAGGTCCTTTCGAGTATCTATAC AGTAAACGTCCACACGGGTTCGATAGTCGGGACATGACTGTCTTCAAAGACGACGATGGTGTTGCTTACCTGATATATTC GTCTGAAGTCAATAGTGTGCTTCATATTGGACCCTTGACGGAAGATTACCTCGATGTGACACCGGTTATGAAGAGAGTAA TGGTGGGACAACACAGGGAAGCTCCAGCTATCTTCAAGCATCAGAATATTTATTACATGGTCACTTCCTGGTGCACTGGT TGGGCACCAAATGAAGCCTTGGCTCATGCGGCTGAATCTATAATGGGTCCATGGGAGAAATTGGGGAACCCGTGTATCGG TGGTAACAAAGTTTTCCGGTTAACAACATTCTTTGCGCAGAGCACTTATGTGATTCCTTTACCTGGTGTTCCCGGTGCGT TTATATTCATGGCAGATAGGTGGAACCCGGCTGATTTGCGGGATTCCAGATACGTGTGGTTACCGTTAGTTATAGGTGGA CCTGCTGACCAGCCTTTGGAGTTCAACTTCGGGTTCCCATCGTGGTCCAGAGTGTCCATCTACTGGCACAGTAAATGGCG GCTGCCTTAAAGACATACAAATAACCAAATATGTTCTTACCGATCCGTCAGTGCTGATCATTGTTGTTGTCTTGTTGATT TGTTTTGTAGATTAGGCATTCATACACAC ...................N............................................................ ................................................N............................... ..................................i............................................. ...N..N......................................................................... ................................................................................ .................................................i.............................. ................................................................................ ..........................................i..................................... ....i..............................i............................................ ...N..............i..............N.............................................. ....................i.....N.......N.........................................N... ..............N.............................N.................N....i.....N...... ......i......................................................................... .................................i.....................N........................ .....................................................N.............N...........i ........................................................i....................... ..........N..............N................N.......N............................. ...............................................N................................ ........i....................................................................... ..........................................................................N..... ..............................N................................................. ............................. Pos Score Pred ------------------------ 20 0.135 - 129 0.245 - 195 0.509 Yes 244 0.155 - 247 0.316 - 450 0.596 Yes 603 0.766 Yes 645 0.591 Yes 676 0.701 Yes 724 0.270 - 739 0.516 Yes 754 0.340 - 821 0.547 Yes 827 0.341 - 835 0.343 - 877 0.398 - 895 0.169 - 925 0.357 - 943 0.347 - 948 0.719 Yes 954 0.310 - 967 0.550 Yes 1074 0.768 Yes 1096 0.216 - 1174 0.140 - 1188 0.284 - 1200 0.675 Yes 1257 0.514 Yes 1291 0.168 - 1306 0.342 - 1323 0.376 - 1331 0.363 - 1408 0.322 - 1449 0.607 Yes 1595 0.265 - 1631 0.082 -

Explain the output -- Processing locus 319: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTGTTTGGATTAAGAAATTGAAAATTAGTTTTGTAGCTGAGCCTTTTCTCTTGTGTGTATAGGAACTGAGCTATATCAG TAGTGTATCTAGCATATTTGATTAATGTTCCTTCATGTTGTTCTTATTGAAGCTGCTTCCATTGGTTTTGTGATTGAGTG CAGGTCTTATAACATGGACTCCATGGATATTGATCAAAGTAACATAGGTGAATCGCCTCACCTTCTGCTGAGACCAGTAA GTCCATTGGAATCTGGTGAAGGTTTACCTTATGCTCCTGAGAATTGGCCTAATCCTGGAGATACTTGGCATTGGAAGGTT GGACCGAGAATTAGCGGTAAAGGCTACTTCGTGGATAGATACTTATATCCTCCTAAGTATCTTCCCGGTTTAGATACTGA AATATTGCGGAAAAATAAGGTGTTTAGGAGCAGGCTCTCGCTCCAACGATATATTCGTGTACACTTCCCTGAAGCGGATG TTCAAAAGTTCTTTGCTTCTTTCAGCTGGAGCATACCCTGCAGAGATGGTCAAGGTGTTTTGCCTCAGAAGCAGGTTCAG TTACCAGTGTATAGCTCAGATGAAGATCCAATGCGTGATGATGGATCTGATACTGCGGTCTGTAAGGCTGGTAATGAAAA ATGCCGGAGCCTGATGCCACAGTGCGAAGCCGAGACTTTACCTGCTATGCCTTGTGACATTTGCTGCGGTGAACGTAAGT TCTGCGTTGATTGCTGTTGCATCCTTTGCTGCAAGTTAATAAGTTTGGAGCATGGAGGCTATAGCTATATCAAATGTGAA GCTGTGGTTTCCGAGGGTCACATATGTGGACATGTTGCTCATATGAACTGTGCTCTTCGAGCTTATCTGGCTGGAACCAT TGGAGGCAGCATGGGATTGGATACTGAATACTACTGCAGGCGATGTGACGCCAAAAAGGACTTGTTTCCACATGTCAACA AGTTTCTAGAAATATGTCAGACTGTAGAATATCAAGGCGACGTGGAGAAGATCCTGAATCTCGGTATTTGTATCTTGCGG GGAGCACAAAGAGACAATGCTAAGGAGCTATTAAATTGTATCGAATCAACAGTCATCAAGCTTAAATGTGGCACGAGCTT GGAAGATCTCTGGAATGATGATACACCAACTATATGGTCAGATTACTCCGACAGTGGAGAAGCTAGAGAAAATGATACAT TGCAAAGTTTACAGGACGTGACTCCAATTGGGCCCATACCATTCAACCATGAGGCTGAGATGCACAAACTCGAAGAAGAA ATCGGCGAGGTTCTAAGAGCGCTGAGAAAGGCTCAGGAATTTGAGTACCAAATTGCAGAAGGCAAACTTCATGCTCAGAA AGAATGTCTGAGTGATCTGTATAGGCAGCTCGAGAAGGAGAAGTCAGAGCTGTCGAGGCGAGTATCAGGTACAGATGCGA ATAGCTTGATGACGAATGTGCTGAAGAGATTGGATCAGATTAGAAAAGAAGTCACAAAGCTTAAAGAAATGGAAGAAGTA GCCAAGGGATTTGGTAGGACACCTAGAGGGGTTCTTGAAGAGTACTTTCATCTAAACATCGAGGACTAAGATTTATATAA GCACATCCATAATTGTACATTCGTTATGACGTTTGTTTTGTAAGTTTTTGATTCTTCCTCGTAAATCTGAGTTTTGGATG GTCATTTG ................................................................................ ........................N.........N............................................. .............N........i......................................................... ..............................N................................................. ................................................................................ .............................................................................N.. .............................................N.................................. ...................N..........i......N..N................................N...... i............N................................N................................. ...................................................i.....................N...... .......................N.......N..........N..................................... ..........N...............................N............................N........ .............i.................................................................. ................N................................................i.............. ..............N..N...............N.....................................N........ ................................................N..........i.................... ......................................................................N......... ...i......................................................................N..... ........N......N....................................................i........... ................................................................................ .........................N...................................................N.. ........ Pos Score Pred ------------------------ 105 0.246 - 115 0.464 - 174 0.407 - 183 0.567 Yes 271 0.253 - 478 0.279 - 526 0.435 - 580 0.390 - 591 0.697 Yes 598 0.260 - 601 0.307 - 634 0.364 - 641 0.765 Yes 654 0.305 - 687 0.345 - 772 0.512 Yes 794 0.132 - 824 0.365 - 832 0.492 - 843 0.467 - 891 0.336 - 923 0.290 - 952 0.105 - 974 0.647 Yes 1057 0.210 - 1106 0.534 Yes 1135 0.337 - 1138 0.268 - 1154 0.393 - 1192 0.247 - 1249 0.325 - 1260 0.551 Yes 1351 0.156 - 1364 0.656 Yes 1435 0.150 - 1449 0.465 - 1456 0.212 - 1509 0.557 Yes 1626 0.165 - 1678 0.034 -

Explain the output -- Processing locus 320: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAATCCCACCAAAATCGATTTAACACTAGTTTTGGTTTATTGGACCAAACCAAGTAGAAAACCAACAAAACCACCAGTAC CGTGATTATTTTCTTTTCTAACGCGTAATAAATATCTTTTATGGCTTTATTATGCACTCTCATTGCGAAAAAAGAAAAAT CCAAAGGCTTGGAAAATCGACAAAAACGAAAAGGGTAGTGGTGAGATGTTTCATCATTCTCTGTTTCAGTTCGGTAATGG CGGAGATTTGCTATTTTGGGGGTCTAGTCCATTATAAATCACCATCTCTTCCTTTGTCTCTTCATTCTCTCTTCCGCAGC AAGAAGCCACATTACTACAATTCCACTTCTCTCATGGCTGAATGTCTCTCCTCCTCCACCGTGGAGCCTCCTTCTCTCCT CGTCTTCTCAGGTGGTACTGGATTTAATGGTGTGGTTGAAGAGTTGAAGAAGTTAACAACACGAGTCGCTCATGTTCTTC CTGTCTCTGATGATGGTGGAAGTACTGCTGAGATTGTTCGTGTTCTCGGAGGGCCAGCGGTTGGTGATATACGTTCGAGG TGTTTAAGGCTGTCTGATGAGAGTACTTCAGAGGCACTTGCTGTTAGGCGGTTGCTTGGCCATCGCTTACCTATTGATGC ACATCAGGCCAAGAAAGATTGGTACGATATTGTAGAAGGTAATCATTCTCTGTGGGATGGTGTATCAAGACCCTACAGTG AAACAATTCGCGCTTTCTTGATATACTTTCAGAATGAGATTCATAGGCGACCTAATGAGAGATTTTGCTTCAGTAATGGA AGCATTGGGAACTTCTTCTTTGCAGGAGCAAGGATATTTTTCCAGTCTTTAGATGCTGCAATTTTTCTGTTTTCACGTGT TTCAGAGATTCCTTGTGACAGTTTGGTCCTCCCAGTGATATCAACCAATGATAGGCTAACACTAGGATGTGAATTACAGG ATGGGACTATAATACGCGGACAGAACGAAATTTCTCACCCGACCACTGGGACTACGCAGACAGTTGACAAGAGACATTGT TCTACTTCCGCTCTCCCATCGAAGATAAAGAGAGTGTTTTACATGTCAAGTGAAGGCAATAATCTGCTTCATGAGGTCTT CCCACCAGTTAACCCAACTGTCCTAGAGCAGTTGAGAAGCGTGGACTGTATCGTTTACGCAATGGGATCCCTGTTCACTT CCATTTGCCCATCACTGGTTTTGCTTGGTGTTGGGGAGATTATCTCTTCCAGGTCATGTCGTAAGGTACTTCTTTTGAAC GGTAGCCAAGACCGAGAAACAAGCAGGTTCACCGCTTCCTGCTTTGTGACTGCTATCGCTGACGCTCTCAACAGAACTCA TGGAGATACTAATATTCGGCTCAAGAATCCCCCTGGATATTACATCAACACTCTCTTGGTCCCGAAAGATGGAGAAATAG CAGTAGATCTCAAACAATTGTCTGAACAAGGGATATATGATGTTGTCAGTATCTCTTTCCTTCACTTGTTTCCAAGTTTA TGTGCACAACAAACACTCACTGTTACCTCCACTCTATTGCAGAGAGTCGTGGAGAGTGTTGGTGACCCGAAGCACGGTAT CTTGTTCAACCCGAGCTCACTGATAAACACACTGGCCGGTTTGGTGGAGTAAATAGTGATTCTTTAGGCCAGAGCTAGTT TCTTCTTCTACGCAATTGGGACCATGATTTTTTTGTTACTGTTTAGTTTGATCCTTTAGTGAATTGATATG ................................................................................ ........................................N..........N............................ .............................................N..............................i... ................................................................................ .................................i.......N...................................... ..........................N............................................N........ .........N..N................................................................... ................N...........................................................N... ........................................................N....................... .................................N....................N....................N.... ....................................................N........................... ...............................................N..................i............. N............................................................................... ..........................................i...........................N......... .............................................................N.................. .......................................................i........................ ...............................................................................N ....................................................................i........... ....................................N..N.......................................i ................................................................................ ................................................................................ .......................N............................................N.. Pos Score Pred ------------------------ 121 0.393 - 132 0.262 - 206 0.196 - 237 0.559 Yes 354 0.710 Yes 362 0.414 - 427 0.290 - 472 0.128 - 490 0.215 - 493 0.211 - 577 0.180 - 637 0.278 - 697 0.182 - 754 0.282 - 775 0.204 - 796 0.293 - 853 0.156 - 928 0.429 - 947 0.530 Yes 961 0.347 - 1083 0.571 Yes 1111 0.276 - 1182 0.424 - 1256 0.622 Yes 1360 0.115 - 1429 0.550 Yes 1477 0.493 - 1480 0.342 - 1520 0.552 Yes 1704 0.044 - 1749 0.052 -

Explain the output -- Processing locus 321: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGGAGAAAGTTATGTCCAACAAAAAAGAGAGAAAAAAATGCAATTAGCCTTTTAATAAAAAAAGGAGTAGTAATTACA AAAAAAAATGAAGTACTGTAGATTATGATGAGTAGGGAGGAGGAAAACGAGTATGATACTTCACCCGCATTAAGAGGCAA ACCCTTAAAGCCTGCTGATTGGTTGGGACTTTTTTTTCTACTTTTCTTTTTCTTTTCTGTTGCTGCCGTTACAGTTCCAT TTACAGCTCATATTGCTTGCGGCAGAAAATTCTCACCAAGTCCTCTGTTGAACATGACGATTAAGCCTAAATCGCCGGCG AGATTCAAGCTAGGTAGGCAGTCTTCGCTTGCGCCGGAATCAAGAACCCCGATTGATACGTTAACGGAAGATGAGGACGA CGACTTGGCTGCGGCGGCTACAGCTGGGATCGGGGATCCGACGATTCGTTTAATGTATTTAGCCAACGAAGGTGATATCG ATGGCATCAATAAGATGCTTGATTCGGGTACTAATGTTGATTACCGTGACATCGATGCCCGTACTGCTCTTCACGTCGCC GCTTGTCAAGGACGAACCGATGTCGTTGAGCTGCTGCTTAGCCGTGGTGCTAAGGTTGATACGAAGGACCGATGGGGTAG TACGCCTCTTGCAGATGCAGTGTATTACAAAAATCATGATGTGATTAAACTTTTGGAGAAACATGGTGCTAAGCCTACGA TTGCTCCTATGCATGTCCTAACTGATAAAGAAGTTCCAGAGTACGAGATTCATCCTACAGAGCTTGATTTTTCCAACTCT GTCAAAATTTCAAAGGGTACCTTTAACAAGGCTTCATGGCGTGGAATTGACGTGGCTGTTAAAACCTTTGGAGAGGAAAT GTTCACTGATGAAGACAAAGTGAATGCATTTAGGGACGAACTTGCACTGCTTCAAAAGATACGCCATCCGAATGTTGTTC AGTTTCTAGGGGCAGTTACTCAGAGTACCCCCATGATGATTGTCACCGAGTATTTACCAAAGGGAGATCTTCGACAATAT CTAGACAGGAAAGGACCTCTAATGCCAGCACACGCAGTGAAGTTCGCCCTTGAAATTGCTAGGGGAATGAATTATTTGCA CGAACATAAACCTGAAGCAATAATCCATTGCGACCTAGAACCTCCAAACATACTGCGGGATGATTCGGGACATCTGAAAG TTGCAGACTTTGGAGTCAGCAAGCTGCTGGTAGTTAAGAAGACAGTTAAAAAAGACAGACCTGTTGTTACATGCTTGGAC AGTTCATGGCGATATATGGCTCCAGAGGTTTATAGGAATGAAGAGTATGATACAAAAGTAGATGTATTCTCTTTCGCTTT AATCTTACAAGAGATGATAGAAGGTTGTGAACCATTTCATGAGATAGAAGACCGCGAAGTTCCTAAAGCATATATTGAAG ATGAACGTCCACCATTCAATGCTCCAACAAAATCATATCCTTTCGGGTTACAAGAGCTAATCCAGGATTGTTGGGACAAA GAAGCATCAAAAAGACCAACATTTAGAGTAATCATCTCTACTTTGGAGTTGATAAGTGATCGAATTGCACGCAAAAGGAG CTGGAAGGTGATGCTAGGAAGATGCCTTCCAAGATTCAGATTGTTTACAAAGCGAGATTATGTGAATCCCGGTGGTAGTA ACCGCTCATCAGGCTCATTCAACAGATGAGTGACTGCAAAGAGCTTTGGGTTTGTTTTTCTTTAATTGCCTTAAAAGGAT GAAAAAATGTCAAATACTTTTTAGGTCTTTTGTATTTATATATGACAT .............N.........................N........................................ .......N................N..N........................N........................... ................................................................................ .....................................................i.......................... ......................................................................N......... ....................................................N........................... N.............i..................i....................N......................... ...................N...................................................i........ ..............N....................N..N.......................N................. ........i...N................................................................... ...................................N..........................................i. ........N..............N...............................................N........ ................................N..N............................................ .....................i............................................N............. ...........................................................N.................... ......................................................................N......... .....N.........N.....................N........N..............N.................. .............i........................N......................................... N.................N............................................................. ................................................................................ ..........N..........i.....................................N.................... .........................N....................................................N. ......N..................................N...... Pos Score Pred ------------------------ 14 0.461 - 40 0.247 - 88 0.386 - 105 0.127 - 108 0.197 - 133 0.151 - 294 0.544 Yes 391 0.265 - 453 0.421 - 481 0.369 - 495 0.848 Yes 514 0.511 Yes 535 0.167 - 580 0.402 - 632 0.656 Yes 655 0.080 - 676 0.161 - 679 0.135 - 703 0.105 - 729 0.593 Yes 733 0.141 - 836 0.493 - 879 0.596 Yes 889 0.251 - 904 0.185 - 952 0.263 - 993 0.418 - 996 0.423 - 1062 0.840 Yes 1107 0.267 - 1180 0.289 - 1271 0.132 - 1286 0.269 - 1296 0.388 - 1318 0.196 - 1327 0.156 - 1342 0.269 - 1374 0.558 Yes 1399 0.251 - 1441 0.354 - 1459 0.200 - 1611 0.104 - 1622 0.662 Yes 1660 0.349 - 1706 0.141 - 1759 0.076 - 1767 0.127 - 1802 0.048 -

Explain the output -- Processing locus 322: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCATTGACAAATAAACAGACACGTGTCATGTTTTGATAGTGAATATTTCTTTGTCTAAAAAACATATGTTAGTGGAAT TCAAAAGCGAAGACCAATTAAGTAAACGGAGCCGCCATTTCTTACTTCCACCACATGTTTCTCTTCTTCTTCATCTTTAC TATTATTCTAGATCATCATTATATGAATATATATACGTACACAAAATCATACGTTTTTCCACAAATGGATCAAGAAGAGG AAGAGATCGAGATTCCTAATTACTTTATCTGCCCAATCTCACTAGAGATCATGAAAGATCCAGTCACGACCGTTTCTGGC ATCACTTACGACCGTCAAAACATCGTTAAATGGCTCGAAAAGGTCCCTTCTTGTCCCGTCACGAAGCAACCTCTTCCCTT AGACTCCGATCTTACACCTAACCACATGCTTCGCCGTCTTATCCAACACTGGTGCGTCGAGAACGAGACACGTGGCGTTG TCAGAATCTCTACACCTCGAGTTCCTCCTGGAAAACTTAACGTCGTCGAAGAGATCAAGAATCTCAAAAAGTTCGGCCAA GAAGCCTTAGGAAGAGAAGAAACGTTGCAGAAGCTTGAAGTTTTGGCAATGGACGGAAATAACAGAAGATTGATGTGTGA ATGTGGCGTACATAAGTCTTTGATCTTGTTCGTAGTCAAGTGTACTAGTGAAGACGAAGACGGACGACGTCGTATCAAAG GACTAGATGAGTCTCTTCGTCTTCTTCACTTGATCGGTATTCCATCAAACGACGCAAAAACAATCTTGATGGAAAACGAT CGAGTTATGGAATCGTTGACTTGGGTTTTACACCAAGAAGACTTTCTCAGTAAGGCTTATACAATTGTGCTATTGAGGAA TCTAACGGAATACACTTCCTCTCATATCGTAGAGAGATTGAATCCCGAGATTTTTAAAGGGATCATAGGGTTTCTCAAGG ATGTTGTAAATAGTGTTAATCGGACGAGCCCTACCGTGAGAGAAACGGTACAGTCATCATCAAGGCCTAGCTTGGGAAAA ACGGAACCATCAAAACTTGATCATAGTTTGGTCATCAAACAAGCGGTGACTGCCGCGCTGATGATTCTTTTAGAGACTTC TTCGTGGAGCCGAAACCGATCACTCCTTGTGGATCTCGGTGCGGTTTCTGAACTCATCGAACTCGAAATAAGCTACACTG GCGAGAAAAGAATCACAGAACTTATGTTAGGAGTTTTATCTCGTTTATGTTGCTGTGCGAACGGTAGAGCCGAGATTCTT GCTCATAGGGGCGGAATCGCGGTTGTGACAAAGCGGCTATTGAGGGTTTCACCTGCGGCAGACGATAGAGCTATCTCTAT CCTAACTACAGTGTCAAAATTCTCGCCGGAAAACATGGTGGTGGAAGAGATGGTTAATGTTGGGACGGTAGAGAAGCTCT GTTCCGTTCTAGGAATGGATTGTGGTTTGAACTTGAAGGAGAAAGCAAAAGAGATACTAAAAGATCATTTTGATGAGTGG AAGAAGTTTCCATGTATTGACATAACTCTTCTCACTAAGCTGCTAAGCATCTCACCCAAAGGGCCAAAGATCTAAGTAAA TAATCTGCTGCACACACAACGCATACTTAGCATCGAACTTTTTTCATACTCAAACACATAGATATCGAAATATTCAAGTG TATGCATTTTTTTT .............................i.....................................N............ ......................................................N......................... ......................N.........................................i............... ..................................................i............................. .............................i.................................................. .........................N...................................................... ................................................................................ ................................................i.......................N....... N............................................................................... ......N.............................................................i........... ......i......................................................................... ................................................................................ N............................................................................... ............................................................i................... ................................................................................ .......................i......................N................................. ................................................................................ ..................................i..............N......N....................... ..............N.........................................................N....... ...........i.................................................................... ................................................................................ Pos Score Pred ------------------------ 30 0.571 Yes 68 0.315 - 135 0.313 - 183 0.391 - 225 0.764 Yes 291 0.626 Yes 350 0.667 Yes 426 0.469 - 609 0.550 Yes 633 0.468 - 641 0.191 - 727 0.232 - 789 0.701 Yes 807 0.546 Yes 961 0.206 - 1101 0.512 Yes 1224 0.584 Yes 1247 0.186 - 1395 0.766 Yes 1410 0.436 - 1417 0.167 - 1455 0.326 - 1513 0.228 - 1532 0.764 Yes 1682 0.045 -

Explain the output -- Processing locus 323: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATTTAGTTATTGAAGCAATCTATATTAGTAGTAAATATATACAAATTTTATGTTAAGCTCAAATAATTTCTTTAGTAC CAACGTTTGATTTCCCTATGATCAGAAATTTTCCACATTATTTTTACTCAAAAATATAAATATCTAAAATATAGAAACTC CCCAGTCACAAAAGAAGCCGTGTTGGAGCTAGAAGCCACGGGAAACCTCGTACTCTCCGACCAAAACACTGTCGTCTGGA CCTCAAACACGTCAAACCATGGTGTTGAATCAGCGGTTATGTCCGAATCTGGAAACTTCCTCCTCCTTGGAACAGAAGTT ACTGCTGGTCCAACCATTTGGCAAAGCTTTTCGCAACCTTCCGACACTCTCCTCCCAAACCAACCCTTAACCGTTTCCTT AGAACTAACCTCTAACCCTTCACCGTCGCGCCATGGCCATTACTCCCTGAAAATGCTGCAGCAACACACTTCACTTAGCC TCGGCCTAACCTACAACATCAATCTTGACCCTCACGCAAACTACTCGTACTGGTCCGGACCAGATATATCTAATGTTACA GGAGATGTTACCGCAGTTCTTGACGATACCGGAAGCTTCAAGATCGTTTATGGAGAATCCTCAATAGGAGCAGTGTACGT CTACAAGAACCCGGTAGATGATAACCGGAATTACAACAACAGTAGTAATTTAGGGTTAACAAAAAATCCGGTTCTGCGGA GATTGGTATTAGAGAACAACGGTAATCTCCGGTTATACCGATGGGACAACGACATGAACGGTTCAAGCCAATGGGTCCCT GAATGGGCAGCTGTATCAAACCCGTGTGACATAGCTGGGATTTGCGGTAACGGAGTTTGCAATTTGGACCGAACCAAGAA AAACGCTGACTGTTTATGTTTGCCCGGTTCGGTCAAACTTCCTGATCAAGAAAACGCTAAACTCTGTTCAGACAACTCAT CTTTGGTCCAAGAATGTGAAAGCAACATTAATCGTAACGGTAGCTTCAAGATCTCGACGGTCCAAGAGACCAACTACTAT TTTTCAGAACGTTCTGTCATCGAAAATATCAGCGATATCAGCAACGTGAGGAAATGCGGTGAGATGTGTTTGTCAGATTG CAAGTGTGTAGCTTCAGTTTATGGTTTAGATGATGAGAAGCCTTATTGTTGGATTTTAAAGAGTCTGAATTTTGGCGGGT TTCGAGATCCTGGCTCAACCCTTTTCGTGAAGACTAGAGCTAATGAATCTTATCCCTCAAATTCGAATAATAATGATTCT AAATCGCGTAAGAGCCACGGATTAAGACAAAAGGTTCTGGTGATTCCTATAGTTGTGGGGATGCTTGTGCTTGTGGCACT ACTTGGGATGTTGTTATACTATAATTTAGATAGGAAGAGAACACTAAAGAGAGCCGCAAAGAACTCTCTTATCCTTTGTG ACTCTCCTGTGAGTTTCACTTACCGCGATCTCCAGAACTGTACCAACAACTTCTCCCAACTTCTTGGATCAGGTGGATTT GGGACAGTATACAAAGGAACAGTAGCGGGTGAAACGCTAGTCGCGGTGAAGAGATTAGACAGAGCATTATCTCATGGCGA GAGAGAGTTCATCACTGAAGTCAATACCATTGGTTCAATGCATCACATGAACCTTGTTCGCTTGTGTGGTTACTGCTCGG AAGACTCACACCGGCTTCTAGTTTATGAGTACATGATAAATGGGTCGTTAGACAAATGGATATTCTCTTCAGAACAGACA GCTAATCTACTTGATTGGCGAACACGTTTTGAAATAGCGGTTGCGACTGCACAAGGAATCGCATATTTTCATGAGCAGTG TCGAAACAGGATTATACATTGCGACATTAAACCTGAAAACATCTTGTTGGATGATAATTTTTGTCCTAAGGTATCAGATT TTGGGCTAGCCAAGATGATGGGGAGAGAGCATTCGCATGTGGTTACGATGATTAGAGGGACGAGAGGGTATCTAGCGCCC GAATGGGTGAGTAACCGCCCGATCACGGTGAAGGCCGATGTGTATAGTTATGGAATGCTTCTTCTAGAGATCGTAGGTGG TAGGAGAAATCTTGATATGTCCTATGACGCTGAGGATTTCTTTTACCCTGGATGGGCCTACAAGGAACTAACGAATGGGA CATCTTTGAAAGCTGTGGATAAAAGGCTACAAGGAGTAGCAGAGGAAGAAGAAGTAGTGAAGGCTCTTAAAGTGGCTTTC TGGTGCATACAAGACGAAGTATCGATGAGGCCGTCGATGGGTGAGGTGGTTAAGCTTTTAGAAGGCACTTCGGATGAGAT AAATCTGCCACCGATGCCACAGACGATTCTAGAACTTATAGAGGAAGGATTGGAGGATGTGTATAGAGCGATGAGGAGAG AGTTCAATAATCAGCTTAGCTCTTTGACTGTTAATACAATCACAACCTCTCAGAGTTATCGTTCCTCCTCTCGGTCTCAT GCTACTTGTAGTTATTCTTCAATGTCTCCTAGGTAGTTTTCTTTTCTTTTTTGCATCATTTTATTGTTTGAGATCATTTC TCTATTTCTAGGACTAATCAAAAATATGATATTTATGTATGTTTAAGTCTCTTAC ...................................................N............................ .................N.............................................................. ................................................................................ ..................N...................i......................................... ................................................................................ ................................i...................i........................... ........................................................................i....... ....N............................................N.............................. .................N.............................................................. ........................................N............i................N......... ..N............................................................................. ...............N................................................................ .............i.................................................................. .....................................................N.........N................ ....................N........N..N............................................... ..........................................N.............................N....... ............................................................N................... .......N........................................................................ ................................................................................ .........................................................................i...... .....................................N........N................................. ........................N.......N......i...............i........................ ......................................................................N......... ..................................................N............................. ..............i..i..................N..........N................................ ..i..................................N...........i....N......................... ................i......N...........................N......................N..... ................................................................................ ........................N...........N....................................N...... .............N..........................................i.............N......... ..............................................................................N. .....................N.......................................................... .........................N........N...N................ Pos Score Pred ------------------------ 52 0.367 - 98 0.129 - 259 0.455 - 279 0.620 Yes 433 0.639 Yes 453 0.874 Yes 553 0.507 Yes 565 0.465 - 610 0.295 - 658 0.201 - 761 0.289 - 774 0.783 Yes 791 0.147 - 803 0.279 - 896 0.213 - 974 0.563 Yes 1094 0.301 - 1104 0.485 - 1141 0.137 - 1150 0.097 - 1153 0.089 - 1243 0.238 - 1273 0.051 - 1341 0.202 - 1368 0.283 - 1594 0.614 Yes 1638 0.216 - 1647 0.167 - 1705 0.146 - 1713 0.264 - 1720 0.547 Yes 1736 0.541 Yes 1831 0.252 - 1891 0.112 - 1935 0.722 Yes 1938 0.676 Yes 1957 0.153 - 1968 0.251 - 2003 0.547 Yes 2038 0.344 - 2050 0.563 Yes 2055 0.344 - 2097 0.562 Yes 2104 0.297 - 2132 0.405 - 2155 0.263 - 2265 0.488 - 2277 0.400 - 2314 0.137 - 2334 0.122 - 2377 0.685 Yes 2391 0.270 - 2479 0.071 - 2502 0.426 - 2586 0.042 - 2595 0.065 - 2599 0.079 -

Explain the output -- Processing locus 324: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACAACCGCAAGTTTGGATTTATACGATGAAGCGTTTATCTAGTGAGCGTCATCGCAGACGTCACAAGTTATGTGGTAC AATTTTGCGTATGTTTGATGAATACGCATGCATGTGCAGACATCAAAATCCAATTCCTCATCTATATTTATATATATGTA TATATATACATATGAAAGCACAGATATATCCACAAGGAGAGACACAGATTCTTGTATTGATACTCACTTCAGTGGAGCTA AGACAAGTAGAAAAAAAATGGCGCCACCGCATTTTCTACTGGTAACGTTTCCGGCGCAAGGTCACGTGAACCCATCTCTC CGTTTTGCTCGTCGGCTCATCAAAAGAACCGGCGCACGTGTCACTTTCGTCACTTGTGTCTCCGTCTTCCACAACTCCAT GATCGCAAACCACAACAAAGTCGAAAATCTCTCTTTCCTTACTTTCTCCGACGGTTTCGACGATGGAGGCATTTCCACCT ACGAAGACCGTCAGAAAAGGTCGGTGAATCTCAAGGTTAACGGCGATAAGGCACTATCGGATTTCATCGAAGCTACTAAG AATGGTGACTCTCCCGTGACTTGCTTGATCTACACGATTCTTCTCAATTGGGCTCCAAAAGTAGCACGTAGATTTCAACT TCCCTCCGCTCTTCTCTGGATCCAACCGGCTTTGGTTTTCAACATCTATTACACTCATTTCATGGGAAACAAGTCCGTTT TCGAGTTACCTAATCTGTCTTCTCTGGAAATCAGAGATCTTCCATCTTTCCTCACACCTTCCAACACAAACAAAGGCGCA TACGATGCGTTTCAAGAAATGATGGAGTTTCTCATAAAAGAAACCAAACCGAAAATTCTCATCAACACTTTCGATTCGCT GGAACCAGAGGCCTTAACGGCTTTCCCGAATATCGATATGGTGGCGGTTGGTCCTTTACTTCCCACGGAGATTTTCTCAG GAAGCACCAACAAATCAGTTAAAGATCAAAGTAGTAGTTATACACTTTGGCTAGACTCGAAAACAGAGTCCTCTGTTATT TACGTTTCCTTTGGAACAATGGTTGAGTTGTCCAAGAAACAGATAGAGGAACTAGCGAGAGCACTCATAGAAGGGAAACG ACCGTTTTTGTGGGTTATAACTGATAAATCCAACAGAGAAACGAAAACAGAAGGAGAAGAAGAGACAGAGATTGAGAAGA TAGCTGGATTCAGACACGAGCTTGAAGAGGTTGGGATGATTGTGTCGTGGTGTTCGCAGATAGAGGTTTTAAGTCACCGA GCCGTAGGTTGTTTTGTGACTCATTGTGGGTGGAGCTCGACGCTGGAGAGTTTGGTTCTTGGCGTTCCGGTTGTGGCGTT TCCGATGTGGTCGGATCAACCGACGAACGCGAAGCTACTGGAAGAAAGTTGGAAGACTGGTGTGAGGGTAAGAGAGAACA AGGATGGTTTGGTGGAGAGAGGAGAGATCAGGAGGTGTTTGGAAGCCGTGATGGAGGAGAAGTCGGTGGAGTTGAGGGAA AACGCAAAGAAATGGAAGCGTTTAGCGATGGAAGCGGGTAGAGAAGGAGGATCTTCGGATAAGAACATGGAGGCTTTTGT GGAGGATATTTGTGGAGAATCTCTTATTCAAAACTTGTGTGAAGCAGAGGAGGTAAAAGTAAAGTAAATAAAGGACAAAA GAGTTGGTGTCTAGAAGTGGCGGAGTGTTATTTGCTAATACTGTCACTTTGTAATATCAAATTCAATTAAAACATAAAAT TTACTC ...........................i...........................................N........ ..........N......N.........N...N...........................................N.... ...........N.................................................................... .................i.............................................................. ..............................................................................i. ..............................................................N................. ................................................................................ .............................................................N.................. ................................................................................ ....N.............i..i.......................................................... .....................................i.......................................... ................................................................................ ..................i............................................................. ................................................................................ ...................................N............................................ ................................................................................ ....i........................................................................... ...N..............................................i............................. ...........N...............i......................................i............. ................................................................................ ................................................................................ ...... Pos Score Pred ------------------------ 28 0.569 Yes 72 0.100 - 91 0.328 - 98 0.388 - 108 0.440 - 112 0.247 - 156 0.455 - 172 0.261 - 258 0.852 Yes 399 0.648 Yes 463 0.423 - 562 0.719 Yes 702 0.465 - 805 0.452 - 819 0.701 Yes 822 0.748 Yes 918 0.750 Yes 1059 0.564 Yes 1236 0.256 - 1365 0.762 Yes 1444 0.223 - 1491 0.520 Yes 1532 0.303 - 1548 0.645 Yes 1587 0.626 Yes

Explain the output -- Processing locus 325: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAATACAAAATTACATGCCTTCTTAGCATATTATTTTCACATTATTAAACATAATTTCTTGTAACCCTAAGGAAGGAAC CGCACATCCATAATGACTTGACTGACTTTGTGTGTATGTAGCTATAGCGGCATTTTGGATCATCACCGGACCAATCGACA TCACATGTTCCTTAGTATTGATTCCTGTTTTGAAGTTAAAAAAGATATTACGGTATGTGCTGGATATGCGTGGTCTAGTG TTGCAAAAAAAAAGGAAGAAGATTCAACGGCCACCAAATTTCATCATTTTAGCCAACAATGTATGTTAAATGCGATCAAT AAATTCATGCAGCTGCCATCTGTTTTCCTCTCTCTCTTCATTAACTTAATTTCTCTCTCTCACGCATTTTGTCTTTGCAA CATCACCACCATACATACATACAACTTACATACATACACGTCTTCCACAAGACTCAAACTCCACAGCATGGGATTCTCAC GGTCGCTCAACCGCACCGTGGGCGTCTTCGTCTTCTTCATCCTCGACATTGTTGATTTCCTGCTTTGCTTTACCTACAAG ACTCTTGATTTCTTCTTCGAGTCCGAGTGGAAACCCTGCTACTGCTGTCCGCCGCCCGAGGCCAAACCAATCTCGGCTGG AGGGAACAGAGGCGGTAAGATGATAGTGTCGGAAAGATCAGGAGACTACTCGAAGGTGGTCTCACTCACAAGGACAAAGA TCTATCTGGATGAGATCTCCGACACACTCTACTCTCGCCCTTCACTCCTCACCAAGCTCACTAAGCTGGTGAAGTGCTTC AAGAAAGATGTTGTTAAATGCTGTGATGAGTCCAAGAAGAGATCACCGTCAACTAAAAAGACATTACTCACTGTCAACTC CACGGTGGTCGAGAAGTTGCAAAGAACACCAAGATGGTCTGATTGCCATTGCACTTTCTGCACTTCTTGGCTTTCCTCTT CCAACCAATCTCTCTTTGTCAATGTCCAACAGCCCACAGACAACAAGGCACAAGAGAACGTAGTGTTCATTCATGGGTTC TTATCATCATCCACGTTTTGGACGGAGACTCTGTTCCCAAACTTCTCAGACTCAGCCAAATCGAATTACAGGTTTCTCGC AGTGGACCTGTTGGGTTATGGGAAAAGTCCAAAGCCGAACGATTCATTGTATACATTAAAAGAGCATTTAGAGATGATAG AGAGATCGGTGATTTCTCAGTTCAGACTCAAGACATTCCATTTAGTGGCTCACTCCCTAGGCTGCATTTTGGCTCTTGCG CTCGCGGTTAAACACCCAGGAGCCATCAAGTCACTCACTCTATTGGCTCCTCCATATTATTCGGTGCCAAAAGGTGTGCA AGGGACGCAATATGTGATGAGAAGACTGGCCCCCAAGGAAGTGTGGCCTCCGATGGCCTTCGGAGCCTCAGTGGCTAGCT GGTACGAGCACATTAGCCGCACAGTCAGTCTAGTGCTTTGCAAGAATCATCACTTGTTGGAATTTCTCACCAGGCTACTC ACCAGAAACAGGATGCGAACGTACTTGATAGAAGGGTTCTTGTGTCACACACATAACGCGTCATGGCACACACTACACAA CATCATTTTCGGATCGGGGAGCAAAGTAGAGGCTTATTTGGACCATGTCCGTGACAACGTGGACTGTGAGGTCGCAGTCT TCCACGGCGGTAGGGACGAGCTGATCCCCGTGGAGTGCAGCTACGGCGTCAAGAGGAAAGTGCCACGTGCAAGGATCCAC GTCGTCCCCGACAAAGACCACATCACCATTGTTGTTGGGAGGCAGAAGGAGTTCGCTCGTGAGCTTGAGCTCATTTGGCG CAGATCCACTACTCCTCAACTTCATTCAATAAATTAACCCTTCTTTATTTTAAGCTAAGTATATATATATTAATACCAAC CACTATTATTCAAACCATCATTTTATACCCATTCACTTTTGCATATTTCGGTGACT ...............N................................................................ ............N......................N............................................ ....i.................................................N..........N.............. ..........................................................N...N......N.......... ......N......................................................................... ...................................................................i............ ................................................................................ ................................................................................ ...................i............................................................ .........N...................................................................... .......i.........i.......N...................................................... .................................N.............................................. .....................N..................................................N....... ................................................................................ .................N.......................................................i...... ................................................................................ ................................................................................ ...........i....N...................................N........................... ................................................................................ ............N.................................................N................. ............................................i................................... ................................................................................ ................................................................................ ................................................................................ ........................................................ Pos Score Pred ------------------------ 16 0.458 - 93 0.398 - 116 0.187 - 165 0.543 Yes 215 0.402 - 226 0.183 - 299 0.475 - 303 0.218 - 310 0.170 - 327 0.418 - 468 0.654 Yes 660 0.532 Yes 730 0.427 - 808 0.593 Yes 818 0.800 Yes 826 0.450 - 914 0.433 - 982 0.415 - 1033 0.260 - 1138 0.266 - 1194 0.716 Yes 1372 0.552 Yes 1377 0.258 - 1413 0.358 - 1533 0.167 - 1583 0.293 - 1645 0.644 Yes

Explain the output -- Processing locus 326: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTTTCTATTTTCTCTCACAAAAGAGAAACCAAACAAGGTTTTGATTAATTTGTTTAGCTAAAAATAAATAAACTGCATT ATACACTTCAAATCGTGCTAATTAGGAAGCCCAAATCATATGGAGGACGAGCCACTTTTACCACTTATGGGCCTGTTATT AGTGGCCCAAGACGAGACGGATGTCGGATCATGCGATTTGGCTGTTGCAATCCATAGACACCACCGAGCGAGCATCTTCT TCTTCTTCTTCGCTAACCGGAGTAATCGTAAGCAGCTTCGACTGGTGAAAATGTGTTACTCTCTCTCTATACAATCTTCA ATTGACTTCCATAATCGAAACGCTCTAAAAATCCATGGAGATCGAGCAATTTTGACTTCGAACTTACCTACACTCAGGAG GATTCCGTTTTTGCCGGAGCGAGATCGCCGGAGAAAGTTGGTCCTCTGTACCGGGAGAGTCGTAAACTCATTGAAATTCA CCGGAAACACTTCCGTCGATCTATGTGGTATTCCTCGTCATCGATTAAGAGTTTCTTGCAGCGATGCTCGCCGTACACCG GAAGAAACGGCGGCGGAGTTGACGGCGCAACCCAATTTTTCAGAATTTATAACGTCGGAGAGAGTCAAAGTGGTTGCGAT GCTCGCGCTTGCTTTGGCGCTATGCAATGCGGATCGTGTTGTGATGTCCGTTGCGATTGTTCCTCTTTCGCTTTCTCGTG GATGGAGCAAATCATTTTCCGGTATCGTTCAGTCATCGTTTCTATGGGGATACTTAATTTCTCCAATTGCTGGTGGAACT TTGGTGGACCGTTATGGTGGTAAAGTAGTCATGGCGTGGGGTGTGGCTTTGTGGTCGTTGGCAACTTTTCTTACCCCTTG GGCGGCTGATAGTTCTTTGTGGGCTCTTCTTGCTGCAAGAGCGATGGTTGGGGTTGCTGAAGGAGTGGCTCTTCCTTGTA TGAACAACATGGTTGCGAGATGGTTTCCCCCAACAGAACGTTCTAGGGCGGTTGGTATTGCAATGGCAGGGTTTCAGCTT GGGAATGTTGTAGGACTCATGTTGTCTCCTATTCTTATGTCTCAAGGAGGTATATATGGCCCATTTGTAATTTTTGGACT ATCGGGGTTCCTTTGGTTGCTCGTGTGGCTCTCTGCAACGTCAAGTGCACCAGATCGACATCCTCAGATTACAAAGTCTG AACTTGAGTATATAAAGCAAAAGAAGCAAATATCTACTATGGAGAACAAACGAATTAGCACAAGTGGAATACCTCCTTTT GGACGCCTTCTCTCCAAGATGCCGACTTGGGCTGTTATAGTTGCAAATTCCATGCATAGCTGGGGTTTCTTTGTGATTCT TTCATGGATGCCCATATACTTCAATTCAGTATATCATGTGAACCTCAAACAAGCTGCTTGGTTTAGTGCCGTTCCATGGA GTATGATGGCTTTCACAGGATACATTGCTGGTTTTTGGTCGGATTTGTTGATACGGCGTGGTACAAGCATCACCTTAACG CGAAAAATCATGCAGTCCATTGGTTTCATTGGTCCTGGAATTGCTCTCATTGGTCTAACGACGGCAAAACAACCTTTAGT GGCATCAGCCTGGCTTAGTTTAGCCGTTGGGCTTAAATCATTCAGCCATTTGGGGTTTCTTATTAATCTTCAGGTACCCA AGCGTGTGATTCATACCTAACCCAATACAAATGATGAGTATCTTTAACATTTATTCCCACTTGTTTCAGGAAATTGCTCC CGAATATTCCGGTGTTCTACATGGTATGCTTTCTAATTC ................................................................................ .......................................i..........................N............. ....................i.........N................................................. ..................................................i............................. ..................................i............................................. ................................................................................ ......................N........................................N................ ..............................................................................N. .....................N....N................N.................................... .............N................i................................................. ...........................................i...................................N ........i..........i..........................................i................. ....N.............N.................N..................N........................ ................................................................................ ......................................i......................................... ..................N................................N............................ ...N...N...........................N.......................................i.... ..N..i.......................................................................... .........i...................................................................... ................................................................................ ..............................N..N.............................................. ....................N....N............. Pos Score Pred ------------------------ 120 0.731 Yes 147 0.197 - 181 0.737 Yes 191 0.478 - 291 0.664 Yes 355 0.552 Yes 503 0.202 - 544 0.426 - 639 0.360 - 662 0.208 - 667 0.304 - 684 0.277 - 722 0.224 - 764 0.244 - 814 0.135 - 831 0.860 Yes 924 0.701 Yes 960 0.228 - 969 0.543 Yes 980 0.603 Yes 1023 0.519 Yes 1045 0.156 - 1059 0.227 - 1077 0.173 - 1096 0.414 - 1239 0.790 Yes 1299 0.192 - 1332 0.239 - 1364 0.262 - 1368 0.301 - 1396 0.279 - 1436 0.540 Yes 1443 0.220 - 1446 0.738 Yes 1530 0.609 Yes 1711 0.427 - 1714 0.132 - 1781 0.036 - 1786 0.043 -

Explain the output -- Processing locus 327: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATCCCTCAATTCAAACTTTCTAAAAGGTAGCAATTAATGAGATTTAATTTTACAACTTTGCAACGAAGCTCGAAACTC TCTCTCTTCCTCTATATATAGAGTCATACTTTACCTAGAAAATATATGCACCAAACAACCATGGAAAAACATGATCTAAC TTTGATTTCCAAAAAACGGAGGATAGATTTTGCGACAACCGGAGATATCCCAAACCTTAAAGGCGAGGGTGTTTCTATGG TCGCTGAATCTGCTTCTGCGTCAAATATTTTTGCGGACAGTCTAGTCTATAGATTGTTCTTTAAGGGTTTGGTGAGTGAT GAAACAACTACCGACATGGAAGAGATTGTGAAGGCTGGATTTGGGATAGCAATTTGCGACGAGGCAAATACTTTGTTGTA TAATATGAAGAAATCACTGAACGGCGATGATGTGATTAATCCCGAAGAAGTAGAGATTAAAGCACTGATCTGTGTGTTAA ATGTCTCAATTCAAATGGAGCTTAGAAATGTCATGATTTGCTGTGGTGACTATCAAATATTCCAAATCCTTACTGGTAGA GGGAAGCCTCAGCAGAACATTGTCCATCTGGTGGAACAAGTCCAACACCTTAGAGGGAAGCTATCTTCTACTGAAGTTGT TTTGGTTCCGCGAGCTGATGTTATTATACTTGCAATAGAGGCAATAGGAGGAGAAACTTGTTGCATTTGTCGTGAAAACA CCGATGCTGATCGCATGTTCTTTACGGAGAATTGCTTTCACCGTCAATGCTTTTCTTGCGTGAATAGGCATGTGCAACGT ATGCTGCTTTGCGGAATTTCACCCACATGCCTTCATTTTCCTTGCAACTCTGAGCTCACGTTTGAAAGCTGTAGCAAGGT TTTGACACCAAATCTGATTGAGTTTTGGAAACGAAAGATTGAAGAGGACTTAGTTCCGGCTGCGGACAAAATCTATTGCC CATATCGAAGATGCTCAATGTTGATGTCCAAAACCGCGCTTTCCCGTGAAACCGACCAATCCAATGTCCGGGCATGTATC AAATGCTGTAGACTCTTCTGCATTGACTGCAAAGTACCATCGCATGCTGGTTTGTCGTGTGTTGATTACAAGAAACTTAA CCCTGATCCTCTATATGATGTCAAGCTCAAGTCTCTAGCAAACAAGAAGAAGTGGCGTCAATGTGTCCAGTGTAGCAACT TGGTTGAACTTTTTGAAGGTTGCAACCACATAACTTGCAGATGTGGATTTGAGTTTTGCTACGTATGTGGGAAAGAATGG AACCAACGAGGATGCCTTTCTCCTGGTTGCGATGATGATGATGATGATGATGATGATGATGACGATGATGATGACGATGA TGATGATGATGACGATGATGATGACGACGATGAGGATGATGAAGACGACGGCTATATTGACAGCGATGATGATGATGTTG ACGGAGATGACAATGATGATGGCTCAGAATTAGATGGGATTAACTTTGAAGATTTTGCGGTTCACCTACCCAAAGATGTC AATGAGCAGAAAGATGAGAGCAACTGAAGTGCTAGGAGAGGTATTAATGTGAAAGTCATGAAATGTTTTCTTTTTCAGTT TTTCTTTTGAAATTTAGATAATAAGATAAGTCATGTAATGCTAAAG ......................................N......................................... .............................................N..............i.........N......... ............................................................................i... ..............................................................................N. ...............i................................................................ ....N.....................N..N.................................................. N.............i............N....N............................................... ................................................................................ .................N.............................................................. ...N..........N...............................N......................i.......... N.........................N..................................................... ................................................................................ ..........N......i.....N.......................................i.........N...... ..N........................................N.................................... ..............N..N..........................................i................... ........................................N.......................N...........N... ...........N...................N..N..N..N..N..N..N..N..N..N.....N..N..N.....N..N ..N..N..N.....N..N..N........N.....N..N..........................N..N..N..N..... ......N.....N..N..N..............N.........................................N.... ................................N....N........ Pos Score Pred ------------------------ 39 0.448 - 126 0.353 - 141 0.571 Yes 151 0.255 - 237 0.748 Yes 319 0.168 - 336 0.509 Yes 405 0.423 - 427 0.099 - 430 0.220 - 481 0.123 - 495 0.562 Yes 508 0.421 - 513 0.337 - 658 0.071 - 724 0.414 - 735 0.280 - 767 0.221 - 790 0.648 Yes 801 0.464 - 827 0.241 - 971 0.173 - 978 0.534 Yes 984 0.193 - 1024 0.679 Yes 1034 0.238 - 1043 0.133 - 1084 0.366 - 1135 0.189 - 1138 0.264 - 1181 0.628 Yes 1241 0.096 - 1265 0.052 - 1277 0.064 - 1292 0.051 - 1312 0.193 - 1315 0.255 - 1318 0.215 - 1321 0.306 - 1324 0.225 - 1327 0.197 - 1330 0.196 - 1333 0.247 - 1336 0.086 - 1339 0.186 - 1345 0.153 - 1348 0.152 - 1351 0.173 - 1357 0.120 - 1360 0.101 - 1363 0.128 - 1366 0.130 - 1369 0.176 - 1375 0.079 - 1378 0.175 - 1381 0.217 - 1390 0.186 - 1396 0.142 - 1399 0.105 - 1426 0.095 - 1429 0.131 - 1432 0.118 - 1435 0.138 - 1447 0.160 - 1453 0.192 - 1456 0.110 - 1459 0.278 - 1474 0.110 - 1516 0.144 - 1522 0.146 - 1534 0.252 - 1567 0.063 - 1578 0.064 - 1583 0.103 - 1633 0.076 - 1638 0.043 -

Explain the output -- Processing locus 328: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAATCACGTGATTCTCGTTCCTCTTCCTCACGAAACCCATCATCTTCTATCTCATTTGTAAGCAGCGATCTCAATCCA AATACCTTTAATTTTATTTGATTCCATACCCTAATTTTTGTATATTCCATGTCAATGAACGTTTCAGGAGAAATGGGTCA AAAGTTTTGGGAGAATCAAGAAGATCGAGCGATGGTTGAATCCACCATAGGCTCTGAAGCTTGCGACTTTTTCATCTCAA CAGCTTCAGCTTCCAACACTGCCTTGTCCAAGCTTGTCTCACCACCAAGTGATTCCAATCTCCAACAAGGGTTACGTCAC GTTGTTGAAGGATCTGATTGGGATTATGCTCTTTTCTGGCTAGCGTCCAACGTTAATAGCTCTGATGGTTGTGTCTTGAT CTGGGGAGATGGTCATTGCCGTGTCAAAAAGGGTGCTTCAGGTGAGGATTACTCTCAGCAAGATGAGATCAAAAGACGTG TGCTTCGCAAGCTTCACTTGTCGTTCGTTGGTTCAGATGAAGATCATCGTTTGGTGAAATCAGGAGCTCTTACTGATCTC GACATGTTTTATCTGGCTTCTTTGTACTTTTCCTTTAGGTGTGATACCAATAAGTACGGTCCTGCTGGAACCTATGTGTC TGGGAAGCCTCTTTGGGCTGCAGATTTGCCTAGCTGCTTGAGTTATTATAGGGTTAGGTCTTTCTTAGCTAGGTCAGCTG GTTTTCAGACTGTGTTGTCTGTACCAGTGAATTCTGGAGTTGTGGAGCTTGGTTCTTTAAGACATATTCCAGAAGATAAG AGTGTGATTGAGATGGTGAAATCAGTGTTTGGTGGGTCTGACTTTGTTCAGGCTAAAGAAGCTCCTAAAATCTTTGGTCG ACAGCTGAGTCTTGGTGGAGCAAAACCTCGGTCTATGAGTATTAATTTCTCCCCGAAGACCGAGGATGACACGGGTTTCT CATTGGAATCGTATGAGGTGCAAGCGATCGGAGGCTCTAATCAAGTGTATGGTTATGAGCAAGGGAAAGATGAGACATTG TATCTAACTGACGAGCAAAAGCCGAGGAAGAGAGGGAGAAAACCAGCAAATGGAAGAGAAGAGGCTCTAAACCATGTGGA AGCGGAACGGCAGAGGAGGGAGAAGCTGAACCAGAGATTCTACGCTTTGAGAGCGGTGGTGCCTAACATCTCCAAGATGG ACAAGGCTTCGCTCCTTGCAGACGCAATCACTTACATCACGGATATGCAGAAGAAAATCAGGGTGTATGAAACAGAGAAG CAGATAATGAAGAGGAGGGAGAGTAATCAGATAACTCCAGCAGAGGTTGATTATCAACAGAGGCATGATGATGCAGTTGT AAGGCTAAGCTGTCCGTTGGAAACTCATCCAGTTTCAAAGGTGATACAAACGTTGAGGGAGAATGAAGTTATGCCTCATG ATTCCAACGTGGCCATCACAGAGGAGGGTGTGGTTCACACATTCACTCTCCGGCCTCAGGGTGGCTGCACCGCTGAGCAG TTGAAGGACAAGCTCCTTGCCTCTCTATCACAGTAACTATCACAGCAGTAACTGCTATGTAATAAGTGTAACCGTGTTGG AGGTTGTATCAATGTACTATTGCAAGCCAACCAAAAAAAACTCCAGCTTAGTAGG ................................................................................ ................................................N.....i.................i....... ...............................i................................................ ................................................................................ .........................N......................................N............... ........N.....................................................N................. ....................................N........................................... ...i.....................................................................N...... ................................................................................ ................................................................................ ............i................................................................... ..................................N..............................N.............. ............N...................................N.....N..............i.......... .................................................N.......................N...... ............................................................................N... ............................................i.....................N............. ......i.........................................................N..N..N......... ..............................................................i.......i......N.. ................................................................................ ........................................................N....................... ...........N........................................... Pos Score Pred ------------------------ 129 0.487 - 135 0.613 Yes 153 0.643 Yes 192 0.712 Yes 346 0.269 - 385 0.444 - 409 0.295 - 463 0.123 - 517 0.233 - 564 0.652 Yes 634 0.203 - 813 0.546 Yes 915 0.324 - 946 0.356 - 973 0.240 - 1009 0.217 - 1015 0.236 - 1030 0.558 Yes 1090 0.390 - 1114 0.325 - 1197 0.477 - 1245 0.515 Yes 1267 0.158 - 1287 0.755 Yes 1345 0.124 - 1348 0.151 - 1351 0.082 - 1423 0.503 Yes 1431 0.800 Yes 1438 0.321 - 1577 0.058 - 1612 0.061 -

Explain the output -- Processing locus 329: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCATGTCGTCAATCTCTATGGCTTCTTCCTGTTCTTCTAATTCCCCGCTTTCGTTTTCTTCTGAATCATATTTGATTTTT ATCTTCTCTCTCATTTTTTAAAATTATTTTTTCTTTATGGATCTTTGTAGCTTTAGCAAAAACCGACCAGCTGAGAAGAT GTCTTCATCGTACAACACAATTGCTTTGTCCTCCACTCCGACCTTCCTCCTCTCAAGTGCCGCTGCCGGACCCGGACCAA ACAACTTTAACCGTCAAGAGGCTGCGATGACCATGGTTCAACAACAACCCACTTCCTCCGTTGCTCCACCACCCAAGAAG CGAAGGAACCAACCCGGAAACCCAAATCCAGATGCTGAAGTGATAGCGTTATCGCCAAAGACGATAATGGCGACGAACAG GTTCCTATGTGAAGTGTGCAACAAAGGGTTCCAGAGGGAACAGAATCTGCAGCTCCACAGGAGGGGACACAACCTTCCAT GGAAGCTGAAACAAAAGTCCAACAAAGAGGTGAGGAGGAAGGTGTACCTTTGTCCGGAGCCCTCGTGCGTCCACCATGAC CCCGCACGTGCTCTCGGAGACCTCACCGGAATCAAGAAGCATTACTATCGGAAGCACGGTGAGAAAAAGTGGAAGTGCGA TAAGTGCTCTAAGCGTTACGCTGTTCAATCTGATTGGAAAGCTCACTCTAAGACCTGTGGTACCAAAGAGTATCGATGTG ACTGTGGTACCATCTTCTCTAGGAGAGACAGTTACATCACCCACAGGGCATTCTGTGATGCACTCATACAGGAGTCAGCA AGAAACCCCACCGTGAGCTTCACGGCAATGGCAGCTGGTGGTGGTGGTGGCGCCAGACATGGCTTTTACGGCGGAGCTTC TTCCGCTCTCTCTCACAACCATTTCGGTAACAATCCAAATTCCGGTTTCACCCCTCTAGCTGCTGCAGGCTACAATCTGA ACCGTTCATCTTCCGACAAGTTCGAAGACTTCGTTCCTCAGGCCACCAACCCTAATCCCGGTCCGACCAACTTCCTCATG CAATGCTCTCCGAACCAAGGACTGTTAGCCCAGAACAATCAGAGTCTCATGAACCATCATGGTCTGATCAGCCTCGGTGA TAACAACAACAACAACCACAACTTTTTCAACCTCGCATACTTTCAAGACACCAAGAATTCTGATCAGACAGGTGTTCCTT CTCTGTTCACCAATGGTGCTGATAACAACGGCCCATCTGCTTTGCTTAGAGGGCTAACTTCTTCATCTTCGTCAAGTGTG GTCGTTAATGACTTTGGAGATTGTGATCATGGAAACCTTCAAGGACTCATGAACTCTCTAGCTGCGACAACAGATCAACA AGGCCGGTCCCCTAGCCTTTTCGACCTTCATTTCGCAAACAATCTTAGCATGGGTGGCTCGGATAGGTTGACTTTGGACT TTCTTGGTGTTAATGGAGGAATTGTGAGCACCGTCAATGGCCGTGGTGGTCGTAGTGGTGGACCTCCTCTGGATGCTGAG ATGAAGTTTTCACACCCAAATCATCCATATGGCAAAGCTTGAGATGATCGTTATCTATCTACAAATCATCCAAATCATCA TCATCTAGATATGCTATTAAGTTCCTTGGAGATTTTATTCTAGATAAAGCTAGATATCCAA ..N..............N.............................................................. ....................................N.........................................i. ................................................................................ ..........................i.....i............................................... ...............................N..................................i............. ......i.......................................................................N. ...........................................................................i.... ................................................................................ ...........................................................................N.... .........................................................i...................... ...........................i..............................i..................... ................................................................................ .............................................................................i.. ..i.............................................i.........N..................... ................................................................................ ............N................................................................... .......i....................N...................i............................... .................................................i.............................. ............N.......................N...................................N....... N...........................N..............N.................................... ..........N.................................................. Pos Score Pred ------------------------ 3 0.146 - 18 0.328 - 117 0.358 - 159 0.801 Yes 267 0.732 Yes 273 0.766 Yes 352 0.322 - 387 0.561 Yes 407 0.573 Yes 479 0.418 - 556 0.689 Yes 716 0.479 - 778 0.552 Yes 828 0.808 Yes 859 0.579 Yes 1038 0.698 Yes 1043 0.730 Yes 1089 0.622 Yes 1099 0.427 - 1213 0.463 - 1288 0.553 Yes 1309 0.383 - 1329 0.592 Yes 1410 0.810 Yes 1453 0.393 - 1477 0.434 - 1513 0.096 - 1521 0.379 - 1549 0.246 - 1564 0.251 - 1611 0.057 -

Explain the output -- Processing locus 330: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTTTTATGCTTAAACTTTATGTCTTTGTTATTGCAGAGTGGTTAGTGTAAAGGTGGATGAAGAAACTAAGATTTGGTT TAGTCCCTGAATGCAGTATAGAGTCATTGCTTTGAGGTTGCTGGTAATTATTCTACCACTATGGTGAGGCAACAAAGAGC CTCTAAGGTTCATGAAGATAGACATCCCTTGTGTCAAGGTAAAGATGAGCTGGTGAAGCATGATATGTTGGATCCGCCAA GGTATCTTCGAAGAACGAAAGAAGATTTCCGCGGAAAGGCGCTGAGTTTTGGGGTTTTGGATTGGAAACAGTTTGAGAAA TGGAAGGATACTAATGCAGATGGAACCAAAGAAGCGTGTTGTAGTTACGCGGAAACAGCTGCAAGTTCGTTGGAGCTAGA TTTATCGACCGGTGTTGTCAAAAGATTGGAGGTAGATTCGAAGTCTCAACAAATGAGGAATCAGAGTTTCTCAGTTAGAT CACAAGCATTTTCAGATGCAACGGATGCTGATACAGTGATGAGAGTGGATCAAAAGGGAAAACGAGAGCATCAACTTCTG AGTATGAAGCAGAAAGAACTAAGTCATGCCTCTGAGCTTAGCTCGTGTATCTCTCCAGGATCAGAGAAATTTAGAACAGT TGAATGTCAAGACAGAAGGCATGATGTAGAAGGCGAGTGTTCAAGTCCGGTGAGTGTGATGGAAAGAAACCAAGAGAAAC CATGCTTGTTGGATCAAAACATTCCCACAATGTCATCCAAAAAGGAAAGAGATCCTTCACCTAACAGGCGGTTTAGCTTC AGTTTTAGTCAGATGAGTAGAAGTTTCAGTTCCAAAGAAAGCTCATCGTCATTGTCAAGCACATCTCATGCTTCAGCTAA ATCAGGTCCTTTGACATTCACCAACTCAGTTTACACCACTCACTCGACCAGAACAAAGTCTAATGGTCACAACAGAACTA GATCAGGTCCTATATTGAAACCCAAGACCGAAAAAAATAATGTACCATCATTACAAGTGGCTTCAAAACCTTCCAACACA AGACCACCAACCAAGGAAAAGAAGCAAAGCAGCTCAAGAGTTCACGCTCTTTTACAATTCACATTGAGAAAAGGTATCAA TCTGTTTCAGTTCGTTGTTGGAGACAACAGCAATAATGTTCTCGCAGCTACCATGAAGAGTTCAGATTCTTCTACACGGT CTTACACATTGTACACCGTCAACGAAGTAAAGAACAAGACCGGAAACTGGTTAAGCCGCCACAAGAACGAACATCCATTT GTACATACAATCATCGGCGAAATGAAGACTGTTACTACTTTTACTTCAGATTCATCAATCCACAAATCAGAAACTGTTTT GTTCGGTGTTGACTCGACTAATGAAGAGCTTGCAGCAATAGTTCAGACAAGAAACACAACTACAATAATACTCCCAAGTG GAGTTCACACATTGCCTAAAGACGGTAACAATTCTCCTCTCCCGTTGATTAACCGGTGGAAAACCGGTGGAGAATGTGAC TGCGGTGGTTGGGACATCGGTTGCAAACTCCGTGTCCTGTCTTATAATCATACAAAGACTCAAACTTTGTCAAGCTTTCA ATTGTTTGATCAGGAAAGAGACGAGCCAGCTTTCAAAATGGTGAGCCACGGCGATGAACTCCACTCTGTTGAGTTTGGCT CATCGATCTCTCTCTTGGAAGCGTTCTTTATTTCGTTAGCAGTAACTAGTCATCAGAGTTGGTGTCAGGAGGAAGAAGAA GAAGAAGTGGTTGTGATCGGAGATTGTCTGTTAAAGAGAGAAACACCGGCGAAGTACGCAACGAACCCACCGGTTTCTCC AATCGGTCGGGTCTAGAGGCAGAGAGAGTTGGGCTTAGACAGTGTTGGATCTGGTTTGGGCTGGGCTTTAATATAATTGT AATTAAGAATTACATGGCATAATTAATCGTTTAGA .......N............N.....................................N..................... ..........N.................................................i................... ...........N................................N..............N....i............... ...............................................................................N .............N.....N............................................................ ....................................................i........................... ...............N........N.............N......................................... ...N.....................i...................................................... ...i................N..N..................................N..................... ............N......................................................N............ ..............................................................i................. .......................................N........................................ ................................................................................ ...................................N................i........................... ................................................................................ .....................i.......................................................... ....................N........................................................... .........................................................................N...... ................................................................................ .....................................i...............N.......................... ................................................................................ ................................................................................ ................................................................................ .............N..................... Pos Score Pred ------------------------ 8 0.163 - 21 0.386 - 59 0.254 - 91 0.187 - 141 0.767 Yes 172 0.253 - 205 0.332 - 220 0.227 - 225 0.518 Yes 320 0.498 - 334 0.289 - 340 0.314 - 453 0.702 Yes 496 0.194 - 505 0.155 - 519 0.247 - 564 0.456 - 586 0.665 Yes 644 0.507 Yes 661 0.364 - 664 0.343 - 699 0.291 - 722 0.580 Yes 750 0.474 - 813 0.318 - 868 0.347 - 943 0.522 Yes 1000 0.478 - 1156 0.414 - 1173 0.736 Yes 1302 0.642 Yes 1381 0.204 - 1514 0.189 - 1638 0.872 Yes 1654 0.290 - 1934 0.050 -

Explain the output -- Processing locus 331: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTAAGTAAGTCACTATGCTTTAGTCCTCTACACCAAAACATGATCAAGAGGTATCTCATCCGAATATTCCACGTGTCAA GCAAGCGGTGGCTAGGATTGATTACACAGTCCACGTCAGCTCTCTAGAAACGGCTCAATGCTCTTGTCCTTGACACTTTT CTTCCACTTCTTCTTCATCATCTTTAATCGTTGGGAAAAGTTTGATGATACGGAGTGGACAATTCTTGGTGTAGAAGGAG AATCTGAGAGAGAGAGAGTCATGGAAGCTCTGAAAACCGCAACTTTTAGCCCTATGTCGGTGTTATCCGAGAAAAGATCA GAACCACGAAAGCCTTTCTCGCTCCCTAACCTCTTTCCACCAAAATCACAGAGACCAATCTCCCAAGAAAGCTTCTTGAA GAGATTCAATGGTGGATTGGCTCTTCTAACTTCTGTTCTAAGCAGTGCAACAGCTCCTGCTAAATCCCTGACGTACGAGG AAGCTCTGCAACAATCTATGACCACTTCTTCATCTTTTGATTCGGATGGTCTGATTGAAGGGATATCCAATTTTGTCACA GACAATCCTTTGGTTATTGCCGGTGGAGTTGCTGCATTGGCTGTTCCATTTGTTCTGTCTCAGGTTCTGAACAAGAAGCC CAAATCATGGGGAGTTGAGTCTGCTAAGAATGCTTATACCAAGTTGGGTACTGATGATAATGCTCAGTTGCTTGACATAA GAGCTACTGCTGATTTCAGACAAGTGGGCAGTCCTAATATTAAGGGTTTGGGTAAAAAAGCGGTTTCTACTGTTTATAAC GGTGAAGACAAGCCTGGTTTCCTGAAGAAGCTTTCTTTGAAGTTTAAAGATCCTGAGAACACCACATTATACATTCTGGA CAAGTTTGATGGAAACTCTGAGCTTGTTGCTGAATTGGTGGCTCTTAATGGATTCAAATCTGCTTATGCGATTAAAGATG GTGCAGAGGGACCTAGAGGCTGGTTGAATAGCAGCTTGCCTTGGATAGAGCCAAAGAAGACTCTCAGCCTTGATTTAAGC AGTTTGACGGATAGCATCAGCGGTGTATTTGGTGAGAGTTCTGATGGTGTATCTGTCGCTCTTGGAGTAGCTGCTGCTGC TGGATTAAGTGTTTTTGCATTTACAGAGATTGAAACCATACTCCAACTACTAGGTTCAGCTGCACTTGTTCAACTTGCAG GCAAGAAACTTCTATTTGCTGAGGACCGAAAGCAAACTCTAAAACAGGTGGATGAGTTCTTGAACACAAAGGTTGCCCCT AAAGAACTTGTTGATGAGTTAAAGGAAATAGGAAAGGCTCTTCTTCCTCAATCAACAAGCAACAAAGCTCTCCCAGCACC AGCAACAGTAACAGCAGAAGCAGAATCAGCTACAGCTACAACCACCACAGTCGATAAACCAGTACCTGAGCCAGAAACAG TTGCAGCTACAACAACCACTGTAGATAAACCAGTACCTGAGCCAGAGCCAGTGCCAGAGCCAGTGCCAGTGCCAGCGATT GAAGCTGCAGTCGCTGCACAAGTAATCACAGAACCAACCGAAACAGAAGCCAAACCAAAACCTCATTCAAGACCTCTCTC TCCATATGCATCGTACCCTGACTTGAAGCCTCCATCTTCTCCGATGCCATCGCAGCCCTGAAAACAGCTAAGAACAGTCT CTCTCTCGTTACTTAGTCTGGTTTTGTCCTCCTGCAAACAGAAGAACAAAAGTGGTCGAATATTGTAACTTGTTCTTTTT ...............i........................N....................................... .........................................................N...................... ............................................N................................... ....................N................................N.......................... ................................................................................ ........i....................................................................... .................i...........................N.................................. ................................................................................ ......i......................N.......................N.....N.................... ................................................................................ ................................................................................ ........N......................................N.................N...........N.. ................................................................................ ...........................................N.................................... ................................................................................ ...................................................N............................ .............N.................................................................. ................................................................................ ................................................................................ ................................................................................ .....N.....................................N.................................... ................................................................................ Pos Score Pred ------------------------ 16 0.586 Yes 41 0.385 - 138 0.428 - 205 0.211 - 261 0.393 - 294 0.164 - 409 0.543 Yes 498 0.579 Yes 526 0.105 - 647 0.526 Yes 670 0.259 - 694 0.189 - 700 0.091 - 889 0.232 - 928 0.311 - 946 0.130 - 958 0.342 - 1084 0.179 - 1252 0.338 - 1294 0.194 - 1606 0.142 - 1644 0.398 -

Explain the output -- Processing locus 332: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAGGTAACGGAGCTCAGGTATAGAATCTGTGTGTCGACTTGATTAGGGCAGATGTAGAAAAACCTGAGAAGTAGTCTT TTTCAGATTAACTAGGGTTAGGGTTCTGATTTGATGCTCGGTTTTAAGCAGGAGCAGAAGCTTCAATCTTTGATAATGTC GAGGAACAAGGGTTTGGCTGAGCAAGATCTTAAAAAATTGGATGTAACTGTGCTACATCCTCTGTCTCCTGAGGTCATTT CTCGCCAGGCCACTATTAATATAGGAACCATTGGTCATGTCGCTCATGGAAAGTCCACTGTTGTTAAAGCTATTTCTGGT GTCCAGACTGTCCGTTTTAAAAATGAATTGGAGCGTAACATTACCATTAAGCTTGGATATGCAAACGCCAAGATTTACAA ATGTGAGGATGAGAAATGCCCTAGACCAATGTGCTACAAGGCCTACGGAAGTGGAAAGGAAGACACTCCCAATTGTGATG TCCCCGGATTTGAAAACTCCAAGATGAAACTATTGAGGCATGTCTCATTCGTTGATTGCCCGGGTCACGATATTCTCATG GCAACAATGCTTAATGGAGCAGCCATCATGGATGGTGCACTACTTTTAATCGCTGCAAATGAAACTTGTCCACAACCACA AACGTCTGAACATCTTGCTGCCGTTGAGATTATGCAACTTAAGCATATCATCATCCTTCAGAACAAGATTGATCTTATTC AAGAGAATGTTGCCATTAATCAGCACGAGGCAATTCAGAAATTTATAATGAACACTGTTGCTGATGCTGCTCCGATCGTC CCTGTCTCAGCACAACTGAAATACAACATTGATGTGGTGTGCGAGTACATTGTCAAGAAGATCCCAATCCCTGAGAGGAA TTTTGTGTCACCCCCAAATATGATAGTGATTCGGTCTTTTGATGTCAACAAACCTGGTTATGAGGTTGATGAGATCAAAG GTGGAGTTGCAGGTGGAAGTATCCTACGGGGTGTTTTGAGAGTCAACCAATTAATCGAAATCCGACCTGGTATCGTTACC AAAGACGAGCGTGGCAACTCAAAATGCACTCCAATTTACTCTCGCATCATTTCACTCTACGCGGAACAGAACGAGCTTCA GTTTGCTGTTCCTGGAGGTCTAATAGGAGTTGGAACAACAATGGACCCAACTCTCACTCGTGCAGATCGATTAGTTGGTC AAGTCCTTGGTGAAATCGGTTCACTTCCTGATGTATTTGTTGAACTCGAGGTGAACTTCTTTCTTCTACGACGTTTGTTG GGAGTGAGAACAAAGGGATCAGAGAAACAAGGGAAAGTGTCAAAGCTAACGAAAGGAGAGATTCTGATGCTCAACATTGG TTCGATGTCCACTGGAGCCAAAGTTGTAGGAGTTAAAGTCGATCTGGCTAAACTGCAACTGACTGCGCCTGTTTGTACCA GCAAAGGAGAGAAAGTGGCTCTAAGCAGACGTGTGGAAAAGCATTGGCGTTTGATTGGTTGGGGTCAGATTCAAGCTGGA ACCACCATCGAAGTTCCTCCTTCACCTTTCTAAGCTATGTGTTATTTTTGTCCTTTTTGTTTGTGTTTCTGAAAAAAAGA TTGTTAAACCCCTCAAAAAGATAATAAAACTCTTTTTCTCGTTGGGGGATTT .....................................................N.......................... .................................N.........................................i.... .........................................N...................................... ....................................N........N.................................. ......................i...................................i..................... N.......i......i............N................................................N.. .......................N...............N.....................................i.. ......i......N.............i...N..........................N..................... ...............................N................................................ ......N........................................i...............N................ ...............................N................................................ ...................i.....................N.................N........N........... ................................................................................ .......................i........................................................ ........................................i....................................... ..............................N................................................. ..................................................................i............. ....N........................................................................... ................................................................................ ....................................N........................................... .................................................... Pos Score Pred ------------------------ 54 0.452 - 114 0.279 - 156 0.835 Yes 202 0.071 - 277 0.226 - 286 0.114 - 343 0.533 Yes 379 0.506 Yes 401 0.348 - 409 0.689 Yes 416 0.649 Yes 429 0.496 - 478 0.111 - 504 0.342 - 520 0.187 - 558 0.806 Yes 567 0.852 Yes 574 0.325 - 588 0.552 Yes 592 0.197 - 619 0.126 - 672 0.380 - 727 0.207 - 768 0.839 Yes 784 0.120 - 832 0.209 - 900 0.636 Yes 922 0.403 - 940 0.352 - 949 0.144 - 1064 0.857 Yes 1161 0.650 Yes 1231 0.217 - 1347 0.573 Yes 1365 0.404 - 1557 0.262 -

Explain the output -- Processing locus 333: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAACCCATCCATCTTGCATAAAAACCCTAGTTTCTGAACAGCCTTTCGAAACTTCTAGTGTTATTGTATTGGCTCTGATC AACGTTGTTCTAAAGTTGTGAACTTTGATTGATTGCAACAGAGTGTTTAAGATGAAATCTGTCGTAATGTCGAAGAACAA GGGCTTAGCTGAACAAGATCTAAGTAAACTTGATGTAGCTCAGCTGCATCCTCTCTCTCCTGAGGTCATCTCTCGTCAAG CCACCATTAACATAGGAACCATTGGCCATGTCGCTCACGGGAAATCGACAATCGTGAAAGCCGTTTCCGGTGTGCACACT GTCAAATTCAAGAGTGAATTGGAGCGTAACATTACCATTAAACTTGGTTACGCAAACGCAAAGATTTACAAATGTGAAAA ATGCCCTAGACCAGTAGCCTACAAGTCTTTCGGGAGTGGCAAAGAAGACAACCCAAGTTGTGATGTCTCTGGACATGAGA AGTACAAGATGAAACTACTAAGACATGTCTCATTTGTTGATTGCCCGGGACACGATATCCTAATGGCGACAATGCTGAAT GGAGCAGCGATTATGGATGGTGCTTTACTAATCATTGCTGCGAATGAGACATGTCCACAACCACAAACAGCTGAACATCT TGCTTCTGTTGATATGATGCATCTTAAAGATATCATAATCATTCAGAACAAGATTGATCTCATCCAAGAAAACGAGGCTA TTAAACAGCACGAGGACATCCAGAGATTTATAACGAACACGAATGCGGAAGGTGCTCCTATAGTTCCTGTCTCAGCGCAA CTGAAATACAACATCGATGTTTTGTGTGAGTACATTGTCAAGAAGATTCCACTCCCTGTGAGGGATTTTGTGTCACCTCC AAGAATGATTGTGATTCGTTCTTTTGATGTCAATAAACCTGGCTCTGATTATGATGGTATGAAAGGTGGAGTTGCAGGTG GGAGTATCCTTCAGGGTGTTTTGAAGGTGGGGCATATAATAGAGATAAGACCTGGAATCACTAGAAAAGATGAGAATGGA GACACTAAATGCACTCCCATTTACACGCGTATAACTTCATTGTACGCGGAACAGAACGAGCTTCAGTTTGCTGTCCCGGG AGGTTTGATTGGAATTGGGACAAGCATGGATCCTACGCTTACGCGTGGTGATAGGTTAGTTGGTCAAGTCCTTGGTGAAA TAGGTACTCTCCCTGATGTCTATGTTGAACTTGAAGTGAGTTTCCAGCTTCTGACACGTCTCATTGGAGTGAAGACAAAG GAAACAGAGAGGCAAATGAGAGTGTCAAAGCTAGTCAAGGGAGAGATACTGATGTTGAATATCGGATCGATGTCTACGGG AGCTATGGTTATTGGAGTAAAGAAAGATATGGTGAAAGTTAATCTGACACTCCCTGTGTGCACCAGCATTGGAGAGAAAG TGGCTATAAGTCGCCGTGTGGATAGGCATTGGCGTTTGATCGGTCGCGGTCAGATTGAGGCTGGAACCACCATTTTTATC CCTTCTCCTCCTTCTTTGGCATGATTATATTTGATGCATTCACATATACCAAACTTGTAGTCTCATTATATCTTCAGAGT ATTCCTAATCTGTCACCAAAGGAGATAACTAAACTTCTATGTG ................................................................................ ...................................................i..............i............. ................................N............................................... ...........................N.................................................... .......................................................................N........ i.............................................................N...........N..... ........i...............N.....................................i........i......N. ............i...N..........................N......i............................. .............i..i............................................................... ..........................................N..................................... ................N............................................................... ....i.....................N.......................N..N....i..................... .....................................................................N.....N.... ........i....................................................................... .........................i...................................................... ...............N.....N.......................................................... ...............i...................................i.................N.......... ....N.......................N................................................... ................................................................................ ....................N............N.............................................. ......................................N.... Pos Score Pred ------------------------ 132 0.536 Yes 147 0.782 Yes 193 0.306 - 268 0.204 - 392 0.421 - 401 0.839 Yes 463 0.265 - 475 0.227 - 489 0.519 Yes 505 0.177 - 543 0.837 Yes 552 0.721 Yes 559 0.264 - 573 0.506 Yes 577 0.198 - 604 0.104 - 611 0.564 Yes 654 0.698 Yes 657 0.599 Yes 763 0.245 - 817 0.172 - 885 0.730 Yes 907 0.200 - 931 0.155 - 934 0.149 - 939 0.606 Yes 1030 0.294 - 1036 0.314 - 1049 0.813 Yes 1146 0.536 Yes 1216 0.108 - 1222 0.169 - 1296 0.642 Yes 1332 0.554 Yes 1350 0.410 - 1365 0.345 - 1389 0.402 - 1541 0.426 - 1554 0.167 - 1639 0.053 -

Explain the output -- Processing locus 334: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAAATTGAACTGAGCCAAAATAAACGAATTCGAATCATAATTGATCAATCTAAACCGAATATAAACCGAATAAAACGA ACTGTTTATGCGCTCTAGTGACCAAGACCAATGATGAAATGGATGGGTGGTGATGTTGCATCCACGAGTCCACAAGTCTG TGAAAACATTGCACTCCAAAAATGGCTAACCAGCTCTTCTCTCCACCAAACCATGGCTTCTTTCAACCACTCTTGGCTCT CTTCTCCACTCACCGAAACTCCCGCTTTCTTTTTTTCTCCATCTCAACACCCAAAATCCTTGAAACTCTCACTTTTCCGA ACTCGGAGCAACTCGTCCTCACCGGACCGCTCGTCAGAGGTTGAGCTCGATGTGGATCCGGTGAAGCTCGCATTGAAGAA AGCCGAAGCTTATAAGAAATCGAAATCAGAGCAAAAAAAACCAGAGAAAAATGCCGGCGACGAGGAATTGCCGCTCTCTG TTAAGGCTGCTATGCAAAAAGCCAATGATTATAAGAAAAGGAAAGGACTTGGAACTGATGCTGTCGCAAAAGCTAAACCC AGTAATACAGATCAAAGTTTCGTTAGATTAGCAAATAAGGTTGTTGAAGATAATGATGTTAAGAAGAAAGAATTGAAAGT CTCCAGCATTGATTTCATGGGGCTTGGCTTTGCTGATAAGAAGAGCACGAGGGGGCTTCCAGCGGGACTTGTTCCTGTTG TTGACTATCTTCCTGAAGGAGACTTACCTGAGGTGGAGTTTATTGTTGGTGATAAAACAAGATTTGCGGAGAAAGTAAAG GAGGTTGAGCAAGAAGGAGATGGAAACTCTGATGTGTATAAACCCAAAGTTTCTACATGGGGAGTTTTCCCAAGACCTAG CAACATCTCTAAGACGTTTGGTGGTGGAAGGACACTCCGACCGGGGGATTCAGTTGAGACCGCTGAAGAGAGGATTGTGA GAGAAGAAAAGACAAAAAAATTGCTCATAGCCTACAAGGAATCACTCGGACTGAACATTGATCCCAAACTCAAACTTGAG TGTGAGAAGGCAATAGACGAGGGGAACTCGCTTATGGATTCTGGGAAACTCAAGGAAGCTTTGCCATACTATGAAAAGGT CATGGAGAAAATAGTTTTCAAGAGTGAGCTTCATGGACTAGCGGCTTTGCAGTGGTCTATTTGTCAGGATTCACTTCGAA AGACAGACAAAGCAAGGCGCATGTATGAGAAGCTTATTTCCCATCCAAACCCGGGAGTGAGCAAGAAAGCGAGGCAGTTC ATGTTCAGTTTTCAGGCAATGGAGATGCTGAAGGTTAAGGGGTCGAGCTTTGCTGAAGGAAACACTGGTTATCAGGACTA CTTTGAAGCCTTTGTTGAAGATAAAACGAATTACAAAGCGCAGGAGGAAAAAGAAGGAGAGGAAATGGGGATAAATGAGA CACTCCTATATGTGATTCTTCTTGCTTCTCCGATTCTAATGGTCTTCATTGTAGCTGCACAGAGAGGGTATACACACTGA AGACACAAATTCTTGTCAATTTCTTACATGTAAGTGTTGTATATATTATGAATTAATTCATTTTATTACAGTTAAGGATT AATAACACAAAGCTTCTTT ................................................................................ .......N......................N..i....i...N.........N........................... .....................i..............................N........................... ................................................................................ .................................................N.............................. ..................................................i............................. ...........N............N................................N...................... ....................................................N..N........................ ................i............................................................... ................................................................................ ...................N...........N........................N....................... ................................................................................ ................................................................................ .................................i....................................N......... ....................N...N....................................................... N.................i.....i....................................................... ................................................................i.........N..... .........N............................N......................................... ...........................N...................N................................ ................... Pos Score Pred ------------------------ 88 0.118 - 111 0.406 - 114 0.566 Yes 119 0.629 Yes 123 0.452 - 133 0.241 - 182 0.682 Yes 213 0.465 - 370 0.186 - 451 0.655 Yes 492 0.480 - 505 0.143 - 538 0.134 - 613 0.262 - 616 0.281 - 657 0.719 Yes 820 0.284 - 832 0.261 - 857 0.377 - 1074 0.545 Yes 1111 0.223 - 1122 0.462 - 1153 0.268 - 1221 0.376 - 1225 0.321 - 1281 0.274 - 1299 0.840 Yes 1305 0.728 Yes 1425 0.674 Yes 1435 0.143 - 1450 0.092 - 1479 0.131 - 1548 0.042 - 1568 0.045 -

Explain the output -- Processing locus 335: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATTCAAGATTTGGCTTTAGGAGCGTTATTCTCTGTTTAGTTAATTTGAGCTAAGATTCATATTTTCCTCTGTTTTTTT TCCATATTCTCTGAAGCATGACAAAAGATTCACACTTTCCTCTGTTTTCTTATGTCAAAATTAGAACCAATAACTGAAAG ATTTGGTCAAGAAGAGGAAATGGGAAGAACATGTTTGCCGAGATGGCGATTAGGAAGACTAATGGCAGGATTACAAGTCA TCTTAGGATTACTGGTGATCACTGTGAGCCTCTCATGTCTCTACCGATTCCATTCCGTGGGTTACTTCTTGCAAAACGAA GACACTTGCAACAATATCTACACAATCAAAGAAGTCAGCAGCGAAGGATTCGACCTAAAGGCGCTGCACGATCGTGTTGA TGAAGTACTAGAAAAGATGGACAACTTGTACGAGAAGCTAGAGAAGACGGTAAAAGATATGGAGAAGAGCAAAGACGGAA GCAAAAAGGAGATGAAGAAGTTTCTTGAAGATGAAGTGATGAAGCCATTTTATTATGCTCATATCGGATTAAGGCAAATC CGGTTGCCAAAACCCGAAGGAATCAGAAACTCAACGGAAAAGGAAGAGCCTTTGATCAATAAGTTTCTCATTGAAGAGAT AAGGCAGTACATTACACCAAAGGAGAATCGTGTTGGGAAGATAAACATGTTTGGAACAGAGAGAGTTTACAACACTATTG GTCATGCTTGTGCGTTGATGAAGATGGAGCTAGAGAAGTATATGGATTATGACGTTGGAGCGTATTGCGATGATGATTGG AACTTAGCACAGAAGCTGATGCTTAACGGGTGTGATCCGTTACCTAGAAGACGATGCTTGACAAGAGCATCAATGACTTA CCAAAAACCTTATCCGATCAATGAGTCACTGTGGAAGCTTCCTGATGATAGAAATGTAAGATGGGGAAATTATCAATGCA GGAACTTCGCGTGCTTATCAAGCAAGAACCCGAAAAGGGGTTACACGAAATGCAGCGGATGTTTTGAAATGGAGAAAGAG AAAGATAAATGGGTCAAGAACAGTAGTTTGTTAGTTGATTTTATGATTGAAGATGTTCTGAGAGTGAAGCCAAGTGAGAT CAGGATTGGATTGGATTATGGTGTTGGGACAGGAACATTTGCAGCAAGAATGAGAGAGAAGAATGTGACTATTGTCACAA CAGCTTTGAATTTGGGAGCTCCTTTCAATGAGATGATTGCTTTGAGAGGTTTGATCCCATTGTACATATCTTTGAACCAG AGGCTACCGTTCTTCGATAATACAATGGATATGATTCACACTACCGGGTTAATGGATGGTTGGATCGATCTTCTTCTGAT GGATTTCGTGTTATATGATTGGGACCGAGTGCTGAGACCGGGAGGGTTACTGTGGATTGATAGATTCTTCTGTAAAAAGA AGGATCTTGATGATTACATGTACATGTTCCTGCAGTTTAGGTACAAGAAACACAAGTGGGCTATTTCACCTAAATCCAAG GATGAGGTTTATCTCTCTGCATTGCTTGAGAAACCTCCTCGTGCAATTTAAGGATTACAATATGAAGTTTAAGCTTCAAT GTTTGTAATTGACATTCTTACTCATTTGGTTGTCTAATCCTTGTTCTAGTTTGAATACAACAGAGGTTTT ................................................................................ .................N.................................N............................ ...................N..........i...........i..................i.................. ..................................N............................................. ...............................................................................N ................N.........................................i..................... ...........N..................N.......N...............N......................... ................................................................................ ..............................................i................................. ...N.............N.....N.................N......i....................N..N....... ..................N..................................N..................i....... ....................N.......................N........i......i..............N.... .................................................N........N.........i........... ........i.................................N.........N........................... .................N...............................N............N................. ...........................N....N............................................... ........................i.....N....................N...N......................N. ..............N................................................................. .........N.......N.....N........................................................ ...................................................................... Pos Score Pred ------------------------ 98 0.497 - 132 0.209 - 180 0.268 - 191 0.521 Yes 203 0.740 Yes 222 0.522 Yes 275 0.350 - 400 0.177 - 417 0.477 - 459 0.638 Yes 492 0.354 - 511 0.384 - 519 0.137 - 535 0.121 - 687 0.695 Yes 724 0.202 - 738 0.499 - 744 0.483 - 762 0.324 - 769 0.560 Yes 790 0.168 - 793 0.217 - 819 0.357 - 854 0.396 - 873 0.609 Yes 901 0.492 - 925 0.487 - 934 0.647 Yes 941 0.643 Yes 956 0.469 - 1010 0.292 - 1019 0.221 - 1029 0.648 Yes 1049 0.500 - 1083 0.448 - 1093 0.198 - 1138 0.120 - 1170 0.394 - 1183 0.225 - 1228 0.084 - 1233 0.332 - 1305 0.701 Yes 1311 0.342 - 1332 0.391 - 1336 0.456 - 1359 0.137 - 1375 0.322 - 1450 0.196 - 1458 0.363 - 1464 0.133 - 1522 0.205 - 1582 0.091 - 1599 0.145 -

Explain the output -- Processing locus 336: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGTGAAGATTTTTAGGGTTCTGAGAAGTTTCCATCACTTGAAGTCTTTTAAATTTTAATAGTATATGATAATGGAATTG CCTCGATTCTAATGCAATTGACGACAATGCCCACAAGAACAGAGCAAAATGTCTCCATTCTCAGAAACCTCTGTTCTGCT ACTTCCCATGGTCGAGAAATGCATAAACCTCTTGCAAACCTACGGCGTTTCATCGATAACGAAACTCAGACAAATCCACG CTTTTTCAATCCGCCATGGAGTCTCAATCTCAGACGCCGAGCTTGGGAAACACCTCATCTTCTACCTTGTTTCTCTTCCT TCTCCACCTCCCATGTCTTACGCTCACAAAGTCTTCTCCAAGATTGAGAAACCCATCAATGTCTTTATTTGGAACACATT GATCAGAGGCTACGCCGAGATTGGTAATTCCATCTCTGCTTTTTCTCTATACCGTGAGATGAGAGTCTCTGGTTTGGTCG AGCCAGATACTCATACTTATCCGTTTCTTATTAAGGCTGTGACGACAATGGCGGATGTTAGATTAGGAGAGACGATTCAT TCGGTTGTTATAAGAAGTGGCTTTGGTTCGTTGATCTATGTTCAGAACTCTCTGCTTCATTTATACGCAAATTGTGGAGA TGTAGCTAGTGCGTACAAGGTGTTTGATAAAATGCCTGAGAAAGATTTAGTGGCTTGGAACTCTGTGATTAATGGGTTTG CGGAAAACGGTAAACCAGAAGAAGCTTTAGCGCTTTATACTGAGATGAATTCCAAGGGTATCAAGCCTGATGGGTTCACA ATTGTTAGCTTGTTGTCTGCTTGTGCAAAGATTGGTGCTTTGACATTGGGTAAGAGAGTTCATGTCTATATGATCAAAGT AGGTTTGACACGAAACTTGCATTCCAGCAATGTGCTCTTGGACTTGTATGCGAGATGTGGAAGAGTGGAAGAAGCCAAAA CACTTTTCGATGAAATGGTGGACAAGAACAGTGTCTCGTGGACTTCGTTGATAGTCGGTTTAGCTGTTAATGGTTTTGGG AAAGAAGCCATTGAGCTTTTCAAGTATATGGAATCAACGGAGGGATTGTTACCATGTGAGATCACGTTTGTTGGGATATT ATACGCTTGTAGCCATTGCGGGATGGTGAAGGAAGGTTTTGAGTATTTCAGGAGAATGAGAGAGGAGTATAAGATTGAAC CTAGGATCGAGCATTTTGGTTGTATGGTCGATTTGTTAGCTAGAGCTGGACAAGTGAAGAAAGCATATGAATACATCAAG AGTATGCCGATGCAGCCTAATGTTGTAATCTGGAGAACATTGTTAGGAGCTTGTACTGTTCATGGTGATTCAGATTTAGC AGAGTTTGCTAGAATCCAGATCTTACAACTAGAACCAAACCACAGCGGCGATTATGTTCTCTTGTCGAATATGTACGCAT CCGAGCAGCGTTGGTCCGATGTGCAAAAGATACGGAAACAAATGTTAAGAGACGGTGTGAAAAAAGTCCCCGGTCACAGC TTAGTAGAAGTAGGAAACAGAGTTCATGAGTTTCTTATGGGTGATAAGTCTCACCCACAAAGCGATGCTATTTACGCTAA GCTGAAAGAAATGACTGGTAGATTGAGATCAGAAGGTTACGTTCCACAAATTTCTAATGTCTATGTAGATGTTGAAGAGG AAGAGAAGGAGAATGCTGTGGTTTATCACAGTGAGAAGATTGCTATTGCATTTATGTTAATTAGTACACCAGAAAGATCA CCGATTACAGTCGTGAAGAATCTTAGAGTATGTGCAGATTGTCACTTAGCAATCAAACTTGTGTCAAAGGTTTATAACCG AGAGATTGTAGTCAGAGATCGTAGCAGGTTTCACCATTTTAAAAATGGTTCTTGTTCTTGCCAAGATTACTGGTAGTCTG GTAATGCAGAAAATAAAATTATTGTTTCATCGCAGATCGTTCAAATTCTACGGACAATCTTCGTTAATTAAAGTCTTCTA GGGGAAATTTGTAAA .................................................................N.....N........ ...........N..............N.....................i............................... .......N..........N............................................................. ...............N................................................................ ............i.............................................N..................... ..........................................................i..................... ...............................................i......N......................... .....................................N.........................................N ...............................i.......................................N........ ............................................N........................N.......... .............................................................N.......N.......... .............................N.................N......N......................... .........N....i......................................................N.......... ...........................N.........................i.......................... ......................N................................i........................ .......................N..........................................N............. ...i.....i.........N.........................................N.................. .....................................................N................i......... ..................N......................i...................................... .........................N..........N...........................N............... ..........i.............................................N.....N.....N........... ............N........................................N.......................... .............................N.................................................. ............................................N................................... ...N............................................................................ ............... Pos Score Pred ------------------------ 66 0.149 - 72 0.349 - 92 0.373 - 107 0.466 - 129 0.657 Yes 168 0.342 - 179 0.483 - 256 0.299 - 333 0.814 Yes 379 0.391 - 459 0.679 Yes 528 0.507 Yes 535 0.118 - 598 0.078 - 640 0.205 - 672 0.822 Yes 712 0.132 - 765 0.352 - 790 0.222 - 862 0.200 - 870 0.210 - 910 0.300 - 928 0.130 - 935 0.252 - 970 0.204 - 975 0.747 Yes 1030 0.332 - 1068 0.198 - 1094 0.559 Yes 1143 0.328 - 1176 0.721 Yes 1224 0.293 - 1267 0.185 - 1284 0.538 Yes 1290 0.567 Yes 1300 0.178 - 1342 0.192 - 1414 0.307 - 1431 0.526 Yes 1459 0.133 - 1482 0.569 Yes 1546 0.219 - 1557 0.217 - 1585 0.185 - 1611 0.825 Yes 1657 0.192 - 1663 0.116 - 1669 0.125 - 1693 0.262 - 1734 0.237 - 1790 0.274 - 1885 0.212 - 1924 0.092 -

Explain the output -- Processing locus 337: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTTGTTTTGAGTGGTTTTGGAAGATGTGTCGTGTTAGATGGGGATTTCGTTAGGGTTTCTGTGAGCTAAAAAAGGGTG TATGTTTCTTTCCCTAATTGATTTTCGTTTGTAAATTTATTTTGCAGAATGATAACTACATTTTCATGGATACCAAAAGG GGTTGCAAAGGCCATGCGTGCTGATGAACTTCCTTCTAAGGAGGAAATCGAAATGCACAAAGACAGTTGTAAATATTTCA GTAATGATACAGAAGAAGAAGAGACCGAGGATGAAGAAGAGAATGGTGAAGTTGCTCATGCGAAAGCCGTAGCAGAAGAG TTTGGAAAATCATCCAAAAGCAAGAATGTTTCTTCTTCAATGGAGCTTGATGATGAGGTAGCTGAGGGTTTGAAAGAACT CGACATGGATAATTACGATGAAGAGGATGATGGAATTGAAATTTTTAGCTCCGGGCGTGGGGATCTTTACTATAAGAGTA ACGAGATGGATCCATATCTCAAGAGAAATGATGATCATGATGATGACTCGGATGATGATCCACCAATCTTACCAACCGAT TTAGTGATTGTCTGCGCTATGACTGACGACAAAGAAGCTAACCGTCTTGACGTTTATGTATCCGAGGACACATCCCATGG TTCTCCAAATATGTATAATCATCATTACAGAATTATACCAGCAATTCCATTGTGTACTGCTTGGCTTGATTGTCCACTTA AAGGAGGAGAAAGAGGGAATTTCCTAGCTGTTGGTTCGGATGGGACGCCAACAATAGAGATATGGGATCTTGATGCTTGG TTCGACATGCTACCTTGTGTACAACTAGGAGGACAAAACAAGGAAGGGAACTATAAACAAGGTAGCCACACTAGATCAGT TCTTGGTCTTGCTTGGAATAAGGAGTTTCGGAATATACTTGCTAGTGCTAGTGCCGACAAAAAAGTTAAGGTATGGGATG TGGCTACTGGAACGTGTAAGATTACTATGGAGCATCACACAAAGGAGGTTCAAGCGGTTGCTTGGAACCATTATGCTCCA GAAGTGCTTCTCAGTGGGTCCTTTGATCAAACTGTTGTATTGAAAGACGGAAGACAACCTTCACACTCGGGTTTCAAATG GTCTGTCATGTCCGATGTTGAAAGCTTAGCCTGGGATCCTCATAGTGAACACTCCTTTGTGGTAAGTCTCGAAGATGGAA CTGTCAAGGGCTTTGATGTACGTCAAGCCTCCATTTCAGCCTCTGAATCAAATCCAAGCTTCACTATTAATGGACATGAC GAAGCAGCTACTTCCGTCTCATACAACATTTCGGCACCTAATCTTTTGGCAACGGGATCTAAGGATAGAACGGTAAAGCT TTGGGATCTTTCAAACAATGAGCCTTCATGCATTGCTACACACAATCCAAATGCTGGAGGCTTATTCTTCATTGCTTTCT CTCCGGACAATCCTTTCTTACTCGCTATGGGTGGCGTAATGGGGGAGTTAAAACTTTGGGATACACTTTCGGACACTAAT GTCTCCAGCAGATACGGAAGCAGAGAAGTCCGACCGTGAAAAGTCCAAGCTAAACTTTGATTGGTCGTCACATTCGAACA CAACTTAACCACAATTTTGTTTAGTTTCTTTCTTTCATTAATGTTCGTTTTATTTCTT .........................N.............N........................................ .............i.........N............................i........................... ...N..........................N...........N..............N...................... .........................N.............i.........N..N........................... ....i............N........N..N.................................................. .....i.....................N..N.....N..N..N........N..N......................... ..................i....................................N....................i... ..........i..................................................................... .......................................N.....................N..........N....... ......N......................................................................... ........................................................................i....N.. ..........................i.............................................N....... .............................................................................N.. .......i......N...........................................................i..... ...............i.....................................................i.....N.... ................................................................................ .................N.........N......................N............................. ..........................i...........N.......................................N. ................................................................................ ........................................N................. Pos Score Pred ------------------------ 26 0.371 - 40 0.222 - 82 0.222 - 129 0.565 Yes 146 0.177 - 174 0.719 Yes 184 0.269 - 213 0.817 Yes 244 0.219 - 271 0.223 - 283 0.177 - 298 0.106 - 346 0.131 - 360 0.878 Yes 370 0.107 - 373 0.125 - 405 0.703 Yes 418 0.236 - 427 0.176 - 430 0.242 - 486 0.719 Yes 508 0.213 - 511 0.431 - 517 0.115 - 520 0.156 - 523 0.159 - 532 0.195 - 535 0.174 - 579 0.645 Yes 616 0.202 - 637 0.574 Yes 651 0.585 Yes 760 0.418 - 782 0.481 - 793 0.175 - 807 0.208 - 953 0.523 Yes 958 0.244 - 987 0.878 Yes 1033 0.189 - 1118 0.178 - 1128 0.811 Yes 1135 0.157 - 1195 0.519 Yes 1216 0.526 Yes 1270 0.506 Yes 1276 0.280 - 1378 0.445 - 1388 0.331 - 1411 0.261 - 1467 0.782 Yes 1479 0.266 - 1519 0.147 - 1641 0.030 -

Explain the output -- Processing locus 338: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTAATTGGTTGTGAACATGATTTTGAAGTTTGGATATATAGCCAATGATAGTTGCTTCATGTTAGAGACTACATTACCA AAATTTCACATAATCTTATAAAGTTTTGTTTTTGATGCCAGGATTGTTATTGTTCCGCAACTGGAGGTGTTAAAACAGAT TTCTGATATTATCGGGCATCCTGTTCCAGGAGGAAAGCATAATGATCAACGTGGAGCAGATTCTGGTATTAAGTTTAATA CAACTGAGCAACAAGGTAGTGGTATCCGTCAAGTAAACAATATCGAGCCAGGGAGAAGTAATGCAGCTATTTCACCTCAG GTTGGTGGTACTGGTTCTAGTGTTCCTGCATCAACAACACCTTCCACTCGGGCATACAGCAATCCGTCTTCAGGTAATGG AGTCACTAGGCAGGACTATGCTCGTGATCCTCCAACAAGTTATCCTCATCAGCCTCAACCACCACCACCGATGTATGCAA ACAGGGGACCAGTGGCTAGGAATGAAGCACCTCCTAAGATAATTCCAGTTAATGCTCTTAGTCCATATTCAGGTAGGTGG ACCATCAAGGCCAGAGTCACGAATAAAGCAGCACTTAAGCAATACAGTAATCCCCGTGGGGAAGGGAAAGTGTTCAACTT TGATCTTCTTGATGCTGATGGCGGAGAAATACGTGTTACTTGTTTTAACGCTGTGGCTGATCAATTCTATGACCAGATTG TTGTTGGTAATCTTTATCTTATCTCGAGGGGAAGTTTGCGGCCTGCACAAAAGAACTTTAATCATCTTCGAAATGATTAC GAGATCATGCTGGATAATGCATCAACTATAAAACAATGCTATGAAGAGGATGCTGCAATTCCGCGCCATCAGTTCCATTT CCGTACCATTGGGGATATTGAGAGCATGGAGAATAACTGCATCGTTGATGTAATTGGTATTGTGTCATCTATCAGTCCTA CTGTAACAATAACGAGGAAAAACGGAACTGCAACACCGAAAAGATCACTTCAGTTGAAGGATATGTCAGGCCGAAGTGTT GAGGTAACTATGTGGGGTGACTTCTGCAATGCAGAAGGACAAAGGCTGCAAAGTCTTTGTGATTCTGGTGTGTTTCCGGT TCTTGCTGTTAAGGCGGGCAGGATTAGTGAATTTAATGGAAAGACAGTGAGCACCATTGGATCAAGTCAGCTGTTCATTG ACCCAGATTTTGTTGAAGCTGAGAAACTGAAGAACTGGTTTGAGAGAGAAGGAAAGAGTGTCCCTTGTATCTCCTTATCT CGGGAGTTTTCCGGCAGTGGCAAAGTAGATGTTCGTAAGACAATATCTCAAATTAAAGATGAGAAACTAGGAACCTCCGA AAAACCAGACTGGATCACAGTCAGTGCCACAATTTTATACTTGAAGTTTGATAACTTCTGCTACACAGCTTGTCCTATCA TGAACGGTGATCGTCCATGCAGCAAAAAGGTAACAGACAATGGAGATGGGACATGGCGGTGCGAAAAGTGTGATAAAAGC GTTGATGAATGTGACTACAGGTATATACTGCAGCTTCAGATACAGGACCATACAGATCTTACTTGCGTCACAGCATTCCA GGAGGCTGGTGAAGAGATAATGGGCATATCTGCTAAAGATTTGTATTATGTGAAGAATGAACACAAGGATGAGGAAAAAT TCGAAGATATCATCCGTAAGGTTGCTTTTACCAAGTACAACTTCAAGCTGAAGGTAAAAGAAGAAACATTCAGCGACGAA CAGCGTGTGAAAGCAACAGTTGTGAAAGTGGACAAGCTAAACTATTCAGCAGATACCAGGACTATGTTAGGTGCTATGGA TAAACTCAGGACCAGAGATGCAAATTCTCTTCCCATTAACCCAGAAGGATCCGATTACAATGCTGATGTGGTTAATACTG GTATAGGAAGCTCAGGAACCAGAGATCCCTCATCGGTCCAAAGAAGGGATTTCGGTTTACATGCGCATCAATCGGGTCAA TCTGGGAATCACTATAGTGGTGGTGGTGCTACTACTAGCTGCAATGTCTGTGGAAACAGTGGTCATGTTTCTGCAAAGTG TCCAGGCGCTACTAAGCCGCAAGAGCAAGGGCAGTACATGGGTGGCTCTTACAGAGGCACGACGGGGAGTTATGGAGGAG GACTACCGAGACAACATGTTGGAAGCTACTGAGAGTCATAATCATCATCATCTGATGATGCTCTCACTCTTTTTTTTTGT AGACTTTTGTTTTGGTAGAATTAACTAATGCCAAACTTTTCTGAAGTCTTT .................N...........................N.............N.................... ..................................N............................................. .........................................i...................................... ............................................................N................... ............................................................................i... .................N....................................................i...N..... .....................i.............................N............................ ................................................................................ ...........N.....N..................................................N........... ........................................................................N....... ......i.........N..................N....N........N.............................. .........................i.....................N................................ ..............................................................i................. .........i..................N................................................... ...................................N............................................ ................................................................................ ............................N.............................N..................... ...............................................................................i ................N......................N.....i......i........................... ....N...i....................................................................... ...................i...........................N........N...........N........... ................................................................................ ...............................................................i...........i.... .................N.........................................i.....i.............. ............................................................N................... ...........................................i....................i............... .....................................N.................................N........ ...............N......................................N..N...................... ...........................N....................... Pos Score Pred ------------------------ 18 0.312 - 46 0.117 - 60 0.167 - 115 0.222 - 202 0.546 Yes 301 0.390 - 397 0.640 Yes 418 0.429 - 471 0.874 Yes 475 0.455 - 502 0.562 Yes 532 0.191 - 652 0.140 - 658 0.193 - 709 0.125 - 793 0.150 - 807 0.613 Yes 817 0.339 - 836 0.357 - 841 0.348 - 850 0.104 - 906 0.732 Yes 928 0.157 - 1023 0.634 Yes 1050 0.701 Yes 1069 0.321 - 1156 0.283 - 1309 0.152 - 1339 0.261 - 1440 0.635 Yes 1457 0.246 - 1480 0.360 - 1486 0.525 Yes 1493 0.501 Yes 1525 0.400 - 1529 0.677 Yes 1620 0.735 Yes 1648 0.185 - 1657 0.251 - 1669 0.196 - 1824 0.732 Yes 1836 0.540 Yes 1858 0.278 - 1900 0.520 Yes 1906 0.504 Yes 1981 0.349 - 2044 0.740 Yes 2065 0.635 Yes 2118 0.469 - 2152 0.339 - 2176 0.496 - 2215 0.039 - 2218 0.069 - 2268 0.060 -

Explain the output -- Processing locus 339: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCTAATTATAAATGTCATATCAAAATTTATTACTATATGGTTTAACAAAAATTCTTATCTGAATTTTTTTTCTTTTTCTT ATCTGAATGCTTTCATTCTAATTAAAGATTTTAATATATGAATATTCGCACGTGGATATTTGTATAAGATATGCATTAAT CTCCGAGGCTATGTATAGTTCAGAATTTGAAGTGAAGTTTAGGAGAGGAAGAGAGATGGAGGTATTTAGACTTGGAGAGC TGAGACACCTACTGGTGACGGTTTTCTTGTCGGGGCTCGCCGAATACTTGATAAGGCCAGTGATGACTGACGTCACCGTA GCCGCGGTTTGCTCCGGCCTAGACGATTCATGTTCTTTGGCCGTCTATCTCACCGGAGTACAACAAGTGACGGTAGGAAT GGGGACAATGGTAATGATGCCAGTGATCGGCAATCTATCGGACAGATATGGAATCAAAGCAATGCTCACTCTTCCCATGT GCCTCTCTGTTCTTCCTCCAGCAATATTAGGGTACAGAAGGGATACCAATTTCTTTTACGCATTTTATGTAATCAAGACC CTATTCGATATGGTCTGCCAAGGCACCATTGACTGTCTCGCTAACGCTTATGTGGCAAAAAATGTTCATGGCACAAAAAG AATATCAATGTTCGGAATTTTAGCAGGTGTCAGCTCGATTTCCGGAGTTTGTGCATCACTATCAGCTCGTTTTTTATCCA TAGCTTCGACTTTTCAGGTTGCAGCTATATCATTATTCATTGGCCTGGTGTATATGAGGGTTTTCCTAAAAGAGAGGTTA CAAGATGCTGATGATGATGATGAAGCTGATAGTGGTGGTTGTAGAAGTCATCAAGAGGTTCATAATGGTGGTGATCTAAA GATGTTAACTGAGCCGATACTAAGAGATGCACCAACAAAGACACATGTCTTCAACAGTAAGTATTCGTCTTGGAAAGATA TGGTCAGCTTGATAAATAATAGCACTATACTCATTCAAGCATTGGTTGTTACATTCTTTGCTACCTTCTCGGAAAGTGGA AGGGGGTCTGCTCTCATGTATTTTCTGAAAGCTCGTTTTGGGTTCAACAAAAATGACTTTGCTGAGCTATTCTTGTTGGT TACCATCATTGGCTCCATCTCCCAGCTATTTATATTGCCGACTTTAAGTTCTACCATTGGAGAACGTAAGGTGTTATCAA CGGGGCTTCTTATGGAATTCTTCAACGCGACTTGTCTTAGTGTTGCATGGTCACCATGGGTTCCTTATGCCATGACCATG CTTGTACCCGGAGCCATGTTTGTGATGCCATCTGTTTGTGGTATTGCGTCAAGACAAGTTGGATCCAGTGAACAGGGGAA GGTCCAAGGATGTATATCCGGGGTAAGGGCTTTTGCTCAAGTTGTGGCTCCATTTGTATATAGTCCATTGACAGCTCTAT TTCTCTCCGAAAATGCACCATTCTATTTCCCTGGATTCAGCATTCTATGCATCGCCATCTCTTTGATGATCGGATTCCTT CAGAGTCTTCTGATAAAAGATCATCCTTCTTTGCCCTTAGTGAACATAACAATCAACAACCCTTCAAAAGCGGAAGCTTG ACAAGAAATATATCAAGAAGCAAATGTAACTGTAATATTGTACAAATTTGCTTCAAATTAAAAGTTCATTTAAAACGAAT ACATAGCTAGAAGCTGTAAA ...........N........................N........................................... ......N..............................N................................N......... ..........N............................................N........................ ..............................................................i................. .............................N................................................i. .......i.....N..N..............................N.............N..............N... ..................................................................N............. .........i.......................................N...........N.....N............ .......N........................................................................ .....................................................i.......................... ....N.....N..N..N..N............................................N............... ................................................................................ ...............N....................................N........................... ................................................................................ ...........i..................................N........N..........N....N.....i.. ...............i........i....................................................... .........N...................................................................... ............N.................................N..................N.............. ................................................................................ .......................N........................................................ .................... Pos Score Pred ------------------------ 12 0.225 - 37 0.264 - 87 0.165 - 118 0.310 - 151 0.437 - 171 0.398 - 216 0.478 - 303 0.724 Yes 350 0.340 - 399 0.746 Yes 408 0.528 Yes 414 0.435 - 417 0.449 - 448 0.245 - 462 0.299 - 477 0.463 - 547 0.168 - 570 0.648 Yes 610 0.166 - 622 0.146 - 628 0.329 - 648 0.471 - 774 0.703 Yes 805 0.362 - 811 0.320 - 814 0.385 - 817 0.271 - 820 0.176 - 865 0.192 - 882 0.394 - 907 0.190 - 925 0.283 - 960 0.617 Yes 1056 0.245 - 1093 0.355 - 1212 0.563 Yes 1247 0.397 - 1256 0.435 - 1267 0.246 - 1272 0.391 - 1278 0.615 Yes 1296 0.789 Yes 1305 0.540 Yes 1370 0.341 - 1453 0.234 - 1487 0.345 - 1506 0.416 - 1624 0.089 -

Explain the output -- Processing locus 340: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAGGCGGCTCAAGACAAAATTAAAGATTTTGCATAATCATTCACTTTTGGGGTCAATATTGAAACTTTCAAATTTGAAAG AGGAATATACGAATATTTTAAAATGTTTGGGGCTTATTTGTTATTACTATTTTTTTTTTTTTTTTTTTTTCAATGTGGCT AATAAGAAACTCTCAGTCTCAGTGGCTGACCATCCCCGCCGTAGCCATGGCAGTTTTCCGATGCGCCGTCTTTTTATCTC CTTCGAGTTTCCTTCCGTCGTACTCCACCGGAAGGAGAGTTTCTAACCCTAAATCCAAAGCTCAAGAGACAGTCATAATC GGAATCGAAACGCCGGAAAATGATCCTTCAAGCGACGTAGTTTCTGATGTAGAGGAGATTCAGGTCTTAAGTAGCTCGGT TGTGTCAAATGAGGTAAATGGTATATCAACTAAGATCTCTCCGAAGTTAGTAGCGAAGTATGGATTGTGGTTGATCGGAA TATTCGCTTTTCAAACTGTTTGTGCTGTTTTGTTTCTGGGAGACTCAACGAAATCCGAGAAAACGCCGGAAGTTTCAAGT GATAGTGAGGGTAACAATTTGGTTTTGCTAGAGGATGTTGAAATGAATGAGAAGATTGCAGAGATCAGAATGATGGCACG TGAAGCACATAAAAGTGAGGGTAAGCAAGAAGAAGATGATGAAACTGGGATTGATATTGAGAAAGAGATAGAAGCAAGAT TGTCTAACATGGAGAAACGGTTGAATTCACAGAGGAAAGGTTTGGCGGGATTGCGCGTAGAACCGTTGGATGAATCTGGA AATGATGAGAAAAGTTTGATGTTTGAGAAGAAGTACAAGTTTAAAGCTGAGAAGCCTCCGATGGGTAATGTAAAAGGCTT TGGTGGAAGTAAAGGAAGTGATGAGATTATGTCTGGGACTGAGAAGACGGGGAAAAATGGAAGTGCTAGTGAATCACGAG ACGGTGAGAAGAATCCTGAAGAACAACTACAAGAGTCAGTATTCCGAGATGGTGCTGCTCAGGATTCTGAGCAAAGACGA CCATCAAACGAAGTTAAGAAGTCAAGAATATCTGGAAATCGAGTGGGCGGAACACCAAATATGAAAGCTGGTTCTGGATT TGGTAGCACTTCATTGTCAGAGAAACATGGAGACGTCAGGAAAGGAACACCATTGAGAAGAGCAAAGGAGAAACAATCTG AGAAAGAGAATAAATTGTGGTGGCTCAAGCTTCCCTATGTGCTGAGAATTCTCATGCGGAGTAATATCGATCAAGACATA TCTGAGGGATATTTTACCTTGAGGACCGAATCTATGGAACAAAATGAGGGTCAAGTTTCACATATGATTGCATTTGAAGA TCAAAGTGATGCTAGAAACTTCTCTTATCTGCTTGAATCAGTTTTTGAAGACTTGGATGATTTCAGTGCCGATATAGCTC CCGTAACAACCAAGGATCTTTATGAGGAAGTTAGTTCTGGTGGCAAGAATGTGATAGTTGTGAGGAAGAGGCAGCTTACG TTGTATGCTGGACAACCATTTGAAGACGTTGAAAGAGCTTTGCGAACTCTGATTCAAGAACGAAGATGAAAAAAGTAACA ACATTACTTCGGACTGAAAAAACTAGAGAGCCCAGAGATGATCAATGTGGAGATGACAGGGGCAGTAAGAACAATGGTTC CACAACAA ................................................................................ ......................i.................................................N....... ..............................................i.............i................... ................................................................................ ...................N..........................N................................. ........N........N.........................................N.................... ................................................................................ ..................................N.......i...N......................i..i....... ...................................N..N......................................... ........i............................................................N.......... ....................N.......i...........................i....................... ................................................N............................... ............................................................i................... ..........................N..................................................... ....................................N................N.......................... .................................N.........N...................N................ ........N...............................................N....................... .....................N..........................N............................... ....N............................................................i.............. .....................................N......N.......N....................N...... ........ Pos Score Pred ------------------------ 103 0.581 Yes 153 0.169 - 207 0.646 Yes 221 0.518 Yes 340 0.160 - 367 0.116 - 409 0.120 - 418 0.074 - 460 0.442 - 595 0.120 - 603 0.865 Yes 607 0.105 - 630 0.695 Yes 633 0.837 Yes 676 0.209 - 679 0.072 - 729 0.587 Yes 790 0.197 - 802 0.248 - 805 0.220 - 819 0.645 Yes 861 0.764 Yes 868 0.306 - 901 0.285 - 909 0.666 Yes 937 0.660 Yes 1009 0.482 - 1101 0.731 Yes 1147 0.386 - 1237 0.140 - 1254 0.291 - 1314 0.483 - 1324 0.255 - 1344 0.444 - 1369 0.165 - 1417 0.130 - 1462 0.191 - 1489 0.429 - 1525 0.201 - 1586 0.815 Yes 1638 0.133 - 1645 0.057 - 1653 0.066 - 1674 0.043 -

Explain the output -- Processing locus 341: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGACCATAACAGAAACATGTTTTACGGGACCATGATAATTCTACATTCCCTTAGGGGAGGGTTGTTTAGTTCATGGGAAA AGTATGTGTTGAAATATGTAAATACACACCAACAAACGCACATATCATATATATATGGTATACAAGCTCACATCAAAAGT GGATTCTAACCAAAGAATAATACAACGAAAAATGTTTGCTAAAGAGAAGACAATAATGGAGGAGGACCGCGTGAGATTCA TATGCAAAAGAACCGTGGTGAGCACTAGATCGATTGAACCAGGGCGATTATACCAGTTATCAGTATTGGACCACGTCATG GAACCAAACCACATTCGTCTAGTCTACTATTACCGGTGTTCCAAGACTAGAGAACCGGGAGAAATCACAAAAAAATTAAG GGAGTCATTAGCATACACACTCAATTGTTATCCGATTGTAACCGGGAGACTGGTTAAAGAAGTTGATGGTATGGAAGAGA ATAAGGATTTAAGCCAACGATGGATGGTAAAGAGCAACGACGCCGGCGTGAGGATGGTGGAGGCCAGAGCAACCGGGTCA GTTAAGGAATGGCTTAGGAGTGTAAATAGAGAGGAAGAACTCAAGCTTGTTCATTGGGAAGACATGTATCATCTTCAATA TTATTGGTCTACCTTCTGTGTTCAGGTAACTGAGTTTGAAAGTGGTGGGTTAGCTATTGGATTGAGTTGTAGCCATCTCT TAGCCGATCCAGTTTGTGCAATGATGTTTATTCGAGCTTGGGCAGATTTAACTTTGTCTCGGTCCATGATGGCTCCACCA CTATTCCACCCGTTGCCGCCTCGCCGGTTTTCCAACCAAAGACTAATCTCTAACAACCAACTGCTTAGCCATTACATCAA ATCTTGCTCTCTAACGGCTTCACCGTCAAACATGACCGAGGACCATATGGTCACCGTTACTTTTCTGTTCCCGGATCCGC TGGTCAGAGCCGGCGAGAATGAGCCAAGGATTTCGACATTTGAGATTTTGGCTGGACTCTTTTGGGTCTGTGTGAGTCGA GCCAAAGGAAAAAGAAACGAGCTCATGGACATGTCCTTGTGCTTAGACGTTAGGAAATTGTTGCGTTTAGACCAAAGTTA TTTTGGTAATTGCATGGTTTATCACAAGGTTCCCTACTCCAAACCGGTTAAAACCAAAGATAAACTCTTATTCCACGCTG TTCAAGAGATTGAGAGCATAACCAAGAGACTAGACTACGACACGGTCATGGACTTGATTGAGTGGTTGAGTAGCAATAAC GGTGCGATATCAAATGGTTCGGATCTCGTGTGTACTAATTTAGAAAACATGTCTCATTCACGTCCGATGATGTTCGAGGA GGATTTGGCTCTGAGCCACGTATCTTGCTATGTAGAAGGGCCTGTAGCTGGAGGTGGACAGGTCATAGTGCTTCCCTCAC CGCCCGGTAAGGAACCAATGAGCCGAGTGGTTATGGTCAGTCTTCCTCAAAGAGTGATGGTTAAGGTTATAGAAGACGAG CTTCTTTTAAGCTTTTCTCCGGTCGTAATTATGGAAGATACAAAGCAATTATAATTATACTTTATATGAAAGTATTAGTT AATGTTTATACTAAAGATCAATGTAATATAGGAGCAAAGGTAATATTAACGTCTAAAACACATAAGTGGTTGT ................N..............N........................................N....... ...N...........N......................................N......................... ...............................i.......................i........................ ................................................................................ .................................................................N....i......... ...................i...i.............................i.......................... ........N......................................................N................ ................................................................................ ....................N..N.........................................N..N........... ................................................................................ ...............................i..............i................................. ..................N............................................................. ........................N.....N................................................. .............i.................................................................. ...............................................N................................ .............N..................................i.................N..i.......... .............................N.................................................. .................i..............N.......................N....................... ..............................N..................................N.............. Pos Score Pred ------------------------ 17 0.400 - 32 0.358 - 73 0.184 - 84 0.307 - 96 0.211 - 135 0.399 - 192 0.557 Yes 216 0.535 Yes 242 0.523 Yes 318 0.242 - 466 0.235 - 471 0.556 Yes 500 0.750 Yes 504 0.685 Yes 534 0.602 Yes 569 0.280 - 624 0.289 - 741 0.285 - 744 0.375 - 786 0.262 - 789 0.238 - 912 0.892 Yes 927 0.502 Yes 979 0.315 - 1065 0.383 - 1071 0.428 - 1134 0.525 Yes 1248 0.498 - 1294 0.379 - 1329 0.577 Yes 1347 0.370 - 1350 0.625 Yes 1390 0.236 - 1458 0.551 Yes 1473 0.199 - 1497 0.188 - 1551 0.203 - 1586 0.095 - 1602 0.078 - 1621 0.136 -

Explain the output -- Processing locus 342: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTTTTAAAAATATTGTAGTGGTAAATACCGAATTTCCATATAGTTTCTGTGTGGCTTATATTTGTCCTTTTATAGGGT GCAATTTTAGAGTAAAGATAGTATCTAGGTTTAATGTTAAAGATTCTAATAATGATGGGGCGTTTTCGTGTTTTCTATTT ATTTAAGCTCCATTTATTATTACCGGCTGTTTTCTATTTTGTCGTTCCCGGTAAATTTTGCCGGCGAAGATTGAAACTTT TCCAAACGATGCAAGCAACGGCGAATCTTCCGACGATATGGAATCTGTCGCTTCGTGGGTCATCCTCTAAAGTCCATATA GTGAACCCTAGGCATCGAAAGTCTCACGCGCTTCATTGTTTGAGATCAGAAGGGCATGAAGAGTTTGACAATTCTCAGAA AAGTCTTGGACAATCTTCTATTACAAAAGAAGCAAAACATAAGGACATATGGAACCTCTTTAGAGAAGCTCAAAAAAATA TAATGATCTTAAACAAACAACGCCTAGCTGCAGTTGACGAGTTAGAACAGCTAAAGAAAGATAAAGAGGAATTGTTAGAG AGGATAAACCAATTGGAGGCAGAAAGTCAGATTGTAATCAAGAAAGATAAGTCGTCTCTATTCTGGGAGTTGCTTCTCCG TATTGATTCTATGGTTATCAATGGACTAGTTAACATTGAAGAAGCTTCATCCATGAGAAAGCTGGTTAAAGAGCATGAAG CGAACATCTCTGTATTTCCTCTTGATGTCCTGCAACAAGGAGATGCCGAAATTCTAGCAGAACTTAGACGGTTCACAAAT AAAGGCAAAAGGAATGGTCTCCATGTGATTCATATTTGCACCGAAATGGCTCCATTAGTTTCTGTTGGACCTTTGGCCTC ATATATAACAGGATTGTCTTGTGCACTCCAAGAAGAGGGTTACATGGTTGAAGTTATATTGCCAAAATATTCTACACTTG ATTTGGACGAAATTGAAGGGCTTCGTGAAATCGAAGCCGATGCATATTCTTATTTTGATGGCCAGCTGCATGCAAACCGA ATATGGAACGGAGTTGTCTCTGGAATAGGAGTGACACTTATTCAACCAGTGTATTACTCGTCAATGTTTAGCCGTGATAA AGTATACGGGTATCAGGACGATTTTGATAGATTTGCATACTTCTCTCGTGCTTCATTGGATTACATTGCAAAATCTGGAA AGCAGCCTGATGTTTTACACATTCACAACTGGCAAACTGCAATAGTTGGACCGTTGTTTTGGGATGTTTTTGTTAATCAG GGACTTGAAGGTACTAGAATACTCTTGACATGCCAAGACTTTGACAAGGGTCTTGTACCTCCTGAAAAATTGGAGCTATG TGGGCTCGATCCAGCTGAACTTCACCGTCTTGATCGCTTACAAGATAACACAAATCCACATTTTGTTAATATTTTGAAGG GCGGTGTTGTCTACTCCAACAAAGTTGTAATAATGTCATCCTCACATAGCTCAATCCCTGGTCTCGAACCAACTTTAGCT ATCCACAAGGACAAGTTGTTTTTTGCTCCCTTTGGAATGGACAACTCAATGGAGAAAGACTTGTGTTGTGACCTTCACGT CTCTGCATATACTTCCATTAAGAACCTATGAAAATACACAATGATGAACAAGATGAATGATTATTTATCATATTTTCACA TTAAAAAAAAAAATTGTGTCCTTGTGTTTAGTGAGAATAGTTGTATCTAC ................................................................................ .................................N.................i..N......................... ................................................................................ ........N............................N.......................................... .......................................................N........................ ................................................N............................... ..i............................................................................. ................................................................................ ..........N.........N...............................N.....................N..... ........................N.................N..................................... .............i........N......................i.................................. ...........................................i.................................... .......................................N.................N...........N.......... ..N............................................................i................ ................................................................................ .........N.....................................................N................ .............................N...............................................N.. ................................................................................ ................................i............................................... ....................................N...........N............................... ...........................N............N..N........N...N....................... .................................................. Pos Score Pred ------------------------ 114 0.188 - 132 0.526 Yes 135 0.337 - 249 0.454 - 278 0.475 - 376 0.241 - 449 0.452 - 483 0.743 Yes 651 0.201 - 661 0.254 - 693 0.400 - 715 0.416 - 745 0.298 - 763 0.113 - 814 0.731 Yes 823 0.266 - 846 0.672 Yes 924 0.748 Yes 1000 0.260 - 1018 0.146 - 1030 0.093 - 1043 0.305 - 1104 0.546 Yes 1210 0.192 - 1264 0.234 - 1310 0.400 - 1358 0.235 - 1473 0.733 Yes 1557 0.371 - 1569 0.486 - 1628 0.216 - 1641 0.255 - 1644 0.140 - 1653 0.109 - 1657 0.102 -

Explain the output -- Processing locus 343: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACACTATACATCAGTTCTTTCTTTTGAAGTAGTGAGCTGAATCTAGTTCTTTAGCTGTTGGAAATTTTCAGATTTGTGT CTCTGATGACAGAGAATTTGATACCTTTTTTTTTGTTGTGAAATGGATTTAGGCATGAATGAGTCAGTCCCTGATTCTGT GGAAGACAACGGTAATTCAGTCCCTGCAAATGGATTATTAGTATTGCCTGATAATGATCATGAGGAAGGAGGTGTTGGAT CACCTCAACGATCGAACAGCGTTGAATCTCCTGGTGGATCAGTACATTCTACGAGGAAAGGTTTTGGTTTGAAGAAATGG AGACGGATCAAAAGAGATGGTCCTGTGAGAGATGAGGCTGCTCCTGTTGATGATGGTAGTAAGTTACTGAAACGTGGATT GGCTGGTTTGGTGAATCCTCCTTCTAAACATGTAGAATTTTCTTCAGTTGAAGCAAGGCAGAGTAGTGAAGGCTCTGTTG GGTCAGTGAATATGGTGCATCATCCAGGGGTAGCCAACGGGTTTAGTCCCGATATAGGTTGTATGTTTGCTGTTGGGCAA GCGTTTGAGAAAAGTGAAGAGCATAGTGGTAATACCATAGGTGGAAAGAATGTAGTAGGAGGGAAGGTTGTTTCAGGTAG CCAAGAGAAACTCTGGAGTGATACCATCAAGAGGGCTAGCGAAGAGCGGGGGGATATCGAGAAGGAGAAGCCTTGTTCCA GCTTGGATTCCGACTTGAGAAGTAGTGATTTTGTGTTTTCGACTGGCTCGGTTTCTGTTGGTAACCATGGAGAGAAAGAT GAGAGATTGACGATGAATTATATTGGGGGATTTAGCAATGAAGGTCAAGTTAAGGAGGAAGTTCAAACATATAGCAGAAG CGAAAACGGGAATAAAGAAGACGATGGAGAGAGTAAGAAAAACAACAATCATTGGGCAGATAAAGACGCACTTGCAGATT CTATCAGAAGCTTTGCAGTTCTGCAGGAAGTTCTTTGGAAAGAGGTTCAAAGTTTCCAGGAGCTAGGTAAGGAATCTGTA CTACTACATTCGAACACCGATGAATTGAGTTCAGACCAGCCAAGTCATCAAAATTGTAAAGAAGATAACTCAACATCCTC AGGATCAAAGGCACTGATCTTGAAGGAGAAGGTAAAACTTCTGGAACACAAACTCGAGGAAGCGAGAGCTGCTCTCGAGG CAAAGGAAGCTAGGATCCAAGAACTCGAAAACTCAAAGATTGAATCTGAACTTGAATGCATCTTCCAGAGAAAGATTGAA ACTGAAATCGAACATTTGATGCTCACAAGATCTCTGAGTTCACTACAAGTACTCCAAGAAACTAAGAAGTTACATTCTCT GAAAGAAGATCCTGTATCAAACCGTGGAAACATCTTAGGAAAAACATGTAAGCTCGGTTTCTATATCTTGACACAATTGA TCTTGCTCGTCTCTATACTTCGGTTTCTCGTCCTACAATTCTCTCCTGCTTCACGATTAGTAATACCAACTTGAAAAATT CCTTCTCCTTGTTGTGTTACCGAACATTTTTTTTGTTTCTCCAGATTGTAATAAATAAGGTCTCTCAAGGATATTGTCCC CATTGTTATAGTT ................................................................................ .....N....................................i...........i...N..................... .............................N.......................N.....N.................... ............................................................................i... ................i..............N.................N..N........................... .............................N.................................................. ...........i..................................................i................. .................................................i.............................. ................................................................................ ..................................................................N...........N. ............i........................i.......................................... .......................N........................................................ ................................................................................ ...................N............................................................ ................................................................................ .......................................................N........................ ..................N............................................................. .............................................N.................................. ................................................................................ ................................................................................ ............. Pos Score Pred ------------------------ 86 0.118 - 123 0.692 Yes 135 0.595 Yes 139 0.466 - 190 0.353 - 214 0.309 - 220 0.291 - 317 0.585 Yes 337 0.522 Yes 352 0.172 - 370 0.065 - 373 0.260 - 430 0.284 - 492 0.642 Yes 543 0.653 Yes 610 0.559 Yes 787 0.371 - 799 0.248 - 813 0.744 Yes 838 0.535 Yes 904 0.271 - 1060 0.186 - 1256 0.334 - 1299 0.120 - 1406 0.357 -

Explain the output -- Processing locus 344: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATTGTATCAATAAATAGACATAGTTGATAGTTCCTTAATTTCATTCTGAATAAGATCAAGAATTTACAGGTTCTCTCT TCTCTCTCTCTCAAGTTCTGATTTGTTTATACAAATTACAACTTTAGTTTCGTAAATAGGTACTCATTAGTTGATCATTG CTCTCTATGTCTTGCTATGCAATTCAATAGGCTAGTGTATCACCAATCACAGATCCGGGATCCCGGCTTAGTAAACATTA TAAAGCTTGAATATACACTTTATTTGGATTTGGATAATTTAGTTAAAAAATTCATTTTACAGTATTTGAAAAAATACAAA ATGGCGAAAATCTACCGGAAGTTGACAGGCTACGGTGGTGAAGGAGGGCGTGAATGGGACGATGATGTATATGAGGGTGT AAGAAAAGTGTATGTAGGACAAGATATCAATCGTATCACTTACGTCAAATTCGAGTATGTGAAGGAAGACGGCCAAGTCG TAACAACTGAATATGGGAAAATCATTCAACAACCCAAAGAGTTTGTACTTCAATATCCGGACGAACATATCATAGCGGTG GAAGGAAACTATCGCGGAGTGGCTCTATGTGCCACAGAGGTGATCACAAACCTCGTCTTCAAGACCTCAAAGGGTAGAAA GTCACCACTGTTTGGTCCAAACTTGCTTGGAATTACGACCGGTACAAAGTTCGTTATTGAGGATGGAGGAAAGAAGATCG TAGGGTTTCATGGACGGTCGGGTAATGCTCTCGACGCGCTTGGAGTTTACTTTGTACATGGCTCTCTAACAACGTCTCCG CCTGTTTACAAGCTGGATGCCCAAGGTGGTACAGACGGGCGTGTTTGGGATGATGGTTCTTACGACGGCGTTAAATCGCT GCGTATTGGTCAAGATAATTCTCGTATTACTTATTTAGAGTTCGAGTACGAGAAAGGCGGTAAGTTAGAGACATGTCGCC ATGGGGTGAAACAAGAAAGATCACTGAAGTTTGAGCTTAACCCGGATGAATACATCAAATCGGTGGAAGCAACCTATGAT AAACCGGACATTTTCCGCAATGTCGTCATTACATCGCTTACATTTGAAACATCGAAGGGGAGAACGTCATTCTCTGGGTA TAAGGGAGGTAAGAAGTTTAAGCTAGAGCAAAAGGGTCGTAGGCTTGTCGGGTTCCATGGAAAGGAAGGTTCAGCTATTG ATGCCCTTGGAGCATATTTTGCACCTATTCCTACTCCGACTCCTATAATTCCAGCCAAGAAACTACCAGCAATAGGCGGC AGCGGAGGAGTTGCATGGGATGATGGTGTCTACGATGGTGTAAGGAAGATACTTGTAGGACAAGGTAACGATGGTGTAGC CTTTGTCAAGTTTGAATACAATAAAGGAAAAGATCTTGTATCTGGAGATGACCATGGGAAGATGACATTACTCGGAACTG AAGAGTTTGTGCTTGAAGATGGTGAATATCTCACGGCCATAGATGGCTATTACGATAAGATTTTCGGAGTCGAGACACCA ATGATTATCTGTCTTCAGTTTAAGACGAACAAAAGGGAGTCAACTCCATTCGGAATGGATTCGGGTGAGAAGTTCTCGCT TGGAGAGAAAGGCCACAAGATCGTCGGATTCCACGGACAAGCTAGTGATGTTGTTCACAGCATCGGAGTCACTGTCGTGC CCATTACCAACACCAAGTGAAAAACCTTTTATTATATAAGTAAAACCAGCCAGTGCCATGTCGACTAAATGAAAACATTC AATAAGGATTGGTGTGTTTACTCTACTACTTTTGATGTG ................................................................................ ................................................................................ ......N.........N............................................................... ................................................................................ i....................................................N.......N..N.....N......... ...........N............................................N....................... ............N................................................................... ..........................i..................................................... ..............................................................N................. .........N..............i................................i...................... ................N................................N..N........................... ........................................................................N....... N............................................N.............................N.... ...................i............................................................ ........................................................N....................... N............................................................................... ..............N....N..N...........N...................................N......... ...............................................N.....N.......i.................. ..................N.......................N..................................... N.....................................................i......................... ...............................................N................................ .........................................................N..........N........... ..................................N.... Pos Score Pred ------------------------ 167 0.328 - 177 0.319 - 321 0.881 Yes 374 0.149 - 382 0.184 - 385 0.149 - 391 0.184 - 412 0.289 - 457 0.262 - 493 0.208 - 587 0.561 Yes 703 0.442 - 730 0.267 - 745 0.564 Yes 778 0.549 Yes 817 0.243 - 850 0.292 - 853 0.245 - 953 0.490 - 961 0.298 - 1006 0.142 - 1036 0.335 - 1060 0.517 Yes 1177 0.283 - 1201 0.214 - 1295 0.078 - 1300 0.114 - 1303 0.210 - 1315 0.331 - 1351 0.236 - 1408 0.317 - 1414 0.165 - 1422 0.685 Yes 1459 0.176 - 1483 0.247 - 1521 0.497 - 1575 0.766 Yes 1648 0.446 - 1738 0.085 - 1749 0.057 - 1795 0.028 -

Explain the output -- Processing locus 345: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAATGGCGTCCCAACAAAAATGATTTTACAGTTATATTACTTTTAAGAGTTCCTTATTTTAAAATTTGATATAAAATCA TAAAATAAAGTTTTGGATTAAATCATAAAGCATCTTCCACTATTCTCTGGATTTTGTGATGCGATGCACTTACCATCTTC CTCTTCCTTGTACTCGTCTCCTACGAAGAGTCTATTCGCAATGTTCTTGAAAGCTCGAGCTTTATCAGTAACTACACAGA ATCCTCCTGATCTCTTCTCGCTTCTACATCATTCTCCAAACGCTAAACATCTACGCCATCTCCACGCTCACCTTCTTCGT ACATTTCTCTATTCCAACGTTGTGCTCAGCTCCAAGCTCGTCCTCGCTTATTCCAAGCTGAACCATCTCTTTCCTACTTC GCTTTCTGTCTTCTGGCACATGCCTTATCGCAACATTTTCTCTTGGAATATAATCATCGGTGAGTTCTCTAGATCGGGTT TTGCTTCCAAGTCAATTGATTTGTTTCTTCGTATGTGGCGAGAGTCTTGTGTTAGACCGGATGATTTTACGCTTCCTCTT ATTCTACGAGCTTGCTCTGCGTCTAGGGAAGCGAAATCTGGAGATTTGATTCATGTTCTGTGTTTGAAACTTGGGTTTAG CTCAAGTTTGTTTGTTAGTTCGGCTTTAGTGATAATGTATGTAGATATGGGGAAACTTTTACATGCACGTAAGTTGTTCG ACGATATGCCTGTGAGAGACTCTGTTCTTTATACAGCTATGTTTGGTGGGTATGTTCAGCAAGGAGAAGCTATGTTGGGT TTAGCCATGTTCAGAGAAATGGGGTATTCTGGATTTGCGCTGGATTCAGTGGTGATGGTGAGTTTACTTATGGCTTGTGG ACAACTTGGAGCGTTGAAGCACGGGAAGAGCGTCCATGGATGGTGTATCAGGAGATGCTCCTGCTTGGGCTTGAATCTCG GCAATGCTATAACAGATATGTATGTAAAGTGCTCGATACTGGACTATGCGCACACTGTATTTGTAAATATGTCGAGACGA GATGTGATCTCTTGGAGCTCGCTTATTTTGGGGTATGGTTTGGATGGAGACGTGGTAATGTCTTTTAAACTCTTTGATGA GATGCTTAAGGAAGGAATTGAACCCAATGCTGTCACTTTTCTTGGTGTCTTATCAGCCTGTGCCCATGGCGGTCTTGTGG AAAAATCTTGGCTGTATTTCAGACTAATGCAGGAATATAATATAGTTCCTGAACTGAAACACTATGCTAGTGTGGCGGAT TGTATGTCTCGAGCAGGTCTTCTGGAGGAAGCAGAGAAGTTTCTGGAAGATATGCCCGTGAAACCTGATGAGGCTGTTAT GGGTGCGGTGTTGAGTGGGTGCAAGGTGTATGGAAATGTAGAAGTTGGGGAAAGAGTGGCGAGGGAGCTGATTCAACTTA AGCCGAGAAAAGCTAGCTACTATGTAACGTTGGCCGGTCTATATTCAGCTGCAGGTCGGTTTGATGAAGCTGAGAGTCTG AGGCAGTGGATGAAGGAGAAACAGATTTCTAAGGTTCCCGGGTGTAGCTCAATATGAAAGATGGGATGGAGATTTCAGCG TCTTCTGGTTGTACATGTGAAAGATTACCAGAATGGTAAAGATCAAAACCGCACCAAAAGCTCTTGAAACTTACAT ...N................N........................................................... ..........................................................N....N................ ........................................i....................................... ................................................................................ ................................................................................ ...................N............................................................ ................................N...........................N................... ....................................................N........................... ..................................N...N.......i...............N................. .....i................................N............N...................i........ ......i...........i...................................N..............i.......... ...................................N...N..............N......................... ...N.............N...N.......................N......................i........... ..........................i....................................N................ ...i...............................................i...............N..........i. .............................N.....N............................................ .....................N.........................................N................ .........N...........................................N......N....N.............. ..............N.................N........................................... Pos Score Pred ------------------------ 4 0.446 - 21 0.359 - 139 0.481 - 144 0.159 - 201 0.554 Yes 420 0.488 - 513 0.451 - 541 0.084 - 613 0.140 - 675 0.366 - 679 0.161 - 687 0.711 Yes 703 0.179 - 726 0.634 Yes 759 0.303 - 772 0.156 - 792 0.654 Yes 807 0.526 Yes 819 0.698 Yes 855 0.419 - 870 0.536 Yes 916 0.460 - 920 0.269 - 935 0.456 - 964 0.174 - 978 0.371 - 982 0.194 - 1006 0.272 - 1029 0.734 Yes 1042 0.176 - 1075 0.097 - 1084 0.224 - 1098 0.613 Yes 1117 0.130 - 1122 0.262 - 1147 0.279 - 1186 0.193 - 1227 0.732 Yes 1264 0.185 - 1284 0.567 Yes 1332 0.688 Yes 1348 0.070 - 1359 0.601 Yes 1390 0.137 - 1396 0.228 - 1462 0.150 - 1504 0.253 - 1530 0.358 - 1574 0.310 - 1581 0.248 - 1586 0.190 - 1615 0.122 - 1633 0.070 -

Explain the output -- Processing locus 346: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGTTTAGTACGTAAGTCAATGAGAGTACTTGCAGCCTAATCATCCAAAAGCAACTTGCAAATGAAATGTGCTCCCCAC TCTTTGAAACCTTCAATATAAAGAACATCAATGATTATAGAACAAGCAAACAAAAAAAGTTTCAAGCTTTCAGACAAAAA CAAAATGAAGACTAATTTTGTTATTCTACTTCTTCTCCTCTGTGTTTTTGCGATCAGCCCTAGTCAACAAGAAGAGATCA ATCAACATAATCCAGGGATATATCATCAAAAGCTGCTATACAAGGTCCAACAATGGAGAACATCTTTGAAAGAATCAAAT TCTGTAGAACTCAAGCTATCTTTAGCAGCCATTGTGGCTGGAGTGTTATACTTCCTTGCTGCCTTAATCTCGAGCGCATG TGGAATCGGCAGTGGAGGTTTATTCATTCCAATCACGACTCTAGTTTCACGGCTAGACTTGAAAACAGGGAAGCGTTTCT TGGGCCAATACTTGATATGGGTGATATTATTGTTGGGGCAGCTCCATGAATGCAAAAGCTGCATTGAGAAAGAAAGAGTA GCTTTGTTGGATTTTAAGAAATATTGGATGTCCATTACGCAAGAATCGGACTTGGACTATGTTTTCCCTACTTGGAATAA TGACACAAAGAGCGATTGTTGCCAGTGGGAGAGCATTATGTGCAATCCTACAAGTGGGCGGCTTATCAGGCTTCACGTTG GTGCTTCGAATTTAAAAGAGAATTCTCTACTAAATATTTCTTTACTGCATCCCTTTGAAGAGGTTCGAAGTCTAGAATTA TCAGCTGGGCTCAATGGCTTCGTTGATAATGTTGAAGGTTATAAAAGCTTAAGGAAATTAAAAAATTTGGAGATTCTGGA TCTCTCTTATAATAATAGATTCAACAACAACATACTTCCATTTATTAATGCTGCTACGTCACTTACAAGCCTTTCTCTTC AAAATAACTCCATGGAAGGCCCTTTCCCTTTTGAAGAAATAAAAGACTTGACAAACTTGAAACTATTGGATCTTAGTAGA AACATACTAAAAGGCCCCATGCAAGGGTTGACTCATCTGAAAAAGCTAAAAGCTTTGGATCTAAGTAACAATGTTTTTTC GAGCATAATGGAATTGCAAGTAGTTTGTGAAATGAAGAATTTGTGGGAGCTTGATCTTCGTGAAAATAAATTTGTAGGTC AGCTTCCTCTTTGTTTAGGTAGATTAAACAAGCTAAGGGTTCTTGATTTGTCATCCAACCAGCTAAATGGAAATCTACCA TCTACTTTCAACAGACTCGAGTCCCTTGAATATCTATCATTATTAGATAACAACTTCACCGGCTTCTTCTCATTTGATCC ACTCGCCAACCTCACAAAGCTTAAGGTGTTCAAACTTTCATCAACATCAGATATGTTGCAAATCAAGACAGAGAGTGAGC CAAAATATCAATTTCAACTAAGTGTTGTTGTAATAAGAGTTTGCAGCTTGGAGAAAATCCCTTCCTTTCTGGTTGTTGGC AAACAATCCGGAACTTAAAGTATTACAGTTGCAGGATAATTTATTTACCATCTTCCAGATGCCTGCTACAATAGTGCATG AGTTGCAGTTCTTGGATTTTTCAGTGAATGATATTAG ....................N.........................................i....N............ ..............................N................................................. ....N........................................................................... ....................................................i........................... .............................................................................N.. ................................................................................ ................i............................N...N.............................. ...........................i..............................i....................N .....................................N.......................................... ................................................................................ .............N..............N................................................... ...............................................N................................ ...........N.................................................................... ..................N...................................................N......... .......N.......................N................................................ ..................................................................N............. ................................................................................ ....................................................N........................... ................................................................................ ..........................................................N..................N.. ...........................N......... Pos Score Pred ------------------------ 21 0.358 - 63 0.529 Yes 68 0.488 - 111 0.328 - 165 0.362 - 293 0.629 Yes 398 0.260 - 497 0.621 Yes 526 0.145 - 530 0.197 - 588 0.511 Yes 619 0.582 Yes 640 0.341 - 678 0.338 - 814 0.225 - 829 0.202 - 928 0.107 - 972 0.472 - 1059 0.497 - 1111 0.121 - 1128 0.279 - 1152 0.224 - 1267 0.382 - 1413 0.371 - 1579 0.064 - 1598 0.062 - 1628 0.029 -

Explain the output -- Processing locus 347: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGTCACATAAGACTTTTCTCATCCACCGGTCTTGTAAGAGACAGACAGTGCAAAGATTGTCAATTATTCCTAATCCATC TTTTATACCATTGATACTTAAAATGGTTTAGCTCTTATATATAAAAGCTTGAGTATGTTCTTTCTTTTACTAAAACTTAT AACATCTTCAAAACATTCTAAAATGGCCACTTCCGTCAATGGTTCCCATCGTCGTCCACATTACTTGCTTGTAACATTCC CAGCGCAAGGTCACATCAACCCGGCGCTTCAACTAGCCAACCGCCTCATCCACCACGGTGCAACCGTCACATACTCCACC GCAGTCTCTGCTCACCGACGTATGGGCGAGCCACCTTCCACAAAAGGTCTATCCTTCGCTTGGTTCACCGATGGATTCGA CGACGGTCTCAAGTCATTCGAAGACCAGAAAATCTACATGTCCGAACTCAAACGATGTGGTTCAAACGCCCTGAGAGACA TCATCAAAGCCAATCTTGACGCCACCACCGAAACAGAGCCTATCACCGGGGTAATCTACTCTGTTCTCGTCCCGTGGGTT TCTACGGTAGCGCGTGAGTTTCACCTCCCAACTACACTTCTCTGGATTGAACCAGCTACTGTACTAGACATCTACTACTA CTACTTCAACACCTCTTACAAACATCTCTTCGACGTTGAACCGATTAAATTACCGAAACTGCCACTGATCACCACCGGTG ACCTCCCGTCGTTTCTTCAACCTTCGAAGGCATTACCGTCAGCTCTTGTGACTCTAAGAGAACATATCGAAGCTCTCGAA ACGGAATCAAACCCTAAGATTCTTGTTAACACATTCTCTGCTTTGGAACACGATGCTTTAACCTCTGTTGAGAAACTCAA GATGATCCCAATCGGACCGTTGGTTTCTTCCTCCGAGGGTAAAACCGATCTTTTCAAATCTTCCGACGAGGATTACACGA AATGGTTAGACTCGAAGCTCGAGAGATCAGTGATTTACATTTCCTTAGGCACACACGCCGATGATTTACCAGAGAAACAC ATGGAAGCGCTTACTCACGGCGTGTTAGCTACAAACAGACCGTTTTTATGGATCGTGAGGGAGAAAAATCCAGAAGAGAA GAAGAAGAATCGGTTTCTTGAATTGATCAGAGGAAGTGATCGAGGATTGGTGGTGGGATGGTGTTCTCAGACAGCTGTTT TGGCGCATTGTGCTGTGGGATGTTTTGTGACTCATTGTGGTTGGAATTCGACGTTGGAGAGTTTAGAGAGTGGTGTTCCG GTGGTTGCGTTTCCGCAGTTTGCTGATCAGTGTACAACGGCGAAGCTTGTGGAGGATACGTGGAGGATTGGAGTGAAGGT GAAGGTTGGGGAGGAAGGAGATGTGGATGGGGAGGAGATTAGAAGGTGTTTGGAGAAGGTGATGAGTGGTGGAGAAGAGG CGGAGGAGATGAGAGAGAATGCAGAGAAGTGGAAGGCGATGGCTGTTGATGCGGCAGCGGAAGGTGGACCGTCGGATTTG AATCTTAAAGGTTTTGTGGACGAGGATGAGTAGTGGGTTTTAAGTTTTTGAAGTTAAAGAATGTGCATCTTACCTTGTTC AAATAAAGAGAACAAACAGAGAAGAAGGTCTCGTTTATTCACTCTTGTACTC ................................................................................ ......................N...............................N......................... ......................i...............N......................................... ................................................................................ .....................i................................................i......... .....................................i................i......................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ....................................................N........................... i..............................................i................................ .........................................................N...................... ...................N............................................................ ................................................................................ ....................N.....N..................................i.................. ........i.........N...................N.........N............................... .........................N..................................N................... .................................................... Pos Score Pred ------------------------ 103 0.423 - 135 0.354 - 183 0.859 Yes 199 0.478 - 342 0.764 Yes 391 0.727 Yes 438 0.879 Yes 455 0.579 Yes 853 0.302 - 882 0.808 Yes 962 0.613 Yes 1021 0.233 - 1041 0.563 Yes 1088 0.512 Yes 1178 0.246 - 1220 0.354 - 1381 0.404 - 1387 0.090 - 1422 0.730 Yes 1449 0.714 Yes 1459 0.096 - 1479 0.499 - 1489 0.097 - 1546 0.348 - 1581 0.098 -

Explain the output -- Processing locus 348: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGTGTTACATCTCCAAACTCTGATCGCTGGAAAAGTATCAAGCTTTCTGCTTGATTCTCTTTCTTAGCATCTGTCAAGC TTTCTTTGAAGATAAATTTGAGGTAAAAATCAAACTAATGGCTTCTCTCACTTTTTGTTTCTACTTCTTCTTATGATCTT TTGTCTTTGTTTTGATCAGATGGAGCAGTTCAAAGAGAGGAAGTTTGTTTGTAAGTTCTGTAGCAAGAGGTTTCCTTGTG GGAAATCACTTGGAGGTCACATCAGAACTCACATGAATGAGAATTCAGCTGATTCAGATGAAGATGAGGCTGATAAGCTC AAGATGATTGATGAAAATGGTGGTCAATCTAGTTATGGTCTGAGAGAGAATCCTAAAAAGAACAAGAGGTTTGTGGATCA CAGACAGATTATGGCTCTGAAACAGCAGCAGCAGCAGCAACAACTTCAAGAGCTGCGTCGTTGCAGAGAATGTGGTAAAG GTTTTGTTTCTTCTAAAGCTCTTTGTGGTCACATGGCTTGTCACTCTGAGAGAGAGAAGATTGTTATGGATAGTCAATCT GATACTGAGGCTTCTTCCTCTCCTATCAGAAGAAGATCCAAGAGAGTTGTTGTGAAACCTCACCACAAAGCTGCTTTTGT AGTTGGTGGTAATGGTATTATGAATCAATCTATTTCAGCTTCTTCTGATGCTTCTGAGATTGAGCCTGAACAGGAGGAAA TGGCAAGGTCTTTGATGATGCTTTCGAGGGATTCTAGTTTTAAGAAAGGACATAACTCTTTGGCTGAGTCATCTGACAAC AACTCTGTGATTCTTGAGACTAAGTCATCTTCAGGTGAGCAGGTTAAGATGTTCAATGTGAAGAATGTTGAAGAGTTATG TAAGAAGAACAAACTTGTAGTTGATAACCAGATGAGGTCTGGTGAAGAAAATGGTGATGTTCATTATGATTCAGATAATT CTGACTCTGGCTACTTCAGGAATGGACCAAAGAAGTTGGACTCAGATGTTTCTGTTGATGGGTTCTTTAGGAACAAAACA ATAATGGGATCTGGATCCGGATTCAACAGCTCCCCAACAAAACAAGATAGGGACATGAACAGATTCAGGAAGGAATGGTA TAAGGAAGGAGGGTCAGGGTCAGGATCAGGATCAGGTCGTTCTTCTACAAAGTATGATCTGAGGAAAAGCAAAAGAGGTT TTCCTTCTCATGGTCGTAAGAAAATCAAGTATGAGTTCACTGAATCTGTCTATGACAGTGGTGAGCATAGCTTGGAAACT GACTCTTGTGCGGACACAAACAGAACTATCAAGATTCATAGTAAGTCTCCAATGGTTAAGAAAGCAAACGGTGCAAAGAA GAAAAGCAAAGGTCACGAGTGTCCGATTTGCTTCAGGGTGTTCAAATCAGGACAAGCTTTAGGAGGTCACAAGAGATCAC ATTTCATTGGGAATCAAGAACACAGGACTTTGGTGATTCAACATCAAGTAGCTCATGAGATGCATACACTCATCGATCTC AATCTTCCTGCTCCTATTGATGAATGATCTTACTAAAACCGAAACCGCGGTTTTCTTCTCTTTATTGTTTCCTTTCTGTT TATTGATTCTTTTGCTTCAGATTCAAGAATGCTTTTGGTTTGCAAG ................................................................................ .....................................N..................................N....... ...................i............................................................ ................................i...N....................N.....N................ ...i......N.....i.................N............................................. ..........N..........................................................N.......... ................................i................................i.............. ................................................................................ ...........N.......N...........................N...............................i ..............N..i.............................................................. ................................................i......N........N............N.. ...............................N..................N.....N........N.............. .....................N.......................N...........N...................... ...i..................................................N...................N..... .....................................................N.......................... .........N....................i....................N............................ ...................................................i............................ ................................................................................ ......................................................N....N.................... ...................N...N........................................................ ............................N................. Pos Score Pred ------------------------ 118 0.477 - 153 0.155 - 180 0.610 Yes 273 0.566 Yes 277 0.250 - 298 0.127 - 304 0.283 - 324 0.780 Yes 331 0.159 - 337 0.524 Yes 355 0.219 - 411 0.424 - 470 0.210 - 513 0.733 Yes 546 0.627 Yes 652 0.119 - 660 0.399 - 688 0.063 - 720 0.858 Yes 735 0.454 - 738 0.596 Yes 849 0.606 Yes 856 0.159 - 865 0.224 - 878 0.114 - 912 0.473 - 931 0.209 - 937 0.135 - 946 0.256 - 982 0.445 - 1006 0.384 - 1018 0.257 - 1044 0.806 Yes 1095 0.442 - 1115 0.238 - 1174 0.230 - 1210 0.223 - 1231 0.630 Yes 1252 0.167 - 1332 0.551 Yes 1495 0.130 - 1500 0.189 - 1540 0.103 - 1544 0.237 - 1629 0.084 -

Explain the output -- Processing locus 349: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCAGGGTCATTTCCATTTTTTTATCAAAATTGTACAATTTCTTTTATTTTATTCGTATTTGAGTGGTTTCATGTATCATG TATTATATAATATTTTTATAATATAATATAATTGATTCGGTGTTAAAAAAAAAAAAACATATTAAACGGAAATTGCGAAC GTTAATGGAAAAGAATATATTTTATTATTATTATAAATGCTGTTTGGTACAGGTGGCTTACAAAAATCCAAAGTGCCGTA ACTTATTAGCTTCTCTTTCATTTCAAGTCAAAAAGTCCAAACCTCTGCAGATTTTATTCCTAAACCCGACCATGGAAGAA TCATCAAACCAACAAATCCTCAACTCCGAGACCCGACCCGACCCGATTCAAAAACCACCGGAATCACCAGCCACCAAACT CTCATTCCCGAGACTCTTCCTCTGGTTTGATCAATCAAATCGCATCAAAACTCTGCTCTCATGGTCCATCTTCTTCCTCC TCGCCGTCATCGTCCCAATGATCTCACACTTCGTGCTAATCTGCGCCGATTGCGATTTCAAACACCGTCGTCCTTACGAC GGTTTAGTGCAGCTTTCGTTATCGATCTTCGCCGGAATCTCGTTCGTTAGTCTCTCAGATTGGTCCAAGAAATATGGAAT CCGAAGGTTTCTCTTCTTCGATAAGCTTAAAGATGTTAGCGATAAAGTTAGGATCGGATACGAAGCTAAAATCCAGAGAT CAATGAAGCTACTAGCTATCTTTGTCCTTCCATCGACAACGCTTCAAGCTATCTATCGCATTTGGTGGTATGCTTCAGGC TTTAACCAAATCCCTTACATTATAAACCCAACGCTGAGCCATGTCTTAGCCTGCACACTCCAGCTCTCTTCTTGGCTCTA CCGTACATCACTCTTCATCATTGCTTGTATCCTCTACCAAAACATCTGCCACCTCCAGGTCCTCCGTCTTGATGAATTCG CACGCTGCTTCGCCTCTGAGATCAAAGATTTCAGTTCCATACTCGCCGAGCATCTCAAAATCCGTCGTGAACTGAAGATT GTCAGTCACCGGTTCAGACGATTCATTCTTTTGTCATTGTTTTTCGTCACTGCCACTCAGTTCATGGCATTGCTGACTAC CATCAGAGCTAGTGTTCCCTTTAATATCTACGAAGTTGGCGAGCTCGCGTTATGCTCCACAAGCTTGGTGTCAGGACTAT TCATATGCTTGAAAAGTGCAACACAAATGACTCACAAAGCTCAATCAGTAACGAGCATCGCCACAAAGTGGAACGTGTGC GCGTCTTTAGATACATTTGATGTTCTTTATGACGGAGAGACTCCTAAATGTCCAACAACTACACAACATTCCCAGATTTT ATCGCGTCGTCGTAATGTTGTTCAATCGTCTGATGATGATGAAGAAGGAGAAGGAGATGACAATGATCTTGAGATACATC CAATCTTTGCCCGCGCCATTTCTTCTCAGAAACGTCAAGCTCTAGTGACCTATCTAGAGAACAACAGAGCAGGGATCACT GTTTACGGATTCTTGGTGGATAAAACATGGTTGCGTATGATCTTCAGCATCGAACTTGCTCTTCTTCTATGGCTGCTCAA AAAGACAATCAGTAATTTTATATGTTTCTACTCTGAATCCATATTTTCATTTTTCATCACTTTCCTCTTGTAACCGATAA CATTCTTCATGTTTTTGGTTTTTGGCAGTGAACATAACATGAAGACAGAATCAGTGTCACACAACACACACGAAGAGATT GGTACTAAGATG ......................................................................N......N.. ................................................................................ ....N...............................N........................................... .......................................................................i........ ................................................................................ ............................................................i................... .................N.............................................................. .........................................................................i...... ................................N............................................... ..N..................................................................i.......... ........................................i....................................... .......................................................................N........ ................................................................................ ...............................................................N................ ...................................................i............................ ....i.....................i..................................................... ...................N........N..................N................................ ..............N.................N..N..N.................N.....N................. ................................................................................ ..........................N.........N...............................i........... .....................N.......................................................... ........N.............................N......................................... .........N.. Pos Score Pred ------------------------ 71 0.329 - 78 0.252 - 165 0.154 - 197 0.321 - 312 0.588 Yes 461 0.704 Yes 498 0.429 - 634 0.509 Yes 673 0.435 - 723 0.327 - 790 0.533 Yes 841 0.753 Yes 952 0.357 - 1104 0.403 - 1172 0.721 Yes 1205 0.672 Yes 1227 0.658 Yes 1300 0.270 - 1309 0.337 - 1328 0.327 - 1375 0.290 - 1393 0.278 - 1396 0.142 - 1399 0.389 - 1417 0.284 - 1423 0.147 - 1547 0.201 - 1557 0.186 - 1589 0.594 Yes 1622 0.458 - 1689 0.124 - 1719 0.103 - 1770 0.047 -

Explain the output -- Processing locus 350: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCATCACACTACACCGTTCATTGTCTCTCTCAACTTCAACCACAAAAGGTACTCTCTCTTTCTCACATGGTTAAGGTATA AACTAATAATGATCTTGATGAATGACTTTGGAAGTTAATATATTACTCTTTCATAGAAAATCAAAACAGTTCGTGATGGC TCAGAAGTTAGAAGCAATAGGTAGAGGCCTCCAGTGGGATGATTCATCTGACCATGACAATGTAACAAAGATTCTTGTAC GAGGTGGTCGTGAAGGCATTCAGTACGTCAAGTTTGATTATGTCAAGAGTGGGCAACCCCAAACTGGATTAATCCATGGT CTCTCGGGTAGAGGAGGATTCACACAAACGTTTGAGATTGATCAAAAGGATGAACATCTAGTATCTGTTGAGGGCTACTA TGATGTCACTAAGGGTGTGATCCAAGCACTTAAATTCAAAACCAACAAGAAGACTTCGGAGATGATAGGATACGATGACA CGGGTATTAAGCTTTCACTTGAAGTCAAGGGTAAAAAGATAATTGGCTTCCATGGATATGCCGAGACTAACTTAAACTCT CTTGGAGCTTATTTTACAACGACTGGTCCTATTGGATTGAACCCCCAAGTTGGTCATATTAAATTGGCCTATCAAGGTGG TGGCGGAGGGATCCCTTGGGATCATGGTCCTAATCACAACGGTGTGAAAAGGGTTAGCTTTATCTTTGATGAAAACGAGA TAAGGCAATGGAGGGTCGACTATGACGATGGTGGAGTGATAAGACAATATGAGCCGATCAATGGATATGATATGTTTGAG GTCAAAGAATATCCAACTGAATACATCATATCGGTGGAGTGTACCTACGATGATGTAATACCTAGAAGTGGCAGGCGTAT GATCAGGTCAATAATGTTCAAAACTTCAAAAGGGAGAGTCTCTCCGATATTTGGATACCCTGCTGCTCGCAAATTTGTGC TCGAAAACAACGGTGGTGCTCTTATCGGCTTCCATGGAAGAGTTGGTGCTGGTATAGATGCCCTCGGAGCTTATTTCTCT TCGTTTATTCCTTCTCCGCCTCCTCCTTCCCCCGAAAAACTACAACCAGAAGGTGGTGAGGCAGTAGGAGATCCATGGGA CGATGGTATTTTCAATGGTGTGAGAGAGATACATCTAGAAGACGGTGAGGGTATCGCCCTCAAGTTTGTGTACGATAAGG ACGTCCAGGTGACTGAACTGAAAGTTCATGGGGAACCATCAGGAATAGGATTCAATGAGTTCAAGCTAGATTATCCGAGT GAATACATCACTACGGTGGAAGGTTTTTGGGACAAAACAAGTGGAAATGAACGCGGAGTTATAACCAGGCTTCGGTTCAC GACCAACAAACAAACCTTTCGACCGGTTGGATTGGAGTCGACAACAAGCTTCAGTCTCGGGAAGGAAGGTTACAAGATTG TAGGGTTCCACGGAAATTCTAGTACTGACAAGCTTCACCAGCTTGGGGTCTATGTCGTGCCCATCACGCGCGAGTGATCT TTGCCTAATCTTAAATGAGCTACTTCTCAGATCAATGCTTGATCAGGTTGTCTTATGCTTTTCATGTTTCTGCCATGTTT AATTTTATAAGTTATT ..................................................................N............. ........i........N...i.....................................................i.... ......................................N..............N.....N.................... .......................................N...................................N.... .................................................N.............................N ..N..........................................................i............N..... ...................................................N.....N...................... ................................................................................ .......................N............................................N........... .......N.............N.....N....................N...........N.....N....N........ .................................................N..N.........................N. .............N.................................................................. .................................i.......................N...................... ..........................................................................N..... ..N...........N................................................................. ...........................N..........................i......................... ..............................................N................................. ................................................................................ ...................................................N............................ ..............N...................N...................N........N..........N..... ................ Pos Score Pred ------------------------ 67 0.263 - 89 0.587 Yes 98 0.495 - 102 0.745 Yes 156 0.757 Yes 199 0.306 - 214 0.261 - 220 0.187 - 280 0.209 - 316 0.305 - 370 0.206 - 400 0.093 - 403 0.116 - 462 0.702 Yes 475 0.308 - 532 0.405 - 538 0.248 - 664 0.119 - 709 0.118 - 728 0.485 - 742 0.169 - 748 0.247 - 769 0.341 - 781 0.169 - 787 0.173 - 792 0.405 - 850 0.121 - 853 0.165 - 879 0.363 - 894 0.352 - 994 0.515 Yes 1018 0.486 - 1115 0.386 - 1123 0.206 - 1135 0.455 - 1228 0.217 - 1255 0.544 Yes 1327 0.471 - 1492 0.259 - 1535 0.125 - 1555 0.079 - 1575 0.033 - 1584 0.029 - 1595 0.050 -

Explain the output -- Processing locus 351: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAGCTTCACATAGATTAATAAAGGAATCGACTTTGTTCTGTTTTAGCGACTATGTTTACCTAAGCTGTGTAAACTTAAG AGAAACCCTTTTGCGGATTGAATGTATGATTTATGCATATCTTTCTCTTATGTTTCAGGCCAAAGTATGGAGCTTTCTGA TCCAAACAATGGCAAGACCAACAAACCAAAGCAACCTCCAAAACGTTTCATCAAGAACCAAATCCCTGATTCAATCCTCG GTGATGCATCCCTCAACGCTGCTATCTCCATCTTACCTTCAATTTACCATTTTGAAGTCCATAAATGTGTGTGGCGTATC AAAACATCAAACGCTAAACGCATAGCTCTGCAGCTTCCAGAGGGTCTTCTTATGTACGCTCTTACACTCTCTGATATCTT CACATCTTTTGCTGGAGCTTCTCACTGTTTTGTACTCGGTGATGTTACTTATGGAGCTTGTTGTGTTGATGATTTCTCTG CTTGTGCGCTTGGTGCTGACTTGCTTATTCACTATGGACATAGTTGTCTTGTCCCTATCGACTCTACAAAAATCCCTTGC CTTTATGTTTTTGTTGAGATCCAGATAGATGTCAAATGCTTGTTGAACACTATACATCTGAATCTCGCGAGTGATGTTAA GAACATCATTCTTGCAGGCACGATTCAGTTCACTTCGGCGATTAGAGCTGTGAAACCAGAGTTGGAGAAGCAAGGGTTCA ATGTTTTGATCCCTCAGTCAAAGCCTCTATCTGCTGGTGAAGTCCTTGGCTGTACAGCTCCCAAGGTGAAGACGGTTGAG GATTGTAAAGACCAAGTACTGGTCTTTGTAGCTGATGGGAGATTTCATCTAGAAGCATTCATGATAGCCAATCCAAAAAT CAAGGCGTTTAGGTATGATCCATATCTTGGCAAGCTGTTTTTAGAGGAGTATGATCATAAGGGAATGAGGGAGACGAGAA TGAGAGCGATTGCACGGGCCAGGGAGGCTAAAACTTGGGGGATCGTGTTGGGGACATTGGGAAGGCAAGGAAATCCTAAG ATTCTTGAGAGATTAGAGAAGAAAATGATGGAGAAAGGGATAGATAGCACAGTTGTTTTAATGTCGGAACTTAGTCCCAC TAGAGTGGCATTGTTTGAAGACTCGGTGGATGCTTGGGTCCAAATAGCATGCCCAAGACTTTCTATTGATTGGGGTGAAG CGTTTCTTAAGCCGCTTCTGACGACTTTTGAGGCTGAGATTGCTCTAGGATTCATCCGCGGCTGGTGGGAGAATGATTCT TCTAGTAGAGTAAATTCTTCATCTGGCTGTTGCAAAGAGGACAAAGAAACAAGCTGTGCGTGTAAAAATGAGAACGTGAA AGATGACAAGAAAGACAATGATGGGGCACTCGATGGAGACTATCCAATGGATTACTATGCACAAGAAGGTGGAGAATGGA ACTCTTCATATCTCAAGAAGTCATCTCGTCCCATTCGAAGAAACCCTTTACCTTCTTCTGTAGTTTAGAATGCTGACAGA GTAAATTCAACCACAGCAGGTATTACCAAGTCAAAAAGGCAAATGGGTTTTGATGATGTTCCTGGTATTTTCGGATGGGT TGATTTG ....................................................N........................... .....................i...N......N................N................i............. ........i....................................................................... ...N............................................................i............... ...................................................N............................ .........................................N........i.................N........... .................................N.............................................. ....N.......................N......i.....................................N...... ................................................................................ N............................................................................... ..................................N.........................i................... ..............N...................................N.............N..............N ................................................................................ ........................i..i................................i................... .............................N..................i............................... ........................................................................N....... ...................................................................i............ ..i..............N..N...........N.............N.........N..................N.... .....................................................................N.......... ..........................................N.........N..N..................N..... ....... Pos Score Pred ------------------------ 53 0.198 - 102 0.701 Yes 106 0.290 - 113 0.170 - 130 0.200 - 147 0.795 Yes 169 0.534 Yes 244 0.353 - 305 0.728 Yes 372 0.361 - 442 0.157 - 451 0.576 Yes 469 0.094 - 514 0.121 - 565 0.104 - 589 0.331 - 596 0.830 Yes 634 0.129 - 721 0.212 - 835 0.153 - 861 0.764 Yes 895 0.198 - 931 0.166 - 945 0.292 - 960 0.248 - 1065 0.575 Yes 1068 0.619 Yes 1101 0.534 Yes 1150 0.151 - 1169 0.522 Yes 1273 0.375 - 1348 0.601 Yes 1363 0.760 Yes 1378 0.423 - 1381 0.434 - 1393 0.253 - 1407 0.419 - 1417 0.301 - 1436 0.273 - 1510 0.236 - 1563 0.132 - 1573 0.060 - 1576 0.047 - 1595 0.077 -

Explain the output -- Processing locus 352: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCATTGCAGTTCTGATTCACTCTCTTGCTTTTTAATTCCTTTTTTGGTGATTCTTGTCTTATTTTGTTACTGTGTTCTTG ATACTGTTGTCTCCGTACTAAGCTCTTTTTGTAATGGATCTGCAGATGGAGACTGTGAGACCAATGGTTGTTGCTCCTAA GGGTAACAAACTCAGACGCAAGTTTGCTAAAGTTCTTAACATTCATAAGCTAACGGGTGTTGCTCCAGAAGGAGAAATGA AGAAGATAAAGTTCGATTCCAAAACCGCTAAGTTGTCTGAGTCGTTCTACAAGCTTGAGGAAGAGTATGAGAGAAGTCAG GGATTGGAAGCTCTTCTTGCGAAGCTTTTTGCTACGGTTTCTTCGATCAAAGCGGCTTATGCGCAGTTACAGCATTCTCA GTCTCCGTATGACTCTATCGGGATTCAAAAAGCAGATAACTTGGTAGTCGCCGAGCTTAAGACTTTGTCTGAGCTGAAAC AATGTTTCATGAAGAAACAAGTGGATCCAAATCCCGAAAGAACTCTGGTTCTCGCGGAGATTCAAGAGCTGAGGAGTCTA TTGAAAACGTATGAGATAATGGGGAAGAAGCTGGAGTCTCAGTACAAGCTTAAAGACTCAGAGATTATATTTCTGAGAGA GAAACTTGATGAATCGATGAAACAGAACAAGTTGACAGAGAAGAGACTTAACCAAAGCGGACAACTTTGCAATCCACTTG ACAATCTTCACCTATCTGCGCTAAATCCAACACATTTCGTTACTTATCTGCACCACACAGTCAAGTCCACCAGAGGTTTC GTCAAGCTAATGATCGAACAAATGAAACTCGCTGGATGGGATATTTCATCAGCTGCTAATTCGATCCATCCCGGTGTGTT CTACTACAAGCAAGACCACAAGTGTTTCACTTTCGAACACTTTGTCTCCAATGTGATGTTCGAAGCGTTTCATCTACCGT ACTTCTCAACTTCAAGCGAGTCAAGAAGCTACAAGAAGAAGAAACAGAGCAATGCAGATAGAGAAATGTTCTTTGAAAGG TTTAAAGAACTCAGGTCGATGAAAGCGAAAGATTATCTAACCGCGAGGCCTAAATCAAGATTCGCTAGGTTCTGTCGAGC CAAGTACTTGCAGCTTATACATCCAAAGATGGAGCAAGCTTTCTTCGGACATTTGCATTTGAGAAACCAAGTTTCTGCTG GTGAATTCCCGGAGACGAGCTTATTCTCTGGATTTCTTGAAATGGCGAAACGAATTTGGCTTTTGCATTGCTTGGCTCTC TCTTTTGAACGTGAAGCTGAGATCTTTCGAGTACCTAAAGGTTGTAGATTCTCTGAAGTGTATATGAAGAGTGTAGCAGA AGAAGCGTTTTTCCCGGCGGCGGAGAGTTCGCCGGAGTCTGAGCCACGTGTCGCGTTCACGGTTGTTCCAGGGTTTAGAA TTGGGAAGACTTCAATACAATGCGAGGTCTATCTCTCGCTTTCATGATCGCCGTTTGATCTAAGATCCGAAAACGGACGG TGAAGATTCTTGGGCGGTTTGGTAATTTCTGAAAATGGTGGGGTATGTTTAGAAAATTGAGAAAAA ................................................................................ .................................N...........i.................i................ ............................................................................i... ..................................................................N............. ..........................................................N..................... ........N....................................................................... ..........N.......N............................................................. ........N.......N............................................................... ................................................................................ .........N...........i.............N............................................ ..................................................N....i........................ ...................................................N.............i.............. ..................N............................................................. ............................i................................................... .........................................i...................................... ...............................................................N................ ................................................................................ ...................i.......................N.................................... ..................................N.........N..................... Pos Score Pred ------------------------ 114 0.488 - 126 0.825 Yes 144 0.807 Yes 237 0.679 Yes 307 0.334 - 379 0.115 - 409 0.104 - 482 0.838 Yes 489 0.349 - 571 0.292 - 579 0.495 - 649 0.195 - 657 0.174 - 810 0.491 - 822 0.640 Yes 836 0.090 - 931 0.212 - 936 0.589 Yes 1012 0.490 - 1026 0.532 Yes 1059 0.315 - 1149 0.665 Yes 1242 0.830 Yes 1344 0.492 - 1460 0.551 Yes 1484 0.374 - 1555 0.162 - 1565 0.057 -

Explain the output -- Processing locus 353: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 354: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 355: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAAACTTGTAGGTTAGAAAGGAGATGAAATGAGAAAGTTAGGGGCTTTTCTACAATTGTCTTCGTTTATGATCACGAA TTGAATAGTTGACAAGTCTAAAATTTGTCTCTTTCCTTGTCACTTGTCAGTGAGTATACAAAACAATACACAGAGCCTCG CATGTCATATTCACACAGGGACCTTCCGTTAAACTCCTTTTTTATAATCTCGTTCTCTCTTCTCTCTTTCTCTCTCTGGT TGCTCTTGGCCATGGTTGTGAATAGTCAAGCTTTCTTGTTGGCATTAATTGCATTACTTGCTACCCAATTGCCTTCACTA ATGGCTGCAGATTGTCCATTAGATTTCAGTGGATCAAATTTCACTCTAGTAGCTACTGTGTGCTCCAACATAACCAACAG AGGCAAATGTTGCCGCTACATGAACGCTTTTGTTGCGGTGTCCGTGGCTCGTTACGCGAACTTGTCAACTAATCTTGGAG TCACATCAGATTTATCTGAAACCTGCATTGCTTCCATCTCTAGAGCCATGGAGGGCTACGGAGTCTCGAGAAACGCCACT AGTTTCTGCGGTTTGGGAACCAAGATCCTTGTTAAGTATGACTGTGACGGTCGAACCACTGTTACACAAATGCATCAGTC TCCGGGATTTGGACATGTTTCAAGAAACTGCAGACTCCCATTTTCGCCGGGACACCAATGCAGAAAGTGTTTGAACTCTG GCATCACTTACCTTCGTAATCTCATAGGTGCAGAGACTAATAACATTACTCTGTGTACTTGTCGTGATGCGACTTATGCT ACATTAGCAAGCCGAATAGACGATACATCAGCTCTTGAACTCCTTAGTTGTTTCTTTCAAGTGACAGAGCTTAACATTCC TTCAGAGTCATTTTCACCAGTGGCAAGTCCAGAACCTTCTCCAAGCACGGTTGGTGGTATTAGCCCAAGCAACAGTGATT CTCAAATGACTACAAGTAGAAGCACCAATCCATATCACTTAACAATGGTTCCAACCATTGGGATTGTTGTTACAGCTGTT GCTCTTACGATGCTTGTAGTCTTGGTAATTCTTATCCGTAGAAAGAACCGGGAACTGGATGAATCTGAGAGCTTGGATAG AAAGTCAACAAAATCAGTTCCTTCTTCCCTTCCTGTATTCAAGATTCATGAAGATGATTCTTCCTCAGCTTTCAGAAAGT TCAGCTACAAGGAAATGACAAATGCAACAAATGATTTCAACACGGTTATCGGTCAGGGAGGTTTTGGTACTGTTTACAAA GCCGAGTTCAACGATGGTCTGATTGCAGCGGTAAAAAAGATGAACAAAGTCTCTGAACAAGCTGAACAAGATTTTTGTAG AGAGATAGGGCTTTTAGCTAAGCTGCATCACCGTAACCTCGTTGCTTTGAAAGGCTTTTGCATCAACAAGAAAGAAAGGT TCTTGGTCTACGACTATATGAAAAATGGCAGCTTAAAAGATCACTTACATGCCATTGGAAAGCCTCCACCTAGTTGGGGA ACAAGAATGAAAATTGCAATTGATGTGGCAAATGCTTTGGTAAATATTCCAAAGTCTTTCATCTACATTTTCTCTGTAAA CTGATTTGATAATATATCTTTTACAGTTTTTGGTTCTTGGCTGCACGTTGTTCTCTCCTCCAGGAATATCTTCATTTCTA CTGTGATCCTCCTCTCTGTCATA .........................N....N......................................N.......... ................................................................................ ...........i.................................................................... i............................................................................... ......i............i............................................................ ...............................................i................................ .....................................N...............................N.......... ..............i..........................................N...................... ..................................................................N........N.... ................................................................................ ................................................................................ .....i......................................i................................... .........N................................................N..................... ...............................................N.....N.......................... ..............i......N........N................................................. .............N.........................N........................................ ................................................................................ .................N......N.......................N............................... ......i...............N........N................................................ ................................................................................ ....................... Pos Score Pred ------------------------ 26 0.116 - 31 0.442 - 70 0.179 - 162 0.217 - 252 0.707 Yes 321 0.644 Yes 407 0.510 Yes 420 0.533 Yes 528 0.831 Yes 598 0.488 - 630 0.249 - 655 0.663 Yes 698 0.128 - 787 0.074 - 796 0.115 - 966 0.787 Yes 1005 0.863 Yes 1050 0.308 - 1099 0.141 - 1168 0.309 - 1174 0.376 - 1215 0.889 Yes 1222 0.263 - 1231 0.357 - 1294 0.249 - 1320 0.409 - 1458 0.370 - 1465 0.420 - 1489 0.145 - 1527 0.510 Yes 1543 0.083 - 1552 0.088 -

Explain the output -- Processing locus 356: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAAACCTAGGACCGCTTCCCTTGCCTTGTAAGTTAATGTTTTTTATTTAAATAATCAAGTCCATGTTTTATTGACTCTG TGGATATGAATATATACCTAAACATGCAACCAAATGAAGAGGAATCTGTTCTCATCCATAAACCAACGAAAAATGAAGGA CCAACTTGGATTCAGAATTGTGCTCTGTTCTGCGGTTTTCATAATTCTGACTCAAAACAGAGCTCTCGCAGACTTGGACA GCGAGTCCCATGAGGTTAACTCAGAATCGGTAGGAGAAGAACAATGGGAGATGGTGCAGAGGAAAGGGATGCAATTCACT CTAAATGGACAACCATTCTATGTGAATGGCTTCAACACATACTGGATGATGACTCTTGCAGCTGACAATTCCACGAGAGG CAAAGTGACGGAAGTGTTCCAACAAGCCTCTGCGGTTGGAATGACGGTAGGCAGGACATGGGCTTTCAACGATGGCCAAT GGAGAGCTCTCCAGAAATCTCCCTCTGTTTACGATGAAGAGGTCTTCAAGGCCCTCGACTTCGTGCTGAGCGAGGCCAGA AAGTACAAAATCAGGCTTATACTGTCATTGGTCAACAACTGGGACGCCTATGGCGGCAAAGCTCAGTACGTTAAATGGGG AAACGCCTCCGGCCTTAATCTCACTTCCGATGACGACTTCTTCACCAACCCCACCCTCAGAAACTTCTACCAATCCCATG TCCGGACAGTGCTGAATAGAGTGAACACCTTCACAAACATAACTTACAAGAACGACCCCACAATCTTCGCCTGGGAACTT ATGAATGAGCCTCGTTGCCCCTCTGATCCTTCCGGCGACAAACTCCAGTCTTGGATACAAGAAATGGCGGTGTTTGTGAA GAGCTTAGACGCTAAACACTTGGTGGAGATCGGACTTGAAGGCTTCTATGGTCCCTCTGCCCCTGCGAGAACCAGATTCA ACCCCAACCCCTACGCTGCACAAGTCGGAACTGACTTCATCAGAAATAATCAGGTTCTTGGCATTGATTTTGCCTCGGTT CACGTCTATCCAGATTCCTGGATATCTCCGGCAGTATCAAATTCTTTCCTGGAATTCACAAGCTCTTGGATGCAAGCTCA TGTTGAGGACGCAGAGATGTACTTGGGAATGCCGGTTCTGTTCACAGAGTTTGGTGTGTCTGCACATGACCCTGGTTTCA ACACGTCTTTCCGTGACATGATGTTGAACACAGTGTACAAGATGACCCTCAACTCAACAAGGAAAGGAGGGGCTGGTGCC GGGAGTCTGGTCTGGCAGGTCTTCCCTCAGGGGGCAGAGTTCATGGATGACGGCTATGCGGTTTACCTCACAAGAGCTCA CACAGCTTCAAAAATCATATCTCTGCAGTCAAAGCGACTAGCCATCTTCAACTCCTTGTGTAGCTGGAGATGCAGATGGG GATGCAAGAAAAAGAATCAGACTGCTCTTGATGCCCTCCTCTCGCACGACGAGCTTTAAGTATCACAATCTTTTGTGAAT TACAACTGCGTGAATACAAAGATCAGTGTATACAAGTTTTTAACAAATATTATATATGTAAAGAATCAAGATGATTGT ....................................N..........................N................ .....N.................i.........N......................................N....... ................................................................................ .........N.................................N......i.................i........... ....N..............N.....N...................i..i............................... ........................................i................i.............i......i. .................................i.............................................. .................................................i........................i..... .............................N...............................................N.. ................................................................................ N...i..........................................................i................ ...............................................N................................ ................................................................................ .....................................................................i.........N ................i...........i....................................N.............. .................i..i....................i...................................... ..........................................N...N........N........................ .....................................................................N.....N.... .......................................................N..............N....... Pos Score Pred ------------------------ 37 0.202 - 64 0.175 - 86 0.396 - 104 0.568 Yes 114 0.423 - 153 0.499 - 250 0.233 - 284 0.419 - 291 0.510 Yes 309 0.604 Yes 325 0.445 - 340 0.318 - 346 0.498 - 366 0.607 Yes 369 0.727 Yes 441 0.630 Yes 458 0.707 Yes 472 0.518 Yes 479 0.507 Yes 514 0.552 Yes 610 0.717 Yes 635 0.676 Yes 670 0.350 - 718 0.430 - 801 0.434 - 805 0.621 Yes 864 0.890 Yes 928 0.089 - 1110 0.615 Yes 1120 0.271 - 1137 0.686 Yes 1149 0.526 Yes 1186 0.343 - 1218 0.581 Yes 1221 0.587 Yes 1242 0.603 Yes 1323 0.303 - 1327 0.423 - 1336 0.293 - 1430 0.145 - 1436 0.360 - 1442 0.254 - 1471 0.123 - 1576 0.087 - 1591 0.067 -

Explain the output -- Processing locus 357: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCTGATGTGTGGAGCCTTAAAGTCTCTGACTTTTGTTTCATTAATAAGCCGGATAGATAGTAAATGCTGAAGAAGGTTT AGTGGTTGTTAAAGAGCGTAAATGATCTTAAAGTGCTTACCTTTTTGTTTTCTTTTTTGTCTTTTGGCTGTGTTAGGAAT GGCATCAAGCTCTTGTCTCAAGCAAGGGTCAGTGCCAATGAACAATGTTTGTGTTACCCCTGAAGCAACGTATGAGGCTG TGGTTGCAGACCCTAGGCTTTTCATGACTTCCTTGGAAAGGCTGCACTCTCTCTTGGGTACTAAATTCATGGTTCCGATC ATAGGAGGAAGAGATTTGGACTTGCACAAGCTTTTCGTTGAGGTAACCTCACGTGGCGGAATCAATAAGATACTTAATGA AAGGAGATGGAAAGAAGTAACTGCTACATTTGTCTTTCCTCCAACTGCAACAAACGCATCATATGTTCTGCGCAAATACT ACTTCTCGCTGCTGAACAATTATGAGCAAATCTATTTCTTCAGATCTAATGGTCAGATTCCTCCAGACTCTATGCAGAGT CCATCTGCTAGACCATGCTTTATTCAGGGAGCCATTAGACCTTCACAAGAACTTCAAGCTCTAACCTTTACCCCACAGCC CAAGATCAATACTGCAGAATTCCTCGGAGGATCACTTGCTGGATCAAATGTGGTTGGAGTGATCGATGGAAAATTCGAAA GTGGTTACCTTGTAACTGTAACAATTGGATCAGAGCAGCTTAAAGGAGTTCTGTATCAGCTTCTTCCTCAGAACACAGTT TCTTATCAAACTCCGCAACAGAGTCATGGTGTCCTCCCGAACACTCTGAACATTTCTGCCAATCCTCAGGGGGTTGCAGG AGGAGTAACAAAACGCCGTAGGAGAAGAAAGAAATCAGAGATCAAAAGACGTGATCCTGATCATCCAAAACCCAACAGAA GTGGTTATAACTTCTTTTTCGCAGAGCAGCACGCGAGACTTAAACCGCTTCATCCTGGTAAGGATAGGGATATCAGCAGG ATGATTGGTGAGCTATGGAACAAGCTGAATGAAGACGAAAAATTGATTTACCAAGGGAAGGCAATGGAGGACAAAGAGCG ATACAGAACTGAAATGGAAGATTACAGAGAGAAAAAAAAGAATGGACAGCTCATAAGCAACGCTGTTCCGCTGCAACAGA GACTTCCGGAGCAAAACGTAGACATGGCTGAAGCTGACCTTCCAATTGATGAAGTCGAAGAAGATGACGAAGAAGGTGAT TCAAGCGGATCCTCAGGCGAAAGCGAGCCACATGATGATCAGAGCATAGAAACTGATCCTGAATTGGAAGAGCCATCTCT GAATCCCTCAGGTCCAAACCTAAACCCTAATCCGACTGAGATAGTGGTGGCTCCTAAGGAGAAAAACGGCGATGTTGTGA TGGAAACATCTCCATTGAAGAAAGCCGATGAGCCAACTGTGGCGGTGACTGCTGAGCAGAACTGAAGATTCTTCTCTTGT TTCTTACTCCCCGTTGCCAGTTTCGTGTTCCATAGGTAAATGTTAGAGAGAGGAAAGGGCTTCAGATACCTTGCTTCTGG CCCA .....N..........................................................N............... .....................N........................................................i. .....................................i......i..........................N........ .......................N............................................N........... ............................................................................N... ......i.......................................................i................. .....................N..........................N......................i........ ..............i................................................................. ...............................................N.................N.............. ................................................................................ .........................i...................................................... ................................................................................ ................................................................................ i.............N.............N..................................i................ .............i...........................N...................................... .......................i........................N..............N................ ...............................N..N............................................. .......................................................................N.......i ...........................N.................................................... .......................................N........................................ .... Pos Score Pred ------------------------ 6 0.367 - 65 0.268 - 102 0.243 - 159 0.635 Yes 198 0.516 Yes 205 0.626 Yes 232 0.180 - 264 0.252 - 309 0.395 - 397 0.345 - 407 0.530 Yes 463 0.759 Yes 502 0.466 - 529 0.393 - 552 0.665 Yes 575 0.615 Yes 688 0.410 - 706 0.182 - 826 0.775 Yes 1041 0.679 Yes 1055 0.448 - 1069 0.496 - 1104 0.528 Yes 1134 0.655 Yes 1162 0.423 - 1224 0.892 Yes 1249 0.254 - 1264 0.267 - 1312 0.218 - 1315 0.161 - 1432 0.280 - 1440 0.509 Yes 1468 0.167 - 1560 0.076 -

Explain the output -- Processing locus 358: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCGACTTTGACGTCTTTGAGCATCCATCCATGTTGAAGAATCCCATTATTCGTATGCTTTTCTTCTATGGCCTTGCATCA GTCAGCACTCAACTTGTTTGAATGTGCAGGAGCGTGTTTCCAGAGGGGATGGATTCTGTTCGACGCACTTACACCATTAT GCGAGGTCGTCACATAGACAAGCGTGTTCCTTCTAAAGAGTTTCTCTCTGCTTTGGATAAAGCTGAAACTGCAGTACGTC TTCCTACTGGGACGGGAAAAGACTGCGGGAATCCTTCCTGGAAGCGATCTCTGCCTCACGTGCTCGTTGCTTCTCTGACT TCGCTTTTATTCGGCTATCATCTCGGGGTTGTTAATGAAACTCTCGAAAGCATATCAATTGACCTTGGCTTTAGTGGGAA CACCATAGCTGAAGGTCTTGTGGTTAGCACCTGCTTAGGCGGCGCCTTTATTGGGTCTCTGTTCAGTGGGTTGGTAGCTG ATGGTGTTGGGCGCCGTAGAGCTTTCCAGTTGAGCGCTTTACCAATGATTGTTGGAGCTTCTGTGAGTGCATCAACTGAG AGTCTTATGGGCATGCTCCTGGGAAGGTTTTTGGTCGGAATTGGGATGGGCATAGGTCCTTCTGTTACAGCTCTCTATGT CACTGAGGTGTCACCTGCTTATGTGAGAGGTACATATGGGAGTTCTACCCAAATCGCAACATGTATTGGACTCTTAGGTT CGCTGTTTGCTGGAATCCCGGCAAAAGATAACTTGGGCTGGTGGCGCATATGTTTTTGGATATCCACAGTCCCAGCTGCA ATGCTTGCTGTCTTCATGGAACTCTGCGTGGAAAGTCCTCAGTGGCTTTTTAAGAGGGGAAGGGCAGCTGAAGCTGAAGC TGTGTTTGAGAAGCTATTGGGAGGGTCGTACGTCAAAGCTGCTATGGCAGAGTTAGTGAAGTCGGATAGGGGAGATGACG CCGATTCAGCCAAATTGTCGGAGTTGCTTTTTGGACGCAGTTTTAGAGGATCAACTGTTGCTGTGGTTTTGATGGATAAG CTGGGGAGAAAAGTGTTGCTTATTGGGAGTTTTGCGGGCATGGCAGTATCGCTGGGTCTCCAAGCAATAGCATATACTTC GCTTCCTTCGCCCTTTGGAACCTTGTTTCTCTCGGTTGGAGGAATGCTGCTGTTTGTGTTGTCGTTTGCAACTGGAGCGG GTCCTGTTCCAAGTCTTCTCCTGTCTGAGATATGCCCTGGTCGACTTCGAGCAACTGCGTTGGCTGTTTGTCTTGCCGTT CACTGGGTTATAAACTTCTTTGTCGGGCTGCTGTTTCTGAGAATGCTGGAGCAACTAGGTTCGGTGCTTCTAAATGCAAT ATTTGGGTTCTTCTGTGTGGTGGCAGTCATCTTTGTGCAGAAGAACGTGGTTGAGACTAAAGGAAAGAGCCTACAAGAGA TAGAAATCAGTCTGCTCTCTTCCACCCAGTGATTGATCTCTTTATTCTGAGAATTCGTATTATTCTTCTGGCTCTCTTTT TTTCCTTGGAGAGACACAAGTAACGTGTACCTAATATGGAAGTAGGAGTGA .............................N.......................N............i............. .....................i..........................i.............................N. ................................................................................ ................................................................................ ..................................N............................................. ................................................................................ N...........................................N................................... ......i.....i................................i..............................N... ....................N..............N........................i................... .................................................N.............................. i..............i................................................................ ...........................................N..............................N..... .......................................................................N........ .......................................N........................................ ...........................................i.................................... ...............................N................................................ ..........................................i..............................i...... ................................................................................ ................................................................................ ...................................N............... Pos Score Pred ------------------------ 30 0.456 - 54 0.236 - 67 0.586 Yes 102 0.530 Yes 129 0.688 Yes 159 0.495 - 355 0.230 - 481 0.232 - 525 0.384 - 567 0.637 Yes 573 0.563 Yes 606 0.601 Yes 637 0.295 - 661 0.247 - 676 0.334 - 701 0.760 Yes 770 0.444 - 801 0.592 Yes 816 0.502 Yes 924 0.491 - 955 0.225 - 1032 0.476 - 1080 0.136 - 1164 0.547 Yes 1232 0.384 - 1323 0.511 Yes 1354 0.534 Yes 1556 0.067 -

Explain the output -- Processing locus 359: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTCTTCTTTTATATGATTCTTCTCCAAAACCAAACCACTAGATAATGTGAAAAAAGTGACCACCTGCTCCATTTCATC AACTCCATCTTCTTGTATTAACTATCTCATTTCATGTATGTATATATACTCTTTGAGAATTTGATTCTATCATCACAATC ATTTACTTCAAACAACAATCTTTGCTTCTCTCTGAGTGATCATCTTTCTTGTTCAATGGCTGGTCAAAAGATACTTTCTT TACTGGTTATAGCTCTTGTTGTTACTTTTGCAGCTGCGGCTCGGCTTCTTGATGAAGAAAATGCATTCTCAGCTACCACA ACCACCCTTGGCTCAGGTTCAGGTTCCACCGGAATTGGTTTTGGTGCAGGAACAGGGTCAAGTGGTTCAGGTTCCACGGG ATTCGGTTTTGGTGCAGGATCAGGGTCATCTGGTTCAGGTTCTACTGGAAGCGGTCTCGGTGCAGGTACAGGGTCAATAC CATCAAGTGGTTCAGGTCCTGGTCTTCTACCAACTGCCAGTTCTGTTCCTGGTTCTTTAGCGGGTGGTGGTTCAGGTTCC TTGCCTACCACTGGTTCAGCCACTGGTGCAGGTGCAGGTACCGGTTCAGCTCTTGGAGGTGGTCCAGGTGCTGGCTCAGC TCTTGGTGGTGGTGCGGGTGCCGGTCCAGCTCTTGGTGGTGGTGCGGGTGCCGGTCCAGCTCTTGGTGGTGGTGCAGGTG CTGGTTCAGCTCTTGGTGGTGGTGGTGCAGGTGCTGGTCCAGCTCTTGGTGGTGGTGGTGCAGGTGCTGGTCCAGCTCTT GGTGGTGGTGTTGCAGGTTCTGGTTCAGCTCTTGGTGGTGGTGCAAGTGCTGGTCCTGACAATACGTTGGTATTTTTTAT GCATGATATACTCGGTGGTTCAAATCCCACTGCCAGAGCCGTAACTGGAGTCGTCGCAAATCCGGCTTTAAGCGGGCAAC TTCCATTTGCTAAACCCAACGGCGCAAACCTTCCTGTAAGCAACGGAGTTCCGTCAAACAACAACAACAATGGGATTGTC AACAACAACAATGTCCCTTTCCTCGTTGGACTCGGCGGAACCACAGCTAACATTCTCCAGAACAACAATAACGGAAACAA CATTTTAAATGGTTTCCCTGTTGCTAGTGGCGGTCAGCTCCCGAGCGGTTCTGCTCTTCAGATGCTAATGTTCGGGACAA TGACAGTAATCGATGATGAACTAACTGAAGGCCATGAGCTCGGATCTGGCTTACTCGGGAAAGCGCAGGGATATTATGTG GCGAGTGCAATTGACGGAACTAGCCAGACAATGGCTTTCACCGCGATGTTTGAAAGCGGCGGTTATGAAGATAGTATAAG CTTCTTTGGGGTTCTCAGAACAGCGGTATCAGAATCGCATATAGGAGTAATGGGAGGTACCGGAAAGTATGTGAATGCGA GGGGATTCGCGATTCTGAAGACGTTTACAGGATCTAGCGGGACGCAGCAGAATCAGCCGCATCAGTTCACTGATGGGCTT GAGACTGTTGTTGAGTGTACCGTTTATCTTTCTTACTAGTTTTATTTTTCTTCTTTTGTTGGGGTTTGGGAATGTTTGGT GTGTATGTTATGTGTGTCATTTGTAATCTAAATATGAAATTTGTGTAAGAAAATTGTA ..............N...............................N................................. .................................N...N.......................................... .......................................................i........................ ...................................................N........i................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ..............................................................................i. ..N............................................................................. .....................................................................i.......... ..........N..................................................................... ........N....................................................i.....i...........i ............N..N.................N.........................................N.... ..............................N..............N..................N............... .................................................N..................N.....N..... ........................................................................N....... .......................................................................N........ ....N....N.......................N........................ Pos Score Pred ------------------------ 15 0.191 - 47 0.378 - 114 0.331 - 118 0.185 - 216 0.866 Yes 292 0.192 - 301 0.687 Yes 879 0.837 Yes 883 0.217 - 1030 0.606 Yes 1051 0.328 - 1129 0.308 - 1182 0.610 Yes 1188 0.519 Yes 1200 0.719 Yes 1213 0.469 - 1216 0.393 - 1234 0.140 - 1276 0.148 - 1311 0.331 - 1326 0.338 - 1345 0.377 - 1410 0.486 - 1429 0.157 - 1435 0.238 - 1513 0.189 - 1592 0.089 - 1605 0.052 - 1610 0.068 - 1634 0.043 -

Explain the output -- Processing locus 360: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATATATTATATAAATCACCTTAGAAACGTTATATAAGCATATTGTTTTAACATTGTTGTCATCCTACAACAAAAGAAA AAAAATATATCTAAAGGATAAGGTAATGGTTTTAATGGTATGACAATGGAAGTTAAACTATATACACTTATTTCAGTTCA TAGAGAATCAAATCAGTTTGCGATGGCCCAGAAGTTGGAAGCAAAAGGCGGGAAAGGAGGCAATCAGTGGGATGATTTAC TTGACCATGACAATATCGCAAAGATTCATGTACAAGGTGGTCATGAAGGGATTCAGTACGTCAAGTTTGATTATGTTAAG TTTGATAATCTCAAGATTGGTCAACCCAAACTTGGATCAATCCATGGTCTCTCGCGTAAAGGATTCACGCAAACGTTTGA GATTGATCCAACCAATGAACATCTTGTATCCGTTGAGGGTTACTATGATGAATCTAAGGGTTTGGTTCAAGGGCTTAAAT TCAAAACCAACAAGAAGACTTCTGACATGATAGGATACGATGAAAATGGTTTGAAGTTTTCACTTGAAGTTAACGGAAAA AAGATAATTGGCTTCCATGGATATGCTCAGACTTATCTAAACTCTCTTGGAGCTTATTTCGTAACGGCTCCTCCTACTAA ATTTGACTACCAAGGTGGTTCAGGAGCCCAACTTTGGGATGACGGTACTAACTACAATGGTGTGAGAAAGATTTCCTTTG CGTTAGATGCCAATGAGATAAGGCAAATCAGGATTGATTATGACAAAGGTGGCTTAATTGAAAGACGAGAGTACGGGGGT AACGTAGGACGACAAGAGGAGTTTGTGGTCGACTATCCAAGTGAATATATCATATATATGGAGGGTACATGCGATATTGT AAGCGATGCTAGTAAAAACCGTGTGAGGTCGCTGATGTTCAAAACGTCAAAAGGGAGAACCTCTCCGATATTTGGAAAGG TTGCTGCTCGTAAATTCGTGTTTGAAAGCAATGGTAGTGCTCTTATCGGGTTTCATGGGCGGGCTGCTGCTGCAGTAGAT GCCATCGGAGCTTATTTCTCCCGGTTTATTCTTCCTCCTTCCGCTGAAACACTACAAGCAAAAGGAGGTGAAGGAGGAGA TCCATGGAGCGATGGTGTTTTCAACGGTGTGAGGAATATATATGTAGGACAAGGTGAAAATGGTGTCAGTGCCGTCAAGT TTGTGTATGATAAAGACTCTCAGGTGGCTGAAGGGAATGATCATGGGAAACCAACACTTCTTGGATACGAGGAGTTCAAG CTTGAATATCCGAGTGAATACATCACCACGGTGGAAGGTTGTTTTGACAAAATATTTGGAAGTGGAGGCGGCGTTATAAC CATGCTTAAGTTCAAGACCAACAAACGAACCTCTCCGCCGTTTGGACTCGAAACAACATCAAACTTTGTTCTCGGGAAGG AAGGTTACAAAATTGTAGGGTTCCACGGAACATCTAGTCATGAGCTTCATCAGCTCGGGGTATATGTCATGCCCATCTAG TGATATTTGCCCAATCTTAAGTGAGCTACAAGTTTTCTTATAAAGTATTGTCTGCTAGTCCTATGTTAATTTGTATTTCG TTCATTTCTGCAATGTCAT ................................................................................ .........................N........N....i.....i.................................. ......................i................................................N........ ......N....................N..............N.............................N....... ...........................................N.................................... ..............N.............................N..N................................ ..........................i............N.....N.................................. ................N.....N......................................................... ......................................N.................i....................... ......N.....N..........................N........................................ .........................................................i..........N........... .....N............................N............................................. ..............................N.......................i.......................N. ................................................................................ ...N.......N.............................N.................N.................... ......N.............................N.....N..................................... ................................................................................ .......................................N.......................N....N........... ..............................................................N................. ............N...... Pos Score Pred ------------------------ 106 0.430 - 115 0.332 - 120 0.718 Yes 126 0.887 Yes 183 0.697 Yes 232 0.114 - 247 0.276 - 268 0.102 - 283 0.202 - 313 0.237 - 364 0.160 - 415 0.136 - 445 0.147 - 448 0.122 - 507 0.679 Yes 520 0.353 - 526 0.241 - 577 0.490 - 583 0.243 - 679 0.214 - 697 0.700 Yes 727 0.287 - 733 0.381 - 760 0.168 - 858 0.535 Yes 869 0.318 - 886 0.144 - 915 0.440 - 991 0.321 - 1015 0.509 Yes 1039 0.384 - 1124 0.072 - 1132 0.181 - 1162 0.224 - 1180 0.347 - 1207 0.248 - 1237 0.094 - 1243 0.381 - 1362 0.267 - 1480 0.344 - 1504 0.240 - 1509 0.159 - 1583 0.069 - 1613 0.040 -

Explain the output -- Processing locus 361: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAATCCCACCAAAATCGATTTAACACTAGTTTTGGTTTATTGGACCAAACCAAGTAGAAAACCAACAAAACCACCAGTAC CGTGATTATTTTCTTTTCTAACGCGTAATAAATATCTTTTATGGCTTTATTATGCACTCTCATTGCGAAAAAAGAAAAAT CCAAAGGCTTGGAAAATCGACAAAAACGAAAAGGGTAGTGGTGAGATGTTTCATCATTCTCTGTTTCAGTTCGGTAATGG CGGAGATTTGCTATTTTGGGGGTCTAGTCCATTATAAATCACCATCTCTTCCTTTGTCTCTTCATTCTCTCTTCCGCAGC AAGAAGCCACATTACTACAATTCCACTTCTCTCATGGCTGAATGTCTCTCCTCCTCCACCGTGGAGCCTCCTTCTCTCCT CGTCTTCTCAGGTGGTACTGGATTTAATGGTGTGGTTGAAGAGTTGAAGAAGTTAACAACACGAGTCGCTCATGTTCTTC CTGTCTCTGATGATGGTGGAAGTACTGCTGAGATTGTTCGTGTTCTCGGAGGGCCAGCGGTTGGTGATATACGTTCGAGG TGTTTAAGGCTGTCTGATGAGAGTACTTCAGAGGCACTTGCTGTTAGGCGGTTGCTTGGCCATCGCTTACCTATTGATGC ACATCAGGCCAAGAAAGATTGGTACGATATTGTAGAAGGTAATCATTCTCTGTGGGATGGTGTATCAAGACCCTACAGTG AAACAATTCGCGCTTTCTTGATATACTTTCAGAATGAGATTCATAGGCGACCTAATGAGAGATTTTGCTTCAGTAATGGA AGCATTGGGAACTTCTTCTTTGCAGGAGCAAGGATATTTTTCCAGTCTTTAGATGCTGCAATTTTTCTGTTTTCACGTGT TTCAGAGATTCCTTGTGACAGTTTGGTCCTCCCAGTGATATCAACCAATGATAGGCTAACACTAGGATGTGAATTACAGG ATGGGACTATAATACGCGGACAGAACGAAATTTCTCACCCGACCACTGGGACTACGCAGACAGTTGACAAGAGACATTGT TCTACTTCCGCTCTCCCATCGAAGATAAAGAGAGTGTTTTACATGTCAAGTGAAGGCAATAATCTGCTTCATGAGGTCTT CCCACCAGTTAACCCAACTGTCCTAGAGCAGTTGAGAAGCGTGGACTGTATCGTTTACGCAATGGGATCCCTGTTCACTT CCATTTGCCCATCACTGGTTTTGCTTGGTGTTGGGGAGATTATCTCTTCCAGGTCATGTCGTAAGGTACTTCTTTTGAAC GGTAGCCAAGACCGAGAAACAAGCAGGTTCACCGCTTCCTGCTTTGTGACTGCTATCGCTGACGCTCTCAACAGAACTCA TGGAGATACTAATATTCGGCTCAAGAATCCCCCTGGATATTACATCAACACTCTCTTGGTCCCGAAAGATGGAGAAATAG CAGTAGATCTCAAACAATTGTCTGAACAAGGGATATATGATGTTAGAGTCGTGGAGAGTGTTGGTGACCCGAAGCACGGT ATCTTGTTCAACCCGAGCTCACTGATAAACACACTGGCCGGTTTGGTGGAGTAAATAGTGATTCTTTAGGCCAGAGCTAG TTTCTTCTTCTACGCAATTGGGACCATGATTTTTTTGTTACTGTTTAGTTTGATCCTTTAGTGAATTGATATG ................................................................................ ........................................N..........N............................ .............................................N..............................i... ................................................................................ .................................i.......N...................................... ..........................N............................................N........ .........N..N................................................................... ................N...........................................................N... ........................................................N....................... .................................N....................N....................N.... ....................................................N........................... ...............................................N..................i............. N............................................................................... ..........................................i...........................N......... .............................................................N.................. .......................................................i........................ ...............................................................................N ....................................................................N........... ....................................N..N........................................ ................................................................................ .........................N............................................N.. Pos Score Pred ------------------------ 121 0.393 - 132 0.262 - 206 0.196 - 237 0.559 Yes 354 0.710 Yes 362 0.414 - 427 0.290 - 472 0.128 - 490 0.215 - 493 0.211 - 577 0.180 - 637 0.278 - 697 0.182 - 754 0.282 - 775 0.204 - 796 0.293 - 853 0.156 - 928 0.429 - 947 0.530 Yes 961 0.347 - 1083 0.571 Yes 1111 0.276 - 1182 0.424 - 1256 0.622 Yes 1360 0.115 - 1429 0.451 - 1477 0.268 - 1480 0.369 - 1626 0.044 - 1671 0.052 -

Explain the output -- Processing locus 362: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACGACTATTGTAGTTAATGAGATTTTATTTTACAATTTCTCAATTCTCCTCTCGTCCTCTATATATAGACTCGATCTTTC TCGATAAAATTTGGATATTAAACAATCATGGAAAATCATGTTATAAATTGCATTTCTAAAAAACGTCGGATGGATTATGC GGTAACCAAAGATATCCCAAATCGCAAAGGTGAGGGAAGTTCTAAGGTTGGCCAATCCACCTTTGAGACACAAAGTTGTG TGGATAATGTAGTCTACAAATTGTACTTCAAGGGTTTGGTGAGCGGTGAAACGGCGACAGACAAGAAAGCGATTGTGAAA GTTGGGTTTGGAGTCGCGATATGCGACGAGGCGGATAACTTATTCTATGGAATGAATAAATCACTTTATGAAGCTGTGAT TAACCGCGAAGAAGCAGATATTCTAGCACTAATTACTGGGTTATATGAATCAATTCACCGTGGGGTTAAAAATGTCGTGA TTTGTTGCGATGACCGTCAAATTTACCAAACCATAATTGGTAGAGAGAAGCCTCAGCAGAAGAATGTCCATCTTTTGGAA GAAGTCCAACGCCTTAGAGGGAGACTGGCTTCTACTGGAACTGTTTTGGTTGCGACGCGAGATGATAACTTTGCTCTTAG ACTTGCTATAGATGCACTTGTGAAGGCAACACAAGAAAAACCATTGACTTGTTCCATTTGTAGTGACAAAACCGACGCTG AGCACATGTTATTGAACGACAAATGCCTTCACCGTCATTGCTTTTCTTGCGTGAAACAGCAAGTGAAAGTGAAGCTTCGT AGCGGAATAGTGCCCCCGTGCCTTGAAGATGGATGCAAGTCTGAGCTCACTCTTGAAAGTTGTAGCATGGTTTTGACACC AAAGCTGATTGAGATGTGGAAACGTAAAATGGAGGAGGACTTAATTCCCGATGCTGAGAAAATCTACTGCCCATATCGAA GCTGCTCAATGTTGATGTCCAAAACCGAGCTTTCAAGAGAAGCCGAACAATCCAATGTCCGAGCATGTATCAAATGCTCT GAACTCTTCTGCATTGACTGCAAAGTACCATGGCATTCTGATTTGTCCTGTGCTGATTACAAGAGAATTCACTCTGAACG TCTGGTTAATGACATGATGCTTAAGGTTCTGGCAAACGACCAAATGTGGCGTCAATGTTCCGAGTGTAAGCACATGATTG AACTGACTGAGGGTTGCAATCACATAACTTGCAGATGTGGATATGAGTTTTGCTACAGATGTGGGCACAAATGGACTAAG TATCATCATTACAGTTGCCAACTTATGGATAATCGTGAAGATGAAGAAGATTATAATCTTCATGTTGATGCTGAGGTCAA CAATGATGATGATGACGATGAAGATTATGTATTTGATGAAGATTATGAGGAGGATGACGATGATGACGACGATGATGATG GCACAACATACAGCTTAGAAGACTTTCGAAGAGAGATTTCGTGAATCATCGAGACCAATATATGTTAATCCAAAGAGATT AAATTATGTCATATGACCAGAGAGATAAGAGCAGATAAACTCCCAGATAATTTTAAGCTTTAA ................N............................................................... ...........................i.........N...............................i......N... ................................................................................ ......N......................................................................... ....................N.........................N....N...............N............ ............................................N..........................N........ .........N.....................................................N................ .............................................................N.................. ...........N.................................................................... .....i................N......................................................... ............................N...N.................................i............. .............N..............i.....................N............................. ........N.....N.......................................i.........N........N...... .............................i.................................................. ........N....N..N..........................i..........N..................N...... ..................................N.......i...............N...........N......... ........................i...............N....................N.....N............ ..N..N..N..N.....N........N........N........N........N.....N..N........N..N..N.. .............................................................N.................. .....N......N.................................................. Pos Score Pred ------------------------ 17 0.293 - 108 0.713 Yes 118 0.339 - 150 0.584 Yes 157 0.289 - 247 0.327 - 341 0.254 - 367 0.228 - 372 0.434 - 388 0.159 - 445 0.175 - 472 0.449 - 490 0.234 - 544 0.364 - 622 0.175 - 652 0.199 - 726 0.636 Yes 743 0.430 - 829 0.441 - 833 0.445 - 867 0.694 Yes 894 0.270 - 909 0.823 Yes 931 0.278 - 969 0.491 - 975 0.187 - 1015 0.629 Yes 1025 0.213 - 1034 0.109 - 1070 0.682 Yes 1129 0.215 - 1134 0.355 - 1137 0.353 - 1164 0.625 Yes 1175 0.459 - 1194 0.183 - 1235 0.145 - 1243 0.523 Yes 1259 0.104 - 1271 0.081 - 1305 0.791 Yes 1321 0.310 - 1342 0.112 - 1348 0.105 - 1363 0.209 - 1366 0.343 - 1369 0.169 - 1372 0.208 - 1378 0.225 - 1387 0.221 - 1396 0.107 - 1405 0.243 - 1414 0.142 - 1420 0.173 - 1423 0.082 - 1432 0.120 - 1435 0.130 - 1438 0.203 - 1502 0.098 - 1526 0.101 - 1533 0.124 -

Explain the output -- Processing locus 363: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCCGTCGAGTAATTGAAATCTCTCTCAGCCGTTGGATTCGAATTCCGCCGCATCTTCATATACCTCGTTTCAATCCTGCG GAACCCTAGTTGCTCTGGTGAGTCCCTGGTGAACCCCCGGATTTTAGAATCATCTATTGTATTTACTTTAATTCCGTTCC ATTGATGATTTCTGCGTTAGATTTTGAGGTCTCTGCCTTCACCTCTATGTATTATAATGGCTCTGATTCGAAGTTCACAT TCTGTTTGTTTATATGCAGAATTGTGGTTTGCTACTGTGATAAGTTCTTCAGTGGTAGATTAGGAAGCACCACCAATGGA GATTGTGTAAGACATATTCATTACAGCTCTCTTTTAAGGATTATGGGGTCTGTTTGCTGTGTGGCTGCAAAGGACAGAAA TGTTCCCAGTGGAGCTATTGATAATTCAGTGTGTTCACCATCGTGGAGTTTTCGACGGGACAACAGGAGGCGTGTAGCTG ATGAGATTAAAGATTCATCTAATCATAATGTTGGGAGTAGAGGTATTGACATCGATAAGTTGTCGTTAGGCTTAGAGAGA GGACCTCCGTCATCTGAAACTGGGGGTTTAGCTACTCTTGGCTCTCAGAAATCCGCAGACTCAGAGATGGGCACAGCTTC GATGGTTACAGCTCCTTTAGCGGGTACAAGTTTGGCGATTCGATCTCCTTCAGATGTATCCTTGGCAAGTCCTGTTCGTG TTGAGGTGAAGAATATAGTTGACTCATCAGATATTGTTTCATCAGTTTTACCAAATCCGTCAAGTTCCACCTCGGTCTCT GATCTTCCTTCAGCTCACACACATTCACTTCCTCCCAGATCAACTCCGTCTAGACGTGCTCGAGGTTCACCTGGGCAGCA GTTGTTTAGGCAGGTTTCTGATAGTCAAACTTTGGGATTGAAATCTCCCAATAATTACTCAACATCTGAAGGAAGGTCGT CCTTTGTGCTTTCGACCTGTAGTAATGACACTGCCACTGGGTCCCATTTTGCTTCTTCTGAAGGTGGCTGGTCCATAAAC GCCTTTTGTGAGCTCGTGGCTCAGTCTCAAAGGGAGAGATGGTCTTTTGATAATGAGCACTTAGGTTCCGGACGACGAAG ACTAAGTGGGTGTAGTAGTAGCAGATTCTCATGCTCCCCTTCTGTTGATCAGCAGACTTGTGGGCGTTGCTCAAAGCTTT TAACTGAAAGGTCTCCAGTTGCCAGGTTTGACCTTCCAATTCCTGCTGTTCTAGCGTGCGGCCATGTTTACCATGCGGCA TGCTTAGAGACAATGACAAATGAAACAGAGAAGTATGATCCTACGTGTCCCATATGCACAGAGACACAGGTTACTAAATT ATCGAGGAAAGCTCTAAAAGCCGAGGCAGAATTGAAGGCAACGAGTTACAAAAGGTGTAAGAACCGTGTGGTAGATAGTT ATGTCCGCAGTGAATGTGAGGATCTTATGTTCCAGAATCTAGGGAAACGAGAAGGAAAAGGTCTGAAGATGGATCCTAGT TCGAATACCAAAGGCTCCACGTCTAAGTCTTTTATGAAATGGCACTTCGGTTCCGTCAGCACCAAGTGGAGTAAAGCCAG GGATTCTACCTCGAAGAAGAGCTTTTGGTCCAGACATAGTAATAAAAGATTAAGTCATTCTTTTTCAAGCGTTGAGGGCC TTAATCAGGCAACATGACATAAAATAGGACTGGCTTTGGATTAGAGAAAAGCTCCCTTAAACATTTTGTATCCCTTTTTA CTTTTATTCCATGTTTAAATGTCACATGGGGAAAAT ................................................................................ ................................................................................ ....N.........................................N.........i....................... .............N.............................................................i.... ..........................................i....................................N ................................................................................ N..........................N.................................................... ..................................................................i............. ................................................................................ ................................................................................ ................................................................................ ........................i....................................................... ......................................i.............i........................... ..............................N................................................. ...............................................................N........N......N ............i......N..............N..................N.......................... ................................................................................ N............N............N.........................................i........... .................................N....N......................................... ................................................................................ .............i.................................................................. ..........N.......N......N.......... Pos Score Pred ------------------------ 165 0.356 - 207 0.308 - 217 0.674 Yes 254 0.162 - 316 0.717 Yes 363 0.772 Yes 400 0.394 - 481 0.145 - 508 0.411 - 627 0.651 Yes 642 0.772 Yes 694 0.118 - 985 0.662 Yes 1079 0.534 Yes 1093 0.626 Yes 1151 0.102 - 1264 0.184 - 1273 0.323 - 1280 0.329 - 1293 0.686 Yes 1300 0.257 - 1315 0.231 - 1334 0.432 - 1441 0.481 - 1454 0.248 - 1467 0.156 - 1509 0.538 Yes 1554 0.369 - 1559 0.313 - 1694 0.731 Yes 1771 0.030 - 1779 0.071 - 1786 0.053 -

Explain the output -- Processing locus 364: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGAAGATACATAAAAACCCTAAATGAAGCTGGGCCAGTAGTGGGCTTTTGAATCTAGTTGGGCTTTTACAGATGTTATA TACCGACAAGATCGTATATGACATGTCTTCCAGTTTTATCGAATTGAAGAAATGGCAAGATCTTCGTCATGGCACCGAAT GTCAAATTTCATGAGAAAGTATCGAAAAATTCCTCATTCGTCTTTCAAAACCAAATGGAACGAGAATCTCAAGCAGAAGT ATGCCATGGAAGAGCTAAGAAGCAACCTTCTCACAGATTCAGAGAATGCTTCTGTTATGCGAACCCTCTTAAGCTCATTC CAACTCCATAACTGTGAGCCCACTCCTCAAGCTTACAGATTCGTTATCAAAACCCTAGCTAAATCCTCTCAGCTTGAGAA TATTTCATCTGTTCTTTACCACCTTGAAGTCTCTGAGAAGTTCGATACACCTGAATCCATTTTCAGAGATGTCATCGCTG CTTATGGCTTCTCCGGTAGAATCGAAGAGGCGATTGAGGTTTTCTTCAAAATCCCTAATTTCAGGTGCGTGCCTTCTGCT TACACGCTCAATGCTTTGCTCTTAGTTCTTGTGAGAAAAAGACAGAGTCTTGAATTGGTTCCTGAGATTTTGGTGAAAGC TTGTAGAATGGGTGTTAGGTTAGAGGAATCTACTTTCGGGATATTGATCGATGCGCTTTGTAGAATAGGTGAGGTAGATT GTGCTACTGAGTTAGTGAGATACATGAGTCAGGATAGTGTTATTGTTGATCCAAGATTATACTCTCGGTTACTGAGTTCT GTATGTAAACACAAGGATTCGTCATGTTTCGATGTAATTGGATATTTGGAAGATCTGCGAAAGACTCGGTTTTCGCCAGG TTTGCGGGATTATACAGTTGTGATGAGGTTCTTGGTGGAAGGAGGAAGGGGGAAAGAAGTTGTAAGTGTACTGAATCAGA TGAAATGTGACAGAGTAGAGCCTGATCTTGTTTGTTATACAATCGTGTTGCAAGGCGTTATTGCGGATGAGGATTACCCG AAAGCAGACAAGTTGTTTGATGAGTTGCTCTTGTTAGGTTTGGCTCCTGATGTTTATACCTATAATGTGTATATCAATGG GTTATGCAAGCAGAACGATATTGAAGGCGCCTTAAAGATGATGTCTTCAATGAACAAGCTAGGTTCTGAGCCTAATGTAG TTACATACAATATATTGATCAAGGCATTGGTAAAAGCTGGAGATCTGAGTCGAGCTAAGACTCTATGGAAAGAGATGGAG ACGAATGGAGTTAACCGGAACAGCCACACATTTGATATTATGATTAGTGCATATATTGAGGTAGACGAGGTTGTTTGTGC TCATGGTTTATTGGAAGAAGCATTTAACATGAACGTGTTCGTTAAGAGTTCGAGAATTGAGGAAGTCATTAGCAGATTAT GTGAGAAAGGTTTGATGGATCAAGCAGTCGAACTTTTAGCACACCTGGTTTAAGCAATGTGGAGCATCTCTAAATAGTTG TAAAATTGATGTATTTGGTCTTATTGATGATTGTTAGAACTTAATAGAAGAGTGATTAGTTTGCTTGGCAAG .......................N................................................N....... .................N....N............................i................N.........N. ..........N...........................................N......................... i....i.......................................N..........N....................... ................................................................................ ....................................................................N........... ...N............................................................................ ..........N..................................................................... .......i..........................................N............................. .......................N........................................................ ..N....................i.......N................................................ ......................N........................................................N ....N.............................................................N............. ...................N.............................N..............N...........N... ...N.................................i..i........i........................N..... ................................................................N.........i..... ....N..................................N........................................ ..N.........................i.................................................N. ..............N.........................................N....................... ........N.................N............................................. Pos Score Pred ------------------------ 24 0.329 - 73 0.252 - 98 0.230 - 103 0.309 - 132 0.637 Yes 149 0.444 - 159 0.350 - 171 0.388 - 215 0.319 - 241 0.644 Yes 246 0.633 Yes 286 0.384 - 297 0.380 - 469 0.263 - 484 0.223 - 571 0.121 - 648 0.776 Yes 691 0.240 - 744 0.273 - 803 0.252 - 824 0.701 Yes 832 0.377 - 903 0.355 - 960 0.500 - 965 0.194 - 1027 0.266 - 1060 0.120 - 1090 0.112 - 1105 0.254 - 1117 0.101 - 1124 0.462 - 1158 0.805 Yes 1161 0.621 Yes 1170 0.577 Yes 1195 0.092 - 1265 0.268 - 1275 0.676 Yes 1285 0.177 - 1320 0.472 - 1363 0.117 - 1389 0.695 Yes 1439 0.386 - 1455 0.105 - 1497 0.112 - 1529 0.062 - 1547 0.031 -

Explain the output -- Processing locus 365: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATACAAATGAACTAAATCACCTATTGGAAAAACAAAACGTGTTTTTTTTTTAATCACTGTGGCAATTTCGTAGTTTG ATAAAGAAAGGTCTTCTTTAAAAGCAGGGCGAGAGAATGAGTGTCGAAAAATCAAGGAAAAAGGGACAAACTTCAGAAAA GCTTATGGATCCGATGGAATCTTTGCCCGAAGCTGCCGCACAGATCGATCTTTGGGATGATCTTGCGGCTTCCCTTGTCA AAATCTCGAAATCACATGCCTTTGTGCCGGAGGAGCAGCTATTGAAATTTGTCGATTACCTTGAGATAAAACTTTCCTCT GTGCAATATCTATGGCTGGACATGTTCAAAGGGTCTCCTTGCCCCAAGCTGATAGATATGATTGATGTTCCATTATCTCA TATTCCTGTATCTGTCTACGACACATCAGTCGAGTGGCTCGACAAATTTTCTATTGGGTTACTCTGTGCCTTTGTAGTGT GGTCGTTGAATCGTCTTCTCACGATTTTGGAACCACCACAACAGGGAGGTCACCAGCGACGTACTACTTCCAAATTTCAC GTAGCAGTATTTGTTGCACTAGCAATGGTATTGCGTAATGAACCTAATACTTTGGTTATTGTATTGCCGACTCTCAAGGA AGACGAGTATCAAGGACATGACAAGCTTCCGATTTTAGTTTGGATGATGGCTCAGGCCTCCCAAGGTGATTTATCTGTAG GCTTGTATTCATGGTCGTGTAACTTGTTACCAGTATTTTACCAAGAAAACTTGTTACCAGTATCCCGTAGCAACTCTCAG TCAATGGATCTCATCCTGCAGTTGGCTGAGATGATTTTGTCTAATCTTGATGCTCGGACTATACTTGTAAACGGAACCGT TATAGATAAACAGCGACTGATTTCACCTTATGCATTTGAGCTCTTGATGCGGCTTACATTCCCTGCTTCATCTGAAAGAG TAAAGGCTACAGAGAGGTTTGAGGCAATTTATCCCTTGTTGAAGGAAGTGGCTCTTGCCTGTGAACCAGGAAGCGAGTTA ATGAAACAAGTCACTCAACAAATATTCCATTATTCTTTGATAATAGCTGGAAGAAGAAATCTTGTTTTAGCCAAGGAAGC TACAGCAATCGCTGTCTGGTCTCTGACCGAAAACGTAGATTGCTGCAAGCAGTGGGAAAAACTCTATTGGGAGAATAAAG AAGCTAGTGTTGCTGTTCTTAAAAAGCTTGTAGATGAAAGGGGTGATATTTCCATCAAACTAGCATCATCACCGAGTCAT AAATTCACACTCAATCAAACTATGGAGAGCATCAGGCTAAAAAACGAAAAAGCCATCACTGAAGGAGGAACCATTGGTTC TCGTTACAAAGAAGCTGACAAGTCCTGCAAAGTGATGTCGGGTAGATTCTTCCCTGAAAATGCCTGCCTCAAAGGTACAG CCATTATTACTGCTGTGGTTCTAGCTGCTGTAGCTGTACTAGCTTCCAACCTGTAGGCTGAAGAAGATGGTGGACTCATT GGTTCTGCACCTATACTTCAATGCAAACACCTCAGCTTTGAAGAACTAAGAGAGATATACCATTGTCCTTGCTTT .........N...................................................................... ....................................i........................................... ....i........i..........................................N....................... ...............N................................................................ ...........i.........i...................................N......N............... ................................................................................ ................................................................................ ........................i............N.......................................... .................N.........................N..N................................. ..........N..................................................................... ...N..........................N..................N.............................. .............................N................N................................. ................................................................................ N............................................................................... ................................................................................ .................................N.............................................. .....................i.......................................................... ..................................i........................i.................... ..................................................................N............. ....................N...................................................... Pos Score Pred ------------------------ 10 0.488 - 117 0.528 Yes 165 0.823 Yes 174 0.633 Yes 217 0.095 - 256 0.302 - 332 0.644 Yes 342 0.623 Yes 378 0.423 - 385 0.200 - 585 0.570 Yes 598 0.242 - 658 0.292 - 684 0.281 - 687 0.295 - 731 0.248 - 804 0.375 - 831 0.437 - 850 0.110 - 910 0.110 - 927 0.427 - 1041 0.267 - 1234 0.215 - 1302 0.712 Yes 1395 0.763 Yes 1420 0.516 Yes 1507 0.152 - 1541 0.033 -

Explain the output -- Processing locus 366: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTATTAGGGTTATGTATTCTCATCGTGATCCTAGTGTTCGCCGAAGTGGCGCTGGCAACATTTTTATTAAGTACGCTTGT CTCTATCCTTCTGACATTTGAGTTGCTGTAAGACGAGATGATTGTTGCGAAAAAATTTGTATCTTGTATTATTCATTGAA AAGTCTGGCAAAAGTAGTTGATTTCTTTAGTTATGTAAGTTTTTTTATTGGGTGTTCTTTCTCTCTTTCTGATGCTAAAC CTGGCTATAATATGCAGAATTTGGACGAGTCCATCGACCACAAAGCACTGCATGATACTTTTTCATCGTTTGGAAACATT GTGTCGTGCAAGGTAGCTGTCGATTCTTCAGGCCAGTCAAAAGGCTATGGGTTTGTGCAATACGCCAATGAAGAATCTGC CCAGAAAGCTATAGAGAAACTGAACGGCATGTTGCTCAATGACAAGCAAGTGTATGTGGGTCCTTTCCTGAGGAGACAAG AAAGAGACTCCACTGCTAACAAAACGAAATTCACCAATGTGTATGTGAAGAATCTCGCGGAAAGTACTACCGATGATGAC TTGAAGAATGCTTTTGGCGAGTATGGAAAGATAACAAGTGCTGTCGTGATGAAAGATGGAGAAGGGAAGTCCAAGGGCTT TGGGTTTGTCAACTTTGAAAATGCTGATGATGCTGCTAGGGCTGTGGAGTCTCTCAATGGGCACAAATTTGATGATAAGG AGTGGTATGTTGGTAGAGCCCAGAAGAAGTCAGAGAGGGAAACAGAATTAAGGGTCCGTTATGAACAGAATTTGAAGGAA GCTGCAGACAAGTTTCAAAGTTCAAACTTGTATGTTAAGAATTTGGATCCTAGCATTTCAGATGAGAAACTTAAAGAGAT CTTTTCTCCTTTTGGTACCGTTACATCTAGCAAGGTGATGCGGGATCCTAATGGAACAAGCAAAGGCTCAGGTTTTGTTG CTTTCGCAACTCCCGAAGAAGCAACTGAAGCTATGTCACAGTTGAGCGGTAAAATGATCGAAAGCAAGCCACTCTATGTG GCTATTGCACAGCGGAAGGAAGACAGAAGGGTCAGACTACAGGCTCAGTTTTCCCAAGTGAGGCCAGTTGCAATGCAGCC GTCTGTTGGTCCCCGCATGCCAGTGTATCCCCCGGGTGGTCCTGGTATTGGACAACAAATGTTCTATGGTCAGGCCCCTC CTGCCATGATTCCTCCCCAGCCTGGGTATGGATACCAACAGCAGCTTGTTCCTGGAATGAGACCTGGTGGGGGTCCTGTA CCCAGTTTCTTCATGCCTATGGTTCAGCCACAGCAGCAGCGTCCTGGAGGAGGAAGACGTCCTGGGGGAATCCAACACTC CCAGCAGCAAAATCCCATGATGCAGCAACAGATGCATCCAAGGGGTCGGATGTTCCGGTATCCCCAAGGGCGTGGTGGTA GTGGTGATGTGCCTCCATATGATATGGGCAACAACATGCCATTGACTATTGGAGCTTTGGCTTCAAATCTGTCTAATGCT ACTCCAGAGCAACAGAGGACGATGCTGGGTGAGGTGCTGTACCCGTTGGTGGAGCAGGTTGAGGCAGAGTCTGCAGCCAA AGTGACTGGGATGCTTTTGGAGATGGACCAGACTGAAGTGCTCCATCTGTTGGAGTCACCAGAAGCTCTCAAGGCCAAAG TTGCAGAGGCTATGGATGTTCTCAGGAGTGTCGCTGCTGGTGGTGCAACCGAGCAGCTCGCTTCCTTGAACCTCTCTTAA ATTGCTTTTTATCATTTGATCTTTGGCTTTTGTCTCTCCCATACAATTGGAGTTGTTTCTTTGCTATTCGTTTTCTCCCT GGGAATTTGGATTCCTCCA ...........i.................................................................... .....................................N.......................................... ................................N......................................N........ ...........i.......................................N............................ ..............................................N....................N............ ............................N.........N..............N.......................... ....................................N.....N.............................N..N.... .......N..............N.........................i......N........................ ....................N.....N..N..........................N..............N........ ......N.....................................................N................... ...............................N.............................N.................. .....................................i............N............................. ................................i....................i.....................N.... ........................................................................i....... ................N.........................................i......N.............. .....i.....................N............................i....................... ............N.....N............................................................. ................N..i...........i.................N.............................. ......N...........N....i...........i.......................................N.... .....................i.......................................................... ..........N...........i......................................................... ...........i...N................................................................ ................................................................................ ................... Pos Score Pred ------------------------ 12 0.615 Yes 118 0.206 - 193 0.318 - 232 0.129 - 252 0.599 Yes 292 0.249 - 367 0.238 - 388 0.323 - 429 0.421 - 439 0.407 - 454 0.182 - 517 0.280 - 523 0.144 - 553 0.322 - 556 0.247 - 568 0.134 - 583 0.117 - 609 0.720 Yes 616 0.357 - 661 0.467 - 667 0.077 - 670 0.294 - 697 0.189 - 712 0.161 - 727 0.127 - 781 0.164 - 832 0.082 - 862 0.306 - 918 0.731 Yes 931 0.329 - 993 0.746 Yes 1014 0.594 Yes 1036 0.424 - 1113 0.793 Yes 1137 0.473 - 1179 0.689 Yes 1186 0.194 - 1206 0.593 Yes 1228 0.443 - 1257 0.636 Yes 1293 0.324 - 1299 0.266 - 1377 0.292 - 1380 0.710 Yes 1392 0.630 Yes 1410 0.363 - 1447 0.157 - 1459 0.062 - 1464 0.684 Yes 1476 0.729 Yes 1516 0.156 - 1542 0.700 Yes 1611 0.342 - 1623 0.543 Yes 1692 0.556 Yes 1696 0.176 -

Explain the output -- Processing locus 367: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCCACATAGATCTAAAAAAAATTATATCATGATGTATAATCTTTGGTATCAATAATTGTATGTTGGTTTCAATTACAAGC CCAACTATTGTTTGGGCCTAATATTTATATATGGGCCTTAGTCAAAGATTAAAAAAAAAACATTTCTTCTTCCCAAAGCC AGAAAATATCAGTACAAGAGTACAAGAGAGAAAGAAAGGAACCTCGAACTCAGAGAAATTGAGAGAAGAGAGAAGAATCA TGGGACAAGGACCATCGGGAGGATTGAATCGGCAAGGAGATCGGAAACCTGATGGTGGAGATAAGAAAGAGAAGAAGTTT GAGCCGGCGGCACCACCGGCTCGTGTAGGGAGGAAGCAAAGGAAGCAGAAAGGGCCAGAGGCGGCGGCGAGACTTCCGAC GGTGACTCCGTCTACTAAGTGTAAGCTCCGGTTACTGAAATTGGAACGGATCAAGGATTATTTGTTGATGGAGGAAGAGT TTGTGGCGAATCAAGAGAGGTTGAAACCTCAGGAAGAGAAAGCTGAGGAAGATAGATCTAAGGTTGATGATCTTCGTGGT ACACCTATGAGTGTTGGGAATTTGGAAGAGTTGATTGATGAGAATCACGCTATTGTTTCTTCTTCTGTTGGACCTGAGTA CTACGTTGGGATTTTGTCCTTCGTTGATAAAGATCAGCTTGAACCTGGATGTTCGATTTTGATGCATAATAAGGTTCTCT CTGTTGTTGGGATTCTACAAGATGAAGTGGATCCAATGGTGTCTGTGATGAAAGTTGAGAAAGCTCCTTTGGAGTCATAT GCTGATATTGGAGGTTTAGAAGCTCAGATTCAGGAGATTAAGGAAGCTGTTGAGCTTCCTTTGACTCATCCTGAGTTGTA TGAAGATATTGGTATTAAACCACCAAAGGGTGTAATTTTGTATGGTGAGCCTGGAACTGGGAAGACATTGCTTGCTAAGG CGGTTGCGAATTCAACATCAGCTACTTTCTTGAGAGTTGTTGGTAGTGAGCTGATTCAGAAGTATTTGGGAGATGGTCCT AAGCTTGTGAGAGAACTTTTCAGAGTTGCTGATGACCTTTCACCATCAATCGTCTTCATAGATGAGATTGATGCTGTTGG TACCAAGCGGTATGACGCTCACTCAGGAGGTGAACGTGAGATCCAAAGAACTATGTTGGAACTTCTGAACCAGCTTGATG GATTTGATTCAAGAGGAGATGTTAAGGTCATCCTTGCAACAAACAGAATCGAGAGTCTTGACCCAGCACTGCTTCGACCT GGACGGATTGATAGGAAAATCGAGTTCCCTCTCCCAGATATCAAAACAAGAAGACGTATCTTCCAGATACACACATCGAA GATGACTCTCTCGGAAGATGTAAACCTCGAAGAGTTTGTGATGACGAAAGACGAGTTCTCAGGAGCTGATATAAAGGCTA TATGCACTGAGGCTGGTCTACTAGCTCTCAGGGAGCGTCGTATGAAGGTGACACATCCTGATTTCAAGAAAGCAAAGGAG AAGGTTATGTTCAAGAAGAAAGAAGGCGTCCCTGAAGGCCTCTACATGTAAAATTATAATCATATCGTCATCATCGGCTT TCCTCCTCCACACTTTGTTATAATACTTTTGCTTCTGCTTAGGATCTTTCTGGTCATATAAATTGATCAT ............................N..N...........................N.................... ..............................N................................................. ...............................................................................i ...................................................N............................ ................................................................................ ...................................................................i............ ..................................................................N............. ......i..............................N.......................................... ................................................N............N.................. .....................N.............i...........i..............................N. ...............................................................................N .........................................N...................................... ........................................................................N....... ...............................N.............................N........N......... ...........N........................................i........................N.. ..................N............................................................. ................................................................................ ......N......................................N.................................. ...................................................................... Pos Score Pred ------------------------ 29 0.428 - 32 0.364 - 60 0.462 - 111 0.408 - 240 0.618 Yes 292 0.336 - 468 0.611 Yes 547 0.056 - 567 0.602 Yes 598 0.085 - 689 0.278 - 702 0.450 - 742 0.194 - 756 0.818 Yes 768 0.685 Yes 799 0.060 - 880 0.050 - 922 0.192 - 1033 0.150 - 1072 0.415 - 1102 0.202 - 1111 0.274 - 1132 0.144 - 1173 0.760 Yes 1198 0.461 - 1219 0.188 - 1362 0.718 Yes 1378 0.318 - 1401 0.781 Yes 1442 0.376 - 1482 0.292 - 1527 0.424 - 1566 0.219 -

Explain the output -- Processing locus 368: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAGTTAAATTTCTTTTGCGAAGAATAGTTCAGGATGCAGCTGCGACTTTCGAAAAGCTTGAAGCATATATAATGGTGTA AAGACTAAAGAGTAAAGATAATTAAGCATGAAGACGATGTGGTGGAGAGATATGAGAAGTCTACTTATAGTTCCAATGTT CAAGTGTTTGGTGGCTATATGTTTGGTCATCTCACTATTAGTTTTCGTAGAAAGTTTATATATGAACATTGTAGTAATCT ACGTCAAGTTGTGTAAACGCAAACCCGAGAAGATTTACAAATGGGAGGCAATGCAAGAGGACTTGGAGCTCGGAAATCAA AACTTTCCCATGGTTCTTGTCCAAATCCCAATGTACAACGAACGAGAGGTGTTTAAGTTATCCATTGGTGCTGCTTGCAG ACTCATATGGCCATTGGATCGACTAATCGTTCAAGTTTTAGATGATTCCACTGATCCCACCATCATGGAAATGGTAAGCA CAGAGTGTGGAAAATGGGCAACCAAAGGCATAAACATAAAATGTGAGAGGAGAGACAATAGAAATGGCTATAAGGCTGGA GCTCTTAAACAAGGTATGAGGCATAGTTACGTCAAAACATGCACCTACATTGCAATCTTCGATGCTGATTTTCAGCCAGA GCCCGACTACCTCGAACGCACTGTCCCATTTCTTATCCACAATCCCGAACTCGCTCTTGTTCAAGCTCGATGGAAATTTG TGAACGCAAAGAAGTGCTTGATGACTAGGATGCAGGAGATGTCCCTCAATTACCATTTCACGGCAGAGCAAGAATCCGGA TCCACTAGACATGCCTTCTTTGGCTTTAATGGGACTGCGGGTGTATGGAGACTGGCGGCGATGGAAGAAGCTGGTGGTTG GAAAGACCGGACCACCGTAGAGGACATGGACTTGGCCGTTCGTGTAGGTCTACACGGCTGGAAATTTGTCTTTGTCAACG ATGTTTCGGTGAAAAGTGAGCTACCAAGCCAATTTAAGGCATTCAGATTCCAGCAACATCGATGGTCATGCGGTCCAGCT AATCTCTTCAGAAAAATGACTATGGAAATTATTCGCAATAAGAGAGTGACGATTTGGAAGAAATTGTATGTGATCTATAG TTTCTTTTTCGTAAGGAAGATCATAGTCCATTTCTTCACTTTCTTCTTCTATTGTTTCATTCTTCCTACGAGCGTATTCT TTCCCGAAGTCAACATTCCGACTTGGTCAACCGTTTATTTTCCTTTTATGATCACTCTATTCAATGCCATTGCCACACCA AGATCTTTCTACCTCGTGATTTTTTGGGTCTTATTCGAGAATGTAATGGCTATGCATCGGACCAAAGGCACTTTCATCGG TTTACTTGAAGGAGGGAGAGTTAACGAATGGGTCGTCACCGAGAAATTAGGTGATGCGCTTGAGACTAAGTTACTTCCCC AAGTAAGAAAACCTCGCAACGGGTTTCTCGAAAGGATAAACTCAAAAGAGATGATGGTGGGGATATACATATTGTGCTGT GCATCATATAACCTTGTCTTTGGGAAGACAGTGTTATATATCTATCTTTACATGCAGGCTCTTGCTTTTATTATTGCCGG AATTGGTTTTATAGGAACTTGATAAGTGTAATTAGTAATTTATATATCCGTTAGTATTCTCGTTCTTTTCTTTTTCATAT TTCAGAATAAGTAGGTGAATTATTTTATTTAATACAATTCT ..................................N.....................................N....... ...........................N........N..............N.......................N.... ..................N..........................................i.................. ........................................i.........N............................. .........N....................N................................................. ......i..................................N......................i.....i......... .............N..........................N......................i................ ...............i......................N......................N.................. .....................................................................i.......... ....................i........N........i......................................... ..........i.................N...............N...............i................... .........................N...................................................... N............................................................N.....N............ ...............N.....N.............................................N............ ................................................................................ ...............................................N...............N................ ........................................N....i.....i............................ ...........................i.........................N.......................... ..................................................N..N.......................... ...................................................N............................ ................................................................................ ......................................... Pos Score Pred ------------------------ 35 0.491 - 73 0.310 - 108 0.369 - 117 0.190 - 132 0.239 - 156 0.118 - 179 0.263 - 222 0.614 Yes 281 0.660 Yes 291 0.447 - 330 0.164 - 351 0.306 - 407 0.705 Yes 442 0.332 - 465 0.558 Yes 471 0.752 Yes 494 0.303 - 521 0.356 - 544 0.709 Yes 576 0.517 Yes 599 0.183 - 622 0.223 - 710 0.602 Yes 741 0.712 Yes 750 0.445 - 759 0.800 Yes 811 0.622 Yes 829 0.214 - 845 0.439 - 861 0.614 Yes 906 0.445 - 961 0.414 - 1022 0.426 - 1028 0.229 - 1056 0.252 - 1062 0.284 - 1108 0.282 - 1248 0.337 - 1264 0.260 - 1321 0.397 - 1326 0.882 Yes 1332 0.533 Yes 1388 0.772 Yes 1414 0.183 - 1491 0.182 - 1494 0.327 - 1572 0.366 -

Explain the output -- Processing locus 369: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGATTTGACTGGAGTTCCACCAGAACGCCAAAAGATCATGGTCAAAGGTGGTCTCTTAAAGGTAACGTTGGATTCATTGG TTGCTGCCCGGGTTTTACTGAATGCTATATTATGATCTGTGTGTCCTAGATTGTTTATTGATGTTGATGATAAAGTCTTA TCTGTTTCAATTGATGCAGGATGATGGAGATTGGGCTGCTATTGGTGTGAAAGATGGTCAAAAATTGATGATGATGGGAA CTGCTGATGAGATAGTGAAAGCTCCTGAGAAGGCCATTGTTTTTGCAGAGGATCTACCTGAAGAAGCGCTAGCCACTAAT CTGGGTTACAGTGCTGGCCTTGTCAATCTTGGCAACACGTGTTACATGAACTCCACGGTGCAGTGCTTAAAATCTGTCCC AGAGTTGAAATCTGCATTATCCAATTACTCACTTGCTGCCCGAAGCAATGATGTTGACCAGACTTCTCACATGCTCACAG TTGCCACACGTGAGTTATTTGGTGAGCTTGATAGAAGTGTCAATGCTGTTTCGCCTTCACAGTTCTGGATGGTATTACGA AAAAAGTATCCTCAGTTTAGTCAGTTGCAGAATGGAATGCACATGCAGCAGGATGCTGAAGAATGTTGGACACAACTGTT ATACACCCTTTCTCAGTCCCTAAAAGCACCAACTTCCAGCGAAGGTGCTGATGCTGTGAAAGCTCTATTTGGTGTCAATC TCCAGAGCAGGTTGCATTGTCAAGAAAGTGGCGAAGAAAGCTCAGAGACAGAATCTGTATATTCTCTAAAATGTCATATA TCACATGAAGTGAACCACTTGCATGAAGGATTAAAACATGGACTGAAAGGGGAACTTGAAAAAACATCTCCTGCTCTTGG CCGTACTGCACTCTACGTCAAGGAGTCACTTATAGATTCCTTGCCAAGGTACTTGACTGTTCAGTTCGTGCGGTTTTTCT GGAAAAGGGAGAGTAATCAGAAAGCAAAGATCCTCAGGAAAGTGGATTACCCGCTGGTGTTGGATATATTTGACCTTTGC TCTGAGGATCTTCGGAAGAAACTGGAAGCTCCTCGCCAGAAACTTAGAGAGGAGGAAGGTAAAAAGCTTGGTCTTCAAAC TAGTGCTAAGAGTGGCTCAAAGGACAGTGATGTGAAAATGACTGATGCAGAGGCGTCTGCAAATGGAAGTGGAGAATCAT CCACAGTAAACCCACAGGAAGGTACTTCTTCTGAGAAGGAAACTCACATGACTGGAATCTATGACTTGGTCGCTGTGTTG ACCCACAAGGGCAGGAGTGCAGACTCTGGTCATTATGTTGCATGGGTCAAGCAAGAGAGTGGCAAATGGATTCAATATGA TGATGACAACCCGAGCATGCAACGCGAAGAAGACATCACAAAGCTATCTGGAGGAGGTGATTGGCATATGGCGTACATCA CAATGTACAAAGCCCGCTTCGTCTCCATGTAATCTCTCTCTTTCTTTTGATGAATTTAGTTCTTGAAACATTCAGATCAT TAAAAAGCTTATGTAGACCACCTCAGAAATTTCAGTTTTACATTGTTACAA .....................................N.......................................... .....................N.........N............................N.....N............. .............i......N..N.............................N.............N..i..i...... ......N......................................................................... .............................................i.................................. ...............................................N..N...................i......... ..........................................N.........................N........... ...............................N....i.....i.........N.........N................. ..................................................N............................. ......................................................................N......... ....N.................N..............N.......................................... ................................................................................ ................................................................................ ................................................................................ .............................N.......i......N.................N................. ...............................................i............i................... ..................................N......N.......................i..........N..N ..N.............N..................................................N............ ..i.......................N......................N.............................. ..........N........................................ Pos Score Pred ------------------------ 38 0.176 - 102 0.144 - 112 0.226 - 141 0.459 - 147 0.267 - 174 0.618 Yes 181 0.109 - 184 0.322 - 214 0.138 - 228 0.490 - 231 0.689 Yes 234 0.775 Yes 247 0.128 - 366 0.501 Yes 448 0.311 - 451 0.343 - 471 0.586 Yes 523 0.398 - 549 0.442 - 592 0.371 - 597 0.620 Yes 603 0.618 Yes 613 0.349 - 623 0.489 - 691 0.393 - 791 0.477 - 805 0.214 - 823 0.203 - 838 0.294 - 1150 0.171 - 1158 0.837 Yes 1165 0.273 - 1183 0.233 - 1248 0.838 Yes 1261 0.600 Yes 1315 0.113 - 1322 0.181 - 1346 0.500 - 1357 0.449 - 1360 0.440 - 1363 0.388 - 1377 0.343 - 1428 0.291 - 1443 0.510 Yes 1467 0.135 - 1490 0.094 - 1531 0.044 -

Explain the output -- Processing locus 370: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCTAATATAAAAATTGTTTCTGGAATAATGATTTCGTCCTTTAGTATACAGAGGATCTGTTGGCATTTTCCTGCAAGTA TCTTTGGATTTTGTCTGTTAAAAGGTTCTGTTACAACAGGTTTATTCTTCTAACCAAGAAACCGAAGAAAAGCTCTCTGC GATGACTCTTAGTCGGTTTTTCGTCAGGAGGTTTCACCAGATGAGAGTTGATGTTGTCTCCAGAGATATCATCAAACCAT CATCTCCAACTCCAAACCACTTAAAGAAGTTTAAACTCTCTCTTTTAGAACAGCTCGGTCCAACGATCTTTGGTCCTATG GTTTTCTTCTATTCAGCTAACAACAGCATCAAACCAACTGAGCAATTGCAGATGCTGAAGAAGTCGTTATCCGAAACTTT AACTCATTTCTACCCTCTCGCTGGACGGCTCAAAGGCAACATAAGTATCGATTGTAATGACTCAGGCGCTGATTTCCTCG AAGCACGAGTCAATTCACCGCTTTCGAATCTCCTACTAGAGCCTTCTTCTGACAGCTTGCAACAACTGATTCCTACATCT GTTGATTCCATAGAGACACGAACCAGACTGCTTCTTGCTCAAGCGAGTTTCTTTGAATGTGGAAGCATGTCTATAGGAGT TTGTATCTCTCATAAACTCGCTGACGCAACTTCTATCGGTTTATTCATGAAGAGCTGGGCTGCAATCTCGTCCCGAGGGT CGATCAAAACCATTGGAGCTCCGGTTTTCGACACGGTTAAGATCTTCCCACCTGGAAACTTCTCTGAGACTTCCCCTGCA CCAGTGGTTGAGCCAGAGATCATGATGAATCAGACTCTTTCGAAGAGATTCATATTCGATTCTTCGAGTATTCAAGCTCT GCAAGCGAAAGCTTCAAGCTTTGAAGTGAATCAACCAACAAGAGTCGAAGCTGTTTCAGCTCTTATATGGAAATCTGCAA TGAAAGCTACAAGAACAGTTTCAGGAACATCGAAACCGTCAATTCTGGCAAACTCCGTGAGTCTACGCTCACGAGTCTCT CCACCATTTACAAAGAACTCAATAGGGAATCTAGTGAGCTACTTTGCAGCAAAAGCAGAGGAAGGAATAAACCAAACAAA GCTTCAAACTTTGGTTTCGAAAATACGAAAAGCGAAACAGAGGTTTCGAGATATCCATATACCAAAGCTTGTAGGAAACC CAAATGCCACAGAGATCATTTGTAGCTACCAGAAAGAAGCAGGAGATATGATTGCAAGTGGAGATTTCGATTTCTACATA TTCTCAAGTGCTTGTCGATTCGGTTTATACGAAACCGATTTCGGTTGGGGAAAACCGGTTTGGGTCGGGTTTCCTTCGGT TAGACAGAAGAACATCGTGACGCTTCTCGACACGAAAGAAGCTGGTGGAATTGAAGCTTGGGTGAATCTGAATGAACAAG AGATGAATCTTTTTGAACAAGATAGAGAATTGCTCCAATTCGCTTCTCTGAATCCTAGTGTGATCCAACCTTTTCTTCAT GTTCTCTGATTATAAAAACCGGTTTAGTGAGCTAAACCGGATATATTTGTTGAAAAACTGGTTCTATTTTGACTAGCCGT TGGGAGGCCACTAAAAAGGTTGAAATTA ............................i................................................... ................................................................................ .............................................................................N.. ...................................................i............................ ........................................................i....................... ................................................................................ ........................................................N.........i............. ..............................................i................................. ................................................................................ .....................i..i....................................................... ..................................................................N............i ................................................................................ ................................................................................ ................................................................................ ...i...........................................i................................ ................................................................................ .......................................................................N........ ..N...........................................................................N. ................................................................................ ............................ Pos Score Pred ------------------------ 29 0.553 Yes 162 0.655 Yes 201 0.458 - 211 0.236 - 318 0.234 - 372 0.509 Yes 457 0.513 Yes 617 0.254 - 627 0.784 Yes 687 0.624 Yes 822 0.664 Yes 825 0.577 Yes 947 0.406 - 960 0.581 Yes 1204 0.582 Yes 1248 0.740 Yes 1432 0.133 - 1443 0.403 - 1519 0.082 -

Explain the output -- Processing locus 371: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTTTGTTTGTTGTATGATCTGTCAGGAATAGTTGAATTAACCCTCAAACCGGCCGGTTAATTGTTCTCGGGAGCGACA ATAAACCGAATTTTAAACTGAAAAACCGAATCTTTGGCCGGTTATTTATCAAATTGTACCGGAAATAACCGAATGAAGCC CTCCCTTAAACGACGTCTTCTTCTTCTTTCTCGAAAATTCGCCAAAGCTTCGATCCGAAAACTTCTTCGCCAATCTTCCT TTTCTTCTCACGAATTTCTCCTTCAAGCTCCGTTCCCAGCAATGTCATGGTACAGAAGAACAAAACTCGTATTCGATTCC CTGCGACGCAACATAAACCCGAAGATTCTACCTCGATCTCATGTTACTTCGAGAATCAACAATCCAATTGGGTCTTCTAA TCCCTCAGCTAAGTTCTCTTCAATCTCTTCGCGAGAAGTAGGATTAAGATCATGGACATCTCTCGGCCGAAACACGAACA GAATCGCGTATAATCCGTTTCTGAGTCAACCCAAGAGATATTACTACGTTGATCGATACCAAGTTCGTCATTTCAAGCCA CGAGGACCAGGACGGTGGTTTCAAAACCCTAGAACTGTGTTTACGGTGGTGCTCGTTGGTTCCGTTGGTCTGATCACTTT GATTGTTGGGAATACAGAAACGATTCCGTACACAAAGAGAACACATTTCATTCTCTTATCGAAACCTATGGAGAAGCTTC TTGGTGAAACTCAATTTGAGCAGATTAAGAAAACTTACCAAGGGAAAATTCTTCCTGCGACTCATCCTGAGAGTATCAGG GTTAGATTGATAGCTAAAGAGGTTATTGATGCGTTACAGAGAGGTTTGAGTAATGAGCGTGTATGGAGTGATTTGGGGTA TGCTTCTACTGAGAGTAGTTTGGGAGGTGGTAGTGATAAAGGAGTTAAGGAAATGGAAATGGCTATGAGTGGTGAAGATA CTATGACTGATATGAAATGGTCTAAAGAAGATCAGGTTCTTGATGATCAATGGATTCAAAAGAGTAGGAAGAAAGATTCA AAGGCTCATGCAGCGACTAGTCATCTTGAAGGTATTAGTTGGGAGGTTCTTGTGGTTAATGAGCCTATTGTTAACGCGTT TTGTTTGCCTGCTGGGAAGATTGTGGTGTTCACTGGGCTGCTTAATCATTTCAAGTCAGATGCTGAAGTTGCCACTGTGA TTGGGCATGAGGTTGGTCATGCGGTGGCCCGACATGTAGCTGAGGGAATAACTAAGAACTTGTGGTTTGCTATCCTTCAA CTGGTCCTATATCAGTTTGTCATGCCTGATCTTGTGAACACTATGTCCGCCCTTTTCTTGAGGCTTCCTTTCTCCAGAAA GATGGAGATAGAAGCAGACTACATCGGTTTGCTGTTACTTGCATCTGCGGGCTATGACCCACGAGTAGCTCCCACAGTGT ATGAGAAGCTGGGAAAGCTCGGTGGAGACGCACTCGGAGATTATCTATCGACCCATCCGTCGGGAAAAAAGAGATCAAAA CTTTTGGCTCAAGCCAATGTGATGGAAGAAGCACTAATGATATATAGAGAAGTCCAAGCAGGTCGTACTGGCGTTGAAGG CTTTCTTTAGCTCTTCACCATTCATCTCGTCTGCTTCTGTACATTATTTCTCCTCCACCTCCATTAATCATGTGGAATGT TATGTTAGTATGTTTATGTCGTTAAATTG ...............N................................................................ ........................................................................N....... ................................................................................ .........................................i....N................................. ........................................N....................................... ...................................................N............................ ................................................................................ ................................................................................ ...................................................................i............ ................................................................................ ............................N.......................N.........N................N ....................................................i.....i.....i............... ..i........N....N.........................N......N.............................. .......N..................................................N..................... ...........................................................N.................... ......N...........N..............N.............................................. .....................i....................i..................................... N............................................................................... ................N....i..............i........................................... .....................................................................N......N... Pos Score Pred ------------------------ 16 0.306 - 153 0.445 - 282 0.608 Yes 287 0.356 - 361 0.348 - 452 0.240 - 708 0.769 Yes 829 0.313 - 853 0.489 - 863 0.170 - 880 0.113 - 933 0.758 Yes 939 0.843 Yes 945 0.856 Yes 963 0.502 Yes 972 0.298 - 977 0.399 - 1003 0.082 - 1010 0.222 - 1048 0.078 - 1099 0.057 - 1180 0.087 - 1207 0.264 - 1219 0.085 - 1234 0.122 - 1302 0.510 Yes 1323 0.555 Yes 1362 0.851 Yes 1414 0.535 Yes 1441 0.215 - 1537 0.244 - 1542 0.591 Yes 1557 0.593 Yes 1670 0.068 - 1677 0.073 - 1682 0.044 - 1690 0.053 - 1696 0.030 -

Explain the output -- Processing locus 372: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGAGTTTTATAAACTCTATACCTCACACTGAATAATTTGGATTCTGAATGTTTTTTTTGAGTCTTTGAAATTGTTCTGT TTATTGGTATAATTTCAGTGAAGTATTAAAGATTCAGTGAGATTGACGTTAACTTATAACTTGTTGTTGCTTTTGTATTT TGACATGTTTCAGAACATGGTATCGTCTCGGGCCGTTGTGATGGCCATTATGGTATTGGTCACAGTGCAGTGGTTGGTTT TTGCTGAAGGTTATCCGGAAGAAGACTTGGTGGCCAGGCTACCTGGTCAACCAAACGTCGGGTTCAGACAATTTGCTGGA TATGTCGATGTGGACTCGGAAAATGGTCGTAGTCTTTTTTACTACTACGTGGAGGCTGTAAAAGAACCTGACACTAAGCC CTTAACCCTTTGGCTCAATGGAGGTCCAGGTTGTTCTTCAGTTGGTGGAGGAGCTTTCACTGAGTTAGGTCCATTTTATC CTACGGGCGATGGCCGTGGCCTTCGCTTGAACTCCATGTCTTGGAACAAAGCTTCGAACTTGTTATTTGTGGAGTCACCA GCCGGAGTAGGATGGTCATACTCTAACAGAAGTTCGGATTACAACACTGGGGACAAGTCTACTGTCAATGATATGCTTGT GTTCTTGTTGAGATGGTTCAACAAGTTCCCAGAGTTGAAGTCTCGCGACCTCTTTCTCACTGGCGAAAGCTACGCAGGGC ATTACATACCTCAACTGGCTGATGTGATACTCAGCTACAATTCACGCTCAAGTGGGTTCAAATTTAACGTCAAAGGCATT GCAATTGGGAATCCACTTCTGAAGCTTGACAGGGACTTTGCAGCTGCATACGAGTACTTCTGGTCACATGGGATGATCTC TGATGAAGTAAGACTCACAATCATGAACCAATGTGACTTTGCCAATCCAAAGAACATGAGCAATGCTTGTATATATGCAA TCGTTGAATCCAGCGTCCTCACTGAGTATATCAACAGCTATCACATTCTCCTCGATGTATGTTACCCTTCTATTGTTCAG CAAGAGCTAAGGCTCAAGAAAATGAATGCGCTTCACGCTAACCGCACTCGCCTACCTTATGAATGGACTATGTGCAGCAA CCGGTTAAACTACAGTGGCATAGACGGGTACATTGACATGCTTCCGAGTCTTAAGAGAATTATTCAGAACCAAACTCCTG TTTGGATCTTCAGTGGAGATCAAGATTCTGTAATTCCACTTCAAAGTTCAAGAACACTCGTACGAGAACTTGCTGAGGAT CTCAACTTCAAGACCACTATTCCCTATGGAGCTTGGTTCCACAAAGAACAGGTTGGTGGCTGGGTCACAGAGTATGGGAA TCTACTGACCTTTGCAACGGTGAGAGGAGCGGCTCATATGGTGCCGTATGCGGAGCCCTCACGAGCTCTGCACATGTTCA GTAGCTTTATGAATGGCCGGAGATTGCCCAACAAACCAGATTTAAAGTCTTCTACTGATGATTGAAAGAGAAGATATCTC TTTGAGTAAGTTGAGATAGAAACATATTAGGGTTTGTGTAATAATGAACCGTGAACCCCACGGAACTATTTTTATATACT GCCA ................................................N............................... ................................................................................ ....i...........i.......................i........N.............................. ................................................................................ .................N.............................................................. .........N.........................i............................................ ...........N.......................................................N....N....... ............i................................................................... .....................N.......................................................... ...................................................................N....i....... ..N...................N.......N........................i......N...........N..... ......................................................N...i..................... .....................i...i................................N...i......N.......... .....................................i.......................................... ................................................................................ .........................N...............................................N...... .....................................N.........N.........................N...... ........N...N............................................N...................... ...........................................N.................................... .... Pos Score Pred ------------------------ 49 0.147 - 165 0.642 Yes 177 0.616 Yes 201 0.675 Yes 210 0.339 - 322 0.239 - 328 0.265 - 343 0.325 - 418 0.423 - 490 0.475 - 516 0.670 Yes 572 0.390 - 628 0.328 - 633 0.425 - 653 0.633 Yes 742 0.164 - 868 0.109 - 873 0.609 Yes 883 0.354 - 903 0.462 - 911 0.261 - 936 0.507 Yes 943 0.456 - 955 0.283 - 1015 0.390 - 1019 0.578 Yes 1062 0.587 Yes 1066 0.664 Yes 1099 0.274 - 1103 0.588 Yes 1110 0.362 - 1158 0.535 Yes 1306 0.406 - 1354 0.315 - 1398 0.309 - 1408 0.166 - 1434 0.316 - 1449 0.147 - 1453 0.365 - 1498 0.301 - 1564 0.063 -

Explain the output -- Processing locus 373: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATCAACGAAGCAAACTCTCATTTCCTTGAAAGTGTACTTTTTTATAAGTCAATATTATTACAAGACTAGCGAAGAGTGT TTCTTTTTTTGGTCACAGTGATTGATGAATTTGGAGGAACATTTATCGTTAGGTGAGTTTCATGTGTCGAATCTTATAAA GAATCATATATCTAGAGGATCACCAATACAAGCTCTGGTTTTGTACGGAGGAATCCGCCGCAGAGGAGTGTATTTTCCGG GATGGGTTCCTTTGATTTTAAGGGCATGTGCTTGCGTTGTTCCACGTGTTGTTCTTGGGAAGCTGTTGCATTCTGAATCA ATCAAGTTCGGTGTTTGTTCTGATGTTATGGTGGGAAGCTCTCTGATCAGTATGTATGGGAAATGTGGATGTGTAGTTTC TGCACGTAAGGTGTTTGACGAAATGCCTGAGAGAAACGTGGCTACTTGGAACGCTATGATTGGTGGGTATATGAGCAATG GTGATGCAGTTTTGGCTAGTGGATTGTTTGAAGAGATATCGGTTTGTAGAAATACGGTGACTTGGATCGAGATGATTAAA GGGTATGGGAAGAGAATAGAGATTGAGAAAGCTAGAGAATTGTTTGAGAGAATGCCTTTCGAGTTAAAGAATGTGAAGGC TTGGTCTGTGATGCTTGGAGTTTACGTTAATAATCGAAAAATGGAGGATGCAAGGAAGTTTTTCGAGGATATACCGGAGA AGAACGCCTTTGTATGGTCATTGATGATGTCTGGGTATTTTAGGATAGGTGATGTGCATGAAGCTAGAGCTATTTTTTAT CGAGTTTTTGCTCGTGATTTGGTGATTTGGAACACTTTGATTGCTGGTTATGCACAGAATGGGTATTCTGATGATGCTAT TGATGCTTTCTTTAATATGCAAGGAGAAGGGTATGAACCGGATGCGGTAACGGTTTCTAGTATTTTATCTGCTTGTGCTC AATCTGGTCGTCTTGATGTTGGCAGAGAAGTTCACTCTCTGATTAATCATAGAGGAATTGAGCTTAACCAGTTTGTTTCA AATGCTTTGATTGATATGTATGCGAAATGTGGGGATCTAGAGAATGCGACATCCGTGTTTGAGTCGATAAGTGTGAGAAG TGTAGCATGTTGTAACTCTATGATCTCGTGTCTAGCCATACATGGGAAAGGCAAAGAAGCTTTAGAGATGTTCAGCACGA TGGAGAGCTTAGATTTAAAGCCTGACGAGATCACTTTTATAGCGGTTCTCACTGCTTGTGTCCATGGAGGCTTTCTAATG GAAGGTCTAAAGATATTCTCGGAGATGAAAACTCAAGACGTGAAACCGAATGTAAAGCATTTTGGATGTTTGATACATCT CTTGGGACGCTCCGGGAAATTGAAAGAAGCTTACAGATTGGTAAAAGAAATGCATGTGAAACCGAACGATACAGTATTGG GAGCTTTGCTTGGTGCATGTAAGGTTCATATGGATACAGAAATGGCTGAACAAGTGATGAAGATCATCGAGACTGCTGGA AGCATCACTAACAGTTACAGCGAGAACCATCTTGCATCGATATCGAACTTGTATGCACACACCGAAAGATGGCAAACAGC TGAAGCGTTGAGAGTAGAAATGGAGAAAAGAGGTCTTGAGAAATCCCCAGGGCTTAGCTCACTAGTTTTGACCTGAGTCT ATACCACTTAGCTTTATTTGAAGAAGTAAAATACTTAGTGATTATGAAATTGCCTTTATAAACAGTGTTTATACAAAAAA TCCTAAAATGGGATT ................................................................................ ........................N....................................N.................. ................................................................................ ......................N....i.......................N...N......N.....N........... ......................i................................N..............N......N.. ...N...................................................................N........ ....N..............................................N..................N......... ..........N.............................i......N................................ .............N.........N..i........................N.....N...................... .................................................N........N...........N..N...... ..N.............i...............N........N...................................... ...............N................................................................ ......i............N.....................N.........................i...........N ...............................................................N.............i.. ........................N........................N...............i.............. .................................................i...N.......................... ................N............i...........i..............N....................... ....................................................N...............i........... ...................i............................................................ ...........................................N.................................... .......N....... Pos Score Pred ------------------------ 105 0.354 - 142 0.130 - 242 0.239 - 266 0.272 - 343 0.084 - 348 0.744 Yes 372 0.492 - 376 0.344 - 383 0.152 - 389 0.462 - 423 0.580 Yes 456 0.388 - 471 0.429 - 478 0.228 - 484 0.111 - 552 0.184 - 565 0.195 - 612 0.415 - 631 0.173 - 651 0.320 - 681 0.711 Yes 688 0.209 - 734 0.337 - 744 0.323 - 747 0.717 Yes 772 0.146 - 778 0.271 - 850 0.096 - 859 0.165 - 871 0.066 - 874 0.152 - 883 0.158 - 897 0.794 Yes 913 0.099 - 922 0.218 - 976 0.258 - 1042 0.175 - 1056 0.544 Yes 1060 0.107 - 1067 0.343 - 1084 0.300 - 1127 0.714 Yes 1140 0.195 - 1162 0.122 - 1188 0.690 Yes 1200 0.442 - 1264 0.172 - 1278 0.677 Yes 1305 0.320 - 1330 0.303 - 1346 0.522 Yes 1410 0.782 Yes 1414 0.098 - 1457 0.305 - 1470 0.664 Yes 1482 0.763 Yes 1497 0.381 - 1573 0.155 - 1589 0.687 Yes 1620 0.674 Yes 1724 0.087 - 1768 0.087 -

Explain the output -- Processing locus 374: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGGATTCGAATATAATATGAGCTCTAAATTGATTTTATGTTTGCAACTTTGTTTTGTTTATGTTTTTTTTTAATTGGTTT GTGAATTTTGATTATAACTAAATTTATGAATTTTGCATGGTTGTGAATTACTATATTGCAGGTTTTTGGAAGCTAACTTA TTGTTGGAGGTGGAGAGATATGGCTGACTTTGATGAATATATGGCTGATGAGTATGACATGGATGATCTCGAGGATGATA TGAATGCCGCGTTTGATGGAAGAGATATCGATGCTTCTGATTCCGAAGTCGAAGATTTCGATCAATTGAACAAGGCTGCA GATACTTCTGCTGCTCAAGCACGGAATGGGAAGGATATTCAGGGTATACCGTGGGATAGTCTTACTCTTACTAGAAAAGA TTACAGAAAAACTAGGCTAGAACAATATAAAAACTATGAAAATATACCCAATTCTGGTGATGAATCTGCCAAGAAATGCA TGAATACTGAGAAAGGCAGCTCTTTCTATGAGTTCAGAAGGAATTCTAGATCCGTGAGATCAACGATTCTCCATTTTCAG TTGAGGAATTTGGTCTGGGCTACCTCAAAACACGATGTTTATTTATTGTCCAACTACTCCATCAGCCACTGGTCTTCTTT GACAGGTTGTAGGAGTGAAATTCTTAACGTTAAAGGTCATGTGGCTCCGTCTGAGAAACATCCCGAGAGTTTGTTGGAAG GATTTACAGAGACTCAAGTTAGCACTCTTGCAGTAAAAGAAAGGCTACTAGTTGCTGGTGGATTCCAAGGAGAACTCATT TGCAAGCATCTCGATAGACCTGGTGTGAGCTTCTGCTCACGCACAACTTACACCGAAAACGCTATCACCAATGCAGTAGA TATATATAGAAACTCCAGCGGCGCACTGCATTTCATGGCCTCAAATAATGACTGTGGAGTCAGAGACTTTGACATGGAGA GATATAAGCTTGTCCAACTCTTCCGTTATCTATGGCCCGTCAATCATAGTTCCCTAAGTCCTGATGGAAAACTAGTAGCA GTCGTTGGAGATGACCCAGATGGTCTACTGGTAGACACAAGTAACGGACAGACGATCGGGACGCTAAAAGGTCACTTAGA TTATTCATTTGCATCAGCTTGGCATCCAAACGGTGTAACATTTGCCACAGGAAACCAAGACAAGACCTGTCGTATTTGGG ACACAAGGAAACTCTCGGAATCAGTCGCTGTCCTAAAAGGTAACCTTGGAGCCGTACGGTCCATTAGGTTCACATCTGAC GGACGGTACGTTGCCATGGCCGAGCCAGCAGATTTCGTTCATATCTATGACACAAAATCAGGATACAAAAAGGAGCAAGA GATTGATTTCTTTGGAGAGATTTCTGGGATATCGTTTAGCCCAGATACTGAATCTCTCTTCATCGGTGTTTGGGACAGGA CTTATGGAAGTCTCTTGGAGTATGGTCGAACCAGAGACTACACTTATCTCGATTCATTACTTTGATGGAAATTTAAGCTA AGCCTAAAGAAAAAAAACTTACGTAAAAATAATTTGTTATACGCCGTCTTGGTTAGGTTTCATAATCATATCGACAAAAG AAAA ................N...................N......................N.................... .........................N..........i........................................... ...................i............N.......i......N.....N....i...N...........N....i ...N...........N..............N................................................. .........................N...................................................... ...................................N.......................N...............N...N ...........................N.................................................... ..................................N............................................. ......................................N......................................... ................................................................................ ......................................................................N......... ..................................N............i.........................i...... ...............................N...............................N................ ..........N........N............................................................ ................................................................................ ................................................................................ ...............i..............................N................................. ................................................................................ ...N.................N..........................................N............... ................................................................................ .... Pos Score Pred ------------------------ 17 0.163 - 37 0.088 - 60 0.250 - 106 0.202 - 117 0.724 Yes 180 0.849 Yes 193 0.083 - 201 0.855 Yes 208 0.145 - 214 0.124 - 219 0.864 Yes 223 0.085 - 235 0.075 - 240 0.776 Yes 244 0.144 - 256 0.105 - 271 0.170 - 346 0.223 - 436 0.267 - 460 0.210 - 476 0.360 - 480 0.371 - 508 0.203 - 595 0.469 - 679 0.187 - 871 0.343 - 915 0.411 - 928 0.603 Yes 954 0.730 Yes 992 0.431 - 1024 0.329 - 1051 0.480 - 1060 0.370 - 1296 0.777 Yes 1327 0.130 - 1444 0.431 - 1462 0.399 - 1505 0.119 -

Explain the output -- Processing locus 375: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGGTTTTTAATACAATTCGCCTCTACTTTAGTAACTAACTATTTCTAAACGTCTATATGTATAAAAAGTCAGACACATT TTCTACCAAATCATTTTGTGAAATTTGAGTCTCTCAATGGCATTTAAGAACAATAACAATACCAAACTAACCAAAATTAT CAGTCACATCTCTCAAAACCTATCATTATCTACCTTATGGAAATCCTTAACAGTTTCCGGTGCTCTACTTTTCTTGTTAC ACACATTTGCATTCAACAATCCTCATAAAATGATGACCCATAACCTTTTTGCGCCTCTTGACCTTAGTTTCTCCTCCTCT TCATCTTCCTCCTCCTCGTCAGTATCATTGTCCTTGCAAATAACAAGAGAGCCTCCAACAAATATCAGCCATCTATTTTT TGTCATTGTTGGTAGTACAAAGACTTGGAGATATAGGAGAGGCTACATAGAGCCTTGGTGGAGACCAAACATTACAAAAG GATATGTCTTCCTCGAAAGACCACCTGGCCCTGATCTCTTGCCTTGGCCTCAACAATCTCCTCCCTTCTCTGTCAACAAA GAGTCATTCATCACAAACAAATTCAAGACTCAGATCCGCCTTTTTTATTCGCTTCAAGAGTCTTTTAAGAAGGCGTCCAA AGAGACAAGATGGTTTGTGATAGGAGATGATGATACTCTCTTCTTCTTGGACAATTTGGTGAAAGCTCTCGACCGATATA ACCACAAGAAGCACTATTACGTCGGAATGAACTCCGAAAACGTTTGGTCCAACGCGATTTTCGCTTTTGACATGGGATAT GGAGGCGGCGGATACGCATTGAGCTATCCAACCGTAGTAACACTTCTCAGCAACATGGAAGAATGTATCAAAAGATACCT TGGAGTTTACAGCGATCTTCTCTCATTTCGTTGCTTGGCCGACTTGGGAATTGATCTTACTTTAGAGAAAGGCATGCACC AGAATGATCTACACGGTGACATATCAGGTCTGTTATCAGCTCATCCTCAGTCTCCTCTTATCAGCCTCCACCATTTCGAT GTAATAGACCCGATATTCCCAGGCATGAACCGCCAGCAATCCGTGAACCACCTCATGGAAACCGCGAAAACCGACCAGTC CCGTGTCTTGCAACAAACAATATGTTACCAAAGAGGATATAACTGGTCAGTCTCTGTCTCTTGGGGCTACTCAGTCCACA TTTACCAATCTATATACCCAAGAAGCCACCTGAAACGCCCTCTCGAAACATTCCGACCATGGAAGGATGTTAGAATACCG GCGTATGGATTTAACACTAGACGGGTGACGAATGATCCTTGCGAAATGCCTCGTCAGTTTTTCTTTGATTCAGTTGTGGA AGACAAGAACCAAAGCTTGGTAACTACCATCTACAAGATGAAAATGGAAAGGCGTTTGCCTCCTTGTTTGCTAAATGGCA ACCATTCTTCTCGTAACATTACACAAGTCCGAGTCATTGCTACTACTATGCACAAGATGGTTGAGGGAATCGAATGTTGC GATGTGCAAAATGTTAACAGTACGGAGATTTTGGAAGTGAAGATAAGAGATTGTCATATAGATGAAGCTCTTGCTTAGAC TAAGTCTTATGATACATGTAATCATCAACTATGATTGATCTGAAAATCTTTTTCATTTGTATAACAACAGCATCTGTCAT TTATTGATATGCTGTAC .........................................................N...................... ....................................N........................................... ....................................N........................................... .............................i..N............................................... ................................................................................ ................................................................................ ...i............................................................................ ................................................................................ .........i................N..N.................................................. ..........................i............................................i......N. ......................................................N.......i................. .........................................................................N...... ...N..........................................................................i. ........................i.............................N......................... .....................i.......................................................... ..........................................................N.......N............. ....i..........................N.............i.................................. .....................................i.....i..............................N..... ...............................................N........i................N...... ........N......N..............N................................................. ........N........ Pos Score Pred ------------------------ 58 0.390 - 117 0.364 - 197 0.258 - 270 0.558 Yes 273 0.483 - 484 0.503 Yes 650 0.628 Yes 667 0.325 - 670 0.301 - 747 0.739 Yes 792 0.520 Yes 799 0.440 - 855 0.437 - 863 0.537 Yes 954 0.347 - 964 0.390 - 1039 0.502 Yes 1065 0.501 Yes 1095 0.421 - 1142 0.508 Yes 1259 0.341 - 1267 0.352 - 1285 0.534 Yes 1312 0.393 - 1326 0.556 Yes 1398 0.737 Yes 1404 0.700 Yes 1435 0.192 - 1488 0.285 - 1497 0.603 Yes 1514 0.228 - 1522 0.120 - 1531 0.170 - 1582 0.195 - 1609 0.080 - 1616 0.138 - 1631 0.269 - 1689 0.044 -

Explain the output -- Processing locus 376: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCTAGGACATGTCCATAAAGGAACAACACCTTAAACCTTCTTTCCTGTTGGGTCTTTGTTTAAAAATGATAAAATTATTT TGGTTGTGGGCCTTAGGTTGAAAAAATAAGATGGGCTTTCGTTATTTTAGGCCCATAATGAAACTAAAACTAATTGTAAA TTCAAATACTTCTCTCCGCACCTTTTCATCAATTAGGAAAGAGAGAGAGAAGAAGGAGCGTTGTTGTCTCTCACACTCGC ATCGAGAAAGTACAAACTCTTCGCCGAAGATGACGAAGAAGCCAAATCTCCGATCAAAGGATTCATCTCTCGGAGACGAT GCTGAGAAACTCTACGAGAAGAAGATTCTGGATCTGGAGTCCGTGAACGAAGCTCTCAAGAGTGATGTGGAAGAGCTAAG GTCAAAATTGGCTGATGTTTCGATAAGCTCATCGGTTGGTACGTTACAGTCAAGCAGAAAGTTTTCTCAAAAGTCTATTG CTAACAAAGAGGAGGGTATGAGTTCAAGGAGTAAGTCTATGTGCTCCACAAAGAAGTATAAAACTGAGAGTTCTGTTAAG CAATTTGATGGTGAAGTTCAGAAATTGAAGGCGCAAAAGGTTAAGTTGCATTGCAAGATCAAGCTAGACTCTATGCAGTT CAGACTATTGAAAGCTTCACTAGAAAAGGAAGTTCTTCAGCTCAAAAAAGAGCTAAGGAAAAGTGAGTTTGAGAAACATG TCCTGTCAGCTCTGAACAATCGCCAGAAGCTGATTTTGCAGCTGAAGAATACACAAGCTTTGACAGCCCTGAAGCGTCTA AAAATGCTGCTTCAATCTAAAAAGATTTCTTCCAACAAAAAGAAAGGCCCATCAAAAGGAACAAGCTCCGAGATTCAGGA GTCTAGCAATGAATCAGGCCTACTAATGAAACTGAATAAAATTCATTCTGATTACGAACGCCAAATGAAAGAAATGGCTG AAGAGATCAAAAGGTTCAGCTTAGAGGCAGGTTTGTTGAAGGCAGAATTTGAGGGAGAGCAAAGCTCATGTTCTGCGTCT TGTGACAACCAAATCAACCACACTCCAATGGACTCAGAACTAAAGGAACTCAAAGAGGAATTTAACAAACTGAGTACTCT TGTCAGCCAGATGGAAATGACCAAATCACAATTCAGTGAAACTGATAAAGTTCAGAGCGAACCAGTGGAACGCTCTATCA CCAGCAAGAATATCGACGATGAATCAAACTCGGAGCCATTTCAACCGGAAACTTCTGAGGAAACACTTTGCAAAAAGGAA CAGAGCAAAGCAGAAGTGTGTTGCTCTTGCACTAAAAAATCTTTGTGCAAGACCAAAAGTTGCAAATGCAAGGCAAATGG AAGTGGATGTGGGGACTCTTGTGGCTGCTTAGCTTCCAAATGTAGCAACAGAGACGAAAGTGCGAAACCCGACAAACCAA TGGAACCAATAGATGTTAAGAAGCCTGCTGGAATTAGCCATGACGACAAAGGCGCAAATAAACAACCATTACGTGACATA GGGAATATACAAGAAGCTGTAAAAGTTGGTAAGTTGAGGAAAGTGCAGAAACGGGTTGCTAAGAAATAATGAAAATTGAT AAAAGGATATGAAAGTAAACAGAGAAACACTTTAAGAAGCTTAAACGTTTTTCTTTTGGTTTATATACCAAAACCGCTGC AATCATTA ........N........................................................N.............. ..............................i..........................N...................... ................................................................................ .............................i................................................N. ................................................................N............... ..............N................................................................. .................i....................N......................................... .......N................................................................N....... .............................................................................N.. ................................................................................ ...N............................................................................ ........N................i......................................N........i...... ...................................................................N............ ...........................N.................................................... ..........N.....i............................................................... ..................N............................................................. .................................................................N..........i... ......N................................N.......................................i ............N..........................N........................................ ....................................................................N........... ........N....................................................................... ........ Pos Score Pred ------------------------ 9 0.361 - 66 0.202 - 111 0.704 Yes 138 0.203 - 270 0.741 Yes 319 0.118 - 385 0.426 - 415 0.159 - 498 0.762 Yes 519 0.318 - 568 0.197 - 633 0.286 - 718 0.484 - 804 0.471 - 889 0.319 - 906 0.585 Yes 945 0.470 - 954 0.831 Yes 1028 0.384 - 1068 0.319 - 1131 0.401 - 1137 0.654 Yes 1219 0.247 - 1346 0.292 - 1357 0.561 Yes 1367 0.424 - 1400 0.169 - 1440 0.570 Yes 1453 0.445 - 1480 0.335 - 1589 0.360 - 1609 0.103 -

Explain the output -- Processing locus 377: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCCGTCTTTTCTCTTGTGATAATTATCCAGAATATTCTCACAATTAAATTGATTTTTTTGTAACCGTGAAACCTAATTT ACCTAAGGCTCTTTTTGTTAGTTAAGGATATATTTCTCATATATGGTTGTTAGAATATTCACTGTATATCCTCTGTATAA GTTACGACCATATATGAGAAAACTTTTCTTCTTCCTTTGCACACACGTCCCAAAACAGAGAGCAAAATTCACCAGAATCA TGGAGAGCTGCCAGAAGAAACAAAAATTGGAGACACCAACAGAGCTCAGTGATATGCCTGATGACTTAATCTTCAAAATA TTCTCGTTCCTTCCGTTTTTTAAAGAGGATTTAGCAACACGTTTCATATCAGAATATGGGAAGGGTCTTTGGAATCCAGA CCCGAATGCCATATTTGATGATGAATCACTCGATATGACTTGTATGAGTTTTGTATATGGATCTTTGATGTCCAAGGATG CTCAGATTCTAGATAGCTTGCATCTCAAGCTTAGGAAATACTATATTGCTTCAGATATCAATTTTCTTGTTCAACTTGGG GTTAATCGATCTATGAGAGAGCTGAGAATCGACTTCTTTGGAAAAACCCTAGAATTACCGTGTTGCTTGAGTACCTGCAC AACCCTAAAAGTTTTGGTACTCGATCATTTAAATATCATGTCTGTCCCGGGTTGGTTTCGTTTGCCATCAGTCGAAACTC TCCAGCTTTCATCGGTTACCGGGGGCAGCAACTATGTTCCAAGTCTTATAAGATTATGCTCTGTTCATGAACGTTTGGTT GTAGACCAAAACAGAAATAAAGATCTGGTTAATATCAATGTGCCTACTTTGAGGAGCTTATCTATCGATAACAAGAGAAG GGGACATGTGCCACTCGGTAGCTTTTGGATAAATGTTCCTTCTTTGATGTTTTTGAACATTAAGGATACATTCCAAACAA TATTTGAATCTATGCCTGCGATGATCAAAGCGAATATCGAGGTTGCTCATGACCAATCGCAATCTGTGAAGTTCATAGAA TCTCTTACCTCAACCCGACATCTTTGTCTTTGTTCTCCAACTTCAGAGAATCCATATCCTAATGGCACAAAGTTCTCCAA TCTTGTACATCTAAAGCTATGTACATGTTCTGCGGGATGGCAGAATCTACTTGCGTGTATGCTCAATGACGCTCCCAATC TTCGTTCTCTTACTCTTAAGTTGAGACAAAAATCCGATGTCAATCCGAAGAAAGTCTGGGAAAAGCCAACAGTCGTTCCT GAATGTTTATCTACGCGTCTTGAGATCTTGAAATGGAGAGATTATGAAGGCACTGAGCATGAGAAGGATATGGTTGGGTA CATACTAGCAAACGCTACTTTTCTACAGAGGGCAACATTCTCAACGAAAGATAGAAACCAATGTGATTCCAGGTTCAGCG AGTTACAGTCTATGGAGAGAGTTTCAGAGATATGTGAGTTTGTGTTTGACTAATGTGACTCTCTTATGTGATGGTGTGTG TCTCTCTAGAAGACGCAAAGACGTTCTTAGCTTTCTTCTTCCTTTCCCTTAGACTTTTATATTTGGTGTTTT ................................................................................ ..........................................i..................................... .............N.................................................................N .....................................................N......N................... .......................................................N........................ .....N...........N..N.............i........N............N..........i.........N.. ................................................................................ ............N................................................................... .....................................N.......................................... .................................N.....................N..........N............. .....................................N.......................................... .....N..........................N.............N................................. ...........N........N...........................N............................... .............................................................N.................. ..................N.....N...........i.....................i......N.............. ....................................N........................................... ..N.............................i..........N..............N..........N.......... ............................................................N................... ...........N...................N....................N............N....N......... ........................................................................ Pos Score Pred ------------------------ 123 0.586 Yes 174 0.281 - 240 0.180 - 294 0.400 - 301 0.303 - 376 0.410 - 406 0.121 - 418 0.162 - 421 0.235 - 435 0.560 Yes 444 0.350 - 457 0.091 - 468 0.533 Yes 478 0.206 - 573 0.167 - 678 0.465 - 754 0.165 - 776 0.340 - 787 0.175 - 838 0.147 - 886 0.148 - 913 0.244 - 927 0.293 - 972 0.352 - 981 0.322 - 1009 0.379 - 1102 0.375 - 1139 0.499 - 1145 0.423 - 1157 0.606 Yes 1179 0.551 Yes 1186 0.309 - 1237 0.451 - 1283 0.184 - 1313 0.560 Yes 1324 0.227 - 1339 0.337 - 1350 0.374 - 1421 0.442 - 1452 0.293 - 1472 0.202 - 1493 0.102 - 1506 0.091 - 1511 0.089 -

Explain the output -- Processing locus 378: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATGTGATCGATATTATTTTTTTAACAGTATAAAACCACTTAACACTTATTTAGTTTGTTGACCTCGTGGCTTCATATTG ACTACAGAATGAAATAGGTAAACTATAAAGTCTACACTATTTATGGCTGAAATTTATTTGAATACATATCCACCACTTGT CTTGTTCTTGAAACACACGGTCGTGGCCATGTCTGTTCTGCTTCCGATGTATCCTCTCCAATCCTTGGCCAGAAGCAAGA GATTGTTTCCGATTCGAACCAGATCAAAACCGGTTTGTTTAGCACTGACCAATGCTCAAACAAACGGTGGTGACCAAGAG GAGAAAGTAGTGATTGTTGGCGCCGGGATTGGCGGACTTGCCACTGCCGTTTCACTTCACAGGCTTGGAATCCGGTCGGT GGTGTTGGAGCAGGCAGAGTCGCTTCGTACTGGTGGAACATCACTGACGCTATTCAAGAATGGGTGGCGCGTTCTTGACG CCATTTCCGTCGGGCCTCAACTCCGAACGCAGTTTCTTGAAATCGAAGGGATGGTAGTGAAGAAGGAAGATGGAAGAGAG CTTCGTTCTTTCAAATTCAAAGACGATGATCAAAGCCAAGAAGTTCGGGCCGTGGAGAGGCGAGTACTCTTGGAAACACT TGCAAGCCAGCTTCCTCCACAAACCATTCGGTTTTCCTCAAAACTGGAAAGTATACAAAGCAATGCCAATGGTGACACTC TCCTTCAACTTGGAGATGGAACCCGGTTGCTTGCACAGATTGTTATTGGTTGTGACGGTATCCGGTCTAAGGTTGCAACT TGGATGGGGTTCAGTGAGCCGAAATATGTTGGCCATTGCGCTTACCGTGGTCTTGGGTTCTATCCAAATGGACAGCCATT TCAGAAAAAGGTGAACTACATATACGGTAAAGGGATTAGAGCTGGATATGTACCTGTATCTACAACAAAAGTCTACTGGT TCATCTGTTTCAACAGCCCATCTCTAGGGCCCAAAATTACGGATCCAGCGATCCTAAAAAAACAAGCCAAAGAACTGGTT AGTACATGGCCAGAAGATCTGCAAAACCTCATCGATTTAACACCTGATGAAACTATCAGCAGAACTCCTCTAGTAGACCG GTGGCTATGGCCTGGCATTGCTCCTCCAGCATCAAAAGGACGAGTGGTTCTTGTTGGAGATGCTTGGCACCCAATGACTC CAAATCTTGGACAAGGTGCTTGTTGTGCCTTGGAAGATTCAGTAGTTCTGGCAAATAAACTTGCCAATGCAATAAATGGA GGAACCGAATCAATTGAAGTAGCAATGGAATCATACGGGAGTGAGAGATGGTCTCGAGCATTCCCGTTGACAGTACGTGC AAACCTAGTTGGCGCACTTCTGCAATGGGAGAATCCTCTAGTGTGTTCCATTAGAAACAATATTGTCATACCAAAGTTAC TGAGGCTTGGACCAATGCTTGAACACACAAACTTTGAGTGTGAACCTCTCTTTGTTTCTAAAAGCTGAATTTCGTATATA TAGTGAGATCCATTGAATAGAGAGACTTGAACAATGTATAATCATAGGAACAATATAAACTCTGTGATTGAATGTAAAAG AATCATA ........i.................................N..................................... ............................N.................N................................. ...................................................N............................ ................................................................................ ...........................................................i.................... ..................................................N..................i.......... .........................N...................................................... ..............................................................i.....i........... ...............N................................................................ ...N.....................i.........................................N............ ...............................................N................................ ................................................................................ .....i........................................N................................. ......N....................................................N.............i...... ..................................................................N........i.... ........................i......................i................................ ........................i....................................................... ..............N................................................................. .................................N.....................................N........ ....... Pos Score Pred ------------------------ 2 0.263 - 89 0.605 Yes 123 0.466 - 189 0.488 - 207 0.320 - 292 0.365 - 460 0.710 Yes 531 0.481 - 550 0.719 Yes 586 0.124 - 703 0.531 Yes 709 0.590 Yes 736 0.278 - 804 0.447 - 826 0.676 Yes 868 0.227 - 928 0.387 - 1046 0.640 Yes 1087 0.134 - 1127 0.343 - 1180 0.222 - 1194 0.682 Yes 1267 0.356 - 1276 0.720 Yes 1305 0.643 Yes 1328 0.585 Yes 1385 0.589 Yes 1455 0.365 - 1554 0.138 - 1592 0.040 -

Explain the output -- Processing locus 379: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCAGGGTCATTTCCATTTTTTTATCAAAATTGTACAATTTCTTTTATTTTATTCGTATTTGAGTGGTTTCATGTATCATG TATTATATAATATTTTTATAATATAATATAATTGATTCGGTGTTAAAAAAAAAAAAACATATTAAACGGAAATTGCGAAC GTTAATGGAAAAGAATATATTTTATTATTATTATAAATGCTGTTTGGTACAGGTGGCTTACAAAAATCCAAAGTGCCGTA ACTTATTAGCTTCTCTTTCATTTCAAGTCAAAAAGTCCAAACCTCTGCAGATTTTATTCCTAAACCCGACCATGGAAGAA TCATCAAACCAACAAATCCTCAACTCCGAGACCCGACCCGACCCGATTCAAAAACCACCGGAATCACCAGCCACCAAACT CTCATTCCCGAGACTCTTCCTCTGGTTTGATCAATCAAATCGCATCAAAACTCTGCTCTCATGGTCCATCTTCTTCCTCC TCGCCGTCATCGTCCCAATGATCTCACACTTCGTGCTAATCTGCGCCGATTGCGATTTCAAACACCGTCGTCCTTACGAC GGTTTAGTGCAGCTTTCGTTATCGATCTTCGCCGGAATCTCGTTCGTTAGTCTCTCAGATTGGTCCAAGAAATATGGAAT CCGAAGGTTTCTCTTCTTCGATAAGCTTAAAGATGTTAGCGATAAAGTTAGGATCGGATACGAAGCTAAAATCCAGAGAT CAATGAAGCTACTAGCTATCTTTGTCCTTCCATCGACAACGCTTCAAGCTATCTATCGCATTTGGTGGTATGCTTCAGGC TTTAACCAAATCCCTTACATTATAAACCCAACGCTGAGCCATGTCTTAGCCTGCACACTCCAGCTCTCTTCTTGGCTCTA CCGTACATCACTCTTCATCATTGCTTGTATCCTCTACCAAAACATCTGCCACCTCCAGGTCCTCCGTCTTGATGAATTCG CACGCTGCTTCGCCTCTGAGATCAAAGATTTCAGTTCCATACTCGCCGAGCATCTCAAAATCCGTCGTGAACTGAAGATT GTCAGTCACCGGTTCAGACGATTCATTCTTTTGTCATTGTTTTTCGTCACTGCCACTCAGTTCATGGCATTGCTGACTAC CATCAGAGCTAGTGTTCCCTTTAATATCTACGAAGTTGGCGAGCTCGCGTTATGCTCCACAAGCTTGGTGTCAGGACTAT TCATATGCTTGAAAAGTGCAACACAAATGACTCACAAAGCTCAATCAGTAACGAGCATCGCCACAAAGTGGAACGTGTGC GCGTCTTTAGATACATTTGATGTTCTTTATGACGGAGAGACTCCTAAATGTCCAACAACTACACAACATTCCCAGATTTT ATCGCGTCGTCGTAATGTTGTTCAATCGTCTGATGATGATGAAGAAGGAGAAGGAGATGACAATGATCTTGAGATACATC CAATCTTTGCCCGCGCCATTTCTTCTCAGAAACGTCAAGCTCTAGTGACCTATCTAGAGAACAACAGAGCAGGGATCACT GTTTACGGATTCTTGGTGGATAAAACATGGTTGCGTATGATCTTCAGCATCGAACTTGCTCTTCTTCTATGGCTGCTCAA AAAGACAATCATGAACATAACATGAAGACAGAATCAGTGTCACACAACACACACGAAGAGATTGGTACTAAGATGTTTTT ATTTCAACAATGGCGATATAACAGATTCTTTTTGTAGATCTTCT ......................................................................N......N.. ................................................................................ ....N...............................N........................................... .......................................................................i........ ................................................................................ ............................................................i................... .................N.............................................................. .........................................................................i...... ................................N............................................... ..N..................................................................i.......... ........................................i....................................... .......................................................................N........ ................................................................................ ...............................................................N................ ...................................................i............................ ....i.....................i..................................................... ...................N........N..................N................................ ..............N.................N..N..N.................N.....N................. ................................................................................ ..........................i.........N...............................i........... ..........N..........N..................................................N....... .........N.................................. Pos Score Pred ------------------------ 71 0.329 - 78 0.252 - 165 0.154 - 197 0.321 - 312 0.588 Yes 461 0.704 Yes 498 0.429 - 634 0.509 Yes 673 0.435 - 723 0.327 - 790 0.533 Yes 841 0.753 Yes 952 0.357 - 1104 0.403 - 1172 0.721 Yes 1205 0.672 Yes 1227 0.658 Yes 1300 0.270 - 1309 0.337 - 1328 0.327 - 1375 0.290 - 1393 0.278 - 1396 0.142 - 1399 0.389 - 1417 0.284 - 1423 0.147 - 1547 0.574 Yes 1557 0.309 - 1589 0.660 Yes 1611 0.175 - 1622 0.338 - 1673 0.055 - 1690 0.094 -

Explain the output -- Processing locus 380: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTCTTGGCTTCATGTTTTATCGACGTCACCATACATTTATTTCCCTTATAGACCCACTTCTCCTCCCCAAAATAAATAC TATAATTAGAGAAAGCATTAAACCCACCCACCCAAAACCATTAAATCATTAAGAAGAAAATGTTTATTAATAATAAGAAA AATGAAATTATACACAACTAAAAAAAAACAAAAGGAAGAAGAAACATCACAATCACCAATCACCTCACTATATCAAATTC TTTCCTTATTCACTAACCAAAAAATAAATAAACTCAAAACCATTCTTCATTTCGCCGACACTTTCTTCTCCACCACCACC ATGGATGATCTCTCCAAGCTTCTCTTCTTCCTCCTCCTCACTATCTCCATTACCACCGCATTACCCGATAAACCCGGGTC GGGTCAAATAAACTCAAACTCCGTCCTCGTTGCTCTTCTTGACTCACACTACACAGAACTAGCAGAGCTCGTCGAAAAAG CTCTTCTTCTCCAAACCTTAGAAGAAGCTGTTGGTCAACACAACATCACAATCTTTGCTCCTCGTAACGATGCCTTAGAA AAAAATCTTGATCCTGAATTCAAATCTTTCTTACTCCAACCGAAAAACCTCAAATCATTACAATCTTTATTAATGTTTCA CATTCTTCCCAAAAGAATCACTTCTCCACAATTCTCCTCCGCCGTCGTTAGCCACCGTACTCTCTCCAACGACCACCTCC ATTTCACCAACGGAAAAGTCAACTCCGCTGAAATAACCAAACCCGATGATTTAACTCGTCCCGATGGAATCATCCACGGA ATCGAACGTCTTTTAATCCCACGTTCTGTTCAAGAAGATTTCAACCGCCGTCGTAGTCTCCGTTCAATCGCTGCCGTATT ACCGGAAGGAGCACCGGAGGTTGATCCAAGAACTCACCGTCTCAAGAAAAAGCCAGCACCAATTCCCGCCGGAGCTCCAC CGGTTCTTCCGGTTTACGACGCAATGTCACCTGGTCCGTCACTAGCTCCGGCTCCGGCACCAGGACCGGGTGGGCCACGC CACCATTTCAACGGAGAGGCTCAAGTTAAAGATTTTATCCACACGCTTCTTCATTACGGTGGCTACAATGAAATGGCAGA TATTCTCGTTAATCTCACTTCTTTAGCCACCGAGATGGGTCGGCTCGTGTCGGAAGGTTACGTTTTAACCGTTTTAGCAC CAAACGACGAAGCTATGGCTAAATTGACCACGGACCAATTGAGCGAACCGGGAGCTCCAGAGCAAATTATGTATTATCAC ATCATACCGGAATACCAAACCGAAGAGAGTATGTACAATTCTGTACGCCGGTTTGGGAAAATCCGGTACGATTCACTCCG GTTTCCTCATAAAGTAGAGGCTCAGGAGGCAGATGGTTCGGTTAAGTTCGGTCATGGTGATGGTTCGGCTTATTTGTTCG ATCCTGATATTTATACCGATGGACGGATTTCGGTTCAAGGGATTGACGGTGTTTTGTTCCCGGAGGAAAAAACTCCGGTC GAGAAGAAAACCGGCGTTCCAGTTGTCAAGAAAGCACCTAAACCAAGAAGAGGTAAATTGATGGAAGTAGCATGTACAAT GCTGGGATCACAATTTCCAACATGTCAATGAGATCAAATTAAATCCGGGACATGAGAATTTTATAATTCTGTAAACATGT GATAAAAATGTGAACAGAAAAAAGAAATAATGTAAATGATTATTTTTTTG ............N................................................................... ...........................................................N.................... ................................................................................ i...N........................................................................... ................................................................................ .....................................................................N.......... ........................................................................i....... ................................................................................ .............................................N.................N................ ................................................................................ ................................................................................ .......................i........................................................ ...................................................................i....i....... ..................................i............................................. ..............N.....................................................N........... ..............................i................................................. ................................N....................N.....N.................... ..................N............................................................. ............................................................N..........N......N. .....................i.....N.......................N........................N... .......N.....................N.....N.............. Pos Score Pred ------------------------ 13 0.367 - 140 0.495 - 162 0.442 - 321 0.714 Yes 325 0.385 - 550 0.331 - 633 0.695 Yes 766 0.496 - 784 0.458 - 984 0.808 Yes 1108 0.502 Yes 1113 0.901 Yes 1155 0.900 Yes 1215 0.444 - 1269 0.298 - 1311 0.504 Yes 1393 0.356 - 1414 0.373 - 1420 0.123 - 1459 0.205 - 1581 0.273 - 1592 0.395 - 1599 0.368 - 1622 0.510 Yes 1628 0.160 - 1652 0.064 - 1677 0.057 - 1688 0.070 - 1710 0.078 - 1716 0.033 -

Explain the output -- Processing locus 381: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAGTTTTATAAATTTCATTGGGTGCACCCCTTAAGGCCTTAACACAAGTGTAGATACGCCCCTGGAGTCAGTTGTCCAG TAGTATGTCACGTTCTTCTAGACAGAGTTTTTTTCTAATGTAATAAACAAAGCATGGTTGGTCACCCTACAGATACATTA TATTTACGGATTCTTGATCATGATTAATCTATCTCTTAACCTTGGAGAAACAATGATAGCAATCATCGTTGAGTTCCAAA ACTTCTTTATCTTCATCCTTCTATGCCTCTTCTCACTCCTCTGTCACTCTCTCTTCTTCAAGAAACCAAAAGACTCACGA AGCTTTGTTCTGCCTTCAAGCCCTCCTTCTCTTCCAATCATCGGCCATCTTCACCTTCTCCTCTCTGTCCTAACTCACAA GTCTCTTCAGAAACTCTCCTCCAAGTACGGACCTCTTCTCCTTATCCGCATCTTCTATGTACCCATCATTCTTGTCTCCT CTTCCTCTATGGCCTACGAGATCTTCAAGGCCCATGACGTGAACGTCTCTTCTCGCGGTATTATTGCTCTTGATGAGTCT CTCATGTTTGGGGCTTCTGGCATCTTGAACGCTCCCTATGGAGATTACTGGAAGTTCATGAAGAAGCTCATGGCCACTAA GCTACTCCGACCGCAAGTGCTGGAGCGGTCACGAGGTGTTCGTGTTGAAGAGCTACATAGATTTTACAGGAGCATTCTTG ATAAGGCGACGAAGAATGAGAGCGTTGAGATCGGTAAGGAAGCGATGAAGCTCATGAACAACACCTTGTGTAAACTGATC ATGGGAAGGAGTTTTTCAGAGGACAACGGTGAATCAAATAGAGTCAGGGGCTTGGTTGATGAAACCTATGCCTTGTCCGA GAAGATATTCTTGGCAGCTATATTGCGCAGACCTCTTGCAAAACTTCGGATCTCACTATTCAAGAAAGAGATAATGGGTG TCTCCAACAAATTCGATGAGCTACTAGAGAGGATCCTTCAGGAACGCAAAGAGAATTTGGAAGAGAAGAATAATGAAGGT ATGGATATGATGGACGTGTTGTTGGAAGCTTATGGAGATGAAAACGCTGAGTATAAGATCACATGGAAGCATATCAAGGC ATTTTTCGTGGAGTTTTTCATTGGAGGGACTGATACCTCAGTGCAAACAACACAATGGGCAATGGCCGAGATGATCAATA ACGCTAACGTTCTTGAGAGACTGAGAGAAGAAATCGTCTCCGTTGTAGGGGAAACAAGGTTGATCCAAGAAACAGATTTA CCAAACCTACCTTATTTGCAAGCAGTGGTTAAGGAAGTTCTAAGATTGCACCCACCATCACCGGTCTTAATACGGAAGTT CCAAGAAAAATGTGAGGTCAAAGGATTTTACATACCGGAGAAGACAACACTCATTGTTAATGTTTACGCTATAATGAGAG ATTCTGATTCTTGGGAAGATCCTGAGAAATTTAAGCCAGAGAGGTTTCTAACTTCTTCAAGATCAGGGGAAGAGGATGAA AAAGAGCTTAAGTTTCTTCCTTTTGGCAGCGGAAGGAGAGGATGTCCTGGAGCAAACCTTGGTTCTATATTTGTAGGAAC CGCAATAGGAGTGATGGTGCAGTGCTTTGACTGGAAAATCAAAGAAGATAAGGTCAACATGGAAGAGACTTTTGAAGGAA TGACCCTGAAAATGGTTCATCCGCTTACTTGCACTCCGTTCTTCGAACCCAACCTTTATCTTTTGCTTTTAATCTCTAAA TTTCCGTGTCTGATACTTAGCTTTGGAGCTTGTTAACAAGTCTACTTTCTTTTGTTCTATATGTTTGCATTTGCTCTCTT CTATGTTGTCTTTGTATTTGCATTGCTATTTGACGTTTAATATGTTTGCTCGGAA ................................................................................ ....N................................N...............i.......................... ...................N................................N........................... ......................i......................................................... ................................................................................ ........................................................N....................... ........i........................N......................................N....... ...i.................................N...................N...........i.......... ................................................................................ ...............N............................i........N.......................... N.........................................................N........N............ .........................................................................i...... ...............N........................................................N....... N.....N..i.....................N.....N........................i................. ......................................................i......i........N......... ................................................................................ ................................................................................ .........i.................................................i.............N...... ...........................................................................N.... .........................................i...................................... .............N............................................N....................N ...........N.................................................................... ............................................................N................... ..N......................................N............. Pos Score Pred ------------------------ 85 0.115 - 118 0.119 - 134 0.651 Yes 180 0.222 - 213 0.169 - 263 0.533 Yes 457 0.211 - 489 0.784 Yes 514 0.484 - 553 0.285 - 564 0.556 Yes 598 0.237 - 618 0.364 - 630 0.681 Yes 736 0.438 - 765 0.547 Yes 774 0.374 - 801 0.391 - 859 0.129 - 868 0.254 - 954 0.830 Yes 976 0.172 - 1033 0.181 - 1041 0.320 - 1047 0.418 - 1050 0.584 Yes 1072 0.181 - 1078 0.076 - 1103 0.641 Yes 1175 0.531 Yes 1182 0.743 Yes 1191 0.384 - 1370 0.502 Yes 1420 0.559 Yes 1434 0.492 - 1516 0.444 - 1562 0.648 Yes 1614 0.340 - 1659 0.498 - 1680 0.242 - 1692 0.362 - 1821 0.112 - 1843 0.053 - 1882 0.069 -

Explain the output -- Processing locus 382: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATACTTTTATTAATTCTAGTAATTTGATACATCAAAACTAGAACTCTACCACACTCTTCATATGATAGTTAAGTAAAA AACTATTCGCACTTATTATAATACTATTTTGTATTGGTGATCTCACTAATACTAATACACACAACCTCTAGCCTTACAAA AAATAAAAATAAAAATCTCTTACAGAAGAACAAGTCTGTCTTTTGCAAACATTGTTTGAAGAAAAGAAATCAAATGATGG AACAGAGCAGAAGAACAGAACAAGTTCTGTTAGTGCAGAGATTAAAAAGATTGATAGTCTCCTTTGTTTTTTGTCTTCCC ATGTCTCTTCTTGGTCTCCTTCTCATGCTTCTCTTAATCTATAACAGCTTCTCTGTTTTCTCTCTTCATCTTGTTCCCAA CCAACCTATTCAATCCACTCTTTCACCAACTCATCTTCAGATTCTTCATCACCAGACATCAACATCATCATCTGTGTCAG ATTCTTCATTGTTACTTGTGGTGAAAGAAACCTCTTTAGGGTTCATACAGAAGCAGAACGTTTCGTCGACGAGGATAGAG AAAAAGACGAGGAGATTCAAGAGAAGTACCGAGTTAACTCCTGCGATAACACAGCGGTTACAAGTCAAATCAAGACAAAG ATTCCAGACGAGGGTCAAGTCTTTGTTGTCCAAATCTTCTTGTGAGTCACTCTTCTTCATGACTTGGATCTCCTCCATAG AATCTTTCGGTGATCGAGAGCGATTCACTATAGAAAGTCTCTTCAAATTTCACCCAAATGGCTGTTTGATCTTGGTCTCA AACTCATTTGACTGTGACAGAGGAACCTTAATCTTGAAACCCTTTACAGATAAAGGGCTTAAAGTGCTTCCAATCAAACC TGATTTTGCTTACATCTTCAAAGATACATCAGCAGAGAAATGGTTTGAAAGATTGAAGAAAGGAACACTCAGTCCAGGAG TGATTCCATTAGAGCAAAACCTCTCAAACCTTCTAAGATTAGTCCTGCTCTACAAATACGGCGGAATCTACTTAGACACA GACGTTATAATCCTCAAATCTCTCAGCAATCTCCACAACGTAATCGGAGCACAAACAGTTGACCCGGTTACAAAAAAATG GAGTAGGCTCAACAACGCGGTGCTCATCTTCGACAAGAACCATCCTTTGCTTAAAAGGTTCATCGACGAATTCTCAAGAA CCTTTAACGGTAACAAATGGGGACACAACGGTCCCTACTTAGTGTCGAGAGTCATTACAAGAATCAAGATATCATCCTCT TCAGATTTGGGATTCTCTGTTCTTCCACCATCTGCATTTTATCCGGTGGACTGGACTAGAATCAAAGGATTCTACAGAGC GCCCACGAATGAGAGCGATGCTTGGTTGCGGAAGAGGCTTACGCATTTACGCAAAAATACATTCGCTGTTCATCTTTGGA ACAGAGAGAGTAAGAAACTCAGGATTGAAGAAGGAAGCATCATTCATCAACTCATGTCTCATTCTTGCATCTTTTGTAAC TCTTCTTCTTTACATTTAAGTTAAAACATGTAAAGTAAATTTCCACAAGAGAGATAGAGAGAGAGACAAAAAAAAGGACA AATCTTTGTGTGTTGTGAACATACAGATGTGCTGATCTATCTG ...............................................................N................ ................................................................................ .........................................................................N..N... ................................................................................ N.......................N....................................................... ................................................................................ ................................................................................ ................................................................................ ..........................................................N..................... .........................................................i...................... ................................................................................ .......................................N........................................ ................................................................................ .............................................................................i.. ................................................................................ ................i............................................................... ................................................................................ ........i........i.............................................................. .....................................................N.......................... ...........................N.................................................... ..........................N................ Pos Score Pred ------------------------ 64 0.119 - 234 0.270 - 237 0.494 - 321 0.177 - 345 0.175 - 699 0.221 - 778 0.616 Yes 920 0.456 - 1118 0.713 Yes 1217 0.703 Yes 1369 0.595 Yes 1378 0.634 Yes 1494 0.168 - 1548 0.375 - 1627 0.036 -

Explain the output -- Processing locus 383: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTGAGAATCGCATCCAGATTAGGTTGATACAATTTATCTCGTTTTTTCACTCGAAATTAGATGGTTGTTTGTGGGACT TTCAATATTCAGTCTTGGTAAATTTTGATGATGCAGATTATATATAATTGCGTGAAGAAAAGAGAAATGGTGGGTGGCAA GAAGAAAACCAAGATATGTGACAAAGTGTCACATGAGGAAGATAGGATAAGCCAGTTACCGGAACCTTTGATATCTGAAA TACTTTTTCATCTTTCTACCAAGGACTCTGTCAGAACAAGCGCTTTGTCTACCAAATGGAGATATCTTTGGCAATCGGTT CCTGGATTGGACTTAGACCCCTACGCATCCTCAAATACCAATACAATTGTGAGTTTTGTTGAAAGTTTTTTTGATTCCCA CAGGGATTCATGGATACGCAAACTCCGTTTAGATTTGGGTTATCATCATGATAAGTATGATCTCATGTCATGGATTGATG CTGCGACTACGCGTAGGATTCAGCATCTTGATGTTCATTGTTTTCACGATAATAAGATACCCTTGAGCATATATACATGC ACGACGTTGGTACACTTACGACTCCGTTGGGCTGTCTTGACTAATCCCGAGTTTGTTTCCTTACCTTGTCTGAAGATCAT GCATTTTGAAAATGTTAGCTATCCCAATGAGACCACGTTGCAGAAACTTATCTCAGGCTCTCCAGTTCTAGAAGAATTAA TACTCTTCAGCACTATGTATCCTAAGGGAAACGTTTTACAATTGCGCTCTGATACGCTAAAGAGACTCGATATCAATGAG TTTATTGACGTTGTGATTTATGCACCTCTACTCCAGTGTCTGAGGGCTAAGATGTACTCAACAAAGAACTTTCAGATCAT CAGTTCGGGTTTCCCTGCCAAACTAGATATTGATTTCGTCAATACTGGTGGGAGATACCAAAAAAAAAAAGTGATTGAAG ATATCCTCATCGATATTTCGAGGGTCAGGGATCTAGTTATTAGTAGTAATACTTGGAAGGAATTCTTTCTATACTCAAAG TCAAGACCATTGCTCCAGTTTCGTTACATATCCCATTTGAATGCTAGATTTTATATATCTGATCTTGAAATGTTGCCAAC GCTTCTTGAGAGCTGCCCAAAACTTGAATCTCTCATATTGGAAATGGTCAAGAACCAATCCACGCGACGTCATGGGGAGA AGCGAGAACCAAATGTGATGGTTTCAACAGTGCCTTGGTGTTTGGTATCGTCGCTCAAGTTTGTGGAATTGAAACGTTCA ATCCCAAGGTATGAAGGAGAAATGGAGCTAGTAAGATATGTCCTGACGAATTCAACAGTCCTGAAGAAATTAAGGCTCAA TGTTTACTACACGAAGAAGGCAAAGTGTGCCTTCCTTACAGAACTCGTTGCAATACCAAGATGCTCTAGCACATGTGTAG TCCTTGTTCTTTAATTACTTGAAAGGTATTGATGAACTTTTCGTTTAAGGTCAATCTTTTGGTCGTTAAATTAGTTGATT TTCGGAGGCTTTGAGCCACATGTAAATTTTGCA ..............................................................N................. ...........................i..N...................................i............. ...............N................N............................................... .......................................................N........................ ................................................................................ .........N.....................................N........N.......i....N.......N.. ..............................N.............................................i... ..............................................................................N. ...........N..............i..................................................... ..............i............................................................N.... ...................N...............................i............................ ................................................................................ ................................................................................ ........................................N............................i.......... ...........................................N...........................N........ ............N....N.............................................................. ..........N..........i...............N.........................................N ............................................................N...........N....... ...............................N................................................ ...................N............. Pos Score Pred ------------------------ 63 0.453 - 108 0.511 Yes 111 0.488 - 147 0.502 Yes 176 0.429 - 193 0.212 - 296 0.444 - 410 0.402 - 448 0.330 - 457 0.233 - 465 0.794 Yes 470 0.267 - 478 0.099 - 511 0.138 - 557 0.635 Yes 639 0.324 - 652 0.489 - 667 0.586 Yes 735 0.511 Yes 796 0.201 - 820 0.130 - 852 0.640 Yes 1081 0.468 - 1110 0.571 Yes 1164 0.458 - 1192 0.228 - 1213 0.266 - 1218 0.331 - 1291 0.445 - 1302 0.653 Yes 1318 0.436 - 1360 0.233 - 1421 0.236 - 1433 0.296 - 1472 0.115 - 1540 0.052 -

Explain the output -- Processing locus 384: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACTCAGAGTTTTGATGCGTTCCAATTCTCGATTACATGCGTCTTGCATTCTCTCTGGAATTATCAATAGTGCGTTTCTA TTTATGATTTCTGGATTTTGAATTCTGCGTTGTTGTTGTGATGCTATGTCTCAGAGAATTAGCTTCATGATGGCGGATAC TGAAATGCAAGAGCAAGATGTGCCGTCTGGTACCAAAGGTGTTGTAGAGGAGCCTTCAGAACTAGAGAAAGACCTAAACT CTATTGAAAGACCTAAGTCCCCTGTTCCAGAGGATACGACTCATACTTTGGATAGTGATGTTCATTTATCTGATGCTCCC ATTGCTAATCAAATTGAGGCTAATGAAGAGGTTGGTGGTCAGAACAGTGTTGATGGGAGGAATGGTGATGTTGATCAGTC TGAAAAGAAGATTACTAGTGATGGCGGTCAAGAGGAGACAACACTTGGGGAATCAAATCCTTTGAAGGGGGATCCGTCGA GTCCTCATGTTCCTGAAGAAAGTGTTAAGAAGTGGAAGACATGGTTGTTGAGTGATGCTGAGGCCAGGGAAGTTGATGAA GCAGGGGCACCACAGGATCAAGAGGCGTTTATTAAAGAAGTGGAGGCCTTCAACAAGGAGAATTTCCTGGAATTTAAAGC CCCAAAGTTTTACGGACAGCCCTTAAATTGTCTCAAGCTTTGGAGAGCCGTTATCAAGTTAGGTGGCTACGATGTGGTCA CCACATCTAAGCTATGGCGGCAAGTTGGAGAATCGTTCCATCCTCCAAAAACATGCACTACAGTCTCCTGGACATTCCGC ATTTTCTATGAAAAGGCACTTTTGGAGTATGAGAAGCATTTAAGGCAAAATGGTGAGCTTAACCTTCCTGGTTCAGCTTC TCTTCCGTCTTCAGGCATTGAAAAGGAGGCAAGTAGCCATCAAGCTTCAGGGTCAGGCAGAACAAGAAGAGATGCTGCAG CTCGTGCTATGCAAGGTTGGCATTCTCAGCGCCTTCTTGGATCTGGGGAGGTTACTGAGCCTATTGTTAAGGAGAAAGGC TTGAATTCAACCCCAAAGCAAAAGAACCTCAAAAACATTGGTGTGCAGAAACAAAAAACAACAACCGGCATGGACCTTGT TTTTTCACACGAATCAGAAAAACAGTCAACTGCCGAGGTTATAGATGTTGGACCCCCTGCAGACTGGGTGAAGATCAATG TCAGAGAAACTAAAGACTGCTTTGAGATATTTGCCCTGGTACCTGGACTTCTTCGCGAAGAGGTCCGTGTTCAGTCAGAC CCTGCAGGGCGGCTAGTTATAGCAGGCCAACCTGAGCAACTTGACAATCCTTGGGGAATCACACCCTTCAAAAAGGTTGT GAACTTTCCCGCAAGAATCGATCCGCTGCACACGTCAGCGGTTGTGAGCCTGCATGGTCGACTGTTTGTGCGAGTCCCAT TTGAGCAGTGACCGGTCTCATCCCAAGTGAGCTAGTTCTTTATTTGTCTCTTTGTTTTCTTTTAGGAATTCGTTTGTATC AGAACTAAAAACAAACGATAGCAAATGTGA ..............N.....................N........................................... ...N....................................N....i....................i..i.......... ....i............N.............................................................. .........................................................N..............N....... ......................N.............................N........N.....N............ ....................N........................................................... ......N.................................N.............N....................N.... ................................................................................ .......................................................................N........ .............i......................................N........................... .......N....................N....................i.............................. .......................................................................N........ ........i....................................................................... .....................................................................i.......... ............................................N................................N.. ................................................................................ ................................................................................ .....................................................N.......................... ................................................................................ ........................N..... Pos Score Pred ------------------------ 15 0.215 - 37 0.412 - 84 0.296 - 121 0.233 - 126 0.805 Yes 147 0.744 Yes 150 0.816 Yes 165 0.847 Yes 178 0.310 - 298 0.100 - 313 0.131 - 343 0.121 - 373 0.342 - 382 0.118 - 388 0.111 - 421 0.191 - 487 0.084 - 521 0.432 - 535 0.230 - 556 0.099 - 712 0.391 - 734 0.847 Yes 773 0.295 - 808 0.254 - 829 0.448 - 850 0.648 Yes 952 0.277 - 969 0.711 Yes 1110 0.725 Yes 1165 0.453 - 1198 0.394 - 1414 0.190 - 1545 0.076 -

Explain the output -- Processing locus 385: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 386: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTGATGATTATAGATTAAAATTTTACTATAGTTTATTTTCTGCATATAAAACGTGTCTGAAACCAGCTTATGTATATG AATTTGATACTGATTACTTAATATGTGTTACTTAATATTAACGCAGAGGAAAAGAGAAAGAGAGCAATGGCAGGAGATCA ACTAAACGTGCTAAACGCACTCGACGTCGCCAAAACGCAATGGTACCATTTCACGGCGATTATAATCGCCGGAATGGGAT TCTTCACCGACGCTTACGACCTTTTTTGTATCTCACTCGTCACAAAGCTTCTTGGCCGTATTTACTATCACGTGGACGGC TCAGAGAAGCCAGGAACGCTGCCACCAAACGTCTCAGCCGCTGTTAATGGAGTCGCCTTCTGCGGTACACTCGCAGGCCA GCTCTTCTTTGGCTGGCTCGGTGACAAGCTTGGGAGGAAAAAAGTTTACGGTATGACCCTCATGGTCATGGTTCTTTGTT CCATCGCTTCGGGTCTATCCTTTGGGAGTAACCCAAAAACAGTGATGACTACACTATGTTTCTTCCGGTTCTGGCTTGGT TTCGGCATTGGTGGTGATTATCCGTTATCCGCCACGATTATGTCTGAATACGCTAATAAAAAGACCCGTGGTGCGTTTAT AGCCGCCGTTTTTGCTATGCAAGGGTTTGGAATCTTGACCGGGGGGATTTTTGCCATCATCGTGTCTGCCGCTTTCGAGG CTAAATTTCCAGCTCCAACCTATCAAATAGATGCCTTGGCATCCACGGTTCCTCAGGCGGATTATGTGTGGCGGATCATA CTGATGGTGGGTGCTTTGCCTGCAGCGATGACGTATTACTCAAGGTCCAAGATGCCTGAGACTGCCCGGTACACGGCTCT TGTGGCCAAGGACGCGAAACTAGCGGCTTCAAACATGTCTAAGGTTTTGCAAGTGGAGATCGAAGCAGAGCAGCAGGGAA CTGAAGATAAGTCCAATTCCTTTGGCTTGTTTTCCAAGGAATTCATGAAACGCCATGGCCTTCATTTGCTAGGAACTACA AGCACATGGTTCCTACTCGACATCGCCTTCTACAGTCAGAACCTATTCCAGAAAGATATTTTCAGCGCAATCGGATGGAT CCCTCCGGCTCAGACAATGAACGCAATTCAAGAGGTTTTCAAGATTGCACGTGCACAAACCCTAATCGCCTTGTGTAGCA CGGTACCTGGTTACTGGTTCACAGTGGCCTTTATTGACGTCATTGGTAGATTTGCGATACAGATGATGGGTTTCTTCTTT ATGACCGTCTTTATGTTCGCTCTCGCCATTCCTTACGACCATTGGACTCACAAGGAGAACAGAATCGGATTCGTTGCTAT GTACTCTCTAACATTCTTCTTCGCCAACTTTGGACCTAATGCAACAACTTTCGTTGTGCCCGCTGAGATCTTCCCGGCTA GGTTTAGATCAACCTGCCATGGAATTTCCGCGGCTTCTGGAAAATTAGGAGCAATGGTTGGTGCGTTTGGGTTCTTGTAC TTAGCACAAAGTCCTGACAAGACCAAGACAGAGCATGGATATCCTCCAGGTATTGGAGTCAAGAACTCCCTCATTGTTTT GGGTGTGGTTAATCTTTTGGGGATGGTTTTTACACTGTTGGTTCCTGAATCTAAAGGCAAGTCTCTCGAGGAAATGTCCG GTGAGAACGAACAAAATGATGAAAGCAGCAGCAGCAGCAACAACAACAGTAACAACGCAGTGTCAACTGCATAGAGAATT CTAAAAGATTTTGTTTTTTACTCTTGTATCTTTGTTTTCTATGTATTTCTTCAGGTTTCTTCTCACTAGCAACTTAAGTT CTCTCTATGTTTA .....N.................................................................N.....N.. ......................N...........................................i............. .......................................N.................................N...... ................................................................................ ..............................................N................................. ....................................................N........N.....N............ ............................................i..........N........................ .......................................i........................................ ................N............................................................... ..............................N................................N................ ...i.......................i.......................i............................ ..................................i............................................. ............................................N.........N......................... .....i....................................................................N..... ................i............................................................... ..............................................................i..i.............. N...........N.................................................................i. ......................................N......................................... ..................i..................................i.......................... ..................................N............................................. ......................i..................................................i...... ...............N..N............................................................. ........................................N....................................... ......N...... Pos Score Pred ------------------------ 6 0.151 - 72 0.493 - 78 0.364 - 103 0.349 - 147 0.689 Yes 200 0.340 - 234 0.370 - 367 0.458 - 453 0.124 - 462 0.228 - 468 0.117 - 525 0.623 Yes 536 0.326 - 600 0.639 Yes 657 0.409 - 751 0.193 - 784 0.116 - 804 0.783 Yes 828 0.518 Yes 852 0.876 Yes 915 0.594 Yes 1005 0.199 - 1015 0.243 - 1046 0.600 Yes 1115 0.388 - 1137 0.714 Yes 1263 0.666 Yes 1266 0.639 Yes 1281 0.200 - 1293 0.377 - 1359 0.774 Yes 1399 0.181 - 1459 0.528 Yes 1494 0.836 Yes 1555 0.109 - 1623 0.642 Yes 1674 0.507 Yes 1696 0.147 - 1699 0.160 - 1801 0.044 - 1847 0.044 -

Explain the output -- Processing locus 387: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGGATCTGTTTCAGAGTCAATAAGCTCTCTCATATTTATATATGCGTGTTTGATTTTGATAAGAAGGGAACTTTGTTGT TGGCTTAATCTGATTTTTTGACATATGCTTGAAGTGTTGCAGGCGATTGTTTTGTTTATCTTGTTTTGGGTTTGGTTGTC TTGGATGGGAGGAAGTAGCTCTAAAGAGTCACCTAGAGGAGGTGGAAGTGGTCGTAGGTATGAACGTAGTGTGTCTGGGA GCTCATCTTATTCGTCTGCTTGGGACCAGAGCAGCTATTATCAGACACCAAACCATCCATCTGCGTCTCCTGTTTCATCC TATAATTCTGGGAGACAAACACCGAAGAATCTTGAGAGGAAGTATTCACGGATTGCTGATAACTACCGTTCCATTGATGA GGTTACCGCTGCTCTTTCACATGCCGGTTTGGAGTCCTCGAATCTCATTGTTGGTATAGATGTCACCAAGAGCAACGAGT GGACAGGGGCGAGATCATTTGGTAGGAAGAGTCTGCATTTCATTGGAACTACTCCAAACCCTTATCAACAAGCTATTTCC ATCATTGGAAAGACTTTATCGGTTTTTGATGAGGATAATTTGATCCCCTGTTATGGATTTGGAGATGCTACAACTCATGA TCAGGATGTCTTCAGTTTCAATCCCAATGATACATATTGTAACGGGTTTGAAGAAGTTCTCATGTGTTACAGAGAGATTG TTCCCCAGCTTCGTCTCTCAGGTCCGACATCTTTTGCACCTATCATCGAAAGGGCCATGACAATTGTGGAAGAAAGTGGT GGCCAGTACCATGTTCTTCTCATTATCGCTGATGGACAGGTGACAAGAAGTGTTGATACAGATAACGGTGGATTCAGTCC ACAAGAGCAGCAGACTATTGATGCCATTGTTAGAGCAAGTGAATATCCTTTGTCTATAGTTCTTGTTGGTGTTGGAGATG GTCCCTGGGACACCATGAGACAGTTTGATGACAACATCCCCGCTCGTGCCTTTGACAATTTCCAGTTTGTGAATTTCACG GATATAATGTCAAAGAACATTGATCCAGCAAGAAAAGAGGCAGAATTCGCGCTTTCTGCCTTGATGGAGATCCCGTCTCA GTATAAAGCCACCCTCGAACTAGGCTTACTTGGACAAAGAACCGGACATTGTCCAGACAGGATTGCGCTTCCACCACCAA CTTATGCTACTCAATCTATGCGCAACAGCCCAAGAACTTCCCGATCAACCAGTTTTCAAAACAAACCATATGATAATGGT GTTTCCTCTACACCACCTTCAACTACTCATAACGAGAGCCAGCAACAGTTTTGCCCCGTATGTCTAGTGAGTGCAAAGAA CATGGCTTTCAATTGTGGTCATCAGACTTGCGCTGGATGTGGGGAAGACCTCCATGTGTGTCCCATTTGCCGGAGCTCAA TCTCGGTTCGTATCAAGCTTTATTAAGATAAGTCTCTCAAGAACTGAAGGAACCCGAGGTGGTCTGAGATCTTTCACAGC CAACACCATCCCAACTTAGATCTCATTTACCAGTGTCCTAACATT ..........................................N..................................... ........................N....................................................... ....i......................................................N.................... ................................................................................ ............................................................................N... ....................N......................................N.................... ................................................................................ ............................N.......................N...........N...........N... .....N....................N..................................i.................. ........................................................i....................... ..........N....................N................................................ ....................N........................................................N.. ..............i............N.................................................... ......i........................................................N................ ................................................................................ ...N.............i...................................................i.....i.... ...........................................................N.................... ................................................................................ ............................................. Pos Score Pred ------------------------ 43 0.141 - 105 0.460 - 165 0.557 Yes 220 0.401 - 397 0.172 - 421 0.234 - 460 0.270 - 589 0.219 - 613 0.388 - 625 0.229 - 637 0.131 - 646 0.146 - 667 0.132 - 702 0.612 Yes 777 0.661 Yes 811 0.191 - 832 0.150 - 901 0.335 - 958 0.219 - 975 0.757 Yes 988 0.175 - 1047 0.692 Yes 1104 0.298 - 1204 0.220 - 1218 0.668 Yes 1270 0.551 Yes 1276 0.590 Yes 1340 0.329 - 1362 0.642 Yes 1397 0.379 - 1414 0.394 -

Explain the output -- Processing locus 388: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 389: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCGATTTTGGAATTTGAATGATAATTTGTGGTATATTTGAACATATTTTTTGAGCAATGGACCTGACCATTTTCTCCA GAGAACTCAAACTATTGCTGAAAAATGCAATGGAATGGGGTGAGACGAGCCCATTCTATATGGTGCAAGAGATTAACAAA CAACACTCATTTGCATCATCCTTCTATTCCAGTCTCACATTTTTTCACCATGTCGAGTTTGGAGGAGCCGCTAAGCTTTG ACAAGTTGCCAAGTATGAGCACTATGGATAGGATTCAGAGATTTTCTTCCGGTGCTTGTCGCCCCAGGGATGATGTCGGT ATGGGACACCGTTGGATTGAAGGAAGAGATTGCACTACCTCTAACAGTTGCATTGATGATGATAAAAGCTTTGCTAAAGA GTCATTCCCGTGGAGAAGGCATACTCGGAAATTATCTGAAGGAGAGCATATGTTTAGGAATATTTCGTTTGCTGGAAGGA CTTCTACTGTTTCTGGGACTTTAAGGGAATCCAAAAGTTTTAAGGAGCAGAAGTATAGCACATTTAGCAATGAGAATGGC ACGTCGCATATAAGTAATAAGATCTCTAAAGGTATACCAAAGTTTGTGAAGATTGTAGAAGTTGGTCCAAGAGATGGCCT GCAGAATGAGAAAAATATAGTACCCACATCTGTAAAGGTGGAACTAATTCAGAGATTAGTTTCTTCTGGATTGCCTGTTG TCGAGGCTACAAGTTTTGTATCACCAAAGTGGGTTCCCCAGCTTGCAGATGCAAAGGATGTAATGGATGCAGTAAATACT TTAGATGGGGCTCGATTACCGGTTCTGACACCGAATCTAAAAGGCTTTCAAGCGGCTGTATCTGCAGGTGCAAAGGAAGT TGCAATCTTTGCATCGGCTTCTGAGTCATTCTCCTTGTCAAATATTAACTGTACCATCGAAGAGAGTCTCTTGAGATATC GTGTTGTTGCCACTGCTGCAAAGGAGCATTCGGTTCCAGTCCGTGGGTATGTATCTTGCGTTGTGGGGTGTCCAGTAGAG GGACCTGTTCTACCCTCAAAAGTGGCTTATGTTGTGAAAGAACTCTATGACATGGGCTGCTTTGAGATTTCTCTGGGTGA TACAATTGGAATCGGCACTCCTGGTTCTGTGGTTCCAATGCTTGAAGCCGTGATGGCAGTTGTGCCAGCGGACAAGCTGG CTGTTCACTTCCATGATACCTATGGGCAAGCTCTTGCAAACATATTGGTGTCTCTCCAGATGGGAATAAGCATAGTAGAC TCATCAATTGCCGGATTAGGCGGCTGCCCATATGCAAAAGGAGCTTCAGGAAATGTAGCCACAGAAGATGTGGTTTACAT GTTGAACGGTTTAGGCGTCCACACCAATGTCGACTTGGGAAAGCTTATAGCTGCTGGAGATTTCATCAGCAAGCATCTCG GTCGTCCCAATGGTTCGAAGGCTGCCGTTGCCCTCAACCGTCGTATCACAGCTGATGCCTCCAAGATATGAGTTTTATTA GACCATCTCCAATGTATAGCTCTATATCTTACTCGAAAATAGAGTTACTCTCTACAATTTATCCCTCTATTTTCTACTCT AAAAAGAATA ...................N......................................i..................... ........................N....N....i........................N.................... .................................................i.............................. ..............N........i.............................................N..N....... N......................................................N..N..................... .................................................i.............................. .....................................................................N.....N.... .........................................................................N...... .....N.......................................................................... ................................................N........N....i...N............. ....N........................................................................... ................................................................................ ................................................N............................... ............................N.................N....i............................ .....................................i..............N........................... ............N........N.....................................N.................... ...............................N....................N..............i..........i. ..........................i..................................................... .........N............................................N............N............ ...........N.................................................................... .......... Pos Score Pred ------------------------ 20 0.273 - 59 0.661 Yes 105 0.358 - 110 0.434 - 115 0.585 Yes 140 0.251 - 210 0.705 Yes 255 0.445 - 264 0.754 Yes 310 0.267 - 313 0.445 - 321 0.486 - 376 0.111 - 379 0.288 - 450 0.568 Yes 550 0.310 - 556 0.320 - 634 0.169 - 646 0.329 - 769 0.255 - 778 0.315 - 783 0.783 Yes 787 0.257 - 805 0.144 - 1009 0.150 - 1069 0.190 - 1087 0.339 - 1092 0.773 Yes 1158 0.587 Yes 1173 0.470 - 1213 0.163 - 1222 0.479 - 1260 0.400 - 1312 0.412 - 1333 0.201 - 1348 0.538 Yes 1359 0.526 Yes 1387 0.611 Yes 1450 0.141 - 1495 0.194 - 1508 0.150 - 1532 0.286 -

Explain the output -- Processing locus 390: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGCTTTTTTGATTGATGTTCTGCTAAATGATAAGGTTACAAAATTAGGGTTTGGTAATTGGAACAAATAGGAGGCGAGA GAGTAGCAAGTACGTTTCTAAATTTGTGAATTAAGTTATGATGTGGAGTTGCTTATGAGAAATCATGTTTCCATATGTGT AGCACATTGTAGTAACAACACTGACAAAGTTGATGGAGAGTGTAAAACTGGTTCTTGTTCAGTGGTGGAAAATATGGGAT CAGAAAGAGCTAGTAACAATGTGGATGATAATTATGATGAGAAGTCGGAAAATGGCGAAGACTGTGATAATCATTCGTTA GGTAGTTGGAAGGGAAGTGAAATTGTATTTGGTTCCTTTCCTGAAGATTTTAGTAGTGTATTGCAATCTAGACCAGCTGT TGTTGAAACAGCAAGCCCTCGTATGCGTTGGGCTGATATGGCACAAGAGGATGAGTTCGATGAGGAGGAGGAGGAGGAGG AAGAGGAGAGGGATTCGAGTAGGAAAGGTTTTGATGCGAGTTCGATGAAGACTCCAGAGAAGCCTAAGTTGTCTAGAGAT CAGAGAGAGAATCTCAGGCTGATTAATGTGAAGAGAAAGAAAGACTTTATTTGCTTGGAGAGAGTTAAGGGAAAGATTGT TAACGTGCTTGATGGACTTGAGTTGCACACTGGTGTTTTTAGCGCAGTGGAGCAGAAACGGATTGTTGATCAAGTGTATC AACTTCAAGAGAAGGGACGAAGAGGAGAACTTAAAAAGCGTACATTTACTGCTCCACATAAGTGGATGAGAGGAAAAGGA CGTGAGACTATACAATTTGGCTGTTGCTACAACTACGCTCCAGACAGAGCTGGAAACCCACCGGGTATCCTTCAACGTGA AGAAGTGGATCCACTACCTCATCTTTTCAAAGTAATCATTAGAAAGTTGATTAAATGGCATGTACTGCCTCCAACTTGTG TGCCCGATAGCTGCATTGTGAACATCTATGATGAAGGTGACTGCATACCTCCTCACATCGACAACCACGACTTTCTCCGT CCCTTCTGTACCATCTCTTTCCTCAGTGAATGCGATATCCTCTTTGGCTCAAACCTGAAAGTTGAGGGTCCTGGTGACTT CTCGGGTTCATACTCAATACCGCTTCCTGTGGGATCAGTTCTGGTGTTAAATGGAAATGGAGCTGATGTAGCTAAGCACT GTGTACCTGCAGTTCCTACAAAAAGGATATCAATTACATTTAGGAAAATGGATGAGTCGAAACGCCCGGTTTGGTTCACC CCAGAGCCTGATTTGCAAGGGATTGAGCCATTGCCATTGGACCTGAACCGGTCTGGTTCTACTTCAAGGTTCTCAAGATT AAACAACCATAACGGTACTAACCAAAGAGGACATGGACGTAGAGGAGGAGGCAATGGTTATGACTCAAGAGGTTACTATA ATCCTGAGAGAAGCAGTGAACACAATGACTCTGGAGACTGGCCATCAAGCCAGAGAAGAGGAATGCCACGTCCTAGTAGA AGAAACTACGGTTAAATTCTTCAACTTCTGAAACTGAAATTTATAAGTTCTTTATTTAATTAACCAATTTTCCATATAAA TAATTAATTTGTGCAACATATATACATAAGTCTT ...............N...........N.................................................... .....................................N..N.............N.........N.........N..... ................................N........................................i...... ..................N.....N........N..N..............N............................ ................................................................................ ......................i..............i............N........N.................... .................................N..........N................................... .........................N...................................................... ...........N.................................................................... .................................................................N.............. ................................................................................ ......................................................N....i.................... ...........................N..i................................................. .............................N.................................................. ..................................................N.....i........N.............. ...............................................i...N............................ ................................................................................ ................................i....................i.....i.................... ........................N.....................................N................. ................................................................................ .................................. Pos Score Pred ------------------------ 16 0.202 - 28 0.215 - 118 0.160 - 121 0.322 - 135 0.498 - 145 0.323 - 155 0.192 - 193 0.225 - 234 0.785 Yes 259 0.292 - 265 0.107 - 274 0.245 - 277 0.108 - 292 0.419 - 423 0.773 Yes 438 0.846 Yes 451 0.197 - 460 0.138 - 514 0.154 - 525 0.247 - 586 0.338 - 652 0.202 - 786 0.434 - 935 0.413 - 940 0.646 Yes 988 0.294 - 991 0.612 Yes 1070 0.449 - 1171 0.160 - 1177 0.535 Yes 1186 0.337 - 1248 0.688 Yes 1252 0.352 - 1393 0.699 Yes 1414 0.796 Yes 1420 0.792 Yes 1465 0.370 - 1503 0.337 -

Explain the output -- Processing locus 391: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTTTGGGCCCATAACACTTATCACAAAAGGCCCATCACTTTAAATCTTCTTCATAATTCACTGCTCTCTTCCTCACTA CACAGTTTTCTATTCTTCTAGAATTTGCTTATGCAGACATTTTCAATTTCACTCCTCCGTCACTCTTCTCCGTTCCTCCG TCACACCGCGATTTTTCACGGCGGAGTTGGAGTTAGATTCAAATTCCTTCGCAGCTTTTCTCCTCTCTCAGCAAGACGAG ATAATCCCGATGTCTCCAGAAAACCCCAACCGAGCAAAAATATGCTCAGAGCGAAGCACATCGGTAAAAATTACTCCTCC TCCTTGTCACCAGTTCTCTCGCCGGAGCATAAACCTTCGCTCTTAGAGTCTCAAGCTATTGGTACTGTCGCAACAGCTCA AGCGAACTTTATGCGCGTCATTGTTCAAGATGTAGCTAATTCTGTGACGAGTGATGATGATAATGATTCTTCAAAGACTG GTGTTGAATTGCTTTGTGTGGTGAGAGCTGTGTTGAAGAAAATACGAAGGAGAGTTTTAGTTGGAGATAAGGTTCTTGTT GGATCTATTGATTGGGTTGATAGAAGAGGAATGATTGAGAATGTGTTTCATCGACGTTCGGAGATTTTGGATCCACCTGT TGCGAACGTTGATCATTTGCTTGTTCTTTTCTCTTTGGATCAACCGAAACTTGAGCCGTTTACTCTTACTAGGTTCTTGG TGGAAGCTGAATCTACTCGTATTCCATTAACACTTGCTTTGAATAAAACTGAACTCATTAGTGAAGAGGAATTGGAAACT TGGAAGATAAGGTTGCGTGGATGGAACTATGAACCATTGTTTTGTAGTGTGGGAACTAAAGATGGACTTGATGATATTGC GTTTGTTCTGAGAGATCAGACTTCTGTGATTGTTGGACCTAGTGGTGTTGGAAAGTCGAGTTTAATCAACGTATTGAGGA GTAATCATGGTGGTGGTGTGGTGGAAGATGAGAATTGGTTTGAGCCTATGTTAGGTAATAAGTGGTTTGATGATCAGCGA GTAGGGGAAGTTTCGAGTAGAAGTGGTAGAGGTAAACATACAACACGAAATGTATCGCTACTGCCGGTTTCTGAAGGTGG TTACCTCGCTGATACTCCTGGCTTTAACCAGCCTAGTTTGCTGAAAGTAACGAAGCATTCACTAGCTCACTGTTTTCCTG AGATACGGAACATGATTGAGAGCGAAAAATGTGGATTCAGAGACTGCTTGCATATTGGGGAACCAGGATGTGTTGTGAAA GGTGACTGGGAAAGGTATCCTTACTACTTACAATTGCTTGATGAGATCAGAATCAGGGAAGAATTTCAGCTTAGGACTTT TGGAACCAAAAGGGAAGATGATGTTAGGTACAAGGTGGGAGACATGGGTGTGAAACATGCTGAACCACGGTTAATGCCTA AGAAGCATAGGAGAGAGTCAAGGAAGAAAACGAAACAGACAATGATCAGTGAGCTGGATGAGTTCGAAGATGAAGACAGT GATTTGTACATAGAGAACGACCCAATCGTCCAAGCCATTGAGAATGAGAATAAAAGACAATGAGAGAACTCAAGTTTCCA CTATAACTAAGTCACATGATGTAAAAGATTGTACAGATCTTTTTAAGTTCTCAGCTCTTTTGTTTGTCTGAATTATTGAC TA ................................................................................ ..............................N................................................. ................................................................................ .........N...............................i...................................... ................................................................................ ..........i..................N.......................N..N.....N................. ................................................................................ ..............................N.........N....................................... ................................................................................ ................................................................................ ....................N.......N................................i........N......... ................................................................................ ......N....................N...................N.....................N.......... .................................................N.............................. ................................................................................ ...........N................i......................................N............ ........................................N....................................... .................i..N......................N............N................i...... .........................................N...............N...........N.......... ...........................................N...............N.................... ...............N..N............................................................. .. Pos Score Pred ------------------------ 111 0.488 - 250 0.419 - 282 0.566 Yes 411 0.549 Yes 430 0.369 - 454 0.086 - 457 0.100 - 463 0.193 - 591 0.433 - 601 0.097 - 821 0.147 - 829 0.132 - 862 0.574 Yes 871 0.080 - 967 0.414 - 988 0.415 - 1008 0.228 - 1030 0.207 - 1090 0.261 - 1212 0.467 - 1229 0.804 Yes 1268 0.332 - 1321 0.235 - 1378 0.589 Yes 1381 0.460 - 1404 0.496 - 1417 0.356 - 1434 0.548 Yes 1482 0.347 - 1498 0.304 - 1510 0.275 - 1564 0.297 - 1580 0.450 - 1616 0.048 - 1619 0.055 -

Explain the output -- Processing locus 392: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAATTTTGAATGATATGTCTCAATGATAGGTAACATGAAGATTGAAACTAAACGGTCCATAAATTTCTAAAGTTGAATG GAAAAAAATGTCTATCTCTAACCATGGAAGGACTTGAATATCGACAACAAAGTAGAAAAAAGAAGCGAACAAGTCAAAAA TGACTTCAAAGACATAACATCAATTCACATTTTGATATATAACAAAGCTTATCCTATTATCGCCTTTATTTATCTTTGTA ATGAACTCCTAAAGCACTTGGGAAAACAAGTAGAGAGAATAAAAGAAATGAAAATAGAGCTAGTATTCATTCCCTCTCCG GCAATTAGTCATCTCATGGCGACGGTAGAGATGGCGGAGCAACTAGTTGATAAAAACGACAACCTCTCTATCACCGTAAT CATCATATCTTTTAGTTCTAAAAATACATCCATGATCACCTCTCTTACATCCAACAACCGCCTCCGGTACGAAATAATCT CCGGAGGAGATCAACAACCAACGGAGCTCAAAGCAACTGATTCCCACATCCAAAGTCTAAAGCCACTGGTGAGAGACGCG GTTGCTAAACTCGTAGATTCCACTCTACCAGACGCGCCTCGTCTTGCGGGATTCGTTGTTGACATGTACTGCACGTCGAT GATCGATGTCGCTAACGAATTTGGCGTCCCTAGTTACTTGTTTTACACCTCTAACGCTGGATTTCTTGGACTTTTGCTTC ACATTCAGTTCATGTACGATGCAGAGGATATCTATGACATGAGCGAATTAGAAGACTCTGACGTAGAGTTGGTGGTTCCG AGTTTGACTAGTCCTTATCCGTTGAAATGTCTTCCTTACATTTTCAAATCAAAAGAGTGGCTCACTTTTTTTGTAACTCA AGCGAGAAGATTCAGAGAAACTAAGGGCATTTTGGTAAACACGGTTCCTGACTTGGAACCTCAAGCGTTGACGTTTCTTT CCAATGGTAACATTCCACGTGCTTACCCAGTAGGACCATTGTTGCATCTCAAAAACGTAAATTGTGATTACGTGGACAAG AAGCAATCGGAGATTTTACGGTGGCTAGACGAGCAACCGCCAAGATCTGTAGTGTTCCTCTGTTTCGGGAGCATGGGAGG GTTCAGTGAGGAACAAGTGAGAGAAACCGCATTAGCTCTCGATCGAAGCGGCCACCGGTTTCTTTGGTCTCTCCGTCGTG CATCTCCGAATATATTGAGAGAGCCTCCCGGAGAATTCACAAACCTAGAGGAGATTCTCCCAGAAGGGTTTTTCGATCGG ACGGCTAACAGAGGAAAGGTTATCGGATGGGCTGAACAGGTGGCCATATTGGCGAAGCCGGCGATCGGAGGTTTTGTTTC TCACGGCGGATGGAATTCGACGTTGGAGAGTTTGTGGTTTGGTGTTCCGATGGCGATTTGGCCGCTTTACGCTGAACAGA AGTTTAACGCTTTCGAGATGGTGGAAGAGCTTGGTTTGGCTGTGGAGATCAAGAAGCATTGGCGAGGAGATCTTTTGTTG GGGAGGTCGGAGATTGTGACGGCGGAGGAGATTGAGAAAGGAATCATATGTTTGATGGAGCAAGACAGTGACGTCAGGAA GAGAGTGAATGAGATCAGCGAGAAGTGCCACGTGGCTTTAATGGACGGTGGATCGTCAGAAACTGCTTTGAAAAGATTTA TTCAAGACGTAACGGAGAATATTGCTTGGTCGGAAACTGAAAGCTAGGGAAATCGATTGAAAATGTTGTATAATGGGCCT GAATAATGGGCCATTGTCAAGCTGTTTTATTTCTGTGAACATGTATTTTTAAGTTTTGTGTATGAA ..........N....N.......N...........N.........................................N.. .......i...............N.......................................................N ................................................................................ N..............................................i................................ ...............i..............i................................................. ...............................i................................................ ................................................................................ ...............................................................N..............i. .....N.......................................................................... ...........i......N..............N....N......................................... ..........................i..................................................... ................................................................................ ...N............................................................................ ........................................................................N....... ................................................................................ ................................................................................ ..........................i..................................................... .........N.......................................i.............................. .................i.............................................................. ...............................................N......N......................... ........N...............................N....................................... ..............................................................N.........N....... .....N..................................N....................N.... Pos Score Pred ------------------------ 11 0.355 - 16 0.345 - 24 0.465 - 36 0.411 - 78 0.314 - 88 0.531 Yes 104 0.357 - 160 0.390 - 241 0.144 - 288 0.726 Yes 336 0.561 Yes 351 0.615 Yes 432 0.669 Yes 624 0.257 - 639 0.632 Yes 646 0.219 - 732 0.743 Yes 739 0.463 - 754 0.214 - 759 0.357 - 827 0.582 Yes 964 0.197 - 1113 0.330 - 1307 0.626 Yes 1370 0.423 - 1410 0.505 Yes 1458 0.717 Yes 1568 0.395 - 1575 0.162 - 1609 0.266 - 1641 0.494 - 1743 0.203 - 1753 0.057 - 1766 0.083 - 1801 0.039 - 1822 0.042 -

Explain the output -- Processing locus 393: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGGGGTTTAACGATAAAAGTCATAAAACCCTCTTCTTTTTTCCAGAATGATGTGTCCATCTCTCTGATATCCTCTTATT GGTCTTTAACGTAATATTTGACCAGAAGCAGGGGTAAATATGGAATTAACATCATTCCAAAAATCTCCTAGTTCTAACGA TGTTGTTTCGTTCTCTGTTTCACTGGTGAGAAACTCGATGGCGAGGAGGCGACGTTCATCGGCGGCGGAGAGTTTGAAAC GGCGGAACGACGGATACGAGTCACTGTGTCAAGTCGTACAACAAGATTCAGATCGGAGACTCATCACCATTTTCGTAATC TTCTTCATTGTGATTCCCGCCGTTTCGATTGCTGTGTACAAAGTCAAATTCGCTGATCGAGTCATCCAAACGGAATCATC AATACGTCAAAAGGGAATCGTTAAAACTGATATTAATTTCCAAGAAATCCTCACTGAACATTCCAAGGCTTCAGAGAATT CAACTAGGCACTATGATTATCCAGTGTTAGCTTACATTACTCCATGGAACTCTAAAGGCTATGATATGGCAAAGATATTC AACTCAAAGTTTACACATTTATCGCCGGTATGGTACGACCTGAAGAGCCAAGGGTCTGGATTGGTTTTGGAAGGGAGACA TAATGCTGATAAGGGATGGATTCAAGAGCTTCGGTCAAGAGGCAATGCACTGATATTACCAAGAGTTGTTCTAGAAGCAA TTCCTGGGGAGATGCTTAACAAAAAAAAACTAAGAGAGAAAGCTATCAGTCTTATTGTCACAGAGTGCAAGGAAATGGAA TACAATGGTATCGTGTTAGAGTCTTGGTCAAGGTGGGCAGCTTATGGCGTTTTGCATGACCCTGATTTGCGGAAAATGGC ACTGAAATTCGTAAAACAACTTGGAGACGCTCTTCACTCAACGAGCTCACCAAGAAACAACCAGCAACATATGCAGTTCA TGTATGTCGTTGGTCCGCCTCGTTCAGAGAAGCTCCAAATGTACGATTTTGGGCCAGAAGATCTTCAATTCTTGAAAGAT TCGGTTGATGGGTTCTCTCTTATGACCTATGATTTCTCAAATCCCCAGAACCCTGGCCCCAATGCTCCTGTCAAGTGGAT AGATTTAACTCTCAAGCTCCTGCTTGGGTCATCCAACAACATAGACTCAAACATAGCCAGGAAGGTTCTTCTTGGTATCA ACTTTTACGGCAACGATTTTGTAATCTCTGGAGATTCAGGAGGTGGAGGAGCAATCACCGGAAGGGATTACCTCGCATTA CTCCAGAAACACAAGCCAACTTTCCGTTGGGATAAAGAGAGCGGCGAACATCTTTTCATGTATAGAGATGATAAGAACAT CAAGCATGCTGTTTTCTATCCTACATTGATGTCAATCCTTCTACGGCTTGAGAATGCTCGTCTCTGGGGAATCGGCATTT CAATCTGGGAAATCGGACAAGGTTTAGATTATTTCTTTGATCTATTGTAAGTTCCATACATTGCATCATGTTATGTTTTC TTAATGTTATACTTGATCCTCTTTGTTCACTTAAATCAAATGCTGAGGAACCTTTTGTATCTAGTCTAG ...............................................N..N............................. .......................................N.......................................i .....................................i.......................................... ................................................................................ ................................................................................ ................................................................................ ............N..............................N................N....i.............. .............................N.................................................. ..N............i............................N................................... ...........N..............................................................i..... ....i......................................i...........N...................N.... ......................................................................i........N ...N..................................i......................................... .......N.............N......N................................N.................. ................................................................................ ................................................................................ .........................................................N.........N............ .....N......................N........................N.......................... ...................................................................N....N....... ...N...................................N............................. Pos Score Pred ------------------------ 48 0.250 - 51 0.227 - 120 0.491 - 160 0.693 Yes 198 0.610 Yes 493 0.153 - 524 0.323 - 541 0.210 - 546 0.645 Yes 590 0.288 - 643 0.317 - 656 0.613 Yes 685 0.142 - 732 0.329 - 795 0.661 Yes 805 0.537 Yes 844 0.530 Yes 856 0.276 - 876 0.433 - 951 0.594 Yes 960 0.381 - 964 0.155 - 999 0.556 Yes 1048 0.324 - 1062 0.479 - 1069 0.282 - 1102 0.152 - 1338 0.361 - 1348 0.367 - 1366 0.337 - 1389 0.165 - 1414 0.382 - 1508 0.180 - 1513 0.096 - 1524 0.059 - 1560 0.049 -

Explain the output -- Processing locus 394: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATGATGACCCTTGGGACAATGGCGGATGGGAGTTTAAGGTTGCTGAAGCGAAAGAGCCAAAGCGCGATTTAACTAACAA GGTACAAGGCTCATTTCATAAAAAATATCCTTGCATTGGCAGAGGGTACAAGCTTTACTTGTCATGTGTTTTCAGGAATC TAACGGCTGGGGGTTCGGATTTGGTTTTGAACCCGTTTCTAAACTTGAAACAACCAACTCTTTTCAATCAAGTGTTGAGA AAGAGACGAAAAAGATGGAAAATGGCTCGATTTCCTTTCCAAGCAATGGAGATGTGAATTCTGGAGGAACTTCTTGGGCC TTTAAGCAGCCATCTTTGGAAATTGGAAACGAGAAAGAGGAAAAGGAAGTACAGACAGGCAAGCCCAAGGGAGTCTTGCC ATTGTCCTTTTTCGAGGATGAGAAATCGGAAACCTCTGACACTTTGGTTCATGAAGATAGTTTTGTTTTAGCCAGCGATT TTCCTGTGAGAGAGAAAACTAAGGCTCCAAGTCCAACTGTGTCCATCAGTGACTTAATATCCAGATTATACAGTCAGGTG GAGGAGAAGAATGCTGTTAATATTTTAGAAAACTCTGCAACTGCTTCTAATGAAGTAAATGGTGAGGATGATTCATGGGA ATTTCAGGGCCCCAAAATGCCCATAACAGATTCAGGCATAGCAGAAGGTGCTGATGATTTTGACAGTACTTGGGAGTTTC AAGGGCCTTCTCCAGCCTTGAAAATGTCTGACGTCACAGAAGTTGTCGATGAGTTTGACGATGATTCATGGGAGTTTCAA GGTCCAACACAGCCTGTGAAAGATTCCATGTCGAGGATAGGAGATAATGGTTTGTGGGAATATAAACATAGTTCTGTCGA AAATGAAGTTGGGAATCAATCCTCTGTTCCAAATGGTTTTGGAGAATTGCATGATAAAACAGTAATTCGGATAGAGCCTA ATGATTACCAAGATCTCTTTCATAAGCTGAAGATTGAATTATACCACATTGCTCTATATCATCTCGAGAAATTAAAGGAA GCTCGTGACAAGGCTGCGGATTCTGATGAAGTTCAAAAATGCGACAGTGAAATCGAGGATCTGCAAAACTTGCTGAACAA TGATGTTTTGATCAGTGGAGTCAACCTAGAAAGTCTTCAACCAGGATCTTCTGGCATGACTGAACTTTATAAGGCTCTCC AGGAGCCAAAGTTTCGAGAACTTGATTCAGAAGATCTCCTGACAGAGAGATTGTTATCAGCAGAGAAGGACTGGAAATCA ACAATCGAACTACTCAAACACGCCACACTGACCCTGAAGATCATAAACTTGGGTTCTCTTGAGCAACAGTCGAAATACGC TTCAACTTGGTTTGAAATTTCTTCGACCTGTGCTCAAGAATTGCGACATGCTGCATCCATTTGGAAGCAGGTGATTAAAA ATGATGTCCAAGAAGAAATCCTATCTAAACCTCAAGAGCTATGCTCTCTCAGTGGGAGAAATTTACAGAGTAGTAAAAAT TCTGAGAGCATCAACCAGATTATATAAACCGTGGATTTTATTAGCTCCAACATCATCCAATGTGTTGGCTGTTCTGGATG AGTGTTTGAAGTTGTGGTTGAGCTCTGGGCTAGTAGAAGCTCTGCT ...............................................................N................ ................................................................................ ..............i......i.......................N.....N............................ ................................................................................ .................N................................N............................. ................................................................................ ..........N......................................N........N........N......N..... ................i....................................N.......................... .......................i........................N...........N......N............ ...........................N..................N................................. ..N.............................N.................N............................. N............................................................................... .........................N............i........................................N ..N....................................................i........................ ................................................................................ ................................................................................ ...............................................N................................ N..N....................................N....................................... ...........................................................N.................N.. .............................................. Pos Score Pred ------------------------ 2 0.421 - 5 0.388 - 20 0.428 - 27 0.345 - 144 0.449 - 255 0.900 Yes 262 0.731 Yes 286 0.308 - 292 0.302 - 418 0.205 - 451 0.099 - 571 0.255 - 610 0.374 - 619 0.291 - 628 0.191 - 635 0.347 - 657 0.842 Yes 694 0.131 - 744 0.699 Yes 769 0.281 - 781 0.215 - 788 0.245 - 828 0.466 - 847 0.404 - 883 0.120 - 913 0.172 - 931 0.063 - 961 0.165 - 1066 0.166 - 1079 0.707 Yes 1120 0.293 - 1123 0.278 - 1176 0.764 Yes 1408 0.404 - 1441 0.397 - 1444 0.497 - 1481 0.296 - 1580 0.134 - 1598 0.049 -

Explain the output -- Processing locus 395: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAACAAGAGAACGGACTCATGGACTTGCTTTAAATTTTAATTATTTTAAAATCATTCTATAATGATTAGAGTAAATAAAC TATTAGGACTCTGAATTATAAAATTCGATTTTATATATGCTCCTCCTTGTATCTCTTAATCATAAGTTATCATTAGCTCT GTTCACTAGTGCTATAAAATATATTCTGAGGTTAATAAAACTTTTTTTTCTTTTTATTTTGAAATGTCTCCAGAGATCTG ACATGGAACACCAAGGTTGGAGTTTTGAGGAGAATTATAGTTTGTCCACTAATAGAAGATCTATCAGGCCACAAGATGAA CTAGTGGAGTTATTATGGCGAGATGGACAAGTGGTTCTGCAGAGCCAAACTCATAGAGAACAAACCCAAACCCAGAAACA AGATCATCATGAAGAAGCCCTAAGATCCAGCACCTTTCTTGAAGATCAAGAAACTGTCTCTTGGATCCAATACCCTCCAG ATGAAGACCCATTCGAACCCGACGACTTCTCCTCCCACTTCTTCTCAACCATGGATCCCCTCCAGAGACCAACCTCAGAG ACGGTTAAGCCTAAGTCCAGTCCTGAACCTCCTCAAGTCATGGTTAAGCCTAAGGCCTGTCCTGACCCTCCTCCTCAAGT CATGCCTCCTCCAAAATTTAGGTTAACAAATTCATCATCGGGGATTAGGGAAACAGAAATGGAACAGTACTCGGTAACGA CCGTTGGACCTAGCCATTGCGGAAGCAACCCATCACAGAACGATCTCGATGTCTCAATGAGTCATGATCGAAGCAAAAAC ATAGAAGAAAAGCTTAATCCGAACGCAAGTTCCTCATCAGGTGGCTCCTCTGGTTGCAGCTTTGGCAAAGATATCAAAGA AATGGCTAGTGGAAGATGCATCACAACCGACCGTAAGAGAAAACGTATAAATCACACTGACGAATCTGTATCTCTATCAG ATGCAATCGGTAACAAGTCGAACCAACGATCAGGATCAAACCGAAGGAGTCGAGCAGCTGAAGTTCATAATCTCTCCGAA AGGAGGAGGAGAGATAGGATCAATGAGAGAATGAAGGCTTTGCAAGAACTAATACCTCACTGCAGTAAAACTGATAAAGC TTCGATTTTAGACGAAGCCATAGATTATTTGAAATCACTTCAGTTACAGCTTCAAGTGATGTGGATGGGGAGTGGAATGG CGGCGGCGGCGGCTTCGGCTCCGATGATGTTCCCCGGAGTTCAACCTCAGCAGTTCATACGTCAGATACAGAGCCCGGTA CAGTTACCTCGATTTCCGGTTATGGATCAGTCTGCAATTCAGAACAATCCCGGTTTAGTTTGCCAAAACCCGGTACAAAA CCAGATCATCTCCGACCGGTTTGCTAGATACATCGGTGGGTTCCCACACATGCAGGCCGCGACTCAGATGCAGCCGATGG AGATGTTGAGATTTAGTTCACCGGCGGGACAGCAAAGTCAACAACCGTCGTCTGTGCCGACGAAGACCACCGACGGTTCT CGTTTGGACCACTAGGTTGGTGAGCCACTTTTTTACTTCCTTATTTTTGGTATGTTTCTTTTTTATATCTATCTTTCTGA ACATACTTAAAACGTTCAAGGATGTATTATTATA ..................N..........................................N.................. ....................................N........................................... ...............................................................N................ ..N........................................................................i.... ..............i.......i......................................................... ........N....................................................................... N.................................................i............................. .......................................i........................................ ................................................i.......N......N................ ................................................................................ N............................................................................... ......................N.......N................................................. ..........................................................N.....i...........i... .......................N..N..................................................... .....................i.......................................................... .................................................i.................N........N... ..N............................................................................. ...................................................N............................ .....................N............ Pos Score Pred ------------------------ 19 0.415 - 62 0.297 - 117 0.097 - 224 0.394 - 243 0.416 - 316 0.634 Yes 335 0.557 Yes 343 0.556 Yes 409 0.414 - 481 0.326 - 531 0.843 Yes 600 0.522 Yes 642 0.513 Yes 699 0.765 Yes 769 0.504 Yes 777 0.406 - 784 0.419 - 882 0.883 Yes 896 0.329 - 961 0.464 - 1063 0.129 - 1071 0.482 - 1179 0.387 - 1185 0.632 Yes 1197 0.612 Yes 1224 0.284 - 1227 0.179 - 1302 0.662 Yes 1410 0.791 Yes 1428 0.202 - 1437 0.432 - 1443 0.382 - 1572 0.037 - 1622 0.111 -

Explain the output -- Processing locus 396: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTATTTCCTATTCTCTAATATAATTCATAAAATATATATTATGTGTTGAATTAGTGTTTCTCATAGAGATTTGAGGAC TATATGTTAAGTTTATACTAAGAAAATGGTTTTGGTATGTTTTTGCAGCGTTTCTCTAAAGTAGAATCTTTGGTATTCGG AAAAATGTCGTTTCTGAGTATTGTTCGTGATGTTAGAGATACTGTAGGAAGCTTTTCGAGACGTAGTTTCGACGTGAGAG TATCTAATGGGACGACTCATCAGAGGAGTAAATCTCACGGTGTTGAGGCACATATTGAAGATCTTATTGTAATCAAGAAC ACTCGTTGGGCTAATTTACCGGCTGCGCTATTACGAGATGTGATGAAAAAGTTGGATGAAAGCGAGAGTACTTGGCCTGC ACGTAAACAAGTCGTTGCTTGTGCTGGTGTCTGCAAGACATGGAGACTAATGTGCAAAGATATTGTGAAAAGTCCTGAGT TCTCAGGCAAACTCACATTTCCAGTTTCGTTGAAACAGCCCGGGCCTAGGGATGGAATCATACAATGTTATATCAAAAGA GACAAGTCTAACATGACTTACCACCTTTACCTTTCTCTTAGTCCTGCCATACTTGTTGAAAGTGGGAAGTTTCTTCTCTC GGCAAAGCGCTCACGGAGAGCTACATACACAGAGTATGTAATATCAATGGATGCAGACAACATTTCAAGATCAAGCAGCA CTTACATTGGCAAACTGAAGTCTAACTTTCTAGGGACAAAATTTATAGTATATGATACGGCTCCTGCGTACAACAGTAGC CAGATATTGTCCCCACCAAACCGGAGTCGTAGTTTCAACTCCAAGAAAGTGTCTCCCAAAGTCCCTTCTGGAAGTTACAA CATTGCTCAAGTTACATACGAGCTGAACTTGCTTGGAACCCGTGGACCTCGTAGAATGAACTGCATTATGCACTCTATCC CCTCCTTAGCTCTAGAACCCGGAGGTACTGTCCCTAGCCAACCTGAGTTTCTACAACGTTCCCTTGATGAATCTTTCCGC AGCATCGGTTCCTCAAAGATAGTCAACCACTCGGGAGATTTCACCCGACCGAAAGAGGAAGAAGGAAAGGTGCGACCTTT GGTACTGAAAACTAAACCGCCAAGGTGGCTCCAACCGTTGCGATGTTGGTGCCTTAACTTCAAAGGGAGAGTGACTGTAG CTTCTGTCAAGAACTTCCAGTTGATGTCCGCTGCAACGGTTCAGCCCGGTAGTGGTAGTGATGGTGGAGCATTGGCTACG AGGCCATCGTTATCACCACAGCAGCCAGAGCAATCAAACCATGATAAGATAATACTACACTTTGGGAAAGTGGGTAAGGA TATGTTCACTATGGACTATCGTTATCCTCTCTCTGCCTTTCAAGCGTTTGCCATTTCCCTGAGCACCTTTGATACTAAAT TGGCATGTGAATAAAAGTAGAAAGATCATAAGAGGGACTTAAAACTAATAGGAACCGTTTACGATATGCGAGGGTTTGTT TGTTTTTGTTTATCTGTCTGATCTGGTGTGTTA ..........................................i..................................... ...N.....................N..........N........................................... ....i........................i.................................................. ......N......................................................................... .....................................i....N............N........................ .......................................N.........i.............................. ...................................................N............i............... ............i................................................................... ...................................N..........i...N............................. ...................................................N............................ ................................................................................ .......................................................i...........N............ ..................................................................N............. ................................................................................ ..........................................N..................................... .......................N....................................N................... ........................................N....................................... ....N............................................................N.............. ................................. Pos Score Pred ------------------------ 43 0.613 Yes 84 0.108 - 106 0.323 - 117 0.223 - 165 0.782 Yes 190 0.597 Yes 247 0.378 - 358 0.548 Yes 363 0.342 - 376 0.400 - 440 0.467 - 450 0.523 Yes 532 0.342 - 545 0.543 Yes 573 0.716 Yes 676 0.297 - 687 0.524 Yes 691 0.469 - 772 0.431 - 936 0.598 Yes 948 0.439 - 1027 0.149 - 1163 0.365 - 1224 0.385 - 1261 0.361 - 1321 0.385 - 1362 0.538 Yes 1371 0.725 Yes 1445 0.152 - 1506 0.050 -

Explain the output -- Processing locus 397: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGGTTTTGTTGTTAAAGCTTTTTCTTCTTGGGAGGTGGGTTTTTGCTTCTCTGTGTCGCTAGTTTCAAAAGCTGTCTC CTTTTTCTTGGAATTGAATAAAGCTTCCCCAAAAATCCAATTTTTGTTATGAAGATGCAGAGCCCTAAAATGGAGCAGGA GGAGGTTGAGGAGGAGAGGATGAGGAATAAGTGGCCGTGGATGAAGGCGGCGCAGTTAATGGAGTTTCGGATGCAAGCTT TGGTGTATAGATACATAGAGGCTGGTCTCCGTGTGCCTCATCATCTCGTGGTGCCTATTTGGAACAGTCTTGCTCTCTCT TCTTCCTCCAATTACAACTATCACTCTTCTTCTCTGTTGAGTAACAAGGGAGTAACCCATATCGACACGTTGGAAACTGA ACCAACTAGGTGCAGGAGAACAGATGGGAAGAAATGGCGCTGTAGCAACACGGTCCTTCTATTCGAGAAGTACTGTGAAC GGCACATGCATAGAGGTCGTAAACGTTCAAGAAAGCTTGTGGAATCTTCTTCTGAGGTTGCTTCATCATCAACCAAATAC GACAACACTTATGGTTTGGATAGGTATAACGAGAGTCAGAGTCATCTTCATGGGACAATCTCGGGTTCTAGTAATGCGCA GGTAGTTACCATTGCTTCACTGCCTAGTGCCAGATCCTGTGAAAATGTCATTCGTCCGTCTTTAGTGATCTCTGAATTCA CAAACAAAAGTGTGAGTCACGGCAGAAAGAACATGGAGATGAGTTATGATGACTTTATTAATGAAAAAGAGGCGAGTATG TGTGTTGGAGTTGTTCCTCTTCAAGGTGATGAGAGCAAACCTTCGGTTCAAAAGTTCTTCCCTGAGGTATCTGATAAATG CTTAGAAGCTGCAAAATTCTCAAGCAACAGGAAGAATGATATAATTGCAAGAAGCAGAGAATGGAAGAATATGAATGTTA ATGGTGGTTTGTTTCATGGTATCCACTTTTCTCCAGACACTGTTCTTCAAGAACGTGGTTGTTTTCGTTTACAAGGAGTT GAAACAGACAATGAACCAGGAAGGTGCCGAAGAACAGATGGGAAGAAGTGGAGATGCAGCAAAGATGTTTTGTCTGGTCA GAAGTACTGCGATAAGCACATGCATAGAGGTATGAAGAAGAAGCATCCAGTTGATACTACTAACTCACATGAGAATGCCG GGTTTAGCCCGTTAACCGTGGAAACAGCTGTTAGATCGGTTGTGCCTTGCAAAGATGGAGATGACCAGAAGCATTCTGTT TCAGTCATGGGAATTACACTGCCCCGAGTTTCTGATGAGAAGAGCACTAGCAGTTGCAGTACCGACACTACCATTACTGA CACAGCTTTAAGGGGTGAAGACGACGATGAGGAGTACTTGTCTTTGTTTTCACCAGGTGTTTAGAAACATAGAAATGTTG TCGAGGTTCAAATGATGTTTTTAGTTTCAATCTAAGGATCTTTCTGTGTTAGATGTTAGAAGAAGTCAAATCACAAAAAA ATA ................................................................................ ................................................N.....i..............i.......... ...................N....................N.................N...........N......... ................................................................................ ................................................................................ .......................i.........i.............................................. .....N.......................................................................... ..........N......................................N.......................N...... ............................................i................................... ................................i.....i......N..N...........N................i.. ............................N................................................N.. ...................................N........................N.........i...N..... N..............N................................................................ ..........i..........................N...............N..........N............... ...................N...........i....................................N.....N..... ......................................................N.....N................... ......i...........................N............................................. ..........................N...............................................N..... ...........N..N.....................................N........................... ... Pos Score Pred ------------------------ 129 0.345 - 135 0.536 Yes 150 0.678 Yes 180 0.218 - 201 0.403 - 219 0.153 - 231 0.121 - 424 0.638 Yes 434 0.613 Yes 486 0.280 - 571 0.416 - 610 0.250 - 634 0.185 - 685 0.503 Yes 753 0.879 Yes 759 0.675 Yes 766 0.306 - 769 0.356 - 781 0.193 - 798 0.739 Yes 829 0.166 - 878 0.243 - 916 0.354 - 941 0.279 - 951 0.657 Yes 955 0.401 - 961 0.408 - 976 0.417 - 1051 0.778 Yes 1078 0.243 - 1094 0.341 - 1105 0.487 - 1140 0.459 - 1152 0.609 Yes 1189 0.187 - 1195 0.303 - 1255 0.480 - 1261 0.313 - 1287 0.799 Yes 1315 0.443 - 1387 0.123 - 1435 0.262 - 1452 0.066 - 1455 0.066 - 1493 0.041 -

Explain the output -- Processing locus 398: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCTATTCTAATCTTCGATTCAATTTCGTCACTTTTGAGCACTCTTTTGATTAGAAATCTGAATCATTGCTCCAATCAC TGGTCAATGTAGTTGACTTAGGTCTCTGTAATGGTTAGGTACTGTAAGTCAGGGAATATGTCATCTAGAATCTGCGCTAT GGCCAAACCAAATGATGCTGAGACTCTTTCATCATCAGTAGATATGTCACTTAGCCCGCGAGTTCAGTCCTTAAAACCTT CCAAGACTATGGTTATAACCGATCTTGCAGCCACTCTTGTTCAATCCGGTGTTCCGGTTATTAGACTAGCTGCGGGAGAA CCCGATTTCGACACTCCCAAAGTCGTAGCTGAGGCTGGGATCAACGCGATTCGAGAAGGTTTTACTAGGTATACGTTAAA TGCAGGTATTACAGAACTCAGAGAAGCAATATGTCGAAAGCTAAAAGAGGAGAATGGATTGTCTTATGCGCCTGATCAGA TCTTGGTTAGTAATGGAGCTAAACAAAGTCTCTTACAAGCAGTGCTTGCAGTTTGTTCTCCTGGAGATGAAGTTATAATT CCTGCACCGTATTGGGTGAGTTACACAGAACAGGCGAGATTGGCTGATGCAACGCCCGTGGTTATTCCTACCAAGATTTC TAACAATTTTTTGTTGGATCCAAAGGATCTTGAGTCTAAATTGACTGAAAAATCTAGACTTCTTATTCTCTGCTCTCCTT CCAACCCTACTGGATCTGTTTACCCCAAGAGTTTGCTCGAAGAGATTGCACGGATCATTGCTAAGCATCCAAGACTTCTG GTGCTTTCGGATGAAATATATGAACACATTATTTATGCACCTGCAACACACACAAGCTTTGCTTCTTTGCCTGACATGTA TGAAAGAACTTTGACAGTAAACGGTTTCTCAAAGGCTTTCGCAATGACGGGTTGGAGGCTTGGATATCTTGCTGGTCCTA AACATATTGTGGCAGCTTGCAGTAAATTACAAGGCCAGGTTAGTTCAGGAGCTAGTAGCATTGCTCAGAAAGCAGGTGTT GCTGCGCTTGGGTTAGGCAAAGCTGGAGGAGAAACGGTTGCAGAGATGGTTAAAGCTTATAGAGAAAGACGAGATTTCTT GGTTAAAAGCTTAGGTGATATCAAAGGTGTTAAGATCTCTGAACCTCAGGGAGCTTTTTATCTCTTTATTGACTTCAGTG CTTACTATGGATCAGAAGCTGAAGGTTTTGGTTTGATCAATGATTCGTCGTCTCTTGCACTATACTTTCTTGACAAGTTT CAGGTTGCAATGGTTCCTGGTGATGCTTTTGGAGATGATAGTTGTATCCGAATATCTTATGCCACATCTCTCGACGTTCT TCAAGCAGCTGTTGAGAAGATCAGGAAAGCCCTTGAGCCACTCCGTGCCACTGTCTCCGTTTAACAGTTCGGACACAGAA ATGTGTTTAAGATTGTTTATGATATGTTGTAACTTATTTATCAGCTTTGTCTCCAATCCAATAAAAAGTCTTTTTCTATG CTT ................................................................................ ......N.......................i..........................i....................N. ...........N..N............................i.................................... ........i....................................................................... ...............................................................................N ..............................i......................i...........N.............. ............N.....................................................N............. ..............................................N................................. ................................................................................ ................................................................................ ..........N........N..............N........................................N...N ...........................................i.................................... ................................................................................ .............................................N.................................. ................................................................................ ......N................................N........................................ .........i............N...........N.......................N..................... ................................................................................ N.................N....N.....................................................N.. ... Pos Score Pred ------------------------ 87 0.328 - 111 0.506 Yes 138 0.587 Yes 159 0.445 - 172 0.334 - 175 0.202 - 204 0.665 Yes 249 0.865 Yes 400 0.169 - 431 0.661 Yes 454 0.634 Yes 466 0.211 - 493 0.266 - 547 0.081 - 607 0.142 - 811 0.290 - 820 0.264 - 835 0.189 - 876 0.430 - 880 0.243 - 924 0.521 Yes 1086 0.247 - 1207 0.149 - 1240 0.189 - 1290 0.741 Yes 1303 0.381 - 1315 0.141 - 1339 0.381 - 1441 0.104 - 1459 0.044 - 1464 0.064 - 1518 0.048 -

Explain the output -- Processing locus 399: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTCGATGTAACATTGATTATATTGGGATGATACATATTTAATATACTAATATAAAGAAAGTTTTTTATTTGTGGGGTTA ATATATTTCTGCATCTCATAAATTTACAAAAATAGCCCACAATATATGGGCCGTATTTTCTCACAGGGCCTAGCCCATCA ACGTGTGAGTAAGCGCGTGTTTTTCACGTCTAAAAACCTCCATATCTTCTCCGCTAGCTCCGGAATAGAAGAAATTTTGC AGAGAGAAGCGTCAATGAAGGTCCAACGGTCAACTTTTTTGGCGGTGACCGGAAAATCAAATCTTCATCACTTTCACTCG TCTTCTCAGAGAATTCCCATTACTTCCGAGATCGATTCACGAGAGTTTGTGTCTAAATTACTCGATAGAAAATGGGGATT ACAGTGTCCTGCATCTCCAATTCAGCAGATTAGTGTTTCTTCTGTCAAAGGAATCGATAAATTCTCCTTCTTGAACAACA CTCGTCCACATCTCGGAGATGAAATGTCGAAGTCGAAGCAAGGATCTTCTTTTTACATTCTTAGAGATGATCTTTTACAT CCTCTTGTGAATGGTAACAAAGCTAGAAAGCTTGATGCTTTGTTACCACTTGTTGAAGATCATAAAGTGACTGATTTGGT TACATGTGGAGGTTGTCAAAGTGCACATACAGCTGCTGTTGCTGTTTCTTGTGCTGAGAGAGGTCTAAGATCACATTTGC TACTGCGTGGAGAACAGCCTGATGTTTTGACTGGTTATAACCTTGTTTCAACTATGTATGGAAATGTTCAGTATGTTCCA AGATCGAGGTATGCGAATAGAGAGGAAATGCTAAGAACTTATGCGGATCTTGTTGCTGGGGAAGATGGTACTGTCTTATG GGCTAAAGACATTGTTGAGGGACGTGACACTATGAATGTTGCAAAAATGGATGATTTTTCGAGTATGAAAACTTCTCGTA GAAAGGTTTTGATCGTTAATGAAGGCGCAGGAGATGCTCTTGCATTACTTGGTATGTTTCGGTTAGTGCAGCATTTGTCA CAGGATCATTTACTCGGGAAGAAGAGGCCGGTTAAATTTGTAGTGGATGCTGGTACTGGAACAACTGCTGTGGGTTTAGG AGTTGCAGCTATGTCTTTAGGGCTTCCATGGGAAATCAATGCTGTGATGCTAGCTGATACACTCAAAAATTACAAGAGAC ATGAAGATCACTTGATAGCAGAATTTTCGAGGCAGTTTCCTGGCTCAGTCTTCTGCAGCGGCTTGGATATGAATCAAATG ATCAAATGGATAGATCGTCAACACCCGAGAAAATTTGGGAAGGTCTTGGAAGGAGAAGTGGAGATGTGTCGGAAAATTGC ACAGGAGACAGGAGTTTTAGTGGATCCAATGTATACTTTAGCCGCTTGGGAGACAGCAACAGAGCTTGTGCAGGACGAGA AATCAAGCATTGTAGTGATGCTTCACACTGGAGGCACTCTAGGGATGTTTGGTCTTGCTCAAAGATACAAAACTTGCTTC ACTAACTTGAAAGATTAGGATACAATAACCAAGACTTAGAGCCAGAAATCTAAAGATGGTTGTATAATCATGTTGTATCT TCAATCTTTGTTTTGGGTTCGGTTTTAGCAAACCTAT .....N.....................N.................................................... .............................................N.................................. ................................................................................ ..............i................................................................. .......................................................................i........ ................................................................................ ..................N....i..........................................N............. ..........N.......................N............................................. ...N............................................................................ .....................N...............................i...N.....N........N....... ..........N................i............N.......................N............N.. ...............................i...N..........i...N.............N............... ..................N..............N...................N.......................... ..............................................N................................. ..........N................N..........N.......N................................. i...................................................................N........N.. .....i.........................................................i................ ............................N................................................... .................i..........................N................................... .......................................................N.............N.......... ..................................... Pos Score Pred ------------------------ 6 0.097 - 28 0.307 - 126 0.402 - 255 0.790 Yes 392 0.724 Yes 499 0.190 - 504 0.801 Yes 547 0.116 - 571 0.131 - 595 0.193 - 644 0.245 - 742 0.259 - 774 0.770 Yes 778 0.157 - 784 0.204 - 793 0.177 - 811 0.190 - 828 0.827 Yes 841 0.069 - 865 0.193 - 878 0.286 - 912 0.694 Yes 916 0.130 - 927 0.721 Yes 931 0.117 - 945 0.377 - 979 0.433 - 994 0.230 - 1014 0.410 - 1087 0.202 - 1131 0.305 - 1148 0.425 - 1159 0.328 - 1167 0.145 - 1201 0.508 Yes 1269 0.397 - 1278 0.451 - 1286 0.822 Yes 1344 0.613 Yes 1389 0.334 - 1458 0.500 Yes 1485 0.257 - 1576 0.125 - 1590 0.072 -

Explain the output -- Processing locus 400: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATAACTACAAAACGATCGAGAATTATAATCAGATTTCTCTCACGGATTCCTAATCATTTTTATTATATTTGGATATTT GCTAATTCTTCCCGTGTATAAATCTCATATAAACACGCATCATACATATATATTATGTGCAGCGTCTTTGAGTTTCAAGA CATGGACAACTTCCAAGGAGATCTAACAGACGTCGTACGAGGAATAGGATCAGGCCACGTGTCACCATCTCCTGGACCAC CGGAAGGTCCATCTCCGAGCAGCATGTCTCCGCCGCCAACATCAGATCTCCACGTGGAATTCCCCTCCGCCGCTACTTCT GCCAGCTGTCTCGCAAATCCCTTCGGAGACCCGTTCGTAAGCATGAAGGATCCTCTCATCCACCTCCCGGCCAGCTACAT CTCCGGCGCCGGTGATAATAAAAGCAACAAAAGTTTTGCAATCTTTCCAAAGATTTTTGAGGATGATCATATTAAGAGTC AATGCAGTGTCTTCCCAAGAATTAAGATCTCGCAAAGTAACAATATCCACGATGCCTCCACGTGTAATTCTCCGGCCATA ACCGTCTCCTCTGCCGCCGTAGCAGCTTCGCCGTGGGGCATGATCAACGTTAATACCACTAACAGTCCAAGAAACTGTTT ACTTGTCGATAATAATAACAACACGTCATCATGCTCACAGGTTCAGATCTCTTCTTCCCCTCGGAATCTCGGAATTAAGA GAAGGAAGAGCCAGGCAAAGAAAGTGGTGTGCATACCGGCTCCAGCCGCTATGAACAGCCGGTCCAGTGGAGAAGTTGTT CCGTCTGATCTATGGGCTTGGCGAAAGTACGGTCAAAAACCTATCAAAGGTTCTCCTTATCCAAGGGGTTACTACAGATG TAGCAGCTCAAAAGGTTGTTCAGCTAGGAAACAAGTCGAACGTAGCCGCACTGATCCAAACATGTTAGTCATTACTTACA CCTCTGAGCATAACCACCCATGGCCTACTCAACGCAACGCTCTCGCAGGTTCCACTCGTTCCTCTTCCTCCTCCTCTTTA AACCCTTCTTCCAAATCCTCAACCGCAGCCGCCACTACTTCTCCCTCATCCAGAGTTTTCCAAAACAACAGCAGCAAAGA CGAACCCAATAACTCCAACTTGCCTTCCTCTTCCACTCATCCTCCTTTTGACGCCGCCGCAATTAAGGAGGAGAACGTGG AAGAGCGTCAGGAAAAGATGGAGTTCGATTATAATGACGTTGAAAATACCTATAGACCGGAGTTGTTGCAAGAGTTTCAA CATCAGCCGGAGGATTTCTTTGCCGATCTCGACGAGCTTGAGGGAGATTCTTTGACTATGTTGCTCTCTCACAGTAGCGG CGGAGGCAACATGGAAAACAAAACGACGATTCCAGACGTTTTTAGTGATTTCTTTGACGACGACGAGTCCTCAAGGTCGT TATAAATATTGTTGTTAATGTATACATAGAAATGAAATTATTCATGTAATTCGTTTTGTGTTAAATGACGGTATTTGCCT TTGCAACCTTTGCCTGTGGAAGCC ................................................................................ ......................................................i......................... .......................i........................................................ ..........................................i..................................... ..............................................................N................. .......................................i........................................ ..............................i................................................. ..................................................N............................. ...........N.................................................................i.. .............................................................i.................. ...................N............................................................ ................................................................................ ................................................................................ .................i...............N.............................................. .........................................................i...................... ..........N..................................................................... .................N.............N...........N....................N............... ........................ Pos Score Pred ------------------------ 135 0.783 Yes 162 0.737 Yes 264 0.562 Yes 363 0.844 Yes 463 0.253 - 482 0.550 Yes 532 0.404 - 600 0.589 Yes 671 0.815 Yes 771 0.254 - 812 0.291 - 878 0.563 Yes 942 0.553 Yes 980 0.275 - 1218 0.593 Yes 1234 0.284 - 1338 0.609 Yes 1371 0.456 - 1458 0.107 - 1472 0.094 - 1484 0.050 - 1505 0.071 -

Explain the output -- Processing locus 401: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCATCTGAGCGGTGTCCACAGACTCTATCGTATACCCAAGGGTACTGGATAATTACCTCGTTGCATTTTTTATTTGTTT GGTCAGCATATTTTTAGTTGAATGATTGAATTTGCCGGCCAACGTTTCGACAGTCTCTACACCGTCATGAACACTGAATC AGTTGTCGAGTTCCTTGGGAATGTGACCTTGTTGCAGCGGTTACCTAGTTCCTCTCTGAAGAGAATCTCCGAAGTCGTTG TCTTCAAAGGTTATGACAGAGGTGATTATGTGGTTCGTGAAAATCAAAATGTGGATGGAGTTTATTTTCTCTTGCAAGGA CAGGCTCAGGTTCTGAGATCAGCCGGAGAGGAAAACTATCAAGAGTTCCCTTTGAAACGATATGATTTCTTCGGCCATGG TATTTTCGGGGATGTTTACTCAGCAGATGTTGTTGCTGTGACAGAGCTTACCTGCTTGCTGTTGATGTCTGATCATCGTG CTTTACTTGAAATAAAGTCAGTCTCGGATTCAGATAAGGAACGCTGTCTTGTGGAAGACATACTATATCTAGAACCATTA GATTTGAATGTATACCGGGGGTTCACCCCACCTAATGCTCCAACCTATGGAAAGGTTTATGGAGGGCAATTAGTTGGACA GGCACTTGCCGCAGCATCAAAAACTGTTGAAACTATGAAGATAGTCCATAATTTTCATTGCTATTTCCTTCTTGTTGGAG ATATAAATATTCCCATCATATATGATGTTAACCGCTTACGTGACGGCAACAACTTTGCCACCAGAAGTGTAGATGCTAGA CAGAAAGGAAAAACTATATTCACCTTGTTCGCGTCATTTCAGAAAAAGCAACAAGGTTTTATTCACCAGGAGTCGACCAT GCCTCATACACCAGCTCCTGAAACGCTTCTACCAAGGGAGGAGATGCTTGAACGGCTTGTTACTGAGCCTCTGCTACCTA GGGATTACCGAAACCAAGTTGCAACTGAAATTAGTGTTCCATTCCCTATAGATATTCGATTTTGTGAGCCAAATCGTTCC ACTAAACAGAATAAGTCTCCTCCAAGACTAAAATATTGGTTTAGAGCAAAGGGAAAACTTTCTGATGATGATCAAGCTTT GCACAGATGTGTGGTTGCATTTGCTTCCGATTTGATATTCGCCACTATCAGTTTAAACCCTCACCGGAGAGAGGGCATGA GTGTAGCTGCTCTTAGCCTGGACCACTCGATGTGGTTCCACCGACCTGTAAGAGCAGATGATTGGTTGTTGTTTGTGATA GTGAGTCCAACTGCGACCGAAAGCCGCGGTTTTGCAACTGGCAAAATGTTCAACAGAAAGGGAGAGCTGGTGGTATCATT GACGCAAGAAGCTGTGTTAAGAGAAGCTGTGACTATTAAGCCATCCTTCGGGGCCAAGCTATGAAGCCATAGGATTTTGA TAGTGAGAGAATTGCTGCATCTGTTACTCCTCACGGTCACATTCCAAAGAGTCGTCACTTATACTACATTTGCATATGTT TTT ................................................................................ .....................N............................................N............. ....................N........................................................... ............N..............N....................N.....N......................... .............................................................N..............N... ...........N..............N.....................................i............... ................................................................................ .......N..........................N...........N...........N..................... ..................................N............................................. .....................N..N...............................................N....... ..............................................................................i. ...........................................i.................................... ................................................................................ ................................................................N..N............ ......i.....................................................................i... .............................N...........................N...................... .............................................N.................................. ............................................................N................... ...........................................................................N.... ... Pos Score Pred ------------------------ 102 0.403 - 147 0.393 - 181 0.171 - 253 0.434 - 268 0.437 - 289 0.389 - 295 0.182 - 382 0.104 - 397 0.122 - 412 0.198 - 427 0.115 - 465 0.503 Yes 568 0.353 - 595 0.149 - 607 0.235 - 619 0.151 - 675 0.453 - 742 0.256 - 745 0.327 - 793 0.207 - 879 0.711 Yes 924 0.509 Yes 1105 0.338 - 1108 0.321 - 1127 0.548 Yes 1197 0.703 Yes 1230 0.428 - 1258 0.287 - 1326 0.467 - 1421 0.324 - 1516 0.043 -

Explain the output -- Processing locus 402: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGTCCTTGTAAAAACCCTTTCTCATTTTTGGAAGGTTCTTTCAAAATCCTTTCTTCGATTGCTTTCGGATTCTCGCTCGC TTCTTCGATTTTTGGTTTTGATTCATGGTGAATGTGTTTCCTCCGCTAAGGTTAAACGCTCTTCTTCTTCATGACAGTAC TTTCAGTGACGTCATGACGCTGCATCTGTATCATCACTCCCGTTTTCTATGGAGGAATCTCTGTTTTCCTCGGAGTGTTG GAAACTTGTGTAATCGGAACTGTTCTCTCATCTCTCCTTCCCTTGCACGCTCTGCGAAGTACTATTGCAGTTCCGTAGCT GTTTCTGAATTCTCAAATGAAGCAGCTAGTGGCAGCACATTGACTTCTATAAGTGAAGATGTTACGCCACAGTCGATAAA ATATCCTTTCAAGTCAGAAGAAAGAGTAGCTAGTACTGCTGCTTCTTCAAATGGCAGAGTGATGCTTATTGATGGGACAT CGATTATATACAGGGCTTATTATAAGCTTTTAGCAAGGTTGAATCATGGTCATCTGGCTCATGCTGATGGAAATGCGGAC TGGGTTTTAACAATATTTTCATCTCTTTCTCTTCTGATTGATGTTTTGAAATTTCTCCCATCCCATGTGGCGGTGGTGTT CGACCATGATGGAGTTCCTTATGGCACTACTTCTAATTCATCTACAGGTTATCGTTCTGCAAAAGGCATGAACTTCCGTC ATACTCTCTACCCAGCATATAAAAGTAATCGTCCTCCCACACCTGATACCATAGTACAAGGACTTCAATATCTCAAAGCG TCCATCAAGGCAATGTCTATAAAAGTTATAGAGGTGCCAGGTGTAGAAGCTGATGATGTTATTGGAACACTGGCTATGCG AAGCATTAGTGCCGGTTTCAAAGTCCGAGTTGTCTCTCCAGACAAAGACTTCTTTCAGATTCTTTCTCCCTCGTTGCGTC TTCTTCGACTAACACCACGCGGATCAGAGATGGCTTCTTTTGGAATGGAAGATTTTGCTAAAAAGTTTGGAAATTTGGAA CCAGCACAATTTGTTGATATCATCGCCCTTGCTGGAGACAAGTCTGATAATATTCCAGGAGTTGATGGTATTGGGAATGT GCACGCAGTGGAACTGATATCTAGATTTGGCACGTTGGAGAATCTGTTGCAGTCTGTGGATGAGATCAAAGAAGGAAAAA TAAAGGAGTCTCTAATAGCTAGTGCGGATCAAGCCATTCTTAGCAAGAAATTGGCACTGTTACGGTCTGATCTCCCAGAT TACATAGTACCGTTCGACACGAAAGATCTTACTTTTAAGAAACCAGAGGACAATGGTGAGAAATTGAGTAGCCTGTTAAT CGCGATTGCGGATTACGCAGAAGGATTTTCAGCTGATCCAGTCATCAGAAGAGCTTTCAGGTTGTGGGAAAAACTGGAAG CAGTGCCATGATGATATTTTATGCATCTTTTTTCTTACTCGTTTCTTTGTATTGGGTACATAAAATAGAGTAAGTTCAAG CCAAGCTTAGAAAGTTCTCCCACAAAACAT ................................................................................ ........................i......N......................................N......... .............N..................................N............................... ................................................................................ ................N.........................................N..................... ..................................................N..........N.........N........ .............................................N..............N.....N.....N....... ........................................N.......................N............... .....N..N...........N..............................................i............ ................................................................................ ............i.......................................N..N...................N.... ................................................................................ .............................i..............i................................... ................................................................N...........N... ...........................................................N.................... ................................................................................ ....................................................N........................... ................................................................................ .......N..N.........N........................................................... .............................. Pos Score Pred ------------------------ 105 0.511 Yes 112 0.215 - 151 0.370 - 174 0.489 - 209 0.301 - 337 0.258 - 379 0.302 - 451 0.224 - 462 0.449 - 472 0.339 - 526 0.235 - 541 0.114 - 547 0.135 - 553 0.139 - 601 0.205 - 625 0.354 - 646 0.377 - 649 0.414 - 661 0.417 - 708 0.610 Yes 813 0.746 Yes 853 0.172 - 856 0.233 - 876 0.375 - 990 0.707 Yes 1005 0.857 Yes 1105 0.134 - 1117 0.161 - 1180 0.178 - 1333 0.359 - 1448 0.365 - 1451 0.198 - 1461 0.118 -

Explain the output -- Processing locus 403: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 404: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTTGTTCTGTTTGCCGATGAACATCAATGTTGCTTGGTCATCTAATAGAGGGAAATCATAAAATATTAACCTGGTGGT CCTGGTCAATGGTTTTTTTAAAAGAGTCATTGTTTAAGTCTTTTTTTTTTTTTTTTAATGCTAATAAAAAGGTTTAATCC TCCATGCTGGAGAAGCGATCATCATCAACCGAAAGAGAGTAATAGAGACAAAATGGGTTCATCATCATCTCCATCCCCGA ACGACGCCGTACTGGAATCAGCCCGACCTTTTCTCCGAGGAGAGCTTGAAAAGATCGACCCCAAACTCCCGTCGTTAATC GCCGTCCTCAAAAGCGTCGGTGCCGGCGAGTGTTGGCACAAACACGGAAGCTTCCTAGATCACTTAATCGATATCTACAA GATCCTCAAGCTATGGAAAGCTCCTGAATCCGTCTGTCTCTGTGGTCTATTCCACTCTGCTTACTCCAATTCCTATGTCA ATTTAGCTATTTTCGATCCTTCCACTGGTCGTGACGTCGTCCGTGATCACGTCGGTGAAGCTGCCGAGTCGTTGATCCAT CTCTTCTGCGTTGTACCGAGACAAACTCTGATTCACGATGAGCTTTTGTTTAAGTACTCTGATTGTGAGCTCGTTGAGCA TCTTGATTGTTCTGAGGTTTCGTTGAGGAACGCAAAGGAGAAAGGGATTTTTGATGGAGATGAAGAGTGGAGGAAGAAGA TTAATGCTTTGGTTCCTGAGAACGGTGTTGTTGTGAAGCATATCAAGACAGGTGAAGAAATCGTTGTTTCGAGGAGAGTT GTTGGTGTTTTCTTGTTAATGACAATGGCGGATTTTAGTGATCAGCTTTTTGGGTTCCAAGATGAGTTGTTCTGTAATCA TGATGGGAGACTTGAGTTTAGAGGGAACAACGTGACTGCGTTGTGGCCTGGGAATGGGAAGCCTGGACTGTGGATGAATT CTAATTCGAGAATGGGAGCGATTTATAGTTTGATTGTGAGAGAAGAGGAGATTTTGATGGAAGAGAGGAGAAGGGCTTTT GGTTCTGGGTTTGAAGTGAGGAAGGAAAGAGATGAGGATATAGATCTTGTCGTGCCTCCAGTTTTCAGCTTCTGCACCAA GGTATTGGATGCAAAAGAGCAGATAGAAGCAAGGGAGATGTACTGGGAAGTTGTGAGCAGTGACACAAGCAAAGAAGGGT ACTTGGAGAGAGCAGAGGAGAGATTGTTGGGTTGTATTGAGAAGAACCCATTTGTGGGAGAGCCACATGTGTTGTTGAGT CAAGTGTACTTGGGAAAGAAGAGATTCAAAGAAGCAGAGAGAGAGGCAGAGAAAGGGCTTCTTCTGCTCTTGCAATGGGG AAGTCCTTGGGACAAGAGAATGTCATGGGAAGGTTGGATTGCTTGGGTTAGGGTTCTTCTCATGAAGTCACAGGATCAAT CTTGGCCTGATGTTTCTTGGGGTATCTTGAACTTGGGTCTCGTGCGCTAATAAGATTGTTCTTCACAACTTCCACATCCT TAATAATCATAAAGAATGTTTTAATAAAGAGATTAATAAAGGAAGCCATGTATGTATGTAGTAGATCAA ..................i.........N................................................... ........N................................................N...................... ...i................................................i........................... ................................................................................ ................................................................................ ............N.............................................................N..... ................................................................................ .....................................N.......................................... .....................................................N.....N.................... ...N............................................................................ ..................i.....i....................................N.................N ..N..................................................N...................N...... ...........i............................................i....................... ...............................N................................................ ........N............................i.......................................... ..................................................................N............. ..........................................................................N..... ...................i....N....................................N.................. .........N...................................................................... ...............N...............................N...N...N............. Pos Score Pred ------------------------ 19 0.511 Yes 29 0.167 - 89 0.403 - 138 0.398 - 164 0.559 Yes 213 0.763 Yes 413 0.248 - 475 0.238 - 598 0.207 - 694 0.189 - 700 0.090 - 724 0.164 - 819 0.700 Yes 825 0.691 Yes 862 0.308 - 880 0.451 - 883 0.180 - 934 0.131 - 954 0.347 - 972 0.557 Yes 1017 0.716 Yes 1072 0.232 - 1129 0.181 - 1158 0.630 Yes 1267 0.155 - 1355 0.439 - 1380 0.642 Yes 1385 0.356 - 1422 0.133 - 1450 0.375 - 1536 0.086 - 1568 0.054 - 1572 0.096 - 1576 0.051 -

Explain the output -- Processing locus 405: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTTCAATCATACACCCATGAATTCGCACATATACGTTGATGTATATGAGATTATGAAAAATTCACGTACACATTACAT GTATCTTATAGACATCGCTAAGAGCATAGAGAGAGAGAGAGTTGGGGGAAAATGGGGGCCAAAAGCAAAAGTTCGAGTAC GAGATTTTTTATGTTTTATCTTATACTAATATCATTGTCGTTTTTGGGTTTGCTCTTAAACTTTAAACCTCTGTTTCTGC TCAACCCCATGATCGCTTCTCCTTCGATAGTTGAGATTCGTTATTCTTTGCCGGAACCGGTTAAACGGACTCCGATATGG CTCCGACTCATTAGAAACTATCTTCCGGATGAGAAAAAGATCCGAGTGGGTCTTCTCAACATCGCAGAGAACGAGCGAGA GAGCTACGAGGCAAGCGGGACGTCGATCTTGGAGAATGTCCACGTGTCGCTCGATCCTCTTCCGAACAATCTGACATGGA CGAGTTTATTCCCGGTTTGGATCGACGAGGATCACACGTGGCACATTCCTAGTTGTCCAGAAGTCCCTCTCCCTAAGATG GAAGGTTCCGAAGCTGACGTGGACGTCGTCGTTGTCAAAGTCCCGTGCGATGGTTTCTCGGAGAAGAGAGGGTTAAGAGA CGTTTTCAGGCTACAGGTGAATCTGGCGGCAGCGAATCTTGTGGTGGAGAGTGGTCGGAGGAATGTTGATCGGACTGTGT ACGTTGTCTTCATCGGATCTTGTGGGCCTATGCATGAGATCTTTAGGTGTGATGAGCGCGTGAAGCGCGTGGGGGACTAT TGGGTCTATAGGCCTGATCTTACGAGGTTGAAGCAGAAGCTTCTCATGCCTCCTGGTTCATGTCAGATTGCTCCGCTAGG TCAAGGAGAAGCATGGATACAAGACAAGAACAGAAATCTCACATCCGAAAAAACTACATTATCATCATTTACTGCCCAAC GTGTCGCTTACGTGACGTTACTACACTCATCGGAGGTATACGTATGCGGAGCAATAGCCTTAGCACAAAGCATAAGGCAA TCTGGATCAACCAAGGACATGATTCTCCTCCACGATGACTCTATAACCAACATCTCTCTCATTGGCCTAAGCCTTGCTGG CTGGAAACTACGGCGAGTGGAGAGAATTCGTAGTCCTTTTTCCAAGAAGCGTTCTTACAATGAGTGGAACTACAGTAAGT TACGTGTGTGGCAAGTGACAGATTACGACAAACTAGTGTTTATAGACGCAGACTTCATCATCGTCAAGAATATTGATTAC CTTTTCTCCTATCCTCAACTTTCTGCCGCTGGCAATAACAAAGTCTTGTTCAACTCAGGAGTCATGGTTCTGGAGCCATC AGCTTGTTTATTCGAGGATTTGATGCTTAAATCATTCAAGATCGGGTCATACAACGGGGGAGACCAAGGATTTCTGAACG AATATTTCGTGTGGTGGCATAGGTTATCAAAACGTTTAAATACGATGAAATACTTTGGTGATGAAAGCAGGCATGATAAA GCGCGCAATCTTCCAGAAAATTTAGAGGGCATACACTACTTGGGACTAAAACCATGGCGATGTTACAGAGACTACGATTG TAACTGGGACTTGAAAACGCGACGTGTGTATGCAAGCGAGTCGGTGCATGCGAGATGGTGGAAAGTGTACGACAAGATGC CTAAGAAGCTGAAAGGTTATTGTGGTTTGAATCTTAAGATGGAGAAGAACGTTGAGAAGTGGAGGAAAATGGCTAAGCTC AATGGTTTTCCTGAAAATCATTGGAAAATTAGAATAAAAGATCCTAGGAAGAAGAACCGTCTAAGTCAATGAGAAGAGTC ATCAAACTCGCTTATTCAACAAGAGACTTAATTTTTTTTTTTTCTTTACGTAAAGATTTTGAAAGGGAATTTAATTTCTA ACAGGTAATTG ..................i.....................N.....N.......i.......................N. ...................................................N............................ ..........N..................................................................... ........i...................................................................i... ............................N................................................... ...................................N.......................................i.... .............................................................................i.. .................................................N.............................. ..............................................................N................. .............................N...N.................N............................ .............................................i.............N.................... ............i................................................................... ...........................................i.................................... ..................N...............N............................................. ...........................................................N.................... ................................................................................ ...............................................................i................ ......................N......................................................... ............................................N...............i...........i....... .....................................................i.....N.................... .............................N.................N......i.....................N... ......................................i.............................i........... ................................................................................ ........... Pos Score Pred ------------------------ 19 0.753 Yes 41 0.222 - 47 0.393 - 55 0.559 Yes 79 0.367 - 132 0.263 - 171 0.090 - 249 0.615 Yes 317 0.821 Yes 349 0.461 - 436 0.307 - 476 0.589 Yes 558 0.747 Yes 610 0.142 - 703 0.494 - 750 0.418 - 754 0.326 - 772 0.095 - 846 0.700 Yes 860 0.427 - 893 0.522 Yes 1004 0.591 Yes 1059 0.273 - 1075 0.295 - 1180 0.145 - 1344 0.676 Yes 1383 0.194 - 1485 0.286 - 1501 0.581 Yes 1513 0.575 Yes 1574 0.601 Yes 1580 0.291 - 1630 0.203 - 1648 0.189 - 1655 0.619 Yes 1677 0.370 - 1719 0.659 Yes 1749 0.677 Yes 1762 0.328 - 1829 0.522 Yes

Explain the output -- Processing locus 406: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCGTGGTTGGTTATTAGACACTAGTTTTAGTTGAATTTCGTAAGAGTTAGGTTTTAAGCTATGTCAGTTGTTTTGCGA TTGGTTTCAGAACAAAACTAGAGACAGAGAAAGTTTTTGTCTTTGGCATCTATGTTTGGGGGTGTCTTGTCTATGCCTGT CAATAATCCTCACTTGCGCAAATCTGGAAGTAGACACATCGTCACTAATCTCGGAGATAATGACTTGAAGACTGCAAGTA TGCTTCTGAGTACTTATGCGAAATTGCAAACTCCTATATTCCTTCGAAGTCTCAAGGTTGCTCTGTATATTGGTGGTCTT TATGTTTGTGGAAAGGTTGGATTGGAATCTGTAATGAAAATGGGCGTAGAGACAAGAGAACTGTTCTTTTATGAGACATT TCTGTATTATAACCCTCTCCTTCTCATTACGCTGATGGTCTGGCTCTGGGGTGTTAATTTGTGGGTTTTTTCTCGGTCTG GAGTAGATTATGCAGCAATCTTTTATCTAGGATCAGATCATCTTAGTCACAGAGAGATATGGAAGGTGGTTTTGTATTTC TCAGCTGTGATTATTCTGATAATCCCTTTCAATATCTTCTACATGTCGTCTCGCTACTATTTGCTTTGGACTTTTTGGCG AATACTCTTCCCTGTGCATGCAGTGACTTTTTCAGACTTCTTTCTAGCTGATATCCTGACTTCAATGTCAAAGGTTTTGT CGGATTTAGAACGTTCAGTATGTCGTATGGTCCATCGACAGGTGGCTACTGTTGCATGGTTTGAAGCTGATTCGGTTTGC GGGAGTCATTCTGCTGCAATTCCCTTGGTTCTTGTTCTACCTTATCTTTTCCGGCTGTTCCAATGCATTCGTCAGTACAA AGATAGCAAGGACATTGCAAACATCTACAATGCTGGGAAGTATTTAACAGCGGTGCCTGTCATCTTTCTCTCAGCCCTCA AGTATTATATCGATCCAGATACATGGACTTACTCCATACAGCCGGCATGGATCCTCTCTGGTCTAGCTAACACTTTCTTC TCCTTCTTTTGGGATATATTACGCGATTGGGATCTAAGTGTCTTCACTCGGATTTTCAAATTCACCAGACCAAATCTTTT CTCCCATCTACTATATGGACGCCGTTGGGTATATGTTTGGGTAATCGGAAGCAATCTGGTACTAAGGTGGACATGGACAT ATAAGTTATCTGCTCATCTCCGTAACAACTACATAACGGTCTTCATCATCACTGCCTTGGAGATTTACAGGCGATTCCAA TGGGCGTTTTTCCGTATAGAGAACGTGTGGTATAAAATCAACAATCCCAAGCATACTGCTCATCAATCTAATCCTCTTTC ACTCCAACATGATATCGACAGTGAACATGAGAAATTACTCGCGCATAGTCACAGCCCCGGTGTATAAAAATCTCAATGTT CTTGTTGTTTTGTGCTAAGTTCTGAAAAGTTTTGAATAACAACACATACAAAGTACTCTGTTACTTATACTGTAACTTGT AACTTC ..............................................................N................. ...................................................N....................i....... ...........................................................N...................i ...............N................................................................ ..................................N............................................. .........N................................................i..................... ..........................................N..................................... .................N..............................................N............... ...................i......N............................N........................ ..............................................................N................. .............................N.................................................. ......................N.......................i................................. ................................................................................ ..............N.................N.......................................N....... ...............................................................................i ................................................................................ ........N.................i................................................N.... ................................................................................ ...... Pos Score Pred ------------------------ 63 0.434 - 132 0.380 - 153 0.518 Yes 220 0.131 - 240 0.510 Yes 256 0.111 - 322 0.261 - 354 0.159 - 360 0.444 - 391 0.122 - 435 0.379 - 490 0.092 - 539 0.522 Yes 603 0.246 - 658 0.267 - 705 0.357 - 740 0.694 Yes 747 0.296 - 776 0.245 - 863 0.349 - 910 0.449 - 983 0.282 - 1007 0.546 Yes 1135 0.482 - 1153 0.301 - 1193 0.423 - 1280 0.539 Yes 1369 0.297 - 1387 0.606 Yes 1436 0.060 -

Explain the output -- Processing locus 407: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAGAGAATCTTGTACTGCTCCTTATCTATGTTTTGCTCTCTTTACCAATTGTTTCAATGTTATGAACAAAACCCATATC TCTAAATAGCAACAATAGTGATTTCATTTAATAATGGATTTTGCCTCTGTACCTATTTATATAGTAACCATGGAGCGGCT GATTTCTCATCCTCCTCTCATGATCGTCTCAGACCTTGATCACACCATGGTTGATCATCAAGATCATGAGAACCTCTCTC TTCTCAGGTTCAATTCATTGTGGGAATACGCTTATCGCCGTGACTCTCTTCTTGTCTTCTCTACAGCAAGATCACCTGTT CTTTATAAAGAATTGAGAAAAGAGAAACCTTTGTTGACTCCTGATATCATTATTACATCAATAGGAACTGAGATAGCATT TGGTAACTCCATGGTTCCTGATCATGCTTGGGTTGAATCCTTGAACAGCTGTAAATGGAACCGGGAAATAGTCTTGGAAG AAACTAGCAAGTTCCCTGAGTTAACTCTTCAGCCAAAAACTGAGCAGAGGCTACACAAGGTCAGCTTTTACATTGATGAA GGTAAAGGTGAGGCATTGACCAAGGAACTATCACAATTGCTGGAGAAACGTGGCTTGGATGTCAAGATAATTTACAGTTG GGGAAAGAATGTCGATGTTATACCGCGTGGTGCAGGAAAAGGAGAAGCGCTTGAGTATCTTCTCAAGAAGCTGCAGGCTG AAGGAATATTCCCGGTCAATACTCTTGCTTGTGGTGACTCTGAACATGATGCTGAACTATTTAGCATTCCTGATGTTCAC GGTGTTATGGTGAGCAATTCCCAAGAAGAGCTATTGAAGTGGCGTTCTGAAAATGCGTTGAACAACCTTAAGGTTATACA TTCAACCGAGAGGTGTGCTGATGGGATTATTCAAGCCATCGGACATTTTAATCTTGGTCCCGATCTATCTCCAAGAGATG TTTCGGAATTCTTGGATCGTAAGATGGACAATGTGAATCCTGGTCACGAGGTTGTGAGATTCTACTTGTTCTACGAGAGA TTGAGACGCGGTGAGATCAAGAACTATGAGACATACATAGCCAGCTTCAAAGATTCATGTCTCCATGCTGCTGTCCTTTT TCATCCATCAGGTGCAGAAAAATCTCTGAGAGACACCATTGATGAGCTGAAGAAGTGTTATGGTGACAAAAGAGGGAAGA AGTTTTGGGTTTGGGTAGATCAGGTTCTGGTAACAGACACCATTCCTGGCAAATGGATAGTGAAGTTTGATAAGTGGGAG CAATGCGAGGATGAAAGCCAATGCTGCAAAACGACTGTCGAATTTACTTCAAAGGGAGGAGATTTGGTGTGGGAGAAGGT GAAGCAGATATGGTCAGAGGAATCAAAGGTGAAAGATGATAACAGTTCTTGGATTCTCTGAATATATTTTGGAGACTGTT CTTCTCTTTCTCATGAGTGAATTGGTATATGAACATTCTTCACGATTTTTCTTCCCTTCCTCATATAGGCATTATAAAGT ............................N............................N....N................. .................................i...................................i.......... ...................N..........................i..................N.............. ................................................................................ ................................................................................ ..........N............N..............................i......................... ...........................................................................N.... ..........................................................N..................... ........N.....N................................................................. .............................................N..N.......................N....... ......i.............................................N........................... ....................N........................................................N.. .......................i......i................................................. .........................N..............................i.......N............... .........................................N.................N.................... ....................................................i........................... ..i.......N.........i........................................................... .........N.........................N............................................ ............N...............N................................................... Pos Score Pred ------------------------ 29 0.144 - 58 0.209 - 63 0.140 - 114 0.793 Yes 150 0.810 Yes 180 0.322 - 207 0.618 Yes 226 0.069 - 411 0.291 - 424 0.197 - 455 0.591 Yes 556 0.162 - 619 0.248 - 649 0.131 - 655 0.070 - 766 0.369 - 769 0.206 - 793 0.135 - 807 0.534 Yes 853 0.152 - 901 0.247 - 958 0.223 - 984 0.791 Yes 991 0.514 Yes 1066 0.359 - 1097 0.584 Yes 1105 0.114 - 1162 0.151 - 1180 0.238 - 1253 0.556 Yes 1283 0.609 Yes 1291 0.237 - 1301 0.684 Yes 1370 0.199 - 1396 0.356 - 1453 0.051 - 1469 0.068 -

Explain the output -- Processing locus 408: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 409: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 410: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTGCAAATTGATTCTAAATTTACTCGACTTTTTTTTTGGCTTATCAATGATTTTTAGTTGCCAGAGTCTTTCTATCTT TTTTTTGTATTCTGACGTTAGACTAAGGTGATGCTAGATGTACTCAAGTTCAATTTTAAATTTTCACAGGTAGTCAGTGT TTTTGTTTCGAACTCTTCCAACATGCAGAAGGAAATTGCTGGTGATGCTCCATCAGCACCAACCACTCGTGTTCGGCATC GGAAGCGAAACAGCGATGTTGGTGCAGGGGCAGGCAAACCAAATGGAAACCATTTACTTGTCAATGACAGCAAAAAATAC AAATCGTTTCTCATTCGAGCATACTCCACTTTCTGGATGATAGGTGGTTTTGCTCTTATAGTTTACCTGGGTCATCTCTA CATTACAGCCATGGTGGTTGTTATTCAGATATTCATGGCACGAGAACTTTTTAATTTGCTGAGAAAAACTCATGAAGATA AACAGCTCCCTGGTTTTAGATTACTGAATTGGCACTTCTTTTTTACAGCAATGCTCTTTGTATATGGTCGAATACTTAGT CAACGGCTAGTCAACACTGTGACTCCAGACAAAGTCCTATATCGACTGGTCACAAGCCTCATCAAATACCACATGGCAAT CTGTTACTCCTTGTATATTTCTGGCTTTGTTTGGTTTATTCTCACGCTGAAAAAGAAGATGTACAAATATCAGTTTAGCC AATATGCATGGACACATATGATATTAATTGTGGTGTTTACTCAGTCCTCATTCACTGTCGCCAACATCTTTGAAGGAATC TTCTGGTTTCTTCTTCCTGCATCACTTATCGTCATCAATGACATATTTGCATATATCTGTGGTTTCTTCTTTGGAAGAAC ACCGTTGATCAAGTTATCACCAAAGAAAACATGGGAGGGTTTCATTGGAGCTTCTATTACCACAGTGATTTCTGCATTCC TGCTTGCAAATATAATGGGTCGTTTCCTGTGGCTGACATGTCCCAGAGAGGATCTATCGACAGGCTGGCTCCTCTGTGAC CCTGGTCCACTGTTCAAGCAAGAGACACATGCTTTACCAGGATGGATTTCTGATTGGCTCCCTTGGAAGGAAATTTCGAT TCTACCAGTCCAGTGGCATGCTCTATGTCTTGGATTGTTCGCCTCTATAATAGCTCCTTTTGGTGGCTTCTTTGCCAGTG GTTTCAAAAGAGCATTCAAAATCAAGGACTTTGGTGATAGTATTCCCGGACATGGCGGAATTACAGATAGAATGGATTGT CAGATGGTGATGGCTGTTTTTGCCTATATATATCATCAATCGTTTGTTGTACCTGAAGTCCTCTCTGTGGATAAGCTCTT AGACCAGATAATCACAAGCCTGACATTGGAAGAACAACAAGCGCTACTCGTGAAGCTTGGCCAAATGTTGCAGGAAAAGG TTATTGGATCTTAGATCATCACAACCCGTCATTGTATACCATATTACTCTGCTTCCGTGTTACGTCTGAAAATTATTCAA AAGAAAGAGAAAAGAAAAAAAAAAGTAGATTTA ................................................N............................... ..............................i......N.......................................... ......................i.....................N................................... ...............N..........................N....................N................ ....................................i........................................... ..........N.......................N....................................N........ ..................................................i............N................ ........................................................................N....... ..........................................................i..................... ...N...i.........N.............................................................. .....................................N.......................................... ..............................i................................................. ..............i......................i.......................................... ............................N............N...................................... .................N......N....................................................... ...................................................N...................i........ ...i.....i...................................................................... ................................................................i............... ................................................................................ ................................. Pos Score Pred ------------------------ 49 0.155 - 111 0.838 Yes 118 0.174 - 183 0.687 Yes 205 0.371 - 256 0.217 - 283 0.154 - 304 0.265 - 357 0.532 Yes 411 0.315 - 435 0.353 - 472 0.256 - 531 0.578 Yes 544 0.304 - 633 0.292 - 699 0.645 Yes 724 0.256 - 728 0.519 Yes 738 0.298 - 838 0.292 - 911 0.842 Yes 975 0.543 Yes 998 0.629 Yes 1069 0.293 - 1082 0.257 - 1138 0.262 - 1145 0.203 - 1252 0.153 - 1272 0.769 Yes 1284 0.671 Yes 1290 0.501 Yes 1425 0.542 Yes

Explain the output -- Processing locus 411: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGAGATAAAGCGTGTGATTGTTTCAAATGAATTTGATAGCTAAAGATGAAACCCTAATCTGTTCTAGTATGGAATATAA TCTATCTTTTTTCGGGAATAAATGGTATGCAGATTAGTGGGTTTAGGTCAAGTTCTTGAAATGGATAAGGAGAAATCTCC TGCACCACCACCTAGTGGAGGTCTTCCTCCACCATCGGGTCGTTACTCTGCGTTTTCACCTAATGGAAGTAGCTTTGCAA TGAAAGCTGAATCATCTTTTCCTCCTTTGACTCCAAGTGGAAGCAATAGCTCAGATGCTAACCGATTCAGCCATGATATT AGCCGAATGCCGGATAATCCACCTAAGAACCTAGGCCATCGCCGAGCTCATTCAGAGATTCTTACTCTTCCTGATGACTT AAGCTTTGATAGTGATCTTGGTGTGGTTGGTGCTGCTGATGGACCTTCTTTCTCTGATGATACTGACGAGGACTTACTCT ATATGTATCTTGATATGGAAAAATTCAATTCTTCTGCTACATCGACTTCTCAAATGGGTGAGCCATCAGAACCGACTTGG AGGAATGAATTAGCCTCGACTTCTAACCTTCAGAGTACACCCGGTAGCTCTAGTGAAAGACCGAGAATTAGACACCAACA CAGCCAATCGATGGATGGTTCAACAACTATCAAGCCTGAGATGCTTATGTCAGGGAATGAAGATGTGTCTGGAGTTGACT CTAAGAAAGCCATCTCTGCTGCTAAACTTTCTGAGCTTGCTCTCATTGATCCAAAACGCGCCAAGAGGATATGGGCAAAC AGGCAGTCTGCTGCGAGGTCAAAAGAAAGGAAGATGAGATACATTGCAGAGCTCGAGAGAAAAGTACAGACTTTACAAAC AGAGGCCACATCTCTCTCAGCCCAGTTGACTCTCTTACAGAGAGATACAAATGGCCTGGGTGTTGAAAACAATGAGCTTA AACTGCGAGTACAAACTATGGAGCAACAGGTCCACCTACAGGATGCTTTAAATGATGCACTAAAGGAGGAAGTCCAGCAT CTTAAGGTATTGACGGGGCAAGGTCCATCAAATGGTACATCAATGAACTACGGTTCTTTTGGATCAAACCAGCAATTCTA TCCCAATAATCAGTCGATGCACACTATCTTAGCCGCACAACAGTTACAGCAGCTCCAGATCCAGTCACAGAAACAGCAAC AACAACAACAGCAACACCAGCAACAACAACAGCAGCAGCAGCAGCAATTTCACTTTCAACAGCAGCAACTGTACCAGCTT CAGCAGCAGCAACGGCTTCAACAACAGGAACAACAAAGCGGGGCTTCAGAGCTAAGAAGACCCATGCCTTCTCCTGGTCA GAAAGAGAGTGTGACATCGCCTGATCGTGAAACTCCCTTGACAAAAGACTGAGTCTAGACTGTGCTAATGTCCAATTTAG TAAGTTACTCTTGGAAAATCTTCTTTTTCATCGCAGGCTCATGGATTTGGGATTTACTGCATTATAGAGTT ...........................N..................i......................N.......... .....................i....N.................................i................... ..............................................................N................i ......................................................N.................N....... ......i..................................................................N...... ......................................N.................N....................... ..i...........i......................................i.......................... ....i........................................................................... ..........i...N.........................i.....i.........N.....N................. ......................................................................i......... .................................i.............................................. ..................................................i....................N........ .................i........................N........N..N......................... ...............................i..........N..................................... ................N............................................................... ................................................................................ ...............................................................i................ ...................................................................N............ ........................................N.............................. Pos Score Pred ------------------------ 28 0.405 - 47 0.594 Yes 70 0.331 - 102 0.592 Yes 107 0.336 - 141 0.596 Yes 223 0.230 - 240 0.874 Yes 295 0.174 - 313 0.240 - 327 0.643 Yes 394 0.133 - 439 0.125 - 457 0.071 - 483 0.685 Yes 495 0.691 Yes 534 0.857 Yes 565 0.643 Yes 651 0.878 Yes 655 0.248 - 681 0.714 Yes 687 0.732 Yes 697 0.253 - 703 0.427 - 791 0.667 Yes 834 0.507 Yes 931 0.772 Yes 952 0.224 - 978 0.809 Yes 1003 0.261 - 1012 0.400 - 1015 0.171 - 1072 0.504 Yes 1083 0.279 - 1137 0.328 - 1344 0.550 Yes 1428 0.207 - 1481 0.036 -

Explain the output -- Processing locus 412: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAGGCAATTTGAAGTTTTCTTCACAGATCCGTCTTAGCTCTTTCTAGAGGTTTGTTGTATACCAAAGATCTCTTTTTT TTTTTGCATTTGCTTTGATGAGTTTGGTCATGGAGATTTATGTGTGTTTGCAGAATGGCGGATTTGATACCATTTTATCT GAATATTGTGGCGTTTCTATGTACTGTTGGAGCTATTGCTTTGGCGATATTTCATATCTACAGGCATCTTTTGAATTACA CGGAACCAACTTATCAGAGATATATTGTACGCATTATCTTCATGGTCCCGGTTTATGCCTTCATGTCATTCTTGTCTCTT GTGTTACCCAAAAGCTCGATATATTTTGATTCCATACGAGAAGTATATGAAGCATGGGTTATCTACAATTTTCTATCGCT GTGTTTGGCATGGGTTGGAGGCCCAGGATCAGTAGTGCTTAGTTTGAGTGGTCGTTCTTTAAAGCCATCATGGTCTCTTA TGACATGTTGCTTTCCGCCTTTAACACTGGACGGGCGATTTATTCGACGATGCAAGCAGGGTTGTCTGCAATTTGTAATT CTGAAGCCTATCCTAGTTGCTGTCACACTTGTGCTTTATGCTAAAGGGAAGTACAAGGATGGAAATTTTAACCCTGATCA AGCATATCTCTATCTTACCATCATCTATACCATATCCTACACAGTGGCTTTGTACGCACTTGTGCTGTTTTATATGGCAT GCAGAGATCTACTTCAGCCATTCAATCCAGTCCCAAAGTTTGTGATCATAAAGTCTGTGGTCTTTCTAACCTATTGGCAG GGTGTTCTCGTTTTCCTTGCTGCAAAATCTGGATTCATAAAGAGTGCAGAGGCAGCAGCTCATTTTCAAAATTTTATAAT ATGTGTCGAGATGCTCATTGCTGCAGCATGTCATTTCTATGCTTTTCCATACAAGGAGTATGCTGGTGCCAATGTTGGTG GATCTGGCAGTTTCTCAGGAAGCTTATCACATGCTGTAAAACTAAATGACTTTTACCATGACACTGTTCACCAGTTTGCT CCTACCTATCACGATTATGTGCTCTATAACCATCAAGATGGTGGTGACGAGGGGACAAAGAAGTACCGATCGAGAACCTT TGTGCCAACTGGCCAAGAGATGGATGCAATGAGAAAAAACAAACCTGTGTATGCAAACAAGATAGATGGTGTTTCAGTCT CAAGTAGTCTATCTTCAGAGGCAAGCTCACCTAAAAGCTCCAGCGTAACTTCTGATCCTGCACGCTCTGATGCTGTGAAA TCTTCCTTGCTGGTGGATGCCTCAGATTCTCTTGAAACAATGTATGATATGTCGCTCATTGACATTGATCTATCTAGTTT CCCCAGCAATGTCCCATCTGCAAGTGAAAGTGGGCCTAGATAGGAGCAGAGAAAATGTAGAAATCACCATTAACAGGTAC AAAACATGCTCCTAATAGTTGTATTCAGATGAATCTTCTGAGGTGTGTTCTTAGTTGGAAGA ................................................................................ .................N...........N.........N..............i......................... ..................N............................................................. .........................................N............N.......N................. ..............................................N......i.......................... .........N...........................................................N.........N ....i............................................N.............................. .....................................N....................N..................... .........................................................................N....N. ................................................................................ ................................................................................ N.........i................N..........N....................N...........N........ ..............................i..............N...........i...................... ................N....................N.......................................... ...................i...N....i.....................N..............i.............. .....................................................................N.......... ................N......................i...N....i............................... ........i.............................................N......................... .....N......................N................................. Pos Score Pred ------------------------ 98 0.237 - 110 0.209 - 120 0.429 - 135 0.533 Yes 179 0.171 - 282 0.357 - 295 0.175 - 303 0.111 - 367 0.166 - 374 0.641 Yes 410 0.274 - 470 0.478 - 480 0.222 - 485 0.672 Yes 530 0.472 - 598 0.283 - 619 0.329 - 714 0.436 - 719 0.439 - 881 0.319 - 891 0.548 Yes 908 0.177 - 919 0.238 - 940 0.327 - 952 0.122 - 991 0.547 Yes 1006 0.434 - 1018 0.556 Yes 1057 0.352 - 1078 0.326 - 1140 0.784 Yes 1144 0.437 - 1149 0.519 Yes 1171 0.286 - 1186 0.591 Yes 1270 0.127 - 1297 0.310 - 1320 0.534 Yes 1324 0.275 - 1329 0.503 Yes 1369 0.540 Yes 1415 0.344 - 1446 0.065 - 1469 0.046 -

Explain the output -- Processing locus 413: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 414: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 415: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGATAAATCCGAACCTAATGATCAATGAACACGACGAGTCATTTATACAAAGGCAACAACAAAATGTGGTTTCTTCTCAG TTTGCTTCCTTGAAAATTTAGCTGTTATATACTAAAAGGCCTTTTGTAAGGCTAAAAGGCCAAGCCTTTGGGCCCATTAA CATCACCGTTTATTCGAAACGTATGTTGTTAAGACTTGAGAGAAGTTTGACGGTGAAAAAAGGAAAATATTTACATATAT ATAGTAAAATTAAAATAGTAGAAAGAAAAAAATCAGAAAGCTACGAAAGAAAAAAAAAACAATCTAGCAACAGAAGAAGC TTCGAGAAGACGATGGCGACGGAGACGAACGAGAATCTGATAAACTCCTTCATCGAGATCACTTCTTCATCAAGAGAAGA AGCTAATTTCTTCCTCGAAAGTCACACTTGGAATCTAGACGCTGCCGTTTCAACTTTCCTCGATAACGATGCCGCCGCAG CCGCCGAACCAAACCCTACCGGGCCACCACCACCTTCTTCTACTATTGCCGGTGCACAATCTCCTTCTCAATCTCACTCT CCCGATTACACTCCGTCTGAAACCTCTCCCTCTCCTTCCCGATCACGATCTGCTTCTCCGTCTTCTCGCGCTGCTCCCTA CGGTCTCCGATCGAGAGGCGGTGCTGGAGAGAATAAAGAAACTGAGAATCCGTCGGGAATTCGGAGTTCTAGGAGTCGTC AACACGCTGGGAATATCCGTACATTCGCCGATCTGAACCGTTCTCCGGCTGATGGCGAGGGCAGTGATTCGGATGAAGCT AATGAGTATTATACCGGTGGACAAAAGAGTGGGATGATGGTACAAGATCCTAAGAAAGTGAAAGATGTTGATGAACTTTT TGACCAAGCTAGGCAGTCAGCTGTAGACAGACCTGTTGAACCATCAAGATCAGCTTCTACAAGCTTCACTGGAGCTGCGC GTTTGTTGTCTGGTGAAGCTGTTTCGTCTTCTCCTCAGCAACAACAGCAAGAGCAGCCTCAAAGGATTATGCACACCATC ACTTTCTGGTTGAATGGTTTCACTGTTAATGATGGTCCGTTAAGAGGGTTCAGTGACCCTGAAAACGCAGCCTTTATGAA TAGCATCTCAAGGTCAGAGTGCCCCAGCGAACTCGAACCAGCAGATAAGAAGATCCCTGTTCATGTCGATCTGGTCAGGC GTGGAGAAAATTTCACCGAACCACCAAAGCCTAAAAATCCATTCCAAGGTGTAGGAAGAACCCTAGGAGCCAGCGGATCT GGTTCAAGTTCAGCACCACAAGCTTCATCTGCCCCAATGAACGTAGCACCAGCACCATCAAGAGGGCTAGTTGTAGATCC AGCAGCACCGACAACCTCTATCCAGCTCAGATTAGCAGACGGAACACGCCTTGTCTCCAGGTTCAACAACCACCACACTG TCAGAGATGTACGTGGGTTTATCGACGCTTCTAGACCCGGTGGCTCCAAAGAGTACCAGTTGCTAACCATGGGGTTCCCT CCTAAACAATTGACAGAATTGGACCAAACCATCGAGCAAGCTGGTATAGCTAATGCCGTCGTCATCCAGAAATTCTAGAC TTACATAACTCATCCCTTCGATTTATGTCTCTCTTTTTTTTTTTTTGCTTTTGTGTTTTTGAACCTGATCGTTGACTTTA GTCTCTAACTATCTGGT .................N......N......................................i................ ................................................................................ ......................N......................................................... ................................................................................ ............i................................................................... ....................................................................i........... ................................................................................ ................................................................................ ................................................................................ ...................................................N....................N....... ................................................................................ ....................................................................N........... .............i..............i..i...........................................i.... ..............................................................N................. ................................................................................ ....................................i........................................... ................................................................................ ......N.............................................................i........... ....................................................N........................... ........................N....................................................... ................. Pos Score Pred ------------------------ 18 0.290 - 25 0.299 - 64 0.599 Yes 183 0.216 - 333 0.531 Yes 469 0.622 Yes 772 0.197 - 793 0.084 - 802 0.140 - 834 0.690 Yes 837 0.625 Yes 865 0.301 - 871 0.193 - 1029 0.445 - 1054 0.646 Yes 1069 0.558 Yes 1072 0.663 Yes 1116 0.683 Yes 1183 0.444 - 1317 0.562 Yes 1447 0.405 - 1509 0.520 Yes 1573 0.214 - 1625 0.077 -

Explain the output -- Processing locus 416: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATTTAATTTGATACTAAAATGTTCTGTATAAATAATAAAGAAAATAATAAATCCTTTCACAAAATCCATTTTTATCTC AAATTAAAATTGAATATTTAATTGAAAACTGTAAAGATGACGTCAACTTCTCAGTCATCATCTCAAAAACGGAAGCTTAA GAAAAATCTATACCTACCACTTCGAACTAACTCTCCTCTCTCGCTTACTGGAATTTGGGGATTAGCTTGGGAAACTTCGC CGGAGATTCATACGTCGTCGTCGTCTTTAGTATCTGTATCGTCAATGGCGTTGCTGAAGTCTTTCATCGATGTTGGCTCA GACTCGCACTTCCCTATCCAGAATCTCCCTTATGGTGTCTTCAAACCGGAATCGAACTCAACTCCTCGTCCTGCCGTCGC TATCGGCGATTTGGTTCTGGACCTCTCCGCTATCTCTGAAGCTGGGCTTTTCGATGGTCTGATCCTTAAGGACGCAGATT GCTTTCTTCAGCCTAATTTGAATAAGTTCTTGGCCATGGGACGGCCTGCGTGGAAGGAAGCGCGTTCTACGCTGCAAAGA ATCTTGTCATCTAATGAACCTATCTTGCGAGACAATGATGTTTTGAGGAGAAAATCATTCCATCAGATGAGTAAAGTGGA AATGATTGTTCCTATGGTGATTGGGGACTATACAGACTTCTTTGCATCTATGCATCACGCGAAGAACTGCGGACTTATGT TCCGTGGGCCTGAGAATGCGATAAACCCAAATTGGTTTCGTCTTCCCATTGCATATCATGGACGGGCATCATCTATTGTC ATCTCTGGGACTGACATTATTCGACCAAGAGGTCAGGGCCATCCACAAGGAAACTCTGAACCATATTTTGGACCTTCGAA GAAACTTGATTTTGAGCTTGAGATGGCTGCTGTGGTTGGTCCAGGAAATGAATTGGGAAAGCCTATTGACGTGAATAATG CAGCCGATCATATATTTGGTCTATTACTGATGAATGACTGGAGTGCTAGGGATATTCAGGCGTGGGAGTATGTACCTCTT GGTCCTTTCCTGGGGAAGAGTTTTGGGACTACTATATCCCCTTGGATTGTTACCTTGGATGCGCTTGAGCCTTTTGGTTG TCAAGCTCCCAAGCAGGATCCACCTCCATTGCCATATTTGGCTGAGAAAGAGTCTGTAAATTACGATATCTCCTTGGAGG TTCAACTTAAACCTTCTGGCAGAGATGATTCTTGTGTAATAACAAAGAGCAACTTCCAAAACTTATATTGGACCATAACG CAGCAGCTAGCACACCATACCGTTAACGGTTGCAATTTGAGGCCTGGTGATCTCCTTGGCACAGGAACCATAAGCGGACC GGAGCCAGATTCATATGGGTGCCTACTTGAGTTGACATGGAATGGACAGAAACCTCTATCACTCAATGGAACAACTCAGA CGTTTCTCGAAGACGGAGACCAAGTCACCTTCTCAGGTGTATGCAAGGGAGATGGTTACAATGTTGGGTTTGGAACATGC ACAGGGAAAATTGTTCCTTCACCGCCTTGATCAGCTGTCGCCGGTAACGTACCTTTTATAACATTAATAAAAAAAATCCC GTCACCTGAATTGTTTACAGAAAAAATGGAATTGATGGGAGTATCACTT ....................i........................................................... ....................................N........................................... ................................................................................ ............................................i........................N.......... ...............................N................................................ .....................................................N.......................... ...................................i............................................ .............N....................N..N............................i............. ...............N.........................................i...................... ................................................................................ ......................i........................N.............................N.. .............................N...N...................................N.......... ..........................................................N..................... ................................................................................ ........................N....................................................... ................................................................................ ..............i.....................N....N.......................i.............. ........................................N..........N........N...............N... ................................................................................ .........................N........N.............. Pos Score Pred ------------------------ 21 0.516 Yes 117 0.406 - 285 0.597 Yes 310 0.345 - 352 0.283 - 454 0.213 - 516 0.522 Yes 574 0.093 - 595 0.207 - 598 0.203 - 627 0.752 Yes 642 0.750 Yes 654 0.332 - 690 0.298 - 717 0.550 Yes 736 0.405 - 778 0.553 Yes 903 0.689 Yes 928 0.227 - 958 0.280 - 990 0.375 - 994 0.273 - 1030 0.113 - 1099 0.161 - 1225 0.145 - 1375 0.566 Yes 1397 0.365 - 1402 0.345 - 1426 0.525 Yes 1481 0.151 - 1492 0.312 - 1501 0.406 - 1517 0.402 - 1626 0.066 - 1635 0.064 -

Explain the output -- Processing locus 417: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAAGATATATTTGGTTACTTTATTCAACTCAATTGTCAAGGAACCTGGATCTAGTGTAAATTCTCTTACATGAGACATC ACTTCATTAGTTCTATAATAATATAATAAACCGATGAAAAGATGGGGAAGTAAGGCAAAGAATTGCCTTGTCGGAGAGGC CAGTGTCCTTAGAGTAATGCTAAACCCTAACATATTGTCTTGGGGCTTGAAGCTCTCTAAAGCAACTTCACCGCTTAAAC ATTGCATCCTTCTCTCCCTCTACATTTTATTTGTTATAAACCCTGGTCCATGGACTTCCCAGCAAATCCATATTTCAGAA ATCTTAAACGTTTATACGATAATGGGAAGATCACCAATGGTTCCTATGGCTGTTCTTGTTTATGTTTCTCTACTGTTCTC TGTTTCTTATTCCTCCACGTTCGTCATCACAAATAACTGTCCCTTTACTATATGGCCTGGAACTCTTGCAGGCTCTGGCA CCCAGCCACTACCCACCACAGGGTTCAGGCTTGATGTGGGACAGTCTGTTAGAATTCCCACGGCTCTAGGCTGGTCAGGT CGTATTTGGGCTAGGACTGGCTGCAACTTTGATGCTAACGGCGCGGGAAAATGTATGACTGGAGACTGCGGCGGAAAGCT GGAGTGTGCTGGTAATGGCGCTGCTCCGCCGACATCTCTTTTCGAGATCACACTCGGTCACGGTTCTGATGACAAAGATT TCTATGATGTCAGTCTCGTTGATGGGTATAACCTTCCCATAGTTGCTCTCCCGACGGGTGGTGGACTGGTTGGCGCTTGT AATGCTACAGGATGTGTTGCTGATATCAATATTAGCTGTCCAAAGGAGCTTCAAGTGATGGGAGAGGAAGAGGCTGAAAG AGGAGGGGTTGTTGCATGCAAGAGCGCTTGTGAGGCCTTTGGGTTGGACCAGTACTGTTGCAGCGGTCAGTTTGCTAACC CGACCACATGCCGACCGTCTTTATACTCTTCCATCTTCAAGAGAGCTTGTCCCAGAGCTTATAGCTATGCCTTCGATGAT GGAACCAGTACTTTCACCTGCAAGGCTTCTGAATATGCAATTATCTTCTGCCCTGGCAGGGTAAAAAGACCAGACAGTCT GAACTCGGATCCTCCCAGCCCACCTCAGAATCAGTTAGGGCAACCTATGGCTCCCCCAACTCAAAATCAGTATGGTCAAC CTATGGCTCCTGCACCTCAGTATCAGTACGGTCAACCTATGGCTCCACCGACTCAAAATCAGTATGGTCAACCTCTGGCT CCTCCGACTCAAAATCAATACGATCAACCTCTGGCTCCTCCGACTCAAAATCAATATGGTCAACCTATGGCTCCACCAAC TCCGCCCCCACCCGGACCAAACGAGGATTCAATGACTCCCCCACCTCAGAATCAGAATGGGGATGGGCAGTTCATGCCTC CTCCCACTTTGGACCAAGATCCAAACGACCAGTATATGAATCCCCCAATTCAGGATCAGAGTCAAAGAGAGACTCAATCT TCATCCGATCTTCTTCGACCTTACCCTGTCTTGTTGCTTCTTGGCTTTAGTCTAACTGCTTTGAGGCAATCTGGCACGAC ATGAAACATAATCATTCTACTTTTTAATTAGAAATGCTACATAACTGTGTAGAGGAATCAAACAGACCATGTTTAATGTC TTCTCAGAGATTTTTTATACTCC ......................................................................N......... .................................N.......i...................................... ................N............................................................... .................................................N.............................. .....................i..............i........i...............N.................. ...................................................i............................ .................................N.............................................. ...............................i..................N...N......................... ..............N.....................................................N........... ...N..N..............N.......................................................... ...............N................................................................ .......N..........................................................i........N..N. ..................................N............................................. ..............................................i........................N........ ..i...................................i........................N................ .......................................................N..........i............. ...............................i........................N.....N..........N...... ...................................N............................................ ................................................................................ N................................N..................................N......N.... ....................... Pos Score Pred ------------------------ 71 0.420 - 114 0.401 - 122 0.711 Yes 177 0.164 - 290 0.317 - 342 0.742 Yes 357 0.664 Yes 366 0.685 Yes 382 0.452 - 452 0.830 Yes 514 0.394 - 592 0.598 Yes 611 0.358 - 615 0.496 - 655 0.241 - 709 0.340 - 724 0.217 - 727 0.224 - 742 0.181 - 802 0.234 - 812 0.395 - 858 0.807 Yes 896 0.216 - 968 0.267 - 1027 0.649 Yes 1036 0.432 - 1039 0.448 - 1075 0.358 - 1167 0.579 Yes 1192 0.286 - 1203 0.643 Yes 1239 0.530 Yes 1264 0.304 - 1336 0.308 - 1347 0.687 Yes 1392 0.744 Yes 1417 0.305 - 1423 0.450 - 1434 0.282 - 1476 0.279 - 1601 0.262 - 1634 0.111 - 1669 0.050 - 1676 0.102 -

Explain the output -- Processing locus 418: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATAAAATGCGTTTATGACCACAATGTGAAGAATGCCACTATTGCAAATTTAAATAATTGATTACGTCGTCCTTATTAAT TGGGCCATAAAAAGGCCTTAAATAAATGTGTCTAATTATAATTCACAAATATCGAAGATGATAAACAAACAGTGTAGTTA TCAACAAGCTTCAAAAATGGGGGAAGAAGAAGAGAACCCTAATTCGATCGACATTCTTCCCGAGCTACTTGAAGAAGTTC TCCTTAGATTGCCCACGAAATCGATCCTCAAATGCAGAATCGTCTCAAAACAATGGAGGTCACTCCTGGAATCGAGTAGG TTCGCGGAGAGGCATATGAGTCTTCAAAACAGCCGCCGGAGAATCTTAGCTGCTTACAACTGCGACTGCGGCGGACGGAG GAAGCTCCTACCCGAGTCACGGTTTGAAGGGGACGAAGAGATTGTCTATCTGCACTGCGACGCCTCACGACCCTCGATGA CTTGCCAAGGTGTGATCTGCTTCCCCGAGCAAGATTGGATCATCGTTTTGAACCCATCGACTAGCCAACTTCGGCGATTC CCTTCCGGCTTGAACCATAACTGCAGATTTAGAATTGGATTATGGAAGACTTTCTCTCCGGGAAACTGGGTAATGGGGTT TGGTAGAGACAAAGTGAATGGGAGGTATAAAGTGGTGAGGATGTCTTTTGCTTTCTGGAGAGTTAGGCAAGAGGAGCCTG TGGTGGAATGTGGTGTTCTTGATGTTGATACTGGTGAATGGCGGAAGCTGAGTCCACCTCCTTATGTGGTCAATGTGGGA AGCAAATCGGTATGCGTGAATGGATCTATCTACTGGTTACACATTCAGACGGTTTACAGAATACTAGCCTTGGATCTTCA CAAAGAAGAGTTTCATAAAGTCCCAGTGCCGCCTACGCAGATCACTGTGGACACTCAGATGGTGAACCTTGAGGACCGTC TCGTACTTGCTATAACCAGAGTTAGCCCTGAATGGATACTAGAGGTATGGGGCATGGATACATACAAAGAAAAATGGAGC AAGACTTACTCCATAAGTTTGGATCATAGAGTTGTTTCCTGGCGAAGGCAGAAAAGGTGGTTCACGCCCGTGGCAGTTTC TAAGCAAGCGAATCTTGTCTTCTATGACAATAAGAAGAGGCTATTCAAATATTATCCAGTGAAAGATGAGATTCGTTGTC TCTCCTTAGACATTTGTGTTCTGTCTCCTTACGTGGAAAACTTGGTCCCTCTTCCGTTAAAGCCAAGCCATCCGCATCCT ACTCCGAAAAATTCAGATTTTGAAATGAGGATATCAAGATGCCGCTTGTTTTCGACGCCAGGTTCTTGGATATCCAAAAT TTTGAAATGGAATGTTATGACTCTAGAGATTTTGTTTACCTCTCTAGCAATAGTTGGTTATATATGCTTACCTCTCTAGG AGTTATTTATCTTGTTTCAAAATATTAGTTGGTTATATATGCTTTCGAGATCTTTCTGATATTTATTCTGCCAACTCATT TTTCTTCCTCTTTCCTTT ......N.......i........N........N............................................... .........................N...............................i...................... ................i............................................................... ...............................N....................i........................... ...............i................................................................ ............................................................................i... ................................................................................ .........................................i..............................i....... .................N......................i....................................... .......N.............N...............i.........................N........N....... ...........i.......N............................................................ ..........................................................i..................... ...............................i..............i......N...................i...... ................................................................................ .......................N.........................................N.............. ................................................................................ ........................N.............N......................................... ......i....N....N..............................................N................ ......................................N......................................... .................. Pos Score Pred ------------------------ 7 0.187 - 15 0.507 Yes 24 0.247 - 33 0.297 - 106 0.486 - 138 0.558 Yes 177 0.863 Yes 272 0.473 - 293 0.787 Yes 336 0.605 Yes 477 0.554 Yes 602 0.548 Yes 633 0.677 Yes 658 0.265 - 681 0.668 Yes 728 0.285 - 742 0.085 - 758 0.728 Yes 784 0.156 - 793 0.175 - 812 0.650 Yes 820 0.262 - 939 0.640 Yes 992 0.660 Yes 1007 0.767 Yes 1014 0.377 - 1034 0.675 Yes 1144 0.097 - 1186 0.370 - 1305 0.411 - 1319 0.449 - 1367 0.556 Yes 1372 0.069 - 1377 0.290 - 1424 0.260 - 1479 0.067 -

Explain the output -- Processing locus 419: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCTTCTTTACATTTGGCAAACATGTTACTTATGGATGTTGTTAACGATGTGTTCTAAATAAGCTTCTGTTTCCTCTTG TTTTTCGTTTGTTTCTGATAGATATCAATGGAAAGGTCGTATAGCAATGCAAGAATGGCCAACAGTTTCACGTTCTGGAA TGATTGTGTTGAGCCAGAGGATTTAGAAGAAATGTGGATGGATCCTGCGGTCAGTGCTGAATGGATTGATGTTGGAGAGA CTAAAGGCCAGAAGGTTCACCTCTCAAGGGATCCTGATGGTCAGCCTTATCTTACACAGACCGAGATGAGGGCTGTTTCT GATATAACTGTCCGGAGGCATTTTGATTCCATCTTGGATTCGGAAATGATATGTGCAATTGCTGAGCTTGAAAGTGACCG AAAGCCTCTCATTATGCGGTACAGCAAAAAGACCAAAGAAACTGGCCTTGGAATTTTACAAGTTTTCGAAAAGACAGCAG CATGGTTGGCTGGGGGCCAAGGCTATCAGGCATATAATGTAGATGACAATCCTGATCTTCTTCACAAGCCCTTTATAAAT GTGTACTTTGGGGCAGCTTACCTGAAATGGTTAACAGACTATCAGAATAACCAGAGAAGTGAAGAGTTTGTTGTCAGAGC ATACAATGGTGGGACAAAAAAGGCGACTCACAAGTCGACATTGCCATATTGGAAACGATACCTTGCAGTGAAAGAAAGTC TCCCTTCAAGAAAACATGGCGATGCTGGCCCTTCAAGCTTTCGTCCTACGAATCCCGCTTCCCCAGGCTCAAACACCGAT TTCACATACTGGGACTCTCGAGCTTCCCCCGAAGATATGGAAGATATGTGGAATCAGTCTGAAATATGTAAAGAGTGGAC AAAATCTAAAGAAGAAAGGGGAAAGGTTCGTTTTTCACAAGATGGTGAAAAGAGGCCTTACCTTTCTCGAGGGGAACTTA AGGCTGTTGCAGAAATAATTGTTTCAAAGTACTTCAGCACGAAAGGAATTAGAGTTCCTCTTGTTTGTGCGATTGCTGAT ACTGTGTGCATGCGGTTTGTGAACGGTATTAAGAAACATGTAGGAATACTCGGAGTGGACTACTCAACCGCTTCATGGTT ATACTCGGAGCTAGGATACCGAGCATACAGAGTCGATTCAGCAGACGATCTAACCAAACCATTTATCTCTATGTACTTTG GTGTAGCCTATTTGGTTTGGTTATCGGAATATGAAGGAAGTCAAAGAAGTAATCAATTCATTGTCCAAGCATATATGAAA GGTCCTGACCACGTCGATCTTGAGGAATCATGTCCACTCTGGCTCAAGTTCGAGCAAGCTCTGAGTTACTATGAAGAGTC CAAGAGGGATTCAGGAAGCTGCGTTATCCTATGAGACAACGTTTATTAAGACACATATGATGAAGATTAACGGATTTAGC TTTGGTTTCGTGTGTTTGTTATCACAAGATTTCCTTTCTGGTTTCTGTTTTTG .......................N........N...N...........i............................... ...........................i..................N.......i........................N ...............................i.....i......................N.......N........... ....................................N............................i.............. .............................................N....N............................. .............i.................................................................. ..........................N..................................................... .....i.......................................................................... ...............i.....i.......................................................... ....................................i........i...................N.............. .........................................N...................................... ................................................................................ .........N...........................i....................................N..... ......................................................................N......... ..............................N...........................................i..... .............................N........................................N......... ..............................N.........................N..N.................... ..................................................... Pos Score Pred ------------------------ 24 0.259 - 33 0.350 - 37 0.296 - 49 0.604 Yes 108 0.790 Yes 127 0.409 - 135 0.616 Yes 160 0.382 - 192 0.713 Yes 198 0.833 Yes 221 0.153 - 229 0.087 - 277 0.125 - 306 0.800 Yes 366 0.384 - 371 0.306 - 414 0.609 Yes 482 0.265 - 517 0.388 - 523 0.260 - 559 0.361 - 587 0.498 - 646 0.521 Yes 736 0.706 Yes 742 0.614 Yes 837 0.806 Yes 846 0.600 Yes 866 0.245 - 922 0.155 - 1050 0.483 - 1078 0.715 Yes 1115 0.239 - 1191 0.399 - 1231 0.452 - 1275 0.615 Yes 1310 0.247 - 1351 0.347 - 1391 0.107 - 1417 0.069 - 1420 0.135 -

Explain the output -- Processing locus 420: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATTTGCAGCATGGCAAAAAGTGAAAAAAAGATTACAAGAAGAGAAAGGAATCGTTTGTACTGTGTATGTTGATGCAAAG GTGAGCTATTGCTTTCCTTAAACGTTAAAAGAACATCTTTTCGGATCAATTAAGAAACTTTATCAAATAAAAAATACTTT ATATTTTAATACCATATCGAGAACGATACTTCCTCTGCAACACGCCTACAATACGCCAACGAATATTTATATCATCAAAC CACTCTATTGTTTTCATTTTTCAACAAAATTCATCTTTAATTTTTCGATCCAAGGAGCTTCTCTGATCCTAATTAACCTA AACAAAACATTCATTACAGGTATCTTAAAGATTATGGAGGAAGAAGACAAGAAAGTGATGAAGTGTTTTAGCTTCAAGAG AATCATGAAGAGGAAGAAGAAAGAGGAGGAGGAGGAGAAGTTGATAGTGACTAGAGAATTCGCGAAGAGATGGCGAGATC TGAGCGGTCAAAACCATTGGAAAGGGATGTTACAGCCGTTGGATCAAGATCTCCGGGAATATATCATACATTATGGTGAG ATGGCTCAGGCTGGTTACGATACTTTCAATATCAACACCGAATCTCAGTTCGCCGGTGCTAGCATCTACTCTAGAAAAGA CTTCTTCGCCAAGGTTGGTTTAGAGATAGCACACCCGTACACAAAGTACAAAGTGACGAAGTTTATATACGCGACATCCG ATATCCACGTGCCTGAGTCATTCTTATTGTTCCCAATTTCACGTGAGGGATGGTCAAAGGAATCAAATTGGATGGGTTAC GTGGCGGTAACAGACGACCAAGGCACGGCACTTCTTGGTCGGAGAGATATCGTGGTTTCTTGGAGAGGCTCAGTGCAACC ACTTGAATGGGTCGAAGACTTCGAGTTTGGTTTAGTCAACGCCATAAAAATCTTCGGTGAAAGAAACGATCAAGTACAAA TTCATCAAGGTTGGTATTCAATCTACATGTCTCAAGATGAACGATCACCTTTTACTAAGACCAATGCTCGTGACCAGGTA TTGCGAGAGGTTGGAAGATTGTTGGAGAAGTATAAGGACGAAGAAGTTAGTATAACTATATGTGGTCATAGTCTTGGAGC TGCACTCGCGACGCTTAGTGCCACTGATATTGTGGCTAATGGTTATAACCGACCAAAGAGCCGTCCTGACAAGTCTTGTC CTGTTACTGCCTTTGTCTTTGCTAGTCCTCGTGTTGGGGATTCAGACTTTAGGAAACTCTTTTCCGGATTGGAAGATATC CGAGTGTTACGGACAAGGAATCTACCGGACGTAATTCCGATCTATCCACCGATAGGTTACTCCGAGGTAGGAGATGAGTT TCCAATAGACACAAGAAAGTCACCATACATGAAATCTCCTGGAAACCTCGCAACCTTTCACTGCTTAGAGGGTTACTTGC ATGGCGTTGCCGGGACTCAAGGAACGAACAAAGCTGACTTATTCAGACTCGATGTGGAAAGAGCCATCGGATTGGTCAAT AAATCAGTGGATGGGTTAAAAGACGAGTGTATGGTCCCAGGGAAATGGAGGGTTCTTAAAAACAAAGGTATGGCCCAACA AGACGATGGATCTTGGGAGTTAGTGGACCATGAGATTGATGATAATGAAGATTTGGATTTCTGATTTCTTGGTCATTTTT TCTTTGTCAAGTGTTGATGTTTGGAAACATACTATTTTATCTATTTAACGTTGTATTTTATGATAATTTATTCAATACGG CTA ..........N.......................................................N.....N....... ................................................................................ ................................................................................ ................................................................................ .................................i.......................i...................... ....N................................................................N.......... ..........................i.............................................i....... i............................................................................... ................................................................................ .................................................i.....................i........ ................................................................................ ......N......................................................................... ..........................i.........N..........................N................ ...........................................................N.................... ......................................N......................................... ................................................................................ .........................................................................N...... ............................i................................................... N..................................................N............................ ..........N...................i.............N........................i.......... .....N.......................N........N.....N................................... ................N..........................................N.................... ... Pos Score Pred ------------------------ 11 0.225 - 67 0.181 - 73 0.095 - 354 0.554 Yes 378 0.559 Yes 405 0.262 - 470 0.494 - 507 0.621 Yes 553 0.647 Yes 561 0.533 Yes 770 0.606 Yes 792 0.681 Yes 887 0.192 - 987 0.562 Yes 997 0.362 - 1024 0.460 - 1100 0.226 - 1159 0.199 - 1354 0.470 - 1389 0.630 Yes 1441 0.422 - 1492 0.190 - 1531 0.443 - 1551 0.610 Yes 1565 0.230 - 1590 0.643 Yes 1606 0.365 - 1630 0.122 - 1639 0.103 - 1645 0.285 - 1697 0.084 - 1740 0.043 -

Explain the output -- Processing locus 421: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATATATATAAAAGTATATTTAGGAGATATTCATGTGAATTAATGTGTGCTTTACTCTGATTTGAGTTTGCTTAACGTTT TTCCAAAATTTAAATAGGTAGCATTTTTTAAGTAATAAACAAAAGAATGAATTGGTCTTACTTTATATTAATGGATTCTT GATTAAGTTTCACCTGTTTTTTCACTTTGGAGCAACAATAATGGCAGCGTTTATCAGCGTTGACTTTCAGAACTGTTTCA TCTTCATTCTCCTATGCTTCTTCTCCCTCCTCTGTTATTCTCTCTTCTTCAAGAAACCAAAGGACTCACGAGGCTGTGAT CTACCTCCCAGCCCTCCGTCTCTTCCGATTATCGGTCATCTTCACCTTCTCCTCTCTTCTCTATCCCACAAGTCTTTACA GAAAATCTCCTCTAAGTATGGACCTCTCCTCCATCTCCGGATCTTTAATGTCCCCATCATCCTCGTTTCATCGGCTTCAG TTGCAGACGAGGTCTTCAGGATCCACGACATGAACATCTCCTCTCGCGGTGCTGCTGCGATCGATGAGTCCCTCGTGTTT GGATCTTCTGGTGTCGTCTACGCTCCCTATGGAGATTACTTGAAGTTCGTGAAGAAGATCATTGCCACTAAGCTGCTTCG ACCTCAGGTGTTGGAGCGGTCACGAGGTCTTCGTGCTGAAGAGCTACAACGACTTTACAACAGAATTCTTGATAAGGCGA AGATGAATGAGAACGTTGAGATCGGTAAGGAAGCGACGATGCTCATGAACAACATCTTCTGTAGGATGAGCATGGGAAGG AGTTTCTCAGAGGAGAATGGTGAGGCAGAGAGAGTCAGAGGCTTGGTGGCTGAATCATATGCCTTGGCGAAGAAGATTTT CTTCGCATCTGTACTGCGCAGACTGCTTAAGAAGCTTCGGATCCCACTATTCAAGAAGGATATCATGGATGTTTCCAACA GATTCAATGAGCTGCTAGAGAAGATTCTTGTGGAACACAATGAGAAACTGGACGAGGAACATAAAGACACGGATATGATG GACGTGTTGTTGGCAGCTTATGCAGATGAAAACGCTGAGTATAAGATTACTAGGAATCATATCAAGTCATTTTTCGTGGA GCTTTTCGTGGGAGGCACTGATACCTCGGTGCAAACAACACAATGGACAATGGCCGAGATCATTAATAACTCTGACGTTC TTGAGAGGCTGAGAGAAGAAATTGACTCTGTTGTAGGGACATCAAGAATGATTCAAGAAACAGATATACCAAACCTTCCT TATTTGCAAGCGGTGGTTAAGGAAGGACTAAGATTGCACCCACCATTTCCTCTCTTGACGAGGAAGTTTGAAGAAAGGTG TGAGATCAAAGGGTTCTACATCCCGGAGAAGACATTTCTTATTATAAATGCTTACGCTTGGATGAGAGATCCTGATTCCT GGGAAGATCCTAATGAGTTTAAGCCGGAGAGGTTTTTAGGTTCTTCAAGATTAGGACAAGTAGACGAGAGAGAGGAAGCT CAGAAGTACATTCCTTTCGGCGGAGGAAGGAGAGGCTGTCCTGGAGCAAATCTAGCTTCTATTTTCGTAGGAACCGCGAT TGGAGTGATGGTGCAGTGCTTTGACTGGGGAATCAAAGGAGATAAGATCAACATGGAAGAGACTTTTGAAGGACTGACCC TTACCATGGTTCATCCAATTAAGTGCACTCCGATTCCTCGAACACTGCCTTTTGTAACTTCTAATCTCCAGAGTCCTAGC TCTTGACACCTAACTCAGGAACCTGTAAGAAGTTTGCTTTCTTCATGTTTGTTGTATTGAAAGTCTAACATTCAGATCAA AGAAACTTTATATTCCACCACTAAT ................................N.........N..................................... ..............................................N.......................N......... ........................................i....................................... .............N.................................................................. ................................................................................ .................i.............................N................................ .............................i.................................N................ ............................N................................................... ................................................................................ ..i...N...............................N.....i....................N.....N........ ................N.........................................N..................... ................................................................i...N........... ......N................................N..................................i..i.. ...................N.....N...................................................... ..........................................i......i.............................. ...............................................N................................ ................................................................................ ...............................................i.............i.................. ............N................................................................... ................................................................................ .......N............................................i........................... .....i.......................................................................... ............................................N................................... ......................... Pos Score Pred ------------------------ 33 0.337 - 43 0.227 - 127 0.243 - 151 0.237 - 201 0.500 Yes 254 0.429 - 418 0.515 Yes 448 0.288 - 510 0.559 Yes 544 0.285 - 589 0.163 - 723 0.749 Yes 727 0.470 - 759 0.442 - 765 0.547 Yes 786 0.278 - 792 0.267 - 817 0.256 - 859 0.178 - 945 0.719 Yes 949 0.298 - 967 0.174 - 1000 0.136 - 1035 0.623 Yes 1038 0.593 Yes 1060 0.147 - 1066 0.055 - 1163 0.529 Yes 1170 0.861 Yes 1248 0.409 - 1408 0.557 Yes 1422 0.616 Yes 1453 0.480 - 1608 0.356 - 1653 0.692 Yes 1686 0.517 Yes 1805 0.045 -

Explain the output -- Processing locus 422: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGTACCAATTCTGGGATTTGTAATTAGCTGAGTATTGAGCTCTAAATCTTGAAAAATTAGTGAGTTTAGTATTTTGCGAA AGACCCATTAACAGATTATGATTTGTGCGTTGTTTCTTGCCAAATACTTTTGAGAGGTTTATGAAACAGAGTAAAACCCT AGAATTCAATTCCTTTGCTCTGTTATGCAGTCTTCATTTCATCAAATTCAACACAGAAGATTCGATGAGTAGCTTCAACG GAGACTCCATGGATGATTTCAAAGGCCTCCGAATAACTCAGCTTGATGATGATGACGATGATGAAACTGCGGTGGAACCT ATAAATATGGATGAATTTGATGATGATGATGAGGAAGATGATGAAGATTATGAACCTGTGATGTTAGGTTTCGTTGAGAG TCCTAAATTCGCATGGTCAAATCTTCGTCAACTGTTCCCTAATTTAGCTGGTGGTGTTCCGGCATGGTTGGATCCAGTTA ATTTACCATCAGGGAAGTCAATTCTATGTGATCTATGTGAAGAACCTATGCAATTTGTACTTCAACTTTATGCTCCTTTA ACAGACAAGGAATCAGCTTTTCATCGGACATTGTTTCTCTTCATGTGTCCATCTATGTCTTGTCTCCTTCGTGATCAACA TGAACAATGGAAACGTGCCCCAGAGAAGGCTATGCGGAGTACGAAGGTTTTCCGTTGCCAATTGCCTCGAGCTAATCCTT TTTACTCGAGTGAGGCTCCAAAGCACGATGGAACAGACAAGCCATTGGGTCATGGAGCTCCGCTTTGCACTTGGTGTGGA ACATGGAAAGGAGATAAACTATGTAGCGGCTGCAAAAATGCTCGATACTGTTCACCGAAACACCAGGCCCTGCATTGGCG CTTGGGTCATAAAACCGAATGCCAACAGCTTCGAACGGTTAGCGAAACATCTGACTCAGGCCCTGTCAATAATGGGGTTG CTCCAACTGAAAAGCAGAAAGTTGCGAGTAAGAGTTTATGGAAAGAATTTGTATTGATCAATGAGGATGAAAGTGAGTAT GATACTGAGATGTCAGGAGATGATGAAGTAGCTAAACCTTTGGTCTCAAAGAGAGAAGTTGACGACCAAATGAAATCGCT AATGAACGATTTTGAGGGAGATGCTGATAAAAAGAATTGGGTTAATTTCCAGCAACGCGTAGACAAAGCCCCTGAACAAG TTCTGAGATATTCCCGGAGCTCTGGTGCTAAACCCCTTTGGCCAATAGCAAGCGGACGAGTCTCTAAATCTGAGCTTCCC AGTTGCAAATCTTGTGGTGGTCCTCGTTGTTTTGAATTTCAGGTGATGCCGCAGCTACTGTTCTTCTTCGGTGGGAAGAA CGAGAGGGAATCTCTCGATTGGGCAACAATCGTGGTGTACACTTGTGAGAACTCGTGTGACTCGAGCTTAAGTTACAAGG AAGAGTTTGTCTGGGTTCAACTATACAGTCAGACAACTTAGGATCCTCTATTTCCTTCCTGGCTGTTGCCTCCAAAGTTT TGTTCTTAACCGGATCGTACCGGACTTAAATATATTTGTAAACTTGGGTATGTTACTTGG ................................................................................ .................N..........................................N................... ........................N.......................................i............... ........i...N................................N..N..N.....N..N................... ......i...N........N..N..N..N........N..N........N..........i................... ............N..................................................N................ .........................N........N............N.....................N.......... ..........................................N...........N........................N ......N........................i................................................ ...........................i.......................i............................ ..i.................N................i.......................................... ..................N....................................................N........ .....................................N......................N.....N...........N. .........i.........N..N..............................................N.......... ................................................................................ .............................................i.................................. ................................................................................ ................................................................................ .................................................N.......... Pos Score Pred ------------------------ 98 0.302 - 141 0.324 - 185 0.245 - 225 0.752 Yes 249 0.853 Yes 253 0.188 - 286 0.088 - 289 0.107 - 292 0.144 - 298 0.095 - 301 0.086 - 327 0.741 Yes 331 0.158 - 340 0.148 - 343 0.118 - 346 0.187 - 349 0.130 - 358 0.117 - 361 0.120 - 370 0.061 - 381 0.549 Yes 413 0.311 - 464 0.268 - 506 0.392 - 515 0.250 - 528 0.213 - 550 0.211 - 603 0.264 - 615 0.420 - 640 0.487 - 647 0.472 - 672 0.551 Yes 748 0.635 Yes 772 0.613 Yes 803 0.502 Yes 821 0.485 - 838 0.614 Yes 899 0.194 - 952 0.305 - 998 0.234 - 1021 0.379 - 1027 0.410 - 1039 0.116 - 1050 0.790 Yes 1060 0.160 - 1063 0.129 - 1110 0.389 - 1122 0.514 Yes 1141 0.240 - 1326 0.629 Yes 1570 0.041 -

Explain the output -- Processing locus 423: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGATTTTGCATGCGTTTTGGTAGATTCTCTTTGGAGATTTTAGGTTATTGAGTTTTCTCAATCCACTAGATACTTGAAA TTGATTTGTGTTTCTTTGTAGCTTGTGGGTCTTTTGATTCTGTGTGGTTGTTTTGATTGTGTTGGATTTCTATTACTGAT GAACAAAGAAACAGTGTCTGCAATGTCTTGTTCTGCCAGGTTAAAAACCAATTTGGGGATGACTTCTGCTCTGAGTACTC ATGAGGTTCTTAACAGTAATGATACTATGTGTGAGATTCTTATCCTGCTTCCACCAGAAACGATATACAAGCTGATTTTG GTGTCCAAGAGATGGCTTGAGATTATAGCCAGCCCTTGCTTCCGTCACACTTACTTAGCCAAGTGGAAACCTAATTTTGA ACTCATTGGCTTCTTCGTCTGCAGCTCAATGTACCTTGGAAGACGCATAGATGGAACTCGTCGCCCTCGCGCTGAGCCAT CTCTTCCTTTGCTCTCAACCAGCAGTATTGGTGATGAAATTGAATCCTCTGGTATTCTGAAAAAGCTTGGATACTACATC GACTCTGCAGATGGTCTTCTACTCTGCGGCCGCCATCCAAAAGCTTACTACTTGTGGGATCCGAGTACACAGAAGCAGCA GCAACTTCCACGTCCCAGAGTTCATTTCGAAGAACTTTGTATGTCTTTGATTTCTGAGGATAGTCCTGATCAAGGGTTTA GCTACAAGGTGGTCCGGGCAGAGTGTGTAGCCTTTCCTGTTAACAGTACAAAGGTGAAGGTAGAGACTTTTTCCTCAAAA ACTTCTACGTGGAGCTACTCAGAGCTTATTTGTCTCGAGCCTCTATCTCTCAGCCCGTGGACTCCAGGGAGAGTGATTAA AGGCGTTGTTTATTGGCATGCTAGAGGAGGTAAAATTGCCATATATGACTCAAACAGTGAGGAGAAACGGATTGATGTGA TAAAGCTTCCGAAAACATATGACTATGATGAACAGGTTCTGGGAGAAACCGATGATGGGTCTTTACAATACGGATGGAGC AATAAGTCGGTAATGGAGGTATGGAAGCTTGAAAAGGTAGGCAATGTGCTTGAATGGAACCTGCTGTTTAAGGTGAATTT CAAGGCGATGTGGAAAATGAACCAAGCGGTAGCTACAAGATTCAGCACTTGGACTAAAGAAACGCAGCTGCTTGCGCTCT TCAATCAGAAGTCGGACTTGGTGTACATCAGATGTGATACACAAATCTTCATCTATCACACAGAGACTAAAAGGGTCGAA GTAGTTCAGTACCAAGGACGAAAATCGACACTCGTTTGGGATTTCAACAAAGTGGTGCCTTACTTTCGACGTACCTGGCC TTGTTCTCCTCTGTTTGAAAACAATGCCATGATGATCGACACTCCTCAATGCAACGCCTCTTCCGCAGGAAGTAACTAAG TAGTTTATGCTTTTAATCAGTTTATGTATGTAGGCTATTCAACTATCTTTTATGTTTTGATGCTGAAGGTTTTTGGTTTT GGCCATTGATGAACCTCT ..........N..................................................................... ..............................................................................N. ......................i...................................i..................... N.................N.......i..................................................... ...........i.................................................................... ............................i.....................N............................. .................................N.............................................. ..........N..................................................................... ........................................i....................................... ................................................................................ ................................................................................ .................N..........................N.............................N..... ..................N.....N..N.......................N..N..................i...... ............N.......i......................N.........i.......................... .......N........N............................................................... ...............................N................................................ ................................................................................ .......................N....N..N................N............................... ......N................N...N.......................N.......N.................... ........N......... Pos Score Pred ------------------------ 11 0.233 - 159 0.349 - 183 0.689 Yes 219 0.643 Yes 241 0.108 - 259 0.211 - 267 0.535 Yes 332 0.576 Yes 429 0.546 Yes 451 0.467 - 514 0.254 - 571 0.322 - 681 0.747 Yes 898 0.210 - 925 0.259 - 955 0.213 - 979 0.286 - 985 0.288 - 988 0.313 - 1012 0.178 - 1015 0.205 - 1034 0.685 Yes 1053 0.484 - 1061 0.586 Yes 1084 0.178 - 1094 0.678 Yes 1128 0.237 - 1137 0.345 - 1232 0.354 - 1384 0.495 - 1389 0.240 - 1392 0.419 - 1409 0.274 - 1447 0.066 - 1464 0.077 - 1468 0.041 - 1492 0.079 - 1500 0.047 - 1529 0.036 -

Explain the output -- Processing locus 424: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTCATCATCACCGATCCACTTTCTGATGTAAGAAAACTACAGTCTTCCTTCCTTCTCACTTCTCTTCTTATCTTGCTT TCATGGTTAGGGCAAGTTTGATTTAAGCTTATGTGGATGATAGAACTAGTGTTTATACTTGTAGTTAGGTCAATGGAACA GACAAAGGATGATTCTGATTTTGAGGATGATTGTGAAACGAAGAAGATGGAGACTGATACATCAGCTCAAGAGGCAAGGA ATGGTAAAGACATACAAGGGATTCAGTGGGAAGGGTTTAAGTATAATAGAGACGAGTTTCGTGAGACTAGGTTGAAACAA TATGATAACTTTGTTAATATCTTAAGGCCTAGCTCTCGAGAAAAGCTTGATAAGGAACATGGGCAAGTAGTTGAGAAAGG AAAGAACTTTTATGACTTCCAATTCAATACAAGGCTTGTCACTTCCACTATTGTGCATTTTCAGCTTCGGAATTTGGTAT GGGCGACATCTAAGCATGATGTATATCTAATGCAAAATTATTCACTCATGCACTGGTCATCTCTACTCCAGAGAGGGAAA GAAGTACTTAATGTCGCCAGATCAGTTACCCCCACTCAGAAACTACCTGGATTGTTTTCTGAGCCACTCTCTAGGGTGCA AGTTAGCAGCATGGCAGTCAAAGAAAATCTGATCCTTTTAGGAGGGTTCGACGGGGAGCTTCTCTGCAAGTGTGTAAATC AGCCTGGGGTTGCTTTTTGCACAAGATTATCAACGGAAGATAATGCCATCACAAACACAGTTGATATATACCGAGACCCA AGTGGCTCCCTTAGGCTTATAACCGCAAATAACGACTGTAAAATTCGTGTATTCGATGCTCAGAGCTTTACACGTGTCAG TGAATTTGCTTTAGATTGGTCTGTCAATAATACCTCAGTTAGCCCGGATGGGAAGCTACTCGCTGTAGTCGGGGACAGTA CAGAATGCTTGATCTCTGATTCCCATTCTGGAAAAGTTATTTCAAGCCTCAGAGGCCACAAAGACTACTCATTTGCATCT GCTTGGCACCCGAATGGTCTAATCTTAGCAACGGGAAACCAAGACACGGCATGCCGTCTCTGGGACATTCGTAACCCGTC AGAATCATTCGCTGTCCTGAAAGGAAACATGGGAGCCATCAGAGGACTGAAGTTCACACCAGAAGGGCGGTTTCTTGCAA TGGCTGAGCCTGCAGACTTTGTTCACATCTTCGACACGCAGTCCGGGTTTCTACAGTCCCAAGAGATTGATCTGTTTGGA GAAATAGCCGGGATCTCGTTCAGCCCCGATACAGAGGCACTATATGTAGGGGTCGCAGACCGCACTTATGGAAGTTTGCT GGAGTATAAGAGGAGAAAGGATAATCACTATATGGACTCCTTTTACTGAGAAAAGGTAAATGAAAAGTAAAAAGCATCTA ATATGTTGTCACAAGATGGGTTTAACCCTATTAGTGTGTATATGTAGACAGAATTGGTAGTTTCTTTG ...........................N.................................................... ..i...........................i.....i...................................i....... ........N.................N...................i................................. N............................................................................... ...........N..................................................................i. ...............N..N..........N.................N................................ ..........N..................................................................... ..........i..................................................................... ..........................................N..................................... .......................................................N........................ ...............................................N................................ ....N........................................................................... .............N....................................i............................. ............................i..................................................i ................................................................................ ...........................................N.......................N............ ...............................N...........................N.................... ..N............N.........................N.......................... Pos Score Pred ------------------------ 28 0.469 - 83 0.527 Yes 111 0.733 Yes 117 0.591 Yes 153 0.683 Yes 169 0.281 - 187 0.301 - 207 0.792 Yes 241 0.129 - 322 0.209 - 379 0.267 - 412 0.185 - 479 0.817 Yes 496 0.213 - 499 0.293 - 510 0.255 - 528 0.403 - 571 0.322 - 651 0.675 Yes 763 0.246 - 856 0.232 - 928 0.442 - 965 0.274 - 1054 0.493 - 1091 0.657 Yes 1149 0.769 Yes 1200 0.671 Yes 1324 0.228 - 1348 0.172 - 1392 0.269 - 1420 0.050 - 1443 0.171 - 1456 0.045 - 1482 0.054 -

Explain the output -- Processing locus 425: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCATGTTTGTGATATTCATGGATTGATTATGCCCAAGCATAAAGATGAATACTTTATTTCATTTTACGAGACTGTGTGTA TATTATTAGTGTGTTCATTAGCTGACTACCAACTGTTCGATGAAATGTCTCAGTCAAATGGACTCTCTTCCTGATGCGAT CCTTCAATACATTCTTTCCTACCTGACTTCTGCAAGGGATGTGGCTGCTTGCAACTGTGTCTCCAAGCGATGGAAAGAGT CTACAGACTCTGTTAAGAGCGTTGTTTTCCACAGGAATTCATTCGAAAGCATCATGGAGACTGATGATTCTGACTCCATC GTTCGGAAGATGATCTCTTCTTCTCGTCGTCTTGAGGAGCTTGTGGTGTATAGCCCATTCACTAGCAGCGGCCTAGCATC TTGGATGATGCATGTGAGTTCATCTCTAAGGCTATTGGAGTTAAGAATGGATAATCTCGCTAGCGAGGAGGTTGTTGTCG AAGGCCCTTTGAAGCTTGATTGCATTGGTGTGGCCAAGAATTTGGAGATTTTGAAGCTTTGGGGTGTTTTAATGATGAGC CCTCCTAAGTGGGATATGTTCCCAAACCTTCGAAGCCTCGAAATAGTGGGAGCAAAAATGGATGATTCCTCACTGTCTCA TGCTCTTCGTGCATGCCCGAATCTGAGTAACTTGCTTCTACTAGCTTGTGAGGGAGTTAAATCCATATCAATCGATCTGC CATACTTGGAGCACTGTAAGCTGGATTTCTATGGACAAGGTAACACCTTGCTAGTCCTCACGAGTCAGAGATTAGTGTCC CTTGATGTTCAAGGTTGTAGCTGGATTCGTGTCCCTGAAACCAAATTCTTAAAGAACCTATCCATCTCCAGTGTTACTGG GAGAGTTTACATGGTAGATTTCAATAATCTTTCATCGCTTGAGGCCTTGTCGATTCGAGGTGTACAATGGTGTTGGGATG CAATATGCATGATACTGCAGCAAGCAAGAGACGTGAAGCATCTCTTTATGAAGGTTGAATTCACAGGCAATGAGGCTCTT CAACCTTTCCCTGAGATTGATTTTGTCGAGTTCTTCAATAACCATCCAAAGCTTCAAACATTCGACATCCATGGAGCAAT GTTCGCTGCACTCTGCCAGAAAAACAGCCTTAAGAAGCTTGAGACCGGATTTACAATACCGTGTTTGGAGGAGGTTGTTA TCACAGTGAGATCTCCACTGAACGCAGAACAGAAGATGAACACGCTTGAATCACTAGTGAAATACGCGAGAGGTCTGAAA CGAATGGTGATAAGGATTCTCCGGATGAAGAGCAATCACAGCAGTGCAGATGATTTCTGCGATGATATCTGCAAGTTCCG GCACATGAACGAGCATCTTGTTCATATAGAATGAAATAATTTTTTGCTGCTAATCTTATTTTCAGCCATTTGATTTCTGT TTCTCTTTCTGGTCGCTGAGCATTTCTTATATTGAACTCTTGAGTAATTGTTG ..N..............N..........N...............N................................... .......................................i....i............i...............N...... ......................................N..............................N.......... .....................................................i.........N................ .........N...................................................................... ....N..i...N..................................i................................. .......................................................................N..N..... ...............N.........................................i...N.................N ............N................................................................... ..............................N................................................. ....N........................................................................... ..........i.......................................................i..........N.. ....N...i......................................i.....................N.......... ......................................................................N.......N. ................................................................................ ...................................N............................................ ...N....................N........................N...........N.................. ....N.........................N................................................. ..................................................... Pos Score Pred ------------------------ 3 0.119 - 18 0.171 - 29 0.382 - 45 0.493 - 120 0.573 Yes 125 0.696 Yes 138 0.536 Yes 154 0.313 - 199 0.271 - 230 0.407 - 294 0.730 Yes 304 0.117 - 330 0.434 - 405 0.388 - 408 0.833 Yes 412 0.214 - 447 0.786 Yes 552 0.301 - 555 0.307 - 576 0.287 - 618 0.881 Yes 622 0.422 - 640 0.147 - 653 0.248 - 751 0.465 - 805 0.395 - 891 0.690 Yes 947 0.739 Yes 958 0.088 - 965 0.234 - 969 0.607 Yes 1008 0.521 Yes 1030 0.218 - 1111 0.177 - 1119 0.478 - 1236 0.364 - 1284 0.287 - 1305 0.128 - 1330 0.185 - 1342 0.089 - 1365 0.203 - 1391 0.281 -

Explain the output -- Processing locus 426: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTTCAGCTAACTCAAACAAAAGTTAGTTGGCCTAAGTTTTTCTTTTTGTTATTTTTTTAGTCAATTCCCTATTGAATA ACTCCAAAAATGCTGTCATAAATTATTATTTTTGTTTTATGTACTTGTATTCTGTTTTGTCTCAATTATTTCTCCAATCA GACAAAGAATAGCAGTTTAAAGTCAATATTTATGTTCACAGGTTATAGTTTTGGTTTTGGGGATAGTAGTGAAGAAGAAG TTGCACAACACACATTCTTTCCCTTTTTACTTGTCTCTCTCTCCCCACAAGAGTATCTTCTTTGTAGGGGATTGTCAACG CTCTTCGATAGAGGTGCCCAAGAAGCTTCTCTTCTCTCTCTCTCTCTCTCTTCAATGCCCTCCTCTGCTACTAGCACATA TCTCCTCTCTTTAAAACAAGTCTCTCTCACAGCTTCTTCACCACTCCTTCTCCAGCTCAAAGACCGTTTCTTTCACCAGC CAAAGCTCCTTAACATGGGATTCTCAACTGGGAAAAGAAAATCAAGAGATGAAGAAGAAGATAGAGTGAGCTTCTTCGCT TCTGAATTCCCCATGGATGACCTCAACGATGACGTTCTCGAGAGAGTCCTCTCATGGCTACCAACTTCTTGCTTCTTTCG TATGAGCTCTGTTTGCAAAAGATGGAAATCAAGCCAAACCTCGAAAAGCTTCAAGCTTGCTTGCTCTCAAATCCCTACTC GAGATCCTTGGTTCTTCATGATCGACAACGATTCCAATTCTTCCTCCTTCGTTTTCGACTCCACTGAGAACTCCTGGAAA AATCTCAACCGCCGCGATTTCCTCCACCACCACCGTCAAGATTTCATCCCCGTCGCTTCTTCCGGCGGCTTGCTCTGTTA CCGCTGCTCCATCTCCGGCGACTTCCTCCTCCGTAATCCACTCACTGGATCTTCTCGTGACATTCCTTCTCAAGATAACA ACAACAACAAACCTCTCCAAGCCGTAGCCATGACCACCACTACTGTTACTCCCTCCAGCTATACGCTCGTTACCATCTCC GGTGAAATCCCAAATCTCAGTTTCAAGATCTACGAATCAAACGCAGATTCTTGGAGCAAAGACCAAGAACTGGAATCAGT CAAGAACAATGATTCATCATTACATGATGATTATGATACTGACAGTGGCACAGTCTACTTTCTTAGCAAACAAGGAAACG TTGTCGTGGCGAGTAACAATCTTCAGAGAAGTCCGTCGAAGCAATACTCTTCCGTTATTACCGTTACAGATGAAGCCGAA ATCGTCTATTTTCTCAGCTCTTACGGAACAATTGTAGCTTGTGATCTCACTAAACGATGCTTCACCGAGCTACCAAAGCT TCTTCTTCCGTTCCTAGAGTACTCCATCGACTTGGTGGAATGTGAAGGAACAATGTATGTGATTCTTCTCTCTGAGTTCT TTGAAAGCGCAAGTTTAAGGATCTGGAGACTTGACAATAACCGGGAATGGGTTCAAGTCGGGATGTTGCCTCCCGCGTTG TCTCATGAGTTATACGGTAAGAAAGGCGACATAAACTGCGTTGGAGGAGCTGGAAACAAGATACTCGTGTGCTTCAATGC TAGTCCTCCAGAAGTGTATTGTAGATACTTTGTGTATGATTTAGTTGCAGAAGAGTGGAACGAGTTGCCGAAATGCTTCA AGGATGGAGAAGCTGTGGATTTTGTTTCCGCTCTTTCTTTCCAGCCTAGGATCGAGGCTACAGTTTGAAATTTTTTAGCT TTTATTGTATGGATTGTGTGTATTCTTTCTATTCAGGAAAATGCAATGTAACTTGATTTGTAATCTGTTGACATTGGTTA GAACAGG ................................................................................ .........N............................N......................................... ...............................N................................................ ................................................................................ ......................................................N......................... ................................................................................ ..............i.................................i............................... ............i...N...........i........................i.......................... .................N.............................................................. ................................................................................ ................................................................................ .............................i.................................................. ................................................................................ ........i..............N..N.....N............................................... .....................................................................N.......... ........................................................N....................... .......................................i............N...N....................... ..............................................i...............N................. ....N.......................................................................N... ...................................N....................................N....... ...N............................................................................ ........N...............................N....N.................................. ....... Pos Score Pred ------------------------ 90 0.304 - 119 0.306 - 192 0.299 - 375 0.354 - 495 0.789 Yes 529 0.594 Yes 573 0.528 Yes 577 0.411 - 589 0.558 Yes 614 0.522 Yes 642 0.253 - 662 0.441 - 738 0.487 - 990 0.790 Yes 1129 0.654 Yes 1144 0.181 - 1147 0.341 - 1153 0.350 - 1270 0.407 - 1337 0.327 - 1400 0.678 Yes 1413 0.474 - 1417 0.356 - 1487 0.611 Yes 1503 0.440 - 1525 0.320 - 1597 0.320 - 1636 0.222 - 1673 0.239 - 1684 0.144 - 1769 0.101 - 1801 0.079 - 1806 0.064 -

Explain the output -- Processing locus 427: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTTTAATCTGTTTTTATCCATTTGACTATTAAAGTTTTGAAATCTGTTAGTGTTTTTGAATTGGGTTTTGCAGATTTCT TGATTTTTATTTATAGATTAGGGTTCTGATACTAAAGTTTGGATTTTTAAACTAAAGTTTGGATTTTTTTTTGTATTCAT GGTTCAGATCCAGTTATTAGGTGGAAGCAGATTTTGTAGAAAAATGGATGGTAGTATGAATTTGGGGAATGAGCCACCAG GAGATGGTGGTGGAGGTGGAGGGTTGACTAGACAAGGTTCGATATACTCGTTGACGTTTGATGAGTTTCAGAGCAGTGTA GGGAAAGATTTTGGGTCAATGAACATGGATGAGTTGTTAAAGAATATATGGAGTGCTGAAGAAACACAAGCCATGGCTAG TGGTGTGGTTCCAGTTCTTGGTGGAGGTCAAGAGGGTTTGCAGCTGCAGAGGCAAGGCTCGTTGACTCTGCCTCGAACGC TTAGTCAGAAGACGGTTGATCAAGTTTGGAAAGATCTATCCAAAGTTGGAAGTAGTGGAGTAGGGGGAAGTAACTTGTCT CAGGTGGCTCAGGCTCAGAGTCAGAGTCAGAGTCAGAGGCAGCAAACATTAGGTGAAGTAACTTTGGAGGAGTTTTTGGT TCGTGCTGGTGTTGTGAGAGAGGAAGCTCAGGTTGCTGCAAGAGCTCAGATTGCTGAGAACAATAAAGGCGGTTACTTTG GTAATGATGCCAACACAGGTTTCTCTGTCGAGTTTCAGCAGCCTTCTCCACGAGTTGTTGCCGCTGGTGTAATGGGAAAT CTTGGTGCAGAGACTGCAAATTCTTTGCAGGTTCAAGGTTCTAGTTTGCCTCTGAATGTGAATGGAGCTAGAACAACATA CCAGCAATCGCAACAGCAACAGCCAATCATGCCTAAGCAGCCTGGTTTTGGTTATGGAACACAAATGGGTCAGCTTAATA GTCCTGGGATAAGAGGTGGTGGTCTTGTGGGACTTGGAGATCAGTCTTTAACGAACAATGTGGGCTTTGTCCAAGGTGCT TCTGCTGCAATTCCTGGAGCTTTAGGCGTTGGTGCTGTGTCGCCTGTTACGCCATTGTCATCAGAAGGGATAGGGAAGAG TAATGGTGATTCTTCATCACTCTCTCCGTCTCCTTACATGTTTAATGGTGGTGTGAGAGGTAGAAAGAGTGGCACTGTGG AGAAAGTTGTAGAGAGAAGGCAAAGGAGAATGATAAAGAACCGAGAATCAGCTGCAAGGTCCCGGGCCAGGAAACAGGCT TACACCGTGGAGCTTGAAGCTGAAGTTGCAAAGTTAAAGGAAGAGAATGACGAGTTACAACGAAAGCAGGCAAGGATCAT GGAAATGCAAAAGAATCAGGAGACGGAGATGAGGAATCTTCTGCAAGGAGGTCCAAAGAAAAAGCTGAGGAGGACAGAGT CGGGACCTTGGTGAATCAATCAATGCCATCATACTTAGTTTCTGTAGATAAATGACATCCCACTTAGGTGTTTTAGTTGA ATTAGACTTAATAGAGAAGAGCTTTCATCGTTT ................................................................................ ..............................................................................i. ...........................................i...N.......N............i........... ...N........................................................N................... ..................i.....i...N..................N........................i....... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ...N..N................................................................i........ .......................................................N.....N.................. ............................i........................N..........i............... .........................................................N...................... ................................................................................ ..i..................................i......i................................... .............................i.................................................. ..............................................N...............................N. ....N.......................N................................................... ......................N............................N............................ ................................. Pos Score Pred ------------------------ 159 0.679 Yes 204 0.792 Yes 208 0.341 - 216 0.293 - 229 0.562 Yes 244 0.216 - 301 0.099 - 339 0.614 Yes 345 0.731 Yes 349 0.315 - 368 0.480 - 393 0.627 Yes 724 0.165 - 727 0.185 - 792 0.685 Yes 856 0.286 - 862 0.200 - 909 0.603 Yes 934 0.343 - 945 0.548 Yes 1018 0.365 - 1123 0.674 Yes 1158 0.530 Yes 1165 0.561 Yes 1230 0.800 Yes 1327 0.203 - 1359 0.462 - 1365 0.289 - 1389 0.213 - 1463 0.096 - 1492 0.050 -

Explain the output -- Processing locus 428: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACATAAATATGTGATCAGAGAGAGTGATCTACTCTAGGGTTGATTCGCTTGACTTTTTTTGGTTTTTGAATTATTGGTG TTTGTGTGTTGGCTATGGTGATTTGATTTGGAATTAGGGTTCATGGCTACAAAGTTAGAGTCTTTTAAGGTGGTGGAGAA ATTGGGTGTTGAGAAAGGTGAAAAAGGGAAGATGATGAGTAAAAAAAAGAATGTTAAGAAAGATGGAGATGAGAGTGAAA GTGGGTTTTGGTTTAGATTCAAGTTTATCTTTAGTTGTATCTCTTCTAGATCAAAAGTTGATAGTTCCATGAATGCTACT GCTGTTATTGCAGAACCTAAGAAAGTTATTGAGAAGCTTGAAGGTCATCCAGCTCCGACTAAAGACACTGGCTGTGCGGA GAGCGGTTCCTCAACACCTCTAATGAGCGGGGAATTGAAGTATTCTTCTAAGCTACGGATTTTCATGTTTAACGACCTTA AGTTAGCCACTAGGAATTTCAGACCAGAGTCTCTTCTCGGTGAGGGTGGGTTTGGATGTGTTTTTAAAGGATGGATAGAG GAGAATGGAACTGCACCTGTCAAGCCTGGTACTGGTCTAACTGTAGCTGTCAAAACACTGAACCCAGATGGCCTTCAAGG TCACAAGGAGTGGCTGGCTGAAATTAACTTCCTTGGAAACCTTGTTCACCCCAGTCTGGTTAAATTAGTTGGCTACTGCA TGGAAGAAGACCAAAGGCTGCTTGTTTACGAGTTTATGCCACGAGGAAGTTTGGAGAACCATCTTTTCAGAAGGACCTTA CCTCTCCCTTGGTCTGTTCGAATGAAAATTGCACTTGGTGCAGCTAAAGGTCTCGCCTTTCTTCACGAAGAAGCTGAGAA GCCAGTCATATATCGAGATTTCAAAACATCTAATATTTTACTGGATGGGGAATATAATGCAAAGCTCTCTGATTTTGGGC TTGCAAAAGATGCTCCAGACGAGAAAAAATCCCACGTATCAACCCGAGTCATGGGAACTTATGGTTATGCTGCCCCTGAA TATGTAATGACTGGACATCTGACAACGAAGAGCGATGTATACAGTTTTGGAGTAGTTTTACTTGAAATATTAACTGGACG AAGATCTGTGGATAAAAGTCGGCCGAACGGGGAACAGAACTTGGTAGAATGGGTGAGACCTCATCTCTTAGACAAGAAAA GGTTTTACCGGCTATTAGATCCTCGGTTAGAGGGTCACTACTCGATCAAAGGTGCTCAGAAAGCCACACAAGTAGCGGCG CAATGCCTTAACCGAGACTCCAAAGCCAGACCGAAAATGAGTGAAGTTGTGGAAGCCTTAAAGCCATTACCAAATCTTAA AGACTTTGCTAGCTCTTCTTCTTCTTTCCAAACAATGCAGCCTGTTGCTAAGAACGGAGTCAGAACACAAGGAGGAGGGT TTGTGTCGAGAAATGGACCGCCAATGAGGAGTTTGTCTAGCTTAAACTTGCCTCAAGCATCCCCTTATCGTTATGCCCGT CAATCACCAAAGCCTAAAGGAAAAGAGCCATGACTCCAAAATAGATATTAATTTAGTTCTTCATGTCTGACCAAGGTTTA CTGATTTGAGCTGTATGTTCAGCTTTCTGTTTCATCGATCCTTTCCGACTCA .........N...................................................................... ..............N...........................i..................................... ...............................N..N...............N...........N.....N........... ....................................................................i...N....... ................................................................................ ......................i.........................................i............... .......................................................N..............i......... ....N..............................................................N............ ...............................................................................N ...................................N............................................ .....................i.......................................................... ............................................N...........N....................... .........N........................................i.........N.....N............. ................................................i............................... ................................................................................ ..N.................................i........................................... ..................................i............................................. ............i..........N................................................N....... .............................i................................N................. ..............N..................................... Pos Score Pred ------------------------ 10 0.479 - 95 0.346 - 123 0.767 Yes 192 0.323 - 195 0.178 - 211 0.274 - 223 0.122 - 229 0.096 - 309 0.865 Yes 313 0.240 - 423 0.620 Yes 465 0.563 Yes 536 0.161 - 551 0.509 Yes 565 0.366 - 628 0.274 - 720 0.456 - 756 0.335 - 822 0.533 Yes 925 0.326 - 937 0.450 - 970 0.329 - 1011 0.527 Yes 1021 0.213 - 1027 0.135 - 1042 0.277 - 1047 0.386 - 1075 0.292 - 1169 0.647 Yes 1283 0.200 - 1317 0.718 Yes 1395 0.577 Yes 1453 0.791 Yes 1464 0.273 - 1513 0.272 - 1550 0.599 Yes 1583 0.084 - 1615 0.036 -

Explain the output -- Processing locus 429: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGATTTGCTTCTGCTCTTTCTATCTCTCTTGGTGCTTCTATGTTTTTATCACCTTTTACGTGTTTGTTATGTGATCTCAG AGACAACGAGTTCGATTATAAATTGTAATCTGTTTTTTTTTTCTAATGTCGATTTCATATTTTTGTTATGCTTGTCCAGT CATGGCAGGAATGGCATCTGATGGAACTCAATATGATCCTCGTCAGTTTGATACTAAGATGAATGCCATTCTCGGCGAGG AAGGAGAGGAAACATTCTACACTAACTATGACGAAGTTTGCGACAGCTTTGATGCAATGGAATTGCAACCAGATCTTCTC AGGGGTATTTATGCTTACGGTTTCGAGAAGCCTTCTGCTATTCAGCAAAGAGGAATTATTCCATTCTGCAAGGGTCTTGA TGTGATCCAGCAAGCTCAATCTGGAACTGGAAAGACCGCTACCTTTTGCTCTGGTGTTTTACAGCAGCTCGATATTAGTT TGGTTCAATGCCAAGCTCTTGTTTTGGCACCTACCCGAGAACTTGCCCAGCAAATTGAAAAGGTTATGAGGGCACTTGGT GACTACCTTGGCGTCAAGGCTCAGGCTTGTGTTGGTGGAACAAGTGTTCGTGAGGATCAACGTGTTCTTCAGTCTGGTGT TCATGTTGTTGTTGGAACCCCTGGTCGTGTCTTTGACTTGTTACGCAGACAATCCCTCCGTGCTGATGCAATCAAAATGT TTGTTCTTGATGAAGCTGATGAAATGCTCTCTCGTGGTTTCAAGGATCAGATATACGACATCTTCCAGCTTCTTCCTTCC AAGGTTCAGGTTGGTGTTTTCTCTGCTACAATGCCTCCCGAAGCCCTTGAGATCACAAGGAAGTTCATGAACAAACCAGT CAGGATTCTGGTCAAGCGAGACGAGCTGACTCTTGAAGGTATCAAGCAGTTTTATGTCAATGTAGACAAGGAAGAGTGGA AACTCGAGACACTATGCGATCTCTACGAGACACTGGCCATCACTCAAAGCGTCATCTTTGTGAACACAAGGCGTAAGGTT GATTGGCTAACCGACAAAATGAGAAGCCGTGACCACACTGTTTCAGCTACACACGGAGACATGGACCAGAACACAAGAGA CATCATCATGCGCGAATTCCGCTCTGGGTCTTCCCGTGTCCTCATAACCACTGACCTCCTTGCTCGTGGTATCGATGTTC AGCAAGTCTCTCTGGTCATAAACTTTGACCTCCCAACCCAACCGGAGAATTACCTTCATCGTATTGGAAGAAGTGGAAGG TTTGGGAGAAAGGGAGTAGCAATCAATTTCATGACATCGGAGGATGAGAGAATGATGGCTGATATTCAGAGGTTTTACAA TGTGGTCGTTGAGGAGTTGCCAAGCAATGTTGCTGATCTTCTCTGAAGCCTTTCTTTTTTCTGTTTTTTACTGAGAAAGT GCTGTGTTTACCCCTTTTTTTGGCAGCTTCTCTATGCTTTGATTTTTGTTTTCAGGCTACTACTT .......................................N............................N........... .............................................i.....................N............ ...........................N.......................N....i....................... ..........N....................................................................N ................................................................................ .......N.........................................................i.............. ................................................................................ ..N..............................................................N..........i... .........N........N....i........................................................ ..............................i...................................N............. .....................................................N.....N.................... .............i.................................................................. ..................i.........................................i................... .......i..................................................................N..... ................................................................................ ..............................N............N.......N..i........................N ..........................N..................................................... .................................N............................... Pos Score Pred ------------------------ 40 0.141 - 69 0.351 - 126 0.807 Yes 148 0.317 - 162 0.766 Yes 171 0.805 Yes 181 0.521 Yes 193 0.241 - 219 0.670 Yes 223 0.308 - 268 0.400 - 292 0.144 - 297 0.657 Yes 331 0.260 - 400 0.305 - 488 0.450 - 546 0.575 Yes 643 0.134 - 706 0.205 - 717 0.728 Yes 730 0.109 - 739 0.166 - 744 0.845 Yes 831 0.534 Yes 867 0.493 - 934 0.211 - 940 0.200 - 974 0.562 Yes 1059 0.553 Yes 1101 0.779 Yes 1128 0.581 Yes 1195 0.238 - 1311 0.340 - 1324 0.083 - 1332 0.427 - 1335 0.703 Yes 1360 0.358 - 1387 0.277 - 1474 0.043 -

Explain the output -- Processing locus 430: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTCGGTTCTGTACATTTTGTTCGGTTTATTTCGGTTCGGTTCTTTTTGTTGTCAAGGTTCTTGAGAAAGACGAGTTGC TAAAACCTTGTTATGTTCTAGTTGGGCCGTGTTAATGGGCCTACTATTGAAAAGCCCTTGAATATGAAGAAAAATTACAA AAGTGCCATTAGTTCTTCATCTCCCTCACAACTCAGAAGAAGAAGAATTTCTCCGGCTAAAACCCTTTCTTCTATCATCT ATGCGAAAGCTTCATCTTCTTCAAAGAAAATGGTTTCAGTGTTATTCCAATCTTCATGTTCCTGTTCACTTTCTAGCTTT CGCTCTGTAAAATTCGGAGCCCGTTTAGTCTCTCCTCTGTATTTCGCCGGCAATCATGTTCATGTCACTTCCGGTAGTCT TCCAATCGGAAAAGGAAGTTTATTGAACCTCAAGAGTGAAAGACCTCAGAGATTTGTTATCTCAGCTGTTGTGGATGATA AAAGTGTTGTAGCTAAAGAGGAGAAGAAAGATGGTTCTGAAGAGGTTGTTGTAGATAACCAGAGAATGATCAAAGTTTGT GATAAGCTTATTGAGGTTTTTATGGTTGATAAGCCTACACCATCTGATTGGAGAAGATTGTTAGCTTTTAGCAAAGAATG GGATAGTATACGGCCACATTTCTATAAACGGTGTCAGGAACGAGCTGATTCTGAGGATAACCCTGAGATGAAGCATAAGG TTCATCGACTTGCAAGGAAGCTCAAGGAGGTTGATGAAGATATTCAAAGGCATAATGAGCTTCTCAATGTTATCAAGAGG ACACCACCAGCTGAAATTGGTGAACTTGTTGCTAGGCGTCGTAAAGATTTCACAAACGAGTTTTTTGAGCATTTGCATAC TGTTGCTGAATCCTACTATGACAATCCGGATGAGCAAAATGCTCTTGCGTCACTTGGGAAGTTGTCTATAGCTGCTGTAC AAGCTTATGACACTTCAACAGAAAGCATTGATGCGCTAAATGCTGCGGAAATGAAGCTTCAGGACATTATCAACTCTCCT TCGCTTGATGCTGCCTGTAGAAAGATTGATAGTTTGGCTGAGAAGAATCAACTTGATTCTGCGTTGGTTCTTATGATCAC TAAAGCTTGGTCGGCTGCTAAGGAATCCAACATGATGAAAGAAGAGGTAAAAGACATATTGTATCACTTGTACGTAACAG CAAGAGGGAATCTGCAGAGGCTGATGCCGAAAGAGGTGAGAATACTTAAGTATCTACTATCAATAGAGGATCCCCAAGAA CAGATAAGCGCTCTACAAGATGCTTTTACCCCTGGGGACGAGCTCGAAGGGACTGATGTAGACTACTTATACACGACGCC TGAGCATTTGCAGAGCTTGATGAAAACGGTGTTGGAAGCGTATCATTTCAGCAGAGAAGGGAGTCTAGTGAAGGAAGCAA AAGACCTTATGCATCCTGAGCTCATCGCCAAAATCGAACAACTCAAGAAGCTTGTTGAAAAGAAGTATATGTGATAAAAA ACTTTACAGATCCTAAGCTCTCGTAATTTGTTACTATGTATGTTTTGTATATGGTGTATGACATTCTTCAGGTTTCTGTT TCACACATATATG ................................................................................ ............N.....................N............................N................ ................................................................................ N............................N.........................N........................ .......................................................N.....N.................. ..........................................................................N..... ..............................N..................................N.............. .....................i.......................................................i.. ...................................................................i............ .................................N....................N...........N............. ................................................................................ .................N...........N........N......................................... ......N.......................N........N..........N............................. .......N................................................................N....... ...............................N..N............................................. .......................i........................................................ ...................N...................................N........................ ...................i............................................................ ........N...........................................................N........... ...................................N...N..........N......N...................... ..........N.. Pos Score Pred ------------------------ 93 0.292 - 115 0.376 - 144 0.126 - 241 0.275 - 270 0.441 - 296 0.223 - 376 0.269 - 382 0.194 - 475 0.048 - 511 0.111 - 546 0.335 - 582 0.523 Yes 638 0.502 Yes 708 0.831 Yes 754 0.301 - 775 0.080 - 787 0.101 - 898 0.405 - 910 0.058 - 919 0.156 - 967 0.119 - 991 0.287 - 1000 0.188 - 1011 0.487 - 1048 0.100 - 1113 0.451 - 1152 0.285 - 1155 0.234 - 1224 0.710 Yes 1300 0.247 - 1336 0.204 - 1380 0.573 Yes 1449 0.144 - 1509 0.120 - 1556 0.116 - 1560 0.044 - 1571 0.065 - 1578 0.035 - 1611 0.029 -

Explain the output -- Processing locus 431: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATCGATGGGTTCCCGAGCAAAGACGAGCCATATACCGATCACTTCTTCTTTGTAGCCATTGAAGATGCTATTCATGAAG ATCTCCTCGGGACTGTTCTGACGAGATGGGGAATTTTGGGTAGTTGTAGTTAGAAATTTATTAGTACCCGAGGTGATGTT AATTTCGTTCTCTAATTGCAATTTTCCTTTTGCAGAACGCACTCTCAAGTTTCTCGAGCCGATTCCAGACGATTTTCTTT CGGCTTTTCACGCGTTGTCGGCTCGAAAGTGCGATTGGCTGAAGCATTTTTCTCGGGAACGAGTTGAGTGTGCGCTTCGT CTTCTTCACGGCGTTTCTTGTCCCACTAGTTCGGAATCTTCCGATCATCGCACTCAATTTTTCGTGGACATGCAATCGAC GAAACTTACTTTGCGGGAGGTCTATGCCAAGAAGAAAGAGGACAAGGAGAGGCGATTGGCGGAAGAGAGGCGCCTTGTTA ATACGGGATTGATCTCACCGCGGGCCGATCCTGAGGCGACCCAAGATGGGAATGTTATTCCCGATGCTACCGCGCCGCAG ACTGCTCCCGAGGCATCTCGAGATGGGGTCAATCCCTATACTGCCGGGCCGGTTGACGCGGCGCCCGCTGAGGCTCAGGG AGCCGAGCCATCCGCTGCTGCGCCCGAGGCGGTTTTGGCGTTACCTGCGATTGACAAAGCGGCAGGTAAGCGCATTCGGG TTGACGATGTGTCGTCAAAGAAGAAGAAGAAGAAAAAGAAGGCATCCGGCTCCGAGGTGGAGAAAATACTCCCCATCTTT GAAGATCGTACTGCCTCGGCTAATCTGCTCGGGGGATGTGTTGGTCCCTTGCTTCCTCCTCCAGATACTCTTCTGGAATC TCGGAAGTATGCCGAGACGGCATCTCACTTTCTGCGGGCTGTCGCTTCGATGAACCGGATGGTACATTCGTATGATTCGG CTATGCGAAGCAACATGGAGGTGGCTGGTAAATTGGCCGAGGCGGAGTCTCGGATTCAGGCCATCGAGCGAGAGAAGAAC GAGGCACTCTCCGAAGCTGCTGCAGCGAAACTGGAAAAGGAAGAGGTCGAGAGGACGGCTCATGTGAATAAAGAGAACGC CATTAAGATGGCCGAGCAGAATCTCAAGGCGAACTCTGAGATCGTCCGTCTTAAGCGGATGTTATCCGAGGCGAGGGGAC TTCGTGACAGCGAGGTGGCTCGGGCCGTCCAGACGACGAGGCGGGAGGTCTCCGAGACTTTTATTGCTAAAATGAAGAAC GCCGAGCACAAAGTGTCGCTCCTTGATGAGGTCAATGACCGATTCATGTACTTGTCTCAGGCGCGAGCCAACGCGCAGCT GATCGAGGCCCTCGAAGGTGGCGGAGTTCTGGAGAGTGAGAAGGAGCAAGTTGATGAGTGGTTAAAAGATTTCGCCGATG CTGAGGTAAATCTTAATCGCTTCATGTCCGAGCTGAAAGATGACCTCAAAGCTCCAGCTCCCGAGCCCGCTCCTCTGAGT CCGGGTGGTCACAGATCGGTCGAGTCATTGGCTGACGAGGCGGGAATTACAGATCAAGCCGGATCTCTCCTCCCTGCCAA GGATAATCGACCATCCGAAGATCTCGACTAGGGACCCGAGGCGGTTTTTTGTTTTTCTTCTCTCTGCTCGGTTATAATAC TTATAGCTGGATGGCTTTATCCGTTATGTAGAACCTGCCTGGATGGCTTT .....N...........................................................N........N..... .........................N.................................................N.... ................................................................................ ................................................................................ .....................................................................i.......... .......................N........................................................ .............................................i.....N...........N................ ......................N......................................................... ................................................................................ ......N......................................................................... ...................................N............................................ ........N........................................i........i............N........ ..i...........i................................................................. .............................................................N.................. .......i..................................................N..................... .......................................................................i........ .........................N........N..........i.................................. .....................................................N.......................N.. .......................i...............i........................................ ................................................................................ ................................................................................ ..........N..............N................N....... Pos Score Pred ------------------------ 6 0.350 - 66 0.281 - 75 0.111 - 106 0.372 - 156 0.393 - 390 0.602 Yes 424 0.362 - 526 0.608 Yes 532 0.111 - 544 0.289 - 583 0.277 - 727 0.117 - 836 0.484 - 889 0.404 - 930 0.639 Yes 939 0.804 Yes 952 0.265 - 963 0.795 Yes 975 0.877 Yes 1102 0.213 - 1128 0.866 Yes 1179 0.165 - 1272 0.716 Yes 1306 0.341 - 1315 0.361 - 1326 0.662 Yes 1414 0.256 - 1438 0.302 - 1464 0.754 Yes 1480 0.509 Yes 1691 0.045 - 1706 0.061 - 1723 0.108 -

Explain the output -- Processing locus 432: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTATTTTCTACTCGATTTTGCAAATTTGTTGTAATTCTAATTTAAATTTGTTATATGGGCTTGAAAGGCCCATTATACAA TTAGAAGCCCAAGTGTTCTAGGTTAGATTTCTCACTCCCGGAAAAAAGAAGCTGATATATAAATGGCCATTGTTACTTCA GCTTCTACTGTGCGGCGTCTTGATGGAGGAGATAAACCCTAGTTCTTCTGTAGACAATAAGAGAGACATGGCGGAAGAAG CTAAAGTTGATGTGAAGACTTCGGCGAAGAAGGATATACGCAATTATCTATGCCAGTATTGCGGAATCAGCAGATCTAAA AACTATCTCATCACTAAACACATCCAATCTCATCATCAGATGGAACTTGAAGAGGAAAGAGATGATGAAGCTTGTGAGGT TGATGAGGAGTCTTCAAGTAATCATACTTGTCAAGAATGTGGTGCTGAGTTTAAGAAACCTGCTCACTTGAAGCAGCATA TGCAGAGTCATTCGCTCGAGAGATCTTTTACTTGCTATGTGGATGATTGTGCTGCTAGCTATAGGAGGAAGGATCATCTC AATAGGCATCTTCTTACACATAAAGGGAAGCTCTTTAAGTGTCCGAAGGAGAACTGCAAGAGTGAATTCTCAGTACAGGG AAATGTTGGTAGGCATGTTAAGAAATATCATAGTAATGACAACCGTGATAAGGACAATACTGGTTTGGGCGATGGTGATA AGGACAATACTTGTAAGGGGGATGATGATAAGGAAAAATCTGGTAGTGGCGGTTGTGAGAAGGAAAATGAAGGGAATGGC GGAAGTGGTAAGGACAATAATGGTAATGGCGATTCTCAGCCTGCGGAGTGTTCAACTGGTCAGAAGCAGGTTGTCTGCAA AGAAATTGGTTGTGGAAAAGCCTTTAAGTATCCTTCACAGCTTCAAAAGCATCAGGATTCTCATGTGAAATTAGACTCTG TGGAGGCATTTTGTTCCGAGCCTGGGTGTATGAAGTACTTTACCAACGAAGAATGCCTCAAGTCACACATAAGATCCTGT CATCAGCACATCAACTGTGAGATATGTGGTTCTAAGCATTTGAAAAAGAACATCAAGAGACATCTACGGACTCATGATGA AGATTCCTCACCAGGAGAAATCAAGTGTGAAGTTGAGGGTTGCTCTTCGACTTTCTCCAAGGCTTCTAATCTTCAGAAAC ACATGAAAGCAGTGCACGATGATATACGTCCCTTTGTCTGTGGCTTTCCCGGTTGTGGCATGAGATTTGCTTACAAACAT GTCAGAAACAAGCACGAGAATTCCGGGTATCACGTATATACCTGCGGTGATTTTGTCGAAACTGATGAAGATTTCACTTC AAGACCGAGAGGTGGACTAAAGAGGAAACAAGTTACTGCGGAAATGCTGGTACGAAAGAGAGTCATGCCTCCTCGGTTTG ATGCAGAAGAACACGAAACTTGCTAGTAGTGTCCAAGCCTTAATTATATTTTCTGTCTTAAGATAAGTGAAGTAGTTTTG TGTAAAGTTCTTTTTGTTTGTGTGTTGGTAGGAAGAAATATAGAA ......................................................N......................... ..............................................................i................. ......................i............................................i............ .........N.......................................N.............................. .......................................i.....................i..i............... ..N.................................N..........................................i ....................................N.....N..................................... ................................................................................ ..N...........N....................N...................................N........ .....................N..N.........................................N........N.... ...................N.....N...................................................... ..............................................................N................. .............................N......................i........................... .......................N.................................................N..N... ................................................................................ ..i...............N........................................i..................i. ................................................................N............... ...........................................i....................N............... N............................................................................... ............................................. Pos Score Pred ------------------------ 55 0.350 - 143 0.784 Yes 183 0.669 Yes 228 0.591 Yes 250 0.158 - 290 0.171 - 360 0.680 Yes 382 0.563 Yes 385 0.609 Yes 403 0.115 - 437 0.425 - 480 0.547 Yes 517 0.316 - 523 0.344 - 643 0.438 - 655 0.263 - 676 0.321 - 712 0.435 - 742 0.124 - 745 0.279 - 787 0.278 - 796 0.449 - 820 0.289 - 826 0.143 - 943 0.260 - 990 0.467 - 1013 0.700 Yes 1064 0.214 - 1114 0.241 - 1117 0.239 - 1203 0.542 Yes 1219 0.338 - 1260 0.567 Yes 1279 0.588 Yes 1345 0.139 - 1404 0.609 Yes 1425 0.205 - 1441 0.199 -

Explain the output -- Processing locus 433: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTGGGAACCTGAAAATCTCTTCCTCAACTTCATGATCAATTCTTCAGGTATATAAACCCTTTGATGATAAGTTTTTTT CTTGTGGTGTACGTTATCTAACCTAATGATATGTTTTCTTCCTTCAGAATGGGTCTCAAGCTCTCACGAGGGCCTGTAAA AGAAAAATCACCTTTGGAATTCACAAGGGTTCACATTCTGACTTACTTCACCACAAATTCTTACCTACGTAACTTGGTGT CCAAGAAACGAAGACGGTTAATCATCGGTGGATATGATCTCGACATGTCTTACATCTCCGACAAATTATTGGCAATGTCT TTTCCAGCTGAACGAATGAGAGCAGTCTATAGAAACCCTCTTTGGCAAGTCAAGTCCGTGCTCGATATGCGACATCCTGA TCATTACAAGGTCTATAATCTATGTATAGAGGAATCTTATGATCCAGACAACTTTTATGGCCGCGTGGAGCGATTTCCGT TTGATGACAATCATGTTCCATCCCTCAAAATGATCCAACTTTTCTGTGAAAGTGTTCATTCTTGGCTCTCATTAGATCCC AAGAACATCGCGGTTGTTCATTGCATGGCAGGAAAAGGTAGAACAGGGCTAATGGTATCGGCGTACCTTGTTTACGGTGG CATGTCAGCTGAAGAAGCTCTTGAGATGTATGCAAGCAGAAGAACAACAAACAATAATGGAGTCTCAATACCAAGCCAAC GTCGTTATGTGAAGTACTGGTCAGATTTACTTTCGTTCAGTAAAAAAGGACCTCCCGAGGTTAAATTACCGCAAGAACAT AGCAGAGAGTTGTTGCGAATTAGACTTTATGATACAGCTAACGTTGATTCCGTCTTCTTTGTGGTTTCAGAACTTCAGGA GGTTCCAAATGAGATGTATCGACCGTCTGTAGAACTTGCAAGAGGTTGTTGTAGACAATTCAAGAAAGGGTATTGTAGAA GTTCGAGTCCACGGTATTATATATCTCATGTGAACTGTGATTCAGAAGAAGATGAGGAAGTAACAGACGGAGAAGAGCCA CATCTTGTTGTTCAGATGGATACAGAGAGTTCCATTATCGACGAAAAAACTTGTCTTGATTTCTACTTTGACAAACCTGT GAGAGTTAGTGGTGACATACGCATCACATTCTACCAGAAAATGATTGGAAGCCGCCTTTTTTATACTTGCTTCAACACAG CTTTTATAACCAATGGCTTACTTCAGTTTTCCATAGGAGAGCTAGATAAAGTTGGTGGTAACGGAAGATCGATATCTGGT CCTGATTTTAGCTTGGAGTTACTCTTTGGCCCAGCTTGTTCGAAATTCGGAAAGTTTCTTTCCCGCGATGACCTTTCTCT CTCTTGAGGCCTCATTTTCAGCTCATTGGATTTCAACACTTGAGTTTTTAAACGGTTCTGTTTCATCACTCAGCCACAAA AGAGGCCTGACTAGCTCAGCTGAGAC .................................N...............................N.............. .........................N....i.................i............................... ................................................................................ .................................N..........N.............................N..... ...............i..................................................i............. .....................N................N.................N....................... ...N........N................i.................................................. ........................i..........................i............................ ......N......................................................................... ............................N................................................... ........i....N.................................................................. ...........................N.......................N............................ ...............i................................................................ ........................................i....................................... ............i................................................................... ...................................................................N............ ................................................................................ .......................... Pos Score Pred ------------------------ 34 0.079 - 66 0.366 - 106 0.144 - 111 0.548 Yes 129 0.574 Yes 274 0.305 - 285 0.431 - 315 0.490 - 336 0.554 Yes 387 0.555 Yes 422 0.173 - 439 0.183 - 457 0.404 - 484 0.085 - 493 0.364 - 510 0.576 Yes 585 0.753 Yes 612 0.719 Yes 642 0.467 - 666 0.603 Yes 670 0.127 - 697 0.653 Yes 727 0.448 - 829 0.173 - 889 0.544 Yes 894 0.397 - 988 0.242 - 1012 0.132 - 1056 0.717 Yes 1161 0.727 Yes 1213 0.781 Yes 1348 0.358 -

Explain the output -- Processing locus 434: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGTCTGGTATAGAAATCTGAAATGGGTCTATCAAAGTTCGAACACATCATCCATGTTTCTTGATTTTCCCATAGGTGGT GGATTTGGTGAATAGATTTGACTCAGAGCTATAGAAACTAATCTATTTATCAAAATGCCGTGTTTAGATACGCACTATTG GAATTTCACTGGTCTCATAGGCGCATTTCTGAATCTCTCCATAGCTTATTTGCTTCTATGTACATCTCTTTGTATACATC TCCTGTTGAGATTTCTAGGGTTTCTAGGTTTACCTCTTCCAAAATTTCACAATGGACGTTTCGGTGTATCAAACACTATG AGGAAATGCCTCCATGAAGACCTTCTGGTGGATCGTGCATCAGACAGAATCTCAGCCGTAGATCGATCGATAAAAAGCAA AATCCCTTTTGGTTCGATTACTAGTGACGATGATTCGAACCGGTTTGTTGAGTTAAAGCTGGTGAGAAAGAACAGTGGTG ATATAAGCAATGCTAGAGTTAAAGATGAAACAGAGGATTTGTCAAGAGACGAACCAAATGTGATTAACAATAAGGAACTT GGTAGATTTGATCTTATGACCGTTCAGGGCATGAAAGGCAAACGTTTTGTTACAAGAAGATCAAGAAACGGTCTTAAAAA CCATAGGAGATCAGGTCGTGTAAACTGTGCAAGTCTCAGATCTATTTCTTCATCAGAAGCCTTTGATGAGACCGCAAAAA CCAATTTGATGCAGCCTTATGATGAGACATGTTCCAATGATGACCAAAAAGATTGTGGTAAGGCAGGAGAGGGGACTAAT CTATCTCATTGGCCGGAATTTGAGAAAACCGTATCAGTTAATCAACAGGTAAACTCCAATTCATCAGCAGAACAATCTTT TGTGAGAAATGTAGAGAAAAGATCTGTTAGGGATTTAGAAGAATTATTGAAAGAAGAGAGAGCGGCTCGAGCAACGGTTT GTGTCGAGCTTGACAAAGAGAGAAGCGCTGCAGCATCAGCAGCGGATGAAGCGATGGCGATGATACATAGGCTGCAAGAT GAGAAGGCGGCAATTGAAATGGAAGCGAGGCAGTTCCAGAGACTGGTTGAAGAGAGATCGACTTTTGATGCTGAAGAGAT GGTCATACTCAAAGACATTTTGATACGGCGTGAGAGAGAAAAACATTTCTTGGAGAAAGAAGTTGAAGCTTATAGACAAC TACTCGAAGAAACAGAGGAATTGGAATGTTCTTTAATAAAAGAGAAGAATGTTCCAGAACCGGAACACAAACAAAACAAA GACTGTCAAGAGAGAAGAGCTTTGCTCGTTCAGGAACTTGATGGAACGGTTCTCGATATGCCTTATAGAGAAGAAGGAAA CAGAGATAAAAACAGAGATCTGTATAAATCAGATTCAGAAGTCGCGTACTCACGTGTGCGTGATGTTTATATGGTGAAGG ACGAAACAGAGAACATTAGTAAGAAGAAGAATCTAGAAGAATCCTCTGTTGGCAAACCTAAAGAATCGTTGGATGAAAAC AGTATCATTGTCTCGGGGATTGCTCGAAAGCTACCTCCCTTATGTCGTCCACGCAAGAAGTCGCTGAGCTCTTCGGGTTC AAGGAGAAAATCGATGTCTGCGGTTGATTATGAGAGGTTAAAGATTGAAAATGAAGTGGAATTGTTAAGAGAAAGATTAA AGGCTGTTCAAGAGGAAAGAGAGGAGCTTACGAGACGAGCATCTTTGCCTCCTTTACCATCAAAGGTTAGGGCTACGTCA GAGAAGCGAAGTTGGACAAGATCTTGTTCCATGGATCTTCACTCTTCTTGAAGGCTGAAGCCTTTTAAATTTTATGTACT TAGACACATACATGTTTGTATGTATAGTATACAGGATTCGATTATCAGTTACGTGTTTACAGATTTTGAG i.....................N..............................N.......................... ......................................................N......................... .........................................................N...................... ...................................................i.........................N.. .....i.......N.................................................................. .............................N.................................................. .........N..............N................................N...................... ...............N..............N................................................. .................................................................N.............. ........i.........N..N......N.......i..N........................................ ................................................................................ ........N....................................................................... .............................................N.......N.....N..................N. ..................i................................................N..........i. ................................................................................ .........................N......................N............................... ........................................N................N...................... ..............................................................N.......N......... ........................................................................N....... .........................................N...................................... .............i...............N....................N............................. ................................................................................ ..............................N..........................................N...... ...........N.......N.................................................. Pos Score Pred ------------------------ 1 0.524 Yes 23 0.365 - 54 0.304 - 135 0.448 - 218 0.235 - 292 0.749 Yes 318 0.366 - 326 0.523 Yes 334 0.331 - 430 0.274 - 490 0.265 - 505 0.286 - 538 0.266 - 576 0.415 - 591 0.165 - 706 0.209 - 729 0.755 Yes 739 0.364 - 742 0.300 - 749 0.372 - 757 0.686 Yes 760 0.247 - 889 0.396 - 1006 0.059 - 1014 0.496 - 1020 0.275 - 1039 0.144 - 1059 0.585 Yes 1108 0.120 - 1119 0.544 Yes 1226 0.461 - 1249 0.318 - 1321 0.199 - 1338 0.319 - 1423 0.097 - 1431 0.448 - 1513 0.270 - 1562 0.440 - 1614 0.603 Yes 1630 0.235 - 1651 0.410 - 1791 0.168 - 1834 0.048 - 1852 0.058 - 1860 0.060 -

Explain the output -- Processing locus 435: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAACTTTATCTAAATTTCACGCGTTCCGTGTACATATTTTCAATTAAACGTAGGCAATATGGTGACAAAAATGAATAGT TTTCAAATACGTAGGCTCTAAGACATCTGTAATGTATATATAACACCACACACACAAATGTCCTTAACGTTTCATGTTGT GTCATTCTCTTTTCTTCAAGTCGGCAAAAGTATCACAATGTTAAACATCTTACCGTTTTTCTTGTTCTTTCTTCCGTTTC TCATCGGAAACAACCGCATTTGCGTCGCCGTAAAGACAGGCTTTGTCGGGAGAAACGGCACGCAGTTCGTGTTGAACGGA GAACAAGTTTACTTAAACGGCTTCAATGCTTATTGGATGATGACCACCGCGGCTGACACGGCATCTAAAGGAAGAGCTAC CGTCACGACGGCGCTCCGGCAAGCCTCCGCGGTGGGAATGAACGTCGCTAGGATTTGGGGTTTCAACGAAGGAGATTACA TTCCTCTTCAGATCTCTCCTGGCTCCTATAGTGAAGACGTTTTCAAGGGGCTAGACTTTGTCGTCTATGAAGCTGGACGA TTTAATATTAAATTGATAATTAGTTTAGTCAATAATTTCGAAGACTATGGAGGGAGAAAAAAGTACGTGGAATGGGCCGG TTTAGATGAACCGGATGAATTCTACACAAACTCAGCTGTCAAGCAATTCTACAAGAATCACGTGAAGACCGTGTTGACGA GGAAAAACACAATCACTGGTCGAATGTACAAAGATGACCCTACCATCTTCTCATGGGAACTCATCAACGAACCTCGCTGC AATGATTCCACTGCTTCGAATATTCTTCAGGATTGGGTGAAGGAAATGGCTAGTTATGTTAAGTCCATAGACTCAAACCA TTTGCTGGAAATAGGGCTTGAAGGGTTCTACGGAGAATCTATACCGGAAAGAACGGTTTACAATCCCGGCGGAAGGGTCT TAACCGGTACAGATTTCATCACCAATAACCAAATTCCGGATATCGATTTCGCCACTATCCATATCTACCCGGATTCTTGG CTACCATTACAGAGCTCAAGAACAGGAGAACAAGATACATTTGTGGACAGATGGATCGGAGCACATATTGAAGACTGTGA CAATATCATCAAGAAGCCTTTGCTGATAACAGAGTTTGGGAAATCTTCAAAGTACCCTGGTTTTAGCTTAGAGAAACGGA ACAAGTTTTTCCAAAGAGTTTACGATGTTATCTACGACAGTGCAAGGGCTGGTGGTTCGTGTACTGGTGGTGTTTTCTGG CAGCTTACGACCAATAGAACTGGTCTGTTAGGTGATGGGTACGAGGTCTTTATGCAAGCAGGTCCTAATACTACTGCTCA GCTTATCGCCGACCAATCTAGTAAACTGAAAAACCTCAAGTATCCACCGTTGGTCACACATAGTGCAGAATGAAACAGTA TAGATACAAACAGTAGATATTAGAATGTAAAAATAGGAAACATGTATAGTTGAATATGAACAAAAGCTAAATGATATGTA GAATAGTTGAAT ...........................................................i...........N........ ...............................N.........................N...............N...... .....................................N.......................................... ................................................................................ .........................N..........i..i........................................ .....................................N.......................................... ..................................................................N............. ..............................................i........................i........ .....N........N................................................................. .......................i.........N..................N........................... ................................................................................ ................................................................................ ..................................................N............................. ................................................................................ ........................N....................................................... ..................................N................N............................ .....................................................................N.......... ........................N................N.............N..............N....N.... ............ Pos Score Pred ------------------------ 60 0.748 Yes 72 0.171 - 112 0.376 - 138 0.196 - 154 0.350 - 198 0.475 - 346 0.449 - 357 0.585 Yes 360 0.711 Yes 438 0.471 - 547 0.167 - 607 0.609 Yes 632 0.742 Yes 646 0.254 - 655 0.064 - 744 0.680 Yes 754 0.379 - 773 0.354 - 802 0.291 - 846 0.783 Yes 856 0.493 - 1091 0.277 - 1225 0.327 - 1315 0.248 - 1332 0.442 - 1430 0.411 - 1465 0.067 - 1482 0.054 - 1496 0.062 - 1511 0.033 - 1516 0.044 -

Explain the output -- Processing locus 436: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCCAAGTCCAATGTTGTCGTGGAACGAACGTCGTTTAGAATAATTAAGCCCAGTATTTAACATATCAAAGCCCAATGTTG TCCTAAAACGACGACGTTTGAATGTGGAACGTAAACAATCCTGGAACAACCGTTTCAACTACCTTTTTCCTATCACTTGT TTTTTCACCTCTCGAAGTCCTCCGATTTGCTACCCTTACGCAGCCTCATGTGTCAACCACCATTTCTCTTCTTCATCTTC GTCTCTATCAAACCCTAAACCTAAAACCCCTTATTTTCCCGCCAATGTATCAATCCATGAATCTATCAGTTTCTTCCAAT TTCACTCACCGATCACTCCTCGAATCACGATTTCCTATATTCTCCACCGGATTCCGCAAATCCGTCAATCTCAAACCTCC CCGTGTCTCATCTGGTCCTGAATCCAACGATTCAGGTCACGAAACCTTAACCGATAAGCTTATCCATCTCCTCAGAGCTG TTCCAGATTGGGCAGACGAGATTAAAGAGCGTGGGATGCAGCAGAAACGCTCTCTCTACACACACGAGAAATGGGTTGAA CATAGAAGCTCTCTTCGCCATGTGCGTCATCTACTCTCGAGCTTTTCTTCACGAGTTATACTCTCCCTGATTCCACCTGT TTTCTTCTTCACAAGCGTCGCGGTTGTGATCGCTAGCTACAACTCCGCCGTGGCTTTGGATTGGCTTCCTGGTATTTTTC CAATTCTTCGATCCTCGTCGTTGCCGTATCAGCTCACAGCTCCGGCTTTGGCTCTTCTTCTGGTCTTTCGTACGGAGGCT TCGTATTCTAGGTATGAAGAAGGAAGGAAAGCTTGGGTTGGGATTATTGCTGGAACTAATGATTTAGCTAGGCAAGTTAT TTGTTCCGTTGATAGCTCCGGTGATGAATTGATCATCAAAGATTTGCTTCTTCGTTACATTGCAGCTTTCCCTGTTGCTC TTAAGTGTCATGTGATTTATGGTTCAGATATAGCAAGAGATCTCCGGAACTTGATAGAAGCAGATGACTTATCTCTGATT CTTCAAGCAAAGCATCGTCCGCGTTGTGTAATCGAGTTCATTTCTCAGAGCATTCAGTTGCTAAAACTAGACGATGCAAA GAGAGATTTGTTGGAATCAAAGATGCTGCATTTACATGAAGGAATTGGAGTTTGTGAACAACTCATGGGCATTCCGATTC CTCTCTCTTACACACGCTTAACCTCGAGGTTTTTAGTCTTTTGGCATCTAACTCTCCCAATCATTCTCTGGGATGAATGC CATTGGATTGTTGTTCCTGCTACTTTCATTAGTGCTGCCTCTCTGTTCTGCATAGAAGAAGTGGGTGTTCTTATAGAAGA ACCATTTCCTATGTTGGCCTTAGATGAGCTTTGCGATCTTGTGCATAGTAACATCCAAGAAGCTGTTAAATCTGAGAAAG TCATACGTAACCGCATCATTGCAAAGATAAAGCTCCATGAATTCAAGCACTCATCAAATGGTCGCCATAGATCTTGATTA CCGGTTGGTGTGAGTGATGTAGGTTGTCTTCCCGAGGGAGCTAAGAACATGTGATGGCTTAGGTTAGCTTTGATATATAC GTATCGTATGTTAGAG ..........N...............................................................i..... .....................i.......................................................... ...............................................N................................ ............................................N...........N....................... ................................................................................ ................................................................................ ...................................N..................................i......... ...................N............................................................ ................................................................................ ................................................................................ .............N............................................N..................... .......................N........................................................ .........N........N............................................N................ .........................................................................N...... ......................N............N............................N............... ........................................................................N...N... ................................................................................ ..........i............N........................................................ ....................................N....................N...................... ................N...............................N....N.......................... .......N........ Pos Score Pred ------------------------ 11 0.222 - 75 0.551 Yes 102 0.742 Yes 208 0.315 - 285 0.150 - 297 0.385 - 516 0.376 - 551 0.795 Yes 580 0.314 - 814 0.187 - 859 0.214 - 904 0.153 - 970 0.124 - 979 0.115 - 1024 0.433 - 1114 0.330 - 1143 0.352 - 1156 0.293 - 1185 0.308 - 1273 0.250 - 1277 0.446 - 1371 0.807 Yes 1384 0.300 - 1477 0.283 - 1498 0.355 - 1537 0.064 - 1569 0.060 - 1574 0.062 - 1608 0.047 -

Explain the output -- Processing locus 437: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACGATTCTCCGATTATCCCCAAGAGGAGAATCCTCATAATCATTTTCTCCGATTCGATTCGTCTTCCTTGGTCCTGGATT GCTTCATGAATTTCTAGGTGAGAATTTTCGATCCTTCTCCTTTGCCTTTTGGTAATCCACAGAGAGATAGAGACAGAGAA TTTATATCTTTCGAGCATAGTTCGGATTCGATTTGGTTTTGTTTTTCCAGCGAAGTTGATGTTTGGTTGGATAATGATTC TCTCTTGCAGGACAACAATGGAGGCTCGTCCAGTTCATAGATCAGGTTCGAGAGACCTCACACGCACTTCTTCAATCCCA TCTACACAAAAACCTTCACCAGTAGAAGATAGTTTCATGAGATCAGATAACAACAGTCAGTTAATGTCTAGACCATTAGG ACAAACCTACCATTTACTTTCATCTAGTAACGGTGGAGCTGTTGGACATATATGTTCTTCTTCATCATCTGGTTTTGCAA CCAATCTCCATTACTCAACTATGGTATCTCATGAGAAACAACAACACTACACAGGAAGCAGCAGTAATAATGCTGTGCAG ACACCAAGCAACAACGATAGTGCTTGGTGTCATGATTCATTGCCAGGAGGGTTTCTTGACTTCCATGAAACCAACCCGGC GATTCAAAACAACTGTCAGATTGAGGATGGTGGCATTGCGGCTGCTTTTGATGACATTCAAAAACGAAGTGATTGGCATG AATGGGCTGACCATTTGATCACTGATGATGATCCTTTGATGTCTACTAACTGGAATGATCTCTTGCTTGAAACAAATTCC AATTCAGATTCAAAGGACCAGAAGACACTGCAAATTCCGCAACCTCAGATTGTTCAGCAGCAACCTTCTCCGTCTGTGGA ATTGCGACCTGTTAGCACAACATCTTCAAACAGCAATAACGGAACGGGCAAGGCACGAATGCGTTGGACGCCAGAGCTTC ACGAGGCTTTTGTTGAGGCTGTCAACAGTCTTGGCGGTAGTGAAAGAGCTACTCCTAAAGGGGTACTGAAGATTATGAAA GTTGAAGGCTTGACTATATATCATGTTAAAAGCCATTTACAGAAATATAGGACAGCTAGATATCGGCCAGAACCATCAGA AACTGGTTCGCCAGAAAGGAAGTTGACACCGCTTGAACATATAACATCTCTTGATTTGAAAGGTGGGATAGGTATTACAG AGGCTCTACGACTTCAGATGGAAGTACAGAAGCAACTCCATGAGCAGCTCGAGATTCAAAGAAACCTGCAACTCCGAATA GAAGAACAAGGCAAGTACCTGCAAATGATGTTCGAGAAGCAAAACTCTGGTCTTACCAAAGGGACAGCCTCAACATCAGA TTCCGCAGCCAAATCTGAACAAGAAGACAAGAAGACTGCTGATTCGAAGGAGGTTCCAGAAGAAGAAACCAGGAAATGTG AGGAACTAGAATCTCCACAGCCAAAGCGTCCCAAAATCGATAATTGAAAGTATTGGTCTTTTGCTGGATAATCTCGGAGT TTCAGAGTTAACAGTGATAGAGAGAACGAGCTCTTATCTTGAGGTTCTTCAGGACTTCTCTCTTTA ................................................................................ .....N.......................................................................... ..........................................................N..............N...... .................N.............................................................. ....................................N..........................i................ ...................................................N............................ ....................i.........N......................................i.......... ...............................N................................N............... ..........................N.......................N..........................N.. ................................................................................ ..........................................................i..................... ..........................................................................N..... ......................N......................................................... ................................................................................ .................i.....................N........................................ ........................i..i.................................................... ...........................................................................N.... ................................................................................ .................................................................. Pos Score Pred ------------------------ 86 0.373 - 219 0.486 - 234 0.233 - 258 0.444 - 357 0.407 - 384 0.552 Yes 452 0.245 - 501 0.607 Yes 511 0.293 - 550 0.612 Yes 592 0.102 - 625 0.192 - 667 0.409 - 691 0.195 - 718 0.178 - 722 0.519 Yes 745 0.174 - 748 0.245 - 759 0.668 Yes 775 0.137 - 939 0.758 Yes 1035 0.366 - 1063 0.196 - 1218 0.577 Yes 1240 0.342 - 1305 0.773 Yes 1308 0.878 Yes 1436 0.337 -

Explain the output -- Processing locus 438: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATAACAAAATGTTGTTAATTACTATAAGCGTTTAAATGCATACAACTTGATGTATAAGAAAATGTGTATTATCTAGGG ATTTACTTCTCTAACATATAGTTTATGTTTTTAATTTTGATGGTACGTGAAGCAGGTAGTCACTTGAAAACCCATGATTT TCTTCAACCGTTAGAATGTGTTGGTGCTAAAGAAGACGTAAGTAGGATTAACTCTACCACTACAGCGTCCGAGAAGCCAC CGCCCCCTGCTCCACCTCCACCGTTGCAGCACGTGCTTCCCGGTGGTATAGGGACTTATACGATTAGCCCCATTCCTTAC TTCCATCATCATCATCAGAGAATTCCTAAGCCGGAGTTGTCACCACCAATGATGTTCAATGCTAATGAGAGAAACGTTTT GGATGAGAATTCTAACTCTAATTGCAGCTCGTACGCCGCTGCATCAAGCGGGTTCACTCTGTGGGATGAATCTGCTTCTG GGAAGAAGGGACAGACAAGGAAGGAGAATAGTGTTGGGGAGAGAGTAAACATGAGAGCTGATGTTGCAGCAACTGTGGGA CAATGGCCAGTGGCGGAGAGACGGTCCCAGTCTTTGACAAACAACCATATGAGCGGTTTCAGTTCTCTCTCTTCCTCTCA AGGGTCTGTGCTTAAGAGCCAGAGCTTCATGGACATGATAAGGTCAGCAAAAGGAAGTTCACAGGAAGATGATTTAGATG ATGAAGAAGATTTTATCATGAAGAAAGAAAGCTCCTCGACTAGCCAGAGCCATAGAGTGGATTTGAGGGTAAAAGCAGAT GTGAGAGGCTCTCCTAACGATCAAAAGCTGAACACACCTAGGTCTAAACATTCTGCTACAGAGCAACGGAGAAGGAGCAA GATCAATGATAGATTTCAGATGTTGAGACAACTAATACCTAACAGCGACCAAAAGCGGGATAAGGCCTCCTTCTTACTAG AGGTTATCGAGTATATTCAATTCTTACAAGAGAAAGCAGACAAGTATGTGACCTCTTACCAAGGATGGAATCACGAACCT GCGAAGCTATTGAATTGGCAGAGTAACAACAACCAACAGCTAGTACCTGAAGGAGTTGCTTTTGCTCCAAAATTGGAAGA AGAGAAAAACAACATTCCGGTTTCGGTCCTTGCAACTGCACAAGGTGTTGTTATTGATCATCCAACAACCGCGACGACCT CTCCATTTCCATTGTCGATTCAAAGCAACAGTTTTTTCTCTCCTGTGATTGCGGGTAATCCCGTACCCCAGTTCCACGCA AGAGTCGCATCATCAGAGGCTGTAGAGCCAAGTCCGAGTTCCCGGAGTCAAAAAGAAGAAGAAGATGAGGAAGTTCTTGA GGGTAACATCAGAATTTCAAGTGTTTACTCACAAGGATTAGTGAAAACACTGAGAGAAGCATTGGAGAATTCAGGAGTGG ACTTAACGAAAGCAAGCATCTCCGTTGAAATCGAGCTCGCTAAACAATCCTCGTCTTCTTCCTTCAAGGATCATGAAGTT CGTGAACCGGTTTCTCGAACCAGAAACGACAATGTCAAGCAAACTCGGAAACCAAAACGGCTCAAGACGGGACAGTAGAA GTTATAAAAACAAAACCCAAACACACAATCCGGTAAAAGTTAAACACCAAAATTGATTCCTCTGTTTTTTTCTTTAAATC ATTTTATCTCAAAAAGG ..........N..........................N.............N...........N................ ........................N..............i.................................N...... ...............i................................................................ ................................................................................ ................................................N..i......N.....i............... ..i..............................................................N.............. ..................................................N.........N................... ..i.............................................i............................... ............................N.....i.................................N........N.. N................i............................................................N. ................................................................................ .....N.............N............................................................ .............................................N..................i............... ................................................................................ ................................................................................ ................................................................................ ................................................................N............... ................................................................................ ........................................................................i....... ...............................i................................................ ................................................................................ ................. Pos Score Pred ------------------------ 11 0.332 - 38 0.377 - 52 0.456 - 64 0.431 - 105 0.415 - 120 0.563 Yes 154 0.252 - 176 0.787 Yes 369 0.484 - 372 0.686 Yes 379 0.401 - 385 0.552 Yes 403 0.512 Yes 466 0.130 - 531 0.333 - 541 0.375 - 563 0.519 Yes 609 0.687 Yes 669 0.311 - 675 0.538 Yes 709 0.344 - 718 0.269 - 721 0.164 - 738 0.671 Yes 799 0.360 - 886 0.475 - 900 0.314 - 1006 0.303 - 1025 0.541 Yes 1345 0.342 - 1513 0.597 Yes 1552 0.511 Yes

Explain the output -- Processing locus 439: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAGTATTTGATCTAATTATTTTTGATCTAATTATAAAAAAATCTTAATAAACCGCCTAAAATCATAATCATATCAAATG TTTTTTTTTAATAAAAAATAATCTAAATTTTTATGCCAAAAAGAAGAATTTAGCGTAATGGTATCTATTGGAAAGACGAC CCAAATCCAAAGACCAAACCAGGTTACTTCCACAATGTTCACGGAGTCATCTCCAGCAATGGATCCGGATTTACCCGAAT CCGAAACCCGACCCGAATTCAATCCCTCCTCCACTGATTCCACCGCCATGGCAGAGTCAACCGATGCAGGGGAACCGACG GCATTCGAATTAGCCTCATCCCAAGTCACATCCGTCGCCGATCTACCACCGGAACGATTCAATTCGCTCGACGAATTAAC ACACGATCTCGGCTCGCTCCACGAGCTTTCGACCCGTGGCTCATGGCAAGCGATCCTCGAGAAGATATCTCAAGCCAGAG CTCTCTTCCTCCTCACAAAGCCTCACGAGCATCTCACTTATCTCACTTACCAAGTTATGGCTCTCGCGAAGCTTCGTCGC TCCGATGAAGCTTCTCATGAGCTTAATTCCTTGCACGATTTCGACGGGACGCATTACAGGTACGAATCGTTCCCTGAGAT TTACCCTAATCGGAGAGGATCTATGGTTCCTTTCTCTTTACGATGGCTCTACGCTTTGATCCCGACTAAGCTTGGTAATC GTCAGGAAGGGTTAGATCGTTTATATGTTTTGCTTGACTTTGTTCGAGATCGAATCAGAGAGAAAGAATCTCAGAGTTTA GAGAGTTCTGTGGAATTGTGGAAGAAAAGAGAGACATTTGTGATGAATTGCTTGCTAGGGTTTCATTTAGGTCACAAGGA ATTTGGGGTGTCTTTGGATTTGATGAAGGAATTGATCAATCGTGATCCGTTAGACCCGGTTTTGATTTCGAAACTAGGGT CTGTTCAGATGCAGTTTGGTGATGTAGAAGGAGCTAAAACTACGTTTGATAGAGTTGAGAAGATGTTGAATGAAGGAAAG AGCAATGGTTTATTGAACGAGATACAGTTCAACAATCTTGTTGGTAGGAATAAGGCTTTGGTTTATGTTGTGGCAAAGGA CTATGTTTCTGCTGTGAGAGAGTATGACAAATGCATTGAAAGGGATAACTCTGATATTATCGCTGTCAACAATAAGGCTC TTTGTTTGATGTACTTGAGAGATCTATCGGATGCGATTAAGGTGATGGAAAGTGCATTGGAGAGAGTGCCAACCGCGGCT TTGAACGAGAGCTTGGTGGTGAATTTGTGTAGTATGTATGAGTTGGCTTATGTTAATCATACTGATGTTAAGCGGACGTT AAACAATTGGATTGCACGTGTTGCTCCTGATGACTTTGATTCATCTTGTACCAGAGTTTGAGGTATTGTCTCTGTTCTTG TTTCATTCTTGAATTTTTCCCTTTTTTAGGTTTTGGCATGTCTAAGAACTGATTGGTGATGTTATGATGACTTCGATAAA .............................................................................i.. ................................N........................i...................... ..................................i.......................i..................... ...............................................i...............i................ ................................................................................ ..........................................N..................................... ........................................................i....................... ....N...........N............................................................... ......................N...................N..................................... ........................N....................................................... ..........................................N..................................... ......................N......................................................... ........N............N........................................i......N.......... ....N...........................................................N............... ..N....................N......N................................................. ........N.....................N.............N................................... .................................N...N...........N..............N............... .............................N.................................................. .....................................N....................N....N..N............. Pos Score Pred ------------------------ 78 0.684 Yes 113 0.406 - 138 0.734 Yes 195 0.705 Yes 219 0.857 Yes 288 0.635 Yes 304 0.637 Yes 443 0.316 - 537 0.690 Yes 565 0.415 - 577 0.287 - 663 0.296 - 683 0.374 - 745 0.318 - 843 0.190 - 903 0.401 - 969 0.493 - 982 0.166 - 1023 0.692 Yes 1030 0.299 - 1045 0.319 - 1105 0.135 - 1123 0.252 - 1144 0.119 - 1151 0.401 - 1209 0.195 - 1231 0.069 - 1245 0.413 - 1314 0.492 - 1318 0.122 - 1330 0.120 - 1345 0.146 - 1390 0.150 - 1478 0.047 - 1499 0.043 - 1504 0.055 - 1507 0.074 -

Explain the output -- Processing locus 440: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAAAAAAGAAGAAAAAATAGTTAAAAAGTGATATAATTATTTTGCAGTTAGCACACTTTTGTTTCTGGCATGATTCACA GGTCGAAACGATGTCGTTTAGTGTCTTTCCCAGAAAAAACGGAGATCAAATAGGTCCCAAATGGATCCCACAAACAGCAG ACACGGCGCTTCTTTTGAATTTTCTACTCGGCGTTTTACACTTTCCACGTTTGGTCAAAACGCTCCTTCTCTATACGTAA CGACCGTTTATAACTCCGCTATCATCTACCCAGTCAAACACTCACCACGTGTCTATTTACCGTCTAATCCGACACCGTTA ATTCCGCTCCTAAGAATCGCCTTAAAAACCACGTTTTCATTTTCTCATCTTCAAACCTCTCTGTCATTACTCTGGTCAGC GATTCTTCTTTCTTCTTCTTCTCAGATCCTTTCACCGAGTAAGAAGATTTTAAAGATGCCGTCAACGCCGACGACGCCGA CTACAACAACGCCGATCACGGCTTCGTATTGGTGTTACAGCTGTACGCGTTTCGTCAGCGTTTGGGCTGATCAAGGCACA ACAACCGTCGGTAGCGTCGCTTGCCCTCACTGCGACGGCGGTTTTATCGAACAGATTAACGATTCTTCCTCCGCCGCTAC CGAATTAACGATTCCGGCTTCTACTGAAGTAAGATCGATCAATAACAACCGTAGATCCGTGATTAGACGCCGGAGATCCG GTCGTCGTCCGTCCTTTAATCCGGTCATCGTACTTCAAGGAGGCGCCGGCGAGAGAGAAGAGGGAGAAGAAGGAGATGCG GCTAGAGATCGACGCGCTTTTGAGTTTTATTACGATGACGGATCCGGATCGGGTCTTAGACCGCTTCCGGATTCTGTATC TGAGATCTTGATGGGATCTGGATTCGAGCGGTTACTTGAACAGCTAAGCCAAATCGAAGCGTCGGCTACTGGAATTGGTA GATCTGGAAACCCTCCGGCGTCTAAATCGGCGATTGAGTCTTTACCTAGAGTCGAAATCTCGGATTGCCACATAGGCTCG GAGGCTAATTGCGCTGTTTGCACGGAGATTTTCGAGACGGAGACGGAGGCGCGTGAGATGCCGTGTAAACACCTTTTCCA CGACGATTGCATCGTACCGTGGCTATCGATTCGAAACTCTTGTCCCGTGTGCAGATTCGAGCTACCTTCAGAGCCTAATC GACGGAGCAACAACAACGAGGAGGATAACGCCGTGGGGATGACGATTTGGAGACTTCCCGGTGGTGGATTCGCCGTAGGG AGGTTTAACGCAGCAATGAGAGACGGAGAGAGAGTTTTGCCAGTGGTGTTAACAGAAATGGATGGTGGTGGGATTGGTAA TAGCCAGGATGGTCCTAGACGGATCTCATGGGTTAGGTCACACGGTACACTCGAATCGGATAGTAACGGTACTGGCTCAG GTTCCGGCTCCGGTGGAAGATTGAGGAGGATGGTTCGTGGAATGGTGTCGTTAATGAGGAGAGTGAGACCAAACCGTAGC TCTTCTTCTTCATCAGCTGCGATATCAACTTCTTCTGATAATCTGGATTTGAACATTGAAGTAGAATCTAGGGTTTTGGA TAGGTCAAATTCAGTGATGAGAAGATACTTCAGAAGAAACAGAAGTAACAGGGATTCTTCGTCTTCAGTTCTTCATTGAT TCAGAAATATATATATATTATTTTTTGGTTTGTTCATTCAACAATGTAAATATAAGATTCAGAAGAAAAAGATTGGTGCT TCGTTTTCTATTATCAAT ......................................................................N......... ..........N.................................................i................... ................................................................................ ................................................................................ ................................................................................ .......................................................i........................ ................................................................................ ................................................................................ ................................................................................ ...........................................................................N.... ..................................N............................................. ..........N..................................................................... ................................................................................ .........................................................N...................... ................................................................................ ......................................N......................................... ...............i.........................................i...i.................. ........N..................i.................................................... .............................i...........i...........N.......................... ................................................................................ ................N............................................................... ...........................................N.................................... .................. Pos Score Pred ------------------------ 71 0.405 - 91 0.334 - 141 0.504 Yes 456 0.752 Yes 796 0.428 - 835 0.487 - 891 0.395 - 1098 0.434 - 1239 0.343 - 1296 0.802 Yes 1338 0.591 Yes 1342 0.721 Yes 1369 0.396 - 1388 0.532 Yes 1470 0.559 Yes 1482 0.531 Yes 1494 0.206 - 1617 0.083 - 1724 0.066 -

Explain the output -- Processing locus 441: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGCATCCTTTGGAATAGTCAAGTTCTCCGGGCTTGAAGCCCTCGAAACCCCTTTTATCGCGGGGTAAGATTTCGATTTC TACAGAGGTTCTTGTGTTTAATTATTTGGATGCTTAATGTTGATGTCACTTTTTTTTTTGTGTGCAGGTTCCTCAGTTAA CTTGAGGATTTTATATTTCATCATACATTTGAGGAGCAATTTGTTTTTTTATTTTATTTTTGAGAAGGAGATCATGTTTC GTGATAGTTTTTTGTTTGCCCGTACCATCGGTTGCTTTGGATGCTTTGGCAGTTCTGGTAGTAGAAACCAACAATCGCCT AAGCCCTACGATGATGATACACATTCCTGTGATAGTGATGTAACTAGCACTGCAAGAGGAGAAGAAGAAGAAGATGAAGA AGAAGTAGAGCAGAAGAGCCGCTCTAAACGATCTGAGGAAATTTTGAAATATAGATTAGATAATGGATTAATCTGCAGAC ACATTCCAGTGAGAGAAACTAATGAACTTATACGTGGAGAGGATGAAAATGGTGACAAGACAATCAATGAGTATGTTCGC GTGTGTAAAATTGGATCCGGTAGCTATGGTAAAGTGGTTTTATATCGAAGCACTTTAGACGGCCAGTATTATGCTATCAA GGCATTTCACAAGTCGCATTTATTAAGGCTAAGGGTGGCACCGTCAGAGACTGCTATGAGTGATGTTCTTCGTGAGGTTA TGATTATGAAAATTTTGGAGCATCCTAACATCGTTAACCTCATTGAGGTGATAGATGACCCTGAAACTGATCATTTTTAC ATGGTTCTTGAATACGTTGATGGAAAATGGGTGTATGACGGTTCTGGACCGCCGGGTGCTCTCGGAGAGAAAACTGCTAG AAAGTATTTGCGGGATATTGTTACTGGACTGATGTATCTTCATGCTCATGATGTTATTCACGGGGACATTAAACCCGACA ATTTGCTGGTTACTAGTAGCGGTACAGTGAAGATAGGAGATTTCAGTGTCAGCCAAGTTTTCAAGGATGATGACGATCAG CTGCGCCGATCTCCCGGGACTCCTGTCTTCACTGCTCCAGAATGTTGTTTAGTATCAGGTATAACGTATAGTGGCAGAGC TGCGGATACATGGGCAGTGGGAGTTACATTGTATTGTATGATATTGGGACAATACCCGTTTCTTGCGGATACTCTTCAGG ACACCTACGACAAGATTGTTAACAACCCTCTGATAATCCCGGATGGATTGAATCCTCTTCTCAGAGATTTGATCGAAGGT CTCCTTTGCAAAGATCCGAGCCAGAGAATGACACTGAAGAATGTGTCGGAGCATCCATGGGTCATTGGAGAAGACGGGCA TGTCCCGGAGTATTTTTGTTGGTGCAAACGCAATGCTGCCTCAAAGATTGAAGAAGGAGAAGCTAATGGGATCTCTGAAA CCTCAGATCCTAACTAAAAAAACTTTGATGCTTTTGATCGTTTTAAGAAATGTACAAGCTTTGAAGAATAGATGCATCTA CTTAACCGTACAAGTTTGACCTCGCTTGGGGTCATT ................................................................................ .............................N......N.....N..................................... .........................................................................i...... ........................................N....................................... ..........N..N.......................N...................................N...... ..............................................................N................. .....................N....................N.....i.................N.....N....... .........................N............................................N......... .......................................................i......N................N .....N................................................i......................... N..................N......i.......N............................................. ...............................i.........N.....N..N............................. ..................................................................N..N.......... .........................................N...................................... .........i...........................N.......................................... ..........................................N..................................... ...........................i............i...............N......................N ................................N................................N.............. ...........................N.....................N.....................N........ .................................... Pos Score Pred ------------------------ 110 0.233 - 117 0.101 - 123 0.156 - 234 0.601 Yes 281 0.220 - 331 0.490 - 334 0.387 - 358 0.168 - 394 0.247 - 463 0.376 - 502 0.485 - 523 0.185 - 529 0.638 Yes 547 0.327 - 553 0.248 - 586 0.384 - 631 0.132 - 696 0.732 Yes 703 0.077 - 720 0.289 - 726 0.419 - 775 0.562 Yes 801 0.373 - 820 0.088 - 827 0.632 Yes 835 0.156 - 912 0.613 Yes 922 0.114 - 928 0.161 - 931 0.186 - 1027 0.186 - 1030 0.191 - 1082 0.158 - 1130 0.596 Yes 1158 0.187 - 1243 0.186 - 1308 0.737 Yes 1321 0.827 Yes 1337 0.410 - 1360 0.404 - 1393 0.265 - 1426 0.286 - 1468 0.063 - 1490 0.132 - 1512 0.072 -

Explain the output -- Processing locus 442: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAACAACCATAATTCTTCTTTGAATCATCTTCCTAGAAAATCCATTACTACTATGTAAGTTCTTTTATTCAAACTTATAT TAAAAAAAAAAAAAAGTTTGATTAGTTCAAGATCTGAGTATTTATAGTATTGAAAACTTTAGGGGTGAAGATGATGATCT TATGGAGCTTTTATGGCAGAACGGTCAAGTTGTTGTTCAAAACCAGAGACTTCACACCAAGAAACCTTCTTCTTCTCCAC CGAAGCTTCTTCCTTCTATGGATCCTCAGCAGCAACCTTCTTCAGATCAGAATCTTTTTATTCAAGAAGATGAAATGACT TCTTGGCTTCATTATCCTCTCCGTGACGATGATTTCTGCTCAGATCTTCTCTTCTCCGCCGCACCTACTGCGACGGCTAC CGCGACGGTGAGTCAAGTCACCGCCGCGAGACCGCCAGTATCTTCGACGAATGAGTCGAGGCCGCCGGTGAGGAACTTCA TGAATTTCTCGAGGCTGAGAGGGGATTTTAATAACGGTAGAGGTGGTGAATCTGGACCGTTGCTTTCGAAGGCGGTTGTG AGAGAATCTACGCAGGTAAGTCCTAGCGCAACACCGTCGGCGGCGGCGAGTGAATCCGGTTTAACACGGCGGACGGATGG TACTGACAGTTCCGCCGTAGCTGGAGGCGGCGCGTATAATCGGAAGGGAAAAGCAGTGGCTATGACGGCGCCGGCGATCG AGATAACCGGTACATCGTCATCTGTAGTGTCAAAGAGCGAAATCGAACCGGAGAAGACGAACGTCGATGATAGGAAACGA AAAGAGAGAGAAGCCACCACTACTGATGAAACTGAATCCCGTAGCGAGGAAACAAAACAAGCACGTGTATCAACAACATC TACCAAGAGATCTCGTGCTGCTGAAGTTCATAATCTCTCTGAAAGAAAACGGAGAGATAGGATCAATGAGAGAATGAAAG CTTTGCAAGAACTTATACCTCGCTGCAACAAGTCAGATAAAGCTTCGATGCTAGATGAAGCTATTGAGTACATGAAATCT CTTCAGCTTCAAATACAGATGATGTCAATGGGATGTGGAATGATGCCAATGATGTATCCGGGCATGCAACAGTACATGCC TCATATGGCGATGGGTATGGGTATGAACCAGCCTATTCCTCCTCCTTCCTTCATGCCATTCCCCAACATGTTAGCCGCTC AAAGACCTTTGCCTACACAAACTCACATGGCCGGGTCAGGACCGCAATACCCTGTTCATGCTTCTGACCCGTCAAGAGTC TTTGTACCGAACCAGCAGTATGATCCAACCTCGGGCCAGCCTCAGTATCCAGCTGGTTACACGGATCCATATCAGCAGTT CCGCGGTCTCCACCCGACCCAACCACCTCAGTTTCAGAATCAAGCAACATCGTACCCAAGTTCGAGCAGGGTGAGTAGTA GTAAGGAATCTGAGGATCACGGAAACCACACAACAGGTTAATAATGTCCATGGAGCAACAAGAAGATCTGTTTTCACAAG CAAACACAATTTCTTATCCGACCCGACCCAACCACCTCAGTTTCAGGTACTAAACAGTCA ....................................................N........................... ......................................................................N..N...... .................N...................................................N....i..... ............................i................................................... ..................................................N............................N ................................................................................ ............................................................................N... .............................................................i.................. ..................................................................N............. .........................N...................................................... .................................................................N.......i...... ...............................................N......N................N........ ..................N..i.....N....i......N..N.....N..N...........N...........N.... ....i.....i.....i.....N.............................N..............i............ ..........................i..............................N...................... ...................N............................................................ ................................................................................ ...........................................N.....N.............................. ............................................................ Pos Score Pred ------------------------ 53 0.148 - 151 0.218 - 154 0.116 - 162 0.337 - 173 0.485 - 258 0.370 - 310 0.255 - 315 0.908 Yes 349 0.567 Yes 451 0.306 - 480 0.439 - 637 0.293 - 702 0.532 Yes 787 0.210 - 826 0.381 - 946 0.289 - 954 0.538 Yes 1008 0.245 - 1015 0.135 - 1032 0.345 - 1059 0.272 - 1062 0.609 Yes 1068 0.453 - 1073 0.733 Yes 1080 0.173 - 1083 0.258 - 1089 0.323 - 1092 0.176 - 1104 0.275 - 1116 0.310 - 1125 0.597 Yes 1131 0.530 Yes 1137 0.712 Yes 1143 0.461 - 1173 0.321 - 1188 0.769 Yes 1227 0.674 Yes 1258 0.421 - 1300 0.484 - 1484 0.380 - 1490 0.223 -

Explain the output -- Processing locus 443: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGACCAATACAACAAATCCTAAAAATGGCCAACGATAAGAATCACAACAGAAATTTAAAGACTACATGTAACTATTTAT ACTACAGAAAACACCAAATAGAATCCAATCAAAAAAAAAAAAATGAAGATCTTTTGTTTAATTCTCTTCTTAATCTCCTC CTTCATCTCTACTTCTCTTGCCGTCGAACCTCCGCCTGAAACAATCTACCAAAACTTTCTCCAATGTTTCACAAACCAAA CAAAAGCTCCTCCTAACTCATTAGCCGACGTCGTTTTACCAAAAACCGCTGCCGCTTTCACCCCCGTCTTACGCGCTTAC ATCCGTAACGCGCGTTTCAACACCACAGCGACTCCGAAACCCGCGATCGTAATCGCGGCGCGTTCCGAGAGTCACGTCCA AGCTGCCGTCATCTGCACGAAATCGCTAAACATCCAACTGAAAACTCGAAGCGGTGGTCATGACTACGAAGGTGTTTCCT ACATCTCTCACGTTCCTTTCTTCGTCCTCGACATGTCAAATCTCCGTAACATCACCGTCGACCCCGCCACAGAGTCTGCA TGGGTCGGTGCCGGTGCAACACTCGGTGAAGTTTACTACAGGATTTGGGAAAAAACCAAAAGTCATGGATTCCCCGCCGG AGTTTGTCCCACGGTTGGAGCCGGAGGTCACATTAGCGGTGGTGGCTACGGAAACATGATCCGAAAATACGGACTCTCCG TTGATTACGTCACCGACGCCAAAATCGTTGATGTTAATGGACAGGTTCTTGATCGGAAAGGAATGGGAGAAGACATGTTT TGGGCCATTAACGGTGGAGGCGGTGCTAGCTTCGGTGTTATCCTAGCTTTCAAGATCAAACTCGTCCCTGTTCCACCGAC CGTAACCGTTTTCAGAGTAGAGAAAAACTTAGTCGAAAATGCTACTGAAATGGTTCATAAATGGCAATTTGTTGCACCCA AGACCGATCCAGGTCTATTCATGAGGCTATTGTTGCAGCCGGTGACTAGGAATAAAATGCAAACGGTTCGAGCATCGGTT GTGGCTCTCTTCTTGGGGGACCAAAACACTGTTATGTCTATGCTAACCAAAGAATTCCCGGAGCTTGGTTTGAAGAAGGA GAACTGCACGGAGATGACTTGGATACAGTCGGTGATGTGGTGGGCAAACAACGATAACGCTACGCAGATTAAACCGGAGA TTCTACTGGACCGAAACCCTGATATGGCGACTTTCGGGAAAAGGAAATCTGATTTCGTTGAGAAAGAGATCACTAAAGAT GGTTTAGATTTCTTGTTCAAGAAAATGATTGAAGTTGGGAAGATAGGGTTAGTGTTTAATCCTTACGGAGGGATAATGAG TACGGTGGCTACAACGAAGACACCATTCCCACATAGGAAGAAGCTTTACAAAATCCAGCATTCGATGAACTGGAAAGATC CGGGAACCGAAGCGGAAACCAGTTTCTTGCAGAAGGCGAAAAGCTTTTATAGCTACATGGCTCCTTTTGTGACCAAGAAC CCTAGACACACTTATATCAATTACAGGGATCTGGATATTGGAGTTAATACCCCTGGCCCAAACAGTTACAGAGTAGCAGA GGTTTTCGGGAGGATGTATTTCGGTGAAAATTTTGATCGGTTGGTGAAAGTGAAAACTGCTGTGGATCCGCAAAATTTCT TCAGAGACGAACAGAGTATACCTACCTTGCCTGGCAAGCCAGCTAGGCGTTAGTTTGTGTAGTCACCTTACTTGGTTCCC TTTGATTTCCTTAACCACAATGTAAAATATGTATGCTATCTGAAAAATGATATTTCCTTTTTGTAATTATAC .........................i........................................N............. ..........................................i..................................... ...............................................................i................ ................................................................................ ................................................................................ ...........................................................i.................... ................................i..............................................N ................................................................i............... .......................................................i........................ ..............................N.....N.........................i...........i..... ................................................................................ ......................................N..........i..........i................... ....................N...................................N....................... .................................N.....N........................................ .............N....................N............................................. .......................i......................................................N. ........................N..................................................i.... ................................................................N............... ........................................................i....................... ................................................................................ .............i.................................................................. ................................................................................ ...................N........N...N.............N......................... Pos Score Pred ------------------------ 26 0.790 Yes 67 0.174 - 123 0.676 Yes 224 0.588 Yes 460 0.567 Yes 513 0.659 Yes 560 0.454 - 625 0.728 Yes 696 0.829 Yes 751 0.252 - 757 0.200 - 783 0.694 Yes 795 0.603 Yes 919 0.201 - 930 0.794 Yes 941 0.673 Yes 981 0.427 - 1017 0.488 - 1074 0.346 - 1080 0.260 - 1134 0.288 - 1155 0.440 - 1224 0.619 Yes 1279 0.171 - 1305 0.379 - 1356 0.667 Yes 1425 0.258 - 1497 0.706 Yes 1614 0.685 Yes 1780 0.071 - 1789 0.086 - 1793 0.053 - 1807 0.078 -

Explain the output -- Processing locus 444: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACCGACTAATCTTCATTTTTTCATATAATTAATTGTTGTCATGTGTAACACATGCAAAAATACTCTCATTTTTTAAGAT TCCGCGTAATTCAACTTTTAGGAAAACAAATCTCAACGCGAATCTCAAACATTCAAGAATACCATCACTTTTGTGTTAAA AAATGTACTAATCACCATTTTAATTCTTAGATCCAAAATGTATTTTACATCTATAAATAACATACATCGTAGTCCATCTC CATCACAAACAATCACATTCATCATTTCATTTACCAAAAAAAATTATAACCTGAATATAATAGAGATGGGTAGTAGCCTC ACGCGCCTACTAGTCTTCCTTTCCATTTTCGCAGCCATAGCTCTGAAATCAAACTCTCAATACCTTCTTCCGATCACCAA GCACGAACCCACCAATCTATTCTACACCACTTTCAACGTCGGATCCGCCGCAAAATCTCCTGTAAACCTCCTCCTCGACC TCGGAACAAACCTCACGTGGCTCGACTGCCGCAAACTTAAATCTTTATCATCTCTCCGCCTAGTCACTTGCCAGAGCTCC ACATGCAAGTCTATCCCCGGTAATGGATGCGCTGGCAAATCTTGTCTTTACAAGCAACCAAACCCACTCGGACAAAACCC CGTTGTAACCGGCCGTGTCGTCCAAGACAGAGCTTCCCTCTACACCACAGACGGCGGCAAGTTCCTCTCTCAAGTCTCCG TCCGCCACTTCACATTCTCATGCGCCGGTGAGAAAGCCCTCCAAGGCCTTCCTCCTCCAGTCGACGGAGTTCTGGCTCTT TCTCCCGGATCTTCGTCGTTCACGAAACAAGTGACGTCAGCGTTTAACGTCATCCCTAAATTCTCTCTCTGCTTGCCTTC TTCCGGTACCGGTCATTTCTATATCGCCGGCATTCACTACTTTATCCCACCATTCAATAGTAGCGATAATCCGATTCCAA GGACTTTAACTCCCATTAAAGGCACTGACTCAGGAGATTATCTAATCACTGTAAAATCTATCTACGTTGGTGGAACTGCT TTGAAGTTGAACCCTGATTTGCTAACCGGCGGAGCCAAACTCAGTACGGTGGTTCACTACACCGTACTTCAAACCGATAT CTACAATGCTCTTGCTCAGTCTTTTACACTCAAGGCTAAGGCTATGGGAATAGCTAAGGTTCCATCGGTGGCGCCGTTTA AACATTGTTTTGATTCACGCACCGCTGGAAAGAATTTGACGGCGGGGCCTAACGTGCCCGTGATAGAGATTGGTCTGCCG GGGAGGATAGGGGAGGTAAAGTGGGGGTTCTACGGTGCGAATACGGTGGTGAAAGTGAAGGAAACGGTGATGTGTTTGGC GTTTATCGACGGTGGAAAGACACCGAAGGATTTGATGGTGATTGGGACACATCAGCTTCAAGATCATATGCTCGAGTTCG ATTTCAGCGGGACGGTTCTCGCTTTTAGTGAGTCACTCTTGCTCCATAACACAAGCTGCTCCACTTGGCCTTCCCAAAAA TAGAATCATCAGTCACTCATGATAGTATCACAGTTTTTGGTCTTGTATGACACGTTTTCAGCGACACATGTGTTCTAATC GAAATTTTAAGAAATTATCACC .........................................i..........N........................... ................................................................................ ..N..................................N.......................................... .................................................................i.............. ................................................................................ ................................................................................ ................................................................................ ..i...................i...N..................................................... ................................................................................ ...................N............................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ .....N.....................................i.................................... ................................................................................ .....................................................................N.......... ..................................N................................N............ ................................................................................ ..................N...........................N....................N............ ...................... Pos Score Pred ------------------------ 42 0.548 Yes 53 0.308 - 163 0.304 - 198 0.260 - 306 0.543 Yes 563 0.624 Yes 583 0.670 Yes 587 0.328 - 740 0.413 - 1126 0.363 - 1164 0.672 Yes 1350 0.308 - 1395 0.373 - 1428 0.245 - 1539 0.164 - 1567 0.061 - 1588 0.109 -

Explain the output -- Processing locus 445: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACGCATTATAGAATAGCCCGTTTGCTTGCTAAAGATACCATGGCATGGTGAATATTAACAAATTGCTCAGCTTGGTGTT GTACTTATCATGGACACATAGTTCACTTTTTTTTTAGTCATGTCTCTTCTGGATGTGCAAGTTTCCAACAATAGACTCTA TCTACATGTGCTGATACTAATAGGCCTTCATCTTTTGCAGGATTGTTTGTTAGGTTTTACAATGGACTCCCCACAATCTG TTGTTTCACCATTCAAGATTGGTGAGTCAGAGAATGAGAACTCAAATTCTGTGCAGAGTTCTGGAAACCAATCGAATGGC ATAAATTCCAATGGGAAAGATTCTAAAAGTTGTGGCCGGCAAGACCTTGTTGGTGCTCTTGAGGTTTATGTCCATCAGGC AAGGGATATCCATAACATTTGTATATATCACAAGCAAGACGTTTATGCCAAGCTTTGCCTTACAAGTGATCCTGATAAGT CTGTCTCGACCAAAATCATCAATGGCGGTGGGAGGAATCCGGTTTTTGATGACAATGTTAAGCTTGATGTCAGGGTTCTG GACACTTCCCTCAAATGTGAGATTTACATGATGAGCAGGGTGAAGAATTATCTTGAGGACCAGCTGCTTGGTTTTACTCT GGTTCCTATGTCGGAACTGCTCTTCAAGAATGGGAAACTCGAGAAAGAGTTCTCTCTTTCTTCCACTGATCTCTACCATT CGCCAGCTGGTTTTGTTCAATTGTCACTCTCCTATTATGGATCCTACCCTGACGTGATGGCTATTCCCTCTATGCCTTCG TCTGTTTCTATTGATGAAACTACCAAGGATCCAGAAGGGTCTGAATCAGTTCCGGGTGAGTTGGATAAGATAGAGTTTCC AGATCCTAATGTTGCTAATGAAAATGAGAAGATGGTTTCCGAGTATTTTGGGATCTCTTGTTCCACTATTGACTCAGAAA CTTCTGACAGCTTGGTTACTTCCGATGCTGAGAATCATGTGACTAATTCTGTCACTTCTATACTGAAGCAAGATTCACCT GAGAGCAGCAATGCAACAAATGGAGCTGCTTCTCCTCACGCTTCAGCTCACTCCGCCACAGAAACACCAAACCATGAACA TCTCTCTGTCGTGAACTCCAAAGCGAGTTCCCAGGAGTCAGAGAGCGAGGCTTCTGGTGAGACATCTGAGGAGAAGACTG TGAAATCCGTCCTCACTGTGAAGGTTGAGCCAGAGTCAAAGGTGGTGCAGCAGGATATAGTCGACATGTACATGAAAAGC ATGCAACAGTTTACAGATTCACTGGCTAAGATGAAGCTTCCTCTGGACATTGACAGCCCAACAAAATCAGAAAATTCAAG CTCTGATTCTCAGAAGCTGCCAACACCAAAGAGCAACAACGGATCCCGTGTGTTCTATGGGAGCAGGCCTTTCTTCTGAG CATCTCTTTTACCAAGTTCAGAGAAAGAACGCAGGTCACCCTTAGACTTTTCTAATGAAGAAAGACTAATCCTAATGATG TAATTTTATAATGTACTA ........................................N....N.................................. .........N.............................N............N........................... .....N.......................................................i.................. .................................N.........................................N.... ..........N........................................................N............ ............................................N................................... .....................N..........................N.....N...........N............. ..............N............N..i................................................. .......N.....................i.................................................. ....................................N...................i..............i........ .............N.................................................................. ........N........N.....N.......i................................................ ........................N...........N........................................... ..........N........i.....................................................N...... ................................................................................ .................................................................N.....N........ N.............................i................................................. ........................................................N....................... ......................................................N...................N..N.. ..........N....... Pos Score Pred ------------------------ 41 0.402 - 46 0.258 - 90 0.243 - 120 0.201 - 133 0.287 - 166 0.131 - 222 0.792 Yes 274 0.385 - 316 0.444 - 331 0.389 - 388 0.149 - 445 0.125 - 502 0.432 - 529 0.152 - 535 0.121 - 547 0.117 - 575 0.495 - 588 0.375 - 591 0.644 Yes 648 0.225 - 670 0.638 Yes 757 0.230 - 777 0.827 Yes 792 0.570 Yes 814 0.147 - 889 0.125 - 898 0.109 - 904 0.109 - 912 0.860 Yes 985 0.119 - 997 0.167 - 1051 0.489 - 1060 0.680 Yes 1114 0.216 - 1266 0.318 - 1272 0.392 - 1281 0.459 - 1311 0.553 Yes 1417 0.337 - 1495 0.054 - 1515 0.059 - 1518 0.079 - 1531 0.061 -

Explain the output -- Processing locus 446: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 447: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 448: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTCGAAGCGATGCCAGCCCAGATAAGTAAAAATGACTCTCTGCTTTGTTTAAAAAACAAAACCCGGTCAATTCCGGTCG GGTCGGTTCGGGTCAAACTAGTTATTATTTCGGATTTGGTTTATGTACTGTGAGAGTGAGAGATTTTTAGTAAGCAATCC CTCTCTAGTAATGGCGGTCGCAATACCTCAAGCCACTTCTCTTACGAACCTCGTCTTCCTCACGAAATTAATCCCACGAG GAAGCTTCACCACCATACTACCTAAGCATCATCGGAAACCAATCTTCGTATTCTCTCTCCAGACTCAATTTTCTTCTCCA GTAACCACACTCACCTCCAAAAACCCTAAAGATGATGAGCAACAGCAAAAACTCTCCTTCACCCTCAACTACCTTATCGA CTCATGTGGACTATCTCCAGATTCCGCCACCGTAGCCTCTCGTAAGCTCTTACTAGATTCACCAGAACGACCCAACACAG TTCTCAACCTTCTTCGTGACCATGGATTCACCACAGCTCAAATCTCATCTCTCGTCAAGAAACGTCCTGTTCTCTTATTA GCCAATGCTGAATCTGTCCTCCTCCCGAAACTCAGCTTCTTTCTCTCAATTGGTGTCTCAAAATCTTTACTTGCTCGAAC TTTAGCTTCTGATCCAACCATTTTGACTAGATCCCTTGTTAATCAGCTTATCCCTTCTTACAATTTCCTTAAAAGCGTTC TTGATTCCGATGAGAAGATTGTGGCGGCGTTGCGGAGGACGACTTGGGTTTTCCTTGAGGATCACACTAAGAACCTTGTC CCAAACATCAACTATATGGCGGAGACTGGTGTTCCTGAGAAATGTATCAAGCTGCTTCTCACGCATTTTCCTGAAGCTGT GATGCAGAAGAACCATGAGTTCCAAGCAATTGCTAAGCAAGCTCAAGAGATGGGGTTCAATCCTCAAAAATCAACATTTG TGTTGGCCATTCATGCTCTCTCTGGGAAAGGAAACAAATCGATATGGGACAAATGTTTTGAGGTTTATCAGAGATGGGGA TGGTCGGAAGATGATATCATGTGTGCGTTCAAGAAGCACCCTCATTGTATGATGTTGTCTGAGAGGAAGATTAACAGAAC GATGGAATATTTTGTGAATGAGATGAATATGGCGCCTAGATCGATCGCACAGTGTCCAGTGGTGCTCTTCTTCAGCCTTG AGAAGAGGATTATCCCGAGGTGTTCGGTTACTAAGGTTTTGGTATCGAACGGGCTTGTGAAAGAGGATTGGAGCTTGACG TCTTTGTTGGTTCCAGTGGAGAAAGTGTTCTTGGAGAAGCTCGTGATCAAGTACGAAGAGGAGTTGCCTGAGTTGATGAA TCTGTATCTAGGATGCACCAAACTCTGAGCTTTTGTTTTTGACTGTTTTCATTATCAAATGTCAGATGCTGAGAGACATG AATCTTTGTGAAAAGAGTCTAGTGTGTAATCCAATTTTGGTAGTGAA ..........i.....................N............................................... ..........................................i..................................... ..........i..................................................................... ................................................................................ ...............................i..i............................................. ...N............................................................................ .....................N.......................................................... ....N........................................................................... ................................................................................ .........N...................................................................... ...............i.........................i...................................... ............N..............................i........i....................N.....i ..........N.......i.............................N..N............................ ................................................................................ ...........................................................................N.... ............N.............................................N......N...........N.. ............................................... Pos Score Pred ------------------------ 11 0.709 Yes 33 0.283 - 123 0.596 Yes 171 0.853 Yes 352 0.641 Yes 355 0.528 Yes 404 0.421 - 502 0.278 - 565 0.175 - 730 0.127 - 816 0.751 Yes 842 0.773 Yes 882 0.280 - 895 0.206 - 930 0.617 Yes 973 0.127 - 1004 0.658 Yes 1013 0.724 Yes 1034 0.343 - 1040 0.638 Yes 1051 0.123 - 1059 0.679 Yes 1089 0.225 - 1092 0.353 - 1122 0.572 Yes 1138 0.169 - 1143 0.144 - 1149 0.648 Yes 1356 0.180 - 1373 0.235 - 1419 0.143 - 1426 0.145 - 1438 0.064 -

Explain the output -- Processing locus 449: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTTTCTTGTTCGATTTGGACAAGCACGGTTTATCTAAGCGGTACTTAATATAGATGGGCCTGTGGATTGGGCTTGAAGT GGGCTTATAGTGGGCGTGTAGGTGTTGTTATTGGCTTACATGAATAATGGTGCGTTTGTCCCGCCACTCTCTGGTAGACC AAATTATGCTTCACATTTCAAAATCCCTTTTTTGTTGCATCGAAGATCGGGAAGGTCAGAATTGTTTGGGTTTTTGAATC GAATTTTGAGATATTTGAAGGTGGGCATCTGTGAAATTGTGATGAGTTCGGCGAATTTGGAAGATTTGAACAAGGTCTGG GAAATAAAGGCTTTGAAGAGGAAACCTAGAGAAGATGAAGCGAGGAAGATTCTGGAGAAAGTAGCTAATCAGGTTCAGCC GATTATGACTAGACGAAAATGGCGCGTCAAGCTTCTCTCTGAATTCTGCCCAACAAATCCAAGGCTTTTGGGGGTGAATG TAAATAGAGGCGTGCAAGTGAAATTGAGGCTTAGGAGGGTAAACCATGATTTAGATTTCTTATCGTATCATGAAATTCTC GACACCATGCTCCACGAGCTTTGCCACAACGCTCATGGTCCTCATAACGCAAGTTTCTATAAGCTTTGGGATGAACTTCG AAAGGAATGCGAGGAGTTGATGTCAAAGGGTATTACAGGGACGGGACAGGGATTTGATATGCCTGGTAAGCGTTTGGGTG GACTTTCTCGTCAGCCTTCTCTATCATTTCTCAGAGCAACTGCAGCTACAGCAGCAGAAAAAAGAGTGCGTGCGGGAACT CTTTTACCTTCAGGACCTCAACGTCTTGGTGGTGATAGCAGCATTATGTCAGATCTTAGTCCAATTCAGGCTGCTGCAAT GGCTGCAGAAAGGCGTTTACTTGATGATATTTGGTGTGGTTCCCAATCCACAGATGCTTTAGAAGATGAAGAGAATGATA GTGACACATATAAGGAACCTGTTTCCATCAGGGAAACTTGTACGAGCGTGAATGGAAAGTCAGTAAAACGATGTAATAGC TGGTCCAATGCCCATTCTTGTCCTCCATCTTCCAGTCATCAACAGGGATCAGATGTCATTGATTTGACTGAAGAATCTTT TGAAATAAGATGTACTAAAAGAAATCGCAGCCCAGGTGATCAGGGTCCTTCTTGTGCCAAAGATGTACCGAACGCTGGTG TCATGAAGTCATCGATTCCTTTGCCATCGACGAGTTACAATGCAAACCAAAGCAGAGAAGAATCCACAATGTGGGAGTGT GCAGAATGCACCTTATTGAACCCGTCATTGGCTCCAATATGTGAGCTATGCACTGCCGCAAAGCCAAAGGAAAGGGAGAT GAAGCACAAAGTCTGGTCTTGCAAATTCTGCACGCTTGAAAATGAGGTGAAGTTAGAGAAATGTGAGGCTTGTGGTCAGT GGAGGTACTCGTATGGAGCACCATTGTCAACTGGTGCTCCTAATGTCGGCACTTGAGGGAGTTACCAGTCTTCCACAAGG ACTAAATGTCAAACTCTTAGCCATTTTTGGTAGCCAACGGACATGATTCTCCAAGTTCTATAAACAGTGTCCAAA ......................................................N......................... .......................................N......N................................. .....N.......................................................................... .........................................N...................................... ..................................N............................................. ....N.............i..........................................................N.. .............................................N.......................N.......... ......N...........................N...................................N......... ......i............N......................................i..................... ................................................................................ .............................................i................................i. .......................N.............................N...........N........N..... ...................................................N..................N......... .......N............................................i........................... .........N....................................................N................. ..i....................................i............................i........... .....i................................N........N..............................i. .........................................i..................N................... ............N.............................N..................................... .....N....................................N................................ Pos Score Pred ------------------------ 55 0.143 - 120 0.335 - 127 0.284 - 166 0.308 - 282 0.322 - 355 0.229 - 405 0.165 - 419 0.793 Yes 478 0.278 - 526 0.250 - 550 0.437 - 567 0.361 - 595 0.195 - 631 0.187 - 647 0.597 Yes 660 0.445 - 699 0.595 Yes 846 0.825 Yes 879 0.875 Yes 904 0.115 - 934 0.216 - 946 0.161 - 955 0.246 - 1012 0.380 - 1031 0.134 - 1048 0.244 - 1093 0.625 Yes 1130 0.407 - 1183 0.375 - 1203 0.624 Yes 1240 0.743 Yes 1269 0.852 Yes 1286 0.620 Yes 1319 0.428 - 1328 0.236 - 1359 0.630 Yes 1402 0.698 Yes 1421 0.416 - 1453 0.261 - 1483 0.270 - 1526 0.112 - 1563 0.054 -

Explain the output -- Processing locus 450: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATACTCAAAACTTGGAAGTAAAGGTAAATTAAAATATGATTGTTAGAAACAATTACACCAATAATAAAACGACACGTGTA CACTACGTAGACGTTAGATAACCACAGCAAAAGGATATGTATTCTCGAGGCGGGAGAGAGAAAGAGAAGAGAGTGAGAGA GATCGAAGAACATTTACACACATCAATGGCGTCTCTGCAACTCTGCGACGGTTATCTTCTCTTCAAGCCCTCTGTTTCTC CTCGATTCCTCTCTCAACGCATTTCTCATCGCCTAATCCCTAAAGCCTCATCTTCTCCTCCTCCATCTCCATCACCATCA TCGTCCTCTTCATCCTTATCTTTCAGTCGGCGGGAGCTTCTGTACCAATCGGCAGCTGTATCACTCTCGCTTTCTTCCAT TGTTGGACCAGCGAGAGCTGATGAACAGTTATCCGAATGGGAAAGAGTGTTTCTCCCAATCGATCCCGGTGTTGTTCTTC TCGACATTGCTTTCGTCCCCGACGAACCTAGCCGAGGGTTTTTACTTGGAACGAGACAGACTTTGTTAGAGACTAAAGAT GGTGGAAGCACTTGGAATCCACGTTCGATTCCTTCAGCTGAAGAAGAAGATTTCAATTATAGATTCAATTCGATTAGCTT TAAAGGCAAAGAAGGATGGATTATTGGCAAACCTGCGATTTTATTGTACACTGCTGATGCTGGAGAGAATTGGGATAGGA TTCCTCTAAGTTCTCAGCTTCCTGGAGATATGGTGTTTATAAAAGCGACAGAAGATAAGAGTGCAGAGATGGTTACTGAT GAAGGTGCTATTTATGTTACTTCAAACAGGGGATATAACTGGAAAGCTGCTATTCAGGAAACTGTTTCAGCTACCTTGAA CAGAACAGTATCCAGTGGAATCAGTGGTGCTAGTTACTACACGGGAACTTTCAGTGCTGTTAATCGTTCACCTGATGGAA GATATGTCGCTGTTTCGAGCCGTGGTAACTTCTTTCTGACATGGGAGCCTGGGCAGCCTTACTGGCAACCACACAATAGA GCTGTTGCTAGAAGAATTCAGAACATGGGATGGAGAGCTGATGGTGGTCTTTGGCTTCTTGTTCGTGGTGGAGGACTTTA TCTTAGCAAAGGTACTGGGATTACAGAGGAGTTTGAAGAAGTTCCAGTACAAAGCCGTGGCTTTGGCATTCTAGATGTTG GATATCGCTCAGAGGAAGAAGCATGGGCAGCAGGAGGCAGTGGCATACTACTGAGAACAAGAAATGGAGGCAAATCATGG AACCGTGACAAAGCTGCTGATAATATCGCAGCTAACCTATACGCAGTCAAATTTGTGGATGACAAGAAAGGATTTGTGCT TGGCAACGATGGAGTCTTGCTCCGATATGTTGGCTGAAGAATTTAAAAATAAAAAAGAGGTTAAATTGAAATGGTTATGT TTCAATCCTTCTCTGTAAAGCCTTTAACTGCGTTTTAATACTACATTACACTATTG ...................................i............................................ ....................................i........................................... .........................i...................................................... ................................................................................ ................................................................................ ....................N...............i........................................... ..............................................................................i. ................................................................................ ...............N........................................N....................... .............................i......................................i.........N. .............N.................................................................. ..........................................................................N..... ...N....................................N....................................... ........................i....N..........N....................................... ..........................................................................i..... ......................i........................................i............N... ..........................................................N..................... ........N.................N...........................................N.....N... ........................................................ Pos Score Pred ------------------------ 36 0.579 Yes 117 0.540 Yes 186 0.547 Yes 421 0.283 - 437 0.580 Yes 559 0.806 Yes 656 0.402 - 697 0.143 - 750 0.730 Yes 789 0.796 Yes 799 0.246 - 814 0.084 - 955 0.343 - 964 0.277 - 1001 0.246 - 1065 0.762 Yes 1070 0.326 - 1081 0.371 - 1195 0.575 Yes 1223 0.548 Yes 1264 0.691 Yes 1277 0.166 - 1339 0.181 - 1369 0.311 - 1387 0.139 - 1431 0.130 - 1437 0.044 -

Explain the output -- Processing locus 451: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAACGGAAACTTGATATTTTCTGATCTTCGTAACGTCTCTTGTTCTTACAATTTCTCACCTTCTTTAGAGAATTCAAGCA ATTTTGAGCTTCTACGACTAGGTTATACATTTTCGTGAAATTAGGTCGATTTTTGGATACATTATTGGTTTATGTGTTAA TTCAAGGCAGTTGAATCACTTGATGTTTCAGGTACTGTTGTTTATCTCTATGGATGCCAAAAAGTTGGATACTGGCCCGA GAGATGCAATCAACTGGCTCCCTGACGAGATTCTGGGCAAAATCTTGTCATTACTTGCGACAAAACAGGCTGTTTCAACA TCGGTTCTTTCTAAAAAGTGGAGGACTCTCTTTAAATTAGTGGATACCCTCGAGTTTGACGATTCTGTCTCTGGAATGGG TGAACAAGAAGCGAGCTATGTGTTTCCGGAGAGCTTTAAGGATTTGGTGGATCGAACAGTAGCTTTGCAGTGTGATTATC CGATCAGGAAGTTGTCACTAAAATGTCATGTTGGCAGGGACGATGAACAACGAAAGGCTTGTGTGGGCCGCTGGATATCG AATGTCGTAGGGCGCGGTGTTTCAGAAGTGGTACTAAGGATCAATGATCGTGGTTTACACTTTCTGTCTCCTCAGCTCTT GACATGCAAAACACTGGTTAAGCTGACACTAGGCACACGTCTCTTTCTTGGAAAGCTTCCTTCATATGTGTCACTTCCAT CTCTTAAGTTTCTCTTCATCCATAGCGTCTTCTTTGATGATTTTGGAGAGTTGTCTAATGTGCTTCTTGCTGGTTGCCCG GTGGTCGAGGCGTTATATCTCAATCAAAATGGCGAATCAATGCCTTACACCATATCGAGTCCTACGCTCAAGAGACTATC AGTTCACTACGAGTATCATTTTGAGAGCGTAATCTCATTTGACCTGCCAAATCTCGAGTACCTTGACTACTCTGATTACG CCTTGTATGGGTATCCACAAGTTAACTTGGAATCCCTTGTTGAAGCTTATCTGAATCTTGATAAGGCTGAACATGTCGAG AGCCCGGATGTAACCAAACTCATTATGGGAATAAGAAATGTTGAGATCCTTAGTCTATCTCCCGATTCGGTTGGAGTGAT CTATTCATGCTGTAAATATGGGCTACTTCTACCGGTATTCAACAATCTGGTTAGTTTATCTTTTGGGACTAAGAAAACAC GAGCTTGGAAATTGCTTGCAGATATACTTAAACAGTCTCCAAAGCTTGAAACTCTCATCATCGAGGATCTGAATGGTTAC CCACTCGATGTCTCCATGCCTCTGAACCAAGTGAAGGAGTTGCAGATTCTGGAATATGGAGAAAGTGATGACGAGGTTAA ACGGTTGAAGAGTTTTCTTGGGGAAGAAAGTATGATAGAAGTTGTGTTTCCTGAAGTTTGATTGCAAACGAAAAGGGACT TCCTGGAGTTTGAGATCAAAGTACTACTACATTTCATCTAGTTTATTTTCATATAATTCTGTGCAACTTTTAACTTATTA ................................................................................ .......................................................................N........ ......................i..........................N...N.......................... ...N............................................................................ ...........................................................................i.... .................N.............................................................. ......................i....N..............N..................................... ...N.............................................................N.............. ....................................N....................N...................... ............................i..........i........................................ ................................................................................ ......N.................................................................N....... .......N................N............i.......................................... ......N..........N.............................................................. ........................................................................N....... .......N.......i.......................................N...........N............ ...............................N................................................ ................................................................................ Pos Score Pred ------------------------ 152 0.336 - 183 0.651 Yes 210 0.489 - 214 0.169 - 244 0.217 - 396 0.718 Yes 418 0.403 - 503 0.580 Yes 508 0.243 - 523 0.307 - 562 0.230 - 604 0.126 - 644 0.255 - 706 0.223 - 757 0.347 - 778 0.318 - 829 0.669 Yes 840 0.759 Yes 967 0.140 - 1033 0.358 - 1048 0.152 - 1065 0.191 - 1078 0.633 Yes 1127 0.417 - 1138 0.393 - 1273 0.389 - 1288 0.441 - 1296 0.637 Yes 1336 0.288 - 1348 0.350 - 1392 0.302 -

Explain the output -- Processing locus 452: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGATCAATCCTTTTATACATAAAAGAAGTTAAACATTGCATTAGAAATCGTCAGAGTTATTACCATCGGCGAATGCAA ATTTGAAGAGCAGAACGATAAGAAGAAGCGCTCCGAGAGAGAAGAAGAGTAGAGTCAGCATTTTCTCGGATTGAAGCAAC CAGCGAGAGACCAAGTCTTCTTACCCGTGGGCAAAATATGTTGACAGAAATAAATGTAGGAGTAGAACATAAATTATTAA TATATAGGGACCTGTATGCAATTAATCTTATATACCAAAATCTTATTACATCCGAATGCCATTGTTCCTATCATTGTGTT GTCTTTCGATCAAAAAACCCCAAATCTCTGTTTGCAGAGAGGACGAGGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG AGAGAGAGAGAGAGAGAGAGAGAGAACGACTATGGACGCTATAAGGAAGCAGCTTGATGTGCTCATGGGAGCTAACCGAA ACGGCGACGTTCAGGAGGTGAATCGCAAATACTACGACCGTGATGTGTGTCGTCTTTACTTGTCTGGTCTCTGCCCTCAC GATCTCTTCCAATTGACGAAAATGGATATGGGACCATGCCCAAAGGTGCACTCTTTGCAGCTTCGGAAAGAATACCGAGA AGCAAGGGCAAAAGGTGTTGATAACTACGACAGGGAATTGGAAGATGCCATAGACAGGCTTATCGTTGAGTGTGATAGGA AGATTGGTAGGGCCCTTAAGCGTCTTCAAGAAGAGGACGCCAAAGCTGCCATTGCCATTTCTGTCTCTGAAGTCACTCAG TCGCCTGAAATTCTCGAGTTATCGGAGAAAATCAAAGAGAAGATGAAGGAAGCAGATATCCACGATCTTGAAGGCAAAAT GGATCTTAAGATTAGAGCTCTTGAGTTAGTCGAAGAGATGAGGACCAAGAGAGCTGACCAACAGGCGGTGTTGCTGTTGG AAGCCTTCAACAAAGATAGAGCATCCTTGCCACAGCCCGTTCCAGCTCAACCACCATCTTCCGAATTACCTCCACCGGAT CCTCGCACTCAAGAAATGATAAATGAGAAACTGAAGAAGGCAGAAGATCTTGGTGAACAAGGAATGGTTGATGAAGCCCA GAAAGCCCTGGAAGAGGCTGAAGCTCTTAAGAAGCTTACAGTTAGACGAGAACCTCCAGCAGATTCAACGAAGTACACCG CTGTTGATGTGCGCATCACAGACCAAAAGTTGCGTCTATGTGACATATGTGGAGCATTCTTGAGCGTCTATGACAGTGAT CGCCGTTTAGCTGATCATTTTGGGGGGAAGCTTCATCTAGGATACATGCTAGTCCGGGACAAATTAACAGAGCTTCTGGA TGAGAAGGCCAATATACGCAAGGAAAGATCAAAGGAACGGAACAGTAAGGAGAGAGAATCAAGTAAAGACCGAGAGAAAG AACAAGAAACTAGTCGTGAACATCGAAGAGATTATGATCGCAGGAGTAGAGATCGAGATAGGCACCATGACCGTGATCGT GAACAAGACAGAGACTATGATCGGTCACACTCAAGAAGCAGACGTAGGTCACGCTCACGGTCCAGGTCAAGAGATAGACC AAGGGACTATGATCGCCACAGGCGTCACAACCGCTACTAGATCGATCGGTCTTGCTGTCTAACAGGGTTTACAAGTTACA AATGGTTTTAGGATCTATCACCGAAACTTTTTTCTGTCTACATGTATGTTACTCAAGTA ..........................................................................N..... ................................................................................ .....................................N...............N.......................... ...............N.......................................i........................ ................................................................................ ...............................i........................N.......N............... ..........................................N..................................... .....................i.....i.......i............................................ ............................................N................................... ................................................................................ ..........................................i...................................i. .....................................N.......................................... ................................................................................ ...............i......N........................................i......N......... ................................................................................ ......N..............................N........N......................i.......... .............................................N.................................N ................................................................................ .................................N................................i............. ................i............................................................... ........i....................................................................... Pos Score Pred ------------------------ 75 0.340 - 198 0.373 - 214 0.273 - 256 0.246 - 296 0.824 Yes 432 0.652 Yes 457 0.243 - 465 0.293 - 523 0.176 - 582 0.683 Yes 588 0.881 Yes 596 0.644 Yes 685 0.204 - 843 0.652 Yes 879 0.764 Yes 918 0.452 - 1056 0.627 Yes 1063 0.211 - 1104 0.820 Yes 1111 0.163 - 1207 0.460 - 1238 0.353 - 1247 0.140 - 1270 0.526 Yes 1326 0.468 - 1360 0.438 - 1474 0.337 - 1507 0.850 Yes 1537 0.748 Yes 1609 0.587 Yes 1682 0.058 - 1722 0.053 - 1726 0.053 -

Explain the output -- Processing locus 453: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTATGCATCAACAAAAAGACAATTCTTTGTAATAAATATCTTCAATTCGTAAATGTCTTGGCCTTTGTCGTTTTCAAAC TTCAACATCAGCATCACGTGATTGGCTAATACAAATAAAATTTTCTCTTTATTTTATTATTTTGCCACAAGCCGTGAAAA GATGGAGAAAATGAATGTCCGTTTTATGATTGTGTTGATGGTAATGTCTCTGGTTCTGGGTTTTTCGTCGGCAGTTGATT TCAGATGGAGGAAAACTGCAGGATTCTCAGATAGATTCACCAGAGCTGTTTCTTCAGTCGTGTTCCCAGTTCATGGCAAC GTTTATCCTCTTGGGTACTATAATGTAACCATCAACATAGGACAACCACCAAGACCTTATTATCTTGATCTTGATACTGG TAGTGATCTCACTTGGCTCCAATGTGATGCTCCTTGTGTTCGTTGCTTGGAGGCGCCTCATCCACTGTATCAGCCTAGTA GTGATCTTATTCCTTGCAATGATCCACTGTGTAAGGCTTTGCATTTGAATAGTAATCAGAGATGTGAGACTCCAGAGCAA TGTGACTATGAGGTTGAGTATGCTGATGGAGGATCTTCTCTCGGTGTTCTTGTTAGAGATGTCTTCTCTATGAACTATAC ACAGGGTCTCCGGCTCACTCCCCGTCTTGCTCTAGGTTGTGGATACGATCAAATCCCAGGGGCTTCGAGTCATCATCCTC TAGATGGAGTATTAGGGCTTGGTAGGGGGAAAGTAAGCATTCTGTCACAGCTTCATAGCCAAGGTTATGTAAAGAATGTT ATCGGTCATTGCCTAAGCAGTTTAGGTGGAGGAATTCTCTTTTTTGGCGACGATCTTTATGATTCTTCAAGAGTCTCATG GACACCAATGTCTCGTGAATACTCAAAACACTACTCTCCTGCAATGGGAGGGGAACTTCTATTCGGGGGAAGAACAACAG GATTGAAGAATCTATTAACAGTATTTGACAGTGGAAGTTCTTACACATACTTCAATTCCAAGGCATACCAAGCCGTAACA TATTTGCTAAAGAGAGAACTAAGCGGAAAACCGTTGAAAGAAGCACGGGATGACCACACGCTGCCTCTATGCTGGCAAGG ACGTAGACCATTCATGAGCATTGAAGAAGTCAAGAAGTATTTCAAGCCTCTAGCTCTTAGCTTCAAAACAGGCTGGAGAT CAAAAACTCTGTTTGAGATACCCCCAGAAGCTTATCTAATCATTTCTATGAAGGGGAATGTATGTTTGGGAATCTTGAAT GGCACAGAAATAGGTCTCCAGAACCTAAACCTCATCGGCGGTACAGTCTTTATATTACATACATTGGCAATTTCCTTATC TTGGTTTCGAGATTAAATCAATGTTTCTCCTTTCTATTGAACAGATATATCGATGCAAGATCAGATGATAATCTACGATA ACGAGAAACAATCAATCGGGTGGATGCCAGTGGAT ...N.................................................i.......................... ................................................................................ ....N...................................................................N....... ......................N......................................................... .....................N....N..................................................... ..................N..........................................N.................N .......N...........N.....N................................N..........N.......... ................................................................................ ...N..............................................................i........N.... ..........................................................N..................N.. .......i...................................i.................................... ................................................................................ .................................................N..................N........... .............N.................................................................. ...............................................N.........N...N................N. ................................................................................ ....................N...............................N...........N............... .......................N........... Pos Score Pred ------------------------ 4 0.191 - 54 0.599 Yes 162 0.748 Yes 171 0.674 Yes 175 0.363 - 186 0.337 - 198 0.218 - 204 0.488 - 245 0.287 - 313 0.280 - 343 0.139 - 422 0.401 - 427 0.101 - 499 0.261 - 542 0.305 - 560 0.302 - 568 0.174 - 580 0.201 - 586 0.256 - 619 0.407 - 630 0.334 - 724 0.218 - 787 0.529 Yes 796 0.294 - 859 0.238 - 878 0.388 - 888 0.650 Yes 924 0.813 Yes 1090 0.353 - 1109 0.142 - 1134 0.335 - 1248 0.465 - 1258 0.252 - 1262 0.410 - 1279 0.490 - 1381 0.176 - 1413 0.130 - 1425 0.054 - 1464 0.039 -

Explain the output -- Processing locus 454: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAAAAACTACCAGAGAGATTCACATTGACAAAATCAAATGATGTAAAAAGGAATCGTTGGTTTGAAGTTTGAACCAAAC CAGAGATTTGGTTTGGAGTAGACCGGAACCACTGACCTATTGACTAAAAGAGTCAAAGGATGCAATCTTGTGACGCGTGT AATCAACGCGCATGACCTATGGTCCGTATTAAAAAGGTTACTCTATATGTATCACACTTTAGCTTCTCCATTCCCAATTG TCTCTTCTTTTCTTTTTGTACTTGTCAAAAACAAAAAGAACAACAAAAAAAATCTCAACCGTAGAAAATTCCGACAAGAG TTCAGTTCATACAATGAACTAAGTATGGGTTTCTTTGGAAGACTGTTCGGAAGTAAGAAGCAAGAAAAGGCAACACCGAA CAGACGAAGATGGAGCTTCGCTACTAGATCCTCACATCCCGAGAATGATTCGTCTTCTCATTCAAGCAAGAGACGTGGGG ATGAAGATGTCTTAAACGGCGACAAGCATGCGATAGCCGTCGCGGCTGCTACAGCTGCAGTGGCTGAAGCCGCACTCGCT GCTGCTCGTGCGGCGGCGGAAGTCGTGAGACTCACCAATGGTGGTAGAAACTCGTCGGTAAAACAAATCAGTCGGAGTAA TCGTCGGTGGTCTCAAGAGTATAAAGCAGCTATGAAGATTCAATCCGCTTTTCGTGGCTACTTGGCGAGGAGGGCGTTGA GAGCACTGAAGGCATTAGTGAAGCTTCAAGCGTTGGTGAAGGGACACATAGTAAGGAAACAAACGGCTGATATGCTGCGT CGAATGCAAACGCTGGTTCGGCTCCAAGCACGAGCTAGAGCTTCGCGTTCTTCTCACGTTTCTGACTCTTCCCATCCGCC AACACTAATGATTCCATCTTCCCCACAATCTTTCCATGCACGATGCGTTTCAGAGGCTGAGTACAGTAAAGTCATTGCCA TGGATCACCACCACAACAACCACCGTTCACCGATGGGTTCAAGCCGGTTATTAGACCAATGGAGGACAGAGGAAAGTCTA TGGAGCGCACCAAAGTACAATGAAGATGATGACAAAATCCTAGAAGTCGACACTTGGAAGCCTCACTTCAGAGAGTCACC AAGGAAAAGAGGATCTCTAGTGGTTCCTACAAGTGTGGAGAACAGTCCACAATTAAGGTCTAGAACAGGAAGCAGCAGTG GTGGTTCAAGGAGAAAAACTCCCTTCACGCCTGCGAGAAGCGAGTACGAGTACTACTCTGGGTATCACCCTAACTACATG GCTAACACTGAGTCTTACAAAGCAAAAGTCCGATCACAAAGCGCACCAAGACAGAGACTACAAGATTTACCTTCAGAGAG TGGTTACAAGAGGTCTATACAGGGACAGTATTACTACTACACGCCTGCTGCAGAGCGATCGTTTGATCAGCGTTCGGATA ACGGGATCGCGGGTTACAGAGGAGTTTCTGATGGGTTAGATCGAAACCAAAGTGACAAATCGAAGATGTACACTTCGTTT TTCAGTTCTAATCCTCTTTTCTTTCAATAGTCGAGAAAGGATGAAAAAAGTGAGTGGAATGTGTAAAATTAGATTTCGAC ACACGAGTACAGAGACAGCCAGTGATCAATCTGTGTTTTGTACTATTTT ......................................i..i...................................... ...........................................................N.................... ...........N......N...........................N................................. ................................................................................ .............i..........N....................................................... .........N..................................N................................... N.....N....................N.................................................... .....................................i.......................................... ...............................N................................................ .......................................................................N........ ...N............................................................................ .......i...........................N......N....................................i ................................i.........................i....................N ...................N.....N..N................................................... ................................................................................ .............................................................................N.. ................................................................................ ................................................................................ ..............................N..................................i.............. ........................................N.................N..................... ................................................. Pos Score Pred ------------------------ 39 0.653 Yes 42 0.738 Yes 140 0.402 - 172 0.223 - 179 0.293 - 207 0.294 - 334 0.602 Yes 345 0.384 - 410 0.328 - 445 0.355 - 481 0.256 - 487 0.388 - 508 0.261 - 598 0.534 Yes 672 0.460 - 792 0.329 - 804 0.322 - 888 0.545 Yes 916 0.426 - 923 0.268 - 960 0.532 Yes 993 0.793 Yes 1019 0.636 Yes 1040 0.228 - 1060 0.364 - 1066 0.462 - 1069 0.426 - 1278 0.321 - 1471 0.406 - 1506 0.671 Yes 1561 0.243 - 1579 0.064 -

Explain the output -- Processing locus 455: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGAGTCACTAGATCAAATGTGACCACATGATGGAGAAGCCCATCCACTTGTGAAGCCCAAGTGAGAAAATCAAAAATATG TGTGAATCATAGAAAACGTGAAGAGGGAAGAGAGATAAAACCACAACCATACAAATGAATGACAAAGCTATCCAGAATCT CATCTTCTTTGCCTCCAACTCAGCAATTCATATTTTCCTATATTACAACTCTCTGCCTCTTCTGCTTACATCATACAACA AGCTTCTCTCTCTGGTAGCCATGGCTTCGGCTACTTTCTCTGTGGCCAAACCATCTCTTCAGGGTTTTTCTGAGTTCTCA GGACTTCGAAACTCCTCTGCTCTTCCCTTTGCCAAGAGATCTTCTTCCGATGAGTTTGTTTCCTTCGTCAGTTTCCAAAC TTCTGCAATGAGAAGCAATGGTGGATACAGGAAAGGGGTGACCGAGGCCAAGATAAAGGTAGCCATCAATGGGTTCGGTA GGATTGGTAGGAACTTCTTGAGGTGTTGGCATGGTCGTAAGGACTCTCCTCTTGATGTCGTTGTCATTAACGACACTGGT GGTGTTAAACAAGCATCACATCTCCTCAAATACGACTCAACTCTTGGAATCTTTGACGCTGATGTCAAACCTTCAGGAGA CTCAGCTCTCTCTGTTGATGGAAAGATCATCAAGATTGTATCTGATCGTAACCCATCTAATCTCCCCTGGGGGGAACTAG GCATTGACTTAGTTATCGAAGGAACCGGAGTGTTTGTTGACAGAGACGGTGCTGGGAAGCACCTTCAGGCTGGAGCCAAG AAGGTTCTTATCACTGCACCTGGAAAAGGTGACATCCCAACTTATGTCGTTGGTGTCAATGCTGAACTTTACAGCCATGA AGATACGATCATCAGCAATGCGTCTTGTACTACTAACTGTCTCGCTCCATTCGTCAAGGTTCTTGACCAGAAATTTGGGA TCATAAAGGGTACAATGACAACTACTCACTCATACACTGGTGACCAAAGGTTGTTAGATGCGAGCCACCGTGATCTAAGG AGAGCAAGAGCAGCAGCTTTGAACATTGTTCCAACATCTACAGGAGCAGCCAAAGCTGTGGCTCTTGTGCTTCCTAACCT CAAAGGAAAACTTAACGGAATTGCATTGCGTGTGCCAACTCCAAACGTTTCAGTGGTTGACTTAGTCGTGCAAGTCTCCA AGAAAACTTTTGCTGAAGAAGTCAATGCTGCTTTCAGAGATGCAGCTGAGAAAGAGCTTAAAGGTATCCTTGACGTCTGT GATGAGCCTCTTGTCTCTGTTGACTTCAGGTGCTCTGATGTATCCTCCACTATTGATTCTTCCCTCACAATGGTTATGGG AGATGATATGGTTAAAGTGATTGCTTGGTATGACAATGAATGGGGTTACTCACAGAGAGTCGTTGATTTGGCTGACATTG TTGCCAATAACTGGAAGTGAAGACTCAACAACCTCTTGATGTCTGCTCCATTATTTTTTACTGTTACATGATTCGGAATG TATAATTGTAGTTCCTGAGTTTATTATGTATTTGTGCTC ................i.........i..i...............................................i.. ......................................................N...N..................... ................................................................................ ....................i........................................................... .................................................N.............................. .......i.........i..................................................N........... ..............................N.......................N......................... .............................................................N.................. .................N.............................................................. ................................................................................ ...........................................N..............N.................N... .................N.............................................................. ..............i..........................................N...................... ................................................................................ ................................................................................ ........................N..............N........................................ ..N....i.....................N.....N...i........................................ ......................................N............................N.........N.. .........................N............. Pos Score Pred ------------------------ 17 0.805 Yes 27 0.705 Yes 30 0.689 Yes 78 0.543 Yes 135 0.332 - 139 0.322 - 261 0.689 Yes 370 0.236 - 408 0.604 Yes 418 0.544 Yes 469 0.403 - 511 0.248 - 535 0.158 - 622 0.170 - 658 0.210 - 844 0.190 - 859 0.118 - 877 0.132 - 898 0.189 - 975 0.744 Yes 1018 0.212 - 1225 0.139 - 1240 0.153 - 1282 0.058 - 1318 0.227 - 1350 0.780 Yes 1356 0.722 Yes 1363 0.486 - 1368 0.768 Yes 1390 0.144 - 1396 0.167 - 1400 0.669 Yes 1479 0.073 - 1508 0.051 - 1518 0.082 - 1546 0.058 -

Explain the output -- Processing locus 456: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTATTAAATGTTGTTATTTATGGGTAAAGCATAAATACTTAAATTCAATTATTATAAGAAAATAAATTAAAACCCGACC CCATATTTTTCGTCCTTTTAAAAGATGGAGTCCTCGAAAACATTACGTATTAGTAAGTTCCATGCAAAGGACAAACATAT AATAACAACAACTTCTCTTCTTTACTCGTTTCTCTCTCTCTCTTTTCTTTTCTTTTCTTCAAGCGCTTCTTTAATGGCGG CTGTTCAATGTCTCACTTTCCTCTTCTTGTTTCTTCTCCAAAACGCGACGTCAGCTTCACCTCTCCCTCTCTTCCGGCGG CCGATCCAAAGCAACCACAGCAACTTCGTGAAGCATCCTCGCCGAAGTCAGGTGGTTTTCCCGGTGAATCATAGTAGTTG TGATCTGTTCGCCGGCGAGTGGGTGCGGGATGAGACTTACCCACTTTACCGGTCTAAAGAATGCGGCCGAGGAATCATAG ATCCGGGATTCGACTGCCAGACTTACGGCAGACCTGACTCCGACTATCTCAAGTTCCGGTGGAAACCTTTCAACTGCAAT GTCCCTAGATTCAATGGAGTGAAGTTTCTACAAGAAATGAGAGACAAAACCATAATGTTCGTTGGTGATTCGTTGGGTAG AAACCAATGGGAGTCGTTGATATGTATGATCTCTTCATCAGCACCGTCCATTAATACTCACATCATTCATGAAGATCCTC TCTCTACCTTCAAGATCCTTGACTACAACGTGAAGGTATCATTCTATAGAGCTCCTTATCTTGTAGACATCGACAAAATT AACGGAAAGACAACTCTTAAACTCGACGAAATCTCTGTGGATGCATCGAATGCTTGGCGCACAGCCGACGTTCTATTGTT CAACACTGGTCACTGGTGGAGCCACACAGGTTCTTTACGAGGGTGGGAGCAGATGGAGACAGGTGGGAGATATTACGGCG ACATGGATAGGTTAGTGGCGTTGAGAAAAGGACTAGGAACGTGGTCTAGTTGGGTCCTTCGTTATATCAATTCTCCTCTC ACTAGAGTTTTCTTCCTCTCGGTCTCACCCACACACTACAACCCAAATGAGTGGACTTCAAGATCAAAAACTTCAACAAT AACTCAAGGAGGTAAAAGCTGCTACGGACAAACGACACCGTTTAGTGGAACAACGTATCCAACAAGTTCATACGTGAACC AGAAGAAAGTGATAGACGACGTGGTTAAAGAGATGAAGTCTCATGTCTCATTGATGGATATAACTATGCTCTCTGCTCTT CGTGTCGACGGTCATCCTTCGATATATAGTGGAGATCTCAACCCTTCTTTGAAAAGAAATCCTGACCGTTCATCTGATTG TAGCCATTGGTGTCTTCCGGGTCTTCCCGACACTTGGAACCAATTGTTTTATGCTGCTTTGTTGTACTGAAGATTTGATT AACCAAGATCAAACAAAATGTATCTATTGAATTATATAGTTTCCTAATTTTTATTTGTAAGTTCACTAAGTTGTTACGTT TAATCAAAC ........N...........N........................................................... ........................N....................................N.................. .........................................................................i...... .......i........................................................................ ................................................................................ .............................i..............................N................... ..............................................................................N. .............N......................i.................N......................... ......i..............N...N..........................................N........... ................................................................................ ........................................i........i.............................. ....................................................N........................... ..N............................................................................. ..............................................N................................. ................................................................................ ................................i.........N..........N...........i.............. ................................................................................ ..................................................N............................. .................N.............................................................. ......... Pos Score Pred ------------------------ 9 0.322 - 21 0.293 - 105 0.307 - 142 0.091 - 234 0.623 Yes 248 0.772 Yes 430 0.551 Yes 461 0.445 - 559 0.469 - 574 0.187 - 597 0.514 Yes 615 0.375 - 647 0.618 Yes 662 0.186 - 666 0.149 - 709 0.213 - 841 0.640 Yes 850 0.799 Yes 933 0.279 - 963 0.219 - 1087 0.456 - 1233 0.620 Yes 1243 0.228 - 1254 0.194 - 1266 0.646 Yes 1411 0.126 - 1458 0.185 -

Explain the output -- Processing locus 457: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGCAGTAGTGGGAATGTGGTGGGATACATGTCTTTGTCCTTTGATTTGATATTTCTGGATAAATTTGCTTTCTTTTTTG TTGAAACAGATCACCAAATAAGAAAACTCTTGTGGTTCTATTGAATTGGGTTTCCACTATCTTTATTGTAGAGGTAACTT TATTTTGTGTTTGTTGGTATTGAGTTCTGTACATCCATCAGGATTTTCGTTGTTGTCTGAGCTTTCTTCTATTTTTTTTC TTATGCAGATGGAGTCTTCAAGTCCCCACCATAGTCACATTGTTGAGGTTAATGTTGGAAAATCTGATGAAGAGAGAATA ATTGTGGCGAGTAAAGTCTGTGGAGAAGCACCATGTGGGTTTTCAGATTCTAAGAATGCTTCCGGGGATGCTCACGAACG CTCTGCTTCTATGCGGAAGCTTTGTATCGCCGTCGTGCTGTGTCTAGTGTTCATGAGTGTTGAAGTTGTTGGTGGGATTA AAGCCAATAGTTTAGCTATATTAACCGATGCAGCTCATTTGCTCTCTGACGTTGCTGCCTTTGCTATCTCCCTCTTCTCA TTGTGGGCTGCTGGCTGGGAAGCGACTCCTAGGCAGACTTACGGGTTCTTCAGGATTGAGATTTTGGGTGCTCTTGTATC TATCCAGCTCATTTGGTTGCTCACGGGTATTCTGGTTTATGAAGCGATTATCAGAATTGTTACAGAGACCAGTGAGGTTA ATGGATTCCTCATGTTTCTGGTTGCTGCCTTTGGTCTAGTGGTGAACATCATAATGGCTGTTCTGCTAGGGCATGATCAT GGTCACAGTCATGGACATGGGCATGGCCACGGCCATGACCATCACAATCATAGCCATGGGGTGACTGTTACCACTCATCA CCATCATCACGATCATGAACATGGCCATAGTCATGGTCATGGAGAGGACAAGCATCATGCTCATGGGGATGTTACTGAGC AATTGTTGGACAAATCGAAGACTCAAGTCGCAGCAAAAGAGAAAAGAAAGAGAAACATCAATCTCCAAGGAGCTTATCTG CATGTCCTTGGGGATTCCATCCAGAGTGTTGGTGTTATGATTGGAGGAGCTATCATTTGGTACAATCCGGAATGGAAGAT AGTGGATCTGATCTGCACACTTGCCTTTTCGGTTATTGTCCTAGGAACAACCATCAACATGATTCGCAACATTCTAGAAG TATTGATGGAGAGTACACCCAGAGAGATTGACGCCACAAAGCTCGAAAAGGGTTTGCTCGAAATGGAAGAAGTGGTGGCT GTTCATGAGCTCCACATATGGGCTATCACAGTGGGAAAAGTGCTATTGGCTTGCCATGTCAATATCAGACCAGAAGCAGA TGCAGATATGGTGCTCAACAAGGTAATTGATTACATCCGCAGGGAGTACAACATTAGTCATGTCACGATACAAATCGAGC GCTAAAAGCTAAGTAAGATCTGATGAAGGGTTTTTGTATCAGCATTCTCATTAACAATAAAATCAATAAAGTTTCTACAT TTTTCACTTTCTCGTGTTCCTCTG ..............N.............N................................................... ................................................................................ ................................................................................ ..i.....i..........................................N..............N............. ................................i......................N...........N............ ..........i.........................................i........................... ...........................N.................................................... ................................................................................ ......................................N......................................... N..........i.........................................i..................N.....N. ..........N.....i.....N...........N....................N........................ ..............N.....N...........i.....N.................N.....N.....N........... ................................................................................ ..........................................................i..................... .....i........................................................i................. ....N............i.....................................N.......................N .......i...................................................N.................... ......................N......................................................... ........................ Pos Score Pred ------------------------ 15 0.451 - 29 0.346 - 243 0.537 Yes 249 0.780 Yes 292 0.287 - 307 0.226 - 353 0.670 Yes 376 0.145 - 388 0.067 - 411 0.692 Yes 453 0.537 Yes 508 0.161 - 679 0.096 - 721 0.184 - 732 0.514 Yes 774 0.703 Yes 793 0.228 - 799 0.412 - 811 0.481 - 817 0.603 Yes 823 0.463 - 835 0.212 - 856 0.460 - 895 0.217 - 901 0.312 - 913 0.504 Yes 919 0.288 - 937 0.191 - 943 0.243 - 949 0.102 - 1042 0.134 - 1077 0.332 - 1112 0.738 Yes 1179 0.510 Yes 1206 0.689 Yes 1263 0.747 Yes 1285 0.202 - 1298 0.659 Yes 1336 0.155 - 1360 0.400 - 1368 0.686 Yes 1420 0.369 - 1463 0.394 -

Explain the output -- Processing locus 458: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 459: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAACCTGTAGGTATATACTCTAATATAGGCAGTACATTGATTATATCAAATATCTATGTGATTTGTATACCATTTTTAT TTGAATCATTATCTGTGTTAACTTTTGGAATTGTGTACATTTATCTTTATGATATACAGTGGAAATATGAGAAGACCTAG TCAAATGATGAGGCTTCTATTAACATCCTTTTTCGGTGTTATTGTTGGTTTCCTTATGGGTATTACTTTTCCAACCTTGA CTTTAACTAAGATGAATCTTCCATCCACATTGTTTCCCTCGATTGATCTTGCATACATTGAGGATAAATACTCTGACATA TCAAGACAAAGACTATTTGGTTCTTGGTCTTCGACAAAAGGCCTCAAACTCAAGAATGACATCCCTGACCCTCCATATAA CTATAATGACACTAAGATATGGGTTTCGACTAACCCCCGTGGTGCTGAGAGGCTACCACCAGATATAGTCACGCCTGAAT CAGATTTTTACCTCCGTCGACTGTGGGGCGACCCTAATGAGGATTTAACAGTCAAGCAGCGGTATCTAGTAACATTTACG GTTGGCTATGATCAGAGGAAAAATATAGACACTGTGTTGAAGAAGTTCTCAGATAACTTCTCTATAATGCTGTTTCACTA CGATGGCCGGGCAAGCGAATGGGAAGAGTTTGAATGGTCCAAGCGAGCCATTCATGTGAGCATTCGGAAACAAACAAAAT GGTGGTACGCTAAGCGATTTCTTCATCCTGACATAGTTGCCCCCTATGAATATATCTTCATATGGGATGAGGATCTTGGC GTGGAACACTTTGATTCGGAAAAATATCTGGCGGTGGTGAAGAAGCATGGTTTGGAAATCTCACAGCCTGGATTAGAGCC ATATGAAGGGCTCACATGGGAGATGACCAAGAAAAGAGACGACACTGAAGTCCACAAGCATGCTGAGGAAAGGAATGGGT GGTGCACTGATCCCAATTTACCCCCTTGTGCAGCGTTTGTGGAGATTATGGCTCCTGTTTTCTCCCGCAAGGCATGGCGC TGTGTGTGGCATATGATTCAGAACGATTTGATTCATGGATGGGGTCTGGACTTTGCCGTTCGGAAATGTGTTCAGAACGC ACACGAGAAAATTGGAGTTGTAGATGCTCAATGGATTATACATCAAGGTGTTCCATCATTAGGGAATCAAGGACAACCAG AGCAAGGGAAACAACCATGGGAAGGGGTGAGAGAACGATGCAGGAGAGAGTGGACAATGTTTCAAGACAGATTGGATGAT GCTGAAAAAGCTTATTTTGAAGCATCTGCTCACAAGAATGCTTCTTCACGGCCTCACGGGTAATAAGGAAACATATAGTC TCTCCCTCCGACTTCCACTCTCTTTGTTTACCCTGTTAATTTTGTTCCCGGAGCTGTGCTTTGATTTCGTGTTTATGGAA GA ........................................................N....................... ................................................N.................i............. ....N..N...............................................N........................ ...........N.................................................................... .......................................................i........................ .....N............i............................................................. ....................................N........................................... .......N..........................................................N............. ..i...............N..............N...................N........................i. .............................................N...............i....N............. ..............................................N................................. ..N............N......N....................................N..............N..... ...............................................N.........................N...... ............i.....................N...N..........................N.............. .......................N......i................................................. ................N....................N..................i..................N..N. .....................................N.......................................... ..........................................................................N..... .. Pos Score Pred ------------------------ 57 0.127 - 129 0.131 - 147 0.521 Yes 165 0.302 - 168 0.181 - 216 0.319 - 252 0.324 - 376 0.621 Yes 406 0.324 - 419 0.776 Yes 517 0.185 - 568 0.239 - 627 0.338 - 643 0.501 Yes 659 0.375 - 674 0.244 - 694 0.149 - 719 0.587 Yes 766 0.150 - 782 0.635 Yes 787 0.307 - 847 0.308 - 883 0.465 - 896 0.384 - 903 0.490 - 940 0.350 - 955 0.311 - 1008 0.306 - 1034 0.342 - 1053 0.624 Yes 1075 0.352 - 1079 0.437 - 1106 0.347 - 1144 0.335 - 1151 0.598 Yes 1217 0.450 - 1238 0.298 - 1257 0.560 Yes 1276 0.312 - 1279 0.278 - 1318 0.106 - 1435 0.053 -

Explain the output -- Processing locus 460: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 461: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGTGCGACGTCGTATAGTACGGCTTAAGAAATTTACACGAGCATACAAATTTTCATATAGAATATAAACATTAATTTAG AATTAACTATAAATTAATTAATCCGTTTTGACTTCTTTATTTCTTATTCTAAATTTTGCATTATATACACATCTGTTTGA TCCCTTTATTTGTTTCTATCAAGACCTTGAAAAAATGGCATTAGCATCACATATTCTTATTCTTCTTTCTCTTTTTCTTC TGATTTCCCTCGTCCGACCACAAGTTCCTCCAATGGAGCAATTCCGGTTCCTTAACAACGGCGACTTTGGAGAATCCACG GTGGAGTACGGTGCAAGTTACAGAGATTTAGGTGTAATACGTAACCAATTTCGTCTATGTTTCTTCAACACCACGCCAAA CGCATTCACACTCGCTATCGGGATGGGGACAGGAAGTTCTGATTCAATCATTCGTTGGGTTTGGCAAGCTAACCCACAAA AACCAGTTCAAGAAGAGGCTTCTCTTTCGTTTGGACCGGAGGGAAACCTTGTCCTCGCCCAGCCAGATGGCAGAGTCGTA TGGCAGACCATGACAGAAAATAAAGGTGTTATAGGCTTGACCATGAACGAAAATGGAAATCTTGTGTTGTTTGATGATGG AGGATGGCCCGTGTGGCAGAGCTTTGAATTCCCAACGGATACACTCTTGGTCGGTCAGTCTTTAACACTCGATGGGTCGA AGAACAAGCTCGTAAGCCGCAACAATGGGTCTTATAGTCTTATCTTAGAGCCCGACCGACTCGTTCTAAACCGTTTAATC CCTCGTAGCAACAACAAATCCCTTGTGTATCACATTATAGAGGGTCGGTTTATTCCAAGCGCCACTCTATACTCGGCTAA AGACCAAGGGACCACGACTCAATTGGGCCTAGCCACGCCGGGGCTCAGGCCTGAGTTTCCATATAAACATTTTCTCGCAA GGCCTAGATTCAACGCCTCACAAAGTTTCCTTAGGCTCGACGCTGATGGAAATCTTAGGATTTACTCTTTCGATTCCAAG GTCACGTTTCTTGCATGGGAAGTAACGTTCGAGCTTTTCAACCACGACAACAACAACGAGTGTTGGTTACCGTCCAAGTG TGGAGCGTTTGGGATTTGTGAGGACAATCAATGTGTGGCTTGTCCGTTGGGAGTGGGCTTAATGGGCTGGAGTAAGGCTT GTAAGCCTAAGAAGGTGAAGAGTTGTGATCCGAAGAGTTTTCATTATTATAGACTTGGAGGTGTTGAGCATTTCATGACT AAGTATAACGTTGGTCTTGCGCTTGGAGAGAGCAAGTGTCGGGGTTTGTGTAGTGGAGACTGTAAATGTTTGGGGTATTT TTTCGATAAAAGTAGCTTTAAGTGTTGGATTAGTTATGAGCTTGGGACTTTAGTCAAAGTGTCTGATTCCAGAAAAGTGG CTTATATCAAAACTCCTAATGTGTGATCAGTAACGTGTAGAAAAGGCAAAAAACATAATGGGCTTAAAGTACTAATAAGC CCATTATATGTACGACTTAATAAAGGCCTTTTTATCTATGTAATA N............................................................................... ................................................................................ ..................................i............................................. ................................N............................................... ........................................................N....................... ......................i......................................................... ..................................................................N............N .........i................................N.........N....................N..i... ...i...................................................................N........ ........................i....................................................... ................................................................................ ................................................................................ .............................................N.................................. ..............N................................................................. ..............................N..............................N.................. ..........................................................................N..... .................................................................N.............. ...................................N............................................ ..................N......................................N...................... .......N.............................N....... Pos Score Pred ------------------------ 1 0.201 - 195 0.802 Yes 273 0.359 - 377 0.222 - 423 0.545 Yes 547 0.287 - 560 0.345 - 570 0.709 Yes 603 0.252 - 613 0.240 - 634 0.334 - 637 0.582 Yes 644 0.748 Yes 712 0.434 - 745 0.714 Yes 1006 0.397 - 1055 0.465 - 1151 0.323 - 1182 0.406 - 1275 0.402 - 1346 0.114 - 1396 0.276 - 1459 0.188 - 1498 0.141 - 1528 0.039 - 1558 0.053 -

Explain the output -- Processing locus 462: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTACCGTGTAAGAAAGTGAAATTTTCGGTTCAAGAAGTGAAATTTTCTTTAAGTTGTGTTTTTTTTTTCACTTATGAAG TCATATGATAATTTGATATGAATCTTAAAAAGCTTTTTTTTTTATGACATGATGCAGATAGTGAAGTCTCGGAGACTGAA TGATATTGTTTCTGGTCAACTTAACCAATTTCAGAACATGGCAAACAGAGCAGACAACTCAATGCGTTGTTCAAATAGTA AGAAACCGAATGACGGTATGATGCTCGTAATATCGATTTTCGCGGGAATAGTTATCGGATTTTTATTAGGCATATCTTTC CCTACATTGTCACTAACTAAGATGAATTTTCCATCAAGCATTCTTCCTTCTGTTGCTACTGTCTACATAGAAAATGAGAA ACCGGAGAAATCATCTAAGACGAGTTCACAAAACGAAAGTGATGCATCACATCATCATAAGATTTGGGTTCCATCGAATC CTCGAGGTGCAGAGATGTTACCTCCAAGTTTTGTTGCAGCCGAATCGGATTTTTACTTACGAAGATTGTGGGGATTGCCT AAAGATGATTTACCAGTGGTGAAGCCTAAGTATCTTGTTGCTTTTACTGTTTCTTATGAACAAAGAAAAAACATCGATGC TTGTGTCAAAAAATTCTCAGATAACTTCACCATTGTTCTGTTTCATTACGATGGAAAAACCTCGGAGTATGATGAATTTG AATGGTCTAAACGGGCGATACACGTCAGTGTACCTAAGCAAACCAAGTGGTGGTATGCTAAAAGGTTTCTGCATCCTGAT ATCATAGCACCATATGAATATATATTCATTTGGGATGAAGATCTTGGCGTTGAAAACTTCGACGCAGAAGAGTACATCAA GATTGTGAATAAGCATGGTCTAGAAATATCGCAACCCGCTGTGGAATCAAGAAAATCAATCACATGGAAGATAACAAAGA GAATACCCGGAATCGAAGTTCACAAAGAAGTCGAAGAGAAACCAGGCCGCTGCAATGACCCTCACCTACCTCCTTGTGCA GGGTTTATAGAGATTATGGCTCCAGTTTTCTCTAGAGAGGCATGGCGTTGTGTGTGGCATATGATTCAGAATGACTTGGT TCACGGTTGGGGTCTTGACTTTGCGCTAAGAAAATGCGTCGAGCCTGCACATGAGAAGATTGGTGTTGTGGATTCTCAAT GGATCATTCATCAAAAGATTCCTTCTTTAGGCAGCCAGGGAACCGCGCAGGAAGGGAAAACCGCGTTCCAAGGGGTAAGA GAGAGATGTAAACGGGAATGGACAATGTTTCAGAAAAGAATGACCCGATCAGAGCAGAAGTATCTGAAAGAAATTGCATC TGCCTCTTCAAACTCTACACGTACTTAAACCAAAAGAGTAGAGGAACTGAATAACAACCTTTAGTGTTCAGATAGCACTT TCAGTAAGAAAAAAAGAGAAATAGAATGTTGATATGATAAGCACATA ..........................................................................N..... ....N............N.........................N....i..N...........................N .....................................i.......................N.................. .........N.......N..N........................................................... .....................i...................................................N...... .........................................i...................................... ..............N................................................................. ....i..................................................N....................N... ..................................................N.................N..N........ .............N....................N............................................. ..............N................................................i................ ......................................................N......................... ...............N.........................N..................i.........N......... .................................N................N...........................i. ................................................................................ .....N...........N......i..............N........................................ ................................................................................ .........................N.......N............. Pos Score Pred ------------------------ 75 0.196 - 85 0.102 - 98 0.371 - 124 0.135 - 129 0.503 Yes 132 0.327 - 160 0.120 - 198 0.647 Yes 222 0.312 - 250 0.173 - 258 0.312 - 261 0.460 - 342 0.765 Yes 394 0.456 - 442 0.529 Yes 495 0.422 - 565 0.519 Yes 616 0.161 - 637 0.379 - 691 0.496 - 709 0.365 - 712 0.247 - 722 0.459 - 775 0.204 - 814 0.163 - 835 0.278 - 895 0.225 - 944 0.645 Yes 1015 0.353 - 1056 0.478 - 1082 0.370 - 1101 0.608 Yes 1111 0.265 - 1154 0.475 - 1171 0.242 - 1199 0.563 Yes 1286 0.365 - 1298 0.303 - 1305 0.639 Yes 1320 0.205 - 1466 0.054 - 1474 0.045 -

Explain the output -- Processing locus 463: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 464: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAACAGAGTTTCTATTTGAGTAGAATTGTAAAATAGAATTTGATGTCTGCCAAGTGTTTGATGAAATTCCTCTGAGAA ATGAACCTTAAGTTATTCTAGTGTGTATTAATGGATTTCTTCTTTGTTGTTCAGGTTATTGTGATGGAAGCTGTGAAGGA AGCTGTGAGGAAGATTAAGTCCTTAGTCATACCACATGCAGATGAGAAAGATAATGGTATTGTTTTTGAGATCAAGCTTA ATGAGACAGATCAGAGAGTAGAAAAGTGGGGTCTTGATCCTTCTTTGGATTTTTTCGAGGTAACAGGGAACTGTAATTTG GGACGTCCAAATAGCGAGGGATCTAATCAGTCTCTCATGGGTTCTGTGACCATTAGGAACATCTTTAATCCTAAGCTGGA TGATCTTTTGTCTAAGATTGAGTATGTACGGTTTCTGGAAGCTGTTAAAAAACCTAGAAACCGTACCTTCAAAACGAGCT TCTTCAATAGCAGAAAACTTAGCCCAGTGTTCACTGGAGGACCAGGCTATGAGGATCTAGTGCCTCCAATGTTTGTGGGT CGAGATTGTCTTAAAGCTACTATAACTGAGAACTTAACCCGTCAGAGAGAACTCACATATGGAGTTATGTTTGAAGAGAT AATAACCCGAGATGAAAACAGGCGAATCTCTGAAAATGGGCTCTTGTTATCGCCTGACGGTGGAATCAGTATTAATGGAC CTCCAACAACTCTAAGTGGTACCGGTATTGACCACATTGCCACTCTACAAGCCAACATAACCCGAGACAACACCAAGCTA GTGAATGGGGCTGTTGTTGGAGAGAAGAATATCTTTCAGGTGGATCAAGGCTTAGGTATAGGAAATAATTTTCCGCTCTT CAACCGCCACCAACTATCATTAACAAGTTTCATTCAGCTGAAGCAAGTAGAAGAAGGATCCGATAAACCACAACCACCAG TTCTAGTCCTCCATGGTCGTTACGGTGGCTGCATAGGCGATCTTCCAAGCTATGATGTTTTTGCTCTTGGTGGACCTAAC TCCGTTCGAGGATACAGCATGGGAGAACTTGGTGCAGCTAAAAACATTCTCGAGCTTGGTGCTGAGATTAGAATTCCTGT CAAGAACACACATGTGTATGCATTTGCTGAGCATGGAAACGATTTGGGAAGTTCAAAGGATGTGAAAGGGAATCCAACAG GGCTTTACAGGAAAATGGGACATGGTTCATCGTATGGTTTGGGTGTGAAACTCGGTATGGTGCGAGCTGAATATACTGTA CGTCACAATCGTGGAACCGGTGCTCTGTTTCTCAGGTTCGGGGAGAGATACTAAGGTCATGCCATGAAGACTTTGTGCTG CTTTCTTTCTTAACTCTAATACTTTGGTTTATAATCCTGATACTCTAGTTATGTAAATGCGTGCATAGCAAGT ............................................N.................N................. N.............................i................................i................ ...................................N.....N...........N.......................... N............................................................................... ....................................i..........................................N .......................N........................................................ ................................................N...................i........... ..........................................................N.......i............. ...........N.......................i......................................N..... ................................................................................ ....N........................................................................... ................................................................................ ............i......................................N..N......................... ..................i............................................................. ...........N.....N..............N..........................N.................... ..............i......N...........N......................N....................... ..........................................................N....N................ ..................................................N.....N................ Pos Score Pred ------------------------ 45 0.383 - 63 0.216 - 81 0.495 - 111 0.604 Yes 144 0.755 Yes 196 0.121 - 202 0.130 - 214 0.278 - 241 0.151 - 357 0.605 Yes 400 0.086 - 424 0.493 - 529 0.266 - 549 0.693 Yes 619 0.375 - 627 0.771 Yes 652 0.197 - 676 0.508 Yes 715 0.359 - 805 0.266 - 973 0.652 Yes 1012 0.129 - 1015 0.180 - 1059 0.716 Yes 1132 0.172 - 1138 0.125 - 1153 0.357 - 1180 0.199 - 1215 0.511 Yes 1222 0.497 - 1234 0.461 - 1257 0.457 - 1339 0.257 - 1344 0.122 - 1411 0.063 - 1417 0.066 -

Explain the output -- Processing locus 465: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGATTGGAATTTATTAGAAGTAAATTAACCGAACCGAATCAAATTGAATAAAAAAACAAAGAGATTGGTATGAGAAAA CCCAAACTAAACCAAGAGTGAATTTGGAATCAAAGGTTAGAGAATTCATCTTCGTTTGCTTGTTTTTGTTCTCTCTCCCA GTTCCTGGGTTCACGACGAGGAATGTTGCATATGATTTTATCTCAAAGAGTAATCCTTTTACGAAAATATCACTCTTCTG CAAATGCCCTTGTTACCAAATCACCAAATTCTATCCCCGAGTTGGTTAAACATATTGATTCGGATCTGATACGTAATGCA CATAAGGTGTTCGACGAAATTCCTGAGTTAGATGTCATTTCTGCTACAGCGGTGATTGGTCGCTTTGTGAAGGAAAGCAG ACATGTGGAAGCAAGTCAAGCCTTCAAAAGGTTGTTGTGTTTAGGCATAAGGCCAAATGAGTTCACGTTTGGCACGGTCA TTGGGTCTTCTACAACTTCGAGAGATGTCAAATTGGGTAAGCAGCTTCATTGTTATGCGTTAAAGATGGGACTTGCGTCG AATGTGTTTGTTGGTAGTGCGGTTTTGAACTGTTATGTGAAACTGAGTACGTTGACAGATGCTCGTAGATGTTTTGATGA CACTAGAGACCCAAACGTAGTTTCTATTACCAATTTGATTAGTGGGTACCTTAAGAAACATGAATTTGAAGAAGCTCTTT CGTTGTTTAGAGCAATGCCTGAGAGAAGTGTTGTTACTTGGAATGCAGTTATTGGAGGGTTTAGCCAGACGGGGCGGAAC GAAGAGGCTGTGAATACTTTTGTGGATATGTTAAGGGAAGGTGTGGTGATACCTAACGAATCAACCTTCCCTTGTGCTAT AACAGCCATATCTAATATAGCTTCTCATGGTGCTGGTAAGAGTATTCACGCCTGCGCTATAAAGTTCTTGGGGAAGCGAT TCAATGTCTTTGTTTGGAACTCTCTGATCAGTTTTTACTCAAAATGTGGGAACATGGAAGATAGTCTTCTGGCTTTCAAC AAGCTCGAAGAAGAACAACGGAACATTGTATCTTGGAACTCTATGATATGGGGTTATGCGCACAACGGAAGAGGAGAAGA AGCTGTAGCCATGTTTGAGAAAATGGTTAAAGACACAAACCTGAGACCAAACAACGTGACAATCCTTGGAGTATTATTTG CGTGTAACCATGCGGGCCTAATACAAGAAGGGTACATGTATTTTAACAAAGCTGTGAATGATTATGATGATCCAAACCTA CTAGAACTGGAGCACTATGCTTGTATGGTGGATATGCTTTCTAGGTCCGGCCGTTTCAAAGAAGCAGAAGAGCTTATTAA AAGTATGCCTCTTGACCCAGGAATCGGGTTTTGGAAGGCATTGCTTGGGGGATGCCAGATTCACTCAAACAAGCGTCTGG CTAAATTAGCGGCGTCAAAAATCTTGGAATTGGATCCTAGAGATGTTTCATCATACGTAATGCTTTCAAATGCTTATTCT GCTATGGAAAACTGGCAGAATGTGTCGCTGATACGGAGGAAAATGAAGGAGACTGGTTTAAAGCGTTTTACGGGATGTAG TTGGATTGAAGTTAGAGACCAAATTCGTGTCTTTGTGAATGCTGACAAGAACAACGAACTTAAGGATGAAGTCTATAGGA TGTTGGCACTTGTTTCTCAACATCTTGAAGAAAATGAATGTTGGAAAGACTTGTGAATACAACACATCATGTTTTTTCTC TTCTGCTGTTCTTAAAAAGGAAGAAGTAAATCGCATAGTAATTTGTATCACTGCAGCAATCAAACAGTATCAAAA .......................................................................N........ ................................................................................ ......................N........N................................................ ...N.......................................................................N.... ...............................N................................................ ..N.....................................................N....................... ........................N.............................N..........i.............. ...........................................................N.................... ..............i...........................N..................................... ...........................i.................................................... ..........................N..................................................... ...N.......................................i.........i.......................... ..........................................N....N.......N........................ ..........N...........N......................................................... .........N.........................i.....................N.....N..N............. ................N.......N........N.............................................. ....N..............................................N............................ ..........................................N................N.........N.......... ...N...............N......................i...............................N..... ......................................N..........................N.............N .................................N...N..............................N........... ........................................................................... Pos Score Pred ------------------------ 72 0.181 - 183 0.348 - 192 0.462 - 244 0.166 - 316 0.177 - 352 0.102 - 403 0.142 - 457 0.174 - 505 0.229 - 535 0.129 - 546 0.838 Yes 562 0.177 - 595 0.159 - 619 0.159 - 629 0.223 - 637 0.100 - 700 0.482 - 735 0.594 Yes 763 0.137 - 828 0.555 Yes 907 0.286 - 964 0.413 - 1004 0.645 Yes 1014 0.837 Yes 1083 0.213 - 1088 0.400 - 1096 0.390 - 1131 0.498 - 1143 0.429 - 1210 0.239 - 1236 0.527 Yes 1258 0.121 - 1264 0.370 - 1267 0.291 - 1297 0.167 - 1305 0.489 - 1314 0.494 - 1365 0.377 - 1412 0.273 - 1483 0.345 - 1500 0.147 - 1510 0.364 - 1524 0.287 - 1540 0.222 - 1563 0.744 Yes 1595 0.137 - 1639 0.250 - 1666 0.498 - 1680 0.173 - 1714 0.493 - 1718 0.248 - 1749 0.128 -

Explain the output -- Processing locus 466: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCATCATGTGCGTTTATCTAAATAAATTGGAAATATTGTTTCTTTGTGTTAGAGATCTTAACTGGTTCCGTTGCACCTTT TGCAGCTAAACCTATTAATGACTTAATTTTGCTGCAGACAGATCCGGTTTCCACCTTTTGGTACTGAGCATGTCTGCACG AATTGATATACTTCAAGTTTTGATTGATTTTCTGATTAGAGATCAATGTTTATTGATGTGTATTGCTGATCTTTTGTTAT TGGTTTTGCTTCAATTTTTAGGTGAGATACCTCATGTAGCAGATGTACCAGCTACTGTTATGACTGAAAAAGATGCTGGA GGGTTCAATATGAGCACTTTTATGAATAGGAAATTTCAGGAGCCTATTCAGCAGATCAAAACGTTCTCTTGGATGGGTTT TTCTTGGACTTGCAGGAAACGGCGTAAGCATTATCAGTCTTATCTCCGGAATGGTGTCAGAATATCTGTGAATGATTTTG TGTATGTTTTAGCGGAGCAACATAAGAGACTTGTTGCATATATAGAAGACCTTTATGAGGATTCTAAAGGCAAGAAGATG GTTGTGGTACGATGGTTTCACAAAACTGAGGAGGTTGGTTCTGTTTTATCTGATGATGATAATGACAGAGAGATATTTTT TTCTCTTAATCGGCAAGATATCAGTATTGAGTGCATAGATTACTTGGCTACTGTCCTTAGTCCTCAGCATTATGAGAAAT TTCTTAAGGTGCCGATGCATGTTCAGACAGTAGCTTTCTTCTGCCAGAAATTATATGGAGATGATGGTTTAAAGCCGTAT GACATTACACAACTAGAAGGTTACTGGAGACAAGAAATGCTTAGATACTTGAATGTATCTATTTTAAAATCATTTGAAGG TGCTCAAGCCCCTGGTACTGACCCAGGATTAAAAGCTCCATTGGTTGGTTGTGTTGGGATTAGATCAAGGAAAAGACGCC GTCCTAGTCCTGTTGGTACTTTGAATGTATCATACGCAGGTGACATGAAAGGTGACTGCAAATCTAGTCCAGATTCTGTT TTGGCTGTTACAGATGCTTCTATATTCAAGGGTGACGAAGATGGTTCTTCCCATCACATTAAAAAGGGTTCACTTATTGA AGTTCTTTCTGAAGATAGTGGCATCAGAGGTTGTTGGTTTAAGGCTCTGGTATTAAAGAAACACAAAGATAAGGTTAAGG TCCAGTACCAAGATATTCAGGATGCAGACGATGAATCTAAAAAGCTAGAGGAATGGATTTTGACATCTCGGGTTGCTGCT GGTGATCATCTGGGGGATCTTAGAATCAAAGGACGGAAAGTAGTAAGACCAATGCTGAAGCCCAGCAAAGAAAATGATGT ATGTGTCATCGGGGTTGGTATGCCTGTGGATGTGTGGTGGTGTGATGGATGGTGGGAAGGGATCGTGGTGCAGGAAGTTT CTGAAGAGAAATTTGAAGTTTACTTGCCAGGTGAGTTAAGACACCTCTGAATGCAAATTTTTATTGCAGTTGAAGATCTA TCTATATACTATTTTGTGTGACTAATTATTGTTGTTTTGGCTGTTTTCTCTCAGGCGAAAAGAAAATGT .....N.......................................................................... .................N...................................................N.......... .............................................N.........i........................ .................................N........N................N............N....... .........N...........N..................................................N....... ..................................................N....................N........ ...N..................................................N......................i.. ...........N........................................N..N.....N.................. .......................................................................N........ ..............N...N...................................N.....N..N..............N. ....................................i...............N........................... ................................................................................ ........................N...................i................................... .............N..........................N....................................... ................................................................................ .....................N........N.....................i........................... ...................................................i.....................N..i... N..................N.........N..............N...N............................... ..................................................N............................. .................................................................N... Pos Score Pred ------------------------ 6 0.305 - 98 0.337 - 150 0.359 - 206 0.470 - 216 0.626 Yes 274 0.152 - 283 0.091 - 300 0.374 - 313 0.134 - 330 0.425 - 342 0.145 - 393 0.171 - 451 0.315 - 472 0.372 - 484 0.187 - 535 0.130 - 558 0.759 Yes 572 0.385 - 613 0.085 - 616 0.110 - 622 0.102 - 712 0.187 - 735 0.455 - 739 0.173 - 775 0.386 - 781 0.283 - 784 0.250 - 799 0.131 - 837 0.664 Yes 853 0.215 - 985 0.241 - 1005 0.706 Yes 1054 0.213 - 1081 0.209 - 1222 0.432 - 1231 0.265 - 1253 0.567 Yes 1332 0.567 Yes 1354 0.382 - 1357 0.567 Yes 1361 0.260 - 1380 0.295 - 1390 0.149 - 1405 0.218 - 1409 0.481 - 1491 0.124 - 1586 0.055 -

Explain the output -- Processing locus 467: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAAGTTGAACTCTAAACGACAATGGTTGTTGCGTTTCACTGTATTAGACTCTTAAACGTTATGGGCCTTACTAATGTAA TATGGGAAGTAAATGTAATAAAGAAACGCTAAAAGATCTTGCGTTTTAGTGAAACACTGGGTTATAACTACCATATGGCC CATGGGCTTGAAAATTAAGCAAAGAAAATATCTGAGTGGCATAGACGTAATTAAAAGAGTAGCTGAGGGATATTGTAGAG AAGAAAAAAGTTGTATATTAATGTCCCCTTGGCTCGATCATCACGCCCAAAGTTTCTTCTTCACCTTGTCTCTTTTCACA GTCTCACACAGCATAGAAATAATCGAAAGATTTCTTTTTAAAATCTGGGATTTGTTTTTTTTGTTCATTGATCATTTTCG ATTGTTAGAGAAGAATCAAATGGCGATGGCTATGGATCGGATCGTCTTCTCCCCTTCATCTTACGTCTATCGTCCTTGTC AAGCTAGGGGATCCAGATCTTCCAGAGTTTCCATGGCTTCTACGATTCGCTCTGCGACTACAGAGGTTACGAATGGAAGG AAACTGTATATCCCTCCCAGAGAGGTGCATGTCCAAGTCAAACATTCAATGCCGCCACAAAAGTTAGAGATCTTTAAGTC TTTAGAAGGATGGGCTGATGAAACCCTGTTGACTTATTTAAAACCTGTTGAGAAATCGTGGCAGCCTACCGATTTTCTTC CCGAACCTGAGTCGGAAGGATTCTATGACCAAGTCAAGGAGCTAAGGGAAAGGTGCAAGGAACTTCCTGATGACTATTTT GTGGTGCTTGTTGGGGATATGATCACTGAAGAAGCACTTCCTACTTACCAGACCATGTTGAACACATTGGATGGTGTTAG GGATGAGACAGGAGCTAGTCCTACTCCTTGGGCTATATGGACCAGGGCTTGGACTGCTGAGGAGAATAGACATGGGGATC TCCTTAACAAGTATCTTTATCTGTCTGGTCGGGTAGACATGAGGCAGATTGAAAAGACTATTCAGTACCTGATTGGTTCT GGAATGGATCCAAAAACTGAAAACAACCCTTACTTGGGTTTCATCTACACATCATTTCAAGAAAGAGCGACCTTCATCTC CCATGGAAACACTGCCAGACTGGCGAAAGATCGTGGAGATTTGAAACTTGCGCAGATATGCGGGACCATTGCTGCTGATG AGAGGCGTCATGAGACTGCTTACACCAAGATTGTAGAGAAGCTCTTTGAAATTGACCCTGATGGCACGATCTTGGGCCTG GCTGATATGATGAAGAAAAAGATATCAATGCCTGCACATTTAATGTATGATGGCCAAGATGATAACCTGTTTGAGCACTT CTCAACCGTTGCCCAGAGGCTCGGTGTCTACACTGCCAAGGACTATGCTGATATTCTGGAGTTTCTTGTTGAACGGTGGA ATGTGGAGACTTTGACAGACCTTTCTAGTGAAGGACACAGGGCCCAGGACTTTGTCTGCGGACTACCTGCAAGAATCCGT AAAATTGAAGAGAGAGCTCAAGGAAGAGCCAAAGAAGCTGCCAAAAACATACCATTCAGCTGGATATTTGGTCGAAATAT CAGGGCTTAAGAAGTTTACTACACAATTCTCTTTCCAATTATCCCAAGTAAGTCATAAAGGTCTTCACCAACCATTTCAC CAATGAAGGACCAGTTTTTAAGACCACGA ......................i......................................N............N..... ....................N........................................................... ................................................................................ ...................i.....i.....N................................................ ................................i.......................................i....... ............................N...................i............................... .........N.......N.............................................................. ........................N............................................N.......... ..................i...................................i...............N......... ..N.................................N..................................N........ ......................................N......................................... ...i............................................................................ ..N......................................................N...................N.. .........N..................................................N................... ......N..i.................i..............i...N..N........N..................... ............................................N................................... N............................................................................... ................................................................................ ................................................................................ ..N.......................... Pos Score Pred ------------------------ 23 0.722 Yes 62 0.355 - 75 0.198 - 82 0.411 - 93 0.184 - 155 0.355 - 162 0.274 - 261 0.188 - 420 0.749 Yes 426 0.686 Yes 432 0.397 - 513 0.762 Yes 553 0.501 Yes 589 0.245 - 609 0.608 Yes 650 0.438 - 658 0.462 - 745 0.097 - 790 0.339 - 819 0.811 Yes 855 0.571 Yes 871 0.424 - 883 0.152 - 917 0.285 - 952 0.306 - 999 0.435 - 1044 0.801 Yes 1123 0.351 - 1178 0.430 - 1198 0.150 - 1210 0.065 - 1261 0.140 - 1287 0.465 - 1290 0.760 Yes 1308 0.655 Yes 1323 0.665 Yes 1327 0.226 - 1330 0.214 - 1339 0.197 - 1405 0.419 - 1441 0.226 - 1683 0.069 -

Explain the output -- Processing locus 468: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATAACTAATATTATTTTACTGATAAAATTCTCTATAGCCACTTGAATACTATAATCATATGTGATGCTATAAATGTAT AATTTTAATTTTATAAGTTAGCTTTACGTATTTATGATAGAGATGGTAACACTTTCCAACTTTGGCCTTTTCGATTTTGA TATATGTATGGCTCCTACGCCTTTACTTACTTTTCCCTCTCATTCATTACACTTCACGCCTCCTTTATCTCTCTTTAATA ACTCCAAAAAAAAACAAAAACAAAAAAAGAGAGAGATGGAAAAAAAACTGAAACGGCGTGAATCAATGTCAACCACCGCC GCAGAATCACCGCCGGCGAAACGAAGAAGAACAGTCACCGGAAATGAAAACTCCGCTTTAATCGAAGGACTTCCCGATCA CATCTCAGAGATCTGTCTGTCTCTCGTTCACCGTCCGTCGTTACTCTCCGCCGTGTGTACACGGTGGCGCCGTCTTCTTT ACTCGCCGGAATTTCCTTCGTTTCCGTCACTCTACGCTCTTTTCGTCGATTCAACATCGGATACGGGTCGGGTCAACCCG TCAGTCCGGTTTATGTGTTTCAACCCGGTTTCTTCAAAATGGTATCCGCTTCCTCCTCCACCACCTGACCCGCCTCTTCA CCGGATTCTTTACCGTCATCCGTCGTTTATCTCCTTTAATTTACCGATTCAATGCGTCTCCGCCGCCGGAAAACTCATTC TCATCGCCGGAAGTAACCAACAACTCTCTCCGGCGATCTCTCACCCGTTAATTTTCGATCCAATTTCGTCATCCTGGAGC TCCGGTCCTCGAATCGGATCTCCAAGACGGTGGTGCGCCACCGGAGCTTGCGACGGAGCTATCTACATCGCAAGCGGAAT TAGTTCTCAGTTCTCATCAACCGTCGCGAAATCTGTCGAGAAATTAGATCTAACCGAACAAAACCGGAATAACCACCGGT TTAATTGGGAGAAATTGAGAGATATGAGAGATTTACGGTTTAGTCGAGAAGCTATAGACGCTGTTGGATATAGAAGAAAG CTTTTAATGGTAAATGTTAAAGGAGACGCGGTTAAAGAAGGAGCTATATACGACGTCGTTAAAGATGATTGGGAACCTAT GCCGGAGGAGATGTTGGTTGGATGGAGAGGCCCGGTTGCGGCGATGGAGGAGGAGATTCTTTATTCGGTGGATGAGAGGA GAGGGACAGTGAGAAAGTACGATGATGAGAAGAGAGAGTGGAGAGAAGTGGTTGTGGTTGAAGGTGGAGAAGAGATGCTT AAAGGAGCTACGCAAGTAACGGCGGATTCCGGTAAACTTTGTGTTGTTACTGGTGATGGGAAAATTGTTGTGGTGGATGT TGCGGCGGAGCCGGCGAAGATTTGGAATGTTGAGATTCCTGATGGACTTGAACCGGTTTCGGTTCATGTTTTACCAAGAA TGAGTCAACCGAATTTTTGCTGACGTATTTTTTTTGTGATATTATTATTATTATTATTATTATTCTTTTCCTTTAGTATA CGGAACTTTTTTTATTTTCTGTTTTCCCGTTTTTCTGGTGGA ............................................................N....N........N..... .................................N........N..................................... ...N...N........................................................................ ...................................i.............................i.............. ...........................................N.................................... ................................................................................ ................................................................................ ............N.........................i......................................... ...................................................i............................ ................................................................................ ................................................................................ ................................................................................ .......................i........................................................ ......i......N..................................................N.............i. ..........i..........i.....................i...........................N........ .....................N..N.................................................i..... .......................................................N....................N... ..........................N..............N.......................N.............N ................................................................................ .......................................... Pos Score Pred ------------------------ 61 0.120 - 66 0.226 - 75 0.355 - 114 0.235 - 123 0.344 - 164 0.492 - 168 0.486 - 276 0.800 Yes 306 0.729 Yes 364 0.470 - 573 0.279 - 599 0.633 Yes 692 0.527 Yes 984 0.632 Yes 1047 0.785 Yes 1054 0.157 - 1105 0.240 - 1119 0.701 Yes 1131 0.807 Yes 1142 0.601 Yes 1164 0.582 Yes 1192 0.134 - 1222 0.150 - 1225 0.149 - 1275 0.800 Yes 1336 0.093 - 1357 0.045 - 1387 0.113 - 1402 0.178 - 1426 0.120 - 1440 0.082 -

Explain the output -- Processing locus 469: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATATAGCTCTTTGATTGTATTATTAATTATTGCTACTAAACTTAAGATTTTATTTTTGTTATTTGGAATGTAATCTGT TATTTCTTTATATAAATATAACTAATTAATATAATTATATATATATGTAATGTAATCTGTTATTTCTTTATATTGATGAA ACTTAACATAAAAAAAAACAATAAATACATTGCATTGTGTCTGAAAACCCAAAAATATTATTCGATAGCAATGTCTCTTG CAACCACAATGATCGCCATCTTTCTTCAAATCATTACATATTTTCTCATTACCACCACAGCCTCATCACCTCAGGGCTTC ACTATCGACTTGATCCACCGTCGCTCCAATGCATCTTCTTCTCGAGTCTTTAATACTCAGCTCGGATCACCTTACGCTGA TACCGTCTTTGATACCTACGAGTACCTGATGAAACTACAAATCGGTACTCCTCCTTTCGAGATCGAAGCTGTCTTAGACA CCGGAAGCGAGCACATATGGACACAATGTTTGCCTTGTGTTCACTGCTACAACCAAACCGCTCCCATATTCGACCCTTCC AAATCCTCAACCTTCAAAGAGATAAGATGCGACACACATGATCACTCTTGTCCTTACGAGCTTGTCTACGGCGGCAAAAG CTACACCAAGGGAACCTTAGTGACCGAGACAGTCACGATCCATTCCACTTCAGGGCAACCCTTTGTGATGCCTGAAACAA TTATTGGTTGTGGCCGCAACAACTCAGGGTTTAAACCGGGTTTTGCGGGCGTTGTTGGTCTAGACAGAGGACCTAAATCC CTCATCACTCAGATGGGCGGTGAGTACCCAGGTTTGATGTCTTACTGTTTTGCTGGTAAGGGAACTAGTAAGATCAATTT TGGAGCGAATGCTATTGTGGCAGGAGATGGGGTTGTATCAACTACTGTGTTCGTCAAGACCGCGAAACCAGGTTTCTATT ACCTAAACCTAGACGCGGTCAGCGTTGGGAACACCCGCATTGAGACAGTGGGGACACCCTTTCATGCCTTAAAAGGGAAC ATAGTGATAGACTCTGGAAGCACTCTCACGTACTTTCCTGAGAGCTACTGCAACCTAGTAAGAAAGGCAGTGGAACAGGT TGTGACAGCGGTTCGATTCCCGCGCAGTGACATTCTTTGCTACTATTCAAAGACCATAGATATCTTTCCGGTGATCACAA TGCATTTTTCTGGCGGTGCGGATCTTGTCTTGGATAAGTACAATATGTATGTGGCATCGAATACAGGAGGAGTTTTTTGC CTGGCTATTATATGTAATAGTCCAATAGAAGAAGCTATCTTTGGGAACAGAGCACAGAACAATTTCTTGGTGGGTTATGA TTCTTCTTCACTGCTGGTTTCTTTCAAGCCCACCAATTGTTCTGCTTTGTGGAATTGAAGTAACAAACCCTAGAGAGTCT CTTTTCCATCATTTTCAACTTCAGTTTGTTTTTCTCGGTTAACTTCAGTTTGTTATTTATATATATCTTATCAAAAT .....................................................................N.......... ............................................N....N.........................N.... ......................................................................i......... ........N....................................................................... ............................N................................................... ............................N................................................... ................i........N...................................................... ..........................i..........i.......................................... ...................................................................i............ ................................................................................ ............i.......................i........................................... ........N.................i..................................................... ...............................................................N................ ................................................................................ ...............................................................................i ............................................i...N............................... ...........N................................................................N... ................................................................................ ............................................................................. Pos Score Pred ------------------------ 70 0.170 - 125 0.463 - 130 0.271 - 156 0.258 - 231 0.774 Yes 249 0.275 - 349 0.282 - 429 0.473 - 497 0.701 Yes 506 0.348 - 587 0.808 Yes 598 0.553 Yes 708 0.596 Yes 813 0.528 Yes 837 0.611 Yes 889 0.119 - 907 0.517 Yes 1024 0.341 - 1200 0.505 Yes 1245 0.551 Yes 1249 0.148 - 1292 0.382 - 1357 0.199 -

Explain the output -- Processing locus 470: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTAAAGCTTCTCTTATTTTCCAGACGAACACTCGTGTGTGTGGTGGTGGGTGTTCCTGATCTGATCTTCCTTGACTGTA CTTGTAACCAGGGTTTGTTGAATCCTCATGTGCTCGATTCTTGCTAGGTTTCAGGTTTTGCAGCCACCACAATGGATGAG AAAGAAGAAGACCCGATTTCAGTTCCTTCTCAATCCCAACTCAAAATTCGTCTTTTTTCCTCGCGCGATTTCTTCTTCTT CTTCTTCTTCTTCCGACAACAACAACGATGGTTCAGTTTCATCTTCCAGACAAAACAACCGACAGATGGGTTATGATCCT TCAGAGGAACTCTTTGGTGTTGATTTCAAGCCCAGGTTCATATCTGGTGATTCGCGTGAACCAAGGTCATGGTTTGGTCC TAACGGTCAGTACATTCGAGAGCTTCCTTGTCCAACTTGTAGGGGAAGAGGTTATACTTCATGTTCAAATTGCGGAATCG AGAGGTCGCGATTAGACTGCCCTCAATGCAAAGGAAAGGGCATAATGACTTGTCTCAGATGTTTGGGGGATTGTGTCATA TGGGAAGAATCAATCGATGAACGACCTTGGGAGAAAGCTAGATCCAGTTCTCCATTTAGAGTAAAGGAGGATGATGAGGT TGATAATTTAGAGATAAAGTTCAGTAAAAGGCGAAAATCGAAGCGGATTTATCAGTCACCAACTCCTGAAGTTGGACAAA AGATCAGCCGATCTCTAAAAAGTCTCAATGCCAAAACTGGACTTTTTAGTAAGCGCATGAAGATTATACATCGTGATCCA GTGCTTCATGCTCAAAGAGTAGCTGCAATTAAGAAAGCTAAAGGAACACCAGCTGCTAGAAAGCATGCATCCGAATCTAT GAAAGCTTTCTTCAGTAACCCTGTAAACCGTGAACAGAGAAGCCTATCTATGAAAGGAACTAAGTTTTACTGCAAGAACT GTGGACAGGAAGGCCATAGACGCCATTACTGTCCAGAACTAGGCACTAATGCGGACAGAAAATTTAGATGTCGGGGTTGC GGCGGGAAAGGCCACAACCGTAGAACTTGTCCTAAGTCGAAATCAATAGTCACTAAAGGCATTTCCACTAGGTATCACAA ATGTGGAATATGCGGTGAGAGAGGTCACAACAGCAGAACATGCCGGAAGCCGACCGGAGTGAATCCTAGTTGCAGTGGTG AAAATTCCGGGGAAGATGGTGTTGGTAAAATAACGTATGCTTGTGGGTTCTGCAAGAAAATGGGACATAATGTAAGAACC TGTCCAAGTAAACAAGTTTCAGACAGTGATTCTTGTTTAGAGCAAGAAGGTTCTTGAACCTGTTTATTTGGTTAGGCTTG GTTTTGCTATTGAAGCTGAAATAACCAATCACTGACAAGTCCTTTTTAGCGGATGGATATTTCGGGTTTATAATCG ................................................................................ ...........................N...........................................i...N.... ................................................................................ ...........................N.....................................i......i....... ....................................................................N........... ............................................................N................... .........................N..................i.............N....................N ................N.....................................................N..N...... ................................................................................ ...........................N............................i....................... .......N........................................................i.............i. .................................................N.............................. ................................................i..................N............ ................................................................................ N........N.............................N........................................ ...............N....................N......................i.........N.......... ................................................................................ ....................................................N....................... Pos Score Pred ------------------------ 108 0.168 - 152 0.549 Yes 156 0.356 - 268 0.382 - 306 0.820 Yes 313 0.811 Yes 389 0.480 - 461 0.118 - 506 0.316 - 525 0.542 Yes 539 0.241 - 560 0.326 - 577 0.393 - 631 0.316 - 634 0.340 - 748 0.201 - 777 0.626 Yes 808 0.080 - 865 0.512 Yes 879 0.677 Yes 930 0.398 - 1009 0.531 Yes 1028 0.394 - 1121 0.417 - 1130 0.386 - 1160 0.182 - 1216 0.139 - 1237 0.211 - 1260 0.652 Yes 1270 0.284 - 1413 0.036 -

Explain the output -- Processing locus 471: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 472: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCCAATTGGTTGGTATAGTTGACTAATGACTATATATTTTTTATTCTAGCTGAACATCAATATGACTTATCATATATGT GATCCAAAGTCATGGTCTTAGTTTATGTACTGTTGCAATATTCCTTCCTATCTACATATATTACATCATGAAAACAAGGT AAACTTATCATCTAAATTTTGTAGTTCATTCTTCAGAAGAAACTTCATGGGTTTCCACACAAAATCGCCGGACCGAGTAA CTCATGAAGAAGAAGAAGCAAATATAGGAGTTGATAATCAACCAAGAGAAACGACATCAACATCTCTTAATAGGTCACAA ATTATTAAGACAAGAAACTGGTGGATTTGTATCTTTGTCTGCTCGTGTTTGGTCGTAGCAGGACGTGTTTTATCCACCCT TCTTTTAAACTTCTACTTCATCCAAACAGGACGAGATGTTTGTGATGACCCAAAGCAATTTAAAGGCACATGGCTCCAGT CTATGGTCCAAAACGCTGCGTTTCCTTTTACAGCCTTTCTTCTTCTCTTATGGCGTTCTTCATTTTCCACCCATAGTGAA ACTTCCTCTTCTTCTTCTTCTTTTGGCAAACTCTTTCTTCTTTACATCTCTCTCGGCGTCCTATTTGCGGCTTATAGCCA ACTCTATGCAATTGGGCGAACGCATTGTGTGTTCTTCTTGTGGATCTTTACTTCCCAATTGATTTTCACTTCCATATTTA CAACAATTATCAACAAACAAAAATTCAACCGTTGGATCATATTATCCATGGTCCTTTCTGGAGCTGCCACAGGCTTAGGC ATTACATCTTCAGGTGGCGCCTATATTCCCTGCGAAAATGAAGGCTCAAAAATGAGTAACGGCGCTTGGTGTGCCTTCTT TGGCACGGTAGCATTCTCTTTATCTTTATGTATCATGCAATTAGGGTTCCAAAAGGTCATACCGACTACACAGAGTAGAG TTTCCGCGGTGATTCTAATGCAAACCAATGCTTCTATGATTGCAACGTTGATATGTTTGGTTGGATTATTCGTTAGTGGT GAATTCAAAGATATAAAAGAAGATTTTGAGACGTTTAAGAAGGGGAAGCCACTTTATGTCTTGAGTCTAATTGGATTATC GCTTGCGTGGCAAGTAATGTCTTTAGGGCTTGTGGGGCTAGTGTGTTTGGCTTCTTCTCTTTTCTCTAATGTTGTCAGCT TCTGCTCAACGCCATTAGTAAACATTCTCCTTGTGTTGGCGTTTCGGTTTACGGATGCCGACGTTAAATTTTTTAAGGAA GGAGCTCTGGTAGCTGGGATTCTTGGTTTTGCATCTTATGTGTATTCCTTGTATAAGTCTACCAAGAAAAAGGAAATAGC AAGCCAAAGCCAAACAACTAGAGTATGATTATATTATATTGATCATATGAATTTATGTGTGTGTAATTTCTGTTGTTATA CACTTTCATGTGATTCATACTGTTACTCAGTGTTTGTCCATTTCACT ..........................N...................................N.............N... ...........N............N..........................................N............ ..............................................i................................. ...N............................................................................ ................................................................................ ...................................N........N........................i.......... ..N..............................................N.............................. ................................................................................ .....N.......................................................................... ...............................................i................................ .....................................i.............i............................ ...........................N......N............................................. .................i.........N.......N................N........................... .......................................................N........................ ................N...................................................N........... ......................................................N......................... .....................................N.......................................... ........................N.....................N.......N......................... .......N....................................... Pos Score Pred ------------------------ 27 0.263 - 63 0.299 - 77 0.191 - 92 0.239 - 105 0.184 - 148 0.359 - 207 0.661 Yes 244 0.420 - 436 0.367 - 445 0.145 - 470 0.611 Yes 483 0.175 - 530 0.311 - 646 0.336 - 768 0.630 Yes 838 0.511 Yes 852 0.760 Yes 908 0.317 - 915 0.280 - 978 0.575 Yes 988 0.169 - 996 0.281 - 1013 0.332 - 1096 0.154 - 1137 0.149 - 1189 0.220 - 1255 0.320 - 1318 0.151 - 1385 0.199 - 1407 0.078 - 1415 0.074 - 1448 0.045 -

Explain the output -- Processing locus 473: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCGAAGACGAGGTTACAAAAAAAAAAGAAAAAAGAATTCTGGTTAGAGTTGAGAAAAAATAGTAAGTTATGTAGCATTC CACTTCTATAAATTCCAATGAATACTGTCTAAAAGTTTTGCTAAGAACTGTATAGCCATGATGAGAATGACCGGTTTCAT AGTAGCTATGATGATAGCAGCGGCGATATTCACTACTTGCGTTGATGGAAACAAGTTCTTTTATCACCGGGAAATTAAAG TACTTCGACATTTGAAACGGTTCAACAAGCCTGCTCTTAAATCCATTAAGAGTGAAGATGGAGATGTAATAGATTGTGTT CCTATCACTAATCAACCAGCTTTTGATCATCATTTACTTAAAAACCATACCATCCAGATGAGACCGAGTTTCTACCCGGT AAGTGATTCTACGTACACCAAGAGGGAGGCAAAGGCTGTAACTCAGGTTTGGCACAAGGCTGGAGAGTGTCCCAAAAACA CTGTTCCAATCAGAAGAACAAAGAAAGAAGATCTCTTGAGACCAAAATCCATCAGGAGTTTTGGGAGAAAGTCTCATCAA AGCATTCCAAGAACTACAACCTTTGATCCAACATTAGGTCATCAGTACGCGCTAATGGGTGTGAGAAATGGAAAATTTTA TGGGACAGAAGTTGCAATAAATCTGTGGAAACCATATGTCCAAATTCCTAAAGAGTTTAGCTTGGCTCAGACTTGGGTTG TGTCCGGAAATGGCTCTAGTCTAAACACCATTGAAGCTGGTTGGCAGGTTTATCCAGAACTATATGATGATAATAATCCT AGATTCTTTGTTTATTGGACGCGTGATGGGTACCGAAAAACGGGATGCTACAACCTTCTCTGCTCAGGTTTTGTTCAAAC GAGTAATCGATACACCGTAGGTGGATCAATCACCACCATGTCACGCTACCGTGGAACTCAATATGATCTCTCCGTACTTA TATGGAAGGACCAAAAAACAGGAAACTGGTGGCTTAGAGTCAATGAAAAAGACGTTATTGGGTACTGGCCAGGCTCGTTG TTCAATTCTCTAGGAAGAGAAGCTACGAGAGTTGAATGGGGAGGTGAAATCATTAACTCAAAGACCGGTGGGAGACACAC TACCACAGATATGGGAAGTGGGCATTTTGCAGATGAAGGTTTTAAGAAAGCAAGCTATTTCAGGAATCTTAAGATAGTTG ATGGAACCAATACTCTTAGAGAACCACAAGGACTTTACTTCTTTGCTGATAAACATAACTGTTACAATGTTAAGACAGGA AATGGTGGAACTTCTTGGGGGGCTCATTTCTTCTATGGTGGTCCTGGTCGAAACGTTAAATGTCCTTGATTAGGATCTGT GTGTTCAAAAGCTTTTATAATCTCACATACACATGTCTATATTTGAATAAGAACATTTGCGAAAACCACGTCATCAGAGA TGTTTATA .....................................................................N.......... .................N.......................................N..N.....i............. .......N..N.................................N................................... .........................................................N.....N................ .........................................................i...................... ................................................................................ ................................................................................ ......................................................i............N...........N ...................................N............................................ .........N.....................................................N..N............. .........................N..................N................................... .....................................N........................i................. ...................................N............................................ ..........i.....................N............................................... N.................................................................i............. ................................N..............................................N ........ Pos Score Pred ------------------------ 70 0.224 - 98 0.291 - 138 0.332 - 141 0.467 - 147 0.558 Yes 168 0.365 - 171 0.413 - 205 0.299 - 298 0.437 - 304 0.186 - 378 0.620 Yes 615 0.720 Yes 628 0.334 - 640 0.128 - 676 0.257 - 730 0.410 - 784 0.227 - 787 0.295 - 826 0.438 - 845 0.149 - 918 0.336 - 943 0.576 Yes 962 0.278 - 1003 0.432 - 1076 0.305 - 1131 0.554 Yes 1153 0.167 - 1201 0.187 - 1267 0.592 Yes 1282 0.455 - 1315 0.224 - 1340 0.294 - 1393 0.052 - 1440 0.055 -

Explain the output -- Processing locus 474: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGTTTTCACCATTTTCGGAGATTATCACCTTCTCCCCGATTCGCCGCCTTGTGATTACATCTACGTAAAGAGTTTCTGG TAGAAATTTTCCCTCTTTTAGCTGCAGATTGGCATCAGATTCCGTTCTGGATGTGTCGGTGATCGATTTTCCGCGTCGGT GATGATAGCTAACGGATTCGAGAGTATGGATAAAGAACGAGTCAGGAAACGTCCTCGTATGACCTGGGACGAGGCCCCAG CTGAACCAGAGGCTAAAAGGGCTGTGATTAAAGGACATGGAAGCGATGGGAGGATTTTGTCACCACCATTAAGGGACGAT GATCGTGATGGTCATTATGTTTTCAGTTTGAGGGACAATCTCACTCCTAGATATAAAATACTTAGCAAGATGGGTGAAGG CACATTTGGTCGGGTTTTGGAATGTTGGGATCGTGATACAAAAGAATATGTGGCAATAAAGATAATTCGAAGCATTAAGA AATACCGGGATGCAGCAATGATTGAAATTGATGTACTTCAGAAACTTGTTAAGAGTGATAAAGGCCGCACACGCTGTGTA CAGATGAAGAACTGGTTTGATTACCGAAATCATATTTGCATTGTGTTCGAGAAGCTTGGGCCAAGTTTGTTTGACTTTCT AAAGAGAAATAAATACTCCGCATTTCCTCTGGCCCTTGTTCGGGATTTTGGATGCCAGCTTTTGGAATCTGTAGCATATA TGCACGAGTTACAGTTAGTTCACACCGACCTCAAGCCTGAGAACATTCTGTTGGTGTCTTCTGAAAATGTAAAGCTTCCT GACAATAAGAGGAGTGCTGCAAATGAGACACATTTCAGGTGCTTACCAAAGTCAAGCGCCATTAAACTGATTGATTTTGG CAGTACTGTTTGTGATAACCGGATTCATCACTCCATTGTCCAAACAAGACATTACAGATCCCCCGAGGTCATTTTAGGTC TAGGATGGAGTTACCAGTGTGACTTATGGAGTATAGGTTGTATACTATTTGAACTATGCACGGGTGAGGCTCTCTTTCAG ACGCATGATAACCTAGAACATCTAGCTATGATGGAGAGGGCGCTTGGACCTTTACCTGAACATATGACTCGAAAAGCCAG CCGGGGTGCTGAGAAATATTTCAGGAGAGGGTGTCGGTTGAATTGGCCAGAAGGAGCCAACTCTAGAGAAAGTATTAGGG CTGTAAAAAGACTTGATCGTCTCAAGGATATGGTATCGAAACATGTTGACAACACGAGATCTAGATTTGCAGATCTATTG TATGGTTTATTGGCATATGATCCATCTGAGCGTCTCACAGCAAACGAAGCTCTTGACCATCCTTTCTTTAAGAGCTCAAG TTGAGAAGAAGCAAAATCAGAACCATAAAATGTAGAATTTTACTTGGTAACAAGTTTTTTTGTTAGATTCCTCTAAAGCT CCCGTTCTCAGTAGCACCAAACA ................................................................................ ..................................................N............................. ....................................N........N................................N. .......N........N....................................................i.......... .....................N.........................N................................ .........N.......N............N................................................. ...N............................................................................ ...................................................N...........................i ..................................................................N............. ......................N......................................................... ................................................................................ ....N....................i.............................i........................ ....N......................i..i................................N................ ................................................................................ .............................N............N..................................... .............................N.................................................. ....................... Pos Score Pred ------------------------ 131 0.339 - 162 0.772 Yes 186 0.587 Yes 219 0.595 Yes 277 0.271 - 286 0.228 - 319 0.338 - 328 0.298 - 337 0.346 - 390 0.612 Yes 422 0.289 - 448 0.340 - 490 0.355 - 498 0.426 - 511 0.127 - 564 0.201 - 692 0.431 - 720 0.685 Yes 787 0.241 - 823 0.231 - 965 0.339 - 986 0.653 Yes 1016 0.646 Yes 1045 0.152 - 1068 0.533 Yes 1071 0.639 Yes 1104 0.415 - 1230 0.468 - 1243 0.326 - 1282 0.494 - 1297 0.266 - 1390 0.236 -

Explain the output -- Processing locus 475: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACCAATATTTTACAAAATATTTTGATATACATATAATTTACAAGATAATACCCAGACTGGCTTATTGTGTAACTTAATA ACATTTGAGGCGAATCTATGAATAAAGAGAAATAAAGGACCTATTCACAGATTCAGACAACATCGTCGCATTTTGATTTT GAATCGATTATTTTACCTACACTCGTGTATGAGCCAGTCCTCCGGAATAAGACAACATCTCCAGGAGTCTCTACGAAATT CTTCGGCTCTCAAAGGTATTCACGACGGTCTTTTAGCTCTATTCTCTATTGAGAACCTGATTTTTCCGGTGAATTTTGAT TATTGCAAAGTTGGAGAATTTTTGAGATTTAGAAGAGATACTGGCTTAGGATTCGTATTCATGTCGAGCTTATTACACTG TAGAAATGTCGTAGATTGTGCGTTTACTTCTTCCACTGTTCGTCGCATTGTCAATTTAGGGTTTTTAGTGGGTAGAATAA GAACGTTGTGTTCAGTGTCACAAGTGAAATCAGAGAATCTCGAAAAGCAAAAACCATGTTTGGAATCTTTTACAGTTTCT TATCTCGTGGATTCTTGTGGACTGTCTTTGGAATCTGCTAAATCGAATTCCAGATTCGTCAAATTGGTTTCGTCCAAGAA ACCAGACTCTGTTCTTGCTCTCTTCAAGGACCATGGCTTCACCAATGACCAGATCACCAGTGTCATCAAATCCTTTCCTC GTGTTCTGTCACTGAGCCCGGAAGATGTAATCTCGCCCAAGCTCATGTTTTTCAGTTCTATCGGGTTTTCGACCTCGGAT ACTGCGAAAATGATCTCTTCTTCCCCGAAAATGCTATCATATAGCTTGCACAAAAGATTGATCCCTTGCTATGATTCCCT CAAGAGCATACTCGTTGAGGAAGAAAGTGTTGTCAAGTGTCTCAAGCGTGGAATCAGATGTTTCTCATTGAAGATTACAC ATTGTGTGTCTCTTCGTGTCTCCATTTGTAGAGAGCTTGGAGTTCCTGACAAAAGCATCAAGTGGTTGGTTCAAGCGTCT CCATTCACTTTTTTCTCTCGTGAGAGAAGATTCAACGAGGTCTTAAACAGAGTTTGCAGCTACGGTTTTGACCCGAAAAA GGCGGGTTTTGTTCACGCTATGGTAGCCTTTGATTGCACGAGTGAATCAGCAATGGAGCGGAAATTTAAGTTGTTCCAAT GTTTTGGTTGGTCTAAAGAAGATTTCGTCGCAGCGATCATGAGATTCCCAAACTGTGTGACGGTTTCAGATGAGAAGATA ATGTACACATTGGAGTACCTTGTAAACAACATTGGTCTGCAAGCTCGTGACATTGTTGCAAGACCGGTGGTGTTATCTCT GAGCATGGAGAAGAGAATCAAACCGAGGAACCAGGTCATCTCTTTGCTTCTCTCCAAAGGACTTGTGAAGAAGGAAGACA TCAACTATTTCACTATACTAAAGCTGAAAAGCTCCGAGTTCATGGATAAGTTTGTGCTGAAATATCAGGATGAGATGCCA CAACTTGTACAACCCTTTACAAGTAATCGATGAGTTTGTTCTATTAGAGAAGTGAGAACACACTCTTGGTTGCGGCTGAA CCTTGCCGACTCCGATCATGTTTTGATGAATTTTGTGACATTTAATTTGACA ................................................................................ .................N.............................................................. ............................N................................................... ................................................................................ ............................................................N................... .....i.......................................................................... .......................................................i........................ ................................................................................ ................................N...........N................................... ........................N...................N................................... .........N....................N.......................................N......... .........................................................N...................... ................................................................................ ................................................................................ ...................N................................i.........................N. ......................................i..............................N.......... i............................................................................... ....i........................................................................... .........................................N...........................N....N..... .............................N.................................................. .................N.......N.......................... Pos Score Pred ------------------------ 98 0.420 - 189 0.118 - 381 0.369 - 406 0.656 Yes 536 0.587 Yes 673 0.273 - 685 0.270 - 745 0.452 - 765 0.137 - 810 0.409 - 831 0.411 - 871 0.441 - 938 0.136 - 1140 0.323 - 1173 0.714 Yes 1199 0.351 - 1239 0.573 Yes 1270 0.276 - 1281 0.727 Yes 1365 0.517 Yes 1482 0.397 - 1510 0.352 - 1515 0.441 - 1550 0.278 - 1618 0.053 - 1626 0.049 -

Explain the output -- Processing locus 476: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAGCAACAGTATCATAAAATTCAAAAGTTCAAAATATATTTACGGACAGAGTATGCTGCGACAAAACTCAATTAACTTG GGGTATCCCCCGGTTTTGTGACTTGATTTAAGATTTCGATTTGGTTTGATTTTCGGTTTGGTTCAAATGTGGAACTTAAA AGGTGACTCTCTCTCTGTGTCAGAAGTGTCTTCCTTTCCTCTGCATTCTCTTCTTCTTTGTGCCGTGTGGGTGTGGCATT TCTCTTGTAGCGACAGGAATTGTGCCAGAGCTGCAACAGCAATGGCGACGGAATCAATTTTTCTCAAGCCTGTATCTTTC GCATGGTTTACGAAAAACCCATTGAATCAATTTCATAAAAGAAGACAACTTTCCTATCGAATTCGTCGTTTTCGATTCGG TGTAATTTGCTCCAAGTCATCTGATTATCAAGATTTCCAGAGTTATGCAAGACCTTTGCGTCTCTTACCAGCAGAAGAAG TGAAAGTTTTTAGAGGGAATCCATCTTTCACTGTGAGCGAATCTCGATCTCTTTATAAGGTTAAGCTACAGACAAGTAAT TTGTTTGGTTCAGGGATTAGTGATATGAATGCTAGAGTTCTTATATGCTTGATAGACGAAAAGGGCGATTCGGTATTACA GACAATACCTCCAAATTTGTCATCTTTGGATCCAGAGGTTGTGGAAAATGGTGAAAGCTTCAGGTTTCAGAGGGGCTCTC TTGATGAATTCTCATTTGAAGGACCGAAACTCGGCAAAATCAGAGCTTTTTGGATTGGTCTTGAGTCTGGTCAATGGAGA GTTGGAAGAGTGAGCTTGTGGGTGGTTAATCCTCGTGGTAAAGAAACCGAGGCAAAAACCTTTTGTTACCGATATGATTT TGAAGTTGATGAACTCTTGCTTGGAGAGAGCAGTGACCTATCAATGGTGGAACTTAGACCTAGCCGTATCACTGAACTCG CTGACTCCGATCAAATCTCTTCGTCTTCAGCTCTGAATCTTGACCGTACTATGGTTTCCAATGAGGAAAGCATGGAAGAA TACGCAAACTTGAAACTCTCTCTGCTACTCTATGATGCGGTTCTCATCCTTCTTGGAAGTTTGGTCTTTTCCTTCTCACT TGGTGAAAACTCCGCCATTGCTTTCTTCTTCGGTGGAACTATGGGATTTCTCTACTTATTGCTATTACAGAGATCAGTTG ATGAACTGCAAGTACCGGGATCTTCTTCTTCCGAAAACTCCAATCAGATACTCAGCGGGGGACTCAAGATTCCGGTTTTG AGCCTTGCATTGGCCATAGGATTAACGGTTTTGGCCATGAGAGGACACATTGGAGGAGAGATGACTGCATTTGCAGTCAC ACCAAAAGAGATAGTGGTGGGGACTTTGGGGTTTCTTGTATGCAAAGTAGCCGTTATTTTGGCTGCCTTTAAGCCCTTGA AGGATGGTCCTTAAATCTTAAGAGAACCAATGTTCTGATGCTGTACATGACTTTGTTGTATATAACAGATATGTAAATAA GAATAGATACATCATGTGATCATACATGTATAT .....................................................N.......................... ..................................................................i............. ................................................................................ .........................................i...................................... ..N............................................................................. ............................................N................................... ................................................................................ ........................i...N...............N................................... ...............................................i................................ ...N.....................................................................N...... .........................................................................N...... ........N..................................i.................................... ..................................................N.........N..........i........ ...............................N..N............................................. ........................................N....................................... N............................................................................... ....................................N.......................N................... .......................................N........................................ ...N.........................N.......N........N.......................N......... .............N...........N....... Pos Score Pred ------------------------ 54 0.212 - 147 0.615 Yes 282 0.584 Yes 323 0.464 - 445 0.120 - 585 0.559 Yes 589 0.112 - 605 0.416 - 688 0.501 Yes 724 0.363 - 794 0.350 - 874 0.274 - 889 0.126 - 924 0.668 Yes 1011 0.466 - 1021 0.171 - 1032 0.762 Yes 1072 0.126 - 1075 0.347 - 1161 0.477 - 1201 0.478 - 1317 0.328 - 1341 0.444 - 1400 0.186 - 1444 0.315 - 1470 0.255 - 1478 0.095 - 1487 0.048 - 1511 0.040 - 1534 0.070 - 1546 0.043 -

Explain the output -- Processing locus 477: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGGTTACTGCTTTTATTAGATTCTAAAGCTAGGGTTTTTGGGAACGTGCTTAATCTGTCTGTATCTTAGCATTTGAGA TTGGGTTTTTGGGTATAATAACCTATTATGGATTCAGAATTGAGTTTTTCTTCGAGGGATGTGGTGAATTGCTGTGAATA TGGTTGTGATTATTCTCTTGGGGCATGTTCTTCAGATCCTTTGGCTAGGACGGTGAAAAGAAAGTTCAACGAATTCAAAG AAGGGAACAACATGATGTTGTTACGAGGTTCTTCTGATTCATCTTCTTCAAACGCTAAGATACTAGTTGAGAATGAATGT GCAGCTTTACTTGAGGCTCTTTCTAGCCAAAGGGAAACAGTTAAAGATCTTCATTTAGAGCTTGAAGAGGAGAGAAACGC TGCTGCATCTGCAGCTAATGAGACTATGTCGATGATACTGAGGTTGCAGAGAGAAAAGGCTGAGATTCAGATGGAAGCTC GGCAATTCAAAATGTTTGCTCAAGAGAAAATGACACATGACCAAGAAAAGCTCTCGGTTTTGGAGAATTTGTTGTATGAG AAAGAACAAGCTATCGAGGCATTGACTTATGAGGTCGAAGCATACAAGCATAGATTGTTGAGCTATGGGGTATCTGAAGC TGAGATACATGATCAGATACTTGGTTTTGGCAGAGACTCTAGCACGGTTGGCTTTGATGTTTACCCTTGCGAGTATACTT CTTTGGAATGTAGTGTGGATGAGAACCCGAGTGGACCAGATGGTAACTTTGAGATTGAAGAAAAGGTGATGGTTGGTCAG TCTCCAAGATGGCCATATTATGATCCCAATTCGCCTTTAGGAACTGCAAAAGATATTAAGGGGGCATCTTTCGCAGACTC TCCTATGTCTAGTAGTAGCGACCGAGTTTATACTATCGACTCCATTCACGTGGGAGTTTCTGAAGTTAAGATTGACGATG AGCCTAATAAGATGAGTAAGGGAAAGTTAAATGGTGATCACTGGAACTCTCCAAGATACCAAGAACCGTTCACGACTCAG CAGGGAGTTAACGAGCCAGATATAGAGAAGCTTTACACAAGGCTTCAAGCACTTGAGGCAGACAGGGAATCACTGAGACA GATCATTGTGTCGATGCGGACAGACAAAGCTCAGTTGGTGTTGCTGAAAGAAATCGCACAGCATTTAACAAAGGAAACCG GCGCAACAAGCAGGCGAAACCCTGTTAGCAAAATGCCATCTCTTAAAGTATTCTCTGTAGTAACGGTCTTCAAGTGGATT GTGTCTTTCGTTTCGTGGAAAAGGAAAGCCAGACAAAACAAGTATGTTTATGAGTTGTCAGCAAATAATATGGGGATGCT TATGATTCTAGGCGAGGGATCTGGAACTAGGAGATGGAGATGTTTAACGAGTTCACATGTCTAGATTTCCATTTTTTTAT CAGGCTTCTTTGGCGTAGTTAGTGCAAAACAAATGTACATTGCTCTGCACTCCAATCAAATATGTATGTTGGATGATTCA GAA ................................................................................ ...........................i..............................N....................N ........................N....................................................... ...........i..i.........................................................N...N... ................................................................................ .................N.......i.....N......................................N......... ...........i.................i......N......................................N.... ............................N...................................N............... ........N...............................................N....................... .......N..........N....................N............................i........... ........N..........N............................................................ ....i........................................................................i.. ...........i..................i................................................. ................................................................................ .............N.................................................................. ................................i............................................... ...........................................N.....N...................N.....N.... ................................N............................N...N......N....... ... Pos Score Pred ------------------------ 108 0.723 Yes 139 0.369 - 160 0.330 - 185 0.112 - 252 0.565 Yes 255 0.698 Yes 313 0.226 - 317 0.469 - 418 0.053 - 426 0.523 Yes 432 0.174 - 471 0.464 - 492 0.657 Yes 510 0.664 Yes 517 0.241 - 556 0.145 - 589 0.128 - 625 0.210 - 649 0.422 - 697 0.220 - 728 0.340 - 739 0.095 - 760 0.185 - 789 0.726 Yes 809 0.400 - 820 0.115 - 885 0.790 Yes 958 0.540 Yes 972 0.802 Yes 991 0.590 Yes 1134 0.478 - 1233 0.661 Yes 1324 0.415 - 1330 0.314 - 1350 0.171 - 1356 0.212 - 1362 0.098 - 1394 0.475 - 1400 0.216 - 1417 0.120 - 1473 0.118 - 1502 0.089 - 1506 0.043 - 1513 0.029 -

Explain the output -- Processing locus 478: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTACGTTCTTTATCTCTCTTTCTTCTACCAAAATTTTCAAGAAATCAATTGGTTTATGTTAGATATGACTAAATGGGTTT TGTTTTGACTCTTTTTTCTAAATCAAAATCGTGAAAATAGAAGCACACATGTTTTGGTGGCATTATAAAAGTCCATATAG AGTTGAAAATCCTTCCAAGCCATGGCCTATCATCCTCTGGCTCCAAGGTGGACCTGTAAGTTTTTATCTTCTTTTATTTT ACATCTTATTCGTCTTTTTACACATTTGTTGTTTATTGATCATTAATGTTTTCAATTATTTGGTTTTGATGAAACAGGGA GCTTCAGGGGTTGGTATAGGGAATTTTCAGGAGGTTGGACCATTAGACACATTTCTCAAGCCCAGAAACTCAACTTGGTT GAAGAAAGCCGATCTTTTGTTTGTGGATAGTCCAGTTGGGGCAGGGTACAGTTTCGTGGAGGGCAATCAAAAGGATTTGT ATGTGAAAAGTGATGAAGAAGCAGCACAAGATTTGACCAAACTGTTGCAACAACTCTTCAACAAAAACCAGACTTTAAAC CAAAGCCCTCTCTTCATTGTTGCTGAATCTTATGGTGGCAAAATCGCGGTTAAGCTCGGTTTATCGGTTATTGACGCAGT TCAATCTGGCAAATTGAAGCTTCATCTTGGAGGAGTGATTTTGGGAGATAGTTGGATATCCCCTGAAGATTTTGTGTTTT CATGGGGACCTCTTCTCAAGCATGTCTCTAGGCTTGATGACAATGGCTTGGATAGCTCTAATAGCTTAGCAGAGAAGATT AAAACACAAATTAAAAATGGTGAATACGTTGGAGCGACGCAAACATGGATGGACCTCGAAAACTTGATTAGTTCCAAATC CAATTTCGTCGATTTTTACAATTTTCTATTGGATACTGGTATGGACCCTGTCTCGCTCACAACGAGTCTAAAAATCAAAA AGGAAGAGAAAATAAAAAAGTATTCAAGATATTTAAATGATATGAGAAGTTTAAGTGACGTAGAAGATGTTGAAGGAGAC CTTGATAAATTAATGAATGGCGTTATTAAGAAGAAGCTCAAGATTATTCCCAACGACCTCATATGGGGAAACAATTCGGA TGATGTGTTTACAGCAATGGAGGCTGCTTTTATGAAACCCGTAATTGAAGACGTCGATGAGCTTCTTGCCACGGGGGTAG ATGTGACCATCTATAATGGACAACTTGATGTTATCTGTTCAACAAGTGGAACTGAAGCATGGGTTCACAAACTCAGGTGG GAGGGGCTAGAAGAATTCAAGAAAATGGAAAGAGAGCCATTATTCTGCGAAAGTGACAGAGCAACAAGAGGCTTTACCAA ATCATACAAAAATCTCCATTTTTATTGGATTCTTGGTGCTGGTCATTTTGTACCAGTAGATGAGCCATGTGTAGCATTAA AAATGGTTGGAGAGATAACCAAGTCACCACAACTTTGACCCATTCTTTCTTCCAAACTCTCACATCACAATATGACTCCT TTCACATCTGATTGATTTTATTACTCAAACGTTAAAAATAATAATAAAAGAGTCTAC .......................................................N........N.......i....... ................................................N............................... .....................N.......................................................... .............................................N......................N........... ................................................................................ ................................................................................ N...........N................................................................... ...............................N................................................ ................................................................................ ................i...........................i...N............................... ........................................N....................................... ....................................N....i........................N............. ............N...N.............................................N................N ..N.............i..............N........................N....................... N..............i...........N..............................i..................... ........................i....................................................... ...........................................................N......N............. ..i....................................................................N........ ......................................................... Pos Score Pred ------------------------ 56 0.291 - 65 0.244 - 73 0.512 Yes 129 0.301 - 182 0.196 - 286 0.382 - 309 0.366 - 481 0.112 - 493 0.119 - 592 0.139 - 722 0.201 - 742 0.318 - 757 0.227 - 763 0.837 Yes 817 0.641 Yes 845 0.702 Yes 849 0.469 - 921 0.452 - 997 0.225 - 1002 0.564 Yes 1027 0.334 - 1053 0.409 - 1057 0.222 - 1103 0.230 - 1120 0.143 - 1123 0.183 - 1137 0.816 Yes 1152 0.288 - 1177 0.142 - 1201 0.131 - 1216 0.562 Yes 1228 0.278 - 1259 0.824 Yes 1305 0.584 Yes 1420 0.174 - 1427 0.365 - 1443 0.531 Yes 1512 0.119 -

Explain the output -- Processing locus 479: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTTAATTCATTTATTTTATTTTATTAATGATTGCTTTCGGTGGTAAAGCCCATAGTAAGAACATATTAATGGGCTGCTA ATATATCTAGGCCCATTATAGATGGAGATAACGCCGTTAGTTGGAAGTCCGTTAGGACCGTTAGTTACATCAAGCAAAAA CCCTATCGAAGAAATTGCAGTGTGTTTTCTTACAGCGAAGAGCCCCAAAGAGAAGAACAAAATTTCGAAAATAGAGAAAG AGAAGAAGTTGACGATGGATTCAAAGGCGAAGCAGATGATTCTTTCAGCTCTCTGCAAACACTTCTCTTTGGATCCTGAA CCTTTTGTGGGCGGAGCGGGTGAAAGTGATATCAAGACACTTTATTCTAATGTATTGAAGGCATCTGGAAAGGAAGTATC TGCACAAAACAACGATGTTTTGAAGTGGCTAGATTTTGCGGAAGGTTTCTCTTCAGATTCAAAGGATTGGTTTTCAGCTC TTGAAAAGTTGAATTTAGAGTTAGCTACCAAGTCAGTGCTCTTGGGAAACGGATTGACGCCATCGGCAGCTGATGTTGCT GTGTTTTCAGCTCTGCATTCTTCAGTGCTTGGCCTCTCAGATTCAGACAAGGAGAAAGTACCACATGTCATAAGATGGGT GAATTACATCCAGAATAAAGAAGAGTTGTCGACATTATTTGCACCAATACCTGTGAAGCTACCAGAATTTAGCTTCGAGG TACCAAAACCTGCCATTAAGGTGGAAACAAATTCTAATTCAAAAAAAGCTGCAGAAGGTGTAAAGCCTGTTGACAAGCCA GATGTGCAGCCGCAGCTGGGCACAAAGAAAACTGAGCCAGAGGAACCAAAGAAAAATGCAGCAAAAGAGAAGGATGCCAA AAAGGAAAAGAAGAAGCCTGCTGAACCAGAACCAGCTAAGAAAGAGGCAGAGCTTAGTGTCAGTTTGCTTAACATTCAGG TTGGTCTAATTCGTAAAGCATGGAAGCACCCATCGGCGGATAGTTTGCTGGTGGAAGAAATAGATGTCGGGGAGGATAAA GTGAGACAAGTAGTTAGTGGCTTAGCAAAGTTCTGCAGTCCTGAGGATCTAACGAACCGCCTCGTTGCACTCATCACAAA TGTCAAACCTGGAAAGCTAAGAGATGTAATGTCACAAGGACTGGTTCTTTGTGCTTCAAGTGAAGATCACTCGGTGGTAG AGCCGTTGCTACCACCTGCTGGTGCTAAACCAGGAGAGCGTGTTTCATTTTCAGGGATTGAAGGAAAGCCAGAAGATGTA CTGAACCCAAAAAAGAAGCAACTAGAGAAGATAACACCGGGTCTCTACACTGATGAAAATGGCGTAGCCACATACAAAGG CATACAATTCATGACCTCGGCTGGACCTTGCACTTCCAGCATCCCCAAGGCGACCATCAAGTAGTAAACTCAGTTGATAC TTCTCTCCTCTACTCTAGCGTTTTTCTTTGATATTCGCAAACTCAGTTGGAAAAAGAAAAGATGTTCTTTTTGTCACAAT TCG ...........................i.........................................i.......... .....................i.......................................................... ................................................................................ ..............i....................N............................................ .................................................N.............................. ..............N................................................................. ........................................................................N....... ................................................................N.........i..... ................................................................................ ................................................................................ ................................................................................ ...................i...........................................N................ ...............................................................................N .......................N....i................................................... ...........................................................................i.... ....................................................N.....i..................... ..........N..................................................................... .............................................................N.................. ... Pos Score Pred ------------------------ 28 0.665 Yes 70 0.529 Yes 102 0.639 Yes 255 0.755 Yes 276 0.211 - 370 0.114 - 415 0.384 - 553 0.096 - 625 0.313 - 635 0.724 Yes 802 0.202 - 856 0.253 - 874 0.163 - 980 0.602 Yes 1024 0.339 - 1120 0.356 - 1144 0.411 - 1149 0.702 Yes 1276 0.518 Yes 1333 0.128 - 1339 0.502 Yes 1371 0.448 - 1502 0.142 -

Explain the output -- Processing locus 480: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATAGGGCTCTTTCTTTTTTAATTTCTCTTAGTTCCCTTTTAATTATAAAGTTAATTAGTTTAATCACACGAACGAGCTA GGAGCCGCTTCTGATATATAATCTATAAAACCTAGAATTGTATGTGTCGAGTCATTAAACCTTAGGCCCCCTACGAAAAA CAAATCTTCAAGTATGTGCAACAGATTCATGGAGAAAGAAGGAACCCTGGAAAGAGACTATGGCCGAAACCCCACCGGAA AACCCTCACCGGGCGACTTTCATTCTGACACATACAACCTTTACTTTAAGGGTTTGGTAAGGGAGAAAACATCAGCTCAA TTATCGGCGGGATTTGAGGTTGCAATTTTCAGAGAAGAGGATGAATATCTCTTGTTTCAGATGAAAGGATCACTTCATGA CTCCACAGTTACAGTTTTGGAGGCTGAGCTTATGGCATTGAAGCGAGGATTAACCGAAGCCGTGAGTTTGGGAATCAATC ATATCTCAATCTGCTGTGATCGTTTAGAGTTATATGAATTGGTCATGGGCAGATTAGCGCCTGAGAAAGAGAACATCGCC TTGCTAATGGATGATGTGCAATGCATAAGGCGAGAACTGACATCTAGCATTCCTGTTACTGTGACTGAAAATCAAGCCAA GTTTGCGTTTAAGCTTGCAAAGGAATCAATAAGCATACGTACGCCTCCTACCGAGCAGAAGGCTTGCGGTGAGGTTAATA TCGAACATGAACTGATGTTTTCTGTTGCTTTATGCCGTCATCAATTCGGTGTAGAGTGGATGAAACAACATATAGAAGTG AGACTAATCGAGGGAGATGTTCCGAGATGTCCTCATGATGGCTGCAAATCAATCCTATCTCTTAAAAGCTGTTCCCATCT TTTGACACCTAAACTAGAAGAGATGTGGGAACATAGGATCAAAGAGGAGTTCATCCCTGTATGTGACAGATTTCATTGCC CAAATCCAAGGTGTTGGGCTTTGATGTCGAAAACCGAGCTCGTTGAGTCTACTGAAGATGGAGTTAGGAGATGCTGCTTT AAATGCCGCAAAGCCTTTTGCATTAACTGCAAAGTTCTGTGGCATAGTGACTTGTCATGCAAGGAATACAAGACGTTGGG TCGAAATCCTAAAACAATATCGCGTCAATGTAAAAAGTGCCAACACATGATCAAACTGTCGCATAAAACCATCAATGTAT ATTGCAGGTGTGGTTATTCATTTTGCTACACATGTGGAGCTCAATGGAAGCTTGGAGGTTGCCGTCATCATAACCAAATG GTGGCGGCTGTGTTGGTTCTCGTTTTTTTCTTTATTTTTCATTCAATGATTATGTGGTCAATTAGTCGTCGTGTTTAATC CAAACACATAGATTAATAGGTGTTTTGAATTCTCTTTTCCATATCTTTCTTATTATATTGCTTTGTTAAATTTGACAATT TTAAGATTTAATTTCTG ................................................................................ .........................................i...................................... .............i..............N..............................i.................... ................................................................................ ........................................N...................N...............N... ...............................N................................................ .................................N..........N................................... ......i...N..N......i........................................................... ................................................................................ ......N.......N................N...........................N.................... ................i.........N.......i..N.......................................... ......................i.....................................N................... .......................N.................................i............N......... ..N.....................................................N....................... ...........................N..................N...........................i..... ...............................N...........N.................................N.. .............................................N.....N............................ ................................................................................ ................. Pos Score Pred ------------------------ 122 0.521 Yes 174 0.665 Yes 189 0.241 - 220 0.724 Yes 361 0.249 - 381 0.376 - 397 0.254 - 432 0.372 - 514 0.246 - 525 0.433 - 567 0.617 Yes 571 0.123 - 574 0.421 - 581 0.711 Yes 727 0.214 - 735 0.412 - 752 0.099 - 780 0.351 - 817 0.549 Yes 827 0.428 - 835 0.587 Yes 838 0.422 - 903 0.660 Yes 941 0.124 - 984 0.268 - 1018 0.691 Yes 1031 0.240 - 1043 0.206 - 1097 0.302 - 1148 0.228 - 1167 0.266 - 1195 0.611 Yes 1232 0.288 - 1244 0.283 - 1278 0.264 - 1326 0.092 - 1332 0.120 -

Explain the output -- Processing locus 481: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGGCTTCAACTGAAACACATCTGTGAAGCTAATGGGCTTGTATACAATAACCAGTGGGCTTTTCATAAACAAGTTTAGT GAGCTTTTCAATGAATAGTAAGGGCTTATTGTAAGTAAAACCCTAATTTCTATACACTCTTCTACGCTATCTTCCATTCT TCCTCCAGCTATCATATCAAAAACAATGAGAAAGAAAATGATGATTTCCGATCTTCCACACGATTTGGAATCGGAAATAC TCTCTAGGGTTCCGGCGAAGTCCCTAGCAAAATGGAAGACTACTTGCAAACGATGGTATGCTTTATTCAGAGATCCGAGT TTCGTCAAGAAGAACTTTGATAAAGCAGGAGGAAGGGAAATGATAGTACTGATGAATTCTAGGGTTTATTCAAACAGCGT CAATCTCCAAGGGATTAACAATAGATTTGATCCAAGTATGGAGGTTACAGGTAAACTCATCAAGCTAAATGATTCAAAAG GTGTCGATATTTCTGCTATCTTTCACTGCGACGGTTTGATATTATGCACTACGACGGAAAGCACTGGACTTGTGGTTTGG AACCCGTGTACCGGTGAAATTAGATGTATCAAACCCAGAATATTTTACCGGTGTAATGATAGGTATGCTCTTGGTTACGG AAACAGCAAATCTTCTTGCCATAGCTACAAAATCTTGAGGTCTTGCTGTTATTACGTCGACCAGAACCTCAGCCTCATGG CTGCTGAGTTTGAGATCTATGACTTTAGCACTGATTCTTGGAGAGATCTTGGTGACATCACTCGTGACATGATCGTATAT TCCAGTGGCGTGTCTTTGAAAGGAAATACTTACTGGGTTTCTGGATCTAAAGAAAAAGGTTTTTTCATGCGGTATTTTGA TTTTAGCAAAGAGGTATTTGGGCGTCTCCCTCTTCCATATCAGAGTTTTAATGCTAACCATACCGCGGCTCTATCAGCTG TTGGGAACGAAAAAATCGCCGTGTTGCAACAAAAAATTCTTGCAATGTCAGATGAGATGAGGATTTGGGTCACCAATAAG ATTGATGAGGCCAAAGACTTGTCGTGGAGCAACTTTCTCTTAACAGTGGATTATGGTAAATTTAATTTACCATGTCTAGT GAATGTGACAAGCTTCTTGTTGGACGAGGAGAATAAAGTGGCAGTGTGTTCTGACGTAGACACGAAAGATGGACTTAGGA GCAGAATCTACATTGTGGGAAAGGATTTCTATAAAGAAGTTTTTAAAGATACAAGGGGATCAGATAACAATTGGCCACTT CTCCTCTGTTATGTTCCTAGTTTGGTTAGCATTCAGGAAAATATACCCAACAAGGCAGAAGAAAATGAAATCAAAGGAGG TTAGTCAATTATTTTCTATTTAGTTTAGAAACACGAAGGAACTGGATGAAGTTTTTCACATGTCCTTCTTTTACTTTTGA AATTGGACGGTTGGTTTATTGAT ................................N............................................... ..........N..................................................................... .........................i...........i..N....................................... ...............................i....................i....N...................... .......................................N...........N............................ .....................................i..............................N........... ...........................................N.................................... .......................N...............................i........i............... ............................................................................i... ..................N.................................................N........... ..................................................................i............. ..................................................N............................. ............................................N......N....N....................... ....N...............................................N..................i........ ..N.................................................................N........... ................................................................................ ..........N.....................................................N............... .............................................N.............N.................... ....................... Pos Score Pred ------------------------ 33 0.462 - 91 0.191 - 186 0.740 Yes 198 0.593 Yes 201 0.381 - 272 0.594 Yes 293 0.565 Yes 298 0.411 - 360 0.320 - 372 0.384 - 438 0.783 Yes 469 0.220 - 524 0.329 - 584 0.241 - 616 0.617 Yes 625 0.547 Yes 717 0.856 Yes 739 0.130 - 789 0.373 - 867 0.758 Yes 931 0.244 - 1005 0.435 - 1012 0.125 - 1017 0.225 - 1045 0.163 - 1093 0.204 - 1112 0.587 Yes 1123 0.200 - 1189 0.112 - 1291 0.098 - 1345 0.214 - 1406 0.067 - 1420 0.051 -

Explain the output -- Processing locus 482: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCAATATTTTGAAATTTGACAAAACACTCGAATAAACTATTTAAGAAAATATACTACTGTACATATCAAATTTTATTCT TCAGATCTAAGGTTTCGTTGATATGATAATATAGAAAATAACAAGAGAAAAATGTTAATCAACAAAAAACAATCCATTTC ACAAATACGCAAATACTATATATACCTTACACTAAACCGTTTCAAATCCCACACACCCATCACCACTTTTCAACTTCTCC CTCTATCTCTCACTTTCAAAGCAATGCATTCTTCACTTCTCATAGTTCTCCTCTTTCTCCTCTCGGTCTCATCTTCCTCA GCTCAAACCTACAATATCCTCTCTTACGGAGCCAAACCCGACGGCAAAACCGACTCGACCAAGGCCTTTACCGTCCTGTG GGCCAAAGCATGTGCATCGGTCAAACCGGTCACCATTTTGGTACCTAAGGGACGGTTTTTGTTAAGAAGCATTATTTTTG ACGGTTCCAAGTGTAAACGCAAGTCCGTCACGTTCCGTATCCAAGGTACACTTGTGGCTCCATCGGATTATCGAGTCATC GGTAAAGAAAACTATTGGATTTTGTTCCAGCATCTCGACGGCATCTCTGTCTACGGCGGAGTTCTTGACGCCCAGGGAGC TTCTCTATGGTCTTGCAAAAAGTCCGGCAAGAATTGCCCGAGTGGCGCCACGAGCATAGGGTTTCAGAGCTCACGCAACG TGGTGATCTCCGGGCTGACGTCACTTAACAGTCAGATGTTTCACGTCGCGATTAACGGCTGCAGTAACGTAAAGCTCGAC GGAGTCAAAGTTTCTGCAGACGGAAACAGTCCAAACACTGATGGGATCCACGTGCAGTCATCATCTACCGTCTCCATCCT CAACTCCAAAATCTCCACCGGCGATGATTGTGTCTCCATCGGTCCCGGCACTAACGGCCTCTGGATCGAGAATGTCGCTT GTGGCCCTGGCCATGGGATCAGCATTGGAAGCTTGGGAAAAGAAAGTGTGGAGGTAGGTGTACAAAACATAACAGTGAAA ACGGCGACGTTTACAGGAACAGAGAATGGGGTGAGAATAAAGTCATGGGCCAGGCCCAGTAATGGATTTGCTAAGAATAT CCGTTTCCAGCATTGTGTTATGAACAACGTACAAAACCCAATCGTCATTGACCAAAACTACTGCCCTGGCAACGAAAATT GCCCTAATCAGGTTTCTGGGATAAAAATCAGCGATGTAATGTTCTTCGATATACACGGGACATCGGCGACGGAAGTTGGA GTGAAATTAGACTGTAGCTCTAAGAAGCCGTGCACGGGAATTCGAATTCAAGATGTGAAGTTGACGTACCGGAATAAACC AGCAACAACAGATTGTAGTCACGCTGGAGGTAGTGAGGCAGGATTTCAACGGCCCAACAGTTGTCTATGACCAGAAAAGA AAAATATTTGTCTTCTTTTTGTTAGTAGTGTAATTGTAAGTAACGTCGTATTTTCATATATATTACGGCTTTATTCCTGG AGGGTTGGA ................................................................................ ......................N............................i............................ ................................................................................ .......................i........................................................ ................................................................................ .........i...................................................................... ................................................................................ ................................................................................ ......i......................................................................... ...................................i............................................ ........................................N....................................... .......................i...............................................N........ ............N................................................................... .........................N..................N................i.................. ...................i............................................................ .................................N....N......................................... ....................................................i........................... ..................................................................N............. ................................................................................ ......... Pos Score Pred ------------------------ 103 0.111 - 132 0.523 Yes 264 0.676 Yes 410 0.600 Yes 647 0.540 Yes 756 0.676 Yes 841 0.403 - 904 0.664 Yes 952 0.170 - 973 0.148 - 1066 0.387 - 1085 0.483 - 1102 0.583 Yes 1140 0.698 Yes 1234 0.429 - 1239 0.372 - 1333 0.511 Yes 1427 0.179 -

Explain the output -- Processing locus 483: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGAATAATTACTCTTCGATTTGTGATTTCTATCTAGATCTGGTGTTAGTTTCTAGTTTGTGCGATCGAATTTGTCGATT AATCTGAGTTTTTCTGATTAACAGATGCAGATCTTTGTTAAGACTCTCACCGGAAAGACAATCACCCTCGAGGTGGAAAG CTCCGACACCATCGACAACGTTAAGGCCAAGATCCAGGATAAGGAGGGCATTCCTCCGGATCAGCAGAGGCTTATTTTCG CCGGCAAGCAGCTAGAGGATGGCCGTACGTTGGCTGATTACAATATCCAGAAGGAATCCACCCTCCACTTGGTCCTCAGG CTCCGTGGTGGTATGCAGATTTTCGTTAAAACCCTAACGGGAAAGACGATTACTCTTGAGGTGGAGAGTTCTGACACCAT CGACAACGTCAAGGCCAAGATCCAAGACAAAGAGGGTATTCCTCCGGACCAGCAGAGGCTGATCTTCGCCGGAAAGCAGT TGGAGGATGGCAGAACTCTTGCTGACTACAATATCCAGAAGGAGTCCACCCTTCATCTTGTTCTCAGGCTCCGTGGTGGT ATGCAGATTTTCGTTAAGACGTTGACTGGGAAAACTATCACTTTGGAGGTGGAGAGTTCTGACACCATTGATAACGTGAA AGCCAAGATCCAAGACAAAGAGGGTATTCCTCCGGACCAGCAGAGATTGATCTTCGCCGGAAAACAACTTGAAGATGGCA GAACTTTGGCCGACTACAACATTCAGAAGGAGTCCACACTCCACTTGGTCTTGCGTCTGCGTGGAGGTATGCAGATCTTC GTGAAGACTCTCACCGGAAAGACCATCACTTTGGAGGTGGAGAGTTCTGACACCATTGATAACGTGAAAGCCAAGATCCA GGACAAAGAGGGTATCCCACCGGACCAGCAGAGATTGATCTTCGCCGGAAAGCAACTTGAAGATGGAAGAACTTTGGCTG ACTACAACATTCAGAAGGAGTCCACACTTCACTTGGTCTTGCGTCTGCGTGGAGGTATGCAGATCTTCGTGAAGACTCTC ACCGGAAAGACTATCACTTTGGAGGTAGAGAGCTCTGACACCATTGACAACGTGAAGGCCAAGATCCAGGATAAGGAAGG AATCCCTCCGGACCAGCAGAGGTTGATCTTTGCCGGAAAACAATTGGAGGATGGTCGTACTTTGGCGGATTACAACATCC AGAAGGAGTCGACCCTTCACTTGGTGTTGCGTCTGCGTGGAGGTATGCAGATCTTCGTCAAGACTTTGACCGGAAAGACC ATCACCCTTGAAGTGGAAAGCTCCGACACCATTGACAACGTCAAGGCCAAGATCCAGGACAAGGAGGTATTCCTCCGGAC CAGCAGCGTCTCATCTTCGCTGGAAAGCAGCTTGAGGATGGACGTACTTTGGCCGACTACAACATCCAGAAGGAGTCTAC TCTTCACTTGGTCCTGCGTCTTCGTGGTGGTTTCTAAATCTCGTCTCTGTTATGCTTAAGAAGTTCAATGTTTCGTTTCA TGTAAAACTTTGGTGGTTTGTGTTTTGGGGCCTTGTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCT ................................................................................ ........................i....................................................... ................................................................................ ..................N............................................................. ............i................................................................... ................................................................................ ......N......................................................................... N............................................................................... ..........................................................................N..... ....................................................................i........... ................................................................................ ..............................................................N................. ........................................................i....................... ................................................................................ ..................................................N............................. ............................................i................................... ................................................................................ .....................................N.......................................... ...................................................N...............N...........N ..............................................N.................N............. Pos Score Pred ------------------------ 105 0.718 Yes 259 0.265 - 333 0.543 Yes 487 0.126 - 561 0.471 - 715 0.355 - 789 0.549 Yes 943 0.257 - 1017 0.537 Yes 1171 0.192 - 1245 0.680 Yes 1398 0.255 - 1492 0.188 - 1508 0.046 - 1520 0.052 - 1567 0.062 - 1585 0.064 -

Explain the output -- Processing locus 484: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTTCTGTATATCAGTGTCTCGCATTTTAAACCCTAAAGAAATTACATATTGAGTTCCTACTTCCTAACATCATATTAAA ACATTAGTTAAGAAGACTTAAATAAAAAAATCAACGCATAGTTTTGACCAACCAAAAGTCAACATATTTTTGGAACCTAC AAAATGGGCAATAACAATGTTCACACAATTCATAGAACAAACTATCATGTATGTAACAAGGCGTTTATCAGAGTATCGGA AAAACCCGTCGGAACTTAAGAAGCCTCCACCGGAAGGTCCAAACTCCGGCGTACTTGTGATTCAAGACGAAGAATCGTTG ACCACTTGTTGCTTTGGTTTGTGCAACGAGATTTTCCTAAGAGGATTACCGTTTCCACAGAACGCAGCGTTGACAGTTAG ACGCGACGACGGTGGACAGAACAATACTGTTCATCGTGATCCGGTTGTGTTCATACCTGTTCTTGGTCAACCTCTATCTT CTAACCGTTATTACACATATGAACGATGCGGGTATTATTCAAGAGAATCATCTACTAGTACTGAAAAAGAGGTGGAGAGA GTTACATGCTGCTTCTGCATTAGATTTAACCATGTTCATATGTATAAGAAGCCACAACCAGATCCTTATGACATTCACCA ACAAGTAGTGATCACAACAACCTCTTCGCCTTCCCGTTGTTACTACTTTGCGAAGCCTGTTGCTCCTAATGCTCTACTAC CTGCATTTCTTATGAGACAAGGCTGGACAATAGAAAACTCGACTTCTACACTACAAGACTTTGGTCTCATAGATGACGCA AAAGGTCTTAACTTAGAGCTTCGTTCTAAGCTTCCCGGTCTTGATATAAGCGTTGTTGTAGGGAAATGGTATGTTCCTTT CATATTTGTGAAGGAAGGAGACATAATAGATCAAGTGAAAATATCAATGTATTACAACATGACGCTTCATCAAAGATGGG AAGAAGTTTTCTTCTATGAGAATGTTCACAATGAAGATTGTGTTGAGGTCGTTGTTGATGTTGATCTTGAACTCCAAGTG ATTAACATTGAAGGACAGAAGGTTAGTATAGAGACAACGAATGTGGATGCTAAAGGGATTGTTTGGTTCCAAGTTAAAGA CAAGAAGATAGGTTTAAGGTCAGTGGTTGTGGAGAGGATGGAAAGTGAAGAGGAGAGTTTCGGGTGGAAGAAGGTAGACG GAAACCGAGTGACGGTTAAGAGATTGGATCGGTTCGAAGGCGGTTCATCGCATTGGAAGAGTTATAAATGTTATGTATTG GTAGAGAGATTTGAATTGAAAAGAATGGATGAGAGTTTGGTGTTGACATATGAGTTTAGACATGCTGATAAGTTGAGGAC TATCCAATATTGACTTGTGAGCTTCTCTCTTTTATTTAGTCTTCTGCTTTTACATTGATGTAAACTCTGTTTAAAATGAA AATAAAAATGTCATCTTTCAGAGTTGTTGGAG ................................................................................ ................................................................................ ...i............N.............................i...N............................. ................................................................................ ................................................................................ ................................................................................ ..................N......N...................................................... .....N.........................N.......N...........................N............ ....................................................................N........... ...........N............................................................N....... .................................................................i....N......... ..............................................i...........i................N.... ...............N.....N........N..........................N...................... ........................................i.....N................................. .....................................N.......................................... ...................................................................N....N....... ........................N...N....................N...........N.................. .........................................................N.................N.... .......N........................ Pos Score Pred ------------------------ 164 0.704 Yes 177 0.384 - 207 0.715 Yes 211 0.310 - 499 0.429 - 506 0.271 - 566 0.182 - 592 0.311 - 600 0.377 - 628 0.089 - 709 0.124 - 732 0.456 - 793 0.461 - 866 0.637 Yes 871 0.084 - 927 0.706 Yes 939 0.716 Yes 956 0.401 - 976 0.156 - 982 0.123 - 991 0.246 - 1018 0.062 - 1081 0.559 Yes 1087 0.212 - 1158 0.425 - 1268 0.409 - 1273 0.153 - 1305 0.229 - 1309 0.151 - 1330 0.431 - 1342 0.256 - 1418 0.037 - 1436 0.065 - 1448 0.095 -

Explain the output -- Processing locus 485: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGTTTACTAGAGTTTAAACATTCCAACATAATTCCACTAAAACTCAACCAAAAAGGGGGTACAAAAAAAGAGAAAAAAA AAATTAAATGTTATTGAGTGATGGGTAGTTAAAGAACTTATGGTGTTAGGCTGTCCAAAAAGAGAAGCAGTTAAAGAGTT TGGAGTACAATGTCTTAAACAAGAAAATGTCTGTCCCATTATAACATCTCGAAACCAAACACACACACACACACATATAA CAATCTCTTCTTCTCTGACATTACACAATGGTGGTTTGAGTTTACAGAAGATAATAATGGGGAAAACTAGCAAATGGTTT CGAAGTCTCTTAACCGGAAAGAAAGAGAGAACAAAGGAACATATCATTCAAAGCGAGTGTGTATTCACTTCTTCCATTCC AGGCACTCCTAAGGAGAAACGTCGATGGAGTTTTCGACGTTCTTCAGCAACTGGACCACCGCCACCAGCTTGTGCTATTA CACTGAAAGATTCACCACCACCGCCGCCACCACCACCTCCACCTCCACCGCTACAACAACCATTTGTGGTCGAAATCGTG GACAATGAAGATGAACAAATCAAGAACGTTTCAGCTGAAGAGATTGAAGAATTTGCAGCAATAAAGATTCAAGCGTGCTA TCGTTCTCATTTGGCGAGGAAAGCATTAAGAGCATTAAAAGGGTTAGTGAAATTACAAGCACTAGTGAGAGGTCATTTAG TAAGGAAACAAGCAACAGCTACACTTAGATGTATGCAAGCATTAATAACACTTCAAGCCAAAGCTCGAGAACAGAGGATT CGTATGATCGGAGGAGATTCAACAAATCCAAGAACATCGATTCACAAAACCAGGATCAATAACTTTTACCACGAGAATGA AGAAAACATCAAGATTGTGGAAATGGACATTCAATCGAAAATGTACTCTCCGGCTCCTTCGGCTTTAACCGAGATGAGTC CTCGCGCGTATAGTAGCCATTTCGAGGATTGTAACTCATTCAACACAGCTCAAAGTAGTCCACAATGTTTCAGCAGATTC AAAGAGTATTACAATGGAGATACTTTGTCTTCTTATGATTACCCTCTCTTCCCTAATTACATGGCTAATACTCAGTCTTC TAAAGCCAAGGCTCGGTCTCAAAGTGCGCCGAAGCAGAGACCTCCTGAGATCTATGAGAAGCAGATGAGTGGGAGGAGAA GATCTTCGATGGAAGCACCGAGGAATAACGGTGTCCCGAGAGCTGTAAGGATGCAGAGATCTTCATCTCAACTAGGGTCA AACACAGCCAAGGAAAGTCAACAACATCATCATCATCAGTACTATCCGTGGATGGCGATAAAGCTCGATAGATCTAATAT TTCGCTTATGGAGAGCGAATGCGGATCTACAAGTACCGTTATGACTAATACCAACTACGGTAGACATGTTGATGTTCAGG GAAACAACAACATGTACTGAACACTGTGCTTTAGAATTTGAGAGATTGCTGGAAAAATAGGCAAGCCTAAAAAACGAAGA GGACAGGTTTATAGGAGTTTTTTTTATGATGATGGAGAT ................................................................................ .......N............N..................N........................................ .........N................N..................................................... ...........................i............................i................i...... ................................................................................ ........................i....................................................... ................................................................................ ....i.....N..................................................................... ................................................................................ ............................N...N............................................... ...N........................................................................N... ......................i.................i................................N...... ................................................................N............... .............i....................N.........................i................... .....................................................N..........N............... ........N.........................................i............................. ...................................................i............................ .......i..........N.....................N........................N.....N........ ...........N.................................................................... .........................N..N..N....... Pos Score Pred ------------------------ 88 0.189 - 101 0.310 - 120 0.339 - 170 0.276 - 187 0.452 - 268 0.740 Yes 297 0.570 Yes 314 0.586 Yes 425 0.882 Yes 565 0.626 Yes 571 0.296 - 749 0.400 - 753 0.312 - 804 0.311 - 877 0.338 - 903 0.662 Yes 921 0.634 Yes 954 0.269 - 1025 0.189 - 1054 0.607 Yes 1075 0.179 - 1101 0.545 Yes 1174 0.140 - 1185 0.354 - 1209 0.400 - 1251 0.562 Yes 1332 0.525 Yes 1368 0.541 Yes 1379 0.093 - 1401 0.310 - 1426 0.426 - 1432 0.397 - 1452 0.449 - 1546 0.045 - 1549 0.047 - 1552 0.048 -

Explain the output -- Processing locus 486: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAACCACGATGAAAAATACATTATTTGTATCTAATATATACAGAGGATGGTCGATTCTTGATTAACGAATCTCATGTCT CATCTTCCTCTTTCACTTTGAGCAATTATGGCAGCAATGAACATCGTTGACTTTCAAAACTGCTTCTTTTTTGTCCTCTT AAGCCTCTTCTCACTTCTCTGTTACTCTCTCTTCTTCAGGAAACCAAAGGAACCTCGACTTCACTATGATTTACCTCCGA GCCCTCCGACTCTTCCCATCATTGGTCATCTTCACCTTCTTCTCTCTGTTCTAGTCCACAGATCTTTACAGACACTCTCC ACCAAGTATGGGTCTATTCTCTATCTTCGCGTCTTCAGTTTCCCTGTAGCCCTCGTCTCCTCAGCATCTATTGCTTATGA GATCTTCAGGGAACACGACGTGAACATCTCGTCTCGTGGTTTCCCTCCTACCGATGATTCCCTTTTTGCTGGATCTTTCA GCTTCACCTCTGCTCCCTATGGAGATTACTGGAAGTTCATGAAGAAGCTCCTGGTCACGAACTTGCTTGGACCCCAGGCA CTCGAGCGGTCACGAGGATTCCGTGCAGATGAGCTAGATCTGTTTTACGAGAACCTGTTGGATAAAGCGATGAAGAAGGA GAGCGTGGATATCTGTGTGGAGGCGTTGAAGCTCAGTAACAACAGCATCTGCAAGATGATCATGGGGAGGAGTTGTTCAG AGGAGAATGGTGAGGCAGAGAGAGTTAGGGCTTTGGCTACCCAGTTAGATGGCTTGACGAAGAAGATCTTATTGGCGAAC ATGTTGCGTGCGGGGTTTAAAAAGCTTGTTGTCTCACTGTTTAGAAAGGAAATGATGGATGTTTCCAGCAGATTTGATGA GCTGCTCGAAAGGATCCTTGTGGAACATGAAGATAAACTGGACATGCATCATCAAGGTACAGACTTAGTGGACGCGTTGT TGGCAGCTTGTCGGGACAAAAATGCAGAGTATAAGATCTCTAGGAACCATATCAAGTCGTTTTTCGCGGATCTCTTATTT GCAAGCACTGACACCTTTGTGCAAACAACACAGTGGACAGTGGCGGAGATCATTAATAACCCTAATGTTCTTGAGAGATT GAGAGGAGAAATCGATTCCGTGGTCGGGAAAGCAAGGTTGATTCAAGAAACTGATTTGCCAAACCTCCCTTATTTGCAAG CGGTGGTCAAGGAAGGGCTAAGACTGCATCCGCCGGGGCCTCTCTTTGCAAGGTTCTCCCAAGAAGGGTGTAGGATCGGA GGGTTCTATGTACCGGAGAAAACAACATTAATGATTAATGCTTATGCTGTGATGAGAGATTCTGATTCTTGGGAAGATCC TGATGAGTTTAAACCAGAGAGGTTTCTAGCTTCGTCAAGATCAGAGCAGGAAAAGGAGAGAAGAGAGCAAGCAATTAAGT ACATTGCTTTCGGAAGCGGACGAAGAAGCTGTCCTGGAGAAAATCTAGCATATATCTTTTTAGGAACTGCAATTGGAGTG ATGGTACAGGGATTTGAGTGGAGAATCAAAGAAGAGAAAGTTAACATGGAAGAGGCTAATGTAGGACTGAGCTTGACCAT GGCTTATCCTCTTAAGGTCACTCCTGTTCCTCGAACCCTAGTCCCCTTAACTCAGAATCCACTGAACCGGAGTTCATGAA TCTTTGGTAAGTAATTAGGTAGCCTTGTTTTTACGTTACCGTGTCTGAGTTTACTGGTTTAGTTTGGCTAATGTGTGAAA CCGTAAACAATTGTGTTG .........N.....................................N..........................N..... ...........................N........N........................................... .................................................................N.............. ................................................................................ .......i....................................................................N... .....................................................N.......................... ..................N...................N......................................... ............................N........................................i.......... .......................................................N.....N.................. ......N.........................................N............................... N..................................................i..i...N.................N... ..........................N................N.................................... .....................N.......................................................... ................................................................N............... ................................................................................ ................................................................................ .......N......................i......i.....N.......i............................ ..N............................................................................. ................................................................................ N............................................i............N...................N. ...........................................................................N.... ......................................................................N......... .................. Pos Score Pred ------------------------ 10 0.205 - 48 0.348 - 75 0.202 - 108 0.324 - 117 0.278 - 226 0.319 - 328 0.610 Yes 397 0.145 - 454 0.413 - 499 0.275 - 519 0.432 - 589 0.348 - 630 0.525 Yes 696 0.325 - 702 0.288 - 727 0.305 - 769 0.137 - 801 0.195 - 852 0.610 Yes 855 0.845 Yes 859 0.394 - 877 0.252 - 907 0.430 - 924 0.372 - 982 0.397 - 1105 0.305 - 1288 0.177 - 1311 0.652 Yes 1318 0.549 Yes 1324 0.493 - 1332 0.511 Yes 1363 0.293 - 1521 0.297 - 1566 0.850 Yes 1579 0.308 - 1599 0.336 - 1676 0.222 - 1751 0.093 -

Explain the output -- Processing locus 487: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTAAATGGAGAGAATAGTAGAAGACAAATAGAGCATAGATAAAAAGCAATAGCATACAAATTGTTGTCACGTATATGA CGTAATTGTATTAGTTTATAGCATACACCGAAAAATATCACTTTCTCTAACATAATCTCATTTTCATCTCCAATAAAACT ATTTCAAACCCAAGAATATCAAGAAACAAAGTTTCTCTCGAACGCTCCGATGTATCAAATCTTCTTTTTCTTTCTTCCTC TCCTCCACAGTTATGCCTCTGCCCAGACTCCTCCTCCGTTCAGAAACGGTGACCTCGTCGCAAATTTTGAGCCGAGTTTA GCCGTCGTCACCGGAGTTCTCGCCATCATGTTCGCACTCACTTTCGTCCTCCTCGTCTACGCTAAGTGCTGCCACATCGA TCTCCGATCAGGTTCCGGCGACAGAAGAAGGCACGATCGCCGGCTCAGACAAGGAATATTCTTTAACCGGTCAACGGCAT CATCGGATCGATTCTCTGGTTTAGACAAAACAGCAATCGAGTCTCTTCCTCTGTTTAGATTCTCTGCTTTGAAAGGATCA AAACAAGGTCTTGATTGTTCTGTCTGTTTGTCTAAATTCGAAAGTGTTGAGATTCTTAGATTGTTGCCTAAGTGTAGACA CGCTTTCCACATCGGATGTATCGATCAGTGGCTTGAGCAACACGCAACTTGTCCTCTTTGCAGAGATAGAGTCTCAATGG AAGAAGATTCCTCTGTTCTCACTAACGGAAACAGTTTCAGGTTCTTGAATCAGTCTGAGATTAGAGAAGATTCGAGCTTG GAGCTTTACATCGAGAGAGAAGAAGAAGAAGAAAGAATACACAGAGAAGAACTTAGTGGGTCGTCGAGATTCAGCATCGG AGAGAGTTTCCGGAAGATTCTGAAATTAGGTAATAAAGAAAAAACGTTACTTGATGAACACGTGAACGATAAAGATGAGA AGAAGCTTATGCACAAGTTCAATCATAGGATCGTTGTGTCGGATGTTGTTTTCAAGAATCGTTGGAGCAACGTGAGTTCA TCAGATTTGATGTTTTTGAATTCCGAGATGGTTAATTCGATTTCTAGCGAGAGATTTTCTTCATTGGATCATGTGAAGAG AGGAGATGAAGAGGATCAGATAGGTATCTTGCGAATCAAGGAAGAGATGGAAGCGAAGAGAATGTTGGAGAACAAGTTAA CTTCGATGACAACTATGTTTTCCTCGGAAAACGGAGACTCTGGTTCGAAATCAAGAAGCGTTATGATCGAACCAGGACGA AGATCGGTGTCTGACATCACTGCGGTTCCTAGACTCAGTATATCGATTCATGGAGATTGCAGCGGTTCAGCCGCAGAGAC TGCGTCTGCGTTACAAAACGGTGGGAATGAGACGGAGGAGAGAAGACGGCGTTTGTGGCTGCCGATCGCTAGAAAAACTG CTCAATGGTTTGCTAACAGAGAAAAAAGAAGTCAAATTAACACAACACACCAACACTTTGATGTTTAGTTTCTTTATTTT TCTTAAAAATTTGCAATTCTTTTTCAGATTCAATTTGAGACTATTTTCCCATAATTAGTAGATACAAGTTTTGATTTTAA GTTATGT ......N.....................................................................N... ................................................................................ .................................................i.............................. ............N................................................................... ...........................N.................................................... ................................................................................ ................................................................................ ................................................................................ ...............N............................................................i... ................................................................................ ................................................................................ .....................................................N....................N..... ........N.................................N..................................... .........i.................i..........................................N......... .....N........................................i..............i.................. .....N........N...............................................i................. .................................................i.............................. ..........................N..................................................... ....N.......................................................N................... ................................................................................ ...N... Pos Score Pred ------------------------ 7 0.443 - 77 0.207 - 210 0.626 Yes 253 0.441 - 348 0.332 - 656 0.160 - 717 0.717 Yes 934 0.162 - 955 0.207 - 969 0.324 - 1003 0.098 - 1050 0.545 Yes 1068 0.632 Yes 1111 0.141 - 1126 0.325 - 1167 0.525 Yes 1182 0.701 Yes 1206 0.179 - 1215 0.363 - 1263 0.734 Yes 1330 0.519 Yes 1387 0.231 - 1445 0.176 - 1501 0.111 - 1604 0.039 -

Explain the output -- Processing locus 488: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTCTTCGAATTGTCCAAGCTCCTATCGCAATTTCTCGATATGTTCCTAGAAGATTGATGCTTTAGAGTTTTCTCATAT AAATTTTGCAACCACTGATAAGCTTTCACTATTTTAATACAAAAATATATTAAAACCAGTTTAATCATTAATTTCATGTT TATATATAAGGACAAAAAAATCATAAAGAAGTTAGCTCTCAAACAAAGATATTGCTACTGCCTCACCAAATTTTCCTCCG GAAAGAGAGTGAGAAAATTTCCAAGAGAAAGAGGAGAGAGAATGGCTGGAGAAGAAATAGAGAGGGAGAAGAAATCTGCA GCATCTGCAAGAACTCACACCAGAAACAACACTCAACAAAGTTCTTCTTCTGGTTATCTGAAAACGCTTCTCCTGGTAAC GTTCGTCGGAGTTTTAGCATGGGTTTATCAAACAATCCAACCACCACCCGCCAAAATCGTCGGCTCTCCCGGTGGACCCA CCGTGACATCACCGAGGATCAAACTGAGAGACGGAAGACATCTGGCTTACACAGAATTCGGAATCCCTAGAGACGAAGCC AAGTTCAAGATCATAAACATCCACGGCTTCGATTCTTGTATGCGAGACTCGCATTTCGCCAATTTCTTATCGCCGGCTCT TGTGGAGGAATTGAGGATATACATTGTGTCTTTTGATCGTCCTGGTTATGGAGAGAGTGATCCTAACCTGAATGGGTCAC CAAGAAGCATAGCATTGGATATAGAAGAGCTTGCTGATGGGTTAGGACTAGGACCTCAGTTCTATCTCTTTGGTTACTCC ATGGGTGGTGAAATTACATGGGCATGCCTTAACTACATTCCTCACAGGTTAGCAGGAGCTGCCCTTGTAGCTCCAGCGAT TAACTATTGGTGGAGAAACTTACCGGGAGATTTAACAAGAGAAGCTTTCTCTCTTATGCATCCTGCAGATCAATGGTCAC TTCGAGTAGCTCATTATGCTCCTTGGCTTACATATTGGTGGAACACTCAGAAATGGTTCCCAATCTCCAATGTGATTGCC GGTAATCCCATTATTTTCTCACGTCAGGACATGGAGATCTTGTCGAAGCTCGGATTCGTCAATCCAAATCGGGCATACAT AAGACAACAAGGTGAATATGTAAGCTTACACCGAGATTTGAATGTCGCATTTTCAAGCTGGGAGTTTGATCCGTTAGACC TTCAAGATCCGTTCCCGAACAACAATGGCTCAGTTCACGTATGGAATGGCGATGAGGATAAGTTTGTGCCAGTAAAGCTT CAACGGTATGTCGCGTCAAAGCTGCCATGGATTCGTTACCATGAAATATCTGGATCAGGACATTTTGTACCATTTGTGGA AGGTATGACTGATAAGATCATCAAGTCACTTTTGGTTGGGGAAGAAGATGTAAGTGAGAGTAGAGAAGCCTCTGTTTAAG TCAATGTGGTTTTTAAGAATAATTAATTGCTTGCAATGCTAAACTAGTTATGATCAACAATCAAATCAAAGCAACTTTAG TCGTTAATACAAGTGTAC .........................................N................N..................... ...........................................................................N.... ................................................................................ .........................................i...................................... ................................................................................ ..................i............................................................. ................................................................................ .......................................N........................................ ...............................................N.......................N........ ....................................i........................................... i................N.....N........................................................ .......................................................N................i....... ...............N....................................i................N.......... ..............................N................................................. .................N.......................N...................................... ........................i...............i....N.....N............................ .......N..................i.............N....................................... ....i..........................................N................................ ...N...............................N.............N.............................. .................. Pos Score Pred ------------------------ 42 0.124 - 59 0.149 - 156 0.128 - 282 0.719 Yes 419 0.796 Yes 600 0.195 - 688 0.130 - 712 0.398 - 757 0.515 Yes 801 0.507 Yes 818 0.403 - 824 0.327 - 936 0.232 - 953 0.540 Yes 976 0.308 - 1013 0.638 Yes 1030 0.351 - 1071 0.241 - 1138 0.247 - 1162 0.427 - 1225 0.559 Yes 1241 0.647 Yes 1246 0.359 - 1252 0.199 - 1288 0.103 - 1307 0.603 Yes 1321 0.317 - 1365 0.617 Yes 1408 0.196 - 1444 0.067 - 1476 0.041 - 1490 0.081 -

Explain the output -- Processing locus 489: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAACAAAACAAGATTTAGCATAAACGTTCAAAATTGAAACTTTGATTCCTAACTTCTTGGAATGATTCAGATTGAGCTA GATTTAGATCTGTATGATTGATATTATGGGTTTGGTTTGTTTATGAGTAGAGAGAAAATGGGTGATGAGGCAGAGATCAA GGAACATTTAAAGCCACAAGCTTCATCTGAAACAATGGACAAGAAACATAATGTGAAAGGGAAGAGGTTATGGCAGAAAG TCAAGTATCAATTGGTGGAGTTTCATTCATTGCCTGCTTATTTAAGAGACAATGAGTACATCATTGGTCATTACCGATCC GAATGGCCGATCAAACAGATTCTTCTCAGCATCTTTACCATTCATAATGAGACTTTGAATGTTTGGACGCACTTGATTGG GTTTTTCCTGTTTTTGGCGCTCACTATATACACTGCAACGAAAGTACCGAGTGTCGTGGATCTTCATTCGCTTCAACACC GTTTACCCGATTTGTTGAGGAAAACAGATCTCCACAAACTTCATTCTGAGCTCATGGCTCGCCTTCCTTCTAGTCCATCT AGTTGGCATGTGATGGACCTTCTTTATAACTGTTTGCCTGAAAGATTTTCTCATGGCAACTACACTGACATGTGTGTTCT GCATTCTGTGAGGGAAGATCTTGCAAACTTGATAGCTCCTTTGATCTTCAGGCCAATTACTCGATGGCCGTTTTATGCAT TTCTAGGTGGTGCTATGTTCTGTCTATTAGCAAGCAGCACGTGCCACCTCCTCTCATGTCACTCCGAGCGAGTCTCCTAC ATAATGCTTAGGCTTGATTACGCCGGCATCGCAGCTCTAATAGCGACTTCCTTCTACCCTCCGGTTTATTACTCCTTCAT GTGTGATCCTTTCTTCTGCAACCTCTACTTAGGATTCATAACCATCTTAGGAATCGCCACTGTGCTTGTTTCTCTCCTCC CGGTTTTCCAAAGCCCGGAGTTTCGGGTGGTGAGGGCGTCTCTGTTCTTTGGAATGGGATTCTCTGGCTTAGCTCCGATT CTTCACAAGCTGATAATCTTTTGGGACCAACCTGAAGCCCTTCACACGACAGGTTATGAGATTTTGATGGGTTTGCTTTA TGGGTTAGGAGCTCTGGTTTATGCAACTAGGATCCCAGAGAGATGGATGCCGGGTAAATTCGATATAGCAGGACATAGCC ATCAGTTGTTTCATGTTCTGGTTGTTGCTGGTGCGTTCACGCACTATAGAGCTGGGCTAGTGTATCTTAAGTGGAGAGAT ATTGAAGGATGTTGAAGATGAAGATTGAAGATTAGATGGAATCTTTGAATCTTGTTTGTAGTAGCTTCATATAAAAGTTG GATTATGTAAAGTCTTATATGTAATCAAACGTTT ..............................................................i................. .............N...........N................i..............i......N............... ..................................i...............N..................N.......... ...................................................N............................ ..i...........................................N...........N..................... ................................................................................ .....................................................i.......................... .......N....N.......................................N................N.......... ...............................................................N..........i..... ..............N........................................N........................ ...N..........................................................................N. ................................................................................ .....................................................N.......................... .......................................................N..........N............N ....................N.....................N...i................................. ............N................................................................... ........N........N.................N............................................ ....N.............N............... Pos Score Pred ------------------------ 63 0.716 Yes 94 0.277 - 106 0.270 - 123 0.586 Yes 138 0.848 Yes 145 0.149 - 195 0.630 Yes 211 0.178 - 230 0.311 - 292 0.341 - 323 0.864 Yes 367 0.223 - 379 0.287 - 534 0.721 Yes 568 0.378 - 573 0.440 - 613 0.257 - 630 0.422 - 704 0.485 - 715 0.685 Yes 735 0.458 - 776 0.227 - 804 0.492 - 879 0.383 - 1014 0.485 - 1096 0.160 - 1107 0.236 - 1120 0.428 - 1141 0.106 - 1163 0.376 - 1167 0.571 Yes 1213 0.196 - 1289 0.333 - 1298 0.379 - 1316 0.080 - 1365 0.043 - 1379 0.056 -

Explain the output -- Processing locus 490: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTTATTTATATATTTCTACGTAAAGTTTTTGTCTTTTTTGTCTTGTTTGTTTTCACATTAGCTACTAGTGTTTTTTTTA TAAGTGGTGTGTTTGTTGTAAAGCTGTTTCACATGTGTAGCTTTTCGTTGTTCGAGTCCAGCTGCAGAATTAGTGTTTTT AACCAAAGCAATGTCTTCTGCTTCTTCATCTTCTGCTGCTTCTTTGTTGCTTGGAAGGGAGGTGGATGTCTTCCTGAGTT TCTGCTGCCAGACTAGCCATGGATATTTTCAAAATATTTTGATTCGAAATGGCATTAGAACTTTTTTATCCTACAGATGC TTGGAGAAGAGATTCGGACCAATTGGTCAACGGACACTCAAGGCTTTGGAAGAGTCGAGAGTCGCGGTTGTTATGACCTC TACGACGAAACCTTGCTCCGTTGGCTTCCTAGAAGAGCTCTTAGTGATACTCGAGTTTCAGGAAAAAGGTTCACTCATGG TCATACCCATTTTTCTTACAGATTTATCTTTTAATGTGGAAGAAATATGCAGACAACATCCGGAGAAGGCGCCAAGTTGG AGAACTGCACTTACCAAATTGACCAACCTTGCCGCTGAGTATCCATTATCTCAAAATCTTGCAGGAATGGATCAGTCAGA TCTACTCAACCAAATTGCTCGTGACATATCTCTTGTCGTGTTCTACTCCGGATCAAACGATTCGAATGCCCTTGTTGCAA TGGATCGTCACATGAAAGTGGTTTATGACTTGTTGGCTTTAGAAGTTAACAAAGAGGTTCGTACAATTGGCATTTGGGGT AGTGCTGGTGTTGGGAAGACAACACTTGCAAGGTACATTTATGCAGAGATCTTTGTAAACTTTCAGACACATGTTTTCCT AGATAATGTTGAAAACATGAAAGACAAGCTTCTAAAGTTTGAAGGAGAAGAAGATCCAACGGTAATAATAAGCTCATATC ACGATGGACATGAAATTACCGAAGCAAGGCGTAAACACCGGAAAATTCTCCTTATCGCTGATGATGTGAACAACATGGAA CAAGGGAAGTGGATCATTGAATATGCTAACTGGTTTGCTCCAGGAAGCAGAGTCATTCTTATTAGCCAGAACAAGAATTT GCTTGTTGACGCCGGAGTGATGGATGTGTACGAAGTCAGATCTTTAAGATATGATGAAGCTCTTCAAGTCTTCTCTCATT TTGCTTTCAAGCAGCCTTATCCACCTTCTGATTTCGAAGAGCTCGCAGTTCGTGCTGTCCATCTCGCAGGCTTTCTTCCT TTGGGCCTTAGACTGTTAGGGTCGTTTTTAGCTGGTAAAGGTCGAGAGGAGTGGGTAGCTGCACTGCTCAAACTCAAGGC AAAGCAAGGTGGACATATAATGGAAGTGTGGAAACTTATGGAAGCAACAGACGATAAAGGCCTAGAGGAGTGGGAAACTG CAGCCGATATAGTGGAAAGGAAGGAATCATCACAAGATAAAAGTCAACAAGAGAGTGAAGTTGCTGCAGACATTTTGATA GGAAAGGAATCATCACAAGATAAACAATAATAGTAGTGTGAAGAGTCATTTGTATCTCGTCTTTGTTTTATTGACGATAA TACGAGTGTGAAGCTTCCTCACTAAGCTTTAAGTTAGGACTCTGTTCTT ................................................................................ ................................N............................................... ..........i......................................................i.............. ..................N.............................i...........................i... ........................................................................N....... ............................................................................i... .................................N............i................................. ..................................................................i............. .................................................................N.............i ...........i............N....................................................... ........................................N.............................N......... .....N..........i............................................................... ...N.....N..................................................N..N..........i..... ......................N......................................................... ...................i...N..........................N..N.......................... ................................................................................ ................................................................................ ...................i.................N.......................................... ................................................................................ ................................................................................ ................................................. Pos Score Pred ------------------------ 113 0.196 - 171 0.668 Yes 226 0.585 Yes 259 0.446 - 289 0.675 Yes 317 0.536 Yes 393 0.100 - 477 0.514 Yes 514 0.209 - 527 0.676 Yes 627 0.725 Yes 706 0.335 - 720 0.852 Yes 732 0.647 Yes 745 0.359 - 841 0.146 - 871 0.288 - 886 0.339 - 897 0.564 Yes 964 0.302 - 970 0.101 - 1021 0.160 - 1024 0.379 - 1035 0.568 Yes 1063 0.064 - 1140 0.614 Yes 1144 0.404 - 1171 0.318 - 1174 0.241 - 1380 0.715 Yes 1398 0.368 -

Explain the output -- Processing locus 491: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTATGTTAGTCATTTACATAACACAAAAACTGCCGAGATCAGAGGACCGTTGGGACTTGACATTTAAATTTCAAAACGAT AAAAATACAAAATGAAATTAGAGATTGATGAAATCTAGGGCCAACGAAAATCAGATGATAAACAGAGAAACCATGAAAGG AAAGATTTTAGTTTCACCGTCGGAGAAATCAATGACATCGAGCACAACAACAACAACGACGACTACTACAACTGTATCTA CGGAAGATTGGGAGAGAAGAGACAGTAATTGTTACTTCCCAGACTGTCGTAAAGATGCGAATTGTAGCTGCGAGATTTGT CTTGATAGTCTCAACGCTACGCTTGATCTCATGCCTCTCAGTGTTCAAAAAAGCTCTCTCACTAAGCTTTCTTCTGCATC CAATTTCAAATCCACCGTTGAATCTACTCCGACTTCGTTTGATCCAACGGTGGTCACCACCCCTGCTTCTGTTTCTCGCC CAATCTTGAAGTTGATGATCTCTTCCCCGAAGAAGAAACTGACCAAGAAATCAAAAGTTTTCGAGAATGAAGAACAGAGG AAAACGACGAAGAAAGAGAGAAGACTACTGATTGTTGTTTTTCTGAAAGTGATTTTGGTGATTGGGTTGGTTCTGGGTTT GGAATTAGGGTTTAGTTGGGTGCTTGAAGAGGTTTTTAAACCTGAATTTACAGAGGAGATTGTGAGGAACGCTTATGAGA GAACTCAGGCTGATCTTGATTTGGGGGTGAAGCTGAGACTCTTGGAAGATGAATTTAAGGGTTTTGTTACGAGTAGAAAG TTTTCGAGATGCACAGGTTCTGATTCTAAATGGAAAATCAATCAGGATGGACCAATGTTGAACTCAAAGTGTGTTCTGTA CAAATCTGCCATTGAAGAGGTGAGTATATGGGGATGGCCTTTGCAGACATCTGGATTGTTCCATACCGGCTTCTCCTCGA GTTCGATCACCGTGTTATCAGGCCGAGTAACTGAGTGGACAGAGGGGAAATTCGGTTACTCAATACACGAGACCAACACT TCCTGGGGGAAAACCAAATGGAGCACATCGGTTCTGCAGCTAGATCCAAACACATGGGTTCTTGAGTACAGCCAAAGCTC GGTGATGGATGATAGTTCAAGTTTGTTGTCACTAACAATCGATATCATGGAACACTTGGTGTTCCGAGTAGCAAAGAATG TGAATCGGGAGCGTTATTGGATGTTTTCATCCTCACGACGATTGTATAGAGAAGCAGAGTCAAAGGCTAGCGCAATGACA CCAACTTAGAAACTTATGAGAAAAAAAAAATATTCCCCTATGTGGTTCCTTCGTATTAAGAGCATTGACATCTTGTCTTG TTTTAGGTATAGTTATGAACAAGTACTT ..N............................................................................. ...........i...............N..........................N.................N....... ...............................N................................................ ......................................................N......................... ..............................i................................................. ................................................................................ ..............N...................................................N............. ................................................................................ ..........................................................................N..... ................................................N............................... ........N....................N................N.......i......................... ...........................i.....N.............................................. ................................................................................ .................i...................................i.......................... ....N...N.....................................N..............................N.. ....................N.....................................................N..... ...............N.......................N........................................ ..............N............. Pos Score Pred ------------------------ 3 0.301 - 92 0.538 Yes 108 0.270 - 135 0.370 - 153 0.466 - 192 0.330 - 295 0.150 - 351 0.576 Yes 495 0.240 - 547 0.199 - 715 0.247 - 769 0.133 - 809 0.323 - 830 0.367 - 847 0.394 - 855 0.636 Yes 908 0.799 Yes 914 0.410 - 1058 0.672 Yes 1094 0.588 Yes 1125 0.498 - 1129 0.183 - 1167 0.433 - 1198 0.396 - 1221 0.301 - 1275 0.161 - 1296 0.103 - 1320 0.099 - 1375 0.041 -

Explain the output -- Processing locus 492: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTGACCATACGTAACTGTACCATACTTTTATGTTTCCACCATCAGGGCAACACAATAAAATTGAATGCCATCTAAAACA AAGCAAAAAGATTTACTATAATTATTTTCTTACTCATGCATGTACTCCCAATCTCCCATAGATATTTTCGAAGGTATTTT TGTGGTAACGTACAAGTCTTGGCTTCGTAATCTAAATACCGAAAAAGAAAATGCCTCTAACTTCCCTTCCTCTCTCTATA TATTTCAACCACTCTCTGTCTCTCTTCCTCTTTGTAGTTTCTCAAATATTTTCTTCAGAGTTGGCACGACCTCATTAATA ATAATCTTCTCTACACCTATCAATAAGAGATATATTTTAGAGTTTCACTTTGGTGCACAAACAAAGCTCGTACAACATTC TTCGTATATGGCTCTTCGTCGCTGCTTACCTCAATATTCAACAACTTCATCTTATCTCTCCAAGATCTGGGGATTTCGTA TGCATGGGACCAAGGCAGCAGCTTCTGTTGTAGAAGAACATGTCTCGGGGGCAGAACGTGAGGATGAAGAATATGCTGAT GTAGATTGGGACAACCTTGGATTCAGTCTTGTACGGACAGATTTCATGTTCGCCACCAAAAGTTGCAGAGACGGAAACTT CGAACAGGGTTACCTTAGCCGTTACGGCAACATCGAGCTCAACCCTGCTGCTGGAATTCTCAACTATGGCCAGGGACTAA TAGAGGGGATGAAAGCGTACAGAGGAGAAGACGGTAGGGTTCTTCTCTTCCGTCCAGAGCTAAACGCGATGCGTATGAAG ATAGGAGCTGAGAGAATGTGTATGCATTCTCCTTCTGTTCATCAGTTTATTGAAGGTGTTAAGCAGACCGTTCTTGCAAA CAGGCGTTGGGTTCCTCCTCCGGGCAAAGGCTCGTTGTATCTCAGACCGTTGTTGTTCGGAAGTGGAGCAAGCTTGGGTG TGGCTGCAGCATCAGAGTACACGTTTCTTGTGTTTGGCTCTCCTGTTCAAAACTACTTCAAGGAAGGCACAGCGGCGTTG AACCTGTATGTGGAGGAGGTGATTCCACGCGCTTATCTTGGAGGAACTGGTGGTGTAAAGGCAATATCCAATTACGGTCC AGTGCTTGAAGTGATGAGAAGAGCAAAATCAAGAGGGTTTTCGGATGTTTTGTATCTTGATGCAGATACTGGGAAGAACA TAGAAGAAGTCTCTGCTGCTAATATATTCCTTGTGAAGGGCAATACAATAGTGACACCAGCTACGAGCGGAACGATTCTC GGAGGGATCACACGTAAGAGCATAATCGAAATCGCCCTTGATCTTGGTTACAAAGTGGAAGAACGAAGTGTTCCGGTAGA AGAACTGAAGGAAGCTGAAGAAGTTTTCTGCACTGGAACTGCCGCTGGAGTTGCTTCTGTTGGAAGCATCACTTTTAAGA ACACAAGGACTGAGTACAAAGTTGGAGATGGGATTGTTACACAGCAACTTCGATCTATTCTTGTCGGAATACAAACTGGT TCTATCCAAGACACTAAAGATTGGGTTTTGCAGATTGCCTAAGTTACTTTCATATCCTATAAATAATGTTGCACTCTCTT GATCACCCATCAAAAGCTTTTGAAATAGTTTTTGGTAAATTTGTGACATAAATTTGTGATA ..............................N..................................N.............. ...................................N...N........................................ ..................................................i............................. ................................................................................ ................................................................................ .......i.......................................................................i ...N...................................i.......................N........N.....N. .............................................N.................................. .................................................................N.............. ........i...........................................................i.....N..... ...............i.....N.......................................................... ................................................................................ ................................................................................ .......N........................................................................ .............N..............................N..............N.................... ................................................................................ ................................................................................ ................................................................................ ...........................N.................................................... .................................................................N.............. ............................................................. Pos Score Pred ------------------------ 31 0.239 - 66 0.279 - 116 0.244 - 120 0.393 - 211 0.548 Yes 408 0.593 Yes 480 0.904 Yes 484 0.400 - 520 0.530 Yes 544 0.403 - 553 0.434 - 559 0.428 - 606 0.310 - 706 0.448 - 729 0.743 Yes 789 0.505 Yes 795 0.492 - 816 0.511 Yes 822 0.447 - 1048 0.143 - 1134 0.437 - 1165 0.080 - 1180 0.123 - 1468 0.336 - 1586 0.165 -

Explain the output -- Processing locus 493: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATATAAGGTCCAAAGAGCTATTATTCCATCTTACTTAAAAAAACACCGTCGTTTCGATAAGCCTCACATCTTAAATCCC ACCGGTAGACTGTAGTGATGAAACTCCGATGAAACAACAATGGCAGCGTTAAGCCACCGCTTACGTAGTTTCACACGCCG CTTCTCCTCCACCCGGACCACTCAAAGAAGCGGCGGAGGAGCCAACACCAAGGTACCTATTCTCTACACAAGCCCAGACA TCGACTCCGATCCCGTCACTCTCCAGCTCTTCTCGTGGGGACGTGGCGCGTCAGGTCAGCTCGGCGGCGGAATCGAGGAG ATTCGAATGTATCCGTCTCCAGTCGCTAATCTTCTCTTTCGCTCTGATCAATCCTTCTCTCTCGCTCAAACTCCTGGAAG AATCGACGCAGATTCTTCCAGTTTTCGAATTGGAATCTCTTGCGGCTTGTTCCATTCTGGTTTAACTATAGACGGAGATC TATGGATTTGGGGGAAAGGTGACGGAGGAAGGTTAGGGTTTGGCCAAGAGAATTCGGTTTTCGTCCCCAATTTAAATCCG CTTTTTGAGGAACATTCGATTCGATGTATAGCTCTTGGTGGTTTGCATTCAGTAGCTTTAACACATCAAGGAGATGTATT CACTTGGGGTTATGGTGGTTTTGGAGCGCTTGGTCATAAGGTTTACACTAGAGAACTCGTTCCTCGACGTGTTGACGACT CTTGGGATTGCAAAATCTCTGCCATTGCTACTAGTGGAACTCATACCGCTGCTATTACTGAATCAGGGGAGCTTTATATG TGGGGAAGAGAAGAAGGAGATGGTAGATTAGGACTCGGGCCAGGCCGAGGACCAAACGAAGGTGGTGGGCTTAGTGTTCC TTCAAAGGTCAAAGCTTTAACAGTTCCTGTAGCCTCTGTCTCATGTGGTGGTTTTTTCACAATGGCTCTTACAAAAGAGG GACAACTCTGGAACTGGGGAGCAAATTCCAATTATGAACTCGGACGCGGTGATAACTTGGGAGGTTGGGAACCAATGCCG GTTCCCAGTTTAGAAGGGGTTCGTATTACCCAGATTGCGTGCGGTGGATATCATTCTCTTGCCTTAACCGAGGAAGGTAA AGTACTCTCATGGGGCCACGGTGGCCACGGCCAGTTGGGTAGTTCCTCGCTGCGAAACCAGAAAGTTCCTACTGAAATCG AAGCATTAGCTGACAAAAAGATAGTCTTCATCGCTAGTGGAGGTTCCTCTTCTGCAGCGATAACAGGATGGGATTGGTTT TTGACAGATGGGGGTGAACTCTGGATGTGGGGAAACGCTAAAGATTTCCAGCTAGGAGTTCCAGGTCTCCCTGAGATCCA AACTACTCCGGTCGAAGTCAATTTCTTAACCGAAGAAGATGAGTGTCAACCACACAAGGTTATCTCAATTTCCATTGGTG CTTCTCATGCTCTGTGTTTAGTCTCAAGATCACCTTAAGTAACACTTGGATAGGCGGTACCGTTCCTTAGAAACGCAGAA CATTATGGTTCAGCTCAAAGGTTGTTTGGGATATACATTTGATATGGAAACGCAGTG ................................................................................ .................i..........i..........i........................................ ................................................................................ ................................................................................ ......i......................................................................... ................................................................................ .......................N.................................................i...... ...........N.................................................................... .............................................................................i.. ...................N............................................................ ..........................................N..................i.................. .................................i........................................i..... ................................................................................ .........N...................................................................... ...................................................................i............ .......N................i....................................................... ......................................i......................................... ......N......................................................................... ....N......................................N............. Pos Score Pred ------------------------ 98 0.680 Yes 109 0.517 Yes 120 0.818 Yes 327 0.647 Yes 482 0.455 - 584 0.235 - 634 0.509 Yes 652 0.321 - 798 0.701 Yes 820 0.253 - 923 0.330 - 942 0.659 Yes 994 0.584 Yes 1035 0.714 Yes 1130 0.405 - 1268 0.517 Yes 1288 0.384 - 1305 0.524 Yes 1399 0.684 Yes 1447 0.173 - 1525 0.121 - 1564 0.090 -

Explain the output -- Processing locus 494: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATACAACCGCAAAACAATTACAATTTTGGCTTGTAGTTAGGCTAAATTTTTGGCTTATATATAAAATTAAAAAATAAAA TAAAATATATGACACAATTAGTTCCACATGAGAAAAAAAATCATGAACTGCATGCCGGTTTCTTATAAACAAAACGGAAG ACAGTGCAAATCTCAAATCCCGAAACTTGACGCGCGCCTCTCTGTCTCTCTCAATCAAATTCAAATCAAAATTTCAAAAA AGCGGAATCGGAAAAAAAATCTCAAAATGGAGGAAAGTTCAGGACTATCATCGATGAAGTTCTTCTCCGATCAACACCTC TCTTACGCTGACATTCTTCTTCCTCACGAAGCTCGTGCTAGAATCGAAGTTTCTGTTCTCAATCTCCTCCGAATTTTGAA CTCCCCAGATCCAGCTATCTCCGATCTCTCTCTGATCAATAGGAAGAGAAGCAATAGTTGTATAAACAAAGGGATACTCA CAGATGTTTCATATATATTCCTCTCTACTTCGTTTACTAAGAGTTCATTGACGAATGCTAAAACAGCTAAAGCTTTTGTT CGTGTGTGGAAAGTTATGGAAATATGCTTTCAGATTCTGCTTCAAGAGAAACGAGTCACACAAAGAGAGCTCTTCTATAA GTTGCTTTGTGATTCACCTGATTACTTTTCATCTCAGATTGAAGTTAACAGAAGTGTCCAAGATGTGGTAGCACTTCTAC GTTGTAGTAGATACAGTCTTGGTATTATGGCTTCAAGCAGAGGCCTTGTTGCTGGAAGGTTATTTCTACAAGAACCAGGT AAGGAAGCTGTAGATTGTTCGGCCTGTGGTTCTTCAGGTTTTGCTATAACTGGAGACTTGAATTTGCTAGACAATACCAT CATGAGAACTGATGCTCGTTATATCATTATTGTGGAAAAGCATGCGATCTTTCATCGGCTCGTGGAAGATCGTGTGTTCA ATCACATTCCTTGCGTGTTCATCACAGCGAAAGGGTATCCGGATATTGCCACAAGGTTTTTCCTCCACCGGATGAGCACA ACTTTTCCTGATCTGCCAATTCTTGTTCTAGTTGATTGGAATCCAGCTGGGTTAGCTATACTATGCACCTTCAAGTTTGG AAGCATAGGGATGGGACTTGAAGCATACAGATACGCTTGCAATGTGAAGTGGATTGGTCTCCGAGGAGATGATCTGAATC TGATACCAGAAGAGTCTTTGGTTCCCTTAAAGCCAAAAGATTCACAGATTGCTAAGAGCTTATTGTCCTCCAAAATATTG CAGGAAAACTACATAGAGGAGTTGTCACTGATGGTTCAAACTGGTAAAAGAGCGGAAATTGAAGCTCTCTATTGTCATGG TTATAATTATCTCGGTAAATATATAGCTACCAAGATCGTGCAAGGCAAATACATATAACACTTAAAACACCAACATGTAA GGTCTTCCACCAATAAAGATGCGTTTGCTTTATATTGGTTCCAAATATAAGAGAGTAACTTATTAGGGTGAAGTTTT ................................................................................ ........N..................N..............i........N............................ ................................................................................ ..........................i..........................N.......................... ................................................................................ ................................................................................ ...N..................................................N......................... ...............N.......N........................................................ ..............................................................N................. ..........................i..................................................... ................................................................................ .......................................................................N........ ..............................................................N................. ..........i..............................N..........................N........... ................................................................................ ..............................i.............................................N... ..........................................................................N..... ..................N.......................................................... Pos Score Pred ------------------------ 89 0.315 - 108 0.356 - 123 0.527 Yes 132 0.339 - 267 0.881 Yes 294 0.494 - 484 0.175 - 535 0.248 - 576 0.285 - 584 0.380 - 703 0.465 - 747 0.719 Yes 882 0.629 Yes 892 0.279 - 922 0.219 - 1032 0.406 - 1103 0.457 - 1131 0.672 Yes 1162 0.296 - 1189 0.221 - 1311 0.773 Yes 1357 0.320 - 1435 0.170 - 1459 0.095 -

Explain the output -- Processing locus 495: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGTAATCACAGACAGAGGAAAAATAAGAATAAGGTGCAGTTTTTTTGTTCTTTGTTGTTATTTAAGAAGATTAACGTAA ATAGTTTCCGACATTAACTAGTTTGCGTTACATTGGTCCTGGTTGATAATGAAGAAGATGTACAATCTTCCAAGAGATTT GCCGGAGGAGGTGCTCTGTAGAATTCCGTTGACATCTCTGAGGCCTGTCCGATCTACTTGCAAAAAGTGGAGCACTTTAT CCAAATGTGGGAGCTTTGCAAAGAAGCACCTTGCTCAAGCAAAAGTACTAGCAGACGCAAAGGAGTTTATGGTGGTCTTG ATGATGAATTTTAGGGTTTATTTGATGAGAGTCAATCTTCAAAACAACGTTGTTGAATCATCATCATCATCATCATCATC ATCATCATCATGTATAAAACGTGAAGCTAAACTTATTAGCTTAGGCGATGAAGAAGCTGATATATCTCAAGTCTTTCACT GTGACGGTTTATTGTTATGCATCAGCATCACCGAATCCAAGACTAGGCTCGTTGTTTGGAACCCGTATTGGGGGCATACC CGGTTATTCGAGCCCACACATCAATTCAACAAATTCGATAGCTATTCGTATGCTCTCGGATACGACAAGAGCCGCAAATC CCACAAAATCTTGAGGGGTATTACTTGTTTGGATCCCTTCAAAATCTACGATTTTAACTCTGACTTATGGAGGGATCTTG ATGTAACTCCGGAGTGGCACTTATGGCAAATGCTCCATGGCGTGTCTCTCAAGGGAAATGCCTACTGGTTTGCTAGAGAG AATTATACCGAAACAATGGACACAGACCATTTTTTCTTACTCTGTTTTGATTTCACAAGCGAGACATTTGGGCCGCCTTT GCCTCTTCCGTTTGAGTTTGCTGTTTCTGAAGATACTATGAGTGTATCTAGTGTTAGAGAAGAGCAGCTTGTTGTATTAT ATCAGCCGTGGGATTATTTACAGTTGGACATCTGGGTGACGTCTAAGATTGAGCCCAACGCTGTGTCGTGGAACAGCAAG GTGTTCTTATCAGTGAGTTTAAAACAACTCGTCAGCCCTCAGTTTCAGTTAACTTTTGGGAGTTTCTTCATCGACGAGGA GAAGAAAGTCGCTGTGGTTTTTGATAAAGACTACGATAATAAGCGCAACATAGCTTACATCTTTGGAGTGGATGGATCCT TTAAAGCAGTTGATCTCGGAGATTCAGACCGAAAGTGTTTTCCACTTGTGTGCTCTTATGTTCCAAGTCTAGTGCAACTT AATTAGTCGACCATCTATTCCTTGCTTACTTTTTTTCTTCCTCTTTTGTCATCTTCTTTGAACAAGATCATTATTTGTTA GTCAATAACATATTAACTTGTTTCT ................................................................................ ................................................N........i...................... ................................................................................ ....N...............................................................N........... N..N....................N....................................................... .........N.....................................i................................ ................N............................................................... .................................................N.............................. ..................................................................N............. N.....................N......N......N....................N...................... ...............i................................................................ .....................................i.......................................... ................................................................................ ................................................................................ .......................................................................N........ .........................................................N...................... ................................................................................ ......................... Pos Score Pred ------------------------ 129 0.498 - 138 0.505 Yes 245 0.375 - 309 0.311 - 321 0.124 - 324 0.151 - 345 0.131 - 410 0.315 - 448 0.621 Yes 497 0.311 - 610 0.316 - 707 0.466 - 721 0.212 - 743 0.430 - 750 0.228 - 757 0.355 - 778 0.186 - 816 0.754 Yes 918 0.621 Yes 1192 0.171 - 1258 0.118 -

Explain the output -- Processing locus 496: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 497: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCAATTGTGATCAGCTAATTTAATTTTAGAATATTTTGTACTTCCATTCCATGGCATGTCATATGAAGATATGAAAATTA GTAAATTTATTGGGCTTATAAATGGGCCTTATATTATTAATGGGCCTTATATTTATTTTTCATATGATATTTCTGGAGAA AGCGACAGTATATTATCTTCAATTTTTCCATTTTGAAGAATTTTCTCAAGAAAAAAAAAAATCAATGTGGAGAAGATCAC CGTCGTTTATTCTCGACGAGCGACGAAGCTCGAATTCACCTCCAATGGCGGATACAACACGAGGTCCTTTTCAAAACCCT GATTCCATCTCCGCTTATTACCAAACTCGCGCGGCGCATCACGGTGTCATTACAAGCGACTGGCTAGCCCAAGCTCAAGC CGCCGTTGGCGGCGTCTCAGGCGATGCCCAGCATGACTTATCCGTAACTGATCTCGGGAACGAGAAGTCGTTTAATGTAA TTGAAGAGTTCAATAACTGGAGAAAACAACCGGATCTGGCTGAAGCCGTCGCGGCTATACGAGCTTTGGCTGCTGTTATT CGTGCGAGCGAAGCGAGCACCATGATGGAGCTTGAAATTGAGCTCAAAAAGGCTTCTGATACACTCAAGTCGTGGGACAA AACGTCGATCTCTTTGACAGCTGGATGCGATTTGTTTATCAGATACGTTACGAGAACGTCTGCTTTGGAATATGAAGATT TCAATTCAGCTAAGTCTCGTCTTCTTGAACGTGCTGAGAAATTTGGGGAAATATCTTGCAAGGCTCGTAGGATTATCGCA ATGCTTAGTCAAGATTTTATATTCGATGGTTGTACCATATTGGTTCATGGACTCTCTAGAGTTGTTCTGGAAATACTTAA GACTGCTGCACAAAACAACAAGCTCTTTCGAGTTCTCTGCACAGAGGGGAGGCCGGATGGAACAGGAGTTCTGTTATCGA GTGAGCTATCGAAGCTCGATATCCCAGTGAAACTACTGCTTGATTCAGCTGTGGCGTATAGTATGGACGAGGTAGACATG GTGTTTGTCGGGGCGGATGGAGTTGTAGAAAGTGGCGGTATTATCAACATGATGGGAACATACCAAATCGCACTTGTGGC ACACAGCATGAACAAACCGGTCTATGTTGCTGCTGAGAGTTACAAGTTCGCTAGGCTTTACCCATTAGACCAAAAGGACA TGGCACCGGCGTTGAGACCAATCGAATTTGGTGTAAAGATTCCGACAAAGGTGGAAGTAGAGAGGTCAGCTCGAGACTAT ACTCCGCCTCAGTACCTAACACTCCTCTTCACTGACCTTGGTGTTCTCTCTCCCTCTGTAGTTAGCGATGAGCTTATTCA ACTCTATCTCTAATTTCAACTCCTAATTAATAACTAACTATTTATACTACTTTTGCTTCAGAAGTGAAAACTTCCAATTT TGAGCCTTTTAAGATCTTCAAAGTTCATGTTT ..................................................N....i......N.......N......... .....................N.................N.......................N................ ................................................................i............... ............................................i................................... ................................................................................ .......................N........N.........................................N..... ................................................................................ .....................N..N....................................................... ........................N..............................................N........ ................................................................................ i........................N....................N................................. ........................................................N....................... ..............................................................i..............i.. ................N...............................i..i............................ .......N...............N.......................................................i ................................................................................ ...................................................................N............ ................................................................................ ..........................N..... Pos Score Pred ------------------------ 51 0.176 - 56 0.556 Yes 63 0.145 - 71 0.441 - 102 0.288 - 120 0.212 - 144 0.096 - 225 0.717 Yes 285 0.714 Yes 424 0.264 - 433 0.177 - 475 0.195 - 582 0.210 - 585 0.416 - 665 0.280 - 712 0.262 - 801 0.518 Yes 826 0.367 - 847 0.487 - 937 0.215 - 1023 0.744 Yes 1038 0.637 Yes 1057 0.167 - 1089 0.527 Yes 1092 0.544 Yes 1128 0.400 - 1144 0.145 - 1200 0.674 Yes 1348 0.346 - 1467 0.031 -

Explain the output -- Processing locus 498: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATAAAGGGATGAAACATGTCGCCTTATCATTGTTTTAGTGAGGATACAAAGACTCTAAATAAGTCGGAAGGTTCTAACT CTACCTGGTACATCTTCGTAGCTACAAAACAAAACTCGCCGAGACATTAACCAAATGACAAATACGAACACACTCTTCCT CAGACATGATGATGATTCAGAGATTTTGATTTTTCAAGAAAACTCTGTTTTTCCTCTTTTTGTTTTTGCCTCTTGGGGCA TTTGGAGAGCAATGGCGACTTTTGCTGAACTTGTTTTATCGACTTCTCGCTGTACATGCCCTTGCCGTTCATTCACTAGA AAACCCCTAATTCGTCCCCCTTTATCTGGTCTGCGTCTCCCCGGTGATACCAAACCATTGTTTCGTTCCGGACTTGGTCG GATTTCTGTTAGCCGGCGTTTCCTCACGGCCGTTGCTCGAGCTGAATCAGACCAGCTTGGTGATGATGACCACTCAAAGA ATGTGGAAGATAAGCAGAAGAAAGCAAGCCAGCTTAAGAAAAGAGTGATCTTTGGTATTGGCATTGGTTTACCTGTTGGA TGTGTTGTGTTAGCTGGAGGATGGGTTTTCACTGTAGCTTTAGCATCTTCTGTTTTTATCGGTTCCCGCGAATATTTCGA GCTTGTTAGAAGTAGAGGCATAGCTAAAGGAATGACTCCTCCTCCACGATATGTATCTCGAGTTTGCTCGGTTATATGTG CCCTTATGCCCATACTTACACTGTACTTTGGTAACATTGATATATTGGTGACATCTGCAGCATTTGTTGTTGCAATAGCA TTGTTAGTACAAAGAGGATCCCCACGTTTTGCTCAGCTGAGTAGTACAATGTTTGGTCTGTTTTACTGTGGTTATCTCCC TTCTTTCTGGGTTAAGCTTCGCTGTGGTTTAGCTGCTCCTGCGCTTAACACTGGTATCGGAAGGACATGGCCAATTCTTC TTGGTGGTCAAGCTCATTGGACAGTTGGACTTGTGGCAACATTGATTTCTTTCAGCGGTGTAATTGCGACAGACACATTT GCTTTTCTCGGTGGAAAGACTTTTGGTAGGACACCTCTTACTAGTATTAGTCCCAAGAAGACATGGGAAGGAACTATTGT AGGACTTGTTGGTTGTATAGCCATTACCATATTACTCTCTAAATATCTCAGTTGGCCACAATCTCTGTTCAGCTCAGTAG CTTTTGGGTTTCTTAACTTCTTTGGGTCAGTCTTTGGTGATCTTACTGAATCAATGATCAAGCGTGATGCTGGCGTCAAA GACTCTGGTTCACTTATCCCAGGACACGGTGGAATATTAGATAGAGTTGATAGTTACATTTTCACCGGCGCATTAGCTTA TTCATTCATCAAAACATCCCTAAAACTTTACGGAGTTTGATGATCTACCGGAAAGACCATATCTTTAGGAAACTTACAAG CATATCCATGGTAAGATCCGGCAATGTAGCTGTGAAACAAATGGTGCAGTGTTCTCTGT .........N......i............................................................... ......................................................N......................... .....N..N..N.................................................................... ...........i...........................................N........................ ................................................................................ ..............................................................N..N.............. N..............................................................................N ....................N........................................................... ...............................N..................N........................N.... .....N.......................................................................... ................................................i............................... ..................................................................i............. ................................................................................ ..............................................................N................. ................................................................................ .....................................................i............i............. ................................................................................ .......................................N........................................ .......N...............N................N.................. Pos Score Pred ------------------------ 10 0.315 - 17 0.825 Yes 135 0.400 - 166 0.252 - 169 0.298 - 172 0.275 - 252 0.517 Yes 296 0.316 - 463 0.117 - 466 0.088 - 481 0.335 - 560 0.170 - 581 0.313 - 672 0.445 - 691 0.120 - 716 0.336 - 726 0.403 - 849 0.659 Yes 947 0.571 Yes 1103 0.455 - 1254 0.547 Yes 1267 0.568 Yes 1400 0.112 - 1448 0.069 - 1464 0.047 - 1481 0.038 -

Explain the output -- Processing locus 499: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACATTAAACAAATACAAAACCATATCATAAATACGTTGCATCTTACTATAAATACAAAAGACCACGATCAATAGTTTT ACTCAAACAACTACAACTTAAAACATTATGTCGATGGAGGAGATTAGTAGCTGTGAGAGCTTTCTCACAAGCTCTAAACC CTACTTCGCCATGATATCTCTCCAGTTTGGCTACGCCGGCATGAATATAATTACCAAAATATCCCTCAACACCGGTATGA GCCACTATGTCCTTGTCGTCTACCGCCATGCAATTGCCACCGCCGTTATTGCACCCTTTGCTTTCTTCTTCGAAAGGAAG GCTCAGCCGAAAATAACGTTCTCCATTTTCATGCAACTATTCATTCTCGGCCTTCTTGGGCCAGTGATCGATCAAAACTT CTACTACATGGGGCTGAAATATACGTCCCCAACGTTCTCATGCGCCATGAGTAACATGCTTCCGGCCATGACCTTTATCT TGGCTGTACTATTCCGGATGGAGATGTTGGACCTGAAGAAACTGTGGTGTCAGGCGAAAATAGCCGGAACGGTAGTGACA GTGGCGGGAGCGATGCTAATGACAATTTACAAAGGACCCATCGTTGAGCTATTTTGGACCAAATATATGCATATTCAAGA CTCTTCACATGCTAATACGACGTCGTCTAAGAACTCATCAAGTGATAAGGAATTCCTCAAAGGCTCCATCCTCCTAATCT TTGCAACACTCGCTTGGGCCTCCCTCTTTGTTCTACAGGCAAAGATACTAAAGACGTACGCGAAGCATCAGCTTTCTCTA ACAACACTAATTTGCTTCATCGGAACACTACAAGCAGTTGCTGTGACGTTTGTGATGGAACACAACCCCTCTGCTTGGAG AATTGGCTGGGACATGAACCTCCTTGCCGCCGCTTACTCTGGGATTGTGGCATCAAGTATCTCATACTATGTGCAAGGGA TAGTGATGAAGAAAAGAGGACCTGTGTTTGCTACTGCCTTTAGTCCTTTGATGATGGTTATTGTTGCTGTCATGGGTTCT TTTGTCTTGGCAGAAAAAATCTTCCTTGGAGGGGTGATTGGTGCGGTACTGATTGTAATTGGACTGTATGCTGTCTTATG GGGAAAGCAAAAGGAGAATCAAGTGACTATATGTGAACTAGCCAAGATTGACTCAAATAGTAAAGTAACTGAAGATGTGG AAGCTAATGGATCTAAAATGAAAATCTCGGAAGGGGATAACTCTATGCTCTCTACGATTGTAATCAGCGTACCACTGTCG GAAACACATTTGAAAAAGACTATCCAAGAGCCATGAAGATTGTATGGCACTACATATGAAAAATAAAACTAAAGGCTGTT TTGGTCGAATCAGTATTTTTGTCTTATAATTTTGACCTCAGTAAGAGAAAGAGCT ................................................................................ ...........................N.....i.............................................. ..........N.............................i...................................i... ......N....................N.................................................... ..............................N................................................. .......i...............................i......N........i...........i............ .................N.....N........................................................ ............N.....N...............................................N............. ........N....................................................................... ................................................................................ ......................................................i......................... .............N......................................................N........... .....i............................................N..i.................N........ ...................................................................N.........N.. ..............................N...........................................N..... ......N..........N..........................N................................... ................................N..........N...........N........................ ....................................................... Pos Score Pred ------------------------ 108 0.460 - 114 0.538 Yes 171 0.324 - 201 0.725 Yes 237 0.578 Yes 247 0.487 - 268 0.138 - 351 0.123 - 408 0.527 Yes 440 0.669 Yes 447 0.488 - 456 0.505 Yes 468 0.577 Yes 498 0.373 - 504 0.248 - 573 0.487 - 579 0.167 - 627 0.416 - 649 0.202 - 855 0.590 Yes 894 0.421 - 949 0.087 - 966 0.618 Yes 1011 0.280 - 1014 0.572 Yes 1032 0.467 - 1108 0.163 - 1118 0.265 - 1151 0.254 - 1195 0.224 - 1207 0.115 - 1218 0.364 - 1245 0.371 - 1313 0.271 - 1324 0.171 - 1336 0.063 -

Explain the output -- Processing locus 500: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATCTTGAGTTTGTGTCTGGAGTTTTGTCTCAACAATACCAAAGACCATAAAGACTTTGAGGTGATTTGAGTTCAGATT CTTCTGAATCTCCAATGCTAAGTTTCTCTAGAGCTAGAAGTCAAGGAAGAAACACAAGACCTCTTGGAGGAGGTTAGTCT TGAATCACGATATTTATATATTTTAAGTATAATGAGTCTCTGCAACATTTTAGATGATCAATCATTTTCTTTTGATTTTG ATAATTCAGGGATGGAATACTTAGAACCTAAAAGGAAGAGCAATGTAATGGGCAAGATTATCTTGGTTGTTTCTCTTACT GCTTTATGCATTTTTATGCTCAAGCACGCACCGAGTTTCACATCACCAACCGCGTTCTCTCGTAGCGAGGAAGGTGTGAC TCATGTGTTAGTCACTGGTGGTGCTGGATATATCGGATCACATGCTGCGTTGAGGCTCTTAAAAGATTCATACCGTGTAA CCATTGTGGACAATCTTTCTCGGGGAAATCTTGGTGCCGTCAAGGTTTTACAAGGATTGTTCCCAGAACCCGGGAGGCTT CAATTTATTTATGCTGACTTAGGAGATGCCAAAGCTGTTGATAAAATCTTCTCAGAGAACGCGTTTGATGCTGTTATGCA TTTTGCTGCAGTAGCATATGTTGGAGAGAGCACTCTAGATCCTCTAAAGTATTACCACAACATTACATCAAACACATTAG TAGTTCTTGAAGCTGTGGCTAGACATAAAGTGAAGAAGTTGATATATTCAAGCACATGTGCCACTTATGGAGAACCTGAC AAGATGCCTATTGTTGAAGTTACTCCACAGGTCCCCATTAACCCTTATGGAAAAGCTAAGAAGATGGCTGAAGATATGAT CCTGGATTTCTCTAAAAACTCTGACATGGCAGTCATGATCCTAAGATACTTTAACGTGATAGGTTCAGATCCAGAAGGTA GACTAGGAGAAGCTCCAAAGCCCGAGCTACGTGAACACGGACGGATTTCGGGTGCCTGTTTCGATGCAGCTCGTGGCGTG ATTCCAGGCCTTCAGGTCAAAGGAACAGACTACAAAACAGGAGATGGAACTTGTGTTCGTGACTACATAGATGTTACTGA CCTTGTAGATGCTCACGTAAAAGCTCTCGAGAAAGCTAAGCCTAGAAATGTGGGAATATACAATGTAGGTACAGGAAAAG GAAGATCGGTGAAAGAGTTTGTGGAAGCATGTAAGAAAGCGACAGGAGTAGATATCAAAGTAGATTTTTTGCCTCGAAGA CCTGGAGATTACGCAGAGGTTTACAGTGATCCAGCAAAGATTCTGAGAGATCTTAATTGGTCTGCTCGTTACACTAATCT TCAAGAAAGTCTTGAGGTTGCTTGGAAGTGGCAAAAGACTCATCCCCATGGATATGCTTCTTCTTAAAGACGACCAAGAA AGAAAAAAAAGTTTTAATTTTAAACTTTTTACATAATTGTTTTTGAGAGATTAGCTTATAGAAAAAGAATTGATTTATTA CAGAAT ................................................................................ ..............N................................................................. ...............................N.....................N.......................... ...........i..............................N....i................................ .....N.........i................................................................ ..N......................................N...................................... ................................................................................ ..........N..............N.........................................N.......i.... .................N.............................................................. .......................................................N..........N............. ...i..........................................N................i...........i.... .........................i........i............................................. ...............................................................N................ ...........................................i..........................N......... ........N......................................N..............N................. ............................N................................................... ................................................................................ ...............................................N.....N.......................... ................................................................................ ...... Pos Score Pred ------------------------ 95 0.395 - 192 0.481 - 214 0.167 - 252 0.840 Yes 283 0.151 - 288 0.611 Yes 326 0.226 - 336 0.507 Yes 403 0.259 - 442 0.121 - 571 0.224 - 586 0.299 - 628 0.117 - 636 0.547 Yes 658 0.097 - 776 0.150 - 787 0.204 - 804 0.788 Yes 847 0.142 - 864 0.746 Yes 876 0.739 Yes 906 0.600 Yes 915 0.608 Yes 1024 0.239 - 1084 0.501 Yes 1111 0.378 - 1129 0.237 - 1168 0.267 - 1183 0.465 - 1229 0.184 - 1408 0.235 - 1414 0.118 -

Explain the output -- Processing locus 501: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTATGCCCAAAATTTATTTTAGTTGAAGAAAAAGGGTTTACGATGCTAACCTAGCTAGTTCGTTATTAAATGTTCTAGA CTTGACTATTGGCCATTTTGAATAAAAAGTCCTCATATGTTAAATTTTCACATGTCGATCCAAGCTCTACATATATATAT TTACTCATCATTCCAAAAGCTTCCAAAGAAACAAAGAGTAACAATGAAACGAAACAGTATAAATATTCATCACGTTACTT CTTTTTCTTCGAGTCCGTTTTGGTGTGTTTTCTTTCTCGTGTTGTTATGTAAGACGTCCACCAACGCTTTGGTGAAGCAG CCGCCAAACGAAACGACTCCGGCGATAATTGTGTTCGGAGATTCAATCGTTGATGCCGGAAATAATGACGACATTATGAC AACGTTGGCTAGATGCAATTATCCTCCTTACGGCATCGATTTTGACGGTGGAATTCCTACCGGAAGATTTTGCAACGGCA AAGTTGCGACTGATTTTATAGCGGGAAAATTCGGAATTAAACCAAGTATACCGGCATATCGAAATCCAAATTTGAAACCA GAAGATCTCTTAACCGGTGTAACATTTGCGTCTGGTGGTGCTGGTTACGTTCCCTTCACAACCCAATTATCGACGTACCT TTTTATATATAAACCATTGTTGTTCTTAAAGGGAGGAATAGCTTTATCGCAGCAACTGAAGTTATTCGAAGAGTATGTAG AGAAGATGAAGAAAATGGTTGGGGAAGAAAGGACAAAGCTCATAATTAAAAACAGTTTGTTCATGGTTATATGTGGCAGT AATGATATAACCAACACCTACTTTGGTCTTCCTTCTGTTCAACAACAATACGACGTCGCTTCCTTTACCACTCTTATGGC CGACAATGCTCGATCTTTTGCTCAAAAATTACATGAATATGGAGCGAGGAGAATACAAGTATTCGGTGCACCACCAGTAG GATGTGTCCCATCACAGAGAACCTTAGCAGGAGGGCCAACAAGAAATTGTGTTGTTAGGTTCAATGATGCAACAAAGCTC TACAATGTTAAGCTCGCTGCAAATTTGGGTTCTTTGTCAAGAACTTTAGGAGACAAGACAATAATCTACGTTGACATATA TGATTCTCTCCTTGATATCATTCTTGACCCTCGACAATATGGATTTAAGGTGGTTGATAAAGGATGTTGTGGAACTGGAC TAATTGAAGTTGCTCTTTTATGCAACAACTTTGCAGCTGATGTATGTCCAAATAGAGATGAATATGTGTTTTGGGATAGT TTCCATCCGACTGAGAAGACTTACAGAATTATGGCCACAAAATATTTTGAGAGATATGTATGATCTCGACAATCGTGTAA GTCATAGCTTGATTTATCGTGTCTTTTGATGTTTATTATCTTATTAGACGTGACCTCGAAATGTTTTTCCAAAAGAATAT GA ...N.......................................N..........................N......... ....................................N..............N............................ ...........................................N.................................... ..............................................N................................. ....................................................N...........N..........i.... ............N................................................................... ................................................................................ ................................................................................ ..........................................................................N..... .....N........N...............................................N.......N......... .....N..........................i.....N......................................... ....N..........................................................................N ......................................N........................N................ ...................N...................N...N.............N.....N................ ..............................N........................N...N.................... ............................N...............................N.................N. .. Pos Score Pred ------------------------ 4 0.372 - 44 0.241 - 71 0.253 - 117 0.141 - 132 0.427 - 204 0.458 - 287 0.493 - 373 0.129 - 385 0.303 - 396 0.639 Yes 413 0.157 - 715 0.280 - 726 0.261 - 735 0.437 - 783 0.406 - 791 0.154 - 802 0.254 - 876 0.684 Yes 886 0.260 - 913 0.510 Yes 919 0.462 - 962 0.370 - 1024 0.193 - 1027 0.325 - 1045 0.094 - 1120 0.232 - 1159 0.328 - 1184 0.257 - 1220 0.208 - 1240 0.124 - 1244 0.305 - 1258 0.278 - 1264 0.443 - 1311 0.232 - 1336 0.258 - 1340 0.237 - 1389 0.045 - 1421 0.063 - 1439 0.045 -

Explain the output -- Processing locus 502: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAGTAAAAAACTAAACAATATTATAGATCATACATCATGATCATATTCGCAGATTCTTGAGACAATAATACTCTGGTT TCATTTACCTTCCCACTTTGAGTAACAATGGCAGCAATCATCGGTGACTGCTTCATATTTATCCTCTTATGCCTCTTCTC ACTCCTTTTCTACTATCTCATCTTCAGGAAACCTAAGAACTCACGGCTTGTCGCTTATGGCTGTGATCTGCCTCCGAGCC CTCCATCTCTTCCGATTATAGGCCATGTTCACCTTCTCCTCTCTTCCCTAGCTCATAAATCTTTACAGAAACTCTCATCC AAGTATGGACCTCTTCTCTATCTCCGCGTCTTCAACTTCCCTGTAGTCCTCGTCTCCTCGGCGTCTGTGGCCTACGAGAT CTTTAAGGCTCATGACTTGAACATCTCATCTCGTGACAACCCTTCAATCGATGATTCCCTCTTGATTGGATCTTCTGTCT TTGGTAGTGCTCCTTATGGAGATTACTTTAAGTTCATGAAGAAGCTCCTGGTTACTAAGCTGCTTGGACCACAGGCACTT GAGCGGTCAAGAGGCATCCGTGCAGATGAGCTTGAGCGGTTTCACTCAAGCCTGCTTGATAAGGCGGTAAAGAAGGAGAT CGTTGAGATTGGGAAGGAAGCAACGAAGCTTAGTAATAACAGCCTCTGGAGGATGAGCATTGGGAGAAGTTTTTCAGAGA AGAATGGTGAGGCTGAGAGAGTCAGGGGACTGGTTACTGAGTTAGATGGCTTGACAAAGAAGGTCTTGTTTGCAACTTTG CTGCACAGGCCCCTTGAGAAGCTTGGAATCTCTCTGTTCAAAAAGGAGATCATGTGTGTTTCTGACAGCTTCGATGAGGT CCTTGAGAGGGTTCTTGTGGAACATGAACAGAAACTGGACGATCATCAAGATAGGGACATGATGGACGTGTTGTTGGCAG CTTATGGAGATGAAAACGCAGAGCATAAAATCAGCAGGAACCACATCAAAGCATTTTTCGTGGAGCTTTTCTTTGCGGGT ACTGATACCTCGGCGCAATCAATACAGTGGACAATGGCAGAGATCATTAACAACCCCAACATTCTTAAAAGATTGAGAGA AGAAATCGATTCTGTGGTAGGGAAAACAAGGTTGATTCAAGAAACTGATCTACCAAAGCTCCCCTATTTGCAAGCTGTGG TTAAGGAAGGACTTAGATTGCACCCGCCATTGCCTCTCTTTGTAAGGACGTTCCAAGAAGGGTGTAAGATTGGAGGTTTC TATGTACCAGAAAAGACAACACTTATTGGTAATGCTTATGTTATGATGAGAGATCCGAGTGTCTGGGAAGACCCTGAGGA GTTTAAACCTGAGAGGTTTCTATCTTCTTCAAGATCAACGCAAGAAGAGGAAAGAAGAGAGCAAGCACTTAAGTACATTC CTTTCGGTAGTGGACGGAGAGGCTGTCCTGGATCAAGTCTGGGTTATATCTTTGTTGGCACCGCAGTTGGAATGATGGTG CAATGCTTTGACTGGAGTATCAAAGGAGACAAGGTCCAAATGGATGAGGCTGGAGGACTAAACTTGAGTATGGCTCATTC CCTGAAGTGCACTCCTGTTCCTCGAAACCGGCCTTTACTTGCTGATCAGTAGATGCCAAGTTTGTAATTTTCAGATGTCA CTGAACTCGCAAACATAGAAAAGTGCTGAATTCAGATGTTTACTACTGGTATGTTTTAATTCCGTTTACTG ......................................N......................................... ...........................i........................................N........... ........................................................N....................... ........................N....................................................... ....N........................................................................... ...........N......................................N............................. ...............N...................N............................................ .........................N...................................................... ....................................................N........................... ...N.........................................N.................................. ...................................................i.....................N...... .......................N..................................i..N.................. ...N.....N...................................................................... .................................i.............................................. ................................................................................ ................................................................................ ................................................................................ .......................................................................i..i..... ..N....................................i...N.........................i.......... ....................................................N.....................N..... ...................................N..............N.................... Pos Score Pred ------------------------ 39 0.286 - 108 0.670 Yes 149 0.344 - 217 0.375 - 265 0.294 - 325 0.476 - 412 0.255 - 451 0.429 - 496 0.223 - 516 0.448 - 586 0.258 - 693 0.342 - 724 0.252 - 766 0.167 - 852 0.736 Yes 874 0.124 - 904 0.480 - 939 0.607 Yes 942 0.424 - 964 0.154 - 970 0.099 - 1074 0.816 Yes 1282 0.249 - 1312 0.456 - 1318 0.306 - 1323 0.412 - 1326 0.545 Yes 1512 0.692 Yes 1515 0.564 Yes 1523 0.353 - 1560 0.841 Yes 1564 0.150 - 1590 0.740 Yes 1653 0.258 - 1675 0.089 - 1716 0.078 - 1731 0.048 -

Explain the output -- Processing locus 503: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAGCCTTATATTATATCTTAAACTTTGCAAGAATCACTTTGGCTTGTAATGAAATGAAATCCACTGTTTTCATATTCAAA GTGATTCTAGTCTTGTATTAACTATTAAATATTCCAATCTTTAACAACAAAGAAAACTATAATATTCAAAGCCATGTATA TAATAATGGACATGGTCCAATAATTTGGCAATTTAAAAAAATATTAAAGAGAAAAGTAGATTCAAATAAAGTGGTCTATA TTTCAAATTGCAAAATGTGTTGGATCTCAATGAGCCAGTCAAGATTCATTATAAAGTCTTTATTTAGCACAGCAGGAGGT TTCTTACTTGGATCTGCTCTCTCAAATCCGCCGTCTCTAGCCACCGCGTTTTCTTCTTCTTCCTCGTCCTCCTCCGCCGC CGCCGCCGTCGACATGGAAACTCACAAAACCAAGGTTTGCATCGTCGGAAGTGGACCAGCAGCACACACGGCGGCGATCT ATGCATCGAGAGCGGAGCTTAAGCCTCTTCTCTTCGAAGGATGGATGGCTAACGACATCGCTCCCGGCGGTCAATTAACT ACAACAACCGACGTCGAAAACTTCCCTGGGTTCCCTGAAGGTATTCTCGGTATTGATATCGTTGAGAAATTCAGAAAACA ATCGGAGAGATTTGGAACTACGATCTTCACGGAAACTGTTAACAAAGTTGATTTCTCATCGAAACCGTTTAAGCTATTCA CTGATTCGAGAACTGTTCTCGCTGATTCTGTAATCATTTCTACTGGAGCTGTTGCTAAACGTCTTAGCTTCACTGGATCT GGTGAAGGTAATGGTGGTTTTTGGAATCGTGGTATCTCCGCTTGTGCTGTTTGCGACGGAGCTGCTCCGATTTTTAGGAA TAAGCCTCTTGTGGTTATTGGTGGTGGTGATTCAGCTATGGAGGAAGCGAATTTTCTGACTAAGTATGGATCTAAGGTTT ATATTATTCATAGGAGGGATACGTTTAGGGCGTCTAAGATTATGCAGCAGAGAGCTTTGTCTAACCCTAAGATTGAAGTG ATTTGGAACTCTGCCGTGGTTGAGGCGTATGGTGATGAAAATGGACGTGTTCTTGGAGGATTGAAGGTGAAGAATGTTGT TACTGGGGATGTTTCAGATCTGAAGGTGTCTGGATTGTTCTTTGCTATTGGTCATGAGCCAGCTACGAAGTTTTTGGATG GGCAGCTTGAGCTTGATGAAGATGGTTATGTTGTGACCAAGCCAGGTACTACTAAGACGAGCGTGGTTGGTGTATTTGCT GCTGGAGATGTTCAAGACAAGAAGTATAGACAGGCCATCACTGCTGCAGGAACTGGGTGCATGGCGGCATTGGATGCAGA GCATTACTTACAAGAGATTGGATCTCAGGAGGGTAAGAGTGATTGAAGAAGCTGTAGGAGAAGGATCCATGATAAAATCA CTCTCTCTCCACCTTATTTGCTTTGCCATTTTTATAACCAATAAAGGATCAGATTGATCTCATAC ................................................N....N.......................... .........................................................................i...... .....N.....N.................................................................... ..............N..............i.................................................. ................................................................................ .............i.................................................................. N.......................................N...i................................... ................................................................................ ................................................................................ ................................................................................ ..........N..................................................................... .....................................i...........................N.............. .........................................i...................................... ............................N.....N.....N................................N...... ........N............................................N.......................N.. ...............N.....N.....N.................................................... .......N....................................................i............N...... ....................................................................N........... ................................................................. Pos Score Pred ------------------------ 49 0.155 - 54 0.487 - 154 0.631 Yes 166 0.372 - 172 0.143 - 255 0.446 - 270 0.501 Yes 414 0.759 Yes 481 0.242 - 521 0.314 - 525 0.798 Yes 811 0.228 - 918 0.700 Yes 946 0.153 - 1002 0.609 Yes 1069 0.082 - 1075 0.111 - 1081 0.157 - 1114 0.200 - 1129 0.093 - 1174 0.103 - 1198 0.144 - 1216 0.125 - 1222 0.133 - 1228 0.245 - 1288 0.345 - 1341 0.685 Yes 1354 0.149 - 1429 0.077 -

Explain the output -- Processing locus 504: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGCAAGATTTACGCAAACATTTTTGTCAACACGTTCTCCTACTATGAAGCTACCAACTTGATAACTCTAACTTCATCCTC AAATCCATCGTTCATAAATAGTCCAAATTTCGCAAATGCAAAACCAAACAAAAAAATATGCAATCAAGAATGGCCTCAAC TACTATAATCCTTTTCCTCTCATTCAGCATCATTCCATTACTCACCATCGTTAGAGCAGATAACCATTCCGTTTACTGCC CTCCACCACCACCTTGTATTTGTATTTGTAATCCGGGACCACCTCCGCCGCAACCCGATCCCCAACCGCCAACTCCACCA ACATTTCAACCAGCACCGCCAGCCAATGACCAACCACCGCCTCCACCACAGAGCACATCACCACCTCCAGTAGCGACCAC ACCACCAGCACTTCCTCCAAAACCCTTACCGCCACCGTTGTCTCCTCCGCAAACCACACCACCTCCTCCACCAGCTATTA CACCACCACCTCCACCAGCTATTACACCTCCACTATCACCTCCTCCACCAGCTATTACACCACCACCTCCACTAGCGACC ACACCACCGGCACTTCCTCCAAAACCCTTACCGCCACCGTTGTCTCCTCCACAAACCACGCCACCTCCTCCACCAGCAAT CACACCACCACTATCACCTCCTCTGGTTGGAATATGTTCCAAGAATGATACCGAACTAAAGATATGTGCCGGAATTCTAG CAATTAGTGACGGTCTTCTAACCACTGGAAGAGCAGAACCATGTTGTTCCATCATCCGAAATGTGTCTGATCTTGATGCA GTTACTTGTTTTTGTAAATCGGTAGGAGCACCACGTTTTTCCCTCTCTCCCAATTTCGGCATCTTCTTTAAAGTTTGTGG TCGTAGGATTCCACAAGGCTTTAGCTGTCCCGGTCCATCACCAACAATCTCACCACCACCACTTCCTCCACAAACTTTAA AACCGCCTCCGCCACAAACAACACCACCTCCTCCACCAGCTATTACACCACCACTATCACCTCCTCTAGTTGGCATATGC TCCAAGAATGATACCGAACTAAAGATATGTGCCGGAATTCTAGCAATTAGTGACGGCCTTCTAACCACTGGTAGGGCAGA GCCATGTTGTTCCATCGTACGTAATGTGTCTGATCTTGATGCAGTTACTTGTTTTTGTAAGTCGGTAGGAGCAAGACGTT TTTCCCTCTCTCCCAATTTTGGCATCTTCTTTAAGGTTTGTGGTCGCAGGATTCCACAAGGCTTTAGCTGCCCATGATGA CCATTTTCTTGCACCACACACATTCTCCTCATTCTCTCTAGTCTTAAATATACAAAGACAGCCTTGTGATTTTTTTTGGG TTCCAACAATGTAATA ...........................................N.................................... ...................................i.....................i...........i.......... ................................................................................ ................................................................................ .........................i...................................................... ................................................................................ ................................................................................ ................................................................................ .................................N..........i..................N................ ........................................N...................N..............N.... ................................................................................ ................................................................................ ............................................................................N... .......N..................N..................................................... ...N...................N..............N......................................... .........................................................................N..N... ................................................................................ ........N....... Pos Score Pred ------------------------ 44 0.368 - 116 0.517 Yes 138 0.658 Yes 150 0.788 Yes 346 0.535 Yes 674 0.424 - 685 0.534 Yes 704 0.314 - 761 0.147 - 781 0.296 - 796 0.260 - 1037 0.357 - 1048 0.433 - 1067 0.373 - 1124 0.130 - 1144 0.229 - 1159 0.217 - 1274 0.068 - 1277 0.139 - 1369 0.050 -

Explain the output -- Processing locus 505: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGATTCTCAGGTTTGTTTCTTGTCTTAAAAGAAACACAAAATGGGTTTATTTGAATCTTTTAATCATAAAAATACTTAA AGAAAGAGGATCAAATGTTGATTTAGATCTCTAATGAAAGTTTGTGTAACGTATAAACAGCTTATGGAATTGGAGCAAAT CTGCATTTTGAAACCCGATGATGAGGAAGAAATGGAGTCACCATCACCATCGAAAACAGAGGAAAATTTAGGCGTGGTTC CTCTGTCTTGCGCGTTTACGAGACAAGAACATTGCGTTTCCGAGACGAAAGAACGCGAAGAATCGACTAGAAGACTAAGC AGCCTTATTTTTCTTTACCTCATCGTTATGTCCGTACAGATTGTGGGAGGTTTTAAGGCGAATAGTCTTGCGGTTATGAC AGATGCTGCGCATTTGTTGTCGGATGTGGCGGGTTTGTGTGTCTCTTTGTTGGCGATCAAGGTCTCGAGCTGGGAAGCTA ATCCGCGAAACTCGTTCGGGTTTAAACGGCTTGAAGTTTTAGCGGCGTTCTTGTCTGTTCAGCTCATATGGCTTGTCTCT GGAGTGATAATCCATGAAGCCATTCAAAGACTACTTAGCAGAAGCAGAGAGGTTAATGGAGAAATCATGTTTGGGATTTC GGCTTTCGGGTTTTTCATGAACTTGGTTATGGTCCTTTGGTTAGGACATAACCATAGCCACCACCACCACGACCATCATC ACCATCATCATAATCATAAACATCAACATCAACATCATCACAAGGAGGTGGTGGCAGAAGAGGAGGAGGAGGAAATGAAT CCTTTAAAAGGTGAGAAATCGTCTTCAAAGGAGATGAACATTAACATACAAGGAGCTTATCTTCACGCAATGGCTGATAT GATTCAGTCACTAGGTGTTATGATTGGTGGAGGTATCATTTGGGTAAAACCAAAATGGGTTTTGGTTGATTTGATTTGCA CTCTTGTTTTCTCTGCTTTTGCTCTTGCTGCTACTCTACCAATTCTCAAGAACATATTTGGAATACTGATGGAACGCGTA CCGCGTGATATGGACATAGAGAAGCTCGAAAGAGGACTTAAGCGAATCGATGGAGTTAAGATTGTTTATGATCTCCATGT ATGGGAGATAACAGTAGGGAGAATCGTATTGTCCTGTCATATCTTACCAGAGCCTGGAGCTAGTCCTAAAGAGATCATTA CTGGTGTTAGAAATTTCTGTAGGAAGTCTTATGGGATTTATCATGCAACTGTTCAGGTTGAATCAGAGTAGCACAAAAGA GAGTTTCTTCTGTATTATTTGCTTAAGAACATCTTTAGGTAGAACAAGAAAGAAAGTTCAAATTTTCGTGTTTCAATATA GCAAACATTA .........................................N...................................... ..............i..................N.............................N................ .................N..N..........N................................................ ................................................................................ ...........................N...............................................N.... ..N....................N........................................................ ...................................................................N............ .............N.........................................i..........i............. ................N...........N................................................... ..........................................................................i..... .................................i...................................N........N. ...................N..................................i......................... ....................................................................i........... .........N.......................................N.................N........N... N............................................................................... ..............................N...........N..................................... ................................................................................ .......... Pos Score Pred ------------------------ 42 0.434 - 95 0.536 Yes 114 0.245 - 144 0.469 - 178 0.358 - 181 0.380 - 192 0.475 - 348 0.292 - 396 0.447 - 403 0.186 - 424 0.148 - 548 0.460 - 574 0.183 - 616 0.677 Yes 627 0.532 Yes 657 0.173 - 669 0.304 - 795 0.705 Yes 834 0.504 Yes 870 0.452 - 879 0.191 - 900 0.309 - 935 0.505 Yes 1029 0.740 Yes 1050 0.479 - 1090 0.390 - 1108 0.147 - 1117 0.081 - 1121 0.369 - 1231 0.252 - 1243 0.157 -

Explain the output -- Processing locus 506: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGAAAAAGATTATGTGAATTGTCCTTATCTCAATAACTTCCATGAACTTACCTTCGTTAGCCTCCATCTCTTATGGCTC CCCACATACTCTGTCTCCTAGTTAGCCTCCTCTCACTATTTTGTCTCTTTTGGTAATATTCAACAATTCAAACACGCCAA CTTTGAATATTCCGTAATATTTTTTCCATATTCAACAATTTTGCAAAAACGAAGACTTCTTACTCTCTCAATAGTCTTCA ACTGCCTACCCTTACTTATATTTATGTTAAGATCTCTTAGTCATCATTATATTCATATCCCTAGTTCAGTTTTATCTCCA AAGAACAAAAATCATTCTTTTATTCTTTATTCTTTCAAGCTTCTCTGTTTCTTGTCTAAACAAACACCAAGGAACGTTTG TTTTGTGTTTGATACCCACACAAGGTACTTTTATTTGTTAAAATGTTTGAATGAGACAAATAATTGGTTTGGTATAGCTA ATTTTAATGCGACTACTGTTTGTTTAATTAATGTGTGCAGGGGATTTTCTATATTAAATATACTTTGGAATTATCCTTTC GTTATATTAGTGGTCATGGGTCTTGAAGTTGGGTCCTTATGCTTCAAACTTAAAGACGGAGGCTTGACATCAAGAACCAA CAGTTTCAAGAGAGACGATACTAATAGGCACCAGAATTCTCCTAAGAGTACTATGGAGCGGTCGTTGAGTTTCAACAGCT GGGAGGTTCCTAAAGAGACCAAGACTGATTCAGATTTTGAGGTCTTGGAGACAAAGAAGTCAACGCCTAACACTTTGAAT GGAAGAAACTGTGAGAGAATCCAAATCAAGAAACCTACAGTTACTCCACCAGAGCCATTTGTGTTCTTCTCTCCTAGACC TGTCACCGAGCTTGATGCAGCTGCAACTACGTTACAAAAGGTGTACAAGAGTTACAGGACCAGAAGGAACTTAGCAGATT GTGCGGTCGTTGTTGAGGAGCTCTGGTGGAGGACTTTGGAAGGTGCAGCTTTGGATTTGAGCTCTGTGTCTTTCTTTGGA GAAGAAAAACATGAGACCGCTGTTTCGAAATGGGCACGAGCTAGAAAACGAGCTGCTAAGGTTGGGAAAGGCTTATCTAA AGATGAAAAGGCTCAGAAATTAGCTCTTCAGCATTGGCTTGAAGCTGTAAGTCCCCATAACCTAAATATCTTTGTAACTT CATATCAAAGACAAGTACCCTACTTGACATCCAAAGCTATCATTGAATATACTCTAATGATCCATCTCTTGAAATTACAG ATTGACCCACGTCATCGTTACGGCCACAACTTGCACTTCTATTATGATGTCTGGTCAGCGAGCAAGAGCACACAACCATT CTTTTACTGGTTGGATATAGGAGACGGCAAAGATGTAAATCTTGAGAAACACCCTAGAAGTGTTCTGCAAAAACAATGCA TCAGATACTTAGGACCGATGGAGAGAGAAGCATATGAAGTGATAGTAGAAGATGGGAGACTAATGTATAAACAGGGCATG ACTCTGATCAATTCAACAGAGGAAGCCAAGTCGATTTTTGTACTTAGTACTACTAGAAACTTATACGTAGGGATTAAAAA GAAAGGTCTTTTCCAGCACTCTAGTTTCTTATCTGGAGGTGCCACAACCGCAGCAGGAAGGTTAGTCGCCCGCGATGGGA TCCTTGAGGTACTTGAATAAGAAACTACCTGTCTTTGCTTCTCTTAATATGTTATGTAAACTAAAATGTCTGATTTACCT TCTGTTTCTTCAGGCTATATGGCCATATAGTGGACATTA ............i.............................N..............................N...... ................................................................................ ................................................................................ .......................N........................................................ ................................................................................ ..........................................N.......N............................. ......N.......................N................................................. ...............i......................N......................................... ....................................................i........................... ..............................................................................i. ................................................................................ ..............N................................................................. ................................................................................ ..........N..................N.................................................. ..N............................................................................. ........................................................i....................... ...........................................N..i................................. ................................i..........................................i.... .................N...............N.................N..........N..............i.. ................................................................................ ..........................................................................N..... ................................................N....N...........N.............. ..................N.................... Pos Score Pred ------------------------ 13 0.527 Yes 43 0.167 - 74 0.208 - 264 0.107 - 443 0.482 - 451 0.142 - 487 0.107 - 511 0.104 - 576 0.622 Yes 599 0.196 - 693 0.686 Yes 799 0.500 Yes 895 0.237 - 1051 0.366 - 1070 0.295 - 1123 0.214 - 1257 0.600 Yes 1324 0.331 - 1327 0.536 Yes 1393 0.537 Yes 1436 0.600 Yes 1458 0.304 - 1474 0.197 - 1492 0.245 - 1503 0.381 - 1518 0.531 Yes 1675 0.400 - 1729 0.049 - 1734 0.056 - 1746 0.202 - 1779 0.061 -

Explain the output -- Processing locus 507: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAACAAACCAAGATTTAGCATAAATGTTCAAAATTGAAACTTTGATTCCTAACTTCTTGGAATGATTCATATTGAGCTA GATTTAGATCTGTATGATTGATATGATGGGTTTGGTTTGTTTATGAGTAGAGAGAAAATGGGTGATGAGGCAGAGATCAA GGAACATTTAAAGCCACAAGCTTCATCTGAAACAATAGACAAGAAACATAATGTGAAAGGGAAGAGGTTATGGCAGAAAG TCAAGTATCAATTGGTGGAGTTTCATTCATTGCCTGCTTATTTAAGAGACAATGAGTACATCATTGGTCATTACCGATCC GAATGGCCGATCAAACAGATTCTTCTCAGCATCTTTACCATTCATAATGAGACTTTGAATGTTTGGACGCATTTGATTGG GTTTTTCCTGTTTTTGGCACTCACTATATACACTGCAACGAAAGTACCGAGTGTCGTGGATCTTCATTCGCTTCAACACC GTTTACCCGATTTGTTGAGGAAAACAGATCTCCACAAACTTCATTCTGAGCTCATGTCTCGCCTTCCTTCTAGTCCATCT AGTTGGCATGTGATGGACCTTCTTTATAACTGTTTGCCTGAAAGATTTTCTCATGGCAACTACACTGACATGTGTGTTCT GCATTCTGTGAGGGAAGATCTTGCAAACTTGATAGCTCCTTTGATCTTCAGGCCAATTACTCGATGGCCGTTTTATGCAT TTCTAGGTGGTGCTATATTCTGTCTATTAGCAAGCAGCACGTGCCACCTCCTCTCATGTCACTCCGAGCGAGTCTCCTAC ATAATGCTTAGGCTTGATTACGCCGGCATCGCAGCTCTAATAGCGACTTCCTTCTACCCTCCGGTTTATTACTCCTTCAT GTGTGATCCTTTCTTCTGCAACCTCTACTTAGGATTCATAACCATCTTAGGAATCGCCACTGTGCTTGTTTCTCTCCTCC CGGTTTTCCAAAGCCTGGAGTTTCGGGTGGTGAGGGCGTCTCTGTTCTTTGGAATGGGATTCTCTGGCTTAGCTCCGATT CTTCACAAGCTGATCATCTTTTGGGACCAACCTGAAGCCCTTCACATGACAGGTTATGAGATTTTGATGGGTTTGCTTTA TGGGTTAGGAGCTGTGGTTTATGCAACTAGGATCCCAGAGAGATGGATGCCGGGTAAATTCGATATAGCAGGACATAGCC ATCAGTTGTTTCATGTTCTGGTTGTTGCTGGTGCGTTAACGCACTATAGAGCTGGGCTATGAGAGATATTGAAGGATGTT GAAGATGAAGATTGAAGATTAGATGGAATCTTTGATGTTTTAAATCTTGTTTGTAGTAGCTTCATATAAAAGTTGGATTA ........................N.....................................i................. .............N........N..N................i..............i......N............... ..................................................N..................N.......... ...................................................N............................ ..i...........................................N...........N..................... ................................................................................ .....................................................i.......................... .......N....N.......................................N................N.......... ...............................................................N..........i..... .......................................................N........................ ...N..........................................................................N. ................................................................................ .....................................................N.......................... .............................................i.........N..........N............N ....................N.....................N...i................................. ............N.............................................N................N.... ....N.................N...........N............................................. Pos Score Pred ------------------------ 25 0.252 - 63 0.706 Yes 94 0.254 - 103 0.227 - 106 0.290 - 123 0.588 Yes 138 0.839 Yes 145 0.150 - 211 0.196 - 230 0.307 - 292 0.262 - 323 0.851 Yes 367 0.194 - 379 0.294 - 534 0.661 Yes 568 0.396 - 573 0.435 - 613 0.255 - 630 0.401 - 704 0.463 - 715 0.689 Yes 776 0.228 - 804 0.487 - 879 0.329 - 1014 0.474 - 1086 0.767 Yes 1096 0.170 - 1107 0.224 - 1120 0.406 - 1141 0.095 - 1163 0.341 - 1167 0.609 Yes 1213 0.120 - 1259 0.137 - 1276 0.135 - 1285 0.097 - 1303 0.068 - 1315 0.040 -

Explain the output -- Processing locus 508: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGCTTCAAAGGCCAAAACTAAGCTCTTTATTAAGTAACATGGATTAGTTTCTTCATAAAGATAAAACTAATTATTTAAA CAAAGTCAAAGCTAGCTTTAGATGCGTACTAGAGTGAATAATACAAATAGTAAACGATATTCTGATCAGAAAATGGGATT TGACTTTAGCACTTCAAAATCCAAAGCTAAAAGACAAAAAAGAATAGAGGTTCGATTTGCATCTCCATTAATGGGCATCG ATCTTTCTCTTAAGCTCGAGGCCGAGGAGAAAAAGAAAGAGATAGAAGGATCGAAACATAGCCGTGAGAACAAAGAAGAC GAAGAACATGATGCTAGTGGTGATGAAGATGAACAAATGGTGAAAGAAGACGAAGATGATTCTTCTTCTTTAGGTTTAAG AACCCGAGAAGAAGAAAACGAACGTGAAGAGCTCTTGCAGCTACAGATCCAGATGGAAAGTGTGAAAGAAGAGAATACTA GGTTGAGGAAGCTTGTCGAGCAGACTCTTGAAGATTATCGTCATCTTGAGATGAAATTCCCGGTTATCGATAAAACCAAG AAGATGGATCTTGAAATGTTCCTTGGAGTACAAGGCAAACGATGTGTGGATATAACAAGTAAGGCTCGGAAAAGAGGAGC TGAGAGATCTCCGTCAATGGAAAGAGAAATAGGGCTTTCACTTTCTCTAGAGAAAAAACAGAAACAAGAAGAGAGCAAAG AAGCTGTTCAGTCTCATCACCAAAGATACAATAGTAGCAGCTTAGATATGAATATGCCACGTATCATTTCATCTTCTCAA GGTAATAGAAAGGCCAGGGTGTCCGTGAGGGCGAGATGTGAGACCGCAACAATGAATGATGGATGCCAATGGAGGAAGTA CGGTCAGAAAACCGCGAAAGGGAATCCATGTCCTCGAGCTTATTACCGATGCACCGTGGCTCCAGGATGTCCCGTTAGAA AACAGGTGCAAAGGTGTTTAGAAGACATGTCAATACTGATAACAACCTACGAAGGAACACATAACCATCCACTTCCGGTC GGAGCAACAGCCATGGCTTCCACTGCCTCTACTTCTCCATTCTTGTTACTCGATTCCAGTGACAACCTCTCTCATCCTTC CTATTACCAAACTCCTCAAGCCATAGACTCTTCTTTGATTACATACCCACAAAATAGCAGCTACAACAATCGAACCATAA GAAGCTTGAACTTTGATGGTCCATCTAGAGGAGATCACGTTTCATCTTCTCAAAACCGATTAAATTGGATGATGTAGAGT TTCCTATATCTCTATGCTTGTTCTTTGGTCCCATTATTTGTCATTATGGATTCTTTGCCTTTCTTCTTGTTCTCGTTTCT AACATTTATGTTTCGT .......................................N........................................ .....................N..................................................i....... ......................................................................i......... ................................................................................ .......N..N...........N.....N.......i..................N........................ ....................................................N........................... ..................................................i............................. ...i...........N.........................i...................................... ................i............................................................... ...............................................i.....N.......................... ...................................N...............i...i..i...N.....i........... ...........................N....................N.................N............. ..........................N..................................................... ............i................................................................... ................................................................................ ...............N....................................................N..N........ .............N...............................N.................................. .......N........ Pos Score Pred ------------------------ 40 0.348 - 102 0.450 - 153 0.512 Yes 231 0.698 Yes 328 0.395 - 331 0.448 - 343 0.263 - 349 0.125 - 357 0.829 Yes 376 0.237 - 453 0.472 - 531 0.521 Yes 564 0.561 Yes 576 0.423 - 602 0.558 Yes 657 0.627 Yes 768 0.860 Yes 774 0.446 - 836 0.455 - 852 0.582 Yes 856 0.679 Yes 859 0.697 Yes 863 0.234 - 869 0.509 Yes 908 0.491 - 929 0.413 - 947 0.304 - 987 0.384 - 1053 0.792 Yes 1216 0.271 - 1269 0.048 - 1272 0.243 - 1294 0.075 - 1326 0.087 - 1368 0.036 -

Explain the output -- Processing locus 509: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATATATAAATATGTGCATAATAACAAATCTGAGTATGAATAGTAGGAGGGGTGTATAGGGCTTTCAAGTCTTTTCTTAC AGAAACTATCTATAACTCTAGCAGAGAGCTCATTATAGAACAGAACAGAAACAGAGCTTTCTAAGTCTACAGCACAGAAA TAAACCTCATTTACAGACTGTTTTACTTTCTTTCCTTTTTGAGCATTTCTTCTTTCTGTTTCTTTCCTCTGTTTTCTCTT TCTTTCCCTTTTTGGGTGTCATCTCTTTAAGACAGAAGAAACGTTTCTCAGAGTTTCCTGTGAACTCATCTGTTTGTTCT CTTCTTCCGGTTTCACTTTTTCATGTCCTGCCGTTATTACAACGAGGTTGGTTTAAAGATCAGCTTTTATCATTTTTCCC TGCATGTAACAAGAAATATTTAGGTTTCATGTCTGTTTACAAATATGCTCTGTTTCTAAGTATGCCTCTCACATTTGTCT TTGTTTTGGGTGTTTTTAGGATTGTGTTTGATCCGATGGAAGGATTGGAATCTGTGTACGCTCAAGCTATGTATGGAATG ACACGAGAGAGCAAAATCATGGAGCATCAAGGATCAGATTTGATTTGGGGAGGAAATGAGCTAATGGCTCGAGAACTCTG TTCTTCTTCTTCTTATCACCACCAACTCATTAATCCGAATCTTAGCAGCTGTTTCATGTCTGATCTTGGAGTCTTAGGTG AGATTCAACAGCAGCAACATGTTGGCAACAGAGCTAGCTCGATAGATCCATCATCACTCGATTGTTTGTTATCTGCGACG TCGAATAGCAACAACACCTCGACGGAGGACGATGAAGGAATATCTGTGCTTTTCTCAGATTGTCAGACTCTTTGGAGCTT TGGTGGAGTCTCATCTGCAGAGTCTGAGAACAGAGAGATCACTACTGAGACGACAACAACGATAAAGCCTAAGCCTTTGA AGAGAAACAGAGGAGGAGATGGAGGAACTACTGAGACTACAACAACAACAACAAAACCTAAGTCTTTGAAGAGAAACAGA GGAGACGAGACAGGAAGTCACTTTAGTCTTGTTCATCCTCAAGATGATTCGGAGAAAGGAGGTTTCAAGCTTATATACGA TGAGAATCAATCGAAATCAAAGAAACCAAGAACAGAGAAAGAACGAGGCGGTTCTTCGAACATTAGTTTCCAACATTCAA CTTGTTTGTCTGACAATGTCGAGCCCGATGCTGAGGCGATTGCACAAATGAAGGAGATGATATACAGAGCGGCTGCATTT AGACCGGTGAATTTCGGGTTAGAGATTGTGGAGAAGCCTAAGAGGAAGAACGTCAAGATATCGACGGATCCTCAAACGGT TGCAGCGAGACAGAGAAGGGAGAGGATAAGTGAGAAGATTAGGGTTTTACAAACATTGGTTCCAGGTGGGACGAAGATGG ATACTGCATCAATGCTTGATGAAGCTGCTAATTATCTCAAGTTCCTTAGAGCACAAGTAAAAGCTTTAGAAAACTTGAGA CCCAAGCTTGACCAAACCAATCTCTCTTTCTCTTCTGCTCCTACATCGTTTCCATTATTCCACCCATCTTTTCTTCCATT GCAAAATCCTAATCAAATCCATCATCCAGAGTGTTGACAGATTATAAACTTTTGAGTTTCATCATCATCAACAGAATCAT GGCGTCTTGATTGTTTTAGCAGTTCTCAAGAAAGGCAACTTCTGTGACAAGGGTGG ...........i.......................i............................................ ................................................................................ ................................................................................ ................................................................................ ......................N......................................................... ...N........................i...............N................i.................. ...................................i................................N...N....i.. ..................N....................................N.......i................ .......................................................i........................ ..................i............................................................. ...............................N................................................ ................................................................................ ..................N............................................................. ...........................................N...................................N ................................................................................ ...............N...........N...................i........i....................... ................................................................................ ............................................................................i... ...........N......N............................................................. ................................................................................ ..............................................................................N. ........................................................ Pos Score Pred ------------------------ 12 0.548 Yes 36 0.532 Yes 343 0.141 - 404 0.142 - 429 0.763 Yes 445 0.195 - 462 0.611 Yes 516 0.662 Yes 549 0.247 - 553 0.161 - 558 0.530 Yes 579 0.401 - 616 0.241 - 624 0.631 Yes 696 0.667 Yes 739 0.682 Yes 832 0.466 - 979 0.395 - 1084 0.301 - 1120 0.116 - 1216 0.437 - 1228 0.080 - 1248 0.748 Yes 1257 0.558 Yes 1437 0.533 Yes 1452 0.331 - 1459 0.085 - 1679 0.085 -

Explain the output -- Processing locus 510: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAATATGAGTTTAATTTTAAGAAGAAAAAAACAAGCGAAAATTAATAGCAGTCATATATATATATAGCCATAGCCTTAG TTTGACGTCTCTCTCGTGTAGAGAAAAAGAAGAAGACTTGTTCAAAGAGAGAAAGAAGAGATCACACACAACATGGATCA TCATCAACTCTTCTCTCTCTGCTCATTTTCGTACATCTTCAAGATCAAGAAACATCTCTTCGTCAGCCTCTTTTTACTTA GCCTCCTCATATTCTCGACCGTGGTCGTCGACGTCATGCCCTCTCTTCGTATCGGATTGTTGTCATCGTCATCATCGCAA ACAGTAACAAAGGAGTGCGATTACTCCAAAGGAAAATGGGTTCGTCGAGCATCATCATCATCATCATCCGTTAATGGATT GTTTTATGGCGAAGAGTGTCGCTTTCTTGACTCTGGTTTTCGTTGTCATAAGCATGGAAGAAAAGATTCTGGTTATCTTG ATTGGCGATGGCAACCACACGGCTGCGATCTTCCACGATTTAACGCAAGTGATTTACTTGAGAGGAGTCGGAACGGGAGG ATAGTGTTCGTGGGAGATTCCATCGGAAGAAACCAGTGGGAATCCCTTATGTGCATGCTCTCACAAGCAATACCTAATAA ATCTGAGATCTATGAAGTAAATGGGAACCCTATAACTAAACACAAGGGCTTTCTCTCCATGCGATTCCCTCGGGAAAACC TCACTGTTGAGTATCACAGATCACCCTTTCTTGTCGTGATTGGTCGGCCACCGGATAAATCACCGAAAGAGATCAAAACC ACAGTGAGAGTTGATGAGTTTAACTGGCAGTCGAAAAGATGGGTCGGGTCAGACGTTCTGGTCTTCAATTCAGGGCATTG GTGGAACGAAGACAAAACTGTGTTAACGGGATGCTATTTTGAGGAGGGAAGGAAAGTGAACAAGACAATGGGAGTAATGG AGGCATTTGGAAAGTCTTTGAAGACATGGAAGTCATGGGTCTTGGAGAAACTTGATCCTGACAAGAGTTATGTCTTCTTC AGAAGCTACTCTCCAGTGCACTACAGAAATGGGACATGGAATACCGGTGGTCTATGTGATGCAGAGATTGAGCCTGAGAC TGATAAAAGGAAGCTGGAACCTGATGCGAGTCACAATGAATATATCTATAAAGTAATTGAAGAAATGAGATATCGACATA GTAAGGTTAAGTTTCTGAACATTACATATCTGACAGAGTTTAGGAAAGATGGTCATATTTCTCGGTATCGAGAGCAAGGC ACTTCTGTTGATGTTCCTCAAGACTGTAGCCACTGGTGCCTACCCGGTGTACCAGATACATGGAATGAGATCCTCTATGC GCAACTCTTGTCAATGAACTATAGAACAAAGTGATTCCTTGAGGTAAATACTGATTTTATGTTTCTTGATCTAGTCATGA TTCTGGTTCATCAATGCCTTCTTCAGGCTCAGTTTCATTTGTTTGCATTTGCT .....N.......................................................................... ........................................................................N....... ................................................................................ ...................................N............................................ ...................................N.....................................i...... .....i...............................................i.......................... .......i........................................................................ ................................................N.....N......................... ...........N........N.....................................i..................... ................................................................................ .............N........................i......................................... ..............................N....................................i........N... .........................N........N..................................i.......... ............................N......N.................N....N..................... .......................N...........N............................i............... ................................................i............................... ..........N................................................N....N...........N... .............N............................................N.................N... .............N....................................... Pos Score Pred ------------------------ 6 0.343 - 153 0.168 - 276 0.234 - 356 0.229 - 394 0.574 Yes 406 0.860 Yes 454 0.590 Yes 488 0.714 Yes 609 0.348 - 615 0.200 - 652 0.383 - 661 0.228 - 699 0.571 Yes 814 0.309 - 839 0.722 Yes 911 0.387 - 948 0.501 Yes 957 0.241 - 986 0.397 - 995 0.361 - 1030 0.507 Yes 1069 0.351 - 1076 0.311 - 1094 0.166 - 1099 0.153 - 1144 0.148 - 1156 0.120 - 1185 0.689 Yes 1249 0.540 Yes 1291 0.134 - 1340 0.269 - 1345 0.205 - 1357 0.096 - 1374 0.270 - 1419 0.114 - 1437 0.065 - 1454 0.051 -

Explain the output -- Processing locus 511: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAATGCTGTGAAAATTGTTGTCTGAGTCACATATTTCGTGTAGAAATGACAATGAAGTTTATCTGTGGCTAAAGAATCG ACAATATATATTTTCATTTGATTGATGCAGGGAAGAGAAGATAAGCTAAACAAGAGAATGGTGACAGGTTGTGTTGATCT TCATCAATCTTTCAAATCTGCAGATTCATCTTCTGTGCCAATTCCTCCTCCTCTTCCATCAAAATCTGATGGACTCAAGA AAAAACTTGGGCATAGCAGCGTTTCGACAGCCACAAGAGATATGTGGGATCGTCTTTTCAATGACGGATACAAAGCTGAT GTAGTCATCTATACAGATAATGGCAGTATCATCTATGCTCATGCCAACATCCTTGGAACTGCTTCCACTGTGATCAAAGG CATGTTGAAGCAAGCCAAGAGACATGGCAAGTGGCATACAATATCAATCCGAGGTGTCCCTCATGATGCTGTGCGAGTTT TTATCCGTTTCCTCTACTCTTCTTGCTATGAGAAAGAAGAAATGAACGAGTTTATCATGCATTTGCTACTTCTGTCACAC GCGTACGTGGTTCCTCAGCTGAAACGGGTCTGTGAATGGCATCTAGAACATGGTTTGCTTACCACTGAGAATGTGGTTGA TGTGTTCCAGCTTGCGCTGCTGTGTGATTTCCCTCGGCTTAGCCTCATATCTCACCGTATGATCATGAAACACTTTAACG AACTCTCTGCGACAGAAGCATGGACAGCTATGAAGAAAAGCCATCCCTTTCTTGAGAAAGAAGTTAGAGACTCAGTAATC ATCGAAGCAAATACGCGGAAAGAGAGGATGAGAAAACGCAATGATCAGAGGATATATTCGCAGCTATACGAAGCAATGGA AGCACTTGTTCATATATGCAGAGATGGATGTAAAACAATAGGGCCACACGATAAAGATTTCAAACCGAACCATGCAACGT GTAACTATGAAGCTTGCAAGGGATTAGAATCACTGATCCGGCATTTCGCAGGCTGTAAGCTAAGAGTTCCAGGAGGTTGC GTGCATTGCAAGAGAATGTGGCAACTCCTTGAACTGCATTCACGTGTTTGCGCAGGTTCTGATCAATGTCGAGTCCCTCT ATGCAGAAACCTTAAGGAGAAGATGGAGAAACAGAGCAAGAAAGATGAGTCGAGATGGAAACTTCTGGTGAAGAATGTAT TGGGGAGTAAGAAAATCGGAGGCTCTCCTTTCTTTTTACCGGTGACAAACTGTTAAAAAACATTTTGTCAATCTCAAGAA GGGCGAGTTTTAAGCGTTTCTTGTGATCCTATCTTTAAAACAAGTGTAACATAATGAGTTTGAATAAATGACACA ...N..........................................i.....N........................... ........................i................................i...................... ....................................................................N........... .........................................i..................N.................N. ...................N..............N.....N....................................... ...........................N.............i..............i....................... ...................................i.............N....................N........N ..........................................................N.....i............... ...................i.........i.................................................. ...........................N............N..................................N.... ...............N.......N...N...........................................i........ ......i......................................................................... ...............i.................................................i.............. N.....................N.....................N.........N...................N..... ................................................................................ .....................................................N.............N....... Pos Score Pred ------------------------ 4 0.274 - 47 0.656 Yes 53 0.438 - 105 0.635 Yes 138 0.686 Yes 229 0.470 - 282 0.746 Yes 301 0.344 - 319 0.230 - 340 0.179 - 355 0.262 - 361 0.343 - 402 0.500 - 424 0.647 Yes 463 0.184 - 466 0.288 - 508 0.288 - 522 0.830 Yes 537 0.581 Yes 596 0.634 Yes 610 0.328 - 631 0.311 - 640 0.187 - 699 0.385 - 705 0.528 Yes 740 0.759 Yes 750 0.701 Yes 828 0.341 - 841 0.253 - 876 0.446 - 896 0.264 - 904 0.473 - 908 0.139 - 952 0.664 Yes 967 0.720 Yes 1056 0.593 Yes 1106 0.590 Yes 1121 0.443 - 1143 0.428 - 1165 0.469 - 1175 0.438 - 1195 0.479 - 1334 0.076 - 1348 0.061 -

Explain the output -- Processing locus 512: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTAAAACCTAGTGAATAGAAGAAGAATATACTTTCAAACAACACGGAGTTTATCTTCTATTGGCATACTAGATTTCTCA GAGGAATATTCTTAATGCTGATTGGGTTGTTACGATTTTTTGGCTAAATCTTCTAAAGATAAGTTGCAGGTCTATCTATA TAATGTTGGATTCATGCAGTGTGGTCGTTGGAGGAATTTTTCTAGCTCAAATTCAGTCATGGCCTTCAAGTATCTTCTAC CATTGCTTTTACTGTCATTGTTGGTTGCTAATTGTGCCTCAAGGCCTTTGTATCGTCTCCCAAGCGAAGCAAAACATGCA ACCAAGAAACCACTTCAGACTTCCCGTCCTTACAACCTTGCACACAGAGGATCAAACGGAGAGCTCCCTGAAGAAACTGC TCCTGCATATATGAGGGCCATAGAGGAGGGTGCAGATTTTATAGAAACCGATATTTTGTCTTCCAAAGACGGTGTTCTTA TATGTCACCACGATGTTAATCTCGATGATACAACCGATGTTGCAGACCATAAGGAGTTTGCAGACCGTAAGAGGACTTAT GAAGTCCAAGGAATGAACATGACCGGCTTCTTCACTGTTGATTTTACTCTCAAAGAGCTGAAAACACTTGGTGCAAAGCA GAGGTATCCTTTCAGGGATCAACAATACAATGGTAAATTCCCGATTATTACCTTCGACGAGTATATTTCGATAGCACTGG ATGCACCTAGAGTGGTTGGGATATATCCAGAGATCAAGAATCCGGTTTTCATGAATCAGCAAGTGAAGTGGGCGGATGGT AAAAAATTTGAAGATAAATTTGTGGAAACTTTGAAGAAATATGGATACAAAGGCTCATATCTGTCTGAAGATTGGTTAAA ACAGCCAATATTTATTCAGTCTTTTGCAGCAACTTCGCTAGTTTACATTTCAAACATGACAGACTCACCAAAATTGTTTT TGATCGACGATGTCACTATTCTTACCGAAGACACTAATAAGACTTACGCAGAGATCACATCAGACGCATATTTGGATTAC ATTAAACCATATGTAATTGGGATTGGTCCATGGAAAGACACAATTGTTCCTGTGAACAATAACCGTCTCATGACACCAAC AGATTTAGTTGCGAGAGCACATTCGCGTAATCTTCAGGTCCATCCATACACATACCGTAATGAGAACCAGTTTCTGCATT TAGAGTTTAATCAGGACCCTTACCTAGAGTATGATTATTGGCTCAATAAGATCGGTGTTGATGGTTTGTTCACTGATTTC ACTGGAAGTCTACACAATTACCAGGAATTGAAGTCTCCTTTGCCTCAGCAACAGTGATTCTCCAATTGAATTTGTTCTTT TCTTTCTTATGCTAGTTTATGTTCTCTTAGGATTTTTTTTTACAAACCCATGGATGTTTCCATTTTCTTCGATTTC ................................................................................ ..............N................................................................. ..N..........N............................................N..................... ...........................................................................N.... ................................................................................ ..........i..................................................................... ............i.....i............................................................. .............................N.................................................. N.................................................N........................N.... ........................................N....................................... .......................................................i........................ .........N...................................................................... ..........N..................N.......................................N.......... ...........................................................N.................... ..............................N.............................N................... ................................................................................ ........N.........N..............................N...N...................... Pos Score Pred ------------------------ 95 0.465 - 163 0.226 - 174 0.078 - 219 0.493 - 316 0.451 - 411 0.704 Yes 482 0.364 - 493 0.159 - 505 0.075 - 517 0.202 - 559 0.148 - 573 0.542 Yes 579 0.758 Yes 670 0.347 - 721 0.103 - 771 0.287 - 796 0.237 - 841 0.383 - 936 0.775 Yes 970 0.360 - 1051 0.111 - 1070 0.348 - 1110 0.131 - 1180 0.134 - 1231 0.333 - 1261 0.281 - 1369 0.060 - 1379 0.054 - 1410 0.078 - 1414 0.056 -

Explain the output -- Processing locus 513: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGCAATTAGTAGTAACAAATGAAAAATGCAATATTGAGCTAATATCGAGTGACTCTGCTTCTTGTTACCATACACGTAT GTAATCTTTCATGATCTTTAGATTCACGGGTCCCTTGAGAGATCTTTTACAGTTGAAAAAAGCTTCTCTCTTCACATGGA TTTAGTGTCTTCAAGAGATACTTTAGGAGATCCTTCAACTTCAACAAAAGCCCTTCTTCTTGGTTTCTCTCGTTCTCGGT CTGATCATTGCATCATCATGTCTGGAGAATCAAGCTCTGGTTCTACCGAACATTGCATCAAAGTTGTACCGACACACGGT GGCCGCTATGTTCAGTACAACGTTTATGGACAACTCTTTGAAGTTTCCAGAAAGTATGTCCCTCCTATTCGTCCCATTGG TAGAGGCGCTTGCGGTATTGTCTGTGCTGCGGTGAACTCAGTGACTGGAGAGAAAGTGGCTATTAAGAAGATCGGTAATG CTTTTGATAACATCATCGATGCTAAGAGAACGCTACGTGAAATTAAACTTCTCAGGCATATGGATCATGAGAACGTTATA ACCATCAAAGATATTGTAAGACCTCCGCAACGAGATATCTTCAATGATGTCTACATTGTCTATGAGTTAATGGACACTGA TCTTCAGCGAATCCTCCGTTCTAACCAAACACTGACCAGTGATCAATGCCGTTTCCTAGTATACCAGCTCTTAAGAGGGC TCAAATACGTGCACTCGGCCAACATATTACATCGTGATCTAAGGCCAAGCAACGTGCTACTTAACTCGAAAAACGAGCTA AAGATTGGTGATTTTGGGCTTGCAAGAACAACTTCCGACACAGACTTCATGACTGAATATGTCGTTACCCGTTGGTACAG GGCTCCTGAGTTGCTTCTTAACTGCTCAGAGTACACCGCAGCTATTGATATTTGGTCTGTTGGCTGCATACTCGGCGAAA TCATGACGGGACAACCGTTGTTTCCAGGCAAAGATTATGTTCATCAGCTTAGGCTTATAACAGAGCTTGTAGGCTCTCCA GACAATTCCAGCCTCGGCTTCCTTCGCAGTGACAACGCAAGAAGATACGTCAGGCAACTTCCGCGATACCCGAAACAACA GTTTGCTGCTAGATTCCCGAAAATGCCCACTACGGCTATCGATTTGCTTGAGAGAATGCTCGTCTTTGATCCTAACCGGC GCATCTCAGTCGATGAAGCCCTTGGCCATGCTTACCTATCACCGCACCATGATGTGGCCAAAGAACCGGTCTGTTCGACT CCTTTCAGCTTTGATTTCGAACATCCTTCTTGCACAGAAGAACACATAAAGGAGCTTATCTACAAGGAGTCTGTCAAATT CAATCCTGACCACTGAGAGAGAAACATGTTTGAATGATTTGATTCCTTTTTAAAAGTCTGCTCCGTTTGGTTTGATCTAA GATTCCTTTATATTGAATCTAAACTCATTTTATCT ...................i......i...................................................N. ..........N................................................................i.... ................................................................................ .................i.............................................................. .......N.................N.............................N........................ .............................................................................N.. ..................N........................................N......N............. ...........................................i..i..............N.......N.......... .............................................i.................................. ................................................................................ ................................................i.........i..................... ................................................................................ ..i.................................i........................................... ................................................................................ ......................i................................i........................ ............N..............N....................N..N............................ ................................................................................ .........................N.......N.............................................. ................................... Pos Score Pred ------------------------ 20 0.585 Yes 27 0.535 Yes 79 0.120 - 91 0.159 - 156 0.655 Yes 258 0.696 Yes 328 0.358 - 346 0.306 - 376 0.123 - 478 0.190 - 499 0.131 - 540 0.430 - 547 0.126 - 604 0.503 Yes 607 0.629 Yes 622 0.360 - 630 0.379 - 686 0.761 Yes 849 0.644 Yes 859 0.537 Yes 963 0.685 Yes 997 0.573 Yes 1143 0.845 Yes 1176 0.676 Yes 1213 0.305 - 1228 0.191 - 1249 0.184 - 1252 0.339 - 1386 0.149 - 1394 0.091 -

Explain the output -- Processing locus 514: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTGTAAAAAAAAAAAATCGATTTTACAAACAAAAAAATATACTTTATGAATGTGGTATTCATTTTAAATTTTTCGCCA AAATATTATTTATTTTGATAGCCAAAACGGCAAAACCCAAAAGGCCCAAAACGAATCTTAAATATGGTCCGGGTCATGGA TAATACAAAACGGGTCAGGCCTAATCATCTCTTTAAGGCCCAATATGGTTTAGTAAGGAAAGGACTCTCAATTTGGCGGG AGAGAGAAAGAGAAGCTTCTCTAATCTCTTCACCGTCTAAAGAATATTTCCGACGCCGGAGACGAAAACAAAAAATGGCA AACAAACTCATCGGAGAAATGGGTCTTCACACCAAAATCTCAAACATCTTCGCTGCAAGAAACATCATCACCGCCAAGGA TGCATTATCAATGACGGAATTTGAACTAATGGAGCTGTTAGATGTTGGAATGAAAGAGATAAGATCAGCAATTTCATTCA TCAGTGAAGCTACTTCTCCACCATGTCAATCTGCTCGATCTTTACTGGAGAAGAAGGTCGAAAACGAACATTTATCAGGT CATCTTCCTACACATTTGAAGGGGTTAGATGATACCTTGTGTGGTGGGATACCTTTTGGTGTTCTTACTGAGTTAGTTGG TCCTCCTGGTATTGGTAAATCACAGTTTTGCATGAAACTTGCGTTATCAGCTTCGTTTCCAGTAGCTTATGGAGGATTAG ATGGTCGTGTGATATACATAGATGTGGAATCCAAGTTTAGTTCAAGAAGGGTGATAGAGATGGGACTGGAAAGCTTTCCG GAAGTGTTTCATCTTAAAGGAATGGCACAAGAGATGGCTGGAAGAATCCTTGTTTTGCGTCCAACATCTTTAGCTAACTT TACTGAAAGTATACAAGAACTCAAGAATTCAATTCTTCAAAACCAAGTAAAGCTTCTAGTGATTGATAGTATGACAGCTC TTCTTTCAGGCGAAAACAAACCAGGAGCTCAGAGACAACCTCAGTTGGGTTGGCATATCTCTTTCTTAAAATCGCTTGCT GAATTTTCACGGATTCCTATAGTGGTGACTAATCAAGTTAGATCTCAAAACCGCGATGAAACTAGTCAGTATTCTTTCCA AGCTAAAGTTAAAGATGAATTCAAAGACAACACAAAGACATATGATTCTCACCTTGTTGCTGCATTGGGGATTAACTGGG CTCATGCTGTAACCATCCGACTGGTCCTTGAAGCCAAGTCAGGTCAGAGAATCATTAAGGTGGCAAAATCTCCTATGTCG CCTCCTTTAGCCTTCCCGTTCCATATAACATCAGCTGGGATTTCATTGCTGAGCGACAACGGGACTGAACTGAAAGGTCC AGGAATCAACACCATTCATGCTCGAGGTATAGTTGATCCCGATTATGTCGCTTTATTTCGCACATTTTAGCATGAATCCA ACTTGAGCTGATAAAGAGATTCTGTTGAAATAAGTCATTAGCCTTCTGATGCATTTGATAACCTTTTGAGGTAGAGTGAA TATTACAGT ...............................................N...i............................ ...............................................................i...........i.... ............................................i................................... ..........................................................................i..... ..................i............................................................N ..........i.................i............N.......i.............................. ......................N......................................................... ............................N................................................... ...............................N....................................N........... N....................N.....................................N.................... .....................N...........N.............................................. ......................................................................N......... ................................................................................ .......................................................N........................ ..............N..........................N...................................... ...N......................................................................N..... ................................................................................ .................N..........................N..........................N........ ................................................N............................... ......... Pos Score Pred ------------------------ 48 0.178 - 52 0.644 Yes 144 0.690 Yes 156 0.595 Yes 205 0.762 Yes 315 0.710 Yes 339 0.540 Yes 400 0.197 - 411 0.540 Yes 429 0.523 Yes 442 0.408 - 450 0.663 Yes 503 0.291 - 589 0.319 - 672 0.408 - 709 0.123 - 721 0.298 - 742 0.287 - 780 0.168 - 822 0.301 - 834 0.360 - 951 0.384 - 1096 0.190 - 1135 0.412 - 1162 0.223 - 1204 0.106 - 1275 0.475 - 1378 0.488 - 1405 0.248 - 1432 0.208 - 1489 0.037 -

Explain the output -- Processing locus 515: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACTAGATAGTAAGGGAATTGATTCAATGGTTCATCTCCTTTGCATAAAGTTATATTCTGTGTATTAGTGTCTCATTTCT CGTTGCCATTCAACACTCTGAATTAGGATACTGTGTGGTGTTTTGCAAAACTGCTCTTGTGCTATGACTCTGGCTAATGA TTTCGGATATTCGACCGCTATGTCTTCATCTTATTCAGCTCTTCATACTAGTGTAGAAGACAGATACCACAAGTTGCCTA ACTCTTTTTGGGTTTCATCAGGTCAGGAACTGATGAATAACCCTGTACCGTGTCAGTCGGTCTCTGGTGGCAATTCTGGT GGTTATTTGTTTCCTTCTTCCTCTGGATACTGCAATGTTTCAGCTGTCTTGCCTCATGGAAGGAATTTGCAAAACCAGCC ACCTGTTTCCACCGTGCCTAGAGACAGATTGGCTATGCAGGATTGTCCTTTGATAGCTCAATCCTCGTTGATTAATCATC ACCCTCAAGAGTTCATAGATCCACTTCATGAGTTCTTTGATTTCTCTGATCATGTTCCTGTTCAGAATCTACAAGCAGAG AGTAGTGGAGTAAGGGTGGACTCATCCGTGGAACTTCATAAGAAAAGTGAATGGCAAGATTGGGCAGATCAGCTGATTTC TGTTGACGATGGTTCTGAGCCAAATTGGTCTGAACTTCTTGGAGATTCAAGTTCCCACAATCCAAATTCAGAGATACCTA CACCATTTTTGGATGTACCAAGGCTAGATATAACAGCTAACCAGCAGCAACAGATGGTTTCATCAGAGGACCAGCTCAGC GGCAGAAACTCATCATCTAGTGTAGCAACATCTAAACAACGTATGCGTTGGACACCAGAACTTCACGAGGCATTCGTTGA AGCTGTTAATCAGCTTGGTGGTAGTGAACGAGCCACCCCTAAGGCTGTTTTGAAGCTCTTGAATAACCCTGGCTTGACCA TTTATCATGTAAAAAGCCATTTGCAGAAATATAGAACGGCAAGGTATAAACCGGAGACTTCGGAAGTTACAGGAGAACCT CAAGAGAAGAAGATGACATCTATTGAAGATATCAAATCTCTTGACATGAAAACGAGCGTTGAGATCACACAAGCTCTACG GTTACAAATGGAAGTGCAGAAACGTCTCCACGAGCAGCTCGAGATCCAACGGTCACTGCAGTTACAGATTGAAAAACAGG GCCGGTACCTACAGATGATGTTCGAGAAACAACAAAAGATACAAGACAACAAGAGCTCTTCTTCAGAAGCATCACCAAAG CAATGCAATGGTTCATTTGCAGAAGTTGAAGTTGGTCTTGAGACACTAACAGGGGACCAAAATGAATCTGCTTCAGCATC AAGGAAACGTGTCAGAGAAGATTAAAGCATGTTTAATTTGATCTCGTTTGACGAAACATGTTTATTGGGAATTAATTGTG GAAACCAACTCCAGTTTTGTTAAAAGAATCTGTTTCTTTCTTAG ..........................N..................................................... ...............................................................i............N... ...................i............................................................ ................................i............................................... ..................................N....................N........................ ..................................N............................................. ...........................N.......................N............................ ..................................................i............................. ........N....................................................................... ............N........................................i.......................... ..........................................i..................................... ................................................................................ ......N......................................................................... ............i................................N.................................. .......i........................................................................ ..............N..i.............................................................. ..N....i.....................................................N.................. ............................N............................N...................... ............................................ Pos Score Pred ------------------------ 27 0.374 - 144 0.719 Yes 157 0.242 - 180 0.502 Yes 273 0.642 Yes 355 0.184 - 376 0.165 - 435 0.472 - 508 0.175 - 532 0.284 - 611 0.546 Yes 649 0.215 - 733 0.386 - 774 0.529 Yes 843 0.537 Yes 967 0.273 - 1053 0.685 Yes 1086 0.335 - 1128 0.579 Yes 1215 0.149 - 1218 0.519 Yes 1283 0.191 - 1288 0.633 Yes 1342 0.258 - 1389 0.203 - 1418 0.101 -

Explain the output -- Processing locus 516: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 517: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCTGAGACCCTCACGAGACTGCATGCATGAGACACTTGTGAATATAGCCGCAGCTAAAAAGAAGTTTGGTAAGAGAGAG AAAGATAGAGAATTAAAATAGAAAAACCAACTGTCACAAAATTATCAACACATGAAACCAATTATAACATACCGAACCAA ACCAACCAACTACATTTCCTCATTATATAACTATGAACTCCACTCAAATCAATTTTCAATCTCATTCTTCTTAAAAACCA TTACAATTATTTCACTAAATTATAACTTAATGGGCATTCTCCGTTTTGTATTGTTGATTAGTTTGAATCTGGTTTTGTTT GGTTTTAAAACGACCGTGTCACAGCCACAACAACAGGCACAAGTCCCATGTTTATTCATCTTCGGTGACTCTTTAGTCGA CAATGGAAACAACAATAGGTTGCTTTCTCTTGCGAGGGCTAATTACCGACCTTATGGCATTGACTTTCCCCAAGGTACAA CGGGACGGTTCACCAACGGTCGCACCTACGTTGATGCACTAGCTCAAATTCTTGGATTTAGGAACTATATTCCACCATAT TCCAGGATTCGTGGCCAGGCTATACTTAGAGGTGCGAATTTCGCATCTGGTGCAGCTGGCATTAGAGATGAGACCGGTGA CAACTTGGGTGCACATACTTCTATGAACCAGCAGGTAGAGCTATACACGACCGCGGTTCAACAGATGCTTCGATACTTCA GAGGAGACACAAATGAGCTCCAGAGATACTTAAGCCGTTGTATCTTTTACTCGGGAATGGGAAGTAACGATTATCTCAAC AATTACTTCATGCCTGACTTTTACTCAACCAGTACCAATTACAATGACAAAACCTTTGCTGAGTCTCTGATAAAGAACTA CACACAACAGCTCACGCGATTGTACCAATTTGGAGCTCGGAAAGTGATTGTGACTGGAGTGGGACAGATCGGTTGCATAC CTTACCAGCTCGCCCGCTACAATAATCGTAACAATAGTACTGGCAGATGCAACGAAAAGATTAACAACGCAATTGTGGTG TTTAACACTCAAGTCAAGAAACTAGTGGACCGTTTAAACAAAGGTCAGTTGAAGGGAGCAAAGTTTGTTTACCTTGATTC CTACAAGAGTACCTATGATCTCGCCGTCAATGGAGCCGCTTACGGATTTGAGGTTGTGGACAAAGGTTGTTGTGGGGTGG GGAGGAACAATGGTCAGATAACATGTTTGCCGCTGCAGACCCCGTGCCCTGACCGAACCAAGTATCTCTTTTGGGACGCG TTTCATCCGACGGAGACTGCTAACATACTGCTGGCGAAATCTAATTTTTACTCCCGAGCTTACACTTACCCAATCAACAT CCAAGAACTAGCCAATCTTTGAAGAAATAATATCTTGATCTCCCATATGAATGCATATATGTCATGTAATATGGCTATTT ATAAAATGTTTCTATTTCCTATGTGTTAACTTATGTTTCTT .......................N...N.................................................... ...................................................N............................ ................................N............................................... .............................N.................................................. ...............................................N................................ ..i..................................................N.......................... .................................N.............................................. ...................................................................N............ ......................N.........................................N............... ............N...........................................i....................... .........N.................................N.................................... ................................................................................ ..............................................N................................. ................................................................................ ..............N..............N.................................................. .........N............i......................................................... ................................................................................ ..............................................N...N.......N....N......N......... .....N..............N...........N........ Pos Score Pred ------------------------ 24 0.450 - 28 0.366 - 132 0.136 - 193 0.193 - 270 0.331 - 368 0.243 - 403 0.677 Yes 454 0.426 - 514 0.247 - 628 0.363 - 663 0.248 - 705 0.182 - 733 0.384 - 777 0.676 Yes 810 0.235 - 844 0.375 - 1007 0.346 - 1135 0.212 - 1150 0.339 - 1210 0.248 - 1223 0.661 Yes 1407 0.057 - 1411 0.062 - 1419 0.105 - 1424 0.105 - 1431 0.116 - 1446 0.035 - 1461 0.040 - 1473 0.051 -

Explain the output -- Processing locus 518: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTGTAAAAAAAAAAAATCGATTTTACAAACAAAAAAATATACTTTATGAATGTGGTATTCATTTTAAATTTTTCGCCA AAATATTATTTATTTTGATAGCCAAAACGGCAAAACCCAAAAGGCCCAAAACGAATCTTAAATATGGTCCGGGTCATGGA TAATACAAAACGGGTCAGGCCTAATCATCTCTTTAAGGCCCAATATGGTTTAGTAAGGAAAGGACTCTCAATTTGGCGGG AGAGAGAAAGAGAAGCTTCTCTAATCTCTTCACCGTCTAAAGAATATTTCCGACGCCGGAGACGAAAACAAAAAATGGCA AACAAACTCATCGGAGAAATGGGTCTTCACACCAAAATCTCAAACATCTTCGCTGCAAGAAACATCATCACCGCCAAGGA TGCATTATCAATGACGGAATTTGAACTAATGGAGCTGTTAGATGTTGGAATGAAAGAGATAAGATCAGCAATTTCATTCA TCAGTGAAGCTACTTCTCCACCATGTCAATCTGCTCGATCTTTACTGGAGAAGAAGGTCGAAAACGAACATTTATCAGGT CATCTTCCTACACATTTGAAGGGGTTAGATGATACCTTGTGTGGTGGGATACCTTTTGGTGTTCTTACTGAGTTAGTTGG TCCTCCTGGTATTGGTAAATCACAGTTTTGCATGAAACTTGCGTTATCAGCTTCGTTTCCAGTAGCTTATGGAGGATTAG ATGGTCGTGTGATATACATAGATGTGGAATCCAAGTTTAGTTCAAGAAGGGTGATAGAGATGGGACTGGAAAGCTTTCCG GAAGTGTTTCATCTTAAAGGAATGGCACAAGAGATGGCTGGAAGAATCCTTGTTTTGCGTCCAACATCTTTAGCTAACTT TACTGAAAGTATACAAGAACTCAAGAATTCAATTCTTCAAAACCAAGTAAAGCTTCTAGTGATTGATAGTATGACAGCTC TTCTTTCAGGCGAAAACAAACCAGGAGCTCAGAGACAACCTCAGTTGGGTTGGCATATCTCTTTCTTAAAATCGCTTGCT GAATTTTCACGGATTCCTATAGTGGTGACTAATCAAGTTAGATCTCAAAACCGCGATGAAACTAGTCAGTATTCTTTCCA AGCTAAAGTTAAAGATGAATTCAAAGACAACACAAAGACATATGATTCTCACCTTGTTGCTGCATTGGGGATTAACTGGG CTCATGCTGTAACCATCCGACTGGTCCTTGAAGCCAAGTCAGGTCAGAGAATCATTAAGGTGGCAAAATCTCCTATGTCG CCTCCTTTAGCCTTCCCGTTCCATATAACATCAGCTGGGATTTCATTGCTGAGCGACAACGGGACTGAACTGAAAGGTCC AGGAATCAACACCATTCATGCTCGAGGGCACAGCGACATGATAAATTTTCACGGGGACTGCTCGTAGCTAAGCAAAGGTG GCCTGTTACGATTATCAACTACATCAACTTCCAAATGCTGCAAATAATCACTATAGTAAGTAATTAGTTCTCAATCCAAC CTTATT ...............................................N...i............................ ...............................................................i...........i.... ............................................i................................... ..........................................................................i..... ..................i............................................................N ..........i.................i............N.......i.............................. ......................N......................................................... ............................N................................................... ...............................N....................................N........... N....................N.....................................N.................... .....................N...........N.............................................. ......................................................................N......... ................................................................................ .......................................................N........................ ..............N..........................N...................................... ...N......................................................................N..... ................................................................................ .................i...................N.......................................... ..................................N............................................. ...... Pos Score Pred ------------------------ 48 0.178 - 52 0.644 Yes 144 0.690 Yes 156 0.595 Yes 205 0.762 Yes 315 0.710 Yes 339 0.540 Yes 400 0.197 - 411 0.540 Yes 429 0.523 Yes 442 0.408 - 450 0.663 Yes 503 0.291 - 589 0.319 - 672 0.408 - 709 0.123 - 721 0.298 - 742 0.287 - 780 0.168 - 822 0.301 - 834 0.360 - 951 0.384 - 1096 0.190 - 1135 0.412 - 1162 0.223 - 1204 0.106 - 1275 0.475 - 1378 0.514 Yes 1398 0.154 - 1475 0.085 -

Explain the output -- Processing locus 519: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCATAATTCTCCGGCGAGTCAGATCGACGGTGAGTTCTTCAGACCAAGCTCGATTACATGATTGTGTGTCTGATTTTGGG ATCAGTTCTGAAACTATTTGATACTTTAGGATTTTGTTTCTTGCGATGAATTGCGATAGAATCTGCGAATTGCCAGATTC TTTACTAACTCAAGTACTCTCATACCTTCCGACCATAGATTCCGTTAAAACAAGCGTTTTATCGAAACGATGGGAATTTC TCTGGCTTAGGGTTCCTGTTCTCGACTTAAAAGTCAGCGATTTCCCTGACGAAAACTACGCAAGCTTTATCGACAATTTT CTCGAGTTTAATCGAAAATCACGAATGCGAAAATTCAAGTTGAAGTATGATGAATACACTTACGACGATGATCGGTTAGC TGGGTGGGTTGTAACAACGGTTGATCGCGGAATTCAACATCTAGATGCTAAAGGTTTTGAGACTAATATGTGTGTAAGAG AGTTCATGCCTCAGAATATTTATAAGTGCAATACATTGGTGTCTCTAATGCTTGTAACCGTAGGAATCGAGAATCCAGAG TTCGTTGTTTCACTACCGTCTCTTAAGATAATGCATTTAGAAGATGTTTGGTATTATGATGATCCTTTGATCATGGAGAA GATTATCTCAGGTTGTCCTGTTCTTGAAGATTTTGTATTGATTAGGCCTATTGATTTTTGTAATCTAGATGTTCTGCAGT TTCTTCGTGTGAGGTCTCTGAGTCTTAGAAGCTTTCGTCTTACGTTTGAGTATAGTGTTTCTTGTACGTATTTTTCGGTT GAGATTGATGCTCCTAGACTTGAGTATTTGAATTTTAATGATGACCAATCTGATACGATTGTAGTCAAGAACATGACTTC TTTGTCGATGATTGATATTGATAGTGAATTTAATGTTAAGTTTGGCGGCAGTCGTTTGGAACCGGGGGATTTACGCAAGA GAGATATCATCCGTGATTTTTTAACCGCGATATCTTGTGTAAGACATATGATCATCTCTCGGCGTACTTTAGAGGTCCTA GATCGATATTCGAAGTTGGTACCTATTCCGAAATTCGATAACTTGTACCGTTTACAAGCTGCGGTTTCTCGATCCATGTT GCAGTTGTTGCTTGTCTTTCTTGAGAGCTGCCCAAATCTTGAAAACCTCATCTTGGACTTTACTGTTTCAACGGAGCCTG AGCAAGATGGACTTACCTATGTCCCTCAGTGTTTGTTATCGAGTTTGGAGTGTGTTGAGATTAGAGAATTGATTATGGGA GAAGAAACCGGGGAGAAATTAGTGAGGTATTTTCTCAAGAATTCAGTAGTTCTCAAGAAACTCATTTTGCGTTTAGAAGA TTCTTCGATAGCAAACCAAGATTCAGATATCTTCAAGGAGCTTAGTACTTTCACAAAGCGTTCTCGCAGCTGTGAGGTTA TCATCATTCATTGACAATGTTTATAGAAGATCATTGTAGAAATCTTTTGTTATGAAAAATTTGCAATTAGGCTTAGCCTT TTCCAGACATTGGTGTGGATAATATTAACTGTA .........................................................N...................... .............................................N.................................. .....................................................................N.......... ................................................................................ ........................i.....................N..N.................N............ ............................................N......................N............ .....i.........................................N................................ ..............................N............N...........N..N.............N....... ....................................................................N........... ................................................................................ .......N.............................N..N...............................i....... .......N........................N............................................... ...............................................N................................ ...........................................................................N.... ................................................................................ ......N...........N.......................................................i..... ................................................................................ ................................................................................ ................N..................................N............................ ................................. Pos Score Pred ------------------------ 58 0.175 - 126 0.423 - 230 0.413 - 345 0.602 Yes 367 0.282 - 370 0.329 - 388 0.378 - 445 0.141 - 468 0.388 - 486 0.591 Yes 528 0.345 - 591 0.112 - 604 0.237 - 616 0.077 - 619 0.062 - 633 0.277 - 709 0.079 - 808 0.059 - 838 0.091 - 841 0.170 - 873 0.604 Yes 888 0.431 - 913 0.234 - 1008 0.189 - 1116 0.425 - 1207 0.178 - 1219 0.247 - 1275 0.617 Yes 1457 0.400 - 1492 0.092 -

Explain the output -- Processing locus 520: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTATGATATATGAACAGACTCCGCTCTGTTTAGCTTTTATAAAATTGTTACCTATTTATACAAAATCAATGTAGCTATC GTCTCTAACATGGCTGTGTAAAAAGTGACCTTTTTCATTGTATCAGTGCAGATTTTGTATCAAGACATGTCAGCTACTTT GAAGAATCCGGTGATTAAGTTGAAACTACTCATAGATGAAGAGAAAAACAAAGTTGTATTTGTTGAGGCGGGTAAAGATT TTGTCGATTTACTCTTTAGTTTTTTTACTTTGCCAATGGGAACGATTGTTAGATTGCTGGAGATGTATAAGAAGTCACAG AAAATAGCAATAGGCTGTTTCAGCAATATATATGCAAGTGTTGTGAGTATGGGCATTGAGCATTTCTTGACTGAAGCTTG TAAGCAAATGCTTTTGTATCCGGGCAGTTTGAATCATGAGAAATGTCGAAACCTTAAGCTGAGGGTAGATGATTCCGAGG CTACCAAGTATTTCGTGTGTCCTAAGTTTGTGGAACGTGAGCAATGTACTGAAAGTTACAGCAATTTTAATACCTCGAGA TGTAGCTGTGGTGTTTTGATGAATGAAGTGACTCAGCTTGATGGAAGAGGAGGCCTAGCCAGTGCAGGAAATGGTGTTGA AGGTGGAGTATTTGTTAGGAGTGATCATACTTCATTCATGATCACTGATGATTTGAAAGTGGAAATTACCTCTGTTAGGT TGACACTTAATGTTCTTAAAGATCTTGGCTACGTAGATTGTGAAAAGTTGGATGAGAAGATTCATGACGTTAATCTCGAA GAGGTACCGACTTTGCTGGAGTGTTTGTCTACATCGGATAATCCATTGACCGATACATTTCTGAAAAAGAAAAGTTCATA TAGTACAAAGAGGATTCAGAAATTGCCGAGTCCTGGCCTCTGTGAAGATAGAGAGGAATCAACACCTGACCAAACGATTA CACTAAATGTTGTAAAGAAGGAAGGAAACATTCTGTTTGTTGAATGTGGTGATGACTTTGTTGATCTTCTCTTCACTTTT CTTGCTATACCTCTAGAATCTGCATGGGGAATAGCTGGCGATGGAATCATCATTGGATGCATTGGAAACTTGAGCAAAAG CTTTAAGGACTTAAGTGTTGATCCCGGACGAGAAGCTAAATGTGTTCTTCCTCATTACTACAAGTGTCAGAAGCAGCTAC TTGATGCTGTCACCACCCATAAGCCACCGACTTTCTACTTGTATGTGTCCTCCATGGCTTCGAATCATATTAGAGAGTAC CGTTTGTCAGAAGACTCCAGTAAGCCACTTGTTTATTTTTGGGATGAACTCATGCCCATGATTTCGATAGATCCAAAATC TAAAGGTAGTACTAACACTACTGATGAGTCAACAATACCCAGTGGTGGCTTTATGAAGAGAGGGACAAAGTTTATGATAA CAGATGATTTGATCATCACACCTTTGAATTCAACCTCAACCATTGGATTGGTAAAAGAAAAACAGATCCGATTAGATGAT GTCGAGGTTCAAGAGATCATCATTAGAAAAGAAGAGGCCATTAGGTTATTACAAGCTTCATTGGTGACATGTTCTGCCTT AAGCACTGGTCTTCTAGCTATAGAATCAGCTTCAACAAGTGTTCCTCAAAGCGCAAATTTGAAGAAGCCAAAGATTGAGA CTTGAGGAGCAATGTTTAACCAGAAACTTCGAGTTCTTGCTAAATTTGATTGCCAGAGAATCTTTTGATAGCTTATGAAT GCTCAGTTTAATTTATTAGTTTCT ...N......N..........................................................N.......... .........i........................................................i............. ...................................N............................................ ...................................N..........................N................. ...............................N................i............................... .......N...........................N......i.........................N........... ...........................................N...................................N ..................i...N.................N.............................N......... .....................................i.........N................................ .........N.........................................N...........N................ ................................................................................ ................................................................................ ......N....................................N.......N............................ .......................N................N...............N....................... .......................................N........................................ ...N......................................N..........i.......................... ...........................................N.......i.....N...................... .......................N............................N....................N...... ...N.......................................................................N..N. ....................................................................N........... ................................................................................ ...........N..............................................................N...N. ........................ Pos Score Pred ------------------------ 4 0.206 - 11 0.219 - 70 0.108 - 90 0.651 Yes 147 0.692 Yes 196 0.240 - 276 0.297 - 303 0.287 - 352 0.200 - 369 0.522 Yes 408 0.309 - 436 0.299 - 443 0.683 Yes 469 0.336 - 524 0.349 - 560 0.141 - 579 0.582 Yes 583 0.203 - 601 0.112 - 631 0.054 - 678 0.684 Yes 688 0.169 - 730 0.119 - 772 0.094 - 784 0.060 - 967 0.077 - 1004 0.391 - 1012 0.345 - 1064 0.254 - 1081 0.366 - 1097 0.151 - 1160 0.382 - 1204 0.215 - 1243 0.190 - 1254 0.754 Yes 1324 0.190 - 1332 0.515 Yes 1338 0.337 - 1384 0.379 - 1413 0.259 - 1434 0.430 - 1444 0.112 - 1516 0.234 - 1519 0.260 - 1589 0.471 - 1692 0.131 - 1755 0.056 - 1759 0.038 -

Explain the output -- Processing locus 521: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCGTATGAGCTGGACACTGGAAAAAAAGATAAAATATATGAGGAGGAAAATGGAATTAATGTTTAGTCGAAAGTTGAA ACTGAAATAGACATGACATGATCACATACTCACATGCTAACAGAGAAAAAGAAACAGAAACAGAAAGAGAATAAGACGTC ACTATCTCTCGAGGGAAGAATTCCTCCACAAATAGAAAGAGTTACTGAATATTCTTTGAACATGCGCTACATGAAAGACA ACAACAACAACAAAAAACTAGTAGATATATATTATTACTCGAAGTCGATGAAAAAACCGTCGTTGTTTCAGATCATTATT ATAGTGCTAAGCATACCAACGACTACGGGGAGGACGGTGGGAAATCTTCGGGTGCGGGAAGGATCACCTTTCAAGATCGC AATATTTGCAGACCTCCACTTCGGTGAAGACACGTGGACTGATTGGGGTCCTGGTCAGGATGTGAACTCCGTTAACGTTA TGTCCGCCGTTCTCGACGCTGAAACTCCAGATTTTGTGGTCTATTTGGGAGATGTTGTGACGGCTAACAACATAGCAATC CAAAACGCAAGCTTGTTTTGGGACAAAGCAATCTCTCCTACAAGAGACAGAGGCATTCCATGGGCTACTCTCTTTGGTAA CCATGACGATGCTTCTTTTGTTTGGCCTTTGGATTGGTTGTCTTCCTCCGGTATTCCTCCCCTTCGTTGCCCTGCAGCAT CAGATGATGATGGATGCACGTTCAGAGGAACTACGCGAGTGGAACTGATTCAGGAAGAAATAAAGTCATCCAATGCACTC TCATACTCCATGATTAGTCCTAAGGAGCTATGGCCAAGCGTATCTAACTATGTCCTTCTTGTGGAATCATCGGATCATTC GAAACCACCCGTGGCATTGCTATATTTTCTTGATTCGGGCGGTGGTTCATACCCGGAGGTTATCTCAAATGCTCAAGTGG AATGGTTTAAAACCAAGTCTAATACTCTTAATCCCTATTTAAGGATCCCGGAGTTGATATTTTGGCACATACCTAGTAAA GCATACAAGAAAGTAGCTCCAAGGTTATGGATAACAAAACCTTGCGTTGGATCAATCAACAAAGAGAAAGTTGTTGCTCA AGAAGCTGAAAATGGGATGATGAGAGTTCTTGAGAACAGATCCTCCGTTAAGGCAGTGTTTGTAGGACACAACCATGGAC TAGACTGGTGCTGTCCGTACAAAGATAAACTCTGGCTTTGCTTTGCGAGACACACAGGTTATGGTGGATATGGAAATTGG CCAAGGGGATCAAGGATTTTGGAGATTTCTGAAATGCCCTTTCGTATTAAGACCTGGATTAGGATGGAAGATGGATCTGT CCACAGTGAAGTTAACCTAACATATGATTAGTCTATTTATCACATATGTCAAACTTTGTAGAAGTAATAGTGCCTATAGT TGTTGGTAGGTTTGGTCACTGTAAACTTGTTAAGTGACAAGAAAAACTGC ......N................................N...........N........N................... ............i....i...............N.............................................. .............................................................N........N......... ...............................................N................................ ................................................................................ ...........................................................N...................i ...................................................N............................ ...........................................................N.................... ..i.....N....................................................................... ...N..N..N...N..........................................................N....... .........N...................N...................i.............................. ....................................................................N........... ..........................N..................................................... ...........N....N..i......................................................i..... ............................................................N........N.......... .................................N.............................N......N......... .......................N.....................N.................................. .................................................. Pos Score Pred ------------------------ 7 0.131 - 40 0.323 - 52 0.310 - 61 0.274 - 93 0.536 Yes 98 0.639 Yes 114 0.202 - 222 0.294 - 231 0.114 - 288 0.399 - 460 0.119 - 480 0.730 Yes 532 0.218 - 620 0.141 - 643 0.520 Yes 649 0.302 - 724 0.223 - 727 0.368 - 730 0.207 - 734 0.488 - 793 0.216 - 810 0.188 - 830 0.462 - 850 0.576 Yes 949 0.213 - 962 0.585 Yes 1067 0.317 - 1132 0.492 - 1137 0.355 - 1140 0.504 Yes 1195 0.779 Yes 1261 0.363 - 1270 0.448 - 1314 0.352 - 1344 0.108 - 1351 0.343 - 1384 0.181 - 1406 0.137 -

Explain the output -- Processing locus 522: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATGTTTTTATGATTTTATTTTGTAAATCCAATTTATATACTATACAACATATAATATATCTTTTTTTGTTTGTTTAAGC CTAAATTGACAATAACTGTGAATGTTGTTATTCAGGTATGGAAAAGTGTTCAAGTCAAATATATGTGGTGGAAAAGCAGT AGTCTCATGTGACCAAGAACTCAACATGTTCATACTTCAAAACGAAGGGAAGTTGTTTACATCGGATTATCCAAAAGCGA TGCATGACATTCTCGGCAAATATTCCCTTCTATTAGCCACCGGAGAAATTCACAGGAAACTAAAAAATGTTATTATTAGC TTCATCAATCTCACAAAGTCGAAACCTGACTTTCTTCACTGCGCAGAGAACCTCTCTATCTCGATACTAAAGTCATGGAA AAATTGCCGAGAAGTCGAATTCCATAAAGAAGTTAAAATATTTACTCTCAGTGTTATGGTAAACCAACTCTTGAGCATCA AGCCAGAAGACCCAGCAAGACTTTATGTATTGCAAGATTTTTTATCTTATATGAAAGGGTTTATCTCCTTACCAATACCG CTTCCAGGAACGGGTTATACAAACGCAATTAAGGCTAGGAAGAGATTGTCGGCGAGGGTTATGGGGATGATAAAAGAAAG AGAGCGCGAAGAAGAAGACATGAATAATGCAATAAGAGAAGAAGATTTTCTGGATTCGATAATTTCGAATGAAGATCTAA ATTATGAAGAGAAAGTTAGCATTGTGTTGGACATTTTGCTTGGAGGCTTTGAGACAAGTGCTACTACTCTTTCCTTAGTC GTCTATTTTCTAGCAAAATCTCCAAATCTTCTTCACAAACTCAAGGAAGAACATGCAGCCATTAGAGCCAAGAAAGGGGA TGGGGAACTTTTGAATTGGGAAGATTATCAGAAGATGGAATTCACTCAATGTGTGATTTCTGAGGCACTACGATGTGAAT ATGTGATTCCAAAGGGGTGGAAGGTGTTTCCAATCTTCACAGCAGTACATCTTGATCCCTCTCTTCATGAAAATCCTTTT GAATTTAATCCCATGAGATGGACCGATAAGGCTAAGATGAACAAGAAAACGACGGCGTTTGGAGGAGGAGTAAGGGTATG TCCTGGTGGTGAACTTGGCAAGCTCCAAATTGCTTTCTTCCTTCATCATCTTGTCCTCTCCTATAGGTGGAAAATAAAGT CAGATGAAATGCCAATCGCGCACCCTTACGTGGAGTTTAAGAGAGGCATGCTTTTGGAGATAGAGCCAACAAAATTCCTT GAAGATTAGCTGCTTTAATAAGGAATCCATCTTAGATGAAAATTTAATTAATAACTATTAAAAATGTAATGTCTAATAAT ATGCTCCATTCCCAAATATAAAGTACTC .....................i...............N........................i................. ......N..................i.....................................................N ...N..............................................................i............. ..........................................................................i..... .......................................................N........................ ........................N.........................i............................. ............................................................i.....N............. ...................N......N.........................................N........... ...N............................................................................ ....................................................N..........................N ..................................N.............N.......................N....... N.................................................................N............. ............i....i..................i........................................N.. ................................................................................ ...N....i......................................N................................ ...................................N...........................N....N........... N........................... Pos Score Pred ------------------------ 2 0.135 - 10 0.199 - 102 0.654 Yes 118 0.446 - 143 0.549 Yes 167 0.176 - 186 0.558 Yes 240 0.430 - 244 0.316 - 307 0.711 Yes 395 0.660 Yes 456 0.266 - 505 0.368 - 531 0.532 Yes 621 0.537 Yes 627 0.224 - 660 0.467 - 667 0.232 - 709 0.150 - 724 0.160 - 853 0.352 - 880 0.362 - 915 0.466 - 929 0.432 - 953 0.162 - 961 0.157 - 1027 0.110 - 1053 0.566 Yes 1058 0.622 Yes 1077 0.838 Yes 1118 0.451 - 1204 0.471 - 1209 0.767 Yes 1248 0.177 - 1316 0.056 - 1344 0.104 - 1349 0.068 - 1361 0.054 -

Explain the output -- Processing locus 523: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATATAAGTATAAACCAAACAATAAATGAGCATAAAATTTCTAACGTTCCAAACAAAAATAAACCCCAAAACTTTCAAA AGGTTACAAACAACCCCCTAAGTAAAGAAAATGACGATCAATTTAGTCCCTCTTCTCCTCTCCCTAATCACCATCTTCAC CATCTTCAATCCATCCGCCCTTGCAGACCAGAGTCAAATCCGCAGCACAACAAGCCAATGGCTCCATTTCTCGGAGGACC TCAGCCAATCAACTATTGAAGTCTCCATCCCCACCATAATCGCCGCCGTTCTCTCGTTCTTCGCCGCCTCTATCTCCAGC GCCGGAGGAATCGGCGGCGGTGGACTCTTCCTCTCGATAATGACAATCATCGCCGGTCTCGAAATGAAAACAGCTTCAAG CTTCTCAGCGTTCATGGTAACCGGCGTCTCTTTCGCTAACGTGGGATGCAATCTTTTCCTGAGAAACCCTAAATCAAGAG ACAAAACCCTAATCGACTTCGATTTAGCTCTTACTATTCAGCCATGTCTACTTCTCGGAGTAAGTATTGGTGTGATCTGT AACCGTATGTTCCCAAATTGGCTTGTGTTGTTTCTCTTCGCTGTGTTCCTCGCCTGGTCAACGATGAAGACTTGTAAAAA GGGAGTTTCTTACTGGAATTTGGAGTCGGAAAGAGCGAAGATTAAGAGTCCAAGAGACGTTGATGGCATCGAGGTAGCGA GATCGCCGCTTCTGAGTGAAGAAAGAGAAGATGTGAGACAGAGAGGGATGATTAGGTTTCCATGGATGAAGCTTGGAGTT TTAGTGATCATTTGGTTATTGTTCTTCTCTATTAATCTTTTCCGCGGAAACAAATACGGTCAGGGAATCATATCGATCAA GCCATGTGGAGCTCTCTACTGGTTCCTCTCCTCGCTTCAAATACCTCTCACTATCTTCTTTACTCTCTGCATTTACTTCA GTGACAATGTTCAAAGCAACCACACATCGCATAGTAACCAAAACTCTGAACAGGAGACTGGAGTTGGAGGAAGACAAAAC AAGTTGATGCTCCCTGTAATGGCTCTCTTGGCTGGAGTTTTGGGTGGCCTCTTTGGTATTGGTGGTGGAATGCTCATTAG TCCTCTTCTTCTCCAAATCGGTATTGCTCCAGAGGTAACTGCAGCTACATGTTCGTTCATGGTTTTGTTTTCTTCATCAA TGTCTGCAATCCAGTACCTACTACTAGGCATGGAACATGCTGGAACCGCTGCGATATTTGCTCTGGTCTGTTTCGTGGCA TCACTGGTTGGACTAATGGTGGTTAAGAAGGTTATAGCCAAGTATGGGAGGGCTTCGATCATTGTGTTTGCAGTTGGTAT CGTCATGGCACTTAGCACTGTACTGATGACAACCCATGGAGCTTTTAATGTTTGGAATGATTTTGTCTCTGGTCGCTACA TGGGTTTCAAATTACCCTGTTAAGAACCAAAATTAAATAAATCCAGTTCTTGTTTATAACAGAACTTGCTTTTATAAAAA TTTACACTTACACTTGATATTTCACACAAACCAAATACCAAA ..........................N..................................................... ..............................i................................................. .........................................................i...................... ................................................................................ .......................................N.......................i................ .............N...............................N.................................. ...........................................i.................................... ......N........................................................i................ ..............................................................N................. ..............................N................N.............i...N.............. ................................................................................ ...N............................................................................ ......N......................................................................... ......N...........i..................................................N.......... ................................................N.........N....................i .............................N......N........................................... ...............i...........................N.................................... ....N....................N.........N...........N........N......................N ................................................................................ .......................................... Pos Score Pred ------------------------ 27 0.454 - 111 0.641 Yes 218 0.720 Yes 360 0.436 - 384 0.610 Yes 414 0.327 - 446 0.429 - 524 0.870 Yes 567 0.410 - 624 0.811 Yes 703 0.231 - 751 0.387 - 768 0.342 - 782 0.567 Yes 786 0.177 - 884 0.449 - 967 0.353 - 1047 0.495 - 1059 0.619 Yes 1110 0.307 - 1169 0.467 - 1179 0.441 - 1200 0.648 Yes 1230 0.369 - 1237 0.456 - 1296 0.713 Yes 1324 0.223 - 1365 0.415 - 1386 0.417 - 1396 0.155 - 1408 0.417 - 1417 0.450 - 1440 0.156 -

Explain the output -- Processing locus 524: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGAGAACGTGAGAAACGTCCGTTCGAGAACAAGACAAAACAAACAAGAAACGAAAGCACATGTGACATGACATGATTCA TGAACATGATTATTTGATTAGTCAGCTTTCTTTGCCAAATCTGAATTCGACGTTTTACTTACAGACTTCTCTTGTCCAAG TTCCTCATTCGTGCTCCAAAGTTACTTCATCAAATTCGTATACTACTCTCTGTTTTTTAATGCTCTTTGCTCCAACATAC GGACTCTTTTAGGTATGGTTTTGATTTTGGGTTCTTTATTTATTTTCTTCGACAAAAAGATATTAAACTCTGCGGACATT AATCTCAACTCGTCGAACATTTTACTAGATTATATAATGGCGGGAGAGGTCCAAGATCCGTTTAACCTAAGCCTTTTGGA GAATTCTATACACTCGGGATCAGTATCATTTGGAAGATTCGAGAAGGAATCTTTATCATGGGAGAGGAGATCATCCTTCT CTCGCAATATATATCTTGAAGAGGCAGAGAGATTCTCTAAACCAGGCTCTGTTACTCAGATGAGAGCTCATTTTGAGGCG CATTTTAAGAAGAAAGGGATCCGTTTTCCGAGTTCTGTTGAATCTCAAACATGGGGAGAAGTTGTTCATCATCAAACTTG TACTGAGAAAGATGAAAATTTGTGGGAGAACACAAGTCAATTCGGTGATTCTTGTGTGAGTTTTGACGAGACGATTCTTG TGAATTCTGATGATGATGTTCATACTTCTATTTCTGTTGCTTTGGAGAGAACTGATATTGGTCGCATTGAAGATGAAAAA GAAACAAAATCTTCTTCCTCCGCAACTAGATCGAAGCCTTTGAAAAATGTTCAAAAAGCTCGTCCTTATTCTGCGACGAA TAAAGATTCTACCAAGAAGCATGTTGTCATACCAAAGGGATATTCTCCAAGTTCTAATGCTAAAACAAGTGTTGACACTA AGAGACAAAAGGAATTGAAGCCTAAGAGGATTGTTGAAACCGTTGCATGTCAAGCTCCAGCTACATCAAAGAAAACTGAG ATCCTAACTCCTTTGTTGGCTACAAGAGAAAAGAAAACAAGTACTAATGGTTTTAGTTTACGAAGTAACGAACGGGCCGA GAAGAAGAAAGAGGAGAAGGTGGAAGCTGTGGTACGTAAAGCTCTTAACTTAAAAGCAAGACCATTGCCTAAAAGCACAC AAGCTAGACCTCAACACACGTCAACAGGTCAGGCAAAGGCAAAGGCAAAGGCAAGAGATGATCACTCATCTACAGCTAGT TGTGATCGGTCATTGACAAATAGAATGGCCAAAAGTAAGGTCATTATTAACAAACAGAAGGCGGATATGCAGAGAAGTCT AAATGGAATTCGGCCAAAGACAAGCGACCAGACCGCGAGAAACAGCACGAACATAAGGAGTTTAGCGGTTAGGCGCTCTG CGGTTCAGGTTGCTTTGTGAGCAATTTCACTGTCTCTGCTTTTGTTCTTTAGTACTATTGGCCTCAAATGTGTCTTTGTG TTTGTGTTTCTAGGCTGTAATGAGTAGAATGTTACATCA ............................................................N......N....N......N .....N.......................................................................... ...........................................................N.................... ..............N................................................................. ....................................i........................................... .........................................................i...................... ...........................................................N.................... ..................................................N............................. ...........N.................................................................... .........N..N..N........................................................N....... ..............................................N................................. ....................i...................................N....................... ..............................................N................................. ..............................................N................................. ................................................................................ .........................................................N...................... ........................i.........................................i............. ..i............................................................................. ...................................................................N............ ...................N........N.......... Pos Score Pred ------------------------ 61 0.167 - 68 0.190 - 73 0.197 - 80 0.309 - 86 0.128 - 220 0.141 - 255 0.241 - 357 0.646 Yes 458 0.668 Yes 540 0.234 - 611 0.362 - 652 0.046 - 730 0.151 - 733 0.190 - 736 0.112 - 793 0.457 - 847 0.249 - 901 0.506 Yes 937 0.170 - 1007 0.373 - 1087 0.441 - 1258 0.276 - 1305 0.687 Yes 1347 0.662 Yes 1363 0.546 Yes 1508 0.100 - 1540 0.044 - 1549 0.074 -

Explain the output -- Processing locus 525: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGATTTATGGCGAACATTTTAGCTAAGTTTATATGCATGCATGAACCATTATGTTTCTATTTTAGCATGGTATTTAAT ACGTACAGACTGCTAATTTAAGAGGAAATTATATATTTATATGTACACAAAGAAACATATCATAGTCTTCCATATCTCCT CTTGACACTTTTCAATAATTTTCTATCATCGATCATGAGTTTCTCCACAACCACAAAGATGCAAATTTTGTTGTTGTTTC TCGTAATTCAGCTGTGCCATGGCTCAAATGACACTGTGAGTATCTCGCTGAAAAGGCATACTCTGAATGTCGGGGGTACT TCTTTTGGTGGTTTAAAAAACTTTGATGGAGTTGTCTTTTACGGTGAGATTTCTGTGGGTAGTCCGCCACAAAAATTCAA CGTTGTTTTTGACACCGGCAGTACTGATCTGTGGGTTCCTTCTAAGGAATGGCCTGAGGAAACCGATCATAAACACCCTA AGTTTGATAAGGATGCATCCAAGACATGCCGTTTGATGAAAGGGGGAGAAGTAAATATTGCATATGAGACAGGAAGTGTC GTCGGTATTCTTGCACAAGACAATGTTAACGTAGGCGGGGTTGTAATAAAAAGTCAAGATTTATTTTTGGCACGTAACCC AGATACTTACTTCAGAAGTGTTAAATTTGATGGAGTTATTGGGTTGGGCATCAAATCTTCACGTGCGCAAGGATCTGTCA CGGTTTGGGAAAACATGGTGAAACAAAAGTTGATAACTAAACCTATTTTCTCTTTATATTTGAGGCCACACAAGGGAGAT GGAGGAGAAGATCCAAATGGTGGCCAGATCATGTTTGGTGGGTTTGACCCGAAGCAGTTCAAGGGAGAACATGTTTATGT ACCGATGAAACTAAGCGACGACCGCTGGAAGATCAAAATGTCCAAAATTTACATTAATGGTAAACCAGCGATCAACTTTT GTGATGATGTAGAATGCACAGCAATGGTTGACTCTGGATCGACTGACATTTTTGGCCCGGATGAAGCTGTCGGTAAAATC TATAAAGAAATTGGAGCAACCAAAGTAATAATTAGATGCGAGCAGTTTCCTGCTTTGCCGGATATCTACTTCGAAATTGG AGGAAAACATCTACGCCTCACTAAACATGATTACGTTGAGGTGAAGACAAATCCCAAAAAAAGATGCAGACTTCGTATTG TGAAGTCTAAAAATCGTCGTAAAGACTGGGTCTTGGGTGAGGCTTTCATGACGAAGTTCCATACGGTGTTTGATTATGGA GATGTTAAAACCCCAAGAATCGGCTTTGCTGAAGCAATATAAGAAAATTAGTAAAAAGAGTACACTAAACAAGTTTTATC TAAATTTTATGACAATATATATGTGAACCAATATTGTTATTCTGTAAGCGTGACTTCTTAA ........N.........................i...N...N.........N...............N........... ........................................N....................................... ..................................i.......................N..................... ..................N........N......................................N............. .........................N...................................................... ................................................i............................... ............N............N.........i...........................N................ ......................N......................................................... .............................N.................................................. ..............N...............................................................i. ................i.............N.......................................N.....N... ....N................................i..................N....................... ...N..N......N.........i....................................N................... ...................................N............................................ ..........................N....................................i................ ...............................................i...........................N.... ........N...........N........................................ Pos Score Pred ------------------------ 9 0.358 - 35 0.526 Yes 39 0.283 - 43 0.257 - 53 0.190 - 69 0.171 - 121 0.376 - 195 0.531 Yes 219 0.411 - 259 0.375 - 268 0.205 - 307 0.268 - 346 0.208 - 449 0.546 Yes 493 0.259 - 506 0.453 - 516 0.565 Yes 544 0.194 - 583 0.189 - 670 0.108 - 735 0.418 - 799 0.675 Yes 817 0.607 Yes 831 0.443 - 871 0.207 - 877 0.233 - 885 0.498 - 918 0.850 Yes 937 0.392 - 964 0.259 - 967 0.207 - 974 0.371 - 984 0.818 Yes 1021 0.066 - 1076 0.459 - 1147 0.271 - 1184 0.543 Yes 1248 0.569 Yes 1276 0.340 - 1282 0.149 - 1369 0.034 - 1381 0.066 -

Explain the output -- Processing locus 526: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGCTCTTCTTCAACCTTGAAGACTCTGACCCCATTCTGCTTTACCCTCTCCAGGTCTCCCTTCCACCAATCCGGGGGCG ATGATGATGCTTCGGGATTGAAAAACCAATTGTTGCGCTTCCGTAATGATTCCGGTAAAGTTGCCTCCGTTTTGGAGAGG AACAAAATCCAAGGAGCTGCGTTTGTTGAGCTCCTTAGGCAGTTACGTCCATGGCCCGTTTTGTCCCAACTGGTATACAT ACATCTTTTTTGTCTTCGAATTTCACAACCACAAAAGAAAAAGCCTTTTTTCCGTCATGTTTGATGTTGTTGCTTTGCAG GTGTTCGACTGGAGAAGAAACAAGGCACTATGCGATGGGTTGCCTATGACTGCTGATGAATACGCTAAGGGCATTACCAT TTCTGGTAGACTGAAGAATGTCGATTTGGCCCTTTCCTTGTTTCACGAGTCTGCAAACAAGACCACCTCCGTCTACAATG CTTTGATGGGTGCTTACCTCTGCAACGGTTTGTCTCATCATTGCGAGCAGCTCTTCCTTGACTTCAATTCTCAACAAGAC GGACCCTCCTCTTCTACTCCATCTGTCTCTACTTACAACATCCTCATTTCTCTTTATGGCCGTTTGATTATGGTTGAGCG GATGGAATCAGTATTCCTACAGCTTCAACAACTCAATATCCTTCCCGATTCCAGCACCTACAACAACTTGATTGCTGGGT ACATCTATGCTTGGGATTGGGATAAGATGGAAGCAACTTTCCACTCCATGAAGAACGGCCTTGTTAAGCCTACGCTTGCC ACCTATTTGCTGATGCTCAGAGGGTATGCAAACTCGGGAAATCTGCTACGGATGGAGGACATGTATCAAGCCGTCAAGCG TCACGTTGACAGGAATGAGATTAAGTTAATCGAGTCCATGATTTGCGCATACTACAGGAGTTGTCACAAGGATAGGATCA GAAAAATCAAGACTTTATCCAAACTTATCCCCAAGAAAAGTTACAAGCCTTGGTTGTATTTGCTCTTGATGCAGGTCTAT GCTAAGGATGATAATCTACATGCCATGGAGAATTTCATAGACCAGGCTATTACAAAAGGACTCCAGATAGAGACAGATGG TATTATGAGATCAATAGTGGCTAGTTACTTCAGGTGCAATGCGGTTGATAAGCTTGCCAAATTCGTGCAGCGTGCAAACT CTGCTGGATGGAAAATGAGCAGGTCTATGTTCCATGGCTTAATGATCATGTATGGATCTCAAAAGCGTTTTAAAGAGATG GAAAACGTCCTCTCTGAAATGGAAAGCTTTAAAATAAGCCGGTCCAAGAAGACGCTTTGTATTCTGCTTAGAGTATACGC AGCAACGCATGGACAGGAGCATAAGGTTAATCAAGTCGCAGGAATGATGTTAAAGCATGGACATGATTTCCAACGACCTG AAGCGAGTAAAAGAGTTATGGGAAAATAGGATACTAGAGAGAGAGATATGAATAAATACAAGCAGCATATTTTGTTGGGA AGGATTGTATGCATCCAGGGATTCGATTTGAACCCTATAGCATCAAGG ................................................................................ N..N..N......................................i.................................. ..................................................N............................. ........................................................i......N................ .............................N....N..........i.........N........................ .................N...........................................................N.. .....i.......................................................................... .......................................................N.............N.......... ......N...................i....................N................................ ............N............N.........................i........N................... ..............N......................N.......................................... ....................................................................i.........N. .......N...........N....N...................................................N... ....N.................................N......................................... .......N......i...........N......i.......N.....N...N.........................i.. ..................i............................................................. ........N..................................i..i.........N.....N................. .................i.............................N................................ ........N....................................... Pos Score Pred ------------------------ 81 0.281 - 84 0.334 - 87 0.216 - 126 0.547 Yes 211 0.257 - 297 0.703 Yes 304 0.136 - 350 0.312 - 355 0.488 - 366 0.731 Yes 376 0.204 - 418 0.396 - 478 0.427 - 486 0.514 Yes 616 0.456 - 630 0.368 - 642 0.461 - 727 0.301 - 747 0.670 Yes 768 0.486 - 813 0.480 - 826 0.355 - 852 0.511 Yes 861 0.238 - 895 0.221 - 918 0.124 - 1029 0.511 Yes 1039 0.159 - 1048 0.174 - 1060 0.157 - 1065 0.463 - 1117 0.176 - 1125 0.401 - 1159 0.344 - 1208 0.359 - 1215 0.525 Yes 1227 0.297 - 1234 0.509 Yes 1242 0.250 - 1248 0.288 - 1252 0.214 - 1278 0.622 Yes 1299 0.670 Yes 1369 0.418 - 1404 0.585 Yes 1407 0.716 Yes 1417 0.429 - 1423 0.285 - 1458 0.521 Yes 1488 0.109 - 1529 0.044 -

Explain the output -- Processing locus 527: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGATTTCCTGGATGTTATTTAGAATTAATTAGTCCACCAAAGTGATAAGAATCCTAGTGTCTATAGAATCACCTTTCTT TGCTATATAAACATCCTTTGAGCAAACAAATTAAACAAACCACACAAAACAAAAAAAAAAAACAAATTGATGGGGATGAA GAAGGTGAAGCTATCTTTGATAGCTAATGAAAGATCAAGGAAAACATCCTTCATAAAGAGGAAAGACGGGATTTTTAAGA AACTCCACGAGTTGTCAACTCTGTGTGGTGTCCAAGCTTGTGCTCTCATCTACAGTCCATTCATACCGGTTCCAGAGTCA TGGCCGTCAAGGGAAGGTGCTAAAAAGGTGGCTTCAAGGTTTCTGGAGATGCCGCCGACAGCCCGAACCAAGAAGATGAT GGATCAAGAGACTTACCTTATGGAGAGGATTACCAAAGCAAAAGAGCAACTAAAGAACCTGGCTGCTGAGAACCGAGAGT TACAGGTTAGACGATTTATGTTTGATTGTGTTGAAGGCAAAATGTCCCAGTATCATTATGATGCAAAAGACCTTCAAGAT TTGCAATCTTGTATAAATCTATATCTCGATCAGCTTAACGGAAGGATCGAGTCCATTAAAGAAAATGGTGAGTCGTTGTT GTCTTCCGTCTCTCCTTTTCCTACTAGAATTGGTGTTGACGAAATTGGTGATGAGTCATTTTCCGACTCTCCTATTCATG CTACAACTGGGGTTGTAGATACTCTTAATGCTACCAATCCTCATGTTCTTACGGGCGATATGACTCCTTTTCTTGATGCG GACGCAACTGCGGTAACTGCTTCCAGTAGATTTTTTGATCATATTCCATATGAAAATATGAATATGAGTCAAAATCTGCA TGAACCGTTTCAACACCTTGTTCCTACTAACGTTTGTGATTTTTTTCAAAATCAGAATATGAATCAGGTTCAATACCAGG CTCCTAATAATCTGTTTAATCAGATTCAACGAGAATTCTACAACATAAATTTGAATCTGAATTTGAATCTGAATTCGAAT CAGTATCTGAATCAACAACAATCATTCATGAATCCGATGGTGGAACAACATATGAATCATGTTGGAGGGCGTGAAAGCAT TCCTTTCGTGGACGGAAACTGCTACAACTACCATCAACTACCATCCAATCAACTACCAGCCGTTGATCATGCTTCCACCA GTTACATGCCTTCCACCACCGGTGTCTATGATCCTTACATCAACAATAATCTCTAATCACAAAAGAGGGAGAAGAACTAG TCTAAGTGTTCAATTGTTTGTATTTGTGTTTTCTTCATTTCGAAAGACAAAAACTAATGTTGTTTATTAATAATG ............N................................................................... .....................................................................i.....N.... ..........................N..................................................... ...............................................................................i ................................................i..........................N..N. ...................i............................................................ .................N.......................i...............N..N................... ................................................................N............... ..................................................N..........................N.. ...........................N..............N................i...............N.... .................................................N.......i.....i...............N ..........................................................i..................... ................................................................................ ...........................N........N..............N......i..................... ....................................................................i........... .....N.....................N.................................................... ........................................................N...............N.. Pos Score Pred ------------------------ 13 0.183 - 150 0.725 Yes 156 0.293 - 187 0.269 - 320 0.758 Yes 369 0.715 Yes 396 0.391 - 399 0.211 - 420 0.536 Yes 498 0.231 - 522 0.693 Yes 538 0.098 - 541 0.149 - 625 0.361 - 691 0.127 - 718 0.089 - 748 0.088 - 763 0.075 - 780 0.778 Yes 796 0.142 - 850 0.121 - 858 0.826 Yes 864 0.673 Yes 880 0.070 - 939 0.522 Yes 1068 0.282 - 1077 0.321 - 1092 0.481 - 1099 0.512 Yes 1189 0.552 Yes 1206 0.498 - 1228 0.262 - 1337 0.053 - 1353 0.051 -

Explain the output -- Processing locus 528: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTATGCCCAAAATTTATTTTAGTTGAAGAAAAAGGGTTTACGATGCTAACCTAGCTAGTTCGTTATTAAATGTTCTAGA CTTGACTATTGGCCATTTTGAATAAAAAGTCCTCATATGTTAAATTTTCACATGTCGATCCAAGCTCTACATATATATAT TTACTCATCATTCCAAAAGCTTCCAAAGAAACAAAGAGTAACAATGAAACGAAACAGTATAAATATTCATCACGTTACTT CTTTTTCTTCGAGTCCGTTTTGGTGTGTTTTCTTTCTCGTGTTGTTATGTAAGACGTCCACCAACGCTTTGGTGAAGCAG CCGCCAAACGAAACGACTCCGGCGATAATTGTGTTCGGAGATTCAATCGTTGATGCCGGAAATAATGACGACATTATGAC AACGTTGGCTAGATGCAATTATCCTCCTTACGGCATCGATTTTGACGGTGGAATTCCTACCGGAAGATTTTGCAACGGCA AAGTTGCGACTGATTTTATAGCGGGAAAATTCGGAATTAAACCAAGTATACCGGCATATCGAAATCCAAATTTGAAACCA GAAGATCTCTTAACCGGTGTAACATTTGCGTCTGGTGGTGCTGGTTACGTTCCCTTCACAACCCAATTATCGGGAGGAAT AGCTTTATCGCAGCAACTGAAGTTATTCGAAGAGTATGTAGAGAAGATGAAGAAAATGGTTGGGGAAGAAAGGACAAAGC TCATAATTAAAAACAGTTTGTTCATGGTTATATGTGGCAGTAATGATATAACCAACACCTACTTTGGTCTTCCTTCTGTT CAACAACAATACGACGTCGCTTCCTTTACCACTCTTATGGCCGACAATGCTCGATCTTTTGCTCAAAAATTACATGAATA TGGAGCGAGGAGAATACAAGTATTCGGTGCACCACCAGTAGGATGTGTCCCATCACAGAGAACCTTAGCAGGAGGGCCAA CAAGAAATTGTGTTGTTAGGTTCAATGATGCAACAAAGCTCTACAATGTTAAGCTCGCTGCAAATTTGGGTTCTTTGTCA AGAACTTTAGGAGACAAGACAATAATCTACGTTGACATATATGATTCTCTCCTTGATATCATTCTTGACCCTCGACAATA TGGATTTAAGGTGGTTGATAAAGGATGTTGTGGAACTGGACTAATTGAAGTTGCTCTTTTATGCAACAACTTTGCAGCTG ATGTATGTCCAAATAGAGATGAATATGTGTTTTGGGATAGTTTCCATCCGACTGAGAAGACTTACAGAATTATGGCCACA AAATATTTTGAGAGATATGTATGATCTCGACAATCGTGTAAGTCATAGCTTGATTTATCGTGTCTTTTGATGTTTATTAT CTTATTAGACGTGACCTCGAAATGTTTTTCCAAAAGAATATGA ...N.......................................N..........................N......... ....................................N..............N............................ ...........................................N.................................... ..............................................N................................. ....................................................N...........N..........i.... ............N................................................................... ................................................................................ ................................................................................ ...................................N..........N........i........................ .......................N.......N..........N..................................... ....................................i.........N..........................i.....N ..........................................N..................................... ........................N..N.................N.................................. ........................................N......................................N ........................N...................................N................... N...N.............N.....N..............................................N........ ................N...N................................................N.......... .....................N.................N... Pos Score Pred ------------------------ 4 0.372 - 44 0.241 - 71 0.253 - 117 0.141 - 132 0.427 - 204 0.458 - 287 0.493 - 373 0.129 - 385 0.303 - 396 0.639 Yes 413 0.157 - 676 0.241 - 687 0.257 - 696 0.523 Yes 744 0.406 - 752 0.154 - 763 0.254 - 837 0.684 Yes 847 0.260 - 874 0.510 Yes 880 0.462 - 923 0.370 - 985 0.193 - 988 0.325 - 1006 0.094 - 1081 0.232 - 1120 0.328 - 1145 0.257 - 1181 0.208 - 1201 0.124 - 1205 0.305 - 1219 0.278 - 1225 0.443 - 1272 0.232 - 1297 0.258 - 1301 0.237 - 1350 0.045 - 1382 0.063 - 1400 0.045 -

Explain the output -- Processing locus 529: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGGGTGAATCTCGAATCATTGCTTAGCTTAATGAGCTCCAGATTGTTATGTTATTTTGGGAATCTTAATGTGATTAGGAT TCGTTAGATTGTTTCTGTTGATTTGAGGACCCTTACTTTGGCTCTGTGTTAATAATATATTTGACCCTGTTATTGATGGA TGACATGAATAAAGAGGTTTCTGCTCAAATCTTTGTGAGAGTAATTAGTTTGATAATGACGCTGATGATGATGACATTGC AGGCAAACGATCGTTCACCAGCTCAGTTTGTTAGGCTGGATAAACCGAGAGCCGTAGACGATCTCTATATAGGCAAGAGA GAAAAGATGCGTAGGTGGTTGTGCTGTGCTTGCCACGTTGAAGAACCTTACCATTCTTCTGAAAATGAACACCTCAGAAG CCCTAAGCACCACAATGATTTTGGGCATCACACTAGAAAACCACAGGCGGCTGTGAAGCCTGATGCTTTGAAGGAGCCTC CATCTATTGATGTGCCTGCATTGTCATTGGATGAGTTGAAAGAAAAGACCGATAACTTTGGATCAAAGTCATTGATTGGT GAAGGATCTTACGGCAGAGCCTATTATGCAACCTTGAAAGATGGAAAGGCTGTCGCGGTGAAGAAGCTTGACAATGCAGC GGAACCTGAATCAAATGTTGAGTTCTTGACTCAGGTCTCTAGGGTTTCCAAGCTGAAGCACGATAATTTTGTTGAGCTCT TCGGTTATTGTGTTGAAGGGAATTTCCGCATTCTTGCGTATGAGTTTGCTACTATGGGATCTTTACATGACATCTTACAC GGGAGGAAAGGAGTCCAAGGAGCACAACCAGGTCCTACGCTTGACTGGATCCAACGGGTCAGAATAGCAGTTGATGCAGC TAGAGGACTTGAGTATTTGCATGAGAAAGTTCAACCTGCAGTAATACACAGAGATATTCGATCTAGCAATGTGCTTCTCT TTGAAGATTTTAAAGCCAAGATCGCTGATTTTAATCTATCGAACCAATCTCCTGATATGGCTGCTCGTCTTCATTCTACC AGAGTTTTGGGAACCTTCGGTTACCACGCACCAGAGTATGCGATGACTGGTCAACTGACACAGAAGAGTGATGTTTATAG TTTTGGTGTGGTGCTTTTGGAGCTCTTGACTGGTAGGAAACCCGTCGATCATACGATGCCTCGTGGTCAACAAAGTCTTG TTACTTGGGCTACTCCAAGGCTAAGTGAAGACAAAGTGAAGCAATGTGTTGATCCAAAACTAAAGGGAGAATATCCTCCT AAAGCTGTTGCAAAGCTCGCTGCAGTAGCAGCATTGTGTGTGCAATATGAATCAGAGTTTAGGCCAAACATGAGCATTGT GGTAAAAGCTCTTCAACCATTGTTGAGGTCATCAACAGCAGCAGCTGTACCAGTCCAGGAAGCCTGATTCTTCTGTGCAA TAGCAACAATGGAAGATTGGGTTCGGTTTCAGTTGTTGCCATACCGTATAAATGTGTTCTTAAAGAGAGTCTTTTTGTCG AGGCTT ..............................N................N...................N............ ...........................................................................N...N ....N..................................................i........N..N..N......... ................................................................................ ......i.........................................................N............... ..............N...............................................N................. .........N....................N................................................. .........................N..............N................................N...... ..............N................................................................. .......................................N.............i............N............. .........................................................................N...... ....................N...............................................N........... ........................................................i....................... .....................................N....i...........................N......... .......................................................i........................ ...........................................i.................................... ..............................................N......................N.......... ................................................................................ ........N..........................................N............................ ...... Pos Score Pred ------------------------ 31 0.187 - 48 0.157 - 68 0.284 - 156 0.260 - 160 0.138 - 165 0.436 - 216 0.661 Yes 225 0.309 - 228 0.337 - 231 0.428 - 327 0.744 Yes 385 0.332 - 415 0.123 - 463 0.172 - 490 0.182 - 511 0.340 - 586 0.106 - 601 0.289 - 634 0.153 - 655 0.184 - 760 0.345 - 774 0.695 Yes 787 0.409 - 874 0.128 - 901 0.077 - 949 0.238 - 1017 0.870 Yes 1078 0.127 - 1083 0.507 Yes 1111 0.150 - 1176 0.634 Yes 1244 0.500 - 1327 0.085 - 1350 0.370 - 1449 0.120 - 1492 0.068 -

Explain the output -- Processing locus 530: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATAAAAATTTACTACATATGTTGATATTATACTTAGGTTAAAAAAAAGAAAGATATCCTATGTGGCAACCACCTACAAA CTTTATTATAAATATAAGTAGATGGCAAGGCGTGTGGTTGCCAAACACAAAGAAAAACATTCGACATTACAGAAAAGAAA AAACTATAAGAAAATCTTAATTAATAAAATAATATATTCAGGAGGGCGAGAGAGAGAGAGAGGGATGATGAAGGAAGAAC AATGGGCACCAGTGATAGTAATGTTAATATCAAGTGTTGCAATGGGATCAGTGAACGCTCTCGTTAAGAAAGCACTTGAT GTTGGTGTTAACCATATGATCTTTGGTGCTTATAGAATGGCTATTTCTGCTCTAATCTTGGTTCCGTTTTCTTATATTTG GGAGAGGAAAACAAGGCCACAACTAACGTTCATGCTTTTGTGCGAACATTTCATCAGCGGATTACTTGGGGCAAGTTTGA TGCAATTTTTCTTTTTACTTGGGCTTTCGTACACGTCAGCAACTGTTTCGATGGCTCTAGTTAGCATGTTACCTGCTATC ACCTTTGCGTTAGCCCTTATTTTCCGGATAGAGAATGCGCAAAACCTAAAGAGCAAAGCAGGCGTGCTCAAAGTAATGGG AACCCTAATTTGCATAATGGGAGCAATGTTGTTGACATTTTACAAAGGCCCAGAGTTATCAAACCCTCATTCTCATCCAC AAGCTCGTCACAACAACAACAATAACAATGGTCACGACCAAACAAAGAAATGGCTTCTAGGCTGTCTTTATTTAGTCATT GGGACAGTGTTGTTGTCTCTATGGATGTTGTTTCAAGGGAAATTAAGCTTTAAGTATCCTGGAAATAAATACTCAAGCAC TTGTCTCATGTCAGTCTTCGCATCATTTCAATGTGCTATTTTGAGTCTTTATAAAAGTAGAGACGTCAAAGATTGGATCA TTGAAGATAAATTCGTCATTTTGGTCACCCTTTACGCTGGAATAGTGGGACAAGCGATGTCGACAGTGGTAACGTCATGG TCCATAAAAATGACAGGAGCAGTTTTCGTCTCAACATTCAGCCCCGTCAGCCTCGTCGCCGCTACGTTATTCGATTTCCT CATCTTACACTCTCCTTTATACCTTGGCAGCATACTTGGATCAGTAGTGACTATAACTGGCCTCTACGTTTTTCTTTGGG GAAGGAAGAATGAGACAGATCAGTCTGTTTCGAAGACTTTGAACAGTTCCCAGTTTTCTCAGAATAAGGATAACGAAGAC CATACAATAGCAAATCATAAGGATACTAATTTGCCCGTTTGATATATATATATATCATATTTATAGAGGTAGCTAGATAT TTGCTATCATAATCGTGATAGTACTGTCATTGATCAGCTCTATATTATAAGACTTATGTTC ..................N.........................................i................... .....................N.......................................................... ................................................................i..i............ ...............N....................N........................................... ...............................N...............................................N ..................................................i..............N.............. ..................................N........................................N.... ................i........N...................................................... ...........................i.....................i.............................. ....................N...N....................................................... .......i......................N................................................. ........................................................i...................N... .........i...................................................................... ................................................................................ .........N...................................................................... ................................................................................ .......................................................N..... Pos Score Pred ------------------------ 19 0.161 - 61 0.586 Yes 102 0.412 - 225 0.501 Yes 228 0.563 Yes 242 0.416 - 261 0.307 - 282 0.337 - 319 0.140 - 336 0.145 - 357 0.305 - 432 0.143 - 480 0.138 - 531 0.739 Yes 546 0.396 - 595 0.249 - 636 0.383 - 657 0.655 Yes 666 0.476 - 748 0.562 Yes 770 0.637 Yes 821 0.188 - 825 0.087 - 888 0.509 Yes 911 0.375 - 1017 0.565 Yes 1037 0.393 - 1050 0.672 Yes 1210 0.319 - 1416 0.027 -

Explain the output -- Processing locus 531: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAACCCTAGCCCACGAAGGAGATGGCATTGGTTGCAAAAAATGATTGCTACCTTTGAAGTTTGAAACTTATTCTTCCT TGCACAAGTCGCTTTCGATGATACCTCACTCAAGTCACAGTCAACGAACAAAATATCTACAAAATGGGTCACACAGATCC ACCACTAACTTCAAAAATTCTTATCTTCACGACTACGCTTCTGTCTTTACTTCCTCTCTCAACGTACGTAGACAGAAAAA AACAAAACATCATGACAGAAAAATCTGCAGAACTCGTTCGTTTGAACGAACTCAAGGCTTTTGTATCGACAAAAGCAGGT GTGAAAGGACTTGTCGATACCAAAATAACCGAAGTTCCTCGAATCTTCCATATCCCTTCTTCTTCAACTTTATCTAACAA CAAACCTTCTGATATCTTTGGCTTAAACCTCACTGTCCCAATCATTGACCTCGGAGATGGTAACACATCTGCTGCAAGAA ACGTCCTCGTTTCCAAGATTAAAGAAGCAGCTGAGAATTGGGGATTTTTCCAAGTAATCAATCATGGTATTCCTTTAACT GTTCTTAAAGATATCAAACAAGGTGTTCGAAGATTTCATGAGGAAGATCCAGAGGTCAAGAAACAGTATTTTGCTACAGA TTTCAATACAAGATTTGCTTACAACACCAACTTCGATATTCATTATTCTTCTCCTATGAATTGGAAAGACTCTTTCACTT GCTACACTTGTCCTCAAGATCCTCTAAAGCCAGAGGAAATCCCACTAGCTTGCAGGGATGTTGTGATTGAATACTCGAAG CATGTAATGGAATTAGGAGGTTTACTCTTCCAACTTCTCTCAGAAGCTTTAGGTTTAGACTCTGAGATTCTTAAGAACAT GGATTGTCTCAAGGGTTTGCTTATGCTCTGCCATTATTATCCACCTTGTCCACAACCTGACCTAACTTTGGGCATAAGTA AACACACCGACAATTCCTTCATAACAATTCTTCTTCAAGATCAAATCGGTGGTCTTCAAGTTCTTCATCAAGATTCTTGG GTTGATGTAACTCCTGTTCCTGGAGCTCTTGTCATCAGTATCGGTGATTTCATGCAGCTGATCACAAACGATAAGTTCTT AAGTATGGAGCATAGGGTACGGGCAAACAGAGATGGACCGCGGATTTCAGTTGCTTGCTTCGTTAGCTCGGGAGTGTTTC CAAATTCCACTGTTTATGGACCGATAAAAGAGCTTCTTTCTGATGAAAACCCTGCAAAGTACAGAGACATCACTATACCA GAATACACTGTAGGATACCTAGCAAGCATCTTCGATGGAAAATCGCATTTGTCTAAGTTCCGGATATGAAGAAATTACTT GTATGGCTTAGTTAAAAACAGATATGTGTGTTTTGCTGCTCTCTTCTCGCTCGAGAGGACATCAACTATTTTGTTAATAA GAAAGAAT .......................i..................i..................................... .................N.............................................i................ ................................................................................ ...........i.................................................................... ................................................................................ ........................................................N....................... ...............................................................N................ .....................................N.......................................... .......................................................i........................ .........................................................N...................... ......................N......................................................... ................................................................................ ....N..............................................N............................ ....N...........................i............................................... ...............N..........................N..................................... ..................................N..............................N.............. ..N....................N........................................................ ........ Pos Score Pred ------------------------ 24 0.610 Yes 43 0.688 Yes 98 0.325 - 144 0.711 Yes 252 0.747 Yes 457 0.280 - 544 0.258 - 598 0.109 - 696 0.640 Yes 778 0.274 - 802 0.433 - 807 0.314 - 879 0.356 - 903 0.393 - 1045 0.110 - 1092 0.319 - 1125 0.480 - 1153 0.589 Yes 1216 0.390 - 1243 0.381 - 1315 0.310 - 1346 0.217 - 1363 0.193 - 1384 0.122 -

Explain the output -- Processing locus 532: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACATAGAGAGATAGCATAAGAAAACAGAGAGGAGACCTATGCACAGATCTGAGATCTCAGTGGATGAAGTCTTTGTTTAT ACATAGAGAGATACAATATAAGAAACAGAGAGAGGCCTATGCACAGTCACAGATCTCTCAATTAAATTCGCAATGGAGTT AGAAAAAGTTCACGTGGTTTTGTTCCCATACTTGTCCAAAGGGCACATGATTCCTATGCTCCAATTAGCTCGTCTCCTCT TATCCCACTCCTTCGCCGGAGACATCTCCGTCACCGTCTTCACCACTCCTTTGAACCGTCCTTTCATCGTTGACTCACTC TCCGGCACCAAAGCGACCATCGTCGACGTACCTTTCCCTGATAACGTCCCGGAGATCCCACCCGGCGTCGAGTGCACTGA CAAACTCCCTGCTTTGTCGTCCTCCCTCTTCGTTCCTTTCACAAGAGCCACCAAGTCAATGCAGGCAGACTTTGAGCGAG AGCTCATGTCACTGCCACGTGTCAGTTTCATGGTCTCAGACGGTTTCTTGTGGTGGACGCAAGAGTCAGCTCGAAAGCTA GGGTTTCCTCGGCTTGTTTTCTTTGGTATGAATTGCGCTTCCACCGTTATATGTGACAGTGTTTTTCAAAACCAGCTTCT ATCTAATGTTAAGTCCGAGACGGAGCCAGTTTCTGTACCGGAGTTTCCGTGGATTAAGGTTAGGAAATGTGATTTCGTTA AAGATATGTTTGATCCAAAAACCACCACAGATCCTGGATTCAAGCTTATCCTAGATCAAGTCACGTCTATGAATCAAAGC CAAGGTATCATATTCAATACATTTGACGACCTTGAACCCGTGTTTATTGATTTCTACAAGCGTAAACGCAAACTCAAGCT TTGGGCAGTTGGACCGCTTTGTTACGTAAATAACTTCTTGGATGATGAAGTAGAAGAGAAGGTCAAACCTAGTTGGATGA AATGGCTAGATGAAAAGCGAGACAAGGGATGCAATGTTCTGTATGTGGCTTTCGGGTCACAAGCCGAGATCTCGAGAGAA CAACTAGAGGAGATTGCGTTAGGGTTGGAAGAATCGAAGGTGAACTTCTTGTGGGTGGTCAAAGGAAATGAAATAGGAAA AGGGTTTGAAGAGAGAGTGGGAGAAAGAGGAATGATGGTGAGAGATGAATGGGTTGATCAGAGGAAGATATTAGAGCACG AGAGTGTTAGAGGGTTCTTGAGCCATTGTGGGTGGAATTCTCTGACGGAGAGCATTTGCTCGGAGGTTCCAATCTTGGCG TTTCCTTTAGCAGCGGAGCAACCTCTGAATGCGATTTTGGTGGTGGAAGAGCTGAGAGTGGCGGAGAGAGTGGTGGCGGC GAGTGAAGGGGTTGTGAGAAGAGAAGAGATTGCAGAGAAAGTGAAGGAGTTGATGGAGGGAGAGAAAGGGAAAGAGCTGA GGAGGAATGTCGAGGCATATGGTAAGATGGCGAAGAAGGCTTTGGAGGAAGGTATTGGTTCGTCTAGGAAGAATTTAGAC AACCTTATCAACGAGTTTTGTAACAATGGAACATGATGATGTATGTTAACACCTATATATTAATACTAGATTTTAACCCG CGGTATATCGCGGGACGATTTATTTTTTAAAGTAATATATATTAAAATTTGCAAA ......................................N........................N................ ......................................i.................................N....... ..............................................N........N........................ ................................................................................ ................................................................................ ..........................................................N..................... .....N.......................N.................................................. ...........................N......................N............................. .....N............................................................i............. .....N..............................................................N........... ................................................................................ .........................................N..N...............................N... ...................................................................N............ ...............................N..i.........N...i............................... ................................................................................ ............................N................................................... ....................................................i........................... ......N...........N.......N..................................................... .........................N......N..N..N...N..................................... ....................................................... Pos Score Pred ------------------------ 39 0.452 - 64 0.295 - 119 0.528 Yes 153 0.446 - 207 0.293 - 216 0.419 - 459 0.463 - 486 0.202 - 510 0.264 - 588 0.171 - 611 0.422 - 646 0.264 - 707 0.646 Yes 726 0.496 - 789 0.224 - 922 0.423 - 925 0.338 - 957 0.496 - 962 0.530 Yes 970 0.272 - 989 0.357 - 994 0.329 - 1003 0.133 - 1108 0.380 - 1152 0.434 - 1155 0.594 Yes 1165 0.436 - 1169 0.551 Yes 1309 0.132 - 1413 0.579 Yes 1447 0.226 - 1459 0.292 - 1467 0.350 - 1546 0.233 - 1553 0.393 - 1556 0.121 - 1559 0.310 - 1563 0.166 -

Explain the output -- Processing locus 533: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGTTTCTGGTTTTTATAGGACTGTTGGGTATTTTCTATGTTCTTCGTATAAAACTCATCAAAAGTTTTGAGAGATATTT TCCTATATTATTTGCTAGTAAGAGAGGTTTTCAGACGAAGAAAAAGAAAATCTTCATAAAATGAGTTGTTTCTCGTGCTT CTCTTCAAAAGTTCTGGACAATGAAGGTTCTTCGATGCCTGCTCCTTATAAGCAACCAAACTCACCTAAGAGAACAACAG GAGAAGTGGTGGCTAAGAACGCAAACGGACCATCAAACAATATGGGGGCACGTATTTTCACGTTCCGTGAGCTAGCCACC GCGACAAAGAATTTTAGACAAGAGTGCCTCATTGGTGAAGGTGGGTTCGGGAGGGTTTACAAAGGAAAGCTTGAAAATCC AGCTCAGGTCGTGGCAGTGAAACAACTTGATAGGAATGGATTACAAGGACAAAGAGAGTTCTTGGTCGAGGTCTTAATGC TTAGTCTTCTACATCATAGGAATCTTGTGAATCTTATCGGTTATTGCGCTGATGGAGACCAAAGACTTCTTGTTTACGAG TATATGCCTCTTGGATCTCTAGAAGATCATCTCCTCGATCTTGAACCGGGACAAAAACCACTAGACTGGAACACTCGTAT TAAGATTGCTTTAGGAGCAGCAAAAGGAATTGAGTATCTCCATGATGAGGCTGATCCTCCAGTGATTTACCGAGATCTTA AATCGTCAAATATATTACTTGACCCAGAATACGTTGCGAAATTATCGGATTTCGGTTTAGCAAAGCTAGGTCCTGTTGGA GACACACTACATGTGTCTTCCCGAGTTATGGGAACTTATGGTTATTGTGCTCCTGAATATCAAAGAACTGGCTATCTAAC AAATAAATCAGATGTCTACAGCTTTGGAGTTGTGTTGTTGGAACTTATATCCGGAAGACGAGTCATCGACACAATGAGAC CTAGTCATGAACAAAATCTCGTTACTTGGGCACTACCGATTTTTAGAGATCCGACTAGATATTGGCAGCTAGCTGATCCA TTGCTTCGAGGCGACTATCCGGAAAAGAGTTTGAACCAAGCTATAGCTGTAGCGGCAATGTGCCTGCATGAAGAACCAAC GGTTAGACCACTAATGAGTGACGTTATTACAGCCTTGAGCTTTCTTGGTGCATCCTCAAATTCCTCCAATACCGGCTCTA ACCATTTGCAACAAAATCGATCCAACAAATATCAGGATGCAGTTCAGTGGGACTCAAGTCCTAGATATGCTAATTCGCAA ATGTAGACATTTTTATATTTTATATGACCCAACAATAATTCACAACTTATGTATCTTCCCTTTATTTTTACTTTTGGGTT ATAGTTTCATCTATTTATAATGAAT .....................................N.......................................... ............................................................i................... ....................N.............i............................................. .........................................i...................................... ................................................................................ ...................................N........................................N... ...................................................i............................ ...N............................................................................ .........................................N..N................................... ................................................................................ ..........N................N.........N.......................................... ...........N.............................................................N...... ......N......................................................................... .........................................................N.........N............ .............N.................................................................. ....................................N.............................N............. N......................N........................N............................... ...................N..... Pos Score Pred ------------------------ 38 0.249 - 141 0.753 Yes 181 0.446 - 195 0.817 Yes 282 0.808 Yes 436 0.375 - 477 0.312 - 532 0.549 Yes 564 0.395 - 682 0.175 - 685 0.157 - 811 0.288 - 828 0.333 - 838 0.431 - 892 0.348 - 954 0.281 - 967 0.126 - 1098 0.354 - 1108 0.183 - 1134 0.484 - 1237 0.300 - 1267 0.136 - 1281 0.285 - 1304 0.058 - 1329 0.060 - 1380 0.067 -

Explain the output -- Processing locus 534: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACTACAGTAAAAGTAAAGGCTTTATAGGGTCAACAGAACGACACTAGCTACATGCATAATGTGAGTTTGATTTCCTGTC TGGGACCTCACTCAAGCATAAACCAATGTTAACAATCCACAGAACAAACATATCAAACAAAGTGACTATGACATTTTCTT TGGAATGCAGAGCCTTAAGGTGGTACCTTTCCTGTATAATGTAACTGTGGCTTGCTATGGCTATCCACATATGTCTTTGG ATAAAGTTTTGAAGACGAGGTTGTTGAAAATACAAGGGAATCACAAAGGCTTTAACTTGCTTAATCCAGCATTTGTAGAC TTTGATATTTGTTTAGATCTTATCACAAAGGAAATTCCAACTTTCGCCAATCAGAAGGCTATATTTCGTACCCACTCGCA GAAAGAGCGCGTTGAGCGAGTGAGGGCGATTCTAAGTGGAAGATCAATCAAAAATAATAGAAGAACAGAGACGACGAAAC CTGCCGGAGAACTGAGGGGAGAAAAAAACCCGGCGAAAAAGGGAAATTCGAATTCGTCTTCTGTTGATCATTGCTTCACT TCTGCTTCCAGAGCTTTTACTCAGAAGAAGCTCGCTGATCTCAAATCTCTCTTTTTTTCCCTCGCTTCCAAGTCTCAGAG CAATGACCAATACGTCTCGTACCCTGTTTTCCAGGAGTATTTTGGTCTGAGTGGTTCTTTAGGAGAAAGGATCTTTGATA TGGTTACTCAACACAGGAAAGATGATAAAATGACTTTTGAAGATCTTGTTATTGCTAAGGCAACATACGAGAAAGGAACT GATGATGAGATTGCTGAGTTTATTTACCAGACTTTGGATGTTAATGGCAATGGGGTCTTGAGAAGGTCTGATTTAGAGTC GTTCCTGGTGGTGATTTTGAAGAGTGTGTTCTCCACTGAGAGTTCTGATGCGGAATCAAGTGATTACAAGGAGATGGTAG ATGCGCTACTCGATGCCGCTACTTTCTCAAAATCTGATGATGGTTCTGAAAAAGGAATGTCTTTTGAAGACTTCAGAAGC TGGTGCCCACTCGTTCCAACTATCAGAAAGTTCCTCGGCAGCTTGCTTATTCCCCCGGGTCCAGTGAGACCTGGATATCA AGTCCCGGATCTGCTATATGAAGATAGTGTGAGTTCAGATAGGCTACTATTAAAGAAGGAATACGCTTGGCATATTGGAG GAGCTCTTCCTCACCACGAGCTTGTAGAGTGGAAGCTGTTGTATCGCAGTTCCTTACACGGTCAAAGCTTCAACACATTC CTCGGACACACATCGGGGATGGAGGGATCGAACATGGCCTGATGGATGGACTGCAGTTACCGCAGATGGAAAACGCAGCG CTCAGTTTGAGCATACCCTATTGGTAACTGAGACTGGTGTTGAGGTTTTAACAGCGAGGCT ....................................................N......N.................... .........................N.........................................N............ ....N.................................N.................N.............N......... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ..i............................................................................i .....................N.......i.................................................. ...............................................N.........................i...... N...........N.......................N..N................i....................... ................................................................................ .................N.............................................................. ................................................................................ ..................N..............i.......N...N...................N.............. ............................................................. Pos Score Pred ------------------------ 53 0.378 - 60 0.452 - 106 0.167 - 148 0.177 - 165 0.371 - 199 0.156 - 217 0.185 - 231 0.373 - 643 0.516 Yes 720 0.838 Yes 742 0.141 - 750 0.910 Yes 802 0.135 - 805 0.137 - 838 0.058 - 844 0.084 - 850 0.411 - 928 0.231 - 954 0.614 Yes 961 0.255 - 973 0.251 - 997 0.311 - 1000 0.345 - 1017 0.590 Yes 1138 0.214 - 1299 0.399 - 1314 0.538 Yes 1322 0.192 - 1326 0.260 - 1346 0.061 -

Explain the output -- Processing locus 535: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAGTTCCTTATATAAGTTAATGTTAATTTAGTTGTCGGCACATATACCTTTTAAATTATCGAATATAAAGCCATTGAT AAATCTTGATTCTGATGTTGAATGAGAAAAAACTGGTCACATTATATTGGACCCACCAATCTTAAAGTGACTAGGCCTAC AAAAATTAAATCTATCAAAAACACAAATGTCTCTCTATATATAGTTACACAACTTCTTTGGACTTCTTCACATTCACTCA TATCACTACACTTTCCATTAATTACCAATATATTTTTACTACTTAAAAAAGATATTGGTCTTGAAATGGAGAGTTACTTA ACGAAATGGTGTGTAGTGCTTGTGTTGTTGTGTTTCGGGTTTAGTGTAGTAAAAGCACAAGCACAAGCTCAAGTTCCATG TTTCTTTGTTTTTGGTGACTCTTTGGTTGACAATGGAAACAACAACGGTCTTATTTCTATTGCAAGATCCAATTACTTCC CTTACGGTATCGATTTCGGCGGCCCTACCGGCCGTTTCTCCAACGGCAAGACTACTGTTGATGTGATCGCTGAGCTACTT GGATTTAATGGCTACATTCCTGCGTACAATACTGTGAGTGGTCGGCAAATACTCTCCGGAGTTAACTACGCTTCCGCAGC TGCTGGAATCCGAGAAGAAACCGGTCGACAATTGGGACAAAGGATAAGCTTTAGTGGACAAGTTAGGAACTACCAGACCA CAGTATCGCAAGTTGTGCAGCTTCTAGGAGACGAGACTCGTGCAGCTGATTACCTCAAGAGATGTATTTACTCTGTTGGT TTAGGAAGCAACGACTATCTCAACAACTACTTCATGCCTACGTTTTACTCTTCTAGCAGACAATTCACACCCGAACAATA CGCTAACGATCTCATCAGTCGCTACAGCACCCAGCTCAATGCATTATACAACTATGGGGCTCGAAAGTTTGCATTGAGTG GAATTGGAGCAGTTGGTTGTAGTCCTAACGCGCTCGCAGGCAGTCCCGACGGTAGGACTTGCGTAGACCGCATCAACTCA GCAAACCAGATCTTCAACAACAAGCTAAGATCTCTTGTTGATCAGCTCAACAACAATCACCCTGATGCAAAGTTCATCTA CATCAATGCTTATGGCATTTTCCAGGACATGATCACAAATCCTGCCAGATTTGGGTTTAGAGTGACGAACGCGGGATGTT GCGGTATTGGGAGGAATGCTGGACAGATCACATGTTTACCGGGACAGAGACCGTGTAGAGACCGCAATGCGTACGTGTTC TGGGACGCATTTCACCCTACTGAAGCCGCAAATGTGATCATCGCAAGGAGATCGTACAATGCACAATCGGCTTCAGACGC TTACCCTATGGATATTTCAAGGCTGGCACAGCTTTGAAGAAACATAGTAAAATAAGAACTAAAGAAGTTCCACAAACCAA TGTGTGATCTTGTTGTGTACCGTACCGCTATTTAAGATTTTCTTGACGTAATTGAA .....................N.......................................................... ..............N......N.......................................................... ..........................i..................................................... .................................................................N.............. .....N.......................................................................N.. ................................N............................................... ............................................................N................... .......i........................................................................ ................................................................................ .............................................................N.................. .................................i.............................................. ......................................i..............i.......................... ................................................................................ ................................................................N............... .....N.....i................i..............................................N.... ...............N...............i..................................N............. ...............................N..........................i..................... .......N.......................................................................N ........................................................ Pos Score Pred ------------------------ 22 0.345 - 95 0.317 - 102 0.165 - 187 0.561 Yes 306 0.350 - 326 0.289 - 398 0.111 - 433 0.469 - 541 0.242 - 568 0.606 Yes 782 0.279 - 834 0.549 Yes 919 0.533 Yes 934 0.722 Yes 1105 0.287 - 1126 0.274 - 1132 0.510 Yes 1149 0.694 Yes 1196 0.379 - 1216 0.396 - 1232 0.504 Yes 1267 0.401 - 1312 0.256 - 1339 0.757 Yes 1368 0.323 - 1440 0.216 -

Explain the output -- Processing locus 536: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACACATGTGACTAGTTTGACCTTTAGGAGCGCCAACGTGTCATATATCAGCGTAAATTCCAAAACAAAACAATCTAGTGT GGGACCCAAATACTGTTATAAAAAAGGATCATTAAAATGGATATGTTAAAAAAAGAAAGTAAAAAACGCGTCAGGGAAGA GAAGATGGATCCGAATTCCACGGTTTCCGGCGATGGTCAGGCGACGGCGGCGATAGAGCATCGGTCCTTCGCTCGGATCG GATTTCTCGGAAACCCGAGCGATGTATACTTCGGGCGAACCATATCATTGACCATCGGAAACTTCTGGGCATCCGTGAAG CTGGAGCCATCGGAGCATCTCGTAATCAAGCCTCATCCATTCCATGATCTCGTCCAGTTCACCTCTCTCGACCATCTCCT GAATCGTTTGCAAAATGAAGGATACTACGGTGGGGTAAGGTTGCTAATGGCGATATGTAAAGTATTCCGTAACTATTGCA AAGAGAATGACATTCAACTTCACCAAGCCAACTTCTCTCTTTCTTATGATACCAATATCCCTAGGCAGACAGGGCTTTCG GGTTCTAGTGCCATCGTATCCGCTGCCCTTAACTGCCTTCTCGACTTCTACAATGTCAGGCATTTGATCAAAGTACAAGT CCGCCCTAACATTGTTCTCAGTGCTGAGAAAGAACTTGGCATTGTTGCTGGTCTTCAGGACAGGGTTGCTCAGGTCTATG GTGGTCTTGTTCACATGGATTTTAGCAAGGAGCACATGGATAAATTGGGGCATGGGATTTACACTCCTATGGATATCAGT CTCCTCCCTCCTCTGCATCTCATCTATGCTGAGAATCCGAGCGACTCAGGGAAGGTACATAGTATGGTTCGGCAAAGATG GTTAGACGGTGATGAGTTTATAATCTCATCAATGAAAGAAGTCGGAAGTCTAGCAGAAGAAGGTCGAACTGCATTACTCA ACAAGGACCATTCCAAACTTGTGGAACTCATGAACCTTAATTTCGACATTCGGAGGCGGATGTTTGGGGATGAATGCTTA GGAGCAATGAACATAGAGATGGTGGAAGTAGCAAGGAGGGTTGGTGCAGCCTCAAAGTTCACTGGAAGTGGAGGAGCAGT GGTGGTTTTCTGCCCTGAAGGTCCATCTCAGGTGAAACTTCTGGAAGAAGAATGCAGGAAAGCGGGATTTACGCTTCAGC CGGTAAAAATTGCGCCTTCATGTTTGAATGATTCTGACATTCAGACCTTATGAAAGCCTTGATCAAGTCTTCTTATTCAC ACGATAGAGCCATTTTCCAGAGAAATAAACATGTGAGCTTTAATTGGGTGGTTGTTCTGTTTCTTTTTCTTG ....i........................................................................... ....................................i.....N..................................... ....i...........................N............................................... .....................N.......................................................... ...........................................N.................................... ..............N...............................i.......N......................... ......N......................................N.................................. ....................................................N........................... .............................................................................N.. ..............i....................i...............N................N........... .........................N.....................................i.............N.. ...........N...................i................................................ .............................N.............................i.........N...N...... ......N...........i............................................................. ...................................................N............................ ...................N.......N.....................N.............................. ..............................N......................................... Pos Score Pred ------------------------ 5 0.531 Yes 117 0.876 Yes 123 0.465 - 165 0.594 Yes 193 0.285 - 262 0.280 - 364 0.345 - 415 0.331 - 447 0.592 Yes 455 0.445 - 487 0.294 - 526 0.104 - 613 0.367 - 718 0.057 - 735 0.551 Yes 756 0.804 Yes 772 0.186 - 789 0.469 - 826 0.350 - 864 0.712 Yes 878 0.492 - 892 0.307 - 912 0.529 Yes 990 0.450 - 1020 0.610 Yes 1030 0.215 - 1034 0.424 - 1047 0.447 - 1059 0.821 Yes 1172 0.465 - 1220 0.325 - 1228 0.109 - 1250 0.219 - 1311 0.061 -

Explain the output -- Processing locus 537: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCACAGAAGACAAGGAAAACTAAACCATAGTGCAAAGAAAACTAAATTGGTCTTCTTTCAATCAAGAGATATTCGAAAGG TAAATTTCTTATGAGTTTTGAATTACTTTTTTGCATCTATGTCTAAAGCTAACAACTGCAAAACATTTCGATGGCACAAA GGAAAGAAGAATGGGTTCATGCAGTCCTCAACTACCACTCATCTGTCTCTCGGACCAAACCCTAAAACCAGGAAGCTCAA AATGGGTTAAAGTGAGGAGTGATGTCCGTAAAGCTTTAGAAGACTACGGTTGTTTCGAAGCCAAAATTGATCAAGTGTCA ATGGAGCTTCAGGGGTCGGTTTTGAAGGCCATGCAAGAGCTTTTTGCGTTACCGACAGAGGCCAAACAAAGAAATGTGTG CCCGAAACCTTTCACTGGATATTTGTCTCATAATGGTCTCTCCGAGAGTTTTGGGATCAAGGATGCTAATATTTTGGAGA AAGCTCACGAATTTACTCAACAACTATGGCCTGAGGGTAACAAGAGTATCAGTAAGATGATCCAACTGTATGCTGAGAAA TTAGCAGAACTGGATATGATGGTGAGAAGATTAATACTGGAGAGCTATGGGATAGAATACTTCATTGATGAACATTTGAA CTCAACGTATTACCGTATGCGATTGATGAAGTATATAGCACGGCCTGATAATGATATAACTGCTGCTGTTGGTGCTAATG TGGATAATGGTGCTAACGATAATGCCGATGGTGATGCTAATGTCAACGATGATGGTGCTAGTATTGGTGTTAAAGTTAAT GTCGATGTTGGTGATGATGTTAATGACAATGATAGTGTTAATATTGGTGTTGGTGTTGATATTAACGTTGAGACCAATGT TAATGGTGATCTTGATGCTGAAGCTAATGGTGATGCTACTGCTTGGGTCGTTGGTGCTGTTAGTGGGAATGCTAGCGTTG GTGCCAAAGAAGCTAATGTTGATGCGGAGTTGGGCTTACCTAGTCATACTGATAAAAGCCTTACTGGAATAATTTATCAG CATCAAATTGATGGTTTGGAGGTGAAGACCAAAGAGGGGAAGTGGATCAGAGTTAAACCAGCTCCTAATACCGTCATTGT TATTGCGGGAGATGCTCTATGTGCACTTATGAATGGTAGAATCCCTTCACCGTATCACCGAGTAAGGGTAACAGAGAAAA AGAAGACGAGATACGCAGCAGCATTGTTTTCGAATCCAAAAGAAGGTTATATCATAGATTCACCAAAAGAACTTGTGGAC GAGAAGCATCCACGTGCCTTCAAACCCTTTGATTTTGTCGACCTTTTTAACTTCTATCACACAGAAGCTGGTCGTAGAGC TCCATCTACTCTCCAAGCTTTTTGTGGCGTCTCTGCTGGGAAATAAAGGTGTGCGCTTCAAAATGTTTGGACGTGGTTTC AAGTTTCAACTAGTATCAACAACTATCTTACGAAATAAGGTGTTTATGTTGTGTAATAAACATTT ................................................................................ ..........N...........................i...............................i......... ..........i.......N............................................................. i.............................N..........................................N...... ................................N.............................N................. .........................i..............................N............N.......... ...............N..N...........................N....................N............ ................i........i........................N..........................N.. ......N..............N.....N.....N.....N........N..N..........................N. ....N........N..N.....N.....N...............................................N... ..N...........N...........N.....N...................................N........... ...............N.....N.......................................................... ..........N..................................................................... ...........N......i.........N...N............................................... ................................................................................ ................................................................................ ..............................................................N................. .............................................N................... Pos Score Pred ------------------------ 91 0.366 - 119 0.502 Yes 151 0.790 Yes 171 0.688 Yes 179 0.478 - 242 0.628 Yes 262 0.510 Yes 321 0.504 Yes 351 0.301 - 394 0.325 - 433 0.327 - 463 0.126 - 506 0.802 Yes 537 0.199 - 550 0.075 - 576 0.295 - 579 0.427 - 607 0.296 - 628 0.121 - 657 0.914 Yes 666 0.803 Yes 691 0.179 - 718 0.129 - 727 0.105 - 742 0.106 - 748 0.120 - 754 0.075 - 760 0.056 - 769 0.090 - 772 0.039 - 799 0.127 - 805 0.045 - 814 0.046 - 817 0.076 - 823 0.082 - 829 0.080 - 877 0.105 - 883 0.110 - 895 0.071 - 907 0.109 - 913 0.068 - 949 0.084 - 976 0.159 - 982 0.128 - 1051 0.071 - 1132 0.109 - 1139 0.726 Yes 1149 0.374 - 1153 0.234 - 1423 0.127 - 1486 0.053 -

Explain the output -- Processing locus 538: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTATTCATAATCATTAATTATAAACAACTATAGTGGCCCACTTGAAGAAATTATGTAACATTTATTTCTTCCTTAATCA TCCATTTTCTTTATTGTGTAATTGATAATTATTGTTTTGTATATTAAATCACAAGTCACCACCACACACTTTAGTATAAA AGCAATCCTGATGTGCTATCAAACTTCAAAAAAAAAAAGAAGAAGTAGGAAAAGAAGAGCTCAAGAAGAAAAAGAGAAAA TGGGGAAAGGAGTAGTATCAGAGAAGGTGAAGCTCGTGGTGGCATTGATCACACTTCAGTTTTGTTTCGCAGGCTTCCAC ATTGTCTCAAGAGTTGCTCTTAACATTGGTGTCAGCAAAGTTGTGTACCCTGTTTATAGGAATCTCCTTGCCCTTCTCCT CATAGGTCCCTTCGCTTACTTCTTCGAAAAAAAAGAAAGACCTCCACTTACAATCTCTTTACTGGCTCAGTTCTTCTTCT TGGCACTCATAGGCATCACAGCGAACCAAGGATTCTACCTATTAGGACTTTACTATGCAACTCCAACTTTTGCTTCCGCA ATGCAGAACTCTGTTCCTGCCATCACTTTCATCATGGCTTGTGCCTTAAGGCTAGAGCACATAGACCTGGTCAGGAAACA TGGTGTGGCAAAAGTATTGGGAACTCTTGTGAGCATCGGTGGAGCCACCGTGATCACATTGTACAGAGGATTTCCGATAT TTGACCAGGGCCTTAACATGCAAAAGGAAGAGGTGGTCGGATCTGACAACTCGCACAGCTTGACACTCGGATGGTTATAC CTTATGGGACATTGTCTGTCATGGGCTGGTTGGATGGTTCTTCAAGCTCCTGTTCTAAAGCAATACCCGGCGAAGCTCAC TTTAACATCCTTCACATGTTTCTTTGGTCTGATTCAGTTTCTGGTTATTGCTCTCTTTGTGGAAACTGATCTAAATAATT GGATCATCGTCTCTTGGGAAGAGCTCTTCACCATCCTATACGCCGGAATAATAGCGTCAGGGTTGGTGGTTTATCTTCAA ACATGGTGTATCTACAAAAGCGGTCCAGTTTTTGTCGCTGTTTTCCAGCCTCTCCAGACACTTCTTGTTGCTGCGATGGC GTTTCTTATTCTTGGTGATCAGTTATACTCGGGAGGGATTGTTGGTGCAGTGTTTATAATGCTAGGGCTCTACTTAGTTC TTTGGGGCAAAAACGAAGAAAGAAAACTGGCTCTTGAAGAGAGTCAACAAGATCCAGAGTCACTGACCAAACATCTACTT GAGGCACAACACAAGAAGAGTAATTCTGAATCTGAAGTGTAAAAGTGCTGCAAACACAACGCATTACTTCTCTGTACATA CACATACAAGAACTTGGCCAACACACAGGCTCCTCAAGTGGGTCTCTCATACAATTGATAT .....................................................N.......................... ................................................................................ ..........i....................................................................i ................................................................................ ................................................................................ ................................................................................ ......................................................N......................... i................................i.............................................i ................................................................................ .................N....................................................N......... ...N................N............N.............................................. ...............N................................................................ ................................................................................ ..i........................................................................i.... ..........................................................N..................... ................................................................................ ................................................................................ ............................................................. Pos Score Pred ------------------------ 54 0.238 - 171 0.558 Yes 240 0.648 Yes 535 0.351 - 561 0.603 Yes 594 0.815 Yes 640 0.817 Yes 738 0.261 - 791 0.453 - 804 0.220 - 821 0.362 - 834 0.300 - 896 0.313 - 1043 0.522 Yes 1116 0.614 Yes 1179 0.445 -

Explain the output -- Processing locus 539: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTCCTAAAACTCTTCAAAATCTCTGTTTTTTTTCCCACTGAATCCACATTCAAGCTCATTGTAGCTTGTGTTGTGTTAT TGCAATCTGATAATCATGTAAATGTGGGTTTTCTTTGTTTTGAAAAGGGCGAAAGGAAAATGTGTAGCGGATAAGCGAGC CAAGAGGCTTACTTTGGGATCCCATGTGGCTTCGCCATCTTCAATGAGCTTCAGAGTTTCAGCTAGTAGTTCTGTTAAAC CTGAAAAGGATATTAGGATTGGTCTTCTTGGTGCAAGTGGCTACACTGGTGCTGAGATCGTTAGGCTTCTTGCAAATCAT CCGCATTTCCAGGTCACTTTGATGACTGCTGATAGAAAAGCTGGCCAGTCAATGGAAAGCGTTTTCCCACACCTGAGAGC TCAAAAACTACCTACTTTGGTCTCGGTAAAGGATGCAGATTTTTCTACTGTGGATGCTGTATTCTGCTGTTTACCTCACG GAACAACTCAGGAAATCATCAAGGAACTGCCTACTGCATTGAAAATCGTTGATCTTTCAGCGGACTTCCGGTTGCGTAAT ATTGCAGAATATGAAGAATGGTATGGTCAGCCTCACAAGGCAGTAGAGTTACAGAAAGAAGTTGTGTATGGTCTAACAGA GATACTAAGGGAGGACATAAAAAAGGCACGACTTGTGGCTAACCCAGGCTGTTACCCGACTACGATTCAACTTCCTCTTG TTCCTTTACTAAAGGCAAATCTCATCAAACATGAAAACATCATTATCGATGCAAAATCTGGTGTTAGTGGAGCAGGACGT GGTGCTAAGGAGGCAAATCTTTACTCTGAGATAGCTGAAGGCATTTCTTCTTATGGTGTCACCCGTCATCGCCATGTTCC TGAAATTGAACAGGGATTATCTGATGTTGCACAGTCAAAAGTAACAGTCAGTTTTACGCCACATCTCATGCCAATGATCC GTGGAATGCAATCGACTATATATGTGGAAATGGCTCCCGGGGTTAGAACCGAAGATTTACACCAGCAATTGAAGACGTCT TATGAGGATGAAGAATTTGTCAAAGTGTTGGATGAAGGAGTTGTTCCTCGGACACACAACGTTAGAGGATCCAACTATTG TCATATGAGTGTCTTTCCTGATCGAATTCCTGGAAGAGCTATCATAATCTCAGTGATTGATAATCTTGTGAAAGGAGCTT CGGGACAAGCGTTGCAGAATCTTAACATAATGTTGGGATATCCCGAAACAACGGGGCTCCTACACCAGCCGCTTTTCCCT TAAAAATCCCACCTTTAAATCGGTAAAATAGCATTCTTCTCTCTTGTTGGCTCTGTATCTTGAAGATATTTCTGTCATTA GATTTGCCTGCTTAAGCGTTTG ................................................................................ ...............N.....i.....................................i.................... .......................N...................N.................................... ................................................................................ .....................i.............................i............................ ................................N....................N.......................... ................................................................................ ..........N......i....N............................................N............ ................................................................................ ..............................N.................N............................... ....................................................N....................N...... .......................N...........................................i.....N...... .....i...............N.......i.................................................. ....i........................................................................... .............................N.................................................. ................................................................................ ...................... Pos Score Pred ------------------------ 96 0.325 - 102 0.588 Yes 140 0.668 Yes 184 0.240 - 204 0.378 - 342 0.594 Yes 372 0.538 Yes 433 0.090 - 454 0.229 - 571 0.301 - 578 0.614 Yes 583 0.169 - 628 0.173 - 751 0.319 - 769 0.352 - 853 0.206 - 874 0.164 - 904 0.112 - 948 0.525 Yes 954 0.360 - 966 0.597 Yes 982 0.257 - 990 0.778 Yes 1042 0.261 - 1048 0.162 - 1072 0.290 - 1125 0.680 Yes 1230 0.250 -

Explain the output -- Processing locus 540: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 541: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATAGTTGTTGCCAAAATTATGTATTTTATTACCCACTTTAGATACCTCTAATTTTACATAAGTTTCAATATACCTTATA TAGTTGACTCTATTGCTCTAGAATTGTAATTATTTTTTATTTTGCAATAAGTTCCAATTTCAGCATTATTTTTCACCGGG AAAATCCTTAAAATATTCTATGCAAAATCCAATATATTCACTCCACATAGATAATAATAAAAAACTTATAAAAGAAATAA TTGAAGAAAGGCTGCCCATGTGGGCACTTCCAAATAAATCTCCAGAATCGATGCTTCAAATACATTGGATGTTTACTAAA CAACACAGATATGAACAAACTTCAACAAACAAACACACCCACAAAGGAAAATCCGAAGACCCGATCTTTGTTATAAAACA CACAACTTCTCTCTTTTTAGAATCTCTTGTCTTCTTCTTTTTAACTTTCTTTTGTAATCTCTTGTCTGAAAAAGAAATGG ATCATGTAAGTTCAAGTGAGTTACTTATTGATGATCTTCTTGAGGATTACTGGTTCTTTGAGAACTTATTTACAAGAAGG TCGAGAGGCTTAAGGTATTGTCACTCAGATCCTTACCCCTCTTCTTCTTCTACTTCGACTTCTCCTGAAAAAATGGGAGA TTCAGATATTGGGAAGGTTCTAGAAGCTTCCACAGGGCGATCTTTAATCAGGGCATCTTCTATAGATAGTAGAGAAGGCG GATCTCAGACGAAGCTGACGGGCCGGTTCTCTGAAAAGATTAGGGTTCAAGAACAGAGACAAGTTGGGTCTTCCTTGCAG AAGAAGGAACATGTGGTTCTTCCTAAATCTGGATCTCGTTCGGCTCCGGGGAAGATTCAAGAAGCTTCCACAAAACGTGG TTTGATCCGGGCACCTTCTTTGCCGCCTCAGATAGAAAAACGAGAGATGGACCGCGAGGCAAAGAAGATGATCAATAAGC TGACAAGGCAGTTCTCTGAGAAGATTAGGGTTCTAGAACCGACGAGGCCTGGTGAGCATTTCTTGCAGAAAAAGGAAACT ATTGCTCGAGACAAAGGGATTACTGAAAGTTCTAGAAGCAACAAAACAGGAAGTAGCAGTAGTTATAGTAGTGTCAAGAT CAGTTTGCAAAGAACTCAAACGATGCCAAATAACATGGGAAGAGAAGAAGATAACGAGGAAGACGAGTTTGAAGATCAAG AAAGCGACTCAAGAATGGGATTCTTGATCCGTGAAGCCCTGGCTAGTTCACATTATGTTCCAAAGGTTTCAAACAATCAA AGGCAGAGACCACCAAGAAGCTTGAGATTAGAAGACACTGCTATGGTCAAACAAGGTGGCTCAAGTCCCAAAACGCTGCG TAAAACGTTAAGCAGCGTAGAAACAACCAAAGAGATTCAAAGGCTGAAAGGTTATGACCAACTGGTCGAGCCACGTGTCG CAAGTGGATTAGCTACACCACCTAGGGTTCCAAAGGATTCAAGTAAAGAAATGAAGGATCAGATCAAGTTTTGGGCTCGA GCCGTAGCTACTAATGTCCGACAAGAGTGCTAAGATTTTTCTTACACTAATTAACTGTATTTCGTACAGATGATTCTTAT CTTGAACTCTTTTGTGTGTTTTGTTTTTCTGATCCTCCTGAAATAATGAGAT ...................N............................................................ ................................................................................ ...................i............................................................ .................N................................N.................N........... ..........N..................................................................... ............................................................................i... ...i..........................N................................................. ........................................................................i....... ................................................................................ ................................................................................ ..........N..................................................................... ..............................................i....................i............ ................................................................................ ................................................................................ ......................i...........i............................................. ..............N.......................................N......................... ..........................................i..................................... .....................................................N.......................... ..................................................i............................. .............N.......................................................N.......... .............................................N...... Pos Score Pred ------------------------ 20 0.196 - 180 0.652 Yes 258 0.447 - 291 0.206 - 309 0.297 - 331 0.167 - 477 0.532 Yes 484 0.510 Yes 511 0.443 - 633 0.710 Yes 811 0.319 - 927 0.705 Yes 948 0.600 Yes 1143 0.564 Yes 1155 0.649 Yes 1215 0.401 - 1255 0.353 - 1323 0.688 Yes 1414 0.418 - 1491 0.775 Yes 1534 0.226 - 1590 0.079 - 1646 0.070 -

Explain the output -- Processing locus 542: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGATTTCTTAATTAGCTCTAAAAGATTAAGATCACAGGGGTCCATTGTCAAAATTCTCTTTTGATTTTGTTTTTGTAATC AAGAATAGTTTCTTTTTTTGATTCATGAAGAAATGTCTAATTTTTTCGTTGCAGAGAGAGATGGGTATATATCTAAGTAC TCCAAAAACAGACAAGTTCTCAGAAGATGGCGAAAATCATAAACTCAGATATGGTTTATCCTCTATGCAAGGTTGGCGTG CGTCCATGGAAGATGCTCATGCTGCAATACTTGATCTCGATGATAACACTTCCTTCTTGGGTGTCTATGATGGTCATGGA GGTAAAGTTGTTTCTAAGTTCTGTGCCAAGTATCTACACCAGCAGGTTCTTAGTGATGAGGCGTATGCAGCTGGAGACGT AGGGACTTCTCTTCAAAAAGCATTTTTCAGAATGGATGAGATGATGCAAGGACAAAGAGGGTGGCGAGAGTTAGCAGTAC TTGGTGACAAAATCAATAAGTTCAGTGGGATGATTGAAGGGCTTATATGGTCACCAAGAAGTGGGGACAGTGCTAATAAA CCTGATGCTTGGGCGTTTGAGGAAGGTCCTCATTCTGATTTTGCTGGACCTAATTCTGGGAGCACGGCATGCGTAGCTGT TGTTAGAGACAAGCAGCTATTTGTTGCAAATGCAGGTGACTCACGTTGTGTGATATCCAGAAAGAATCAGGCTTATAATC TTTCTAGAGATCACAAACCAGATCTTGAAGCTGAGAAAGAAAGGATATTGAAAGCTGGTGGCTTTATACATGCAGGGCGA GTCAATGGAAGCTTAAATCTATCACGAGCTATCGGTGACATGGAATTCAAGCAGAATAAGTTTTTGCCATCTGAAAAGCA AATAGTTACCGCTAGTCCAGATGTTAACACTGTTGAACTCTGTGATGATGATGATTTCCTTGTTCTTGCCTGCGATGGAA TTTGGGATTGCATGACAAGCCAACAACTCGTTGATTTCATACATGAACAATTGAATTCAGAGACCAAACTCTCGGTGGTA TGTGAAAAAGTTCTCGATAGATGTCTGGCTCCAAACACTTCAGGTGGTGAAGGCTGTGATAACATGACCATGATATTGGT TCGATTCAAGAACCCTACTCCATCAGAGACCGAACTAAAACCAGAAGCAAGCCAGGCAGAAGGAAACCACGATGAGCCGA GCTCATCAAACTAGGCCCATCCTCAGAGTCACGGAAGATCCCTCTAGATCTAGTATTTTGGTTCAGATTGTAAATCATTT TGAAAGACAGTTATATATCTCAACCTGACGTTG ................................................................................ ........................N.......N...........................i................... ..........................i.......................N.............i............... .....i......N.....N....................N..........................N..N.....N.... .......................................................N........N............... ...............................i...N....N..N.................................... .............................i................N................................. ....N...............................................................N........... .............................N.................................................. .....................................................................N.......... ....N..................................i........................................ ....................N.......................N..N..N.......................N..... ...........i..............................N....................................N ....................i..........................................i.....i.......... .......................................................................i........ ................................................................................ ................................. Pos Score Pred ------------------------ 105 0.140 - 113 0.172 - 141 0.650 Yes 187 0.700 Yes 211 0.353 - 225 0.728 Yes 246 0.703 Yes 253 0.204 - 259 0.152 - 280 0.115 - 307 0.232 - 310 0.145 - 316 0.274 - 376 0.252 - 385 0.342 - 432 0.580 Yes 436 0.225 - 441 0.354 - 444 0.439 - 510 0.529 Yes 527 0.148 - 565 0.154 - 629 0.189 - 670 0.284 - 790 0.464 - 805 0.184 - 840 0.531 Yes 901 0.383 - 925 0.437 - 928 0.242 - 931 0.265 - 955 0.079 - 972 0.589 Yes 1003 0.441 - 1040 0.312 - 1061 0.605 Yes 1104 0.653 Yes 1110 0.755 Yes 1192 0.590 Yes

Explain the output -- Processing locus 543: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGACTTAAGAGGCTATGAAACATTTGTTTGTGACCTTGTGTATACTATGATTAAGACATCAAATTGGAAACATCCGTCA AGTTGCGTCGGAAAAGTCTGAGAAACAATGGTGAATACTTGTGTGGCCGTGATTAAGTCAAGTTACCTCTCAGCTGTTAT GTCAAATGTTTTATATCCACCAAGACAGACTCCCTACTTCTCCTCTATTCCTCTTCTTACAATCTCAATCTCCATAGTCT TTGTTCTTAGTTTTCTCCTCCTCTCACTCTCTCTCTCTATGGCTTCTTCATCTTCTTCTCGAAATTGGTTATATGATGTT TTCTTGAGCTTCAGAGGGGGAGACGTCCGTGTAACATTCCGTAGCCACTTTCTCAAGGAGTTCGATCGGAAACTGATCAC TGCTTTCAGAGACAATGAGATCGAGAGAAGCCACTCTCTTTGGCCCGATCTTGAACAAGCGATCAAGGACTCCAGAATCG CAGTGGTCGTTTTCTCCAAAAACTACGCTTCATCGAGCTGGTGTCTTAACGAGTTGCTGGAGATTGTGAACTGCAATGAT AAAATTATCATACCGGTTTTCTACGGTGTGGATCCTTCCCAAGTGAGGTATCAAATCGGTGACTTTGGAAGGATCTTTGA AAAGACTTGCAAGAGACAAACAGAGGAAGTGAAAAACCAATGGAAGAAAGCGTTGACTCTTGTAGCTAATATGCTTGGAT TTGATTCTGCCAAATGGGATGACGAAGCAAAAATGATTGAAGAAATAGCCAATGATGTTTTGCGTAAACTGCTTTTAACT ACATCGAAGGATTTTGATGACTTTGTCGGCCTCGAGGATCATATTGCAAATATGAGTGCACTGTTGGATCTGGAATCTAA GGAAGTGAAGATGGTTGGTATATGGGGTTCCTCGGGGATTGGTAAGACTACCATTGCACGAGCTTTGTTTAACAACCTTT TTCGGCATTTCCAAGTTAGGAAATTCATAGACAGATCTTTTGCCTATAAGAGTAGAGAAATTCATAGTAGTGCCAATCCT GACGACCACAATATGAAGTTACATTTACAAGAGAGCTTCCTCTCTGAAATTCTAAGGATGCCGAACATAAAGATAGATGA TCCAACTGCACTGGAAGAGAGGCTAAAGTACCAAAAAGTTCTTATCATTATTGATGATTTGGATGATATAATGGTACTAG ATACATTGGTGGGTCAAACTCAATGGTTTGGATATGGGAGCAGAATCATTGTGGTTACAAATGATAAGCACTTCTTGACT GCTCATGGGATTGATCATATTTATGAAACAAGGTTATGTGGTGATGCACCGTTCGCTACAAGAAATGGGTAGAAAAATTG TTCGCATTCAGTCCATTGACAATCCTGGAGAGCGAGAATTTTTGGTGGATCCAAATGATATACATGATGTACTCAATGCA TGCACAGTAAG ...............N...............................i................................ ...........................i..................................................N. .....N.......................................................................... ......................................i.................................i..i.... ................................................................................ ..............i................................................................. ...........................................................................i.... ................................................................................ .......................................N..............................N......... .............N....N.............i..................N..N......................... ................N..................................i............................ ..........i..........N.......................................................... ................................................................................ ............i............................................N..................N... .....................................................N........N.......N......... ......................N..........N..........................N................... ....N.................N............i.......i....................i............... ......................................................N........N..N........N...N ........... Pos Score Pred ------------------------ 16 0.264 - 48 0.509 Yes 108 0.544 Yes 159 0.196 - 166 0.175 - 279 0.662 Yes 313 0.504 Yes 316 0.504 Yes 415 0.505 Yes 556 0.520 Yes 680 0.164 - 711 0.347 - 734 0.387 - 739 0.152 - 753 0.814 Yes 772 0.137 - 775 0.164 - 817 0.260 - 852 0.758 Yes 891 0.836 Yes 902 0.286 - 1053 0.659 Yes 1098 0.476 - 1117 0.161 - 1174 0.250 - 1183 0.091 - 1191 0.477 - 1223 0.216 - 1234 0.251 - 1261 0.328 - 1285 0.169 - 1303 0.124 - 1316 0.526 Yes 1324 0.513 Yes 1345 0.675 Yes 1415 0.034 - 1424 0.041 - 1427 0.049 - 1436 0.043 - 1440 0.065 -

Explain the output -- Processing locus 544: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGCGAGTGACGGAAGTGAGTTAAAGATGCTTATCCCATTGGGATTTTGATTACTTTAATAATTTTGTAGTATATATACTA AAACGATCCCTAAACTTATAACTATTTTCATGCGGGTCCTTGAATCTGCTTTTTTTGAGTAGCATGAATGAATCAACGGA CTTGTTTGTGGGAATCGTAGCAATGGAGGAGGATTGGGGAAAGACAGTATCAGAGAAGGTTATTTCGGCTTACATGTCTC TCCCGAAGAAGGGAAAACCTCAGGGACGTGAAGTCACCGTCCTCTCCGCTTTCCTCGTCTCTTCTCCTTCCCAAGACCCG AAAGTGATAGCGTTAGGGACGGGGACCAAGTGCGTTAGTGGGTCGCTGCTGAGTCCCCGTGGGGACATTGTGAACGATTC TCATGCCGAAGTTGTCGCCAGAAGAGCTCTCATCAGGTTCTTCTATTCCGAGATTCAGCGGATGCAATTGACTTCAGGTA AGAGTAATGAAGCCAAGCGTCAGAGAATTGATAGCGAAACAAGCTCAATACTAGAGTCAGCAGATTCAAGCTGTCCTGGA GAAGTCAAATATAAGCTCAAATCTGGGTGCCTCTTGCATTTGTACATTTCTCAATTGCCATGTGGTTATGCTTCTACTAG TTCACCCTTGTATGCCCTGAAAAAGATTCCATCAACACAAGTAGATGACTCATTGTTAGTTCAAGCTTCTGATATTTGCT CGTCTCGGCATTCTGATGTTCCAGAGATAGGTAGTAATAGCAACAAAGGTAATGGCTCACAAGTAGCAGATATGGTGCAA AGAAAGCCTGGTCGCGGTGAAACAACACTCTCTGTTAGCTGTTCGGATAAGATAGCTCGATGGAATGTTCTTGGAGTTCA AGGGGCATTGCTTTACCAAGTCCTCCAGCCTGTGTATATTTCTACCATCACCGTTGGACAATCTCTGCACAGTCCTGACA ATTTTTCTTTAGCTGATCACTTGCGAAGATCTTTGTATGAGAGGATCCTCCCATTATCGGATGAACTTCTGACTTCCTTC CGTTTGAACAAGCCACTGTTTTTTGTAGCTCCAGTACCACCATCAGAGTTTCAGCATTCAGAAACTGCGCAAGCTACTTT AACATGCGGATATTCCTTGTGTTGGAACTATTCTGGCTTACACGAAGTTATTCTTGGAACAACCGGTAGAAAGCAAGGCA CATCAGCTAAAGGAGCTCTGTATCCGTCTACTCAATCATCTATATGCAAGTAAGGGGATGAGCCTTGTTATCCAAGCATG ACCTTCATTATTTGTAGTTGATATCATATTCATATATTTTTCTAGACAGCGGTTACTGGAGCTATTCTTGAA .........................N...................................................... .............................N.................................N...N............ ......................i..................................................N...... ................................................................................ ................................................................................ ..N..........................................................i.................. ......i......................................................................... ...........................................................N.......N............ ...........N................................N................................... ...............N...................................N...................i........ ...........................................................N....N............... ................................................................................ ....................................N.......................N................... ................................................................................ ...i............................................................................ ...........................................N.............N...................N.. ........................................................................ Pos Score Pred ------------------------ 26 0.395 - 110 0.426 - 144 0.369 - 148 0.396 - 183 0.630 Yes 234 0.328 - 403 0.193 - 462 0.528 Yes 487 0.553 Yes 620 0.190 - 628 0.280 - 652 0.144 - 685 0.403 - 736 0.151 - 772 0.331 - 792 0.643 Yes 860 0.260 - 865 0.177 - 997 0.115 - 1021 0.189 - 1124 0.592 Yes 1244 0.173 - 1258 0.051 - 1278 0.187 -

Explain the output -- Processing locus 545: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAGGTATAGAGGTAGAATTAAAGACGAAAACACCTTGGCTACATAAATAGAGCTGATCATGAGCTAACAGGATTGGAT AGTTAATAGTTAGAGACTTAGAGTGGGTATCAAAGTAGAAATCAGAAACTGTGGGGCGAAATCAGAGAAATGTGGGGAGA AATGAAGAGTGTAGTTGCAATGGTGGCGGTTCAGTTTATCTTCGCCGGGATGTTCATTTTGTTTAAGATAACGGTCGACG ATGGTACGAATCTCAAAGTTCTTGTAGCGTATCGTCTGTCCTTTGCTACTATTTTCATGCTTCCCCTTGCCCTCATCTTC CAAAGGAAGAAGCGGCCAGAATTTACATGGAGGCTGCTCTTACTAGCATTTGTTTCGGGGTTGCTCGGAGCGGCGATACC AAATATTCTCTACTTGCCGGGTATGGCTCGAACATCAGCGACATTTTCAGCTGCAAGCAGCATAATTAGCCCATTGATCA CCTTGGTGTTGGGTTTGGTGTTTAGGATGGAGACTCTACGGCTGGGATCGAACGAAGGGAGGGCCAAGCTAGTGGGGACG CTGCTTGGTGCATGTGGAGCCCTCGTGTTTGTATTCTACAAAGGTATTGAGATTCATATCTGGTCAACACACGTTGACTT ACTCAAAGGCTCGCATACTGGTCGAGCCACTACGAACCACCACGTCTCAATCTTAGGGGTTCTTATGGTTCTTGGCAGTA ACGTTTCCACCTCACTTTGGTTACTATTGCAGGCAAAAATAGGCAAGGAACTTGGAGGACTTTATTGGAACACAAGTTTG ATGAATGGGGTGGGAAGTTTGGTGTGCGTGATTATTGCTCTCTGTTCGGATCATGACTGGGAGCAATGGCAATTAGGATG GGATATTAACCTCTTAGCTACGCTTTATTCGGGAATCGTGGTTTCGGGAATGGTCGTGCCTCTTGTTGCATGGTGCATCG CAACAAAAGGACCATTATTTGTTACTGTCTTTAGCCCTATAAGGCTGGTGATCGTAGCCCTAATAGGATCATTTGCTTTA GAGGAACCCCTGCATTTGGGAAGTATAATCGGTGCCATGATAATGGTGGGAGGAGTATACCTAGTGGTATGGTGTAAAAT GAAGGAAAAAAAGAGTGCGTCTACGACTTCGGACCATATTGAAACAAATAAGAATAACAAAGAACTGGACCTTGGCAATC TTTCATCAGTAAATAGAGATGTCCCCTGAATCCCCATATGGGTTACATGATCAAATATAGTTTGAGTCTACCATAGAAGC AGAATATAAAATAGGGTCGGTAAGTGAATTAAGATCCAGCTGTATGCC ............................................................i................... .....................................................................i.......... N.......................................................N....................... ..........................i..................................................... ......................N......................................................... ..........................N..................................................... ...........N.................................................................... ................................................................N............... ................................................................................ i...N...............................................N............i...........N.. .................................................N...................i.......... ................................................................................ ....................................N.....i.........................N.........i. ................................................................................ ..................N..................N........N................................. ...........................................N.... Pos Score Pred ------------------------ 61 0.629 Yes 150 0.681 Yes 162 0.675 Yes 180 0.596 Yes 210 0.120 - 241 0.236 - 297 0.194 - 347 0.870 Yes 423 0.444 - 507 0.323 - 572 0.254 - 705 0.474 - 801 0.517 Yes 805 0.402 - 853 0.145 - 866 0.745 Yes 878 0.434 - 930 0.416 - 950 0.569 Yes 1077 0.240 - 1083 0.724 Yes 1109 0.283 - 1119 0.574 Yes 1219 0.418 - 1238 0.124 - 1247 0.068 - 1324 0.035 -

Explain the output -- Processing locus 546: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGAAATATTCAAATTTCAGTTATTAGATTAGTCGATTAATTTATAACTGATAATCATATTGAGTTTGATACATCCAAAG TGGCATGGGCCTACAGATTAGATGTTTAGGCCAGGTGGACTCTGTTTCGAGCCCATTACTGCCACGTGGCTCTATTCGGA GTTATTTACCAATGGTACGCTCACCGGTTTAACGATTCACCACTTAAAGCCCTCAAAATCTCAGAGAACGCTCGCTGATC AAATCCGTCGTCCTTCAACCGTCGCCTCCTCCGTCATGACAATTGAGTGCGTTAATCCCGAAGGTCTTCGATTCAACCAA ATGAAAAGCTCCTCTCATCAAACCCTAGAAACGAGGATGAGGCCGATTTTGATGAAGGGACATGAACGTCCATTGACGTT CCTGAGGTACAACAGAAATGGTGATCTGCTTTTCTCCTGCGCCAAAGACCACACTCCCACAGTCTGGTTTGCCGATAACG GCGAGCGTCTTGGCACTTACCGTGGCCACAGTGGTGCTGTTTGGTGCTGTGATATCTCCAGAGACTCGTCTAGATTGATC ACTGGTAGTGCTGATCAGACTGCAAAGCTGTGGGATGTGAAATCTGGCAAAGAATTGTTCACTTTCAAGTTTGGTGCCCC TGCAAGGTCTGTGGATTTCTCTGTTGGTGATCATCTTGCAGTGATTACCACTGATCACTTCGTGGGAACTTCCTCTGCTA TTCATGTCAAACGCATTGCAGAAGATCCCGAAGACCAGGTTGGTGATTCTGTGCTTGTCCTTCAAAGTCCTGATGGAAAG AAGAAGATCAATAGAGCTGTTTGGGGTCCCCTGAACCAAACCATTGTTAGTGGTGGTGAAGATGCTGCTATCAGAATCTG GGATGCAGAGACTGGAAAATTGCTTAAGCAATCAGATGAGGAAGTGGGTCACAAGGAGGCCATTACATCCCTCTGCAAAG CAGCTGATGACTCTCACTTCCTTACAGGTTCACATGACAAAACTGCAAAGCTTTGGGACATGAGAACGCTGACTCTTATT AAGACTTACACCACTGTGGTGCCTGTAAATGCTGTCGCCATGTCTCCACTTCTCAACCATGTTGTGCTTGGAGGTGGTCA AGATGCATCAGCTGTGACTACCACTGATCATCGTGCTGGGAAGTTTGAAGCTAAGTTTTACGACACGATTCTGCAAGAGG AAATTGGTGGCGTGAAAGGTCATTTTGGACCTATTAATGCTTTGGCATTCAGTCCTGATGGGAAGAGTTTCTCTAGTGGA GGTGAAGACGGCTACGTGAGACTGCATCATTTTGACTCCAATTACTTCAACATCAAGATTTAGATTCTTTGAACATGTCT TTCCATTTTTTCATCATTCCACATAATATTTTCTCTCTTTTTAACATTTTCAGAATTGATGTACTACCAAATTACCAATA TA ................................................................................ ....N................N.......................................................... ...........i.................................................................... ...................................N............................................ N...................................i..............i.........i.................. .................i.............................................................. ................................................................................ ..................................N............................................. ................................................................................ ...N....................................................................N....... .............................................................N.................. ..N................................N............................................ ......N..........................N.........................i.................... ............................N..........i..................N..................... ..N............................................................................. ....................................N....................N...................... ..........................................................................N..... ..........................................................N..................... .. Pos Score Pred ------------------------ 85 0.298 - 102 0.343 - 172 0.620 Yes 276 0.281 - 321 0.347 - 357 0.600 Yes 372 0.636 Yes 382 0.734 Yes 418 0.738 Yes 595 0.246 - 724 0.123 - 793 0.237 - 862 0.374 - 883 0.155 - 916 0.227 - 967 0.153 - 994 0.097 - 1020 0.828 Yes 1069 0.313 - 1080 0.658 Yes 1099 0.261 - 1123 0.157 - 1237 0.225 - 1258 0.207 - 1355 0.326 - 1419 0.047 -

Explain the output -- Processing locus 547: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGTTAGTTTCTTTGTTGATTATTGATTGCTGAGTAGTTCTAAGTTGATTATGTCAACTCTGAATCATGTACAAGTCTAT GAGCTAGAAAATGAATTGTGAATTGTGGAGCTAAGAATGATGTTGTTGTTTTGGCTGAAAGCGATGGGTACATACCTAAG TTCTCCGAAAACTGAAAAGTTATCAGAAGATGGTGAGAATGATAAGCTCAGATTTGGTTTATCGTCTATGCAAGGTTGGC GCGCTACCATGGAAGACGCGCATGCTGCAATTCTTGATCTTGATGATAAGACATCGTTCTTCGGTGTGTATGATGGCCAT GGAGGTAAAGTCGTTGCAAAGTTCTGTGCCAAGTATCTACACCAGCAGGTTATCAGTAATGAAGCGTATAAAACTGGAGA CGTCGAAACATCTCTTCGAAGAGCATTCTTTAGAATGGATGACATGATGCAAGGACAAAGAGGATGGCGAGAGTTAGCTG TACTTGGCGACAAGATGAACAAATTTAGCGGCATGATTGAAGGATTTATATGGTCACCAAGAAGCGGTGACACCAATAAC CAACCCGATAGTTGGCCTCTTGAAGATGGTCCTCATTCTGATTTCACGGGACCTACCTCGGGGTGCACAGCGTGTGTAGC TCTTATTAAAGATAAGAAGCTCTTTGTTGCAAATGCCGGTGACTCACGTTGTGTGATATCAAGAAAGAGTCAGGCTTACA ATCTTTCTAAAGATCACAAGCCTGATCTTGAAGTTGAAAAAGAAAGGATATTGAAAGCTGGTGGCTTTATTCACGCTGGG AGAATCAATGGAAGCTTGAATCTGACAAGAGCCATTGGTGATATGGAGTTCAAGCAGAATAAGTTTTTACCATCTGAAAA GCAAATGGTTACTGCTGATCCAGATATAAACACTATTGACCTATGTGATGATGATGACTTTCTTGTTGTTGCTTGCGATG GAATATGGGATTGTATGTCAAGCCAGGAACTAGTTGATTTTATCCATGAACAGTTAAAATCTGAAACAAAACTTTCAACA GTATGTGAAAAGGTTGTTGATAGATGTTTGGCTCCAGATACAGCGACTGGTGAAGGTTGTGATAATATGACCATCATCTT GGTTCAGTTCAAGAAGCCTAACCCATCTGAGACTGAACCAGAAGATTCCAAACCAGAACCAAGCGAAGATGAGCCGAGCT CATCAAGCTAGATATCTTTAAAGAGCCAAGAGGAAGAAGATTTGTGAAAGTGTCTCTTTTATCATTGACTTTTTGTTCAA CATTGTAAATCCTTTTGAATGTGGCTTAGA ..................................................N...............N...........N. ..........N.........................N..N.......................i................ .............................N........N............................i............ ........i............N....................N..........................N..N.....N. ..........................................................N..................... ..................................i...N....N..N................i................ ..............i.................N................N.............................. .........................N...................................................... ................................N............................................... ................................................................................ .......N..................................N..................................... ....i.....................................N....N..N..N.......................N.. ....N.........i..............................N.................................. ..N....................N..........................................i............. ....................................................................N........... ................................................................................ ..................N........... Pos Score Pred ------------------------ 51 0.372 - 67 0.172 - 79 0.178 - 91 0.253 - 117 0.476 - 120 0.255 - 144 0.615 Yes 190 0.352 - 199 0.174 - 228 0.772 Yes 249 0.756 Yes 262 0.139 - 283 0.147 - 310 0.227 - 313 0.178 - 319 0.357 - 379 0.420 - 435 0.650 Yes 439 0.230 - 444 0.291 - 447 0.388 - 464 0.721 Yes 495 0.510 Yes 513 0.491 - 530 0.186 - 586 0.334 - 673 0.289 - 808 0.190 - 843 0.367 - 885 0.782 Yes 923 0.243 - 928 0.264 - 931 0.223 - 934 0.266 - 958 0.080 - 965 0.266 - 975 0.755 Yes 1006 0.199 - 1043 0.240 - 1064 0.488 - 1107 0.587 Yes 1189 0.272 - 1299 0.060 -

Explain the output -- Processing locus 548: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGGCGCGCGCTTCATCCCCACCACCTTCGAATTCGTGTACGATCTCTAAAATCCAGTTTTGCTATCGACTTATCTCTTA TTGCGCCATTGTTATGCATAAGAAGGGGATTTTAACAATCTAGGGCCGGTGAATAAGATCATACTTGAAAAATCTGATTT TGTAGATGAAATCGTTGTGGGCAATTTGCTTGTTTGGTTAGGTATTGTTTTTGAGCTAGACCCTTTAGATATAATCGCCA TGGAAGCGCTCTGCTTTGTTTGGATCGAGAAATATATTGTAAGAAGAAGAGAATTGCATGTTTTGGCTATATTGTATTAT CTGATGTTGGTTTGTAATATGGTAGGCCCTTTGAGGGAAGCTGCTAGGATGGGTGTGAAGCAGGCTCTAAGGAGCATTGA TGCGTTTCCAAGAGCAGAGGATCATTTGCTTCAGAAGACACAATCTGGCGCTGTTGTATCTATTGTTGGACTTCTTATAA TGGCTACTTTGTTCTTACACGAGCTGAGTTATTATCTTAACACACTTACAGTGCACCAGATGTCAGTGGACTTAAAGCGT GGAGAAACTCTTCCAATTCATGTAAATATGACGTTTCCATCTTTACCTTGTGATGTACTGAGCGTAGATGCTATCGACAT GTCAGGGAAGCATGAAGTGGATCTTGATACAAACATTTGGAAGCTCCGTCTCAACAGTCATGGTCATATCATAGGCACAG AATATATATCTGATCTTGTTGAAAAGGGGCATGAGCATGGCCACTCACCTCACAAGCATGATGGCAAAGAAGAACACAAG AATGAGACTGAAACCGAAGCATTGAATATTCTTGGCTTTGACCAAGCTGCTGAGACTATGATTAAAAAAGTGAAGCAAGC ATTGGCAGATGGAGAAGGATGTCGGGTTTATGGTGTCCTAGATGTACAACGGGTTGCCGGAAACTTCCATATTTCAGTTC ATGGGCTGAACATCTATGTTGCTCAGATGATATTTGGCGGGTCCAAAAACGTAAACGTGAGCCATATGATTCACGATCTA TCATTTGGGCCGAAATACCCCGGAATTCACAACCCACTTGATGACACCAACCGAATACTCCATGACACCAGTGGAACATT CAAATACTATATTAAGATTGTTCCCACAGAATATAGATATCTTTCAAAAGACGTACTGTCAACAAATCAGTACTCTGTAA CTGAATATTTCACGCCAATGACTGAATTCGACCGTACATGGCCAGCTGTTTACTTCTTATATGATCTTTCACCTATCACT GTGACCATCAAGGAAGAACGCCGTAGTTTTCTCCACTTAATCACTCGTCTTTGCGCTGTATTAGGCGGTACATTTGCTTT GACAGGAATGTTAGATCGTTGGATGTTCCGCTTTATTGAATCTTTCAACAAGAAGCCGAGTACTAGAGCGTAAACTAGCT GAAACAGAGACAAAACAATGAGAAACAAAAAAAACCAAAGGAAGGAAGAAGAATCTCTCAAATCCCAAAACTTGTATCTG CCTTCTTCAGAC ................................................................................ .............N.................................................................. .....N.........................................................................N .........................................................N...................... ...i..............i.............................i..............................N ...............................................................................N ...........................................................i.................... ...................N.......i........................N..............N..........i. ...........N...............................................N.................... ..............................N.....N....................N..N................... ........N.........N..........N...........N...................................... N..............N..........i......................................N.............. ........................................N....................N.................. ................................................................................ .................N...................N......................N................... ................................................................................ .......N..............i......................................................... .................N.............................................................. ............ Pos Score Pred ------------------------ 94 0.117 - 166 0.267 - 240 0.246 - 298 0.229 - 324 0.555 Yes 339 0.754 Yes 369 0.750 Yes 400 0.108 - 480 0.321 - 540 0.652 Yes 580 0.118 - 588 0.667 Yes 613 0.273 - 628 0.176 - 639 0.541 Yes 652 0.216 - 700 0.376 - 751 0.354 - 757 0.395 - 778 0.197 - 781 0.270 - 802 0.240 - 858 0.530 Yes 889 0.195 - 899 0.466 - 910 0.153 - 922 0.187 - 961 0.088 - 976 0.381 - 987 0.678 Yes 1026 0.433 - 1081 0.326 - 1102 0.324 - 1218 0.323 - 1238 0.386 - 1261 0.224 - 1368 0.330 - 1383 0.532 Yes 1458 0.177 -

Explain the output -- Processing locus 549: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGTGTCCCGGCTACGCGCTTTCGTTGCTTCACCTGGAATATTATGTGGCCAATTTGGTTTGGAAATTCGAGTGGAAATG TGTGGAAGGTGAAGAAGTTGATCTATCCGAGAAGCAACAGTTCATCACAATGGTCATGAAAAACCCTTTCAAAGCAAATA TTTATCCAAGGAGAAAGTGATATTTGCTTTTGTCATATTATCAAAAAGGCCCATTGGGCTTATTTGTCGTATGTATATTA AAAACTTTGTCTCATATGTATTAAAACAATTTGGGCCTTTAATGGGTTTCAATATAAAGATGTCAACACTTTTAGTTTTC CTTTCCATCTTTCATGGCGGAGAAGTAGAACAATTGCAAACAGAAAGAAACCCTAAAATTTCGAAAATGGAGGAATCGAA GCAGCAGCAGTTGCAACAACAACAACTCCTACTTCAAATGCAGCAACAACAACAAATGCAACAGCGTCAACAACAGCTCT TTCTGATGCAGCATTTGCAGAAACAGCAGCAACAACAGGCGGCGATGTCGATGTTTCCGCCGAACGCTGACGCTCATCTC CGGCCACCGGGGTTGATTCCGAACCGACCCGTTAACCCGTTTCTTCAGAACGTGAACCCTAACCCTAATTTGATTCAACA AGCCAATAAATTTCAGCAACAGCAGCAGCAACAGATGATGATGATGATGCAACAACAACAACTACAACAACAACAACAGC AGAAATTGATGCGTCCATCGAATCAATTGGAGATACAATTCGCTTACCAAGACGCTTGGCGTGTTTGTCATCCTGATTTC AAACGACCTTTCGCTTCTCTCGAAGACGCTTGTGAAAGATTGTTACCTTATCATGTTGTAGCAGATTACGAAGCAGAAGA AGATGATAGTATCTTTGATTCAAACACAACAAGCCAGACGCTACCCCGCTGTCAGCAATGGGACAACAACATTGCAGCCA AAGTTGCAGAATTCACGGCGACATTTGAGGGACAAGTCCAAGCTTTCGACAGGATAATTCAAAAGAGAAGCGATGGAGAC AGAGTTGAGGAAAGGCTGATGATGGAACAAGTTTTGCTCAATGACGAGAGAAACGCATGTATCCAGCTGGATAGGGAGAT GAAAGCTCAGGATGCGAGGTTAAGAATGGCGGCTTTGGCTCAAGCAGCAGGACAAGCAAGGGCAGAAGAGTCTCAACAGT CTCATGCCGAGATGATGGCTCGTAACCCGTTGAGAGCTAATGCGATTGGGAATCATGGAGAACAGGGGCGTAACATGAAC CCTAATGAGATGATGTTGCTGATGAATGGATGGGGGAACAATAACAACAACAATAATAGCCAGAAGGAAGAGAAGGAACC ATTAGAAGATTTCTTGAATGATGAAGAAAATGAAAATGGTGAACATGAGAAGTGGCGAAGAAGTGGGGATTTTGATTTGA ATATTCGGTAAATGAAACCATGACTCAGATTTTCTTTTGGTTTGGTTATCCAAATACTCGTAGTTTGGTGATGTAGTTAA ATCTTGAAAGGTAGTGAATAAGGTGAAAGA N..........................................N.................................i.. .................................................N.....N........................ ......................................................................N......... ...............N.........................N.................i.................... .............N....................................................N............. .....................................N.................i........................ .....N......................................N.....N............................. ................................................................................ ..................................N..N..N..N..N................................. ........N....................................................................... ....................................................N........................... ..N......................................................N...................... ........................................................................i....... ..................i..i..................N...............i.....................i. ...........N.............i...................................................... ...N.......N..i........................N..............N...................i..... ....N....i..N........i...N...N.................................................. .................N..N........N.....N........N................................... ...........N.......N..................................................N......... .............................. Pos Score Pred ------------------------ 1 0.417 - 44 0.230 - 78 0.640 Yes 130 0.230 - 136 0.133 - 231 0.086 - 256 0.310 - 282 0.466 - 300 0.736 Yes 334 0.493 - 387 0.444 - 438 0.190 - 456 0.543 Yes 486 0.309 - 525 0.477 - 531 0.455 - 675 0.357 - 678 0.194 - 681 0.234 - 684 0.239 - 687 0.174 - 729 0.414 - 853 0.443 - 883 0.205 - 938 0.401 - 1033 0.533 Yes 1059 0.517 Yes 1062 0.749 Yes 1081 0.429 - 1097 0.530 Yes 1119 0.506 Yes 1132 0.317 - 1146 0.776 Yes 1204 0.094 - 1212 0.362 - 1215 0.573 Yes 1240 0.161 - 1255 0.254 - 1275 0.500 Yes 1285 0.157 - 1290 0.769 Yes 1293 0.457 - 1302 0.576 Yes 1306 0.375 - 1310 0.365 - 1378 0.383 - 1381 0.120 - 1390 0.153 - 1396 0.352 - 1405 0.253 - 1452 0.129 - 1460 0.101 - 1511 0.055 -

Explain the output -- Processing locus 550: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGACTAATTCTTTATCAATTTCTTTCTCCAGTAGTGCAAGAAACAAGTTTTCTCTCATCTTTACAGAATTCAAAGCTCT GGTGTAGTTGAAGTCATATAAGACATGGATCAGAATCAGGGTATTACAGTCCTTCCAGGTGATGCCCTTGTGGATTCCAT CAAAGAGAACTTGGAATCTTTGTCTTCTCTTTCAACCAACTGTTGTATCTATAAGGCACCTGAGAGGCTGCGCAGATTGA ACCCCGATGCTTATTCGCCTCGGGTTGTGTCGATTGGACCCTTACACTACGGTAAAGAAGAACTTCAAGCCATGGAAGAT CATAAACTTAGGTACCTACAGAGTTTTATTCCTAGAACAGCCTTAAGTTTGGAAGACCTGGTAAGAGTTGCAAGGACATG GGAAGAAAGGGCTCGCTTTTGTTACACAGAAGATGTAAGACTTAGTAGCGATGAATATGTGAAGATGTTAATCGTTGATG CAAGCTTCCTAGTAGAACTTCTTTTGAGGTCTCAGTTTGATGTATATAGAGGTATGCTAGATAGGATATATGGGAAGCAA AAGATGATTGTCGATGTGAACCATGACGTTATGTTACTTGAAAATCAGCTTCCATATTTTGTTGTCGAGGGGATGTTTGG ATTGCTACATGTTGACTACCATAGGGAACTTCCCCCTCTGACTCGAATCATTCACAACCACTTCAAGAAATTCTGGATGA GCATCCCTTCATTTTCGAGAAGCATTTCTGATTCAAAGATCTGTCATTTTGTTGACCTTCTGCGATCTATTCACCTGCCT TTGGTTTTGAGTTTTCCTGGAGGAAGCATGAGGATGATGGACTCTGTACTGAGTGCAAAAGAGATTCAAAATGCCGGTGT TAAGCTCCAGCCCGCAGATAACAACACCTGCGCACTTGACATAAGCTTTGCAAACGGAGTCCTCACGATTCCCAAAATCA AGATCAACGACATCACAGAATCTCTGTACAGAAACATCATCTTGTTTGAGCAATGTCATCGTTTAGATGCTTACTTCATT CATTATATGAGGTTCCTCAGTTGCTTCATAAGATCTCCCATGGATGCTGAGTTGTTCATTGACCACGGGATCATTGTGAA CAGATTTGGAAATGCAGAAGACGTTTCGAGGCTGTTCAATAGCATATTGAAAGAGACAAGTTACAGCGGGTTTTACTATA AGACAGTCTATGGGAATCTACAAGCGCATTGCAATGCGCCTTGGAACAAGTGGAAAGCAACTCTGAGACGTGATTATTTC CACAATCCTTGGTCAGCTGCTTCTGTTGTGGCTGCCTGTGTACTTCTCCTTCTTACTTTCGTTCAGGCTATATGCTCTAT ATTAGCTTTATGACTTGTGAGACAGTAAAGGATAATCAAATGTTGGGAAAGAGGAATGTTATATATGCAGAGAATCTTGT AACAGAAGCAACATAAGATTACCAGTTATGTG ................................................................................ ........................i....................................N.................. ................................................................................ ......N................................................................i........ .............................................................................N.. ................................N.................N.....N.......N............N.. .......................................N.............N...............N.......... ...i.........N........N.......N.........................................N....... ........N...................................................................N... ................................................................................ ...........................i.....i..i.................................i......... ................................................................................ ....................................................i.............N............. ......N................................i...N.................................... ...........N.................................................................... .........N.......................N.............................................. .......................................................................i........ .........N.............................N...............N........N............... ...........................N.... Pos Score Pred ------------------------ 105 0.582 Yes 142 0.125 - 247 0.208 - 312 0.740 Yes 398 0.446 - 433 0.226 - 451 0.160 - 457 0.205 - 465 0.280 - 478 0.195 - 520 0.141 - 534 0.323 - 550 0.160 - 564 0.535 Yes 574 0.117 - 583 0.389 - 591 0.246 - 633 0.132 - 649 0.272 - 717 0.248 - 828 0.662 Yes 834 0.697 Yes 837 0.596 Yes 871 0.561 Yes 1013 0.694 Yes 1027 0.377 - 1047 0.453 - 1080 0.801 Yes 1084 0.246 - 1132 0.445 - 1210 0.242 - 1234 0.185 - 1352 0.632 Yes 1370 0.484 - 1400 0.353 - 1416 0.104 - 1425 0.041 - 1468 0.042 -

Explain the output -- Processing locus 551: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGTGTCTCCCTTATTTTCAGGCACTTCAAAATAAATATACGAAAATAAAATAACCATTTCTTAAGTCTAAAGACCTTCT CTTTCAACTAGAAGCCTTTAAGCCATGTCTTCTTCTTCCTTGATACTTCTCTTCTCTACTCTCTCTCTCTTTCTCAATGT CTCACTCGCAGACAATCACACGGCGGTTGTTATAACAACCTCAGATACTCCACCGCCACTTCCTCCGCCTTCTCCACCGC CACGGCATAACTTCACTTCCTCTCTCATGCCCGGAATCGCAGTCGTTATCGCCGTCCTCACTGCGTTTTTCTCTCTCACT TTCTTACTTCTCCTCTACGTCAAACACTGTAAACGACGAAACGGCAGCGTTTACGTAAACCACCCGCAGCGTTTCGCAAT CACTAGATACGGAGGAGGATATTACAACGGTGGAGGAGTAGTAGGAGGAAGGAAAAACTCCGGTATAGACCGGTCCGTGA TTGAATCTTTACCGGTTTTCCGGTTTGGTGCATTAAGCGGTCACAAGGATGGTTTAGAGTGCGCCGTGTGTCTCGCTCGG TTCGAACCGACGGAAGTTCTGAGGTTATTGCCGAAATGTAAACACGCCTTCCACGTGGAATGTGTTGACACGTGGCTTGA CGCTCACTCCACTTGCCCGCTTTGCCGTTACCGGGTCGACCCGGAAGATATACTCTTGATCGGTGATTGTAACTCCTGGT TCGAGCTCCAGTTCTCTAAAGATGAATCAAACAGTGTTAATAATAATCCTCCCGGTTTAACCCGGTTTATTCCGGTTAGT CGAATTTCCGGTCGTCATTCCTCGGCTGGTGAACGAGCGAGTCGACTCAACGAGATTAGAACATCTTCGTCGTATAAATC GAATCCGATGTCGTTTCGGAGATCACTCGATAGCTCTCTGAAAGTTAACGACGCCGGCGAGGAGAAATCGGAATCTGTCG CCGTTAATTGCTTGGATCGTCTACAGAGAAAAGACGGATTGTTGTTGATTCCGAATCGGGAGAGCTTTGAGGGGAGATTT GAGCACCGGATAATTATCTCCGGCGGGAATCGAGACGATCAGAGGTGGAGCGAAGTAAGGCCGTCGGATCTTTTGTATTT ACGATCAGAGATGATACTCAGCGATTGCCGGAAATTAGCTGCGGCGGAGGGTGGCAGAGATGTAATTAACGGGAGAAGTG TGTCGGAGTTAACGGGAATTGAGAGACGGAGGAGATGGGGAGGAGAGCCGAGACAACGACAAGCCACGGCGGTGATTTCG AGGTGGCTCGCTTGGTCCCACCGTGCCTCCGCTTCCAGCATTGTTTAAAATTTATATTATGATCCTTTTAGTTTTGTTTT ATAACAGTTTTGGCCGGTTTAGTTTTGTTCTATAACATTTAGCCCAAACTAATTTATTAGAGGAATG ................................................................................ ........................i...................................................N... ................................................................................ ..........................i..................................................... ................................................................................ ................................................................................ ................................................N............................... ...................................N.......................N.................... ................................................................................ .....................i.......................................................... ................................................................................ .......i........................................................................ ................................................................................ ................................................................................ ..........N................................................i.................... ..................................i............................................. ..........................................................N..................... ................................................................N.. Pos Score Pred ------------------------ 105 0.626 Yes 157 0.320 - 267 0.621 Yes 529 0.495 - 596 0.132 - 620 0.265 - 742 0.565 Yes 888 0.701 Yes 1131 0.325 - 1180 0.587 Yes 1235 0.534 Yes 1339 0.119 - 1425 0.041 -

Explain the output -- Processing locus 552: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGATTATATTTTCCTTTTCTCAAACATCGTAACCATACACGTATAGCTTTTTCAACGTTTTTATGTTGATCTATTAGCT AATAATTAACATTTTACGTGAAAAATGAAGGGTTTAAGGAAATTTCAGCAAATGGCCAACGATCAGAAGAATAGTGAGTC TTTTCCAGCGAAGGAAGATCATAAGAAGGATGATGCGGCAGCTCCAGCAGAAGTAGACCATAAGGATGAATTCTCGGCAT CACAGCCACATCCGGTCGAAAACATAGTTTTAGTTGGGCGTACAGGGAACGGCAAAAGCGCCACGGGGAACAGCATCGTC AGATCAAAGGTGTTTAAGTCGAAAACAAAATCATCAGGTGTTACGATGGAATGCCATGCAGTTAAAGCAGTGACACCAGA AGGCCCTATACTCAATGTGATTGACACTCCTGGTCTATTTGATTTGTCGGTATCTGCTGAATTTATCGGTAAAGAAATAG TTAAATGCCTAACTCTAGCGGATGGAGGGCTACACGCTGTGCTCTTAGTTTTATCTGTAAGGACTCGAATTAGTCAAGAG GAAGAGATGGTACTTAGTACCTTGCAGGTTCTTTTCGGTAGTAAGATTGTTGATTACCTTATCGTTGTTTTCACGGGGGG AGATGTATTGGAAGATGACGGTATGACATTGGAGGATTACTTGGGTGATAACATGCCGGACTTCCTAAAGAGAGTTCTCA TATTGTGTGGCCAACGAATGATTCTGTTTGATAACAAAACCAAGGATGATGAGAAAAAGACAAAGCAAGTCCATGAACTT CTCAAACTTATCGACTTGGTCAGAAAGCAGAACAACAATATTCCGTATACCGATGAGATGTACCATATGATAAAGGAAGA AAATGAAAGGCACAAGAAAGAACAAGAGGAGCTAGAATCAAAAGGGCATTCAGAAGAGCAGCTCGCAGCATTGATGAAGG AGTTACAGATAATGAATGAACGAAACCTCAAGGCAATGGCAGAGATGATGGAGAAAAATATGAAAATAGCTATGGAGGCG CAGGAGAAGCTCTTTGAGCAAAGAGAAAAAGCTCAGGAGATGAGCTACCAGCAAAAAATGGAAATGCAGGAGAAGTTAAA GCAGATGGAAGGACGTATGCGTGCTGAAATGGAAGCGCAAATGCTCAGCCGTCAGCAGTGCAGCATTCTCTGATCATTCA CTGTGCGCTCTTCCACCCTCACACTTTGCCTTCTTAATGACAAATAGAGAACCATTGCCTAGACTTTGTGTTTCTAACGC GGAACATTTTCT ...............................................................N................ ........................i..........................i............................ .............................N..N................................N.............. ................................................................................ .............................................i....N....N........................ ..............N................................................................. ....i................N.......................................................... ......i......................................................................... ..N...........N.......i.............................i........................... .................i...........................N..N.......................N....... ....................................................N....i........i............. ..i......................................................................N...... ...........N...N...................i........N..i...........N...........i........ .......................................N.................i.....N................ ....N...........N...........N...........N....................................... ....................................N........................................... ............ Pos Score Pred ------------------------ 64 0.197 - 105 0.892 Yes 132 0.893 Yes 190 0.338 - 193 0.333 - 226 0.440 - 366 0.592 Yes 371 0.323 - 376 0.143 - 415 0.236 - 485 0.776 Yes 502 0.188 - 567 0.591 Yes 643 0.106 - 655 0.301 - 663 0.574 Yes 693 0.675 Yes 738 0.522 Yes 766 0.260 - 769 0.234 - 793 0.270 - 853 0.282 - 858 0.535 Yes 867 0.515 Yes 883 0.607 Yes 954 0.235 - 972 0.465 - 976 0.109 - 996 0.672 Yes 1005 0.462 - 1008 0.579 Yes 1020 0.377 - 1032 0.674 Yes 1080 0.394 - 1098 0.513 Yes 1104 0.236 - 1125 0.369 - 1137 0.273 - 1149 0.346 - 1161 0.316 - 1237 0.077 -

Explain the output -- Processing locus 553: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCCTTGGACAAACAAAAGCTAGACGTCTAAAAATAGTTAGAAAATAAAACATATATAAGATGAACTAAACACTTTGGGGG AAAAAAAAATTATGAACTTAATTAGGGTTCTATATGTTTAGAAAAAAAATGTTGGTGTTAGCATTATTTTCCATTTACTT CTTATCAATAGAAGCTGTCCGGAATGAATCATTTCCTGCGCTATTAGCTTTTGGAGATTCAATGGTCGATACTGGTAACA ACAATTACCTCCTGACTCTAATGAAAGGAAATTACTGGCCATATGGGTGGAATTTCGACTCAAAAATACCGACCGGAAGA TTTGGAAACGGAAGAGTTTTCTCCGATGTAGTTGGTATAATATTAAAGAGTTCTTTACAATGCTTTTTTGTAATTTCTGC TGAAGGTTTGGGGATCAAAAGAATTGTACCAGCTTATCGTAAGTTATACATTGCTCCAAGCGACCTTAAAACGGGTGTTT CTTTTGCATCAGGTGGTGCAGGAGTCGACCCTGTTACATCCAAATTGCTGAGAGTTTTATCGCCAGCTGACCAAGTAAAA GATTTCAAAGGCTACAAAAGAAAGCTAAAAGGTGTAGTAGGCAGGTCAAAAGCAAAAAAAATAGTTGCAAATTCAGTGAT TCTTGTTTCTGAAGGAAATAATGATATTGGAATCACATATGCGATTCATGATGCTGGTATGCGATTAATGACTCCGAAAG TATACACCAGTAAATTAGTTGGTTGGAACAAAAAATTTATAAAAGATTTATATGATCACGGAGCAAGAAAATTTGCGGTG ATGGGAGTGATACCATTGGGATGTTTGCCTATGTCAAGACTTATTTTTGGTGGGTTCTTCGTGTGGTGTAATTTCTTGGC AAACACAATCTCTGAAGATTACAACAAAAAATTGAAAAGCGGAATTAAAAGTTGGAGAGGAGCATCAGATTTTAGGGGTG CTAGGTTTGTCTATGTCGACATGTACAACTCTCTTATGGATGTTATCAACAATCATAGGAAATACGGATTTACCCATGAG AAGAATGGATGTTGTTGTATGCTTACGGCAATAGTACCATGCTCCAACCCAGATAAATATGTCTTCTATGACTTTGCTCA TCCCTCCGAGAAAGCCTACAAAACAATTGCTAAAAAGCTTGTCGAGGATATCAAGACAGGACTTGCCTGATTTCTTACAA TGCTATTTTCATCTTGGAATTACCATAATTAATTATATACGTACTTCTTCTGTTTCAAAATCTTTGATGTTCTAGAGAAT TTTTTTTTG ...........................................................N.................... ...........N.....................N..............N............................... .......................N.....................................i.................. ....................N.....................N..................................... .........................N.................................N.................... ................................................................................ ................................................................................ ................................................................................ ....................N.................N........N..N.......N........i............ ...................................................N............................ N...................N.........N................................................. ................................................................................ ............N.......N..............N...N...................................i.... ....i...i.........i...................N...................i........N............ ...............................................................................N ..................................................................N............. ......... Pos Score Pred ------------------------ 60 0.158 - 92 0.201 - 114 0.107 - 129 0.491 - 184 0.233 - 222 0.635 Yes 261 0.279 - 283 0.469 - 346 0.171 - 380 0.299 - 661 0.349 - 679 0.256 - 688 0.153 - 691 0.160 - 699 0.268 - 708 0.510 Yes 772 0.319 - 801 0.424 - 821 0.212 - 831 0.097 - 973 0.406 - 981 0.345 - 996 0.306 - 1000 0.127 - 1036 0.711 Yes 1045 0.614 Yes 1049 0.518 Yes 1059 0.641 Yes 1079 0.357 - 1099 0.504 Yes 1108 0.095 - 1200 0.099 - 1267 0.042 -

Explain the output -- Processing locus 554: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAATCAAAACGGAGAATATCAACGTTGAGAACTACCCCGTGAAGCACTGATACCTAGCACTACCAAACCCAAAAGGTGT ATCAAAATTCTTGAATATTAAATGGGCTTTTTAAACATATATTATTGGGTTTGCAAGGGTGAAAGGCCCAAATTCGTCGG TAGATACTTAAAACAAACGACACACGTTTCTAACCTCTCAGAGCAAAACGTCATGCAAGTCGAAATCCCATCGCCGTATG ATCACGGAAGCAGAAGGCAACGGCGAATTCGACGAACGGAAGAAACCCAAAAGCAGAAGAAGATGGTGGCGATATCTATG TACAGAGGCAACCTTCACAAAGTTCCCGACGTACCTCGCCGGTGGATAATGCCTGACCGTAATCTCTCTTTCAAGGATTT CAAATCTCTTCTTCATCGCCGTAAAAAAGCCCTTTCTCGTCTCCCCCTTAACCCTAATTTGAACCTTAGCGTCAAGACTG AGTTGGTTACTGATCAGGAGAATCCAATTCTTCCATCTGAAGCGAACGGTTCTTCTGGGAAGCAGAAGCTATTTGAGGTA AAGAGAGAGGAGATTTGTGGGAATCGGGTTAAGGGAGATGAGAACAACGACAGAGGTTTTGAAGGAGCTCGATCAGACGG TGGAGATCGTCCCGGGAGAGTGACGGAATCAAAGGAAACCGATAATGTGCCACACAAGTATGCGGCCAAGGAGGAGGAGA CGAATGAAGCAGCTGAAAAAGTACCAAGCGAGACAGAGTTAAAACGGAAAGAAGTGGAGGAGAGATTACAAGTTTTGAAT GCAAAGAAACACAATCTAGTCCAAGTGCTTAAGCAGATTTTAAATGCTGAGGAAGAATTGAAAAGACGCAGCTACATGCA ACAACAAGGAACCACCGTAGCCACTCGTCCTTCTCTTCCACTCCATGTGGATGTCTCGAATGACTCAGGAGGTAATGTTG GTACTCACATGGAAGGCGGGGAAACTGATGATGCAGCGAATCATAATAATGCTCAAACTCGTACTCTGCTTCGACTGTGT GGCGCTTCTTCATCATCTGAATCTCCTCTGAGAAGAGCAGCAGCCTTGTCACAACACAATATGGTTCCACATACTTCTCG ATGGAGCCCGCTCGTTGGTCCTTCTCAACCCGGTCCTGCAGTCACTGTTTCTGCGTCTGGAACAAACTACATTGCTTCAT CTCCTTCACCAGCGGGTTTTGGTGGCACTTCTGTTTTCAGAGAATCACGGCTTCAGAGTCCATGGAATTAGCCAGCTTCT CCTATATCTTTTTGTTTTTCGTAGCTGTAACGTGTTCATATTGATTGATGTATAAATCAGACTCATCCATATCTTATTAG TATTACTGCA ................................................................................ .....................N.......................................................... ....................................................N........................N.. ..............................................................i..............N.. ................................................N............................... ................................................................................ ................................................................................ .....................................N.......................................... ............................................i..............N.................... ...N..........................................................................N. ...........................................N...............................i.... ............................................N.....N........N..............N..... ........i..................N..i.................N............................... ............................................................i................... N............................................................................... .............................................................N.................. ...............................................N................................ .......... Pos Score Pred ------------------------ 102 0.415 - 213 0.369 - 238 0.303 - 303 0.541 Yes 318 0.223 - 369 0.395 - 598 0.191 - 685 0.551 Yes 700 0.125 - 724 0.168 - 799 0.271 - 844 0.266 - 876 0.576 Yes 925 0.173 - 931 0.144 - 940 0.203 - 955 0.244 - 969 0.651 Yes 988 0.383 - 991 0.571 Yes 1009 0.220 - 1101 0.726 Yes 1121 0.447 - 1262 0.148 - 1328 0.043 -

Explain the output -- Processing locus 555: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTGTGTATAATAATATTCACGGTGGTATAAAGGATACTTGCAAGTGTGAAAAAATCTGAACTTGAGACTTTTGTGGTAA ATGTTTCACGATTCTTTATAGTATATATCACTGGACTGTAAAAACGAAATGCAGTCCTTGATTTTGAAAACAAAACGGCA CGTAGTTTCTTTGTACTGTGAATCAGTTAAAGTACAACAGACCTTGGAGTCTCTCAGTCCTCCGATTTCAATGGCGGTTT TTCAGTTACCAAGTTTGTCACCGGAGATTCCTTTCCGTTTTACTCCCACACCACTTAAATTTTCAAATCGTAGGAGAATC AACTTTTTCCGGCCATTATCAGCAACCGACGATTCTCGATCCGGTGCACCAGATCCAGAATCGCCGAACCAAATGTTCAT TCTCGGGATGGGATTCGTCGGAGGTTTTTTTGCGCAACAGCTCAAAGAATCAGACTGGGTGGTTTCAGGGACTTGCAGGA GTGATTCTAAGAAGAAAGAATGGGAAAAGAGAGGAATTAATCTTCATCCATTTTCTGCAGACTCACCTGAATGGAGCTTG CTAGATTCAGTGAAAGATTACACTCATCTACTCATTTCCATTCCTCCTTTAGCTGACATTGGTGATCCGATGTTGCGGAA TGTAGAACTTGTGAGAGACAAGCTTTCGAGTGGAAATCTTCGATGGCTTTGTTATCTCTCATCAACAAGTGTGTATGGAG ATTGTGGTGGTGCGTGGGTCAATGAAAATCATCTCCCAAATCCTAAAACTCAGTCAGCCAAAGTGAGATTAGCTGCAGAA CAAGGATGGTTAAGCTTGGGTCGTGATCTTGGGGTTTCGACTCAAATACTTCGACTTGGGGGTATTTATGGACCTGGTCG AAGTGCAATTGATACCTTGTTAAAGCAGGAGCGTTTGTCTGAAGGTCAAAAGAGAAGAGCGTCCCGTAAATTCACGTCGA GAGTTCATGTTGAAGATATATGCCAAGTTCTTAAAGCTGCCACTGAAAAGCCAGCATCAGGGGAGATCTACAATATTGTT GATGATGATCCGGCAGCAAGAGAAGAGGTGTTTGAATATGCCTTGGAGTTGATCGAGAAACGTTGGCCGGGGAATATCAC CACAAAACCATTTCCATTTCTGTATGAATCTCGGGAGGAAAGTTCATTGAGAGGCGAGAAACGAGTCTGCAACGAACGCA TGAAGGATAAACTAGGAGTGAAATTGTTATATCCTTCGTATAAGTCAGGACTGCAAAGCATAGTCGAGAACATGGACAAC CGTTTTTGAGCACACCACAGGACTAAATTCCGCTGATCTGAATCTTTCCTGTGGAGAATTAGGGACTCTTTAGTAAAGAT AGATTTTTAGTTTATCCTTTTTTGTTTC ................................................................................ N...............................................N............................... ......................................................................N......... ................................................................................ ........................................................................i....... .......i........................................................................ ...................i..................................................N......... .....................................................................N.........i ..........................................N...............................i..... .....................N.......................................................... .....N.............................................................N............ ................................................................................ ......N............N............................................................ .......................N.......................................................N .......................................................................N........ ................................................................................ ............................ Pos Score Pred ------------------------ 81 0.146 - 129 0.391 - 231 0.138 - 393 0.536 Yes 408 0.664 Yes 500 0.666 Yes 551 0.266 - 630 0.389 - 640 0.676 Yes 683 0.429 - 715 0.574 Yes 742 0.185 - 806 0.319 - 868 0.359 - 967 0.320 - 980 0.199 - 1042 0.085 - 1045 0.156 - 1078 0.437 - 1144 0.184 - 1200 0.443 - 1272 0.231 -

Explain the output -- Processing locus 556: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTCTTTGTAAGCTAATATGGTATTGTCTTAGATCAGAGCAATGCACATGTGATGTTATATCTGAGATCATGGAATTTAA AGAAGAAACTTTGTATCATGAGTAATGTTGATATGTTTAGTGATGAAAAGTTCTCAGATGTCAGAAACAGAATTTAAAGT GTTGCTTGAGACATTCACATTTCCTGCTGATTCTGCTCCCTTCAAGAGTTGTCATGCCTCTACCATAGTTGAGGTTGTTA AAGATCATTTCTTGGCTGCGTATTTCGGAGGCACCAGGGAAGGAGCACCTGATGTCAAAATCTGGTTGCAGCATTTCAAG GATGGTCAATGGGATTCACCGGTTATTGTTGATGAAGAGCCAGGAGTTCCAATGTATAACCCTGTCTTGTTCAAACTTCC ATCTCATGAGCTCTTATTGTTCTACAAAATCGGCCAGGAGGTTCAAAAATGGAGTGGTTGCATGAAACGATCATATGATA AAGGTATAACTTGGACTGAAAGAGAACAGCTTCCTCCTGGGATTCTCGGTCCTATCAAAAACAAGCCCATTTTGCTTGAA GACGGTACTCTCCTTTGCGGATCATCTGTTGAGAGTTGGAATTCTTGGGGAGCATGGATGGAGGTCACTTCAGATGCGGG TAGAACATGGAGAAAGAAAGGGCCAATATACATTCAAGGCAAGAGTCTTAGCGTGATCCAACCAGTTCCATACCAAACCG CAGCTGGGAACTTACGGATTTTACTCAGATCCTTTACTGGCATTGACAGAATCTGTATCTCAGAGTCTTTGGATGGCGGT GAGAACTGGAGTTTTGCAGTACCAACGGTTCTCCCAAACCCAAATTCAGGTATTGATGGAGTCAAGCTTAAGGATGGTAG GCTAGTGCTTGCTTACAACACAGACTCAAGAGGTGTGCTCAAATTAGGAGTTTCTTTAGACGACGGTGATTCATGGACCG ATATTTTAACGTTGGAGGAATCTCCGGGAATGGAGTATTCGTACCCGGCTGTTATACAAGCCGGAGACGGCAATGTCCAC GTCACCTATACATATAACAGGACACAAATTAAGCATGTGGTGCTCAAGACTGCGACAGATATCGATTCGAAGCCATACCG ACATGGTGAAGCAGGAGGAGGACACGTGAACTTGGATTTGTACGAACCAAACATTAATTAATTAGCTATTTGTCTCTTGA ACCATCGATGTGGAGTGTGACTTCCTTCTTACGGAAGAACAAAGAATGTTCTCTTTCTTGTCTTGTAAGTTATTGATTGA .................N.......................N.....N....N................N.......... .................N......N.......N.........N..............i...................... .....................................................N.......................... ...................................................N............................ .....N..........................................i............i............N..... ................................................................................ .....................................................i...i...............N...... ......i......................................................................... ........................................................................N....... .......................................................N.................N...... ........................................................................N....... .............................N..........................................N....... ..................................N............................................. ..N............................................................................. .......N.....................................N.................................. Pos Score Pred ------------------------ 18 0.204 - 42 0.291 - 48 0.202 - 53 0.145 - 70 0.190 - 98 0.287 - 105 0.441 - 113 0.311 - 123 0.390 - 138 0.827 Yes 214 0.220 - 292 0.210 - 322 0.160 - 329 0.311 - 352 0.137 - 372 0.363 - 406 0.335 - 449 0.532 Yes 462 0.502 Yes 475 0.205 - 614 0.519 Yes 618 0.600 Yes 634 0.325 - 647 0.714 Yes 793 0.345 - 856 0.459 - 874 0.384 - 953 0.333 - 990 0.465 - 1033 0.336 - 1075 0.492 - 1123 0.219 - 1208 0.060 - 1246 0.059 -

Explain the output -- Processing locus 557: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGAAGAGAGGCGGAGAAGCAGAGATGACATAGCTAATAGCTTCGCTTGGTTGCTCTGTGTGTTTCTTCTCTAAAGGGTT TTCGAGTTAGTCAAGATCTAATTAAGACCAAAGTGCTTGCCACAAGAAAAAGGAGAAGTAGGGTTCTCAATTATTACTAG AGAGATGTCTCTTTCTCTTTATTTTTTCCACGTTTCTCTCGTTTTTCTCCTTCTTATGTGTCTACTTCTTACTCTTCCCC AGAGATGCCGCCACAGTACCCTTCGTATGTTCTCCTCCTCCTCCACCACCACCAACCCTTACTTGATGTATTATGTCACC TATTCTGATGAAGCCCCACAGGCCGGTGCAATGACAGAGCTCCGCATGTTTGACCCGGCCAAGGAAGAGTATTTCACCGT CCATGATAAGCCACTTCCCAAAGAGTTGGTTAAGTCGAGGCTGGTGGGAAGTTCCCATGGTTGGGGAGTTTTTCTTGGCT CCCCTAATTCCATACTAATTAGCGACTACTGCAATCCCTCCAGCTCCAAATCAAACCCCAAGATGATCCCTCTGCCTCCA CGGAGTTATGAACACTCTTGTCAAAATGAATTGGTTAGTGGCGTGGCAATGTCCTCTTCTCCTGAAGAAGAAGACTTCGT TATGGCTGTCAAGTTCACGGGACGTCGGGTGAGTATATACAGGCCCGGTCAACATCGTAAGGGGAACCACCGCTTTTCGT CTACTGAACTGTTCGATTATTTTGAACAAGCCAACTTGATGTATTCCAAGAGAGATCAACGGTTCTACATGCCAAGTTCT GGAGGCCACCACTTGTGGTCTTGGGATGGTATAATATCCACCGACTCTCAGTACCATGACTTACGCTTTCACAACCTTCC TCAGTTTTCACTTTCCGAGTTGCAGCTTTTGAGATCTTGTTACAGAACACAACATCTTGTGGAGTCTCCCTCAGGACAAC GTTTCCTAGTCAAATGGTCTGTTCAAACCCTTGGCAGGTCTGTTCAAAGCCTTGCGCATCGGTCGGTAGAATTCAGTTTT GGCGGCACAAAGCTGTTCATGGTGTTTAGAGAGGAGGAGGATATGAACATGTGTTACACACAAGACATTGGTGATCTCTG CATATTCCTCGGCAATAACGAGCCTTTTTGTGTCAAGGCCAGTTCATTCCCTGGCCTAAACCCTAATTCCATCTATTTCA TAGGTGACCGTTATGGTGAAGGCTATGGCGTTTACGATATCGCCACTAGAACCCCACGTTCCTTCAATCCCAAGTCTTTT TCAGCTTTCTCTGATTTACCTAATGGCCAAGGTTACGTTCTTCCTAATTGGCTTCCTCACTGGATTCCTCCATTTCCTCT CTAGTTGTTAGGTCATTCGTTTTCAAAAGCTTCATTATGCTTATTTTTCTCTTTTCCTTTTAGCTATAAATCGCAATTTG CATTATCATCTTGCTACTCTGCT ........................i....................................................... ................................................................................ ....N..................................................N........................ ....i.....................i......................................i......N....... .......N......................i..............i.................................. ..N.....................................................i....................... ..............................................................i................. .......N.................i......................i............................... ......................................N.............................N........... .........................N.............................N........................ ................................................................................ .............N.................................................................. ..................i.......................N.....i............................... ................................................................................ ............N...........N....................................................... ......................N......................................................... ....................................N........................................... ....................... Pos Score Pred ------------------------ 25 0.739 Yes 165 0.390 - 216 0.310 - 245 0.636 Yes 267 0.821 Yes 306 0.660 Yes 313 0.353 - 328 0.248 - 351 0.842 Yes 366 0.545 Yes 403 0.235 - 457 0.781 Yes 543 0.654 Yes 568 0.458 - 586 0.506 Yes 609 0.792 Yes 642 0.727 Yes 759 0.469 - 789 0.249 - 826 0.294 - 856 0.176 - 974 0.308 - 1059 0.668 Yes 1083 0.245 - 1089 0.600 Yes 1213 0.306 - 1225 0.398 - 1303 0.275 - 1397 0.058 -

Explain the output -- Processing locus 558: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTCAAATACTCTTCCTAATCTCTTCTGTTTTCATTTTGCTTGTTCCCTGCGAACATCTCTCTCTCTTCCTCTGTTTCTT GTTTTTTTGTCCTTTTCTTGATTTTTGTATTTTTTTGTTATGGATATGGACGAGATGAGCAATGTAGCCAAGACAACGAC AGAGACTTCAGGCTTAACTGACTCTGTCTTGAGCCTCACGAAACGCATGAAACCTACTGAGGTTACGACCACCACAAAAC CTGCCTTGTCCAACACGACGAAATTCAAAGGAGTTGTTCAGCAACAGAACGGTCATTGGGGTGCTCAGATTTACGCAGAC CATCGAAGGATTTGGCTTGGAACTTTCAAATCCGCTCATGAAGCCGCTGCTGCTTACGATAGCGCATCGATTAAGCTTCG AAGCTTTGATGCTAACTCGCACCGGAACTTCCCTTGGTCTGATTTTACCCTCCATGAACCGGACTTTCAAGAGTGCTACA CGACAGAAGCTGTGTTGAACATGATCAGAGACGGTTCTTATCAACACAAGTTCAGAGATTTTCTCAGAATCCGGTCTCAG ATTGTTGCGAATATCAACATCGTGGGATCAAAACAAGTCTTAGGAGGAGGAGAAGGTGGTCAAGAATCGAACAAGTGTTT CTCGTGCACGCAGCTTTTTCAGAAAGAACTGACACCGAGCGATGTAGGGAAACTGAATAGGCTTGTGATACCTAAGAAGT ATGCAGTGAAGTATATGCCTTTCATAAGCGATGATCAAAGCGAGAAAGAGACGAGTGAAGGAGTAGAAGATGTGGAGGTT GTCTTTTACGACAGAGCAATGAGACAATGGAAGTTTAGGTATTGTTACTGGAGAAGTAGCCAGAGCTTTGTCTTCACCAG AGGATGGAATGGTTTCGTGAAGGAGAAGAATCTCAAGGAGAAAGATATTATTGTCTTTTACACTTGCGATGTCCCCAACA ATGTGAAGACATTAGAAGGCCAAAGCAAGACCTTCTTGATGATTGATGTTCATCACTTTTCAGGCAACGGTTTCGTGGTT CCCGAGGAAGTAAACAAGACGGTTCATGAGATTTCTGATGAAGAGATGAAAACAGAAACCCTCTTTACCTCGAAGGTAGA AGAAGAAACCAAATCAGAGGAGAAGAAAGGAGGGTTTATGCTGTTTGGTGTTAGGATCCAATAGCTAGCTACTTGGTTCT TAGGTTTTTTATATTATAACTTAATTAGAGTATGGTTTTTATTTGCTTTTGTATTACATAGTTTTTTTATTTACAAACTC GAT ................................................................................ .......................................i.....i........i......N.................. ..............................................i................................. ................................................................................ .....................................N.......................................... ........N............................................N.......................... ....................N........................................................... ................................................................................ .........................................i...................................... N.............N...............N......................................i.......... ..................i.......N..................................................... ...N....i...........................................................N........... N.....................................i......N.................................. .........................N...........N.......N.................................. .....................................N.......................................... ...............................N................................................ ... Pos Score Pred ------------------------ 120 0.716 Yes 126 0.791 Yes 135 0.649 Yes 142 0.263 - 207 0.524 Yes 358 0.340 - 409 0.149 - 454 0.346 - 501 0.360 - 682 0.537 Yes 721 0.160 - 735 0.420 - 751 0.146 - 790 0.610 Yes 819 0.582 Yes 827 0.426 - 884 0.163 - 889 0.508 Yes 949 0.190 - 961 0.322 - 999 0.633 Yes 1006 0.107 - 1066 0.108 - 1078 0.219 - 1086 0.469 - 1158 0.142 - 1232 0.070 -

Explain the output -- Processing locus 559: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTACTTCGATTCATGCAGGTTTCTGACATTTCCACTCGACTCAGATTTTGATTTCATCTGAAAGTTTGGATTTTTGAGT TGGAAAAGAAGTGAAATCTGAAGCGATATAGCGACAATTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC TCGGATGAATTCTCCGTTAAGTTCTAAGAATCTGAGAATCTTTTCAAAGTCTGTTGCTTGCAAATGTCTTGTTCTTGTTG GTATTGCTCTGTTCTACAGAGCTCTGTTACTCTCTTACTCTCCCAGAAACGCTCTGAGCAATAGCTTATTGTTTCGAGAT CGTCACATGTCAGATTCCTCCTCAACCGGTGGAATCCGAACTGATAAGTTTCTCGAGGTTCCTCAGATCGTCTGGGGACT AAACAATCAGAAGATAGCTTTTGCTAGAGCTTGCTTGACGGCAAGAATGATGAACAGAACGCTTCTCATGCCTAGTCTAA GTGCTTCCTTGTTCTACAAGGAAGTTGATAAGCTTCGTCCCATTCCGTTTGATAAAGTGTTTCAGTTTGAAAGATTCAAC TCTCTCTGTTCAGGGTTTGTGCGGCTTTCTCGGTTCTCGGATGTTAAAAACAGAGCACAAGTGTTTGATCTAGAGAAAGG TAGTGGAAGAAGATGGACGGTTGAAAGAGACTTGGAACACTTGAAACAATCCGCTCGTAACGAGTCCATCGATGAGTTTG AAGTGATCCGTCTCATAGGGAAAAACCCGTTCTTGTGGCACGATCATTGGCCTGTTGAAGACTATGCTAAGGTCTTTGAG TGTATGGTGGTGGTTGATGAAATCTCAAGAGAAGCAGATAAGGTTGTGATGAAAATCCGAGAAGCAGGTGAGGCAGAAAG GGCAAAACTCGCATCGAAAACCGAAATCCTCGGTCCGGTACCTTTTGTTGCGGTTCACATGAGAATAGAGATAGATTGGA TGATACATTGTAAGAAACTAGAGCAACGGAAAAAGATCTCAGAGATTTGTAGTAGTAAAAGAGAGATTTTGGACAGAGTA GGGAACATTTCAGGTTTAAAGACTCCAACAGTTCTTTACCTTGCAGTAGCAGATACTCTCTTAGAAGAAAAAGAAGAAGA TTCATCGGTTTTAAGTGGTTGGAGAGACGGTTTAATACCATTTGAGAAGAAGAAGCTTGGGGTTAAAGAGGAGATATATG GGAAGTATTCATATTTGATACAATCGGCTATAGATTACGAGGTGTGTTTAAGAGCGGATGTTTTTGTTGGTAATAGCTTT TCGACTTTCTCTAGTCTCATTGTTCTTGAGAGGACACAGAAAGCGAGAAGGTTAGGGTTTATGAGTTCTTGTAAAGATGG TGGAAACAAGTGGAGATCTTATGCATATAATCTGGCTGGTGAATCAAACGGAGTTCCAAGGAGATGGATGACAAATATGA CTCATTCGAGCTTGCAAGCTATTAGCTATGGCTCAAATTCTGTTTCTTGTTCTAGTGGATGAACTGGCATTTTAGGTCTA GTTTTTTTTGGCTTTTCTATACAATTCGTTTATTTGTGGGGTAAAAAAAAGTTGTGTAAATTTTACAGGAAAAAGAACAA .............N.................................................................. ................................................................................ ....i..........................................................i................ ................................................................................ ......i......................................................................... ..............................................N..i.................i............ ................................................................................ ........................................i....................................... ............i..........................................................N........ ...............................................................N................ ...i............N...............................i............................... ..........................................................N....................N ................................................................................ ................................................................................ .............................................................................N.. .........................................................N...................... ............................................................N...............i... ....................N..........................................N...N........N... ...........................N..............................N..................... ................................................................................ Pos Score Pred ------------------------ 14 0.377 - 165 0.574 Yes 224 0.614 Yes 327 0.805 Yes 447 0.425 - 450 0.707 Yes 468 0.581 Yes 601 0.529 Yes 653 0.651 Yes 712 0.090 - 784 0.206 - 804 0.632 Yes 817 0.237 - 849 0.656 Yes 939 0.294 - 960 0.284 - 1198 0.293 - 1258 0.148 - 1341 0.284 - 1357 0.537 Yes 1381 0.134 - 1424 0.202 - 1428 0.173 - 1437 0.136 - 1468 0.340 - 1499 0.272 -

Explain the output -- Processing locus 560: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATTGACCCGTTTTGTAACATATATACTAGAGTGAGTAGTGATAATTTATAGAAATTTGATATGTATATGTCTCTGAGTA GTTAGTGAAATTGTAACATAAGAGATTGGGCCTGACTTTATTAAGTTAGGCCCATTAATGTCTATCACTAACACGAAAAC CAGCATCTGCTTCAGCTCGTCGTCTTCTCTACCTGAATCAGTGCGTCTGGCTCAGGTGACAATGCAAGGAGGATCATCGG GTATTGGATACGGTTTGAAGTATCAGTCTAGATGTATATCGGATGTGAAAGCCGATACGGATTACACAAGCTTTCTCACT GGAACTCTTAGTTTAAAAGAAGAAAATGAGGTTCATCTTCTTCGTTTGTCATCTGGTGGATCTGAGCTTATATGTGAGGG TTTGTTCTCTCATCCTAATGAGATTTGGGACCTTGCTTCTTGCCCTTTTGATCAACGCATTTTCTCCACCGTCTTCTCCA CTGGTGAATCGTTTGGAGCAGCGATATGGCAAATTCCTGAGTTATATGGTCAACTGAATTCTCCACAATTGGAGCGTGTT GCTTCTCTTGATGCTCATGTTGGTAAGATTAATTGTGTTCTTTGGTGGCCTTCTGGAAGATGTGACAAGTTAATCAGCAT TGATGAACAAAATATTTTCTTGTGGAGCTTAGACTGTTCGAAAAAGTCTGCTGAGGTTCTATCTAAGGATTCAGCTGGGA TGTTGCATTCTTTATCTGGTGGAGCATGGGATCCACATGATGTAAATGCTGTTGCAGCAACGGGTGAATCATCTGTTCAG TTCTGGGACTTGCGTACTATGAAGAAGGTTAACTCAATTGAACATGCTCATGTACGCGGTGTTGATTACAATCCCAAGAG AGAACATATACTTGTAACTGCAGAGGATGAATCTGGCATACATGTCTGGGATCTCCGGAAGGCCAAGGTTCCTGTCCAAG AGCTTCCTGGTCATACACATTGGACTTGGGCTGTCAGGTGTAACCCTGAGTATGATGGACTGATTCTGAGCGCTGGAACA GATTCGGTTGTCAACCTGTGGTATGCTTCAGCAACATCCAGTGATGACAAAACCTCAGAAAGTCCTGTGGAATCCACACG ACAGCGTGTGAACCCGTTGCTCAACTCATACACTGACTATGAAGACAGTGTCTATGGCCTTGCTTGGAGTTCACGTGAGC CTTGGGTTTTTGCATCATTATCATATGACGGAAGAGTCATCATCGAATCCGTCAAGCCTTTCCTTCCAAGAATATAGTAA AAACCCAGCATCTCTTTTGCTCTGACCCAGACACGACCCATCTTTTGGTAAAGTTGGCACTATTGTATTTGAAACAATGT ACTTTTGACACAATCT .............................................................i.....N............ .........................................................N...................... .............................................................i.................. ...............................N..........N..................................... .........................i.............................................N........ .................N.............................................................. .........................N...................N.................................. ..........N.....N..........................................N.................... ..N............................................................................N .........................N..........N..N.....N.................................. ..................i........................i.....N.............................. ..........................N..............N...................................... ...................................................N..N......................... ......................N....................N.................................... ......................................N..............i.......................... ........................N....................................................... ............................................................................N... ................ Pos Score Pred ------------------------ 62 0.555 Yes 68 0.439 - 138 0.425 - 222 0.523 Yes 272 0.180 - 283 0.271 - 346 0.541 Yes 392 0.128 - 418 0.179 - 506 0.344 - 526 0.295 - 571 0.203 - 577 0.079 - 620 0.192 - 643 0.214 - 720 0.335 - 746 0.438 - 757 0.245 - 760 0.100 - 766 0.104 - 819 0.522 Yes 844 0.510 Yes 850 0.327 - 907 0.353 - 922 0.454 - 1012 0.258 - 1015 0.247 - 1063 0.173 - 1084 0.292 - 1159 0.385 - 1174 0.543 Yes 1225 0.317 - 1357 0.071 -

Explain the output -- Processing locus 561: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGCAAGTGTTTAACTTTGGGAGAGGAAGATGAGAATTACAAGTGTTGTTATGGTTATTGGCTATGAGACTTGTCTCTGG TGTGAGCTGAGATGGTGGTAGATACTTTGTTTTAACGTCTTGGCTATGGAGAATCATCATCCTTCTACTCTATTGTCTAT GGATTCAAGTGCTTCTTCTCATGAGGAGCTTGATTTGGAGATGAATAACAACAGGCAATCTCTGCTCTCTGGCCCTCCTG ATATCAATTTACCTCTCTCAGCTGAGAGAAGTCCTCCTCCTCCGCCGTGGAATTTAGATGCTTGTGATATTTTGGATGTT GGGTTAGGTTCACAAGCTTATGAGACTGAGAATTACATGAGTGTTGTACCTAAGGTTGGGAGGAAATGTGCTAAACGTGT TGATAGTATCTGGGGTGCTTGGTTTTTCTTCAGTTTCTACTTCAAGCCTGCGTTAAACGAGAAATCAAAGGCGAAAATCG TCCGGGATAGTAATGGGATATCTGGTTTTGACAAGTCGGATTTGAAGCTTGATGTGTTTTTAGTTCAGCATGATATGGAG AATATGTATATGTGGGTGTTTAAGGAAAGACCTGAGAATGCACTAGGGAAGATGCAGTTGAGGAGTTACATGAATGGTCA TTCTAGACAAGGGGATCGTTTGTTTCCATTTAGTGTTGAGAAAGGGTTTGTTAGGTCTCACAGAATGCAGAGGAAACACT ACAGAGGTTTGTCGAATCCGCAGTGCGTTCATGGGATTGAGCTTGTTCCTTTGCCAAACCTCACGTGTCTAGATGAAGAA GAGAGGAAAAGGTGGATGGAGCTTACGGGTCGTGATTTGAATTTCACCATTCCACCTGAAGCTAGTGACTTTGGTTCTTG GAGAAACCTTCCCAACACTGACTTTGAGCTTGAGAGGCCTACACCTTCTTTGAAGAATCCTACTGCAAACCACTCTAAGA AACTACTCAATGGCTCGGGGCTCAATCTCTCGACTCAACCATCCAACCATTCTAATGGTGAAGCAACCGATTTATCTCCT TCGAGCCACAAGAAGAGGAAGGACTTGTTCTCAAATGGGATTCATGAGGAGGAGTGTTGCTTAACTGTAAACCCACAACC TCCTGTTATCGAAGCTCACCAGAATGAGCTACCTACTTGGTCAAACGAGTTTACTGGGGCCATGAAAAACGTTTATGGAC CTGTTACTGCTGCAAAAACGATCTATGAGGACGAAGAAGGCTACTTGATCATAATCAGCCTTCCTTTTGTGGATTTGAAC AGCGTTAAAGTCTCCTGGAGGAACACTCTCACACATGGAATCATAAAAGTTTCATGCCTCAGCACATCGCGCGTACCTTT CATAAAGAGACATGATAGAACATTCAAGTTGACTGATTCAGCCTCTGAGCATTGCCCGCCAGGTGAATTTGTCAGAGAGA TCCCATTATCAAACCGAATCCCGGAAGATGCAAACATTGAAGCGTATTACGATGGACCTGGATCAGTTCTTGAGATACTT GTCCCAAAACTAAGAGCTGGCCCGGAAGAGCACGAGGTGCGGGTGTGTCTCCGTCCCAACCTCGGTGGAAACGATCTTAT GCTGACTTAAAAAGAGAAACAACTGTTTAGTTTTCTTGTTTGTAGAAGCTTATTATAGGAGTCATCTCTCATTGATATTG GCCACTCATATATAGGCGTTTTCACTGTT .............................i....................N............N................ ...........N.................................i................................N. ....................N...................i....................................... .........................................................N.................N.... ...................N................N............................N.............. ................................................................................ ............N......................................N.................N....N..... ...N.....N...........................N.............i.................N...N...... ................................................................i............... ..............................N.........................................N....... ...............i................................................................ ................................................................................ .........N............................................i......................... ..................................N........N.................................... .......................N.....................................i............N..... ........................N....................................................... ..................................N..................N.......................... ...........i.................................................................... ...........................i.......................N............................ ..............................................................................N. ................................................................................ ............................. Pos Score Pred ------------------------ 30 0.566 Yes 51 0.259 - 64 0.088 - 92 0.265 - 126 0.681 Yes 159 0.293 - 181 0.143 - 201 0.574 Yes 298 0.291 - 316 0.219 - 340 0.161 - 357 0.366 - 386 0.236 - 493 0.361 - 532 0.114 - 550 0.115 - 555 0.260 - 564 0.223 - 570 0.317 - 598 0.451 - 612 0.631 Yes 630 0.210 - 634 0.264 - 705 0.817 Yes 751 0.091 - 793 0.281 - 816 0.706 Yes 970 0.345 - 1015 0.791 Yes 1075 0.402 - 1084 0.220 - 1144 0.281 - 1182 0.688 Yes 1195 0.229 - 1225 0.242 - 1315 0.355 - 1334 0.302 - 1372 0.626 Yes 1468 0.630 Yes 1492 0.490 - 1599 0.294 -

Explain the output -- Processing locus 562: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATGTGGCGTTATCTAATCACATGAATGATTGAATAAAAACCAGGATCTTTGTTACTTTTATTTCAAAAGTTTTCTTTG GTATGTTCTTTCTCCGATTAAAGAGTTTCTTTGATAAAAAATATTTCAACAAACTTTATTGTACTCGCCGCGGATCGAAA CATCGTAATTCTTATCTACTGCACAAGCACAAGGATGGAAAAAATTAGGTTCCCGGTAACGAAACCGGCATTAAATTGGG CCGAAATTGGGCTGAAGCCCATATATGAAGACCAGCAACACAAAAGCAGAAAGAGAAGCGTGGTTGGATTTGTGTGTGCT GTGCCACACTCTCCACTCTTTAGGGTTGCGACGGAGTCTTCAATGGGGAGAATAGGGAGTCCACTTGGTAAGAACGAGTT CAGTTGGGTAATCAAGGATTTCTCCTCTTTGGGAGTGAGGGCAATTTATTCTGACGAATTTGTGATCGGTGGCTGCAAAT GGCGTCTTATAGCCTATCCCATGGGAAATAGGATTAAAAAGTATATGTCTTTGTATGTGGAAGTTGCTGATTCTAAACAT TTGCCTTCTGGATGGAGTATAAACACAGAATTGCGCATGGAAGTTGTAAATCACAATTTGTATAAGCCCTCCCAACAGAA ATATAGAAAAAATTTGTGGTTTGATCAGAAGACTCCTTCCTGGGGTTATAAAACCATGATTCGTCATTCTAAACTTTCTG GTGAAGAAGGGTTTTTGGTAAGTGGAGAAGTCACAATTGTAGTCAAGATAGACGTTTATAGAGTTTTTGGCAAAGTAGCT GCAATAGAGATTTCTGAAGAGGGATCTAAAGAGGGATATGAATATGAATCTGAAGAGGTATATAAAAAGGAATCTGAAGA GGGATATAAATATGAATCTGAAGAGGATTACGAAAAGAGACCTATAGAGAGATTTGAATATGAATCTGAAGCGGAATCTA AAAAGGACTACGAAGAGAGAACTAGAGGGGGATTTGAATATGAATCTGAACCGGTATCTAAAAAGGATTACGAAGAGAGA CCTAGAGAGAGTTTTGAATATGAATCTGAAGCGGAATCTAAAAAGGATTCCGAAGAGGGATCTAGAGAGAGATATGAATA TGAATCTGAAGAGGAATCTAAAAAGGATTCTGAGAATGATTGTGGAGAGGAATCTGAAAAGGATTCTGAAGAGGGAAGCA GACCTCTTAAAAAGATTAAGAGGAATGATGATGGTGTGAAGTCTAATGATTCAGTCAACAAGACTCAACAAGTGAAGGAA ACCATGGATATCAATGGGTTTCAAGTTCTTCCTTCACAGGTAAGTGGAAATTTCAAACTCTTGAGACATTGTTATCATTT GACTCTCGTTGATTTTGGGACAAAAGGTGTAACACTTCTAAATACAGAGAAAATGTAACTTTTTGTGAGTTTGAGAGTCA ACCCCACCCTTGTGTTTCTGGATATGTATACATATGGGTTTTACTTTTACCCTTCCTTCTCAATCGCAGGTGACACT ..N...................N...N..................................................... ..N............................................................................. ..................................i............................................. ........................N....................................................... ..........................................N..................................... ..............................................................................N. ....................i.......................i.........N......................... ...........N........................N........................................... .......................................................i........................ ................................................................................ .....................................N.....N.................................... ...........N...............................................i.................... .......................................i........................................ ...................N.....................................................N.....N ...................................N............................................ ........................N..N..N..............N.................................. ...i.........N.................................................................. ....................................................N........................... .......................N.........N........................................... Pos Score Pred ------------------------ 3 0.158 - 23 0.259 - 27 0.190 - 83 0.173 - 195 0.561 Yes 265 0.225 - 363 0.247 - 479 0.398 - 501 0.627 Yes 525 0.582 Yes 535 0.151 - 572 0.487 - 597 0.384 - 696 0.697 Yes 838 0.155 - 844 0.236 - 892 0.430 - 940 0.598 Yes 1000 0.500 - 1060 0.287 - 1114 0.133 - 1120 0.293 - 1156 0.268 - 1225 0.215 - 1228 0.253 - 1231 0.168 - 1246 0.143 - 1284 0.573 Yes 1294 0.352 - 1413 0.276 - 1464 0.087 - 1474 0.075 -

Explain the output -- Processing locus 563: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGAGCTGGTCCTAATTCTGATTCTGATAAAGATTTGATTTTTATGGTTTCTTTTGTAGGTTTTGTTGTTCTCTTAATTG AAAAACAAGATCTTGAACTAAAAAATGGAAACTGAAGACGATTTGTGCAACACCAATTGGGGAAGCTCTTCTTCGAAATC GCGAGAGCCGGGTTCATCAGACTGTGGGAATTCGACATTCGCAGGTTTTACGTCCCAGCAGAAGTGGGAAGATGCTTCAA TCTTGGATTATGAGATGGGTGTGGAGCCTGGATTGCAAGAGAGCATTCAAGCAAATGTTGATTTCTTACAAGGTGTTAGG GCTCAAGCGTGGGATCCAAGGACGATGTTGAGTAACCTTTCTTTTATGGAGCAGAAGATTCACCAGCTGCAGGATCTTGT TCATCTTCTTGTTGGTCGTGGTGGGCAGCTTCAAGGTCGTCAAGACGAGCTCGCGGCTCAGCAGCAGCAGCTTATAACGA CGGATCTTACTTCCATTATCATACAGCTTATTTCAACTGCAGGTAGTCTTCTTCCATCTGTTAAGCATAATATGTCAACA GCTCCGGGTCCATTCACTGGGCAGCCCGGTTCAGCCGTGTTCCCTTATGTAAGGGAGGCTAATAACGTTGCTTCACAGAG TCAGAACAATAACAACTGTGGTGCTCGAGAGTTCGATTTGCCTAAGCCGGTTCTTGTTGATGAGAGGGAAGGTCATGTTG TGGAGGAACATGAGATGAAAGATGAAGATGATGTGGAAGAAGGAGAGAACCTTCCTCCCGGTTCGTATGAGATATTGCAG CTTGAGAAAGAAGAGATTCTCGCACCGCATACTCACTTCTGCACGATATGTGGCAAGGGTTTCAAGAGAGACGCGAATTT GAGGATGCATATGAGAGGGCATGGAGATGAGTACAAAACAGCAGCTGCTCTGGCGAAACCGAACAAAGAATCCGTACCCG GGTCTGAGCCGATGCTGATCAAGAGGTACTCGTGCCCATTCCTTGGTTGCAAACGTAACAAGGAGCACAAAAAGTTCCAG CCTTTGAAAACGATTCTATGCGTGAAGAATCACTATAAACGCACCCACTGCGATAAAAGCTTCACTTGCAGCCGGTGCCA TACCAAGAAATTCTCTGTCATTGCAGATCTTAAAACCCACGAGAAGCACTGCGGGAAAAACAAGTGGCTTTGTTCCTGTG GTACAACATTTTCAAGGAAAGACAAGTTGTTTGGTCACATAGCTCTGTTCCAGGGACACACGCCTGCAATCCCTCTTGAA GAGACAAAACCATCAGCCAGTACATCTACTCAGAGAGGGAGCTCTGAAGGCGGGAACAACAACCAAGGAATGGTTGGCTT CAATCTTGGTTCTGCATCAAATGCTAACCAAGAAACCACACAGCCTGGAATGACTGATGGGAGGATATGTTTTGAGGAGT CGTTCTCACCAATGAACTTTGATACATGTAATTTTGGAGGGTTCCACGAGTTTCCGCGACTGATGTTTGATGATTCAGAG AGTTCTTTTCAAATGCTTATTGCGAATGCCTGTGGTTTCTCGCCCAGGAATGTTGGTGAGTCTGTTTCAGATACTAGTCT CTAAGGCATTGCCATATATATGATAAGTTATGTAAGATGTTCTAGTTTTTAGCCTTTTAAGCAGTTCTTTTGTGTCTGTA ATTTTGTTTTCGCCATAATAATA ...........................................i.................................... ........................i....................................................... .......................................................................N........ .........N....i.......................................N......................... ........................i....................N.................................. ................................................................................ .......................................................................i........ ..............................................N................................. ...........................................................N..............N..... .........N....i......N.....N..N...................................N............. ...............................................N................................ ....i.....i.........N.....N..................................................... ...........N.................................................................... .................N.............................................................. ................................................................................ ................................................................................ .....................................................................i.......... ....................N............................N......N.........N............. ...........N.............N....................................i......N.......... ............N............N.......................N.............................. ...................N.........N......N........................................... ....................... Pos Score Pred ------------------------ 44 0.691 Yes 105 0.729 Yes 232 0.262 - 250 0.280 - 255 0.755 Yes 295 0.378 - 345 0.554 Yes 366 0.391 - 552 0.868 Yes 607 0.397 - 700 0.135 - 715 0.132 - 730 0.150 - 735 0.640 Yes 742 0.167 - 748 0.388 - 751 0.286 - 787 0.105 - 848 0.258 - 885 0.533 Yes 891 0.580 Yes 901 0.372 - 907 0.306 - 972 0.251 - 1058 0.412 - 1350 0.831 Yes 1381 0.084 - 1410 0.355 - 1417 0.433 - 1427 0.362 - 1452 0.383 - 1466 0.425 - 1503 0.589 Yes 1510 0.179 - 1533 0.322 - 1546 0.118 - 1570 0.119 - 1620 0.075 - 1630 0.054 - 1637 0.085 -

Explain the output -- Processing locus 564: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATCATTTCTTTGAATGAGTTTGTTTTAATTTTGTGTTAGGGTTATAACGCGATTGGGAATTTAGTTGAATTTAGAAC TAAATTTGTGCTAGCTTGTGATTTATTGTCTCTAATTTTGAACTACTAATATTTGATTACCATATGTTTAGTGGTACAGA AGAAGAGAAAGGAATGATGATTAGTGAAAGAGATGATTTTAGTCTCACTGGACCATTACACTTAACATCTATAGATTGGG CTAATGAACATCATCGACGATCCGTAGCTGGATCTTTGGTTCAAGGAATCTATGTAGCTGAGCGTGACCGTCAGCTACAA AGAGAAGGTCCTGAGTTAGCTTTATCTCCAATATGGTCTGAGTTTTTCCATTTCCGCCTCATTCGTAAGTTTGTCGATGA CGCGGATAACTCTATCTTCGGAGGAATCTATGAGTACAAACTGCCGCAACAGCTCTCTCAAACCGTCAAATCAATGGAAT TTAGTCCACGTTTTGTGATTGCTTTCAGAGGAACGGTTACAAAAGTGGACTCCATTTCCCGTGACATCGAGCATGACATC CATGTTATTAGAAACGGGCTTCACACGACAACACGGTTTGAGATAGCTATCCAAGCAGTGAGAAACATTGTTGCTTCGGT TGGTGGTTCTAGTGTTTGGCTTGCTGGTCATTCTCTTGGTGCATCTATGGCATTACTTACCGGGAAAACCATCGCTAGAA CCGGGTTTTTTCCTGAGTGTTTCGCATTCAATCCGCCGTTTTTGTCTGCCCCTATCGAAAAAATTAAGGATAAGAGGATT AAACATGGGATACGCATTGCAGGCAGTGTGATCACAGCTGGACTTGCTCTAGCCAAAAAAGCCACCCAACACTACAGCCA AAACGACCGTGCATTACCCGCACCTCCTGATCCATTTGAAGCTTTATCCGACTGGTTCCCGCGGCTGTATGTCAACCCTG GTGACCACTTATGCTCAGAGTATGTTGGTTACTTTGAGCACCGAAACAAGATGGAAGAAATCGGGATTGGGTTTGTAGAG CGGGTAGCGACGCAGCACTCGTTGGGCGGTATGCTGTTAGGAGGACAAGAGCCGGTACATCTGATTCCATCTTCGGTTTT GACGGTGAACTTAAGCTCCTCGAGAGATTTCAAACAAGCTCATGGGATTCATCAGTGGTGGAGGGAAGATAACAAGTTTG AGACTAAAGTTTACCAGTACAAATGATTTGTATCAGTTTTTTTTTAAATGGATTTAACCGGTATTAGGCTTTTACTTTCC GGTTTAGACGGTTCAATGTATCGCAATCATAAATAAGTGTCTCTC ................N............................................................... ...............................................................N................ .............N..i...............N............................................... ...N...............................................N............................ ................................N...........................................N... .............................N...........................................i...... ........................................................................N....... ..............................................N................................. ................................................................................ ....i........................................................................... ....................................................................N........... ..........i..........N............................i............................. ..............................N................................................. .........................................N...................................... ......................N........................N................................ ...............N............................. Pos Score Pred ------------------------ 17 0.177 - 144 0.308 - 174 0.489 - 177 0.752 Yes 193 0.247 - 244 0.154 - 292 0.267 - 353 0.357 - 397 0.300 - 430 0.179 - 474 0.644 Yes 553 0.233 - 562 0.090 - 687 0.420 - 805 0.793 Yes 949 0.263 - 971 0.544 Yes 982 0.418 - 1011 0.671 Yes 1071 0.176 - 1162 0.276 - 1223 0.229 - 1248 0.084 - 1296 0.059 -

Explain the output -- Processing locus 565: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAACAAAAAAAAGATAATATTGTTTTCCCGACGACTAATCCACTTTCAGTATTTTATAAAATAACTGAACTCAAGTTGCT CGATCAAACAAGTACCCTTAAATGTCTCTCTGTCTATATAAATGTACACTCTTCCCAAACAAAACCATATCTCAAAATAT GAATAATCAAAAATGGAGTATAGGTTTCATATCTCTCGCTTTTCTCTTCATCACTTCCTCTTCAGCTGAGTTCATCATTC AACAGGTCACAAAGGGCAGAGGAATAGAGTACAACAGTTCTTACAGTCTCGAGGAGAATCTTGGAGTGACAAGAGAGTTG AGAGAAGAGCGACCATCGAGTAAGATAGTGACAATAACAAGCTTCTCAGTGATTAAAGGCAGAGGAGAACCCTATGAATC CTCTGTTTTTGAGGCTGCTGGTTACAAATGGAGATTGGTTTTGTACGTGAATGGTAATAAAAACGACGGTGGAAATGATC ATATTTCCCTTTACGCAAGGATCGAAGAGACAAACTCTCTTCCATTAGGTTGGGAAGTGAATGTTGATCTCAAACTCTTT GTCCATAATGGGAAGCTACACAAATATTTGACTGTTACAGATGGCTTAGTGAAGCGATATAACAATGCGAAAAAAGAATG GGGTTTCGGACAATTGATTCCTCGATCAACATTCTACAACGCGAACGAAGGTTACCTTGACCAGGACACTGGTTCTTTTG GTGCTGAGATCTTTATTGTTAAACCGGCTCAACAACAAGAGAAAGTTACATTCATATCAAACCCTCCAAACAATGTTTTC ACTTGGAAGATACTTCGTTTCTCTACCTTGGAAGATAAATTCTATTACTCCGATGATTTTCTCGTTGAAGACCGATACTG GAGACTAGGATTTAACCCGAAAGGGGATGGAGGAGGAAGACCACATGCACTTCCAATCTTCCTATTTGCTCAAGGCCATA AGGCAAATGCAGTTGCTACAAACACTTGGGGAGCGGTTAATCTGCGGTTAAAGAATCAACGAAGTACTAACCATAGACAA ATATATTCTGCAGCTTGGTACCCGATTGGAAGCGGTTATGGTGTGGGAGTGAACAATATCATACTGTTAGCTGATTTAAA CGATGCATCAAAAGGATATTTGGTGAATGATGCCATTATCTTTGAAGCTGAAATGGTTAAGGTCTCTATAACCAACATCG TCTCCGCTTAAATATCTGCACTTCTTTGTCTACGATCAATCAACCTTATGAATAAAAAGATGTTTAATTTGATGAGTTTG TAATAAGAAAACGTTAATGTTTGTGAATTG ................................................................................ .....................i...................N....................................i. ............i................................................................... ................................................................................ .........................................................................N...... ...........................N......................N.......................N..... ............................................................N................... .......N................................i.......................N............N.. ................................................................................ ........................................................................i....... ....................................................N........................... ..........................N.................N................................... ......N......................................................................... .....................................N.......................................... ..N.......................N..N......................i........................... ...............................................N...........N...........N........ ................N............. Pos Score Pred ------------------------ 102 0.568 Yes 122 0.332 - 159 0.639 Yes 173 0.918 Yes 394 0.157 - 428 0.417 - 451 0.352 - 475 0.246 - 541 0.273 - 568 0.365 - 601 0.648 Yes 625 0.424 - 638 0.387 - 793 0.547 Yes 853 0.483 - 907 0.354 - 925 0.285 - 967 0.375 - 1078 0.174 - 1123 0.153 - 1147 0.101 - 1150 0.150 - 1173 0.539 Yes 1248 0.056 - 1260 0.071 - 1272 0.053 - 1297 0.096 -

Explain the output -- Processing locus 566: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACACTGAAAACTTGAAACTCATTTTGAGAACTATTGCCATTCATAGCCTAAGCATCTAGAGAAATTTTTAGTGATTCTT GAAATCTTTGTCTGTTTTTGACAAGAAACGCTTGAATCTAGTACCACAAATATGTTTCTCCGTCTTCTTAAACCTTGTAT ATATAGCTTTGCTATTGACACTAAACCGATGAAAACTCTACACAGGACTTGGATAGTGGATGGCCCATGGATCTCAAGGA ATGTAAAGAATGCGTCTCTGTCTTCAGCTCTGCAGATTAAAGACTGCGGAGCTTATATCAACTGCTTTAACTGCTCTTAC CGCATCGACAACAGCAATGTACTGACTCCATGGCCAGGCCTTCCTAAAGGTGTGAAATTCGAGCCAACCGATGAGGAAGT GATTGAGCATTTGGAAGCTAAGTGTGGTATTGATGGCTTAAAACCACATCTTCTTATCCAAGACTTTATATGTTCAGTCA CTCAAGATGTTGGTATCAACTACACTCACCCTCAAAACCTTCCAGGTGTGAGTAAAGATGGAACTAGTGTCTTCTTCTTT AACAAGACGGCACATGCATATCAAAATGGGCAGAGAAAGCGGAGAAGGATCACACCAACTAGTCTCAAGGATGATACGGT TCGCTGGCACAAGACTGGTCAAACCAAACCGGTGATGCTCAATGGAATCCAGAAGGGATGCAAGAAGATCATGGTGCTCT ACAAGAGCGCGAGAAAGGGATTCAAACCCGAGAAATCTAACTGGGTTTTGCACCAGTACCATTTGGGAACTGAAGAAGGC GAGATTGGGGAATATGTTGTCTCTAAGATCACATATCAGCAACCAAAGCAACAGGAGAAAACCATCGATGAGAGTGAGAG TTCTGGGGTTCGAGGTGGCCCTAGCACACCGAAAACTAGTACTATTACACAAGTTAGACCTGTGATTTCTGTTGATGAGG ATGAAATAGCTTTTGATGATGATTCTAAGATGGTTCTTGATTCATATGCCGAGGGACTGGAGAACATTCAAGAAGCTTCT TCTGGTTCGACATCAGACAAAATAGCTAAAGTGGGAGGGAATGTGAGTGTTATTGAAGATAACTTAATGTCCAAGAAAAT TGAAGCTTCGTCGATTCCTAATCATGGAAATGTTGACTACGGATCAGGAAACTTTTCAGTTTCAGATTTGGAGAATGCAG AATTAGGGACTCTCCCTGATTTACTTTCCGTGAGAACATAAAACACACACACACTTACTTTATATATTTAGAAAATCATT GAGAAACTTAAAACTAAGATCTTTTGCGGCTTTTGCAGTTTGCTTCTGAGGACAGCTTAA ................................................................................ ...................................................i............................ ............................N..............................N......N............. i........i...................................................................... ................N............i........................................N......... ................................N....................................N.......... ......i..................................................N...................... .............N...........N............................................N......... ..................................N......N...............N............i......... ................................................................................ .............N.....................................................N............ ..........................................................................N..... N..............N..N..........i...............N.................................. ........................................N.........................i............. .......................N.....N............................................N..... ................................................................................ ............................................................ Pos Score Pred ------------------------ 132 0.535 Yes 189 0.468 - 220 0.184 - 227 0.457 - 241 0.561 Yes 250 0.631 Yes 337 0.362 - 350 0.570 Yes 391 0.302 - 433 0.320 - 470 0.215 - 487 0.584 Yes 538 0.406 - 574 0.228 - 586 0.409 - 631 0.322 - 675 0.383 - 682 0.253 - 698 0.434 - 711 0.704 Yes 814 0.364 - 868 0.195 - 955 0.265 - 961 0.132 - 976 0.262 - 979 0.326 - 990 0.760 Yes 1006 0.196 - 1081 0.282 - 1107 0.742 Yes 1144 0.246 - 1150 0.252 - 1195 0.175 -

Explain the output -- Processing locus 567: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAACACATACAGGGAACTTTGCTTGGCCACCCAGACATATTATTATTTCAATAATTATTCAATTGCTAAAAAATATTAG AAACTCAACGGGCATATATGAAGTATGGAGCCTCAGTTTCTATATAAAGCAACTCTGCCAAGTGCATTCCTCATATACAA AAGGAAGAAACACTTAAAAGAAAATATCAAATCTAAAGTCGATCACATGGCTAATAAGAGTTTAGAGATTCGGTTTTTAT TTCCACTTGTTTTGTTTTTGGTTGTGAAATTACTTTGTGTTGATGGTAAAGGATTCAATAATAATGGCCATAAGATAAGA AAAGGAAGATGGGAAGGTAAGCTGAAGATGAACTTCTATCACAATAGCTGCCCTGGAGCCGAGGATATCGTGAGGCAGAT AGTGTGGAAGAAAGTCGAAGCAAACCGTAGCCTGGCTCCCAAGTTACTGAGGGTTCATTACCATGACTGCTTTGTTAGGG GGTGCGACGCATCGCTGCTTTTAGATTCGGTTGCTGGAAAAGCAGTGTCCGAGAAGGAAGCGAGACCTAATCTCTCGCTT TCCGGGTTTGAAATCATCGATGAGATCAAGTATATTCTCGAAAAACGATGCCCTAACACCGTCTCTTGCGCTGACATTCT CACTTTAGCCGCCCGAGATGCCGTCTCTTATGAATTTGAACGGCCGCTATGGAATGTGTTTACCGGACGTGTTGATGGAA GAGTTTCATTGGCAACGGAGGCGGCCAGAGACTTACCATCCGCTGGGGCAAACTTCACCACTCTACAGAAACTCTTTGCC GAGAGCGATTTGGACGTCGTTGACCTCGTCGCTTTATCAGGAGCACACACGATAGGAATAGCACATTGCGGTGTGTTTGG CAGAAGGCTCTTGAACTTCACCGGAAAAGGGGACACAGATCCTTCCCTAAACCCTAGTTACGCCTCCTTCCTCAAATCCG AATGCTCCGACAAATCTTTAAGACTCAACCCTTCCGCAGTGGTCGGAATGGACCCTACTGGTCCTCTCGCCTTTGACAGT GGCTACTTCGTCTCTCTACTCAAGAACAAAGGACTCTTCACCTCCGACGCCGCCTTGTTGACTGATCCCTCCGCCGCTCA CATTGCTAGTGTTTTCCAAAACTCCGGCGCTTTTCTTGCTCAGTTTGGTCGGTCCATGATAAAGATGAGTTCCATCAAAG TCCTTACTCTTGGAGATCAAGGTGGTGAGATTCGTAAAAATTGTCGCCTCGTCAACTGATAAATTTTCACTTTTTTTTTT ATCTTAATACCGTATAAATTTGATTATCATGTAATCATCATTTGAGATATGTTTTTCTTAATTTTATTACAATACTAA ................................................................................ .................i......i....................................................... ..............................................i................................. ..........................................N....................i................ ........i..................i.................................................... ..............................................................N................. ................................................................................ ...................N...........................N................................ .................i...........N..................i....N....................N..... ................................................................................ ................................................................................ ................................................................................ ................................................................................ .......................................................N........N............... ................................................................................ ............................N...................N............................. Pos Score Pred ------------------------ 98 0.511 Yes 105 0.660 Yes 207 0.514 Yes 283 0.449 - 304 0.552 Yes 329 0.834 Yes 348 0.500 Yes 463 0.496 - 580 0.221 - 608 0.374 - 658 0.555 Yes 670 0.366 - 689 0.772 Yes 694 0.474 - 715 0.467 - 962 0.308 - 1008 0.792 Yes 1176 0.280 - 1185 0.434 - 1309 0.056 - 1329 0.046 -

Explain the output -- Processing locus 568: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAATTAGTATGAGTTTTTTTTGGGATTTTTAACCACAAAAAAAATCTTTGACCTTTTAAAAAACTCTTATTGAAAAAA AAAAAAGGAGTTATATGATGAAACCAAATGGACCCATATGATTGGGAGATTTGGATAACAGAGAATCCACAAAAGTGTAT CCTTCACAAAACCAAAACACACTCTCCATTTTCTTCAGACAACTCTTTCTCCTTTCTTCTCTCTTCTCTGTCTCATCACA AACCAGAAATGGGAGGAGTGTCCTTCCTCTCCACTGTTCCTTCCTTCACCAACACAACCAATCATCAACATCTCACTACA CTCTCTTCTTCTTCTCATCGCTCTGCTGTTATACGATGTTCCAAGATTGAACCTCAAGTTTCCGGTGAAAGTTTAGCTTT CCACCGCCGTGATGTTCTCAAACTCGCCGGCACTGCAGTCGGAATGGAATTAATAGGCAACGGTTTCATTAACAATGTGG GTGATGCAAAAGCTGCTGACTTGAATCAACGTAGACAACGATCCGAGTTTCAATCAAAGATCAAGATTTTGCTTTCAACC ACCATTAAGGCTAAACCGGAGCTTGTACCTTCCTTACTCAAACTAGCTCTCAATGATGCTATGACTTATGATAAGGCTAC TAAATCCGGGGGAGCCAATGGATCCATACGGTTCAGCTCTGAGCTAAGTAGAGCAGAGAATGAGGGACTTTCTGATGGGT TGAGTTTGATAGAGGAAGTGAAGAAGGAGATTGACTCTATCTCGAAGGGAGGTCCTATTTCATATGCAGATATCATTCAA TTAGCAGGACAATCAGCGGTCAAGTTTACATATTTAGCATCGGCTATCCGAAAATGTGGTGGAAATGAAGAGAAGGGGAA CTTACTTTACACAGCATATGGTTCAGCTGGTCAGTGGGGATTGTTTGATAGAAATTTTGGAAGGAGTGATGCAACAGAGG CTGATCCAGAAGGCAGAGTCCCACAATGGGGGAAAGCAACTGTGCAAGAGATGAAAGATAAGTTCATTGCTGTTGGATTG GGTCCTCGTCAGCTAGCTGTAATGTCTGCATTCTTGGGTCCTGATCAAGCTGCAACAGAACAGCTCTTAGCTACTGACCC TCAAGTTGCTCCATGGGTTCAGAAGTATCAAAGAAGCCGTGAAACCGTATCTCAGACTGATTACGAGGTTGATCTCATAA CTGCATTCACAAAGCTAAGTTGCTTAGGACAGCAAATCAACTTCGAGGCATATACATATCCCGTCGAACGGATCAATCTG AGCAAACTCAAGCTATAAGCTATGTACACTATATGTATAACATGAGAGGAAACTTCGAAATAAACCAAAGATTTTTCTTG ACATTGAAACAAAAAGAGAAGTTGAATCCATTACAAA ..........i..................................................................... ..............N..N.........N.........N.......................................... ................................................................................ ........i....................................................................... ...................................i............................................ ...........N...............................i..............................i..... ...N............................................................................ ....................................................N..N....i......N............ .................N.........................................N..............N..... ...............................................................N................ .....................................................i..........N............... .................i..................................................N........... .........................i........................i............................. .....................i.......................................................... ............i................................................................... ................................................................................ .....................N..........N........N...................................... ..................................... Pos Score Pred ------------------------ 11 0.536 Yes 95 0.326 - 98 0.365 - 108 0.488 - 118 0.227 - 249 0.769 Yes 356 0.515 Yes 412 0.298 - 444 0.638 Yes 475 0.704 Yes 484 0.129 - 613 0.261 - 616 0.381 - 621 0.744 Yes 628 0.255 - 658 0.452 - 700 0.115 - 715 0.149 - 784 0.147 - 854 0.528 Yes 865 0.473 - 898 0.717 Yes 949 0.102 - 986 0.529 Yes 1011 0.623 Yes 1062 0.558 Yes 1133 0.676 Yes 1302 0.222 - 1313 0.083 - 1322 0.116 -

Explain the output -- Processing locus 569: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGCTATTTGTTAATTGTCCTTTAAATTTTGGCCACAATCAAAAGCTACAAAAAAAAAAGAGAGGATAACTGTTAGTTT GTAAACTAAATTCTCTCTTGAGATAAATGTTACGAAACGGCATTTTTTGTCATGTTACGTATCATAGTGTTTATCTTTGG GCCGGGTTTGGATATACCAAGCAAGACCATAAATTCAAGATCGTGTCTAGCTACGTGTAATACAAAAGTTCTTCTGACCA AAAGGATCATCAAACAAAGATCCTCCGAAACAATGGAGAAACCAAAGTTGAAGACAGTCCAAGAGGTGGTCGCAGCTGGC GAAAAACTACCGGAAAGATACCTCTACACACCCACCGGCGACGGCGAAGGTGATCAACCTTTCAATGGTTTGTTGCCAGA GATGAAAATTTCGATCATCGATCTTAATCTCCTTTTCTCTTCTTCTGACGACGGTCGAGAAGAGTTGAGTAAACTTCACT CGGCGATCTCTACATGGGGCGTTGTTCAGGTGATGAATCATGGAATTTCAGAAGCCTTGCTCGACAAGATTCACGAGCTA ACGAAGCAGTTCTTTGTACTTCCGACCAAAGAGAAACAGAAGTACGCAAGAGAGATAAGTAGTTTTCAAGGATTTGGAAA CGACATGATTCTGTCTGATGATCAAGTTCTCGATTGGGTCGACCGCTTGTACCTCATTACGTACCCTGAAGATCAACGAC AGCTTAAATTCTGGCCTGAAAATCCAAGCGGATTTAGGGAAACTTTACATGAATACACAATGAAGCAACAGTTAGTGGTT GAGAAATTCTTCAAGGCATTAGCTAGATCGTTGGAGTTGGAAGATAATTGCTTTCTAGAGATGCATGGTGAAAATGCTAC GTTGGAGACAAGATTCAACATCTACCCTCCATGTCCGAGGCCGGACAAGGTTCTTGGGCTAAAACCGCATTCTGATGGCT CAGCCTTTACTCTTATCCTACCCGACAAAAATGTAGAAGGACTTCAGTTCCTCAAAGATGGGAAGTGGTATAAAGCTTCA ATTCTCCCTCATACAATTCTGATCAATGTAGGAGATACTATGGAGGTAATGAGCAATGGAATATACAAGAGTCCGGTTCA TAGAGTGGTGCTTAACGGAAAAAAGGAAAGGATATATGTGGCAACATTTTGTAATGCTGATGAAGACAAAGAGATTCAAC CTTTAAATGGGCTTGTGTCTGAGGCTAGACCAAGGTTGTACAAAGCAGTTAAGAAGTCTGAGAAAAACTTCTTCGATTAC TATCAACAAGGTCGAAGACCGATCGAAGCTGCAATGATCTGAAGTTAAAAACATTGATCAGTCTGCTTTTGATATTCAAA ATACACATGTCTCTTTAGTTACTTGATCCTTGAAATAAGATCCCGTTTGCTTCTTTGTTGG ................................................................................ ..........................N........................N............................ ................................................................................ ................................i............................................... ................................................................N............... .............i..................N......N........................................ ................................................................................ ....i............N.............................................................. ................................................N..........i.................... ............................................................i...N........N...... ..............................N...........................................N..... ..............................i..........................N...................... .........................N.............i........i......N........................ ...................................N.................N.....N.................... ......N......................................................................... .................................N.............................................. ......N...................................................... Pos Score Pred ------------------------ 107 0.477 - 132 0.232 - 273 0.817 Yes 385 0.318 - 402 0.464 - 494 0.554 Yes 513 0.461 - 520 0.434 - 645 0.538 Yes 658 0.379 - 769 0.394 - 780 0.530 Yes 861 0.635 Yes 865 0.280 - 874 0.138 - 911 0.378 - 955 0.299 - 991 0.591 Yes 1018 0.446 - 1066 0.214 - 1080 0.523 Yes 1089 0.711 Yes 1096 0.270 - 1156 0.225 - 1174 0.220 - 1180 0.152 - 1207 0.372 - 1314 0.161 - 1367 0.056 -

Explain the output -- Processing locus 570: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCCTAAACCAAAAAAAAATCTAATTGTAAAGGTGTTGCAATAGATTAACCATTTTTATTTTATTTTGGTAAAATAGATTA ACCATTTTGTTAGAAAAATGAAGTTTAAAACATTTACAGTTCTACGTGTACATGCTTCGACCAATTTTCTTAACATGTAA CTTCCACTCTAAGAAACTTCAAAACCAAGCAAAAAAAAACTCACGAAAAGCAACTCTTCTCCTACCACCAAATTCCGAAA ACTTAATTCCTCCTAAAACGAACACGACACTACTCTATACATATATGTTTGTATATAAAGTTATACATACAATGGTCACA TTGATAGACGAAGAAAAAGACAAAAAAAAATCAACCATGGTTTCGAAACCGCTATTCTCCCTATTGCTTCTCACGGTTGC ATTGGTTGTGTTTCAAACCGGAACCCTTGTCAACGCTGAAGATTCCGAGCCTTCGAGTTCAACGAGAAAGCCTCTGGTGA AGATTGTGAAAGGTAAAAAATTATGCGACAAAGGATGGGAATGCAAAGGATGGTCCGAGTATTGTTGCAACCATACAATC TCCGATTTTTTTGAGACATATCAATTCGAGAATCTATTCTCTAAGCGTAACAGTCCAGTGGCTCACGCCGTCGGCTTTTG GGATTACCGATCATTCATTACTGCGGCTGCCGAGTACCAGCCTCTTGGTTTCGGTACTGCTGGTGAGAAACTTCAAGGGA TGAAGGAAGTCGCTGCGTTTCTTGGCCACGTTGGGAGCAAAACCTCATGCGGGTACGGGGTTGCAACAGGCGGACCATTA GCTTGGGGATTGTGTTACAACAAGGAGATGAGTCCTGATCAGCTTTATTGCGATGATTACTACAAATTGACATATCCTTG TACTCCTGGTGTCTCGTACCACGGTCGAGGAGCTTTACCGGTTTACTGGAATTACAATTATGGTCAAACCGGGGAGGCTT TGAAAGTGGATCTACTGAGTCATCCAGAGTATCTCGAGAACAATGCTACGTTAGCGTTTCAAGCGGCGATTTGGCGGTGG ATGACGCCACCGAAGAAGCATTTACCGTCGGCGCACGATGTTTTCGTCGGAAAATGGAAGCCAACAAAGAATGACACTGC TGCAAAACGAACTCCGGGGTTTGGAGCCACCATAAACGTTCTTTACGGCGATCAAATCTGCAACAGCGGATTCGATAACG ATGAGATGAACAACATTGTTTCTCATTATTTGTATTATCTTGATCTGATAGGTGTTGGACGAGAAGAAGCTGGTCCTCAC GAGAAGCTGTCATGCGCAGATCAAGAACCTTTTTCTTCTTCCTCCTCTGCTCCTCCGAGTTCTGGTTCCTCTTCTTGAGT GAAAGTTTTATAACCTCTGGTTCGGTGACTTTTGAAAAAGAAAGTTGACTTTGGTGTATATGTGTTTTGCATGGGCTTGG TTTTAATATTTTGTAAA ................................................................................ .................i.................................N......................N..... ................................................................................ ............................................N..........................N........ ....................................i........................................... ................................................................................ ......................N...........N.....N........N.............................. ................................................................................ ...............................................................................i ..............................................N................................. ...........................N........................N........................... ...........................................................N.................... ..........................................N..................................... i....................................N...............N................i......... ................................................................................ N....i.......................................................................... ...........N.................................................................... ...........................................................N..........N......... ................. Pos Score Pred ------------------------ 98 0.570 Yes 132 0.319 - 155 0.369 - 285 0.438 - 312 0.445 - 357 0.840 Yes 503 0.198 - 515 0.426 - 521 0.206 - 530 0.156 - 720 0.800 Yes 767 0.241 - 828 0.330 - 853 0.330 - 940 0.334 - 1003 0.276 - 1041 0.526 Yes 1078 0.266 - 1094 0.481 - 1111 0.568 Yes 1201 0.442 - 1206 0.578 Yes 1292 0.195 - 1420 0.083 - 1431 0.035 -

Explain the output -- Processing locus 571: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAAACCAAAAACCAGATTGTTTTGCGTTCATGGACATGTTCCTCTCTGGAGATTAGAATCGTAGCTATGACTATGTCTG AATTTCACAAGAGGTAGCTAACTCAGGCCTTTTTGCTCTTTTGACAGAAACACACTTCGGAAGATGGAGATCACTAACGT TACCGAGTATGATGCAATCGCAAAGCAGAAGCTGCCTAAGATGGTGTACGACTACTATGCATCTGGTGCAGAAGACCAAT GGACTCTTCAAGAGAACAGAAACGCTTTTGCAAGGATCCTCTTTCGGCCTCGGATTCTGATTGATGTGAGCAAGATTGAC ATGACAACCACCGTCTTGGGGTTCAAGATCTCGATGCCCATCATGGTTGCTCCAACTGCCATGCAAAAGATGGCTCACCC TGATGGGGAATATGCTACTGCTAGAGCTGCATCTGCAGCTGGAACTATCATGACACTATCTTCATGGGCTACTTCCAGCG TTGAAGAAGTTGCGTCTACAGGGCCAGGGATCCGATTCTTCCAGCTCTATGTATACAAGAACAGGAATGTGGTTGAGCAG CTCGTGAGAAGAGCTGAGAGGGCTGGGTTCAAAGCCATTGCTCTCACTGTAGACACCCCAAGGCTAGGCCGCAGAGAGTC TGATATCAAGAACAGATTCACTTTGCCTCCAAACCTGACATTGAAGAACTTTGAAGGACTTGACCTCGGAAAGATGGACG AGGCCAATGACTCTGGCTTGGCTTCATATGTTGCTGGTCAAATTGACCGTACCTTAAGCTGGAAGGATGTCCAGTGGCTC CAGACAATCACCAAGTTGCCCATTCTTGTCAAAGGTGTTCTTACAGGAGAGGATGCAAGGATAGCGATTCAAGCTGGTGC AGCCGGAATCATTGTATCAAACCATGGAGCTCGCCAGCTTGACTATGTCCCAGCAACCATCTCGGCCCTTGAAGAGGTTG TCAAAGCGACACAAGGACGAATTCCTGTCTTCTTGGATGGTGGTGTTCGACGTGGCACTGATGTCTTCAAAGCACTTGCA CTTGGAGCCTCCGGGATATTTATTGGAAGACCAGTGGTATTCTCATTGGCAGCTGAAGGAGAGGCTGGAGTTAGAAAGGT GCTTCAAATGCTACGTGATGAGTTCGAGCTGACCATGGCACTGAGTGGGTGTCGGTCCCTAAAGGAAATCTCCCGTAACC ACATTACCACCGAATGGGACACTCCACGTCCTTCAGCCAGGTTATAGAGAGGAAAATAACCAACAAGCAGAGAACAGCAG CAACACAGAACCAAAATATTGGCTAAACTTATTCATACTCTGATTGTACTTGGCTTCTACTCTGCT ..............................i.....N..............................N.....N...... ...............................................................i................ ........N..N............................i...............N.....................N. ...............................................................N................ i................................i........i.................N........i.......... ..N........N.....................................N.............N................ ................................................N.................N............. ................................................................................ .........................................................................i...... ......N....................N......................................i............. ....................................................N........................... .......................N....................N................................... ....................................N.......................N................... ................................................................................ .......N.........i................N............................................. .............N.................................................................. .................................................................. Pos Score Pred ------------------------ 31 0.546 Yes 37 0.351 - 68 0.426 - 74 0.356 - 144 0.774 Yes 169 0.304 - 172 0.291 - 201 0.563 Yes 217 0.190 - 239 0.454 - 304 0.182 - 321 0.758 Yes 354 0.666 Yes 363 0.762 Yes 381 0.350 - 390 0.791 Yes 403 0.254 - 412 0.460 - 450 0.373 - 464 0.492 - 529 0.376 - 547 0.290 - 714 0.771 Yes 727 0.247 - 748 0.336 - 787 0.516 Yes 853 0.281 - 904 0.210 - 925 0.379 - 997 0.330 - 1021 0.206 - 1128 0.377 - 1138 0.532 Yes 1155 0.499 - 1214 0.489 -

Explain the output -- Processing locus 572: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCCTTCATTGAAGTAGCGTAACATTGAAGTAAGCGGTAACATTTCGGAAGAATCTCTTTCTTTAGGTGAGTTTTCGTTA ATCTTCGGCGAGTTTGGTTAAAGTAATGGTTTTTGTTCTGTCTTTGCATTCTTGAGATCTATTGATTCGGATTTTGGATT CAGAACAATGTTATTGGTTTGCTCATTGTTTCAGATTACTAACTTTGTTTCTTCTTTCTGTGTGTTTTCAGTTGATATGA GCCAACAACGTACACGCTCTCGGAACCCGTTCAAGATTCTGTTTACGAAGAAGAACAATGCTCGATTATCGCCAAAGCTT CTCTCACTGTTGAACAATTTCGAGACTAACTTGACGGTATCGATTAGAGAACTTGTACCAAAGGATAAGAATGATATCGT TAGTGTTTCTTGGATGATACAAGCCATGCAGTCTCTTTGTGAGACACACAAGAGTATCAGGACGCTCGTGAAGGATTTAG AGGTTCCAGTATCGGATTTGGAGGAGAATTTCATTTATATCTTCTCTGACTTTAGCTTGAAGCGGGAACTCTGCAACGCC TTTACCTCAGAGATATATCATTTGGAACATGGCAATCTATTACTAAAGTTTGCTTTTAGTAAGCTGGAGACAAACAGTGG CAATATTCTTCAGCGTTGGAATCAGCACATGGTTTCCAAGAACCCGAGTATCGAAAACTGGGGCGCGGTTTTGAGTAGGC TGGTTGAATCGATGGATCATCATCATCATATCTCCAAGAAGGGGAAGAAGAAATACTCTGCGGAAGGAAAAGTTCTCTTA CGAGTTTTATACGGTGTAAAGGTCAAGACTTTATACATATTCAGCGTGTTTGATGCATCGTTCTCGGGTTCTTCCAAGAA TCTCTTCTATCTAACCATTCCAAAGGAGATGGAGGAGCTTCCATGGGTTCAAGCTTTCATGGAACTGCAGAACACTATTA ACCCGGAGATCAAAAACACATTTTTATCAGACGGATTCACGGTTATAAAAGATCTCGAGGCTGTTGAAACCGGTGTGAAG AAGCTTTACACAGCGGTACAAGAAGGGTCGGTTCCTATTTTGTTAGTGGAACCATTGGAGAAGTCAGTGATAGAACTTTC GGAAAGGTTTGAGCTAGTCTCCAAGGAAACACGTTGCTTGTCGAAGATGGTGATCTCTGCCCGAGACGCATTGTTGGAGA GCCTGTGGACGAAATGCGCAGAAGAGCTAGGAGTGACATTGCCAACGATGGTTAAAAGTGTTAATATAGAGGAAAAGAGC TAAGACTTGTTTGTGAATATAGAGGAAACATGTAATTATGGTATCGTTGATGTTAAGTAATAACAAATGCAGATAAAAAT GAGACTTTAAAACGCTTCAAAT ................................................................................ .........................N...................................................... .......i....................................................................N... .........................................................i...................... ......................................................................N......... .............N...........N...................................................... ................................................................................ ............................N................................................... ............................i................................................... ...........i.................................................................... ....................................................N........................... ............................i.............N...............i..................... ................................................................................ ................................................................................ ..............................................N................................. .............i.................................N................................ .............................N.......N...........N................N...........N. ...................... Pos Score Pred ------------------------ 106 0.184 - 168 0.753 Yes 237 0.406 - 298 0.710 Yes 391 0.355 - 414 0.101 - 426 0.332 - 589 0.181 - 669 0.602 Yes 732 0.505 Yes 853 0.169 - 909 0.613 Yes 923 0.489 - 939 0.607 Yes 1167 0.296 - 1214 0.513 Yes 1248 0.460 - 1310 0.082 - 1318 0.081 - 1330 0.038 - 1347 0.049 - 1359 0.070 -

Explain the output -- Processing locus 573: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTAATTGGAATAGTGGAAGGGGTTAAAAGAAGCAACTAGAGAGTGGAGCCACTCGTTGCTCTCTTAAGAGGAAAGAAAA AAGCCAGTGGTTACGCAATGAAGAACGTATGCTTTTGCTTCTAAGCCTTTGGTTTTTTATGTGTGGTTCTCTTTTCTTAT CTCTATGAACCAACACCAAACATTTTCCAACATTCCTTCATTATATATCAGAACAAAACATTATTCGTTCATGAGAGAAA CAATGTCTGAAATAGAAAAGCAAAAGCCGGAAGAAGAAGAATCATCCACGACATGGCCGGATATCGAAGAAGAACTCGTG CTTCCCGATGTGTTACGTAGTGAAGGCGTGAAGAAACTGCATATGAGTATTCAAAGTGAGTGGGATTACCTTCAGAAGTC TGCTTGTCAAACAGCTGCAGGAAGAGCTTTGTGGAAACATGTTATTCATGATCCGCTTGCTCATTTGTTTGCTGGAGAGA CACATTTGAGAAACCTACACACAAAGATTCAGACTGACCGTCTCAACAATGCTCGAGAGGTTTCTGGTGTGATCCTAGCC GTTCGAACTCTCTGGTTTGACACAAGGATTCAAGCTGCTCTGGAGTCTTTCCACGGCGATGCAGCACAGGTTGTTCTACT TGGTGCAGGAATGGATGCAAGATCATACAGACTAAACTGCTTGAACAAAAGTGACGTATTCGAAGTGGATTTTCAAGATG TTTTGGAAACCAAAGCGAGCCTAGTTCAAGCTGCAGTAAACTCTAGAGATGAGCTTAGGATGACAGCGAAATCACTAGTC AGAGTAGCAATTGATATCAGAGACAACGATTGGTTTGAACAGCTTAAAAAATCAGGTTTTTTGCCAGAGATTAACACGGT ATGGGTTCTCGAAGGTATTCTCTATTACCTGTCTCACACAGAGGCAATGCAAGTACTAAACCTCATAGCTGAGAAGTGTG GACTAACCAGCACAGTTCTCTTGGCGGATTTCATGAACAAACCATCAGCTACACTTCCAAACTCTGTGTTCCACTTTTAC AGTGACTGGCCTGATCAGCTCTTACCAACTTTGGGATTCTCTCATGTCAAGCTTTCACAAATAGGTGATCCAGATGCGAA TTTCGGTCTGCTCCACGATCCACGGAATCTCTTCAACAAACTTCTCCGCCTGCCAAGGACTGCACAAATCCATCCAGATG ATGGAAAACCATGCTGCCGTTTGTATTTGGTCGAAGCTTCCGGTTCACCGCCTCAAGATAACCAAGCGAGCTTGTAAGCT CCTTCCGGACGTGCATTTGGCACTGCACGCTTTTGCAGAACAGATATATATGTGAGTCTTTCACACTAGAGAAATTGTGT AGGAGGTTAATGTAAT ................................................................................ .................N..........N.............................N..................... ....N.................................................................N......... ..i.................................................N........................... .......N..................................i..................................... ......................................N........N................................ ................................................N............................... ..........................................................N..................... ..........i...N..............................................................N.. ................................................N..........N.................... ................................................................................ N.............................................i................................. ................................N............................................... ...........................................N.............................i...... .............................................................................i.. i.........N..................................................................... .................................................N.............................. .........N...... Pos Score Pred ------------------------ 98 0.156 - 109 0.083 - 139 0.378 - 165 0.097 - 231 0.404 - 243 0.714 Yes 293 0.441 - 328 0.357 - 363 0.671 Yes 439 0.394 - 448 0.094 - 529 0.446 - 619 0.191 - 651 0.659 Yes 655 0.253 - 718 0.275 - 769 0.144 - 780 0.453 - 881 0.369 - 927 0.667 Yes 993 0.375 - 1084 0.391 - 1114 0.671 Yes 1198 0.702 Yes 1201 0.624 Yes 1211 0.490 - 1330 0.071 - 1370 0.034 -

Explain the output -- Processing locus 574: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAAGCTTCAGGGTTTAGCTGTTTTGAAGTTGCGTTCTCTAAACGTCGCGTTTCAGGGTTTTAAAACGCTACCGCACTTT CCGAATCGCTGAATCATATAAGGCTCAATGTCTTTCTTTTTTTTTTTTTTTTTTTGGCCTAACTCTGGCTTTGAGAGAGA CAGAGAGGTAGAGAGAGTAACGATGGCTGCTGTAGAAGAAGTAGACGATGTTGGAAGAACAATAGAGGAAGAGACTGTGT GTGAAAAAAGAGAAAGAGGAGAGGAAGCTGAAACATTGACTCCATGGGAGCAACACTCCAGCATCATAAGCATCCCTCGA TTCGACTACAAAGCTCCCTCTTCTCTGCTTCATCATTCTCATTCCGGATTCCTCGTCACCTGCAACATCAAAAGGGAGAA AAGTGCTACTAAAGAGGTCATGTCCATCCTTGGAAAGTATATTGGGTCCATGCATGAGGAGAAACCTGAAGTTCTAAACT CTACTGCATCCAAAAAACAAAAAGTTTGTGCACAAGAGACAGAGGAAGGCGGAGAGAAGACTGTACCTCTTGAGAATGAT GCCTTGCAGGAAACAGGTGAAAATCCAAATGTTGAGGATCTTAAATTAGCAAATGAAGAACACAATTCTCTCATGTCATT GGTTAAGCTAACAAAGAGTGGCTTGCTTCTCTTTACTTTCCCTGTTGAAAACTCTCCCAATACAACGAATATCGTATCAC GAGTCTTCCAGTCTATGGAATCTGGAGCTTTGAAGGCACCAATCTGGTGCCATCGGATATTCCCAGTTCAAGCTACTTGT GGTTTGACAGAGAAAGAACTTCGGGAAACAGTATCAAAGCTTGTTCAGCGGTTTGTCAATGATAAAGACAATACACTATC AAAACCTGTGAAGTTTGCAGCTGGGTATCAACGTAGAGGTGCGGAGGAGACGAAAGGAAAGATTCGGAAAGACGCTTCAG ATGTTCTAGTTCAATGTCCGTTGCTGGACCGCATTAAATGCTTTGAAACTGTGGCAGCTGGAGTGAAAGACATTGTACCA GATTCAGTTGTGGATCTCAAATCTCCAGAGTTATGTGTTCTGGTGGAACTGTTACCGCTCTCTAGAATATCAAGTGGCTC CTTTGTAGCTGCTGTTTCAGTGCTTCCACATAGACTCGTTAGCACAAAGCCGAAACTCTGCATCAAGCCTCTAGTCCCTG AATCCAAACACAAAAAGGGACCAAACTGACAGGTGCAAAGGCTGATCGCTGCATTCGATTTTCGAGAGCCAAAGATATCA ATAGAAGTTGTAATCTCTCTGTTTTTGTTTAGATATGTCTTTTGCTTT ................................................................................ ...........................i.................................................... ......................i........................i................................ ...........................................N.................................... ................................................................................ ...................i.............................i...i.......................... ...........................................................................N..N. ............................N.......................N...................i....... ................................................................................ ..............N................................................................. ..........................................................N..................... ................................................................................ N............N.......................i.......................................... ................................i............................................... ................................................................................ ................................................................................ ..................................N............. Pos Score Pred ------------------------ 108 0.686 Yes 183 0.909 Yes 208 0.508 Yes 284 0.147 - 420 0.704 Yes 450 0.599 Yes 454 0.518 Yes 556 0.117 - 559 0.168 - 589 0.223 - 613 0.199 - 633 0.681 Yes 735 0.470 - 859 0.213 - 961 0.182 - 974 0.352 - 998 0.583 Yes 1073 0.658 Yes 1315 0.051 -

Explain the output -- Processing locus 575: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAGTCTCTTGGCAATATGTAAAGAGGCGAAATCAAAGTTGATCAACAATTAAACACAAAAACCCTTAAGTTGACGTGGC TGTATCTCTTCCAACTCTTAAAAATCAATTACTATTATACCCCTCAGAAAAATGGCAGAGCGTGTGTCTCTCACACTCAA CGGAACCCTCCTTTCTCCTCCTCCCACAACAACAACAACAACAATGTCTTCTTCTCTCCGATCTACCACCGCCGCTTCTC TTCTCCTCCGCTCCTCCTCCTCCTCCTCCAGATCCACTCTCACTCTCTCCGCTTCCTCCTCTCTCTCCTTCGTCAGATCT CTCGTCTCTTCTCCACGTCTCTCTTCTTCTTCATCCCTCTCTCAGAAATATCGAATTGCGTCGGTGAATCGGAGTTTCAA CTCCACCACCGCCGCTACTAAATCTTCATCTTCAGATCCTGATCAGTTGAAGAACGCTAGAGAAGATATCAAAGAGCTTC TCAGCACCAAATTCTGCCATCCGATTTTGGTTAGATTAGGTTGGCATGATGCTGGTACGTATAACAAGAACATTAAGGAA TGGCCACAAAGAGGTGGAGCTAATGGAAGTTTGAGATTCGATATTGAGCTTAAACATGCTGCTAACGCTGGTCTTGTGAA TGCTTTAAACTTGATTAAGGATATCAAAGAGAAATACTCTGGGATCAGTTATGCTGATTTGTTCCAGTTAGCTAGTGCTA CTGCTATAGAGGAAGCTGGAGGACCGAAAATACCGATGAAATATGGTAGAGTTGATGCCTCTGGTCCTGAGGATTGTCCA GAAGAAGGAAGGCTTCCAGATGCTGGTCCTCCTTCACCTGCTACTCATCTCAGAGAAGTTTTTTACAGAATGGGATTAGA TGACAAGGACATAGTTGCATTATCTGGTGCTCACACGTTAGGAAGATCTAGGCCAGAACGTAGTGGTTGGGGGAAGCCTG AGACGAAGTACACGAAAGAAGGACCTGGAGCACCAGGAGGACAGTCATGGACACCAGAGTGGCTGAAGTTTGATAATTCT TACTTCAAGGAGATCAAGGAAAAGAGAGATGAAGATCTCCTTGTCCTACCCACTGATGCTGCCATCTTCGAAGATTCTTC TTTCAAGGTCTATGCTGAAAAGTATGCTGCAGATCAGGATGCATTTTTCAAGGATTACGCTGTAGCCCATGCGAAGCTCA GCAATCTTGGAGCAGAATTTAACCCTCCGGAGGGTATCGTTATCTAATGGGGGCACATAATTTACTCAACCAGTAAGCTT TCTTCTTTGTATTTTGTTTTTTTTCCTCATTCGAAATTCCAAATGTACATGATGTTTAACTTCTTT ................N............................................................... ...................................................i............................ ...........................................i.................................... ................................................................................ ................................................................................ ................................................................................ .............................................N..N..............................N ......................N................................N.......................N ..................................................N............................. ...................................N......N...........N......................... ...................N.................................................i.........i ................................................................................ ..............................................N................................. ............................N..........................N........................ ...........N...........N..............N.............................N........... ..............................................N................................. ..........................................N.....N..N.............. Pos Score Pred ------------------------ 17 0.330 - 132 0.808 Yes 204 0.718 Yes 526 0.336 - 529 0.168 - 560 0.415 - 583 0.393 - 616 0.098 - 640 0.058 - 691 0.117 - 756 0.347 - 763 0.405 - 775 0.421 - 820 0.303 - 870 0.569 Yes 880 0.666 Yes 1007 0.163 - 1069 0.327 - 1096 0.278 - 1132 0.107 - 1144 0.116 - 1159 0.446 - 1189 0.180 - 1247 0.287 - 1323 0.070 - 1329 0.037 - 1332 0.071 -

Explain the output -- Processing locus 576: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 577: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGAGTTAGATAACTTTTAGACAAAAAAAAAAAAAAAAAAAGGAGTTAGATAACAAAGTAACCAGAGAGATTGATCAGAGC AAATAAATTTCGGGAGTTTGAGATTATCATCTTTCAATTGAGAATAGTGAAACGAAGAACATACAGATCACGCGAGAGAG AGTGATCAGAGACAATCAATCGCAAAAGATGTCGCCGGAGATGGAATTCGTGAAGGTTTTGGGAAAGGGTACTTACGGCT CCGTCGAGCTCTTCAGCCACAAACAAAACGACGGATCGTTGCTCTACAACGCAGTGAAGATCATGGACTCTGAGAACTAC GGTTCTATAGACCAAGAGTTTCGAATTCTGTCGGAACTCAGAGGATGTCCTTGTATCGTGCAATTGTGTGGGAACTCTCT TGTTCAAGGAATTGATTGCAATGGAAAAAAAGTTTACATGATGTCAATGGAGTATGCAGCTGCTGGTACTCTAACTAATT TCATCAAAAGAAACCGAACGAAGTTGTCGGATTCTGTTATTAAAGACTTCACTCGTATGATTCTACAAGGATTGGTCTCG ATTCATAATCATGGTTATGTTCATTGTGATCTTAAACCGGACAATATCCTTCTTTTTCCTCTTTACGATAAAGATACGTG GAATTGTTCTTACGAGTTGAAGATTTCGGATTTCGGAATATCGACAAGGGCCGGAGACAAATCTGGTTGTTGGAGAGTCG ATGAACCGTGGGTGGGAACGTCTATCTACATGTCTCCTGAGTCAGTCAGCGACGGCACCACCGTTGAGAAAACCCTAGAT TTGTGGTCACTTGGTTGCATTGTGTTGAAGATGTATACCGGTAAGCGGCCATGGTTAGGGTTTGAGAAAGATGTTAAGTC TCTTCTTTTGAATCAAAAGGCACCAGAGATTCCAGAGACTCTGCCGTGTGATGCAAGGCTGTTTTTGGAGAAGTGTTTCT CAAGAAAACCTGAGGAGAGAGGATCAGCTTCGGAGTTGCTGTTGCATCCGTTTTTGACCGGAGATGAGAAGAAGGGTTCT TCCGTCGCCGGTGGAGAGAGGACGGGGATGGTGTTGAGGCTCAGAAAACCTCCGCCGATATCGAAAGATATTCCAACGAA GCCACGGAAATTGAAGGTTATTTCACAAAAGCCCCAACAGTTAAAGAAAGTCTCGAATAAACCCCTAAAGGTGAAGATTG TTCCTCCGAGACCCCCAAGATCCGATTTTGTCCCCGTTCTATAAACTACTGAAGTTAAACCATGTTACTACTCTAGTATA AAAAATGTAGATTTTGCAAATTGCCAAATTATATTGCATATGAAATTTATTGATGTCTTTTTT ................................................................................ ................................................................................ ............................i...........N....................................... ..............................................................N................. ............................................N................................... ....................N................i..i.....i......N.......................... ........................................................N....................... ..........N.....N............................................................... ................................................................................ N............................N.................................................. ..............................N...................i...................N......... ..................................................N............................. ...............................................................N................ ...........................N.................................................... ................................................................................ .............................................................N.................. ....N..................................N............N.......... Pos Score Pred ------------------------ 189 0.694 Yes 201 0.435 - 303 0.130 - 365 0.430 - 421 0.083 - 438 0.626 Yes 441 0.577 Yes 447 0.717 Yes 454 0.166 - 537 0.382 - 571 0.300 - 577 0.160 - 721 0.085 - 750 0.462 - 831 0.129 - 851 0.580 Yes 871 0.232 - 931 0.411 - 1024 0.418 - 1068 0.359 - 1262 0.141 - 1285 0.039 - 1320 0.062 - 1333 0.059 -

Explain the output -- Processing locus 578: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCGTCTCTCAGACTTCAATCAAAGATTAAACACATGGACCAGAAATAAGACTAAATGGGCCTTAATAATGAATATCAACA AAGCCCAATAAGGGCCTTTAAGTTGTCTCCGTGCAAAATACGACGTATATGTGTCTCCACCACTCACTGTTAACGATCAG TTTCAGCGAAACGATGGAGCTTCTCTGCTCACCAACGTCTCTTTCCTCCTCCTTCGCTCTCTCTTCTGCTCTTTTAGTCC CTCGTTCGTTCTCCATGCCAGGGACAAGGAGATTCATGGTTCTGTGTAGTTCCCAGAAGGAAAGTCAGATGACAGTGTCA GTAACTGGAGCAACTGGATTCATAGGGAGACGATTGGTTCAGAGACTTCGTGCTGATAACCACGCTATCCGGGTCTTAAC ACGCTCTAAATCCAAAGCTGAGCAAATATTTCCTGCCAAGGACTTCCCAGGTATTGTGATTGCAGAAGAATCGGAGTGGA AGAACTGTGTACAAGGCTCTACTGCTGTTGTGAACTTGGCAGGACTGCCTATTAGTACAAGGTGGTCTCCCGAGATCAAG AAAGAGATTAAGGGCAGCCGGATCCGTGTCACCTCCAAAGTTGTTGACTTAATCAATAATTCACCAGCAGAAGCTAGGCC TACCGTTCTAGTTAGCGCCACGGCTGTTGGTTACTATGGTACTAGTGAGACAGGAGTATTCGACGAAAATAGTCCATCAG GGAAGGACTATCTTGCAGAGGTTTGCAGAGAATGGGAAGGAACTGCATTGAAAGCAAATAAGGATGTTAGAGTGGCACTT ATCCGCATTGGTGTTGTTCTTGGCAAAGACGGTGGAGCTTTGGCCATGATGATACCCTTTTTCCAAATGTTTGCTGGAGG ACCTCTGGGTTCCGGACAACAATGGTTCTCTTGGATTCACGTGGATGACTTGGTGAACTTAATTTACGAGGCCCTCACTA ATCCATCTTATAAAGGAGTTATTAATGGGACTGCGCCGAATCCGGTTAGGCTTGGGGAAATGTGCCAGCAGCTAGGCAGT GTTCTTAGCCGACCATCGTGGCTACCAGTTCCTGACTTTGCACTCAAAGCTTTGCTCGGAGAAGGTGCCACTGTAGTTCT GGAGGGACAGAAGGTATTACCTGTAAGAGCCAAAGAGCTTGGTTTTGAATTCAAGTATAAATACGTGAAGGATGCACTCA GAGCCATTATGCAATAAGGTGACACACATAAGCATCTTTGTAAAATTGGTTTTGATCACAGAAAATCCCCAGAAAAACAA GATATTCTTTTCTTTTCGTCTCTATATCTTTCTTTT .................................N....................i............i............ ................................................N............................... .............i.................................................................. ..............N....................N................................i........... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ...................................i............................................ ...............................N...............................N................ .............................................N..N.................N............. .....................i......................N................................... ........................i..................................i.................... ................................................................................ .......................................................................N........ ........N....................................................................... .................................... Pos Score Pred ------------------------ 34 0.415 - 55 0.570 Yes 68 0.741 Yes 129 0.452 - 174 0.676 Yes 255 0.443 - 276 0.212 - 309 0.710 Yes 676 0.565 Yes 752 0.168 - 784 0.172 - 846 0.424 - 849 0.198 - 867 0.427 - 902 0.524 Yes 925 0.317 - 985 0.734 Yes 1020 0.603 Yes 1192 0.301 - 1209 0.168 -

Explain the output -- Processing locus 579: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGATTAGCTTTCACTTACTTCCACTGAAGAAATAACTTTTGGAACATTCAACAAGACAACAAAATATGACTTCCCCATCA TCCACCTTCAGACCAAATTAAGTTCTTCAATCTTGTTTCCCTGTTTCACACACATATATATATATATATATATATATATA TATATATGTGTGTGTTTGTGTGCAGACGATGATGTTCTTACCAACCGATTATTGTTGCAGGTTAAGCGATCAAGAGTATA TGGAGCTTGTGTTTGAGAATGGCCAGATTCTTGCAAAGGGCCAAAGATCCAACGTTTCTCTGCATAATCAACGTACCAAA TCGATCATGGATTTGTATGAGGCAGAGTATAACGAGGATTTCATGAAGAGTATCATCCATGGTGGTGGTGGTGCCATCAC AAATCTCGGGGACACGCAGGTTGTTCCACAAAGTCATGTTGCTGCTGCCCATGAAACAAACATGTTGGAAAGCAATAAAC ATGTTGACGATTCTGAGACTTTGAAAGCTTCTTCATCAAAGAGGATGATGGTTGATTATCATAACCGAAAGAAGATCAAG TTTATACCTCCTGATGAGCAATCCGTGGTTGCTGATAGGTCGTTCAAATTGGGCTTTGACACTTCCTCCGTAGGTTTCAC TGAAGACAGTGAAGGATCGATGTATCTAAGCAGTAGTCTAGATGACGAGTCAGATGATGCGAGGCCACAAGTTCCTGCAA GAACAAGAAAAGCTTTGGTCAAAAGAAAACGAAATGCAGAAGCGTATAATTCACCTGAGAGAAATCAAAGAAATGATATC AACAAGAAAATGCGTACTTTGCAAAATCTACTACCTAACTCTCACAAGGACGACAACGAATCGATGTTGGATGAAGCAAT CAATTATATGACAAACCTTCAACTTCAAGTTCAGATGATGACGATGGGTAACAGATTTGTTACACCATCAATGATGATGC CTTTGGGGCCGAACTACTCTCAGATGGGTCTAGCAATGGGTGTGGGAATGCAAATGGGCGAACAACAGTTTCTGCCTGCA CATGTTCTAGGAGCTGGCTTGCCTGGGATTAATGATTCAGCAGATATGCTAAGGTTTCTTAACCATCCTGGACTAATGCC AATGCAAAACTCTGCACCTTTCATTCCAACGGAAAATTGTTCCCCACAATCTGTCCCTCCATCGTGCGCTGCTTTCCCTA ACCAAATACCAAATCCCAACTCTTTGTCAAATTTAGATGGTGCAACCTTACACAAGAAATCAAGGAAAACTAACAGATGA AGGAATTCTCCCTGGTAAAAATCTGGAAACCCGTGAGCAGATGAAGTAAACAGTGATGATTGTTACCTGCGGATTCACGG TCTTCTTCTTCATGAGTTA .................................................................N.............. ................................................................................ .....i......................i..N...............................................i ..................N............................................................. ......i.........N.........................i...............N..................... ...................................N..............N..........i.................. N...........................................i..i................................ .............N.................................................................. ...................i.....................i...........N..N....................... .................................N......................................N....... .........i.....................................................N......N......... .......N..........................N..i.....i..........................N..N..i... .......................i...........i...........i.....i.......................... ....................................N.......................................N... ........................................N..............N........................ ...........N....... Pos Score Pred ------------------------ 66 0.491 - 166 0.504 Yes 189 0.607 Yes 192 0.486 - 240 0.632 Yes 259 0.285 - 327 0.763 Yes 337 0.365 - 363 0.669 Yes 379 0.265 - 436 0.082 - 451 0.065 - 462 0.535 Yes 481 0.238 - 525 0.706 Yes 528 0.607 Yes 574 0.288 - 660 0.737 Yes 682 0.564 Yes 694 0.213 - 697 0.498 - 754 0.250 - 793 0.349 - 810 0.637 Yes 864 0.362 - 871 0.100 - 888 0.445 - 915 0.201 - 918 0.531 Yes 924 0.730 Yes 951 0.445 - 954 0.382 - 957 0.574 Yes 984 0.571 Yes 996 0.574 Yes 1008 0.504 Yes 1014 0.515 Yes 1042 0.162 - 1072 0.136 - 1086 0.504 Yes 1116 0.536 Yes 1122 0.648 Yes 1237 0.410 - 1277 0.167 - 1321 0.054 - 1336 0.046 - 1372 0.034 -

Explain the output -- Processing locus 580: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACGGATGGTCATGATTACTTGTGTAATCACAATCATGTTCTAATTTCTTGGGAACAAAAAAAAAAAAAAAAAAAGAAAT TGTGTAAAATGTTGTTGGTAATTCAAAGTTGATTACATTATACGGTCCAATATCAAACCGAACCGAACCGAACCAAGTTT GAGAGATGTAAAGTTGATCCAACGGATGAGATTGCATAATCGTGATCTCTCTATTCAAACCTTTTCCGTCGTTGATGACT GGCTGTTCCGTGAACTTCCCACTCATAGCAAAATGTTAGCTCAGTCGCACTTCTTCTCTAAGTCCTTCGATCTGATTCCG CCACAGTCTCCGGCGTTGCGGTCTGCAAATCCTTCTCTTCGTATCTCTTCTTCTTATAGTAACAGCCGCTTATCCTTTCT TTCTTCTTCTGCAATTGCTGTTCCCGTCTCCCGCCGTCGGTTTTGCCTGACTATGGCTTCAAACTCAAAACGGCCTAACA TAAGCAACGAGTCTCCTTCGGAACTTAGCGACACTGAGCTGGATCGATTCGCCGCTGTGGGAAATGCATTAGCTGACGCT TCCGGCGAGGTTATCAGAAAGTATTTCCGGAAGAAATTCGACATTGTTGATAAAGATGATATGAGCCCTGTTACAATCGC TGATCAAATGGCTGAAGAGGCAATGGTTTCCATTATATTCCAGAATCTCCCTTCTCATGCTATATATGGTGAGGAGAAGG GTTGGAGATGCAAAGAGGAATCTGCAGACTATGTTTGGGTTTTGGACCCAATCGACGGAACAAAGAGCTTTATTACAGGG AAACCCGTGTTTGGTACGCTGATTGCTCTGCTCTACAAAGGTAAACCGATTCTAGGACTGATTGATCAGCCTATTCTAAA AGAGAGATGGATAGGAATGAATGGAAGAAGAACTAAACTAAACGGGGAGGATATCTCAACGCGATCTTGCCCCAAATTAT CACAAGCATATTTATACACCACTAGCCCACATCTTTTCAGTGAAGAGGCAGAAAAGGCATATTCTCGAGTTAGAGACAAG GTGAAAGTGCCCTTATACGGCTGTGATTGTTATGCTTACGCTCTCCTGGCTTCTGGATTTGTCGATCTTGTTATTGAATC CGGTCTAAAGCCATATGATTTCCTTGCTCTGGTGCCTGTAATAGAAGGCGCTGGAGGTACCATAACCGATTGGACTGGTA AACGATTTCTGTGGGAAGCTTCATCCAGTGCAGTTGCGACAAGCTTTAATGTAGTTGCAGCAGGAGATTCAGACATTCAT CAGCAGGCTTTGGAGTCACTTGAGTGGCATTGAAAAGATTCGCTGGTATGTTCTCGCCTACTTATTGTTTCTACACACTC CCTTGCTTGAAGACATAATTATGATCACTTTCTTCATACTTGCTTGTTTGAT .....N.....N.......................N............................................ ........i....................................................................... .....N...................i................................................N..... ................................N............................................... ................................................................................ ....................................................i........................... ...............................................................N................ .......................................................N....i................... .......i..............N.................................N........N.............. .......i......................N................................................. ................................................................................ ......i.........i...i........................................................... ................................................................................ ...............................N................................................ ..............N................................................................. ................................................N............................... ...............................................N................................ ....................N............................... Pos Score Pred ------------------------ 6 0.293 - 12 0.200 - 36 0.168 - 89 0.593 Yes 166 0.321 - 186 0.686 Yes 235 0.204 - 273 0.447 - 453 0.829 Yes 544 0.149 - 616 0.241 - 621 0.783 Yes 648 0.900 Yes 663 0.341 - 697 0.136 - 706 0.429 - 728 0.598 Yes 751 0.179 - 887 0.688 Yes 897 0.522 Yes 901 0.563 Yes 1072 0.283 - 1135 0.268 - 1249 0.263 - 1328 0.081 - 1381 0.083 -

Explain the output -- Processing locus 581: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACGAAAACTCAGTATCCTTTGAGCTTTTATGGAAGTAAATTCGAGAACGTGTTAGATATGTAGAGTTGGTTATGATTCTG GTCTACATTGTTATAAGTTAGATTTCTTCCTGCAGTGTGCTTGACCTTGCACTGGTTAATTATTATATCCATGGTATTCT CTACCACGGTCTGTATTTAGTTTTGATGATGATTTTTTTTTCTCCTGTTTTTATGCAGGTAAGTTCACGTATGGCCAGTA ATACTTTGAAAGAGATGAATAGTCTTCATGTAACTGAGAAGATAAGTGACTGTAAAGCTAGCTTGACCAAGCCATGTGTT GGTAAAATGAATGGAAAATCTGAAGATAGACCATTACCAAACTCTGCCTCCTTGGATTCCAGTGATTCTAAAGTTGTAGA AGCAGAGAAACCCGAGCCAGAGATAGCTATTGTGGAAGTTGAATATATTGAATCTGAAAACTTGGATAATGTAGACGATG CTGATGCAGTTCTGAAGTCTGTTTTAGCTGGACTAGAGTCCAAGGACTGGATTTCACTCTGTGATGCTCTTAATAACGTT CGCCGACTTTCGATATTCCACAAGGAAGAAATGATGCATATGCTTGAAAAAGTGATACCACTTGTCGTGAAATCACTGAA AAATCCAAGAAGTGCGGTGTGTAAAACGGCATGCATGACATCTGCAGATATCTTCAGTGCATATAATAATCATATAACAG ATCTGTTGGAGCCTCTGCTTACTCAACTACTCCTCAAGTCTTCCCAAGACAAACGGTTTGTTTGCGAGGCAGCAGAGAAA GCTTTAACAGCAATGACCAAATATGTTTCCCCAACCTTGCTGTTACCAAAGCTGCAACCTTGTCTCAAAAACAGGAATCC TCGGATCCGTGCCAAAGCATCATTATGCTTTTCTCGAAGTGTTCCTCGACTGGGCGTTGAAGGGATTAAAGAATATGGAA TCGACAAATTAGTTCAAGCAGCCGCATCTCAGCTTAGCGATCAGCTCCCTGAATCCCGTGAGGCTGCACGGACTGTCCTA CTAGAACTTCAGAGCGTATATGAAAAAGCTCATCCTCTCATCAACCCTGAGACATCATCACCAGAAGAACAGCAAATCCC GGAGGTAGAGCCAATTACTTGGGAAACTTTCTGCAAGTCAAAACTGTCGGCTCTTAGCGCACAAGCCGTGCTTCGTGTCA CCAATGTTGTCACTGTTACTGCTCGGGAGGGTTTGGTTACTACAGGTTCATCATCTTCATCCCAGTTATAAACATTTTCT ACTACAAATTTTTTAAAAACCGTTTGCTACGAGTCATACGGTTTACATGGTATTCTCCGGCATGGCGGCATGATCTACCA AATTGGCAAA ............................N............................N.............N........ ......................................................................N......... .........................N..i.......................i.................i......... ..............i............N.............................................N...... ......i...N..................................................................... ....................................................................N........N.. ...N...........................................................N................ ..............................N..i.....i........................................ ..............................i...N............................................. ................................................................................ ............i.........N......................................................... ........................N.................................................i..... ................................................................................ ...................N............................................................ ................................................................................ ...N............................................................................ ..............................................N..............N.......N.......... .......... Pos Score Pred ------------------------ 29 0.296 - 58 0.420 - 72 0.353 - 151 0.087 - 186 0.436 - 189 0.650 Yes 213 0.685 Yes 231 0.588 Yes 255 0.538 Yes 268 0.201 - 314 0.246 - 327 0.713 Yes 331 0.143 - 469 0.236 - 478 0.071 - 484 0.104 - 544 0.083 - 591 0.372 - 594 0.613 Yes 600 0.628 Yes 671 0.565 Yes 675 0.474 - 813 0.526 Yes 823 0.463 - 905 0.429 - 955 0.711 Yes 1060 0.277 - 1204 0.124 - 1327 0.075 - 1342 0.104 - 1350 0.063 -

Explain the output -- Processing locus 582: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGATTTCTCGAGAAAAAAAAAAGGAAGCAGAAGAAGATTCTTATTTTATCTTTACACTGTTCAGAAATCTTCGGTGGAC GGATAACTTGCAGAGTTCTGATTGAGGTTTCTTTTTCTTTTCCCTGTTTTCTCTTGGATCTTGTTTCTTCATGTGCGGAC TATACAAAAAACTGATTCGGTGAAATTAATCAGCTTTATGCTCTGTTTTGTTCGCTTTCAGGCTGGTTTTGTGAGAATTA TAGTTGCAGCAAGAAAGCGTTTCAGATATTTTTTAATGGCCGCTGAGGATCGAAGTGAGGAACTAAGCAGCAATGTAGAA AATGGAAGTTGCAATTCCAATGAAGGAATTAATCCTGAAACCAGCAGTCATTGGATTGAAAACGTTGTCAAGGTTAGGAA ACCGTACACAGTAACTAAGCAGAGAGAGAAGTGGAGTGAGGAAGAGCATGATAGGTTTCTTGAAGCTATCAAGCTTTATG GTCGTGGTTGGCGTCAAATCCAAGAACACATAGGTACAAAAACCGCTGTACAGATACGAAGCCATGCTCAAAAGTTCTTC TCCAAGATGGCTCAGGAAGCTGACAGTAGAAGTGAAGGATCGGTTAAAGCGATTGTGATCCCGCCTCCTCGTCCAAAGAG AAAACCGGCACATCCTTATCCTCGGAAATCGCCTGTTCCATATACTCAGTCTCCTCCACCAAATTTGTCAGCTATGGAGA AAGGAACCAAGTCTCCAACCTCAGTGTTATCATCGTTTGGTTCAGAGGATCAAGTCAATAGATGCTCTTCGCCTAATTCG TGTACCAGTGACATCCAATCCATTGGTGCAACTTCCATTGATAAAAAGAATAACTACACAACATCCAAGCAACCTTTCAA AGATGATTCTGACATTGGTTCAACACCCATTTCAAGCATTACTCTTTTCGGGAAGATTGTCCTTGTCGCGGAAGAATCTC ACAAACCATCCTCTTACAATGATGATGATCTTAAACAAATGACGTGTCAGGAGAATCACTACTCAGGGATGCTAGTTGAC ACTAATTTATCTCTTGGTGTATGGGAAACGTTTTGTACTGGTTCTAATGCATTTGGCTCGGTTACAGAAGCATCTGAGAA CTTGGAGAAAAGTGCAGAGCCGATAAGTTCTTCATGGAAACGGTTAAGCTCCTTAGAAAAACAAGGATCTTGTAATCCTG TAAATGCAAGTGGGTTCAGGCCATACAAGAGATGCCTATCAGAAAGAGAAGTAACATCATCATTGACGCTGGTAGCTTCA GATGAAAAGAAAAGCCAAAGAGCACGTATATGCTAGTTTTAGCCATTGTACAGTTTGGAGTCAATTTTGATGATCCGTGA ACATTGTTTTAGCCATTTGCTGGGTGACTTGGATCATAGCATTCCACGAACCAAA ................................................................................ ......................................................................N......... .....................................N.......................................... ...................................i....................................i....... ...............................................N.............................N.. ...............................................................N................ ......i......................................................................... .........................................................................i...... .............................................................N.................. ................................................................................ ..N............................................................................. ..................N..N..N.............i.............................i........... ....................N.........................N................................. .................................N.............................................. ...i...........................i................................................ ....................................................... Pos Score Pred ------------------------ 151 0.183 - 198 0.183 - 276 0.741 Yes 313 0.517 Yes 322 0.514 Yes 340 0.254 - 448 0.368 - 478 0.281 - 544 0.291 - 567 0.674 Yes 714 0.624 Yes 782 0.347 - 883 0.180 - 979 0.395 - 982 0.137 - 985 0.122 - 999 0.867 Yes 1029 0.574 Yes 1061 0.429 - 1087 0.262 - 1154 0.412 - 1204 0.525 Yes 1232 0.570 Yes 1282 0.175 - 1310 0.471 - 1350 0.127 -

Explain the output -- Processing locus 583: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCACTTTACACGCTTCTCAGCTTTTTTGGTCAATTTTGGCATATTTGTATTCAAAGATTGGGCATTTGCAATTGCTTGT ATCTGATGATCAATCTCATAAATGTGTTACAGACAAGAAACAATGTCTGGGCTGCTGGATGGAATCCCCGAAGCAGTGGC TCTGAGATGCCTGGCTCATGTGCCTTTACACCTTCATCCCAATTTAGAACTCGTCTCTCGCTCCTGGCGAGCTGCTATCC GCAGTCATGAGCTCTTCAGAGTCCGTAAAGAGCTCCGATCTTCTGAGCATCTCCTCTGCGTCTGTGCTTTCGATCCTGAG AACATCTGGCAAGTCTACAGTCCCAACTGTGATCGCTGGCTCACCCTCCCTCTCCTTCCTTCTCGTATCCGCCACCTTGC CCATTTCGGTGCTGTCACTACTGCCGGCATGCTCTTTGTCTTAGGCGGTGGCAGCGACGCTGTCAGCCCTGTTACCGGGG ATCACGACGGTACCTTTGCTACTGACCAGGTCTGGTCTTACGACTTTGTACAGAGACAGTGGACTCCTCGCGCTTCAATG CTCGTGCCTCGTGCTATGTTCGCCTGCTGCGTTCTTCAAGGGAAGATTGTTGTTGCTGGCGGATTCACCACTTGCAGGAA ATCCATATCTGGAGCGGAGATGTATGATCCTGAGAATGACGTCTGGACATCCATCCCAGATCTCCACCAGACTCATAACT CTGCCTGCTCGGGTCTAGTGGTAAACGGGAAAGTCCACGTCTTGCACAAAGGCTTGTCCACGGTGCAGGTTCTTGAGAGC GTTAAACTCGGGTGGGATGTCAAAGACTATGGCTGGCCGCAAGGTCCAATGGTGGTCGTTGAGGACGTGCTTTACGTGAT GAGCCATGGGCTGGTGTTCAAGCAAGAAGGTGACACGTGGAAGATGGTTGCATCGGCCTCAGAGTTTAAGCGGAGGATCG GAATGGCGATGACGAGTTTGAGTGATGAGGTTTTGATCGTAGGAGGAGTGATCGGACCTGATAGGCTCAATTGGGACATC AAGCCCTTGTCAGATGTCGACGCTTTGACAGTTGGGAATGATCGTCCGGCCTGGAGGAGTGTGGCGCCGATGACAAGGTG TCGTGGGACGATCCTTGGCTGCACGCAGTTGACAATCTGATGGATGGGAATGTGGCTTCATTATGACGTCTTTCAGTTCT TTTTTGTTTTGTATTTTCTTCCTGTTTGTTAACTGTTGTGAGCATTATTGTTTTTAAAT ................................................................................ .....N...............i....................i...............N..................... ......N..........N.............................................................. ......i......................................................................... ................................................................................ ............................i................................................... .............................................................................i.. ...............i................................................................ ...................i...N...........i............................................ ................................................................................ ................N...........N...................N.............................N. .....N.....................................i.................................... ..i.....i...............N....................................................... .............N.......................N...............................N.......... .......................................N...N.....N............N................. ........................................................... Pos Score Pred ------------------------ 86 0.327 - 102 0.578 Yes 123 0.721 Yes 139 0.273 - 167 0.489 - 178 0.383 - 247 0.705 Yes 429 0.667 Yes 558 0.882 Yes 576 0.561 Yes 660 0.747 Yes 664 0.221 - 676 0.654 Yes 817 0.277 - 829 0.285 - 849 0.450 - 879 0.383 - 886 0.319 - 924 0.696 Yes 963 0.528 Yes 969 0.548 Yes 985 0.187 - 1054 0.396 - 1078 0.249 - 1110 0.080 - 1160 0.309 - 1164 0.042 - 1170 0.142 - 1183 0.151 -

Explain the output -- Processing locus 584: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGGAATGTGAAAGTCAAAGAGAGAAAAAAACGCTCGAAAGAGAAAGAATCCAAAATGTGAAAAATCAGAACACATAAG CAGTGACGTCATACTTGATAAGTTGATATCATGAATTCGTGGCACACTGGAGATGACTCTATAAAAGCAGCGGCGTCATC GAGAGAAACCGAAGAAGAAGCAGTAACAAATTTGGTGAAGTCACGAGAATCAACGATGACGGCGACGAACAAGCAAGTCA TATTGAAAGACTACGTGAGTGGTTTCCCTACGGAATCCGATTTCGATTTCACTACCACCACCGTCGAACTTAGGGTTCCG GAAGGTACTAACTCTGTTCTAGTGAAGAATCTCTACTTGTCATGCGATCCTTACATGAGAATTCGCATGGGGAAACCTGA TCCTTCCACTGCTGCTCTTGCTCAAGCTTACACTCCCGGCCAGCCAATCCAAGGGTATGGAGTGTCTAGAATAATAGAAT CTGGACATCCAGATTACAAGAAAGGAGATTTACTCTGGGGTATAGTTGCATGGGAGGAGTACAGTGTTATCACTCCAATG ACTCACGCGCATTTCAAGATCCAACATACTGATGTTCCATTATCTTATTACACTGGACTTTTAGGTATGCCTGGTATGAC TGCCTATGCTGGGTTTTATGAAGTTTGTTCTCCAAAGGAAGGAGAGACAGTTTATGTGTCAGCTGCATCTGGTGCTGTTG GTCAGCTTGTGGGACAACTTGCTAAGATGATGGGCTGTTATGTTGTTGGAAGCGCTGGAAGTAAAGAGAAGGTTGATCTT CTGAAGACCAAGTTTGGGTTTGATGATGCATTTAACTACAAGGAAGAATCTGACCTTACTGCTGCCCTAAAAAGGTGTTT CCCTAATGGCATTGACATATACTTTGAGAATGTAGGAGGCAAAATGCTAGATGCAGTGCTTGTGAACATGAACATGCACG GGCGTATCGCTGTCTGTGGAATGATCTCACAGTACAATCTTGAGAACCAGGAAGGTGTACACAACCTATCCAACATAATC TACAAAAGAATCCGCATTCAAGGCTTTGTAGTGTCTGATTTCTACGACAAATACTCAAAGTTCTTGGAGTTTGTGCTTCC CCACATTAGAGAAGGGAAGATAACGTACGTGGAAGATGTAGCTGATGGGCTTGAGAAAGCTCCCGAAGCTCTTGTGGGAC TCTTCCATGGTAAGAATGTTGGGAAACAAGTTGTTGTTGTTGCTCGTGAGTGAAACTATGTTTGATACTTGTACTTTATC CAATTAGAGTTTGTGTGGGTGGTTTTTGTTTTATGGATAAAGATAATGTTTGTTATGGTTTCGAATCAAATA ......N.................................................i....................... ..............................N.....................N........................... .......................................................i........................ ................................................................................ .........................................N............N...........i............. ........................................................N....................... .................................................N...........................N.. ...............................N..................................i........i.... .....N...........N...................................N.......................... ..........................i..i.........N........................................ ......................N..N...................................................... .....N.......................i.............i......N................i.....i...... ....................N........................................................... ................................................................................ ...................................N........N................................... ......N........N.........................................N...................... ................................N............N........N................. Pos Score Pred ------------------------ 7 0.500 - 57 0.816 Yes 111 0.369 - 133 0.141 - 216 0.770 Yes 362 0.269 - 375 0.421 - 387 0.742 Yes 457 0.367 - 530 0.354 - 558 0.191 - 592 0.427 - 627 0.809 Yes 636 0.727 Yes 646 0.173 - 658 0.104 - 694 0.077 - 747 0.555 Yes 750 0.519 Yes 760 0.176 - 823 0.208 - 826 0.358 - 886 0.184 - 910 0.659 Yes 924 0.783 Yes 931 0.397 - 948 0.744 Yes 954 0.664 Yes 981 0.144 - 1156 0.195 - 1165 0.113 - 1207 0.099 - 1216 0.151 - 1258 0.065 - 1313 0.055 - 1326 0.053 - 1335 0.037 -

Explain the output -- Processing locus 585: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTCCGATCTCACCGGATCAGCCGTTGTACTCTTACCAATCCGTCGTCTAGCTTGAAATCTCCGAGCTCAAAGGTGTGT TACAGTCACTAGTCACTGTGATTTCTCACGCTTACATTACAAAATCGGAGCTTGTATTGTGTGATTAGATTTGGTTCATT AATGGTGGGTTTTGTGTTCAGAGGTGGATGGTCCATGGCTGAGCCAGAATTAGTAAACGGAGGAGTGAAAGAGAACAAAT TATGGAAAGGTGTATTCGCAGTTTCCGGCATTATGTCTACTCTTGTAATCTACGGTGTTCTTCAGGAGAAGATAATGAGA GTTCCTTATGGAGTGAACAAGGAGTTTTTTAAGCACTCTTTGTTTCTTGTTTTCTGCAATCGGCTTACTACATCAGCTGT CTCTGCTGGTGCTTTACTGGCAAGCAAGAAAGTTTTGGATCCTGTAGCTCCGGTTTATAAATATTGCCTTATATCAGTGA CTAACATCTTAACTACAACATGTCAATATGAGGCTCTCAAGTATGTGAGCTTCCCTGTCCAAACTCTTGCAAAGTGTGCC AAAATGATACCAGTAATGGTGTGGGGAACGCTCATTATGCAGAAGAAGTATAAGGGCTTTGACTATTTAGTGGCTTTTCT GGTGACCCTTGGCTGCTCTGTCTTTATTCTCTTTCCGGCAGGAGATGATGTTAGTCCATACAATAAGGGAAGGGAAAATA CTGTTTGGGGTGTTTCCCTTATGGCTGGTTATCTTGGGTTTGACGGCTTTACAAGTACATTCCAAGACAAGCTCTTTAAA GGATATAACATGGAGATACATAACCAAATATTCTACACAACACTTTGTTCTTGTGTTTTGAGTTTCACTGGACTTATATT GCAAGGGCATCTACTACCAGCTGTAGATTTTGTTTCTCTGCATAGGGATTGTTTACTCGACATCGCTCTGCTTTCCACAG TTGCAACAGCGAGCCAGTTCTTCATTTCTTACACAATTAGGACATTTGGTGCTCTAACATTTGCTGCAATCATGACGACC AGACAGCTTGCAAGCATCATGCTCTCATGCATTTGGTTTTCTCATCCTCTTAGCTGGGAGCAATGTATCGGATCGGTCAT CGTTTTCGGGTCTTTATATGCGAAAAACTTACTGAACAACAAGAAAAATTCACAAACGCAACCACCACCTCCAGAACTTC CGCAGTACGAAAAGGTTGAGAGCTCTTAAGAAGAAATATAACACCAGTTAAAGGTTTTTGCGCTTGATATAACCCTCAGA AGTTCAGAACAAAAGAAAACTAGCCGTTAGGAAACTGAAACTAGGATT ................................................................................ ................................................................................ .......N........................................................................ ................................................................................ ...................i.......i..............i..................................... ...N...........N....................i........................................... ............................................N..N................................ ....................N........................................................... .........N...................................................................... ................................................................................ .......................................................................N........ ..................N.......i...................................i................. .................N.............................................................. ................................................................................ ................................................ Pos Score Pred ------------------------ 162 0.342 - 188 0.185 - 195 0.471 - 242 0.365 - 273 0.353 - 315 0.328 - 328 0.307 - 500 0.803 Yes 508 0.504 Yes 523 0.557 Yes 564 0.308 - 576 0.467 - 597 0.521 Yes 685 0.204 - 688 0.239 - 741 0.461 - 810 0.375 - 1032 0.403 - 1059 0.453 - 1067 0.538 Yes 1103 0.752 Yes 1138 0.193 -

Explain the output -- Processing locus 586: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCAAAGAAATTAAAATACAATCTTAGTAGCATGATTCGCTATCACAAATTCTCAGGTTAACTAAAATTAAAGATCCTCC CCACATACAAAAAGATTGTAGTCTCCCTTATTTTGCTCATTACCCTTTCTTTTAAATAGCCTTTTAATATTCTTCATAGA ATCAATTTCAAATCTAAACTTAAACTCTGGCCTCTCTTATATGACAACTTTAATGAATAAGATCTTACTTCTTTTCTTCA ATAAATATTTCCTCTGTTCAATTTCTTGTTTTATTTTATTTTTGAAGAATATGGTTGAAAGAATGTCTAAAGCTTGTTGG TTAGTTGCTGCCATCATATTTACAGCGGCCACGGTGGTTTACGGACAACAAGCGCCGTGTTTCTTCGTTTTTGGAGACTC AATGTCCGATAATGGCAATAATAATAACTTGAAATCCGAGGCAAAAGTCAACTTTTCACCTTATGGTAATGACTTTCCTA AAGGCCCGACCGGCCGTTTTTCAAATGGCCGCACTATCCCTGATATTATCGGTGAATTATCCGGTTTTAAGGATTTTATA CCGCCATTCGCCGAAGCATCACCTGAACAAGCTCACACCGGGATGAACTACGCTTCCGGTGGAAGTGGACTTCGTGAAGA AACTAGCGAACACTTGGGAGATAGAATCAGTATAAGAAAGCAACTACAAAATCACAAGACGTCCATAACGAAAGCGAATG TACCAGCAGAAAGATTGCAACAATGTCTCTACATGATCAACATTGGAAGCAACGATTACATCAACAATTACTTCATGTCA AAGCCGTACAACACTAAACGACGCTACACTCCTAAACAATACGCATATTCTCTCATTATTATTTACCGTTCCCACTTAAA GAATTTGCACCGTCTAGGAGCGAGGAAGGTGGCAGTTTTCGGACTCAGCCAGATTGGGTGCACACCAAAGATAATGAAGT CACACAGTGACGGTAAAATCTGTTCCCGCGAAGTGAATGAAGCAGTGAAGATTTTTAATAAGAACCTTGACGACCTTGTC ATGGATTTCAATAAGAAGGTCAGAGGTGCAAAGTTTACCTACGTCGATCTTTTCTCCGGTGGAGATCCCCAAGCCTTCAT TTTCCTAGGATTTAAGGTTGGAGGTAAGAGTTGTTGCACAGTAAACCCGGGGGAAGAACTTTGTGTCCCAAACCAGCCGG TTTGTGCAAACAGGACTGAATATGTCTTTTGGGATGATCTTCACAGCACGGAAGCCACTAATATGGTAGTGGCGAAGGGC TCGTTCGACGGAATCATAAGTAAACCGTATAGTATCGCCCAGCTTGCGAAGGAATAGAGGCTAGCCCCAACATATAGACA TGAAAAATGATGACATTTTGAGTTTAGTAGATCTTTAACAGGGAAAATAAATTTGCATGTATATTCAATGAACCAA ...............................N................................................ ................................................................................ ........................................N...........N........................... ..................................................i...........i................. ................................................................................ ........................N....................................................... ..........................................i..................................... .............................................................................N.. ......................i.........N.........................................N..... ................................................................................ .........................................................................i...... ....................................N........................................... i............................................................................... ................................................................................ .....................N...........N............................i................. ...............................................................................N ......N..N..............................................N..........N........ Pos Score Pred ------------------------ 32 0.196 - 201 0.219 - 213 0.290 - 291 0.775 Yes 303 0.645 Yes 402 0.644 Yes 412 0.601 Yes 463 0.246 - 469 0.439 - 505 0.386 - 603 0.590 Yes 718 0.282 - 743 0.521 Yes 753 0.293 - 795 0.312 - 954 0.553 Yes 997 0.100 - 1041 0.526 Yes 1222 0.488 - 1234 0.157 - 1263 0.876 Yes 1360 0.114 - 1367 0.112 - 1370 0.081 - 1417 0.048 - 1428 0.039 -

Explain the output -- Processing locus 587: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGTATATATACTATATTACTTTTAGATAGTGAAAATTGGTTATTAGTTGTCGTTTAGAAAGAAGGAAATTTTAAGAAAA AATACTGATCGTAAACTATAACCAATGTATGTATTAGTATACTTTGATACTTCAAACACACGTGTGTGGTGCGGGGATGA GACAGAGAAAGAGGTTGGTCTTGTTCTTGTCTTTGACTCTAAACCAGTCTTTTTCGATACATTTTTCTTCACTCACAAGT CTATCATCATGTTTCTAACGAAGACATTTATTTTATTTATATTTTGTAACAAAAAAATGAAGACCCACCTCTTGCTTCTT CTTCACATCCCCATTTCATCTTCTCTCTGTCTCTCTCTATTAGAGACTCTCTCTACTCTACCCATCAATCTCAACAAACA CTACTTTCTATCTCTTTCTCTCTTTGTCATATCCATTACGCATATTCGTATCATTCCAAAGCAATCCCCACAAATCATAT CATCCTCTCCATCTTTCCTTGCTTCTTACAATCTCTCCAATTTCAAATCTGTATACTCTTCTTCAAAAAGGCTCCACCAG TCCAAAATGATGATGAGGAAACCGGACATTACTACGATCAGAGACAAAGGCAAGCCAAATCATGCATGTGGTGGTAATAA CAACAAACCGAAGCTAAGAAAAGGACTTTGGTCGCCTGATGAAGATGAGAAGCTGATAAGATACATGTTGACTAATGGAC AAGGATGTTGGAGTGACATCGCTAGAAATGCTGGTCTTTTACGTTGTGGTAAAAGTTGTCGCCTTCGCTGGATCAATTAC TTGAGGCCTGATCTTAAACGTGGATCCTTCTCTCCTCAGGAGGAGGATCTCATCTTCCATTTGCATTCCATTCTTGGTAA CAGGTGGTCTCAAATAGCTACTCGGCTTCCAGGTAGAACAGACAACGAGATCAAAAACTTTTGGAACTCGACATTGAAGA AGCGGCTTAAGAACAACAGCAACAACAATACTTCATCAGGATCATCACCTAACAATAGTAATAGTAATTCCTTGGACCCA AGAGATCAACATGTGGATATGGGAGGCAACTCAACTTCATTGATGGATGACTATCATCATGATGAAAACATGATGACAGT GGGGAACACCATGCGCATGGACTCTTCCTCCCCATTCAATGTTGGACCAATGGTTAATAGTGTGGGCTTAAACCAACTTT ATGATCCCTTGATGATATCAGTGCCGGATAACGGATATCACCAAATGGGAAACACAGTGAATGTGTTCAGCGTTAATGGT TTAGGAGATTATGGAAACACAATTCTTGATCCAATTAGCAAGAGAGTATCAGTAGAAGGTGATGATTGGTTCATTCCCCC CTCGGAGAATACCAACGTCATTGCTTGTAGTACAAGCAACAACCTAAACTTACAGGCCCTTGATCCTTGCTTCAATAGCA AAAATCTTTGTCATTCAGAAAGCTTCAAGGTAGGGAATGTGTTGGGGATAGAGAATGGTTCTTGGGAAATAGAAAACCCT AAAATCGGAGATTGGGATTTGGATGGTCTCATCGATAACAACTCTTCTTTTCCCTTCCTTGATTTCCAAGTCGATTGACA ATTTCTCTCTTTTCATTTCATTCTATCTTCTTTTACTCATTCCTTCTTATTCATTCTTATACACATATTTTTTAACATGA AGTGGATCGATCTTTTT ................................................................................ ........................i...N...............................................N... ................................................................................ ........N...............................................i....................... ................................................................................ ................................................................................ ................................................................................ ......i..i..i................................................N...N.............. ......................................N.....N...................i.........N..... ....N......................i.................................................... ................................................................................ ................................................................................ ................................................................................ ..........N.......i.......................i...N...........N..N.......i..N....... ..........i.....N.....................N..........i.............................. N..........N................................i...............N..............i.... ..........N..................................................N.................. ................................................................................ ....................................N.................N......................... ......................N......................................................... ............................................................................N... ................. Pos Score Pred ------------------------ 105 0.810 Yes 109 0.116 - 157 0.131 - 249 0.302 - 297 0.570 Yes 567 0.748 Yes 570 0.709 Yes 573 0.890 Yes 622 0.497 - 626 0.269 - 679 0.147 - 685 0.348 - 705 0.537 Yes 715 0.467 - 725 0.204 - 748 0.508 Yes 1051 0.163 - 1059 0.630 Yes 1083 0.605 Yes 1087 0.269 - 1099 0.117 - 1102 0.085 - 1110 0.524 Yes 1113 0.479 - 1131 0.536 Yes 1137 0.340 - 1159 0.258 - 1170 0.581 Yes 1201 0.211 - 1212 0.315 - 1245 0.815 Yes 1261 0.293 - 1276 0.508 Yes 1291 0.142 - 1342 0.230 - 1477 0.367 - 1495 0.270 - 1543 0.104 - 1677 0.068 -

Explain the output -- Processing locus 588: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCCATGAAATGAATGTGTTGAAAGGTGACCATTTTAAGACAAGAGGGACACATGAGCAAAACATACAATTTGACTTGTC AATTTGTTAAGGTTAATTTGACTAGTGTCCGTTTATATTTTTGGAAATCAAAGCGTGTGGAAGGAAAAGAGTCATCATAT TTTTCTAAAACAGCTCCAAGAAATCTGGAAATTCTCGATAAACAACTTACAACTACATTTTAGGGTCAATAAAAGTAAAA CAACAATATTTATTATAAAGAAGGCTGAGATGCAGACCCCATCGATGGCTGCCTCCACTACTTCCTACTACCCAATCCCC AAAAGCTTCCTTCTTTCTCCTCCTCGTCACAAACGAAACCCTAATCTCATCTCCTGCTCCACCAAACCCATTTGCTCTCC TCCTCCTCCTTCGTCTTCTTCTTCGTCTCCTCTGCAAACGACGACAACCCATCGAAGCCAAAAGCAGAATCTGCGTCTAC CCACGTTTGAAGATTCGTTTCTTCTTTACCAATTTAGCTCTCCCACCGAAGACCCAGGATTCTCTAACCGGATCCCGGAA CAATTCGACGGAGAGCCACGCGAATTGGTGTTGCCGAGAGTAGAAGACAACAACAAAGGGTTGGCAATTTCGTCGAACAT GTGGTGGGCAGATCTGAAAGCGGCTCTTGGGCAACGAATCAATATTGAGGGTATCGTTTCTTCGGTCTCCGTTGTTGTTA AAGATCGTCAATTTGTTCTTCCGCATGTTTCTGTGAAAGATTTAAGATACATTGATTGGGAAGTGCTGAAGAGAAAGGGC TTCAAAGGTGTAGTGTTTGATAAAGATAACACTCTTACGGCTCCTTATTCTCTAGCGATTTGGCCACCATTACGTCCTTC CATTGAGCGGTGTAAAGCCGTTTTTGGCCATGACATCGCCGTGTTTAGCAATTCCGCTGGGCTCACGGAATACGATCATG ATGATTCGAAAGCTAAAGCATTGGAGGCTGAAATAGGAATTAGAGTGTTGAGGCATAGAGTGAAGAAGCCTGCAGGAACT GCTGAAGAAGTTGAGAAACACTTTGGTTGCACATCTTCAGAGCTAATCATGGTGGGTGATCGACCTTTCACAGATATCGT TTACGGGAACAGAAACGGATTTCTAACCGTATTAACCGAGCCTTTGAGTCGAGCTGAGGAGCCTTTCATTGTTAGACAGG TGAGGAGATTAGAGTTGGCCTTGTTGAAACGTTGGTTGAGAAAAGGTTTGAAGCCTGTGGATCATAGTTTGGTATCAGAT ATAACACAGTTTGTTAAAGTTCCATCAGATTTGTAAGAAGGATTCTCATTTTGCTATTGCAACAACAACTTTGTTCCTTG TTTTTTTTTTGTAATTGAAATTTTGAGTTTGGGTTTTGGCCAAATGCATCATGAT ....N....i...N......................................N........................... ................................................................................ ................................................................................ .............................N..............i................................... ................................................................................ ................................................................................ ................................................................................ ..............................................................................i. ................................................................................ ........................N....................................................... ................................................................................ .............................N...............................................N.. N............................................................................... ................................................i............................... ................................................................................ ................................................................................ ................................................................................ ...........................................N......N.... Pos Score Pred ------------------------ 5 0.345 - 10 0.647 Yes 14 0.468 - 53 0.415 - 270 0.464 - 285 0.767 Yes 639 0.681 Yes 745 0.125 - 910 0.219 - 958 0.341 - 961 0.149 - 1089 0.564 Yes 1404 0.048 - 1411 0.043 -

Explain the output -- Processing locus 589: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCAGTTTCACGTTGACAGGAGAAGAGATTTTTAGTGGAGATGCATTAGCTGAGTGTAGAAAAGGGAGATTCAAGAAGCT GCTTTTGTTCTCTGGGAATGATTATTTGGGTTTGAGCTCACATCCTACAATATCAAACGCTGCTGCAAACGTATGTTTTC AAAACAATTTCATGTCAACGAACTTAAGTATAGATTTCTTCCACTGTCTTATGTTTTTTCTTCAAGGCAGGCAGTCAAAG AATATGGTATGGGACCTAAGGGTTCTGCTTTAATATGTGGCTATACCACTTATCATCGTTTGCTTGAGTCTAGTTTGGCG CAACTGAAGAAAAAAGAGGATTGTCTTGTTTGTCCTACTGGGTTTGCTGCCAATATGGCTGCAATGGTTGCAATTGGAAG TGTTGCTTCTCTTTTGGCCGCTAGCGGGAAACCTCTGAAGAATGAAAAAGTTGCCATCTTTTCTGATGCGCTGAATCATG CATCAATTATTGATGGTGTCCGTCTTGCTGAACGACAAGGAAATGTTGAAGTTTTTGTTTATCGACACTGTGACATGTAT CACCTTAATTCGTTATTATCAAATTGCAAAATGAAGAGGAAGGTCGTGGTGACTGATAGCTTATTTAGTATGGACGGTGA CTTTGCACCAATGGAAGAGCTCTCTCAGCTTCGGAAGAAGTATGGCTTCCTTCTAGTTATTGATGATGCTCATGGAACAT TTGTCTGTGGAGAAAACGGTGGTGGCGTGGCTGAGGAATTTAACTGTGAAGCTGATGTAGATTTATGTGTGGGCACTTTG AGTAAGGCAGCAGGGTGTCATGGCGGTTTCATAGCTTGCAGCAAAAAATGGAAGCAACTGATACAGTCGAGAGGTCGTTC ATTCATATTTTCAACAGCAATCCCTGTCCCAATGGCTGCAGCTGCTTATGCAGCAGTTGTAGTGGCGAGGAAGGAGATAT GGAGAAGAAAGGCAATATGGGAGAGGGTAAAAGAGTTCAAGGAATTATCTGGAGTTGACATCTCAAGCCCCATTATCTCA CTTGTTGTAGGGAATCAAGAGAAAGCCCTCAAAGCGAGCCGGTATCTATTAAAATCAGGCTTCCATGTAATGGCAATACG ACCGCCCACAGTGCCACCCAATTCTTGCAGGCTAAGGGTGACACTGAGTGCAGCACATACCACAGAAGATGTGAAGAAAC TCATCACTGCGCTTTCTTCTTGTTTGGACTTTGACAACACAGCCACTCACATTCCTTCCTTTCTATTTCCCAAATTATAA AACTTCTTTCTGTTGTCGCAATTATTCCAAAGCTCTAATTCTAAAATTGTTTCTGATATTGTCAGTGAAAGAATCACTTG AAGTACAGAGTTCATTCAT ........................................N....................................... .................N......................................................N....... ...........i......................................N............................. ...i....i.........................N............................................. ......................................................i........i................ .........................................N.......................N...........N.. ............N.............................N...............................i..... ..............................i......................................i.......... ..........i..............................N....................N..N.....N........ ......................................................N.........N............... ...................i...........................i................................ ...............................i...............N..............................i. ................i............................................................... ................................................................N....i.......... ....................................................................N........... ................................................................................ ................................................................................ ................... Pos Score Pred ------------------------ 41 0.276 - 98 0.198 - 153 0.407 - 172 0.653 Yes 211 0.261 - 244 0.585 Yes 249 0.671 Yes 275 0.194 - 375 0.789 Yes 384 0.835 Yes 442 0.102 - 466 0.332 - 478 0.134 - 493 0.184 - 523 0.324 - 555 0.509 Yes 591 0.759 Yes 630 0.526 Yes 651 0.811 Yes 682 0.358 - 703 0.271 - 706 0.199 - 712 0.312 - 775 0.178 - 785 0.413 - 820 0.595 Yes 848 0.789 Yes 912 0.516 Yes 928 0.184 - 959 0.582 Yes 977 0.627 Yes 1105 0.284 - 1110 0.797 Yes 1189 0.196 -

Explain the output -- Processing locus 590: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATATCCGACCTGTTTATTAAAATGAAGGCCCATAGCCCAAAGTACGGTCCATAATTCGGCCCAAACTCGATTTTAAAA TTGGTGTATGTAAGTTTCTAGACCTGTCTTCTTCAATCAAAAGTCAAAACCCTAATTTGGAATAGCTTTCTGAAGATGAT GCCGGAGAAGACGATTATTTCGTCGGAGAGTTCAAATTCGTCTCCGTTAAACCCTTCTTCTACGCGGATTATCTGCCATG TATGTAATAAGCAGTTCTCGCAGTACACTTGTCCTCGCTGCAACTTCCGGTACTGTTCCCTTCCTTGTTACAAATCTCAC AGTGTCCAATGTACTGAATCATTCATGAGAGATAATGTAAACGATGAATTGAAGCAAGTTCGATCTGATGATCAAACGAA AAGGAAGATGCTCGAAATTTTGAAAAGGTTTCACGAAGAAGAAGAGGAAGATGATGGGGGAATTGATAGCATCACGGATG ATGAAGGTTTGAGCTTACCGGAGGAGATTATTCAGAAGATAATGAACGGAGATGAAGTGAGTCTTGATGATTTGAGCTTA GAAGAGAGAAAAGGATTCCAAAGAGCCTTAGCTTCTGGAGAATTAAGCAAAATGATCCAACCATGGGATCCTTGGTGGTT ACTTGCTTCAGCTCGAACGATCAATCTTGGACTCGGAGGTACACAACTTGTTCAATGCGTTGAAGACGAAGAAGAGACCA CAGTTGTGAGTGAAATCCCACGAGGGCCTGATACACCTTTGATCTCACTAAGCAAGCTTACCTCCACGAATCCATCTCCT CTCTTACCAATCCATTTGATTGACATTGCATATAGTTATTGCTTTACGCTCCGTATCTATAATGGAGAGTGGCAATCTGA TTCGCTTGGTGCAGCAACAATGGTGTTGACTGTTTCATCAGTTTTGGGTCACAATGGACAACCAGAGACCATAAAGGAAG TGTTGTCCTTTTGCTTGGAACAGACATGCTCTTCAGCTTATAAAAACCTTGGTGGTGGTCTGAAGTTCGGATTGAATCTG GTGGATGATGTGATTTGTTTTCTTTCTCTTGGCTCTGGAGCTATGGTATGTCTGCTCGGTGATCTCCAAAGGTTGATTCT TGGTGCAATAAAGGAAGTAAAATCATCATCAGGGCGTGATCTGAAGAAGAAACTAAAGCTTGCAGAGAGGAAAGTGTTCT ACATGATGTGTTGGGTCAATGAGCAGAATTCTGAAGTATGGGAAGCTCTGGAACCATCTGTAAGGGCAGAGAAGAACTCG GTGGTTGAGCTCAACGATTACAGAGGGTTTCCAGAAATGAAGAAGAAAGATCAATTTCGAAAAGGTGGTGGCGTGGTGAT TGAGGAGATAGAATGAGTTCTGATGTCGTAGTTACATAGCTTGATGATGTAAGGTATGTTAACAAATGAATATCTTGTGG TGTTCCTAATTAAAAAATGTAAGGTGTGTTAACTA .......................N........................................................ .......N...................................................................N..N. .............................................................................N.. ........N...............i.........N........N.......................N............ .......i..........................................N..N.......................N.. N........................................N.........N..............N............. ...................................................i..........N................. ......................................................N......................... ................................................................................ .............................................................i.................. ...................i.................................N.......................... .........................N...................................................... ....N..N..................................N....i................................ ................................................................................ ..N..N............N..................i.......................................... ....................................N........................................... ............N.........N....................N..N........N.........N.............. ................N.................. Pos Score Pred ------------------------ 24 0.429 - 88 0.145 - 156 0.378 - 159 0.411 - 238 0.435 - 242 0.167 - 329 0.289 - 345 0.655 Yes 355 0.474 - 364 0.417 - 388 0.111 - 408 0.641 Yes 451 0.380 - 454 0.262 - 478 0.128 - 481 0.190 - 522 0.434 - 532 0.181 - 547 0.161 - 612 0.594 Yes 623 0.404 - 695 0.264 - 862 0.539 Yes 900 0.672 Yes 934 0.239 - 986 0.110 - 1045 0.254 - 1048 0.279 - 1083 0.402 - 1088 0.659 Yes 1203 0.443 - 1206 0.394 - 1219 0.151 - 1238 0.733 Yes 1317 0.292 - 1373 0.375 - 1383 0.168 - 1404 0.034 - 1407 0.083 - 1416 0.049 - 1426 0.079 - 1457 0.085 -

Explain the output -- Processing locus 591: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTGTCTCTGCTGAATCATATTCTGGAACTCAAGTATAGATTTTTTTTTTGCTTCATAGTCGTTTATGTATGTTCACTA ATACTAGCAATGCTTTGGTGATTGGCTATTTTGCTTCATAGTAGTTTATTTATGAAATGGTCTTTTTTGACACTGTTTAA TGATTGGCTATGTGCAGGAGGATTAAAAGATGGACCAGTCTTTTATTTTGATATTCCGTTAGCTTGTTCAGTTATTGTAG AAGCTGTACCGTCAAGACTTTTAATATCTCAAATGGATGATGGTGGGCATCGTGAGAATGGTCGGCATAAAGCAGCAGTT CAGGGCCAGTGGTTGATGCAGCATCAGCCGTCGATGAAACAAGTGATGTCGATAATAGCAGAGCGCGATGCAGCGATTCA GGAGAGAAATCTGGCGATATCAGAGAAAAAAGCAGCAGTAGCTGAAAGGGACATGGCGTTTCTTCAGAGGGACACCGCCA TTGCTGAGCGCAACAATGCGATAATGGAGAGAGACAGTGCTCTTACAGCACTCCAATACCGTGAGAACTCTATGGTTACT GCTCCTGCTGCCAATATGTCAGCATGTCCACCAGGATGTCAAATATCACGTGGTGTAAAACACTTGCACCATCCGCACAT GCATCATCATCATCAACAACATCACATACCTCAGTTGACTGAAAATGCATATGAAACCAGAGAGATGGAGCCAAATGATG GTCTCCCAACATCACCACCTGCCGGATCCACCTTGGAATCTGCCAAACCAAAACGTGGTAAAAGAGTGAACCCAAAGGCG ACAACACAAACTGCTGCTAATAAGAGAGGGCCAAAAAATCAGAGGAAGGTTAAGAAAGAGAGCGAGGACGACTTAAACAA GATAATGTTTGTAAAGACGACCCATGACTACACAGACGAAGATTCAAGTAAGCATATCTTAATAGGATCAAAATCTGACT GGAAAAGTCAAGAAATGGTGGGGCTGAACCAAGTGGTTTACGACGAGACAACGATGCCACCACCTGTATGTTCATGTACA GGAGTTCTCAGACAGTGCTACAAATGGGGAAATGGAGGTTGGCAATCATCGTGTTGCACAACTACACTATCAATGTATCC TTTACCTGCACTGCCCAACAAAAGGCATGCTCGAGTTGGTGGACGGAAAATGAGCGGAAGCGCGTTCAACAAGCTTCTAA GCCGGCTTGCTGCAGAAGGGCATCATGATCTTTCAAACCCGGTCGATCTGAAGGACCATTGGGCAAAGCACGGTACAAAC CGCTACATTACGATTAAATGATATGTGCATTTAGGTGTGAGTTGTTCTTACGACATTATCTGATTTTAAATCATGTGTAC TAAATTCAAATAAACTCGAGAGGAAAAGAATTGTATGTGG ..................................................................N...N......... .........N.........................................N....N......................N .........N...................N.................................................. ................................i...N..i.................N...................... ...............i.................N...........i.....................N............ ....................................................i........................... ...............N.......i...............................................N........ ...............N.......i...........N..........................................i. ............................................i.....N.............i.........N..N.. ................................................................................ ................................................................................ ....i..................i........................................................ ..............i......................................N.............N.....N...... .......................i.......i........................................i....... ..........................N......................i.............................. ........................N....................................................... .................N....N.................................................N....... ..................................N..... Pos Score Pred ------------------------ 67 0.258 - 71 0.456 - 90 0.316 - 132 0.127 - 137 0.211 - 160 0.268 - 170 0.321 - 190 0.471 - 273 0.695 Yes 277 0.228 - 280 0.693 Yes 298 0.420 - 336 0.606 Yes 354 0.354 - 366 0.641 Yes 388 0.180 - 453 0.717 Yes 496 0.201 - 504 0.805 Yes 552 0.318 - 576 0.380 - 584 0.574 Yes 596 0.483 - 639 0.569 Yes 685 0.834 Yes 691 0.354 - 705 0.821 Yes 715 0.427 - 718 0.481 - 885 0.546 Yes 904 0.541 Yes 975 0.802 Yes 1014 0.213 - 1028 0.275 - 1034 0.168 - 1064 0.558 Yes 1072 0.788 Yes 1113 0.683 Yes 1147 0.484 - 1170 0.684 Yes 1225 0.170 - 1298 0.285 - 1303 0.446 - 1353 0.063 - 1395 0.055 -

Explain the output -- Processing locus 592: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTTTCAAGCTCTTCTTCTACGATTCCCCGAAGCTTCTGGTAAAAATCTAAACGAAATTCCCAATCTTGTCCTTAGCAAA GTTTCGGTTTTTACCTCTTGACAATTTTCAATGACATTTAATGCGTACTCAGGATTGAGATCTCTCTCTCAAGCAATGGC AGCTACAAGTGAGAAACAGAACACCACAAAGCCTCCTCCTTCTCCTTCTCCTCTCCGCAATTCCAAGTTTTGTCAGCCCA ATATGAGGATCTTGATCTCTGGAGGAGCTGGCTTCATTGGTTCTCACTTGGTTGATAAGCTTATGGAAAATGAGAAGAAT GAGGTGGTTGTTGCTGATAACTATTTCACTGGCTCAAAAGAAAACCTCAAGAAGTGGATCGGTCACCCCAGGTTTGAACT TATTCGTCACGATGTTACCGAGCCTTTGTTGATCGAGGTTGATCGGATTTACCATCTTGCTTGTCCTGCCTCTCCTATCT TCTACAAATACAACCCTGTTAAGACAATCAAGACCAATGTGATTGGTACACTCAACATGCTCGGTCTTGCCAAGCGTGTT GGAGCAAGAATTTTACTAACCTCAACCTCTGAAGTGTATGGAGATCCTCTCATCCACCCTCAACCAGAGAGCTACTGGGG AAATGTCAACCCTATTGGGGTTCGGAGTTGCTATGACGAAGGCAAGCGGGTAGCCGAAACCTTGATGTTTGACTACCACA GACAACATGGCATTGAAATCCGCATTGCTAGAATCTTCAACACATATGGTCCTCGAATGAACATCGATGATGGGCGTGTT GTGAGCAACTTCATTGCTCAAGCACTCCGGGGTGAGGCATTGACAGTTCAGAAACCGGGGACACAGACCCGCAGTTTCTG TTATGTCTCCGACATGGTGGATGGACTTATCCGTCTTATGGAAGGCAATGATACTGGCCCTATCAACATCGGTAACCCAG GTGAGTTCACAATGGTGGAACTGGCTGAGACGGTTAAGGAGCTTATTAACCCAAGCATAGAGATAAAGATGGTGGAGAAC ACACCAGATGATCCAAGACAGAGGAAACCAGACATTAGTAAAGCCAAAGAAGTGTTGGGTTGGGAGCCAAAGGTGAAGCT CAGAGAAGGACTTCCTCTCATGGAAGAAGATTTCCGACTAAGGCTTAACGTCCCAAGAAACTAAAACCACAAGAAAACAC AGACGGTTTTGAGTTCACGAGAGTTGTCAGCTGTTGAGTTATATGATGATGCTATTATACTACAAAGTGGTGGGTTTTTG TTT ................................................................................ ..............................N.........N..................................i.... ................................................................................ ..i...........................................................i......N........N. ................................................................................ ...........N.................................................................... ....................................i...................i....................... .....................................N.......................................... ..i.............................N...............................i............... ......N......................................N..........i.........i..i.......... ................................................................................ ..N..........i......N................i.........N................................ ...........i........................................................i........... .......N........................................................................ ...................N............................................................ ..........................................N..N..N............................... ... Pos Score Pred ------------------------ 111 0.368 - 121 0.389 - 156 0.785 Yes 243 0.719 Yes 303 0.640 Yes 310 0.241 - 319 0.349 - 412 0.233 - 517 0.507 Yes 537 0.672 Yes 598 0.422 - 643 0.727 Yes 673 0.453 - 705 0.530 Yes 727 0.368 - 766 0.285 - 777 0.567 Yes 787 0.629 Yes 790 0.562 Yes 883 0.495 - 894 0.601 Yes 901 0.262 - 918 0.731 Yes 928 0.248 - 972 0.859 Yes 1029 0.615 Yes 1048 0.367 - 1140 0.362 - 1243 0.035 - 1246 0.048 - 1249 0.050 -

Explain the output -- Processing locus 593: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAATCTACATTAGATATTGGAAATCGAATCGAATCGTAGGAGCGTAGATCTTCACGCCTCGCGATTAACAACGACGCGT CGCGTTTGCTTGTTCCCGTAGGCACCAAGGCATCTATATAAACATATTGAGTTCACAATGTTTCACCCTCAATTCGACAA TACTTCTTCTTCGTGTGTTCTCTCTCTCGGAAAAAACCCTAGTTTCTTGCCGAATCAAGGCAAAGTCACGATGAAGCAAG CTTCTTCTTCTTCTTCGTCACGTAATTCAACAAGTTTAACAAATAGACTTAAAACCATCTTCAAGAAAGCTGAAGAGCTT TCGATTCTCTGTGCTATTGAGGTTTGCGTCATCTATTACGGACCAGACGGTGAACTGAGAACATGGCCTAAGGAGAGAGA GACAGTGAAAGACATGGCTTTGAGGTATAAAGAGGCCAGAAAACGCAAGAAGAGCCGCAATCTTCATGAGTTCCTCGAGA AGGAGAAGGACAAGGACAAGGGGAAGACGAATTTGAAGAAGAATTGGTATCCAAACTTTGATCATTACTCTCCTCAACAA CTCTCTCAATTGATTCAGTCCTTAGAACGGACTCTTTCTACTCTGCAAGAAAGGCTTCGTATTGTTGAGGCGCAGAAACT ACAGAACACAAACTTGGTGCATCAGAGTTTAACACCATCGTATCTGAACCAGACCCAACACTTGAATCCTAGCAAGTTCT CGCTGTTTATGTACAACCATGGAGATGCTACTCTCTCACAACTCCCACTGTCTGCTCCACACTCCAATCAACTCATCAAT TACCAGAATCACTTGATGCAGCATGGTTTTGGTCAGAACATGTGTTCGGACAACATCACCAACAACAACTTTGAACATCC TGGCGTGTCAAACACACAAGACTACTCACCGTTACTTTCGGTACAAGCATCTGCAGTGAATAACTACGGGTTGAATAATC ACTTGATGCAGCAACAGGATCAGCTTCATGGTTTTGATCAGAACATGTGTATGGTGAGTGAAATCATCAACAACAACAAC GGTCTACAACATCCTAATCTCTCAAACACGGTTCCACATGAATTCTCTTCTGATTTCAACCAGAACCCTTATGGTAATGC GGTCGGTAACATTAGCTTCTCTCAAGACATGTTTTCAAGCTATGATGCGAGCAGTCTGCTACAGACATCTTCTCTGCCAC CTCTCCACAACATTCCTAGCAGTTATTGTTTTCCTGGCAACTCAAGACTTCTCTGATAACAAAATCTGTCCAAAAGAATT TGTTTTTTTGAAGCTTCATTAAGAGTCAGTCAATAGTTAAGTAGCAAGCGGATCGGAGAGAGATTGAAGCTGTTT ................................................................................ .........................................................N...................... ......................................................................i......... ................................................................................ ..............................................................i................. .............i...................................................N.............. ................................................................................ ................................................................................ ................................................................................ ........N.........i.....N....................................................... ...............N......i................N........................................ ................................................................................ .....i.....................N................i.....i............................. .....................................N................................N.....N... ............................i............N..N................................... ................................................................................ ........................................................................... Pos Score Pred ------------------------ 138 0.468 - 231 0.536 Yes 383 0.540 Yes 414 0.781 Yes 466 0.207 - 729 0.367 - 739 0.751 Yes 745 0.299 - 816 0.391 - 823 0.642 Yes 840 0.447 - 966 0.536 Yes 988 0.148 - 1005 0.605 Yes 1011 0.543 Yes 1078 0.274 - 1111 0.133 - 1117 0.180 - 1149 0.524 Yes 1162 0.328 - 1165 0.471 -

Explain the output -- Processing locus 594: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAAAGGACGTTAGCACGAAACTCGTAAATAACGCATGTTTATTTTCCAAATAAATTTATAATAAAACTATTATACATCA AAACCCATTAATTCATCATAGTGGCAACCAAGTGTATATAGCAATATAAATCAATTTTTTAAAAACAACTTCTGTAAACA AAAGGAAAGTGAAGGCACTATGTGTCCCCACACGGTTCCATTGAGACAAAGGCATATGTATGTGTGTGAGTATAAATATA AGTTGGTTCTTGAACATCATTGCATGTTGATTAATCACACAGCACAAATAAGAGTTAGAAAAATGGGAGTGTACTTAGGT AAGTATTGTTATATAATGATAATAATGTTAGTGTTGGTGTTGGGAAAAGAAGTGAGAAGCCAGTTGTTAAAAAATGGTTA TTATTCAACTTCATGTCCAAAAGCTGAGTCGATAGTTAGATCCACTGTTGAATCTCACTTTGATTCTGATCCCACAATTT CCCCTGGCTTGCTTAGGCTTCATTTCCATGACTGTTTTGTTCAGGGTTGTGATGGTTCGGTTTTAATCAAAGGGAAATCT GCGGAGCAAGCTGCTCTACCAAATCTTGGTCTAAGAGGTTTAGAAGTGATCGATGATGCTAAAGCCCGGCTTGAGGCGGT GTGTCCAGGAGTTGTGTCATGTGCAGATATACTTGCACTTGCTGCTCGTGACTCTGTTGACTTGAGTGATGGGCCAAGCT GGCGAGTACCAACGGGTCGTAAAGACGGGAGAATCTCGTTGGCAACTGAAGCATCGAATCTACCTTCTCCACTTGACTCT GTTGCTGTTCAAAAGCAAAAGTTTCAAGATAAAGGATTGGATACTCATGATCTAGTTACTCTACTAGGTGCACATACAAT TGGACAAACCGACTGTCTCTTCTTCCGTTACCGTTTATACAACTTCACGGTGACCGGAAACTCCGATCCAACCATAAGCC CATCGTTTCTTACACAGCTCAAGACTCTTTGTCCTCCCAATGGAGACGGTTCGAAACGTGTTGCTCTTGACATTGGTAGC CCCTCAAAATTCGATGAAAGTTTCTTCAAGAACTTACGCGACGGAAACGCTATTCTAGAGTCGGACCAACGGCTTTGGTC GGACGCCGAGACAAATGCCGTGGTGAAGAAGTATGCGAGTCGTCTTAGAGGTTTGTTAGGGTTTAGGTTTGATTATGAGT TTGGTAAAGCTATGATTAAGATGAGTTCCATTGATGTAAAGACTGACGTTGATGGCGAGGTTAGGAAAGTTTGTTCCAAG GTGAACTAGTTTGAATAAAGACATATAGTATATGTATTCGGTTTCTCTATGTTTCAGATTTCTAATCACTGAATGCATGT TACGTAATTGTCGTCTTTCAATATTTAT ...................................N............................................ ................................................................................ ...................i...................................N...N.................... .......................N......................................N................. ...............N........N................................................N...... ............N................................................................... ...........................N.......................N............................ ....................................................N..N........................ ..................N.................................................N........... ................................................................................ ..............................................N................................. ................................................................................ .......................................i........................................ .............N.................................................................. ..............N.................N.........................................N..... ...........N........i............N.................N............................ ...............................N................N.......................N...N... ............................ Pos Score Pred ------------------------ 36 0.180 - 180 0.582 Yes 216 0.403 - 220 0.340 - 264 0.116 - 303 0.415 - 336 0.207 - 345 0.449 - 394 0.489 - 413 0.192 - 508 0.231 - 532 0.363 - 613 0.277 - 616 0.151 - 659 0.473 - 709 0.312 - 847 0.293 - 1000 0.574 Yes 1054 0.380 - 1135 0.311 - 1153 0.084 - 1195 0.182 - 1212 0.346 - 1221 0.504 Yes 1234 0.143 - 1252 0.263 - 1312 0.053 - 1329 0.038 - 1353 0.059 - 1357 0.050 -

Explain the output -- Processing locus 595: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGCGATTTCTTTATGCTAAATTCGTATAATGTTTCAGATCTTGCTATGGGACTTGCTCTGTTTTCCTCTGATGGCAAA CTAATTTGGAAGGGGAGGTGAACTAACTTGGTAAGGGATTTGTTTGATGGGCAATTCTTTACAAGCCAAACCACTTCTAA TACATTTTGTCTTTCAACGATTTGTTTCAGCACACAAACTGGCAAAAGACGCCCCCAGGAAGAAGCCACAATGAAGCACA GCGGTGGGAACCAAGCCTGCTATGTTCTTGGTCAAAAGACCCCAAGCATCCGAGATCTCTACTCGTTGGGACACAAACTA GGTCAAGGACAGTTTGGGACGACTTACATGTGCAAAGAGATCTCAACAGGGCGTGAATACGCATGCAAGTCTATAACAAA ACGAAAGTTGATATCAAAGGAAGATGTGGAAGACGTACGCAGGGAAATTCAAATCATGCACCATCTGGCTGGCTACAAGA ACATTGTCACCATTAAAGGTGCTTACGAGGATCCATTGTATGTTCACATCGTGATGGAGCTTTGCTCAGGTGGGGAATTG TTTGACAGAATTATCCAGAGAGGACATTACAGCGAGAGAAAAGCAGCTGAGCTGATAAAGATCATCGTTGGTGTTGTTGA GGCTTGCCATTCGCTTGGTGTCATGCATAGAGATCTAAAGCCCGAAAATTTCTTGTTGGTTAACAAGGACGACGACTTCT CTCTCAAAGCCATTGATTTTGGGCTTTCCGTGTTCTTCAAACCAGGTCAAATATTCGAGGATGTGGTTGGAAGTCCGTAT TATGTTGCTCCTGAGGTTCTGCTCAAGCACTACGGACCCGAGGCTGATGTGTGGACCGCAGGGGTCATACTGTACATATT GGTGAGCGGAGTCCCTCCGTTCTGGGCAGAAACACAGCAAGGGATATTTGATGCAGTGCTGAAGGGACATATTGACTTTG ACTCCGACCCTTGGCCCCTAATATCCGACTCTGCAAAAAACTTGATCCGTGGCATGCTATGTTCCCGGCCCTCTGAGCGA CTGACAGCTCATCAAGTATTGCGCCATCCTTGGATCTGTGAAAATGGAGTTGCGCCAGACAGAGCACTAGATCCTGCTGT GCTTTCTCGTCTTAAACAGTTCTCTGCGATGAATAAACTAAAGCAGATGGCTTTACGGGTAATAGCTGAGAGTCTCTCGG AGGAAGAAATTGCTGGACTGAAGGAAATGTTCAAAGCAATGGACACGGATAACAGTGGAGCGATTACGTTTGACGAGCTG AAAGCCGGGTTGCGAAGATATGGTTCAACCCTCAAGGATACTGAGATTCGTGACCTTATGGAGGCTGCAGATATTGACAA GAGTGGGACCATAGATTATGGTGAATTCATTGCTGCAACAATCCATCTGAACAAATTAGAGCGTGAAGAACATCTTCTGT CAGCCTTCAGGTACTTTGACAAAGATGGGAGCGGTTACATCACTATCGATGAGCTACAGCACGCATGTGCTGAACAGGGA ATGAGTGATGTCTTTCTAGAAGATGTGATCAAGGAAGTCGACCAAGACAATGATGGAAGGATTGACTACGGGGAGTTTGT AGCTATGATGCAGAAAGGCATTGTTGGAAGAACAATGAGGAAAAGCATAAACATGAGCATCAGGAACAATGCCGTCTCGC AGTGAGAAGCAAGCAATCTCACGATTATGTACAGGTTAAGCCTTTCTCACTTTTTCAAGGTAAATTCCATCAAATTCTTT CCCAAACTGACAAGTCTATACATC ..............i...............N................i........................N....... ..............................................i................................. ......................................................................i......... .....................N.......................................................... ...........................N..................................N................. .......................N...............................i........................ .......................................N.............i.......................... ................................................................................ ......................N......................................................... ............................................................i................... ..................................................N............................. .....................................................N....N..................... ...........................................i.................................... ............................i.................i................................. ..........................i...........i......................................... ...................N.....................................N...................... .................N.............................................................. ........................N.......................N...............N............... i......N..............N..........................N..N........................... ....N..N..........................N.................N...............N........... ..........................N..................................................... ........................ Pos Score Pred ------------------------ 15 0.501 Yes 31 0.246 - 48 0.592 Yes 73 0.117 - 127 0.545 Yes 231 0.542 Yes 262 0.385 - 348 0.276 - 383 0.239 - 424 0.188 - 456 0.653 Yes 520 0.231 - 534 0.548 Yes 663 0.489 - 781 0.542 Yes 802 0.437 - 847 0.193 - 931 0.465 - 1014 0.492 - 1019 0.291 - 1084 0.558 Yes 1149 0.515 Yes 1167 0.655 Yes 1227 0.626 Yes 1239 0.660 Yes 1300 0.386 - 1338 0.426 - 1378 0.469 - 1465 0.398 - 1489 0.289 - 1505 0.199 - 1521 0.677 Yes 1528 0.220 - 1543 0.188 - 1570 0.311 - 1573 0.220 - 1605 0.464 - 1608 0.482 - 1635 0.167 - 1653 0.352 - 1669 0.430 - 1707 0.055 -

Explain the output -- Processing locus 596: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGCTATTGTAAGAGTTATTAAAAGTTACTTTACACCACAAAGTGAAATGTTAAACCATTTATTGGGTTTTTTGGATAGG TCAAAACGCAGCGTGAGGTGAGGCCCATCAATGACCAAAACGCTGTCGTCGTATCTGCAGCGAGGAGAGAATATTCGGAA TCGAAAGGCTTCATCTTCTTCTTCTTCACCTATCTCTTTACTACTAGCTCAGGGTTTTTACTTTTCTAGGGTTTATCTAT CTCATTCCGCAATCATGGAGTCTCTGGGGAAGCTACAGCTACATCACCAGCCATTTCACCTTTCCTTCACTCACACCTCT TCTTCAACATTCCCCAAAAACCTCTTCAAATCCTCAATTCAACCAATTTCATCTCTCAAATCCGCTTCAATTAAAGCTTC GTCCTCCAAATTTCAAAACTCTATCACCCCTCTCCCAAAGTCCACACCTTTCCGTCTCTTCAAATCCACATGCATCACTC TAACAACCGCCGCGGCTCTGCTTTTGGCGAATCTCCATCTCAAATCGCCGGCGATTGCAGCTCCCATCGCGCCTCCGCCG TCAGTTGAAAGCAAAGAAAACGTTACCTTGGAAGAAGAAGAGAGAGCGCTTGATGAGCATTTGATTACTCACCCTTCTGA TGTCGACGCTCTTCGTTCATTGATGGAGGTAAAGATCAAATCTCGAAAGCTTACAGAAGCAGTAGAAGTGATCGATCGTT TGATAAAATTGGAACCTGAAGAAAAAGAATGGCCAGTTTTGAAAGCTAACATCTTTACCTACAGTGGAGATCTAGACTTG GCTAAAACTGGATTTGAAGAGATTCTGGCTAAAGATCCGCTTCGTGTTGAAGCTTATCACGGCTTGCTTATGGCTTATTC AGATGCAGGTCTTGATTTGAAAGAGGTGGAGAGTAGAATTGAGGAAGCAATGTTGAAGTGCAAAAAGGAGAATAATCAAA ACGATTTTCGGGATTTTAAGCTACTCGTGGCGCAGATTCGGGTCATTGAAGGAAAACATAGTGAAGCATTGAAGCTTTAT CAAGAGCTTGTAAAGGAGGAACCAAGAGATTTTAGGCCGTATCTTTGTCAGGGAATTATATACACATTGCTGAAGAAGAA AGACAAGGCAGAAGAACAGTTTGATAACTTTCGGAAACTTGTTCCAAAGAATCATCCTTATAGAGAGTATTTTATGGATA ATATGATAGCAACAAAGCTTTTTTCCGAGAAGGCGCAGCGTGAGATGGCTGAGGAGATGGCTGGATCAACTAGTTGATGA GAATATGTGTAATGAGGTGAAAAATCATGCTTAGAAGACTTGGAAAATGTGGTTTTGAGGATTTTCTGTTGCAGGTTTTT CTTGATCGTCTCAAGA ...............................................N................................ ..............................N................................................. ................................................................................ ..............N................................................................. ................................................................................ .....................................................................i.......... ................................................................................ ....................................................N..........................N ......................N......................................................... ............................i................................................... .....................................................................i.......... ..N..............................................N.............................. ................................................................................ ................................................................................ .........................................................................N...... ..i.........................................N...........N...................N... ....N......N..............N...................N................................. ................ Pos Score Pred ------------------------ 48 0.276 - 111 0.204 - 255 0.406 - 470 0.526 Yes 613 0.319 - 640 0.210 - 663 0.426 - 749 0.707 Yes 870 0.768 Yes 883 0.147 - 930 0.478 - 1194 0.382 - 1203 0.708 Yes 1245 0.424 - 1257 0.355 - 1277 0.118 - 1285 0.115 - 1292 0.111 - 1307 0.074 - 1327 0.080 -

Explain the output -- Processing locus 597: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAGGTGAAACATAACAAAACAGATGATATTGATAGATCATTTGATTTCATTCATTGACTGACTTGCACAGTTGCACCCC AGGCTTGGCTCTATAAATTAGGCGGTAAATGGAATGATTAAAACATATCATTTTTCACAGAATCATAGAAAAATCAAGAA AATGTCTGAATCAAGGAGCTTAGCCTCACCACCAATGTTGATGATTCTTCTCAGCCTTGTAATAGCTTCCTTCTTCAACC ACACAGCTGGACAAATCGGAGTATGCTACGGGATGCTAGGCGATACCTTGCCAAGTCCATCGGACGTTGTGGCTCTTTAC AAACAACAAAACATCCAGCGAATGCGGCTCTACGGCCCTGACCCAGGCGCTCTTGCCGCTCTCCGTGGCTCTGACATCGA GCTCATCCTCGACGTTCCCAGTTCAGATCTTGAACGTCTCGCCTCCAGTCAAACGGAGGCCGACAAGTGGGTTCAAGAAA ACGTTCAGAGCTACAGAGATGGTGTCAGATTCCGGTACATCAACGTTGGAAATGAGGTGAAACCCTCAGTTGGGGGGTTT CTCTTACAAGCAATGCAGAACATCGAGAACGCGGTTTCTGGAGCAGGGCTTGAAGTCAAGGTCTCAACAGCTATAGCCAC TGACACCACCACTGATACGTCTCCTCCGTCTCAAGGAAGGTTCAGGGATGAGTATAAGAGCTTTCTCGAACCAGTGATAG GTTTCTTGGCAAGCAAGCAATCTCCCTTGCTCGTGAATCTCTACCCTTACTTCAGCTACATGGGAGACACGGCCAACATC CATCTAGACTACGCTCTGTTCACCGCCCAGTCCACTGTTGATAACGATCCAGGGTACTCATACCAAAACCTATTCGACGC AAATCTCGACTCGGTTTATGCAGCATTGGAGAAATCAGGGGGCGGATCGTTGGAAATCGTGGTGTCGGAGACCGGTTGGC CCACAGAGGGAGCAGTCGGGACGAGTGTGGAAAACGCAAAGACTTATGTTAACAATTTGATACAACATGTGAAGAATGGA TCACCGAGAAGGCCAGGGAAAGCTATAGAGACTTATATATTCGCTATGTTCGATGAGAATAAGAAGGAACCAACGTATGA GAAGTTTTGGGGACTGTTTCATCCAGATCGACAGTCTAAGTATGAAGTTAATTTCAACTAATCCTTAGAGACTTGTGGGC TTTTTATGTAAGCGTATTTAAAAATTGGGAACTTGTTGTAGTAATAAGGAATAATTAATGCGCTTTCAGCGTGTAGTATG .......................i........................................................ ............................N....N.............................................. ......................i.........N............................................... .....................i.......................................................... ................................................................................ ..................i................................N............................ ............i................................................................... ...............................................i................................ ...........................................................i.................... ................................................................................ .................N.............................................................. .............................................N....................N........N.... .............................................N......N.......................N... .........................................N...................................... .....N...................................................N...................N.. Pos Score Pred ------------------------ 24 0.550 Yes 109 0.362 - 114 0.214 - 162 0.758 Yes 195 0.645 Yes 201 0.370 - 263 0.626 Yes 273 0.441 - 342 0.824 Yes 499 0.520 Yes 532 0.319 - 573 0.718 Yes 688 0.540 Yes 780 0.762 Yes 898 0.319 - 1006 0.120 - 1027 0.148 - 1036 0.280 - 1086 0.307 - 1093 0.099 - 1117 0.141 - 1162 0.313 - 1206 0.167 - 1258 0.052 - 1278 0.052 -

Explain the output -- Processing locus 598: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGAATTTTTCGTGTGGTTAGCGTATGGTTAGGGATGTAACTGATGGATCGTATCCCTATTATTTTCATGAAAAGTTTTGG TTCCTATATATATATATTTAACTCAAAATTATGTTACATATTGTAGAGCTTATGGTTTTGGGATTTATTTCCCTGCTTCT AACATTTGGCCAAAACTACATTGCGAGCATATGTGTTCCATCAAGATACGGCCATGCAATGTCTTTTTGTGGTCCATATG ATGGACCTAGTGAAGATGATCGTAAAAAGCTCAAGAAAACAGACCATGCAATGCGTATTCTCTACTCTGTTCAACGTCGT TCTTTGGCCGACGCTCCTCCAGTAAATTGCAAGAAAGATTATGTCGCACTTATATCATTAAACGCATTACATCAAGTGCA TATATTCATATTCTTCTTGGCCGTGTTCCATGTTATATATAGTGCTATAACCATGATGCTTGGAAGAGCCAAGATTCGTG GCTGGAAAGTATGGGAGCAAGAGGTCATCCATGAACAAGAAATGATGAATGATCCATCAAGATTTAGACTCACACATGAG ACATCATTTGTCCGAGAACATGTCAATTCTTGGGCTAGCAATAAATTCTTCTTCTACGTTATGTGCTTCTTCCGTCAAAT ACTTAGATCAGTGAGGAAGTCTGATTACTTGACAATGCGACATGGATTCATAAGTGTTCATTTGGCACCTGGTATGAAGT TTGATTTTCAAAAGTACATCAAAAGATCTTTGGAAGACGACTTCAAGGTGGTTGTAGGAATAAGACCCGAGCTTTGGGCC TTTGTAATGTTGTTTTTACTCTTCGATGTTCACGGATGGTATGTTACTGCCGTAATCACCATGATTCCTCCTCTATTAAC ACTAGCCATAGGAACAAAGCTACAAGCCATTATATCGTACATGGCATTGGAGATTCAAGAGAGACATGCAGTAATTCAAG GGATGCCAGTTGTCAACGTCTCAGACCAACATTTTTGGTTTGAAAAACCCGATCTAGTACTTCATATGATCCACTTCGTT CTGTTTCAGAATGCTTTTGAGATAACTTATTTTTTTTGGATATGGTATGAGTTTGGGCTAAGGTCCTGTTTTCATCACCA TTTTGGCCTTATAATCATTCGTGTCTGCCTAGGGGTGGGAGTACAATTCCTATGCAGTTATATCACATTGCCCCTTTACG CTCTCGTCACTCAGATGGGATCCACAATGAAGCGATCAGTGTTTGATGAGCAAACTTCAAAAGCATTAGAACAATGGCAT AAGAAGGCGAGGAAAAAGAATGAAAAGTGAGAATAATTGGCTTCTCCTTTTAATCTATGGATAGACCAAATTTAACAGTC TAATAGTTTGGTTACTTAATAGTCTATACACAATTTTTGGAAAATAGGA .......................N.........N........N.......................N............. ..............................i....................N............................ ..............................N......................N....i..................N.. N..............N.............................N....i............................. ........................................N....................................... .............................N......................i..N........................ ..........N...................N..........N..N...N..........................i.... ...................N........................................N................... ..................................i......i...............................N...... ................................................................................ ......i..................N.........i....N...................N................... ........................................i........................N.............. ..N..............................................................N.............. ..........N..............................N....N................................. ...................................................N............................ ..............N...........N..................N...........................N...... ...................N....................................N....................... ................................................. Pos Score Pred ------------------------ 24 0.230 - 34 0.151 - 43 0.258 - 67 0.048 - 111 0.515 Yes 132 0.412 - 191 0.217 - 214 0.285 - 219 0.514 Yes 238 0.171 - 241 0.353 - 256 0.361 - 286 0.344 - 291 0.619 Yes 361 0.123 - 430 0.202 - 453 0.545 Yes 456 0.433 - 491 0.495 - 511 0.346 - 522 0.460 - 525 0.375 - 529 0.153 - 556 0.574 Yes 580 0.359 - 621 0.332 - 675 0.648 Yes 682 0.502 Yes 714 0.172 - 807 0.533 Yes 826 0.153 - 836 0.571 Yes 841 0.092 - 861 0.408 - 921 0.531 Yes 946 0.260 - 963 0.346 - 1026 0.127 - 1051 0.213 - 1082 0.361 - 1087 0.296 - 1172 0.351 - 1215 0.393 - 1227 0.367 - 1246 0.212 - 1274 0.373 - 1300 0.287 - 1337 0.074 -

Explain the output -- Processing locus 599: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCGTTCAATTCTGTTGATTTCACTGTCTAGGTTTCTCCTTTTGATTTAAACTATCTGAACTTGTAACCCTAACCCTAG ATCGGATTTTGTTGTTGATAGATTCTTTATAATCCTAACCTATATATGTCTACCAAAAGAGTAGATATGGGTTCTAGAGA TTATATTAGCAGTCTACCAGATGAGATTCTTGGCAAAATCTTATCATTGTTTTGCACAAATCGTGCTGCTTGCACATCAG TTCTGTCCAAAAGGTGGAGGAATCTGCTCGAGCTTGTAGACAACCTTGATTTGAATGACGCATATGGTAATCATCAATAC TTCTGTGATTTTGTGGACAGAACACTTGCTCTCTTAAGCAATTCTACCACCGTCAAGAGATTCTCCCTAAATTGTGCATT TCACAATGACGCTTCTCGGGTGAACAGTTGGATACGCACTGTCTTCGATCGCGGCTTCTTGGATCTTCACTTGGAGACCG CACATATGCATCGTATAGACACTGAGTTCTTCACGAGCAATACACTTCTTGAGCTCACAATATGCGGTGGGTTTTATGCT GAGGGTCGTCTTCCTCCTGGGAGAGTGTTTTTTCCAGCGCTTAAATCACTCTCTCTAGTTTCAGTGGAGTTTACAGATAC TTTGATGTATCAGAATTTCATCTCTGGCTGCCCTGTGCTTGAGGAATTATTCCTACATTATGATAATGAGACACAATGCC CGGCTTGGAAAGGGCTCGTATCGAGTCCTTCCATCAAACGGCTGAACATCTATGACAATCCTTCCGAGTTACGTTATGAG GCTTACAAATGTTGCTTTCAGATACCCAGCCTTGTGTACCTTGACTATTCTAGTTATGTGGCGAAACAGTATGCGGTTGA TCTGGTTTCCCTTGAAGAAGCTAGACTCAATATCCGATATCCGGAGAGACTTGTAGATCAGAATGAGGATATTTTTGGTG ATGTTACGGATTTGCTTAAAGGGATAAGCAATGTTAAAACCCTTCATTTATCTTCTGATTCTCTTGAGGTGTTTCACTTG TGCTGTGATACAATGCCGGTGTTTCATAACCTCCTTACTTTATCTTTTGAGAGTGACGAAGAAATAGGCTGGCAAGTGGT GCCACTTCTCCTCAACAAATCTCCAAACCTAGAAACTTTAGTCATCAAGAGTCTTGTGCACCAAGTAACAGATATGTGCG GGAACGCCTGCGCTTGCATCTCTAAGAGGAGGAAGAAAAAGAAGAAGACGACGGAGGATGACGAATGTTGTTTATCTACA TGTCAAGTCAAAGTGCTAAATATTTCAGGGTATCGAGGTACTTGTAAAGAGCTGAAACAAATGAGGCATTTCTTGGAGAA TTTGAAATGTCTTGAAACTGTCAATGTGGGTGTTGATGTGAAGCGCAGAGAATATAAGAATGGTAATAACAAGTACCAGA GGATCACCAATGCTGTTATGGAGCTTCCTAGATTTTCATCAAACTGTCAGATCCAATTCTTCTGATTTCTCATTTGAAAA CTGAATCATTAACATGCCAAAACTAAATCTTTTAGAAGATAAACTTTTTTTCTCTACGAAGGTAATCAATATTTTGCTAT TTAT ................................................................................ .............................................i....................i............. ....................N........................................................... ......................................................i........N................ ................................................................................ .....N.......................................................................... .....i.......................................................i.............N.... ................................................................................ ....N......................................................N.....N.........N.... ...................................................N.......................N.... ........i..............................................N..............N......... ..............................................................N................. N.............................N................................................. ............i................................................................... .........................................................................i...... .........................................................N......N..............i ............................................................i................... ......N................N...........N.......................N.................... .........N.......N.............................................................. .............N.................................................................. .... Pos Score Pred ------------------------ 126 0.664 Yes 147 0.604 Yes 181 0.331 - 295 0.509 Yes 304 0.361 - 406 0.479 - 486 0.529 Yes 542 0.549 Yes 556 0.122 - 645 0.421 - 700 0.178 - 706 0.232 - 716 0.307 - 772 0.336 - 796 0.248 - 809 0.569 Yes 856 0.147 - 871 0.216 - 943 0.168 - 961 0.120 - 991 0.129 - 1053 0.812 Yes 1194 0.648 Yes 1258 0.358 - 1265 0.354 - 1280 0.529 Yes 1341 0.793 Yes 1367 0.494 - 1384 0.356 - 1396 0.187 - 1420 0.354 - 1450 0.180 - 1458 0.290 - 1534 0.163 -

Explain the output -- Processing locus 600: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATGAACAAAAACAGTCATATATAGATAGCTAATTTAGGATCCAGTTATAATAATCATGTTTGGTCCACCATGTGGACG CACGCTATTATCCAAATGTGACTTACAAGCGAGAAACCCACTAGCACTCACATGCACAGTCGACTTATTCATATATATGT TGCTTTTTATCACATAAAGCTCGTTGTGCAAAAGAACAGAATGAGTTCTAAGTTTGATATCGGCTCCTTAAGCATAGTAA TGACTCTGCTTATTTGCTTCTTATTATTATCTTTGGCTCCAAAACTTGAGGCTGAGCTCGCAACGGTCCAACATGCTCCA AACCCCGATGGCTCTATCAGTTTTCTGGTGATTGGAGATTGGGGTAGGCATGGACTCTACAACCAATCCCAAGTTGCCCT CCAGATGGGGAGAATCGGGGAGGAGATGGATATCAATTTTGTGGTATCGACGGGTGATAACATCTACGATAACGGGATGA AAAGTATCGATGATCCCGCCTTTCAACTCTCCTTTAGTAATATCTACACTTCTCCTAGCCTGCAAAAGCCTTGGTACCTT GTATTGGGGAATCACGACTATAGAGGAGATGTTGAGGCACAATTGAGTCCTATCCTCAGATCTATGGATAGCCGTTGGAT TTGCATGAGATCCTTCATCGTTGACGCTGAGATCGCAGAGCTCTTCTTTGTCGACACAACTCCTTTTGTAGATGCTTATT TCCTCAGTCCACAAGACCAAACTTACGACTGGAGCGGCGTATCACCGCGAAAATCCTACCTTCAGACCATCTTAACGGAG TTGGAGATGGGTCTAAGAGAATCAAGTGCAAAATGGAAAATTGTGGTGGGTCACCATGCTATAAAGAGTGCTTCCATTCA TGGGAATACCAAAGAGCTTGAATCTCTCCTCTTACCCATCCTTGAGGCGAATAAAGTTGATCTCTATATGAACGGTCATG ATCATTGCTTGCAACATATAAGCACATCACAAAGCCCAATTCAGTTTCTAACGAGTGGTGGTGGGTCAAAGGCATGGAGA GGCTATTACAATTGGACAACACCAGAGGATATGAAATTTTTCTACGATGGACAAGGTTTCATGTCAGTTAAGATCACTAG ATCAGAATTGAGTGTCGTTTTTTATGATGTCTCCGGCAATAGTTTGCATAAGTGGGACACATCGAAAATGTTAGACTCGG ATTTTTATTTCCCATTGTAATTCACATGAAAATCAGTTAAAGCACATAAATTCTACATATAAATAAATATGATTACTATG AGTGGCTAAAAAAAAATCAAGTAAATTAGGCAAACTCAG ..N......................................................N.............i........ ...............i...................................N........................N... ........................................i......................................i ........................................................................i....... .......N.........................................N.............................. ....i....................N..................................................N... .........N...................................................................... ............................i..................................N................ ....i..................................................................N........ ................................................................................ ......i.........................i......................N.......................N ...................................................................i.........i.. .........................................................................i...... ..............................i...............N.............i................... .......................N..N........................................N............ .........................N..........................................N........N.. ....................................... Pos Score Pred ------------------------ 3 0.348 - 58 0.322 - 72 0.506 Yes 96 0.502 Yes 132 0.398 - 157 0.170 - 201 0.739 Yes 240 0.728 Yes 313 0.565 Yes 328 0.311 - 370 0.164 - 405 0.722 Yes 426 0.467 - 477 0.322 - 490 0.221 - 589 0.539 Yes 624 0.341 - 645 0.561 Yes 712 0.229 - 807 0.643 Yes 833 0.640 Yes 856 0.164 - 880 0.215 - 948 0.529 Yes 958 0.544 Yes 1034 0.560 Yes 1071 0.517 Yes 1087 0.178 - 1101 0.565 Yes 1144 0.432 - 1147 0.432 - 1188 0.363 - 1226 0.138 - 1269 0.062 - 1278 0.146 -

Explain the output -- Processing locus 601: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACATAATTCATTTGTGGAGCACTATAATTATAGACGGTGTCAAGTAACGGCGTTAGCACCGACGAGGAAGGCGATGGTAT AAAAGGCACACCCCAACATAAATGACAATAGTATCATCATTCTCACAACAACACAACACACGCAGAAGAGAGAGAGAGAA CAAAAAAAAGAAGGAGCGGTTACATATTCTCTGGTCTGTCACAAGAAAAATGGATATCTGTATGGACAAGGAGCCAGATG GTGTGGTGGTCTATGCGAACGGTGATTCGTGTAATCCAAACCAAGAAAATGTTTCTGAGCCTCTCTTAGATTCAGTATCA CGCGATGATGCTAATGTACATACAGAGCTTCGATATGGGGAAGAGAACATCGAAGTAAACGAATATGATGTTAAAGAGTG TACTAGTGAGATCCCCGTTGGTAAGCCGATAGGAGATGATTTTGAGTCTAAAGATGTCACTAAGTCAAGTTTACATGCAA AGCATGCTTCAAAATCAGGTAGAGGGAATAACAAAACAAGGAACACCGTTCCACAACCGTTTTCTTTAGCAACTGAGAAA CGTGCTTCATCTACACGTTCGTTTACTAGTGAATCTCTCGAATCAGCTGGCTTGAAAAAGTTTCCGGATGGACATAGTAA AGTACAAAGTCAGGCAACAAAGGTACCTAGGAAACCATTGCAGCCAAAAAACAAGAAGCTTTCAGATGAGGAAGATTCTT GCTCTGTTGCTTCATATGCCACGTCGGGTGCAAAGTCTGCTAAATCAAGAACTGTTGTCACCGCAGCTCCTTCGTTCCGA TCCACTGAGCGTGCCGAGAAGAGGAAGGAGTTCTATACAAAACTGGAGGAGAAACACCAAGCCATGGAGGCCGAGAAAAC GCAAAGTGAAGCAAGGAACAAGGAAGCAACCGAAGCAGCTTTAAGACAATTAAGAAAAAGTTTGAGGTTTAAAGCAAACC CAATGCCAAAATTTTATCATGAAGGGCCTCCCCCAAAGGTTGAACTTAAGAAGCCTCTTCCAACTCGAGCAAAGTCGCCA AAACTAGGGCGGAGGAACCCGAAAGAAGGAAACAGAGCAAAAGGAGCTTCGAGGCGGCATGAAACTCGAAAGACTCTAGT GATCTCTAAAGAAGACCATGATGATGAAACCACACGGAATGCTGACCAGATAAACCATAAAGAAATGAACCGAAATTTGG AACCAGAGACTGCTTTTGCCTGCTAAAGAGTCTTTTTGGTCAGAACAAACTGAGAGTTATGTTTTTGTTGTAAGAATGTG TGGGATGCTTGTGTTTTTAACTTATGATTTGAAGTGTAAATGTGT .........................................................................N...... .....................N.......................................................... .................................................i...........N...............N.. ............N...................................N............................... ....N..N.....N....................N.............................N..N............ ...................................N.................N....................N..... ...N............................................................................ ...................................................................N............ .................................................................i.............. ...............N................................................................ ...............................................................i................ ................................................................................ ..i...............i............................................................. ..........................................................N..................... .................N..N..N..............N.........................N............... ..........................................................N................N.... ....N..................N...............N..... Pos Score Pred ------------------------ 74 0.277 - 102 0.312 - 210 0.689 Yes 222 0.472 - 238 0.131 - 253 0.201 - 289 0.249 - 325 0.118 - 328 0.417 - 334 0.217 - 355 0.172 - 385 0.231 - 388 0.225 - 436 0.185 - 454 0.165 - 475 0.129 - 484 0.105 - 628 0.276 - 706 0.607 Yes 736 0.121 - 864 0.576 Yes 963 0.510 Yes 979 0.715 Yes 1099 0.110 - 1138 0.214 - 1141 0.215 - 1144 0.322 - 1159 0.232 - 1185 0.429 - 1259 0.057 - 1276 0.068 - 1285 0.045 - 1304 0.060 - 1320 0.049 -

Explain the output -- Processing locus 602: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGATGATGATATATATAACATCAACGGTTTTTGAAGAGTAGGTGCAAATTCTTTTTGTGTATCATCATGTAACTCTTTGA CTCTCAGTTTAAAGTCAATAAAATATATCGCTGCCTAATTATATCAATGAGCAGTTGAAATCAATTCTTGCTAGATTTGT GTTCAAACAAACTATGTTCTTGTTCTCTTCACGTAGAATCACCTCTCTACGCTCTTACACCATAATCAAACATTCAAGTT GTTATTCGACTTTGGTTTCTGATGGTAACATCTTCTCGATTCAACACTTTCAATCTCTTATGCAAAAATATGAATCAAAC CTTAAAATCATCCACCAGCTCCATTCACACTTCACCACTTCTGGGTTTCTTCTCCTTCATCAGAAGCAAAACTCTGGGAA ACTTTTTCTATTCAATCCTCTGCTTCGATGCTATTCTCTTGGCGAAACCCCTCTTCATGCTTACTTCCTCTACGACCAAC TCCAACGACTCCATTTCTTGTCTGATCACAATAAGAGTCTGCCTCCTTTTGATAGTTTCACTTACCTCTTTCTCCTGAAA GCGAGTTCGAATCCGCGGTTCCCATCTTTACTACTGGGAATTGGACTCCACGGGTTGACTCTAAAACTGGGTTTTGAATC TCATGTCTACGTGCAGACAGCTTTGGTTGGTATGTATCTTGTTGGTGGAAACATGATTGATGCTCACAAAGTGTTTGATG AAATGCCTGAGAGAAATCCGGTAACTTGGAATGTGATGATCACTGGCCTTACTAATTTGGGAGATTTCGAAAAAGCTCTT TGCTTTTTGGAGAAGATGCCGAATCGGACGGTAGTGTCTTGGACCACTATCATTGATGGGTATGCACGAGTAGATAAACC CAAGGAAGCTATACTCTTGTTTTCAAGAATGGTTGCTTGCGACGCAATAAAGCCCAACGAGATAACCATTCTCGCCATTC TCCCAGCTGTTTGGAACTTGGGAGACTTAAAGATGTGCGGATCAGTTCATGCTTATGTTGGGAAAAGAGGGTTTGTTCCA TGTGATATCCGTGTGACTAACTCTCTCATCGACGCTTATGCAAAATGTGGGTGTATTCAAAGCGCGTTCAAGTTTTTCAT AGAGATTCCAAACGGAAGGAAGAATCTTGTATCGTGGACAACGATGATCTCGGCGTTTGCAATTCACGGAATGGGAAAAG AAGCGGTCAGTATGTTTAAAGACATGGAAAGATTAGGATTGAAGCCGAACAGAGTGACCATGATCAGTGTTTTGAACGCT TGCAGCCATGGAGGTTTAGCAGAAGAAGAGTTTCTAGAGTTTTTCAACACGATGGTAAACGAGTACAAGATCACGCCGGA TGTGAAGCACTATGGATGTCTAGTAGATATGTTGAGAAGAAAAGGAAGATTAGAAGAAGCAGAGAAGATTGCTTTGGAGA TTCCAATAGAAGAAAAAGCTGTGGTTTGGAGAATGCTGCTTGGAGCTTGTAGCGTTTATGATGATGCTGAATTGGCTGAG AGGGTTACTAGAAAGCTAATGGAGCTAGAGAGAAGCCATGGAGGAGACTATGTGCTTATGTCCAACATCTTTTGTGGTAC TGGAAGATTTTTAGATGCGCAGAGATTTCGGAAACAGATGGATGTTAGAGGAGTAGCTAAACTTCCAGGACATTCACAAG TAACATAGCGGAGCATGTATATATTTATGACTCCAATCTTTATTGTTTTGATGATAAACTTCAGAATTTTTGGGAGCTCT GTTCTGATCCTCAATGTTTTGATCATA ..N..N............................................................N............. ..............................................N................................. .............N.................................................................. .....................N.....................................i.........N.......... ................................................................................ ...........................N............................N....................... ................................................................................ ................................................................................ ..N............................N....................i......N.................N.. ..i...........................N....N............................................ ...............N.......................................N.....N.................. ............................i................................................... ................................i...............N.....N........................N .....................................N......i................................... ...........................................N..........................N......... ...........N...........N...................................N.................... .......N...........................................i...........................N ...........N...i............N................................................... ................................N........................N..N..N................ ..................N..................i...........N.......N...................... ..............N......................N...N...................................... ..............N...........N.......................N............................. .............N............. Pos Score Pred ------------------------ 3 0.234 - 6 0.306 - 67 0.082 - 127 0.213 - 174 0.142 - 262 0.300 - 300 0.638 Yes 310 0.459 - 428 0.327 - 457 0.260 - 643 0.123 - 672 0.397 - 693 0.748 Yes 700 0.268 - 718 0.068 - 723 0.676 Yes 751 0.127 - 756 0.298 - 816 0.328 - 856 0.440 - 862 0.108 - 909 0.618 Yes 993 0.788 Yes 1009 0.261 - 1015 0.261 - 1040 0.461 - 1078 0.117 - 1085 0.753 Yes 1164 0.415 - 1191 0.423 - 1212 0.437 - 1224 0.296 - 1260 0.388 - 1288 0.120 - 1332 0.552 Yes 1360 0.184 - 1372 0.232 - 1376 0.582 Yes 1389 0.340 - 1473 0.401 - 1498 0.253 - 1501 0.309 - 1504 0.085 - 1539 0.333 - 1558 0.632 Yes 1570 0.382 - 1578 0.469 - 1615 0.365 - 1638 0.291 - 1642 0.259 - 1695 0.166 - 1707 0.105 - 1731 0.041 - 1774 0.036 -

Explain the output -- Processing locus 603: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCGTGATAGTTTTTAATTGCACAATGTGTTTGTTTGTTGGGTTTTTAGCTAAATCTGCTAATAAAGTCTGAATTTTTT TGTGGGTATATAAGGCTCTAAGCATTTATGTATGTATACAATTTTGTTGTTGTTGTAGTAGTGGTAATGGGGAGAGAACT TGTAGACAAACACATGGATAAGAAAGCGAATAGTTTGACAGCTAGCTCGACGGGATCTTCAGATGATAATAAAGTCCCAA GTCCGTCTACTAATGAAGCTGCAGAGGTGAAAGAATGCACCGAGCAGAATTTGGTTGCGGATGATGCTCGTTTGAGGCAA CAAGGGATAACTGAGACACCTGGATCCCATAAATCTTCTGTTAAACCTCGTGTTACTGCGAAAACAACTGTTCCAAAGCC GTTTTCCCTGTCGGCTGAAAAGCCTAGAAGAGCAGCGGTTGATAATAATTCATTAGGAAATGGTGCTAGCCATAACTCTT CTTCAGCATCAAGAGTTTCACAGCTAAACTCTCCTTTACCAACTAGGAGGATACCAGATCATAAGATGCACCATGATGAA GAAGATAGTTTTTCTGTTGCTTCCTCATCAGCTACATCCATAAGAAGTTTTAAACCCAAGATAACGATTGGAGTTGCTCC AACATTTAGTAGCACATCGCGACTGGAGAGACGAAGAGAGTTTTACCAGAAGTTAGAGGAAAAACAGAAAGCACTTGAAG CAGAGAAAAGAGAAAACGAGAAAAGGCTTAAGGAAGAGCAAGAAGCAGTCACTAAGCAACTTAGGAAGAACATGGCTTAC AAAGCTAATCCGGTACCAAGTTTCTACCAAGAGGGTCCTCCACCGAAACAACCATTGAAAAAGTTTCCATTGACTCGGCC TAAGTCACCAAACCTTAACCGTAGAAAGAGCTGCAGCGACACAGTTAACGCATCATATCAGGAAGTGAAGGGGAAGCATT GTGCTCGACATCGTCACAGTGTAGGTGGTTGCAAGGATGAAGTCAAAACGAACAGTGTCCCAAGGACACCAAATAGCAGC AGCAAAGATCAGATGAGAAAGTCCAAAAAGGGAACACCAAAGTCTGAAGAAGTACATGAAATGTTTAATAGTGGCCATGA TGGAGAGACTGGTGAGAATGGCGTTGGTGTTGTGGAGGAGTGAAGCTTCGCGTAGTTGTAGTTTCGTTTTGACTAACTTT GGTGAATGAGAAAAACAAGAAAAATGGTGAGAAGCAACTGCAAGGAGAAGAACAGGTTTTAG .........................N...................................................... ...........................i...N..................................i............. .............i................................................N................. ............N.....................N.........................N..N................ ................................................................................ ...........................................................N.................... .................................................................N......N..N.... ................................................................................ ................................................................................ .......................................................................N........ ................................................................................ ................................................................................ ....................................N........................................... ............i..........................................N....N...............N..N .................N.............................................................. .....N.................N...................................... Pos Score Pred ------------------------ 26 0.218 - 108 0.701 Yes 112 0.166 - 147 0.736 Yes 174 0.800 Yes 223 0.205 - 253 0.145 - 275 0.454 - 301 0.214 - 304 0.110 - 460 0.471 - 546 0.344 - 553 0.374 - 556 0.369 - 792 0.340 - 997 0.316 - 1053 0.551 Yes 1096 0.161 - 1101 0.389 - 1117 0.273 - 1120 0.208 - 1138 0.364 - 1206 0.050 - 1224 0.178 -

Explain the output -- Processing locus 604: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCTTTATATAGTTCTCTCTCTGTTCCTCATATTTCTTCATCGTTTTGTTTCGCTTCTCTTTCTCTGTCTCTCGTATCT TTCTACTACTCTGTTTCTTGAATTCTAATGAACAACATCGACGACGCAAAGACGGAGACTTCAGTGTCTTCAGGTTCAAG CGACTCTTTCTTGCCTCTCAAGAAACGCATGAGACTTGATGACGAACCAGAAAACGCCCTAGTGGTTTCGTCTTCACCAA AGACGGTTGTGGCTTCTGGCAATGTCAAGTACAAAGGAGTCGTTCAGCAACAGAACGGTCATTGGGGTGCCCAGATTTAC GCAGACCACAAAAGGATTTGGCTTGGAACTTTCAAATCCGCTGATGAAGCCGCCACGGCTTACGATAGTGCATCTATCAA ACTCCGAAGCTTTGACGCTAACTCGCACCGGAACTTCCCTTGGTCTACAATCACTCTCAACGAACCAGACTTTCAAAATT GCTACACAACAGAGACTGTGTTGAACATGATCAGAGACGGTTCGTACCAACACAAATTCAGAGATTTTCTCAGAATCAGA TCTCAGATTGTTGCGAGTATCAACATCGGGGGACCAAAACAAGCCCGAGGAGAAGTGAATCAAGAATCAGACAAGTGTTT TTCTTGCACACAGCTTTTTCAGAAGGAATTGACACCGAGCGATGTAGGGAAACTAAATAGGCTTGTGATACCTAAAAAGT ATGCAGTGAAGTATATGCCTTTCATAAGCGCTGATCAAAGCGAGAAAGAAGAGGGTGAAATAGTAGGATCTGTGGAAGAT GTGGAGGTTGTGTTTTACGACAGAGCAATGAGACAATGGAAGTTTAGGTATTGTTACTGGAAAAGTAGCCAGAGCTTTGT CTTCACCAGAGGATGGAATAGTTTCGTGAAGGAGAAGAATCTCAAGGAGAAGGATGTTATTGCCTTCTACACTTGCGATG TCCCGAACAATGTGAAGACATTAGAAGGTCAAAGAAAGAACTTCTTGATGATCGATGTTCATTGCTTTTCAGACAACGGT TCCGTGGTAGCTGAGGAAGTAAGTATGACGGTTCATGACAGTTCAGTGCAAGTAAAGAAAACAGAAAACTTGGTTAGCTC CATGTTAGAAGATAAAGAAACCAAATCAGAGGAGAACAAAGGAGGGTTTATGCTGTTTGGTGTAAGGATCGAATGTCCTT AGGGAATTTTTCTTTAAAAGTTTCTTACTTCAACTAGAACTTGTTTTACTTGTACCTCATGAATTTTTATTAACAAAATT GTTTTACTTAACTAGAGATTG ................................................................................ ...........................i.................................................... ............................i.........N......................................... .....................i.......................................................... ...........................................N.................................... ................................................................................ ..........................i..................................................... ................................................................................ .........................................i...................................... N.............i...............................................................N. ...........................N.......i............................................ ............N........................................N.......................N.. .........N.....................................i......N......................... ........................i.........N............................................. ..........................................................N..................... ..................... Pos Score Pred ------------------------ 108 0.587 Yes 189 0.736 Yes 199 0.273 - 262 0.635 Yes 364 0.397 - 507 0.622 Yes 682 0.529 Yes 721 0.210 - 735 0.662 Yes 799 0.337 - 828 0.262 - 836 0.594 Yes 893 0.171 - 934 0.312 - 958 0.229 - 970 0.273 - 1008 0.588 Yes 1015 0.155 - 1065 0.640 Yes 1075 0.157 - 1122 0.159 - 1170 0.114 - 1193 0.171 - 1259 0.046 -

Explain the output -- Processing locus 605: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTAAAATTGTAACACTGTAAAACCATCAAAATCCTATTAGAAAAAAAAGAATTACCTTACTCGCATCCTCTATAAATAC TCTGCTTTGGCCTTTGGGTAACAAATCAACAAATCAACTCTGAAAACAATTTACGAAGAAGAAAGAAACCCTAAAAATAT GTTAATGGAAGAAGAGACCTGCTTGGACTGCAAGAGACCAACCATAATGGTGGTTGATCACAGTAGCGGTGACACCATCT GTTCGGAGTGTGGCCTTGTCCTCGAAGCTCACATCATCGAATACTCTCAGGAATGGAGAACTTTCGCATCCGACGACAAT CACAGTGACAGAGACCCAAACCGTGTTGGTGCTGCGACAAACCCTTTTCTCAAATCAGGCGATCTTGTCACCATCATCGA GAAACCTAAAGAAACCGCTTCCTCTGTTCTGTCCAAAGATGACATCTCCACTTTGTTCCGTGCACATAATCAAGTCAAGA ACCATGAAGAAGACCTAATCAAGCAAGCCTTCGAAGAAATACAGAGAATGACCGATGCACTAGACCTAGATATCGTAATC AACTCTAGGGCTTGTGAGATTGTCTCCAAATATGATGGTCACGCTAACACCAAATTACGTAGAGGGAAGAAGCTAAACGC TATATGTGCAGCCTCTGTTTCAACGGCTTGTCGTGAACTGCAACTCTCGAGGACATTGAAAGAGATCGCTGAGGTTGCCA ATGGTGTGGACAAGAAGGATATCCGGAAAGAGAGTCTGGTGATAAAGCGTGTTCTTGAGTCACATCAAACCTCCGTGTCT GCTTCTCAAGCGATTATCAACACCGGAGAACTCGTTCGCCGGTTCTGTTCCAAACTTGATATAAGTCAAAGAGAGATAAT GGCGATTCCAGAAGCGGTTGAGAAAGCAGAGAATTTCGATATCCGGAGAAACCCTAAGTCAGTTCTTGCAGCAATTATCT TCATGATCTCTCATATATCTCAGACCAACAGGAAACCTATTAGAGAAATTGGCATCGTTGCGGAAGTTGTGGAGAATACG ATCAAGAACTCGGTTAAAGATATGTACCCGTACGCCTTGAAGATTATACCTAACTGGTATGCTTGTGAATCAGACATCAT CAAAAGGTTAGATGGTGTCATCACGTCCTGGGATTCTGCAAAATTTTCAGTATAAGGAAGTCACTACCTGTGAAAAGAAG AAGCTTTGGGATTGTTGTAGTCTCGTTTCTGATGCATTACATACATATATGTAAAGTCGTTGGCTTTATGGAAG ................................................................................ ..............................................................................i. ....i.........................................i................................. ....................................................N........................... ................................................................................ ......................................N......................................... ...N...........................................i......i......................... ...............................i..N............................................. ...N............................................................................ N............................................................................... ..............................................................................i. ................................................................................ ..N............................................................................. .....................N....................................N..................... ...........i.................................................................... ...............................N................N..................N...... Pos Score Pred ------------------------ 159 0.607 Yes 165 0.775 Yes 207 0.706 Yes 293 0.265 - 439 0.488 - 484 0.315 - 528 0.784 Yes 535 0.533 Yes 592 0.600 Yes 595 0.411 - 644 0.385 - 721 0.313 - 879 0.745 Yes 963 0.488 - 1062 0.314 - 1099 0.423 - 1132 0.507 Yes 1232 0.041 - 1249 0.051 - 1268 0.046 -

Explain the output -- Processing locus 606: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTTTATCTGTGACATTTATTCTTCTTGCGAGCAACATCGACTGATTCTTACCTGTATGTTTCGATTTCACATAGACACA CGAGAGAACGCTAATTTCTAGTCCTCTGTTTTCGTGGATCAGTCTGTTTTTTCTATGCAATTGTGTGTTCTTGATGTTGT TGACACAATGATTTCAGGTTATAGCAAGGAGCTCGTGATCATGAGTGGTACAGAGTTAGATGAGCCTGGAAAGTTAAAAC GTGCTGTCATTGATGCTTCTGCTGGTGGTGTTGCTGGTGCGATTTCACGTATGGTTACCTCTCCCCTTGATGTTATCAAG ATTAGATTTCAGGTTCAGTTGGAGCCTACAGCTACATGGGCTCTGAAAGATTCTCAGCTGAAACCGAAGTACAATGGTTT GTTTCGAACAACGAAGGATATCTTTAGAGAGGAAGGTTTATCGGGTTTCTGGCGTGGAAATGTTCCAGCTTTACTTATGG TTGTGCCTTATACATCCATACAATTTGCAGTCTTACATAAGGTGAAGTCTTTTGCCGCTGGTTCATCAAAGGCCGAAAAT CATGCTCAACTGAGCCCTTATCTTTCTTACATCAGTGGGGCACTAGCAGGGTGTGCAGCAACAGTTGGATCGTATCCGTT TGACCTATTGCGGACTGTATTGGCGTCCCAGGGTGAGCCAAAGGTGTATCCAAATATGAGGTCAGCATTTCTGAGTATAG TGCAAACTCGTGGAATCAAAGGGTTGTATGCAGGATTGTCCCCTACTCTGATCGAGATCATTCCTTATGCTGGTCTGCAG TTTGGCACCTATGACACATTTAAACGCTGGAGTATGGTATATAATAAGCGATACCGATCTTCTTCATCAAGCTCAACGAA TCCAAGTGATAGTCTTTCTAGCTTTCAGCTTTTCCTTTGCGGTTTAGCTTCGGGCACTGTTTCTAAACTAGTCTGTCATC CGCTTGATGTTGTCAAGAAAAGATTCCAGGTCGAAGGTCTGCAACGTCACCCAAAATATGGAGCCCGAGTAGAGCTGAAT GCTTACAAGAACATGTTTGACGGTTTAGGGCAGATATTAAGATCTGAAGGTTGGCACGGGCTTTATAAAGGCATTGTTCC ATCAACCATTAAAGCTGCACCTGCTGGTGCTGTAACATTTGTGGCCTATGAACTTGCATCCGATTGGTTTGAGGCAAATT TGACCTGAACTTGTAAAATTCTTCAATTCTATTTGTAAATCTTATTCTATTTTGCTTTTGAGTAATAAGTAATATAAGAT TCCATCCTTCTCTGAACAGAGACCAAT ........................................................N....................... ......................................................i..................N...... .......i................................i..................N.................... ............N.....................................N..................N.......... ...................................i.....................................N...... ...........................................................N................i... ................................................................................ .......................................................N........................ ...........................N......................................N............. ..........N......................N.............................................. ................................................................................ ......N..................................................i....................N. ............i................................................................... ...............................................N................................ ................................................................................ ........................... Pos Score Pred ------------------------ 57 0.133 - 135 0.637 Yes 154 0.108 - 168 0.552 Yes 201 0.721 Yes 220 0.118 - 253 0.067 - 291 0.438 - 310 0.075 - 356 0.646 Yes 394 0.310 - 460 0.092 - 477 0.573 Yes 562 0.257 - 696 0.420 - 748 0.266 - 787 0.294 - 811 0.232 - 834 0.458 - 967 0.213 - 1018 0.593 Yes 1039 0.420 - 1053 0.587 Yes 1168 0.115 -

Explain the output -- Processing locus 607: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCCACGTTTACCAACACGTGTATCCTCCTCCGACCATGTCATGTTCCTCAACCTGCCACATGTCAGATCCTATGTTGAGA TATATTGCTACTTCAATGTAGCATGTTGTATATATGGCCATGAATGTGTTGTGTCGAGTGTTCACTTCGTCAAGAGTCTT ATCATCAAACGTTACGTTCTCGTTTTCTCAAATTCCAAACAATACCAAGAAACCTCTACTTGAAAAGAGAAGATTATCGC CGCGTGTGTGCCTAAGAGCGATGGCGTCTGAGAAACAAAAACAACATGCACAACCTGGCAAAGAACATGTCATGGAATCA AGCCCACAATTCTCTAGCTCAGATTACCAACCTTCCAACAAGCTTCGTGGTAAGGTGGCGTTGATAACTGGTGGAGACTC TGGGATTGGTCGAGCCGTGGGATACTGTTTTGCATCCGAAGGAGCTACTGTGGCTTTCACTTACGTGAAGGGTCAAGAAG AAAAAGATGCACAAGAGACCCTACAAATGTTGAAGGAGGTCAAAACCTCGGACTCCAAGGAACCTATCGCCATTCCAACG GATTTAGGATTTGACGAAAACTGCAAAAGGGTCGTTGATGAGGTCGTTAATGCTTTTGGCCGCATCGATGTTTTGATCAA TAACGCAGCAGAGCAGTACGAGAGCAGCACAATCGAAGAGATTGATGAGCCTAGGCTTGAGCGAGTCTTCCGTACAAACA TCTTTTCTTACTTCTTTCTCACAAGGCATGCGTTGAAGCATATGAAGGAAGGAAGCAGCATTATCAACACCACTTCGGTG AATGCCTACAAGGGAAACGCTTCACTTCTCGACTACACCGCTACAAAAGGAGCGATTGTGGCGTTTACTCGAGGACTTGC ACTTCAGCTAGCTGAGAAAGGAATCCGTGTCAATGGTGTGGCTCCTGGTCCAATATGGACACCCCTTATCCCAGCATCAT TCAATGAGGAGAAGATTAAGAATTTTGGGTCTGAGGTTCCGATGAAAAGAGCGGGTCAGCCAATTGAAGTGGCACCATCC TATGTTTTCTTGGCGTGTAACCACTGCTCTTCTTACTTCACTGGTCAAGTTCTTCACCCTAATGGAGGAGCTGTGGTAAA TGCGTAAGCGTGGAGTGGACAAGACCGGTCTTAACGTCTTAGACCATTAAATATGATATGATGATGTTGTTTGAGTTTAG GGGCTTTTTGTTATGTTGGTAATGTG ...................................i....N..................i...........N........ ...............N......i..........N.....N...N.................................... ................................................................................ ....................i........................N....................i....i........ ................................................................................ ................................................................................ ......N...................i..................................................... .....................................N...........N.................N............ ............................................N................................... ...........................N.............i...................................... ................................N.....................i......................... ...N.....................................N...................................... ....................................................N....N..N..N................ ............N........N.... Pos Score Pred ------------------------ 36 0.522 Yes 41 0.319 - 60 0.607 Yes 72 0.321 - 96 0.327 - 103 0.500 Yes 114 0.485 - 120 0.405 - 124 0.189 - 261 0.788 Yes 286 0.428 - 307 0.506 Yes 312 0.645 Yes 487 0.371 - 507 0.858 Yes 598 0.297 - 610 0.247 - 628 0.110 - 685 0.201 - 748 0.299 - 762 0.779 Yes 802 0.360 - 913 0.214 - 935 0.547 Yes 964 0.330 - 1002 0.157 - 1042 0.560 Yes 1102 0.780 Yes 1120 0.565 Yes 1173 0.065 - 1178 0.029 - 1181 0.048 - 1184 0.042 - 1213 0.038 - 1222 0.035 -

Explain the output -- Processing locus 608: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAATGGATTAATAATGGTCATTAGTGGAAACGTGTAGTGTTTAAATCCGTGACATGCGTCAAAAGTTTCTTCCTTATTA ATAAGACGGCACGACGTTTAGTCATATTCCAACCCGTTTAAAACGGCACCACGTTTTTTCACCGTCCTTTCATATTTCTT CTCTTATTCCTTAAGTTTTTCTCACTGTGAAGTCAAATCGATGCCTTTCGTTCTTATCACCGCCATGGAGAAACAGAGAT CGAAGAAGACGAAGATATCCGATGATCTAATTACATGCAGTGGAAACTCGGTTCAGATTCCGTTTGATCTCATCCCGGAG ATACTCAAAAGGTTACCTGTCAAAACCCTAGCTAGGTTCCTCTCCGTATCAAAAGAATATACTTCCATCATTCGCAACAG AGACTTCATGAAATCGTACTTGATTAATTCTTCGACTCGCCCACAAAGTCTCATCTTTACAATCGCAGGCGGCGGAATCC ATTGCTTCTTCTCCTTGATTGATCAAGGTGAATCAACTTCGTCTTCTAAACCAACTTACCTTATGAATTGCCCTCATCTA CAGCTTAAAACCTTTGCTCCTTCTGTTCACGGTTTGATTTGCCATGGACCTCCTTCTACACTAATAGTGTCTAGCCCTAG ACTAATAGTATCTAACCCTAGCACTAGACGATCCATTATCCTGCCCAAGATTGATGCTAATCATGAATGTATATACCACC ATATGGGTTATGATCCTATTGATGGTGATTACAAAGTGTTGTGTATGATGAAAGGCATGCATGTTTATCAGAGACGGTAT TTAGCTAAGGAGCTTCAGGTTTTCACTTTGAGAAAAGGGAATTCTTGGAGGATGGTTGAAGATTTTCCTCCTCACTGTCT CTGTCATGAAGATACTCCTGATTTGTGTATCAATGGTGTTTTGTATTATGTAGCTATGCTCGACACAGCTTCGAATCATG CAGTCATGAGCTTTGATGTTAGATCTGAAAAGTTTGATCTTATTAAAGGAGGACCAGACGGGGACTTGAATCCAAAGCTG ACACGCTACGAGGGAAAGCCTGCACTCCTGTTTCCTGGATCTGATTATAGAATTAACTTGTGGGTTATAGAGGATGCTGC AAAACACGAATGGTCCAAGATGTCTTATGATGTTTCGTCTACTTCACTAATTCGCAATCCTTACTTTCATCATTGTGTCT TTTGTACTAATGATGCAGGGGAGATTGTTTTGGCACCCGACTTTGTGCGTACAAAAACATTTGTTGTTCTTTATTATCAT CCAAAGAAAAATACTATGAGAAGTGTCGTCATCAAAGGAATCAGAGATCGAAAGATTCCACTTTGGGATGAGGCTTCATA CCATAGGATAATCTCGGTCTTCTCTGGCCAGGTTGATAATCTCATGTTTCTCTAAGAAATGTTTGTTCTTCATCTTTAGA TGTGTTTCATCGTACTTGCTTGGGAGGTTTTTTGACTATCTTTTGTCCTTTGGTTGGTGACAGTTTGTTTATGG ...i..........i.......................................N......................... ................................................................................ ........................................i.......................N............... .....................N............i............................................. ................................................................................ .......N........................................................................ ..............................................................N................. ...........................................N.................................... .....................................................N........N...i............. ..N......N...........N......................N..N........N...N................... ...................................................i............................ .....N..........................N..............N.......i.....................N.. .....i.........N................................................................ .........................................................................N...... .........N.........i......N..N.................................................. .........N..N................................................................... ...............i...................................................N............ ...........................................N..............N....................N ......................................................................N... Pos Score Pred ------------------------ 4 0.564 Yes 15 0.639 Yes 55 0.244 - 201 0.743 Yes 225 0.355 - 262 0.137 - 275 0.560 Yes 408 0.201 - 543 0.410 - 604 0.268 - 694 0.192 - 703 0.232 - 707 0.602 Yes 723 0.429 - 730 0.065 - 742 0.403 - 765 0.245 - 768 0.471 - 777 0.276 - 781 0.206 - 852 0.660 Yes 886 0.090 - 913 0.206 - 928 0.213 - 936 0.796 Yes 958 0.333 - 966 0.638 Yes 976 0.209 - 1114 0.160 - 1130 0.127 - 1140 0.838 Yes 1147 0.118 - 1150 0.463 - 1210 0.246 - 1213 0.340 - 1296 0.569 Yes 1348 0.072 - 1404 0.168 - 1419 0.165 - 1440 0.064 - 1511 0.037 -

Explain the output -- Processing locus 609: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCATTGTCCACGTCATCCTTTTTCCCTCTGAAGCCGCCAAATATCAAAAAACACAAAACCTTTACCCGAAGCAAAGTAG CTGCACACTTGCCATTTATAAGGCATGTTTCTTCATCTAAAGCAAAGTATTTTCGAGTTCCTGAGCCAAAAAGATCAAAC AATGTCTAAAGAAGCTGAGATGTCGATCGCGGTGTCGGCTTTGTTCCCTGGTTTTAGATTCTCTCCTACTGATGTTGAAC TTATCTCGTACTATCTTCGTCGTAAAATCGATGGTGATGAGAACTCTGTTGCTGTGATTGCTGAGGTCGAGATTTACAAG TTCGAGCCGTGGGACTTGCCAGAGGAATCGAAACTGAAATCGGAGAACGAGTGGTTTTACTTCTGCGCGAGGGGGAGGAA GTACCCGCACGGGTCACAAAGCCGGCGAGCCACACAGCTAGGATATTGGAAAGCGACCGGTAAAGAGCGGAGTGTTAAAT CCGGGAACCAAGTTGTTGGAACCAAGAGAACGCTTGTATTTCATATCGGTCGGGCTCCTCGTGGCGAGAGAACGGAGTGG ATTATGCATGAATACTGCATCCATGGAGCCCCACAGGATGCATTAGTGGTGTGCCGGTTAAGAAAAAATGCTGATTTTCG GGCTAGTTCGACCCAAAAAATGGAGGATGGTGTTGTGCAAGACGATGGCTACGTTGGCCAAAGAGGTGGTTTGGAGAAGG AGGACAAATCCTACTATGAATCTGAGCATCAGATACCAAATGGTGACATCGCAGAATCATCAAATGTTGTTGAGGATCAG GCCGATACCGATGATGATTGTTACGCCGAGATTCTGAACGATGATATAATAAAGCTCGACGAAGAAGCGTTGAAAGCTAG CCAAGCGTTTCGACCAACTAATCCAACTCATCAAGAAACAATATCAAGCGAGTCATCGAGTAAGAGGTCAAAATGTGGTA TAAAAAAAGAATCAACGGAAACAATGAATTGTTACGCTTTGTTCAGGATCAAGAACGTTGCCGGAACCGACTCCAGCTGG AGATTCCCGAACCCGTTCAAAATCAAGAAAGATGATAGCCAGAGATTGATGAAGAATGTTCTGGCCACTACTGTTTTCTT GGCTATCTTATTTTCTTTCTTTTGGACTGTATTAATAGCTAGGAACTAAAGCTAGTTACGACATACATATTATTTATACA TAAATAAATATAGTATTTGGTCTATGGCAATATGGCATGTTGAGCTAGTCTTAACAATTATTCAAACT ................................................................................ ........................N....................................................... ..............................N.....N........................................... ................................................................................ ................................................................................ ................................................................................ ...i...N..............N..............N.............................N............ ...................i......i.................N................................... ...............N.......................N.......................N................ ..........N..N..........................N....................................... ........................................................................i....... .......................i........................................................ ...............................N................N......N........................ ................................................................................ .......................N.......N....N............................... Pos Score Pred ------------------------ 105 0.420 - 162 0.779 Yes 180 0.795 Yes 232 0.235 - 271 0.328 - 277 0.249 - 564 0.640 Yes 568 0.372 - 583 0.352 - 598 0.244 - 628 0.490 - 660 0.806 Yes 667 0.607 Yes 685 0.325 - 736 0.094 - 760 0.245 - 784 0.431 - 811 0.228 - 814 0.192 - 841 0.142 - 953 0.790 Yes 984 0.656 Yes 1072 0.444 - 1089 0.247 - 1096 0.265 - 1224 0.049 - 1232 0.084 - 1237 0.041 -

Explain the output -- Processing locus 610: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAATGTGTAATATTTAAGAAGATGTGGATCAAATTGAAATATCTAATTATGGGAGTAGAATGTATATTATAAATGTATT GGGTCAATATTTAATAACTAAAACGACATGGATAAGATTATAATATCTAATTTGTCGGAATTTGACCACTCAGTAGTTGA CTATCACAAATCAAGTTTGCTTCTTTGCAAAAGCCAAAGTTTCGAACTTTCTCCAATAGAGATGAGCAAGAACAATAAGA AGAAGAGAAGATGGGATTTGAAGAACGGAGGGATTCTATTAGAGGAGCTAATCGCTTCCTTCGACGGCAAAACCAATCCG ATTCGTTGCTTCTCCTCCGATCAGATCCTTAAAGCTACCGACAATTTCTCCGAAAGCCGAATTATCTCCAGCTGGGGATA CTTCATATGGTACAAAGGCGTAATCGAAGAAAGACAAGTTTCAATCAAGAAATGGTCGAGTCAAAATCTCTCAAGCTTCA CAGAAGCGTATAGAGATATCTCTGTATCCTCTCAGATGAGTGGACACAAGAACGCTTTAAAGCTTATAGGTTGTTGTTTG GAGTTTGATCTTCCAGCTTTAGTCTGTGAATACACAGAACACGGACCGCTTAATAGAGACGGAGGACTCTCAAGTGGAGT GGTTTTACCATGGAAAGTGAGATTGAAGATTGCTAAAGAGATAGCTAGTTCTGTGACTTATCTTCACACGGCGTTTCCAG AGACTATAGTCCATCGGAATATAAATCCGACGAACATTTTCATTGATGAGAATTGGACGGCGAAGCTTTCTGATTTCTGG TTCTGTGTTGCAATACCAGAGGGGGAATTGTATGTAGAAGATGATGTGAAAGGTGTGATTGGTTTTGTTGATCCGGACTA TTACTGGACAATGAAAGTTACTGAGAAAGTGGATATTTATAGCTTTGGTGTTGTGATGTTGGTTTTGTTGTCTGGAAGAG CTGCTGTGTTTAATGGACCAGATGAGGCTCCTATGTCGCTTAATGATCATGTGTCTGAGGTAATGGAGAAAGGTGAGTTT GATGAGATTGTTGATAAAGAGATATGGAATGATTTGGGTGGGGATGATGATTTGGTTTTGAGACGGTCTCAGGTTAAAGC GTTTCTTAGATTAGCGTTGAGATGTGTAAGATATAAGAAGGAAGATCCAGTGAGTGGTATGCTTGAAGTTGCTAAAGAGC TCAAATTGATTGAGAAACTCTCTTGATTAGTCTTCCTTGTCTTGTTAGTTTCTTCATACCTCTTGAAAGAATGATAACTT CTGAGAAGTTTTAGTGATGTCTCAATGTGTTTGAGTTCTTGTGTT ...i..................N..........................N..........N............N...... ...........................N.................................................... .............................................................i.................. ..........i..................................................................... ................................................................................ ......N............................................i............................ ...................................N............................................ ................................................................................ .........N...................................................................... .............................................N.................................. ...............................N........N..N.................................... ..........N............................................i........................ ............N........N..........i.........N.....N.............i................. .....................N....................................N..................... ......................................................................N......... ................N.......N.................... Pos Score Pred ------------------------ 4 0.516 Yes 23 0.251 - 50 0.239 - 61 0.322 - 74 0.335 - 108 0.270 - 222 0.727 Yes 251 0.695 Yes 407 0.304 - 452 0.729 Yes 516 0.456 - 650 0.467 - 766 0.160 - 832 0.153 - 841 0.173 - 844 0.092 - 891 0.477 - 936 0.585 Yes 973 0.105 - 982 0.097 - 993 0.753 Yes 1003 0.062 - 1009 0.134 - 1023 0.631 Yes 1042 0.093 - 1064 0.477 - 1069 0.385 - 1084 0.124 - 1087 0.182 - 1142 0.231 - 1179 0.141 - 1271 0.053 - 1297 0.071 - 1305 0.074 -

Explain the output -- Processing locus 611: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 612: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTTTTTGTAACAAAGTGACCTAATACGTGCTTACTCAAATCCAATTTCAATCATCTAAACCACAATTTGGTGCATACA ATATTTATATATAAAAATTAAATATCTCGTGAGAGTTTTTCTATAAAAGGTACACGTACACTCATGTATCTACTTACTCA CTCCTTTCAGAAACCCAAACCCTCATTCCAAAAAATGGGGTCTCGCTCTCAAAACCTCTCCTTTCTCGTCCTCCTCCTCC TTGGCTTCGTTGCCGTCTCCTATGCTTGCGACTGTACCCCTCCTAAACCATCACCAGCACCTCACAAACCACCAAAACAT CCAGTCAAACCGCCTAAACCTCCTGCCGTCAAACCACCTAAACCACCGGCAGTAAAACCACCTACTCCAAAACCTCCCAC CGTGAAACCCCATCCTAAACCACCAACCGTCAAACCCCATCCTAAACCACCAACCGTCAAACCCCACCCTAAGCCACCCA CCGTCAAACCACCCCACCCTAAACCACCCACCAAACCTCACCCCCACCCAAAACCTCCCATCGTAAAGCCACCAACCAAG CCTCCTCCGTCCACCCCTAAACCTCCCACCAAACCTCCCCCGTCCACACCTAAACCTCCCACGACCAAGCCTCCTCCGTC TACTCCGAAACCACCCCACCACAAGCCTCCGCCTACACCATGCCCACCACCAACACCAACGCCGACTCCACCAGTTGTAA CACCGCCAACACCAACCCCACCCGTCATAACTCCACCAACACCAACCCCACCCGTCGTAACACCGCCAACGCCAACTCCA CCCGTCATTACACCACCAACACCAACTCCACCCGTCATTACACCACCAACACCAACACCACCCGTCGTGACACCTCCAAC ACCAACACCACCCGTAGTAACACCACCAACACCAACTCCTCCTACGCCTATTCCAGAGACTTGCCCAATTGACACGCTGA AGCTAGGCGCTTGTGTGGACGTTCTTGGAGGTTTGATTCACATCGGACTTGGAAAAAGCCACGCCAAGGCAGAATGTTGT CCGGTTTTAGGAGGTTTACTTGACCTAGACGCAGCCGTTTGTCTATGTACCACCATTAAGCTCAAACTTCTCAACATCGA CCTTGTTCTCCCCATTGCTCTTGAGCTTCTTCTCGACTGTGGAAAAACTCCACCATCTGACTTCAAATGTCCCGCTTAAT CGATGTAGAACGTTGAGAGTCTCTGGACGACTCCAAAAGTTTCGTTTCTTCAAGAAATTTGTTAGTTTCATGATCTATTT GGTAATGGGAGGTTAATC ................................................................................ ...............................................................N................ ..................................i............................................. .....................N.......................................................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ .......................................i........................................ ................................................................................ ................................................................................ ................................................................................ .........................................................................i...... ............................................N................................... ..................................................................N............. ..N..................................................................N.......... ....N............. Pos Score Pred ------------------------ 144 0.239 - 195 0.897 Yes 262 0.474 - 680 0.909 Yes 1034 0.534 Yes 1085 0.147 - 1187 0.319 - 1203 0.384 - 1270 0.032 - 1285 0.066 -

Explain the output -- Processing locus 613: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTCCTTCCAATAAGTAGAATTTTATCAGGTGATTGAAGTGAGTATTCGAAGAAAATGTTTAGTCTTTAGAATATTTTCA TTGTTGGTTATGGGATTTTGATGCTGTATTGGTTTTGAACAGGGGATGGGAAGGGTTAAGTTGAAGATTAAAAGACTTGA GAGCACAAGCAACAGGCAAGTTACATACACGAAGAGAAAAAATGGGATTTTGAAGAAAGCCAAAGAGTTATCGATTTTGT GTGATATTGATATTGTCCTTCTTATGTTTTCCCCTACCGGAAGAGCTACTGCTTTCCATGGAGAACACAGTTGCATTGAA GAGGTTATTTCCAAGTTTGCGCAATTAACTCCACAAGAAAGGACAAAAAGGAAACTGGAGAGCCTTGAAGCATTGAAGAA AACTTTTAAGAAACTGGATCATGATGTAAATATACATGACTTTTTAGGAGCAAGGAATCAAACTATTGAGGGTCTAAGTA ACCAAGTAGCCATTTACCAAGCTCAGCTAATGGAGTGTCATAGGAGGTTGAGTTGTTGGACGAACATCGATAGAATAGAA AACACTGAGCACCTCGATTTATTGGAAGAATCATTGAGGAAATCCATTGAAAGAATCCAGATTCACAAGGAACATTACAG AAAGAACCAACTCTTGCCAATAGAATGTGCAACAACACAGTTTCACAGCGGGATACAGTTGCCTATGGCGATGGGAGGTA ATAGTAGTATGCAAGAAGCTCACTCCATGTCTTGGCTTCCTGATAATGATCACCAGCAAACAATCTTACCTGGTGATTCC AGTTTTCTTCCCCATAGAGAGATGGATGGTTCGATTCCCGTTTACTCAAGCTGCTTCTTTGAGTCTACGAAACCAGAAGA TCAGATATGCAGCAACCCGGGACAACAGTTTGAGCAGTTAGAACAACAAGGAAACGGTTGTTTGGGGTTACAACAACTTG GAGAGGAATATTCATATCCTACACCGTTTGGTACTACTTTGGGAATGGAAGAAGATCAAGAGAAAAAGATAAAATCTGAA ATGGAATTGAACAACTTGCAACAACAGCAACAGCAACAACAACAACAACAACAACAAGATCCTTCAATGTATGATCCCAT GGCTAATAATAATGGTGGCTGCTTTCAGATTCCTCATGATCAGTCCATGTTTGTCAATGATCATCATCATCATCATCACC ACCATCATCAAAATTGGGTTCCAGATTCAATGTTTGGTCAGACTTCTTACAACCAGCAACCGAACTAACTCAAGACATGG CTCATGTATGTATGATGACTGGACTTATGTCTGACCAAGGACTAGTTTGAGCCATTGCCTACAATGTCTAAGATAAATAT ATATATG .......................................................i........................ .........N..........N........................N.................................. .........................................N...................................... .......................N.................................N...................... ................................................................................ ....................N..N...........N............................................ .............................i.................................................. ................................................................................ ........................N.......................................N.....i......... ........N.................i..................N.................................. .....................i...i...................................................... ......i......................................................................... ............................................N................................... i.................................................................i...N.......i. ...........i.......................N..........N.........N....................... .............................N..............................................N... ...N...N...N..N...........N....................................N................ ....N.. Pos Score Pred ------------------------ 56 0.696 Yes 90 0.370 - 101 0.280 - 126 0.497 - 202 0.302 - 264 0.471 - 298 0.351 - 421 0.305 - 424 0.252 - 436 0.195 - 510 0.607 Yes 665 0.473 - 705 0.465 - 711 0.605 Yes 729 0.436 - 747 0.736 Yes 766 0.267 - 822 0.767 Yes 826 0.679 Yes 887 0.623 Yes 1005 0.329 - 1041 0.533 Yes 1107 0.692 Yes 1111 0.161 - 1119 0.580 Yes 1132 0.617 Yes 1156 0.169 - 1167 0.334 - 1177 0.367 - 1230 0.409 - 1277 0.454 - 1284 0.056 - 1288 0.108 - 1292 0.118 - 1295 0.149 - 1307 0.096 - 1344 0.051 - 1365 0.035 -

Explain the output -- Processing locus 614: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGTGGCGGCTCATGCGAACTTATAATGAAAACATCAAATATTCGGAAAAATAATACTCCACTGTTAAAAAGAAAACTTA ACAAAGGAATTAAAAATATGAGAGCAAAAGAACACATGCATTTTCTCATGCATGTACTATTATTTATTTTTTTGCAGAGT TGATGTAAAAAATATACACATATATATAGACATACTTTGGTTAGTTATAAACTCGTTCTATTTTCTTCTCCTTTTTCTAT CTTTAGCAATGAACATATCAGTAAACGGACAGTCACAAGTACCTCCTGGCTTTAGGTTTCACCCAACCGAGGAAGAGCTC TTGAAGTATTACCTCCGCAAGAAAATCTCTAACATCAAGATCGATCTCGATGTTATTCCTGACATTGATCTCAACAAGCT CGAGCCTTGGGATATTCAAGAGATGTGTAAGATTGGAACGACGCCGCAAAACGATTGGTACTTTTATAGCCATAAGGACA AGAAGTATCCCACCGGGACTAGAACCAACAGAGCCACCACGGTCGGATTTTGGAAAGCGACGGGACGTGACAAGACCATA TATACCAATGGTGATAGAATCGGGATGCGAAAGACGCTTGTCTTCTACAAAGGTCGAGCCCCTCATGGTCAGAAATCCGA TTGGATCATGCACGAATATAGACTCGACGAGAGTGTATTAATCTCCTCGTGTGGCGATCATGACGTCAACGTAGAAACGT GTGATGTCATAGGAAGTGACGAAGGATGGGTGGTGTGTCGTGTTTTCAAGAAAAATAACCTTTGCAAAAACATGATTAGT AGTAGCCCGGCGAGTTCGGTGAAAACGCCGTCGTTCAATGAGGAGACTATCGAGCAACTTCTCGAAGTTATGGGGCAATC TTGTAAAGGAGAGATAGTTTTAGACCCTTTCTTAAAACTCCCTAACCTCGAATGCCATAACAACACCACCATCACGAGTT ATCAGTGGTTAATCGACGACCAAGTCAACAACTGCCACGTCAGCAAAGTTATGGATCCCAGCTTCATCACTAGCTGGGCC GCTTTGGATCGGCTCGTTGCCTCACAGTTAAATGGGCCCAACTCGTATTCAATACCAGCCGTTAATGAGACTTCACAATC ACCGTATCATGGACTGAACCGGTCCGGTTGTAATACCGGTTTAACACCAGATTACTATATACCGGAGATTGATTTATGGA ACGAGGCAGATTTCGCGAGAACGACATGCCACTTGTTGAACGGTAGTGGATAACGGAAATGGAAGAGAGGAAGTGACTAA GTGAGTGACATACCAAAACATTGTGTAGAAAGATATAATAATCATCATACCATATATATAGTTGTTGAAACT ............N............N...................................................... .................N.................N...........N...N............................ ..N............................................................................. ........N....................................................................... .................................................N.............................. ......................i......................................................... ................................................................................ .......N................N.......................................N............... .......i...................................................N.................... ...N.....................i.............................................i........ .....................................N...............................N.......... ...................................................N............................ ..................................................i............................. ...............................N................................N............... ........i..................................................................N.... .........................N................................N..................... ........................................................................ Pos Score Pred ------------------------ 13 0.380 - 26 0.468 - 98 0.386 - 116 0.257 - 128 0.094 - 132 0.094 - 163 0.108 - 249 0.320 - 370 0.303 - 423 0.639 Yes 568 0.407 - 585 0.296 - 625 0.327 - 648 0.787 Yes 700 0.233 - 724 0.414 - 746 0.717 Yes 792 0.741 Yes 838 0.368 - 870 0.312 - 932 0.306 - 1011 0.584 Yes 1072 0.415 - 1105 0.369 - 1129 0.569 Yes 1196 0.339 - 1226 0.439 - 1259 0.131 -

Explain the output -- Processing locus 615: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTTCAATTACTTCTTCCAAAGGACTTAGCCACACGTCGTGTTTCCAGATATTTCCTGTTTGTTTTTGTTTCTTGTTGCG TACCAGTCTCTTGCAGTCTAAATGGGGGTCTGTTGTAGCAAGGGCACAGGGATAATTGTGGAGCATGGCGCTGATGATGG TAATGAATGTGGAGACGGAGAGGCTGAGGTTAGGGACACAAATGATGGAGCTGTTGTAAGGACGCGTGGATCATCCAAAC ACGTTTCCATGTCAATCAAACAAGGGAAAAAAGGAATAAATCAAGACGCCATGACAGTCTGGGAGAACTTTGGTGGGGAA GAAGATACGATCTTTTGCGGTGTATTTGACGGTCACGGTCCCATGGGTCACAAGATTTCTCGTCACGTATGTGAAAATTT ACCTTCGAGGGTCCATTCAAAAATTAGATCTTCCAAATCTGCCGGCGATGAAAACATAGAGAATAATAGTTCACAGAGTC AGGAGGAACTCTTCCGTGAATTCGAGGACATTCTAGTGACATTCTTCAAGCAAATAGACAGTGAACTTGGTCTCGACTCT CCTTATGATAGTTTTTGCAGCGGTACAACTGCTGTAACTGTCTTTAAACAGGCTGACTGTTTGGTGATCGCCAACTTGGG ACATTCACGAGCCGTTCTTGGCACCCGAAGCAAGAACAGTTTCAAAGCTGTCCAACTCACGGTTGATCTGAAACCCTGTG TCCAACGCGAAGCAGAGAGAATAGTGTCATGCAAGGGAAGGGTGTTTGCTATGGAAGAGGAACCCGATGTGTATAGAGTG TGGATGCCTGATGATGATTGCCCGGGGCTCGCAATGTCAAGGGCTTTTGGTGATTTCTGCCTCAAAGACTATGGACTTGT CTGCATTCCTGACGTCTTCTGCAGAAAAGTCAGCAGAGAAGACGAGTTTGTGGTTCTAGCTACTGATGGGATTTGGGATG TTTTGTCAAACGAAGAAGTGGTGAAAGTTGTAGGTTCCTGCAAGGACCGGTCAGTTGCAGCTGAGATGTTGGTTCAGCGA GCCGCTCGGACTTGGAGAACAAAGTTTCCAGCCTCAAAAGCTGATGACTGTGCGGTGGTGGTGCTTTACCTAAACCACCG GCCTTACCCAAGAGAAGGAAACGTGAGCCGAGCAATATCAACTATTTCCTGGAGATCAAATAAGAGCAATAACGAGTGTT ATGGAGCCGCACCATTGTCACCATTGGGTCTATCCCAAAGGGTTTCATAGTAGGATAATTGTTACTGTTGTTGTTTCTAA CTGTTTAGTAAAATTTGAACCTGTTGAGGTGTCGATATTACTCAACATCAACTTTATTTGTGTGTGAAA ................................................................................ .....................i..........................................N........N..N... ..N...N..................................N..N................................... ........N.........................................N............................. ..........................................N.........................N........... ...............................................N................................ ................................................................................ ....N........................................................................... ................................................................................ ............................i.....................i...............N............. ...N......N..N...................i....................................N......... .................................................................N...........N.. .................................................................i.............. ...........................................N.................................... ................................................................................ i............................................................................... ..................................................................... Pos Score Pred ------------------------ 102 0.821 Yes 145 0.431 - 154 0.194 - 157 0.202 - 163 0.184 - 167 0.426 - 202 0.226 - 205 0.244 - 249 0.411 - 291 0.381 - 363 0.350 - 389 0.228 - 448 0.217 - 565 0.463 - 749 0.570 Yes 771 0.553 Yes 787 0.315 - 804 0.343 - 811 0.334 - 814 0.304 - 834 0.556 Yes 871 0.358 - 946 0.176 - 958 0.246 - 1026 0.634 Yes 1084 0.461 - 1201 0.734 Yes

Explain the output -- Processing locus 616: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACCAACGTAGCTTGATTTAACTAAAACAACTGTCTGTCACCAAACAAACCAAGACGGACGAGTAGATTTGTATGTAATC TCTATACGAATCCGTTTGTGAACTTCATTTGCGTCACCGCCTTGTCACATTCGTGTGGACGCCTCACGACGTGTTTGCAT AATGTTATTGAAACAGAAATATTGCACTCTTCCTCCATTTTTTGATTACTTCCATTTCCTCAAAAGTCAAAGCTCTCTTT CTTCTCTCTCGAGATTTGCTAACTTGTCAATGGCGGATGTTGAGAATCTGAACTCTGTTCCGGCGTCGGAAGACACAGAG TATGGATTTAAACGGCCGGAGATGTACAGCACAAACATAGCCAATTCCATAACTTCCTACGCTCGTCATGTTTTTGTTCT TTACAAAACTCCTGAAGCTTGGCTCTCTCACGTCGAAGAAGAAGGTTTACCTCAACGTTTCGCTACGTTGCTTAAAGATC GCAAATCTGATCTTCTTGTTCAGACGAAGCTCAATGTATGTGAAGGTGGTGGATCTGACGGTGATGTTTTGATTTTTCCC GATATGATCAGATACAAGGGGGTTAAGGATACGGATGTGGAAGGTTTCTTTGAAGATGTTCTTGTGAATGGGAAGCCATG GAGTTCTGGGATACAAGAAGAGATATCTGGAACATTTGTATTTGTGTGTACTCATGCTAGTCGTGACAAGAGATGTGGTG TTTGTGGACCAGTGATCTTGGAGAGGTTTAAGCAAGAGATTGGTTCTCGAGGACTGTCGGACCAGATTACACTGAAACGT TGTTCTCATGTTGGACAGCACAAGTATGCTGGTAATTTGATCATCTTCTGTCCTGATTCAGCTGGAAAGATTACTGGCAA TTGGTATGGCTATGTAACACCTGATGATGTACCTGAATTGCTTGATCAACATATTGCAAAAGGAGAAATCATACAAAGGA TTTGGAGGGGGCAAATGGGTCTACCTGGAGGTGAAGCTGAGAAAGTGCATGAACAGAAAGTTATACCAAATGGTCACGGT GTAGTGAAAGAGGAGAGCAAAGGATTCACAGGAGGATGTTGTCAGGGATCAAACGGTGTTTCGTGTTGCCAAGACGAAAC TCCAAAGCCAGAGCCAATCAAGAAAGAAGTAAAAAAGTGTACCATATGGTTCCAGCCTCTTGACAAAGAAGAGCTTTACA TTGGAGCTGCTGTGGTTGGAGCCATTGCAACTATAGCCATGGCTTTCACCTTTTTCAAGAGGTCAGGTTAAGAACCTAAC CTTACTTACTTACCAATAGAAGTTTTTCCTTGGAGATTGCCATGAGACAATAGAAAAAGAAAACCTCCATTGATTCGACC ACAAAACAGA ........................................................................N....... ................................................................................ .............................i......N........................................... ................................................................................ .................................N...N.........................N................ ...i..............................N....................N...........N.........N.. .....................................................N..................i....... ................................................................................ .......N.................N...................................................... .....i.....N...........N..N..................................................... ..............i.................................N....................i.......... ...................................N............................................ .............................................N.................................. ......................................N......................................... .........................................N...................................... .......... Pos Score Pred ------------------------ 73 0.130 - 162 0.301 - 270 0.759 Yes 277 0.377 - 322 0.353 - 342 0.571 Yes 388 0.339 - 514 0.157 - 518 0.132 - 544 0.058 - 564 0.589 Yes 595 0.141 - 616 0.354 - 628 0.200 - 638 0.311 - 694 0.276 - 713 0.593 Yes 808 0.299 - 826 0.175 - 886 0.548 Yes 892 0.347 - 904 0.255 - 907 0.216 - 975 0.774 Yes 1009 0.210 - 1030 0.687 Yes 1076 0.351 - 1166 0.330 - 1239 0.400 - 1322 0.108 -

Explain the output -- Processing locus 617: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTGAGCTATGGGAGTTGGATTGGTGTGGGTTTTGTCATAAGAATCTTTCTGCGCTCTATGTATTTATATACTTAACAC AGTCGTGTATAATTCGATTAAGCTTTATTTTATTTTTTGATGTTGATTCGAATTTCATATATACACGACGCACACATGCA AATTGATCATCAAAATCTGATAAGAAAAAAACAGAGAGTTAAAACTTACTTACAAGAGGGGTCTGAAGGAAACATAGAAA AGGATTGGGCCGATACTGTTATTAGTATTCAAGATAAAGGTCTAAACCTACAATTTGTAAACAAAATTAGTACTAAAGAT TTTGAGTGTTTCTCTCTACAGTTTTTTGTGTTTATCGTATGTTTCACACAGTACACGCTTCAGTGGGAGTTGTTGGGTGA TCGGTCCGAGGCGACATACAAGATGACCGGAAACTCTTTTAACTCGATGAAAGATTTCCTGAAGCAGACAGGCGGCTATG CGGTAATAGACGGAGGGCTTGCAACGGAGTTCGAGAGACATGGAGCAGATCTCAACGATCCTCTCTGGAGCGCCAAATGC CTTGTCACCTCTCCTCACCTCATTCACACCGTGCATCTCGATTACCTTGAAGCTGGTGCTGATATCATCTCCAGCGCTTC TTATCAGGCCACGATTCAGGGGTTTGAAGCAAAGGGATTTTCTAGAGAAGAGAGCGAATCTTTGCTTAAAAAGAGCGTAG AAATAGCGACTGAAGCTCGGAACTCGTATTATGACAAATGCGGAACTAGCTCTTCCATGGATGATAAGATTCTCAAAAAG CGCCCTATATTAGTTGCAGCATCAGTTGGTAGCTACGGTGCATACTTGGCTGATGGGTCTGAATACAGTGGAATCTATGG TGATTCGATCACGCTGGAAAAGCTGAAGGATTTTCACCGCAGACGACTCCAAGTTCTGGCGGAATCTGGTGCTGATTTAA TAGCATTTGAGACCATTCCAAACAAGATAGAGGCTCAGGCATTTGCTGATCTATTAGAAGAGGGAGATGTGAAGATTCCT GGGTGGTTTTCATTCAACTCAAAGGATGGCGTAAACGTGGTTAGCGGGGATTCCATCAAGGAGTGTATCTCCATAGCTGA AAATTGTGAGAAAGTAGTGGCGGTTGGAATCAACTGTACCCCTCCAAGATTCATAGAGGGGCTAGTTTTGGAGATAGAAA AGGTGACAAGCAAGCCCATACTAGTGTACCCAAACAGCGGAGAAAGCTATGATGCTGATCGGAAGGAGTGGGTGGAAAAC ACCGGAGTTGGGGATGAAGACTTTGTATCATACGTAGAGAAATGGATGGATGCAGGAGTGTCTCTCCTGGGAGGTTGCTG CCGCACAACACCAACCACCATCAGAGCCATCCACAAGAGACTCGTCAACCGCAGATCTCTTTAGCATTCAACTTCACTCT CTTCTATGAATCTCTAGAACCTTGGCAATAGATTTCTACCAAAAACATGAATGTTTGTCTTCCCTTTGCCAATTACAAAT CTA .........N.................................................N.................... .......................................N...................................i.... ................................................................................ ................................................................................ ......................................N......................................... ......................i.......................N..............................N.. .......................................N....................................N... ................................................................................ ................................................................................ ..............................i......N..................i...N................... ....................................................N.......................N... ................................................................................ ..................................................................N............. .........................i...................................................... ................................................................................ ................................................i..i............................ .............i...........................i...i...N.............................. ................................................................................ .....N........................................N...N............................. ... Pos Score Pred ------------------------ 10 0.276 - 60 0.118 - 120 0.397 - 156 0.594 Yes 359 0.295 - 423 0.679 Yes 447 0.219 - 478 0.329 - 520 0.447 - 557 0.194 - 751 0.589 Yes 758 0.222 - 777 0.685 Yes 781 0.110 - 853 0.212 - 877 0.094 - 1027 0.315 - 1066 0.520 Yes 1249 0.510 Yes 1252 0.576 Yes 1294 0.510 Yes 1322 0.780 Yes 1326 0.569 Yes 1330 0.389 - 1446 0.087 - 1487 0.198 - 1491 0.082 -

Explain the output -- Processing locus 618: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTTAGGGAGACTGTGACGGTAAATTTCCTTTCTTACAAAAAAAGAAATATCAAAATTCCTCTTTCTCGGAAAACCCTA GGTGTGTGATTGTGTCTATAAATTCAACCTCAACCGGTGCATACGAAATCAATTAGAGCGATGGCAACAGATGATGATGG TACTCCGAATTGGTCGGAGCTTGTCACAGACATCTTATCATTGGTTTTCAAGCACTTGAGCTTTACAGATTTTGCTCGAG CTAAGACCGTTTGTTCGTCGTGGTACTTTGCTTCCAAATCATCCTCACCTAGAAAAAATCATACTCCGTGGTTGATTCTC TACCAAGACACGCACTGGCTCATGTTTAATTCCGATGAAGAAAAGTTTTACAGAACAGTATACCTAGGTCGCTTTGCCGA GTGTCGTGGCGTGGCGTCTTGTGGGAGTTGGGTCTTGGTCTTTGACAAGGAAATCAATTTCTACATCATTAATCCCTTCA CGCCACAGCTCATTCGTCTCCCACCTCTTGAATATTCAAACACCGGCACAAAGTTCGAGCGACCCGGTAACTATGTATTC CACCTACTCTTCGACGATCGCCACAGAACAAGCCGTGCAATAGTGGGGAATTCGGTTTTGTGGGTAGACGAAAAAACCAA AGACTACTTAGTTGTGTGGTCGTACAAAGAGGCATTTGTTCCGAGTCCCTACATATATTATTGCAGTAAAAGAGGGCAAG AGTGGTTTGAGATTCCGGCAAGCACTTGTGGGAAGCTATTCCGGTGTTTGGATATGGCCTACAAAGACGAAAAGCTTTAC ATACTTACTTCCAATGGTAGCATAAGAATCTTGGACTTTTCTCTTGGAGACTTGCCTAGACAAATCGACAATCATCCATA CTCCCATCGACCGTTTGTTACCGAGTTTCCTAATCATAAGATGGGAATGAGACTAACCACGTCTGGGGATGTTGTGATGA TCCAATGCGTGACAACAGGCACTCACAAAAGAAGCCCGTTCCGAATCTTCAAGTTGAGTAGCATTACAGAGACGAAGGAG TCTTCTCACGAGGTGGTAGGGTGGGAGACAGTGCATTCTTTCGAGGACGAGGAGTCGTTGGTTTGGGATTTGAGCGTCAC TTTACCAACCAAAGGCGTATCCGGAATCAAGAAAGATTCCATATATTACTGTCACACTTCTCTATCATCATCTATTCGGG AAACATGTGCTTATGAGATTCCTACGCACAAGATCACTGCATATGACATTCCTAAGCAGGATATCAAACCTATTCGACAC CGCGGCATCTTGATTGGAGAGGCTCGTTGGTTCGTTCCTTGTTTCGGTGCTGGTGAATAATCCTCTGCACTATAATTAGG ACAAGGCTTTTCACCAATCAACCATGTTTTGTTAGTTTTGGATTCCATTTTGGCTTTTTGTTTTCGATGTGTTAGTAGA ................................................................................ ............................................................i.........N..N..N... ................................................................................ ................................................................................ .....................N............N............................................. ................................................................................ ........................................................................N....... ................................................................................ ................................................................................ .....................................................i.......................... .............N.................................................................. ........................................i.....N.....................N.......N... ....i........................................................................... ................................................................................ ................................................................................ ....N.......N.............................N..................................... ................................................................................ .......................N..........................................N............ Pos Score Pred ------------------------ 141 0.515 Yes 151 0.305 - 154 0.153 - 157 0.180 - 342 0.360 - 355 0.351 - 553 0.310 - 774 0.611 Yes 814 0.232 - 921 0.697 Yes 927 0.365 - 949 0.333 - 957 0.317 - 965 0.663 Yes 1205 0.481 - 1213 0.428 - 1243 0.449 - 1384 0.091 - 1427 0.040 -

Explain the output -- Processing locus 619: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAATATCATTTAGTAGAAAATTAAGAAATAAGCATACCTTTACGAAAGATGACAAATTATATAAACATCATCGTAAAA ATCAAGATATATCACCTATAAATGTTTTTAAACTACATGCAGTACTCGCTCTTCGGTTCGATTCTGACAATTGGAGCAAT GATCGGCGCAGCTATGAGTGGACGAATCGCCGATATGATAGGCAGACGAGCTACTATGGGTTTCTCGGAGATGTTTTGCA TTCTCGGCTGGTTAGCAATCTACCTTTCTAAGGTTGCAATTTGGCTTGACGTTGGGAGATTCTTGGTTGGTTATGGAATG GGCGTTTTTTCGTTTGTGGTTCCTGTATACATAGCTGAAATCACTCCTAAGGGTCTTCGTGGTGGGTTTACAACTGTTCA TCAGTTACTGATATGTTTGGGTGTCTCAGTCACATATCTCTTGGGCTCTTTTATAGGCTGGCGAATTTTAGCTTTGATCG GAATGATACCATGTGTTGTACAAATGATGGGATTATTTGTCATCCCAGAGTCTCCTAGATGGTTGGCAAAAGTTGGCAAA TGGGAAGAATTTGAGATTGCATTGCAACGATTACGTGGTGAATCCGCAGATATTTCATATGAGTCTAATGAGATCAAAGA TTATACGCGAAGACTTACAGATTTATCAGAAGGTAGTATAGTAGATCTGTTCCAGCCACAGTATGCTAAATCTCTAGTTG TGGGAGTTGGTCTGATGGTTTTGCAACAATTTGGAGGGGTTAATGGAATTGCTTTTTACGCTAGCTCTATTTTCGAATCT GCTGGGGTTTCTTCCAAAATTGGAATGATTGCGATGGTGGTCGTACAGATCCCTATGACAACTTTAGGTGTACTGTTGAT GGATAAATCAGGAAGACGACCATTACTTCTAATTTCTGCTACTGGAACATGTATCGGGTGTTTCCTCGTCGGTTTATCAT TTTCATTACAGTTTGTAAAGCAATTGAGTGGAGATGCTTCATATCTAGCACTTACAGGCGTTTTGGTGTACACCGGATCA TTTTCATTAGGGATGGGTGGGATTCCTTGGGTCATTATGTCAGAGGTAAGTAACATTCATAAAAATTCTTTGGTTTTAAA CATACTCTTATACTTAATAATCCGATCGATGTCTTAAGAAATATTAAACTTTATATCATAAACCTACGGTTTAGTATTAA AATTAAATAATAAATTATTTAACTCTCATATGGTTGTATGTGGTATGATTTTGATC ..................................................N............................. .....................N..............N.........................................i. .............N....................N....................N..............N......... ........................................................................N....i.. ................................................................................ ............N................................................................... ..N.......i............N..N...............................N....................N ..........................................................N........N............ ..............................................................N................. ..............i...........................N..................................... ........................i........N....................N.......................N. ................................................N............................... .................................N.............................................. ............N.......................N........................................... ............................N................................................... .............................N.......N......N........... Pos Score Pred ------------------------ 51 0.173 - 102 0.449 - 117 0.258 - 159 0.518 Yes 174 0.232 - 195 0.309 - 216 0.277 - 231 0.166 - 313 0.071 - 318 0.702 Yes 413 0.484 - 483 0.268 - 491 0.696 Yes 504 0.382 - 507 0.459 - 539 0.219 - 560 0.255 - 619 0.248 - 628 0.089 - 703 0.103 - 735 0.523 Yes 763 0.154 - 825 0.589 Yes 834 0.331 - 855 0.187 - 879 0.113 - 929 0.400 - 994 0.391 - 1053 0.355 - 1077 0.199 - 1149 0.348 - 1230 0.069 - 1238 0.078 - 1245 0.036 -

Explain the output -- Processing locus 620: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGTGATTAGATTTTAATCATCAAATAACTTATTCATTAACAAAAAATTAAAATATAAGCACATTTATACTATAGAGACT AGATCCAGTGGCTATAGATAAATGAGGTGGCATATCGGAGGGTCAAAACAGATTGGTGTGGTCCTATTTATTCAAGAAAG GACTGTATCGACAGAAGAAGGGTTTAAAGAAGACAAAGAAATTAAAGGAGAAAAATCGATCACGATGGATATTCGTAGTG ATGATGCAAAAAAACCTATGATGATGTGGTTCTTAGGGATGCTGTTGTTTTCCATGGTGGCCGAGTCAAATGCTCAACTG TCAGAGAATTACTACGCCTCGACATGTCCTAGCGTAGAGCTCATCGTTAAGCAGGCGGTTACTACAAAATTCAAACAAAC TGTCACAACGGCTCCTGCAACGTTGCGGATGTTCTTTCACGACTGCTTCGTCGAGGGATGTGATGCGTCTGTGTTTATAG CATCTGAGAATGAAGACGCAGAGAAAGACGCAGATGACAATAAATCTCTCGCCGGAGACGGATTTGACACCGTGATTAAA GCTAAAACCGCTGTAGAATCTCAATGTCCCGGAGTTGTGTCATGTGCCGATATACTAGCTCTCGCCGCTAGAGACGTCGT CGTGCTCGTTGGAGGGCCAGAGTTTAAGGTGGAGCTAGGGAGACGAGACGGGCTCGTGTCGAAAGCGTCTAGAGTGACCG GCAAGTTACCTGAACCAGGGCTTGACGTGAGAGGTCTAGTCCAGATCTTCGCTAGTAACGGGCTTTCATTGACCGACATG ATCGCTCTTTCAGGCGCACATACAATTGGATCCTCTCACTGCAACCGTTTCGCCAACCGTCTCCACAATTTCTCGACGTT CATGCCAGTGGACCCTACGATGGACCCGGTCTACGCTCAGCAGCTGATACAAGCCTGCTCTGACCCCAACCCAGATGCTG TAGTAGACATTGACCTAACGTCCAGAGATACCTTCGACAATAGCTACTACCAGAACCTCGTGGCTCGGAAAGGACTTTTC ACCTCCGATCAAGCTCTGTTTAACGATCTCTCGTCTCAGGCTACGGTGGTCAGGTTTGCTAACAACGCTGAAGAATTCTA CAGCGCTTTTAGCTCTGCTATGAGGAATCTTGGCCGCGTTGGGGTTAAGGTTGGGAATCAAGGCGAGATTCGAAGGGACT GCTCTGCTTTTAACTAGCTTTATTGAACAAATGTCTATAACCTAAAACATATAAATTATGAAGCTTTTGATTTAAGCTTT TGCTATACTAAAATGTCATAATCCACAATGGAACTT N............................................................................... .....................i.......................................................... ................................................................i............... N..N.............i..i..i..............i..............N...............N.......... .......................i........................................................ ............................i............................N....N................. .........N.......................N.............................................. .......................i.................i...................................... ................................................................................ .............................................................................i.. ................................................................................ ................................................................................ ................................................................................ ...................i............................................................ ..............................N..........................N...................... ............N..............N........ Pos Score Pred ------------------------ 1 0.180 - 102 0.546 Yes 225 0.545 Yes 241 0.113 - 244 0.156 - 258 0.528 Yes 261 0.726 Yes 264 0.572 Yes 279 0.531 Yes 294 0.253 - 310 0.285 - 344 0.549 Yes 429 0.623 Yes 458 0.288 - 463 0.272 - 490 0.295 - 514 0.184 - 584 0.556 Yes 602 0.555 Yes 798 0.502 Yes 882 0.838 Yes 900 0.744 Yes 955 0.541 Yes 1140 0.553 Yes 1231 0.137 - 1258 0.050 - 1293 0.078 - 1308 0.061 -

Explain the output -- Processing locus 621: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGCTTTCAAATCAGTCCGTGACAGTCCATGAACCTTTTAACAGTTGTATAACAGCATTTTGTCAGAAAACAAGGTCACAA CATATACATACACGCACATAGCTTAGAGCCATACGTAATAATATCACACAAATATCAATTATGATGTTCAAAGTCATATT TAATATTTGCTCTTCTCCTCCAATGAGGATGGAGATGGTGCATGCTGACGTGGCGTCTCTCTCCATAACACCTTGCTTCC CGTCTTCTTTGTCTTCGTCCTCACATCATCACTATAACCAACAACAACATTGTATCATGTCGGAAGATCAACACCATTCG ATGGATCAGACCACTTCATCGGACTACTTCTCTTTAAATATCGACAATGCTCAACATCTCCGTAGCTACTACACAAGTCA TAGAGAAGAAGACATGAACCCTAATCTAAGTGATTACAGTAATTGCAACAAGAAAGACACAACAGTCTATAGAAGCTGTG GACACTCGTCAAAAGCTTCGGTGTCTAGAGGACATTGGAGACCAGCTGAAGATACTAAGCTCAAAGAACTAGTCGCCGTC TACGGTCCACAAAACTGGAACCTCATAGCTGAGAAGCTCCAAGGAAGATCCGGGAAAAGCTGTAGGCTTCGATGGTTTAA CCAACTAGACCCAAGGATAAATAGAAGAGCCTTCACTGAGGAAGAAGAAGAGAGGCTAATGCAAGCTCATAGGCTTTATG GTAACAAATGGGCGATGATAGCGAGGCTTTTCCCTGGTAGGACTGATAATTCTGTGAAGAACCATTGGCATGTTATAATG GCTCGCAAGTTTAGGGAACAATCTTCTTCTTACCGTAGGAGGAAGACGATGGTTTCTCTTAAGCCACTCATTAACCCTAA TCCTCACATTTTCAATGATTTTGACCCTACCCGGTTAGCTTTGACCCACCTTGCTAGTAGTGACCATAAGCAGCTTATGT TACCAGTTCCTTGCTTCCCAGGTTATGATCATGAAAATGAGAGTCCATTAATGGTGGATATGTTCGAAACCCAAATGATG GTTGGCGATTACATTGCATGGACACAAGAGGCAACTACATTCGATTTCTTAAACCAAACCGGGAAGAGTGAGATATTTGA AAGAATCAATGAGGAGAAGAAACCACCATTTTTCGATTTTCTTGGGTTGGGGACGGTGTGATCATCATCGATCATGAACA AGACTGGCTTGAGTTAGTTAACAAAAGTTATGAGATGATTTGAGTCCTTGTCGATGTAGTTATGTTGCGTTAATAATTGT ...........................N.................................................... ............................................................i..i................ ......................i.....i.....N......N...................................... ........................................................i....................... i.............................................i................................. .............i.................................................................. ................................................................................ .......................................................................N........ ..........................................................i..................N.. .......i......N......................................................N.......i.. ................................................i............................... ..............N.............................................................N... ........................N.....N.....N.............i........i..............i..i.. .................N.............................................................. ........N................................................................N...... .............................N....N..................N.......N.................. Pos Score Pred ------------------------ 28 0.257 - 141 0.617 Yes 144 0.694 Yes 183 0.526 Yes 189 0.601 Yes 195 0.369 - 202 0.394 - 297 0.691 Yes 321 0.787 Yes 367 0.616 Yes 414 0.627 Yes 632 0.307 - 699 0.506 Yes 718 0.105 - 728 0.526 Yes 735 0.422 - 790 0.102 - 798 0.511 Yes 849 0.718 Yes 895 0.285 - 957 0.382 - 985 0.185 - 991 0.143 - 997 0.296 - 1011 0.835 Yes 1020 0.650 Yes 1035 0.625 Yes 1038 0.845 Yes 1058 0.342 - 1129 0.394 - 1194 0.088 - 1230 0.083 - 1235 0.045 - 1254 0.044 - 1262 0.063 -

Explain the output -- Processing locus 622: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAGTCACATGAGACGAATCAGCCGCAAAACAATTAAAAACGTGGGCTCCCTCAAAAGATTTTGAGATTTATTACAATAT CTATCTCCCGACTTGAAATGACCAAAGTACCCTTTGTTACTTCCCCCCTCTATATATTTATGTCACATGACACTGATACA TATATCCAAAACCAAAAGGGAGAGACTAAGAATGGGGTCACTAGGAGAAGAACCACAAGTAGCAGAAGACTGCATGGGTT TGCTTCAACTCCTAAGCAACGGTACCGTTTTGAGATCCGAGAGCATTGACCTTATTACCCAACAAATCCCATTCAAAAAC AACCAAACCGTTCTCTTCAAAGACTCAATCTATCACAAACCTAACAACCTTCACCTCCGTCTCTACAAACCCATTTCCGC CTCCAACCGTACCGCTCTCCCTGTCGTGGTCTTCTTCCACGGCGGGGGCTTCTGCTTCGGGTCACGTTCTTGGCCTCACT TTCACAACTTCTGTCTAACTCTTGCATCGTCCCTTAACGCCCTTGTCGTCTCACCTGACTACCGGTTAGCACCCGAACAC CGCCTCCCGGCTGCCTTTGAGGATGCTGAGGCGGTGCTTACATGGCTCTGGGACCAAGCTGTTTCCGATGGTGTGAACCA CTGGTTTGAAGATGGAACAGACGTTGACTTTGACAGGGTTTTTGTTGTTGGTGATTCATCCGGTGGGAACATAGCTCATC AACTCGCTGTCCGGTTCGGATCCGGTTCAATCGAGTTGACACCGGTTCGGGTACGCGGTTACGTTCTTATGGGACCGTTT TTTGGCGGGGAAGAGAGGACTAACTCCGAGAATGGCCCCTCTGAAGCTTTGCTCAGCTTGGATTTGCTTGACAAGTTTTG GAGATTGTCACTACCAAACGGTGCAACAAGAGATCACCACATGGCTAACCCTTTTGGACCAACGAGTCCTACACTCGAAT CAATTAGCTTAGAGCCAATGTTGGTGATTGTCGGCGGCTCAGAACTCTTGAGAGATAGGGCCAAGGAATATGCATATAAA TTAAAGAAGATGGGAGGAAAAAGGGTAGATTACATTGAATTCGAAAACAAGGAGCATGGTTTCTACTCCAACTATCCATC TTCCGAGGCTGCGGAACAAGTTCTCCGTATCATTGGAGACTTTATGAACAACTTGTCTTAAGTATTTTAGCATTTGGCTT GTTGAAGGTAATTAAGTGTTTAATTCAATTAGGATTCCATCTCAATAATATTAAGTTTGCTTGGTGATTGGTGAATCATT ........N....................................................................... .................N.........................................N......i............. ...............................i.........................................N...... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ......................N..................N.........................N............ ...........i.................................................................... ....................................................................N........... ...............................i................................................ ........................................i....................................... .................N...................................................N.......... .........i.............................................N........................ ...........................................N.................................... ................................................................................ Pos Score Pred ------------------------ 9 0.292 - 98 0.403 - 140 0.480 - 147 0.620 Yes 192 0.698 Yes 234 0.478 - 583 0.151 - 602 0.223 - 628 0.273 - 652 0.542 Yes 789 0.419 - 832 0.752 Yes 921 0.753 Yes 978 0.369 - 1030 0.220 - 1050 0.633 Yes 1096 0.331 - 1164 0.098 -

Explain the output -- Processing locus 623: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 624: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATAGGATTTTCAAAAAAATTATTATCTTATTAATTTATCGATTTGTGTCACTTTTTACACTGATCCCAATTCGGAACCG GAAGAATTTATTAATTTATAGAGATTATTAATTTATCGAGTATTAATTTATAAAAGTTTAACTGTACTTAAGACTATACA TGGGCTTTCTATGGGCTTCAGCCCAAAATCTGTAATCATCGTTTCTGAGTTGGAGTCGGCCACATATGACATAACTGAGA ATTACTTTTCTCTCTCCTCTGACGGTTCTTTTCTTCCTTCGTCCTCTCGTTTTTTTTGTTTCGTCGTCTCTCTCTCGCGC TCTGAAAAATTCGCAGAATCCGTCATGTCTTCCGGCGTAAATTCAACTGGCTCCGCCGCAGCAGCACCGGAGGTTGACAA GATGTTCTTCTGCTACCAGTGCAATCAAACAGTCACAATCTCAATTTCCTCATCCGCTGATCCTTTTTGTCCAATTTGTA ACCAAGGGTTTCTTGAAGAATACGAAGACCCTAACCCTAATCAATCCCTCAATTTCAACCCTAACTCTTCTGATTCCTTT TTCCCCATGGCCGATCCTTTCTCCACCTTGCTCCCGCTCATATTCGGCTCTTCCGCTGCCGCTCCTTCCGGCATGGACTT CATGAGCTTATTCGGTCCTTCGATGCAACCACAGGCTCGTTCCACTCAGCAGAATCCTCAGTCTGACGCGTTTGATCCGT TTACGTTTCTTCAGAATCATCTCCAGACTTTGCGATCTAGCGGTACGCACTTTGAGTTCGTGATCGAGAATCATCCTTCG GATCCAGGTAATCGTATGCCTGGGAATTTCGGTGATTACTTCTTTGGTCCAGGTCTTGAGCAGTTGATTCAGCAACTAGC TGAGAATGATCCTAATCGTTACGGAACTCCTCCTGCTTCCAAATCCGCCATTGATGCTCTTCCTACTGTTAAGGTAACGA AGGATATGTTGAAATCGGAGATGAACCAATGCGCGGTGTGTATGGATGAGTTTGAGGATGGTAGCGATGTTAAGCAGATG CCTTGTAAGCACGTCTTTCATCAGGACTGTTTGCTCCCTTGGCTGGAGTTGCATAATTCGTGTCCTGTTTGTCGATTCGA GTTGCCTACGGATGATCCTGATTATGAGAACAGGAGTCAAGGAAGTCAGGGGAGTGGTGATGGACAAGGATCGGTTGAGG GTCAGCAGACGCCGAGGTTTAGTATACAACTTCCTTGGCCGTTCAGGAGACAAGATGGCTCTGGTTCAGGCTCGGGAGCA CCTGGTACTGGTGGAGGTAATCTTGAGACCAGGGGTGAAGATTTGGATTGAGGTGTGTATCGTCTTTTCTCTCTTTTTAC CCTTTTGTTTGATTTCCGTGTTGTCTTTATGCCACTGGACTATTTGCGGTTTTTAGTGTGGTTGCGATAA ................................................................................ ...............................................................................N ..........N......................................................N.............. ................................................................................ ........................i....................................................... ................................................................................ ......i.................................................................i....... ................................................................................ ...............i................................................................ .....N...............................................N.......................... .....i..............N.......i............N...N...........N........N..........N.. ................................................................................ ...........N...........N...................................N.................... ......................................................N......................... ................................................................................ ............................N......................................... Pos Score Pred ------------------------ 160 0.260 - 171 0.255 - 226 0.258 - 345 0.833 Yes 402 0.360 - 567 0.850 Yes 633 0.735 Yes 642 0.411 - 663 0.302 - 816 0.651 Yes 886 0.181 - 934 0.092 - 966 0.653 Yes 981 0.202 - 989 0.593 Yes 1002 0.384 - 1006 0.190 - 1018 0.280 - 1027 0.208 - 1038 0.292 - 1132 0.163 - 1144 0.358 - 1180 0.463 - 1255 0.435 - 1389 0.039 -

Explain the output -- Processing locus 625: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCCTTTTTTTAATTACTTGGGATTTCGTGTTCAACAAGTTATTGTTTTGGGCTTTGGTTTAATTTTCCTTTTTTTTTTAG TCTGTCTCATCTCTCATCTGAATTCTGAAACCTGGTTGTTTTAGGATAGAGTCTTCTACTCTATATATAGGACTTAGTGT AAGATTTATTGTTGTTCTTTCGAAGATGTGTTCTTGTTTTTCGATAAATATGGTGTCTAAGAAAGATAAACAGATTCATT CCGACCATAACCCTCAAGATGCTTCCCCGGTAGAGTGGGCGGAAATTCGACATAAGCTACTTGCGTTTCAAGAGAACATT CAATCAGGCTCTGACATTGACGCTAGGTCTGATGATCCACAGATGTGTGGACTTTATGTCTCAGATATCTACGAGTATCT CCGTGAATTAGAGGTGAAGCCAAAGCTAAGACCTTTACATGATTACATTGAAAAGATTCAAGAAGATATAACACCAAGCA AGAGAGGTGTTTTGGTAGATTGGTTAGTGGAAGTTGCTGAAGAGTTCGAACTCGTGTCTGAAACACTCTATCTCACTGTC TCTTACATTGATAGATTCTTGTCATTAAAGATGGTTAACGAGCACTGGCTTCAGCTTGTGGGAGTTTCTGCAATGTTTAT CGCTTCGAAGTATGAAGAGAAACGTCGCCCTAAAGTGGAGGATTTTTGTTACATTACGGCTAATACTTATACGAAACAAG ATGTGTTGAAGATGGAAGAGGATATACTTCTCGCGCTTGAGTTTGAACTAGGAAGGCCTACGACAAATACTTTTCTAAGA CGGTTCATAAGGGTTGCACAAGAAGATTTCAAAGTGCCAAACTTGCAGCTAGAGCCTCTTTGTTGCTATCTGTCAGAACT AAGTATGTTAGATTATAGTTGTGTGAAGTTTGTTCCATCTTTGTTGGCTGCTTCTGCTGTCTTTCTCGCTCGATTCATCA TCCTTCCTAACCAACATCCTTGGAGTCAAATGTTGGAAGAATGCACAAAGTACAAAGCGGCTGATCTACAAGTGTGTGTG GAGATCATGCTTGACTTGTACCTGAGCAGAAGTGAAGGTGCTTCAAAAGCTGTTAGAGAGAAATACAAGCAACATAAGTT CCAATACGTTGCGGCAATTCCTGTCTACCAAGAGCTTCCGGTTACCTTTTGGGAGGATGTTGTTACCATTTGATCAGAAC CAATCTTTCATTTCCCAATTGTTACTGAGCTCGATTACTAAGGGACACTGATCAACAGTTGAAGCTAGAGGAGTCATATG TTATATGTATCG ................................................................................ ................................................................................ .........................i.......................i.............................. ..................N............................................................. ...............................N..........i............N........................ ......................................N......................................... ................................................................................ ..............................i.........................................N....... ...........N.................................................................... N..........N.................................................................... ................................................................................ ....i........................................................................... .............................i..........i....................................... ......N......................................................................... ........................................................N....................... .............................................................................N.. ....N....... Pos Score Pred ------------------------ 186 0.566 Yes 210 0.657 Yes 259 0.224 - 352 0.103 - 363 0.540 Yes 376 0.405 - 439 0.315 - 591 0.781 Yes 633 0.374 - 652 0.224 - 721 0.328 - 732 0.408 - 885 0.662 Yes 990 0.639 Yes 1001 0.628 Yes 1047 0.281 - 1177 0.221 - 1278 0.039 - 1285 0.055 -

Explain the output -- Processing locus 626: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAATAAGGTGATTAGTGTCAGTTGTTTTTTAACGACATCATTAGAAAATAGAAACCAATTTTGAAACACAACGACAACA AGTCACAAAATTATTATATAAAAAATAAATTACAGCGAGAAACTAAAACGGCCGGTTCAGATAGAAATCATTAAACCGAG TAATTGGTTAACTGGTCTAATCGTATTTGTTTTTATTTCTCCAATGAATGAGCTGACGGAGCTTCACATCGCCGAGAGCT TCGTCACGAGATCCATGGCGGCCATTTCTCCTCTCTTCCTTCTCTTCCTCATTCCCATTATCCCACTATCGCTTCTCGCT ATCTTAGCCCTAATTGTACGTCCTCGTCCCATTAAGATCCCTATCAAGTCCCGTCACGCCTTCATCACCGGTGGATCTAG CGGCATCGGTCTTGCTCTCGCCCACCGCGCCGCTTCCGAGGGCGCTCGTGTCTCAATCCTCGCCCGATCCGGATCAAAGC TCGAGGAGGCTAAGAAATCGATCCAGCTCGCCACTGGTGTCGAAGTCGCCACATTCTCTGCAGACGTTCGCGATTACGAT GCAGTCTCGAAGGCGATAGATGAATCGGGACCGATCGATGTGTTGATTGTTAACCAAGGTGTGTTTACGGCGAAGGAGCT GGTGAAACATAGTCCTGAAGATGTCAAATTCACGATTGACGTAAATCTTGTTGGGAGCTTCAATGTGATTAAAGCCGCTT TGCCTGCCATGAAAGCAAGGAAAGATCGTGGACCTGCTTCCATCAGTCTCGTCTCCTCTCAAGCTGGTCAGGTTGGTGTC TATGGTTATGCTGCATATTCAGCAAGCAAGTTTGGTCTTCAGGGTTTAGCGCAAGCATTGCAACAGGAAGTTATATCTGA TGATATTCATGTCACTCTTATTTTTCCTCCTGACACTAATACACCCGGCTTTGAAGAAGAACAGAAGAGCAGACCTGAAG TCACTGCCATAATAGCTGCATCCTCTGGTTCAATGGAAACAGAAGAAGTAGCAAAAAAGGCTATGGATGGCATAAAAGCA GGAAACTTCACCGTCTCATGCAACTTTGAGGGATTCTTACTGTCTCTCGCGACAACCGGCATGTCCCCTCAAAGATCATT TTGGCTTGCTTTTCTTGAAGTTATAACCGCAGGGCCTATAAGATTAATCGCTCTCTTCTTTCAATGGGATTGGTATAAAG CCATTGAAAAATGGAGCAAAACCAAAACCAAATGACAGCCCGACAATGTATCTTGAAGTGCTTTTGGCAGAAGCTTAAAC ATTGATGGGTATTCTCAAACCTTCTTCTCAGTCCGGTACCACGCCAGCTAGTCG ................................................................................ ................................................................................ ...........................................N...N................................ ..............i................................................................. ................................................................................ ................................................................................ ..............................................................................N. ...................N.................N.......................................... ....................N.........................................N................. ........i....................................................................... ........N....................................................................... ................................i.............................N...N............. .................N..........................................N................... ...............................................................N................ ..........i....................N.............N.................................. ....N................................................. Pos Score Pred ------------------------ 204 0.465 - 208 0.099 - 255 0.699 Yes 559 0.257 - 580 0.315 - 598 0.344 - 661 0.296 - 703 0.188 - 729 0.633 Yes 802 0.104 - 808 0.068 - 880 0.286 - 889 0.109 - 993 0.631 Yes 1023 0.473 - 1027 0.494 - 1058 0.336 - 1101 0.271 - 1184 0.321 - 1211 0.514 Yes 1232 0.383 - 1246 0.420 - 1285 0.175 -

Explain the output -- Processing locus 627: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATATTAGTTTCTTTCTTCTTCCCTTCATTCCTTCAACTTTTATACCATTTTCCTGTTATCTATTGATTTGAAAGTTTTA ATTTTTAAGAGGCGGTGATGACAAAAAACATTTCTCGATGCTGAAACTTACTCGAGAGACCAAATCACTCTTGTAATCTT TCGTGATGCTTGTTTTTATGCGTGTCTGAGCCTAAACCAAAAAATCTTTCATTTATGCTTTGATTATCATAGATCCCAAT TTATGCGTTTCTTTTATTGCTTTTCAATTTGTAATGCGAGTTTTGCTTTATTTTGTCCAGCCATGGAAGAAGCAGATCAG TTATGTTTAGCTGCTAAATCCGGCGACTTGAAAAAGGTTCAAACTTTAATTTACTCCGGCGCCGACGTTACTCACTTTGA TAACGACGGTTTAACGCCGTTAATGCACGCCGCTAAAATCGGAAACGCAGAGATAGTAACGGCGTTACTCGAATCCGGAG CTCCATGGAACGCATTATCTCCATCAAATCTCTCAGCAGGAGATTTCGCCATGGAAGCAGGACATCAAGAAACTTTCGAT CTTCTTCTCAAAACCGGGATCCAATCAGAGCTAATCTTAGGAACAATCGCAAGAAACCAAACAAAGAACGAATATTCTAA TCAAGAATATCTTCAAGACAGAGTTAGTTTCAGTGAAGACAAGTTAATGGATAGTGAAAGCAAAGGTGTGATGATGGCTT GGGAAAAACCATTAATGGAAGCTCACGCTAAAGCTATTTGTCTCAACGGTGGTCACATTCTTAACGTTGGTTTCGGTATG GGACTTGTCGATACAGCGATTCAACGATACAATCCGGTTAAACACACGATCATTGAAGCTCATCCTGAAGTTTATAAGCG TATGATTGAATCTGGTTGGGGAGAGAAAGAGAATGTGAAGATTGTTTTTGGAAGATGGCAAGATGTTCTTGATAAGCTTG ATGATAATTCTTTTGATGGGATCTTTTTTGATACTTATGGTGAGTATTATGAAGATTTAAGAGAGTTTCATCAGCATCTT CCGAGGCTGTTGAAGCCTGATGGTGTTTATTCGTATTTCAATGGGTTTTGTGGAAGCAACGCGTTTTTTCATGTTGTGTA TTGCAATTTGGTGACTTTGGAGATTGAGAATTTAGGGTTTAGCACTCAGTTGATTCCATTGCCGGTTAAGGATTGTCTTG GGGATGAAGTTTGGGAAGGTGTCAAGCAAAAGTATTGGCAGCTTGATACTTATTATCTCCCTGTTTGTCAGTTTAGTGAC TGAAGAAGTTTTATGATACCAGCAATTTTGCGATATTGTAATTTTTCTTACTCATAAAAAGTAGATTTTTTATCCATTTG AAAATAGTCATAGTCATCCAAT ................................................................................ .................i...................N.......................................... .....N...........N....................................N......................... ..i..............................N............................i................. ..N............................................................................. ......................i......................................................... ....N.............................................i............................. ................................................................................ ..............................................i.......................N..i...... ..............i..............................................................N.. ................................................................................ N..............N....................N...........N............................... ...................N....................N.............................N......... ................................................................................ ...N............................................................................ ............N................................................................... ...................... Pos Score Pred ------------------------ 98 0.503 Yes 118 0.304 - 166 0.257 - 178 0.156 - 215 0.133 - 243 0.629 Yes 274 0.411 - 303 0.738 Yes 323 0.274 - 423 0.715 Yes 485 0.236 - 531 0.578 Yes 687 0.615 Yes 711 0.441 - 714 0.832 Yes 735 0.781 Yes 798 0.399 - 882 0.456 - 913 0.095 - 935 0.191 - 943 0.161 - 961 0.093 - 976 0.086 - 997 0.308 - 1009 0.184 - 1060 0.188 - 1081 0.142 - 1111 0.068 - 1204 0.415 - 1293 0.061 -

Explain the output -- Processing locus 628: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCCATTCTCTTCTCTAACACCCAACCCACAATTAAATTTTAGCAAAAAAATATATAAATAATATAAAAATCATTACAT ATTTTATATACATATATATAGATGACCTGCATTGAGAAAACCCTTTCAAGATTTGGTGGATCATTCAAGAAACACACATA TTCAAACTTCTCCAAAGAAAAGAAAATAGCAAAAACTAGAATGAAGAAGACAATGGAGGATCCAAGCAAGATCATGAGAA GGTCAATTCACACTTTCCTCCAAAACTACCACCGTGTCACCACCGCAGCCGCCGTGGCCCTCCCTTTCTCCGCCGGTCTC CTCCTCTCTCAGCCTTTCTTCTCTTCTTCCTCTTCCACCCTTCACATGAGGCTTAACATGCTCTTCCGCGGAGCGGGTTT CTCTTCTTCTCATGACTTCTTCAACATCTTAAGCCTTAAACTCTCACAAACCCTATCTTCCTCTTTATTCACTCTTCCTT TCTCCCTCACATTCCTCCTTTTATCCAAAGCTTACGTCATCAAACTTCTATCAAACAATCACTCTGCAGATTCCTCCTCT GTTTTCTACCTCCGGCTTCTCAAAACTTACGTTTGCAACTTCTTCTTCCTTCTCTCTGCAAACGCCTCTGCTTTTGCTCT GTTTTTCTTAGCATACAATACCCTTGAAGCCTTTGGATTCTCTTCAAGAAACTTCTACACTTTCCTCTCCCTATCCTCAG CAATCATCTACTCAATTATCATCGCAAACGCCTTTGTCATCTCTAATCTAGCCTTGGTTTCATCACCTTCTTCTTCCTCG GGAGGATACACAAACATCCTCAAGGCGTGTCTTCTGATTCGCGGAAGAAATTCAACAGCAATGGCTCTTGCTCTGCCTAC CAATCTCGGCTTAGCAGGAGTCGAAGCCTTGTTTCAGTACCGAGTCATGAGATCTTATTACAATGGAGACAGAGATATCA TATCCATAGCTCTTGAAGGAACCTTCATAGCTTACTTATACGCTCTCTTCTTGGTTCTTGACACCATAGTCAACTTCCTC TTTTACCAGAGCTGCATCAAAAACGAGGAAGATCAAAATATCGGTAGAGAAGACGAGTATTCAATCAAGATCCAAATCAG CGAAACAGAGAACACAAAGATACACATCAAAGGATCAAAGAACTTGCAAGAAATCTTGTGACTCTCTTGTGTTGGTTGAT GTAATTATTACTTATACAAAAAAAGTGAAAAACTTTATGAGATTATTGATTCTTTAAGGATAATCTCAAGTAGCTTTCAC ................................................................................ .....................N.......................................................... ........................................i...........i....................N...... ................................................................................ .............................................N...........N...................... ...........N.................................................................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ............................................................i................... ..............................................N...............N................. ................................................................................ ................................................................................ ..............................................................................N. ....................................N........................................... Pos Score Pred ------------------------ 102 0.466 - 201 0.813 Yes 213 0.801 Yes 234 0.312 - 366 0.261 - 378 0.482 - 412 0.377 - 861 0.634 Yes 927 0.334 - 943 0.244 - 1199 0.037 - 1237 0.048 -

Explain the output -- Processing locus 629: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTAAACAGGAAAATACCTGAAAAAGTTAAGGCAAAGGTGGCTACTAAATTATTTATATATCTACGTTCCCTTAGCTCT CTGAACTTGAGTCTAAGATAAAGAAACTCTCGACGACATCTTGAGAATCGAACGAGAATGATCCAAAATCTGCAAAAATT ACCCCAAGACCTCCAATCCTCGGCAATGAAGATGCCTCCTCCCTTTTCTGCGTCGAAGACTCAATACTTGTTCCACGACG AGTCATCGGAAAACTCTAAAAAGAGTTTGGTCTCCACAACTCTTTCTTTGTCGTCTTGTGAAAACCCTAATAAGAGGAAG ATGAACTCTGACGAAGTTCTCAATATTTCTTGCATACCAAGAGACTACAAATTAACACAAGTAGAAAGAAAGATTGCACG AATGAGGAATCTCTCGTATCAAGAAAAGGCAGAGGACGAATGGTACGGTGTCTCAACAGAACTAACACTCTTCAAGGATC CGTGGATCATCAAAAAGGTTTTTCACTTTGCTTCAGTCTTGGATATGGCTCCCAACTCAGTCTCAAATACTCATTGCTTG TTGGACACGGAGTCACCAGAAAACGCTGAAGAGAGTTTGGTCTCCCTGGATCTGTGTTTCTATGACAAAACATGGCCACA TGATCCCAATGTTGCTTACAATAAACCAACGTCCGAAGAAGCAATAAACCTGGCGTGGATGAGGACTATGTCGAAGAGAG CAAGAAAAGAAGAGGAGAAGTACTACGTCTCGACAGAACTAACTCTCCTCACCGTCGCTGACCCATGGACCTTGAAGATG GCAATGACCAAAAGCAGTATTGGCAACCTATACCGTCTTGTTCTAAAGGCGAGCTTTGTAGACATCCACATTCTGAGATA TCTCCCTTTGGATGATCAAATGATGGTCAAAGAAGACTCTGGATTAGCAGTCGAGGTGTATGATCACGACACTGACTCTG TGCACAATCTGGCTCTTAAGAAATGGGCCAAATCTTCAAGCTTTGTTCTCGTCAGCGGATGGCGTAAGTGCTTTGTCGAC AGAAGAGGTTTGCAGGTTGGCGATGTGATTGGAATGTATTGGGATCGTTCTGAATCTAAGCTTCATTTTTGTGTGCTTTC TCGTTCCGAAACAATGGATTCAGCTCCTCTTCCTCCAAGTCCATAGGACCTCACTAGCTATGGAGGCTCTGCAGAGAGAT TCTTATTTTCTTTATGTAATCGTCAAACAAGTGATTGGTGTTGTTTCTGTTTTTTTGTTGTTGTC ................................................................................ .........................................................N...................... .........................N.....i................................................ ................................................................................ N............................................................................... ............................................N................................... .............................................................N.........i.......N ........N.................................................N........N............ ................................................................i............i.. ...i............................................................................ ...........N.......i..i....................................N.................... ......................i...................................N..................... ......................N..........i.............................................. .............i.............................................N.................... .............N................................................... Pos Score Pred ------------------------ 138 0.334 - 186 0.465 - 192 0.547 Yes 321 0.471 - 402 0.259 - 440 0.430 - 525 0.427 - 622 0.300 - 632 0.849 Yes 640 0.216 - 649 0.194 - 699 0.287 - 708 0.449 - 785 0.650 Yes 798 0.803 Yes 804 0.550 Yes 892 0.091 - 900 0.651 Yes 903 0.588 Yes 940 0.467 - 983 0.607 Yes 1019 0.186 - 1063 0.310 - 1074 0.659 Yes 1134 0.658 Yes 1180 0.412 - 1214 0.049 -

Explain the output -- Processing locus 630: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTCTTTGTTAACAGTTCCGTGAACTCAATTCATAGAGCTAGTCTGATTATATCTTAGGTTTTAGTGAATTAGTCTGAA TATTAATCAGCTGTGAATTGAATGAAACAGATGCCTCGAAGACCATCATCAGGAAGAAGAGTGTTAAAGCATCAGCCTTT AACATTTTCACCGTTTATGCGGTTACTTTCCTTTGCTTCAATGGCTAGGCGTGATTTGTCTCATCCTGAAGATTGTCAAT GTTCTGATGAAGATATATCTTTTGATGAAAAGCAAAAGATCCCTAATTTACCAAGAAAAGGGAAAGAAGAACAAGTCTCG GACTCGTCTGATGAAGAAGATTCTCAGGAAGATGTCCAAGCAGACTTTGAGTTCTTTGATCCTAAACCTACAGACTTTCA TGGTGTTAAGATCCTGTTACAAAACTATCTCGATGACAAGGAGTGGGATTTGAGCAGTTTTGTTGATTGCATATTGGAGC AAACAACTGTAGGAACTGTTGTGAAAGTAGCTGATGATGAGGATGAGTCAGTATTTGCTCTTGTCACTGCTCTTAACATG GCAAGAGATAAGGACAACAAGTGTTTTAGAGAGTTAAAAGAGTTTCTTCGTAAAGTTTGTTCTGAAAAGAATATAGCGAA TAATCTAGAAATGCTCTTAGAGAAAAAGGCACAAGATGTTGGTTTATTGGTATCTCAAAGAGTGATGAATCTTCCTCCAC AACTTCTTCCTCCACTTTATGATGGATTGTTCGATGAAGTCTCATGGGCAATCGAAGATGAGCCTACAGAAAAGCTTCGA AGATCATTCCGTTTTAAGTCATATCTTCTAGTTACCAAGATTTACAAGCTGAAGAATCCTAAGCAGAGAAAACCTCGACA TGGTGAAGAAGACATTGAAGACACAGTATTTCTTAAGCCTGAAGATGAACTCTTTCTCGAGCTTAGCTCTTGGTCCTTCA CATTCCCTATGCGCTCACAGCTCGTTACTTCCCAAGAAATGAAGAATTATCAGCTGATGGGTTTAGTGATGGCCGTGGAA GCAAACAAGATTCCTAAATTCAGACAGATGTTGAACTCTCTCATTGATTAACAATGATGAACATCTTCTTCTTAAAGATT CAATTAACGGTTTAGTTGTAATCGATTCCCGCCAGTTTTGTTTGATTCGGTTAAGGATTATTGAGGCTTG ................................................................................ .....................N........N................................................. ................N.......................i.....................................N. ......N.................N....................................................... ..........N....................N...............................................N ................................N............................................... .................................N..N.....N..................................N.. ................................................................................ ..........N........................i............................N............... ..................N..N...........N.........N.............N...................... ...............................................................................N ............................................N................................... ........N.............................N.................N...........i........... ...........................N.........................N..N....................... ...................................................................... Pos Score Pred ------------------------ 102 0.107 - 111 0.148 - 177 0.334 - 201 0.585 Yes 239 0.425 - 247 0.319 - 265 0.334 - 331 0.211 - 352 0.125 - 400 0.202 - 433 0.086 - 514 0.158 - 517 0.125 - 523 0.147 - 558 0.387 - 651 0.419 - 676 0.538 Yes 705 0.478 - 739 0.189 - 742 0.168 - 754 0.351 - 764 0.428 - 778 0.246 - 880 0.491 - 925 0.175 - 969 0.486 - 999 0.392 - 1017 0.360 - 1029 0.560 Yes 1068 0.103 - 1094 0.132 - 1097 0.139 -

Explain the output -- Processing locus 631: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTTGATGATGATTAATTTAGTCATTTTGGTTAAGAAAGTGGTTATTTTTAAAAATGGGCCAAAAAAGGATATCATAGT AGAATTGTGGTTTGGGCTTAAAAGCCCATTACCCCTCCCCTAATTAGTTTTCAACTTTTCATGGAGTCTTCGGTTTTCGA CTTTGGATCGGAGTCGGGGAACATGAATTTCGACGAGACCGTGCCACTGAGCCAACGATTGGAGTGGTCAGACGTGGTCC CATTGACTCAGGACGATGGTCCGAATCCAGTGGTGCCAATTGCCTACAAGGAAGAGTTCCGCGAGACTATGGATTACTTC CGTGCGATTTACTTTTCCGACGAGCGATCTCCTCGCGCACTACGACTCACGGAAGAAACCCTCCTCTTAAACTCCGGCAA CTACACAGTGTGGCATTTCAGGCGCCTAGTACTCGAGGCCCTTAATCACGACTTGTTTGAAGAACTCGAGTTCATCGAAC GCATTGCTGAGGATAACTCTAAGAACTACCAACTGTGGCATCATCGGCGATGGGTTGCAGAGAAACTGGGTCCTGATGTT GCAGGGAGAGAACTTGAATTTACCCGTAGAGTACTTTCACTTGATGCCAAACATTATCATGCTTGGTCACATAGGCAGTG GACACTACGGGCATTAGGAGGATGGGAAGATGAGCTCGATTACTGTCACGAGCTCCTTGAAGCTGACGTCTTTAACAATT CCGCCTGGAATCAGAGGTATTATGTCATCACCCAATCTCCTTTGTTGGGAGGCCTAGAAGCCATGAGAGAATCTGAAGTA AGCTACACAATCAAAGCCATTTTAACCAATCCTGCAAACGAGAGCTCATGGCGATACCTAAAAGCTCTTTACAAAGACGA CAAAGAATCCTGGATTAGTGATCCAAGTGTTTCCTCAGTCTGTTTGAATGTTCTATCCCGCACAGATTGCTTCCATGGAT TCGCTCTGAGCACCCTTTTGGATCTTCTATGTGATGGACTGAGACCAACCAACGAGCATAAAGACTCAGTGAGAGCTCTA GCTAATGAAGAACCAGAGACTAACTTGGCCAATTTGGTGTGTACTATTCTTGGTCGTGTAGATCCTGTAAGAGCTAACTA TTGGGCATGGAGGAAGAGCAAGATTACAGTGGCAGCAATTTGAATATGTGACGCCCCAAAATCACACTTGAAAAAGACTT GATTATTAGTTTTTACGTATGTACTGCTTATTTATGAATCACATGTTCATGTTAACATGTAT ......N..N.............................................N........................ ............................................................i................... ......................i......................................................... ...............N....................................................i........... ................................................................................ ................................................................................ .................................................N.........................N.... ...........................................N..............i..................... .....................N.......N.................................................. .....................N........................................i................. ...............................................N................................ ...............................................i..........................N..... ............................N....N.............................................. ....N........................................................................... ......N......................................N.................................. ..................N..............N........N.....N.......N..... Pos Score Pred ------------------------ 7 0.340 - 10 0.454 - 56 0.350 - 141 0.638 Yes 183 0.851 Yes 256 0.240 - 309 0.633 Yes 530 0.498 - 556 0.309 - 604 0.411 - 619 0.512 Yes 662 0.180 - 670 0.440 - 742 0.224 - 783 0.775 Yes 848 0.208 - 928 0.700 Yes 955 0.376 - 989 0.208 - 994 0.157 - 1045 0.236 - 1127 0.372 - 1166 0.103 - 1219 0.039 - 1234 0.056 - 1243 0.063 - 1249 0.041 - 1257 0.065 -

Explain the output -- Processing locus 632: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTTGAATAGTTTTTTACGATGATAAGAGTAAGCGGGAAGCCATAATAGACCACTATCCCAAAACTTTAATCTGCTTCCA ACTCATTATGGGACCAACTAATACAAAATGATATGTATTCCATGTTCCAACCACTCCAATACGAATTACGAAGCAAGCGT GTCTCAGTGTCTGAGTGCGACTGCCTTCTCTCTATATATAAGAGATATACATAGTGAATTAAACATCTTAATTATACTGA TCTTTATCATGTTGACTAGATTTAAGAAACAAAATAATAAGATGGTTAGGGCAAATATAGTGAGCATGGTTCTGTTGATG CATGCCATCGTTGGGTTTCCTTTTCATGCGAGGGGATTAAGTATGACTTACTACATGATGAGCTGTCCTTTCGCTGAACA GATTGTGAAGAACAGTGTTAACAATGCTCTTCAAGCCGATCCCACTTTAGCCGCTGGTCTTATCCGTATGTTGTTCCACG ACTGTTTCATCGAGGGATGTGATGCGTCAATTCTGCTAGATTCAACGAAAGACAACACTGCGGAAAAGGATTCGCCTGCG AATCTGAGTCTACGTGGCTACGAGATCATCGATGATGCAAAGGAGAAAATCGAGAATAGATGTCCAGGAGTTGTATCATG CGCAGATATAGTTGCCATGGCTGCTAGAGATGCTGTCTTTTGGGCTGGTGGTCCATATTATGACATACCGAAAGGAAGGT TTGATGGTAAAAGATCAAAGATAGAAGATACAAGAAATCTACCTTCACCTTTTCTTAATGCCTCTCAACTCATTCAAACT TTTGGCCAACGTGGTTTCACTCCACAAGATGTTGTTGCTCTCTCTGGAGCACATACCCTAGGAGTTGCACGATGCTCCTC CTTCAAGGCTAGACTTACCGTCCCAGACTCTTCATTAGACTCGACTTTTGCAAACACTCTCTCTAAAACATGCAGCGCGG GTGACAATGCAGAGCAACCCTTTGATGCTACGCGCAACGATTTCGACAATGCTTACTTCAATGCGCTTCAGATGAAATCA GGAGTCCTCTTTTCAGACCAAACTTTATTCAACACACCAAGGACCAGGAATCTTGTTAACGGCTATGCACTTAACCAAGC CAAGTTCTTCTTTGATTTCCAACAGGCCATGCGCAAGATGAGCAATCTTGATGTTAAACTTGGCTCTCAAGGTGAAGTCC GTCAAAATTGTCGGAGTATTAATTAGGCTAGACCACATCTACTTACGATTTTTGTATTTATAATAAGTTCTCTACTCTTG TTATATGAAGAATCATGAATCTTCTAGGCTACTATGTAAAAATTG ...................N............................................................ .......N...................i....N........N...................................... ................................................................................ ........N................................i.......................N...........N.. .......................i...........................................i............ ................N....N.......................................................... ...............................N..N........................N.................N.. ................i............N.............................N.................... ...N.....................................................N...................... ............................N..........................................N........ .....................................................................i.......... ......N.................N.......................N...........N..........N........ ................................................................i............... ............................N........N............N............................. ................................................................................ ....N.........N..................N........... Pos Score Pred ------------------------ 20 0.460 - 88 0.313 - 108 0.681 Yes 113 0.340 - 122 0.130 - 249 0.367 - 282 0.627 Yes 306 0.165 - 318 0.264 - 322 0.237 - 346 0.146 - 363 0.711 Yes 375 0.318 - 378 0.589 Yes 424 0.511 Yes 468 0.536 Yes 497 0.382 - 502 0.183 - 592 0.142 - 595 0.255 - 620 0.417 - 638 0.294 - 657 0.792 Yes 670 0.379 - 700 0.140 - 724 0.168 - 778 0.293 - 829 0.141 - 872 0.451 - 950 0.591 Yes 967 0.307 - 985 0.250 - 1009 0.331 - 1021 0.341 - 1032 0.281 - 1105 0.564 Yes 1149 0.281 - 1158 0.425 - 1171 0.267 - 1285 0.070 - 1295 0.041 - 1314 0.049 -

Explain the output -- Processing locus 633: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACGAGAATTTGTGTGTTTGAGTGGTCATTGAATCGAACTCAAATTTAGCATTTTTAGGATTTTGAGTCAATTCTAGTTTA GGATTCGTCTCTAATGTTTAATTTAGTGAAGGCTATGTCATTTTCAGGAATCTGATTGTTTTCCGTTTAAAGATGAGAAC TTTGATGAATTCTGCTGCTACAATGAGAGCTGTGAAGAAGGAGTTTATTGTAATAGACTTAGATGCTGATAAAGATGAAG ATGTTCGTTGTGGTAAAGTGAAAGAAGAGTATATGATGGATGCTGGCAAAACTAATCCTTCCAACATCCAACAGGTTGTT TCTGCTGTTGCTTCTGATGATGATGGTGATGATGATGATGACTGGGGTACCAAAATGGATTATCAGTATATAAAGTTGTT GGATAGTTTCAGAGAAGATGGAAGTTTAAATCTGAGTGACAATCCACTAAGGTCTATTAGGTATGAAGTAGATAATGGTG GATATGACAAGCGGGAGTTTAAAGCAGTGTTTACAAATAAGCGGAGTAGAGAAGATCGAAACACTATTCGTGTAACCAAG AAGAATGTTGAGCCAAAGCCTCCTCATGTTCAAGCAAGTACAGAGAAGGTAACAAAAGAAGCCGGTTTCAAATTAAGGCG AAGAGAGAGTCTTGTGAGTCAGAAGAGTGTTGAAGCAATGCTTATTACAAGTCATGTGAGAAGAAACTCCCAACACAATA ATGGCGACGCTAGCGAGAAGGAGAATTTTGAGGATGACATTGTATTGGATGAGGTTTACAGATCTTATCTCAAACGGTTA GTGGAAAATTCGAAAAGTTCAAGAACTAATCCTGAAAAAGAGATAAAAGTAAAGTGTGAGGAGGATCATACAGTGTCTTT GTCTGATTCTGATTCTGATATTATTGTGGTTGGAGATTGTCCGTTTCTGGAAGAAGAGGATTCTCCATTTGTGCCATCAA AAAGTTATAAAGTGATTGATTTGGATGAAGAGAGCAGTGATCAACGCAATTCTTGGTTTAGGAGGGAGATAATGAATGTT CTGAAGCAGCCATACACTGTAACAGAGCTCAAGGAACTTCACAAGGAAGCATCAGTGCATGGAGTGTCATCCAGACATAT AGAACTACGAGATGGAACAGAGTTTAGCTTTCCAACGAACAAAAAGAAACCTTCATATCTCGATAGGTATCCAGACTTCA AAAAGCAGTACCTTGACTCTTTGCGTGAAAAGGATGAGCGTAAAGCTCTGAACCTGTTGCGCGGGTTTATCTTTTATATA ACGAATGTGGTTCGTGATGATGCATTCAAACCGTGGCTCGATCAAGAATGTTTGAAGATCAGATGTGTCTGACAAAAGAG TATCAGCACTTGGAGTTCTGACAGAAGACTACTAATTAGGAGATGTAAGACCTTTGTTTCATGAAATAAGTTTGGACAAG ATTTGCATTTG ................................................................................ .............N....................N.....................................i....... ....i.................i.......................................N...........N..... N...............................i..i...N........................................ ................N..N..N.....N..N..N..N................i......................... .................i............................................N...........i..... ...N............................................................................ ....N....................N...................................................... .....................................i...............N.......................... N................................N..............N............................... ................................................................................ ................................................................................ ........................N..............................................i...N.... ..........................................................N..................... ...........i.................................................................... .................................N.............................................. ....N...........N..N...........................N..............N................. ..........................................N.................N................... ........... Pos Score Pred ------------------------ 94 0.247 - 115 0.079 - 153 0.578 Yes 165 0.704 Yes 183 0.716 Yes 223 0.165 - 235 0.091 - 241 0.107 - 273 0.902 Yes 276 0.943 Yes 280 0.164 - 337 0.112 - 340 0.202 - 343 0.126 - 349 0.144 - 352 0.083 - 355 0.133 - 358 0.209 - 375 0.617 Yes 418 0.514 Yes 463 0.091 - 475 0.510 Yes 484 0.162 - 565 0.454 - 586 0.110 - 678 0.711 Yes 694 0.237 - 721 0.491 - 754 0.101 - 769 0.177 - 985 0.352 - 1032 0.694 Yes 1036 0.437 - 1099 0.309 - 1132 0.631 Yes 1234 0.191 - 1285 0.346 - 1297 0.323 - 1300 0.318 - 1328 0.308 - 1343 0.426 - 1403 0.073 - 1421 0.034 -

Explain the output -- Processing locus 634: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCAATAGTGGCACGAATAACAATATTACAAATAAAATAAAATAAAGAGCTTAAAAGAACAATCTGTCAAGAATCTTTGT GCTTCAATCTTTGAAAGCTAAAACAGTAATAATAGCTTCAGTAGCCAACAGCTCTTTGTCTGTACACTGCTGGTTTCAAA AGGATTCTCTTTTATACAAGCAAAAGAAACAAAGAAAAAAAATCAAAAGAGCTTAATCAAGAAAGATATGTTTGGCAATT GTGACCAGAACAAGAAGATGCCTATCATCAGCTCTCCCAACACCAATCCACTGGCGAGTATGCAAAGCAAGAACATGATC GTGGCTTCATCTCATCAGCAGCAACAACAACAGCAACCGCAGCAGCCACAACCGCAACTTAAATGCCCTCGTTGCGATTC TTCCAACACAAAGTTCTGCTACTACAACAACTACAGCCTCTCTCAACCACGGCACTTTTGCAAGGCTTGCAAGAGGTACT GGACGAGAGGTGGGACTCTCAGGAACGTTCCCGTAGGGGGTAGCTACCGGAAGAACAAACGTGTAAAGCGGCCATCAACC GCAACCACAACCACTGCCTCCACCGTCTCGACGACTAATTCTTCATCCCCTAATAACCCTCATCAGATCTCTCATTTCTC TTCCATGAATCATCATCCTTTGTTCTATGGTTTATCAGATCATATGAGCAGTTGTAATAATAATCTTCCAATGATCCCAA GCCGTTTCAGTGATTCTTCAAAGACTTGTTCATCAAGTGGTTTAGAGAGTGAGTTTCTCTCATCTGGTTTTAGCAGTCTT AGTGCTCTTGGATTAGGGCTTCCGCATCAGATGAGTCATGACCACACCATTAATGGTAGCTTCATCAACAACTCCACAAC AAACAAACCCTTTCTTCTATCGGGTCTATTCGGATCCTCGATGTCTTCTTCTTCTACTCTTCTCCAGCACCCACATAAAC CCATGAACAATGGTGGTGACATGTTGGGACAATCCCATCTCCAAACCCTAGCATCACTTCAAGATTTGCATGTTGGAGGT AACAATGAAGATATGAAGTACAAAGAAGGAAAGCTTGATCAGATCTCTGGGAACATCAATGGGTTCATGTCATCATCATC ATCTTTGGATCCTTCAAACTACAACAACATGTGGAATAATGCAAGTGTTGTCAATGGAGCATGGCTTGATCCAACAAATA ATAACGTTGGATCCTCCCTCACCTCTTTGATATAAACCAAAACTTTGGTTATATGATCTATAACTAGGTAGGGTTTATCT TTCTTTTTTTCCTTTTTGTTATGAGATGAGAGAAGAAGCTTGGATCAGAGAGAT ................................................................................ ................................................................................ ...................................................................N............ .................i.........................................N..............N..... ..............................................................i................. ................................................................................ ................................................................................ ................................................................................ ....i.....................N................i..........................i......... ................................................................................ ..............................i......N..............i........................... ........................................i....................................... ..i......i..........i................................................N.......... ....N.......i.............................................N.......i............. ............................i.........N..............N......N................... ....................................................N........................... ....................N....N............................ Pos Score Pred ------------------------ 228 0.396 - 258 0.595 Yes 300 0.384 - 315 0.483 - 383 0.587 Yes 645 0.506 Yes 667 0.309 - 684 0.692 Yes 711 0.573 Yes 831 0.517 Yes 838 0.244 - 853 0.739 Yes 921 0.690 Yes 963 0.568 Yes 970 0.843 Yes 981 0.802 Yes 1030 0.126 - 1045 0.353 - 1053 0.595 Yes 1099 0.276 - 1107 0.534 Yes 1149 0.599 Yes 1159 0.242 - 1174 0.245 - 1181 0.400 - 1253 0.101 - 1301 0.044 - 1306 0.074 -

Explain the output -- Processing locus 635: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTGAGCATAAACTTGCGGATTAGTTTGTCTTTTCGTGTGCTACAAGTCTTTTAGTCAAATTAGACGAATTTTACGCTT TCTTGTATACATTGATCCTAGAGTTCCGTAATTGCTATAAATAGATGTCAGTTTAGGAGTTACCAAATGCAAAAAAACAA AAAAAAAACACTATCATTAATCAAGTTCCACTGTTCATTTTCGATCTGCATAATGATGATGAGTCGTCGCTATGGAGCCA AGCTAATGGAAACTGCTGTGACTCACAGCCATCTTTTGAATCCTAGGGTTCCTCTCGTGACGGAAAATATTAGGGTTCCG GCGATGGGAGTCGTGAGAGTTTTCAGCAAGATGACATTTGAGAAGAAGAAAACTACGGAGGAGAAAGGATCTAGCGGTGG CAAGGCTGATCAAGGTAACAAAGGGGAGCAATTAATCGTTAGCTACTGGGGAGTGAAGCCGATGAAGATCACCAAAGAAG ATGGAACTGAATGGAAATGGAGTTGCTTTAGGCCATGGGAGACATATAAATCAGATCTGACTATAGATTTGAAGAAGCAT CATGTTCCATCGACTTTACCGGACAAACTAGCTTATTGGACCGTGAAATCTCTTCGATGGCCTACCGATCTTTTCTTCCA GAGGCGGTACGGATGCAGAGCAATGATGCTAGAAACGGTTGCAGCGGTTCCAGGAATGGTTGGAGGGATGTTAGTACACT GCAAATCGCTTCGACGGTTTGAACAAAGCGGTGGTTGGATCAAAGCCCTACTTGAAGAAGCAGAGAACGAGAGAATGCAT TTAATGACATTCATGGAAGTCGCGAAACCTAATTGGTACGAACGAGCTCTTGTGATTGCCGTTCAAGGCATTTTCTTCAA TGCTTATTTCCTTGGATACCTAATTTCTCCCAAATTTGCTCATCGTATGGTTGGATACCTTGAGGAAGAAGCAATCCACT CTTACACTGAGTTTCTTAAAGAACTCGATAATGGTAACATCGAAAATGTGCCTGCACCGGCTATTGCCATTGATTACTGG AGACTTGAAGCTGATGCGACGCTTCGTGATGTCGTCATGGTGGTCCGTGCTGATGAAGCGCATCACCGTGATGTTAACCA CTATGCATCCGATATTCATTACCAAGGTCGTGAGCTAAAAGAAGCTCCAGCTCCCATTGGATATCATTGAATCTGAAGAG CTTCTAGCTTTGTTTTGTCGTTTTGATACGGTCCAGTTTTGTTTTCCTTGTTCCTAAATGGGCTTTAATGGGTATTTGTT AAAATTTAG ................................................................................ ............................................i.....................N............. ....................................................N..i..i............N........ .....N.......................................................................... ...i..........................i................................................. .............................................................N.................. N.........N.....N.................N............................................. ............i.........i..N.............................N...........i............ ..........................................................................i..... ...N........N..................................................................N ..............................................i................................. ..............................N..............i.................................. .............N..............N.......i...............N.................N......... ..N............................................................................. .........................................................N.........N............ ......... Pos Score Pred ------------------------ 125 0.562 Yes 147 0.381 - 213 0.441 - 216 0.666 Yes 219 0.730 Yes 232 0.262 - 246 0.499 - 324 0.817 Yes 351 0.656 Yes 462 0.197 - 481 0.378 - 491 0.386 - 497 0.335 - 515 0.461 - 562 0.459 - 617 0.714 Yes 653 0.584 Yes 663 0.636 Yes 666 0.396 - 696 0.475 - 708 0.515 Yes 795 0.636 Yes 804 0.326 - 813 0.378 - 880 0.231 - 927 0.685 Yes 991 0.250 - 1006 0.517 Yes 1054 0.273 - 1069 0.333 - 1077 0.645 Yes 1093 0.258 - 1111 0.186 - 1123 0.106 - 1258 0.053 - 1268 0.056 -

Explain the output -- Processing locus 636: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAAAAAGAAACATCGAAAGCCCACGAATGGCCCAGTGGGTTTCTATGTGTTTCAATAGCCCACGAAAGCACCTCTACTA CAATTATATTCACCAGCCTAATATTCAACAATGACGATAATGTCAGATCTTCCACGGGATTTGTTAGCGGAGATTCTCAG TAGGGTTCCGTTGACATCTCTAAGAGCAGTGCGATTGACTTGCAAAAAGTGGAACGATTTATCCAAAGATCGGAGCTTTC TCAAGAAGCAAATCGTCGAAACAAAGAAGAAGCAATTGGAATCGAAGGAGATTGAGGTGATCATGATGAGGAATTTTAGG GTTTATCTAACAAGCATCGATATCCACAACAATGTTGATCCATCATTTACGCCTAAAGGTACACTTATTAGCTTAAGCGA TGATGCAAACCATCATCAAGTCGATAATGTATCTAGAGTCTTTCACTGCGATGGTTTACTATTATGCATCACCAAAGACC TCCACTATAGGCTTGTGGTTTGGAACCCTTATTTTGGTCAAACAAGATGGATCCAACCCAGAAATTCTTACCACAGAAAG GACAATTACGCTCTTGGGTACGATGAGAAGAAGAACCACAAAATCCTGAGATTGAAGGATAATTATTACGCTCCACGGGA ACGGATTTGTGAGTTTGAACTCTACAGTTTTGAGTCTAATTCATGGAAGGTTGTACTTGATGTCAGTCCTGACTGGTATA TACCTTCTTATAACCGTGGCTTGTCTCTAAAGGGGAATACTTACTGGTATGCAACAGAGAAGCATGTCAATGTTGATTTC TTGATTTGTTTCGATTTCACAACAGAGAAATTTGGACCGCGTTTGCCTCTGCCTTTTAACGCTACCGAGTCTCCTACTTA TGAAGATGTTGTTACTCTCTCTAGTGTTGGAGAAGAGCAGCTTGCGGTGTTGTTTCAGAGTGAGTATACATTGATGATGG AGATTTGGGTTACGAGCAAGGTTGAGTCCACAGAGGTTTTGTGGAACAAATTGTTCTTAGAGGTTGATTTGATAGCAATC AGCTCTCACTTTCAGTTTCTGGCAGAGGCTGGGAGTTTCTTCATTGACCAGAAGAAGAATGTTGTTGTGGTTTTTGATAA AGATATGGATGAGGCTACCGATCGCGACATGGCTTACGTTGTTGGGAAGAACGGATACTTCAAAAAAGTGGATATTGGGG AAGAAGCATACACGAGTTGTTTCCCACTTGTATGTTCTTATGTTCCAAGCTCAGAGCAAATCAGGCAGCTAACCTGATTA TTACGAACTACTGTTTTCTTTTTCTGTCATCTTCGATTGTATAAATCTGACCATTGTTATCAGTTATCTGGAATCTCAGA GACATGGCCTCTCTAC ...........................i.................N.................................. ..............................i........i........................................ ................................................................................ ..............................................................N..i.............. ...............................N...............................................i ..N.......................N.......................N............N................ ..............................................i................................. ......................N......................................................... ..........................................N................N.................... ................................................N..............N.....N.......... ...............................................................................N .....N...................................................................N..N... ................................................................................ ..........................................................N..................... ....i...N...................i................................................... ...............................N.......N........................................ ................................................................................ ...N............ Pos Score Pred ------------------------ 28 0.631 Yes 46 0.156 - 111 0.851 Yes 120 0.760 Yes 303 0.268 - 306 0.500 - 352 0.236 - 400 0.530 Yes 403 0.309 - 427 0.235 - 451 0.332 - 464 0.240 - 527 0.514 Yes 583 0.122 - 683 0.377 - 700 0.217 - 769 0.073 - 784 0.222 - 790 0.396 - 880 0.108 - 886 0.133 - 954 0.292 - 957 0.335 - 1099 0.128 - 1125 0.602 Yes 1129 0.162 - 1149 0.733 Yes 1232 0.229 - 1240 0.212 - 1364 0.090 -

Explain the output -- Processing locus 637: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGCGAGCATTACTTTATTATTGACTAGTAATTTTCAGAATCTTAAAACGAAATTTCTAATTAGTTTATTTAAAACCACAT TCATCATCTCTACCTATTTAGAAACATTCGTCCAGACTTCTTTCTTTGGTCTCGAAAAACATGGAGTTGGGAAAATCGAT TGAGAACCATAACGACGTTGTGGTAAGACTAACGAAGCATGTCATCGCTACCGTCGCCAATGGCTCCAATCTTGTCTTCT CACCGATATCAATCAACGTTTTGCTCAGCCTCATCGCGGCAGGTTCATGTTCAGTCACCAAAGAACAAATCCTCTCATTC CTCATGTTACCGTCAACGGATCACCTCAACCTAGTCTTGGCTCAGATCATCGACGGCGGCACCGAGAAAAGTGATTTGCG CTTGTCTATAGCCAATGGTGTTTGGATAGACAAGTTTTTCTCCTTGAAGCTTTCCTTTAAAGACCTTTTGGAGAATTCTT ACAAGGCAACTTGTAGTCAAGTTGACTTCGCAAGCAAGCCTTCTGAAGTGATTGATGAGGTGAATACATGGGCTGAAGTC CACACCAATGGACTCATCAAGCAGATTCTTTCACGCGATTCTATCGATACTATCCGTAGCAGCACACTCGTTCTTGCAAA TGCTGTATATTTCAAAGGAGCGTGGAGCAGTAAATTTGATGCAAACATGACAAAAAAAAACGATTTTCATCTTCTTGATG GTACCTCGGTGAAAGTCCCCTTTATGACCAATTACGAGGACCAGTACCTAAGAAGCTATGATGGTTTCAAAGTTTTGCGC CTCCCTTATATAGAGGATCAACGTCAATTCTCCATGTACATTTATCTGCCTAATGATAAAGAAGGATTAGCTCCTCTGCT TGAGAAGATAGGCTCCGAACCAAGTTTCTTCGATAACCATATTCCGCTCCACTGTATATCAGTTGGTGCTTTTAGAATCC CAAAGTTTAAATTCTCTTTCGAGTTTAATGCTTCAGAAGTTCTGAAAGATATGGGGCTGACTTCACCATTTAATAATGGA GGTGGTTTAACTGAGATGGTTGATTCACCTTCCAATGGTGACGATCTATACGTCTCAAGCATTCTTCATAAAGCTTGTAT TGAAGTGGATGAAGAGGGAACCGAAGCTGCAGCCGTCTCTGTTGGCGTCGTAAGTTGCACTTCTTTTAGGCGGAATCCCG ATTTCGTAGCTGACCGTCCATTTCTGTTCACTGTCAGAGAAGACAAGAGTGGAGTGATTTTGTTCATGGGTCAAGTTCTT GATCCTTCAAAACATTGATTGAACTGGGATGTGGAAGAAAACATTAAGCCACCATATGTTATGTTGTTGTATTTTGTTTA TATTCATGGTTTGATGGAAGTGGGTTCCTCTTTCTAC ................................................................................ ............................................................i................... ......................................N....................N.................... ..............................................i................................. ...i............................................................................ ..............N................................................................. ......................................................N............N............ .......N.......................................................................N ......................................N.......i..............................N.. .......................N.................................N..N................... .................................i..................N........................... ................................................................................ ...........................N......................i........................N.... ...............i..................N............................................. ........N....................................................................... .................................................................N.............. ............................N..........................N....N................... .....N.......N....................... Pos Score Pred ------------------------ 141 0.687 Yes 199 0.278 - 220 0.315 - 287 0.630 Yes 324 0.529 Yes 415 0.293 - 535 0.235 - 548 0.379 - 568 0.359 - 640 0.294 - 679 0.154 - 687 0.624 Yes 718 0.188 - 744 0.131 - 778 0.259 - 781 0.318 - 834 0.518 Yes 853 0.285 - 988 0.300 - 1011 0.588 Yes 1036 0.494 - 1056 0.882 Yes 1075 0.183 - 1129 0.281 - 1266 0.212 - 1309 0.043 - 1336 0.047 - 1341 0.074 - 1366 0.048 - 1374 0.043 -

Explain the output -- Processing locus 638: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGATTGGATCTGGAACTGGTAAGCTCTTCTATAAGTCTCTGGTTTTGTATCTTTCGAACTGTTCTTTTGTTTCTCTCTCC AAAAATTTCAAACTTTCATAATTTCGCCTCCTCAAGTTCGATTTCAGCTCAAGAACACGAATGCTTCACTCACTTGTTTC ATTGTTAAATCTTATATTTAGTTTGATACTCATGTTTTCTGCTGTTCGATTTTTTTTTTTCTCTGATTTATATTCGAGTT TGAACTTAAATCATGGTTTTGGTTTCACTTTACAATTTTGGATCTCATGCAGTTTCAAAACACTCTTCGATTTATATGAA TGTTTTATATTATTAGGGACATTGCTTGCAGACTGTGTCTTAAATCTTTTTGATTTTCTTGTGATCTTCAGAACTCAAAT TGGGAAGAATTTTGACATGGAGGAGGACCGGTGTTCGATCAAGGATGATGCAACTCAATTGATAGGTAACACCCCAATGG TATATCTGAACAACATTGTGGATGGTTGTGTAGCTCGTATTGCAGCTAAGCTTGAAATGATGGAGCCTTGCTCTAGTGTC AAGGAGCGAATTGCTTATGGTATGATTAAAGATGCAGAAGACAAGGGATTGATTACTCCTGGGAAGAGCACACTGATTGA GGCTACCTCTGGTAACACCGGGATTGGTTTAGCCTTCATCGGTGCAGCTAAAGGTTACAAAGTGGTCCTCACAATGCCCT CATCAATGAGCCTTGAGAGAAAAATCATTCTTTTAGCATTAGGTGCGGAGGTTCACCTCACAGATCCTAGTAAAGGCGTT CAAGGAATAATCGACAAAGCTGAAGAGATATGTAGCAAAAATCCAGATAGTATCATGCTAGAACAGTTCAAAAATCCTTC AAACCCGCAAACTCATTATCGAACCACGGGTCCAGAGATATGGAGAGACTCTGCAGGGGAAGTAGACATATTGGTTGCCG GTGTTGGAACTGGTGGAACGCTTTCCGGATCAGGAAGATTCCTCAAGGAGAAGAATAAAGACTTTAAGGTTTATGGTGTG GAACCTACAGAAAGTGCGGTAATAAGTGGAGGCAAACCGGGTACACATTTGATCCAAGGTATTGGGGCTGGACTCATCCC AGACAATTTGGATTTCAACGTTCTTGATGAAGTCATCCAAGTGACAAGTGTGGAAGCAATTGAAACAGCCAAACTTCTTG CCCTGAAAGAAGGATTACTGGTGGGAATATCTTCTGGAGCTGCTGCAGCCGCTGCGATAAAGGTGGCAAAGCGGCCAGAA AACGCCGGCAAACTCATAGTTGTGATTTTCCCTAGCGGTGGGGAACGTTACCTATCGACCTCATTGTTCGAATCAGTCAG ACATGAAGCAGAGAATTTGCCAATTCAATGAGGCTTTTACACGACCTGACTTGGTTCGTTTTGGTGAAAAAGTACCAAGA TTTCGGTTCGACATTTACACTGTCATAGTTTCATGAACTTTAAGATTTCA ................................................................................ ............................................................N................... ...............................N................................................ ............i.................................N............................N...N ................................................................................ ................i...........................N..N............................N... .....................N..................................i..i.................... ................N....N.........N................................................ .........................................................................i...... .....i.......................................................................... .............................i........................i......................... .......................................N........................................ ........................................................................N....... ................................................................................ ..........................N..................................................... ................................................................................ ................................................................................ ..N........................N.................................................... ................................N................. Pos Score Pred ------------------------ 141 0.148 - 192 0.266 - 253 0.551 Yes 287 0.151 - 316 0.205 - 320 0.168 - 417 0.738 Yes 445 0.222 - 448 0.084 - 477 0.413 - 502 0.315 - 537 0.536 Yes 540 0.748 Yes 577 0.101 - 582 0.418 - 592 0.386 - 714 0.860 Yes 726 0.611 Yes 830 0.594 Yes 855 0.679 Yes 920 0.478 - 1033 0.285 - 1147 0.242 - 1363 0.247 - 1388 0.283 - 1473 0.041 -

Explain the output -- Processing locus 639: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGTTTTCTCTTATTGATTTCTGTTTGCATGGGTCTGTTCTATGATCTAGTGTTGAAGAATTAGTGTCTAGTGTGCAAAA ATTGGAAGACTGGACAAATGAAAAGCTTTAAATTATAATCGCATAGCTCCATTATCAAGACACTGTCTCCAATTTTCATG TTTTTGTTTGTTTGATCTTATTTCTGGTATAATGCAGGGGCTTATTCGCTTGTATCTGTTGATATACAAGATGGCATCTG CGACTCTGAGAAGGCGGCTTCATCACGGTGATGTAGATGGGAGGAAGTATGAACGCTATGATGCAACAGACTCTGAAACT TTAAGTGAACCCTTGTTAGGAAGTAGTAGTACTGATAATAGTAGAAATGAGTACATTGAGGAGCGTACTCTGGAGGATAT TTGGGAAGAAGAACGGAAGAGACAGCAAGTTCACTGGACGTTAATCTTTTCTCAGTTGATTGCACAGTGGGCTCAGTGGA TAGCCAAAATAGTCTTTGGATCTGGCTCACTTGTTGGGAGGTTTCTTTCTCTGCCTACATTTGGTCAAATAGGTACTGGT GGGAGGCTTCTGCCACCACCTCTTAGTATGTTGCAGGAAGAAAGGCTGAGAAATATTAAGCGAAGAATAGAAATCCCATT TGATGGATCTCGAATGGAGCATCAAGATGCTCTTAGACAACTATGGAGGCTAGCTTATCCACAGAGAGAACTACCACCTC TTAAATCTGAACTCTGGAAGGAAATGGGTTGGCAAGGAACAGACCCTTCAACAGATTTTCGAGGTGGAGGGTATATATCA CTGGAGAATCTAATCTTCTTTGCGAAGACCTATCCGGAATCATTCCAGAGATTGTTACATAAACAAGATGGAACTAGAGC GGAATGGGAATATCCTTTCGCGGTGGCTGGCATCAATATCTCATTCATGCTGGCACAAATGTTAGATCTTCAATCAGGTA AACCATCAACAATAGCCGGGATAAGATTCTTGGGATTTCTGGAAGAAGACGAAATGGCATTCGATAATCTCTACTGTATA GCTTTCCAAATGATGGATGCACAATGGCTTGCTAGACGAGCTTCTTACATGGAATTCAATGACGTCCTGAAATCGACGAG AGCACAATTGGAACGTGAGCTGGCACTGGATGATGTTTCAAGCATCACAGACTTGCCTGCTTTCAATCTTTTGTACAAAT AAATTGTTAAATTTCCTACAATTTCTATCTGTATTTAGATAATAATTATCTGAGAAATTCAGTGTAACAAATAATCAGTT TTTGCTGAAATTGCTCGGCAA ............................N............N...................................... .................N...........................................................N.. ...............................i......................................i......... ..............................N.....N...........N........N..N................... ..............................................i................................. ................................................................................ ................................................................................ ...........................N.................................................... ..i..........N............N...............N..................................... .......................i........................................................ ...................................................................N............ ...N..........................................N...........N..................... .....................................................i.......................... .........i..i...N......i........................i.........N..................... .............................N..N............................................... ................................................................................ ..................... Pos Score Pred ------------------------ 29 0.401 - 42 0.282 - 98 0.180 - 158 0.332 - 192 0.593 Yes 231 0.756 Yes 271 0.293 - 277 0.365 - 289 0.404 - 298 0.106 - 301 0.114 - 367 0.535 Yes 588 0.484 - 643 0.610 Yes 654 0.445 - 667 0.324 - 683 0.445 - 744 0.588 Yes 868 0.439 - 884 0.444 - 927 0.366 - 939 0.224 - 1014 0.676 Yes 1050 0.600 Yes 1053 0.544 Yes 1057 0.222 - 1064 0.768 Yes 1089 0.731 Yes 1099 0.327 - 1150 0.153 - 1153 0.207 -

Explain the output -- Processing locus 640: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTACACACAAGTCATCACTCAAATTATATCAAAACATATTTTAAAAATATAAGCAGACAAAACTACTACCGTAGCAAAT CCTAGCTACCACAATTATTGACAAATAAACAAACTAATGTTGGTTGAAAACGCTGCATTTTGCAAAACAATACAAATCAT AGTGATGATGTTATATATAGTAGAATCATTGTTTGTAATGGATGTGAAAAAGATGGAGATGAACTTGAGAATTGAAGACC TAAACGAAGAAACAAAGACTCTAATCTCTTCACTTCCTTCAGACAAAGATTTCACTGGGAAAACTATTTGCAAGTATCAA GGATGTTGGTATACTCACAATGTTCTTCAAGCTGTCCTCAATTTCCAGAAAAGCTTCAAGCCTCAAGACACCGATATCAT CGTTGCTTCGTTCCCTAAATGCGGCACCACTTGGCTCAAGGCGCTTACATTCGCACTCCTTCATAGATCAAAACAGCCTT CTCATGATGATGATCATCCTCTTCTTTCTAATAATCCACACGTTCTTGTACCCTACTTTGAGATAGATCTCTATCTACGT AGCGAAAATCCTGACCTTACCAAGTTCTCATCATCTCCGAGGCTGTTTTCGACACACGTGCCGTCACATACGTTGCAAGA AGGTCTCAAAGGTTCTACTTGTAAAATTGTGTATATATCTAGAAACGTAAAAGACACATTGGTTTCATATTGGCATTTCT TTACTAAGAAACAAACCGATGAAAAAATAATAAGCAGTTTCGAGGATACGTTTGAGATGTTTTGTAGGGGAGTCAGCATT TTCGGGCCTTTTTGGGATCATGTCTTAAGCTATTGGAGAGGAAGCTTGGAAGATCCAAACCATGTGCTTTTTATGAAGTT TGAAGAGATGAAAGCAGAACCTCGTGACCAGATCAAGAAATTTGCCGAGTTCTTAGGTTGTCCTTTTACTAAGGAAGAAG AAGAGAGCGGATCGGTGGATGAGATTATCGATCTTTGTTCTCTACGTAATCTGAGCAGTTTGGAGATCAATAAGACCGGA AAATTGAATTCTGGTAGAGAAAACAAAATGTTTTTCCGTAAAGGAGAAGTTGGTGATTGGAAGAACTATTTGACTCCTGA AATGGAGAACAAAATCGACATGATCATTCAAGAGAAACTTCAAAACTCTGGTTTGAAATTCTGAGTTTGTGCATTGTAAT AATAAGACTACCTCTTATGCTGTTGTTCTATTCCTTTTGATGTAATAATGATTATGAATAATGGTCTTTGTTTTCGTTCA CTC ................................................................................ ....................................i........................................... ....N..N.............................i...N..........i.....N..................... ................................................................................ ..N................N............................................................ ..................N............................................................. ...N..N..N...................................................................... ................................................................................ ................................................................................ ..................i.....................................N....................... ...................N.........................................N..........N....... .......i........................................................................ ..................N............................................................. ...........................N.................................................... ................N......................N.......N.....N......N................... ... Pos Score Pred ------------------------ 117 0.623 Yes 165 0.488 - 168 0.378 - 198 0.570 Yes 202 0.439 - 213 0.676 Yes 219 0.272 - 323 0.301 - 340 0.313 - 419 0.160 - 484 0.303 - 487 0.160 - 490 0.207 - 739 0.518 Yes 777 0.346 - 820 0.147 - 862 0.247 - 873 0.424 - 888 0.697 Yes 979 0.107 - 1068 0.441 - 1122 0.380 - 1140 0.098 - 1217 0.041 - 1240 0.041 - 1248 0.068 - 1254 0.070 - 1261 0.116 -

Explain the output -- Processing locus 641: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTATAGATTATTTACAGTTATCTCTATAGTATTATTTACATTTATATTTATTTTAAATTATGAAACTTAGATTAATTTG TTCTCTATATAAATAATTTAGACAATTATTCTTATGGGAGTCTTTTTTTTTTTTTCACTGAAATGTTGCCTTTCATTGAT ACCAAATTCTTATGGGAGTCTAACAGTAATTATTCATAAATACTTTTATCTTCGTACTCCCATAATTTATCGAACCATAT TGGGCCGTGGGAAAAGCCGATTAGTCATACAAAGCGTAAGAAAAAAAAAAGTTTCATTGTGTTGTGCCATTGTTTCACTT TCTCTCGCAATGGCGTCATTGAAGATGATAGGCAAATCAGACATAGCCGTTAACTTCACAGATGGGATGTTCAAAGGACT TTACCATGGCAAGAACTGTCATGTTCCGGATATTGCCACTGTCTTGAACCGGGCTTGGTCTGCTGGAGTCGATAGAATCA TAGTAACTGGTGGGTCATTGGAAGAATCAAGAGAAGCTCTTGCAATTGCAGAGACTGATGGTAGGCTGTTTTGCACTGTT GGTGTTCATCCAACTAGATGCAATGAGTTTGAAGAGAGTGGTGATCCGGAGAAGCATTACCAGGCTCTTTTTTCATTAGC CAAAGAAGGAATGCAGAAAGGGAAGGTGGTAGCAATTGGTGAATGTGGATTGGATTATGACAGGCTTCAATTTTGCTCAG TTGATATCCAGAAGAAGTATTTTGAGAAGCAATTTGAACTAGCATATGCTACAAAGTTGCCAATGTTCCTACACATGCGA GCAGCTGCTGAAGATTTCTGTGAAATTGTTGAGAGAAACAAGAATAGGTTCACTGGAGGAGTTGCTCATTCATTTACTGG TAGTGCGTCGGACCGCGATAAGCTTCTCTCTTTCGATAAAATGTACCTTGGTGTGAACGGGTGCTCGTTGAAAACAGCTG AGAATCTTGAGGTAATGAAAGGAATACCAGTAGAGAGAATGATGATCGAAACCGACTCTCCATATTGTGATATCAAGAAC ACACACGCCGGAATCAAGTTTGTAAAGTCGACTTGGCCTTCCAAGAAAAAAGAGAAGTATGATCAAGAGTCCCTTGTCAA AGGCCGTAATGAGCCTTGTTTAGTCAGGCAAGTCCTTGAAGTGGTGGCTGGCTACAAAGGACTTGGCGATCTAAATCAAG TTAGCTCAACCTTGTACCACAACACTTGCAGGGTCTTCTTCCCTCAGGACTTAGATTCTGCTGCAGATGCTCTGCTTTCC GGGCACCACGAGACCGACTAGCATTAATCGTGACAGAAATTTGCAGTTTACTGGAATCAAGATATGTTGTTACATTACAC CATTTATGGATTAAAAACATTCTCAATAAAAATTACAAAC ............................................................N................... .................................N............................N................. ...........N.................................................................... ................................................................................ .........i..............i....................................i....i............. .....i..............N........................................................... .........................................................N...................... .................N....N......................................................... ..........N...............................i.............N....................... .............................................N................i...........i..... ................................................................................ ........................................N....................................... ..............N.......................i..i...................................... ..........................................................N..................... ........N....................................................................... ..................................................................N............. ...............................................................N................ .....N.................................. Pos Score Pred ------------------------ 61 0.123 - 114 0.251 - 143 0.476 - 172 0.399 - 330 0.748 Yes 345 0.659 Yes 382 0.509 Yes 387 0.690 Yes 406 0.533 Yes 421 0.265 - 538 0.194 - 578 0.197 - 583 0.332 - 651 0.396 - 683 0.506 Yes 697 0.202 - 766 0.148 - 783 0.645 Yes 795 0.724 Yes 921 0.452 - 975 0.495 - 999 0.614 Yes 1002 0.521 Yes 1099 0.485 - 1129 0.362 - 1267 0.201 - 1344 0.122 - 1366 0.052 -

Explain the output -- Processing locus 642: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCAACTCAAATAGCTTCACAGTTTTACTAATCTCTCTCATCTTCTTCCCAACATCAAAGAAGACCTTTACTTCTGGTT AGAATAGACCAAGAACACTGAGTACGACAAAGAACCATGATGAATCTTTCTTCTCGTCGCGCCTCTTCCACTCTCACATT GAGCTTCACTGTTCTCCTTTTAGCTTCCACAGGTATAACCAGAAGAAGAAGAAGAGTCTCTCACTCACTTACTTCAAAGT CTATCACATCTACTTACACTCGTTTCAAAGTTTCATTTTTTTTAGTGATTTGTTTCTTTATCTTTTGGATGTTAAAAGGT TCGTATGGCTCAACATTCACATTCGCTAATCGGTGCGGCTACACCGTATGGCCTGGAATCCTATCGAATGCTGGCTCACC TACTCTCTCCACCACCGGATTTGAGCTCCCTAAAGGCACCTCTCGCTCTCTCCAAGCTCCCACCGGTTGGTCCGGTCGAT TCTGGGCTCGTACCGGTTGCAAGTTCGACAGTTCTGGCTCCGGTACATGCAAAACCGGTGACTGCGGCTCCAACGCTGTC GAATGCGCCGGGCTTGGTGCCGCACCGCCTGTAACTCTCGCGGAGTTCACTCTAGGCACTGGCGGCGATGATTTCTACGA CGTTAGTCTCGTCGACGGTTACAACATTCCGATGATAGTTGAAGTCGCCGGTGGATCTGGGCAGTGCGCTTCTACCGGAT GCACTACGGATCTTAACATTCAGTGCCCTGCTGAGCTGCGTTTCGGCGACGGAGACGCTTGCAAAAGCGCGTGTTTAGCG TTTCGGAGTCCTGAATACTGTTGCAGCGGCGCGTATGCTACACCTTCTAGTTGTCGGCCTTCCGTGTATTCGGAGATGTT TAAAGCCGCGTGTCCTCGCTCCTATAGCTACGCTTACGACGATGCTACGAGCACTTTCACTTGCGCCGGTGGAGATTATA CGGTGACGTTTTGCCCTTCTTCCCCTAGTCAAAAATCGACTAGCTACTCCCCTCCAGTGACAGACTCGTCGTCGACCTCA CAAGGTTCCGATCCTGTGCCTGGTTCTGATACTGGTTACGCTGGTCAGGGTCAACAGACTCCAGGTCAAGGTAATGTGTA CGGGTCACAGGGGACTGGGTCGGAGATGGGTACGGGTGAGACCATGTTACAAGACGGATCATGGATGGCTGGTTTGGCCA TGGGAGAGGCAAGCAGACCAGCCGGCGTTTCATTGACAGTGCTGCTTGCTGCATTTACTTTTCCGTTTATTTTCTCGTAG CATCATCGTCTTTAGATTTGGTAGTCGTTTTGTTAGCATTGCCTTTGTTTAGTTTTTGTTGATACTCGATGATTATCGCT TAAGACTGCGACAATTTGG ................................................................................ ....................................i..i........................................ ................................................................................ ....................................................................N........... ....N..........................................N...................i............ ................................................................................ ..............................................N................................. ..N................................................................i............ ...............................i..............................................N. ................................................................................ ..................................N........................................i.... .........................................N...................................... ................................................................................ .........................................................................i...... .........................i.................N................i...i..............N ................................................................................ ....................................................................N........... ................... Pos Score Pred ------------------------ 117 0.686 Yes 120 0.682 Yes 309 0.369 - 325 0.455 - 368 0.474 - 388 0.864 Yes 527 0.212 - 563 0.202 - 628 0.603 Yes 672 0.728 Yes 719 0.156 - 835 0.387 - 876 0.774 Yes 922 0.423 - 1114 0.501 Yes 1146 0.845 Yes 1164 0.220 - 1181 0.537 Yes 1185 0.670 Yes 1200 0.280 - 1349 0.043 -

Explain the output -- Processing locus 643: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGTATATATAATTTTTTTCGTCTATCCAAAATTCTCCTAATTAATAACACTAATGTTTATGACTTATGAGTCTATCACT TAACACTAATTACGTTTTTAACCCTTTGTAACCCTAATTGGCTCCTGCTTCATGTTGTATATAACTGCTCTCAGAAACCT AAGTCGTCACTCCCTCTCCCTTGGTTCTTTCTTCCTCTTGAAAGCTAGAAAGAAGAAAATGGAAGAAGCTCTTAGCGAAG CTCTTAGCAGCTTTGACTTCGATGAACTTGTAGCTTCGCTTGCTGATGCCATCGCAGCAACATCGACTCCCAAGCCTTTG GTGGAATTTTCACTACATGCTTTGATGGCTGAATTGTTTAAGAGAACTAAAACCTTACCCAAAGCTGAAGTCAGCAGGAC ATTCTTCAGAGCTGTTTCCATTGGCTTGCTGAGAGGTAACATTAGGAAAGATCAACTGGAGAGAACTTGTGATGCTGGTA AAGCAATTATCAAAAGAGTTGCTGCAATTCTAGGGATTGAAATCAGAGACAAGGGTCAAAGAAAGCTTGTTCTAACAAAC AAAACTGTGACTTTCTCAAGAATGATAGCTATTTTCCCTCACTGGGCTAGCCAGCTAATGGTCTCTAAGGAGAGCCTTTT TGTCAACAGGTTTGCTTCTTTCAATGATGCCAATAGATTTCCTGCTCAAACTCATAAGCTTCCTATTGCTATGCTTCACT CTGGATTTGCTGGTCTGATTCCAAGAGAAAGTGATGATATGGAATTCATAGACCTTATTTGCATGTGTCACTCTGCTTTC ATGGCTGAGTTCTCTCTTCTGATTACTCCTCAAGAACAGAGAAGTTTTCATGCATTGTACGAGAATCAGAAAAGATATAC CGAGGCTGCTCGTGGATCTGGATTCAATTCTGATGAAAATAGGATCGTTTATCTTTTGAGATTTGGGTTGTTGTCTTCTG ATAGTCTTGATAAGATGTTGAAAGTTGCTGCAAGGGTTTCAACTTTGAGTGCAAGGCAAGAAGAAGCAAGTGTTACAAGA GACAGCTTTACGATCGTTGAAGATCGGGTCAAGATCGTAAATGATGATGCAAAGTGGGATGAGCTATTGAAAAAAGCCAA GAGAATAGTTGATGCTTCAAGTTCAATGAGGTCTCAGGTTGCTGCTGTTGACTCCTTAGATGGAGTTTGATTTAGTGCTT TATTGAGCTTCTTTCTTTTGTTTCGTTTGATCTGTTCTTAAGTATAGACTTTCTAGTCTTTCTTTTAAGTTTCTTTAGCT ATTGCCATT .....................................................N.....N......N............. ...................................................N............................ ..........................................................i..................... .....................N.......................N.................................. ................N.......N....................................................... .......................................................................N........ ................................................................................ .....................i...................................i...................... .......................N..N...........................................N......... .................................N....N.......................i................. i................................................N.............................. ................................N............................................... ..............i................................................................. ........................................N..N..N...........N..................... ...........N.............N.................................N.................... ................................................................................ ......... Pos Score Pred ------------------------ 54 0.249 - 60 0.200 - 67 0.374 - 132 0.494 - 219 0.909 Yes 262 0.108 - 286 0.120 - 337 0.227 - 345 0.319 - 472 0.223 - 582 0.540 Yes 618 0.682 Yes 664 0.376 - 667 0.196 - 711 0.367 - 754 0.243 - 759 0.386 - 783 0.617 Yes 801 0.853 Yes 850 0.326 - 913 0.056 - 975 0.646 Yes 1081 0.162 - 1084 0.165 - 1087 0.134 - 1099 0.136 - 1132 0.127 - 1146 0.371 - 1180 0.134 -

Explain the output -- Processing locus 644: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCTACCAAATACTGTTTGTTCCCCATAGCCACACTCCATAGACATAACAAACAATAAACTATGGAAAAAGATAGAATTTG ATGTGATAAAACATTCATTAATTGGATCTATATATATCTTTCATGCCTCCACAAAAAGAAAACCATAGAACATTGAACAA AATGAAGACTAATCTTTTTCTCTTTCTCATCTTTTCACTTCTCCTATCATTATCCTCGGCCGAGCAATGTGGTCGCCAAG CTGGAGGAGCACTCTGCCCCAACGGTCTATGCTGCAGCGAGTTCGGCTGGTGCGGTAACACCGAACCATACTGTAAGCAG CCTGGCTGCCAAAGCCAGTGCACTCCCGGTGGTACTCCTCCTGGACCCACCGGCGATCTTTCGGGCATCATTTCAAGTTC TCAGTTCGATGATATGCTTAAGCATAGGAATGATGCCGCTTGTCCTGCTAGAGGTTTCTACACTTACAACGCCTTTATCA CCGCTGCAAAGTCCTTCCCCGGTTTTGGCACCACCGGAGACACCGCCACGAGGAAGAAGGAGGTCGCCGCCTTCTTCGGC CAGACTTCCCATGAAACTACAGGTGGATGGGCTACAGCACCAGACGGACCATATTCATGGGGCTACTGTTTCAAGCAAGA ACAGAATCCTGCTTCAGACTACTGTGAACCGAGCGCCACGTGGCCATGCGCATCTGGCAAACGCTACTACGGAAGAGGAC CAATGCAACTGTCGTGGAACTACAATTACGGTCTATGCGGTAGAGCAATAGGAGTTGACTTACTCAACAACCCTGACCTT GTTGCCAACGACGCAGTGATCGCTTTCAAAGCCGCGATTTGGTTCTGGATGACTGCTCAGCCTCCCAAACCGTCTTGCCA TGCCGTGATAGCCGGCCAGTGGCAGCCTTCAGACGCCGACCGTGCCGCCGGGAGATTACCGGGTTATGGAGTGATTACGA ACATCATTAACGGTGGATTGGAGTGTGGACGTGGCCAAGACGGGAGAGTCGCCGATCGTATAGGGTTTTATCAGAGGTAT TGTAACATATTTGGTGTTAATCCTGGTGGTAATCTTGATTGTTACAACCAAAGATCCTTCGTTAACGGCCTCCTCGAAGC TGCTATTTAGTAACGAGAGTATTATTATTATAATATAATACGCAGCTTTGTATTATCAAAACAATAATATCAGACTAATA AAACTCTTTCCAATGATGTTTCACTGTTT ............................................................i................... N.........................................N..................................... ............................N................................................... ................................................................................ ........N....i...............N..i............................................... ................................................................................ ..........N...............N.............................N....................... .............................................N.................................. ..i...............................N............................................. ................................................i..............................N .................................................................N.............. ................................................................................ ................................................................................ ................................................................................ ............N..N............. Pos Score Pred ------------------------ 61 0.626 Yes 81 0.275 - 123 0.448 - 162 0.653 Yes 227 0.572 Yes 269 0.320 - 409 0.139 - 414 0.763 Yes 430 0.330 - 433 0.671 Yes 571 0.477 - 587 0.307 - 617 0.350 - 686 0.368 - 723 0.783 Yes 755 0.251 - 849 0.707 Yes 880 0.301 - 946 0.319 - 1213 0.064 - 1216 0.043 -

Explain the output -- Processing locus 645: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTTTTAAATTGTATATTAACTTTATGTTTTATTATATTCCAAACTATAACTTATAATTAAATGTAAAAAACTAAATAT TTGTGAAATGGAGAAAAGTAAACTATTATCATGACATATGTCTTCATTTCCCTTAACCTTAATCTTAACATAGTAAAAAT CTCCTTCTGTGAAATCTATATATTTTTTAAAGGGAGCAATATAAAATCTTACTGTGATATATATATATATATATATATAT ATATATATATATATATATATATATTTCTTCATTATTCCTTAACTTATGATTCTTCAATTTTACGTTTGAATCTAATGATG GCAGATTGGTCCTTGCTTCCGAATGACTTACTAGAACTAATCGTGGGTCATCTTGAGACATCTTTCGAGATTGTCCTCTT CCGATCCGTCTGCAGCTCATGGCGGTCTGTTGTACCTCCACAAGATCAATCCCGCTGCTTAAGTATCAAAACTCATGATA TATCATTCAATGCCGGGTTTTCGTTCAGCGGCCAACCAACGGATCATCCTCTGTTTGGAAGCACACTAACAAAGATCCCA ATTTATCTAGTGAAATTCTGGACTCCGTTTGGTGATGATTATTTATTGGCAGAGATGCGTGAACGAGAGGGTGGGGAACC GATGTTTTTGTTGTCGCCCTTGTCATCTAATCGTATTATATATGGAATGGGAATAAATAAAGTGTTGTTCAACTCTCTTA CCTCACCAATTATCCCATTTGGACAATACTACGAGATAACCTACTCTGAGAAGCAACCCATTCGTTACCGCTATGGATTA CCCTGTCATTTATGGGATAAATTAGAGTGGGTGGAGATTACCGAGAGAGTTGAATTTCTTAAACTGGATAGTGAAGATAG CAGAGATTTTGCGGTGCTTTTTGCTGGAAGAATGTGCAATTTGGTGATGTATAGAAGCCGTAACATGAGTTGGACTCAAG TTGTGGAGCATCCTGAAAAGTATGCATATCAAGATTTGGTTGCATTCAAAGGAAAGTTTTATGCTCTTGATTCAAGTGGA AGGGGACGGGTCTTTGTTGTCGAACTTTCTTTGGAAGTTATGGAGATCCCCTCAGTCAGAGGATCACAACAGTCTTCTAA AGAAAATCTGGTGCTGTCAGGCGAGGAGTTGTTGCTAGTGCAACGGTTCACCCCTGAAGTGCGGCATTATGATGAATATC TATATACATGGTTCAGAGTGTTTAGACTTGATGAAGAGGAGGGAAGAGAAAGTGGGTTCAAGTTAATGACTCTAACGACC GAGTGATCTTTCTTGGAGCTCAGACGAATCTCTGCTGCTCGGTACATAAGCTCCCATGTGCAAAGGAGAATTGCATTGTG TTCATTGAATCAAATAATTGTTTCA .........................N....................................N................. .......N......................N......N.......................................... ................................................................................ .............................................N............................i..i.. ......................i......................................................... ..................i.......................................................N..... .........i...................................................................... ..................................N...................N......................... ........................................................................N....... ...........N.................................................................... ...............................N..............i.................i............... .....................N......................................N................... .......................................N........................................ ....................................................................N..N........ .......N......................N..................................N.............. .......................................................N........................ ......................... Pos Score Pred ------------------------ 26 0.243 - 63 0.314 - 88 0.126 - 111 0.492 - 118 0.219 - 286 0.106 - 315 0.596 Yes 318 0.613 Yes 343 0.511 Yes 419 0.535 Yes 475 0.185 - 490 0.505 Yes 595 0.160 - 615 0.479 - 642 0.156 - 682 0.276 - 687 0.426 - 793 0.311 - 812 0.165 - 912 0.387 - 927 0.683 Yes 945 0.627 Yes 982 0.112 - 1021 0.125 - 1080 0.425 - 1189 0.329 - 1192 0.249 - 1208 0.296 - 1231 0.344 - 1266 0.232 - 1336 0.062 -

Explain the output -- Processing locus 646: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAGTTCATCATCATCTAAAAGATCAATCTTTCCTGCTAGAATTTGTCGACCAAGTAAGATTCAACTCCAACCTTTCATT TAGATGTGCCCCTAAGCCTGAATTATGAACAATCTGTTTAATCTTTGCAATGAAGGAAACAAATGTGAAATCAGCAGAAG GGATTTTGCAATTAGGGGAGGGATTGTTGCTTCTGGAGTTTCAGTCATGGATACTTCTTCTGCTTCTTCTCAGTCTGTTC AAGGTAGTGAGAGGTTGGCTTTTAAACCAGAAGGCTATAACTTTTGGGAATGGAGAGGTCACAAGATTCATTATGTTGTT CAAGGAGAAGGCTCGCCTCTAGTTCTTATTCATGGTTTTGGTGCTTCTGTCTTTCATTGGAGGTATAACATTCCTGAATT GGCTAAGAAATACAAGGTTTACGCCTTGGACTTACTCGGTTTCGGATGGAGTGACAAAGCTCTCATAGAGTATGATGCCA TGGTTTGGACAGATCAAGTTATTGATTTCATGAAAGAGGTAGTGAAAGAACCAGCTGTTGTCGTTGGAAACAGCCTTGGG GGATTTACGGCATTGTCAGTTGCGGTTGGATTACCCGAGCAAGTCACGGGAGTTGCACTTCTGAACTCTGCGGGACAATT TGCAGCCGAGAGTAGAAAACGAGAGGAAGCTGATGAAACTGTGATAACCAAGTTTATAGTTAAGCCGCTAAAAGAGATTT TCCAACGTGTGGTTCTTGGGTTCTTGTTCTGGCAAGCCAAGCAACCTTCTCGAATCGAATCTGTCTTAAAGAGTGTGTAC ATAGACTCTACTAATGTCGATGACTACCTCGTCGAATCCATTTCAAAACCTGCAACAGATCCAAATGCTGGAGAAGTATA CTACAGATTGATGACAAGGTTCTTAACAAACCAGAGTAGATACACTCTAGATAGTGTTCTAAGTAAGATGACTTGTCCGC TTCTGTTGGTTTGGGGAGATCTTGATCCGTGGGTCGGTCCAGCTAAAGCCGAGAAAATCAAAGCATTTTACTCGAACTCA TCGCTCGTTCACTTACAAGCAGGGCATTGTCCTCATGATGAAGTCCCGGAAGCTGTAAACAAGGCTTTGTTGGATTGGTT GTCCATTAATATTGCTTCAAAGCCAGCTTCTCCTATAGTCTTGGAGACGTAAAAACTCTTTTTGTGAAGGAAGTATATAG TTGTAAATACATTGTTACCTTTTACAATTGCTGCTTCTGTGCTTGTGTTATCTGAGATTTAGCAGACTAAA ................................................................................ ...N....................i........................N............N................. ..............................................N................................. .................................................N......................N....... ...............................N................................................ .............................................N.........................N..N....i .............................i.................................................. ................................................................................ ................................N............................................... ................................................................................ .............i.....i............................................N............... ..........N........................................................i............ ................................................................................ ..................................N..N.......................................... ................................................................................ ....................................................................... Pos Score Pred ------------------------ 84 0.389 - 105 0.572 Yes 130 0.191 - 143 0.191 - 207 0.450 - 290 0.315 - 313 0.189 - 352 0.338 - 446 0.233 - 472 0.149 - 475 0.174 - 480 0.883 Yes 510 0.648 Yes 673 0.203 - 814 0.652 Yes 820 0.547 Yes 865 0.395 - 891 0.332 - 948 0.623 Yes 1075 0.103 - 1078 0.088 -

Explain the output -- Processing locus 647: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGCAATACTCTTTTTCGTTAATTATCTACCTTTAGTCCTTATAAATTTATAAGAGTACTCCACCAAACATAGTATTACC CGAATACCCGTTGGTGCCTAATACATGCACGTGATAACCAATTTTAATTTGCCAACAATTCTCTCTCTTGTGTGCAGTGT GTATATAAATATAGCCACACATACATACACTAATGTTGAAAATTTCTTCCTTATCATCTTCTCTCATAAAATCCCTCTCT CCCTTTCTCTAAAAATGGTGAAAGGGTCCCAGAAAATCGTGGCCGTAGATCAAGACATACCAATAATAGACATGTCGCAG GAGAGATCACAAGTGTCGATGCAGATAGTCAAAGCCTGCGAGAGTCTCGGCTTCTTCAAAGTCATCAACCATGGCGTTGA CCAAACCACCATCTCAAGAATGGAGCAAGAGTCTATAAACTTCTTTGCTAAACCGGCTCACGAGAAGAAATCTGTCCGAC CAGTTAACCAGCCTTTCCGGTATGGTTTTAGAGACATTGGACTCAACGGTGACTCTGGTGAGGTCGAGTATTTGCTGTTT CACACTAACGACCCTGCCTTTCGCTCTCAGCTCTCCTTCAGCTCGGCAGTGAATTGTTACATAGAAGCAGTTAAGCAGTT GGCTCGTGAGATCTTAGATCTGACGGCTGAGGGACTTCATGTCCCACCTCACAGTTTCAGTAGGTTAATCAGCTCCGTCG ATAGTGACTCCGTTCTGAGAGTGAATCATTATCCACCGTCCGATCAATTCTTTGGTGAAGCCAATCTTTCTGACCAATCT GTGTCACTGACAAGAGTTGGCTTCGGAGAACACACCGACCCTCAGATTTTAACAGTTCTTAGATCTAACGGTGTAGGAGG GCTCCAAGTGTCCAATTCAGATGGCATGTGGGTTTCTGTCTCCCCTGACCCTTCAGCTTTCTGCGTCAATGTAGGAGACT TGTTACAGGTGATGACGAACGGGAGATTTATAAGTGTAAGGCATAGAGCATTGACCTACGGAGAAGAAAGCCGGCTATCC ACGGCGTACTTTGCCGGACCACCGCTTCAGGCGAAGATTGGGCCTCTTTCGGCGATGGTTATGACGATGAATCAGCCACG GTTGTACCAAACATTTACTTGGGGCGAGTACAAGAAACGTGCGTACTCTCTACGACTTGAGGATAGCCGTTTAGACATGT TTCGTACATGTAAGGACTAGTATTCAGGTTTATGGCAAAGCTTTTTAGTAATATCTATGAAATGTTTTCGTCTAACTATG TTCCCTTAAAAAAGTTTAAGCTAAACGTAGACATGTTAG N............................................................................... ........................N....................................................... ................................N............................................... ..............i........................................................i........ ..................N...................................................N......... ...................N............................................................ .....................N.......................................................... ................................................................................ ......................................N......................................... ................................................................................ ................................................................................ ....................N....i..........................................N........... ...........i.................................................................... ......................................................N.....N.....N............. ............................................................................N... .......N.......................N........................N....N...............N.. ................................N...... Pos Score Pred ------------------------ 1 0.267 - 105 0.415 - 193 0.259 - 255 0.704 Yes 312 0.656 Yes 339 0.445 - 391 0.396 - 420 0.425 - 502 0.336 - 679 0.240 - 901 0.257 - 906 0.687 Yes 949 0.382 - 972 0.709 Yes 1095 0.218 - 1101 0.224 - 1107 0.160 - 1197 0.200 - 1208 0.200 - 1232 0.101 - 1257 0.076 - 1262 0.118 - 1278 0.048 - 1313 0.084 -

Explain the output -- Processing locus 648: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATTATTCTCGTTTTTCTGTCATTTGACTGATTAAATTAGTTGCAAGACATATCAGAAACATATAGAACAAATGTTAG ATCAGAGTGACGTGAGCATGACACAATGCATCCCGTGTTGATTCCAAACAATGCGGCGGTGTAAGCTTTTTGAAGCCCAT CATGGCCCACAACCAATTCAAAGACAGTCTTGATCCTCTCTCTTCTATAAATAGACACACAGAATCACAACACCAAAACA CATCCAAAATTCGTTTATTACCGAGAAAAGCAACAAAAAAAAAAAAGAGAGAAGAGAAGACGACTTTAAAGTATATGAGA GCTGCAATAAACAGAGCAAATTCACTTGGAGGTTTGTTTTCATTTAGGTTTATCAGAAACATCAAATCAATGTCTTCTTC TACTTCCCAAGATTTCGTATCAAGACCTGTTATCAAGAAAGTCTTCGCTAAGCTTCAGAAAGAAGGCGATGGAGCCGTCG TTAGACGTGGCATTTCCAGGAGTGAGCAGAAGTTGTTAGATCCTTTCTTGATGCTAGATGAATTCTCCGTTTCGCCTCCA GCTGGCTTCCCTGATCATCCTCACAGAGGGTTTGAAACTGTTACATACGTACTAGAGGGAGGAATCACTCACCAAGACTT CAAAGGTCACAAGGGTACAATATATGCTGGTGATGTTCAGTGGATGACTGCAGGAAGAGGAATCATTCATTCTGAAATGC CTGAAGAAGAAGTCAACAAAGGTTTACAACTTTGGATCAATCTTTCCTCCAACGAGAAAATGATAGAACCAAACTACCAA GAACTGTCGCATTCAGACATACCAAAAGCAGAACAAAACGGTGTTGAAGTCAAAGTCATTGCAGGAGAATCAATGGGAAT TCAATCACCTGTTTACACAAGAACACCTACTATGTTCCTCGATTTCACTCTCCAACCAGGAGCTCAAATCCACCAGAATG TTCCAGAATCTTGGAACGCATTTGCTTACATTCTAGAGAGCGGTGAAGGCGGCGGCGTTTTCAGTTCCTCGAACTCTTCG CCTATTCCAGCACACAGTGTTGTGGTGTTCGGACCAGGGAATGATGGTGTTAGCGTGTGGAACAAGTCATCTTCAAAGCA ATTGAGGTTTGTGTTGATTGCTGGAGAACCGATTGGTGAACCGGTGGTTCAATACGGTCCGTTCGTGATGAATACTCAAG CTGAGATTGATATGACCATTGAAGATTATCACTATGGTAAGAATGGCTTCGAAATGGCCAAGTATTGGAGGTCACAATGA GAAAGAACTAATAATCACCACTACTTTATTATTTCCGGTTCTTTTCTTTCTATTTTTAATTCTGTGTTCTAATTGGATAA ATAAAATGAAAATAAAAAT .........................................................................N...... .................N.......N........................N............................. ..........................................................................N..... .....................................................................i.......... ....................................................................N........... ..................................................N......N...................... ................................................................................ .......................N........N..........N................................i... ...........................................................i.................... ........................................................................i....... ...............................i.............................................i.. ................................................................................ ........................................N..N.................................... ...................................................................N............ ...........N.....................N........N..........N......................N... ................................................................................ .....N............. Pos Score Pred ------------------------ 74 0.492 - 98 0.249 - 106 0.198 - 131 0.362 - 162 0.374 - 315 0.184 - 390 0.810 Yes 469 0.312 - 531 0.461 - 538 0.276 - 664 0.170 - 673 0.165 - 684 0.457 - 717 0.796 Yes 780 0.711 Yes 873 0.710 Yes 912 0.781 Yes 958 0.675 Yes 1081 0.212 - 1084 0.186 - 1188 0.482 - 1212 0.453 - 1234 0.243 - 1243 0.161 - 1254 0.376 - 1277 0.227 - 1366 0.036 -

Explain the output -- Processing locus 649: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTATAGATTATTTACAGTTATCTCTATAGTATTATTTACATTTATATTTATTTTAAATTATGAAACTTAGATTAATTTG TTCTCTATATAAATAATTTAGACAATTATTCTTATGGGAGTCTTTTTTTTTTTTTCACTGAAATGTTGCCTTTCATTGAT ACCAAATTCTTATGGGAGTCTAACAGTAATTATTCATAAATACTTTTATCTTCGTACTCCCATAATTTATCGAACCATAT TGGGCCGTGGGAAAAGCCGATTAGTCATACAAAGCGTAAGAAAAAAAAAAGTTTCATTGTGTTGTGCCATTGTTTCACTT TCTCTCGCAATGGCGTCATTGAAGATGATAGACATAGCCGTTAACTTCACAGATGGGATGTTCAAAGGACTTTACCATGG CAAGAACTGTCATGTTCCGGATATTGCCACTGTCTTGAACCGGGCTTGGTCTGCTGGAGTCGATAGAATCATAGTAACTG GTGGGTCATTGGAAGAATCAAGAGAAGCTCTTGCAATTGCAGAGACTGATGGTAGGCTGTTTTGCACTGTTGGTGTTCAT CCAACTAGATGCAATGAGTTTGAAGAGAGTGGTGATCCGGAGAAGCATTACCAGGCTCTTTTTTCATTAGCCAAAGAAGG AATGCAGAAAGGGAAGGTGGTAGCAATTGGTGAATGTGGATTGGATTATGACAGGCTTCAATTTTGCTCAGTTGATATCC AGAAGAAGTATTTTGAGAAGCAATTTGAACTAGCATATGCTACAAAGTTGCCAATGTTCCTACACATGCGAGCAGCTGCT GAAGATTTCTGTGAAATTGTTGAGAGAAACAAGAATAGGTTCACTGGAGGAGTTGCTCATTCATTTACTGGTAGTGCGTC GGACCGCGATAAGCTTCTCTCTTTCGATAAAATGTACCTTGGTGTGAACGGGTGCTCGTTGAAAACAGCTGAGAATCTTG AGGTAATGAAAGGAATACCAGTAGAGAGAATGATGATCGAAACCGACTCTCCATATTGTGATATCAAGAACACACACGCC GGAATCAAGTTTGTAAAGTCGACTTGGCCTTCCAAGAAAAAAGAGAAGTATGATCAAGAGTCCCTTGTCAAAGGCCGTAA TGAGCCTTGTTTAGTCAGGCAAGTCCTTGAAGTGGTGGCTGGCTACAAAGGACTTGGCGATCTAAATCAAGTTAGCTCAA CCTTGTACCACAACACTTGCAGGGTCTTCTTCCCTCAGGACTTAGATTCTGCTGCAGATGCTCTGCTTTCCGGGCACCAC GAGACCGACTAGCATTAATCGTGACAGAAATTTGCAGTTTACTGGAATCAAGATATGTTGTTACATTACACCATTTATGG ATTAAAAACATTCTCAATAAAAATTACAAAC ............................................................N................... .................................N............................N................. ...........N.................................................................... ................................................................................ .........i..............i...........................N....i..................N... ...........N.................................................................... ................................................N............................... ........N....N.................................................................. ....................................N................i...........i.............. ................................................................................ ...............................N................................................ .....N.......................i..i............................................... .................................................N.............................N ................................................................................ .........................................................N...................... ......................................................N.....................N... ............................... Pos Score Pred ------------------------ 61 0.123 - 114 0.251 - 143 0.476 - 172 0.399 - 330 0.641 Yes 345 0.742 Yes 373 0.386 - 378 0.508 Yes 397 0.473 - 412 0.233 - 529 0.194 - 569 0.197 - 574 0.332 - 642 0.396 - 674 0.506 Yes 688 0.202 - 757 0.148 - 774 0.645 Yes 786 0.724 Yes 912 0.452 - 966 0.495 - 990 0.614 Yes 993 0.521 Yes 1090 0.485 - 1120 0.362 - 1258 0.201 - 1335 0.122 - 1357 0.052 -

Explain the output -- Processing locus 650: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGATTTCCTGGATGTTATTTAGAATTAATGAGTCCACCAAAGTGATAAGAATCCTAGTGACTATAAAATCATCTTTCTT TGCTATATAAACATTCTTTGAGTAAACAAATTAAACAAACCACACAAAACAAAACAAAAAAACATATTGATGGGGATGAA GAAGGTAAAGCTATCTTTGATAGCTAATGAAAGATCAAGGAAAACATCCTTCATGAAGAGGAAAAACGGGATATTCAAGA AACTCCACGAGTTGTCAACTCTATGTGGTGTCCAAGCTTGTGCTCTCATCTATAGTCCATTCATACCGGTTCCAGAGTCA TGGCCGTCAAGGGAAGGTGCTAAAAAGGTAGCTTCAAAGTTTCTGGAGATGCCGCGGACAGCCCGAACCAGGAAGATGAT GGATCAAGAAACCCATCTTATGGAGAGGATTACCAAAGCAAAAGAGCAACTAAAGAATTTGGCTGCTGAGAACCGAGAAT TACAGGTTAGACGATTTATGTTTGATTGTGTTGAAGGCAAAATGTCCCAGTATCGTTATGATGCAAAAGACCTTCAAGAT TTGCTATCTTGTATGAATCTATATCTCGATCAGCTTAACGGAAGGATCGAGTCCATTAAAGAAAACGGTGAGTCGTTGTT GTCTTCCGTCTCTCCTTTTCCTACTAGAATTGGTGTTGACGAAATTGGTGATGAGTCGTTTTCCGACTCTCCTATTCATT CTACAACTAGGGTTGTAGATACTCCTAATGCTACCAATCCTCATGTTCTTGCGGGCGATATGACTCCTTTTCTTGATGCG GACGCAAATGCGAATATGAATCAGGTTCAATACCAGGCTCCTAATAATCTGTTTAATCAGATTCAACGAGAATTCTACAA CATAAATTTGAATCTGAATTTGAATCTGAATTCAAATCAGTATCTGAATCAACAACAATCATTCATGAATCCGATGGTGG AACAACATATGAATCATGTTGGAGGGCGTGAAAGCATTCCTTTCGTGGACAGAAACTACTACAACTACAATCAACTACCA GCCGTTGATCTTGCTTCCACCAGTTACATGCCTTCAACCACCGATGTTTATGATCCTTACATCAACAACAATCTCTAATC ACAAAAGACGGAGATTTTCTAGTCTAAGTGTTCAGTTGTTTGAATTTGTGTTTTCTTCATTTCGAAAGACAAAAACTAAT GTTGTTTATTAATAATG ............N...............N................................................... .....................................................................i.....N.... ..........................N.........................N........................... ......................N........................................................i ................................................i..........................N..N. ...................i............................................................ .................N.......................i...............N..N................... ............N................................................................... ..................................................N............................. ...........................N..............N................i...............N.... .......N.......N................................................................ ................................................................N........N...... ........i......N................................................................ ...........................i...............N.....N.............................. ..............................................................................N. ..............N.. Pos Score Pred ------------------------ 13 0.162 - 29 0.256 - 150 0.653 Yes 156 0.246 - 187 0.240 - 213 0.417 - 263 0.283 - 320 0.689 Yes 369 0.779 Yes 396 0.362 - 399 0.234 - 420 0.538 Yes 498 0.236 - 522 0.670 Yes 538 0.089 - 541 0.142 - 573 0.273 - 691 0.125 - 748 0.109 - 763 0.066 - 780 0.673 Yes 796 0.086 - 808 0.076 - 816 0.320 - 945 0.331 - 954 0.311 - 969 0.546 Yes 976 0.468 - 1068 0.515 Yes 1084 0.154 - 1090 0.121 - 1199 0.060 - 1215 0.058 -

Explain the output -- Processing locus 651: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTCTCTGCTCTGGCTGGATCTGATAGTGATTTTACAGATTGTTCAGGTGCTTTATTGGGCTGTGTTTCTTCGTTAGTA AAGCTTTTTGCTTTCCTTTAGTTTAGTGAAATCTTAAAAATGTTTAACTTTGCGGCTTTACTTCAATTGCCTTATACACT GTGCAGGTCAGAAATGGGCAATCCGGGATCAGACACAGAGAGAAACAAGGGAAATGTGGTCTCAAGTGTGTTTGATCCTG CACTCTCTCGATTCGTTGGATTTTCCCAGAAACTCCAAACTCGTCTTAAGTCCCAGCTGAAGAATTTGACAGATGATAAG CATGGGATTGGTTCTTCATGGAGGCAAGATAAAGGAAACTCATCGATCTCCATGCAAATTGATCTGGAGAAACAGTTAGA TTGTTGGAGGGAAAACCCTTCGTGGACTGATCAGATCCCAGTCGTTAAGGTGGGTATTCCAAAAGGATCGCTTTGCAATC TTAAAGCCGAGGTGAATGTTGGTTTACCTCCTGATGCAGTCTATAACATTGTAATTGACCCTGACAATAGAAGAGTCTTC AAGAATATCAAGGAGGTTATGTCGCGGAAAGTATTGGTAGATGACGGGTTAAGGCAAGTGGTGGAAGTTGAACAAGCAGC TTTGTGGAGATTTCTCTGGTGGTCAGGAACAATCTCAGTTCATGTCTTGGTGGATCAGGATCGGGCAGATCACTCTATGA AATTCAAGCAAGTGAAAAGTGGATTCATGAAGAGATTCGAAGGAAGCTGGCAAGTAAAGCCTTTGTTTGTGGATGAACAT ATGTGCGATCGTTTGAAACCGAAAACGCTGGAGGAGTATAACAGGTGCACTGGAGGTAAAGGGAGGATCGGATCTAAGGT AACGCTGGACCAGCTAATACAGCCAGCGATTGTTCCACCTCCGCCCATTTCTTGGTATCTCAGAGGAATCACGGCTAAGA CGACAGAGATGCTTATCCATGACTTGTTGGCTGAGACCGCCAGGATCCGTGCAGCTGGAGTAATGGAGGATGGTCACTCG CCCGATGAGCAACGGATTGGCACACCTGGAGATATAAAAGAAAGATGGTCTGCACATAGAAGAACATCAAGGAGACGACG CAATGATTTGTGTTAAGTCACAGACTCACACAGTCCATGATTCTGGGATAATAGATATCTTGAGGATTTTTGGATCACCC AGCAATATGTGAACATAATATAATATTTGTTTTCA ................................................................................ .......................................i........................................ .............i.......................................i.......................... ........................................................................i....... ................................................................................ ...............N.................N.............................................. ..................N.....................N....................................... .........................................N..................................N... ..........................N.............................................N....... N............................................................................... ................................................................................ ........i.........N...........................................i......N.......... ....N.......................................N................................... ..i.................................N........................................... ......N............................ Pos Score Pred ------------------------ 120 0.827 Yes 174 0.626 Yes 214 0.520 Yes 313 0.563 Yes 322 0.487 - 338 0.442 - 372 0.220 - 496 0.154 - 514 0.194 - 579 0.220 - 601 0.300 - 682 0.457 - 717 0.313 - 747 0.223 - 793 0.301 - 801 0.481 - 969 0.782 Yes 979 0.460 - 1023 0.548 Yes 1030 0.470 - 1045 0.199 - 1085 0.167 - 1123 0.633 Yes 1157 0.043 - 1207 0.051 -

Explain the output -- Processing locus 652: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTACATTTACTAGATGGTAGGTTTGATTGGTAAAAACTAAGTTTTTTTAAACACTAGAAAAAAGAGAGAAAAAATAATGA GTGACAAAGAAAATTAAATAGAAATCATATATATATATATATAAATTCCAAAATATGATCAATGAGAGATCATGTGGTTG TCCCACATTATCTTTCTCAACGCACGCCTTACCATTAAGGAGACCCAAATTTCCTGCAACAAACAAAGTAAAAAAGTTGA GAATGGATTCATCGCAGGGAAGTACACAACAGAAGATTGTGATTCTGAACAGCCACAATGAGAACCTGGTGGGTCTGCTT CATGAAACTGGTTCTACAGAAATTGTAGTCTTGTGTCATGGCTTTCGATCAAACAAGAACTTCGAAATTATGAAAAATGT GGCTGTTGCTATAGAGAGAGAAGGGATTAGTGCTTTCCGTTTTGATTTCTCCGGGAATGGGTATGTTTTTCTTTCTTGCA TTATCTTGAATAAGTTGGTCTTTGTTTTGACTCTGGGGTTGTGGTGCTTTCATTCTTTCAGAGAGAGTGAAGGCAGTTTC TATTATGGTAACTACAATTACGAAGCTGATGATCTACATTCTGTTATCCAATACTTCTCTAACTTGAACCGTGTTGTTAC TATAATTCTCGGTCACAGTAAAGGAGGTGATGTGGTCCTCCTCTATGCCTCCAAGTATCACGATATCCCCAATGTGATCA ATCTCTCGGGACGTTATGATCTGAAGAAAGGCATAGGAGAACGTCTTGGTGAAGATTTTTTGGAAAGAATTAAGCAACAA GGATACATCGATGTTAAAGATGGTATTGTTATCGCTCTTCGCTTTTCATGCACCGTTATCAGAACTTTAGTGTTACTATC AATGTACTGGTTTGTTACAGGAGATTCTGGTTACCGTGTTACCGAGGAGAGTTTGATGGACCGGTTAAACACAGATATGC ATGAAGCTTGCCTCAAGATTGACAAAGAATGCAGGGTCTTGACGGTCCATGGATCGGGTGACGAGACAGTACCAGTGGAA GATGCAAAGGAGTTTGCAAAGATAATACCGAACCACGAGCTACAGATTGTGGAAGGAGCTGATCATTGTTATACCAATTA TCAAAGTCAATTGGTTTTAACGGTTATGGAGTTCATAAAGAGTCATTGTGAAGAAAAAAACGACAAGACTTGTAATTGAC CCTCCAATATTGCATTGGACATTTATTTATGTTTGGTTGACCAAATAAATTTATAAAAGGTAAATAAAAATATAATACTT ATATAATCATATGATAAT .............N..............................................................N... ......................................................i......N.........i........ ................................................................................ ..i......................................................N...................... ........................................................N.....N................. ................................................................................ ....N.......................N................................................... .............................i..............N..........................N........ ...............N................................................................ ..........N........N...........................N................................ N...........................i...................N............................... .........................N...................................................... ............................N................................................... ..........N....... Pos Score Pred ------------------------ 14 0.366 - 77 0.315 - 135 0.567 Yes 142 0.467 - 152 0.617 Yes 243 0.780 Yes 298 0.255 - 322 0.128 - 358 0.208 - 390 0.580 Yes 397 0.499 - 457 0.100 - 463 0.113 - 565 0.389 - 589 0.165 - 670 0.635 Yes 685 0.341 - 712 0.457 - 736 0.122 - 811 0.221 - 820 0.292 - 848 0.368 - 882 0.495 - 936 0.320 - 957 0.686 Yes 961 0.222 - 989 0.575 Yes 1009 0.092 - 1042 0.183 - 1146 0.126 - 1229 0.079 - 1291 0.042 -

Explain the output -- Processing locus 653: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTATTCACTACATTTACATAATTACTTCAAGTAACAATTTTACCACATTATTTCAATTAGTACTTAACCACCAATGCA TATTTGTTCCGAATTTCTTGATCGATAAATATCCAAATTAACAAGTTAATGAAGAAAAAAAACGAAGAAGATCAATGGTC AATATTTAATCAGAAGCATCCACGTCCACTTCATCAGATTCTATCTCTCTCTCTCTGTAAGATTCCTTGCTCTCGCGCTT TTCTCTCTCTCTCTCGCGTTCGTTCTTATCCACGAGCTCTGCACCGTCGCAATCTCCGTCTTCTCCATTTAGATCCAACA CAGAGCCTTTTCTACATGAAATTCGGCAAAGAGTTTCGTACTCACCTCGAAGAAACTTTACCAGAGTGGAGAGACAAGTT CCTTTGCTATAAACCTTTAAAAAAGCTTCTCAAATATTATCCTTATTACTCCGCCGATTTTGGACCCGCCAATTCCGATC ACAACGATTCGCGTCCAGTATTTGCTGATACTACTAACATCTCTTCCGCCGCCGACGACGGCGGTGTGGTTCCCGGCGTC AGGCCATCGGAAGATCTCCAGGGTTCGTTTGTGAGGATACTTAACGATGAACTTGAGAAGTTTAACGATTTTTACGTTGA TAAGGAAGAAGATTTCGTTATCAGATTACAGGAGCTCAAGGAAAGAATCGAGCAAGTTAAAGAAAAGAATGGGGAATTTG CATCAGAAAGTGAGTTCAGCGAAGAAATGATGGATATTCGGAGAGACCTTGTTACCATTCATGGCGAGATGGTGCTCCTG AAAAACTACAGCTCCCTTAATTTTGCAGGACTTGTCAAGATTTTGAAGAAGTACGATAAAAGAACAGGTGGACTTTTACG TTTGCCTTTCACACAGCTTGTTCTCCATCAACCCTTCTTTACTACAGAGCCACTTACTAGGTTAGTCCGTGAATGTGAGG CCAATCTTGAGCTTCTTTTTCCTTCAGAAGCGGAAGTTGTAGAGTCTTCTAGCGCAGTGCAAGCACACTCAAGCTCACAT CAGCACAACTCCCCAAGAATCTCAGCTGAGACTTCCTCAACTCTCGGCAATGAAAATCTTGATATATATAAGAGTACACT CGCTGCAATGAGAGCTATAAGAGGGTTACAAAAGGCTAGCTCGACGTACAACCCTTTATCATTCTCATCGCTTCTTCAGA ACGAGGATGATGAGACGGTAACAGCTGAAAACTCTCCAAACTCTGGGAACAAAGATGATTCAGAGAAGGAAGATACTGGA CCTTCCCACTGATCAGAAGAGAATGATGCTCTTTTTGATCAAGATTTTGAGAATTTGCTTCTTGATTTCACCCTAACTTT TCATAAAATTAACACATTTTACTTTACTTCT ...........................................................................N.... ................................................N.........................N..... ................................................................................ ................................................................................ ...............N................................................................ ................................................................................ ................................................................................ ..............................................N................................. ....................................................................N........... ..........................N..i..............................N.......N........... ................................................................................ ........................................................................i....... ................................................................................ .................................................N.............................. .......i........................................................................ ......N..N............................................N......................... ......................N..N...................................................... ............................... Pos Score Pred ------------------------ 76 0.414 - 129 0.345 - 155 0.373 - 336 0.249 - 607 0.257 - 709 0.257 - 747 0.318 - 750 0.641 Yes 781 0.122 - 789 0.104 - 953 0.678 Yes 1090 0.247 - 1128 0.655 Yes 1207 0.318 - 1210 0.434 - 1255 0.281 - 1303 0.061 - 1306 0.061 -

Explain the output -- Processing locus 654: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATACAGGTCATGCATGACATTACAAGACGTAACTGAATAAACTAATATCAACCAACTATCAAGAGTACATTTCTTTAAAT TTGTTTTAATTGATTTCTTAACTTATTATGTTAATTAATACAGTTTATTTCATACTCACTCTATTATATAAACTCATTTT GTCTCTCTTTCGTTGTCAACAACTTTTCTTCATTGACAAAAACACACAAATGGAAGGAAGAGTCAACGCTCTGTCAAACA TAAACGATCTCGAACTTCACAATTTCTTGGTCGATCCAAACTTCGATCAGTTCATAAACCTCATAAGAGGAGATCATCAA ACCATTGACGAAAACCCAGTTCTTGATTTCGATCTTGGTCCATTACAAAACAGCCCCTGTTTCATAGACGAGAACCAGTT CATCCCAACACCTGTCGATGACCTCTTCGACGAATTGCCTGACTTAGACTCCAACGTTGCTGAATCATTCCGTAGCTTCG ACGGTGATAGTGTTAGAGCCGGTGGTGAAGAAGATGAAGAAGATTACAACGACGGTGATGATTCTTCAGCCACTACTACG AATAATGATGGGACCCGTAAGACGAAGACTGATCGGTCTAGGACTTTGATCTCTGAGAGAAGAAGGAGAGGGCGTATGAA GGATAAGCTTTATGCATTGAGATCTCTTGTTCCCAATATTACTAAGATGGATAAAGCATCCATTGTTGGAGATGCAGTGT TGTATGTTCAAGAACTTCAGTCACAAGCGAAGAAACTCAAATCCGATATCGCGGGTCTTGAAGCTTCTTTAAACTCTACT GGAGGGTACCAAGAACATGCTCCTGATGCTCAAAAGACTCAACCTTTTCGCGGTATCAATCCTCCTGCTTCCAAAAAAAT CATTCAGATGGATGTTATACAAGTGGAGGAGAAAGGGTTTTATGTGAGATTGGTGTGTAACAAAGGAGAAGGTGTTGCTC CATCTCTTTACAAGTCTTTGGAGTCTCTTACAAGTTTCCAAGTGCAGAACTCTAACCTAAGCTCTCCTTCTCCGGACACA TACCTCTTAACATATACCTTAGATGGGACATGCTTCGAACAGAGCTTAAACTTGCCTAACCTGAAGCTGTGGATCACTGG ATCACTTTTAAATCAAGGTTTTGAATTCATCAAGTCATTTACTTGATTCTATAACGCTTGCTCTAACGTGAGTCAAATCC GGTTCTGCACTATATTGATTGTGTACCTTTCTTACATGTTTCATAACTTCCAGGGCTCTAATTTC .........N...N.................................................................. ...........................N.................................................... .................................................i.............................. ................................................................................ ................................................................................ .................i.............................................................. .................................N.......................N...................... ....N..N...................................................................N.... ...........N..................................N........................N........ ...N............................................................................ ................N........N...................................................... .......i...N.............................N...................................... ................................................................................ ......................N......N.................................................. ................................................................................ ...................................N............................. Pos Score Pred ------------------------ 10 0.159 - 14 0.298 - 108 0.221 - 210 0.881 Yes 418 0.661 Yes 514 0.192 - 538 0.241 - 565 0.232 - 568 0.243 - 636 0.260 - 652 0.193 - 687 0.394 - 712 0.285 - 724 0.203 - 817 0.249 - 826 0.070 - 888 0.593 Yes 892 0.286 - 922 0.161 - 1063 0.474 - 1070 0.399 - 1236 0.053 -

Explain the output -- Processing locus 655: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGTAGTGTACATGAGATGATAGTTGCCAAAACTACGTCATGCACTTCGAGACTCTATATTTCTAATGAAGTGTAACAAT CATGCAATTACCGTTAATTAGGAGCCTTCAAGCATGAGATCCTCACCTTCTCTCTCTTTCTCTCTCAGAGCTTTTGCATT GCATCACTCTATTTCCATAAAGCCTTTGTTGTTGTCCTTTCAGACATTTCTCAGCTTCTTCTTCTTCTTCATCTTCTCTC TTGTGTTTGAATACCAAAAAATGGGAAGACCTCCTTGTTGTGACAAGTCCAATGTCAAGAAAGGTCTCTGGACCGAGGAA GAAGACGCTAAGATCCTTGCTTATGTTGCTATCCATGGTGTAGGAAACTGGAGCTTGATCCCCAAAAAAGCAGGTCTGAA TCGATGTGGAAAGAGCTGTAGACTAAGATGGACTAATTACTTAAGACCTGACCTTAAACATGACAGCTTCTCTACCCAAG AAGAAGAGCTTATCATTGAGTGTCATAGAGCCATTGGCAGCAGGTGGTCTTCCATTGCACGAAAGCTTCCAGGAAGAACG GATAATGATGTGAAGAATCACTGGAACACAAAGCTGAAGAAGAAGCTGATGAAAATGGGGATAGACCCGGTGACTCATAA ACCGGTTTCTCAACTCCTTGCAGAATTCAGAAACATTAGCGGCCATGGAAATGCATCCTTCAAAACAGAACCATCTAACA ACTCTATACTCACACAATCCAACTCAGCTTGGGAAATGATGAGAAACACAACAACAAACCATGAGAGTTATTACACCAAC TCTCCAATGATGTTTACAAATTCCTCTGAGTACCAAACTACTCCATTTCATTTCTATAGCCATCCAAATCATCTGCTCAA TGGAACCACATCTTCATGCTCTTCCTCATCATCTTCTACTAGTATCACTCAGCCAAACCAAGTACCTCAAACACCGGTTA CTAACTTCTACTGGAGCGATTTCCTTCTCTCGGACCCGGTTCCTCAAGTAGTGGGATCCTCAGCTACTAGCGACCTCACT TTTACGCAGAACGAACATCATTTCAACATCGAAGCCGAATACATCTCTCAAAACATCGATTCAAAGGCCTCGGGAACATG TCATTCCGCGAGTTCCTTCGTTGACGAAATACTAGATAAAGACCAAGAGATGTTGTCACAGTTTCCTCAACTCTTGAATG ATTTCGATTATTAGATCCCTTCTTTTCCCTATTGTTGTTACTTCTCTTGTAACTGGTTTATGATTTTCAAGAACTATGTT TTTTCAATAAGTTATATCTCTTCTCTTATTAGT ...........N....i......................N.........................N.............. ................................................................................ ....................i..............................N............................ ......................N...........N............................................. ...N.......................N...............................i.................... ................................................................................ ....N..N........................................i.....i......................... ............................................N.....i............................. ...................................N..N.....................i................... ......N..N.....................................................................i ...............N................................................................ ................................................................................ .............................................................................i.. .................................................N...........................N.. ...........................................................N...............N.... ................................. Pos Score Pred ------------------------ 12 0.366 - 17 0.588 Yes 40 0.208 - 66 0.420 - 82 0.166 - 114 0.378 - 261 0.845 Yes 292 0.491 - 343 0.324 - 355 0.421 - 404 0.198 - 428 0.293 - 460 0.640 Yes 565 0.187 - 568 0.282 - 609 0.642 Yes 615 0.556 Yes 685 0.346 - 691 0.547 Yes 756 0.294 - 759 0.268 - 781 0.527 Yes 807 0.210 - 810 0.485 - 880 0.541 Yes 896 0.328 - 1118 0.641 Yes 1170 0.306 - 1198 0.229 - 1260 0.127 - 1276 0.094 -

Explain the output -- Processing locus 656: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCCTTATCTCCTTATATATAGATATATATAGATATATATTTTTTAACAACTCTTATCTCATTATATATATATATATATAT ATATTAGTTCCAAACCCTTAAACCCTGCAACTTTCTGTTTCTTCTCTAAATTGGTTTTTAAGAGATTATTTATTGGTTAT GATGTCTCAAACTAATAATCATGATACTAGGGTTACGAAGATTTTTGTGGGGAATTTAACATGGAGAACAACTGCAGATG ATTTGAGAAGATACTTTGAACAATTCGGACAAGTGGTTGATGCTAATGTTGTTTCTGAAACTTATCCTGGCAGATCAAAA GGCTATGGTTTTATTACTTTTAGAGACTATGTCTCTACAGTTAGAGCTCTTCAAAATTCTAAACCCATCATTGATGGAAG AACAACCAATTGCAATTTGGCTAGTGCTGGTGCAAAACAAAACATGAATCACCCTAACCTAAACGGATCATTCAATTATG TTATACCGCCGCATCAATATCAGCAAGCTCCTCAACACGTAATTCCATATTGTCCTCCGCACACCTGGAATCACGTTTGG GGACAATATATTTACATGTATAACAACAACCCATGTTATTATTACCCCACACAAATGATCCATAACCGCAGTGATTTTAT GACCCACCTTGCTTGGCATCAGACAAACAGACGACCTGTCATTATTTCTACGCCTCCAAGATCATCGGTTCAAACTGAAA GCATTAGCGAAACTGATCAAGAACTCATCGCGGATGTTGCACATCACGACAATGAAGAGGCGGTTACAAAATCAGATAGT GATGTTGATCAACAAAAGGCAAAGAATGTAAAAGGAAAGAACATTGGACAAGACGGTGACATCAAGCAAGATGCAAGGGA TCAAGAAGAAAAGATCAATGACCAAGAAGATAGTATCAAGCAAGATGTCGATGAAGAGCAAAACAAGATCAGTGGCATCG AGAAAGTTGTGGAGAATCAGGAAGTAAAGATCATGTGTGAACAAAAACATGGCACAAAACAAGATGAAGATGTAACAAAA GAGTTTGATTGTGGAATCGAGGTCACACACCAAGACAAGAGGGTCTTTGAAGAAGTAGATGAAGATACACCATAAGAAAA TGAAGTTGATCATGTAAACACTGGTGAGGAGAGACACGAAACAAACCACACTTATATTGCAATCAAACCTTTATTTTTTC TGTTAGCACGAAAC ................................................................................ ..............................................................................N. .......................................N.....N.................................. ....N.......................N............................................N...... ...........................................i.................................N.. ................................................................................ ...............i................N.....................i.......................i. ................................................................................ .................................N.................N............................ .................N..........................N.....N............................. ................................i...............N..............N.....N.......... ..........................................................N....................N ...........N.................................................................... .............. Pos Score Pred ------------------------ 159 0.453 - 162 0.456 - 181 0.151 - 221 0.395 - 238 0.308 - 280 0.103 - 286 0.188 - 325 0.109 - 349 0.280 - 394 0.309 - 444 0.745 Yes 478 0.289 - 576 0.566 Yes 593 0.475 - 615 0.545 Yes 639 0.714 Yes 754 0.356 - 772 0.231 - 802 0.176 - 826 0.515 Yes 871 0.241 - 898 0.143 - 925 0.244 - 931 0.216 - 993 0.753 Yes 1009 0.405 - 1024 0.225 - 1030 0.234 - 1099 0.108 - 1120 0.175 - 1132 0.056 -

Explain the output -- Processing locus 657: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAACAAACCAACTAACTCCTAACCCTATAAATCCTTCTCTTCCACGCTTCCTCATTTTCTCTCTCTCTGTTTCTTTTATT TTCTCGAGAAAATTCAGATAGAAAATCGCCATTGTTGTTCTTACTATCAGTCCCAGCGAGATCATGGAGATGGATCTATC AGAGGTTAAAGAAATGGAGAAAGACGAAGAAATTGATTCAGATCTCTCAGACTCAGATACAGAGGAGCCTGAAGAGAAGA TACTCGACGCCACTTCACTTTCAGCTCTTGATATGATACTCAAGTCTCTGTGGAACATTAGCGTTTTCGTAAGCAATCTT TCGATGGATTATGTTCAAGAAGCGAATCAGAGCCTCACAGATAATGAATCTGTTCCTCTACAAGACATTCTTCGTATTTT CATGCTCAACAATCTCAGTCTCACTCATCCTCTGGAAGCAAACGGTGTTTCTAAACTGTTTCTAAACATCTTGGAGCTTA TCCCTCGCTGGAACTATCACTTTGAAGTGAATGAAGTAGCGAAAACGATTTGCATTAGGTGCAAGACGGATATGGCATAT TCAGGTGAAACGTCTTATGGTATCATCATCAATGCTAATTCACTCAGAGTATACAAGTCTGCATTCAAGGAGTTTACATT TGAGAATATCATTAAGGCCAGCATGATCAATGTAAAGATGCTTTGTGACAAGGAAGGATGTGGGAAACGAAACTATGTTG ATACTATGATAAGTAACCTTCCATCTGCTTTTATAGTTGCACTTCAGTGGGAGAACAATGAGACCGAAAAAGAGATATTC GATACAGCTTCTGTTCTGGCGACTGAAATAGATATTAGTGCTATATATAGATATGTAGGTGACAGTGCATTTACCAAATA CCGTCTTGTCTCAATGGTTTGGTCGCATGGAAATCTATACAACTGTGTAGCTTATGAAAACAATAGATGGGTCAGACATT TTTGTTCTGAGATAGAGGTTATTGGAGACTGGGATGGTGTTCTCCGCATTTTTCGAGAACTGCATATTCGACCTGAGATT CTATTCTTTGAAAACGCTATTCTAAAGGACCAGATGTTTTCCGAGCAGGGATCTGAAGACTGAATAGAAGAAGCTTTTCA AATGGTGCAGGAAAGGAATTTGATGTAGAAGTCAAAAACTTTATAGGAGTAAGTATAGAACAATGCTTTTGATTGTTATG AT ................................................................................ ...............................................................i.....i.......... .............i.................................................................. ................................N............................................... ...N......N................................N.................................... ..............................N........................................N........ ................N..............N................................................ ......................N......N.......i...................N................N..... .....i...................................................N...................... ....................................................i........................... .............i............i..........................N............N............. .................................N.............................................. .................................N.............................................. .. Pos Score Pred ------------------------ 144 0.726 Yes 150 0.717 Yes 174 0.674 Yes 273 0.345 - 324 0.427 - 331 0.212 - 364 0.124 - 402 0.370 - 511 0.170 - 552 0.485 - 577 0.092 - 592 0.184 - 663 0.420 - 670 0.228 - 678 0.537 Yes 698 0.438 - 715 0.160 - 726 0.526 Yes 778 0.183 - 853 0.579 Yes 894 0.604 Yes 907 0.502 Yes 934 0.197 - 947 0.237 - 994 0.371 - 1074 0.397 - 1122 0.383 - 1143 0.045 - 1183 0.075 - 1198 0.035 -

Explain the output -- Processing locus 658: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCGTTTAAACTTGATTACCAATTGAACTCAGTAATTGCTTAGCTTGTTGATCAAACAAAATAAAATCCTTTTAAACACC TTTCCGAAAGTTAAAACCTAAAAGTTTCAATTCATGATTTTGAAAATAAGCGGCCGAAACATTATACACTTCCTCCACAC GCACAACAATAGACTCAAAGCAAACGCAACTCATATCCAATCGCTTTTAAACGCCAATAATCGCGTTACAGTAACAACTC GAAAAATGGTAACACTAATCGTGGCCACCACCGCCGATCCAGCGTCGATCAACCCTGCGGCGGCGCTTCTCGCCATGCCC GGTTGGACCGCCGGACCTATCTTACCGCCGGACATAAAGAGTTTTAGTAACAAGCAAACGAGAGTGATTCAACACGATAG ATCTATAGTAAAAGAAGACGATCTCGATTTACGTTGGGAAGAAGCTACCGGTGAAGTTGTTGATGAAGTCATCTTCCTCA GCCGTCACACCGCCGTCTCAAATCGTCCTGCTTTAACTGTTCATCCGATTGGAGTGCTTCATCTAAAGGATGGAGAATCA CCGCCGCAAGGAGGAAAGCCTGGATGGGCGGCGTTGCCAAGCACTAGAATTGGTCCATGGTTTCGTCTTTTGAAGAAAAT GGCTGAAGCTCATGGTTTAGTTCCTGAGTTTGAGATAACATTGGAAGCTACTCATCATGGACCAATAACTAACAAGCCAA CGATGTTTTTGGAGATTGGTAGTACCGAGGAATACTGGAAGAGGCAAGACGCAGCTCAAGTCATGGCTCTTTTAATGTGG GAAGGTCTTGGGCTAGGAGGCAGTGAAGAAGTAGGGAAGTGGAAAAGTGAAACCGGAAAGAGGAAAGTTCTTTTAGGCAT TGGAGGTGGACATTATGCTCCTCGTCACATGGATATAGCTTTGAAAGATGATATTTGGGTAGGCCATTTGCTTTCTGGAT ACTCATTACCGATGGAAGATCCAACTCAAACCAAAACAACGCCTGGAGAGAATTACATTGGTGGAAATTGGAGACAATCA ATTAAGGCAGCATTTGAAGCTACTAAAGCATCATTCCCCGGAGGCGAGATCTTGGCTCATCTTGACCACAAGAGCTTCAA AGGATGGCAAAAGAAGGCCATTACAGAGTTTTTAGCAGAAGAGAGCATCAATGTTGGGAAGCCAAACGATTTCACATGAA CTGTGAGAGACCCCCTTAAAACATATCTTGAGATCTGAGTTTTTGGTTTATTGGTTCAGAATCTATCATTGATGATGATG GGTTTAGATTATTTTCAG ................................................................................ .................................N.............................................. ................................................................................ .....i....................................................................i..... ................................................................................ ..............................................................N................. .....................................................................N.......... .......................N................................N.....................i. ...........N............................................N....................... ..i...........................................................N...........N..... ................................................................................ ..............N.............i..................N................................ ...........i.................................................................... ................................................................................ ...i..............................................N........................N.... .......................................................................N..N..N.. .................. Pos Score Pred ------------------------ 114 0.463 - 246 0.850 Yes 315 0.591 Yes 463 0.242 - 550 0.440 - 584 0.334 - 617 0.365 - 639 0.590 Yes 652 0.115 - 697 0.152 - 723 0.728 Yes 783 0.406 - 795 0.330 - 895 0.260 - 909 0.672 Yes 928 0.346 - 972 0.652 Yes 1124 0.582 Yes 1171 0.410 - 1196 0.315 - 1272 0.036 - 1275 0.065 - 1278 0.037 -

Explain the output -- Processing locus 659: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAAGCCCAACTTAAACTTGAATGTGTCTGAAGTCTAAACCAAATTACGTGTTAGATAATTGTGTTTGTGGTATTCTCT TGTCTTGACACATTTCTTTAGCTTCATTTAATGGCAGACAATAAAACATCTGAAGGTATTGTATCCGAAGAAACTCCCAT GGATGCTTCTCAGGAGCAACAACATGAAACAGTTGAGGAGATGCTTGCTCGCCACAGGCAAGAGATTAAACAACTTCAAA ACAAAGAGACAGAGATGAAGAAGGCAGCTGCCAAAGGTAGCAAAGCTGAACAAAAAGCTAAGAAGAAGCAAGTCGAAGAT GACATTTCCAAACTCTCCACTAAGCTCAAGGACAAACAGTTGAAAGAACTCGCCTCTCAAGGTTTCAGCAGCAGCAGCAG CAACAACATTGCCAAGGACGAAACGACTGAGAAAAAAGGAGACATCGACACTCTAGTTAGAGCTATAGCTGGAGTCTCAG TCACTGCTCAGCAAGAGCACTCAAAGCCTAGCAAGAGCGTGAAGAGGCGCGAGAAACGGGCTAAGGAAGAAGCAGACAGA GAGCAGAGAATTAAAGAGGAGCAAAGCCACGTGAAAAGTGATCGTATGGTTGAGAACGCCAAGCTTGAGAAGAAGCTTAA ACCATTGGGATTAACAGTCAGTGAGATTAAACCAGACGGACATTGCCTTTACCGTGCTGTTGAGAATCAGCTTGCCAACC GCTCGGGCGGTGCATCTCCTTACACATACCAGAATCTAAGAGAAATGGCTGCTTCGTACATGAGAGAGCACAAAACCGAC TTTCTCCCGTTTTTCTTGTCAGAGACTGAGGGTGACTCCAACAGTGGATCTGCAGAAGAGCGGTTTGAGAAATACTGCAG AGAAGTCGAGTCAACCGCTGCTTGGGGCAGTCAATTGGAGCTTGGAGCATTAACACATTGCTTGAGGAAACACATAAAGG TGTATTCGGGATCGTTTCCGGATGTTGAGATGGGCAAAGAGTATAGATCCGGCGATGACTCGAGCCTCATGTTATCGTAT CACAGGCATGCTTTTGGGCTCGGGGAGCATTATAACTCAGTGGTGCTCGTCAACAACATCACCGGATGATGGATGATGGA CTTTATCTAAAGCTTTAAGAAAAGTGCTTTTGTATCAAAAGTTTACTGAAATTCTTTCTTCTTTAATGTTTGTTGGATTT GAGAGAAC ......................N......................................................... ..............................i...............................................i. ..N....................N................i....................................... ..............i...............................................................i. ................................................................................ ................................................................................ ................................................................................ .............................................i.................................. ................................................................................ ............................................i..............i.................... ................................................................................ ................................................................................ .....................N.......i........................N.............N........... .......N.........................................................N..N...N..N.... .................................................................N.............. ........ Pos Score Pred ------------------------ 23 0.338 - 111 0.797 Yes 159 0.673 Yes 163 0.182 - 184 0.264 - 201 0.635 Yes 255 0.683 Yes 319 0.583 Yes 606 0.573 Yes 765 0.860 Yes 780 0.736 Yes 982 0.289 - 990 0.582 Yes 1015 0.228 - 1029 0.189 - 1048 0.235 - 1106 0.227 - 1109 0.263 - 1113 0.104 - 1116 0.169 - 1186 0.053 -

Explain the output -- Processing locus 660: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATTCATAATCAAATCAAACTTTGATGCATATTTGTTTGTTGTATTGGCCTACTTCTATAGTGTTGGTTCTTGACTGAT TTCCCCAATTGTTCGATTTAAGCTTTTTTGCTAATTGGATTGCTATGGAGGTCATTTGATGGGGTGGAAGTTCTGTGGTT TGATTTCGTTACTTCTTTTAATGGGATTCTTGTTGTTGTTTTATTTGTTACTCTAGGTAGTTTGTTCAATCAAGATGGTG GTACTTGTATGCATATGTCTAACATGGGTTTGGAAGAGATGATTTTTAATCAATTGGTACTGGTTTTGATGTTTCAACAG GTGGCAGAGGGTGTCCTGGATGAAAAATCGTTCAACGCTAGCATCTCGGACTACTCAAAACTCATCCATATCCATGCTAA AGAGAATCACATTGAAGATGTTGAGAGGATTCTAAAGAAAATGTCTCAAAATGGGATCTTTCCAGACATTTTAACCGCCA CTGCGTTGGTTCATATGTACAGTAAATCAGGCAACTTTGAGCGAGCAACAGAGGCATTTGAAAACTTGAAGAGCTATGGG TTACGGCCAGACGAGAAGATCTACGAAGCCATGATCCTGGGCTATGTAAACGCTGGCAAGCCTAAACTAGGTGAGAGGTT GATGAAAGAGATGCAAGCAAAGGAGTTGAAAGCCTCAGAAGAAGTTTACATGGCTCTGCTTCGAGCTTATGCACAAATGG GAGATGCCAATGGAGCTGCGGGGATTTCTTCTTCAATGCAGTATGCATCAGACGGTCCACTGAGCTTTGAAGCATATAGT TTATTTGTGGAAGCATATGGGAAAGCAGGTCAGGTGGATAAAGCGAAAAGTAACTTTGATGAAATGAGAAAGCTTGGCCA CAAACCCGATGACAAGTGCATTGCAAATTTGGTCCGAGCTTATAAGGGAGAGAACTCATTAGATAAGGCACTGAGACTTT TGTTGCAGCTGGAGAAAGATGGGATTGAAATTGGTGTGATCACTTACACCGTTCTTGTTGATTGGATGGCTAATCTTGGG CTTATCGAGGAAGCTGAGCAGCTTCTGGTTAAGATCTCTCAGTTAGGAGAAGCTCCACCATTTGAACTCCAGGTGAGCTT GTGCTGTATGTATTCGGGTGTGAGGAATGAGAAGAAGACTCTTCAAGCTCTTGGGGTTTTGGAAGCTAAGAGAGATCAAA TGGGACCTAACGAGTTTGACAAAGTTATAAGTGCGTTGAAAAGGGGTGGGTTTGAAAAAGATGCGAGAAGAATGTACAAG TATATGGAAGCTCGAAAGTTTTTACCTTCACAGAGATTGCAGATGGATATGGTTGCACCACCTCGAGCTTTTGGGTCTGG ATCTGGTAGAGTGAGACGATTTAACTCGTAAGTGGAAAAATCTTTTGAAACTTATTCACCATCGTGTGTTCAATGTTCTG AGCCAACTCACATTACGAGTTTCCATTGATTAAACTGTAGTTGTGTTCTG .........................N...................................................... ............................................N.............N..................... ....................N.....................................................N..... ........N.....N........i..............i.............................N........... ...................N.....................................................N...... .................N......................i.........N............................. ..............i............................................................N.... ..............................N............N.................................... ...N.....N.........................i......N..................................... ................N.........................................N....N................ ........N....................................................................... ..................N..............................................N.............. ................................................................................ .......N..................N....................................................i ............................................................N..........N........ ...i......................................N.....N............................... ........................................................................N....... .................................................. Pos Score Pred ------------------------ 26 0.297 - 125 0.169 - 139 0.316 - 181 0.112 - 235 0.355 - 249 0.257 - 255 0.303 - 264 0.760 Yes 279 0.658 Yes 309 0.289 - 340 0.176 - 394 0.137 - 418 0.368 - 441 0.676 Yes 451 0.453 - 495 0.600 Yes 556 0.488 - 591 0.280 - 604 0.175 - 642 0.612 Yes 651 0.533 Yes 690 0.373 - 709 0.223 - 717 0.669 Yes 724 0.306 - 730 0.300 - 756 0.630 Yes 763 0.209 - 817 0.419 - 859 0.265 - 864 0.398 - 889 0.274 - 979 0.215 - 1026 0.384 - 1128 0.499 - 1147 0.287 - 1200 0.592 Yes 1261 0.322 - 1272 0.311 - 1284 0.547 Yes 1323 0.402 - 1329 0.402 - 1433 0.063 -

Explain the output -- Processing locus 661: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTTTATAATCTAATGAATTATCTTAACACTTAAGTAACAACTAACAATGTACTATACTACTATGAATAACCTAGAAAAT AATTCTTTGTGGGTTAATTAATATTTTATAAAATGTTGGGGTAATTTAGAGAAAACATGGAAATATATACTATTTATTGA TAACTCCAAAACTATGCTATATTTCACTTTGGTGCCCCTCGTTTGTGAGAGTATGTTTGGTCCGTCGATGCACATGGAAG CAGCAGAGATAGTCCATTCGGCGGCGCGTTTTGAAGCTGAGAAAATGGGCAAGGAGGATGAGGCGGTGGAGGAGATACGA CGTATGAGTGGTTACGGCGGAGATGTGATAGTAGTAGGTGGGTTTCCGGCGTCGGAGTCGGAATCTGAATCTGAATCCGA TTTGGCGGCGGCGGAGATTATGGTTATATGGGCTATTCAAGGGCCAACTTCTTTTGCTCCAAACACACTCGTTGCTCAAT CTTCTCTTGAGCTGCGTCTCGACGCTTGTGGCCACTCACTCTCTATTCTCCAGTCGCCATGTTCATTGAACACGCCTGGA GTAACCGGGTCAGTGATGTGGGACAGTGGAGTGGTGCTAGGGAAGTTCTTAGAACATTCTGTTGACTCTAAGGTTCTTTC TCTTGAAGGCAAGAAAATCGTCGAGTTGGGTTCTGGCTGTGGCTTAGTTGGTTGTATAGCAGCACTTTTGGGAGGCAATG CTGTCCTCACTGATCTTCCAGATAGACTGAGGCTACTCAAAAAGAACATCCAAACCAATTTGCACCGTGGGAACACGCGT GGATCTGCTATTGTGCAGGAACTTGTTTGGGGAGATGACCCTGATCCAGATTTGATCGAACCATTCCCTGATTACGTATT AGGCTCAGATGTTATATACAGCGAAGAAGCTGTTCACCATTTGGTAAAAACGCTTTTGCAACTTTGCAGCGATCAAACTA CAATCTTCCTATCAGGAGAACTACGAAATGATGCTGTTCTTGAGTACTTCTTAGAAACCGCACTGAAAGATTTTGCGATC GGGCGTGTGGAACAAACACAATGGCATCCAGATTATCGCAGCCATCGAGTAGTGCTTTACGTTCTTGAAAAGAAGTCGAA GAGATGCCTCGCTGATGAATCTTCGCTTAATCAATCTTGTTAGCCATTGAGGTATTGTTTGTTTACACTTGACAACTGAA GTAGATGGCCCTAAGCGGGAAGTATAGTTCTGGCCTGATCCTTCCTTGCATATTTGCATACA .............N.................................N..............N................. ................................i.......................N....................... .............N......................................i..............N.....i...... ............................................i............N...................... ...i..................N......................................................... ...................i.......N.................................................... ..........................................................N..................... ...............i................................................................ .............................................................................N.. ................................................................................ ..................................N............................................. ........N....................................................................... ...........................i..i................................................. ....................N........................................................... ...N..........N................................................................. ....N......................................................... Pos Score Pred ------------------------ 14 0.323 - 48 0.314 - 63 0.359 - 113 0.556 Yes 137 0.445 - 174 0.499 - 213 0.749 Yes 228 0.370 - 234 0.783 Yes 285 0.868 Yes 298 0.371 - 324 0.526 Yes 343 0.105 - 420 0.600 Yes 428 0.486 - 539 0.396 - 576 0.616 Yes 718 0.142 - 835 0.147 - 889 0.292 - 988 0.696 Yes 991 0.638 Yes 1061 0.420 - 1124 0.453 - 1135 0.404 - 1205 0.052 -

Explain the output -- Processing locus 662: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCTGTACGACTTCTTCTCATTTTTCCATTCTTTAAGTCTTCCCGTTGATTCATTCCGATCTGAAATCTCTCTCTAGATT TCTCTATTTTTCGAATTTTAAATGAACGATTTGTTTTCCAGCTCATTCTCTCGCTTCCGCAGCGGAGAACCATCCCCTCG CCGAGACGTTGCCGGCGGTGGCGACGGAGTTCAGATGGCGAATCCCGCGGGATCAACCGGTGGTGTGAACCTCGACAAGT TCTTCGAAGATGTTGAATCTGTGAAAGAAGAGCTAAAGGAGCTAGATCGGCTCAACGAAACACTCTCTTCATGTCACGAG CAGAGCAAGACGCTTCACAATGCTAAAGCCGTTAAAGATCTCCGGTCTAAAATGGACGGTGACGTTGGAGTCGCGTTGAA GAAGGCGAAGATGATTAAAGTTAAACTCGAGGCGCTAGATCGTGCCAATGCTGCTAATCGGAGTCTCCCTGGCTGTGGAC CTGGTTCTTCCTCCGATCGAACCAGGACCTCTGTCCTCAATGGTCTCAGGAAGAAATTGATGGACTCTATGGATAGTTTC AACCGATTGAGGGAGCTTATCTCGTCCGAGTATAGAGAAACTGTACAGAGGAGGTACTTCACCGTCACCGGCGAGAATCC GGATGAACGAACCCTAGATCGACTGATTTCCACTGGAGAGAGTGAGAGATTCTTGCAGAAAGCAATACAAGAACAAGGAA GAGGAAGGGTGTTAGACACCATTAACGAGATTCAAGAAAGGCATGATGCGGTTAAAGACATTGAGAAGAATCTCAGGGAG CTTCACCAGGTGTTTCTAGACATGGCCGTGCTGGTAGAGCACCAGGGAGCTCAGCTTGATGACATCGAGAGTCATGTGGG TCGAGCTAGCTCCTTTATCAGAGGCGGAACTGACCAGCTACAAACCGCTCGGGTTTACCAGAAGAACACGCGAAAATGGA CATGTATTGCCATTATTATTCTCATCATCATCATAACTGTTGTGGTTCTTGCTGTTTTAAAACCGTGGAACAACAGCAGT GGCGGCGGCGGCGGTGGTGGTGGTGGGGGTACCACTGGAGGAAGTCAACCAAATTCAGGGACACCACCAAATCCTCCTCA GGCAAGGCGTCTATTGCGTTGAAGTTGAAGTTGAAGTTGAGTTTCGTTATTTGCATATATATTCTTTCTTTGAAAAACCT TATTATCAAACCAGCTTTGTGTTACTACTTTCTACTGCTGG ................................................................................ .....................i.......................................................... ..................................i............................................. .........N............................................................N......... ...................N...............................i............................ ..........i....................................N................................ .......................................N...................i........i........... ................................................................................ ..N............................................................................. ..........................................N..N.................................. .....................i....................................N..............N...... ...........................................................................i.... ................................................................................ ................................................................................ ......................................... Pos Score Pred ------------------------ 102 0.830 Yes 195 0.840 Yes 250 0.202 - 311 0.395 - 340 0.279 - 372 0.875 Yes 411 0.612 Yes 448 0.239 - 520 0.384 - 540 0.719 Yes 549 0.762 Yes 643 0.281 - 763 0.343 - 766 0.177 - 822 0.759 Yes 859 0.307 - 874 0.330 - 956 0.708 Yes 962 0.368 -

Explain the output -- Processing locus 663: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAAAAACTATTTAAGAAACATATGTTTTGGATCAAGTAAGGGTTTGATCTTGTACCGGAATCAGGTTTTTGCTTTTCTT CTAGGGTTTAAGGTTATTTAAAAACAGATGGAAACTTTCGGTGTGTTTCACAAGGAAGATGATGAGCAGATGGATTTGCC TCCTGGTTTTAGGTTTCATCCAACAGACGAAGAACTCATAACCCACTATCTCCACAAGAAGGTTCTTGACCTTGGTTTCT CGGCTAAAGCTATTGGTGAGGTTGATTTGAACAAAGCTGAGCCATGGGAGTTGCCATATAAAGCAAAAATTGGTGAGAAA GAATGGTACTTTTTCTGTGTGAGGGATAGAAAGTATCCGACTGGTCTGAGGACTAACCGAGCAACTCAAGCCGGTTATTG GAAGGCGACAGGGAAGGATAAAGAGATCTTCCGAGGCAAATCGCTTGTTGGTATGAAGAAAACACTTGTTTTCTACAGAG GAAGAGCTCCTAAAGGCCAGAAAACAAATTGGGTGATGCATGAGTATAGGCTAGATGGAAAACTCTCTGCTCACAACTTG CCTAAAACCGCTAAGAATGAATGGGTGATCTGCAGGGTATTTCATAAAACTGCTGGAGGTAAGAAGATCCCGATTTCGAC GTTAATCCGAATCGGTTCTTACGGAACCGGGTCTAGTTTACCACCTTTAACTGATTCTTCACCATACAACGACAAAACCA AGACCGAACCAGTTTACGTGCCCTGCTTCTCCAACCAAGCTGAAACTAGAGGAACAATACTCAATTGCTTCAGCAACCCT TCCCTTAGCTCCATCCAACCAGATTTTCTCCAGATGATTCCACTCTACCAACCTCAATCTCTCAACATTTCCGAGAGTTC CAATCCGGTTCTTACGCAAGAACAATCAGTTTTACAAGCGATGATGGAAAACAACAGAAGGCAGAACTTCAAAACATTGA GCATCTCGCAAGAAACCGGGGTTTCGAACACCGATAACTCATCGGTTTTTGAATTTGGGAGGAAGCGATTTGATCATCAA GAAGTTCCATCTCCCTCTTCCGGTCCGGTTGATCTTGAACCTTTCTGGAATTACTGAAGATTCAGAACCTGAAAAGTCTA TTAATTTAGGTCACAAGTAAGAAAATCTTTGCAAAGTTTTATCCTTTTAGCGTGGTGTGCTAAGCTTGTATAGATT ......................N......................................................... ...........................i..............................N..N.......i.......... ................................................................................ ...........................................i.................................... ..i............................................................................. ....................................................N........................... ...................................i...N..............N......................... ................N...i........................................................... ................................................................................ ................................................................................ .................................N.............................................. ........................................i..i.................................... ................................................................................ ................................................................................ ............................................................................ Pos Score Pred ------------------------ 23 0.482 - 108 0.579 Yes 139 0.154 - 142 0.260 - 150 0.717 Yes 284 0.647 Yes 323 0.673 Yes 453 0.316 - 516 0.619 Yes 520 0.224 - 535 0.257 - 577 0.378 - 581 0.714 Yes 834 0.414 - 921 0.520 Yes 924 0.687 Yes

Explain the output -- Processing locus 664: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAAGTCTCTTCTGAAGAAGAATTAGGGTTTTAGGGTTTAGAAAAAATTCTCTAAATATATTTGGTTTTAGATCTAATT TAAGATTCATTCACAAAATAGAAAACGTTTGTTTCTTCGTCATCTTCTTCTTCAACGTTGGATCTGTTAGTTATGTTTTT TCAGGGACCACATCTAAACTTGTCTCTAACAAAAAGTCCGGAACTTATCAACAAGATTGAATCTTACCTCAACGGACACT GCACATGTCCTCATCAACAAACAGAGAACTCATCAAAGAGATCAACTCTTCCTAAGAGCCCTGAGAAACTAAAAGCTATG AATTTCCCAATCTCCACGATCAGGATTGGCGGATGGGTCGTCGTTGCCAAAAACCCTGATGACATTGTGGCCAAATTCTA TTTTGCAAAGAAGAAACTTATATGGGAGTTTCTCTTCGGAGAACCAGAGACTAACACGCTGAGGCTGAAACGTAAGATCG AGATTCAATGGAATGATGTCTCATCTTTTGAAGAGAGTATTAGTTCACGTGATGAAACTGGAATCCTCAAAATCGAGTTG AAAAAACGTCCAACATTCTTCATAGAGACTAATCCACAGGCAGGAAAACACACTCAATGGAAACAATTGGATCACGATTT CACCGGAGATCATGCTTCTAATTACAGGAGACATACACTTCATTTTCCTCCCGGAGTTCTGCAAAAGAACTTGGAGAAGC TTGTAACCGACAGCTTCTGGTCCAAACTCTATGAAGTCCCTTTCCCGGTACATGAATCTCGTTACTTCGATAGTGGCTTT GAAAACAACTCATCTCGCAACAGCCACAGCCACACAGTTGGCTTTAACGTCAACAATGGCCCTCAACATCACTTTTCTCA AGGAATTGGTGGCGTGGGAGTGGGAGAAGGAAACTTTAGCATGGCTAACGGTGGATGGCAAAGAAACTCATATAGTCCAG TCAATAGTCTGAATTATAACACTGCTAATGAGTTCTTTGGAATGCAAGCTACCCCACCATCGTCTCAAGTGGTTAACGAG CATTATAACACGCAAAACGATTTCACCGGTTCACAGTTTGGTTATCACATGAACCAAGACGAGATCCAGAAGATACTTGA GAGTCAAGCATATGCAGTACCTGATACACAAACTAAATTGATGTCTTCAGCCATGAACAATGTCCCAAAGTATCCTCAGG CTGGATCGTGTCTAGAAAGCATGTTGTTCTGGTATGAACATAGAAGAAGATTTCAGCAGTAGACATAATTAGGGTTTTGT TGAGATATGGATCAATGATGATGGTTCTCTTGAGTCTTGTAAATAAAGTGCAGAAATCGCAACTTTAATTACAAGTCTGT ................................................................................ ........................................................................N....... ................................................................................ ....i........................................................................i.. ................................N.........................N..................... .....................i.......................................................... .......N....N..N...................................N............................ ........................................................i....................... ...........N.................................................................... ..............................N....................N............................ .......................................................i........................ ........................................i.............N......................... ...........................N.............i...................................... ................................................N............................... ...........N............................N...........N......i.................... ....................N............i.............................................. ......N.......N..N..N........................................................... Pos Score Pred ------------------------ 153 0.380 - 245 0.565 Yes 318 0.712 Yes 353 0.447 - 379 0.308 - 422 0.550 Yes 488 0.178 - 493 0.231 - 496 0.365 - 532 0.135 - 617 0.569 Yes 652 0.246 - 751 0.422 - 772 0.330 - 856 0.696 Yes 921 0.801 Yes 935 0.334 - 988 0.270 - 1002 0.773 Yes 1089 0.363 - 1132 0.245 - 1161 0.337 - 1173 0.321 - 1180 0.506 Yes 1221 0.099 - 1234 0.512 Yes 1287 0.102 - 1295 0.084 - 1298 0.072 - 1301 0.057 -

Explain the output -- Processing locus 665: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAACTGAACAAACGTGTCAGCTATCTTCTCTCTCTCACTATTTCTGGGTTTGTATCTTTCTTCTCCCCACTCCGATCCT TCATTAATCTCGATGATCTAAAATATCCCTTTCTTAACATTTATTATCTTCTTGGAAAAGCCATCAATGTCTTCATCATC TATGCCATTATTCCCATTTTCCCTTTATAATTTTATATACGAATCACTAACTAAAACAAGTATATCTTTCAGGTTGAAGA AGATGAAGAACAAAGCATCCATGGAAGCTCCACAGTTGCCATCTCTCTCTGACCTTGAAGCTCGGATGCAAATCCTGCGT GAGTCTGGTTTTCAAGAAAGCCAAGGAGATGATGATACCTTTGCTCAACGAGCTGAATGGTTTTACCAGGGACGACCTTT GCTTCTCTCACTTTGTCTGGACCTGTACAACGGCTACGTCACTCTCTTGGGTCGTTCTTCTCATCAGACCAGACTTAAAC CCACTACTTCTCTTCCGAACCAGCTTCTCCAAGATGATGATGACTGCATTTCGGACATTGATTCCGTTAGTGAGGTCAGT TCTGAAGTTGAGAGCACCTTATCCTTTCAACAGATGAAAGATCCTACTGCGGTTTCTGAGAAAGTCGATGAGCTTGTATC CCAACTTGTGACAGCAAGCTTGGACAAAGAGATTCTGAAACACGAGTTACTTCACAAGGACCAACAGTTTCATGAAGCCT CCAAGACTATAGAGCTGTTGAAGAAGTTTGTCATGTTGCTGGAGATGGAGAAAGAAGTGGCTGTGGAGGAAAACGCTAAT CTTGGCTACAAACTCACTTCTCTCTTGGAGGAGAACAGAGAGCTAGCCACTGAGGCATTGTTCATGAAGAACGAAGCTGT TGGGCTTGCTAGGTGTGTGCTTAAGATGAGAGATGACCACTTTCACAAGGTCTGCATTCTCCAGAACCGCATCTACTCGC TTCAGGCATCTAGGAACTCAGAGCCCGTCTCTGATAAGGTCTCATACGGATGCTTTGGCCTGGATAAGCATAAGACCAAA AAGAAGAAGGAGAACAAAACCGAAGAGAAGAAGCCTGGATTCAAGTGGTTGAAGAAACTGAACACCATTAACCTGTTTAC AAAGTGCAGCCTTAACCCATCGGCTGCTGCTCCATCATGCTGCACTTTCGACTTGCCTTATTAGCTTAATCCTTTGCTGT ATTAGTAAACTATGAACAATGCATACATAACTGTGTATGTTCTATCGCCTTATTAGGTTTAATGCTTTTCAGTGCATCGC CCT ................................................................................ ............N.....................................................N............. ..i.................i............................................N.............. ............................N..N........................N....................... ................................................................................ .................................N..N..i........................................ .................................i.................................N............ .......................................................................N........ ................................i...........i................................... ...............................................................i................ .........................N......N............................................... .................................................i.............................. ................................................................................ ....................................N........................................... ...........N......N.................N........................N.................. ... Pos Score Pred ------------------------ 93 0.093 - 147 0.456 - 162 0.373 - 243 0.642 Yes 261 0.535 Yes 306 0.304 - 349 0.337 - 352 0.418 - 377 0.326 - 514 0.137 - 517 0.134 - 520 0.549 Yes 594 0.571 Yes 628 0.206 - 712 0.116 - 753 0.703 Yes 765 0.594 Yes 864 0.602 Yes 906 0.414 - 913 0.442 - 1010 0.673 Yes 1157 0.204 - 1212 0.104 - 1219 0.196 - 1237 0.067 - 1262 0.045 -

Explain the output -- Processing locus 666: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATTAAATTTACAACATGGCAAGGAATGTTACAAAGTCGCTCGCACGCCTTTGCAAACGTTTCTAAGCTTAATTATCTA AACAAATAATATAACCTTTGACTTTCGCATGTATTCGGCATGTATATGAGGTGTATTAATCTTTTAGGCTCTAAATTATA TTTCGAAGGTTGGCTTTATATATATATATATACCTTATGGTTGATCTTAAATGACTCACAACCCTTTCCACAAGAAGTTA TAAACCAATACATAGAAGAAAACTTCCAACCCCAAAAAATGATGAAGGGTGCCAAGTTTTCATCTCTTCTTGTGCTTTTC TTTATTTTTCCGATCGCATTTGCTCAACTGAGAGTCGGGTTTTATAGTCAATCATGCCCTCAAGCCGAGACTATCGTACG CAATCTGGTGCGCCAACGGTTTGGTGTTACCCCAACCGTTACCGCCGCTTTGCTCCGTATGCATTTCCACGACTGTTTCG TTAAGGGCTGTGACGCTTCTCTCCTCATTGATTCAACCAATTCCGAGAAAACTGCTGGACCAAACGGAAGCGTCAGGGAA TTTGACCTGATAGACCGGATCAAGGCTCAGCTAGAAGCTGCATGCCCTTCCACAGTCTCATGTGCTGACATCGTCACATT GGCCACACGTGACTCGGTGGCCTTAGCCGGAGGCCCAAGCTACAGCATCCCCACGGGAAGGCGTGACGGTAGGGTCTCAA ACAATCTTGATGTAACCTTACCGGGTCCAACGATCTCCGTCTCTGGAGCCGTGAGTTTATTCACGAACAAAGGGATGAAC ACGTTCGATGCAGTAGCTCTTTTGGGTGCACACACTGTTGGTCAAGGAAATTGTGGTCTCTTTAGTGACAGAATCACTAG CTTCCAAGGAACTGGACGACCGGACCCGTCCATGGACCCCGCTTTGGTTACCAGCCTAAGGAACACATGCAGAAATAGCG CGACGGCGGCACTAGACCAGTCGAGTCCATTGAGATTCGACAACCAGTTCTTCAAGCAAATCCGTAAAAGGAGAGGAGTG TTGCAAGTTGACCAACGCCTCGCATCCGACCCACAGACTCGTGGGATTGTGGCTCGGTATGCTAATAACAACGCCTTCTT CAAGCGTCAGTTCGTTAGAGCAATGGTGAAGATGGGAGCAGTTGATGTGCTTACTGGTCGTAACGGTGAGATCAGAAGGA ACTGCAGGAGATTCAACTAATGAACTACAGAAGCGTGGATATAATATTACATTTGATACAATATATTTCAAATCTTCTTT ATATTTTTACTAATTCATTTTTGGTTTTGGTTGGGGTTT ................i.........N..................................................... ............................N..........N.....N.................................. ....................................N.............N............................. ......................................i..i...................................... .....................................................i.......................... ..........................................................i..................... ................................................................................ .........................................N.................i.................... ................................................................................ .........N................................................................i..... .......i........................................................................ ...............................N..................................i............. ................................................................................ ..........................................................N..................... ......................N........N............N................................... ...................N............................................................ ....................................... Pos Score Pred ------------------------ 17 0.584 Yes 27 0.292 - 109 0.191 - 120 0.166 - 126 0.204 - 197 0.338 - 211 0.489 - 279 0.811 Yes 282 0.679 Yes 374 0.668 Yes 459 0.585 Yes 602 0.355 - 620 0.567 Yes 730 0.302 - 795 0.786 Yes 808 0.751 Yes 912 0.417 - 947 0.655 Yes 1099 0.181 - 1143 0.456 - 1152 0.461 - 1165 0.477 - 1220 0.134 -

Explain the output -- Processing locus 667: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCTTCACACCATTATTCAACATGTTATGACTATGACTCTTAGAGAGTTTCTATTATTAGGTCTGATGACTGATGCTTTTG GTAATAAAACACATTTGGTGAAGTTTTCATTTATGTCATATATCATTGAAGCTTTCGCTAATATACTTGTGGAAAATGGA CTCACAGGTTCTCTTCTACATTGCTGCTGAAGATGAAGCCTTCTTGGCTGTTAACCGAGAGAGGGATGCTCAGTCACTCA TGAAGGAGAGGGAAAGTGTACAAGATCAGTCTGATCCGAGTGAGACAGACTCTGAGGAGCTATTGCAGTTAGATGGAAAG AGTATATCTGAGTGGGTGAGTGAAATAGATGCAATTTCCAAAGAAGTAGAGGCAGAGTTAGTCTCACGAGATATCGGCTG TCACTTGGTTCAAGTTCTTGAAGCTGTAAATACAGTTCTCTTCGATCTGAGAGGCTTCAAGAGGACATCTATTACTTTAG ACCCGGAGAATTCCTACCTTCACTCTGTACTTAACTGTAGATGCAGCACTGCGTTTCTGATTAGTGTAATATACATTGAA GTTTGTAAGCGCCTGAATGTACCAATTGTTGGATCTCCAGTTGGGGAAGATTTTCTGATTTGGCCTAAAACGGAGTATCC TGAGGAACTCTTTAAAGCAACTTCAGGACAGAGCTTGTTCTCTATTGTCAATGGAAGGTGTGTTGATGATCCTGGATCAA TGGCATCAGACTTAACTGCAAAATCACTTCAAGACCTCGACATGGCAACAAACAGAGACATTATAGGGATTGCTCTAGCC AATTTGATTAGGCTTCACTGGAGACGTGCTTCTAAGTCATCCCATGGACTTATGCTGACTTCTCCTCTTAGTCAACTTAA CAACATCAGTAGTTCTAATTTCCCATTGTTGCGGCCTCAAGATCTTAGGTTGGCTATTGCGGCTGCAGAAAGGTTGTTGA TTTTGCAACCTCATAACTGGGCACTTCGCAGAGACCTTGGTATGATGCTGTACTACGACAGGCAATATGGAGAGGCGGTA CAAGAGCTGAGCATATGTATGGCATTTGCTCCTCCTGAAGAAGAAGCTGTGTTGGAGCCATTTGTAGAAAGACTTCATCT CCTTCGTCTCATATCTTCATTGAAGCCTCTTGGTTCTGATCGCTTGACCGTTCCTTGACTTCTCTCTCTCTCTCTCTCTA CTCCACTTGTAAATTCACTCTCTTTCTTTCTCTGTTGTGTGTTTGAAGAACCTTTGTAGTAGGAGACATGGAGCTTT ....................N....N.....N................................i......N........ ................................N..........................................i.... ................................N................................N.............N ........................................................................N....... ............................N................................................... ................................................................................ ........................................N....................................... ................N............................................................... ..................................................N..............N.............i .........................................i...................................... ...........................................N.......N............................ ................................................................................ .........................................N..N.....................N............. ..............N...N............................................................. ................................................................................ ...................................................................N......... Pos Score Pred ------------------------ 21 0.192 - 26 0.296 - 32 0.300 - 65 0.645 Yes 72 0.177 - 113 0.224 - 156 0.735 Yes 193 0.229 - 226 0.135 - 240 0.487 - 313 0.111 - 349 0.095 - 521 0.144 - 577 0.157 - 691 0.415 - 706 0.113 - 720 0.698 Yes 762 0.632 Yes 844 0.191 - 852 0.461 - 1002 0.306 - 1005 0.351 - 1027 0.259 - 1055 0.236 - 1059 0.299 - 1268 0.083 -

Explain the output -- Processing locus 668: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGGCTGACGGAAGCAATGACATCATCACATACTCTGTATGTCGATCATCCAATTAGTCCAAGCCACGTGTAAACCTCAGC TGGCTGCGCGGTCAACGTTGAAAAAGAAAAAGGTTGGTTTTTATCACACGTGTCCGAAAGAAAACTACACACGTGTGGTT CATGAACCTCCCCTTCGTTTATAAAAGCTCCATGGACTTTGCTCTCTTCTCCTCGATTTCAATCTTTGAGATAAACCACA AAGATCCTCCGATTCGAAGGTTTATAAAAACTCAAAATCGAATCTTATCCACAAGAAAACAACAAGGTACTTTTCCAAAA ATGAAGGCGGAGTTGAATTTGCCGGCGGGATTCCGATTTCATCCGACGGACGAAGAGCTTGTCAAGTTCTATCTTTGCCG GAGATGTGCGTCAGAACCGATTAACGTTCCGGTTATCGCAGAGATTGACTTGTACAAATTCAATCCATGGGAGCTTCCAG AAATGGCGTTGTACGGTGAGAAAGAATGGTACTTCTTCTCGCATAGAGACCGGAAATACCCAAACGGGTCGAGACCAAAC CGGGCAGCTGGAACCGGTTATTGGAAAGCGACTGGAGCTGATAAACCGATCGGAAAACCGAAGACGTTAGGGATTAAGAA AGCACTCGTCTTCTACGCAGGAAAAGCTCCGAAAGGGATTAAAACGAATTGGATTATGCACGAGTATCGTCTCGCTAATG TCGATCGATCTGCTTCTACCAACAAGAAGAACAACTTAAGACTTGATGATTGGGTTTTGTGTCGGATATACAATAAGAAA GGAACAATGGAGAAGTATTTACCGGCGGCGGCTGAGAAACCGACGGAAAAGATGAGTACGTCGGACTCAAGATGCTCAAG TCACGTGATTTCACCGGACGTCACGTGTTCTGATAACTGGGAGGTTGAGAGTGAGCCCAAATGGATTAATCTGGAAGACG CGTTAGAGGCATTTAATGATGACACGTCCATGTTTAGTTCCATTGGTTTGTTGCAAAATGACGCCTTTGTTCCTCAGTTT CAGTACCAGTCCTCCGATTTCGTCGATTCGTTTCAGGACCCGTTCGAGCAGAAACCGTTCTTGAATTGGAATTTTGCTCC TCAAGGGTAAAAATAATCGGCAAAAAGTTGAAGCTTTTCAGAGTCTTCGATCACCGGCATTGTGTCGGATCCTGACCCGG AGACCAAGTCGGGTCATACGATTACATAA ...............i.....................N.......................................... ................................................................................ ................................................................................ i............................................................................... ...N..............................................................i............. ..N......................N...................................................... ................................................................................ .......................................................N.....................N.. .............................................N.................................. ......i............................................i...................N........ ............................................................i................... ...............N..N..........i...........................N...................... ................................................................................ ................................................................................ ............................. Pos Score Pred ------------------------ 16 0.793 Yes 38 0.442 - 162 0.137 - 192 0.393 - 321 0.646 Yes 404 0.360 - 467 0.821 Yes 483 0.483 - 506 0.421 - 696 0.325 - 718 0.179 - 766 0.217 - 807 0.829 Yes 852 0.712 Yes 872 0.423 - 941 0.538 Yes 976 0.163 - 979 0.341 - 990 0.520 Yes 1018 0.213 -

Explain the output -- Processing locus 669: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAACATTAATTAAATCTAACTTCAATTGATTTTTCTTTTCTGTTTTACGATTTCATTTTGATTTATTGTTTATGCTTTTA CAATAGTAGATATGAATTTAAATTTTTATATATAAATTATGCAAAGACACATTATATTTCCTCATGTGCTTTCTAGAAAG TGTTAGCCAACTTTTCATTAATAGTTATGCATCTAACAAAGGATGCATGAATTTTGTGAAGATGAACAAGGGTGGAGCTC AACAGAAAGGTCATTCGTCAAAGCAAGCTGAAAACATAGTTTTAGTAGGCCGGACAGGGAATGGAAAAAGTGCGACAGGT AACAGCCTCATTGGAAAAAAGGTGTTTGCTTCAAAGGCACATGCTTCTGGTGTGACCATGAAATGTCAGACACATGGAGT TGTGACAAAAGATGGTCACAAAATCAATGTGATTGATACTCCAGGTCTGTTTGACTTGTCAGTGTCGGCTGAGTATATAA GTAAAGAGATTGTTAGATGTCTAACTCTAGCAGAAGGAGGGATACATGCTGTGCTCTTAGTTTTATCTGCAAGGACTCGA ATTACTCAAGAGGAAGAGAACACACTTAGAACCTTACAGGCTCTATTTGGGAGTCAAATTCTTGATTATGTCGTCGTTGT TTTCACCGGTGGTGACGTGCTAGAAGAGTGCAAGGAGACACTAGAAGATTATTTGGGTAGAGATTGCCCTACTTTCATAA AGGAAGTGATGAGGATGTCTAGTAACCGAAAGGTTGTGATCGATAACAAGACTCATGATGAAGGCAAAAAAGCAGAGCAA GTCCATAAGCTTCTCTCCCTAGTAGATGATATCAGAAGGAGCAAGTGTGGAGAAGCATATACCGATGATACATATCATAT GATAAAGGAAGAGTCAGAGAAACTAAGGAAGCATCATGAGGAGCTGGAATCAAAGAACTATTCAGAAGAATGTGCAGCTG AAATGAAGAATCAGTCACTTATATTGTATAAGGAGAATTTGAAACAAATGTCAGAGCAGTTGGAGAAGAAGCTCAAAGAT GCTGCGGAGGCGCAAGAAAAAGCGCTAAGCAAGATGACACAAGAAAACAACGAGCTGAACCTGGCGCTTAAGATTCACAT CCCGTTGCCGCCAATTACTCCATGCAACATTCTTTGAAAAATGTCTTCTTTCTAACAAAGGGGTTTCTCAAGAAATATCA GTAACTATTATCGTCCAAAGGGATTTTGGGTTGCCATAAGAAGATACAATCAAGGA .......................................................................N........ ...........N..........................N........................i................ ..........................N...............N...i..............i.................. ............................................................N................... ........................................N................N....N..........N...... ...........N..............N..................................................... ................i............................N.................................. ...................................................................N............ ................................................................................ ........i.....i.......................................N..N...................... .........................N......................................N.............N. ...................................N.................................i.......... ..i............................................i..............................N. .................................N.............................................. .....................N..................N....................................... ........................................................ Pos Score Pred ------------------------ 72 0.153 - 92 0.310 - 119 0.268 - 144 0.544 Yes 187 0.253 - 203 0.304 - 207 0.611 Yes 222 0.767 Yes 301 0.220 - 361 0.103 - 378 0.489 - 383 0.431 - 394 0.093 - 412 0.359 - 427 0.213 - 497 0.601 Yes 526 0.183 - 628 0.192 - 729 0.661 Yes 735 0.691 Yes 775 0.297 - 778 0.252 - 826 0.193 - 865 0.149 - 879 0.383 - 916 0.162 - 950 0.722 Yes 963 0.723 Yes 1008 0.707 Yes 1039 0.155 - 1074 0.329 - 1142 0.359 - 1161 0.188 -

Explain the output -- Processing locus 670: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGAAAAATAATAACATAAAATAAAATTGTGAAAAATAATAACATAAAAATGAAGAAATGATTTTATTACTAAATAAATT TGGTAACAAATAAAGTTTTAGTTTCCGTTTACTCCAATGTTACTATTTTTTTTCCCCCGACCATTTTTACACACAGCCAA TCAGAGCCTTGTTTCATCGTCTCTTGTCGAGGCGATAATATTTAGATCTGCTCACCATTTCTCCGCCATCGCCGACGGAA GAAGAAAGATGGGAGACAACAACCCTAACCGATCAGAAGCAGAACGTCTTCTCGGAATCGCGGAGAAGCTTCTCGAGTCA CGAGATCTAAACGGTTCAAAAGAGTTTGCAATCTTAGCTCAAGAGACAGAGCCACTCCTCGAAGGCACCGATCAAATCCT CGCCGTCGTCGATGTCTTACTCTCATCAGCACCAGAGAATCGTATCAAAAACCAACCAAACTGGTACAAAATCCTTCAGA TCGAAGATCTAACTGAATCATCAACAGACAACGATCTAATCAAGAAACAATACCGTCGTCTTGCTCTTCTTCTCCACCCT GACAAAAACCGTTTCCCTTTCGCCGATCAAGCTTTCAGATTCGTGCTTGATGCATGGGAAGTTCTATCAACACCTACGAA GAAATCTCAATTCGATGGAGATTTGAATCTCATCTTCACTAAAGTAAATCTCAACACTCAGAAATCGAAGAAGAAAACAA CAACGAATGAGAAGATGTCTACGTTTTGGACGGCGTGTCCGTACTGTTACAGTCTTCATGAGTATCCTAGGGTTTATCAA GAGTATTGTATTAGATGTCAAAACTGTCAAAGAGCGTTTCACGCTGCGAGTATTCCTCAGTTGCCTCCGTTGATACCTGG TAAAGATGAGTATTATTGTTGTTGGGGTTTTTTTCCGATGGGGTTTGTTGGTGGTAAAGGAGGAGAAGCTGCCATTGCTA ATGGAGTAGATGCAGCTAAGTTCCCTAATTGGATGCCTCCGGTTTTCTCATCCGGCGGCGTTGCAGCTCCTCCAAGTGGT AATGGTGTTAGTTTTGATGGATGGTCAGGTGGTGCGGCGAAGAGAGATAATGAGGCTGTGAGGAGTAATAATGGTGTTGG AGTTAATTCAGATGGAACACCGAAGAAGAGAGGAAGAGGAAGGCCGAAGAAGAATCCGGTTTAGTGATCTCTTTTCAAAC TCCGGTCATGATTGTTAAAATTATGGAGACAAAATTTTAAAGGCAAGTTTTTTTTAAGGCAAGGAAGTTTAGGATCAGCG TTT .................................................N.......i...................... ....................................N........................................... ................................................................................ ........i....................................................................... ................................................................................ ...........N.................................................................... ................................................................................ .................................................N...i.......................... ..............N................................................................. ......N.......i..........................................N...................... ..............N................................................................. .....i...............................i.......................................... N........N......................i............................................... ...........N.................................................................... .......N..............N......................................................... ... Pos Score Pred ------------------------ 50 0.402 - 58 0.685 Yes 117 0.179 - 249 0.610 Yes 412 0.240 - 610 0.146 - 614 0.659 Yes 655 0.154 - 727 0.292 - 735 0.598 Yes 778 0.361 - 815 0.249 - 886 0.568 Yes 918 0.651 Yes 961 0.267 - 970 0.122 - 993 0.730 Yes 1042 0.175 - 1057 0.320 - 1061 0.279 - 1090 0.318 - 1111 0.349 - 1132 0.417 - 1208 0.075 - 1223 0.046 -

Explain the output -- Processing locus 671: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACAAAGGATTATAAAATTGAAGAAATCATGACTTCAGTGATCGAATTCTTTTAGTGATTAAAAAAATCAAGAAATTATG AAAATAACACGAAATAATTAATTTGCAAGGATGATAAAAAAAATGGTAAATTTGGTAAACGTATTTAACATGTCAAAGTC AAACCCTTCAAATCTCAAATATTACAATCTCCATCCCTCTTTTAAACACCATAAAGCCTTAAACCTCAAAAGACATTTCA ACAACATTCATTTTCATTCTCACTCCCCACCAAAAAAAAAAAAAACAGAAGCCATGAACTCCTCGTCTCTTCTAACTCCT TCATCATCTCCTTCTCCACATCTTCAATCTCCTGCAACATTCGACCACGATGATTTCCTCCACCACATCTTCTCCTCCAC TCCTTGGCCCTCATCCGTTCTCGACGACACTCCTCCACCAACTTCCGATTGTGCCCCCGTCACTGGATTCCACCACCACG ACGCCGATTCAAGAAACCAGATCACTATGATTCCTTTGTCACATAACCATCCTAATGACGCTCTCTTCAATGGCTTCTCC ACCGGATCTCTCCCTTTCCACCTCCCTCAAGGATCGGGAGGTCAAACGCAAACGCAGTCGCAGGCGACGGCGTCAGCCAC CACCGGTGGTGCAACGGCGCAACCTCAGACAAAGCCTAAAGTCCGAGCTAGGAGAGGTCAAGCCACTGATCCTCACAGTA TCGCCGAACGGTTACGGAGAGAGAGGATAGCGGAAAGAATGAAATCTCTTCAAGAACTTGTCCCTAATGGTAACAAGACA GACAAAGCATCAATGCTCGATGAGATTATCGATTATGTCAAGTTCTTACAGCTCCAAGTCAAGGTACTAAGCATGAGTAG ACTGGGCGGTGCTGCTTCTGCTTCTTCTCAAATCTCTGAGGATGCCGGTGGATCCCACGAAAACACCTCCTCCTCCGGCG AGGCGAAGATGACGGAGCACCAAGTTGCAAAGCTAATGGAAGAGGACATGGGATCAGCCATGCAATATCTACAAGGCAAA GGTCTTTGCCTCATGCCCATCTCGTTAGCCACCACCATCTCCACCGCCACGTGTCCTTCTCGTAGCCCCTTCGTTAAAGA TACCGGCGTTCCTTTGTCTCCTAACCTATCCACTACAATAGTTGCTAACGGTAATGGCTCATCGTTGGTCACCGTTAAAG ACGCTCCCTCCGTTTCCAAGCCGTGATAACGGCCATTTGTCCATTTCATTTTCCCTTTTTTGGGTGGGAAAGAGAGAAAA AAGTTTAGAAGACAAAGACAAGTGGGATAGGTGGTTTTGGTCAAA ............................i................................................N.. ..............................N...........i..........................N.......... ................................................................................ .....................................................i.......................... .................................................N.............................. ................................................................................ ..........................i...........................i..............i.......... ................................................................................ ................................................................................ ......................................i...........................N............. ............i......N..............N.....................................i....... .........................................N...................................... ........i..........................i...........N...........i.................... ............N................................................................... .....................................................i.......................... ................................................................................ ............................................. Pos Score Pred ------------------------ 29 0.679 Yes 78 0.382 - 111 0.210 - 123 0.555 Yes 150 0.215 - 294 0.757 Yes 370 0.379 - 507 0.520 Yes 535 0.658 Yes 550 0.586 Yes 759 0.698 Yes 787 0.313 - 813 0.525 Yes 820 0.103 - 835 0.395 - 873 0.848 Yes 922 0.462 - 969 0.704 Yes 996 0.507 Yes 1008 0.487 - 1020 0.503 Yes 1053 0.461 - 1174 0.676 Yes

Explain the output -- Processing locus 672: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATATTAAAGAACAGGTACTACCTATATACCTAATAGATTCCTCTAACCCGTCAAATGTCAATGTATCGACCGTTACAA TTATGCACGTCTTCTAATTGAAACATTCTCTACTTTGCCAATGTCTTATCCCCAAAACTACATATAAATATGCATTTACG GGGGCTTAGAATTTCATCACAAAACTTGCAAGTATCATTCACAGGAGAGGGAAGGGAGAGCTCATCATACGAAATGAGGT CAATCACAGCTTTGTTTTTCCTTTTCTGTTTCCTTGCTCCTTCTGCTTTGGCGCAGCTCCGAACTGGGTTCTACAGCCGC TCATGCCCCCGTGCCGAATCTATCGTGGCTAGTGTTGTTGCCAACCGTTTCAGAAGTGACAAGTCCATTACTGCAGCATT TCTTCGTATGCAGTTTCATGATTGCTTTGTCAGGGGTTGTGATGCTTCCCTCTTGATCGACCCAAGACCTGGAAGGCCAT CAGAGAAAAGCACTGGACCAAATGCAAGCGTGAGAGGCTACGAGATCATTGATGAGGCTAAGAGACAGCTCGAGGCTGCA TGTCCCCGAACTGTCTCATGCGCAGACATTGTAACTCTTGCCACAAGAGACTCGGTCGCATTAGCTGGTGGTCCAAGGTT CTCAGTACCAACAGGAAGACGTGATGGATTAAGGTCAAACCCCAATGATGTGAACTTACCCGGACCAACAATTCCGGTTA GCGCATCTATCCAATTGTTTGCAGCTCAAGGTATGAATACCAACGATATGGTTACACTTATTGGTGGTGGCCACAGCGTC GGTGTTGCACATTGCAGTCTCTTCCAGGACAGGCTTTCCGACCGTGCAATGGAACCCTCATTGAAGTCTAGCTTGAGGAG GAAATGCAGTTCCCCTAACGACCCAACAACGTTTTTGGACCAAAAGACTTCATTTACTGTGGATAACGCAATCTATGGAG AGATCCGAAGACAAAGAGGAATTCTAAGAATTGATCAAAACTTGGGTCTTGATAGATCAACCAGTGGGATTGTGTCTGGT TATGCATCAAGCAATACACTCTTCAGAAAGAGATTTGCCGAAGCATTGGTGAAAATGGGTACCATTAAGGTTCTTACCGG ACGTTCTGGAGAGATCAGGAGAAACTGCAGAGTCTTCAACAACTGAGGACGTTGATTCTTGCACACATTCTTTACAATTA TTCACGGAGAGATCCAAAGACTGAGCGGAATCCTACGTTTTTTCTTTTCTTTGCTTTTTTTCTTA ........................................................N.....N................. ..N.....................................i............................i.......... .........................................................................i...... ................................................................................ ..N............................................................................. .......N.........i.......................N...................................... .....................N.............................N...........................i .................N.............................................................. .......................N....................N..N................................ ................................i..............i................................ ................................................N............................... ...i......................................................................N..... ................................................................................ ................................................................................ ................................................................. Pos Score Pred ------------------------ 57 0.220 - 63 0.290 - 83 0.242 - 121 0.631 Yes 150 0.645 Yes 234 0.756 Yes 323 0.181 - 408 0.443 - 418 0.695 Yes 442 0.457 - 502 0.424 - 532 0.378 - 560 0.578 Yes 578 0.383 - 664 0.454 - 685 0.121 - 688 0.345 - 753 0.806 Yes 768 0.808 Yes 849 0.436 - 884 0.584 Yes 955 0.481 - 1042 0.162 - 1095 0.591 Yes

Explain the output -- Processing locus 673: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGCATTTATTGCCAATTTAGAAAAGATTTCTTACCCTTAAATCGTAAACTAACACATTTGTCTAGTGGCTACACTGAACC CAAAAACGAAACAACCCCTAACAGTCCTTGGTTTTTCTGAAGTTCAGAGAAATGACGACGACGATGTCAACCCTTCCAGT GGTTTTGGTAGACGAGATTCTCGCTAGGGTTCCCATTACATCTCTTAGATCACTCCGATCAACGTGCAAGAAATGGGAGG CTTCATCGAAAACCAATCTTGTAGGTGGTAAAGCAACCGCAAGGAAAAGCAGTCACGTAGGGTTTATCCTCATTGGTGAC AAGATCTGTTCAATGAAGCTGGACCTAAATGGCGGCGATGACTTTGTTGACACATCAGTGAATCAAGTAAGTGCATTTGA TGGATTCGCGATATCTCAACTCTTTCACTGCGACGGCTTATTGTTTTGCATCTCCAATAACCACTACAGCAACTACACGC TCATGGTATGTAACATGTACTTGGGGGAAACTAGGTTGATTCAAAACCGTAGCTTGTTCGAGAGTTATCAGAACTTTTGC TCGTATGCTTTCGGATATGATAGCAGCAAAAACCGTAACCACAAAATCTTGAGAAATAATTCTGTTTCTGGTGGTTATGA AATCTACAGTTTAAAATCCGATTCATGGAAAGATCTTAATGTTGATCTCGAGAAATCTATACATCTTTGGCGACTAGGAA GCGTGTCTTTGAAGGGAAATGCTTACTTTCGGGTTATAAAGGTAATTGAAGAAGGAGTTTGGGAATATAATTTACTCTGT TTTGACTTCACAAGAGAGAGTTTTGGAAAGCTTCTGTCTCTGCCGTTTGAGTCTTTGGGTGATGAAGGAGAAGGCATTAT GGTTATATCTTGTGTTAGAGATGATCATCTTGCTGTGTTATATCAACGTGACACTTTAGGTATTTGGATTTCGACTGAGA TCGAGCCCAACAAGGTGTCGTGGAGAGAGTTTTTGCAAGTGGACTTGGCGACGCTGGATGGTTTTCCGGATGTATTTATA GCTGGGAGATTCATCGTTGACGAGGAGAAGCAAGTCGTTGTAGTTTTCGGTCAAGAGACAGAGTTGGATCTCAACCACGG AAATGCTTTCATCTTTGGAAGAGATGGATACTTCACCTCTTTTACCGTTGGAGATGCTCCGCCCGAAGACTTCACTTCTT ATGTTCCAAGCTTAGTCTCATTGCAAATCGACAAGACGGGTAAAAGGAAAGCAAGAGATGACTAGATTGTTTCTTATGTT TCTTATCTCTCTTTTGTTTCAGTCATTTACTTTCATCTCCTTCTGAACAATTCTGCTTTTTCATGTTTATTTGGGATATT TGCT ................................................................................ ...................................................i...........N................ ........................................................................N....... ................................................................................ ............N...............i........N.........................................N ................................................................................ ..N....N......i................................................................. ....N...........N...........................................................N... ........................N.............N......................................... ..................N............................................................. .............................................................N................i. ....................N........................................................... .........................................................N...........N.......... ................................................................................ ..N....................N.............................N.......................... N........................................................N.................N.... ..............................................................N................. .... Pos Score Pred ------------------------ 132 0.776 Yes 144 0.446 - 233 0.422 - 333 0.481 - 349 0.514 Yes 358 0.283 - 400 0.273 - 483 0.453 - 488 0.170 - 495 0.505 Yes 565 0.257 - 577 0.184 - 637 0.203 - 665 0.229 - 679 0.151 - 739 0.203 - 862 0.298 - 879 0.674 Yes 901 0.124 - 1018 0.336 - 1030 0.104 - 1123 0.165 - 1144 0.321 - 1174 0.354 - 1201 0.095 - 1258 0.340 - 1276 0.079 - 1343 0.044 -

Explain the output -- Processing locus 674: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGAGTGTACACGCTTTCCACTAAAATTTAATTAATTAAGGGTTTGCTTACTCTAAAATTAACTAATTAAAATTCGTATA ACCAATTTTGTCATATGCTAAATTTTAATATATATGGGCTTTACAATACCCAAAAATCTTCGTATATGGACTTTCAAAAG CCCAAAAACACTTATTTCTGAGATTTTTTTTTCTAAGTTTTATTTATTGTTTCAGCTTCTTCTACAGTGCAACAGAGCAA CAATGGATTTTTTCGACGAAGACAGGCCTAGATTCGTTTTCCAATCTCGTCCTTCTTCTTCTCACACGGCGGAGGAAGAA GAAGAAGCTAGAATCCCTAACAAGCTTTTCATTTCAATCTCCGTCTCAATCTCCCTCATCATATTATCTCTCTCCTTCTT CTACTTCGAATCTGAACCTGCCAAATCGCTACTATTATGGCTTTCCCTCTCTTTCCTCGTCGGTCCTTTCGCTCCTAGCT CGCTCACCGGAGGTAAGATTCGCGTCGGTTATGGTCAGATCTTGGAGCCAGAACAGATTCACGATGAGTCATCCACGGAT AACGAACGTGAATCCAGGAGGAAATCGGTGAATAAGCGATCTAAAGGTTCGACGAAGAGCGATAATCCGCCGGAAAATGC TTCTGCGGTGACTGAAGTTTCGAGGAAAGTGGTGATTCCGCAGTCTAAGGAGAGTGGATCTGTGAATGAAACTAAAGATT GGACTGCTGAAGAGATCGAGATTTTGAAGAAGCAGTTGATTAAGCATCCAGCTGGTAAACCTGGGAGATGGGAGACGGTG GCTTCAGCGTTCGGAGGAAGATACAAGACAGAGAATGTGATTAAGAAGGCGAAAGAGATAGGTGAGAAGAAAATCTATGA GAGTGATGATTACGCTCAGTTTTTAAAGAATAGGAAAGCTTCAGATCCTAGATTGGTTGATGAAAACGAAGAGAATTCTG GAGCTGGTGGAGATGCGGAAGGTACTAAGGAGATTTGGAGTAATGGAGAAGACATTGCATTGCTCAATGCTTTAAAAGCT TTTCCTAAGGAGGCAGCTATGAGATGGGAGAAGATTGCAGCTGCAGTACCTGGGAAGTCGAAAGCAGCTTGTATGAAGAG AGTTACTGAGCTTAAAAAAGGGTTTCGGAGCTCGAAAACTCCAGCTAATTAACTAGAGGTAATTGATAAACTAGAGTCTT TTGATTGAAAAAGATACTCTTTATTAAGGTAATAAACATGCCACAAGAGCCTTATTCCATTGTAGTTGCTA ................................................................................ ..............N..................N...............................N.............. ................................................................................ ..i............................................................................. ................................................................................ ....................................i........................................... ..............................N................................N................ ............................................................................N... .................................................................N.............. ...................................................................i............ ..................................N.........................................N... .....N.....................................................N.................... ............N.............................N.......................N............. ..................i....i................................................N....... ................................................................................ .....................................N................................. Pos Score Pred ------------------------ 95 0.283 - 114 0.203 - 146 0.390 - 243 0.841 Yes 437 0.644 Yes 511 0.453 - 544 0.319 - 637 0.162 - 706 0.218 - 788 0.766 Yes 835 0.140 - 877 0.317 - 886 0.097 - 940 0.127 - 973 0.483 - 1003 0.215 - 1027 0.106 - 1059 0.562 Yes 1064 0.746 Yes 1113 0.219 - 1238 0.085 -

Explain the output -- Processing locus 675: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTGACCATACGTAACTGTACCATACTTTTATGTTTCCACCATCAGGGCAACACAATAAAATTGAATGCCATCTAAAACA AAGCAAAAAGATTTACTATAATTATTTTCTTACTCATGCATGTACTCCCAATCTCCCATAGATATTTTCGAAGGTATTTT TGTGGTAACGTACAAGTCTTGGCTTCGTAATCTAAATACCGAAAAAGAAAATGCCTCTAACTTCCCTTCCTCTCTCTATA TATTTCAACCACTCTCTGTCTCTCTTCCTCTTTGTAGTTTCTCAAATATTTTCTTCAGAGTTGGCACGACCTCATTAATA ATAATCTTCTCTACACCTATCAATAAGAGATATATTTTAGAGTTTCACTTTGGTGCACAAACAAAGCTCGTACAACATTC TTCGTATATGGCTCTTCGTCGCTGCTTACCTCAATATTCAACAACTTCATCTTATCTCTCCAAGATCTGGGGATTTCGTA TGCATGGGACCAAGGCAGCAGCTTCTGTTGTAGAAGAACATGTCTCGGGGGCAGAACGTGAGGATGAAGAATATGCTGAT GTAGATTGGGACAACCTTGGATTCAGTCTTGTACGGACAGATTTCATGTTCGCCACCAAAAGTTGCAGAGACGGAAACTT CGAACAGGGTTACCTTAGCCGTTACGGCAACATCGAGCTCAACCCTGCTGCTGGAATTCTCAACTATGGCCAGGGACTAA TAGAGGGGATGAAAGCGTACAGAGGAGAAGACGGTAGGGTTCTTCTCTTCCGTCCAGAGCTAAACGCGATGCGTATGAAG ATAGGAGCTGAGAGAATGTGTATGCATTCTCCTTCTGTTCATCAGTTTATTGAAGGTGTTAAGCAGACCGTTCTTGCAAA CAGGCGTTGGGTTCCTCCTCCGGGCAAAGGCTCGTTGTATCTCAGACCGTTGTTGTTCGGAAGTGGAGCAAGCTTGGGTG TGGCTGCAGCATCAGAGTACACGTTTCTTGTGTTTGGCTCTCCTGTTCAAAACTACTTCAAGGAAGGCACAGCGGCGTTG AACCTGTATGTGGAGGAGGTGATTCCACGCGCTTATCTTGGAGGAACTGGTGGTGTAAAGGCAATATCCAATTACGGTCC AGTGCTTGAAGTGATGAGAAGAGCAAAATCAAGAGGGTTTTCGGATGTTTTGTATCTTGATGCAGATACTGGGAAGAACA TAGAAGAAGTCTCTGCTGCTAATATATTCCTTGTGAAGGGCAATACAATAGTGACACCAGCTACGAGCGGAACGATTCTC GGAGGGATCACACGTGGAAGAACGAAGTGTTCCGGTAGAAGAACTGAAGGAAGCTGAAGAAGTTTTCTGCACTGGAACTG CCGCTGGAGTTGCTTCTGTTGGAAGCATCACTTTTAAGAACACAAGGTCTCTTCTTCCTCTCTGGATACATGTATA ..............................N..................................N.............. ...................................N...N........................................ ..................................................i............................. ................................................................................ ................................................................................ .......i.......................................................................i ...N...................................i.......................N........N.....N. .............................................N.................................. .................................................................N.............. ........i...........................................................i.....N..... ...............i.....N.......................................................... ................................................................................ ................................................................................ .......N........................................................................ .............N..............................N..............N.................... ................................................................................ ................................................................................ .....................................................................N...... Pos Score Pred ------------------------ 31 0.239 - 66 0.279 - 116 0.244 - 120 0.393 - 211 0.548 Yes 408 0.593 Yes 480 0.904 Yes 484 0.400 - 520 0.530 Yes 544 0.403 - 553 0.434 - 559 0.428 - 606 0.310 - 706 0.448 - 729 0.743 Yes 789 0.505 Yes 795 0.492 - 816 0.511 Yes 822 0.447 - 1048 0.143 - 1134 0.437 - 1165 0.080 - 1180 0.123 - 1430 0.043 -

Explain the output -- Processing locus 676: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGTCTTTCTCTCTGCTAGCAATTCCTGGGCTAAATTGGATGATGATATTGTGTTAAAGAACTCGAAGATGATTTGTCA AAATTAAACTTGATTCTGTAGATCCGTGAGTTGAATCAGATATTAACGTGTCTCTATGTTTCTATGTATCAATTTGGTTT GATAAGGTTACTTAGAATGGCACAAGAAGCCGATGGGATCAGATTGGACCAAAGACATGGGAAGGCTCGAGTGAGAGTTG GAAGAGTTTGGCGTCATGCTCATGATGGATCTCATCACTTTGTTGAATGGAATGTTAGCATCAGTCTTCTCTCTCACTGT CTCTCTTCTTACCGTCTTGATGATAACTCTGATATTGTCGCCACAGATACCATTAAAAACACTGTTTATGTGAAAGCCAA GGAATGTGGAGATCGGCTCTCGGTGGAGGAATTTGCCATACTTATTGGGAAACACTTTTGCTCATTTTATCCACAGGTAT TTACTGCTATCGTGAATATCATTGAGAAGCCCTGGGAGCGTGTCTCCATCGATGGAAAACCACATTTACATGGTTTTAAG CTTGGGTCAGAGAACCATACTACAGAGGCAAGAGTAGAAAAGTCTGGTGCATTAAACTTAACTTCCGGTATTGGAGGACT AGCTCTACTGAAGACAACCCAGTCAGGATTTGAGAGGTTTGTCAGAGACAAATACACCATTCTGCCTGAAACTCGTGAGC GAATGCTGGCCACAGAGGTTAATGCATCTTGGAGGTACTCGTATGAGTCAGTTGCAAGCATTCCAACAAAAGGACTCTAC TTTAGCGAGAAGTTCATGGACGTTAAGAAAGTTCTGATGGATACTTTCTTTGGTCCACCAGAAACTGGTGTGTATAGCCC TTCTGTCCAACGCACTCTCTACCTCATGGGAAGCGCCGTACTGAAAAGGTTCGCTGATGTATCATCGATTCACCTAAAAA TGCCAAATATTCATTTTCTACCAGTAAATCTTTCAACGAAGGAAAACCCTTCAATGGTGAAGTTTAAAGATGATGTGTAC CTGCCAACCGATGAACCTCATGGATCTATAGAAGCAACTCTAAGCCGTATAACCTCGAAACTATGAGGTTTTAAGACAAT ACATCATACATGGAAATATCAAACACAATTTCACAGGTTCTGCAACTTTTGGCGTTTGCCTCGTTTATGAAATAACAATT TAAAT ........................................N..N.........................N.......... .......................................................N.......N................ ................i...............N.......................N....................... ...............N.....N..N.....................N....N............................ ...................N...............................................N............ ...N............................................................................ ...................................................N.................N.......... ................................................................................ ................................................................................ ..N..................N....................N..................................... ...............i....................i........................................... .........................N..............................N......................i .....................................................i...............i..N....... ..........N........N..........................................N................. .........N........................................................N............. ..... Pos Score Pred ------------------------ 41 0.281 - 44 0.122 - 70 0.269 - 136 0.296 - 144 0.332 - 177 0.648 Yes 193 0.405 - 217 0.346 - 256 0.166 - 262 0.183 - 265 0.261 - 287 0.121 - 292 0.070 - 340 0.219 - 388 0.295 - 404 0.423 - 532 0.365 - 550 0.221 - 723 0.357 - 742 0.263 - 763 0.094 - 816 0.809 Yes 837 0.739 Yes 906 0.399 - 937 0.128 - 960 0.686 Yes 1014 0.671 Yes 1030 0.690 Yes 1033 0.351 - 1051 0.307 - 1060 0.288 - 1103 0.375 - 1130 0.177 - 1187 0.026 -

Explain the output -- Processing locus 677: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTGGGATATTTGCTGCAAAAACGATGCGGTTCGAGTAAGAATGGCCACGTGGCACAATCTATCACATCAAAACGACAC CGTCAGAATTCATCATGGTAAACGAACGGTCATGATTTAATCGCGATCCAACACAGGAAAGTTTATGAGTGTGCAATGAC GAATGTATCCCTACAGAGTTCAGAATATCGACGGCGACAATTTCTCTCTCCGGCTAACTTTTTTCTAGAGAAAGCTATGG CGGACGAAGAAGATGAGTTACTGGAGACAGAACTAAGTTACGACCAGAAAAAGGAAATCGCCAAGTGGTTTTTCCTCAAC GCTCCCGCTGGAGAAATCAATTACGTCGCTAAAGATTTAAAGGCGGTTTTGAGCGATGAGGAAGTGTACAATGAAGCAGC CATGGAAGCATTTCCAGTGTACAACAAAACTCACATGATTTGCCTTGAAATGCCCAGTGGAGCTGGTGATGTGATTGTCT CATCTTACAGTGAAATTAATGAAAATGAGTACCTTGATCCAAGAACTGCCCAGGTTGCCATAGTTGATCATGTTAAACAG ATTTGTACAAAGGTACGGCCTGCAAATGATGAAGAACTTCCTTCTTTGTATATTGAGGAGTACAGATATGCTCTTGATGC TGAAATCCAGAGATATGTTAGTGAATCTTATCCGAAAGGTATGTCTGCAGTTAATTGTGTGAAAGGGAAAGATAATGAGG GTCCAGGGTCCGACTTTGAGCTTGTTGTTATAATTACTGCTATGAGACTTAGTCCCCAGAACTTCTGTAATGGAAGTTGG AGATCTGTATGGAACATAGATTTCCAGGATGAATCCCAGGTGCTTGACATAAAAGGGAAATTGCAGGTAGGAGCTCATTA TTTTGAAGAGGGAAATGTGGAATTAGATGCAAAAAAAGATTTCCAAGATTCAACAATATTTCAGTCTGCAGATGACTGCG CGATTGCCATAGCCAATATTATCAGGCACCATGAAACAGAGTACCTTGCATCTCTAGAGGTAGCTTATTCAAAATTACCG GATAACACTTTCAAGGATTTGAGGAGGAAACTTCCGGTGACACGAACTCTGTTCCCATGGCAGAACACTTTACAGTTCAG CCTAACAAGAGAGGTCGAGAAAGAACTTGGACTTGGCAAGTGACTAATCATCAACTCGCAACCTTTTGAGTGCTATATTT CGTTGTCAAAGTTTCTGTGATTTCCATTATGTAAGTGAAGGATAAGTATTATTTCTGTTGTG .........................N................i..................................... ..............N................N................................N..........N.... ..N.........................................................................i... ............N................................................................... .......................................................N..............N......... ..................N.....N............................................N.......... .........................N..N......................................N........N... ..............N.........................i.................................N..... .........................................N...........................i.......... ........N...................N................................................... ..............N...........N............................................N........ ..............................N................................................. ........................................................i....................... ................................................................................ ............................N................................. Pos Score Pred ------------------------ 26 0.384 - 43 0.710 Yes 95 0.459 - 112 0.426 - 145 0.467 - 156 0.458 - 163 0.451 - 237 0.841 Yes 253 0.354 - 376 0.179 - 391 0.383 - 402 0.465 - 435 0.473 - 450 0.686 Yes 469 0.162 - 499 0.111 - 505 0.153 - 550 0.150 - 586 0.177 - 589 0.152 - 628 0.214 - 637 0.077 - 655 0.144 - 681 0.752 Yes 715 0.450 - 762 0.375 - 790 0.501 Yes 809 0.166 - 829 0.141 - 895 0.417 - 907 0.133 - 952 0.213 - 991 0.144 - 1097 0.677 Yes 1229 0.041 -

Explain the output -- Processing locus 678: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCTTGTAAGTAAATTAAGTCTCATACTTTCATGTAATGCCAAAAGAATTAAAGAAGTGATAGTGAACACACATCTCAA ATTAAAGGGACTAATTTGTAAGAAATGGAAAGAAAGGGGTTTATTTCACAATTCTTCTCGTTTTGCATATGTCATCATCC TTATCCATTTTTCACCTTATCCAAAATATTTTCCACTTCCTTTCTCACTACTTCAGTCTTCAAGCTTCACTTCTCAGAAG AAAGTAAGATCTGCGAATCTCTCTCACTCTCACAAATGGCAACGCTTTTAGGTTTCTCTCAGACTAAGTTTCACCATTGC GGCGTCTGGATTTCTACGCCGCCGTGCTCATCTTCTTCCACGGTAGTCTCAATGGTGGGCTTGAATCGGACTGATTCGAA AAAGCTCCGTTCAGATTTCCTCGGCCAGATTGGTTACGAAGATCGGAGACAAGTAAGACACAGTTACTGTAGAAGCTCAT TAGCTGTGAAAATGTCGTGGGACGGTCCTCTTGCTTCCGTCAAGTTGATCATCCAAGGAAAAAACCTCGAGTTATCAGAG CCAATTAAGCAGCATGTTGAAGAGAAAGTAGGCAAATCTGTTCAGAAACACAGTCATCTTGTGAGAGAAGTTGATGTAAG ACTCTCTGTTCGTGGTGGAGAGTTTGGTAAAGGCCCTAGGATTCGAAGATGTGAGGTGACATTGTTTACAAAGAAGCATG GTGTTGTGCGTGCTGAGGAAGATGCTGAGACAGTATACGCTTGTATCGACTTGGTATCAACGATAATACAGAGGAAGCTG AGGAAGATCAAGGAGAAGGACTCAGACCATGGAAGGCACATGAAAGGTTTCAACAGATTGAAGGTAAGGGAACCAGTGAT TGAGCCGGTTGTGGAGGATGTTGAGGACAGTACTGACTCGAGCGTAGGAGAAGAAGAAGAAGAGGATGATTTGATCAAGG AGATTGTCCGTACCAAGACTTTCGAGATGCCACCATTGACTGTCGCTGAGGCAGTCGAGCAGCTGGAACTAGTCAGTCAC GACTTCTATGGCTTCCAAAATGAAGAAACTGGTGAGATAAACATAGTGTACAAGAGAAAAGAAGGAGGTTACGGTCTGAT AATCCCAAAGAAAGACGGGAAGGCCGAGAAGGTTGAGCCGCTTCCAACCGAGCAATTGAATGAACACTCTTTCGCCGAGT AGACTGCCTCTGCTCACACCAAAACCGATAAGCTCATCTCTCCTTACAGTTTACCTGTGTAGGAGTTAGGGTTCTTGAAT AAACAATGCAACAAAGATTGT ................................N....N.......................................... ........................N...........................................N........... ................................................................................ ...................................i............................................ ...................................................N............................ ................................................................................ ...........i.................................................................... .............i...........................................................N...... ................................................N............................N.. .....................N.......................................................... ............................N..........i........................................ .................N...............................................N.............. ..........................N..................................................... .......i...........N............................................................ ...........................................................N.................... ................................................................................ .....N............... Pos Score Pred ------------------------ 33 0.175 - 38 0.311 - 105 0.192 - 149 0.174 - 276 0.628 Yes 372 0.459 - 492 0.915 Yes 574 0.575 Yes 634 0.244 - 689 0.318 - 718 0.228 - 742 0.243 - 829 0.492 - 840 0.685 Yes 898 0.157 - 946 0.147 - 987 0.474 - 1048 0.551 Yes 1060 0.336 - 1180 0.091 - 1286 0.078 -

Explain the output -- Processing locus 679: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAGCTCTTTATAAATCTCTATCTTCTCCTATTTCTTTTTCACCATCTTTTCTTTCTCTCTCTCTCTCTCTTTATATAAG TCATTCATAAAATCACAATAAACACAACAAAGCAAAAAGGGTAAAATGAGCATTCAATTCAATATGACTAATGCTCAAGA GTTGGGGCAAGAGGGTTTTATGTGGGGCATATCCAATTCTGATGATTCCGGAGGTGGTTGCAAAAGAATCGAGAAAGAAC CGCTTCCATCACATCCGTCTCATCCGTCGCCAGAGATCCAGACTACGACGGTTAAAAAGGGAAAAAAGAGAACGAAAAGA AATGATAAAAACCATGAAGAAGAATCACCTGATCATGAAATACATATATGGACCGAAAGAGAAAGGAGAAAGAAGATGAG GGACATGTTTTCTAAATTACATGCTTTGCTTCCCCAACTTCCTCCTAAGGCAGACAAATCAACAATTGTAGATGAAGCAG TGAGTTCAATCAAATCCCTTGAACAAACTTTGCAAAAACTTGAAATGCAAAAGCTTGAGAAGCTTCAGTATTCTTCTGCC TCAACAAACACAACTCCTACTACAACTTTTGCTTATGCTCCATCATCATCCTCTTCTCCAACAGCTCTCCTCACACCAAT ATCAAACCATCCCATAGACGCCACCGCAACAGATTCTTACCCTCGCGCAGCTTTCTTGGCCGATCAGGTATCTTCTTCCA GTGCCGCTGCTGCGAACCTGCCTTACCCTTGTAATGATCCGATCGTGAATTTCGATACTTGGTCCTCACGTAACGTGGTG CTGACTATCTGTGGGAATGAAGCTTTCTTCAATTTGTGTGTCCCTAAACACAAACCAGGGGTTTTCACTTCTGTTTGTTA CTTGTTTGAGAAGTACAACATGGAGGTTTTGTTTGCTAATGTCTCCTCTAATGTTTTCTGGAGCACCTACGTGATCCAAG CACAGGTAAATCCGAGTTGTGAGAATCAGTTACTGGGAAATGGTCTTGGAGTTGTAGACGTTTTCAAGCAAGTTTCTCAA GAACTGGTGTTATATTTTTCATCTCTATAAATCATAACTGGCTGCTTCGGTTTGAGTTTTAAGTATTTTCAGGGTTTGTT TTCTTTGCGAAGTCCCAAGGCTTATGCAGAGAGATTAGCTTGGACTTTT ................................................................................ .............................................i.................i......i......... ...................i.....................N...................................... ................................................................................ ....i...............N..................................................N........ ............................................i................................... ..................................N............................................. ................................................................................ .................................N.............................................. ................N............................................................... ...................i..................N...........N............................. .......................................N........................................ ................................................................................ .......................N......................... Pos Score Pred ------------------------ 126 0.806 Yes 144 0.701 Yes 151 0.525 Yes 180 0.553 Yes 202 0.240 - 322 0.496 - 334 0.252 - 355 0.104 - 368 0.501 Yes 396 0.711 Yes 405 0.584 Yes 421 0.149 - 472 0.278 - 525 0.582 Yes 595 0.376 - 754 0.120 - 817 0.429 - 900 0.728 Yes 919 0.322 - 931 0.239 - 1000 0.241 - 1144 0.042 -

Explain the output -- Processing locus 680: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATCAAAACGTCTAATTAATATACAGTTGTATACACAATCGTGTAAAATTGAAACAAGAAAGTACTATAACGTCTAATA AATAGGTGTATGCTCGTGTAAAATTGGAAGAACAAAATAGATACGTATAAAATGTATATCTTAACCGAACTAAATAAAAA TATAGTTGGGGAAATAAGAAAGCAATTATTCTCAAAAAATAGCGTAACTCTCCCACGTGCACATCATCTCATACTTCAAT TGCCGAAACCCCACCAACTTGCGTATATAAACGCTCCTTGTTCCTCATCTTTCACCATTTCAAAATCAAACCATCACAAC AACTCTCTCTTCTCTCTCTCTCCGCCGAGAATGGTTGGAGTTTTCCGACGATCACTCTCATTCCCTAACAAACCTTGCGG CCGATCATCTCCATCGTCAAAGCCACGTGTCTCTCATCACACAAGATCCATCAGTCTACCCTGCCGGTCTCATCCCTTAA TCTCCCACGTTAACCACGAGATCTCTCAGCTCAAATCCTGGTTCTCATTCGCCGGAGAAACTCACAGCCGCACCACCTCT TGGATCACCGACGGTCTCAGTCTCCTCAAGGACGTTCAAGAAACGCTCGCTGATATACTTCAGCTCCCACAATCTCAAGA ATCTCTACGAAACCGTCCTGTTTTCTTCGAGAATCTTCTAGAAGATCTTCTCCGATTCGTTGACGCTTACGGAATCTTCC GTACGTCGATCCTCTGCCTCCGTGAGCACCAGTCCGCCGCTCAAGTCGCTCTCCGTAAAAAAGACGACGAGAAAATCGCA TCTTACCTAAAATCTCGCCGATCTCTCGCAAGAGATATCGCGAAACTGACGTCATCGATCCGTGAGCCGAAGACAAAACA CCAACACTGCCACGTAGACAACGTTAACGGAACGTACGGTGACGCAGAGCTTGCGTCAGTTATAGGTGACGTCATTGAAG TAACTGTTTTGGTTTCCGTTGCTCTCTTTAACGGCGTTTACTTATCTCTCAGAGCCACCAAAACAACGCCGTTTATAGGG TTTTTGAAACGATCCGAAAAAAAGGAGAAACTAGACGAAGGAATCGTGGAGTTGAAGCAAGTTGAAGAGAAAAGCTTGAT TGGTTTAAGCAAGAAGAAGAACGAAGAAGTAAAGAGTTTGATGAAGAGGATGATGGAATTGGAGAATTCGATCCGTGAAA TCGAATGTGAGAGTGAGAAAGTGTTTAGGGGTTTGATTAGCACTCGAGTCTCATTGCTTAATGCCCTAACACATTAACTA ATTATCACTCTCATTCTTTTTCTTCTTGTTTCCTGTCTATTTGCGTTATTTTGTCACATTATTTTTATTATTTGTATGAG GTTAACTACATGAAAG ................................................................................ .........N.........................................N............................ ................................................................................ ................................................................................ ..............................i................................................. ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ........................................N........N..N........................... ....i.......................................................N................... ...........................................................................N.... .........N...... Pos Score Pred ------------------------ 90 0.126 - 132 0.398 - 351 0.501 Yes 1161 0.255 - 1170 0.226 - 1173 0.268 - 1205 0.537 Yes 1261 0.096 - 1356 0.038 - 1370 0.055 -

Explain the output -- Processing locus 681: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTATATTTCCCGGTGATTTTTGTTTACACGTACAAGTGGAGCCATGAGTGAGACCAAAGTGGGCTGTTGACGTCATTC GGAATATACCAAAAGTCATAGCACGCACAAAGACGCTCAAAGAGAGACAGAGAGAGAGCTTATCTAGCAAAAAAATCAGA GAGAATCAGACAATGGAGAAAAAAAAAACAAGGAACTCGCAAATCAATGAATCTGCATGTTTTTTTCTGGCCTCTGGGTG GTTCCTTTGGAGGTTTCTGGCTAAACGACATTATGTTTCTTTTTTCTTTCTTTCACAAATATCTGCCTTTTTCTCCTGAT AAAAGGTACAATTCACAAATATCTAATATGTATATATATAGAGAGTATTTGTGTTTTGAGGGTTCTCTCAGAAACTTTGT TTCTTTTGTCTCAAGATATGAATCATTTTCGTTCAAGACTTCAACTTGGAAACCTATGATGATTATATCATCACAGATTC TTCTTCTTTTTGGTTTTAAACTTTTTTTTGAAACTAGGGGTGAAGATGATATAGTGGAGCTCTTATGTAAGATTGGCCAG ACACAGATACCCTCCTCCGATCCTCTTCCCATTCTCCGTGGCAGCGGAAGTGGCGGACGAGAAGAAAATACTCCGCTTCC GCCTCCTCTGCCTCATCAGAATCTCTTCATCCAGGAAGACGAAATGTCTTCTTGGCCCCATCATCCACTCCGTCAAGATT ATTTGTGCTCTGAACTTTATGCTTCCACTCCGGCTCCTCATCCGCAGAGTTCCGTCTCTCTGGCACCACCACCACCAAAA CCACCATCCAGTGCTCCGTACGGTCAGATTATCGCTCCAAGAAGTGCTCCGAGAATCCAGGGAACTGAAGAAGCTCGAGG TTCAACGTCTAGGAAGAGGTCACGAGCTGCAGAAATGCATAACCTCGCCGAAAGGAGACGGAGAGAAAAGATCAACGAGA GAATGAAGACTCTGCAACAACTCATTCCTCGCTGCAACAAGTCTACTAAAGTTTCAATGCTGGAAGATGTTATCGAGTAC GTGAAATCTCTAGAGATGCAAATAAATCAGTTCATGCCCCACATGGCCATGGGTATGAATCAGCCTCCTGCATACATACC TTTCCCTAGCCAGGCTCATATGGCGGGTGTAGGTCCATCATATCCACCACCGCGATACCCTTTTCCAAACATTCAGACCT TTGACCCATCCAGAGTTTGGTTACAAAGTCCACAGCCTAACCCGGTGTCGAACCAACCTCAGATGAATCCTTATGGTCAG TTTGTTGGTCACCATCAGATGCAACAATCTCTTCCTCCTCCATTGCAGGTAATTCTTTCCCAATATCCATTGTGCTTATT CCTCTGTTCCAACAAATGACGTTGCTCACAAGACCCATCCTCTTTTTAAAAATGTTCTTATAGAGTCAACAAATAACATC ACAGCTGAGTTTAGGCCAGGCAGGTAGTAGCAAGGAAC .............................................i.................................. ................................................................................ ............i.................................N.........N....................... ................................N............................................... ...........................i.................................................... .................N.....................................N..i..................... .............................................i..................N............... ................................................................................ ...........................................i.................................... ..................N............................................................. ................................................................................ ..................................i............................................. ..i.....................................................N.........N............. ...............i.................N........N.....i.....N......................... ...................i............................................................ ..............................................................i.........N....... ..................N............................................................. ...............N...................................N............................ ...................................... Pos Score Pred ------------------------ 46 0.695 Yes 173 0.523 Yes 207 0.190 - 217 0.126 - 273 0.184 - 348 0.562 Yes 418 0.129 - 456 0.248 - 459 0.594 Yes 526 0.608 Yes 545 0.452 - 684 0.820 Yes 739 0.250 - 915 0.642 Yes 963 0.581 Yes 1017 0.442 - 1027 0.144 - 1056 0.841 Yes 1074 0.361 - 1083 0.480 - 1089 0.581 Yes 1095 0.278 - 1140 0.698 Yes 1263 0.584 Yes 1273 0.267 - 1299 0.261 - 1376 0.489 - 1412 0.083 -

Explain the output -- Processing locus 682: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGCTGATTTGAGTTCTTTTTTAAAAATCATTAGCTAAGTTAGTTTCAGAGCTCTTCTTCGAATATCTGGAAGAGAGACGA GTTTAGGTCGATCGATTTTGAAAATGGTATAGAGCTTCGTTTATTTGTGAGAACGACCAAGCTTTTTCTATTGTGATGTT ATGGTATAATGATATGCATAGAATTCTACGTGAACCAGGAATGGATCAATATGGGATTCCAGTAGAGAGAAAAGAGATGA AGCATAAGGGGAGAAATGTTATATGGTCTGTTGGAATGGATAAGTGTTTGATTGAAGCTTTGGCTGTCCAGGCCAAAAAC GGGAACAAAGTTGACAAATGTTTTAACGATAAGGCTTACACTGCTGCTTGTGTTGCGGTTAATACTCGTTTCAATCTTAA CTTGACTAGCCAGAAAGCCATCAATCGCCTCAAGACGATCAAGAAAAGGTACAGAGTTATGAGAGACATACTTAGCCGGG ATGGATTCTGGTGGAACTCGAGCACAAAAATGATCGATTGCGAAAGTGATGAGCTCTGGAGAAGATATATTGCTGTAAAC CCAGACGCAAAAGCATTTAGGGGAAAGCAAATTGAGATGTACGAAGAACTTAGAACAGTATGCGGTGACTATCAAACTCC AGGTAAATATAACAAAGTGAAGAAGGAAAGCAGTCATCATCTTAATGACGTAAAACAATTTGAAGAAGACTCTGTTTCCT TCCCTTTAGGAAGTTCTGAAGAACATAGCGATACAGATGGAACAGAGTCATACGCAGGAGCAAGTGAGTATATGCATGAA GAGTCTCAAGATCTTCCTCCTCCTCGAGATCCTTTAAGACGACCTTCTAAACGTTCTCGAAACTCGGATCCTTGTCAAGA AGCAATGTTAGTGGTTGCTTCTAGCATTCGCCGCCTAGCTGATGCAGTGGTTCAAAGCAAAACTTTGATTAACACAGAGG AATTGTTGAAAGCAGTGATGGAGATTGATGAATTGGAAGAAGCAAAACAGATGTATGCGTTTGAGTATCTGAATGGTGAT CCAGTCAAAGCTAGAGCTTTCATGGCTTATAATAATAGGATGAGGAAGATGTTTTTGTTTCGACAGTTTTGGTGGTGGAA GTAAAAAAATTGATTTGCTGCTATGGCTAAAAGAATCCTCCTCTTCTTTTGGGGTAATGTAGAGTCTTGTTAATGTTAAA AGCTGGAAATATTTGTTGAGGCC ................................................................................ .......................N...................................................i.... N.......N....N..........................N.........N.........................i... ...............N......N............i............................................ .................N.............................................................. ..........................................................i..................... N............................i..................N............................... ....................................i......................N.................... ............................................N................................... ....................................N..................................i...i.... ................................................................................ ....i....................................N...................................... .................i.........N......................N...N.................N....... .....................N.................N........N............................... ......................N.................................N...............N....... ....................... Pos Score Pred ------------------------ 104 0.499 - 156 0.619 Yes 161 0.332 - 169 0.178 - 174 0.364 - 201 0.483 - 211 0.366 - 237 0.704 Yes 256 0.160 - 263 0.167 - 276 0.753 Yes 338 0.442 - 459 0.711 Yes 481 0.212 - 510 0.716 Yes 529 0.317 - 597 0.557 Yes 620 0.137 - 685 0.355 - 757 0.394 - 792 0.509 Yes 796 0.616 Yes 885 0.796 Yes 922 0.130 - 978 0.644 Yes 988 0.247 - 1011 0.379 - 1015 0.151 - 1033 0.166 - 1062 0.388 - 1080 0.130 - 1089 0.180 - 1143 0.219 - 1177 0.072 - 1193 0.050 -

Explain the output -- Processing locus 683: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATAACACAATCTCTGGAATCTTGTTCATTTAATACACGTAGCACAATGGTTTTTATGAGAGAGAGACCATTTATAGGAT GTTGTAGGAGAATCTAAATAGATCTCTTTTGTACTTTCCTTTTTCTTACTCGTTATTGTACTGATGGGAGCCACTTGTGT GCATGGTTTTAGGCCTGACCACAATTTGCAAGACTTGAGGGCCAAAATCTTTGCTCTAAAACGAAGAAAAGTGAAAGCTC CTGGAATTGTGTCCTTACCAGGAAAGAGGAAGGAGAGATCTATATCTTCTTTGGTTGTGAGCACACCTATGGTGTCAGCA CAAGCTGGAACAACGAGAAGAAGAACTAAAGCACCTACAAGAAAAGAGTTACGTAATGGTTCTTTGGCTGAGAGAACTGT GAAGAAGGAAGAATCTTCTGGGGATGAGCTTCTGGAGAGCACAAGCTCACCCGACACTCTTAACAAATTCACTCAGAATA AAAGACAGTCAAAGAAGTCATGTAAAGAGTCCATCTCCAATAAAGAAAACAAGGATGGTGATGAACCCTGGGATTCCAAA ATGGATTGGAAACCTTTGAACTTTCTGGTTGAAGTGGCAAATGGGACAAAGCCCTTGAAGTCTTCTGCTTCTCAAGGGTC AGGTTCAAAATCTGAGCACGCGAATGTATCTCGCAATCAATTTCAAGGGAGTAAAACCAAAACTAAGAATAAAAAGAGAA AATGTAAACGTGAGGATGACAAGAGTAATAATGGTGATCCTACAACATCAGAAACTGTTACTCCTAAAAGAATGCGTACA ACTCAACGCAAAAGGTCTGCCACCACTTTAGGCGATTCAAGGAATTTGCCACAACCAGATGAGTCAAGTGCAAAACAGGA GAGGAGAAATGGTCCCGTTTGGTTCTCACTTGTGGCGTCCAATGATCAGGAAGGAGGAACTTCTTTACCTCAAATTCCAG CAAATTTTTTGAGAATAAGGGATGGAAATACAACAGTTTCTTTCATCCAAAAGTACCTCATGAGGAAGCTAGATCTCGAG AGTGAAAATGAGATAGAGATAAAGTGTATGGGAGAAGCGGTGATCCCAACGCTGACGCTTTACAATTTAGTGGATCTATG GCTCCAGAAAAGTTCAAACCACCAACGGTTTGCTGCATTGGTAGGTTCTTCTGCAAAGGATTTTACGATGGTTCTTGTCT ATGCTCGGAAGCTACCAGAGTGCAACATGTAAAGGACTTTAAAGACTCATGTTGTTGCAGAAGAAGATTCTGTGGAGACA GGGTAATCTTTTCTTTTCGGTTTATGGAATCTCTCAAAGCATTACAAGAGA ..............................................i........N......................N. ...............................................................N................ ..N............................................................................. ....................................................................i........... .......................................................N........................ .......................N........................................................ ...................N..................................N.....N................... i.......................................N....................................... .......................N........................................................ ..........................................................N..................... ........N................................N...................................... .....................N.....................................N.................... .......i...................N.................................................N.. ...................................................................i............ N.........................i.....................N............................... .......................N........................... Pos Score Pred ------------------------ 47 0.506 Yes 56 0.474 - 79 0.127 - 144 0.445 - 163 0.321 - 309 0.697 Yes 376 0.213 - 424 0.163 - 500 0.368 - 535 0.377 - 541 0.171 - 561 0.656 Yes 601 0.472 - 664 0.387 - 722 0.517 Yes 736 0.403 - 751 0.609 Yes 792 0.609 Yes 859 0.175 - 889 0.314 - 922 0.287 - 982 0.478 - 1020 0.393 - 1048 0.561 Yes 1068 0.303 - 1118 0.484 - 1188 0.718 Yes 1201 0.338 - 1227 0.577 Yes 1249 0.096 - 1304 0.052 -

Explain the output -- Processing locus 684: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAATTTTTGTTTGTTTCTCTCTAATTCGTGTTCCCAATTTTATATTCCTATGTTATGAATGACCATTAATTTATTTTTT AATTCTCTAATTCGTGTATAAATTAATTTATTGATAATGCATGCATCTGGGTTTTTTTGTTTGTGGGTGGTGAAGCTGGA GTGGAAGAAAGGGAAAGTGAACCCATTCGTGAGGGGAATAAGATCGAGGTTAATTAGGCCCAAATCGATGCCTTTAAATG TAGTCAATGATCGTGATGGCTCTTCTCGCATTCTTTTGACCGACCCTGCCGGATCAACTGCTGAGGTGCTTTTGTATGGA GGGCAAGTGGTTTCTTGGAAAAACGAAAGGAGAGAGAAGTTGCTTTATATGAGCACCAAGGCGCAATTGAAGCCACCAAA GGCGATCAGGGGAGGGTTACCTATCAGCTTTCCACAGTTTGGAAATTTTGGTGCACTTGAGCGACATGGATTTGCCCGTA ACAGATTCTGGTCACTTGATAATGATCCTTCACCTCTGCCTCCGGCTAACCAGCAATCAACTGTTGATTTAGTATTGAAG TCTACTGAAGATGATTTGAAGATATGGCCTCATAGCTTTGAACTTCGTGTTCGCATATCTATCAGTCCTGGAAAACTTAC AATAATCCCGCGTGTGAGGAACACTGACACAAAGGCATTTTCCTTTATGTTTTCACTTCGTAACTACTTATATGTATCCG ATATAAGTGAAGTTCGGGTTGAGGGTCTGGAGACGCTTGATTATCTGGACAACTTGATGCGTAGAGAAAGATTCACGGAG CAGGCAGATGCAATTACCTTTGACGGCGAGGTTGATAAAGTGTACTTGAACACGCCGACAAAGATTGCCATCATAGATCA TGAGAGGAAAAGAACTATCGAGTTGCGAAAGGAAGGCATGCCAAATGCAGCTGTGTGGAACCCTTGGGACAAGAAGGCCA AGAGTATTGCAGATATGGGAGATGAGGATTACACAACAATGCTGTGTGTAGATTCTGGTGCTATAGAAAGCCCAATTGTG TTGAAGCCACATGAAGAGTGGAAAGGAAGACAAGAACTCTCTATCGTCTCCTCAAGCTACTGCAGCGGTCAGCTCGATCC ACGTAAGGTTCTTTATGGGGATTACTGATAATATATATGAAATCAAACTTGGAAACATCATCAGTCTTATATTTTGATCG TCTTCATGGATAAAGCGATTGATGCCCAAACAAATCTGATATGATAT ..................................................N....N...N.................... ....................................i...N....................................... ...................................................................N.........i.. ......N........N...........................................................N.... ................................................N............................... .................................................................N.............. .....................N.......................................................... ..........i............i........................................................ ..............................................i........................N........ ........................................................i....................... .......N.......................................................................N .....................................i......N................................... ..............i......N................i......................................... ..........N..................................................................... ..............N.....................N........................................... .....N...............N..................N...... Pos Score Pred ------------------------ 51 0.188 - 56 0.370 - 60 0.460 - 117 0.620 Yes 121 0.417 - 228 0.355 - 238 0.500 - 247 0.376 - 256 0.474 - 316 0.459 - 369 0.374 - 466 0.220 - 502 0.167 - 571 0.525 Yes 584 0.714 Yes 687 0.562 Yes 712 0.331 - 777 0.527 Yes 808 0.207 - 880 0.335 - 918 0.577 Yes 925 0.287 - 975 0.702 Yes 982 0.154 - 999 0.789 Yes 1051 0.322 - 1135 0.290 - 1157 0.086 - 1206 0.061 - 1222 0.048 - 1241 0.038 -

Explain the output -- Processing locus 685: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAACCCCATGTACTTAGCAGGCACACGCCTTAACCACTCGGCCAAAGCGACTATGTTGTTTGCTGAAGGATTAGCTACT ATATAATGTTGTTTTACATAAGTAAAGCGTGGAGCTTACTCGCGTATGTTGCGCGTACGTATGCGTTGTGCTTGTGGTAA AGTGAAAAACACTTTGTGGTTTGTTTTTCGATTGGTCCAGTTCTCTTCAGTTCTTCTTCTTCTCGTCACAGGAAGAAAAA TGTTCTGTTCCTCGCAGCTCTCACAGCCAATGAATGGCCTCAAAGACATAGTTGAGACGAGAAATTTTAAGGCTTGGCTC TTAGATCAGTATGGAGTTCTTCATGATGGCAAAAAACCTTACCCCGGTGCTATCTCAACATTGAAAAATCTTGCAACAGC GGGTGCCAAAATTGTGATCATTAGTAATTCTTCTAGACGTGCTTCAACTACAATGGAAAAGTTGAAAGGCCTTGGCTTTG ATCCTTCTTTCTTCACCGGAGCTATAACTAGTGGTGAACTAACTCATCAATCTTTGCAGAGGAGAGATGATCCTTGGTTT GCTGCACTGGGAAGGAGGTGCATTCACATTACTTGGAATGACCGAGGAGCAATCTCTTTGGAGGGTCTAGATCTAAATGT TGTGGAGAATGTGGAAGAAGCTGACTTTGTTTTAGCGCACGGCACTGAAGCCCTAGGACTTCCTTCAGGCAGTGTATCTC CAAGGACTATTGATGAGCTAGAGAAGATTTTGGAGAAATCTGCAGCTCGAGGACTTCCCATGATCGTAGCTAATCCAGAT TACGTGACTGTTGAAGCAAATGTTTTTCATATCATGCCAGGTACACTAGCTTCCAAGTATGAAGAACTGGGAGGAGAAGT GAAGTCGATGGGAAAACCTCATAAGATGATCTATGAGTCTGCTATAGCAATTGCGGGAGTCAATCCGTCTGAGTCTATCG CAGTAGGAGATTCGCTACATCATGATATAAGGGGAGCAAATGTTTCAGGAATTGAATCAATATTCATCACTGGAGGGATT CATGGTAATGAGCTTGGTCTCACTTCTTTTGATGAAACTGCAAGTCTAGACTCAGTCAAGACCCTTACTGCTAAACACAA TGCATTCCCAACTTATGTATTATCAGCCTTTAAATGGTAGTTTTCGTTTTGTTGTACAAATCATATTATTGCTTCTTGAA CTACATTTTGCCTTCATTTAACACTCAGAGAGAGTAATTTAAAAAGCTCATTTGCTGAG ........N............................................N.......................... .....N.......................................N..............N................... ...............................................................................i .............................i...N.............................................. ..........N...........N..N...................................................... ....................................................i........................... ..................................................................N............. .....................................N......................................N... ........N....................................................................... ............N..............................................i.................... ...................N.............N........................N..................... .......i.................i......N............................................... .....................N.................N........................................ ..............N..................N.............................................. ........................................................... Pos Score Pred ------------------------ 9 0.312 - 54 0.256 - 86 0.252 - 126 0.196 - 141 0.376 - 240 0.526 Yes 270 0.744 Yes 274 0.362 - 331 0.161 - 343 0.133 - 346 0.324 - 453 0.683 Yes 547 0.433 - 598 0.330 - 637 0.174 - 649 0.326 - 733 0.315 - 780 0.560 Yes 820 0.222 - 834 0.424 - 859 0.336 - 888 0.583 Yes 906 0.704 Yes 913 0.191 - 982 0.109 - 1000 0.190 - 1042 0.079 - 1048 0.232 - 1072 0.129 - 1120 0.314 - 1135 0.370 - 1154 0.263 -

Explain the output -- Processing locus 686: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACTTTCCTAAACGGGAATGAGCATGTGATTTTATTTTATTTTACTAGTGTTTAGTGTGACGTCACTTTTGATTGCACTA AAAAAAAGGTGACGTTTTTAACACACATACCTCATGGTGTCTCATGACAATAAAACAATCAGAAGTTCATGCATTTGGTA TAAAAAATTGAAAATTTATTCCGAAGAAAAAGGAGAAAAAATGCATGCGATTTTCTCGAGCTTCTCTCGCAAGGTCGTCG TCAACGTTGGTGCTTCTTCGCAATCGCAGCTAACCAAAATGGTGAAGAAGAAGCCAAACCAGAGTCGCCATCTTCTTCCT TCTCGCCTCTCATCTCCTTCTTCAGTACCCCACTTTGTTCCTTCTGCTGTATCGCGATCGGCGAAAGTTCATGGGTTCTT CGCTAGCAAGTTGGGAAACACCAACCTAAAGCTGAAATTTGGAAATGTTATGGAATCTAGAGCTGGGTTCTTCAGTTCTG AGCTTCCTAGTCATGGATTCGAGTCTGGTGGTTTTACTGGGTTTCAAAAGCGGGGATGGAAGTCTTGGATCAATGGAGCT AATGGTGTAGTTTTTGGACTGGTAATAGCTAATGCTGCTGTATTTACAATGTGGCGAGTTTCAGACAGGAGCTGGATGCT ATCAACGTACAGTTTTACGAGTGGATATATACACACGCTGATAACTTCGGGTTTTAGTCATATTGGTACCAGTCAAATCA TCTTGAACATGATTGGAATCTCCTACTTCGGCTCCAGAATTGCAAGAACCTTGGGACCGCTCTACCTTTTGAAGCTGTAC TTTGCTGGAGCACTTGGTGGCTCTGTTTGCTTCCTGAGTTATCACGCCCTCTTGGCTACACTCAAGGGTGAAGGAGTTGT CATTAAGGATCATCAATCAACAGCCCCCATTTCGCAGCTGCTGGGTGCTGATGGATCTATGTTTGCCATCGCGCTTCTCG ATATGTTCATCTACCCAAAAGTTACAACATACTTTGCGTTGATGCTCCGAGTGCATGTAATGTTTGGGGGACCGAACCAT ATCGCATCGAGTTCGGGTCAGTTGGGAGGAGTTGTGGTTGCTGCCATGGCGTGGGCACGGATAAAGAAAGGTCGATTTTG ATACTGAACTGATTTGCTGCAAAAGATTCAGTGGACCTGATCATCATCATGCAAGTTATGTTAAACTTCGTGACAAATGA GATTTTTGTCAACATTTTTT .................N.....N........................................................ .................................N.........N........................N........... ........................................i...N................................... ......................................i......................................... ......................................................................N......... ............................................i....N.............................. ............N..........................................N................N....... ................................................................................ ........N....................................................................... ................................................................................ ..................................................N.......N..................... ..N......................................i............N....N.................... .............................................N.................................. ................................................N........N..................N... .................... Pos Score Pred ------------------------ 18 0.433 - 24 0.180 - 114 0.379 - 124 0.308 - 149 0.199 - 201 0.916 Yes 205 0.234 - 279 0.563 Yes 391 0.339 - 445 0.639 Yes 450 0.189 - 493 0.221 - 536 0.281 - 553 0.245 - 562 0.349 - 592 0.118 - 609 0.372 - 636 0.279 - 729 0.486 - 931 0.146 - 939 0.487 - 963 0.471 - 1002 0.701 Yes 1015 0.403 - 1020 0.309 - 1086 0.199 - 1169 0.082 - 1178 0.050 - 1197 0.044 -

Explain the output -- Processing locus 687: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATGATTCTCTGCAGTGGCCAGTAGATTATTTATTTGGTAGCCTCATATTAAATTATACGTTTTCGAAAAATGAGAAATT CCCGGTTTTACTTCGGTTTGATTTGGTTACTATTATAGTCGAAACCGGAATATTTTCAGAGAAAAAAAACTACAACTATT GGGCCTATTTGTTGAGCCCATAAATAATGTTATGAAGCCCGATTTGAGACAGATCGTTATCGTTTCGGGGTCACAGGGAC TTTCACTCTTTCTCTCTCTCTGCAACAAAGAAGAAATGGAGGTAGCAGCAGCGACTGCGACGAGCTTCACAACGCTTCGA GCTCGTACGTCAGCGATTATCCCGTCTTCTACACGTAATCTGAGATCTAAACCGAGATTTTCTTCATCTTCATCTCTCAG AGCTTCTCTTTCGAATGGCTTTCTTTCGCCGTATACCGGAGGAAGCATCTCTAGTGACTTATGCGGCGCTAAGCTTCGTG CGGAATCGCTTAATCCGTTAAATTTTTCCAGTTCCAAGCCTAAACGCGGAGTTGTCACTATGGTTATACCTTTCTCAAAG GGAAGTGCACACGAACAACCTCCTCCTGATTTGGCATCATATTTGTTCAAGAACCGAATTGTATATTTGGGAATGTCTCT CGTACCTTCAGTTACTGAGTTGATACTTGCGGAGTTTCTTTACCTTCAGTATGAAGACGAGGAAAAGCCTATTTACCTTT ACATAAACTCGACTGGGACAACCAAGAATGGTGAAAAGTTGGGCTATGATACTGAGGCTTTTGCAATCTATGATGTCATG GGGTATGTCAAACCACCAATCTTTACTCTTTGCGTCGGGAATGCTTGGGGTGAAGCTGCTTTGCTTCTGACTGCTGGTGC AAAAGGAAATCGATCTGCGTTGCCCTCATCAACTATTATGATAAAGCAGCCCATTGCTCGATTTCAAGGCCAAGCAACTG ATGTTGAAATTGCAAGGAAAGAAATCAAGCACATAAAGACAGAAATGGTCAAGCTGTATTCAAAGCATATTGGTAAATCC CCGGAGCAGATTGAAGCTGACATGAAACGCCCGAAATATTTTAGTCCCACTGAGGCTGTTGAATATGGGATCATTGATAA GGTGGTTTACAATGAAAGGGGCAGCCAAGACAGAGGAGTTGTGTCTGACCTTAAAAAGGCACAACTCATTTGAATGTCAG AACTGTCTTCCGAAATCCCATGATTAACAGGTAAAAGCTAAAATTTGCTCACTGTCATTCTACATATGGATTATAATGAT GATGAATTTGCT ................................................................................ ..........................N....i................................................ ...................................i............................................ ................................................................................ ..............i.............................................N................... ...........................................................N.................... ........................................................................i....... ..................................................N............................. ...........................N.................N.......................N..N....i.. ....N...................................N....................................... .....................................N.......................................... N...........................................i................................... .....................i..........................................N............... ...........N.............................................................N...... ...................N.............................................N.........N..N. Pos Score Pred ------------------------ 2 0.111 - 71 0.255 - 187 0.485 - 192 0.665 Yes 276 0.795 Yes 415 0.663 Yes 461 0.371 - 540 0.413 - 633 0.644 Yes 691 0.184 - 748 0.313 - 766 0.097 - 790 0.106 - 793 0.320 - 798 0.818 Yes 805 0.183 - 841 0.363 - 918 0.283 - 961 0.179 - 1005 0.644 Yes 1062 0.616 Yes 1105 0.436 - 1132 0.408 - 1194 0.310 - 1220 0.026 - 1266 0.090 - 1276 0.048 - 1279 0.068 - 1282 0.031 -

Explain the output -- Processing locus 688: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATAGGAGATTAGTATGTTAAATGCTTGGTATTTTTGTGTCTTAATTGTATGCTTTATCAGTTAAGTACATTTTTGAGTT TGTTATTCTGACAGGAGTTAGATGCGTACTTCTTCTGACAGGAACAGGATCGCTTCTACAGATATTTCACCCATGTCAAG TTTAACAGTGAGAAGGGTATCGCGCGAAGATATACAACTGGTTCAGAATCTAATTGAACGATGCCTCCAGCTTTACATGA ACCAGAAAGAAGTTGTTGACACTCTTCTAGAACAGGCTAAGATCGAACCTGGTTTTACAGAACTAGTTTGGCAGAAGCTT GAAGAAGAGAACCGCGAATTTTTCAAGGCATATTATCTGAGGCTCATGGTGAAGCACCAGATAATGGAATATAACGAACT GCTTGAGCAGCAGATAAACCACATGCGCCAGATGCATCCAACTGCAGGGGCTTCTGTTCGAAACAGGAATGGTTCTCATG TTCCACCAATGAATCAGCAACAATTACTCTATGAACGCAAGGAACCAGATCAATCCTCTCCTAATCTGTCAAGTCCATAC CTCAATGGAGGCTCAGCAATTAACACAAATATACCTTCTTATGTGGACTTTTCATCCCATTCTAGAAGAGTTGATCCTTC ACCAAACTCGCTCTCCTTGCAGGCCACAAATATGCCTTTGATGCAAGGAATGATCAAGTCTGAGACTGCATATCAAAACT GTGCTCCATACATGTATGGTGGTGAAGCACAGTCCACAGTTGGAGATGTCACCATCGCATCTTTCAGCAATGATTCCAGC AACCAATCCCTGAATGATCCTCTTGTCGATCCAGATGCTCCTACATTTGGCTCGTTAGGACAAATTCCTCAGAACTTCAG CCTCTCTGATCTGACAGCTGATTTTTCCCAGAGTTCAGATATTCTGGAGAGCTACGAGGGATCACCGTTCCTATTGGCTG ATGCTGAAAATTTCCTGGACTCTAGCGAAAGGGTAGAACATCAAGGAGACCACGAAAGATTGAGGACCATATCATCAGGC TTCAGTTACGAAAACTTCCGAAGCAATTAGGTTTATTACACATGGAACTTCGTAGTCATGCTTTTACGTCTGCAACTACT TGCAGGATTTAATCCCATGATCAGTGTACATAGATATTCTTACCTTTCG ..............N......N...........................N.............................. .....................N..................................................N....... ............................................................i...............i... ................................................................................ .............................................N.................i................ ......................i........N....................................N........N.. ........N.....................N................................................. ....i...................................N....................................... ...............................i........N........i.............................. ...........i...N.............................N.......................N.......... .............N....................N............................................. ................................................................................ N............................................................................... .........................................N...............N...................... ................N................................ Pos Score Pred ------------------------ 15 0.340 - 22 0.343 - 50 0.142 - 102 0.197 - 153 0.394 - 221 0.541 Yes 237 0.610 Yes 366 0.299 - 384 0.629 Yes 423 0.660 Yes 432 0.466 - 469 0.335 - 478 0.491 - 489 0.462 - 511 0.253 - 565 0.564 Yes 601 0.323 - 672 0.848 Yes 681 0.460 - 690 0.759 Yes 732 0.607 Yes 736 0.213 - 766 0.165 - 790 0.236 - 814 0.093 - 835 0.213 - 961 0.440 - 1082 0.122 - 1098 0.059 - 1137 0.049 -

Explain the output -- Processing locus 689: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATTAATATCAACAAACATTTTTAAAAAGACCCCTCATCACTATTATATTTCAAAATCAGGCGAGTACAAAACAAATAT GAGCATTTTAATCACATGTAAATCTTCGAACAAAACCAATCCAATTGGACCCACCTGCACCTTTTCATTATCCAACATTT ATATATTAATCGAAACACTCACATGGATGGATTGGATTGTCTCAACTCTCAACCCAAAATCAAACAAAATATCCAGCAAA ACAAAAACTTTCTCTCTCACTGTCTCACACACACAATCTCACCTTCGTCAAGCTTTTGCGAGATGGGTGCGATAAACCAA GGAATAAGCTTGTTTGATGAATCACAAACCGTCATAAACCCTATTAATACCAACCATCTAGGTTTCTTCTTCTCTTTCCC TAGTCACAGCACCTTATCTTCATCATCTTCGTCGTCTTCGTCTTCTCCTTCTTCTCTTGTGTCTCCATTTCTTGGTCATA ACTCCCTAAACTCCTTCCTTCATAATAACCCGTCTTCATTCATAAGTCATCCTCAAGATTCCATCAATCTCATGACCAAT CTCCCCGAAACCCTAATCTCGTCTTTGTCCTCATCAAAGCAAAGGGACGATCATGATGGTTTTCTTAATCTCGATCATCA TCGTCTTACCGGTAGTATTTCATCCCAAAGACCCCTGTCAAATCCATGGGCATGGAGTTGTCAAGCGGGATACGGAAGCA GCCAGAAAAACAACCATGGAAGCGAGATTGATGTTGATGATAATGATGATGAGGTTGGCGATGGTGGTGGCATTAATGAT GATGATAATGGTCGTCATCATCATCATGATACTCCCAGTCGTCATGATAAACATAACACAGCGTCATTAGGCGTAGTTTC TTCTCTGAAGATGAAGAAGCTTAAGACAAGAAGAAAAGTGAGGGAACCTCGGTTTTGCTTTAAGACACTTAGCGAGGTTG ATGTCTTAGATGATGGATATAGATGGAGAAAGTATGGCCAGAAAGTTGTCAAAAACACCCAACATCCCAGGAGCTATTAC AGATGCACACAAGACAAGTGTAGAGTGAAGAAGAGAGTGGAGAGATTAGCAGATGACCCAAGAATGGTAATCACTACTTA CGAAGGAAGACACCTTCACTCTCCTTCTAATCATCTCGACGACGACTCTCTCTCCACCTCTCACCTGCACCCTCCTCTCT CCAACTTCTTCTGGTGATTTCAATTTGTATTTCCATTGAATCAACCAATATCAAAAATCAATCAACCTTGCCTTGCCTCA AACTAATTATCGTATTATATATTAATCATCAACACC ..............................................................................N. ...............N................................................................ ......................i...N..................................................... ..............................................................N................. ................N............................................................... ................................................................................ .......................................................................i........ ....................................................i..i........................ .............................................N.....N............................ ...............N..............N.....N.....N..N..N...........N..............N..N. ..........i..................................................................... i........N..N.........i..........i.............................................. ..N.................................................N..........i................ ................................................................................ ................................................................................ .................................... Pos Score Pred ------------------------ 79 0.410 - 96 0.275 - 183 0.679 Yes 187 0.307 - 303 0.480 - 337 0.282 - 552 0.619 Yes 613 0.643 Yes 616 0.796 Yes 686 0.141 - 692 0.324 - 736 0.418 - 751 0.183 - 757 0.183 - 763 0.154 - 766 0.326 - 769 0.223 - 781 0.259 - 796 0.135 - 799 0.086 - 802 0.097 - 808 0.195 - 826 0.131 - 844 0.074 - 891 0.605 Yes 961 0.508 Yes 970 0.368 - 973 0.435 - 983 0.503 Yes 994 0.602 Yes 1043 0.264 - 1093 0.476 - 1104 0.669 Yes

Explain the output -- Processing locus 690: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATTGACTTTTTCAAAAGTCAACACAATAAATTTTAAAAGTTTAATGACTTAACGGGTTCACATGGGAAACGAAAACAC CCTAAACCACAAACAATCTAATCTTATTTCCTTCTTTATATAAACCGCTGTTTCCCAAAAGGCTTGTTCTCGTCATATGT ACTTGTACACCAACCCACCAAAAGAGATAAAAGAGGAAACAAAAACTCGAAAAGAGAGAGATATATGGGTGAGGTGGCTT ATATGGACGAAGGAGACCTAGAAGCAATAGTCAGAGGCTACTCCGGCTCCGGAGACGCGTTTTCCGGCGAAAGTTCCGGT ACGTTTTCACCTTCGTTTTGCCTACCGATGGAGACGTCTAGTTTCTACGAACCGGAGATGGAGACAAGTGGCTTAGATGA GCTCGGTGAACTTTACAAACCCTTTTACCCTTTCTCCACACAAACGATCCTCACAAGCTCGGTCTCTCTCCCTGAAGATT CAAAACCTTTCCGAGATGACAAGAAACAACGATCACATGGTTGTCTTTTATCCAACGGATCAAGAGCTGATCATATCCGA ATTTCAGAATCCAAATCAAAGAAAAGCAAGAAGAATCAACAGAAGAGAGTTGTTGAGCAAGTGAAAGAAGAGAATCTGTT GTCGGACGCATGGGCGTGGCGTAAATACGGGCAGAAACCCATCAAAGGATCTCCATACCCAAGGAGTTATTACAGATGCA GTAGCTCAAAAGGGTGTTTGGCAAGAAAACAAGTCGAAAGAAATCCTCAAAACCCGGAGAAATTCACCATAACATACACT AATGAGCACAATCATGAACTACCAACCCGGAGAAACTCATTAGCCGGTTCGACTCGAGCAAAAACTTCCCAACCCAAACC AACCTTAACCAAAAAATCCGAAAAAGAAGTTGTTTCTTCCCCTACAAGTAATCCTATGATCCCATCCGCTGATGAATCTT CTGTTGCGGTTCAAGAAATGAGCGTTGCGGAAACGAGTACGCACCAAGCGGCTGGAGCAATCGAGGGCCGCCGCTTGAGT AACGGTTTACCATCGGATTTGATGTCCGGGAGCGGAACTTTTCCAAGTTTTACCGGTGACTTCGATGAACTATTGAATAG CCAAGAGTTCTTCAGTGGGTATTTATGGAATTACTAGAGAGCATTAGGTGTATGTATATATATATATATATATATATATA TATATATTCAAAATTATATTATAGTTTTAGCCATTGAGGAAAATGTATATTTATGT .............................................N.................N................ ............................................................................i... ................................................................i............... ..N............................................................................. ...........................N.............................N..................i... ................................................................................ ...............N....................i........................................... ................................................................................ .........N.................................................................N.... ................................................................................ .......................................................i...............N........ .................i.............................................................. .....................i..........................................N............... ........................N..........................N............................ ..........................................N.........N... Pos Score Pred ------------------------ 46 0.398 - 64 0.342 - 157 0.602 Yes 225 0.812 Yes 243 0.446 - 348 0.441 - 378 0.350 - 397 0.667 Yes 496 0.454 - 517 0.508 Yes 650 0.460 - 716 0.395 - 802 0.557 Yes 814 0.300 - 936 0.794 Yes 952 0.387 - 978 0.801 Yes 1062 0.716 Yes 1105 0.235 - 1145 0.135 - 1172 0.053 - 1243 0.053 - 1253 0.051 -

Explain the output -- Processing locus 691: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAACTGGCCAAGTCGGAGTCGTGTGAGAATAATGGGGACGGATAGGGTACGTGGCACTATTTTATAGGTTAGAAAAAC GACCAGCCCGCGGTATTTTAGATCCAGTTGACGATAATGAACCAAGACGAGAATCTGTCTAGGTTCCTTCTTCTTCTTCC ACAATATAATTATATCTTTCTTCACACACCCCTAGAGAGAGAAAGGAAGGTGTTACCGGATTTAGGAGAGATAAAGAAGG TTTCTTCATTTAGAAACAGAGACACGAAGTTTGCGATGATGATGATGATTCAGAGAAGAGGAGGTGAGAGACAAGACTCG TCGGCGGCGGCGTACAACGTTGTCCATAAGTTGCCTCACGGAGACAGTCCTTATGTCCGAGCGAAGCATGTTCAGTTGGT GGAGAAAGATGCAGAAGCAGCGATAGAGTTGTTTTGGATAGCGATTAAAGCGAGAGATAGAGTTGATAGTGCTCTTAAAG ACATGGCCTTGTTAATGAAACAACAGAACAGAGCTGAAGAAGCCATTGACGCTATTCAATCCTTTAGAGATCTTTGTTCA AGACAAGCTCAAGAGTCATTAGACAATGTCCTCATCGATCTATATAAGAAATGTGGGAGAATAGAAGAGCAAGTTGAGTT ATTGAAGCAAAAGCTTTGGATGATATATCAAGGAGAAGCATTCAATGGCAAACCAACAAAGACTGCGAGATCTCATGGCA AGAAGTTTCAGGTCACCGTCGAGAAGGAAACCTCTAGGATCTTGGGGAACTTGGGATGGGCTTATATGCAACTAATGGAC TATACAGCAGCTGAAGCTGTGTATCGGAAAGCTCAATTGATAGAGCCAGATGCAAACAAGGCTTGCAATTTATGCACATG TCTCATCAAGCAAGGGAAGCACGATGAGGCGAGGTCGATTCTCTTTCGTGATGTACTAATGGAGAACAAAGAAGGGTCTG GTGATCCGAGGTTGATGGCTCGGGTTCAAGAGCTATTGAGTGAGCTAAAGCCACAGGAAGAAGAAGCAGCGGCTTCTGTG TCTGTGGAATGTGAAGTTGGTATAGATGAGATAGCGGTTGTCGAAGGACTTGATGAGTTTGTGAAGGAATGGAGGAGGCC TTATAGGACAAGAAGACTTCCGATTTTCGAAGAGATCTTACCACTGAGAGATCAATTGGCTTGTTGATGATTCTCTGTGT GTTTGGATTGATGTTTGAATACTGAATAGGAACCTTTTTGGGTAGAAGAATGTAGTGAGTGAATAGAGTTTACCTCTTGT AAGCTT .................................N.............................................. ....................................i........................................... ................................................................................ ...................................N..N..i..N................................... ....................................................N..............N............ ........N....................................................................... ..N...........N................................................................. .........................N........................i............................. ...................i........................N.............................N..... .......................................................N.........N........i..... .................................................N.....................N.....i.. .......................N..........................N.......i..................... ..............i................................................................. ........N................N..........................N...............N........... ..................................................................N............. ..........N......................................N.............................. ...... Pos Score Pred ------------------------ 34 0.346 - 117 0.556 Yes 276 0.344 - 279 0.425 - 282 0.534 Yes 285 0.485 - 373 0.207 - 388 0.199 - 409 0.368 - 483 0.342 - 495 0.200 - 586 0.252 - 611 0.830 Yes 660 0.640 Yes 685 0.456 - 715 0.280 - 776 0.429 - 786 0.250 - 795 0.693 Yes 850 0.263 - 872 0.188 - 878 0.583 Yes 904 0.431 - 931 0.257 - 939 0.692 Yes 975 0.734 Yes 1049 0.473 - 1066 0.145 - 1093 0.180 - 1109 0.483 - 1187 0.071 - 1211 0.061 - 1250 0.082 -

Explain the output -- Processing locus 692: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTCCAAATTAGAACTCGAACCGAAAATAACATTGTAATTAAATTATACCGAATCTAACCGAAATATTCGAACCGGATA ACGGAAGTAAAATGATTTTAATGGTACGGCCGTAAAATGTTTGGGCCAATACGGCCCATTCGTTATGACTTTTTTTATTA TTATTACAGAATCGTTTCCATTAAACCCTTAAAAGTTTCAGAATCGAACTCGTCTATTCTCCGCCGGTGTGCCGTCGCCG ATATGCTTCGTCGTGTCTTTTACAAGCGTGCACGTGATGATTTATCTTACTGTCATCGTCTGTTGCACATTCCGTATAAC GAACCAGAGCCTGACTCAAAGGCAATTTCTGAAATCGTTGGTATAGTTTCAAAGAAAGAAGAAGCTGTAGTAGTTACGGA ACCAACTCATCATTGGCGTAAGAATTGGGAAGATTTGAGAAAGAATAGGTTAACAGCTAGTAATTTCGCGCGTGCTATCG GATTTTCGCCTGACGGTAGACGAAATCTTTGGTTAGAGAAAATCGGGGCAGCGAAACCGTTTGCTGGAAACCGAGCTACG TTTTGGGACATTGAGAATGAGGTCGAAGCACTCGAGAGGTACAACGAGTTAACGGGAAATGAAATCCTCATACCTGAGTT TGTTGTTTATAAGAATGGTGAAAGCCCTGAAGAAAACTGGTTAGGAGCTTCTCCTGATGGAGTGATCAATGTAGTTAAGG ACGGTGTTACTTCGTGTGGTGTGTTAGAAGTGAAATGCCCGTTTGATAACCGGGATAACTCGAAAGTTTATCCGTGGAAG AAGGTTCCTTATAATTGTGTTCCACAACTTCAGGGTTTAATGGAGATTGTGGATACAGATTGGTTGGATTTGTATTGTTG GACTCGCAATGGAAGCAGTCTCTTTAGAGTTTGGCGTGATACCGCGTTTTGGGAGGATATGAAACCGGCACTGTTTGATT TTTGGCAGAATCATGTTTTACCTGCAAGAGAGATATACAATAACTTTGATATAAAGGATCCTCAAGTGAAGCTGAGAGAG TTTAAACCGAAGCATTGGCATGAAGATTGCAAGAAGATTATGCGTGGAGCTGAGAGAATCAGCGCAAACGCAAACCGTTT GTTCTATGAGATCGATGGGAATCTTGTAGACTGAGGTTTTTGTAACTGATTATGTGAAATTTGCAAGCAAGCAAGCCAGG TTCCCTTATTTCATTTGAATGTTATTTGCTTAGTGTGGAATGTAGAAAACACT ................................................................................ ...........N........N...............N...........................N............... ................................................................................ ..N.................................N........................................... ................................................................................ ................................................................................ ................................................................................ ................N.........................................N..................... ..............N.........................................N...........N........... ..................................N............................................. .......................................N........................................ ........N.................................................i..................... ............N................................................................... ...................N...................i........................................ .....N........N....................................N............................ ..................N....................N............. Pos Score Pred ------------------------ 92 0.192 - 101 0.420 - 117 0.387 - 145 0.405 - 243 0.458 - 277 0.197 - 577 0.471 - 619 0.271 - 655 0.472 - 697 0.217 - 709 0.266 - 755 0.431 - 840 0.486 - 889 0.479 - 939 0.628 Yes 973 0.296 - 1060 0.237 - 1080 0.673 Yes 1126 0.114 - 1135 0.100 - 1172 0.122 - 1219 0.029 - 1240 0.067 -

Explain the output -- Processing locus 693: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGATCTGGGGGTTAGCTCGTAATCTGGCTCTAATCATTTTATAGTTTTATTATCTCTACAATGTCACTCCTGGGAGGCT TGTACCGGAATCCCTTGGTAAAACTTGGGGCATTCTACCGGTATAGCCAAATCCACAGGACTAGTCATTTGCTTATGTTC ATCAAACATGCATAGTTTGAATGAAACAGTAATTCCTGATGTTGATTACATGCAGTCTGATAGAGGGCATATGCATGCTG CTGCCTCTGATTCCAGTGATCGATCAAAGGATAAGTTGGATCAAAAGACCCTTCGTAGGCTTGCTCAAAATCGTGAGGCA GCAAGAAAAAGCAGATTGAGGAAGAAGGCGTATGTTCAGCAGCTGGAGAATAGTCGATTAAAGCTGACTCAACTTGAGCA GGAGCTGCAAAGAGCAAGACAGCAGGGAGTTTTCATCTCAAGTTCAGGAGACCAAGCTCATTCTACTGGTGGCAATGGGG CTTTGGCATTTGATGCAGAACACTCACGATGGCTTGAAGAAAAGAACAGGCAAATGAACGAGCTGAGATCTGCCCTGAAT GCTCATGCAGGTGATACTGAGCTCCGGATAATTGTGGATGGAGTGATGGCTCACTATGAGGAGCTTTTCAGGATTAAGAG CAATGCAGCTAAGAATGATGTCTTCCACTTGTTATCTGGAATGTGGAAAACACCAGCTGAGCGATGTTTCTTGTGGCTTG GCGGGTTCCGCTCATCCGAACTTCTCAAGCTTCTTGCGAATCAGCTAGAGCCCATGACAGAACGACAGGTAATGGGCATC AATAGCTTGCAGCAGACGTCGCAGCAGGCAGAAGATGCTTTATCTCAAGGGATGGAGAGTTTACAGCAATCCCTAGCTGA TACTTTATCCAGTGGAACTCTTGGTTCCAGTTCATCGGATAATGTCGCGAGCTACATGGGTCAGATGGCCATGGCAATGG GGCAGTTAGGCACCCTCGAAGGATTCATACGCCAGGCTGATAACTTGAGGCTGCAAACACTACAACAGATGCTTCGAGTA TTAACAACACGTCAGTCAGCTCGTGCTCTTCTTGCTATACACGATTATTCATCTCGATTACGTGCTCTTAGTTCCTTGTG GCTTGCCCGGCCAAGAGAGTGAAACAGAAGAATAGTGCTCTACTCAGCAGTCAGCCCTATACATAATCTTATGACTCAGA TCGGCAGGGATACTCCTTCCAAGAGATCGATCACAAAGCAA .............................................................N.................. ..........................................................................N..... .......i............N.................N..........i....................i...N..... ................................................................................ ...............................N................................................ ..........................................................................N..... ............N...............i........................i........................N. ....N................................N.......i.........N........................ ..N...........N..i......................i......................i................ .....................................................N.................i........ ..................................N................i............................ .........................................i.............N........i.....N.....N... ....................................................................N........... ................................................................................ ......................................................................N......... ......................................... Pos Score Pred ------------------------ 62 0.324 - 155 0.189 - 168 0.894 Yes 181 0.332 - 199 0.457 - 210 0.612 Yes 231 0.691 Yes 235 0.166 - 352 0.288 - 475 0.218 - 493 0.121 - 509 0.683 Yes 534 0.614 Yes 559 0.148 - 565 0.218 - 598 0.219 - 606 0.649 Yes 616 0.194 - 643 0.414 - 655 0.440 - 658 0.512 Yes 681 0.513 Yes 704 0.631 Yes 774 0.335 - 792 0.530 Yes 835 0.355 - 852 0.694 Yes 922 0.558 Yes 936 0.374 - 945 0.609 Yes 951 0.200 - 957 0.484 - 1029 0.338 - 1191 0.133 -

Explain the output -- Processing locus 694: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTGATAATCTCAAACGATTACAGTATAACATAAAGCATATTAACAATTTAATAATTAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAATTTAATAATTAATGCTAGTTTTGTTTCTGGCTAAGCACACGCGCATAAGAGAACACGCTTTAGAATT AACAAGGAGTGTATTACACGCGCTTGGTTTTCGCGTAAAAGCCTTGTTTAGCGTATTATTATTTCATAAGTTCGATTTCC TAATTCCTAATCCGTTTCCTTTTTATTCCGATCAAAGCAGCGAAAAAAGCATCGGAGATATGTCGTCTAAGGACAAGAAA CTATCGAAGCCAACAAGTGGTCGTACTGGTGGTATCCGTACACTTTCCGATCTGAACCGAAGATCAGAACCTGATTCCGA TAGTGACTCCGATGGACCTCAAGAATACTTCACCGGTGGCGAAAAAAGTGGTATGCTTGTTCAAGATCCTACAAAAGAAC CAAAACACGATGATGTTGATGAGATATTTAATCAAGCAAGACAGCTAGGAGCTGTGGAAGGACCTCTTGAGCATCCTTCG AGTTCTAGAAGCTTTACAGGAACAGGAAGATTGCTTTCTGGAGAAAGTGTGCCAACGGCTCTTCAACAGCCTGAGCCTGT GATTCACAATATTATTTTCTGGTCTAATGGATTCACTGTAGATGATGGACCTTTGAGGAAGTTGGATGATCCAGAGAATG CTTCTTTTCTTGATAGCATTCGAAAGTCTGAATGTCCGAAAGAGCTTGAGCCTGTTGATAAAAGAGCTCCTGTACATGTT AATCTTATGAGAAGAGATGAGAAGTGCCCTGAGAAAGAAAAGCTTAAGGTTTCATTTCAAGGAGTTGGAAGGACTCTAGG TGGTGCAAGCTCATCAACAGCTTCAAGCCAAAGTAACCTAACTGATGTAGCAGCGGTTCAATCACCGTTACAGAGCCTTG TAGTAGATGAAACTCTTCCATCCACATCGATCCAGCTTAGACTCGCGGATGGGACACGCATGGTAGCAAAGTTCAACAAC CATCACACGGTTAACGACATTCGTGGTTTCATTGAGTTTTCTCGACCAGGGAATCCAAACAACTATACCCTTCAGGTCAT GGGGTTCCCACCAAAGCCACTTACTGACCCTTCTCAGACCATTGAACAAGCCGGTCTAGCAAGTTCTGTAGTCATTCAGA AATTCTAGAAAAGTTTCGTTTCTCTTTTTTTGTACTAGAGAGAGATTTCAACAACTTGGAATTGTACTGAAAATATTCAA TCGGCTTATTGTTGTGGCAAGAGAAAT ................................................................................ ........................N....................................................... ................................................................................ ...........................................................i.................... ................................................................................ ...........N........................................i........................... .........N..N.....N............................................................. ................................................................................ ..........................N..............N..N....................N...........N.. ...............................i...........................................N.... ......i.........i............................................................... ............................................N................................... ......N.........................................N..........N.................... ..............................................................................N. ................................................................................ ................................................................................ ........................... Pos Score Pred ------------------------ 105 0.249 - 300 0.831 Yes 412 0.188 - 453 0.736 Yes 490 0.460 - 493 0.219 - 499 0.192 - 667 0.208 - 682 0.234 - 685 0.320 - 706 0.111 - 718 0.206 - 752 0.572 Yes 796 0.191 - 807 0.507 Yes 817 0.616 Yes 925 0.346 - 967 0.150 - 1009 0.397 - 1020 0.424 - 1119 0.296 -

Explain the output -- Processing locus 695: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGCGATTGTTTTGAAAGTGTTATAATTGATCTTGCTTCTGCTGTGTTGTATCTTGTACTTACATGTGATGTATGAGTAT CGAAGTTGTTGTGACAGATAGCATATGAGCCTTTACATTGTTTCAACTATTAGCTGTGAGATATTTGTGAACCTTGTAGT TTATGATCTCTGCTTTTCTTCCATGAGCTGTGTTTTACAGGCTATTTTGCTGTGTGTGTTGTTCCACACAGATGATCTCA TTCAACAATTTTGCAATTTTACTTCTATTTGCAGGGACAATCATTTCGGAAACATGCCAGGTCAGAAGATTGAAACGGGT CATGAGGACATTGTCCATGATGTGCAGATGGATTACTATGGAAAGCGAATTGCAACTGCATCATCTGACTGCACCATCAA GATAACCGGTGTCAGCAACAACGGTGGATCGCAGCAACTAGCCACATTAACCGGCCACCGTGGTCCTGTCTGGGAAGTCG CGTGGGCACACCCAAAGTATGGATCAATCCTTGCTTCATGCTCCTATGATGGTCAAGTCATACTCTGGAAAGAAGGCAAC CAGAACCAATGGACCCAAGATCATGTTTTCACAGACCACAAATCTTCAGTCAACTCCATTGCATGGGCTCCTCATGATAT CGGACTATCCTTGGCCTGCGGGTCATCTGATGGCAACATTTCGGTTTTCACAGCCCGTGCTGACGGTGGCTGGGACACAT CAAGGATTGACCAAGCACATCCGGTTGGAGTCACTTCAGTCTCATGGGCCCCAGCCACAGCGCCTGGTGCTCTTGTGAGC TCTGGTCTTCTCGATCCGGTTTACAAGCTAGCTTCTGGTGGATGTGATAATACCGTGAAAGTGTGGAAGCTCGCTAACGG GTCATGGAAGATGGATTGCTTTCCAGCTCTTCAAAAGCACACTGATTGGGTCCGTGATGTGGCTTGGGCACCAAACTTGG GTCTCCCCAAGTCCACCATAGCCAGTGGTTCCCAAGATGGGAAAGTTATCATATGGACAGTGGGAAAAGAAGGTGAGCAA TGGGAAGGTAAGGTTCTCAAGGACTTTATGACTCCTGTGTGGCGGGTCTCGTGGTCGTTGACCGGTAACTTATTGGCCGT ATCCGATGGGAACAACAACGTGACTGTGTGGAAAGAGGCTGTTGATGGAGAGTGGGAACAAGTTACTGCTGTTGAGCCTT AGGTTTTTGATGCTTTGCTATCTCTCTGCTCTTCCTTTAAGGAACTGAATCAAACATTTTTTCTTTACTCTTTTCTTAGT CCGGTTTGGTGGTCTTTTTCA N..............................................................N....N...N....... ........................N....................................................... ..N...................N................................................N........ .....................................................i.......................... ................................................................................ ..................i..................N.......N..N............................... ........N.............N.......................................N..........N...... .............................i.................................................. ...........................................N.................................... .........................................N...................................... ...i......i.............................................N....................... ....................................N...............i..........................i ...........................i.................................................... .....N......................................N................................... .........N...................................................................... ..................... Pos Score Pred ------------------------ 1 0.218 - 64 0.269 - 69 0.262 - 73 0.256 - 105 0.336 - 163 0.139 - 183 0.468 - 232 0.215 - 294 0.864 Yes 322 0.356 - 337 0.582 Yes 340 0.192 - 348 0.432 - 358 0.452 - 499 0.855 Yes 518 0.187 - 526 0.269 - 529 0.326 - 569 0.371 - 583 0.408 - 623 0.282 - 634 0.364 - 670 0.724 Yes 764 0.368 - 842 0.329 - 884 0.603 Yes 891 0.786 Yes 937 0.238 - 997 0.330 - 1013 0.612 Yes 1040 0.772 Yes 1068 0.653 Yes 1126 0.181 - 1165 0.299 - 1210 0.067 -

Explain the output -- Processing locus 696: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGATGGGATTTGTAAAGATGGAAACTCTGTAATCCATTTGCTGGTTAAGAAATCGGTGGAAGACACGGTGAAAAGAGA AGAAGACACTGCTACTGGTAAAGACTCCTTGTTAGAGCCGTTGTTCTTAACCCCGATGTGAAATTACCTGAGGTTCTCGA GGATATGATTGACCGGACGGTTGATGGGTTGAACAAAGGTAGTCCACCAGTGAGATCAGCTGAAGGGACAGGAGGGACAT ATCTGATGCAAGATTCTTCAGGTCTCAACTATGTATCTGTGTTCAAGCCTATGGATGAAGAACCAATGGCTGTGAACAAT CCTCAGCAGCTTCCTGTGTCATCAGACGGTCAGGGATTGAAGAGAGGAACTAGAGTAGGAGAAGGGGCAACAAGAGAAGT TGCAGCTTACTTGTTAGATCATCCAAAGAGCGGACTAAGATCTGTGTCTAAAGAAGTTATGGGTTTTGCCGGTGTGCCAC CAACTGCTATGGTCAGAAGCTCACACAAAGTGTATAACTACCCAAATGGGTTCAGCAGCTGTGCTACAAAAGATGCCAAA GTTGGTTCTTTGCAAATGTTCATGAAGAACAATGGAAGCTGCGAAGACATCGGCCCTGGAGCGTTTCCTGTTGAGGAAGT GCACAAAATCTGTGTCTTTGACATAAGAATGGCAAATGCAGATCGACATGCTGGAAACATATTGACCGGGAAAAGCGAAG AAGGAAAAACTCTTCTTATTCCCATCGATCATGGTTATTGTTTGCCCGAGAATTTTGAAGATTGCACATTTGAGTGGCTC TACTGGCCGCAAGCGAAACTCCCGTTTTCTGCAGACACCATTGACTACATAAACTCTCTAGACTCCGAACAAGACATTGC GCTTCTGCAACTCCATGGCTGGAATGTTCCTGAAGCTGTCTCACGCACTCTACGTATCTCCACGATGCTGCTGAAGAAAG GCGTTGAGAGAAACCTGACGCCATATCAAATAGGAAGCGTAATGTGCAGAGAAACGGTGAACAAAGACTCAGCGATTGAA GAGATAGTGAGAGAAGCTCATAACTCGGTGTTGCCTGCGTCAAGCGAGGCTACGTTTCTTGAAGCAGTATCTGTGGCCAT GGATCGTCGTCTGGACGAGCTAACTAAGTAACCAGTCTTAAGTACATAATAAAGTTTGGTTTTATGGTATGTATATGTAT ATGATGTAATGTATCTATTATGTTTGTTTTCGTTGAGAAAAACGTTGATA ....N..............i............................................................ .......................................................N........................ ....N..................N........................................................ .....i........................N...................i...N..........i.............. ................................................................................ ..........................................................i..................... ........i....................................N..........................N....... ...............i.....i.........i................................................ ............................i......N...........N................................ ..............................N................................................. ................................................................................ ..............i........N........................................N............... .........................................i...................................... ..............................................................................N. ...............................................................N....N.....N..... N..N....N..........N.............................. Pos Score Pred ------------------------ 5 0.253 - 20 0.736 Yes 136 0.178 - 165 0.308 - 184 0.311 - 246 0.528 Yes 271 0.275 - 291 0.724 Yes 295 0.400 - 306 0.842 Yes 459 0.602 Yes 489 0.750 Yes 526 0.447 - 553 0.448 - 576 0.618 Yes 582 0.723 Yes 592 0.526 Yes 669 0.722 Yes 676 0.136 - 688 0.209 - 751 0.416 - 895 0.631 Yes 904 0.342 - 945 0.458 - 1002 0.539 Yes 1119 0.183 - 1184 0.062 - 1189 0.044 - 1195 0.085 - 1201 0.040 - 1204 0.052 - 1209 0.084 - 1220 0.054 -

Explain the output -- Processing locus 697: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCCATCGTCATCCCCAACACAATCGGACTGACTTTCTCTCCCAGTTGTACATTTGTCATGTCAAGCCAAAGAAACAGATA ATAAACTCTGTTTTTTTATAGTACAACATGTGCTTCAACATTAGTAAAATAGGCCTTCCTAATTGGGCTCACCAATCACA ATGTGGCCTCTTTCGATGGAACGGTGTCGTTTTGGACGTGCCAATTGTGCTTTACCAGAGTGATCAATTCCCAGAAGAAG AAGAAGAAGACACTCCTCCAATGGCGCCTCAGCTTCTCTCCTCCAGTAACTTCAAATCTCTCTTTGACTCTGTCGATACG TTCCTCTTCGACTGCGATGGTGTTATATGGAAGGGTGAAACGCTCATCGATGGCGTCTCCCAAACTCTCGACCTTATCCG TTCTAAGGGTAAAAATGTTGTCTTTGTGACCAACAATTCCGTAAAATCGAGGAGGCAATACGCTGAAAAGTTCCGATCTT TGGGTGTCACTTCTATTACTCAGGATGAGATCTTCTCTTCGTCATTTGCGGCAGCTATGTATCTCAAAGTCAATAACTTT CCTAAGGACAAGAAGGTTTATGTGATTGGTGGAGAGGGTGTTCTTGAGGAGCTGCAGATTGCCGGTTTTACAGGTCTTGG TGGTCCTGAAGATGGTGAAAAGAAGGCACAATGGAAATCAAACTCTCTCTTTGAACATGATAAAAGTGTGGGAGCAGTTG TAGTTGGACTAGACCCCAACATTAACTACTATAAGCTTCAGTACGGGACTCTCTGTGTACGCGAGAATCCGGGATGTCTC TTCATTGCGACCAACCGTGATGCAGTAGGACATATGACGGATCTGCAAGAGTGGCCTGGTGCTGGGTGTATGGTTGCTGC CATGTGTGGATCGACTGAAAGAGAACCAATAGTTGTTGGGAAACCATCTACTTTCATGATGGACTTTCTGCTACAAAAAT TTGGGACGGAAACGTCAAGAATGTGCATGGTAGGTGATAGGCTAGATACTGATATCTTGTTTGGGCAGAATGCTGGCTGC AAAACTCTCCTCGTCCTAACAGGGGTGACAAGTGAATCTAATCTACTAGATAAGGGCAACAAGATAGAACCAGACTATTA CACAAGCACAGTCTCTGATATCATAAAACTGATGGAGTCCCCCTAGTAAGTAAGGGCATCATCATTCATCATCTTGCTTT ACACTTTGGAACAGTGATTTACGTCCTGTTGCAAGATGAAATTTTTCTGATTCTTTGTCTTGGTT .........................................................N...................... ...........................N.................................................... N..............i................................................................ ....................i........................................................... ................N.........i......................i.............................. ..............N................................................................. ........................N...............................N....................... ...................N............................................................ ...........N..................N.........................N....................... .........................................................................N...... ...................N.............N...................................N.......... ....................i.....i..........................................i.......... ................................................................................ ...............................N................................................ ...................................N............................. Pos Score Pred ------------------------ 58 0.232 - 108 0.428 - 161 0.475 - 176 0.511 Yes 261 0.871 Yes 337 0.238 - 347 0.514 Yes 370 0.575 Yes 415 0.356 - 505 0.145 - 537 0.366 - 580 0.318 - 652 0.125 - 671 0.388 - 697 0.303 - 794 0.416 - 820 0.264 - 834 0.451 - 870 0.484 - 882 0.282 - 936 0.270 - 939 0.459 - 981 0.796 Yes 987 0.610 Yes 1030 0.657 Yes 1152 0.338 - 1236 0.033 -

Explain the output -- Processing locus 698: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACTTTCCTAAACGGGAATGAGCATGTGATTTTATTTTATTTTACTAGTGTTTAGTGTGACGTCACTTTTGATTGCACTA AAAAAAAGGTGACGTTTTTAACACACATACCTCATGGTGTCTCATGACAATAAAACAATCAGAAGTTCATGCATTTGGTA TAAAAAATTGAAAATTTATTCCGAAGAAAAAGGAGAAAAAATGCATGCGATTTTCTCGAGCTTCTCTCGCAAGGTCGTCG TCAACGTTGGTGCTTCTTCGCAATCGCAGCTAACCAAAATGGTGAAGAAGAAGCCAAACCAGAGTCGCCATCTTCTTCCT TCTCGCCTCTCATCTCCTTCTTCAGTACCCCACTTTGTTCCTTCTGCTGTATCGCGATCGGCGAAAGTTCATGGGTTCTT CGCTAGCAAGTTGGGAAACACCAACCTAAAGCTGAAATTTGGAAATGTTATGGAATCTAGAGCTGGGTTCTTCAGTTCTG AGCTTCCTAGTCATGGATTCGAGTCTGGTGGTTTTACTGGGTTTCAAAAGCGGGGATGGAAGTCTTGGATCAATGGAGCT AATGGTGTAGTTTTTGGACTGCTATCAACGTACAGTTTTACGAGTGGATATATACACACGCTGATAACTTCGGGTTTTAG TCATATTGGTACCAGTCAAATCATCTTGAACATGATTGGAATCTCCTACTTCGGCTCCAGAATTGCAAGAACCTTGGGAC CGCTCTACCTTTTGAAGCTGTACTTTGCTGGAGCACTTGGTGGCTCTGTTTGCTTCCTGAGTTATCACGCCCTCTTGGCT ACACTCAAGGGTGAAGGAGTTGTCATTAAGGATCATCAATCAACAGCCCCCATTTCGCAGCTGCTGGGTGCTGATGGATC TATGTTTGCCATCGCGCTTCTCGATATGTTCATCTACCCAAAAGTTACAACATACTTTGCGTTGATGCTCCGAGTGCATG TAATGTTTCGAATCATAAACTTAGGAGTCGAGATTTTAAATATTCCAGAGGGGGGACCGAACCATATCGCATCGAGTTCG GGTCAGTTGGGAGGAGTTGTGGTTGCTGCCATGGCGTGGGCACGGATAAAGAAAGGTCGATTTTGATACTGAACTGATTT GCTGCAAAAGATTCAGTGGACCTGATCATCATCATGCAAGTTATGTTAAACTTCGTGACAAATGAGATTTTTGTCAACAT TTTTT .................N.....N........................................................ .................................N.........N........................N........... ........................................i...N................................... ......................................i......................................... ......................................................................N......... ............................................i....N.............................. ............N..........................................N................N....... ...............................N................................................ ................................................................................ .........................................................................N...... ..N............................................................................. ..............................N................................................. .................................N........N..................N.................. ..... Pos Score Pred ------------------------ 18 0.433 - 24 0.180 - 114 0.379 - 124 0.308 - 149 0.199 - 201 0.916 Yes 205 0.234 - 279 0.563 Yes 391 0.339 - 445 0.639 Yes 450 0.189 - 493 0.304 - 536 0.376 - 553 0.421 - 562 0.107 - 672 0.426 - 874 0.113 - 882 0.332 - 906 0.354 - 945 0.460 - 958 0.322 - 963 0.191 - 1071 0.302 - 1154 0.082 - 1163 0.050 - 1182 0.044 -

Explain the output -- Processing locus 699: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACAATTTTGATCAGGCCAACAAACTTGGAGAAGGAGGCTTCGGATCCGTATTCAAAGTATGCTATATACCAACTTCTT CTCAAACGAAATATGGCTTAAGGGTTTATTGATATGAGAAACTTAATGCAGGGAGAGCTGTCGGATGGAACTATCATAGC AGTCAAGCAGCTTTCTTCCAAGTCAAGTCAAGGAAACCGCGAGTTTGTGAATGAGATAGGCATGATTTCAGGTCTGAACC ATCCTAACCTTGTCAAGCTTTATGGATGCTGTGTCGAGAGAGATCAATTGCTGCTCGTGTATGAGTACATGGAAAATAAC TCCCTTGCTCTTGCTTTGTTTGGACAAAACTCCTTGAAATTGGACTGGGCAGCAAGACAAAAAATATGTGTTGGAATCGC AAGAGGGCTTGAATTCCTCCACGATGGATCAGCGATGAGAATGGTTCACCGTGACATAAAAACCACTAATGTGCTTCTAG ACACCGACCTTAATGCAAAAATATCTGACTTTGGATTAGCTAGGCTTCATGAAGCAGAACACACTCACATTAGCACCAAG GTTGCAGGAACCATCGGATATATGGCTCCAGAATATGCATTATGGGGTCAACTGACAGAGAAAGCAGATGTGTACAGCTT CGGAGTTGTGGCAATGGAAATTGTTAGTGGAAAGAGTAATACCAAACAGCAGGGAAATGCTGATAGCGTCTCGCTTATCA ATTGGGCGCTGACGCTGCAACAGACAGGGGACATACTGGAGATTGTAGATCGAATGCTTGAGGGTGAGTTCAATAGAAGT GAAGCCGTGAGGATGATCAAAGTTGCTCTCGTTTGCACAAACTCATCTCCTTCCTTAAGGCCAACAATGTCAGAGGCTGT GAAGATGCTAGAGGGAGAGATCGAAATAACACAGGTTATGTCAGATCCTGGTATATATGGGCACGACTGGAGCATCTCAA AGCTGAGGGACATTGATACGCATAGTAGCTCGAGCACGTCTGGTGTGACCGATCAGACGACAACAACAATGAAATCCTCT GTTTCTGGTTGTGATCTCTATCCGTTATACCCTGAATCCATGATCTTAAACTCCACAGTAGAGAATTCTTCCTCGTCACT GTAATATCAAAAGCTATGATACATTGCCTACTTAATGAGAGGCTAGTGAGGTTATCCGAAAGTTAACTACGTAGTTATAT CATTAAAGTTGTCTGGAACCTTA ............................................................N................... ............N....................N...........i..................N............... ..................................................N..........N.................. .....................N...i..................................N.......N........... .................................................................i.............. .......................N..........N.....i...........................N........... ............N...................................N............................... .....................i............N......i.........................N............ .............i..........................................N....................... .....................................................N.......................... ............N.....................................................i............. ....N................................i..................N....................... ....................................................................N........... .......................................N........................................ ...............N..................N............................................. ....................... Pos Score Pred ------------------------ 61 0.194 - 93 0.486 - 114 0.485 - 126 0.559 Yes 145 0.202 - 211 0.383 - 222 0.479 - 262 0.249 - 266 0.545 Yes 301 0.301 - 309 0.354 - 386 0.700 Yes 424 0.225 - 435 0.337 - 441 0.631 Yes 469 0.353 - 493 0.123 - 529 0.204 - 582 0.619 Yes 595 0.493 - 602 0.515 Yes 628 0.360 - 654 0.631 Yes 697 0.155 - 774 0.481 - 813 0.328 - 867 0.836 Yes 885 0.340 - 918 0.569 Yes 937 0.391 - 1029 0.397 - 1080 0.340 - 1136 0.080 - 1155 0.058 -

Explain the output -- Processing locus 700: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTAAAATCGATCCGAAGAAACCAAACCATATATACATGAACCGGAAAAATGAAAAACCAAAATTGAAAAACCATATCAA ACGAAACCGAATCAGAATTGAACACCCAAAAGCCAAAACCCTAATAAATCGTTCAATCAAACCGTATATACTGGTTTTCT AAAAAATATTCGGTTTAGTATGGTTTTTCCCGGTCCGTACTTCTCAAAAGAGAAAATACTTGCAGGCATTGAACAAACAA TGGAGAAAAGAGAAGAAGCAGGAGAGTATCATTCCAAAGATTTCGAATGGGAATTTCTCAAGAACATCGTCGAGAACGAT CCTTCTCTCTCCCACCATCTCCACCACCACCACCAAAATCTCTCCAAATATTTACCAGATTCGCAGCCATGGCAAGATTT TCACTCCCGTCACTCCTCCGGAAAATTCTTCAAGGAAAGAAGGTATCTGTTGAAGGAATTTCCTGAATTAGTTTCTTGTG GAGAGAACTCAAAACTTTTGGAAATTGGTTGTGGTAATGGCAGTACCGTGCTTCCGATTTTACGTGGAAGCAAGAACATT ACTGTGTATGCTTGTGATTGTAGCAGTGACGCTCTTGTTAGGACTAAAGAGAACATTGATCGCGCTATTTCTTCGGTTGA TAACTTTCATTCTTTTTGTTGTGACTTTTCGACTTCTGAGTTTCCAGATTGGGTGGCATGTGATCGTTGTCGAGACAAGT TTATGCTTAACCACTCAGGAGGGAGTGAGAGCAAACATTGCATCGGTGGGGTGGATTTTGTCACTCTGATATTTACATTG TCGGCAGTGCCTAAAGAAAGAATGCCGAGAGCTATCAAGGAATGCTTTGCTGTGCTGAAACCTGGTGGTCTGCTCTTATT TAGAGATTATGGATTATATGACATGACTATGCTACGGTTTGAGCCAGAGAAACGTGTAGGATTTAGGGAATATGTGCGGT CTGATGGAACCCTTTCCTATTTCTTTTGTCTCGACACTGCCAGGAAACTATTTACTGATGCTGGATTCATTGAGGTTGAG CTTGAATATTGTTGTGTCAAAGCAGTAAACCGGAGAAAGGGTAAGGACATGTACCGAGTTTGGGTTCATGGGAAGTTCCA AAAGCCCTTCTCTAATTAAGTTGAAACGCAATGCTCTATCTGTGAAGAAGATTGCAGAGTCTAGTGTTTGGATTATCTTT ACACTGATCCTCTCATACTAAGTTCACGTTCTTTGTTC ....................................N............i.............................. ................................................................................ ...................i...........................................................i ..............................................N................................. ....................................................................i........... ................................................................................ ....................................N........................................... .......N........................................................................ .........................................................N...................... ..i............................................................................. .....................N...................i...................................... ........N........N....i.....N..........................................N........ ...N.....................................................N...................... ................................................N..................i............ ..............................N................................................. ...................................... Pos Score Pred ------------------------ 37 0.314 - 50 0.655 Yes 180 0.638 Yes 240 0.627 Yes 287 0.340 - 389 0.612 Yes 517 0.346 - 568 0.127 - 698 0.199 - 723 0.595 Yes 822 0.312 - 842 0.558 Yes 889 0.205 - 898 0.202 - 903 0.684 Yes 909 0.306 - 952 0.406 - 964 0.333 - 1018 0.398 - 1089 0.266 - 1108 0.524 Yes 1151 0.092 -

Explain the output -- Processing locus 701: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATAGTGTCATCCTCTCTGGTTTTTGGAAAAGGAAAAGTTGCAATGAATAAAAGCAAACAAGAGAATTGCATCTCATCA TTATGCTCTCGCTTTATTTAACAAAAACTTTTTCTTTGGCCAATGATCGATCCTTGCTCTACATATATACAAGTATTATC ACGAGTAAGACCCCAAACAAAAATGGGAGTTTCCTCTCCCGACTTCCGAGCCCCTCCTCCGTCCCCGGTAGCATCCAGCC GCCGTGCCTCCTTCACGGCCAACGAAGACGTCCTCTCGGAGTTCCTGGACAAGTGTGGTCGAGTCCCAAATCTTGTATTG CCCGATAAAGTCTTCCCCAAGCACACATTCCTTCTCAACCCTCCCACCTTCGATTTCCACCGGTTGGACTCCCTCTCGGC TCTGCTTGATGCCATCGCCACCATCGGTTGTTTTCAGCTCGTTAACCACGGTGTTCCAGAGGCTATGGTGAAAGCCGCCA TGGACAAAACCATTTTCCACGATGAGACGGAGGAGTTCGTGTTTAATAAAGATATTAATGCTGATGATCACACGGCTTGT TATTCAGATTTGAGGGAGTTGATGGGTGAGGCAGAGAGGATAGGGAAGGCGGTGAGAGAAAAGCTAGGAGCAGGAGGAAG AAGCCAAAAGGAAGGGGTAGGGGTTTGCTACGTGAAGAGGCATAATATTAAAAACGAATCAAAGGAAGAAGCCATAAGGA TGCTGCTAAGAGGCTATGACGAAAGACATTCTCTATGTCTCAACTTCTGCCATGCAGAGTTCCACGTCTACTCCAAAAGA GGTTGGGTCTCCTTCTCCCCACGTCCCGATGCGGTGATCGTCACAATCGGAGATCAAGGCTGGAGCGGAAGATTCAAGGG CGTTGTGGGGAGGCCCCTTCTTTACAAATCAGATCTTCATCACAATCTGATTTCCATATCCTTTCTCTACACTAGTACTA CCACTCTCCACAACACAAGCAGAAAAATTACCAAAAGGACGAAGAAGAAGACCATTTCCCTCTTCCAACAGCTCCTCTTT GCTCTCTTTCTCACACTCCTCTTCCCTTTTTTACTTCCTTGATCATTACATACTTGTTGCCTTGTTGCTCCTTTTTTCCT TTTTTTCTTTGGAAACTAAAATACAAGTGGGTAATGTGTATCTCAATCTTGTTTAATTTTA ............................................N................................... ..N.......................................N..................................... ......................i......................................................... ................................................................................ ................................................................................ ........N.......................................................i..............i .....................N...................................N.....N................ .....................i.......................................................... ...............................................................................N ...............N..................i................N............................ ............................N................................................... ................................................................................ ................................................................................ ................................................................................ .................................N........................... Pos Score Pred ------------------------ 45 0.396 - 83 0.174 - 123 0.413 - 183 0.804 Yes 409 0.342 - 465 0.758 Yes 480 0.718 Yes 502 0.189 - 538 0.463 - 544 0.229 - 582 0.714 Yes 720 0.404 - 736 0.413 - 755 0.648 Yes 772 0.493 - 829 0.217 - 1154 0.067 -

Explain the output -- Processing locus 702: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGAAATGTCAGAATTGAAATAAAGTTTACCATAAAGTTATGGTTTTTATTTCCCATCTTTGGGGAAATGGCAGTTAAA TTCCCGTGTCGATGGCTTTGAAAAGTGGTTGCATTTATGATCCACCCTTTTATTTTAATCACACATCCGAATTACCAATC GTTACCCATTTCTTCAATGAACACTCTGGTGAGGAGCGGTGGCGCGTGGCTGGTATCGCCGTCAATGGCAGTGGCGGCTC TGAAGAGGATGAGAGTGTCTGGTTTAGCCACGTCATCATCATCTTCTGTTCCTAATTGGAAAGGTGGAGTTTCCATGGTC CAAGGAGCTTCCAGAGGCATTGGTCTCGAATTTGTACGACAGTTGCTGGAGAACAATAAGAACGGGTATGTTGTTGCTAC TTGCCGAAATCCAAAAGAAGCAACAAGTCTCTCTGATTTGAAGAACCGTTTCTCTGAAAGACTTTTCATCCAAAAGCTTG ATGTTACAGATGAAACTACCATTGAGGAATCAGCGGAATCTGTGAGAGAAAAATATGGTTCTCTCAATCTACTTATAAAT GCAGCTGGTATACTTTCAATTCCTGGCGTATTGCAGCCAGAAACAACATTGAACAAGGTCGAAAAATCATCGTTGATGCT TGCTTACGAGGTTAACGCTGTTGGTCCAATTCTAGTGATGAAGCATATGTGGCCTCTCCTTAAGGCTGGTGGAGGTAGTG GAACAGATAGAGAAGTTGCTGTTGTAGCCAATTTAAGCGCTCGAGTTGGATCCATTGGAGATAACCGACTTGGTGGTTGG CACTCTTACAGGGCTTCAAAATCCGCACTCAATCAACTGACAAAAAATGTATCGGTTGAGTTAGGTAGGAGGAAAGATCC TGTGGCATGCATTCTACTACATCCAGGCACAGTAGACACTGATCTCTCTCGGCCGTTTCAAAGGAATGTTCCTGACGGAA AACTTTTCACAAGAGAGTACTCTGTTCAGAAGTTACTGCACATCATCAACAATACTAAGAAACAAGACAGCGGCAAGTTC TTTGCTTGGGATGGCCAGGAGATACCTTGGTGATTTCTTATATGGTTTTCTCGATCCGAATGGTCAGTTCTTTCAAAGTC TATTGAATTCTTGTCGGGGTTTTTTCACGTTCTTGGTTTGAGAGCAATTCCT ......N.................................N...........................N........... ...........i........................N........................................... ................i...............................................i............... ........N.................................................................N..... ...................................................................N............ ................................................................................ N........N............................................N.......................N. ...........................................................................i.... .....................................i........i................................. ................................................................................ ..............................................N................................. ......N..........................................................N.............. ................................................................................ ..........N..............................N.................N.................... .................................................... Pos Score Pred ------------------------ 7 0.409 - 41 0.378 - 69 0.400 - 92 0.523 Yes 117 0.332 - 177 0.541 Yes 225 0.591 Yes 249 0.237 - 315 0.486 - 388 0.205 - 481 0.155 - 490 0.146 - 535 0.182 - 559 0.222 - 636 0.655 Yes 678 0.695 Yes 687 0.722 Yes 847 0.375 - 887 0.455 - 946 0.178 - 1051 0.241 - 1082 0.306 - 1100 0.062 -

Explain the output -- Processing locus 703: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTAATCCACATGGGGAGAGGAGAAAAAGCATAATGGAAGGAAAATAGAAAATAGGTTTCTCATTGACTTGAGCGTTTCC AATACATAGAGCAATAAATAGATGTCTTTATCTTTGATAATTACAGAACCAGAGAACTCTTTTTCTTTTCCCTTCTTCTC TTTCAAAAAACAAAACAAAGATATCTCAACCAAAACACTTAGACCAAGAGAGACAGATAGAGAGATGGGTAGGGCTCCAT GTTGTGACAAGGCAAATGTGAAGAGAGGTCCATGGTCTCCTGAAGAAGACGCAAAGCTTAAAGATTACATCGAGAAACAA GGAACTGGTGGCAATTGGATTGCTCTCCCTCACAAAGCTGGTTTAAGGAGATGTGGGAAGAGTTGCAGACTGAGATGGTT AAATTATTTGAGACCAAACATAAGACATGGAGATTTCACTGAAGAAGAAGACAATATTATCTACAGCCTCTTTGCCTCCA TTGGAAGCAGGTGGTCAGTAATAGCAGCTCACTTGCAAGGTAGAACTGATAATGACATCAAGAACTATTGGAACACTAAG CTCAAGAAGAAGCTCATAGCCACCATGGCTCCTCCTCCACATCACCACTTAGCCATTGCTACATCATCATCATCAGCATC CCCATCATCATCATCACATTACAACATGATCAATAGTCTTCTTCCGTATAACCCATCAACAAACCAACTTCTCACACCTC ATCAGGGTATCATGATGACAATGATGGGCCAACAACAACAACTATTTTATCAAGAAGACATGGGCAATTTGGTAAATTCT CCAAACAGAAACAATCTCATAATGAGCCATCAAGAAGACAACCAAGAGCAAAGTACAAACAAGGGAATAATGTTGTTGAG TGATGTAAGAAGTGGGTCGAGTACAACAAGTACAGTAACAAGAGTGAAGATGGAACATCGTGATCATGATGATCATCATC ATCATCATGAAGAAGATGAGAGATCAATGACCTCGGTAGTGATGGAAGATTATGGAATGGAGGAGATCAAGCAATTAATA AGTAGTAGTTGTACGAGTAGTAACAATAGCTTGTGGTTTGACGAAAACAAGACGGAGGATAAGTTCATGTTGTACTACTG ATCTCGAGTACCAACTTTGATTTAATCTCCATATATAGTCTCTCTCTTATCTCTTTCCTTGTCAAAACCAAATATGAATT ATTAAAACTTTGTTTTGAGG ..........N......................N.............................................. .....................N.......................................................... ................................................................i.............N. ...............N...............N................................................ ..................................................N.......................N..... ..........................i..................................................... ...................................................N............................ ........................N....................................................... .........................N...................................................... ...........N..i.....N..i...................................i.................... .....................i...............................................i.......... ..N..............................................i...............N..N........... ......N........N..........i..............i.........N....i....................... ..................................................................N............. .........................................................................N...... .................... Pos Score Pred ------------------------ 11 0.428 - 34 0.430 - 102 0.293 - 225 0.788 Yes 239 0.220 - 256 0.368 - 272 0.147 - 371 0.169 - 395 0.209 - 427 0.526 Yes 532 0.165 - 585 0.318 - 666 0.323 - 732 0.227 - 735 0.507 Yes 741 0.457 - 744 0.738 Yes 780 0.546 Yes 822 0.575 Yes 870 0.651 Yes 883 0.453 - 930 0.520 Yes 946 0.318 - 949 0.085 - 967 0.078 - 976 0.100 - 987 0.544 Yes 1002 0.678 Yes 1012 0.257 - 1017 0.687 Yes 1107 0.238 - 1194 0.095 -

Explain the output -- Processing locus 704: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACATGATTTGGGCTTTGTAATTCTAATTACAACATCAGGGGCATTTTGGTAATAACATCAAATTCGAAATGCGAGAGAA ACTTGGGGAGTGGGGTACTGAAAGTGGAGGGTAATGGTAATGTGGGTGATAACCGCATCCAACCGCCACTACAAAACCCA AAGAAACTTCCGCACACAAAAACAGAGAAGAACAAAAACACAAAATCGAGAGAGAGATCGATTAGCGATGAGGAAAATGG AAGCGAAGAAGGAAGAGATAAAGAAAGGTCCATGGAAAGCCGAAGAAGACGAAGTACTCATCAACCATGTCAAGAGATAC GGTCCTCGTGATTGGAGCTCCATTCGATCCAAAGGTCTTCTTCAACGCACCGGCAAATCCTGTCGTCTTCGTTGGGTCAA TAAACTCCGTCCCAATCTCAAAAATGGATGCAAGTTCTCGGCTGACGAAGAGAGGACTGTGATTGAGTTACAATCTGAGT TTGGTAACAAATGGGCGAGAATCGCTACGTATCTACCGGGAAGAACTGATAACGATGTGAAGAATTTCTGGAGTAGCAGA CAAAAGAGACTCGCTAGGATTCTTCATAACTCCTCTGATGCATCGAGTTCGAGTTTCAATCCCAAATCTTCTTCTTCTCA TCGACTCAAGGGCAAAAACGTCAAACCAATCCGTCAATCCTCTCAGGGTTTTGGTTTGGTTGAGGAAGAGGTTACAGTTT CTTCTTCATGTTCCCAGATGGTTCCTTATTCATCTGATCAAGTTGGTGATGAAGTCTTGAGGTTGCCGGATTTGGGTGTT AAGTTAGAGCATCAGCCTTTCGCTTTTGGCACTGATCTTGTCCTAGCAGAGTACTCTGACTCACAGAATGATGCAAATCA GCAAGCAATCAGCCCTTTCTCTCCAGAAAGCAGAGAGCTTTTGGCTAGACTTGACGACCCTTTTTACTATGATATACTTG GACCAGCTGATTCTTCTGAGCCATTGTTCGCTCTCCCTCAGCCGTTCTTCGAGCCTTCGCCTGTGCCGAGAAGATGCAGA CATGTTTCAAAGGATGAAGAAGCTGATGTTTTCTTAGACGATTTCCCAGCTGACATGTTTGATCAGGTTGATCCAATCCC AAGTCCTTAGATTGTTGATTTTTTGTTTGTAGATGGTTTCCTTTGTGTGTTTAGGTCTATAATCTAACTACATTCTTATG TAATGACAATTCTTGTTCCCTTGTTATTA ...N.................................................................i.......... .................................N.....N........................................ ...................................................................i........i... ...............................N..................................i............. ................................................................................ .......................i...N.................................................... ..........N...........................................N......................... .....................................N.......................................... ................................................................................ .......N.........N..............................N............................... ...................................................................N..N......... ....................................................................N........... .........................................................................N...... ................................N............................................N.. ..N.......................... Pos Score Pred ------------------------ 4 0.288 - 70 0.731 Yes 114 0.311 - 120 0.387 - 228 0.617 Yes 237 0.597 Yes 272 0.251 - 307 0.652 Yes 424 0.667 Yes 428 0.326 - 491 0.475 - 535 0.378 - 598 0.263 - 728 0.140 - 738 0.283 - 769 0.212 - 868 0.335 - 871 0.218 - 949 0.199 - 1034 0.182 - 1042 0.195 - 1054 0.119 - 1066 0.107 - 1095 0.515 Yes 1153 0.065 - 1198 0.063 - 1203 0.063 -

Explain the output -- Processing locus 705: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACCTCAAAACAAAGACTTGTCTAAAAAGTCAATGACTCTCTTCAGTCATATTTTATTGTTTCTAAAGAAAATGTCTTC TCTTTTATATATTGCTATTAGTTCTCTTTTCTTCTATATATGCAAATCTCATTTCTGGCTTCCATACAAATAAACAACTC TGAAGTCAACAATCTTTATCCAAAGAAATTCATCATCATGGAAACCATAAGAGAAGAGGCTCCTTCAAGCTACTTGATGA AGCTGGTTGGTTTCTCTGAGGTTAAATTCAGTCATCAACCTTATGAATCTGCTGATTTCGACGTTGGGGGACATAAATGG AGATTGATATTTTACCCGGCTGGAAAGTTAGAAGAAGGTGGAAAAGACCATGTTTCGATTTATGCGAGGATCGAAAATGT TGGTGCTTCTATGCAGATTGATGCTGAACTCAAGTTCTTCATCTACAATCACAACAACAAGCAATACTCTGTCTTTCAAG ATGGAACTATGAAGCATTACAATAAGGAGAAAAAAGAGTGTGGATTTGCGCAAATGCTTCTTTTTTCGAAGTTCAACGAC CCAAAGAATGGGTACATCGATGGAAATGCTTGTATCGTTGGGGTGGAGATATTCGTTATAAAGCCGATAGAGAAGGTGGA GAGGGTTGTATTCACACAAAACCCACCAGAAAACAAATTCACGTGGAAAATCTCTCATTTCTCATATATAGGAGACAAGA GATACTATTATTCAGATGAATTTGTGGTCGGAGATAGAAAATGGAGATTGAAGATTAGTCCAAAGGGAGACAAAAAGGTA AGAGCACTTTCAGTTTATGTACAAGCCATGGCATATCTGCCAAATGCGGTAGCTTCTAGCACTTATGCAAAGCTCAGACT TCGGCTACTAAATCAGAAAAACTCTAACCACATTGAGAAACGCGTTTTTCATTTTTACTCGAGAGAGAATGGAGACGGAT CAGGGATATCTGAGTTAATATCTGTGGAGGATCTAAATGATGAATCAAAAGGGTACCTTGTGGAAGACTCCATTGTTCTT GAAACTACTCTTCTTTGGGTCTCTGACACTAAGGTCGTTGACTCTATCTAAAGACTCCTATTACCTCTTTCTTTCTTTTG CATACATCTTTCTTTTTTAAGCAACAAAATGTCTTCTTTGCTATTTCCCCTTTTTTTTTTTTTTTTTTTT .................................N......................................i....... .......................................N........................................ .....................................i......................................N... ..........................................N.................................i... .................................................N...........N..............N... ..........N.........N........................................................... N.......i............................................i.......................... .......N...........N.....N...................................................... ................................................................................ ...............N........................i....................................... ................N..........N...............N....................N............... ....................................................................N........... ....................................N..N........................................ ................................................................................ ............................N......................................... Pos Score Pred ------------------------ 34 0.353 - 73 0.588 Yes 120 0.349 - 198 0.551 Yes 237 0.399 - 283 0.184 - 317 0.559 Yes 370 0.225 - 382 0.176 - 397 0.204 - 411 0.341 - 421 0.339 - 481 0.357 - 489 0.641 Yes 534 0.524 Yes 568 0.401 - 580 0.128 - 586 0.266 - 736 0.136 - 761 0.755 Yes 817 0.176 - 828 0.337 - 844 0.325 - 865 0.221 - 949 0.197 - 997 0.451 - 1000 0.354 - 1149 0.097 -

Explain the output -- Processing locus 706: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATAAAATCCCAAGAGTCACTTTCTATATTCGACACATTAATATTGATTTTCTTCCTAATCCAAATTACGTTGACCATAT CATACATTAATTATTTACTAGTTGGAATTATATAATATGAAAAAATTCCGAGTCACTTTAAGCTTTTCGAGGTATCTCCT CCGCGCGACGATGTCAGAGACTCGCCGGAATCAGTCTAGCGATGAACAAATCGAAAAATCAGAGATTCAGCACCAAAGAG TTGTAATTGAGAACAGTCATGGTGAGAAACTCGTAGGAGTTTTGCATGATACTGGTTCTACAGAGACTGTTGTCATCTGC CATGGATTTCGTTCATCAAAGAATCGGATTCCAATGCTGACCATTGCAAGTTTCTTTGAAAGAGCAATGATCAGTTCTTT CCGGTTTGACTTTGCTGGCAACGGGGAAAGCCAAGGATCATTTCAGTATGGTAACTATCGCCGTGAAGTTGAAGATTTGC GCTCTGTTTTGCAACACTTGCGTGGTGTTAACCGTGTGATTTCTGCGATTATCGGACATAGTAAAGGTGGAAATGTGGTG CTTTTGTATGCAGCAAAGTACAATGATGTGCAGACTGTTGTGAATATCTCAGGGCGGTTTTTTCTTGACAGAGGAATCGA ATTTCGGCTTGGTAAGGACTACTTCAAGAGAATCAAAGATAATGGATTCATTGATGTTAGCAACAGAAAGGGAAAGTTTG AATATCGGGTAACAGAAGAGAGTCTAATGGACCGTCTCACAACCAATGCTCATGAAGCATGTCTCTCAATTCGTGAAAAC TGCAGGGTCTTGACAGTCCATGGGTCAAATGATAGGATTGTGCATGTAACAGAAGCGTCCGAATTTGCTAAACAGATAAA GAATCATAAGCTGTATGTTATTGAAGGAGCTGATCATGAGTTCACTTCTCACCAGCATCAGCTTGCTTCAATCGTTCTCT CATTCTTTAAATTGGATCCTAAGAAAGATGATGAGTCAGGTGATATCAGTACAAGTAACCAAGACTCCATGAGATCAGTT GTTCCTATTACATCGCGAATTTAAATCGCTTTGCTGAGTTACTTAATGTCTCACGGTCTCGGGGGTGATAATAAACGGTT TGATAAACACAGTTAGAAACCAAAATGTTCTTATGGATAAAAT ................................................................................ ....................................i........................................... ..........i..............................N...................................... ..................N..........................N.................................. ...............................................N................................ ........................................................................N....... .......N..............N..N...................................................... .........................................N...........N.......................... ..........................i..................N.....N......i..................... ...................N........N..............N.................................... ..............N....................N............................................ ...........................N..N.....................................i........... .............................................N.................................. ........................N.......N.......... Pos Score Pred ------------------------ 117 0.611 Yes 171 0.747 Yes 202 0.139 - 259 0.406 - 286 0.165 - 322 0.431 - 354 0.770 Yes 387 0.504 Yes 448 0.229 - 553 0.457 - 568 0.280 - 583 0.362 - 586 0.280 - 682 0.188 - 694 0.195 - 747 0.618 Yes 766 0.165 - 772 0.200 - 779 0.600 Yes 820 0.092 - 829 0.132 - 844 0.196 - 895 0.133 - 916 0.359 - 988 0.231 - 991 0.212 - 1029 0.620 Yes 1086 0.099 - 1145 0.063 - 1153 0.055 -

Explain the output -- Processing locus 707: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCGTTTGGATACCCGGTTATATTTATCTTTAACCGGGATACTAATATACGTGATTGAAATTAATTCGGTTTAATAAACC GATTGATAACCACCGGTTTAAAAAGTATCAAAACGAAGGAGTGAATGGATGAGAGCTAAAGGGAAGAAGCAAAGTGAAGA AGCTCCAGAACACGCCATAGCTATGGCTCTTCTCTGTCCTTCTCTTCCCTCTCCGAATTCTCGTCTTTTCAGGTGCAGGA GCAGCAATATTTCGAGCAAGTATCATGGAGCCTCAAAGGAGCTGATGATTGCTCGTTCTGGTGTGTCTACGAGATCAATT TCTTCGGAAAAGGGGCTTTCTCGTAGGGATTTAGTGCTCATTGGTCTCTCTTCTCCGTTGTCTATGTTCTTGCCATTGTC TTCTCCAGTCACTCATGCAGAAGAAGACGTGAAAATGAGTGGAGAAGAATTGAAAATGGGTACAATGGTGGATGATATTA ATGCTTATTCTTATGCTTACCCATTGGATTATCCATCTGAGAAGCTTGTCTTCAAATGGGTTGAATCAAGAAAGCCTGAA AGATACTCTTCAGCTGCACCACTCTCTCCTGATGCACGTTTACGGATTGTTTCTGAACGAGTTGACCTAACTGACAACCT CGTCATTTCTATTTCGATAGGTCCCCCAAACTCAAGATTAACATCAAAAGAAAAGAAAACATGGTCCGCTAAAGAAGTTG CTGATTCTGTTTTGTCTGATAAATCAGCATTGCGTGTCACCTCAAGTCAGCGTCTTGAAGAGAGTTCGGTTCTTGATGCT CATGCTAGTGATATTGATGGGGAGCCTTACTGGTACTACGAGTACCTTGTCCGCAAATCACCAACCAAAATTGCTGAAGC ATCAAAGCTTTACAGACACTATATTTCTTCAACAGCTGAACGTGATGGATACTTATACACCATAAATGCTTCAACCCTTG GCAAGCAGTGGGACAAGATGGGACCGGTGTTAGAAAGAGCAGTAGGATCCTTTAGGCTTCTTCCTCCTACTGATAGTTAT GTTCCTCCATACAAGGATCCATGGAGGTTTTGGTGATGCTTCCATCTGCCTCATGAAATTTCTTTTTTCGAAATTGTCGT TAATTTTGATAGGAGAGGAGGTTATGAAAGTTGTAGTGTGAGAAATGAGCCAAGC ................................................................................ ............................................i...N............................... ......................i......................................................... ........................N...................N................................... ...............................................................N................ ..............N...................i....................i........i......N........ i...........N..........................................N........................ ...............................N................................................ ............................................................N................... ...........................................................................N.... ............................................N....................N.............. .................i............................................................N. ....................N..............N................N........................... .......................N....................N.......... Pos Score Pred ------------------------ 125 0.521 Yes 129 0.388 - 183 0.521 Yes 265 0.363 - 285 0.411 - 384 0.320 - 415 0.207 - 435 0.841 Yes 456 0.714 Yes 465 0.838 Yes 472 0.209 - 481 0.517 Yes 493 0.159 - 536 0.278 - 592 0.180 - 701 0.364 - 796 0.266 - 802 0.189 - 817 0.448 - 925 0.194 - 946 0.258 - 978 0.557 Yes 1039 0.269 - 1061 0.399 - 1076 0.157 - 1093 0.098 - 1144 0.046 - 1165 0.060 -

Explain the output -- Processing locus 708: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTTCCTTATTCCTCTCAAATGCGTGTCCTAGAAGTTCCACAAGTCTAACCAAGACGTATACACAATGATACACAATAAT AATTCATTTTCCAAACGTTGATGGTATTTGAGTAATTTCAAATCTCTACAGAAAAATATTTCTCTTTCCCTATATATATC CGATACCATCTCATTGCACTTTCAACCTCAAACAAATAACCAATCAACCAAAAAAAAAAAAAAAATGAAGAAACTTCTTT TGATATTTCTCTTTTCTCTTGTCATTCTCCAAACTGCTTGTGGATTCGATTACGACGACAAGGAAATAGAAAGCGAAGAG GGCTTATCAACGCTTTATGATAGATGGAGGAGCCACCACTCCGTTCCTAGGAGTCTAAATGAAAGGGAGAAGAGATTCAA CGTTTTCAGACACAATGTCATGCATGTCCACAATACCAACAAGAAGAACCGATCCTACAAACTTAAGCTCAACAAGTTTG CAGATTTAACAATTAATGAGTTCAAGAATGCATACACCGGTTCCAACATCAAGCATCACAGAATGTTGCAAGGACCAAAA CGCGGCTCAAAACAGTTCATGTATGATCATGAGAATTTATCCAAATTACCGTCCTCCGTTGATTGGAGAAAGAAAGGTGC TGTCACTGAAATCAAGAATCAAGGAAAATGTGGAAGTTGTTGGGCATTCTCTACCGTAGCAGCTGTTGAGGGAATCAACA AGATCAAGACTAACAAACTGGTCTCACTGTCTGAACAAGAACTAGTGGATTGTGATACTAAGCAGAATGAAGGTTGTAAC GGAGGTCTTATGGAGATTGCATTTGAATTCATCAAGAAGAACGGTGGTATCACCACTGAAGATAGCTATCCTTATGAAGG TATCGATGGAAAATGCGACGCTTCAAAGGATAACGGCGTGCTTGTGACGATAGATGGGCATGAAGATGTACCCGAAAACG ACGAAAATGCTCTTCTTAAGGCTGTTGCAAACCAACCTGTATCTGTTGCAATTGATGCTGGAAGTTCAGATTTCCAGTTT TATTCAGAGGGAGTGTTTACGGGATCTTGTGGAACAGAGCTGAACCATGGTGTGGCAGCGGTCGGGTATGGATCTGAGAG GGGGAAAAAGTATTGGATAGTGAGAAACTCATGGGGAGCTGAATGGGGAGAGGGAGGCTATATAAAGATTGAAAGAGAGA TTGATGAACCGGAAGGGCGTTGCGGTATTGCGATGGAGGCATCGTATCCGATCAAGCTATCGTCGTCTAATCCTACCCCG AAAGACGGTGATGTCAAAGATGAGCTCTAGATATGTGTACACATTGTGTAAAACCATATCAAAGGTGATCCCAATTCCTT GTTGATTCATGTTGTTAGTTTCGTCTTCATGATTTGATTTTGTTGTTGG ...................N.............................................N.............. ....................N........................................................... ................................................................i............... ................................................................................ ................N......i..................................N..................... ..............N....i...N........................................................ ...............N...........i..................................i................. ..................N...N.....N................................................... ...........................i.................................................... ..................................................................N............. .........i...............................................................N...... .....N......N........................................N.....N.....N.............. ......N...............................................N......................... ..............................................i....................N............ ..............................N...........N..................................... ...N............................N............................................... ..........N........N............N............................................... ........N...................N.................... Pos Score Pred ------------------------ 20 0.415 - 66 0.336 - 101 0.218 - 225 0.778 Yes 337 0.409 - 344 0.583 Yes 379 0.209 - 415 0.324 - 420 0.520 Yes 424 0.178 - 496 0.453 - 508 0.523 Yes 543 0.513 Yes 579 0.301 - 583 0.297 - 589 0.360 - 668 0.791 Yes 787 0.181 - 810 0.658 Yes 874 0.113 - 886 0.101 - 893 0.470 - 934 0.177 - 940 0.089 - 946 0.249 - 967 0.239 - 1015 0.175 - 1087 0.730 Yes 1108 0.218 - 1151 0.373 - 1163 0.267 - 1204 0.186 - 1233 0.365 - 1291 0.174 - 1300 0.130 - 1313 0.291 - 1369 0.032 - 1389 0.035 -

Explain the output -- Processing locus 709: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAATATATATTTTATCATTTTTTTTGGAACAAAATATATATAATTTAGCCTATTTTCGTAATTCTCCAAAAAGAAAAT GGTCGTGTAATATCGTCGTAGTTCGTACTCCACATGCACCACAAAACTGATCGGGAGATAAGAGCGGTAAAAACCACCGA GTCACGACTCAGTGACTCAAATCGTTCTTCCCACACAAAAAAAATGGTTACTGCGACAGCATCAACTCCATTTTCACTCT CGTCGTCCGTGGTTTTCACGCGCCGTCGTAATTTCAAACGTTTTCCAGTTTGCGCCAGTCTTTCTCCGGAGGTTTCTCCT CTTCTCAGAGCAGCTCATCACACTGTGGATAATTATGTAAAGAGTGGGATGATTATTGGTTTAGGATCTGGAGAAGCTTC AGATTTTGCTATACGTTATTTGGGTCAACAACTTGGTTCTGGTTCTTTACATAATGTTGTTGGTGTACCAATGTCTGCTC GAAGTGCGAGTGAAGCAGCAAAGTATGGAATCCCCTTGGAATATTACCGGGATGGTGTTCAGATTGATTTTGCATTTCAT GATGCTGATGCTGTAGAAGAGAATACGCTTATTGCAGTTATTGGACGGCGGAGAAGTTCACAGGAAGATGACTATATTCT GAAACAAAAGTCTATTGTAAAAGTAGCTGATGAGGCAGTCTTCATGATAAAGGAAGAACAATACAAAGCCGGTCTTGAAG GTTCTATCCCTGTTTTAGTTCAATCTCTTAATTGGTTGGCCATAGCTGAGGAGATAGATGACTTGTATCTAGGCGATGCA GAGGTGTGGAGAAGAGCTTCTGTTGAAAATGAAGGCCCTCTTGGAGGAGACTTTCCTATAGTTACCAGTGATGGTCACAA CATTCTTGATGTTATATTTACAACTCCGATTCGAAGCCTCGCTGACTTGGCTACAAGCCTTGATAAGATTGATGGGGTTG TGGACCATGGACTAATTATCAAAACTCGATGCACGGTGGTGATTGCAGAAGAAACTGTTGTAAGAAGTGTTACCTTGCAG ACCAGTGCATTGGAGGGTGGTGTACGAACCTATGGACAGACTTTGCAAAAGTAGTTCGTCTCACACATTACCGAAAACGG TGATCCATTTTGCCAGTCAATTTATAAGTCAGTTCATAGAGCAATGCCGTAACTTCTACAAGTTCTTTTTCTA ..............................................................................i. .................................N.............................................. ...........................................i.................................... ................................................................................ ..................................N.............N............................... .....................................................N................i......... ........................N..........................N..........................N. .............................N.............N.................................... .........................................................N.................N.... ............................N.........................................N......... ........N..............................................................N........ ......N.....................N................................................... ...............................N................................................ ...........................................N............................. Pos Score Pred ------------------------ 79 0.771 Yes 114 0.233 - 204 0.662 Yes 355 0.178 - 369 0.422 - 454 0.172 - 471 0.814 Yes 505 0.301 - 532 0.261 - 559 0.074 - 562 0.173 - 568 0.147 - 628 0.165 - 670 0.108 - 684 0.258 - 778 0.291 - 796 0.163 - 829 0.215 - 871 0.148 - 889 0.089 - 952 0.201 - 967 0.477 - 989 0.449 - 1072 0.254 - 1164 0.063 -

Explain the output -- Processing locus 710: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGAATCCCAAAAATATCGACAAGCCCAAAATTTCTTTACATAGCTATAAATACCTACATTCTCTCATCAACAATAATC AATAAGATTAGTAAGATTTAATATAAAAGAGATAAAGTCATGGAACTCTTAAAAAGAAAATTATATGCAAAAATTTTGAT GATGGTAATGGTGATATGGATTGCACCTATGACTAACGGTCATGATCATGCAAGCCACGTACCCGGTGGAAGACCCGGTG CCCACCCATCCCATGGCGCCCACCCAGCCCATGGCGCCCACCCATCCCATGGTGCTCACCCATCCCATGGTGCCCACCCA TCCCATGGTGCTCACCCATCCCATGGCGCCCTCCCATCCCATGGTGGCCAAGTGCCGCATTCTGGTTGGGGTCATGGCCG TGCCACATTCTACGGTGACATCAATGGTGGAGAAACTCAACAGGGAGCTTGTGGATATGGTGATCTACACAAACAAGGAT ATGGTCTAGAGACCGCAGCACTAAGCACCGCACTATTTAACAACGGCTCAAGGTGTGGGGCTTGTTACGAGATCATGTGC GAACACGCTCCACAATGGTGTTTGCCAGGTTCCATCAAGATCACAGCTACAAACTTCTGTCCACCAGATTTTACCAAGCC CAATGACAATTGGTGCAACCCGCCACAAAAACACTTTGATCTCTCGCAACCAATGTTCCTCAAAATAGCTAAATACAAAG CCGGGGTTGTCCCGGTTAAATTTAGACGTGTTCCTTGTGCGAAAATCGGAGGTGTCAAGTTTGAAATCAAGGGAAATCCT CATTTCTTAATGATCTTACCGTACAATGTAGGAGGAGCTGGAGCTGTTAGGGCTATGCAAATTAAGGGAACAAGGACCCA GTGGATTGCGATGAAGAAGAATTGGGGACAGATTTGGAGCACTGGTGTTGTGTTGACCGGACAATGTTTATCGTTTAGGC TTACCACAAGTGATGGAGTTATGAAAGAGTTTATTGATGTTACACCGCCGGACTGGAAATGTAATGGACAGAGTTTTGAT GGAAAGGTTAATTTTTAGAAAATGAATTTGAGTTTTGTTAGTTAATACTTGCAAGTAACTAAGAAGAGAGGGTTACTGGT AGGTCAATTAATTTGTAATATAAAAATATTTGTTGTT ................................................................................ .......................................i........................N.............i. ............i.................i.................i.................i............. ....i.................i.................i................................i...... .......................i................................i....................... N.........................................................................i..... ..............N................................................................. ..N.................................................i........................... ................................................................................ .........N...............i............................N......................... ..........N....................................................N................ ............N.......N...............N.....................N....N..............N. .....................N.......................................................... ..................................... Pos Score Pred ------------------------ 120 0.536 Yes 145 0.353 - 159 0.811 Yes 162 0.810 Yes 168 0.686 Yes 176 0.454 - 189 0.628 Yes 202 0.656 Yes 208 0.558 Yes 253 0.763 Yes 271 0.866 Yes 289 0.659 Yes 307 0.784 Yes 325 0.702 Yes 343 0.770 Yes 361 0.785 Yes 394 0.764 Yes 424 0.654 Yes 457 0.592 Yes 481 0.426 - 555 0.509 Yes 575 0.418 - 643 0.231 - 693 0.688 Yes 810 0.376 - 826 0.617 Yes 855 0.204 - 891 0.167 - 944 0.167 - 973 0.221 - 981 0.283 - 997 0.158 - 1019 0.411 - 1024 0.213 - 1039 0.152 - 1062 0.334 -

Explain the output -- Processing locus 711: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACGTCCCCAATTGTTGTATGAAATTTGATGTATGTGAGGTAGAAGGTCATGTTCTATAAGCTCATTCGACCAAATTTGTG TGTTTGTAAACTTAACTTTGATTTGTTCATCTCCAAGGTTCTCTCAAAATGAACGTCAGACTTATTGGGCACTCTTGTTT TCTTGTAGCTCCAAGTTTAATGGAAGCATTAACTACTTCAAGGGTTGTGCCAGTTCAGGTCCCCTGTAGAAAGCTCTCTT CACTTTTTGCTAATTTTTCTTGCCTTGAGTTAAGGAGATATCCTTGTAGAGGTCTTGTCTCCATTATGAACCATCCCAAA TTGCTTCGTCCAGTGACTGCTTCTGTGCAGCCTCACGAGTTGTCGACTTTGGGGCATGAAGGCAACATTGTTCCTTCTAA AGAAATTCTTGATCTTTGGAGAAGTGTTGAAGCGGTATGTTTCGATGTGGACAGCACGGTTTGTGTGGATGAAGGTATTG ATGAACTTGCTGAGTTTTGTGGAGCTGGAAAGGCTGTTGCTGAATGGACTGCCAGAGCTATGGGTGGATCTGTTCCATTT GAAGAAGCTCTAGCTGCAAGACTTTCTTTATTCAAGCCTTCTCTTTCAAAAGTAGAGGAATATCTCGACAAGAGACCCCC AAGGCTATCTCCTGGCATTGAAGAGTTGGTCAAGAAACTCAGAGCCAATAACATTGATGTCTATTTGATATCTGGAGGTT TTCGACAGATGATCAATCCTGTAGCATCGATTCTTGGCATCCCTCGGGAGAATATCTTTGCCAACAATCTTCTATTTGGA AACTCCGGCGAGTTTCTGGGATTTGATGAGAACGAACCTACGTCAAGAAGTGGAGGCAAAGCCAAGGCAGTGCAACAAAT ACGAAAGGGTCGTCTCTACAAGACTATGGCCATGATTGGAGATGGTGCTACTGATCTCGAAGCACGTAAACCTGGTGGCG CAGATTTGTTCATATGCTACGCTGGAGTTCAGCTTCGTGAAGCCGTTGCAGCAAATGCAGACTGGCTTATCTTTAAGTTT GAATCTCTCATAAACTCATTGGACTAAAAGCCTCTATAGTGATTATTCTCGTTTAGCTAAACTCATTATTCATATCTCCA AATTTGTACCCTTTTATGAATTTGTATCAAATACCAATTGAATCAA .................N.........N...N................N............................... ................................................i............................... ...................N............................................................ .................................................................N.............. .......................................................N........................ ....................................N.......N.......................N........... N..........................................N...............i.................... ................................................................................ ........................................................N....................... ........i....................................................................... .........................N...................................................... .........................i.....i.........N...................................... .............N........................................N......................... ................................................................................ ...............N.............................. Pos Score Pred ------------------------ 18 0.208 - 28 0.310 - 32 0.399 - 49 0.290 - 129 0.704 Yes 180 0.445 - 306 0.486 - 376 0.189 - 437 0.343 - 445 0.286 - 469 0.139 - 481 0.260 - 524 0.229 - 540 0.546 Yes 697 0.098 - 729 0.542 Yes 826 0.157 - 906 0.629 Yes 912 0.652 Yes 922 0.476 - 974 0.275 - 1015 0.277 - 1136 0.034 -

Explain the output -- Processing locus 712: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTGGGAATTTCTGAATTAGTATCTATATCTCAGGAATCATGATGTTTTAGTTCTATTCAGTCTAGAAACACTTCATAAT TGTGCTAGCTGCTACATCTGATTGGAATTCACAACCATTGTTAGTTTTTTGGGAATCGTGATGATCAGTGTGTACTTAAC TTGTTTCATTTTATGCAGAATGATTGACTTATCTACTTTATCTCCTGCGGAGATCACGGTGATGGGATCCGTGTTCTGTG TTTTGCTCTCGATGCACTTCACTATGCAGCTTGTATCCCAGCATCTGTTTTACTGGAAGAAACCTAATGAACAGAGAGCC ATTCTTATTATTGTGCTTATGGCTCCTGTTTACGCTATCAACTCTTTTGTGGGCTTGTTAGATGCTAAAGGAAGCAAACC TTTCTTCATGTTTCTAGACGCCGTTAAGGAATGCTATGAGGCACTTGTCATTGCTAAATTCTTGGCTTTGATGTATAGTT ATGTAAACATATCGATGAGTGCACGGATTATCCCAGATGAATTCAAAGGGCGAGAAATCCACCATTCGTTTCCAATGACT CTTTTCGTGCCCCGTACCACACATCTGGATTACCTCACACTGAAACAGCTCAAGCAATGGACATGGCAGTTTTGTATTAT CCGCCCAGTGTGTTCCATCTTGATGATAACATTACAGATCCTTGGGATTTATCCAGTTTGGTTGAGCTGGATCTTCACTG CTATTCTCAATGTTTCGGTTTCCCTGGCGTTGTATTCTTTGGTGAAATTCTACCATGTTTTTGCTAAAGAGTTGGAGCCT CATAAGCCTCTTACAAAGTTCATGTGCGTCAAAGGGATAGTGTTCTTCTGCTTTTGGCAGGGAATAGTCCTGAAGATATT GGTGGGATTGGGATTAATTAAGTCGCATCATTTCTGGTTGGAGGTGGATCAGCTTGAAGAAGCTCTGCAAAATGTGCTTG TATGTCTTGAGATGATCGTCTTCTCCATCATTCAACAGTACGCTTTTCACGTTGCTCCTTACAGTGGAGAAACTGAAGCT AAGATGAGATTCAACAAGAGAGATTGATTCTGAGAGTGCAGACTCTTTCCAAATTTAGACCAGAGAAGATCTTATCTTCT TCTTTTTTTGGTTAATGTGAATGTTAATGTTGTTATGTTACCACTA .......................................N..N..................................... ............................................................N................... ............N......N.........................................N.................. ...........N...........N..........................................N............. ..................i..........................................N.................. .......N......................N....N..................................N......... i.............i.....................N.....................................N..... ........................................................i.....i................. ......................N......................................................... .........N............................................N......................... .....................N.......................................................... .......................................................................N........ ...N............................................................................ ..............N.....N.....N.......N........... Pos Score Pred ------------------------ 40 0.146 - 43 0.375 - 141 0.254 - 173 0.397 - 180 0.410 - 222 0.419 - 252 0.088 - 264 0.196 - 307 0.228 - 339 0.613 Yes 382 0.106 - 408 0.308 - 431 0.402 - 436 0.356 - 471 0.329 - 481 0.546 Yes 495 0.509 Yes 517 0.229 - 555 0.285 - 617 0.787 Yes 623 0.820 Yes 663 0.121 - 730 0.139 - 775 0.438 - 822 0.154 - 952 0.279 - 962 0.595 Yes 972 0.389 - 1044 0.301 - 1135 0.032 - 1141 0.033 - 1147 0.037 - 1155 0.044 -

Explain the output -- Processing locus 713: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACTAGACAGTAGTTTCTCATTGGGTTTTGGTTGATTTAGGATAATTCTTGGGTCATATTTGGTGTTGGGTCTGTTGGTT GGTTTTTTAGATTGGTAGTGAGGTTTTTACTATAGGTATTGGATTTTTGTGGAGATGGATAGGCCTAGACAAAATGATCA TTTGGGTGTGAATAGGATTGGGAAAAATATCAGAAAGAGTCCTCTTCATCAATCGACTTTCGCTGCCAGTACTAGCAACG GTGCCGCTCCAAGGCTTCAAACACAGCCTCAGGTTTATAACATAAGCAAGAACGACTTTAGAAGTATTGTTCAGCAGCTA ACGGGTTCTCCATCACGTGAAAGCCTTCCTCGGCCTCCTCAGAACAACTCGCTAAGGCCTCAGAATACACGGTTGCAGCG GATCAGACCATCCCCCTTAACGCAGTTAAACCGGCCTGCGGTTCCTCTCCCTTCCATGGCTCCGCCACAGTCTCATCCTC AGTTTGCTAGACAGCCTCCACACCAGCCACCTTTCCCACAAACCACACAACAACCAATGATGGGTCACAGGGACCAGTTT TGGTCTAATACAGCTGAGTCTCCCGTCTCAGAGTATATGCGTTATCTTCAAAGTTCACTTGGAGATTCAGGACCCAATGC TAACCAAATGCAGCCAGGTCATGAGCAGCGGCCTTATATCCCAGGTCATGAGCAACGGCCATATGTGCCAGGTAATGAGC AGCAGCCATATATGCCAGGTAATGAGCAGCGGCCATATATCCCAGGTCATGAGCAGCGGTCATATATGCCAGCTCAATCT CAGTCTCAGTCTCAACCTCAACCTCAACCTCAACCTCAGCAACATATGATGCCTGGACCGCAACCTCGAATGAACATGCA GGGCCCTCTTCAACCTAACCAGTATCTACCACCACCCGGGTTAGTTCCTAGCCCAGTGCCTCATAATCTTCCTTCTCCTC GGTTCAATGCTCCTGTACCTGTGACGCCTACTCAGCCCTCTCCCATGTTCAGTCAGATGTATGGTGGATTTCCTTCTCCT CGATATAACGGTTTTGGACCACTGCAGTCACCTACATCCCAATTTCTTCAACCATCTCCTACCGGTTACCCGAATATGTT CTCTCCGAGATCGCCTTACCCATTGCTGTCACCAGGAGTTCAGTATCCTCAACCACTTACCCCAAACTTCTCATTTTCAC AAATTGCTCAACAAGGAAGTCTTGGACCCGGTGCAGGTCCAAGTCAAGGTCCTCCTCAGCCTCCTCCTTCTCCAGGGCTC ATGTTCCCGTTATCTCCATCCGGGTTCTTCCCCATGCCAAGTCCAAGATGGAATGATTACTAGGTGCTCTTTTTCTCTCC ATCAGATTCATTAAATTAAGTAAATCGGATTATGTTTATTCAGGTTTCCTTAGGTTAGTCTGTACCTTTGAAAGAAGCTT AA ................................................................................ ......................................................N..................N...... ................................................................................ ................................................................................ ................................................................................ .......................................................i........................ ........................................................i..i.................... ....................................i.......................................N... .......i............N..........................N..............N...........N..... ...........i.........N..........................N................i.............. .............................................i..i....................i.....i.... ................................................................................ ......N.....................................N...........N...N................... ...........................................................................N.... ................................................................................ ................................................................................ N................................N.............N....i........................... ...............................N................................................ .. Pos Score Pred ------------------------ 135 0.475 - 154 0.359 - 456 0.550 Yes 537 0.831 Yes 540 0.527 Yes 597 0.549 Yes 637 0.466 - 648 0.733 Yes 661 0.260 - 688 0.223 - 703 0.342 - 715 0.212 - 732 0.517 Yes 742 0.434 - 769 0.239 - 786 0.722 Yes 846 0.533 Yes 849 0.693 Yes 870 0.508 Yes 876 0.577 Yes 967 0.192 - 1005 0.326 - 1017 0.440 - 1021 0.429 - 1116 0.416 - 1281 0.110 - 1314 0.418 - 1328 0.401 - 1333 0.537 Yes 1392 0.105 -

Explain the output -- Processing locus 714: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAGCTCGGATCTGAGATTGATTTGTTTCTTTGCGTCTTTGTGTAGATTTGACTTTAATTTTGAGATTGTTGTGTGCTGA TATACAGCTTAATTAATATAGCCTCTGATGTGTTATTATCGATGTGTCATTATCAATCTACAGGTGACATTTTATAAGTG TGGAGTTTACTTCAGCTTTATTATTCAGGAATTGATGGATCAGTACGAGAAAGTTGAGAAGATTGGTGAAGGAACTTACG GTGTGGTTTATAAGGCACGTGACAAAGTGACTAATGAGACAATTGCTTTGAAGAAGATCAGGCTAGAGCAGGAGGATGAA GGTGTTCCTAGCACAGCAATCAGAGAAATCTCCCTCTTGAAAGAAATGCAGCACAGCAACATTGTCAAATTGCAGGATGT GGTGCACAGCGAGAAACGTTTGTATCTGGTTTTTGAGTATCTTGACTTGGATCTCAAAAAGCACATGGATTCTACTCCTG ATTTCTCCAAGGATCTACATATGATCAAAACATATCTTTACCAGATTCTCCGTGGAATTGCGTATTGCCACTCTCATAGG GTTCTCCATCGTGATCTGAAGCCACAGAATTTGTTGATTGATCGCCGCACAAACTCACTGAAGCTTGCTGATTTTGGACT GGCCAGAGCATTCGGTATCCCTGTCAGGACATTTACTCATGAGGTTGTTACTCTCTGGTACCGAGCACCAGAGATACTCC TAGGATCTCATCATTACTCTACACCTGTTGATATTTGGTCTGTGGGGTGCATATTTGCTGAGATGATCAGCCAAAAGCCC TTATTTCCTGGAGACTCCGAGATTGATCAACTCTTCAAGATTTTCAGAATCATGGGAACTCCGTACGAGGATACATGGCG TGGGGTAACTTCTCTACCGGATTATAAATCTGCTTTCCCTAAATGGAAACCAACGGACCTAGAAACTTTTGTCCCCAATC TAGATCCCGATGGAGTCGATCTCCTTTCTAAAATGCTGTTAATGGATCCGACCAAAAGAATCAACGCAAGAGCCGCCCTG GAGCATGAATACTTCAAGGATCTTGGAGGCATGCCTTAGAAAGGCATAAAACCAGTAATCTCCTTCATTCTATATATAAT TATCAATCCTAAGAAAATGAAGAACAATATTAATGGGTTTTGTTTATTCTTTTTCTGA ................................................................................ ...........................i.............N...................................... ..................................i............................................. .................................N.........................................N.... .............................................N..............................N... ................................................................i............... ....................i........................................................... ................................................................................ ......................................N......................................... ..............................................................N................. ...................................................N......................i..... ..........................................i..................................... .........N......................i........i...................................... ....N.........................N................................................. ................N...............N......................... Pos Score Pred ------------------------ 108 0.553 Yes 122 0.460 - 195 0.826 Yes 274 0.108 - 316 0.294 - 366 0.392 - 397 0.197 - 465 0.694 Yes 501 0.567 Yes 679 0.307 - 783 0.450 - 852 0.244 - 875 0.507 Yes 923 0.509 Yes 970 0.456 - 993 0.813 Yes 1002 0.526 Yes 1045 0.259 - 1071 0.250 - 1137 0.072 - 1153 0.101 -

Explain the output -- Processing locus 715: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAGTGAATTTTGATGTGGAGCTATTTGGCTGAATATAATGCTGAAACATTTGTGAAGATTTAGATGTTTGATTGATTA GAATTCTTGTGCAAAGTCTGATGGCTTCTTGATGCTAAAGAGTTATGTGTATTAATTTTGATGCAGGACACAATGATGGA CCTAACGAAACTGAAGCCTCCACAGATCACTTTTTATTGCTCGGCGTTTTCCGTCTTGCTCACACTTCATTTCACGATAC AGCTTGTATCGCAGCATCTCTTCCACTGGAAGAACCCTAAGGAACAAAAGGCTATACTCATCATTGTGCTTATGGCTCCT ATCTACGCCGTTGTTTCCTTTATTGGTTTGTTAGAAGTCAAAGGAAGTGAAACCTTCTTCCTCTTTCTTGAATCCATCAA AGAATGCTACGAAGCTCTTGTCATTGCCAAATTCTTGGCATTGATGTATAGTTATCTAAACATATCTATGAGCAAAAACA TTTTGCCTGATGGAATCAAAGGAAGAGAGATTCACCATTCTTTCCCAATGACTCTTTTTCAGCCTCATGTAGTCCGTTTG GATCGTCACACTCTTAAACTTTTGAAATACTGGACATGGCAATTTGTGGTCATTCGACCCGTGTGCTCCACTTTGATGAT AGCTTTACAGCTCATCGGGTTTTATCCTTCATGGTTGAGCTGGACATTCACTATCATTGTCAATTTCTCGGTTTCTCTGG CCTTGTATTCTCTTGTGATATTCTACCATGTGTTTGCTAAGGAGCTTGCTCCTCATAATCCACTCGCAAAGTTCCTCTGC ATCAAAGGAATTGTCTTCTTTGTCTTTTGGCAGGGAATAGCACTAGACATACTGGTGGCAATGGGATTCATAAAATCTCA CCATTTCTGGTTGGAGGTAGAACAAATCCAAGAAGCAATTCAGAATGTGTTGGTATGTCTTGAGATGGTTATCTTCGCTG CGGTTCAGAAACACGCTTATCATGCTGGTCCTTATAGCGGCGAGACCAAGAAGAAACTCGATAAAAAGACCGAATGATTT GGGCGTTAGAGTGGACATGGACTGGTTTTAAACCAGCCATTTTTATAGCCAATCTTTGGTGATCTTGTATGTTGTTATGT TGAGGAGAAAGATCTA ..............N........................N.........................N.............. ....................i..........N............N...............N...........N..N.... ................................................................................ .......................................................................i........ ................................................................................ ...N.......................................N.......................N............ .........N.....................................N..................N............. ...................................i.......................................N.... ..............................N................................................. ...........................i.................................................... ............................................................i................... ............................................N.........N.........i............... .....................N...................................................N...... ................N...................................................N.......N... ................ Pos Score Pred ------------------------ 15 0.337 - 40 0.302 - 66 0.467 - 101 0.602 Yes 112 0.097 - 125 0.439 - 141 0.221 - 153 0.307 - 156 0.338 - 312 0.588 Yes 404 0.305 - 444 0.318 - 468 0.475 - 490 0.183 - 528 0.441 - 547 0.111 - 596 0.732 Yes 636 0.126 - 671 0.269 - 748 0.662 Yes 861 0.708 Yes 925 0.484 - 935 0.482 - 945 0.657 Yes 982 0.176 - 1034 0.224 - 1057 0.084 - 1109 0.026 - 1117 0.045 -

Explain the output -- Processing locus 716: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCTTCATCTCTTTCGATCAACCAATGTAAGTTTGTGACCCTTATATCCCATCAAATCAATCGAGTCTTCTGATACCGTT CCTGTAGTGATTCGATCATAATTCATACAGAAACCATTAGATGGTTATACGTGTTGGATGTTTAGAGAGCTATTAGCAGA TTGCATTGGGAGATTCCGTAGCTTGTTTATATCTCTTTTGATTACTAAACTCTGCAGTTTTATAATTTATGAAATTTGCA GTTACGACATGGATGAGGTTGTTGTTGAAGTGGAGAAGACGAAGAGAGAATGGGAAGAAGCGTACGAGAAGACGATTGGA CATATTATCTCGATTCAACAGTACGGTAAATCAAGGAGGGGAGATGGAGGAGAAGAGAAGTTTTCGTTACAGAGGCTCAA CGGATTGGCTCAGGATGGTCTCTCTCTTCTCAATTCCCTTCAATTCAATTTGGATCTGCTCGCACCTCAGCTTCCTTCTG ATGATCAGGTTCAATCCACTCAGTCACTCCTTGAAACGTGGAAGAATCAATATCACAGTTTGAGGGTAAACTTGAGAAGT GCTAATTTGCAAGCTAAGGATAACATGAGGAAAGCAGCTCAAGAAGAGAGAGAGCTTCTCCTCGGTGGTGGAACAGAGTC TACAGTTCTCAGACGTAAACGACAAGCAAATGCTGGCGTGACATCCGATGCTGAAAGCATAACTGAAAGTCTCAGGCGTT CACGGCAGCTGATGGTTCAGGAGGTTGAACGAAGTACAAACACTCTCGTGGCTTTTGATGAATCTACTGGAGTACTTAAA AAAGCCGAGAGTGAATACAAAGGTCATAGATCTCTGTTGTCGAGGACCCGTAATCTACTTTCAACAATGCAGCGTCAAGA TGTAATTGACAGGATAATCCTCATAGTTGGATTTTCTCTCTTTGTCTTTGCTGTTGTTTACGTTGTTTCAAAACGCATCG GAATTCTGAAACTGCAACGAATGGCTACTGCTGCTATTAAAGCTCAATTAGCTGGAAAAGCAGCAAATGGTGTAGGAGAT GATGTTATGCCGCTCGGACAACAGTTTGATGGCAATACAGTTCCAACTGTTAATATTCCTCTACAACAACGCATGCACGA TGAACTATGAAGCTCATTAGATGCTTGTAACATTGTTCAGGAATATATATATTACAAGTTGATGTTGTTACCTAGGAATG CTCTCTATATTACCACATGTTTCATGGAC ........................N....................................................... ........................................N................N...................... ....................................................................N........... ........i...N....................................i.............................. ...........................................N.................................... ..............N................................................................. N............................................................................... ........................i....................................................... .............................N.................N................................ ...........i.............................................N...................... ..................................................................i............N ................................................................................ ....................i.............................................N...........N. ......N.............N........................................N...............N.. ................N......N..... Pos Score Pred ------------------------ 25 0.265 - 121 0.243 - 138 0.179 - 229 0.116 - 249 0.801 Yes 253 0.251 - 290 0.642 Yes 364 0.498 - 415 0.263 - 481 0.220 - 585 0.501 Yes 670 0.431 - 688 0.160 - 732 0.520 Yes 778 0.197 - 867 0.599 Yes 880 0.275 - 981 0.904 Yes 1027 0.409 - 1039 0.169 - 1042 0.296 - 1047 0.313 - 1069 0.129 - 1113 0.248 - 1120 0.119 - 1127 0.320 - 1141 0.137 - 1182 0.057 - 1198 0.050 - 1217 0.068 - 1224 0.058 -

Explain the output -- Processing locus 717: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAACCCAGCCCCAAAAAATAACAGTATAGGTAATGATATTATGTCGCTTTAAGTAGATAATTACAACGTTTGAATAATCT GCATATTCAAAATTTCAATAGAGTTGGAACCAACATCGAAGCAAACTGTTGGAAAACAAGACTAACCGTCGTGTACGTAT CTCTTCGTCTATATATATGTATGTGTGCATAAATTTTAAAAACATGTACTCGTACAAAAAAATAAGTTACCAGATGGAAG AGGTAATGTCAATGATCTTCCACGGAATGAAACTAGTTAAATCGCTCGAGTCCAGCTTACCGGAAAAGCCACCGGAATCT CTATTGACATCTCTTGACGAGATCGTAAAGACATTCAGTGATGCAAACGAGCGGCTGAAGATGTTACTAGAGATTAAGAA CTCCGAGACTGCGTTAAACAAAACCAAACCGGTGATTGTGTCCGTTGCAAACCAGATGTTGATGCAGATGGAACCGGGTC TGATGCAAGAGTATTGGTTAAGGTATGGCGGGTCCACGTCGTCTCAAGGAACGGAGGCCATGTTCCAGACGCAGCTCATG GCCGTTGATGGTGGTGGAGAAAGGAATTTGACGGCTGCAGTAGAAAGATCCGGCGCTAGCGGTTCCTCCACGCCAAGGCA GCGGAGAAGGAAGGACGAAGGAGAAGAACAAACGGTGTTGGTGGCGGCGCTAAGGACGGGGAACACAGATCTGCCACCCG ACGATAACCATACTTGGCGTAAATACGGTCAAAAAGAAATTCTTGGCTCTAGGTTTCCTAGGGCGTACTATAGGTGCACC CACCAAAAGCTATACAATTGCCCGGCCAAGAAACAAGTCCAACGCCTTAACGACGATCCCTTCACCTTCCGGGTCACATA CCGTGGCTCACACACTTGCTACAACTCAACCGCTCCAACCGCATCCTCTGCCACTCCAAGTACCATTCCAATCTCATCCG TCACCACTGGTCATTCTGTTGACTACGGTCTTGCTGTCGTTGACATGGCTGATGTTATGTTTGGTAGCGGCGGGGTCGGA ACCAACATGGATTTCATATTTCCTAAAAACGATCCATCTTAACATCATCACCGTTTCCGGCAGCTGGATGACGGATTGAC GGTGACGAAAACAATCAAGATTCATCTACGTACAGAGGAAAAATATACAGTATTTCTTAAG ................................N.......i....................................... ................................................................................ ................N...N......................N.............................N...... .....i.....N..............N..................................................... ........................................N...................i................... .......................................................i.....N.....N............ ..N.....................i..................................i.................i.. .......i........................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ............................................i......N....i....................... ......N............................................................N............ ............................................................. Pos Score Pred ------------------------ 33 0.243 - 41 0.656 Yes 177 0.386 - 181 0.123 - 204 0.494 - 234 0.367 - 246 0.555 Yes 252 0.250 - 267 0.366 - 361 0.171 - 381 0.561 Yes 456 0.509 Yes 462 0.341 - 468 0.230 - 483 0.312 - 505 0.511 Yes 540 0.709 Yes 558 0.781 Yes 568 0.717 Yes 1005 0.799 Yes 1012 0.275 - 1017 0.769 Yes 1047 0.329 - 1108 0.055 -

Explain the output -- Processing locus 718: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGGAAATCGTAGGACAAAAAAGTCCCCCAACCAATTAATTTGTCTCCCCCACCGAATGTACACGTGGCACCTTGTCTGAT TTTTCGTCAGCTCTACGATAACCAAAAAAAGACAACATTTGTAGCTGTTATTATTTATTGGGCCTGTTAATGGGCCTTAT AAAGAATTATGAAAGCTTCTGTGTCAAGAAGAAGGTTACTTTAAATCTGTATCAATATCAGCTCCACGACGGCGATTCGT CGTTGTCCTCCGAACCAAGCAAACTCCGATCCCTCACCGTGGCTCCGGATATTTTACGGCGGTTGTTCCCGGCTCGGACT CGAAGAATTGTCCGGATTCTCCCTTTGACCAGGCTGGAGATGAACCTTTTCCGGTTCTGCTCCGGTCTGAAAGTCCTCGG CTATTTCATGATCCTTCTTGTCGTCGCCGTCGTTGGTGTATCTTACTACGCCGTCGTAGTTAGCACATGGTGGCCGATCT TGATCCGCGGAGACCATGGAGCTTTATCCGCCCTCGCTGCCTTAATCATCTTTGTTTTCCATTTCCTGCTGATAATGCTG TTGTGGAGCTACTTCACAACAGTTTTTACAGACCCAGGTTCTGTTCCTGAGCATTTCAGAAGAGAAATGGGAGGTGGTGA CAGTTTGGAGGCTGGTACTTCGACAGATCAAGGAGCTTTTGGCTCTTTAGGTTACTGTACCAAATGCCGGAATGTTAAGC CGCCTCGTTGCCATCATTGTTCTGTGTGTCAAAGATGTGTTCTTAAGATGGATCATCATTGTGTTTGGATTGTAAACTGT GTTGGAGCACGCAATTACAAGTTTTTCCTTCTCTTTCTGTTCTATACTTTTCTTGAGACAATGTTGGATGTTATTGTCTT GCTTCCTAGTTTCATTGAATTCTTCAGCCAAGCTATAAAGCATTCTTCTTCTCCGGGTAAACTGGCCTCTCTCGTTCTGG CATTCGTTCTTAACTTTGCGTTCGTTCTCAGCCTTCTTTGTTTCGTAGTCATGCATATTTCTCTTCTCTCATCCAACACT ACTTCAGTTGAGGTTCATGAGAAAAATGGAGAAGTCAGATGGAAATATGACTTAGGAAAGAAGAAAAACTTCGAGCAGGT TTTTGGGAAGAAGAAGGCGTTTTGGCTCCTCCCATTGTACTCTAAAGACGATATCGATAACATAACTTCCCTTGAAGGCC TTGAATTTCCAACTTGTTCTGACATCGACCCTTGATTGAAAGTTGAAACAAAATCAATCAATCATATTCGAAACAGCCTT CAACTGCACTCCAATGTAGATAACTCTCTACTCAAAAAGAATTCTCATTGCCAA .......................................................N........................ .....................................................................i.......... ........i....................................................................... ................................................................................ .......................................i........................................ .......N..........................................................i............. ...............N..........................................................i..... ..................................................................i............. ...............................................................N.......N........ ..................................N............N................................ ............................................................i......N............ ................................................................................ ..................................................i............................. ................N........N............i.......N................................. ................................................................................ ................................................................................ .............N........................................ Pos Score Pred ------------------------ 56 0.194 - 150 0.722 Yes 169 0.689 Yes 360 0.557 Yes 408 0.314 - 467 0.803 Yes 496 0.215 - 555 0.552 Yes 627 0.801 Yes 704 0.102 - 712 0.311 - 755 0.185 - 768 0.379 - 861 0.673 Yes 868 0.288 - 1011 0.583 Yes 1057 0.115 - 1066 0.430 - 1079 0.593 Yes 1087 0.444 - 1294 0.050 -

Explain the output -- Processing locus 719: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTACCGACTTCTCACAACATATCAAATGCCAAATGTGAACTAATGTAACCAAACAATGTTTTTTGGATAATTCACATTG CACAAATGCTTGTATCTGTAGTTTCCAATTTTCTTAAAGGGTGTTTTGGACATTTCGATCTTTTTTTTTGATATGGTAAA TAAAATTACAAACAATTATATTTTTCCGAGAAAGCCATTGTTTCAGTTGATGATGGTGATGATATCTCTTCACTTCTCTA CTCCGCCGCTAGCTTTTCTCAAATCTGATTCCAATTCCAGATTTCTCAAGAACCCTAATCCTAATTTTATCCAATTCACC CCAAAATCCCAACTTCTTTTCCCCCAACGTCTCAATTTCAACACCGGAACAAATTTAAACCGGCGAACTCTGAGTTGCTA TGGGATTAAGGATTCAAGTGAGACAACGAAGTCTGCGCCGTCATTAGACTCCGGCGACGGTGGCGGCGGAGATGGTGGTG ATGATGATAAAGGTGAAGTGGAGGAAAAAAACAGACTTTTTCCGGAATGGTTGGATTTTACCTCGGATGATGCACAGACT GTGTTTGTGGCTATAGCTGTATCGTTGGCGTTTCGTTATTTTATCGCAGAACCAAGATATATTCCTTCTTTGTCTATGTA TCCTACTTTTGATGTTGGAGACAGATTAGTTGCAGAGAAGGTGAGTTATTATTTCAGGAAGCCTTGTGCAAATGATATTG TCATCTTTAAAAGTCCACCAGTTCTTCAGGAAGTTGGGTATACTGATGCCGATGTATTTATTAAACGGATTGTTGCCAAA GAAGGTGACCTTGTGGAGGTACATAATGGGAAACTGATGGTTAATGGTGTTGCTAGGAATGAAAAATTCATCTTAGAGCC TCCTGGTTATGAAATGACACCAATTAGGGTACCGGAAAATTCAGTCTTTGTGATGGGTGATAACCGGAATAACAGTTATG ATTCGCACGTATGGGGTCCTCTGCCTTTGAAGAATATTATTGGACGGTCGGTTTTTCGGTATTGGCCACCAAACAGAGTA AGCGGGACAGTGCTAGAAGGTGGCTGTGCTGTGGATAAGCAATAGAACATATTGTAACGTTTTGATTCGGCATGTCTTCA TTTGAAACACTTGTGCAGATAAAAACGAAAACAATGTACCTTTTTATGAAGGAGAGAGTTGCAC ..........................N......N.........N............N....................... .....N..................................................................N....... .................................................N..N.....N..................... ................................................................................ ...............................................................................N .......................................................................N........ N..N..........................................N...................N..N.......... ...........................................................................N.... ...........N...........................................................N........ .............................................N.....N............................ .........................N..........i......N..............N..................... ........N....N......................................i........................N.. ..........N..................................................................... .......................................................................N........ .................................N...........N.................. Pos Score Pred ------------------------ 27 0.144 - 34 0.298 - 44 0.419 - 57 0.310 - 86 0.168 - 153 0.155 - 210 0.415 - 213 0.444 - 219 0.299 - 400 0.368 - 472 0.398 - 481 0.051 - 484 0.105 - 527 0.233 - 547 0.104 - 550 0.131 - 636 0.498 - 652 0.196 - 712 0.140 - 766 0.143 - 772 0.097 - 826 0.241 - 837 0.623 Yes 844 0.202 - 859 0.296 - 889 0.158 - 894 0.395 - 933 0.598 Yes 958 0.349 - 971 0.498 - 1112 0.078 - 1154 0.048 - 1166 0.038 -

Explain the output -- Processing locus 720: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGAACCCTAGCCCACGAAGGAGATGGCATTGGTTGCAAAAAATGATTGCTACCTTTGAAGTTTGAAACTTATTCTTCCT TGCACAAGTCGCTTTCGATGATACCTCACTCAAGTCACAGTCAACGAACAAAATATCTACAAAATGGGTCACACAGATCC ACCACTAACTTCAAAAATTCTTATCTTCACGACTACGCTTCTGTCTTTACTTCCTCTCTCAACGTACGTAGACAGAAAAA AACAAAACATCATGACAGAAAAATCTGCAGAACTCGTTCGTTTGAACGAACTCAAGGCTTTTGTATCGACAAAAGCAGGT GTGAAAGGACTTGTCGATACCAAAATAACCGAAGTTCCTCGAATCTTCCATATCCCTTCTTCTTCAACTTTATCTAACAA CAAACCTTCTGATATCTTTGGCTTAAACCTCACTGTCCCAATCATTGACCTCGGAGATGGTAACACATCTGCTGCAAGAA ACGTCCTCGTTTCCAAGATTAAAGAAGCAGCTGAGAATTGGGGATTTTTCCAAGTAATCAATCATGGTATTCCTTTAACT GTTCTTAAAGATATCAAACAAGGTGTTCGAAGATTTCATGAGGAAGATCCAGAGGTCAAGAAACAGTATTTTGCTACAGA TTTCAATACAAGATTTGCTTACAACACCAACTTCGATATTCATTATTCTTCTCCTATGAATTGGAAAGACTCTTTCACTT GCTACACTTGTCCTCAAGATCCTCTAAAGCCAGAGGAAATCCCACTAGCTTGCAGGGATGTTGTGATTGAATACTCGAAG CATGTAATGGAATTAGGAGGTTTACTCTTCCAACTTCTCTCAGAAGCTTTAGGTTTAGACTCTGAGATTCTTAAGAACAT GGATTGTCTCAAGGGTTTGCTTATGCTCTGCCATTATTATCCACCTTGTCCACAACCTGACCTAACTTTGGGCATAAGTA AACACACCGACAATTCCTTCATAACAATTCTTCTTCAAGATCAAATCGGTGGTCTTCAAGTTCTTCATCAAGATTCTTGG GTTGATGTAACTCCTGTTCCTGGAGCTCTTGTCATCAGTATCGGTGATTTCATGCAGGCAAGCTCGATTGATGCTTCCTT TTAACTCAAAACAGAGTCTTTCTTTATCAATACACTTGAGTCATTTTGTTTTGCAGCTGATCACAAACGATAAGTTCTTA AGTATGGAGCATAGGGTACGGGC .......................i..................i..................................... .................N.............................................i................ ................................................................................ ...........i.................................................................... ................................................................................ ........................................................N....................... ...............................................................N................ .....................................N.......................................... .......................................................i........................ .........................................................N...................... ......................N......................................................... ................................................................................ ....N..............................................N..................N......... ................................................................................ ...N................... Pos Score Pred ------------------------ 24 0.610 Yes 43 0.688 Yes 98 0.325 - 144 0.711 Yes 252 0.747 Yes 457 0.280 - 544 0.258 - 598 0.109 - 696 0.640 Yes 778 0.274 - 802 0.433 - 807 0.314 - 879 0.356 - 903 0.393 - 1045 0.065 - 1092 0.417 - 1111 0.253 - 1204 0.069 -

Explain the output -- Processing locus 721: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGATTATTAATCTTTTTACTTACTCTGTTTCACCGAATAGGCAAAATAAAAATGTCAATGCTTAACTCGTGTTTGTTTA GTGAAAAAGTAACTAAGTTAATTTTTGGCAAGGTTTCCAAAACTGCGAGAGAAACAACCAGAGCCAGTCAATAAAAACTC AATTATGAATTTTATGATCCATGCAAATCACTTGCTAGTACTCTTCACACTCTCTACAATGTTGTCTATGCTCTGGTTCC TTCTATCTCTTCTCTCTCTTCTTCTACTTCCTTGTCTCCGACCTTGCTTTCCTGCTAAAGGATCTCTGAAAAACAAAAAG AAGATTGACAAGGGAACTTATGTGGTCGGAGAGGAGGAGACACCTAAAGAGCTTCAGAGAGAGCTGATGCCGAGACACGT GGCAGTGATAATGGACGGAAACCGGAGATGGGCTAAACAGACCGGATTGTTGACGTCACAAGGCTACGAGGCTGGAGCTA AACGGCTTCTCGAGTTCGCTGACCTCTGCTTCAAACTAGGGATAAATACTGTCTCAGCTTTTGCTTTCTCCACTGAGAAT TGGGGAAGACACAAGATTGAAGTCAAATGCTTGATGTATTTGTTCCAACGTTACCTCAAGTCAAAGATCCAATTTTTCCA GAGCAAGGAAATCCGAGTTTCTGTTATCGGGAATCTTGCGAAGATCCCTGAGTCTCTCCTCCGAACAGTTCACGAGTTAG AGGAAGCTACAAAGAGTTACAAGAAGAAGCATCTCATCTTGGCCATAGATTACAGCGGGAGATTCGACATCTTGGGTGCT TGCAAGAACATTGTGAAGAAATCAGAACAAGGTTTGATCCGAGAGGAAGACGTGGACGAGACGTTGTTCGAGAGAGAGCT TCAGACTCGGTGTACCGAGTTCCCAAGTCCTGATCTCTTGATTAGGACTAGTGGGGAACAGAGGATTAGTAACTTCTTCT TGTGGCAGCTCGCTTATACCGAGTTTTTCTTTTCGCCTGTACTTTGGCCTGATTTCGATAAGCAGAAGTTTATAGAGGCC CTGGTTTCGTATCAGCGCAGGGATAGACGATTTGGCTCCCGGCTTTGATCCACATTTGATCCTATGGTCTGTCATGTTTC CTCTGTTTTGTTCTGTTTCTCTCTGTGTCTTTATGTTCATCTGACTTTTGCAGAAATAATTATACTG ....................................................i.....i..................... ................................................................................ ....N........N......N.....................................N........N............ ................................................................................ ...................i..............................................N............. ..........i................i.................................................... ................................................................................ ..........................i......N.............................................. ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................................ ...............................................................N.........N...... ................................N.................................. Pos Score Pred ------------------------ 53 0.659 Yes 59 0.578 Yes 165 0.316 - 174 0.090 - 181 0.156 - 219 0.443 - 228 0.317 - 340 0.604 Yes 387 0.391 - 411 0.557 Yes 428 0.660 Yes 587 0.624 Yes 594 0.328 - 1104 0.167 - 1114 0.060 - 1153 0.035 -

Explain the output -- Processing locus 722: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTAAATTTCGTTATCGTTCCAACTAATATCAAAGTAATAACTAACTAGAAATTTCATATATAGAACCTAACAACTGTT GTTTGAAAGAGTTATTTTTAGAAGTACAACAAAACGAAACCATTAACCATGTATATATGTGGACATGTCTTTGAATGTAA AATGCAAGCCAATCTCGAGGAAAAAAGTTACAAACCAAAGAAGAAGATGAAGCTTCAAATTATATGGCTCGCTTTGGTTT TAATCGCCGTTGAGACTTATGCAGTTAAGCAAGGGAAAAACGTAACGATCCCCGCGCTAATAGTTTTTGGAGATTCAATA ATGGATACAGGAAATAACAATAATCTTCCCACTCTTCTTAAATGTAACTTCCCTCCATACGGTAAAGATTATCCTGGAGG CTTTGCCACTGGAAGATTTTCTGATGGAAGAGTTCCTTCTGATCTTATTGCGGAAAAATTGGGATTGGTTAAGACATTAC CAGCATATATGAATCCATATTTGAAGCCACATGATCTTCTTAAAGGTGTAACATTTGCATCTGGAGGAACCGGTTATGAT CCATTGACAGCAAAAATTATGTCAGTGATATCGGTGTGGGATCAACTCATATATTTCAAAGAATATATATCAAAGATCAA GAGGCATTTCGGAGAAGAAAAAGCCAAAGATATCTTGGAACATAGTTTCTTTCTCGTGGTGTCTAGTAGCAATGACCTTG CTCACACTTATTTAGCTCAAGCTCATAGATATGATCGTACCTCTTATGCCAATTTCTTGGCTGACTCTGCTGTTCATTTC GTGAGAGAATTACATAAGCTTGGAGCTCGAAAAATTGGAGTGTTTAGTGCAGTCCCTGTTGGTTGTGTTCCACTCCAAAG AACAGTTTTTGGAGGTTTCTTTACAAGAGGATGTAATCAACCTCTAAACAATATGGCAAAACAATTTAACGCAAGACTTT CTCCAGCACTAGATTCTTTAGATAAAGAGTTGGATGGTGTTATTATTTACATTAATGTTTATGATACTCTTTTTGACATG ATCCAACATCCTAAAAAATATGGCAGGTTTTGAGGTGGCTGATAGAGGATGTTGCGGTAAAGGACTGCTCACGATCTCCT ATTTATGTAACTCATTGAACCCATTTACGTGTTCTAACTCTTCAGCTTATAT ................................................................................ ................................................N.......N.......N.........N..... ..................N............................................................. i........................................N...................................... .......................N........................................................ ........N.....................N............................................N.... ..................i............................................................. .......................................................................N........ ..............................N..............N.................................. ................................................................................ ..............................N.....................i........................... .................................N....................N.....N................N.. ...................N............................N............................... ....N............................................... Pos Score Pred ------------------------ 129 0.458 - 137 0.467 - 145 0.178 - 155 0.308 - 162 0.542 Yes 207 0.661 Yes 224 0.539 Yes 259 0.108 - 321 0.575 Yes 362 0.120 - 424 0.242 - 489 0.496 - 511 0.105 - 556 0.161 - 579 0.694 Yes 712 0.228 - 751 0.284 - 766 0.125 - 911 0.085 - 933 0.559 Yes 994 0.332 - 1015 0.464 - 1021 0.190 - 1038 0.206 - 1060 0.353 - 1089 0.155 - 1125 0.036 -

Explain the output -- Processing locus 723: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCAGTTTCAGTCTTCTTCTTTAACCTTCAGCTTAACACAAAACACAATCACTTTCACTTTCATATCTAAAAAATGGAT GATATGAATCCATTCTATTAGTCATCGGGTTTTATGGACCTGTTGCAAAGTCAACAAGAAGACATGTCTCAGTTTGCAAG TGGGTCAACTGAAGTCCCAGCTTTTAGTAGTCAATTGTCTGAAGAAGGAAGTGATCTTGAAGGAAGTGAAGATGAAGTCA AACCGAAGCAAAGTATATCACGAAAGAAATGGACAGCAAAAGAAGATATTGTTCTCGTTAGTGCTTGGCTCAATACCAGC AAGGATCCTGTGATTGGTAATGACCAACAAGGTCAATCCTTTTGGAAGAGGATTGCAGCATATGTTGCTGCTAGTCCTTC CCTTGATGGTCTGCCAAAGAGAGAACATGCTAAGTGTAAACATAGGTGGGGAAAAGTCAATAAAAGTGTCACCAAGTTTG TGGGTTGTTATAAGACAACAACAACTCATAAAACTAGTGGTCAGTCAGAGGATGATGTTATGAAGCTAGCTTACGAGATC TACTTCAATGATACCAAGAAGAATTTCACTTTAGATCATGCTTGGAGAGAATTAAGGTATGACCAGAAATGGTGTGAAGC TACAAGTCGTAAAGGAGATAAAAATGCTAAAAGAAAGAAGTGTGGGGATGGTAATGCAAGTTCCCAACCAATCCATGTTG AAGATGATTCAGTCATGAGTCGTCCTCCTGGTGTGAAGGCAGCCAAAGCTAAAGCAAGGAAGTCAGCCACTATAAAAGAA GGAAAGAAGCCAGCCACGGTTAAAGATGATTCAGGCCAGTCTGTGGAACATTTTCAGAATTTGTGGGAACTTAAAGAGAA GGATTGGGATCGCAAAGAAAAACAAAGCAAGCATCAACAGCTTGAAAGACTACTTTCAAAAACTGAGCCTCTTTCGGACA TTGACTTGTTCCTAAAAAATAAGCTAATCACGGATCTTTGGTCATAAGTCTTAAGTTCTAGTTTGGTCCTAAGTTTTAAG CTAAGCATCAGATGCTTTTTGTTTTTATGTCCCATGTATGATATTGCTTCGTAAGTTCTTGTTTAA ..........................................................................i...N. ...i.............................N.............................i................ .......................................................................N........ ............................N................................................... ...................N.........................................N.................. .....N....................N..................................................... ...................................................N..N....i.................... .......N.............................N....................N.........N........... .......................N.......................N.....N....................N..... ...N..........i................................................................. .........................N...................................................... ................................................................................ ................................................................................ ...........N..............N......N...N............................ Pos Score Pred ------------------------ 75 0.750 Yes 79 0.353 - 84 0.712 Yes 114 0.369 - 144 0.841 Yes 232 0.373 - 269 0.476 - 340 0.209 - 382 0.121 - 406 0.135 - 427 0.372 - 532 0.259 - 535 0.137 - 540 0.566 Yes 568 0.338 - 598 0.260 - 619 0.428 - 629 0.390 - 664 0.472 - 688 0.498 - 694 0.169 - 715 0.213 - 724 0.293 - 735 0.731 Yes 826 0.237 - 1052 0.035 - 1067 0.028 - 1074 0.041 - 1078 0.033 -

Explain the output -- Processing locus 724: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGTTGTGAACTTTTAGAAGATTATATTTTGTTATCGCTAGGGTATAGAGAAAGCTGAGATTTTACAAATTGAAATGGGT CTTTGAGAATCTGTGTAATAGAGAATCTGTTTGTTTTGTCTTTGGTTGGTGGATATGTCCAGTGGAATGGGAAGAGAAGT TGTTGAGGTGCTTATGGACCGAAACGCCGACGTTTCAAGCGCTAGAGTTCATGTTGCTCCTAAGATAGCTGCAGAGGAGA CTGATGAGGAATTTGAAGTCAAAGAATGCACAGAGGAGAAGTCTCTTTCTGAGAATGCACCAAATGTTGGATCTGCTGAG AGAGTTGGAGCTCAGAAATCACCTAAAACCCGTAATGGAAATGCTAAAGTTTCAAAGCAGCAGGATGCTCCTCTTCTCGC GGTGAGGAAGCCATTGCAACCCGAGAACAAGAAGCATATTGATGACGAAGATAATTGCTCCATTGCTTCTTCCGTTGCAA CTTCCATGAGGATGGGTAAGTCTGGATTGACTTATGGAAGTGCTCCAACGTTTAGGAGTGCTCAACGTGCTGAGAAACGC AAGGAGTATTACCAAAAGCTCGAGGAGAAAAACCAAGCGCTTGAAGCTGAGAGGAACGAGCTTGAACAGAGGCAAAAGGA TGAACAAGAAGCAGCCTTGAAGCAACTTAGAAAGAATCTCAAGTTCAAGGCTAAACCCGTCCCCAACTTCTACTACGAGG CACCTCCTGCGAAACCTGAGCTCAAGAAGCTTCCTTTGACACGTCCCAAGTCGCCAAAACTGATCCTTTCTCGGAGAAAA AGCTTCAGCGACGCAGTCAGCTCGTCTTCCCGTGAAGAGATTCTGAAGACAGTCTCTAACCGGAACCGACACAGCACTGG AACAGTTCAGAACAAAGACGATGATCACAGGAACAAGAACACCAATGCTGCGCACGACAGCCCTCGTGTCAGATCAGGCA AAGGTAAATCCGGATTGAAACCAGTGAACGAGTCCTCAGAAGAAGCTTGAAATTTCAAAAGATGATGCTTTGTTGGTCTA ACTTGATCTTGCCGGAGAAAATGTAAAATCAAAGACTCTTTGTTTTTCGGTTATTTCCGGTATACGTTT ..........................................................................N..... ......................................................i...........i............. .............i....................................N............................. ...N.....................N............................N........N................ ..................................N.....N.......................N............... .........................................N...................................... .....i.....i.....................N.............................................. ...............................................................................N ................................................................................ ................................................................................ ................................................................................ ....................i.......................i................................... .............................................................N..N............... ....................N................................................ Pos Score Pred ------------------------ 75 0.352 - 135 0.832 Yes 147 0.799 Yes 174 0.700 Yes 211 0.079 - 244 0.194 - 266 0.375 - 295 0.118 - 304 0.325 - 355 0.199 - 361 0.183 - 385 0.127 - 442 0.294 - 486 0.772 Yes 492 0.745 Yes 514 0.369 - 640 0.311 - 901 0.627 Yes 925 0.514 Yes 1022 0.132 - 1025 0.089 - 1061 0.096 -

Explain the output -- Processing locus 725: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGCAAGCCCATGGGCGTCCTGCCCAATTGACAGCTCTAGCTATGTAAGGTACTTCTGAATCTTCCCACTAAGGATGTGGT TAGGACTAGTGTTTTTTCTAAGAGATGGAAAAATCTCTGGAAACGGTACCTGGATTAGACTTAAGTGGCGAGGATATCGA AATGTACTACCAATACGTGAGATTTGTGGATAGATTTCTGAGATTTAATAGGAACTATCAGATAGAATATTTTAAGTTAA GCTACCCTGGTGATGGGAGTTCTGAGCGTGAAGTTGATCTGCTCAAGCGGTGGATCGATACTGTCATTCAGCTGAAGGTT AAAGATTTAGATTTTTTTGACTATTCATGGGAATGGGGGTTTGATAACTTTCAGCTACCTTCAACCATTTACATTTGTGA GAGCTTGGTATCCTTAAAGCTATCTACTGTTACCCTGCCTAGTGTCAAGTCTGTTTCTTTACCTTTTCTCAGAGTTCTCA AGCTAGGTGCAGTTAAGTTCGCCGACCATTTGGATTTAGAAACACTCATCTCAGGCTGCACTGCTCTCGAAACCTTAGCT ATAAGATTCTTTGATCGCGTCCAAGTTTTACAAGTGTCTTCTCAGTCTCTACTGAGCTTCACTCATGTTGCGCCCAAGCT TGTCTTTACTCATGCTGTGCGAAAGCCTTTGGCTGAAAAAGATCTATCAATTGTAATTGATGCTCCCAGGCTTGAGTATC TGAAACTCAGTGATCATCAGACTTCAAGTTTCATAATAAAAAATCCTGGTTCCCTTGTTGCGGTGGATATTGATATTAAC TTGTCTTCCGGCTTCGATTCGAGGAATATGATTCGTGATTTTCTTGTTGGGATCTCTAGCGTCAAAAATATGACCATCTC TTCCATTATTCTTAAGATCATCTATGATTACTTAAGATGTGAACCACTACCTCCCCATTCCGTAATCTATATTTCTTGCA TGTTGAGTTCTACGACTACAGGTTTAAAATGTTGCCAACCTTTCTTGAGATCTGCCCAAATCTAAAATCGCTTATATTGG TAAGGATTTTTCATTGACCCTTGTTATAATGCAAATCGTGAAAATATCATAAACGCAACTTGTTTTGGTTCACTTGATTG TACT .........N...............................N...............................N...... ........................i....................................................... ............N................................................................... ..........................N.....i............................................... ................................................................................ ................................................................................ ................................................................N............... ...........N...............................................N.................... ................................................................................ ...........................i.........................................N.......... .......................i............i..........................................N ............................N................................................... ............................N................................................... .... Pos Score Pred ------------------------ 10 0.498 - 42 0.496 - 74 0.244 - 105 0.557 Yes 162 0.459 - 253 0.206 - 347 0.408 - 353 0.506 Yes 625 0.163 - 652 0.169 - 700 0.131 - 828 0.506 Yes 870 0.412 - 904 0.532 Yes 917 0.735 Yes 960 0.452 - 989 0.387 - 1069 0.043 -

Explain the output -- Processing locus 726: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGTTGTGAACTTTTAGAAGATTATATTTTGTTATCGCTAGGGTATAGAGAAAGCTGAGATTTTACAAATTGAAATGGGT CTTTGAGAATCTGTGTAATAGAGAATCTGTTTGTTTTGTCTTTGGTTGGTGGATATGTCCAGTGGAATGGGAAGAGAAGT TGTTGAGGTGCTTATGGACCGAAACGCCGACGTTTCAAGCGCTAGAGTTCATGTTGCTCCTAAGATAGCTGCAGAGGAGA CTGATGAGGAATTTGAAGTCAAAGAATGCACAGAGGAGAAGTCTCTTTCTGAGAATGCACCAAATGTTGGATCTGCTGAG AGAGTTGGAGCTCAGAAATCACCTAAAACCCGTAATGGAAATGCTAAAGTTTCAAAGCAGGATGCTCCTCTTCTCGCGGT GAGGAAGCCATTGCAACCCGAGAACAAGAAGCATATTGATGACGAAGATAATTGCTCCATTGCTTCTTCCGTTGCAACTT CCATGAGGATGGGTAAGTCTGGATTGACTTATGGAAGTGCTCCAACGTTTAGGAGTGCTCAACGTGCTGAGAAACGCAAG GAGTATTACCAAAAGCTCGAGGAGAAAAACCAAGCGCTTGAAGCTGAGAGGAACGAGCTTGAACAGAGGCAAAAGGATGA ACAAGAAGCAGCCTTGAAGCAACTTAGAAAGAATCTCAAGTTCAAGGCTAAACCCGTCCCCAACTTCTACTACGAGGCAC CTCCTGCGAAACCTGAGCTCAAGAAGCTTCCTTTGACACGTCCCAAGTCGCCAAAACTGATCCTTTCTCGGAGAAAAAGC TTCAGCGACGCAGTCAGCTCGTCTTCCCGTGAAGAGATTCTGAAGACAGTCTCTAACCGGAACCGACACAGCACTGGAAC AGTTCAGAACAAAGACGATGATCACAGGAACAAGAACACCAATGCTGCGCACGACAGCCCTCGTGTCAGATCAGGCAAAG GTAAATCCGGATTGAAACCAGTGAACGAGTCCTCAGAAGAAGCTTGAAATTTCAAAAGATGATGCTTTGTTGGTCTAACT TGATCTTGCCGGAGAAAATGTAAAATCAAAGACTCTTTGTTTTTCGGTTATTTCCGGTATACGTTT ..........................................................................N..... ......................................................i...........i............. .............i....................................N............................. ...N.....................N............................N........N................ ..................................N.....N....................N.................. ......................................N......................................... ..i.....i.....................N................................................. ............................................................................N... ................................................................................ ................................................................................ ................................................................................ .................i.......................i...................................... ..........................................................N..N.................. .................N................................................ Pos Score Pred ------------------------ 75 0.352 - 135 0.832 Yes 147 0.799 Yes 174 0.700 Yes 211 0.079 - 244 0.194 - 266 0.375 - 295 0.126 - 304 0.358 - 355 0.315 - 361 0.165 - 382 0.108 - 439 0.393 - 483 0.772 Yes 489 0.745 Yes 511 0.369 - 637 0.311 - 898 0.627 Yes 922 0.514 Yes 1019 0.132 - 1022 0.089 - 1058 0.096 -

Explain the output -- Processing locus 727: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTAGTTATTGGAAAAATTGCACAGTCTATAAACCCTAATTTTGAGGAATCGGCGTTGTCTAAGTTATTTGGTGGTCATG ATCATCTTTTGAAAACAGTAGATATTGATGGTTAGAGTTACGTTTATTCTTCAGATGGCTCTAAAGGAACAGCTAGATAA ATTCAACAAACAGCAAGTGAAATGCCAATCTACTCTTTCGAGCATTGCTTCTTCCAGAGAAAGAACGAGTTCTTCGAGGC AGAATGTGCCTTTGCCTGCGGCAATCACGCAGAAGAAGCCTGATGCAGCTCCTGTCAAGTTTTCAAGCGATACCGAGCGA CTTCAGAACATTAACAACATTAGAAAAGCTCCTGTTGGAGCTCAAATCAAGCGTGTCATTGATCTTCTCTATGAGAGAAG GCTAGCCTTGACGCCAGAGCAAATAAATGAATGGTGTCATGTAGATATGCATGCTAATAAGGCTGTTTTTGATAGTTTAA GGAAGAATCCGAAAGCACATTATGATGGAAGAAGGTTCTCTTATAAGGCTACGCACGATGTTAATGATAAGAACCAGCTT CTTTCATTGGTGAGAAAATACCTTGATGGGATTGCGGTGGTTGATCTCAAAGACGCTTACCCTAATGTAATGGAGGATTT GAAGGCTCTGAGTGCTTCAGGGGACATCTACTTGCTATCAAATTCACAAGAGGACATTGCATACCCAAATGATTTCAAGT GTGAGATTAAGGTTGATGACGAATTTAAAGCTCTATTCCGTGACATAAACATCCCGAACGACATGCTGGACGTGGAGAAG GAGCTGCTGAAAATCGGTTTGAAGCCGGCGACAAACACTGCAGAGAGGAGAGCTGCTGCACAGACGCATGGCATATCGAA TAAACCAAAGGATAAGAAGAAGAAGAAACAAGAGATCAGCAAGAGAACCAAACTCACCAATGCCCATCTTCCCGAGCTTT TCCAGAACCTTAACGGCAGCAGTTCCCGGAACTGAACCTCATAAATGGATGATCATGCGGTTCTAAATTCATATTGAGCC TGAATTTTCTTGTAATTGTAATAATTGTTACAATCTTTAGTTTACAAATTACAA .............................................................................N.. ...........................i..........................i......................... .....................i.......................................................... ...N......................................N..................................... ......................................................................N......... ..........................N...N.......N.......i...N............................. .....................N..N................................N.....N................ .........................N......................................N....i.......... ....................................................................N........... ...............N..............................................N................. ...................................................................N............ ...........................................................i.................... ............................................N...N.....N......................... ...................................................... Pos Score Pred ------------------------ 78 0.114 - 108 0.556 Yes 135 0.535 Yes 182 0.775 Yes 244 0.293 - 283 0.284 - 391 0.078 - 427 0.448 - 431 0.232 - 439 0.333 - 447 0.807 Yes 451 0.177 - 502 0.302 - 505 0.195 - 538 0.079 - 544 0.124 - 586 0.302 - 625 0.308 - 630 0.720 Yes 709 0.087 - 736 0.140 - 783 0.365 - 868 0.328 - 940 0.520 Yes 1005 0.160 - 1009 0.071 - 1015 0.110 -

Explain the output -- Processing locus 728: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTATAAATGAAAAATACGTGCCCATATTTTAATTAATTTATATATTTAGCTATCAAATATTAGGCATAATGTTGGTGAG GTTTCTGAGTATAAAAAATGACAAAGTATGAATACCATCTATACCTTTATTACCTATCTTTCTCGATTTTCCAACCAAAC AACAACAATGGAGAACCATCTTCAACATTCCTTAACCATCATTCCTAAACCGGATCTAATCAAAGAAGAACAACGTTATC ACGAAGATACGGTGAGCTTGCAAGCGGAGAGGATTTTGCATGCCATGACCTTCCCCATGGTTCTCAAAACTGCTTTGGAG CTTGGCGTTATCGACATGATCACTTCTGTAGATGACGGCGTGTGGCTCTCGCCTTCTGAGATCGCTCTTGGTCTCCCAAC CAAGCCCACCAATCCGGAGGCACCAGTATTGCTGGACCGGATGCTAGTTTTGTTAGCCAGCCACTCAATCTTGAAGTACC GTACGGTAGAAACCGGAGATAACATTGGAAGTAGAAAGACCGAGAGGGTCTATGCAGCTGAACCGGTTTGCACGTTTTTC TTGAACCGCGGAGATGGCTTGGGCTCTCTCGCCACTTTGTTCATGGTACTCCAAGGGGAAGTCTGTATGAAGCCTTGGGA ACATCTCAAAGACATGATATTAGAAGGAAAAGATGCATTCACCTCTGCTCATGGCATGAGGTTTTTCGAACTCATTGGTT CGAACGAACAATTCGCTGAAATGTTTAACCGGGCAATGTCGGAAGCTTCCACATTGATTATGAAGAAGGTTTTAGAAGTT TACAAAGGATTCGAAGATGTAAATACTTTGGTGGATGTGGGAGGAGGAATTGGAACAATCATAGGTCAAGTGACTTCCAA GTATCCTCATATTAAAGGCATCAATTTCGATCTAGCATCGGTTTTAGCCCATGCTCCTTTTAATAAAGGAGTGGAGCATG TTTCAGGAGATATGTTTAAAGAAATTCCAAAAGGAGATGCCATCTTCATGAAAGTAAGTAGAAAATGAAATGGTTAATGA TTGACACTTTGACAGTGGATACTACATGATTGGACTGACGAAGATTGTGTAAAGATCCTAAAAAATTATTGGAAAAGTCT TCCCGAG .......N.............................................................N.......... .................N.........i.................................................... .......i........................................................................ .......................................N....N...........i....................... ...............i...............N................................................ ........................................i....................................... ...................................................N............................ .............N............................N.......................i............. .............N..................i.................N....N........................ ....................i..............i.......................i.................... ................N.................N............................................. ..................................................N..........................N.. ...........N........................N..........N................N....N......N... .........................N...................................................... ....... Pos Score Pred ------------------------ 8 0.240 - 70 0.308 - 98 0.373 - 108 0.684 Yes 168 0.849 Yes 280 0.093 - 285 0.337 - 297 0.587 Yes 336 0.579 Yes 352 0.154 - 441 0.583 Yes 532 0.385 - 574 0.457 - 603 0.266 - 627 0.560 Yes 654 0.390 - 673 0.527 Yes 691 0.070 - 696 0.448 - 741 0.677 Yes 756 0.689 Yes 780 0.722 Yes 817 0.193 - 835 0.106 - 931 0.185 - 958 0.452 - 972 0.207 - 997 0.320 - 1008 0.204 - 1025 0.150 - 1030 0.434 - 1037 0.153 - 1066 0.069 -

Explain the output -- Processing locus 729: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCCAAAGAGAGAGGGAGGGAGAGATTTTGGGGTTTGGGTTTTTGTTTTTGTTTTCTCCTTCTTTCTCATTGAAAGTTTT TTTCTTTTACCCTTTTGCTGAACAATAATACTAATTGATATGGGTAATGTGACGTCAAACATGGCTGCAAAGTTCGCTTT CTTCCCACCGCCACCAACATACGACGTGGGTAAAGACGAGGAGACCGGAAAGCTTATGTTCACCGGGATAACACCAGAAA AAAGCATGGATGTTCATCAGCTTACCACCAAATCTGGCAACAAGGTCATTGCCACGTTTTGGAAACACCCTTTCTCTAGA TTCACTCTTCTTTATTCACATGGCAATGCCGCGGATCTCGGCCAAATGGTTGATCTCTTCATCGAGCTTCGAGCTCATCT CCGCGTCAACATTATGAGCTATGATTATTCAGGCTATGGAGCTTCAACAGGCAAGCCAACTGAGCTCAATACGTACTATG ATATCGAGGCTGTGTACAATTGCTTGAGGACCGAGTACGGGATCATGCAAGAGGAAATGATTCTATATGGTCAATCCGTT GGAAGCGGACCAACATTGCACTTAGCATCTCGAGTAAAGAGACTAAGAGGGATTGTTCTTCATAGCGCAATTCTCTCCGG CCTCAGAGTTCTGTATCCTGTCAAAATGACATTCTGGTTCGATATGTACAAAAACATTGACAAGATACGCCATGTAACAT GCCCTGTTCTTGTCATACATGGAACAAAAGATGATATTGTGAATATGTCACATGGGAAGCGATTGTGGGAACTAGCAAAG GACAAGTATGATCCATTGTGGGTAAAAGGAGGAGGGCATTGCAACTTGGAAACATACCCCGAGTACATCAAGCATATGCG AAAGTTCATGAACGCCATGGAGAAACTCGCTCTCAACAATCCACCAAACAAACAACAAAACGACGAGCCAAGCATCAAGG AAACTAAACAAAATCGGTGCTTGAGATTCAGCAAAAGATAGAGAGAGCTGGATTTTGAAGATAGAAACACATAACTAATT CCTTGCGTGCAAGTTGCGACAACACACAAAGGCGTCATTTCCTCAGACAATGCTATCGAA ................................................................................ .......................................i......i.............i................... .......................................................N........................ .....N...N...................................................................... ...................i.....N...................i.................................. .............i......N..............N.........................................N.. ............................................N...........N.........N............. ................................................................................ .........................i.................N...........................N......i. ..................N...........N.............i......i............................ .......i...................................................................N.... .......i........N............................................................... ................................................................................ .................................................N.......... Pos Score Pred ------------------------ 120 0.756 Yes 127 0.563 Yes 141 0.816 Yes 216 0.123 - 246 0.390 - 250 0.328 - 340 0.574 Yes 346 0.231 - 366 0.841 Yes 414 0.725 Yes 421 0.312 - 436 0.314 - 478 0.203 - 525 0.333 - 537 0.370 - 547 0.120 - 666 0.584 Yes 684 0.369 - 712 0.399 - 719 0.634 Yes 739 0.349 - 751 0.463 - 765 0.623 Yes 772 0.522 Yes 808 0.504 Yes 876 0.498 - 888 0.595 Yes 897 0.401 - 1090 0.047 -

Explain the output -- Processing locus 730: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGGGAAAGTGTGGAACTCATGAGACCACAGGTGGAGAGCCTGAGTGCAACTCACGACACCTTTAACCAACCAAAACACGC CACGTTATCTCTCACTGTTAAATTCTCTCCTGTAAAAGAACTGGCAAATCTCCCTCAACAAAGAACTTAGCCTTAAAGGA TTTGTATAACAAATATTATCGTAATCGCTTAAGGCTCAAGTCACACAAGTTCATTGATTCCACCTTTCTAATTACGAGGA TGGAAGTTTCCGGCGTCGTCCTCCGACAAATTCCATGTGTTTCTTCAGGTTCCGTTGCAGGTCTCCGATTAGTATCGGAA TTTTCCGGTAATACCAGAACGGTAGGGTTTCGAACCAGAAGATTTCGTGGTATTGTGTGTAATAATGAGTTTGCGGATAA AGGGCATGTGAATTACTATATCGAGCCAACGAGATGCGGCGAGGAGAAAGAGAAGGTTAAGGTTATGGAGAAGGAGAAGA AGGCGTTGAAGAAGAAGGCGAAAGTTCTTAAAAGTCTTTCTAAGAACTTGGATATGTTCTCTAGTATAGGGTTTGGTCTT GATCCTGAAGCTGGTTTGGTTGGTGAGATTCAGACCAAGACAATCTCGGAAGCTACAGAGATATTGGTTAAGCAGCTGGA GCAGTTGAAAGCAGAGGAGAAGATATTGAAGAAACAGAGGAAAGAAGAGAAGGCTAAAGCCAAAGCCATGAAGAAGATGA CTGAAATGGACTCTGAATCATCTTCTTCGTCTGAATCAAGTGATAGTGATTGTGATAAAGGCAAAGTTGTGGACATGAGC TCTTTAAGAAACAAGGCTAAACCGGTTCTTGAGCCGTTACAACCAGAAGCCACAGTAGCAACCCTTCCTAGGATCCAAGA GGATGCAATTTCTTGCAAGAACACCAGTGAAGCATTGCAGATTGCTCTACAAACATCCACAATTTTTCCTTCAATGGCCA ATCCTGGACAGACATTGAAGACGGTAGAAGCGGTTTCAGTGGTTGGATTACCATTGAATAGAGTAGAAGTGTGTATGGGA GGGAAGTGTAAGAGATCAGGAGGAGCTTTGTTGTTGGATGAGTTTCAAAGGGCGATGACGGGTTTCGAAGGATCTGCTGT GGCTTGCAAGTGCATGGGGAAGTGTCGTGATGGCCCAAACGTTAGAGTTGTGAAGGAGACTGATGCTGTGATGACTGATT CAGTTAGGACACCATCCAAGACCCTTTGTGTAGGAGTTGGATTGCAGGATGTGGAAACTATTGTGACAAGTTTCTTTGAT GAAGAATGTAGCAGAGAAGGACTCGGTTCTGTCTCATATTGAAAGTGTTTTGAAACGGCTAATGTTTTATATAGTGTGGT GCTTTCTCATTGTACATATTTACCTTCTCGATAAGTCATTGATATAAGCCATGTAATTGCG ..................i............................................................. ................................................................................ ...............................................................................i ..................................N............................................. ................................................................N............... .....N...........................N..............................N............... .....................................................i.......................... ................................................................................ ...................................................................N........N... .....i....................................................................i..... ................................................................................ ..N......................................................................i...... ..........................................................................N..... .....................................N................i......................... .............N...............N................................N.......i......... ................................................N.............................N. .....N.......................................................N.................. ..................................................N.......... Pos Score Pred ------------------------ 19 0.610 Yes 240 0.549 Yes 275 0.348 - 385 0.474 - 406 0.164 - 434 0.409 - 465 0.379 - 534 0.622 Yes 708 0.375 - 717 0.409 - 726 0.732 Yes 795 0.702 Yes 883 0.198 - 954 0.538 Yes 1035 0.378 - 1078 0.253 - 1095 0.651 Yes 1134 0.454 - 1150 0.307 - 1183 0.214 - 1191 0.654 Yes 1249 0.471 - 1279 0.159 - 1286 0.297 - 1342 0.117 - 1411 0.037 -

Explain the output -- Processing locus 731: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGAAACGAATAATAAAAACAACCATAAGCCTACCAAAAGGAAAACATTATCGTAATATAATCAATCAATAACACGTAT ACAATTATTAACGTATATTGACAAGCAAAATTAATGAGAGCACTCACTATAGCTATAGTCTCTCTATATAAACAACTTTC ATTCGTCTCTTCAGTCTTCACAAACACAACATATCCACAATACAAAACACAACTTTCATATATAACAAAAAAAGTTATAG AAATGGCCAAAGACGTGGAAGGACCTGAGGGATTTCAGACAAGAGACTACGAAGATCCGCCACCAACTCCGTTTTTCGAT GCGGACGAGCTTACCAAGTGGTCTTTATACAGAGCCGTCATTGCCGAGTTCGTAGCCACTCTCCTCTTCTTGTACATCAC CGTTTTAACTGTCATCGGTTACAAGATTCAGTCCGACACAAAAGCCGGTGGAGTTGACTGCGGCGGCGTCGGAATCCTTG GCATCGCGTGGGCTTTTGGTGGCATGATCTTCATCCTTGTCTACTGCACCGCCGGTATCTCAGGTGGTCACATAAACCCT GCGGTGACGTTTGGTTTGTTCTTAGCCCGGAAGGTATCGCTGATTAGGGCGGTGCTTTACATGGTGGCTCAGTGTTTGGG TGCTATTTGTGGAGTTGGTTTCGTCAAAGCCTTTCAAAGCTCTTACTATGATCGTTACGGTGGAGGAGCCAACTCTCTAG CAGACGGCTACAACACAGGCACCGGACTAGCCGCAGAGATCATTGGAACATTCGTTCTCGTCTACACAGTCTTCTCCGCT ACTGATCCCAAACGTAACGCTAGAGACTCCCACGTTCCGGTTTTGGCGCCACTTCCGATTGGGTTTGCGGTGTTTATGGT ACATTTGGCCACTATTCCGATCACCGGAACCGGCATCAACCCGGCTAGGAGTTTCGGAGCTGCCGTAATCTATAACAAGA GCAAGCCATGGGATGACCACTGGATATTCTGGGTGGGACCATTCATTGGAGCTGCGATAGCTGCATTTTATCACCAATTT GTCCTAAGGGCTTCAGGTTCCAAGTCACTTGGATCCTTCAGAAGTGCAGCCAACGTTTGAGTTTTTGCCACACAAAAAAA AACCTATCATAATAACAACAATTACAAAATGGTTGTGTTATTATGTATGTACAAAAATTGGCAACTTTGCTTGTAAAAC ................................................................................ .................................N.............................................. ................................................................................ ..i...........................................................................N. ................................................................................ ................................................................................ .......................N........................................................ ............................................................i................... ...............................................i................................ ................................................................................ ...........................................................................i.... ................................................................................ .......i....N................................................................... ................................................................................ ............................N.............N...N................................ Pos Score Pred ------------------------ 114 0.271 - 243 0.800 Yes 319 0.083 - 504 0.162 - 621 0.512 Yes 688 0.570 Yes 876 0.747 Yes 968 0.727 Yes 973 0.235 - 1149 0.105 - 1163 0.130 - 1167 0.037 -

Explain the output -- Processing locus 732: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTCTGGTCTTTTTCTTGTTTATATAATTTGAAGAGGATACTGTTATTCTTTTTCTTGTGATTGGCATATATATATATAT ATATACATATGTGCCTAATAGCTTATGTTAAAAATATTTTGCAGCTTGACAATGCCTCGCTATGATGATCGCTATGGAAA CACTCGCCTCTATGTTGGTCGCTTATCATCTAGAACTCGTACCAGAGACCTTGAGCGTCTCTTTAGCAGATACGGAAGAG TACGAGATGTGGATATGAAGCGTGATTATGCCTTTGTTGAATTTAGTGATCCTCGTGATGCTGATGACGCAAGATATTAC TTGGATGGACGGGATTTCGATGGAAGTCGCATCACTGTGGAGGCATCAAGAGGGGCTCCTCGTGGTTCGCGGGACAATGG TAGCAGAGGCCCACCTCCTGGTTCTGGTCGCTGTTTTAATTGTGGTGTCGATGGCCACTGGGCCCGAGACTGCACAGCAG GAGACTGGAAGAATAAATGTTACCGCTGTGGTGAAAGAGGACACATTGAGAGAAACTGCAAAAACAGTCCTAGTCCAAAG AAGGCCAGGCAGGGTGGAAGCTATTCCAGGTCACCAGTCAAATCCCGCTCCCCTCGTCGCCGAAGGAGCCCAAGCCGTAG CCGTAGTTACAGTCGAGGTCGCAGCTACAGTCGATCGCGATCCCCAGTGAGAAGAGAGAAAAGCGTGGAGGACAGATCAC GCAGTCCTAAGGCAATGGAGCGATCTGTATCTCCCAAAGGTAGGGACCAAAGCCTGAGTCCAGACCGAAAAGTGATAGAT GCAAGCCCAAAGCGTGGATCAGACTATGATGGTAGCCCAAAAGAGAATGGTAATGGCAGGAACTCTGCGAGTCCCATTGT TGGAGGTGGTGAAAGTCCTGTTGGACTTAATGGTCAAGACAGGAGCCCGATTGATGATGAGGCTGAGCTTAGCCGTCCTT CCCCTAAAGGCAGTGAGTCACCTTGATGGTTCACAGGTTAAAAGCTCTATGTAGCATTCTGTACTGAAGGATGTTGTTTC CTTTCCCTTTCTTTCTGCGTGATACTTTTCTTTTCGTTGCGTGAC ................................................................................ ........N...............N..........................i.........i..i........N...... ...........N.................................................................... ......i.......i............i.............................N.....N................ ....N..............N........................................................i... ..................................................i............................. ................i............................................................... ................................................................................ ................................................................................ ..............i...............................................................i. .........................i..N.................N.....i........................... .............................N.......................N..N....................... .........................N......................N.....................N......... ............................................. Pos Score Pred ------------------------ 89 0.181 - 105 0.271 - 132 0.569 Yes 142 0.678 Yes 145 0.799 Yes 154 0.459 - 172 0.249 - 247 0.629 Yes 255 0.729 Yes 268 0.645 Yes 298 0.398 - 304 0.156 - 325 0.296 - 340 0.277 - 397 0.687 Yes 451 0.752 Yes 497 0.527 Yes 735 0.541 Yes 799 0.610 Yes 826 0.571 Yes 829 0.480 - 847 0.499 - 853 0.551 Yes 910 0.266 - 934 0.257 - 937 0.271 - 986 0.085 - 1009 0.078 - 1031 0.080 -

Explain the output -- Processing locus 733: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTCATCGAGCGAGCTCAACATTTTTCGGACAACAAAGTGTAAGAGACTGATTAAACAAAATCGAGACTTTATAGTACTC AGGTTTGTCGGAATCGACTGAGTATCGAAATTATTGTGGTGTTTTGTGAACAGAAACCAAGGTATGCATGAAATCGTTTT GTATATAAACCCTAATATCGATTTCAGAATTTTCATTGATCTCGTTGTTTTAGCAGGATTAAAGATGGTTCAATCTGTGT TAATTTCGCGTAACAAGGGTTTGGCGGAGCAAGATCTAACTAAGCTTGATGTAACAAAGTTACATCCTCTGTTTCCTGAA GTCATTTCTCGCCAAGCAACCATAAATATCGGAACCATTGGTCATGTCGCTCACGGTAAATCGACTATAGTGAAAGCTAT CTCTGGTGTTCAGACTGTGCGTTTTAAGAATGAATTAGAGCGTAACATTACCATTAAGCTTGGTTATGCAAATGCAAAGA TTTACAAGTGTGAGGATGACAAGTGCCCTAGACCAATGTGCTACAAGTCATATGGGAGTGGAAAAGAAGACAACCCAACT TGTGATGTCCCCGGATTTGAGAATTGCAAGATGACACTACGGAGACATGTCTCATGTGTCGATTGTCCGGGACATGATAT TCTTATGGCGACAATGCTCAATGGAGCAGCAATCGTGGATGGTGCTTTACTTCTAATTGCTGCTAACGAGAGTTGTCCAC AACCACAAACCGCTGAACATCTTGCTTCTGTTGATATGATGCGCCTTAAGCATATCATAATCCTTCAGAACAAGATTGAT CTCATTAATGAGAAAGCCGCCACTGAACAGCACGAGGCTATTCAGAAATTTATAACGAACACGAATGCTGAGGATGCACC TATAGTTCCTGTCTCAGCACAACTGAAATACAACATTGATGTTGTGAGTGAATACATTGTCAAGAAGATTCCAATCCCTG TGAGAGACTTTGTGTCACCACCAAAGATGATTGTGATTCGTTCTTTCGATGTCAATAAACCTGGCTCGGCGGGTCATGAA ATGAAAGGTGGTGTTGCTGGTGGAAGTATCCTCCAGTGAACCAACTGATTGAAATTAGACCTGGTATCACTGGCAAAGAT GAGCATGGAAACTCAAAATGCACTCCAATTTACTCGCGTATAACTTCACTATACGCGG ................................................................................ ...............................................................N...N............ ................................................................i............... ................................................N............................... ...........................................N.................................... .............................N...................................N.....N........ ...............i...................N...............N............................ ....N.........................i...............N......N...................N...... ....i........i......N.................N......................................... ...................................i..i......................................... .......N........................................................N........N...... ......................................N......................................... ..........................i.....................N..........................N.... N.............................................................................N. ....N............N........................................ Pos Score Pred ------------------------ 144 0.425 - 148 0.057 - 225 0.566 Yes 289 0.141 - 364 0.194 - 430 0.493 - 466 0.357 - 472 0.376 - 496 0.634 Yes 516 0.483 - 532 0.474 - 565 0.140 - 591 0.547 Yes 607 0.265 - 614 0.178 - 634 0.124 - 645 0.840 Yes 654 0.782 Yes 661 0.321 - 679 0.174 - 756 0.744 Yes 759 0.657 Yes 808 0.157 - 865 0.184 - 874 0.061 - 919 0.165 - 987 0.694 Yes 1009 0.454 - 1036 0.147 - 1041 0.448 - 1119 0.132 - 1125 0.065 - 1138 0.145 -

Explain the output -- Processing locus 734: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACGACGAGAGAGCAATCGCCATTGTTGCTTCCAGAGAAAGCTGAAAATAATAATAATCCCAATTTAAAATCGAAATCGA TTTTCTCATACCGTTGGTTAATCTCGTGGAGTTGGGTTATTACACGTGTCAGATGATGTAACCATGATCTCAGAGAAAAA GTCACATGCCACGTCAGTTAACTTTAGGGCAAAATGGGAAAAGAATCTAAAGAGCATATGTTCGTGCACGTTCTTGTTTA GGGTAAGCTCAAAGCTCAAGCCGCTTCTATCTCTCTACATTTCTCTTTCTGCAATGGCGAGATTAGTGCGTGTGGCTAGA TCCTCCTCCCTCTTTGGCTTTGGTAACCGTTTCTACTCTACTTCAGCCGAAGCTAGCCACGCGTCGTCGCCTTCGCCGTT TCTTCACGGCGGCGGAGCTAGCAGGGTTGCTCCGAAAGATAGAAATGTTCAGTGGGTGTTTTTGGGATGTCCTGGTGTTG GAAAAGGAACTTACGCTAGTAGACTATCAACCCTTCTCGGCGTTCCTCACATCGCCACCGGCGATCTCGTCCGTGAAGAG CTTGCATCTTCTGGACCTCTCTCTCAAAAGCTATCGGAGATTGTAAATCAGGGAAAATTGGTTTCTGATGAGATCATTGT AGACTTATTGTCCAAAAGACTTGAGGCTGGTGAAGCTAGAGGTGAATCAGGGTTTATCCTTGATGGCTTTCCTCGTACCA TGAGACAAGCTGAAATACTGGGAGATGTAACTGACATCGATTTGGTGGTGAATTTGAAGCTTCCTGAGGAAGTTTTGGTT GACAAATGCCTTGGAAGGAGAACATGTAGTCAATGTGGCAAGGGTTTTAATGTAGCTCACATCAACTTAAAGGGTGAGAA TGGAAGACCTGGAATTAGTATGGATCCACTTCTCCCTCCACATCAATGTATGTCAAAGCTTGTCACTCGAGCTGATGATA CTGAAGAGGTGGTGAAAGCAAGGCTTCGTATATACAATGAAACGAGCCAGCCTCTTGAAGAATACTACCGTACCAAGGGA AAGCTTATGGAGTTTGACTTACCTGGAGGCATCCCAGAGTCATGGCCAAGGCTATTGGAAGCTTTAAGGCTTGACGATTA CGAGGAGAAACAGTCTGTCGCAGCATAAGTTCACTATCAAATCCCGGTATTTAAAATTCTTTGCATGTGGAATACATATG CGTAATCAATTTTGTGTTGGAAATAAAAGTAAAAAGAAAACCTTAGA ................................................................................ ....................................................N..N.......N................ .....N...........................i.......................i...................... .....................................................i.......................... ................................................................................ ............................................i.....................N............. ................................................................................ ...................................................................N............ ..............................................................N................N ........................N....................................................... .....i.................N........N................N.............................N ...................N.........................N...N........................N..... ....................................N........................................... ......i..................................N...................................... ................................................................N............N.. ............................................... Pos Score Pred ------------------------ 133 0.110 - 136 0.085 - 144 0.355 - 166 0.332 - 194 0.690 Yes 218 0.765 Yes 294 0.798 Yes 445 0.573 Yes 467 0.240 - 628 0.294 - 703 0.154 - 720 0.363 - 745 0.381 - 806 0.655 Yes 824 0.465 - 833 0.164 - 850 0.321 - 880 0.490 - 900 0.461 - 926 0.348 - 930 0.417 - 955 0.177 - 997 0.178 - 1047 0.608 Yes 1082 0.283 - 1185 0.066 - 1198 0.065 -

Explain the output -- Processing locus 735: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCTAAGTATGTAACTATGTCTGGTATGTGAGCTGGGATCATATGATACCTGACTATCGACTCAAAGAGAATTATGTTAAG TCAATCAAGGCTTAGTTCTGAGGCTACAGTGTGTGTGGTCGATGTGCTGTTATGGTGGAATTGGATACTAAAATATGTCT AAGAGTGGGACTGCAATATGACCTTCTTAGTTTATTCGATGTTTTGGTTGCAGAAGAGGAGGTCATGGACGTTTCTGCTA GAAAGTCACAAAAAGCTGGGCGCGAAAAGTTGAGGAGGGAAAAACTGAATGAGCATTTTGTTGAACTGGGAAATGTACTC GATCCAGAGAGACCCAAGAATGACAAAGCCACGATTCTGACTGATACTGTTCAGTTGTTGAAAGAGCTCACATCTGAAGT CAACAAACTGAAATCTGAGTACACCGCATTGACAGATGAGTCCCGCGAGTTGACACAGGAGAAAAACGACCTGAGAGAAG AAAAGACATCGCTGAAATCAGATATAGAGAATCTCAATCTTCAATACCAGCAGAGATTAAGGTCAATGTCTCCATGGGGA GCTGCGATGGATCACACAGTCATGATGGCTCCACCACCCTCCTTTCCATACCCTATGCCTATTGCTATGCCTCCCGGGTC AATCCCAATGCATCCATCAATGCCATCTTACACATACTTTGGGAACCAGAACCCTAGCATGATCCCAGCTCCATGTCCTA CATACATGCCCTACATGCCTCCTAATACAGTCGTTGAGCAACAATCCGTGCACATTCCACAGAACCCCGGTAACCGTTCT CGGGAACCTAGAGCAAAGGTTTCAAGAGAGAGCAGATCTGAGAAAGCAGAGGACTCCAACGAAGTTGCAACACAACTCGA ATTAAAAACCCCTGGATCTACTTCTGATAAGGATACATTGCAAAGGCCAGAGAAGACAAAGAGATGTAAGAGAAACAACA ACAACAACTCAATAGAAGAAAGCTCTCATTCTAGCAAGTGTTCATCTTCTCCGAGCGTACGAGACCACAGTTCTTCCAGT AGCGTAGCTGGTGGCCAAAAACCTGATGATGCAAAATGATTCGAAAGAATCTGATGTTGATCATCTCAAGTATCCAAGTA TCGTTTCGATGAGTACTGTATATAGTGCGAGTACAAAATGCACTTAGCTGTTTAAAGC .......N.......i........N................N..............................N....... .........................................N.........i......................i..... .................N....................N.........................i............... ................................................N.......................N....... ...................N............................................................ ...................................N............................................ .................................................................i.......i...... ......i..............i..N.............................N...........N............. .......i...........i......................................i.............N....... .....i........N................................................................. ................................................................................ ...............................................................N................ ................................................................................ .........................i..i......N.................N.......................... ........N............................N.................... Pos Score Pred ------------------------ 8 0.103 - 16 0.659 Yes 25 0.314 - 42 0.218 - 73 0.288 - 122 0.206 - 132 0.779 Yes 155 0.505 Yes 178 0.368 - 199 0.305 - 225 0.739 Yes 289 0.091 - 313 0.150 - 340 0.274 - 436 0.493 - 546 0.706 Yes 554 0.721 Yes 567 0.572 Yes 582 0.511 Yes 585 0.482 - 615 0.241 - 627 0.238 - 648 0.583 Yes 660 0.736 Yes 699 0.688 Yes 713 0.491 - 726 0.648 Yes 735 0.460 - 944 0.370 - 1066 0.568 Yes 1069 0.677 Yes 1076 0.451 - 1094 0.092 - 1129 0.092 - 1158 0.066 -

Explain the output -- Processing locus 736: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTGTTCTTATTGGTTTGTTGTTTATGTAGTTAGAGTTGTTGATTATAGAAAAGTTTTAAGTCTTTTGATTGGATTTAGG ATTTATTGATAATCTACTTAAAATATGTTTCTTGATTTGATGAATTCGCAGTTAAAACATCGAAAAATGCAGGACGATGT CATTGATGTATCCCTCAATACAAAGAATGTCTTCTTGTCAGCTCTTCATTTCGCCATGTCCATTGATACACTAAAGAACT CTCCTGAGCTTGTTGATGAGCTGAAAACATCAGCTCAAGAACAAGTGGAGTTTATGTTATCTGAAGACGAAGAGGTACGG TTACTGATTTCCGAAGAGGAAGTGAAATCCGTTGTGAGATTAGGTATCTCAAATGTTATATCGATGTTATTGGATAGATT ATCATCATTGCTTGTGATTCTTCCTGAATGTACTGAGTTTAGTGTTCTTAAGACATTATATGATATTGAGTGGTTGTGTA AAGTATTGCCAAGGATGGAGTTGATGAAAGATCTTGTCGTTAAGTGGACCGATGCATCGAGTGAGATTCTTGTGATTGCT CAGAAGTGTGAGTTGGATTCTAGGATATTGGGTGTGAAGGTTAAGCTAGTTGAGGTTACAGGGAAGATTTTAGAAGCGGT AGGTTATGGAATTGTGATTGTTCCTTCTAAAAGCAGGACTTGTCTTTTGAAGATATGGTTGCCTTTCATAAGGAGATTAA AGACTTTGGTTGATGCAGAGGGATGTGAGTATGAGTATAGAATGGATGAAGATCTTTGTGAATTTATAGAAGGATCTATG GTGTCTTTGGTTTTGACATTGCCTTCAAATGACCAAGCAGAAGTGTTTGGTGAATGGATGAGAGGTATTGGGTTGGAAGG TGTGAAGTTTCCGGATTTAAGTGAAGCGTTTGAGGTTTGGTGTTATAGAAGTAAGTCTGCAAAGAGAAGATTGGTGGAAA GATGTGATAGACTTGCAATTGTAAATCTTGACCATTGAATGGTGTATATCAGTTTCGAGATTCGGTAAACTTTAAATGAA GATGTAGCTAAGCAATTTGAAGTTCCAGGCTTTTCTTCTTCCTACAAGCTGGTCTGC ........................N....................................................... ........................i..............N..........................i.........N... .....N....................i............................N........................ ...............N.....................................N.......................... ....................................................N..........N................ ...........................N...............................N.................... ..............i........N...........................N............................ ................................................................................ .....N................................................N......................... ............N.........N.......N..........N...N...............................N.. ............................N........................N...i...................... ................................................................................ Pos Score Pred ------------------------ 25 0.093 - 105 0.644 Yes 120 0.231 - 147 0.691 Yes 157 0.147 - 166 0.242 - 187 0.626 Yes 216 0.335 - 256 0.105 - 294 0.416 - 373 0.118 - 384 0.108 - 428 0.268 - 460 0.091 - 495 0.595 Yes 504 0.381 - 532 0.078 - 646 0.176 - 695 0.358 - 733 0.264 - 743 0.198 - 751 0.207 - 762 0.427 - 766 0.149 - 798 0.415 - 829 0.394 - 854 0.312 - 858 0.540 Yes 962 0.258 - 999 0.367 - 1036 0.057 - 1042 0.085 -

Explain the output -- Processing locus 737: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCAAAGCCTGATCCTGTTTTTCATAAATTACATCTAAGTACAGCTCTATTTTTCCAACTCATTAGATATAAACCAATCA TTCTGTAATTGTTTCAACTAATTTCTGTTTCACATGTATAAAAGCTCTTCAACCTCCAAGTTCTCAACCCACCAAAAATT TCAATTTCTTTTCTACTTTTCTCCAAATAAAAAACTCACATCCACATTTTTCTTAAAGAAAATGACTTCTCATGGTGAAG AGATCGAAGACGAACAAATATCAAGAATTGAGAAAGGCAATTGCAAAGATAGCCAAGGAGGAATGGAGACGGCCATATGT AGTTCTCCTAGCATTGTTTGCCTTACACAAAAGTTGATAGCAGAGATGATTGGAACCTATTTCATAATATTCTCTGGATG TGGAGTAGTGGTAGTGAATGTCTTGTACGGAGGAACAATAACATTTCCAGGGATCTGTGTGACTTGGGGTCTCATTGTTA TGGTCATGATTTACTCTACTGGTCACATTTCCGGTGCACATTTTAACCCTGCCGTCACTGTCACGTTCGCTGTTTTCCGG CGGTTTCCTTGGTATCAGGTACCATTGTATATAGGTGCACAACTTACGGGATCATTACTAGCGAGTTTAACATTGAGACT AATGTTCAATGTGACACCCAAAGCTTTCTTTGGAACAACTCCAACTGATTCATCAGGACAAGCACTAGTAGCAGAGATCA TTATCTCATTCCTCCTCATGTTCGTTATCTCCGGCGTTGCCACTGATAGTCGTGCGACTGGAGAATTAGCTGGAATCGCG GTGGGGATGACTATAATATTGAACGTCTTTGTAGCCGGACCTATTTCGGGAGCATCAATGAATCCAGCGAGAAGTCTTGG ACCAGCAATAGTGATGGGAAGATATAAAGGAATTTGGGTTTACATTGTTGGTCCCTTTGTCGGAATTTTTGCCGGAGGTT TTGTTTACAACTTCATGAGATTTACGGATAAGCCACTACGAGAACTCACAAAAAGTGCTTCTTTCCTCCGTAGTGTTGCC CAAAAAGACAATGCTTCTAAATCAGATGGCTAATTTGTTTTTTCTTTGTTTGATATCAGTTTTACTGTGAATATTTGTAT CCATTCCTTATTCGTTAATTAACTTAATTTTTAGTTGAATAAAATCTGAAAA ................................................................................ .................................N.............................................. .............................................................i.........i........ ..............................................................i.............N... .............................................i...............................N.. .................N.............................................................N .....N.......................................................................... ................................................................................ .................i.............................................................. ......N..................................................N...................... .............i.................................................................. ..............N................................................................. ..........N..............N...................................................... .................................................... Pos Score Pred ------------------------ 114 0.343 - 222 0.684 Yes 232 0.590 Yes 303 0.740 Yes 317 0.364 - 366 0.505 Yes 398 0.429 - 418 0.264 - 480 0.385 - 486 0.375 - 642 0.285 - 649 0.254 - 738 0.595 Yes 807 0.290 - 858 0.391 - 894 0.631 Yes 975 0.105 - 1051 0.119 - 1066 0.125 -

Explain the output -- Processing locus 738: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACAACTCTCAACCAAAAAACTACGTCGTTTAATCTTTATCTGACCGTTACACCAGAATTACCATTTTACCCTTCTTCC ATTGTTTTATCCACACTTTAAAGATTGAAACTTTTTTTTTCTACCTCACCGACGATGATGATAGCAAGGTGTTCTCTTTT TCCGCCGCCGTGTTTCTCCGCCGTGAGGATTAGATGTTTCGCCGGAGAAACTTCAGATACTGGGATTCTATTCCGGGAGA AGCTTATTTACCTTCAAGACCTTAACGTAGATCCTCACAAAGCTCTCCGAGTAAACCCATCTCTCCGTTCTGCTCCAATC TCCTCCGTCGTCTCCGTCGAGACTCTACTCTCTTCCACCGGACTCTCAAGACCCGCCGTTGGTCGTATCCTCGACATGTT TCCAGATTTGTTAACTTCTGATCCTGAATCTGAAATCTTACCAGTTCTTCGCTTCTTATCCAATGAGATTTCAATTTCAG AGCAAGACATACCTAAATCGATCTCTCGTTGTCCTCGATTGCTAATTTCATCTGTAGATTATCAGCTTCGTCCTGCATTA ACGTTCCTCAAAACCCTAGGATTCGTGGGTCGCGACACGATTACGTCACGGAACACGGTGTTGCTTGTATCGAATGTGGA ACGAACCCTAATCCCCAAAATTGAGTATCTTGAAGAAGGGTTAGGGTTTACAAGAGAAGAAGTAGCGAAGATGGTTGTGA GATCTCCGGCGTTGTTGACTTATAGTGTTGATAACAATTTAGTTCCTAAAGTTGAGTTTTTTATTGAGGAGATGCGTGGA GATGTTAAGGAGCTGAAACGTTTCCCTCAGTATTTTTCGTTTAGCTTGGAGAGGAAGATAAAGCCAAGGCATAGATTGCT TAAGGAACATGGGATTTTGATGCCTTTGTCTGAGATGTTGAAGGTTAGTGATGGTCAGTTTAACCATTGGCTTTTAGAAC TTCGTCTTAGGTCTGCTGAAAGAAGATGATGATAAACAACAACGTTCTTTTTGATGTTCTTTTTACTTGAGATTTGGGAC ATGTCAAGACACTTCACATGAGTTTTGTATATAGTTTCTGTTTCAATG ................................................................................ ......................................................N..i...................... .................................N.............................................. ................................................................................ ...........................................................................N.... ..............................................................N................. ................................................................................ .........................................................................N...... ......................................................................i......... .......................................................................N........ ........N..........N..............N...............N............................. .........................i..N........................N.......................... N................N...........................N.. Pos Score Pred ------------------------ 135 0.416 - 138 0.677 Yes 194 0.434 - 396 0.485 - 463 0.187 - 634 0.209 - 711 0.791 Yes 792 0.366 - 802 0.165 - 889 0.172 - 900 0.438 - 915 0.293 - 931 0.272 - 986 0.514 Yes 989 0.307 - 1014 0.103 - 1041 0.064 - 1058 0.073 - 1086 0.040 -

Explain the output -- Processing locus 739: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATTTGCAACTCTCTGTCTTCTCTACTACGTTACTAATATATTCTACTGGGCTTTAAGGCCTCAAATTACAATATGTTT GGTTCCAAAATTTGTATTTAATGGGCTTCTCTTTCTTTAGGTTTTTATAATAAACCGGGTCAAACAGTACCCGACTCGTC TAATTTTCCCGCTAAAGAAAAAGTGAAAACCGCCCAAATAATTTTCGATGGTGGAATGATAAAAGACCTAAGAGGGAGAA AGAGAGATCGAAGAGTGAAATCTGAAATGGAATCAGCTCCGACGGGATGCTTCAAGTGTGGTCGTCCCGGTCATTGGTCT CGTGATTGTCCATCATCGGCTCCGGTCGCCGGCAATAATTCCGTTTCTTCTTCTTCCGCTCCATCTCAAATCCCTAATAA TGAATTTCAGAGATCCTCCAGTAAAAGTGGAACCTCAATAGCTCCTGCTCCTAAAGTGACGAAGACGAGAGTTCAAAGGC CCAAGCTAACTCCTGAATTGTTACTCTCTGAGGATGGTCTCGGCTATGTTCTTCGCTATTTCCCTAAATCCTTCAAGTAT CGTGGCCGAGGCAAAGAGGTGAGCGATTTGGGGAATTTGATACGGTTGTACAGTGAGTGGCACACTCATTTGCTACCTTA CTATTCCTTTGATCACTTTGTGCATAAAGTTCAACAAGTCGCTTCTACTAAGCGTGTTAAGAATTGTATAAATGAACTGC GAGAAAGGGTTGCCAGTGGAGTTGATCCAAACAAGTTATACGAAAAGCAGGAAGAAAATACTGTTCCTTCTGATGATCAA GATATGGATCAGCCAAGTCATGACGAAGAGAACATACCATCAAAGAGCGTTGATGCTGATACAAATGCTGATGCTTTTGA AGACAGTATGTTGAATGAGATATTTGATAATGCTTCAAAGTTACCGAGCGATGAACAAAACATGGATAAAAGCAGTGAAC TGACAGAAGAGCAAAGAGCACGTATGGAAGCTAATAGATTGAAGGCAATGGAGAAAGCTCAGAACATTTCCGAGGAACAG AGAGTACGTATGGAAGCTAATAGGCTAAAAGCTCTCGAAAGAGCCAAAGCAAGGCTCCAACCAAATCAAGATTAGACTAG AGATGTTCAATTTGAATTTTGGATTGAAAAATTTGTGCTTTTGAATGTTTCAATTAGTTATCTAATAGTAACAAATGTGT GTCCGAAACTAGAA ..........................................................................i..... ....................N........................................................... ...............................................i.......N........................ ..........................i...................N................................. ...............................................................................i ................................................................................ .................................i...........N.................................. ................................................................................ .......................................................................N........ ........................................................................N....... ...i...............N................................N...........N.....N......... .......i......N..............N....................N..........i.................. .......................i.......................i................................ .........i...................................................................... ..N.........................................N.............................N..... .............. Pos Score Pred ------------------------ 75 0.659 Yes 101 0.417 - 208 0.685 Yes 216 0.189 - 267 0.573 Yes 287 0.156 - 400 0.511 Yes 514 0.624 Yes 526 0.431 - 712 0.347 - 793 0.090 - 804 0.728 Yes 820 0.142 - 853 0.355 - 865 0.287 - 871 0.100 - 888 0.722 Yes 895 0.109 - 910 0.151 - 931 0.234 - 942 0.718 Yes 984 0.653 Yes 1008 0.734 Yes 1050 0.591 Yes 1123 0.136 - 1165 0.050 - 1195 0.089 -

Explain the output -- Processing locus 740: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGTTTAAACCACGATACGATTACACGTACCGTCGTATATGCATGTAGTTGTAATTACATTTTTTACTTAAAAGTATATGA CGACAGATAAAAAAACTTTAAATTGTACATACATGCACCAATCCAATGTATAAAAGGCAAAAGAATAGCGTTAAAATGCA TTCACGAACTAACCAACGAAGAAAGATGGCGACTAGTTCTTCGAGTTATGGGATTGAACAACTTCAAAAAGGATGTTACT ATGAGTGGATGAGTGTGCAGGCTAAACACATAGTTGATCTCAAAGAAGCACTCATGAGTCATCGATCCAAAGAAGATCAC AAGCTTGAAGAACTCGTTGGTAAAATCGTCAATGACTTCCAAAAATACACCGAAAAACGATCTGAGCTCTCTCGCCGAAG CTGCTCAAGCTACTTTGCACCGTCGTGGAACTCTCCTCTAGAGAACGGTTTACTATGGATGGGAGGATGTCGTCCATCGT CTTTCATTAGAGTTATTTACTCTCTTTGTGGATCCCAAGCCGAAACGCAGCTTTCTCAGTATCTTCTAAAGATCGATGAA AATGTCGAAGTAAACCATGGTGGTTCCATGAGTGATCTAAACGCGTCGCAGCTTGCAAAAATCAATGATTTGCATATAAA GGTTATAGAGAAAGAGGACAAGATAACAAAGAAATCGGCGAATTTGCAAGAGAATGTTGCGGATATGCCTATAGCCATCG CGGCTTATGCCACGGACTTGATGAATGGTGACGTGGTGGTGGAAGATGCTTTAGATAAGTATGAGGAGGGCATGGCGGTT TTAATGGTGGAAGCTGATAAACTGAGGTTTGAGACGCTTAGGAAGATCGTGGATGTTGTTACGCCGGTTCAAGCGGCGGA GTTTTTGCTCGCAGGGAAAAGATTACACATATCATTGCACGAGTGGGGAAGAGTTAGAGAAGAGCAACGTTTTGGGTGTG TACGTACGGATGCAGCGGCTGCCACGGGAGGAGCGGGAACCGAGAAGTCAAAGAGGTCAAGCTTGTTGATGTGAGTTTAA AGTTGAATATATATAACTTGTGTTATATTTGTCTATGTAGAGATAAAATCGTGTTTAAGTGTCTTTATGTGTAATATATG TATGAGAGAGTGC .....................................N...N..................................N... ................................N............N.............................N.... .........................i.....................N........................N....... N.......i............................................i.......................... ...............................N................................................ ......................................................N...i.......N............. ...........................................................................N.... .....................................................N..........i............... ......N.............N...N....................N..............N..........i........ ...N................................................N........................... ................................................................................ .........N..........................................................N........... ..................................N...............................N..........N.. Pos Score Pred ------------------------ 38 0.474 - 42 0.235 - 77 0.312 - 113 0.259 - 126 0.469 - 156 0.301 - 186 0.768 Yes 208 0.258 - 233 0.336 - 241 0.438 - 249 0.546 Yes 294 0.596 Yes 352 0.497 - 455 0.422 - 459 0.536 Yes 467 0.267 - 556 0.221 - 562 0.693 Yes 577 0.514 Yes 588 0.455 - 625 0.150 - 694 0.114 - 705 0.630 Yes 727 0.060 - 741 0.396 - 745 0.147 - 766 0.135 - 781 0.087 - 792 0.760 Yes 804 0.368 - 853 0.170 - 970 0.178 - 1029 0.214 - 1075 0.082 - 1107 0.054 - 1118 0.041 - 1122 0.040 -

Explain the output -- Processing locus 741: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTGTCAACTAGAAACTTCATTAATGGCTCAAGTAAGAAACCATAAACAGAGTATCATATCAAAGGTAAGTAAACCAGAG AGAAGATCATCAACAAAATAAAAATTTACACCTGTAAAACCTCAAATTCTCGAATTGAAACAAATTAAAGTTATGTTTGG GCCCTTTTGTTCCAGGCCCATGAAAATTGTCCGTGCCGACAAAGTCAGTTTTTTTTGGTTCTTCGAGAAAGAAAAGAAAA ACAAAATGAAGAAAAGAACAAATCCGGAATCGTCCACCTCTGAAGCAAAGAAACGCCGGCGAGAGGAAGATGACGATAAA GTTGGTTGTAGTCACTCTGCAATTGAAGATGGAGCAGTATCTGAGGATCAATGTCTTCCTCTTGAGGATGATCTCACCTT TAGCGATACTTCTGTTGCCCTTCGAATGATGCGAGCTCAGTTTCCTCGTATTGACCAGGCTTCGATCCCACCCTTCATAT TACAGTCGCAATTGTACAGTAGTGTCAATGATAGAACCCAAGTCGATAGAGAATTAGAGTGTTTACGAAGGGAGAAAGTT GTTCGTGTTTTCAAACTCAACACTGGACAAGATGATCATGCCATCATCTTTTTGGATGACTATCTTAACCAGGTAGATCG AATAGTGAAGAGGATGGAAGAGAAGAAACAGAGTGATCTTGAAATTTTCAAGTTGTTCAAAATGCATGTCCTTGATTCAA AACTTGAACCTAGTATCGGGCATCATGAGCTACTTTCGCTGTTGTCATTGGGAGGAAAGGTGAAAGATGCACACATCACT CTCTTAATAAACGCAGGCCTTCTCACACGCCAACTTATCGATCCAGACATGTACTGGTTTGCAATTCCGAGTACCGGTAA ATTGTGGAAGGGTCTATCGCAGGGAAGGAACGAACTCCTCTCATTGATAAAGCGCAAGCGACATAAAGAGATGTTCTTGG CTGAGCTTGAAAAGAAACGGCTCCGGTTCTCTCCTCTGGATGTGAGATTTCACGTACGTGATCTCATTGGCTCCGGTCAC CTGAAAACTGTTCAGACAACTTCTGGACTGATCGTTCGGATTTTAAAGGACTAAAATGCAGGTGCAGGTTTCTCATCAAA CGGGTAATTTCATATTCAAGAGACCTTTTTCACAAAGTCACATGCTTTTCTCATTAGTTCAGCTTGCTACTAT .......................i........................................................ ........................................................................i....... ...................N............................................................ .....i...............................................................N.......... ............................N.....................N................N............ .........................i..i................................................... ...........................i.................................................... ...............................N.....N.................N........................ .............i...............................................N...N.............. ........................N.........................................N............. ................................................N............................... ......................................................................N......... .......................................N........................................ .......................................................N........................ .........................................N............................... Pos Score Pred ------------------------ 24 0.619 Yes 153 0.502 Yes 180 0.228 - 246 0.824 Yes 310 0.204 - 349 0.167 - 371 0.420 - 388 0.205 - 426 0.501 Yes 429 0.538 Yes 508 0.550 Yes 592 0.094 - 598 0.248 - 616 0.143 - 654 0.522 Yes 702 0.482 - 706 0.209 - 745 0.307 - 787 0.423 - 849 0.458 - 951 0.287 - 1000 0.288 - 1096 0.333 - 1162 0.046 -

Explain the output -- Processing locus 742: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCTTTCTCTATTGGAAATATTCTTTGTTCAATGTGTCTCCTCTATTAAAGTATATTAACTTCTTTACCTATGATGCAAG ACTAACATTAGAATAACTTGAATGCTTTCATCTTCCATTTGCAATCCAATTATTGTGTATAAAAGAAAGTTTCGAGAAGG ATTAAAAAAAAAAATCATTCCAATAGATAATTTAGGTAAGTATATGGTGAATCAGTCCAAGAACAAGTTTAGCATGAAAA CCAAGTATGGAGCAGCAGTTGCACCTGTATTCCTCCTTGCTGCTGCATATGTCGCTTGGAACTACATAAATCGTGGTTTA TGGAGAAAGAAAGACGATGAAAATGGTCGAGATACAAGAAAAAGCATCGGTAAAAACATTTCAGAACAAAGAAGAGATGG TAATGTTAAAAACATGGGTAAGGAGGCTTCTTCAGGAAGAGATGAAAGAACCCTCTCAAAATCTGTTTCAATGGGAGCTA TTCGAGGAGGAAAATTAGCACTACAGAGATTGCTTGATTTGCACTCATACCGCGGTGACACTTCTTCCCTTGCGAATGCT GAACTCGAATTTGAAGCATTGCTTTCCAAGGAAAAGCCGGACTTCGGGTTGCTTCAGAGAGACATTGTAAAAATGGAAAT GAGTGGAAAAGAAGCAAAAGGAGCTGAGCTATTGAAAAAGGCATTGGAGAAAGCAAGAAAAGAAGGGAAAGGTCATGAGG CTTATGAGATTGAGATGTTGCTTGTGGAGATGTTGATCTACTTGGGAAACATGGAAGAGGCTTCAAAATGTAAGTGTTTA GAGGATGAAGTCATAACAGATGCAAGACGTCCTCTATATCAGACTATAATCCATTACCTTCAAGGTCATCCTATGAAGCA AGTCGAGGAGACATTCAACAGGTTCAGAGAGATTCAAATTGGTCTACAATGGCCTGGAAGCTCAGAAGAATGTGAAATTG ACCAAGTCACATTTGATGAGTTCAAGAAAGTTATGGAATCTCTCAAGCATGAAATCGAAGACGGCAACAAAAGAAAGTTG AAGTTAGTCAAAGATAAAACTATGAGAGATAGTCTTCATTAATTTCAAGCAATATTAGTGTGTTATTATTCAAGAATGAT CATAAGATTTGGTACATAAATAATGATACTCTTGACAAAATACATCAAACTAGACCCAAAA ...............................N......................................N..N...... .....................N.......................................................... ...........................................i.............................N...... ......i.........................................N..............................N ................N.....i.....................................................N... ..N..........i...........................N............................N......... ...........................................................................N.... ........................................................................i.....i. ..........................................................................N..... ...N..........N..............N....................N................N............ ....N..............N....................................................N....... ................................................i....................N.......... ...............N................N...............N............................... .....................N.....................................................N.... ......................N...................................... Pos Score Pred ------------------------ 32 0.189 - 71 0.420 - 74 0.445 - 102 0.354 - 204 0.587 Yes 234 0.308 - 247 0.581 Yes 289 0.159 - 320 0.283 - 337 0.494 - 343 0.702 Yes 397 0.344 - 403 0.236 - 414 0.651 Yes 442 0.313 - 471 0.485 - 556 0.213 - 633 0.637 Yes 639 0.716 Yes 715 0.210 - 724 0.119 - 735 0.327 - 750 0.196 - 771 0.333 - 788 0.427 - 805 0.252 - 820 0.164 - 873 0.458 - 929 0.523 Yes 950 0.324 - 976 0.110 - 993 0.387 - 1009 0.129 - 1062 0.215 - 1116 0.072 - 1143 0.051 -

Explain the output -- Processing locus 743: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTATCCTACTTTGGTTTTTAATAAAATTTAGAGGACAACAAAAAAATAGTAAACTGCAAGCCCGACCCAGTAACCTATTA AGGTCCAAAACGACATCGTAGAACATAGCAAGTAAAAGAAGAAAAGATATAGAGATAGAGACAATGATATCATCATCATC ATCATCCAACATGGCATCATCATCAATCCTCATCCCTCCGATTCTCACCCGTCGTAATCTCCTCCTCTCCACCACAATCG CCACCGTAACTCCACCACCACCAGCTAAACCACCTTCCCCCGACATCACAATAACCGATCGAGTCTTCCTCGACTTCTCC CTCTGCCCCACCTACTTCCGCTCAGATCCCTCCGCGACACTCTCCTCCACCACACCTTGCTCCGATTCAACTCCTCTCGG CCGCGTCGTTCTCGGATTATACGGCCGTCACGTTCCCATAACCGTCTCAACTTTCAAACGTATGTGTACTTCATCTTCCA CATCTTACAAAAACACACCTGTCCACAAAATCTTCCCTGGTCAGTATTTTCTCGCCGGAAGACAAGGAGGAGGACGGCGA GATACGGCGGAGGTTGGTTATTCACTCAGAGACTTACCTCGTAACACCGACGTTGTTAATTCCAAAGCTTTTTTACTTCC TCATGCTCGTGCTGGTGTTGTTTCTCTTTGTTTATCTGAGAATGATGATGATGATGATATTAGACTCGATCCTGATTATA GAAATGTTGAATTCTTAATCACTACTGGTCCTGGACCGTCTCCGCAGCTCGATGGCGGTAACATTGTATTCGGAACTGTT CTTGAAGGATTAGATGTAGTGACGAGTATTTCTTCGATTCCGACGTATAAGCCGTCGGAGAATATTAAACAGTTTAACGA TTTTGCGGAGTTTCTTGGAGACGAGAGAGCTCAAAACGCGAGATCTTTGTGGAATAGACCTTTGAAGACGGTTTTCATTA GTGGCTGTGGAGAGCTTAAGGTCACTAATCCTTCACTCTCTCCAACTTTGCCTTAATTATCGTATCTTCGTATATATATA TATGTGTGTGTGTCAAATCTTGTTTATAATCATGCTTCTGCTTCTTCTACTATATGAAAATGAAATTGTAATTTA ................................................................................ ...............................................................i................ ..........i..................................................................... ................................................................................ ................................................................................ .............................................................i.................. ................................................................................ ................................................................................ ..N......................................N..N..N..N..N.......................... ...N...............................................N............................ .............N.................................................................. ................................................................................ ................................................................................ Pos Score Pred ------------------------ 144 0.745 Yes 171 0.640 Yes 462 0.700 Yes 643 0.074 - 682 0.254 - 685 0.228 - 688 0.224 - 691 0.286 - 694 0.140 - 724 0.138 - 772 0.134 - 814 0.136 - 1042 0.199 - 1072 0.055 - 1094 0.033 - 1100 0.071 -

Explain the output -- Processing locus 744: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATCTTCTTCGTCTCTTAAAACCTAAACCCTAATTGTCTTCTCCGGCGAATTCAATTTCCCGGGGCCACCGAGGTTCGT CGGAATTCTCTGTTTTCATAGTTTTCGATGTCGCAATTGATATTAATGTTCCAATTTAACTGTCAATTTCCCGTTTCAGG CTATTTCCGGTTTGTCGTTAGAATTCACCGATCGATGTCGCTCGTCCGTTGTTTATCCCTCAGATCGGTTCTCAATCCTC AACGTTACCGATCATCTTACTCCTTTCTCATCCATCTTCAGAATCCTCGTGAAGAAGAAGAACCTACACACAAAACTCGA AGTTACCACAGTATCATTCACCAGAACTCGTTAATCGGTGCCTCCTCGACGGCTTTCTCTCGCTATCGCTCTGTTTTCCA ATCTCCTGCGACGATTTCATCGGATCGATTCATTTCGACTTTCAATTTCTCTGGTTCCGGTGACGCTGTCATCGATTGGT TGTTGCAATCTGTGGTTTCGAGGAGTTATGAGTTACATAATGTTGCTGATGTTCTTGTTTCTCTTAACCATTTGCTTGCA TTGTTGAATTCTTTCACATTTTCTCAATGGTGGGTGTTTATCATCGTAGCATCGCTTTTGATTCGAGGAATCACAGTTCC GGTTATGGTTGATATGCTTAACAACATTGCAAAGTTTTTTAAGAGTCTACGCTCGAACCAAGGAGAGGTTCTAGACAAGG TATCATTTCTTAATAAAAGTCGAGGAGTAATGTACACCATGTTGGAGAAGGAGTTTTTTGGAGTTAAAGGATCAGTCATT GGTCAGGGTATTCAAGTTCCTATCTTCTGGTTCTCAATGGGAGAGTTGAAACAAAACATGGTTGAGATTCTTATGTCACG TCTTCGGGGTAAGATTTTGTCGGCGGAATTGATCAAGAACGGAGTCTTAAGCAGAGGACGCTTAGTGGTGGTCGTTGGCG ATGGATTCGGGATTGAAATCCAAATGTTCGATCTTGATTTCTCTCTTATCTTGAGGCGTCGTCAAGTCTCGCAAAGCTAA TAAGTTTCGATTTCATTCAATAATTGGAGCGTTTTGCTTTTAGTGGCTCAATGGTGTTTATTTGATGATAGAGCCAGCAC AAAATGTGAATAAAGCGTG ................................................................................ ...........................N.................N.................................. ..................................N............................................. ................................................................................ ................................................................................ ................................................................................ ...........................N...........N........N............................... ..........................N..................................................... ....N........N.................................................................. .............................N........N......................................... ....................................N....................i..............i....... ................................................................................ N......................N........................................................ ..................................................N.............N............... ...N............... Pos Score Pred ------------------------ 108 0.293 - 126 0.357 - 195 0.405 - 508 0.152 - 520 0.103 - 529 0.123 - 587 0.433 - 645 0.206 - 654 0.416 - 750 0.383 - 759 0.363 - 837 0.487 - 858 0.667 Yes 873 0.527 Yes 961 0.242 - 984 0.169 - 1091 0.063 - 1105 0.045 - 1124 0.056 -

Explain the output -- Processing locus 745: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTACTTAGCATGTTTTGTATCCAAGGGGTGTGACAAAATCGTTTAAAAGTTGCAGCGGATAAATTCTGAATAGAGCCAAT TTTTTCTGTTCTTGGTACTAAATCTTTGTCTCCATGTTTCATTGTGTATGTCAGGACTCAGGAATGAATCTTTCTCCGTT CAAGAGAGATATCGATGAGCTGATAGACGAGTTTGTGGAGGATGATTTGACAACCTTTGCTGATATGAAGAGTGTCTGGC TATCTAGAAAGTTTTCTCATATATATGAAGCTAGTCCTAACAGCAACCTAGCCTTTTTCATGCAGTCACTATATGTTCAT ACCATTGGTCACATGGTTAGTATTGATTCTTTTTCACGGAGACTTGGAGGTCTTTACTGTCTCTATTGCCTTCATGAGAT CCAACCCTTTAAACCCAAGTTCAGAATCTATATCTCACTTCAAGAGCTTGGGAAATTTAGGGATCTTGTAGTTGAGGCAA AAGATAAAGGTGTAGAGATAGCTGCTGCTGTGGCAAAAAAAATGCTTGATGAGAACATGTTAATATTTGGAGCTGTGGAT GAGACATCTGCAACCAAGACAATTCATCAATTAACCGAGCTACAGAACGCACGTGTGCGGTTTGCCTATGAGAAGTTAAT CTGTGACACTAGTATTAACCAATTTATTCATTTGGATATGGGTAAGGAAGTTGATCTGAATTCCCTAGATAAAATGTCTA TAGAATATGCAGAGGCCAAGAAGCGTGCAGTCGAAGGTGCAGGAGAAATAATGGAGATTGATGACATAAAGCATATATCA GAAGAGAAAGAGTTGATGGGAGAGAGAATGGAGAAATTGAAAGAGGAATGGGATTCACAAAGACTTTACTTTTATGAACA AACCAAGCTTGATGGTTTTACTACGACACCAAAACAGTTGACAAATGTGGAACATGATGAAGATGATGGGTTTGATGAGC TTGATCGCTTACTTTCTCAAAGCTAGTGTTGCCATTGCAATCACTGATCGATATCAGAGTCAGGTTTGTAGGAGAGAGCT ACACACACACTCTCACAAAATCAAGAGAGCATGTTTGATTAGGAG .........N...................................................................... .................................N.............N...............i................ ..............N..........................N......................N............... ........................N..................................N............N....... ............i............................................................N...... ................................................................................ .........................................N......N.......i.....................N. ...................................................................N............ .....................................i...................................i...... ......N...........................................N.........N................... ...............N...........i...................N.........................N...... ...........N................................i........i..i.....i..i........N..... ................................................................................ ..............................N.............. Pos Score Pred ------------------------ 10 0.306 - 114 0.249 - 128 0.480 - 144 0.699 Yes 175 0.126 - 202 0.229 - 225 0.262 - 265 0.152 - 300 0.169 - 313 0.370 - 333 0.523 Yes 394 0.087 - 522 0.359 - 529 0.134 - 537 0.505 Yes 559 0.117 - 628 0.104 - 678 0.779 Yes 714 0.746 Yes 727 0.214 - 771 0.324 - 781 0.160 - 816 0.467 - 828 0.520 Yes 848 0.294 - 874 0.088 - 892 0.169 - 925 0.795 Yes 934 0.822 Yes 937 0.709 Yes 943 0.631 Yes 946 0.625 Yes 955 0.193 - 1071 0.069 -

Explain the output -- Processing locus 746: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 747: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 748: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 749: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 750: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTTCAAAAAAATTCTATACGTGTTTAATTAAAGGACTATAGACTATAGTAATAGTAAATTAAGACTAATTACATATTTG ACCAAATCTACTTCAAACTGACCAATGTTTCACTACAAATACGTTCACAAACCATCATCATCTCTTCACCCCCTTCCTTC AAATATAGAAAATATGGCAACACAAAACAAGATTCAGAAAAACTCTCTCATCATTTTCCTCTTCACCTTAGTCGTCATCG CCCAGACCGCTACCTCTCAGAACTGCCTCACCATTGGTTGTCCTGGCATTAACGAGTGTTGTAGCCATACGGGTTACTGC GGCACCAATGTAGAGCACTGCGGCTTTTGGTGCCTCAGCGGCCCTTGCCAACTTTCTAAGAGTTCCAGCTCTTACAGACT CAATGATGGCCCACGCGGGAAGATCGAAAGCATTGTTACACCTGCATTATTCCACAGACTCATGCGTAAAGTCGGAAGTA ATTGCACGGGAAAAGGTTTCTACACCCGTGAGGCTTTCATCACAGCAGTCAAATCTTTCGAAGGCTACAAGGGAACGGTA GCTAAACGCGAGATTGCGGCCATATTGGCTCAGTTCTCTTACGAATCTGGAAATTTTTGTTACAAAGAAGAAGTAACGAG TGAGACATATTGCTCGTCAAGCAAGACATACCCTTGTCAATCGGGAAAGAAGTACTATGGTCGCGGTCTCTTGCAGTCAA TCAAATGGAACGAGTTCTATGGTGAGGCGGGAAAGTACCTTGGCCTCCCTCTATTGAAAGATCCAGATATGGTGGCTCGT AGTCCGGAAGTGGCTTTCAAATTTGCAATGTGGTTTTGGAAGACCGAAGTTGGTCCATCACTAAGACTAGGATTTGGAGC GACCACGATGAGGATCAATGGCATAGAGTGTGGTGGTATGAGTTGGAATGCTGAGGCTATGCAGAACAGGATTAACCAAT ACTTGGAGATCTGCAAGTGGTTTGGGGTGAATCCTGGTAAAGATCTATATTGCTAAAAGGCATTCCATGTGATAGTACGT TTAACTTAATTTAAGTTGTCGATTGTAATGTTTCCAATAATTAAAGGTTTCGATTGTGGTGTTTCCAGTTTTAAA ................................................................................ ........................i....................................................... .............i.................................................................. ................................................................................ .......i........................................................................ ..i..i.......................................................i.................. ................................................................................ ................................................................................ ........................................................N....................... ....N.............N.................................................i........... ...........................i.................................................... .......N.........N...................N.........N..........N..................... ..................................................................N............. ...........................N............................................... Pos Score Pred ------------------------ 105 0.559 Yes 174 0.679 Yes 328 0.607 Yes 403 0.609 Yes 406 0.683 Yes 462 0.674 Yes 697 0.454 - 725 0.275 - 739 0.362 - 789 0.651 Yes 828 0.535 Yes 888 0.377 - 898 0.435 - 918 0.330 - 928 0.308 - 939 0.177 - 1027 0.039 - 1068 0.096 -

Explain the output -- Processing locus 751: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATTGAAAAAGGTCAAAATATCAAATTTTATTTATTATAGAATGTATCAAAAACTTACAATAAAAAACTCTCTACCTGA GTTTTATCTCAATTTTATTAACCAATCGCATGCTTTCATAGAATGAGATATTATGAAACGACGTCGTTTAGGGTAAGAAC ATTACGTCACACGTTTATTTTAATATTATTTTATCATCGCCCTCTTTGTAATTTCGACTTCTCTTCTTCGTCAGATCTGA ATCTTAATTTCCCCCAAATCAAAGCTTTTGACCTAAATCCGTTTCCCGGGAAAAATAAATCAACAAACCATCACCGGAAT CTTCAATATCTCTCCGGTGAAGAAAAAATGTCGCACAACGATACGATTCCGCTTTATCAATCATCTCAATCAGACATTGA CGAGATTGAGAATATGATGAACGACAGTTTTCAATCAGGTCCCGGAACCGTGCTTCCTGCTCGACCACCGAGTCCGATCC GTCCGTCGATTCCCGTTTCATCCTCACCGTTCGTTCAATCCAATCTCCCACCGCTTCCACCGTCGTCTTCCTCCTCCACT CAGAAGGTGATGCCTGTTCCAGCTCCTCCACCACTTCCGTCAGCAGGTAACGAAGGTAACAAGAGTATTGGAGGTAGCGG ATTCGGATCTCCGCCAAATACATTGACGGAGCCTGTTTGGGATACTGTGAAGCGTGATCTGTCACGGATCGTGAGTAATT TGAAGCTTGTGGTGTTTCCTAATCCGTATAGGGAAGATCCTGGGAAAGCACTTAGGGATTGGGATCTATGGGGACCTTTT TTCTTCATTGTGTTCTTGGGGCTTACTCTTTCGTGGTCTGCTTCCGTTAAGAAGTCTGAAGTATTTGCCGTGGCATTTGC ACTACTTGCAGCTGGGGCAGTGATCCTCACACTAAATGTGCTGCTTCTGGGAGGACATATAATCTTTTTCCAAAGCCTAA GCCTTCTAGGCTACTGTCTATTTCCGCTAGACGTTGGAGCAGTGATCTGCATGTTGAAAGACAATGTGATACTGAAGATG GTCGTTGTGTCTGTGACTCTTGCCTGGAGCTCTTGGGCTGCTTATCCTTTCATGAGCGCCGCAGTGAACCCGAGAAGAAA AGCTCTCGCACTCTACCCGGTCTTCCTCATGTATGTCTCTGTTGGCTTCTTAATCATTGCCATTAATTGATTCTATCCGG AAAAATGGGAAATCAGAGATAGGTTCTTTGATTCAGTTTGGGAAGTCATTTGGTACAGAGTAGATAGAGAATTCTCCAGC CGCGAACTA ..........................................N..................................... .............................N............N.........N........................... ................................................................................ ................................................................................ ...........................i.................................................... .............N..i............................................................... ................................................................................ .........i...................................................................... ................................................................................ ...................................................................N............ ................................................................................ ...................................N............................................ ..................................................i............i.............i.. ...................................................N............................ ............................N...N............................................... ....N........................................................................... ......... Pos Score Pred ------------------------ 43 0.314 - 110 0.404 - 123 0.243 - 133 0.121 - 348 0.889 Yes 414 0.488 - 417 0.565 Yes 570 0.698 Yes 788 0.350 - 916 0.174 - 1011 0.653 Yes 1024 0.529 Yes 1038 0.537 Yes 1092 0.293 - 1149 0.327 - 1153 0.173 - 1205 0.237 -

Explain the output -- Processing locus 752: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTCTGTGATTCTTGTGCATTCGAAGGTATTTCACTTTTGTTGTTGATGCTTCTAAATCCAATTCACATCCATGTAACCC TAGTTTTGTTATAATTAGTGATTGAAATTAGTGTATTTAGTATCAATCTGGTATTTTTATCCATCCCGGGATGTCTTGTA TATGTTATCCCTGAGTTCTCTAACATCTATCATAACTTCAAATCTCTGCTTTGTTGTAGCCATGGCTGAACGAGAAGACG CAGCAGAGGTTTCTCTTTCAGTGAGAAACGAGGAAGATCTTTCTCCTGAATGTGTAGCTTGGGCTGATTCTTGCATCATC AGTTTTCTAGATGATTCAGACAACAATAATTGGGGAACTTTTAGAGATGCTTTAACAGAAATCATCGATATCCATCCCGA GATGTTTGTTTTTTCATCAACAACGGGAACAAGAGGTGTCCTGTCTCCAGACGAGGTTATGACTGAATCCGAGACTATTG ATCTACGGAGATCTGAACCAGCAGCTGGTTCAGCGAACAGTAGAACTAATTCATCGAATGAAGAAGTTAGCGAGATTATC TCTATGCTAACTTTTGAATCAGACCCGTCAAAGAATTGCTTAGAAGATTATTATTTTCCAGAGTCTATAGCAGAGAATGG AAACCGTGAACCAGCGGACGGTTCTAAGACAGATCTTGGAGGTGTTGAAAGTATAGAAGAAGATGGGTCAGTGAGCAATG GAGAGGCTAAAGAAGAAAAACCAGCGTCAGTATCGTCTCAAGTATTCAAGGATGATTTCATGAGCACATACGTTGAAGAC AATGCTGAGGATTGTAATGTTACAGAGGATCCTGTCAAAGTAACTTCACAAGAGATTTTCAAAGTGTGGGATCTAGAGGT TGTGGGGGACAATGATGAGGAAGATGGGTTGGTGCTGCAGCTGAAGAAGGCCCTCGATGAGTCTTCCACTGTCCAGCCCC TTCCACAGCCACTGAATGATGATCAAGTTGTATCAGAGAAGAGCAATATCGATGATCTGATATCTGGAATATCGGATCTG TCTCTTGCAGAAACTTTTAAGTGAACTATGTGTATGTGTTTTATTTTGACATCACAAACTCTCTTTTGCTTTACTACTCC TTAACCGACACAAACCGGGTTTGAGATTTCAAAATCTGCTACT ..............................................N........................N........ ......................................................................N......... .................................................N.............................. ..........N...................................N................................. .........................................................N...................... ...N........................................................................N... ..............................................................N..............N.. ...................................................N.......N.................... ...........N..N........N................................N....................... ...............N..N................................N............................ ...........................N.....N.............................................. ........................................... Pos Score Pred ------------------------ 47 0.150 - 72 0.367 - 151 0.373 - 162 0.707 Yes 222 0.691 Yes 290 0.189 - 331 0.240 - 367 0.429 - 402 0.788 Yes 459 0.616 Yes 538 0.148 - 564 0.274 - 637 0.478 - 703 0.206 - 718 0.223 - 772 0.215 - 780 0.391 - 802 0.238 - 817 0.116 - 892 0.256 - 895 0.202 - 904 0.144 - 937 0.109 - 976 0.071 - 979 0.155 - 1012 0.135 - 1068 0.278 - 1074 0.109 -

Explain the output -- Processing locus 753: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACGCCGTCTTCCAGAACAAACATCACTTTGAGGGCAAGGTATAATAAAGTTCACATCTTTAAGCTTAACAAGTGTTGGA AGACTTTCTATTTGTTTGTAGTCGATGAAAGATTATCTATTGCGAAGTTGTACCCATTGGTCTCTCTGTCTTTCTTGTGG TATTAATAGTTTGAATTGTTGTTTCTGTAATGTGATGAAGACTGTTTTAGACGTGGGCACCGGGAGTGGAATTCTTGCAA TTTGGTCAGCTCAAGCTGGTGCCAGGAAGGTTTATGCCGTTGAAGCTACTAAGATGGCTGATCATGCTCGTGCCCTTGTC AAAGCCAATAACCTTGACCATATTGTAGAAGTCATTGAAGGATCTGTCGAGGATATTTCATTGCCTGAGAAAGTTGATGT AATTATCTCGGAGTGGATGGGATACTTCCTTCTGCGGGAGTCCATGTTTGATTCTGTGATTAGTGCTCGTGACCGATGGT TAAAGCCAACAGGTGTCATGTACCCTAGTCATGCTCGCATGTGGCTTGCACCCATCAAGTCCAATATAGCAGATCGGAAG AGGAATGATTTCGATGGGGCAATGGCAGACTGGCATAACTTTTCGGATGAGATCAAAAGTTACTATGGGGTTGATATGGG AGTTTTAACAAAACCTTTTGCTGAAGAGCAAGAAAAGTATTACATACAGACTGCTATGTGGAATGACTTGAATCCACAAC AAATCATAGGCACTCCCACAATTGTCAAGGAGATGGATTGTTTGACAGCATCTGTCAGTGAAATCGAGGAAGTCAGATCA AATGTAACGTCAGTTATCAATATGGAGCATACAAGGCTATGTGGTTTTGGTGGATGGTTCGATGTTCAGTTCTCGGGGAG AAAAGAAGACCCCGCACAGCAAGAAATCGAGTTGACCACAGCTCCAAGTGAGCAGCACTGCACGCACTGGGGACAACAGG TTTTCATTATGTCGAATCCTATAAATGTTGAGGAAGGTGATAATCTGAATCTTGGTTTGTTAATGAGCCGGTCCAAGGAA AATCACCGACTAATGGAGATTGAGCTTAACTGTGAAATAAAAGAAGCATCTGGTAACCCCAAAGAATCTTTCAAGAAGAC TTACTTCATAGAGTGATTGATTGATTATAGAGAAGCTTTCCTTGGGGCTTGCTGGTTCTTGTATCCCATGTATGAACCCC AAATGTAATGTTTTATTGCTTCTTGCCTTTTTTTT ................................................................................ ........................N....................................................... .............................N....i............................................. .................................N...................i.........N................ ............................................................................N... ................N..........................N...............................N.... .................i............N.......i......................................... ....N........N.......i........................N.................N..........i.... .......................................................i......N................. ................................N............................................... ................................................................................ ........N...............N.....................................i................. ............N................................................................... ...................................................................N...N........ ..N....N........................... Pos Score Pred ------------------------ 105 0.232 - 190 0.205 - 195 0.662 Yes 274 0.087 - 294 0.827 Yes 304 0.110 - 397 0.210 - 417 0.260 - 444 0.314 - 476 0.489 - 498 0.790 Yes 511 0.211 - 519 0.550 Yes 565 0.161 - 574 0.417 - 582 0.693 Yes 607 0.122 - 625 0.222 - 636 0.667 Yes 696 0.730 Yes 703 0.192 - 753 0.444 - 802 0.497 - 822 0.549 Yes 839 0.664 Yes 854 0.574 Yes 862 0.352 - 969 0.236 - 985 0.278 - 1023 0.569 Yes 1053 0.331 - 1188 0.050 - 1192 0.037 - 1203 0.067 - 1208 0.033 -

Explain the output -- Processing locus 754: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGTGTGATTGTAATTGTTTGAACTCAGTCACACCTTCAAGCTTTTACTGTTTCGTCGGCCCTCTTGTTTCCACAAAGTT CCTCCCGAGTCTCCTACATAAACATCAGTCTCAATGCTAAAAATTTATTTTAAAACACCCCACTTGGAAACACAAATAAA TAAAAATAAATTCAAAACAATGTATTCTTCATCATCTGTATCAAAACGCTTCGTTCTAGTCCCTATTGTGGTCGTGGTGA CCACACAACTCCTCCTGGTACGTAACGTTTCTTCACTAAACCTTACCAATTCGTATCTTCACCACAAATGCGTAGTCAAT CAAGGGAAATACAAGCCAGGAAGCAAGTACGAGAAATCCCTCGATGATATCATCCAAAGCTTTTCGAATAAAGATAAAGA TTCGTATGGTTTTCGTACCGGTTACAGCATGAAGGCCTACGGTAAAGAACCTGATATGGTCTCCATCACCTACCAGTGCC GTATCGACTCTCGTGGGCCCAAGTGCCAGTCCTGCGTTGTCACGGCCGGTTATGAGCTTCTTCGTAAGAGATGTCCGAGA TATAAAGAGGCAATAATATGGTACGACCAATGTCTTGTGGAGTTTTCTTCGTTAGATACTTCTGGGCAGATCAATTACGA CGACAACTTCTGTATGCCTAGCGCGAAGAACCTGATCGGCAATTCAATCTCTCTTGAAGAAAGGTTGCATCTCCTCAACA ATCTTACAAAGATAGCCGTCACGAAAATAGATAAAAACATAGAAGGTATAAAAAAACCAGTACTGTATGCGGCGGGGGAG AAGAGACTCGGGACGAAAAGTCTATACGGAATGGTGCAATGTTCGGCTGACTTATCTGTTCAGGGTTGTAATGAGTGTAT GTTATATTATATAGTGCATTTTCAAGAGTGCTGGGAAAATAAACAAGGAGTAAGAGTTTTGAGTAGAAGCTGTAACTTTA GGTATGAGCTTTACCCTTTTATTAACCCTAAGGGTCCTTATTATACAAAGTTCTAAAGCAATTTGAATATGTTACTCTTA TAATGTTTTTTCTTTTCTTTTTAACTTCAAATTTCCCCTTACTTAACGTGAATTGTTTTCTATTATTTAGTAAAA ................................................................................ .................................N.............................................. ...................i............................................................ ...................................................................i............ ...........................................N.................................... .....N......................i..........................i........................ ...................................................N..................i......... .................N...........i.................................................. .............i.................................................................. ..................................................................N............. ..............................N.......N...............................N.......N. ................................................................................ ...N................................................................N........... ..N........................................................................ Pos Score Pred ------------------------ 114 0.430 - 180 0.823 Yes 308 0.619 Yes 364 0.269 - 406 0.424 - 429 0.774 Yes 456 0.722 Yes 532 0.460 - 551 0.611 Yes 578 0.350 - 590 0.559 Yes 654 0.503 Yes 787 0.350 - 831 0.131 - 839 0.126 - 871 0.286 - 879 0.078 - 964 0.256 - 1029 0.149 - 1043 0.054 -

Explain the output -- Processing locus 755: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGCTGATTTGAGTTCTTTTTTAAAAATCATTAGCTAAGTTAGTTTCAGAGCTCTTCTTCGAATATCTGGAAGAGAGACGA GTTTAGGTCGATCGATTTTGAAAATGGTATAGAGCTTCGTTTATTTGTGAGAACGACCAAGCTTTTTCTATTGTGATGTT ATGGTATAATGATATGCATAGAATTCTACGTGAACCAGGAATGGATCAATATGGGATTCCAGTAGAGAGAAAAGAGATGA AGCATAAGGGGAGAAATGTTATATGGTCTGTTGGAATGGATAAGTGTTTGATTGAAGCTTTGGCTGTCCAGGCCAAAAAC GGGAACAAAGTTGACAAATGTTTTAACGATAAGGCTTACACTGCTGCTTGTGTTGCGGTTAATACTCGTTTCAATCTTAA CTTGACTAGCCAGAAAGCCATCAATCGCCTCAAGACGATCAAGAAAAGGTACAGAGTTATGAGAGACATACTTAGCCGGG ATGGATTCTGGTGGAACTCGAGCACAAAAATGATCGATTGCGAAAGTGATGAGCTCTGGAGAAGATATATTGCTGTAAAC CCAGACGCAAAAGCATTTAGGGGAAAGCAAATTGAGATGTACGAAGAACTTAGAACAGTATGCGGTGACTATCAAACTCC AGGAAGTTCTGAAGAACATAGCGATACAGATGGAACAGAGTCATACGCAGGAGCAAGTGAGTATATGCATGAAGAGTCTC AAGATCTTCCTCCTCCTCGAGATCCTTTAAGACGACCTTCTAAACGTTCTCGAAACTCGGATCCTTGTCAAGAAGCAATG TTAGTGGTTGCTTCTAGCATTCGCCGCCTAGCTGATGCAGTGGTTCAAAGCAAAACTTTGATTAACACAGAGGAATTGTT GAAAGCAGTGATGGAGATTGATGAATTGGAAGAAGCAAAACAGATGTATGCGTTTGAGTATCTGAATGGTGATCCAGTCA AAGCTAGAGCTTTCATGGCTTATAATAATAGGATGAGGAAGATGTTTTTGTTTCGACAGTTTTGGTGGTGGAAGTAAAAA AATTGATTTGCTGCTATGGCTAAAAGAATCCTCCTCTTCTTTTGGGGTAATGTAGAGTCTTGTTAATGTTAAAAGCTGGA AATATTTGTTGAGGCC ................................................................................ .......................N...................................................i.... N.......N....N..........................N.........N.........................i... ...............N......N............i............................................ .................N.............................................................. ..........................................................i..................... N............................i..................N............................... ....................................i......................N.................... .............................N..................................i...i........... .............................................................................i.. ..................................N............................................. ..........i.........N......................N...N.................N.............. ..............N.................N........N...................................... ...............N.................................N...............N.............. ................ Pos Score Pred ------------------------ 104 0.499 - 156 0.619 Yes 161 0.332 - 169 0.178 - 174 0.364 - 201 0.483 - 211 0.366 - 237 0.704 Yes 256 0.160 - 263 0.167 - 276 0.753 Yes 338 0.442 - 459 0.711 Yes 481 0.212 - 510 0.716 Yes 529 0.317 - 597 0.594 Yes 620 0.179 - 670 0.333 - 705 0.509 Yes 709 0.566 Yes 798 0.796 Yes 835 0.130 - 891 0.644 Yes 901 0.247 - 924 0.379 - 928 0.151 - 946 0.166 - 975 0.388 - 993 0.130 - 1002 0.180 - 1056 0.219 - 1090 0.072 - 1106 0.050 -

Explain the output -- Processing locus 756: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACGAGTGATCAATCTATTTAGCAATTTAAAAACTTTCAGATTTATATAGAATTTGTTTTGTCTGTTACTTATTTAGGA CAAATCTTTAGTGTAAATTGAGTTGAGAGTTTTGTTTTTTCTTCTCTCGTAATGATGACTTGCCGTTTGGCCAAAAGTTA TGGTTTAATCAAATTGTTGTTGTGTAATAAGAATAATGGCCGTCGTCTTCGTTTTCCTCCGGCTGATGGGTTCTCTGCGT TTCACAGTTGCTCCACCACGAGGAACAGATCCATGTCTGCTTCTAGTTCCTCCACAAATCCCATGTCCCGTGAGGACGCA ACAACACTGCTTCCGTCTGTTCAAGACAAGTACGGTGGCGTGATGACCGAGATGACTCATCCCATGGATCCTTCTCTCTT CTCCACACTTCTCCGATCCTCTCTATCTACCTGGACTCTTCAGGGCAAAAAGGGAGTGTGGATCAAGTTGCCAAAGCAGC TTATTGGTCTTGCTGAAACTGCTGTTAAGGAGGGATTCTGGTTTCACCACGCGGAGAAAGACTACTTGATGCTTGTCTAT TGGATTCCCAAAGAAGACGATACGCTTCCTGCCAATGCATCTCACCGTGTTGGCATAGGTGCCTTTGTCATTAACCACAA CAAAGAGGTGCTGGTGGTTCAAGAGAAGACAGGGAGATTTCAAGGTCAAGGTATCTGGAAGTTCCCCACCGGAGTTGTGA ATGAGGGTGAAGACATTCATGATGGCTCAGTCAGAGAGGTGAAAGAAGAGACCGGAGTGGATACAGAATTCGACCAAATA TTGGCCTTCAGACAAACACACAAAGCGTTCTTTGGAAAGTCAGATTTGTTCTTCGTGTGCATGTTAAAGCCCCTTTCTTT GGAGATCAACGCACAGGAGTCAGAGATTGAGGCAGCTCAGTGGATGCCTTGGGAGGAATACATAAATCAACCATTTGTAC AAAATTATGAGCTATTGAGGTATATGACAGACATTTGCTCTGCTAAAACCAACGGAGACTACGAAGGCTTCACTCCTCTC CGCGTCTCTGCACCTGATCAACAAGGCAACTTGTACTACAACACCCGCGACTTACATTCGCGCAATTGACCTTCTTTCTC AACAAACAGAGCTTTCCTCACAGATTCTAACGAAGCTGGTTTCTTTGCTGCCTCTCTATGTAAGCATTTTGACACTTGAA TCACTACA ................................................................................ ...................................................N..N........................i ...................................i.............................i.............. ................................i.............................i................. ..........................................i........i...........N................ ................................................................................ ....................................................................i........... ..................................N............................................. ................................................................................ N.................N..N.......................................................... ............................................................N................... ...........................................i.................................... ......N................i........................................................ ................................................................................ .........................................................N...................... ........ Pos Score Pred ------------------------ 132 0.397 - 135 0.240 - 160 0.505 Yes 196 0.699 Yes 226 0.542 Yes 273 0.745 Yes 303 0.775 Yes 363 0.585 Yes 372 0.559 Yes 384 0.296 - 549 0.686 Yes 595 0.153 - 721 0.417 - 739 0.182 - 742 0.299 - 861 0.432 - 924 0.570 Yes 967 0.427 - 984 0.691 Yes 1178 0.065 -

Explain the output -- Processing locus 757: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACATTTCTAAAGCCTCTTTTAATCATGTCTTAAATATATATACCCCTATCCATAGCATAGCTTGCCCCTACTCATAACCT CTAAACTCTTTAGACAACTAAATATATAATATAATGTCTGAACTTCATTCTTCTCTGCAGCTTCCTGTGTTAGACTTAAC ACAACCAATTGAGTCCTCTATACTGTCTTCTCTCTCTGAGGCTTGTAAAGAGTGGGGATTCTTCTATGTCACCAATCATG GAATCTCTAAAGAGATGTTCAGCAAAATTTGTTCCTTGTCCAGAGATGTTTTCAAAGCTCCTCTTGAATCAAAGCTCAAG CTTGGTCCCATTTCATATACTCCTCGGTACATCGCCTCTCCTTATTTCGAAAGCCTTGTTGTCTCTGGACCTGATTTCTC TGATTCTGCCAAAGCTTCAGCAGATGTTTTGTTCCAAGATCATCATAAACCAGAGTTAAGAGAAACAATGCAAGAATACG GAGCTAAAATGGCAGAATTGTCCAAAAGACTCATAAAGATTCTCTTGATGATGACTTTAGGAGATGAAACCGGAAAGAGA CTCTACCAAACTGACTTTAGCAACTGTCATGGATACTTAAGGCTAGTGAACTACACACCTCCACACGACGTGGAGAAGCA AGAAGAGCTCGTTGAAGGTCTCGGGATGCACACTGATATGAGCTGCATAACAATAGTCTATCAAGATTCCGTTGGTGGGC TTCAGATGAGGTCTAAAGAAGGGAAATGGATCGATATAAACCCGTGCAACGATTTCCTTGTTGTTAATATTGGAGACCTT ATGCAAGCATGGAGTAATGGACGGCTGAGATCATCTGAACATCGAGTTGTATTGAGAAAGTTGGTGAACAGAGTCTCTCT GGCGTTTTTTCTCTGTTTTGAGGATGAGAAAGTGATACTTGCACCTCAAGAAATTGTGGGTGAGGGAAAACAAAGAAGCT ACAAGTCGTTTAAGTGTTCGGAGTACCTGAAGTTCAGACAGAGCAATGAAGAAGGGAAGTTTGAGAAGATTGGTTTCACT GTCAAAGACTTTGCTGGACTAAAGCTGGCTCAACCAGATGATCCTTAGAGACCAAACTGGTGTTTCGGTTTAGAGATCAC TAAACCGGTTTTAGAAGAAGAAAGATATTGTTGCGAGTGTTAACTAGTGGAATCAAAAGCTTTTGGA ........................N....................................................... .................................i.............................................. .................................................................N...........N.. ..............i..............................N.................................. ................................................................................ .......................N...........................................i............ ........N......................................N..N............i................ ............................N................................................... .........................N...........N.......................................... .....i...................i...................................................... N.......N.......N............................................................... .......................N........................................................ .............................................N.................................. .....................................N.......................................... ................................................................... Pos Score Pred ------------------------ 25 0.401 - 114 0.809 Yes 226 0.240 - 238 0.171 - 255 0.661 Yes 286 0.375 - 424 0.218 - 468 0.768 Yes 489 0.476 - 528 0.261 - 531 0.246 - 544 0.500 Yes 589 0.408 - 666 0.313 - 678 0.145 - 726 0.638 Yes 746 0.774 Yes 801 0.089 - 809 0.459 - 817 0.321 - 904 0.273 - 1006 0.283 - 1078 0.172 -

Explain the output -- Processing locus 758: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGACCTAGTGAACTCTTAGTAACGTTTTAGGGTTTTAACTTTTTTTCTTGGATGAATGATGCTGGGTCCTTCTTTATG TTAGAGTTGTTCTTTGTTTAAAGAAGTTATCAACGTCAGTTTGCTTTAGTTTATAAAGCTTCTCGTTCGACGATTTTTCC TCTGCAACTAGAAATGGTGGTTCTGTTTATGTCAATACTAGAAACCGTGTATATGCTTAGCTACTTAGGTCTTGTTTTGG TTTTGCTTTATAGATATCTTGTTTCTGAATGTTTTCTTCTGCTTGATTGTGCTGGATTTGTGAATGTCTTCTTTTGTTTG TTTCTACTTAAAACCAGGAGTTCATCGCAGATGGAGTCATTGTTAGAATGTTTTGCAATCACGGATGGCAAATGTCATCC CGATTGCTTAAAAGCAAACAATGAGCAAGAAGATTACGATGCATGTCAATCTGCAGCTTTGGTAGCTGTGAGTTTGATTA GCTCTGCACGTGTTATCTTCAAGATCGACTCTAAGTATACTGAGTACTCACCTCAGTATTTGGTGGATAACGTTGGGAAG GAAGAAGTTGAGGGAGAAATGGATCAACCAAGCTGTCAGTACACTGTCGGAAACCTCCTTAGTTACTTGGTGGAAAACGT TTGGACCAAGAAGGAAGTTAGGCAGAGAGAAATGGATCAACAACGCCGTGAGTTCACTGTCAAAGACTGCTTTGAATTTG CTTTTAAAAAAGGGCTTCCAAGAAATGGACATTGGGCGCATGTGGGATGTATATTCCCGGTTCCTCCATTTGCTTGTCAA ATACCTCGCGTTCCCATGAAAGGAGAAGTGATTGAGGCTGCAAATGTGAGTGAAGCGTTGAAGCTGGGTATGCAACAACC AGCGGCAGCAAGGCTGCATTTGTTCAGTCCAGAGTTTGATCTTGTTGGAGAGGGTATTTACGATGGCCCGTCAGGTAATG AAACACGATATGTTGGACTTAGAGATGTGCTCATGGTTGAGGCGGAGAAGATCAAGGGAGAAACTGTTTTTACTGTGCAG ATATGCTACAAGAAGAAGACTTCATTTGTCAAAGTGTCTACGAGAAGTATGATTCTCCCGCTTAATGGTGACGACGAGTC TCAGGTCACAGAGCCAGCATGTCTACTTGTTGACTTCTGTATCCCACGTTTTTCTATCAACTAACCAAGTGAGCTGAGAT CTTGGTAAGTATCTGGAGAAGTTTATGCTAGTTTGTCGTTCCTATATTAAGAATGTTTTATCAGACTTATATTTTATGTT TAA .....................................................N...N..N................N.. ................................................................................ .............N..............N.......................N........................... ............................N..................................N................ ..............................i................N................N......N........ ....................N.................N...N..................................... ................................................................................ ..................i............................................................. ...............................i................................................ ........................i..............N......N................................. ...............N...........................N.........................N.......... ..............................................................N..............N.. .........N..............N.......N............................................... ..i.............................................N...............N............... ..................N............................................................. ........................N...........................N......................N.... ... Pos Score Pred ------------------------ 54 0.261 - 58 0.090 - 61 0.165 - 78 0.141 - 174 0.222 - 189 0.107 - 213 0.445 - 269 0.406 - 304 0.468 - 351 0.809 Yes 368 0.176 - 385 0.496 - 392 0.269 - 421 0.237 - 439 0.248 - 443 0.168 - 579 0.803 Yes 672 0.631 Yes 745 0.507 Yes 760 0.247 - 767 0.454 - 816 0.406 - 844 0.315 - 870 0.383 - 943 0.156 - 958 0.066 - 970 0.157 - 985 0.172 - 993 0.390 - 1043 0.673 Yes 1089 0.392 - 1105 0.381 - 1139 0.437 - 1225 0.048 - 1253 0.058 - 1276 0.037 -

Explain the output -- Processing locus 759: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATCAAACCCAAGATCGTGACTATTTCAAATCTTAACATAAGATATAAATCTTCGTTTCTACTTTTTTTTCTTCCATAAT ATAGTCAATTCGTTTTCTTAATTAGGGCTTCTTCTCTTTGTTTCTCCAATCTTTATTAGTTTATTTATTTATTTTGGTTA TTGTATACAAATGGCAATGGCTTTAAACATGAATGCTTACGTAGACGAGTTCATGGAAGCTCTTGAACCATTCATGAAGG TAACTTCATCTTCTTCTACTTCGAATTCATCAAATCCAAAACCATTAACTCCTAATTTCATCCCTAATAATGACCAAGTC TTACCGGTATCTAACCAAACCGGTCCGATTGGGCTAAACCAGCTCACTCCAACACAAATCCTCCAAATTCAGACAGAGTT ACATCTCCGGCAAAACCAATCTCGTCGTCGCGCTGGTAGTCATCTTCTCACCGCTAAACCAACCTCAATGAAGAAAATCG ACGTAGCAACTAAACCGGTTAAACTATACCGAGGCGTAAGACAGAGGCAATGGGGTAAATGGGTAGCTGAGATTCGGCTA CCTAAAAACCGAACCCGGTTATGGCTCGGTACGTTCGAAACGGCTCAAGAAGCTGCATTAGCTTACGATCAAGCAGCTCA TAAGATCAGAGGAGACAACGCTCGTCTCAATTTCCCAGACATTGTTCGTCAAGGACACTATAAACAGATATTGTCTCCGT CTATCAACGCAAAGATCGAATCCATCTGCAATAGTTCTGATCTTCCACTGCCTCAGATCGAGAAACAGAACAAAACAGAG GAGGTGCTCTCTGGTTTTTCCAAACCGGAGAAAGAACCGGAATTTGGGGAGATATACGGATGCGGATACTCGGGCTCATC TCCTGAGTCGGATATAACGTTGTTGGATTTCTCAAGCGACTGTGTGAAAGAAGATGAGAGTTTCTTGATGGGTTTGCACA AGTATCCTTCTTTGGAGATTGATTGGGACGCTATAGAGAAACTCTTCTGAATCCATTTTATCTTTTTGATTCATTTGTCT CTAAATTGTAGAATTTTATTTTCAGAGCTTTGTAAGGGAAGTTCTTGAATGAGAGTTGCAGAGGACTAG ................................................................................ ................................................................................ ..........i.....i...........N...N...................N....................N...... .....................................................................i.......... ................................................................................ ...................................................................N............ .................................................i........i..................... ....................N........................................................... ................................................................................ ................................................................................ ...........................................................i.................... .....................................................N.............N............ ................................................................................ ................................................N.................... Pos Score Pred ------------------------ 171 0.779 Yes 177 0.812 Yes 189 0.390 - 193 0.406 - 213 0.434 - 234 0.233 - 310 0.660 Yes 468 0.449 - 530 0.567 Yes 539 0.678 Yes 581 0.366 - 860 0.659 Yes 934 0.167 - 948 0.249 - 1089 0.094 -

Explain the output -- Processing locus 760: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTTTTTCCCTTCAATTATATACTTTGTTTAGATTAATATTTAGGATGGAATAAGATCCTGGCTTCGTGGCTTGTAGTG CTGATTAAAAATCACTGATAACCAATGCAACGCCGGTTCGATACTTCACAAAATTTAGGGTTCTTTAAAAAATTCATAAA CTCCAATTCGCCTCCTTCTCTCTTTCTCGATCCTCTCAAAATCTCTCCACCACCAATGAAGCGACGACGCCGTAACTCCA AACTTAAATTCGAAGAAACCTCCGACCAATCACCGACATCCACGGCAGAGTTCTCCTTCTCTCTCACCAAAATCGCCGTC AGCCAAATCTGCCAATCTATTGGATACAAAGCCACCGATGCCTCCGCTCTCAACACTCTAACCCTAACCACCACCAAATT CTTACAATCCCTCGCCGAACTCGCATCGTCGTTCTCTAACACCGCTAATCGCACGGAGGTTAATCTCTTCGACATCGTCA ATGGTCTCCAAGATATCGCTTTATCTACTTCCGATTGTTTCCCCGGTGGCTCTACGGTTCACGATATTGAATCTCAGTGC CTGATCAAATCCGCTGTTCTCCGTAACCTCTCCGATTTCGTTACCTACGCTCCTGAGATTCCTTTCGCTAAACCGTTACC GAGACGGGAAAGAGACGGATCGTTCGGAGGAGATTTGGATCACGTGGCGGTGACTCGATCGGTGGATGTGACGTCAGTTC CAGCTTGGCTTCCGCCTTTTCCTGACTCTAGTCTCTGCTCCGATCGTTGTTCAAAGGATAATAGATCTGATCACTTGTGG GAAAACTCTGATTCCGTTATTCGCCGTGAAATTTTGCCGGAGAGTTTGAAATCTAAAAGCGGAGGAAGATTACCGGTAAT GAGAGATAAAGTGAGATTCAAGATGGAACAACGAGATTGGAGTAGTGGTGGTGATACGAGATTGGAGCGGTCGCGGGACA ATAACAATGGCGAATCTGGCCGTGATCTTGAGGTTAAGAAGAAAATTAGAGAAGGATACACTTACTGTGCCGGCGAGTGG CCGTTTTAGTCTATTCTGATGACGTGGAAAAAAACAAAATCGCGAGGTTTAAGATGGATTCGAACCCGCGAGGCTTGAGT ATATGTGTGATTGGCCAATTGGAATCTA ..............................................i................................. ........................N....................................................... .......................................................i........................ ................................................................................ .....................................i.......................................... ................................................................................ N............................................................................... ................................................................................ .................................................................N.............. ................................................................................ ..............................................................................i. ......................i......................................................... ......i......................................................................... ..................N..................................N.......................... ..N......................... Pos Score Pred ------------------------ 47 0.652 Yes 105 0.245 - 216 0.502 Yes 358 0.627 Yes 481 0.409 - 706 0.153 - 879 0.786 Yes 903 0.751 Yes 967 0.792 Yes 1059 0.130 - 1094 0.067 - 1123 0.073 -

Explain the output -- Processing locus 761: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGCCCATTACGACATTAAGAGACCGTTTCCATAATCCGAAACCCTAGTTTGCTTCTGTCTTTAGTTGTTGCCTTTTAAA CTAAACTAACGAACTTAATAAGCGGCATATCTAAATCTGAGAAAAATGGCGATTGATACGATACAAAAAACAAAAAAGCT TCGGTGGGGAGAAATTGACGAAGAAGAAGGCGACTATGATTTCCTTCTTCCGCCTAAGCAGATGATTAGTCCGGACCAGA ACGGTGTAAAGAAGGTGATTGAGTACAAATTCAACGAAGAGGACAAAAAGGTCAAAATCACTACCACGACCCGTGTTCAG AAGCGAGCTCTCACCAAACAAGCCGTGGAGCGACGGAGCTGGAATAAGTTCGGAGACGCAGCTCATGAAGAATCCAGTAG TTACCTCACAATGCGTTCAACAGAGGATATCATCTTGGAACGAATTAGAGCTCCTGGTAGCAACGCGGAACAGTCGACCG TATCAGGAGATAGCATGTCTCAGTTGGGCAAACCGGGTGCGGTCCTCATGGTCTGCAGGTTATGCCAAAAGAAAGGTGAC CACTGGACATCAAGATGCCCACAGAAGGATCTCTTATCCTTAATGGACGAGCCTTTAACAGCAGAAACTTCTACATCGAC CATAACTGGAACTGGCAGGGCGGCTTATGTCCCGCCAAGCATGAGAGAAGGTGCAGACAGAAAAGCTGGTGGTTCAGACA TGAGGAGTAGACATGATGAGAACTCTGTCCGTGTGACTAATTTGTCTGAAGATACCCGTGGACCAGATTTGATGGAGTTG TTTCGTCCGTTTGGTGCTGTAACCCGTTGTCATGTAGCCATTGATCAGAAGACAAGCATGAGCAGAGGATTTGGTTTTGT CAGTTTTGTGAGCAGGGAAGATGCACAGCGAGCAATCAACAAATTGAATGGCTATGGTTATGATAACCTCATTCTTAGGG TTGAATGGTCCACTCCTAAAACCCAATTAGATCCGTTTTCATTCGCTGATTATAATAATCTTTGTAAGTTGTTGAATATA TGTCATTCATGATTATAAGTTCTCGCGATACATAGTAATTCATGTTGGGGTCTCGTATGCAAGTTCCTACTAAATGTATT TCGGTTGAATACGTGCAAAAAAAGTAATAAA ................................................................................ .............................................i.................................. ...................................N.........................i.................. ................................................................................ ................................................................N............... ..........i..................................................................... ..............N................................N.............N.................. ..............i...........................i..................................... ..........................N.............i......................................i ............N..N.......................................................N........ ...............................N.........................i...................... ....................N..........................N.....N.....N.................... ....N..........................................................................N ........N................................N..............N................N...... ............................... Pos Score Pred ------------------------ 126 0.615 Yes 196 0.142 - 222 0.667 Yes 385 0.316 - 411 0.793 Yes 495 0.382 - 528 0.262 - 542 0.442 - 575 0.709 Yes 603 0.580 Yes 667 0.412 - 681 0.693 Yes 720 0.524 Yes 733 0.200 - 736 0.089 - 792 0.324 - 832 0.377 - 858 0.685 Yes 901 0.269 - 928 0.124 - 934 0.127 - 940 0.090 - 965 0.221 - 1040 0.473 - 1049 0.124 - 1082 0.078 - 1097 0.047 - 1114 0.059 -

Explain the output -- Processing locus 762: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGTAGTGGATGTGACATTCTAGAAGCATGTTCCACTTCCCTCCTTTGTCTTCCACCATCTCACTCTTCACAAAAAAAA AAAAACAAAGCTGAAAAATAAGTGAGAACATCAAATACCAAAAATACCCCCAGTTCCCACATGTGCTTCTATTTGCTTTC CGAAAAAAACCAAACGCACCCATCTCCTTCCTCTTCTCCTTTTCTCTCCGAACAAAGAACACAACCAAAACAAAAATCTA TGTTAGATATGTCGGAATCTGTACTCACGGCGATGTATAGTTGGTTCACTCCGACAGTTCTCTTCGTCTTCCTCAACCTT ATGATTGGTACCATCGCCATTAGCTCATCTTTCTCTTCCAAATCCAACGACCCAAATCAAACTCAGATCCAACGCTCTCC TTCCATGATTCATCGTCTCAAATCCATAAACTTCTCTTCTTTCACTTCTCCAGACAAGTCTCATCTCGAGTTTCCTCCTT CAACACCTGAAGACAACAGCAACAACAACTTTCACCAACCAGCTTCAATCGAACAGAATCAACCATTCTTGTCTAGATCT CCTTCTGTTCTTCACAGAATCAAATCTTTCAATCTTTATAACTACATTTCTCAAGAACCCACCAATATAATCGAAGCTTC ACCACCGTCGGTGACCGTAGAGACAAAACAAGAACAAGTACAAGAACAAGAAGTAAAAGAAGAACAAGAAGAAGAAGAAC AGAGTCTTGAAGAGGTTTATAGTAAACTTAATCTAAACCATGTCGCTAGGACAAAATCTGACACTGAACCAGCTGCTGGA ATTAGACCTCCGAAGCTTCCAAAGAAGATGAAAAAATCTGCTAGTACCAAATCTCCGTTCTCTCACTTTCAAGAAGATGA AATCTCCGTGGAGGCTCGCCGTCCTGCGACGGTGAAAGTGCCGAGAGTTACGACGGTGGAAGAAGCTGATGAAGAAGTAG ATGCAAAAGCTGATGACTTTATCAATCGATTCAAGCATCAGTTGAAGTTACAGAGAATTGATTCCATCACTAAGTATAAG GAGATGGTGAAGAAAAGAAACGACAAGTGAAAAATTGATAAAAAGAAAACTCTTGAGGCTGTTTCTTAACTATGTATTAA TTTATGGACCTTTTTGAAAATTCTTAATTTTGGTTAGCTTTTTTCAATT ..........N.................i................................................... ............................................................N................... ...............................................................................N ........i.......................i............................................... N............................................................................... ....N........................................................................... ................................................................................ ................................................................................ ................................................................................ .......................................N........................................ ...........................i................................................N... ....................................................................N........... N...........N................................................................... ...N...................................................................N........ ...N............................................. Pos Score Pred ------------------------ 11 0.344 - 29 0.531 Yes 141 0.406 - 240 0.446 - 249 0.538 Yes 273 0.720 Yes 321 0.288 - 405 0.491 - 760 0.288 - 828 0.594 Yes 877 0.361 - 949 0.122 - 961 0.108 - 973 0.110 - 1044 0.383 - 1112 0.074 - 1124 0.055 -

Explain the output -- Processing locus 763: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGATTTCTTAATTAGCTCTAAAAGATTAAGATCACAGGGGTCCATTGTCAAAATTCTCTTTTGATTTTGTTTTTGTAATC AAGAATAGTTTCTTTTTTTGATTCATGAAGAAATGTCTAATTTTTTCGTTGCAGAGAGAGATGGGTATATATCTAAGTAC TCCAAAAACAGACAAGTTCTCAGAAGATGGCGAAAATCATAAACTCAGATATGGTTTATCCTCTATGCAAGGTTGGCGTG CGTCCATGGAAGATGCTCATGCTGCAATACTTGATCTCGATGATAACACTTCCTTCTTGGGTGTCTATGATGGTCATGGA GGTAAAGTTGTTTCTAAGTTCTGTGCCAAGTATCTACACCAGCAGGTTCTTAGTGATGAGGCGTATGCAGCTGGAGACGT AGGGACTTCTCTTCAAAAAGCATTTTTCAGAATGGATGAGATGATGCAAGGACAAAGAGGGTGGCGAGAGTTAGCAGTAC TTGGTGACAAAATCAATAAGTTCAGTGGGATGATTGAAGGGCTTATATGGTCACCAAGAAGTGGGGACAGTGCTAATAAA CCTGATGCTTGGGCGTTTGAGGAAGGTCCTCATTCTGATTTTGCTGGACCTAATTCTGGGAGCACGGCATGCGTAGCTGT TGTTAGAGACAAGCAGCTATTTGTTGCAAATGCAGGTGACTCACGTTGTGTGATATCCAGAAAGAATCAGGCTTATAATC TTTCTAGAGATCACAAACCAGATCTTGAAGCTGAGAAAGAAAGGATATTGAAAGCTGGTGGCTTTATACATGCAGGGCGA GTCAATGGAAGCTTAAATCTATCACGAGCTATCGGTGACATGGAATTCAAGCAGAATAAGTTTTTGCCATCTGAAAAGCA AATAGTTACCGCTAGTCCAGATGTTAACACTGTTGAACTCTGTGATGATGATGATTTCCTTGTTCTTGCCTGCGATGGAA TTTGGTAAACTTCAGCTTGAAAAAGTTTTGAAGTTCATTTCTTGGTAGTTATATTGATACACCTGTCTTGGTTCATCTCT TATCTGCAGGGATTGCATGACAAGCCA ................................................................................ ........................N.......N...........................i................... ..........................i.......................N.............i............... .....i......N.....N....................N..........................N..N.....N.... .......................................................N........N............... ...............................i...N....N..N.................................... .............................i................N................................. ....N...............................................................N........... .............................N.................................................. .....................................................................N.......... ....N..................................i........................................ ....................N.......................N..N..N.......................N..... ................................................................................ ................N.......... Pos Score Pred ------------------------ 105 0.140 - 113 0.172 - 141 0.650 Yes 187 0.700 Yes 211 0.353 - 225 0.728 Yes 246 0.703 Yes 253 0.204 - 259 0.152 - 280 0.115 - 307 0.232 - 310 0.145 - 316 0.274 - 376 0.252 - 385 0.342 - 432 0.580 Yes 436 0.225 - 441 0.354 - 444 0.439 - 510 0.529 Yes 527 0.148 - 565 0.154 - 629 0.189 - 670 0.284 - 790 0.464 - 805 0.184 - 840 0.531 Yes 901 0.310 - 925 0.167 - 928 0.149 - 931 0.110 - 955 0.088 - 1057 0.045 -

Explain the output -- Processing locus 764: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCCCTAATCTTAGATCCCCTGCTTAGGGTTTCGAAATTTGTCTATTTTGTTCAGCTTTATACTGATTCTATTTGTCCA ATTTTGAAGATTTGATTCTAAGTCTCTAGTATATTCTTTGTTATGCAATGCAGAAACATGTCTAATTCAAAGGACGAGAA ATCACAAGATGCAGCTGATAGAATCAAGGCTGCAACACTCACTGCTGCTAAAGGTCTAAGCCGTACACAAGCGGAGAGAG CAGCGGCTGCAGCAGCGAGAAACGTTAACGCTTATGGCCAGAAAGAAGAAGGTCCGAGTAGATGGCAGGAGAAAAGAGAA TTAAAGAGGCAGATGTATTTGATGAGTACTGAGAAAGCTGTCAGGCTTGGTGAAAGGAAAGATAAAACAATGTCAGCTTC AGCCGTGGGAAGCAGTGCTTCGGCGGCTTCACAGTGTCAGAAATGTTTCCAAGCTGGGCATTGGACTTATGAGTGTAAGA ATGAAAGGGTTTATATCTCGAGGCCTTCGAGAACTCAGCAGCTTAAGAATCCTAAACTGAGAACGAAGCCTTCTGTTGAT GATCTTGATGGTAGTGATGATGATGATGAAGAAGAAAGGCCTGATGCAACTAATGGGAAAGCAGAAGTTGAGAAGCGATC TAAGAAGAGTAAGCGAAAGCATAGGTCAAAGTCTGATTCTGAAAGTGATAGTGAAGCCTCTGTGTTTGAAACAGATAGTG ATGGCTCATCTGGAGAGAGTAGTTCAGAATACAGTTCATCGTCGGACTCAGAGGACGAAAGGAGGAGGAGAAGAAAGGCC AAGAAGAGCAAGAAGAAGCAAAAGCAGAGGAAAGAAAGGCGAAGAAGGTACAGCTCTTCTTCATCGGAGTCATCTGAGTC AGAATCAGCTTCGGACTCAGATTCTGATGAAGATAGAAGCAGAAGAAAGAAGAAGAGCAAGAGGCACAGCAACAAGCGTC GTTGAATTGGTTTTAGGAAATCGATAAGTGGATGAAAATTTTCATGCCTTAGAATCATTTTAAGCTTCTTAAAGGAAACT CACTGCTATGTTACTTCTTTTTCT ................................................................................ ..........................................i....N.........i...................... ........N....................................................................... .................................N...........................i.................. ............i........N...............................................i.......... ..........................................N.........................N........... N.............................................................................N. .......N........N..N..N..N.................N........N........................... ................................................................................ N............................................................................... ................................................................................ ..........................N..................................................... ...............................N...........N.................................... .......N................ Pos Score Pred ------------------------ 123 0.631 Yes 128 0.337 - 138 0.857 Yes 169 0.430 - 274 0.158 - 302 0.585 Yes 333 0.781 Yes 342 0.364 - 390 0.719 Yes 443 0.348 - 469 0.168 - 481 0.230 - 559 0.328 - 568 0.198 - 577 0.178 - 580 0.101 - 583 0.339 - 586 0.167 - 604 0.054 - 613 0.151 - 721 0.321 - 907 0.310 - 992 0.037 - 1004 0.042 - 1048 0.056 -

Explain the output -- Processing locus 765: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACAAGTTAAATTTGTTAATAATAAAACGACTTGACTTTACCATATATCTTTTTCTTTTTTAAATTAGAAACTTTTAAGA AGAAGAATACAAAAGAAGTAGTGGCACTTTATGACATTTTCCACAAAGGTTAAAAAAATCACATTCCTTTATAAACAACG ATCCATTAGTGTCGGAAAACGAAAGAGAAAGAAGAAGAAAGAAACAATGGAGTCTCCCAAATCTAACGTCGACGATGGCT ACCAAAACATCTTCGTCATGCGTCACGGTGATCGAATCGACAATTTCGAGCCACTCTGGGTTTCAACCGCCGAGAGACCT TGGGATCCACCTTTGGTTCAAGACGGTATGATTCGAGCGTTTCGAACCGGTCAAAGGATCCGATCTCAGATCGGGTTCCC GATTCACCGTGTCTTCGTCTCCCCTTTCCTCCGATGTCTTCAGACAGCTTCCGAAGTCGTCGCAGCTCTATCAGCCGTCA ATGTCGATCCTAACGCCATGTCTTCCAAAGACGTCCCTTCCATTGATAAATCTAAGCTCAAGGTTTCTATTGAATTAGGT TTGTGTGAGATGTTGAACTCAGTGGCTATAAGGCGTGAGCTTGCTCCTAAAGATGGGAAATTTGACTTCACTGTTTCAGA TATTGAAACTATGTTTCCTGAGGGAATGGTTGATCATAATGTGGATATGGTTTATAAAGAGTTGCCTAAATGGGAAGAGT CTGTGGAAGGTTGCAGAGATCGATATGTTAAAGTTGTAAAGGCTCTTGCTGATAAGTATCCTGAAGAGAACTTGCTCTTA GTAACCCACGGGGAAGGAGTTGGAACTACATTTTCGACATTCTATAAAGATACAACAGTGTACGAAGTAGACTACTGTGC TTATGTTGAAATGAGAAGACAAGTCTCAAGTGAAGATGGGTCTGTTAAAGCTGGGGACTATGAGGTGGTTTTGAGTCATG GTCAAGCTGGGATCAGGTTTAGTCATGATCCTGTCAGTTATGAACCTGTCATAAGCCATTCACCTGTTTAGTCAAATTGT CTTCAGTGTCCTCTGTTTCTGTACTTTTGAATTGTATTTGAACTGCTTTTGTATGAATTATGAAACATTGTTTCAAAGTT TTCATTCAAT ................................................................................ ..............................N................................................. ..............................................i...........................i..... .................i.............................................................. ...........................N.................................................... .................................N.............................................. N................i.............................................................. .........N..........................................N........................... ..........i..............i............N.......i......................N.......... ........................N....................................................... ................................................................................ ..N.......i........................N.......................N.................N.. ........................N..............N........................................ ....................................................N......N.................... .......... Pos Score Pred ------------------------ 111 0.370 - 207 0.836 Yes 235 0.618 Yes 258 0.588 Yes 348 0.249 - 434 0.112 - 481 0.362 - 498 0.669 Yes 570 0.397 - 613 0.279 - 651 0.578 Yes 666 0.597 Yes 679 0.073 - 687 0.657 Yes 710 0.429 - 745 0.093 - 883 0.162 - 891 0.614 Yes 916 0.269 - 940 0.294 - 958 0.170 - 985 0.149 - 1000 0.199 - 1093 0.060 - 1100 0.037 -

Explain the output -- Processing locus 766: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGGTATAATGAGATTCTTTTCTTGATTTCATTTATAAGATTCTTCTTCTCTAGAAGCTTAGACAAAAAAAAACTTCAGAT TCTACGAACTATAGTGGTTGAAGTAATTTATTGAATAGAAATTTTGCATATAGTGTTTTTGCAATCAGAGATATGGTAGG AAGAAGCTATAGCTTAGAAACGCGTGGATTTGAGTCCACCACAGATCTTAGAATAGCTCTTTATGAAATGAAAGAAGATG TGCAGAGACTTGAGGAAGAGTTGGATGCTGAGAGAGAAGCTACTGCTACGTCAGCGAGTGAAGCCATGTCTGTGATACTT AGGCTACAAGGCGAGAAAGCGTTGCTTGCTATGGAAGCTAGCCAGTACAAGAGAATGGTTGAGGAGAGAATGTCTCATGT TGAGTTGTCAATGGAGCTTTTGGAGGATTTGAATTACCAGAAAGAAGTTGAAATCAAGAATCTTGAGTGTGACTTACATG CTTATAGATGTAAGCTTATGAGTTTGGGATGGAGTGGACTTGATGATGAGGATTGTATTAGATTTTGCGATAGATCTCAA ACACCGTCTCCAGAACCGAATGAGACGGTTCTTGTGGAGAAGGGTGTTATAGAGCAGAGCTTGGATTCAAGAAGAGATCA TGAGAAGAATTTAGATTTGAATTGGGAAAAGATCAAGAAGGTGGATGAGCAGTTGAAAGAGCTTACAGATTTTCGAGATT CTGTAAGAGATCAATACAAGATTTTGAAGCAGGAGACAACTTCTGTTTCTGAAACTAAGAATGGGGAAAAAGGTATGTGT AAACCTGATTTGTTGGTGAAAAAGATGTCCAAGAAATCATTGAAACAGAAAAGAGATAAAAGTATCAAGAGAGATCAAGC ACTAGGAAGTTGTTCTGCAAATGATGCAGAGTATCAAGCCGAGTTACAGCGGTTGAGAGAGCGAGTAGAGCGACTAGAGC AGGAGAGATGCAACAAAGAGCCAGCTCAAACTAGTGGAGTAAGTCAAGAAAATATGAATCTGCAGAGAAAATCAGAAGAA GAGTTAAGTTCTATGCAATCTGCAATGTTTAGTTATGACTCAGCTATTGTTTCTGTTCAAGAGGCAATGCTTTACTTCTG GCTATGAGACACCCTCTCGTCCAGAGTTGACGGCTTTAGAGCGAGTTATGATATCTACAAATAGCGAGTTGATTTTATAG AAAAGTATACAAACCTTGAGAACCTT .......N........................................................................ ........................................................................i....... ..............................................................N....i.........N.. ........................N........................................i.............. ..............................i.......................i..............i......N... ..........N..................................................................N.. .......N.........N..........N.............N..N.................................. ...................N...........................................................N ............................................N................................... ............................................................N.............N..... ........................i....................................................... ....................N..N........................................................ .......N.............................................N.......................... ............N...........i.........N...............................N............. ...N...........................................N................................ .......................... Pos Score Pred ------------------------ 8 0.133 - 153 0.617 Yes 223 0.092 - 228 0.537 Yes 238 0.339 - 265 0.411 - 306 0.691 Yes 351 0.541 Yes 375 0.556 Yes 390 0.592 Yes 397 0.122 - 411 0.372 - 478 0.174 - 488 0.434 - 498 0.411 - 509 0.355 - 523 0.214 - 526 0.091 - 580 0.077 - 640 0.215 - 685 0.144 - 781 0.449 - 795 0.370 - 825 0.515 Yes 901 0.249 - 904 0.345 - 968 0.221 - 1014 0.369 - 1053 0.462 - 1065 0.511 Yes 1075 0.413 - 1107 0.266 - 1124 0.104 - 1168 0.057 -

Explain the output -- Processing locus 767: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTATTTATGTTACAAAATACAGTAGTTAACGTTTTTTTTCTTAAAAGTAGTTTTTTTTTTTTTTTCATATTAAAATGG GCACTAAGCCCGTGTGTCTAAATGGGCCGTATCTATTACTTTTGGATCCGAACCCGTTTTAAAAAACACAATAAACAATC GGGTAACCCGTTTTTTTTTTTTTTCCTGATTTCTCTACGTAAACCCGGTTTGACCAAACCGCGTTATAGCAGAACCAAGG AAAAGGAAAGAGTCGGCGAAAAAGATATAAAAAAGCCAGCGAGAGAGACAAAGGTCAGAAGATTTGATTTTTCTTATTCC TCTTCAAAGGCAAACAATTTACACAGCTATATATATATTTTTAGGGTTTTTGAGTATCTCGCGAGGTTTTACATAGATTT CTCCATTATGGATTCGATGATGAATCAGAAGACTGTTTTGGCTGTGACGGATGATGTGGTGTTACCTGTTAGCTCTGTTT TAGCTATAATGAAAGAGCTTGGAAAGGAAGTGATTGAGAGTTTTGATCCTCTGATTATTACTCAAGCATCCACAATAAAT CAGTTTCCTTTGGATGCTTCCTCTGTGGAGGCGGTATTAGCCATTTCCAAAACATCTGATTTCCCAAGTGATAAAATATG TGGTGAGTTCTCAAGAATCTTGAAGCCTGGCGGCACGGTTTCTGTGTGCAAAGTTTTGGAGGGTGAAACGGGAGAAATTC AACAGACCATTCAAAGGAGAGTGACATTGGCTGGTTTTCTTGAGCCACAGTGTCTTGATTTGAAATCAATTAAATTGTCT ACGTTCAGTTTATCCTTTGGGATTAAGGCAAAGAAACCTTCTTGGAAGATTGGTTCATCATTTGCTCTCAAAAAGCCTGT AACAAATCTCTTTAAGATTGATCTAGATGACGACGTGGATCTTATTGATGAAGATTCTCTTTTAACAGAGGAGGACTTGA TGAAGCCACAGCTTCCTGTTGCCAGCGGTTGTGAGACCACTAAAAAAGCTTGCAAGAACTGCGTCTGTGGTAGAGCTGAA ATTGAGGAGAAAGCTGTGAAGCTGGGCCTCACAGAGGATCAAATCGAGAATCCACAATCATCATGCGGCAGCTGTGGGCT CGGTGATGCCTTCCGCTGCGGTACATGCCCTTACAAGGGTCTGCCACCCTTCAAATTAGGCGAGAAGGTAACCCTATCTC AAAACTTCCTTGAAGCTGACATATAAGCATGTGAAAGGCATCTTCATACATGGGTGTTTCTCGCCGAGGCCAAAACCTTT TGCTAATCGCAGAGTAGTTTCATCATTTACTTTGTGCCCTTTCGT ........N...................................................................N... .....................N.......................................................... ................................................................................ ................................................................................ ................................................................................ .......i........i..i..............................N..N.......................... ........N....................................................................... .............N...............................................................i.. ................................................................................ ................................................................................ ................................................................................ ..........................N....................N...............................i ................................................................................ ..............................................................N................. .....N..................N....................................................... ............................N....................N.............................. ............................................. Pos Score Pred ------------------------ 9 0.136 - 77 0.232 - 102 0.292 - 408 0.665 Yes 417 0.602 Yes 420 0.712 Yes 451 0.096 - 454 0.163 - 489 0.324 - 574 0.164 - 638 0.660 Yes 907 0.220 - 928 0.208 - 960 0.693 Yes 1103 0.392 - 1126 0.341 - 1145 0.379 - 1229 0.205 - 1250 0.288 -

Explain the output -- Processing locus 768: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCTTGATATCTAATCTAATTAAGGTGTAAACTATATGAAAAATTATACCAAAAACTATAGAAAAAGTTTCACTTAACGT GAAAGAAGCATCGTTTGATGAATGAAAACACATGAGAGCATGTATAAACGAACCTTCAGACCAATGAAAAAAAAGTCCAC CGTTAAAGTCAAAAGTCAACGCGTTGATCATTTACACATCTATATAACAAACTCATCTTCTTACACCAAAACCATCAAGA TCGATCCCTCTCTAAAAATGGCTCAACAACACTCTTTTCTCCTCTTATGCTTCTTCCTCTCAATCTCTTATTTACTATCT TCCGCCCAAACCGAAGCCACCTCAATCGAGAGATTAGTGCCAAGAGACCTCTACAACAAAATTTTCATCCACAAGGACAA TACCGCTTGTCCCGCAAATGGTTTCTACACTTACGAATCATTTGTCCAAGCGACGAGGCGGTTTCCGAGATTCGGGAGCG TAGGAAGCCCCGTGACACAACGACTCGAAGTGGCTGCATTTTTGGCTCAGATTTCACACGAGACGACCGGAGGATGGGCA ACGGCACCAGACGGACCGTACGCTTGGGGATTATGCTTTAAAGAAGAAGTGAGTCCACAAAGCACTTATTGTGATTCTTC TGATACACAGTGGCCTTGTTTCCCTAACAAGACTTATCAAGGAAGAGGTCCAATTCAACTATCGTGGAACTACAATTACG GACCGGCCGGTCGAGCCTTAGGATTCGACGGTCTAAGGAATCCAGAAACGGTGTCAAATAATTCAGTGATAGCTTTTCAA ACAGCACTTTGGTTTTGGATGACGCCACAGAGTCCCAAACCTTCGTGCCATGACGTCATGATCGGAAAATACCGACCTAC GGCGGCGGATTTGGCAGCTAACCGGACCGGCGGGTTTGGTTTAACCACAAATATAATAAATGGCGGTTTAGAGTGTGGGA TTCCTGGAGACGGACGGGTCAATGACCGAATCGGGTTTTTTCAGAGATATACTGGGCTGTTTAAAGTAGCAACTGGGCCG AATTTGGACTGTGAAAATCAAAGGCCTTATGCTTAAACAATTAATTTGGTTGACCCGAAATATGTATGTTTATAAACAAA AAGTCAACATGTATGTTTATGGCATTGCCCACACATGGAGCCATAAAAGGGGTTT ...................................N............................................ .................N...N.........N.......N.......................N................ ................................................................................ .................i............................N................................. ................................................................................ .................i.............................................................. .........................................................................N...... ................................N............................................... ................................................................................ ................................................................................ ..................i..............................N.......N...................... ...........................................................i.................... .....................N.......................................................... ............................N................................N...N.............. ........N...N.....N...............N.................... Pos Score Pred ------------------------ 36 0.301 - 98 0.229 - 102 0.298 - 112 0.290 - 120 0.331 - 144 0.391 - 258 0.786 Yes 287 0.393 - 418 0.677 Yes 554 0.494 - 593 0.246 - 819 0.718 Yes 850 0.345 - 858 0.273 - 940 0.734 Yes 982 0.279 - 1069 0.355 - 1102 0.114 - 1106 0.108 - 1129 0.139 - 1133 0.064 - 1139 0.058 - 1155 0.077 -

Explain the output -- Processing locus 769: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTTTAATTTTCAGGGGCAAATTGGTCACAAGTTTAATGAAAAAGTTGGAAAAGTGCCTCATCCCTGACAAGTGCCTC AAAAGGAATAAAATTCTCTAAAAGTGCCTAAACGTGTCACATTCCCTTAAAGAATTACGAAAACAAATACACGGGCGTAA AATGCCTAAATTAATTATCGTAAAATCATATAGAATCGAAGAAGAAGATTCATATAATTATAATCCAATCCAGGTCTTAA TTTACGCTTTCACTCGCCATTATTGTTCCCTCTTCTTCTTCTTCTTATTCTCTGGATCTCTCCTTCTCCCTCTCTTCTTT CTACAGTTATTTCGCTTTAGCAGATTACTTAAATCCACGGTTTCTTCTTCTCCGGGAGCATTTATGGCGTCAGCGGTGAC GGCGTCAGGCAAGGTGGAGAAAGGACATCAGCTATATAGAGACGGCAAGTATAAGGAGGCGCTTCTCTTCTATACGGAGG CTCTTACTGCTGCTAAAGCTAAACCACAGAAGATCGCTCTTCACAGCAACCGCGCCGCTTGTTACTTGAAATTGCACGAT TTCATTAAGGCAGCAGAAGAATGCACTTGTGTTCTTGAGCTTGATCAGAAACATAGCGGAGCGTTGATGCTGAGGGCTCA GACTTTAGTCACTCTTAAAGAATACCAATCTGCTCTTTTTGATGTCACCAGGCTTATGGAGTTGAACCCTGATTCTGAGG TTTATCAGAACCTCGAAGCTCGCTTGAGGACGCAGTTGTCCCTTGCTCCAATTCCTGAGTCTGAAGCAGAATTGGAAGAA GAGAGTGACGTTGAACAAGACGCTGAAGATAAAGAAAGTAGAGAAGTTGAGCTTGGTGTCAATGAGAGAAGAGATAAGAG GTTTGAGTCGGTAGTTTCCCTTAGAAGAGATCTAGAGACTACAGGAGAAGATGCGATAAACAAGGGGGAAGTTGTTGCAC CTAAAACACCAGAGGTTAGAGAACAGAACAGCAAGGAAGTGCCAATGTCTGGAAAGCAATCTAATGCATGGCAAGCCATA CCTAAGCCTAAGGGTCATTCAACACTCGATTACGCCCGTTGGAATACAGTTGAAGATGATTCCAGTGAAGAAGAAGACGA TGAAGACAGCGATGACAGTGATGAGTCGCCACCTCAGTACAGATTCCGTGTTAAGACAGTCGGGGTGCGTCCTGTGAAGT GAAACATTGTAGATAGGAACATGGGATGGTTTCTTTCATCCTGATATATTGCATATTGCACAGGAGAGCTGTGAAAAAAG CATTAGAGTGAAGGGGATAGT ......................................N......................................... ................................................................................ ................................................................................ ...............................................................i................ ................................................................................ ................................................................................ ....................N.............................................i............. .........................................N.............i........................ ................................................................................ .............................................................N.................. ..................................................N............................. ............................................i..................N...N............ .......................................................N.......................i ...........N........N........................................................... ....................N....N...................................................... ..................... Pos Score Pred ------------------------ 39 0.325 - 162 0.549 Yes 384 0.593 Yes 581 0.178 - 627 0.848 Yes 682 0.139 - 696 0.572 Yes 862 0.161 - 931 0.210 - 1005 0.640 Yes 1024 0.348 - 1028 0.356 - 1096 0.281 - 1120 0.575 Yes 1132 0.367 - 1141 0.205 - 1221 0.137 - 1226 0.131 -

Explain the output -- Processing locus 770: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGCCATAATCACTACGTATTGTTCACTTCAAAAATTTAACATGGGGTGCCAATTGTCACATGTAAAACTATATGGTCCT AAAATTAAAAATATCAAGTAATTAAGGATAATAATGAAACATGTGTGTGCACGTGTGATGGAGAGAAACAAACAAATCAG CTTTAGAATTCAGATTCCTTCTTGTCACACAGTTTTCGTTTCTCTTTCTCTTTCTCTCACACTCTCTGCCTATCTCCGCC GCGGAGATCCATCGTGTTTTCTACTTCTGTTGGTATCCATGGATTCTCTCGTTGAATCCGGTTGGAAGTACCTTGTTACG CATTTCAGTGACTTTCAACTGGCGTGTATTGGGAGTTTTATACTTCATGAAAGCGTGTTTTTCTTGTCTGGACTCCCTTA CATTTTCCTAGAAAGGACTGGTTTTCTGAGCAACTACAAAATTCAGACCAAAAGTAATACTCCTGAAGCCCAGGGAAAAT GCATTGCTCGACTATTACTTTACCATTGCTGCGTAAACTTGCCCCTCATGATGGCGTCGTATCCTGTATTCAGATTCATG GGCATGGAAAGCAGTTTTCCTCTGCCGTCCTGGAAAGTGGTGTCTGCCCAGATCTTATTCTACTTCATCATTGAGGATTT TGTATTCTATTGGGGTCACAGGATCTTGCATACTAAATGGCTCTACAAGAACGTGCACAGTGTGCATCATGAGTACGCGA CACCGTTTGGTTTGACATCAGAATATGCTCATCCCGCTGAAATTCTGTTCCTTGGTTTTGCTACCATTGTTGGTCCGGCT CTCACCGGGCCTCACCTGATCACCCTTTGGTTATGGATGATGCTCAGAGTTATTGAGACAGTTGAGGCACATTGTGGTTA TCATTTCCCATGGAGCCCCTCGAATTTTCTTCCTCTATACGGCGGATCTTTAATATTAATGTGGGAATCATTTGCTTACA GTGCTGACTTCCATGACTACCATCATCGATTACTCTACACAAAGTCTGGCAACTACTCATCAACGTTTGTTTACATGGAC TGGATCTTTGGCACCGATAAAGGTTACAGAAAACTTAAGGCCCTAAAAGAAACCTGACAACAAACAAACGTGAAATTCGA TTCCCGAGAGAGGTCTTGTTCTCAAAAAACCTTCTTTCTTTTGTTTTGTCTCTACCATTTCCTCAACTTTAGTCAT .........................................i..................N...........N....... .................................N......N................i...................... ................................................................................ ......................................i......................................... ..............................................N................................. ..............................................................................i. ...............................................N..N..........................N.. ...N............................................................................ ....................................N...............................N........... ........................N....................................................... ................................N...N..N........................................ .........N................................................i..................... ............N.............................................................i..... ................................................................................ ............................................................................ Pos Score Pred ------------------------ 42 0.531 Yes 61 0.167 - 73 0.168 - 114 0.133 - 121 0.219 - 138 0.522 Yes 279 0.660 Yes 367 0.369 - 479 0.586 Yes 528 0.391 - 531 0.296 - 558 0.421 - 564 0.322 - 677 0.444 - 709 0.170 - 745 0.271 - 833 0.298 - 837 0.107 - 840 0.320 - 890 0.413 - 939 0.524 Yes 973 0.368 - 1035 0.588 Yes

Explain the output -- Processing locus 771: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTTTTATGAACAATCGAACCAAACACAAAACCCGGTTAACTGTAAGAGGTTTAATTTGAACCGGATATAAACCGGTTG AACCAGACACTCCCACCTTAACTCTTTTTTCCTGAGGTTTGGTTGCTCAAAGCAAACAAATTCTCAATCTTTCGTTTTTC ACCTTCTTCTTCTTCTTCTTCTTCAATGGCGTTGATACATGGTTCGGTTCCTGGTACGTCTGCGGTTCGATTGGTTTTTT CAACCTCAGCGTCACCGTCGAGATTTTGTCTCAATGTTCCGGTGGTGAAGCAGGGTTGGAAGAATTCGTGCCGGAGAAGG GTTTTAAGAGCAATGGTGCAGGAAACAGTTCAAGGATCTCCTTTGGTTTACGCCAGAGAAATGGAGCGCCTTTCTGCTAA GGAATCGCTACTTCTCGCTGAACAGAGCTCTGTGTGTTTGGAACTGTCAAACTATTTTTGTCCTGAAGCCATCTATGTAC AGTTGAAGGATGCTGGAGGCTTTGAGGCTTTAGTTACAGGGAAAACAACTAATATGCAAAGGATCGATGTTAATGAGAGG ATTACGAGTCTAGAGCGGCTTAATCCAACTCCGAGACCAACCACGTCTCCTTGTTTCGAAGGCAGGTGGAATTTTGAGTG GTTTGGATCTGGAAGTCCTGGTTTACTCGCTGCTCGAGTTATATTCGAGAGGTTTCCTTCAACATTGGCGAATTTATCAA GAATGGAGATACTGATCAAGGATGCTAACGCAAAGGCGACTGCAAATATCAAACTTTTGAACTCGATAGAAAGCAAAATC ATTCTGTCATCAAAATTGACTGTTGAGGGACCACTGAGATTGAAAGAAGAATATGTTGAAGGTATGCTTGAAACTCCAAC AGTCATTGAAGAAGCAGTTCCTGAACAGCTAAAAAGCGCTTTGGGACAAGCTGCCACAACGTTGCAGCAGCTTCCTGCTC TTATCAAGGATACTCTAGCCAGTGGTCTGAGAATTCCCCTTAGTGGATCCTTTGAAAGATTTTTCATGATATCTTATCTA GATGAAGAGATTCTTATAGTAAGAGATACCGAAGGAGTGCCTGAAGTTCTAACGAGGATAGAAACACCATCATCGACCGT TGTAGAAACTATCGAATATGATAGTTAGATGTGACTAGCCATTGTTCTTGTTCTTGTTCCTATGTTCTCTGTGTGTTATA TTACAAGAACCAACCACCAAGTTGGTGTCTGTAAATGTATGACGTTA .......N........................................................................ ................................................................................ .........................i............N......................................... .................................N.............................................. ............N...............................................i................... ..........................................................................N..... .........N...........................................N............N.....N....... ................................................................................ ................................................................................ ..N..................N.......................................................... ....................................................N..........N................ ................................................................................ .................................................................N.............. .................N..........N................................N.................. ..................................N...N........ Pos Score Pred ------------------------ 8 0.195 - 186 0.818 Yes 199 0.347 - 274 0.353 - 333 0.436 - 381 0.587 Yes 475 0.171 - 490 0.324 - 534 0.418 - 547 0.164 - 553 0.459 - 723 0.454 - 742 0.116 - 853 0.426 - 864 0.476 - 1026 0.488 - 1042 0.207 - 1138 0.210 - 1149 0.203 - 1182 0.032 - 1235 0.062 - 1239 0.049 -

Explain the output -- Processing locus 772: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCAAAAATAAAAGTTAATAAGAAAGGGTAAACAGATGTCAAATCGGTTTCATGAGTGAAACTAACATAAAAGAAAAAGC TAGACTTTCAAAAGCATATAAATAGATAACTCTCTAAAGCTTTTAAGCCAGTGGTTTATTCATAAGCACCATCTTCTCTA TATCTCGTGTTTTGAATATCACCACATTTTCATAATGGATTCAGCAAAAAAATCAGATTTCTTTTCAAATTTACATGATT CTCTTTTAGTGTTTATAATATCAATGCTACCTTTCAAAGATTTGGTACGAACGAGTGTTCTTTCTAGACGTTGGAGGTAT CTTTGTCGTGAAACGACAAGCCTCGTTTTCAATGAATCTGATTTCGTGAATTTATCTGTCTTCGATAGAGAAATGGTAGA ACCTGATAGGGTTTTCTTTGTACGTGTTATGCGTGAGTGGATCTCAAGATTTACTGGTAAAATTATTGAAAATTTTGAGA TTCATCTCTCCCAGCCGATGGGTTTCGCAGGAGATATAATGTCTCTCACTGAATTTGCCACCTCCCGACAAGTCAAGAAC TTAGTTCTTGATTTCTCAGATCCTATCCAAAGAAACTTAAGTCAAGCCCAACAGATTATAAGAGATGGTCTACTCAGATG CAGATACCCTCAAAAGAACGAATTAGATGTTTCTCATCTCTTTTTCAATCTTTTATATGTTAGAAATTTAACGGTCTGCT CTTTTTTCCTTCAGGTCCTCCAAGATTGTGACGATCCTATGGCTTTGCACGTTTCGATGAAAACTCGACATTTGGTGATG AAAACAAATATGCACGCAAATGACTTCGTGGGAATTAGCATATTTCTCAATAGTTGTCCGGAACTGGAATCACTCACGTT TGATTTGGTTACTAGTAGTCGTTTCGTGAGAGCTCCATCGCCTTTAGTGATAGATCCTGTAAGCCATTGGCTCACTAGCA AATCTTACGAGTGTTTGGAAAAAACGCTTAAGGTTGTGAAGATAACAAAATTTCGTGGAAGCTCGAACGAGTTACATATG CTTCAATACTTGCTTCGTACCGGGTGTGTTATGGAACGAGTTGATCTATACGAGGCAGAGGGATTGAACCATAACCAAAA GAGGTGGGTACTAGCTGGGGTTGAGGAGGTTCAGCAGAATTTCAAGAGAGCTTCGAGGCATTTACGAATTACTCTACACA ATGCTTGATTCAAGCATTTTCTAGTGAAAAAGTAATCAATGAATGATCTTTTTGAACTTTGCTTTATTTCATATGAAGGA TGCGTATGTTTTATTTGGATTGGTTGT ...................................N...............N............................ ................................................................................ ..................................N.......................................N..... .......................N........................................................ ...............................N........................................i....... ............................N................................................... .................i....................i......................................... ................................................................N............N.. ..........................N.............................N....................... ......................................i.................N....................i.. .........N.........N............................................................ ................................................................................ .............................................................................N.. ..............................N................................................. ................................................................................ N.....................................N...N.............................N......N .....N..................... Pos Score Pred ------------------------ 36 0.171 - 52 0.161 - 195 0.451 - 235 0.363 - 264 0.447 - 352 0.152 - 393 0.567 Yes 429 0.346 - 498 0.685 Yes 519 0.703 Yes 625 0.224 - 638 0.206 - 667 0.191 - 697 0.152 - 759 0.842 Yes 777 0.367 - 798 0.516 Yes 810 0.352 - 820 0.341 - 1038 0.467 - 1071 0.391 - 1201 0.200 - 1239 0.088 - 1243 0.064 - 1273 0.084 - 1280 0.038 - 1286 0.050 -

Explain the output -- Processing locus 773: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGGTACTTGTTATAGTATTTGATACTCTTATTTACTTTCTGTTGGTGACATGCATAATGCTGATTTCACTGTCTCTTAT ATTTTTATGATCCAAACATGAAACACATACATAATCCATCCTCAACCAATGGAAGAGTCTATCAATCAAAATCCTGAAGC TATGGAAGCACTTATCTCCAATCTCTTTGGAAACATCTCGTCTTTGAAATCTGCTTATATCGAGCTTCAAAGTGCTCATA CTCCTTACGATCCCGAGAAGATTCAGGCAGCGGACAAAGTTGTCATTTCTGAACTCAAGAATCTTTCCGAAATGAAGCAT TTTTACAGAGAGAATAACCCCAAGCCTGTATGTGTCTCTCCACAAGACTCTCGTTTAGCTGCAGAGATTCAAGAGCAGCA GAGTTTGTTGAAGACTTATGAGGTCATGGTGAAGAAGTTTCAGTCTGAGATTCAGAACAAGGATTCCGAGATCACGCAGA TGCTGCAGAAGATTGAGGAAGCAAACCAGAAACGGCTTAAGCTTGAGAAGAATCTTAAGTTAAGAGGAATGTCTACAAAC CAAGGTTCTGGTGGAGATGGAAATTTACAGTTTCCTGACTTGACTACTGAACTCTTTGTATCTACTTACGAAGCTGCTGC TAAAGTTGTGCATGATTTCTCCAAGCCGCTAATCAACATGATGAAAGCAGCAGGATGGGATCTTGATACTGCAGCCAATT CTATTGAGCCTGATGTTGTTTACGCCAAGAGGCCTCACAAGGAATATGCATTTGAATCATACATATGCCAAAGGATGTTC AGTGGGTTTCAGCAGAAGAACTTCTCAGTAAACTCAGAGAGTGCTACGGTTATGGCCGATGATGACACAGACACCTTTTT CCGCCAGTTTCTTGCTCTCAAGGACATGGATCCACTAGATGCTCTAGGTACAAACCCTGATTCCAACTTTGGTATATTCT GCAGGAGCAAGTAGCTCCTCTTAGTCCACCCAAAGATGGAAGCTTCTTTCTTTGGAAATCTAGACCAGCGTGACTACGTG ACAGGAGGTGGCCACCCGAGGACTGCATTTTAC ..................................................i......N...................... ......N..........i..............................i............................... .......................................................................N........ .............................i.................................................. .................N.......N.....................................................N ....................................................................i........... ................N............................................................... ...........N.........................i..i.............N......................... ............N................................N..................i.........i..... ...................................................i......N..N.................. .........................N............N......................................... ...................................N............................................ ................................. Pos Score Pred ------------------------ 51 0.566 Yes 58 0.314 - 87 0.212 - 98 0.517 Yes 129 0.501 Yes 162 0.598 Yes 312 0.456 - 350 0.725 Yes 418 0.209 - 426 0.375 - 480 0.252 - 549 0.632 Yes 577 0.257 - 652 0.143 - 678 0.744 Yes 681 0.880 Yes 695 0.408 - 733 0.169 - 766 0.134 - 785 0.848 Yes 795 0.713 Yes 852 0.542 Yes 859 0.208 - 862 0.214 - 906 0.461 - 919 0.425 - 996 0.172 -

Explain the output -- Processing locus 774: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTAATTCTTATCACGTTGGATTTATATAGATGTAAACTTCGTAGTAATAACAATGATGAGTCGTGTTTTAAAAGAATTT TGTTACTTTCTTGTGTGATAAGCTTCCTCGCAATTTCCTACTGAGGAAAAGAAATGCAATTTTAACTTCCTTCAAAAGTT TATAGTAAAAAAAACAAAACTCAACATCTCATTGCTTGCATATATGTATAAATACCTCCCACTTCTAGTAACAAAGATCA CACCGAGAAAGGCAATGGAGTCGGCTAAATCAATCATCGGTGGCCACCAAAATGTCATCCTGATGCGTCATGGTGATCGA CTCGACAATTTCGAGCCACTCTGGACTTCAACAGCTGCGAGACCGTGGGATCCACCGCTTGCTCAAGACGGTAAGGATCG GGCCTTTAGAACTGGTCAGAGAATCCGGTCTCAACTTGGGGTTCCGATTCACCGTGTCTTCGTCTCCCCTTTCCTCCGCT GCATCCAGACCGCTTCCGAAGTCGTTGCCGCCCTCTCAGCCGTCGATTTCGATCCCATTGCTATGTCATCTAAAGACGTG CTTTCCATCGACAATACTAAGATCAAGGTGGCTATTGAATTTGGATTGTCCGAGATACCGCACCCAATCTTTATTAAGAG TGAAGTTGCTCCCAAAGATGGGAAATTTGATTTCAAGATTTCAGATCTTGAAGCTATGTTTCCTGAAGGAACAGTGGATT CTAATGTGGATATGGTTTATAAAGAGGTGCCAGAATGGGGAGAATCTGCACAAGCCTTTGAGGATCGATATTATAAGACA GTGAAGATTCTTGCGGAGAAGTATCCTTCTGAGAACTTGCTCTTAGTCACTCATTGGGGAGCAGTAAGTGTTGCATTTTA CAACTACTTCAAAGACGCAACTAAGTACGTGGTAGATTATTGTGGTAGTGTTGAAATGAGAAGGCAGATTTTGAATGGTG ATGGGTTTGGTAAATTTGAGGTAGTTACTAGTCATGGAGTATCTTATAAGTACACCAAAATCCCAGTCCATGATCATGTA GTTGTAAGCCGCCAATCTCCCCTTTAACCGGTTTATATGTGAATATCTGGTTTAACGGGTTGCTTCAATAAAAAAAAATA TATAAGAATAATGGTTCAGTTATTTTCCTTTTCTTCCACAAATTTT ..............................N......................i..N....................... .....................................................N.......................... ...........................................N.................................... ..............i....................................i..........i......N.......... ................................................................................ ................................................................................ ..............................................................i................. ................................................................................ .................N.....................................i........................ ...N.......i......................i............................................. ................................................................................ .......................................................i..................N..... N................................N...................................N.....i.... ....................................N........................................... ..........N................................... Pos Score Pred ------------------------ 31 0.258 - 54 0.505 Yes 57 0.391 - 134 0.458 - 204 0.387 - 255 0.636 Yes 292 0.616 Yes 303 0.628 Yes 310 0.385 - 543 0.680 Yes 658 0.311 - 696 0.557 Yes 724 0.088 - 732 0.560 Yes 755 0.501 Yes 936 0.596 Yes 955 0.240 - 961 0.236 - 994 0.272 - 1030 0.170 - 1036 0.514 Yes 1077 0.108 - 1131 0.104 -

Explain the output -- Processing locus 775: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATAAACAAGAATCATGCAAAGAAATTTTGTTCTTTATGCTTATGGAGTTTATAGTTAGTCAAATTATTAACGGTAAGCT CTTTGTGTTGGTTTTGTTTGAATGTCAGAGATGTATAATAATATGGGACCCCGGCCTGGAATGGCAATGCCTCAGGCAAA TCCTCAGCCTAGCCCATTTGGGAATCCGTTCTCTGGACCTGGCTCTGGACTTATCCGGAGTGGCTTGGGAGCTTATGGGG AGAAGATTTTCGGTTCTAGCTCTGAGTATGTGCAGAGCAACATAAGTCGCTACTTCTCCGATCCTCAATACTATTTTCAA GTGAATGACCAGTATGTGAGGAACAAGTTGAAGATTGTTCTACTTCCATTCCTTCACAGGGGACACTGGACTCGAATATC AGAACCAGTTGGGGGTAGACTTTCATACAAACCACCGATATACGATATAAATGCACCAGACTTATACATTCCGCTTATGG CCTTTGGCACTTACGTTGTTCTTGCTGGTTTGTCACTAGGACTCTATGGAAAGTTTACTCCAGAAGCTCTGAACTGGCTT TTTGTGAAAGGAATGGTTGGCTGGTTTTTGCAAGTGATGCTGCTGAAGATAACATTGCTTTCACTTGGAAGCGGAGAAGC CCCTCTCTTGGATATTGTGGCATATGCAGGCTATACTTTCACAGGCCTTTGTCTAGCTGTTCTTGGCAAGATAATCTGGG GATACTCTTACTATGTTTTGATTCCGTGGACCTGCTTATGCACTGGGGTATTCTTAGTGAAGACAATGAAGCGAGTTCTC TTTGCAGAATCTAGGAGTTATGACTCAAGCAGGCATCACTATCTCTTGATCTTTGTTGCTTTAGCCCAGTTTCCTCTTTT GATCTGGCTTGGTAACATTAGTGTGAATTGGCTCTTTTGAAACGCATTACGTTTATATTGTTGTGAAAAACACCTCTGAG GTTATGTAGCAGAATTTGGAATGTCCTCCTGATGGATATATTATATGGTAGTTGAGCTT ..............i.....................N.....N..................................... .....................i........i...........i.................N.....i............. ..........................................................................N..... ...........................N.................................................... ....N........N.................................................................. ..................................................N.........................N... .............................................N.................................. ............N.......................N........................................... .......................N........................................................ ............N........................N...........................N.............. ...................N............................................................ ................................................................................ ...N................N..........N............N.............. Pos Score Pred ------------------------ 15 0.630 Yes 37 0.293 - 43 0.213 - 102 0.809 Yes 111 0.709 Yes 123 0.802 Yes 141 0.490 - 147 0.659 Yes 235 0.353 - 268 0.388 - 325 0.332 - 334 0.313 - 451 0.194 - 477 0.439 - 526 0.092 - 573 0.390 - 597 0.433 - 664 0.358 - 733 0.397 - 758 0.208 - 786 0.331 - 820 0.234 - 964 0.043 - 981 0.072 - 992 0.041 - 1005 0.043 -

Explain the output -- Processing locus 776: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACAGTAGAAAAGACAGAGAGATTAAAACAAAAGGTCAAATATAATTTCAATAAAAATAATAATTTAAAAGTGATCAAA TTTGTATTTAATTTTAGTTAAATCAAATCAGCCCCCACAAAAACATTAAAAAAAACTCCATTAAAGACCAATATAAGAGA GAACATGAGAGTCCAAACTTCATTCAATTCAAGTCTCAGTTCCAAGAATAAAAACAAAGATTTTGTATTTTCATTTAAAG AGATGAAATCAATCTTAGCCAACACTTCTCTCGACTCTTCATTCTCTCTTTCTAAACGTTACTTCAATTGGAAGAAGAAG AAAGTTCAAGAAGACGACGACGAAGAAGAGGAAGAAGAAGAAGACGATTACAACCACAACGAGAAAAAAGTCTTGACCCG TTTCAATTTCTCCTCTGACCCGACCCGACCGGATCAGTTCAATGCCCAACAGATAATGAAGAAAAAAAAGAAAAAGACTC TCGAAAAGATCCGTTACGCTCTCGGGTTCTCTAACTCGGGTCTGGGTTTCCGGGTCGTGGGTACGTTATTCGGTAACCGT CGTGGACACGTGTACTTCGCCGTACAAGATGACCCGACTCGTTTACCTGCGGTGTTGATCCAGTTACCGACTCCAACGAG CGTTCTTGTCCGTGAAATGGCTTCAGGGCTAGTGAGAATCGCGCTCGAAACGGCTGCGTATAAGACAGATTCAAAGAAGA AGCTTTTAGAAGAGTCCACGTGGAGAACGTATTGTAACGGTAAAAAATGTGGTTACGCGGCGAGGAAAGAGTGTGGAGAA GCGGAGTGGAAGGTGTTGAAGGCGGTTGGGCCTATAACAATGGGTGCTGGAGTTTTACCGGCGACTACGACGACGGTGGA TGAAGAAGGGAATGGAGCGGTTGGGTCCGAAAAAGGTGAACTCATGTATATGAGAGCCCGGTTCGAGCGGGTTGTCGGGT CGAGAGACTCGGAGGCGTTTTACATGATGAACCCTGATGTGTCAAGTGGTGGACCTGAGCTTAGTGTGTATTTTCTAAGG GTTTAATGCGAAAAAAAGATAAGATTAGTATATGATTAAGAAGGTATTATATGACCATTTTAGTTTTTAAGAATGTGTTT TGGTTTTTGGCTTTTGTTTAGATGA ................................................................................ ................................................................................ ....i........................................................................... ..i............................................................................. ................................................................................ .........................................N.............i........................ ................................................................................ ............................i................................................... ................i............................................................... ..............................................i................................. .......................................i.......................................N ...........N...............................N.....N.............................. .......................N..i.........N........................................... .....N.........................N..................N.....................N....... .....................N... Pos Score Pred ------------------------ 165 0.512 Yes 243 0.670 Yes 442 0.438 - 456 0.596 Yes 589 0.558 Yes 657 0.676 Yes 767 0.772 Yes 840 0.863 Yes 880 0.170 - 892 0.269 - 924 0.317 - 930 0.387 - 984 0.184 - 987 0.521 Yes 997 0.172 - 1046 0.139 - 1072 0.057 - 1091 0.060 - 1113 0.057 - 1142 0.030 -

Explain the output -- Processing locus 777: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTAATTTATATAACTCTTATTTTCTTTCTTTAGTTATTTTTCCCTTATTCTTTTTCCAGTTTATCTAAACAAAAGGTA ATTAATTTCAAAATTCGATAAACCCTACTATTTGACATCTTCTACGAACGGGCACACCGGAGAATCAGCTTCTCGTTCCG TACGATGTCGGCGACACTCCGACGCCTCATTCTTCTCACCTCCACAAATCGCTCCCGTAATCTATCTCCGCCGTTTCTTT CCACCGCCGTGAACCACCTATCTCTATCATTCTCTTCCGTCTCATCTTCACCGGAATCACACACTTCCCCTTCCCGGATC CGAACCCGAACACCACTCGAAACTCAATTCGAAACATGGATTCAGAATCTAAAACCAGGATTCACAAACTCCGACGTTGT GATAGCGTTACGAGCTCAATCCGATCCAGATCTAGCTCTCGACATATTCCGATGGACAGCTCAACAACGAGGCTACAAAC ACAATCACGAAGCTTACCACACTATGATCAAACAAGCAATCACCGGAAAACGAAACAACTTCGTCGAAACGTTAATCGAA GAAGTAATCGCCGGAGCTTGTGAAATGAGTGTTCCTCTCTACAATTGCATCATCAGATTCTGTTGTGGTCGTAAGTTTCT CTTCAATAGAGCTTTCGATGTTTATAACAAAATGCTTCGATCAGATGATTCAAAGCCTGACCTTGAAACTTATACTTTGC TACTAAGTTCATTGCTTAAGAGATTCAACAAATTGAATGTGTGTTATGTGTATCTACACGCCGTTAGATCGTTAACGAAG CAGATGAAATCGAATGGTGTGATTCCGGATACGTTTGTGTTGAATATGATTATCAAAGCTTATGCAAAGTGTCTTGAGGT TGATGAAGCTATAAGAGTGTTTAAAGAGATGGCTTTATATGGCTCTGAGCCTAATGCTTATACTTATAGTTACTTAGTTA AAGGGGTTTGTGAGAAAGGTAGAGTTGGACAGGGTTTAGGGTTTTATAAGGAGATGCAAGTCAAAGGAATGGTTCCGAAT GGGAGTTGTTATATGGTTTTGATTTGTAGTTTGTCTATGGAGAGGAGATTGGATGAAGCTGTTGAGGTTGTGTATGATAT GTTGGCGAATTCGTTGTCTCCGGATATGTTGACGTATAATACGGTGTTGACGGAGTTGTGTAGGGGAGGTAGAGGGAGTG AAGCTCTTGAGATGGTTGAGGAATGGAAGAAACGGGATCCGGTGATGGGTGAAAGGAATTACAGAACGTTGATGGATGAG GTTTATTTTTTGAATAAGGGATGATTGATTGGGTTCTGTTTATCTGAAAGGCGATCAATCGGCTAAGCTTAAGGGCTACA AGATGGGGGGGGTTTGTTGCAAAGTCTGTGTTCTGCACTCTAG ................................................................................ ................................................................................ ....i........................................................................... ................................................................................ ...................................i............................................ ...................................................N............................ .......................i........................................................ ........................N....................................................... .................N.............N............N................................... ....................................N........N.................................. ...i.........N...............................N...............N.................. ..N.........................i.........N..............N.......................... .....................................................N..............i.........N. ............i.......................N...............N....................N....i. .........................N...................................................... ...........i..........N.....................N..........................N...N.... ....................N........................................................... ..N........................................ Pos Score Pred ------------------------ 165 0.685 Yes 356 0.603 Yes 452 0.447 - 504 0.510 Yes 585 0.489 - 658 0.279 - 672 0.228 - 685 0.149 - 757 0.414 - 766 0.183 - 804 0.532 Yes 814 0.164 - 846 0.397 - 862 0.198 - 883 0.078 - 909 0.691 Yes 919 0.330 - 934 0.111 - 1014 0.315 - 1029 0.622 Yes 1039 0.076 - 1053 0.541 Yes 1077 0.425 - 1093 0.063 - 1114 0.093 - 1119 0.528 Yes 1146 0.494 - 1212 0.617 Yes 1223 0.222 - 1245 0.372 - 1272 0.306 - 1276 0.373 - 1301 0.194 - 1363 0.095 -

Explain the output -- Processing locus 778: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATAAAGAGACTCTGCATTCTCTTCTTTTCTTCATCAAATATTTTCTCTTCTTCGTCTCTCTCACGTCTATTATTTTCT GTCATCTTTTATTTGAGGTAATTCCTTCTTCTAAACCATAAAACCAACCATATTGTTCTACATATATTCTTCTTTTTAAG TTCTTATAAATATAAATGTTCTTTGTTTTTGTTTATGATTTTTAGAACCACCAGATTTGCAGTTTCTGGAGAAATGGAAT TTGTGAACAGCTCTCCACCAAGATATGAATGTCTCCTCTTTGATTTGGATGATACTCTTTATCCTTTAAGCTCTGGCTTA TCAGATGCATGCTCCAACAACATTATAGAATATATGGTTGAAAAGCTTGGAATAGATGAAGATGGAGTCGTTGAACTAAA CCAAATTCTTTATAAGAAATATGGAACTTCAATGGCCGGTCTTAAGGCGGTAGGTTATGAATTCGATAATGACGAGTACC ACCGGTATGTTCATGGTAGATTACCTTATGAAAACCTTAAACCGGACCCGGTTCTAAGGAGTCTCCTCCTTGGTCTACCT TTGCGAAAATTGGTATTCTCGAATGGAGATGAGGTTCATGTGATGAAGGCTCTCACAAGACTTGGCATCGAAGATTGTTT TGAGAGAATCATAAGTTTCGAGACCTTAAACCCTGATATCAACGAAGCTGAGCTTTCTTGCGTCACAGGTCATCTTCCCG AGAATCCGGTTATTTGTAAACCAACTGAGATTGCTTTCGAGAAAGCCTTCGACATTGCACAGCTCAATCCTCACAAAACC TTGTTCTTTGACGACAGTACCCGGAACATCCAAACCGGGAAAGCCGTTGGTCTCCATACAGTCTTGGTTGGTAAATCAGA GAAAATAGATGGAAGCGATTACGCGTTAGAAAGCATTCACAACATGAAAGAGGCATTCCCCGAGTTATGGTCGGAATCCA TTATCAACAACAAAGAAACTGAAAGAATTGATTACGCTTCACAGATCTCGATTGAAACTTCTGTTCAAGCTTAACTTTAT TTAGTACTAAATATGATGATATTTACATATCATTGAATGTTTCAATAAAATATCTATGTACCTTTTTTTGTCGTCTATCC ATGTACCGTTTAT ................................................................................ ................................................................................ ...............N..................N......................................i...... ........................N...N...................N............................... ....N...N........................i.....................N.....i.................. ....................N..........i........................N...........N........... ......N.....N..............N.................................................... ......................i.....N........N....N..................................... ................................................................................ ................................................................................ ................................................................................ ........N..................................N......................N............. ................................................................................ ............N..N....................N..................N........................ N............ Pos Score Pred ------------------------ 176 0.405 - 195 0.303 - 234 0.804 Yes 265 0.405 - 269 0.175 - 289 0.092 - 325 0.306 - 329 0.059 - 354 0.808 Yes 376 0.140 - 382 0.612 Yes 421 0.328 - 432 0.733 Yes 457 0.324 - 469 0.439 - 487 0.205 - 493 0.091 - 508 0.156 - 583 0.543 Yes 589 0.225 - 598 0.085 - 603 0.422 - 889 0.486 - 924 0.361 - 947 0.203 - 1053 0.100 - 1056 0.066 - 1077 0.108 - 1096 0.039 - 1121 0.066 -

Explain the output -- Processing locus 779: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGATCTTCCACTCCCACGCTGCATAACCACAATCTCTACATATCTTCTACACGTGAATCTAGTTTGTGGAAATCTAAAAC CAACCAATCTACACTCGCTGACGTGTCTATATGTTAATCTTCCCACAAAATGGAGTAATGAACACTTTACTATATAAACG GCGTCTCCAATTCAACAACATCTCATTCTCACTTACTTCTTTTCTTTTTTTGTGTTCTTGTTTAGAAGAAGCAATGGCCA CTTCAGCAATCCAACACTCTTCTTTCGCTGGCCAAACGACCCTAAAGCCATCCAACGATCTCCTCCGCAAAATCGGAGCC TCCAATGGCGGTGGCCGCATCATCATGCGTCGTACCGTCAAGTCTACTCCTCAGAGCATCTGGTACGGACCAGACCGTCC CAAATACCTAGGACCATTTTCCGAAAACACACCATCATACCTAACCGGAGAATACCCTGGAGACTACGGTTGGGACACCG CTGGTCTCTCAGCCGATCCAGAAACATTCGCAAAGAATCGTGAGCTCGAAGTGATCCACAGTAGATGGGCAATGTTGGGA GCTTTAGGCTGCACCTTCCCTGAAATTCTCTCAAAAAACGGAGTCAAATTCGGTGAAGCCGTGTGGTTCAAGGCAGGATC TCAAATCTTCTCAGAAGGAGGGCTTGACTACCTCGGAAACCCTAACTTGATCCACGCGCAAAGCATATTAGCTATATGGG CGTGTCAAGTTGTGCTAATGGGATTCATTGAAGGGTACAGAATCGGAGGTGGTCCTCTTGGGGAAGGGCTTGACCCGCTT TACCCGGGCGGGGCCTTCGACCCGTTGAACTTAGCGGAGGATCCAGAAGCGTTTTCAGAGTTGAAAGTGAAGGAGCTTAA AAACGGTCGTCTTGCTATGTTCTCAATGTTTGGATTCTTTGTCCAAGCCATAGTTACCGGTAAAGGTCCGATCGAAAATC TGTTCGATCACATTGCAGACCCTGTGGCTAACAATGCTTGGGCTTACGCCACCAACTTCGTCCCCGGAAAATAGAGTTTG ATCGGATAATTTATGTAAATTATATCTTTTAAACATTTATCAATGCATTAAAGTTATCCCTATATTTGATACATCGGTTC CATTTTTTTTTTT ................................................................................ ..............................N..................N.......N...................... .........................................................................i...... ................................................................................ ....i...................i....................................................... ................................................................................ ................................................................N......i........ ................................................................................ ...........................................................................i.... .................i.............................................................. ................................................................................ ................i........i...................................................... .................................N.............................................. ............N.............................N..................................... ............. Pos Score Pred ------------------------ 111 0.160 - 130 0.383 - 138 0.378 - 234 0.876 Yes 325 0.796 Yes 345 0.596 Yes 545 0.354 - 552 0.756 Yes 716 0.688 Yes 738 0.737 Yes 897 0.623 Yes 906 0.537 Yes 994 0.240 - 1053 0.056 - 1083 0.071 -

Explain the output -- Processing locus 780: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACTTTGGACTAATGATTTAATCAAAGATGTCATTTTAACATATATGTATAAAACGCGTAATTGGGAAGATACACACACA CACATACCAGCTATGACGTGACAGTGTGTGTTAGATATTGAGATGATGAGCTGGCATCAACCTCTCTATAGTCTCTATAT AATCCATTCTTTGTCCTCTCTTCTCTTTACATGTAAATTCACATATCTCTCTCTCTTATTTGATCATAAAGATATTATTG CTTCTTCAAGTAAACAAAAAATGGGAAGCTTTCTCTTCCTCATCGTAGTCATCTTCCTCTTCTCTTCATCCGTTAACGCT TGTGATCGATGTCTTCACCGTTCTAAAGCAGCTTATTTCTCCTCTGCCTCTGCTCTCTCTTCTGGAGCTTGTGCTTATGG CTCTATGGCTACGAGTTTCTTCGCCGGACATATCGCTGCAGCTATCCCTTCTATCTACAAAGACGGTGCTGGCTGTGGAG CTTGCTTTCAAGTCAGATGCAAGAACCCTAAGCTGTGTAGCACTAAAGGAACCATTGTGATGATCACAGACTTAAACAAG AGTAACCAAACCGATCTTGTCCTTAGTAGCAGAGCTTTTAGAGCTATGGCTAAACCTATTGTTGGTGCTGACAAAGACCT TCTCAAACAAGGCATTGTCGACATCGAATACCAAAGAGTTCCTTGCGATTACGGCAACAAGAACATGAACGTGAGAGTAG AAGAAGCAAGCAAGAAACCAAACTACTTAGAGATAAAGCTTTTATACCAAGGAGGTCAAACAGAAGTAGTATCCATCGAC ATTGCTCAAGTCGGTTCATCGCCAAATTGGGGTTACATGACAAGAAGCCACGGAGCTGTTTGGGTAACTGACAAAGTACC CACCGGAGCTATCCAGTTCAGGTTCGTAGTAACCGGTGGATACGACGGTAAAATGATTTGGTCACAGAGTGTTCTTCCAT CCAATTGGGAAGCTGGGAAAATTTACGACGCCGGTGTTCAAATCACTGACATTGCTCAAGAAGGTTGTGATCCTTGCGAT GCTCACATCTGGAACTAAGTTATTTAGTGGCACACAAACAAACATCTTTCATAGGTTTATATATAATGTGTGACAGAGGT TATTAATGAACATAGTTAAAGAATGCTTAATCTAATG ............N..............N................N................................... ............N.............................N..N.................................. ..............................N................................................. ....................i........................................................... ........N...................................................................N... ....i........................................................................... ................N..........................................i.................... .............................................i.................................. ................................................................N............... ................................................................................ ....................................i........................................... ....................................................i........................... ..............................................................................N. .................................................................N.............. .....N................N...........N.. Pos Score Pred ------------------------ 13 0.286 - 28 0.327 - 45 0.462 - 93 0.118 - 123 0.081 - 126 0.312 - 191 0.385 - 261 0.829 Yes 329 0.197 - 397 0.456 - 405 0.815 Yes 497 0.256 - 540 0.659 Yes 606 0.651 Yes 705 0.419 - 837 0.694 Yes 933 0.776 Yes 1039 0.189 - 1106 0.074 - 1126 0.039 - 1143 0.062 - 1155 0.041 -

Explain the output -- Processing locus 781: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAGAAATCTAACAGTCAATGGAACTCAGAGAACGACCAAAAACTAACAGTCAATATGCAAAGATTATCTGTGGGAAACT TACCATATATATTAGTGTTGAACCAAGATGCTGAGAGTAAGAAAGGCAATGGAGAATAAGAACCAGAAGCGGTTTGTGCT CATCCATGGGGTGTGCCACGGGGCATGGACTTGGGACAAGGTGAAAACGCAGCTGGAGGTTGCAGGTCACTGTGTGACGG CAGTGGATCTTGCTGCATCAGGTATAAACATGACCAAAGTGGAAGAGATTCAGACTCTGAACGATTACTGCAAACCATTG CTTGAGTTTCTGAGCTCGCTTGGCTCAGATGACGGTAAGGTGATTGTTGTTGCTCATAGCATGGGAGGAATATCCGCTGC ACTTGCTGCTGACAGCTTCGCTTGTAAGATTGCCGCTATTGTCTTTTTGACAGCTTTCATGCCCGACACAATAAACCCAC CTGCTTATGTTTACGAAAAGCTGCTCAGAAGCATTCCACAAGAGGAATGGTTGGACACCACGTGTGTGAACTACGGGAAA CCTGATTTTCCTCTACAGTATACTCTTTTGGGACCAAAGTTTATGGCCAAGAAAATGTATCAAAACTCTCCAGTTCAAGA TCTTGAAGTGGTGAAGACATTAGTGAGGGAAAACCCGTTAGTTACAAACAATCTGGCAGGGACAAGAAGCTTTAGTGAGG AAGGGTACGGATCCGTTACACGTATATATATTGTATGCAGAGAGGATCTTGTGGAAGTCGAAGATTACCAGCGTTGGATG ATTAGCAACTTTCCACCAAAAGAAGTAATGGAGATCAAATGTGCAGATCATATGCCAATGTTCTCCAAGCCTCAAGAAGT TTGTGCTCTTCTCCTGGAGATTGCAAATAAATATTGCAAAAATTAACCAATTTCAGCTTCCACCAATAAATAATACGCCA TAAGAGAATACATCTATATGTATGAGAACTAAGCAAATGATAATGCTTATATAATAAAGTTTCGT ..................i....................................i........................ ...........................N....................i............................... .....N..................N....................................................... .............................i.................................................. ............................N...............................i................... ..........................................................i..................... ......N.......................................i................................. ..........................................i...........N......................... ................................................................................ ..................................N..........................................N.. ...........................i..........N............N.....N...................... ................................................................................ .................N...N..............N.....N...................... Pos Score Pred ------------------------ 19 0.663 Yes 56 0.605 Yes 108 0.423 - 129 0.565 Yes 166 0.243 - 185 0.474 - 270 0.604 Yes 349 0.154 - 381 0.600 Yes 459 0.631 Yes 487 0.189 - 527 0.736 Yes 603 0.692 Yes 615 0.455 - 755 0.391 - 798 0.166 - 828 0.593 Yes 839 0.221 - 852 0.340 - 858 0.479 - 978 0.148 - 982 0.050 - 997 0.047 - 1003 0.055 -

Explain the output -- Processing locus 782: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCCGGAATCAACTATCTTCGATCAGAGCAAGTGGGGATAAGAGATATGACATGAGCTTGCAAGTCTGGTTTGCGATGAGA CAAGGAGCCACAAATTCTTGATTTTGTTCTAGTTTGTTTCAAAATTATTCAAAAAACGTTGCACTCGATGTAAAAACGTT ATTTTCTTTTGAATCTAATTTAACATTATTTTCAAAAAAAAAATATATATATTCGTTTTTAGAAGAGAATATTTGGCCAC TGTGTAATTTATCTTGTATTTTGATTAGATTTATGTTCAAGAAAAAAAAACAAAATTTGACATATCTATTGATTTTTTTT TCTTTTTGTTCTCTTATCCTCTTCGCGCTTCCACACGTCGTCAAATTACCAATGACGTCTCTTCCAATAATCCTCGCATC CTCTCCACTGCGAAACCTAACCAAACCTTGTTCAACTTCTCAAATCCCAAAACCCATTCAAAATTCTACAAAACAACCTC CAATACATCTCCTAACCAAAACCAATCTCTCTGTAACCATTTCCCAACTAATTATCACTTCCCCTGTTCTCGCATCTGAA TCATTCGACTCAATTTCATCAGACCCATCTTCTGGAAAAATCGATTTAGAGTCAATTTTGGTCACAATTGATAATTTCTT CAACAAGTATCCATTTTTCGTAGCGGGATGTACTTTTACCTATCTTGTTGTTTATCCAGCTGTGATGTTTTACTTGAGGA AGTATAAACCAATATCCGCCATGAACGCGTTTCGTAAGCTTAAGAATGAATCTGATTCTCAGCTTTTGGATATTAGAGAT GTCAAGACTTTGGCTTTGTTAGCATCACCGAATCTTAAGTTTTTGGGTAAGAGTTCTGTTCAGGTTCCGTTTAGTGAGAA TGATGAAGAAGGGTTTTTGACGAAAGTTAAAGGAAGCTTCTCTGATGCTGAGAATACAGTTGTTTGTGTTCTTGATAATT TTGATGGTAACTCGTCGAAAGTAGCTGAATTGCTGATCAAGAATGGTTTCAAAGAGGCTTATTATATTAGAGGCGGCGCG AGAGGGAAGAATGGTTGGTTGGCTATTCAAGAAGAGCTTTTGCCTCCACCGGTGCATATGTACACTGCGAAAAACGTAAA ATCTTCAAACAACAATGAAGCGTCTGTTGTTGGAACTGAGAACTAGTTCAAGAACATAGCGTAATGGTATGCAGATTCTT GTTGTCGGTATATAGTTTCTCTTTTACTTTTTCTCTCTAATAGCTTTTGCTTCGGTTTTGCAGTG .............................................N....N.......................N..... ...................................................................N............ ................................................................................ ................................N............................................... ...................................................i............................ ................................................................................ ................................................................................ ................................................................................ ...........................N....................................N............... ....................N........................N................................N. ...............................................................................N ..N.........................................N................................... ...N......................................N..................................... ..........N..............................................N...................... ..............N................................................N....N........... ................................................................. Pos Score Pred ------------------------ 46 0.438 - 51 0.431 - 75 0.157 - 148 0.202 - 273 0.112 - 372 0.640 Yes 668 0.189 - 705 0.453 - 741 0.265 - 766 0.237 - 799 0.179 - 880 0.098 - 883 0.096 - 925 0.214 - 964 0.146 - 1003 0.410 - 1051 0.257 - 1098 0.409 - 1135 0.259 - 1184 0.167 - 1189 0.074 -

Explain the output -- Processing locus 783: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATCGAGATTGATACTCCTCCTAGTAATATATATATTACTCCTGTCACACTTCCGAGGAGCTTGTTTACATGGTGCAAAA TGCTTGTGACATTAAAACTAAGGAACGTGGTTCTTACCGATGATGTTACTTCACTACTGTTTTTCCCATGCATCCCTCAA AAACTTGAGTCTTATATCCATGAAGTACCCAGGTGGTGATGAATTTTTCAACACGCTTATATCAAGTTGTCCAATTCTTG AAGACTTGGTTGTGAAACAAATCCTCAACGACAATTTGGCCATTGTGACTGTTAGAGTGCCTTCTCTAAAGAGGTTAGTC TTGAACCAAGAAGTAAAAAACGTTGTTAAAAGCATAGCTAATGGGTTTGTGATAAACACTCCTGCTTTGGAGTGCATGAA TATTGTTGATTTTTCGACCGGGTGTAACATTGTTGAGAGTGATATGCCTAACATTGTGAAGGCAAAAGTTGACATTTTTC ATCCACGAACTGAGCAACTTTTGGGTTCTACAACTTTAGCCAAGTATCTTCATCTCTGCTTACCAACCTCAAAGAATGTG TCGTATCCTGCTGGTAATATCTTCAACCGTCTCGTACATCTAAGGCTATGCACATGTGAGACTGAGTGGATTAATCTACT TATGCGTTTACTCAGAAATTCCCCTAGTTTACGAGTTCTTGAGATTGAACAGGTCCATTACCTTCGATATTACCAACCAC GACCGTGCTGGAGTGAACCAAGCTCGATACCTGAATGTCTTACTCATAATCTTGAAACCTTCAAATGGGAGCATTATTAT GGAGCAGAAGAAGAGAAAGAAGTGGCGGCATTCATCTTAAGAAGTTCAATCTGTTTAAAGAAGGCAACTATCATCCTTCA TAGGTCTATTGTCCACGACGAGAAACTTGAGATGAACAAGGAATTGTCATTATTGCCAATATGTTCACCAATTTGTCAGC TTGCTTTCAGCTGAGGTTATGGTCAGTTTTAGATCAGCTATACTACAACTTATTATTTCTTGAATTTTCATAATTAGCTC TTTGGAATTTCTATCTTTCCACAAACAGTTTGC .....................................................................i.........N .......................................N..N........................N............ ...................i..................N......................................... ................................................................................ ........................................N..................................N.... ...........................................N.................................... ...........................................................................i.... ...............................................N.....N.......................... ..................................N.............................i.............N. ................................................................................ ...............................N............................N................... ..................N............................................................. ................................. Pos Score Pred ------------------------ 70 0.666 Yes 80 0.412 - 120 0.374 - 123 0.225 - 148 0.344 - 180 0.525 Yes 199 0.169 - 361 0.286 - 396 0.385 - 444 0.333 - 556 0.567 Yes 608 0.265 - 614 0.453 - 642 0.363 - 755 0.175 - 785 0.637 Yes 799 0.476 - 912 0.459 - 941 0.150 - 979 0.137 -

Explain the output -- Processing locus 784: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTTTCCGATCACATCCTTGAGAAGAGGAAAATTTCGAAGTTATTTGATTCATTCTTCCGACTGAGTTTCCCGCCATTAT TTTGTTTGATCGGAAAATTAAATGGAAAGAGTGAATCTCGGAGGTTTAGGTTACGATAATGGCGGAGTTATGATGACTAG AGATCCCAAACCTAGGCTCAGATGGACAGCCGATCTTCATGATCGCTTCGTCGACGCCGTCGCTAAGCTCGGTGGCGCTG ACAAAGCAACTCCTAAATCGGTTCTGAAGCTGATGGGATTAAAAGGTTTAACACTGTACCATCTCAAGAGCCATTTACAG AAGTATAGACTTGGTCAACAACAAGGCAAAAAACAAAATAGAACAGAACAAAACAAAGAAAATGCTGGAAGTTCATATGT GCATTTCGACAATTGTTCTCAAGGAGGAATCTCCAATGATTCAAGATTTGATAACCATCAAAGACAAAGCGGGAATGTAC CTTTTGCTGAGGCAATGAGACATCAGGTCGATGCGCAACAACGGTTTCAAGAACAACTCGAGGTGCAGAAAAAACTGCAG ATGAGAATGGAGGCACAAGGAAAATATTTGTTGACATTACTAGAGAAAGCACAGAAGAGCTTACCTTGTGGTAATGCAGG GGAAACAGACAAGGGTCAATTCTCGGATTTCAATCTCGCACTTTCGGGACTTGTAGGAAGTGATCGTAAGAACGAGAAGG CGGGTTTGGTTACTGATATCTCACACCTTAATGGTGGTGATTCCTCTCAAGAGTTTCGGTTATGTGGAGAACAAGAGAAA ATAGAAACCGGAGATGCATGTGTTAAACCAGAATCCGGGTTTGTGCATTTTGATTTGAACTCCAAAAGTGGGTATGATCT CTTGAATTGTGGGAAATATGGGATTGAAGTGAAGCCAAATGTGATTGGAGATAGACTTCAATAGGTTAAAGAGACACTTT CTTTAATTTTTTATTTTATTTTATTATTTCTTTACATTTCTTATAGAATGTTACAAATTTGGCTTCATGGTTTCTCAGTC AAA ................................................................................ .....................i....................................i..........i..i....... .....................N................N......................................... ................................i............................................... .............................................................i..............N... ...................................N......................................i..... ..............N...............N................................................. i.....i..................................................................N...... ................................................................................ ..............................N..............................N.................. .............N...N.......................................................N...... .................N....................N......................................... ...............................................N..................N............. ... Pos Score Pred ------------------------ 102 0.545 Yes 139 0.802 Yes 150 0.821 Yes 153 0.859 Yes 182 0.383 - 199 0.295 - 273 0.517 Yes 382 0.668 Yes 397 0.341 - 436 0.343 - 475 0.551 Yes 495 0.248 - 511 0.388 - 561 0.538 Yes 567 0.757 Yes 634 0.483 - 751 0.143 - 782 0.159 - 814 0.469 - 818 0.169 - 874 0.056 - 898 0.089 - 919 0.157 - 1008 0.044 - 1027 0.055 -

Explain the output -- Processing locus 785: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATCTTTGAAAAGTCAACGTTTGGTCGCAATCGGTGACGTACACTGTCTATATTTAATTTTTCAGCAACCACAGTGATTT ATTGAATGAATCAATACATAACAACAAAATTATCAGAAACACAATTCCTTCTTCTTCTTCTTCTTCTTCTTGTCTCCGAT GTCAGCCATGGATGTTATGATTCACTCTTCAAGCTTTCTCCTCCCTTGCGACGAAACTAGTACAGGGACGAGATACGCTC TCGTTGTTCTGAACCAGAGTTTGCCACGATTCACTCCTCTTCTCTGGGAACATGCAAAACTTCGTCTCTGTGCTGATGGA GGCGCTAATCGCATCTACGACGAATTGCCTCTCTTCTTCCCTAATGAAGACGCTTTGGCCATTCGAAACAGGTATAAGCC GGATGTTATCAAAGGAGATATGGATTCTATACGTCGTGACGTCCTCGACTTTTATATAAACTTGGGAACTAAGGTTATAG ATGAATCTCATGATCAAGACACCACAGATCTTGATAAATGCATTTTGTATATCCGTCACTCTACTTTGAATCAAGAGACT TCCGGACTCCAGATTCTTGCCACTGGAGCACTTGGAGGAAGATTTGATCATGAAGCCGGTAATCTCAACGTCTTATATCG ATATCCAGATACAAGGATAGTCCTTTTATCTGACGATTGCCTCATCCAACTCCTTCCAAAGACTCATCGACATGAAATAC ATATTCAGTCTTCTCTAGAAGGGCCTCACTGTGGACTCATACCCATTGGAACTCCATCTGCTAAAACCACAACCTCAGGG CTTCAATGGGATCTCAGCAACACTGAGATGAGATTTGGTGGGTTGATAAGTACATCCAACTTGGTTAAAGAAGAGAAAAT CACAGTCGAATCGGATTCGGATCTTCTCTGGACTATATCCATCAAGAAAACAGGACTTTCCATACAAGACCATACACCTT AGGCCCGGTAACAAAACACACTTTAGTATATTATACGCTACTATGATGTTCCTGATGCAACGAACTAGTTCAACATTATT GTGTTGATTTGTTGTTCACTG ................................................................................ .....N........................................................................i. .......i...N....N............................................................... ...................................................N.......................i.... ...........................................N.................................... ..N................i............................................................ N........N...........................i.......................................... .................................................i.............................. .......................................................................i........ ................................................................................ .....N.....................i.................................................... ................................................................................ ..........................................N..N........N......................... ..................... Pos Score Pred ------------------------ 86 0.297 - 159 0.711 Yes 168 0.694 Yes 172 0.421 - 177 0.280 - 292 0.498 - 316 0.572 Yes 364 0.167 - 403 0.258 - 420 0.754 Yes 481 0.205 - 490 0.182 - 518 0.526 Yes 610 0.602 Yes 712 0.512 Yes 806 0.298 - 828 0.685 Yes 1003 0.056 - 1006 0.049 - 1015 0.035 -

Explain the output -- Processing locus 786: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTTCGTGCTGTGAAAAATGGGAAAAGTTTTATGATTTTACAAGTTAGAACAGCAGACTGCTCACGAGGTAATACCTTTA TTCTCCTCTTTCTTTAGGTAGGTCTTAGCTCGACGTCAAGAACTCACTAAAATCATGCTGCTCACTTCTTGTTTCGCTGT ATACGTACACTACTCCTCCACCATTACCTCCTCAGCTAGTACATTATCTCAATCTATAACAACTTTATATTACAATATTC CTCACATATACAAGCTTTGGTTATTCGTTTGCTCTTGTTTTTATCAGGTAAGCATCATTTGTAGACTACATTTTCAGAGA GAAATGGGTATATTCCCTGGCTTGATCAATCAGAACACACAACAGCCTCCAGAGGTCGAGTCCAAGAGATCTAGGAATGT TAAAACTAAGTCAGTGTCTGAGACTTATCCAGAGAACAGAGAAGAATTAAAGAAGCAATTAAAACTTTGGAGAGCTTCAG ATAAGAAATCTCCATGGTATGATTATCCTCCTAAAGTTAAGGTGACAAACGAAAAGGATCTTTACCATTTGAACATGAAA TTTACAATAGGATTACCTCCTGAAGCAGTCTTCGATATACTCACTACTTATGAGAACCCATCATACTTCACAATGATGAA AAAGCGCCAAACTCTGGAACACGTATCAAGTAAAGTTTTTAGCGATCTTGGACCGACAGAGAAGCACGTGAGGGTGGAAA AAGCTGCTCCCTGGAGGTTCCTTTGGTGGTCTGGATCTATCCCGGTACATCTAACTTTCAATGAGAGCCGGAAAGATTTC AGCACACTATATATGATTCCTAAAAAGAACGTGATGTTCATGAAAACGTTCTATGGTAAGTGGCAAATAGAGCCATGGTA CGTAGATAACATGCGCTTTTGCAAGCCGAGGCTCCCAAAGAATCGAGAAGAATATAGACAATGTACCGGTGGAAAAGGAT TGATTGGCTCGAGAGTGACATTGGACCAGTATTTTCAGCCTTCTTCTTATCTTAACCTGCCACCACTTTCTTGGTACATC CGTAGGGCCACCGTCAAGACCACCAAGGCTCTAATCGAAGATCTTCAAATTCAGGCCGCCGTTATCAGAAGCGTTTGAAA CTCTTTCCAATCCGATATTTGCTAGTTATACCATTTTTATACAAACATTAACCTCAAATATTGGAATACTGTAAAAACAA TTTCACGTTCTGTCAAG .................i.............N................................................ ......................................................N......................... ................................................................................ ................................................................................ ...i........................................................................N... ................................................................................ .............N....N.......................................................N..... .................................................N......................i..i.... ................................................................................ ............................................................N................... ............N....................N.....N............i.....................N..... ..........N.................................................i................... ................................................................................ ................................................................................ ................................................................................ ................. Pos Score Pred ------------------------ 18 0.505 Yes 32 0.303 - 135 0.469 - 324 0.696 Yes 397 0.421 - 494 0.183 - 499 0.095 - 555 0.355 - 610 0.253 - 633 0.767 Yes 636 0.643 Yes 781 0.222 - 813 0.368 - 834 0.297 - 840 0.252 - 853 0.583 Yes 875 0.446 - 891 0.288 - 941 0.667 Yes

Explain the output -- Processing locus 787: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATATGGTAATTTTTTCTTTTTCTGGCTGATTACATAATGATAATGTTAATGATCAGGATGCATATTGACTAAATAAACT TGAATACAATTACTAATATAGTATGTTTCTACGTTATATATATGCCCACCCATATGGCTCCCATCAAAATTCATCATTAA TCAAAGCTTAGTAATAAAATATCATTTAAGAGGGATAATAATCATGAAACTCTTAGAAAAAATGATATATGTAGAGTTTT TGATGATAATAATGGTGATATGGGTCGTGCCCATGAGTTACGGTCATGGAGCGATGATTGGTAATGCAGTCGAAGCACCT GATGTAGCGGAAGCACCAGGGATTAATGACCCAAGCAAGGCACTCGACACTAATTGGTATGACGCACGAGCCACGTTCTA CGGTGACATCCATGGTGGCGACACTCAACAGGGAGCTTGTGGATACGGTAATCTATTCAGACAAGGCTATGGTCTAGCCA CCGCAGCGTTAAGTACGGCACTCTTCAACGACGGGTACACATGTGGGGCTTGTTATGAAATCATGTGCACGCGTGATCCA CAATGGTGTTTACCCGGATCCGTCAAGATCACAGCAACAAACTTCTGTCCCGCAAATTACAGCAAAACCACAGACCTTTG GTGCAACCCACCACAGAAACACTTTGATCTCTCCTTAGCAATGTTTCTCAAGATTGCCAAGTACAAAGCCGGGGTTGTCC CAGTTAGATACAGACGTATTCCTTGTAGCAAAACTGGTGGTGTCAAGTTTGAAACCAAAGGAAACCCTTATTTCTTAATG GTCTTGATATACAACGTAGGAGGAGCTGGAGATATTAAGTACGTGCAAGTTAAAGGAAACAAGACCGGGTGGATAACAAT GAAAAAGAACTGGGGGCAGAATTGGACCACTATTACAGTATTGACCGGCCAAGGTTTATCGTTCAGGGTTACAACGAGTG ATGGGATTACAAAAGATTTTTGGAATGTGATGCCAAAAAATTGGGGGTTTGGACAGACTTTTGATGGTAGGATTAACTTT TAGGAGAATGATTTGAGTTTTATTATATGTGTCTTACACATAAGTTATGTAATACGTACTTGCAATAATTAAAAATAAAG AAGATACTATTTATCCATAAAA ...i.................................N.....N.....N........N..................... ......................N..................N...........i.......................... ...........................................N.................i......N........... ..N........i.......i............N............N.......i.........i................ ...........i........................................................N........... ........................................i.............N.......N................. ..i............................................................................. ........................................i....................................... .............................................................................i.. ..............................................................................N. ................................................................................ N.......................N....N.................................N................ .......N..................N...................N................................. ...................... Pos Score Pred ------------------------ 4 0.510 Yes 38 0.386 - 44 0.320 - 50 0.255 - 59 0.338 - 103 0.306 - 122 0.328 - 134 0.583 Yes 204 0.367 - 222 0.578 Yes 229 0.378 - 243 0.337 - 252 0.855 Yes 260 0.571 Yes 273 0.435 - 286 0.308 - 294 0.649 Yes 304 0.501 Yes 322 0.457 - 346 0.341 - 379 0.560 Yes 412 0.659 Yes 469 0.331 - 521 0.517 Yes 535 0.438 - 543 0.479 - 563 0.599 Yes 681 0.633 Yes 798 0.545 Yes 879 0.441 - 961 0.144 - 985 0.220 - 990 0.157 - 1024 0.279 - 1048 0.161 - 1067 0.098 - 1087 0.085 -

Explain the output -- Processing locus 788: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCAACTCACCTCTTATTATTTATAAGTAATGCCCATTACTTGATCCAACGCTCTCTTGTTTATATTTTTGACTAACTCAA GCTCTCTCGGTTTTGCTTTAATTCAGCATTAGTTATATGTGTGATTTATTAGCTTGCCTTAGAAAAATTTCAATGGGGAG AAAACCGTGCTGTGACAAAATTGGATTGAAGAGAGGTCCATGGACTATAGAAGAAGATCATAGACTCATGAACTTCATTC TTAACAATGGCATCCACTGCTGGAGAATTGTCCCCAAACTCGCAGGTTTGTTGAGATGCGGGAAGAGCTGTAGATTGAGA TGGATCAATTATTTGAGACCCGATCTCAAGAGAGGTGGTTTCACTGATGCTGAAGAAGATCGGATTATGGAACTTCACTC TCAACTTGGCAACCGGTGGTCTAAAATTGCCTCGCATTTCTCTGGTCGAACGGATAACGAGATCAAAAACCACTGGAATA CGAAGATCAAGAAGAAGATGAAACATCTTGGGTTGGATCCGGCTACGCACAAACCGATGAATGATATTACTCATCAAACC GATCCTAATCAAGATAAAAAACCAAATATGTGTTCTACCATCAATGAAGGAGAAGAGATCAAGGATCAAACTCCAAAAGA TGATGTTATCACAGAAACAACAAAAACATTAATGTTATCTGATAATGATGAGGAACTTGTGGCGAAAAACTGCAAAATTC TATGTGCAGAAGAAGTAGACTTGGAATCTCTATTTGAAACGCAATGCAATGAGATATCCTCTTCGTCTTTCTCTTCACTA TGTAGTAATATTTCAAGAAGTGAATCTTCATCATATCTTGCAGAAGATTCGATCTCTTTAGAGCAATGGGACTTGGATAT GACGGATCCTTTTGTACCATGGGACCTCTTCGCCAATCTTGATGATAATCTTTTCCTCTTATGATGAATGTACTATAACA TTGTAATATAATATATCTATATAATTATCGCAAGTAATTAGCAGTTTCTTTTATGTAAAATGTGGATTATGCATGGCGTG TGA ............................N................................................... ....................................N...................................i....... .......................................N...........................N............ ......i................................................N.......................N ..............................................i...................N............. ................................................................................ .................i......................................i...N................... ...........................i...............N...................................N ..N............................i............N..N................................ .................................................................N............i. ..................N......................N..................N..N...N............ ....................................................N......N........N...N....... ... Pos Score Pred ------------------------ 29 0.398 - 117 0.299 - 153 0.628 Yes 200 0.412 - 228 0.163 - 247 0.707 Yes 296 0.314 - 320 0.351 - 367 0.524 Yes 387 0.325 - 498 0.694 Yes 537 0.529 Yes 541 0.171 - 588 0.755 Yes 604 0.272 - 640 0.134 - 643 0.064 - 672 0.687 Yes 685 0.282 - 688 0.146 - 722 0.404 - 764 0.136 - 769 0.529 Yes 800 0.232 - 866 0.273 - 879 0.501 Yes 899 0.112 - 922 0.138 - 941 0.246 - 944 0.276 - 948 0.192 - 1013 0.056 - 1020 0.059 - 1029 0.065 - 1033 0.085 -

Explain the output -- Processing locus 789: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGATGTTGTTTAAAGGTGAGTCCAAATTCCACGCGCCGCCTTGACTTTCATGAATGTGTGTTTTGATACATTTCCTACAT ATTCCGATGTTTTCTAGCTAGATCTCCAACGATTTTCTGATTGAATTTAGGGTTGGTTACATGATGATCTTTGACTCTAA CTCGCAGGTTTTAGCTAATAAGGGCATGAAACGGATCTGCTCGGTTTCAAGTGTTCAACTTTTTTCCAGAAGTTTCAGAG CTTTAGCATCGCCACGTTCCTTGAACTATCCCCTGCAGTGTATCAAGCGTTCATCAGTTCGGATGGAGAGTAGCAACTTT TCTTCTGGTGTCAGAACCGAGAGTAGTGTGAAGCCAAGAGGAGCATTGATAGTTCTAGAAGGTCTTGATCGCAGCGGGAA ATCAACACAGTGTGCTAAGCTACTCTCTTTCTTGAATGGTTTAGGACATCCGACCGAGCTCTGGAGATTCCCCGATAGAG AGACGAGTGTCGGTCAGATGATATCCGCGTATCTTTCTAATAAGTCGCAGTTAGATGATCACACAATTCATCTCCTTTTC AGCGCCAATCGTTGGGAGAAAAGGTCGTTGATGGAGGAAAAGCTAAAAACAGGAACTACGCTTATCGTGGATCGTTATTC GTATTCTGGTGTTGCATTCTCATCAGCTAAAGGTCTCGATATCGAATGGTGCAAGGCTCCCGAAATCGGGTTACTGGCTC CAGATTCTGTACTTTACCTGGACATTTCACCTGAAAGAGCTGCTGAGAGAGGTGGATATGGAGATGAGAGGTATGAGCGA GTGGAGTTTCAGAAGAAAGTTGCAGACTTTTATCAAACCCTTGGCGATTCTTCATGGAAGATAATCAATGCCAGTGATGC CATGGAGGAAGTAGAGAAGAAGATTCAACAAGTGGTATTAGATCAAGTCAAAGAATGTACTGAAGGAAAGCCTCTGTCTC TCCTATGGTCGTCATAGAACGTTTTCGAGTCATAGCACTTGCAAGTTTAGTGATTTTGAAGGAACTTTTGTTGAAGTTTA TACTATGTTGTGCCATCTAAGCCCATTAACAGGCCC ..N..............................................N...N.......................... ......N.....................................................N..N................ .........................N...................................................... ..............................................................i................. ................................................................................ ...................................N............................................ .................i....................................N......................... ..............................i................................................. .............................................N.................................. .........................................................N.....N........N....... .....................................................N.............N........N... ....N........................................................................... ....N............................... Pos Score Pred ------------------------ 3 0.227 - 50 0.204 - 54 0.368 - 87 0.470 - 141 0.344 - 144 0.443 - 186 0.281 - 303 0.726 Yes 436 0.252 - 498 0.725 Yes 535 0.192 - 591 0.698 Yes 686 0.389 - 778 0.311 - 784 0.145 - 793 0.375 - 854 0.308 - 868 0.330 - 877 0.152 - 882 0.588 Yes 935 0.583 Yes 965 0.136 - 1045 0.079 -

Explain the output -- Processing locus 790: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATAAGTGTAAAGTTTCTTATCCGCAGAGCCAAAATGTCGCTTTTAAAAAGAAAAATTCCAAAGTTATTTCTTTATGCA AGAAAAAATTCGAAATTTTAATTATCACGTAAAATCATAAAAGAATCTCAAAGGAAAGCGACAATTTTGGAAAATTCAAC GATAAGCTTTATAATTTTTCTACTTATGAGTGGATGTGTGAAGAGTAGGGAAAAAGTACGAAAGGAAAATATGAGTGAGG AGAAGAGGAAGCAACACTTCGTGCTAGTACATGGTGCGTGCCACGGCGCATGGTGCTGGTACAAGGTTAAGCCTCTTCTC GAGGCTTTGGGCCATCGTGTAACCGCCTTAGACCTAGCTGCTTCCGGTATAGACACAACCAGGTCAATCACTGACATTTC TACATGTGAACAATATTCTGAGCCATTGATGCAGCTAATGACTTCATTGCCGAATGATGAGAAGGTTGTACTCGTTGGTC ATAGCTTTGGAGGTTTGAGTTTAGCCTTAGCCATGGATAAGTTTCCCGATAAAATCTCTGTCTCTGTCTTCGTGACTGCA TTCATGCCCGACACCAAACACTCACCATCGTTCGTCGAGGAAAAGTTTGCAAGCAGCATGACACCAGAAGGATGGATGGG CTCTGAGCTCGAGACATATGGTTCAGATAATTCCGGCTTGTCTGTGTTCTTCAGCACCGACTTCATGAAGCACCGTCTCT ACCAACTTTCTCCTGTGGAGGATCTTGAGCTTGGATTGCTTCTAAAGAGGCCTAGTTCATTGTTTATTAATGAATTATCG AAGATGGAGAACTTTTCTGAGAAAGGGTATGGATCTGTTCCTCGAGCTTACATTGTGTGCAAAGAGGACAACATTATCTC GGAAGACCATCAACGATGGATGATCCATAATTATCCGGCGAATTTAGTGATTGAGATGGAAGAGACTGATCATATGCCAA TGTTTTGCAAACCTCAACTACTAAGTGACCATCTATTGGCAATCGCTGACAATTTCTGTTAAATAATATTTTGATGAAAA TGTATTTGGAGTGGATACAATCAAACGTGTTCTAAAATGGATCTTCATAAAGGTTCAAACTTATTTCTTAGTTCTTACTA ...................................N.......................................N.... ................................................................................ .........................N.......N....................................i......... ..............................N..................N.............................. ................................................................................ ...N........................i........i...............N..N....................... ................................i............................................... ...i.....................................................i.............i...N.... .................N..............................................N............... .....................................................................N.......... ...N........................N................................................... ...............N...N...................................i.................N.....N .........................................................................N.....N ....................................N........................................... Pos Score Pred ------------------------ 36 0.369 - 76 0.194 - 186 0.338 - 194 0.435 - 231 0.579 Yes 271 0.170 - 290 0.389 - 404 0.425 - 429 0.660 Yes 438 0.553 Yes 454 0.124 - 457 0.203 - 513 0.730 Yes 564 0.657 Yes 618 0.650 Yes 632 0.696 Yes 636 0.469 - 658 0.310 - 705 0.172 - 790 0.471 - 804 0.407 - 829 0.326 - 896 0.341 - 900 0.229 - 936 0.511 Yes 954 0.209 - 960 0.251 - 1034 0.086 - 1040 0.056 - 1077 0.136 -

Explain the output -- Processing locus 791: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACCGGCGCAATTTGAACGAATTAATTCAAAATCGATATCATACCAAATCAAGTTCGGTTTAACTACTATACCAGATTAG GTTATAACCTTGTTAAAATAGGCGGCAATGGAAATTTATTGGCGCCATAAATTTTCTGAGAAGCAAAATTTTAACTCCAT TACTTATCTCTGGTGTGTACTTTCTCGGCGAAGGAGTTACACGAGAACGTTGAAAATGGAGAATGATGGAAGATCCAATG AGAAAAATCTGTACGAGGTTCTTGGTGTTGAAGCAACAGCATCTCCACAAGAGATAAGGAAAGCGTATCATAAGTTAGCA TTGAGGCTTCATCCTGATAAAAACAAGGATGATGAGGATGCTAAAGAGAAATTTCAGCAGTTGCAAAAGGTTATTTCGAT TCTCGGTGATGAAGAGAAAAGAGCAGTCTATGATCAAACTGGCTCAGTTGATGATGCTGATCTTTCGGGAGATGTGGTTG ACAACTTGAGGGACTTCTTCAAGGCAATGTACAAGAAGGTCACCGAGGAAGATATTGAAGAGTTTGAGGCCAACTATAGG GGTTCTGAATCCGAGAAGAATGATTTGATTGAGCTATATAACAAGTTTAAGGGTAAAATGAGCAGGCTTTTCTGCTCAAT GCTTTGCTCGAACCCCAAGCTTGATTCGCACCGTTTCAAAGATATTATCGATGAGGCCATTGCAGCGGGAGAAGTGAAAT CAACAAAAGCTTACAAGAAATGGGCAAAGGAGATTGCAGAAATGGAACCTCCCACGAATCCCCAGAAGATGAGACGGAAA GCAGCAAAGGCTGCAGATAAAGATTTATACGCTGTAATGTCCCAGCGGGGAGATGAGAGGAAAGAGAAGTTCGATTCAAT GTTCTCTTCACTTGTCTCTAGGTATGGTAGCAATGCTGACTCTGAGCCAAACGAAGAAGAGTTCGAAGCTGCTCAGAGAA AAGTTGAAAGCCGGAGATCATCGAAGAAGTCTAGAAAAAAATAAAGATTATGTAGTAGTTTGTGAAATTTAGGTTTCGTG TTTGTAATGGAGACCAATGTTTTTAGCGTAAATTAGATCACATTTGTTGCTTCTTAGAAGTTG ................................................................................ ...........................N.................................................... .......................................................i......N..i...........N.. ................................................................................ ............................N..N.....N.......................................... ........N....................N....................N..N.................N........ ..........................N..................................................... ...................N.....................................i....................i. ..................................................N............................. ...................i.....................i..........................N........... ....................................i...............N.........................N. .......................i........N............................................... .................................................N.............................. ......N.........N.............................................. Pos Score Pred ------------------------ 108 0.206 - 216 0.872 Yes 223 0.319 - 226 0.647 Yes 238 0.178 - 349 0.305 - 352 0.331 - 358 0.093 - 409 0.155 - 430 0.161 - 451 0.075 - 454 0.100 - 472 0.242 - 507 0.258 - 580 0.338 - 618 0.691 Yes 639 0.762 Yes 691 0.128 - 740 0.709 Yes 762 0.811 Yes 789 0.292 - 837 0.653 Yes 853 0.486 - 879 0.420 - 904 0.502 Yes 913 0.400 - 1010 0.119 - 1047 0.090 - 1057 0.065 -

Explain the output -- Processing locus 792: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCAGTGCATATATAAGAACATAAGACATTAAGACAGAGTAGCACAGAGCTTCCATAACAAGACAAAGCCAAAGAGCAAA AGAGATCGGTAGATCTTATAGAGAGAACAAATTTTTCCTCAAATCAAAATCAGAAGAAGAATGAGTTGGTGGGGAGTGAA AGGAGGAGGATGGGGAATGGTGGAAGAAGTTTGGAGAAAGGGTCCATGGACCGCCGAAGAAGACCGTCTTTTGATCGAAT ACGTCCGTGTTCACGGTGAAGGTCGTTGGAACTCTGTCTCTAAACTCGCAGGATTGAAAAGGAATGGCAAAAGCTGCAGA CTAAGATGGGTGAATTACCTTAGACCAGACCTCAAGAGAGGACAGATCACTCCACATGAAGAAAGTATAATACTTGAGCT ACACGCTAAGTGGGGAAATAGGTGGTCAACAATTGCACGTAGTTTACCAGGAAGAACAGACAATGAGATCAAGAACTATT GGAGAACCCATTTCAAGAAAAAGGCAAAGCCTACGACTAACAATGCGGAGAAGATAAAGAGTCGTCTCCTAAAAAGGCAA CACTTCAAGGAACAGAGAGAAATAGAGTTGCAACAAGAACAGCAGTTGTTTCAGTTCGACCAACTCGGTATGAAAAAGAT CATCTCTTTACTCGAAGAAAACAATAGCAGTAGCAGTAGCGATGGCGGTGGTGATGTGTTCTATTATCCTGATCAAATAA CACATTCATCAAAACCCTTTGGCTATAACTCTAATTCATTAGAGGAGCAGTTACAAGGTAGATTTTCTCCTGTAAACATA CCTGATGCTAATACTATGAACGAAGACAATGCCATATGGGACGGGTTTTGGAACATGGATGTTGTAAATGGACATGGTGG GAACTTGGGTGTTGTGGCTGCTACTGCTGCTTGTGGCCCAAGGAAGCCCTATTTCCATAACTTGGTGATTCCATTTTGTT AAGTATGGTCTACTTTTGGACTTCGGTTTTCTGTCTTTTCAGGTTTGGTTACTAGTTTCTATGCTATGAACCTATAAGTC GTTTGTGATGAAGTGTGTGTG ................................................................................ ............................................................N................... .........N......i............................N.................................. ...............................................................i................ .....N.................................................i........................ ..............................................................N................. ..........................................i..................................... .....................................................................N.......... .........................................i...........N.......................... ................................................................................ ....N..........i............N......N..................i...N........N.....N...... ................................................................................ ....N.......................................................N....N.............. .......N............. Pos Score Pred ------------------------ 141 0.421 - 170 0.377 - 177 0.795 Yes 206 0.186 - 304 0.563 Yes 326 0.412 - 376 0.580 Yes 463 0.321 - 523 0.511 Yes 630 0.355 - 682 0.737 Yes 694 0.208 - 805 0.126 - 816 0.843 Yes 829 0.438 - 836 0.209 - 855 0.782 Yes 859 0.089 - 868 0.354 - 874 0.378 - 965 0.214 - 1021 0.046 - 1026 0.045 - 1048 0.044 -

Explain the output -- Processing locus 793: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCAAGAAGTCCACCAACGTGGTGGATTTTAAAATAACTAACCAAAAACACAAATCAGGGAAAAGTCGTAAATTAAAACTG ATAATTTGGAGGAAAATATAGAAAAAGAAAAAGGTAACGACATTAAAGAAGGTGCACTTAATGCGCAGTATGTCCTCTTG TGCCAGCTTGTTGCACCGTCTTCCGTCTCCTCCTCTTTCCCTCTCTCTCTCTCTCCAAACCAGTCCTACTTCTCTCTCTA GAAACCTAGGGAAGAAGAAGAAGACGGTGAAGCGAGCGATGTCTTCGGACGACGAAGGAAGAGAGGAGTATCTCTTCAAG ATTGTTGTTATCGGCGACTCTGCCGTCGGTAAATCTAATCTTCTATCTCGTTACGCACGTAACGAGTTCAGCGCTAACTC CAAGGCGACGATCGGAGTCGAGTTTCAGACGCAGAGCATGGAGATTGAAGGTAAAGAGGTCAAGGCTCAGATTTGGGACA CTGCTGGTCAGGAGCGTTTCCGTGCCGTCACTTCCGCTTATTACCGTGGCGCTGTCGGTGCCCTCGTCGTTTACGACATT ACCCGCCGCACCACTTTCGAAAGCGTTGGACGTTGGCTCGATGAGCTTAAGATCCATTCGGATACGACGGTGGCGAGAAT GCTGGTGGGGAACAAGTGTGATCTAGAAAACATAAGAGCAGTGAGCGTGGAGGAAGGAAAGGCTCTAGCCGAAGAGGAAG GACTCTTCTTTGTGGAAACATCGGCTCTTGATTCGACTAATGTTAAAACAGCTTTCGAGATGGTGATACTTGATATCTAC AATAACGTGAGCCGTAAGCAACTCAACTCAGATACTTATAAAGACGAACTCACCGTGAATCGGGTAAGTCTTGTTAAGGA TGATAACTCTGCCTCCAAACAAAGCTCTGGTTTCTCTTGTTGTTCTTCCACTTGATGATATACCACACCAAAATAATCCT TTTTTATTTCTCACAACTGTAACTTTATTATATAAATTTTTGTTTTTTTCTTTGACTCATGGAAGAAAAGAAAT ................................................................................ ............................................................N........N.......... ................................................................................ ......................................i......................................... ................................................................................ .....................................i.......................................... ................................................................................ ........................................N.....................................i. ................................................................................ .......................................N...................i.................... ...............................................................................N ......................................................N......................... ..........................................................N............... Pos Score Pred ------------------------ 141 0.261 - 150 0.341 - 279 0.786 Yes 438 0.827 Yes 601 0.226 - 639 0.523 Yes 760 0.142 - 780 0.560 Yes 880 0.308 - 935 0.077 - 1019 0.043 -

Explain the output -- Processing locus 794: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAACAGATACAAACACAAGTTTATTATTTACCAACTCTTATCTCAAAAGATTCTTCTCAATAATTTATCCTCCTTTGACC CCTATATAGACACACATTTGACACCATATGTCATGCACAAAACATCATCATCTCTTGATCTATCTTTCAGTTCTCTTGTC AATTTTCCCCCAAAAAGAAAAAGGATATAAGAAGATAATGGCATCTAGAGAAATGCTGATCATAATCTCGGTCCTCGCTA CTACACTCATTGGTTTAACAGTAGCTACTGACCATACCATTGGTGGTCCTAGTGGTTGGACTGTAGGAGCTAGTCTTCGA ACTTGGGCTGCAGGACAAACATTTGCTGTTGGAGACAATCTTGTTTTCTCCTACCCTGCTGCGTTCCACGACGTCGTTGA AGTCACAAAACCCGAATTCGATAGCTGTCAAGCGGTTAAACCGCTTATAACGTTTGCTAATGGAAACTCCTTGGTTCCTC TTACTACTCCTGGAAAAAGGTACTTCATTTGTGGAATGCCGGGACATTGTAGCCAAGGGATGAAACTTGAAGTAAACGTT GTTCCAACCGCAACCGTAGCACCAACCGCACCGCTTCCAAACACCGTCCCATCTCTAAACGCGCCTTCACCTTCTTCTGT TCTACCTATTCAACCTCTTTTACCTCTTAACCCTGTCCCAGTTCTCTCTCCATCTTCTTCTACTCCACTTCCTTCCTCCT CTCTGCCTCTCATTCCTCCACTTTCTCCGGCACTATCTCCGGCTACTGCAGCGGGGACTTCTCTGCCGTTATTCCCAGGC TCACCAGGTAGCTCTAGCAGCACAACATCCACCAAAACCGTCGGGACCTTCCCTTCTAGTACTACCGGTACAACGGCCGA TCTTGCTGGCGCAGACTCTCCTCCGGCTGACTCCTCCTCTGCCGCGAAAACACTTGTTTTAGGATTTGGATTCATGGTTG CTATGATGCTTCATTTGTTCTAAGTGTTCTTTTTTTAAGTGGAGAGAAATTTGTTTGTAGAGGGAAATGTTGTTTGTGTT TACTTGGGTGCTTTCTTGTGAAATTTACCACTATCTCCGTTT ................................................................................ ...........................N....N............................................... .....................................i..............i........................... ................................................................................ ................................................................................ ...........................................................N.................... ...................................i.......................i.................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ .........................................................................N...... ..N..N............................................................N............. .......................................... Pos Score Pred ------------------------ 108 0.327 - 113 0.467 - 198 0.862 Yes 213 0.884 Yes 460 0.292 - 516 0.678 Yes 540 0.726 Yes 954 0.365 - 963 0.242 - 966 0.356 - 1027 0.065 -

Explain the output -- Processing locus 795: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACAAGGATGAACATGATAATGTGAGAACCAGTATTGATATAATGTTTGCAAATATTAGAGAGTTCCGATTTCAATTTTT GTTTTCAGGAAGCAGTTTTAAATTATTCCCATGCATGTTGTTTGTGTAGGAGAAGAGAGTTTTCTAGTTAACTCTAGGTT ATTTTTATGTCTTAAGAGGAATGAATTACATCTCTGGATGCAGGTTTTGAACAGCTTGGCAGAAGAAGTGAAACTTGAGA GAGTAATGGAGAACTTGGAAGATCTGAAAATTCTGGCTGAGCAGTGGTCGCATCAAGGAATTGAATATCTTCAGAAGATA CCACCGAATCAGCTGTATGCCGCCATTGGTGTTTTGTTGTTTACAACCATCTTGCTCTTCCTATCCATTCGCCTGGTCAG ACGTACCAAATCCAACACTGTACTCCTTTCTGGGCTGACCGGAAGTGGAAAGACTGTGCTCTTTTACCAACTCCGAGATG GATCGTCGCATCAGGGCACTGTAACATCAATGGAACCAAATGAAGGCACTTTTGTTCTTCACAGTGAAAATACTAAGAAA GGAAAAATCAAGCCTGTTCATCTTGTTGATGTTCCTGGGCACTCTCGGCTTCGACCCAAGCTAGAAGAATTCTTGCCCCA AGCAGCTGCCATTGTGTTCGTAGTGGATGCCTTGGAGTTCCTCCCAAACTGTCGTGCAGCTTCAGAGTACCTATACGAGA TTCTGACCAATGCGAATGTTGTCAAGAAGAAGATTCCAGTCCTCCTTTGCTGTAACAAGACCGATAAACTCACTGCACAC ACCAAGGAGTTTATCCGGAAGCAAATGGAGAAAGAAATTGAGAAACTGAGGGCTTCAAGGAGTGCAGTCTCTACTGCTGA TATAGCGAATGACTTCACCATTGGAATTGAGGGAGAAGTGTTCTCCTTCACGCATTGCTCCAACAAAGTCACTGTCGCTG AGGCATCTGGACTTACCGGAGAAACCATCCAGATTGAAGACTTCATTCGAGAATACATCAAGCCATGAGCTCCAACATGC CTTCATAGATTGAATCTACCACAAGTTTCTTATGCTTTGGGCTGTGACTTTGATTTGAGTTTAGAGATCCTTTTGTATAT GAGATCT .......N.....N.....N......................N..................................... ..............................N...N............................................. ......i.............N................i.......................................... .....N.......................................................................... ................N............................................................... .............................................................................N.. .............................i.........N........................................ ............................N................................................... ..........................N..................................................... .........N.....i................................................................ ........................i....................................................... ........N....................................................................... ................................................................N...........N... ...............................N..............................................N. ....... Pos Score Pred ------------------------ 8 0.216 - 14 0.287 - 20 0.262 - 43 0.233 - 111 0.290 - 115 0.303 - 167 0.776 Yes 181 0.143 - 198 0.564 Yes 246 0.419 - 337 0.149 - 478 0.294 - 510 0.567 Yes 520 0.240 - 589 0.103 - 667 0.146 - 730 0.423 - 736 0.727 Yes 825 0.706 Yes 889 0.456 - 1025 0.197 - 1037 0.106 - 1072 0.091 - 1119 0.039 -

Explain the output -- Processing locus 796: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 797: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACTTATAGGCATCAATTCTATTCAATTCCTCTTCTTCGTTTTATGATTGATGGTTTGATCTCCAATTTGATTGTATATT GTCTGATCTTAATAGTGGTAAATTGTTATGTTCTCAAGGTGTAAAAAAAATCAGAAAATGGACAACTTGGAAGATTTGAA GATTGTGGCAGAGCAGTGGTCTAAACAAGGACTTGAATTTGTTCAAAAAATACCACCACCTCAGCTTTATACTGCCATTG GTGTTCTCTTATTAGCTACCATTTGGCTCTTATCTATTCGTTTGTTTAGACGTACAAAATCCAATACTGTACTCCTCTCT GGGCTAAGTGGAAGTGGCAAAACAGTTCTCTTCTATCAACTACGAGATGGATCTTCCCATCAGGGTGCTGTAACGTCAAT GGAACCGAACGAAGGCACCTTTGTGCTTCACAATGAAAACAACACTAAGAAAGGAAAAGTAAAGCCTGTTCATCTTATTG ATGTTCCTGGACACTCTCGGCTTATATCTAAGCTAGAAGAATACTTGCCTCGAGCCGCCGCTGTTGTGTTTGTAGTAGAT GCATTGGAGTTCCTCCCAAATATCCGGGCAGCTTCAGAGTACCTTTACGACATTCTGACCAATGCTAGCGTTATTAAGAA TAAGACCCCGGTTCTGCTTTGCTGTAACAAGACAGATAAAGTAACTGCACACACCAAAGAGTTCATCCGCAAGCAGATGG AGAAAGAAATAGATAAACTTAGGGTTTCGAGGAGCGCAATATCAACAGCTGATATAGCGAATGACTTCACTCTTGGGATT GAAGGAGAAGTGTTTTCCTTTTCGCATTGCCATAACAAAGTCACGGTCGCTGAGGCGTCTGGACTAACAGGAGAAACAGA TCAGGTACAAGAGTTCATCAGAGAACACGTAAAGCCATGAGGATTGTGTAAGCATCTCAGAGAATGTACTTAAATGTCTT TTCATTTGTTTGTAAATGAAATTATAATTTGAGAACTTAGATGATATTCGTTGGAAGGA ...........................................N......N............................. ...........................N.............................i...................... ................................................................................ ................................................................................ ..............................................N...............................i. ................................N............................................... N.............................................................................N. .............................................................i.................. ............................................................................i... ............................................................N................... ................................................................................ ....................................N..........................N.........N...... ...............N........................N.................. Pos Score Pred ------------------------ 44 0.243 - 51 0.395 - 108 0.412 - 138 0.621 Yes 367 0.388 - 399 0.632 Yes 433 0.254 - 481 0.080 - 559 0.094 - 622 0.545 Yes 717 0.687 Yes 781 0.400 - 917 0.286 - 944 0.087 - 954 0.107 - 976 0.035 - 1001 0.046 -

Explain the output -- Processing locus 798: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTCTCTCTTCATTAGTTCATATAAAACTTCTTATTCTTGGCTTTAGACAAATTCAACACTAATCACGTCTTGATTTTA TTACCAACTTTAAGCATTTGAAAAAAAAAACCAGTGGATTTCGTGTGGGCCCTGCATAAGCAGCGCGTGCAATCAATTTG TTTTTTTTCTCTACGCGTTTTCGCCACCTTATTTATTTTCATGCGTTCATATATTTTTCTCATTAATTCACATTTAAACA CTTCTCTGCATATATTTGCTTCTCCGTTTCTCATTTCTCAACTCTCAAACTCAAATCACTTCCTTTGTCTCAGTGACTCT AGTCTATCTCCAATGGCTGAACGAGTCTATCCCGCCGATTCACCACCACAAAGCGGACAATTCTCCGGCAACTTCAGCTC CGGCGAATTCCCCAAAAAACCAGCACCACCACCATCAACATACGTAATCCAAGTCCCAAAAGATCAAATCTACCGTATAC CACCGCCGGAAAACGCACACCGCTTTGAACAACTCTCTCGTAAAAAAACCAACCGCAGTAACTGTAGATGCTGCTTCTGC TCATTCCTCGCCGCGGTTTTCATCTTAATCGTCCTCGCCGGAATATCCTTCGCGGTTCTCTACCTAATCTACCGTCCCGA AGCTCCTAAATACTCCATCGAAGGATTCTCTGTTTCCGGTATCAATCTGAATTCAACATCTCCGATTTCGCCGAGTTTTA ACGTTACCGTTAGATCACGTAACGGTAACGGTAAAATCGGGGTTTACTACGAGAAAGAAAGCTCTGTTGATGTGTATTAC AACGACGTTGATATAAGTAACGGTGTTATGCCGGTGTTTTATCAGCCGGCGAAGAATGTAACGGTCGTTAAGTTGGTTTT AAGTGGTTCTAAAATACAGTTGACTAGTGGTATGAGGAAGGAGATGCGTAATGAGGTGAGTAAGAAAACGGTGCCGTTTA AGTTGAAGATTAAAGCTCCGGTGAAGATTAAGTTTGGTTCTGTTAAGACGTGGACTATGATTGTTAATGTTGACTGTGAT GTAACGGTGGATAAATTGACGGCGCCGTCGAGGATTGTGTCGAGAAAATGCAGTCATGACGTGGATCTTTGGTGATGAAT TTGCTCTTTGTTTTTTTTTTTTAAAGAAGAAAATAAGAAAAGAAATGAGTACAGAGGATGTTTTTGGATTTGTAGGATTT TTTTTTTATAAATT ................................................................................ ................................................................................ ........................................N....................................... ................................................................................ ............i................................................................... ................................................................................ ...................................................................N............ ................................................................................ ................................................................................ .....................................................................N.......... ...........................i...........................N........................ ...............................N...........N......N............................. ........................................................N.........N...........N. ...............................................N.......N..................N..... ............................................N............N...................... .............. Pos Score Pred ------------------------ 201 0.399 - 333 0.836 Yes 548 0.226 - 790 0.131 - 828 0.564 Yes 856 0.167 - 912 0.439 - 924 0.492 - 931 0.090 - 1017 0.248 - 1027 0.124 - 1039 0.114 - 1088 0.188 - 1096 0.284 - 1115 0.089 - 1165 0.104 - 1178 0.044 -

Explain the output -- Processing locus 799: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 800: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGATTGCCCCGCCGAGGCATTACTACGCCAAGTGCGATTAAGGTCCCATACAGTGTAACGGGACCCACTATAAGACAGC GACCGACCAATTGCGTGTTAGGAGAGTTTCACCAACCCCGGACCGGTTTTTACCGGATATAACAGAACCGGTACGAACCG GTCTCATTATCTTCCATCTTCTTTATATAGACCTCATGCCATGTGTGTGACTCACCAAGAAAAACACAATCGTTTAATCT CACCCAAGAAGACAAAAACACAGAGAGAGAAAGAGAGAGAAATGGCGACGACAACAACAGAAGCAACGAAGACATCATCG ACCAATGGAGAAGATCAGAAGCAGTCTCAGAATCTTCGACATCAAGAAGTTGGTCACAAGAGTCTCTTACAGAGCGATGA TCTCTACCAGTATATACTGGAGACAAGTGTGTATCCTAGAGAACCAGAATCAATGAAGGAACTCAGGGAAGTGACAGCAA AACATCCATGGAACATAATGACCACATCAGCTGATGAAGGACAGTTCTTAAACATGCTTATCAAGCTCGTTAACGCCAAG AACACAATGGAGATCGGAGTTTACACTGGCTACTCTCTTCTCGCCACCGCTCTTGCTCTCCCTGAAGACGGCAAAATTCT GGCTATGGATGTCAACAGAGAGAATTACGAATTGGGTTTACCGATCATTGAGAAAGCCGGCGTTGCTCACAAGATCGACT TCAGGGAAGGCCCTGCTCTTCCCGTTCTTGATGAAATCGTTGCTGACGAGAAGAACCATGGAACATATGACTTTATATTC GTTGATGCTGACAAAGACAACTACATCAACTACCACAAGCGTTTGATCGATCTTGTGAAAATTGGAGGAGTGATTGGCTA CGACAACACTCTGTGGAATGGTTCTGTCGTGGCTCCTCCTGATGCACCAATGAGGAAGTACGTTCGTTACTACAGAGACT TTGTTCTTGAGCTTAACAAGGCTCTTGCTGCTGACCCTCGGATCGAGATCTGTATGCTCCCTGTTGGTGATGGAATCACT ATCTGCCGTCGGATCAGTTGATTTGACTCCTCCCTACTCTGAGTTTGTCCACAGTGGATTACTTTCCATCTTCTTATACC TTTCAATCGCATTTTCACCAACCACTAAAATGGACCTTTT ................................................................................ ................................................................................ ...................................N....i....................................... .........................................i...................................... ....i.......................................................................N... ....................................................i........................... .......i.........i...............N...................i.......................... ......i......................................................................... ....i...N....................................................................... ..............................N..........................N........N............. ....N........................................................................... .................N.......................N.......i.............................. .....................................................N...............N.......... ................................................................................ .............................N.......... Pos Score Pred ------------------------ 196 0.473 - 201 0.572 Yes 282 0.840 Yes 325 0.582 Yes 397 0.273 - 453 0.568 Yes 488 0.572 Yes 498 0.580 Yes 514 0.124 - 534 0.807 Yes 567 0.819 Yes 645 0.795 Yes 649 0.300 - 751 0.097 - 778 0.213 - 787 0.247 - 805 0.307 - 898 0.166 - 922 0.106 - 930 0.669 Yes 1014 0.218 - 1030 0.238 - 1150 0.072 -

Explain the output -- Processing locus 801: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATATTAGGACGTTACATGTTTGAATGAATTAAAAAATAGGAGAAGATCTTTCCCTATATAAATGGCTAGGTTTCTGCTC AGTTTTAATCTACCTTCATAAACACGAAAGTACCAAAAAATGGCAACTTTCATCAGATTCACTGCTCCTTTGTTTTGCTT CTTCTTCTTATTCTCTCTCTTTTCTCACCAAACCATGTCCCAACCTCAACACATGGCCACTTTCTGCGACGACAGATTAT CCGACAACTTCACACAAACCAGCTCATACAAAGCAAACCGTGAAACCCTACTTTCTTCTCTCCGTGACCGTTCCTCCCTT GGAACCTATTCCAATGCCACAATCGGTCTTAGCCCCGACACAGTCTATGGCATGTTTCTCTGCCGAGGAGACCTCACCAA AACATCATGTTCGGACTGTGTCAAAACCGCAACCCTAGAAATCACTAAAAATAATAACTGCACTTCCAGAAAAACTGCTC TAATATTCTACGAGGAGTGTATGGTTCGATACTCCAATGTTTCCTTCTTCACGCTCGTAGAACTAGACGGGCCTTACGTC GCTAAATACTCTCTCGCCACTTTTCCTACTTCTCTTTTTAGTTCTTTCCAACAAACGTTATCCAATAAGGTAGAGCAACT AATTATCCTCATAGCATCAAAGTCTTCTTTGTCTGCTTCTACGCCTTATTACGTGAAGGATATATCACGTGTAAATGAAT TAGAGGGCTCGTATACATTAGACACGGTGGTTCAGTGTAGTCCTGATCTTGATCCAGCAAATTGCGGCGTCTGTTTGAGA CTTGTGGTCAAAGGTCTCTCAGGCTGTTGTACCAATGCTCGTTTTGCTCAGTTCTATCTTTCTAAATGTTTTTTGAAGTA TGACACAACCGGTTTGCCAACTTCTCAATCACCAAGCGGCTCATCGTCTATTAACGTTATGAAAGGTGAGCATAATATAT GCCCTCGACTCTAATCTTTTTTTTTTTACAAGTTACGTCAAAAGATAATGTTTTAATTTCTTCCCGCCAAGACACCGCAG CTGTTTGTGTAGTAATCAAACTTTATTTGTCTA ................i.......N.....................................N................. .......................................i........................................ ..................................i.................i........................... ................................................................................ .............i................................i....i............................ ......N......................................................................... ....................i...............N........................................... ................................................................................ ..........................................................................N..... ................................................................................ ..................................N..............................N.............N ..........................................................i...................i. ...............................................N................................ ................................. Pos Score Pred ------------------------ 17 0.539 Yes 25 0.363 - 63 0.397 - 120 0.675 Yes 195 0.755 Yes 213 0.508 Yes 334 0.690 Yes 367 0.523 Yes 372 0.601 Yes 407 0.289 - 501 0.620 Yes 517 0.385 - 715 0.481 - 835 0.394 - 866 0.357 - 880 0.175 - 939 0.549 Yes 959 0.774 Yes 1008 0.106 -

Explain the output -- Processing locus 802: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAAGATAGATACAACTACTATTTTAAGCATTACTATTATACTTTTTCACTCATTTTCATCCTAGTTAATTATTTTTTTA GCTAAGGTAAGGAAAACTTGATTTTTCTGGAAAGTGAATCATTTTATCATAAATAATTTGTTTGTGTCAATGACTTGCAC AGAACTGAGAAGGATGGGAAACGAATCATATGAAGACGCCATCGAAGCTCTCAAGAAGCTTCTCATTGAGAAGGATGATC TGAAGGATGTAGCTGCGGCCAAGGTGAAGAAGATCACGGCGGAGCTTCAGGCAGCCTCGTCATCGGACAGCAAATCTTTT GATCCCGTCGAACGAATTAAGGAAGGCTTCGTCACCTTCAAGAAGGAGAAATACGAGACCAATCCTGCTTTGTATGGTGA GCTCGCCAAAGGTCAAAGCCCAAAGTACATGGTGTTTGCTTGTTCGGACTCACGAGTGTGCCCATCACACGTACTAGACT TCCATCCTGGAGATGCCTTCGTGGTTCGTAATATCGCCAATATGGTTCCTCCTTTTGACAAGGTCAAATATGCAGGAGTT GGAGCCGCCATTGAATACGCTGTCTTGCACCTTAAGGTGGAAAACATTGTGGTGATAGGGCACAGTGCATGTGGTGGCAT CAAGGGGCTTATGTCATTTCCTCTTGACGGAAACAACTCTACTGACTTCATAGAGGATTGGGTCAAAATCTGTTTACCAG CAAAGTCAAAAGTTTTGGCAGAAAGTGAAAGTTCAGCATTTGAAGACCAATGTGGCCGATGCGAAAGGGAGGCAGTGAAT GTGTCACTAGCAAACCTATTGACATATCCATTTGTGAGAGAAGGAGTTGTGAAAGGAACACTTGCTTTGAAGGGAGGCTA CTATGACTTTGTTAATGGCTCCTTTGAGCTTTGGGAGCTCCAGTTTGGAATTTCCCCCGTTCATTCTATATGAACTAACA CATCACCATCACCATCGCTACCACCACCATCACAAACATCATCATCGTCGTCATCATCATGATCAGCATCTTCATATATA AATGTTTTACTC ................................................................................ .....................................................................i.......... .............i...............N............................................N..... ......N......................................................................... .........................................................................N...... ............................i................................................... ............N............................i...........................N.......... ....................................................................N........... ..........N..................................................................... .................................................N........N...................N. ................................................................................ ..N...........N......................................................N.......... ..........................................................N..................... Pos Score Pred ------------------------ 150 0.543 Yes 174 0.816 Yes 190 0.164 - 235 0.172 - 247 0.222 - 394 0.256 - 429 0.602 Yes 493 0.394 - 522 0.806 Yes 550 0.317 - 629 0.271 - 651 0.299 - 770 0.328 - 779 0.432 - 799 0.166 - 883 0.289 - 895 0.449 - 950 0.370 - 1019 0.038 - 1042 0.062 -

Explain the output -- Processing locus 803: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCACTTAGTAAATATATGCACATTAGGAAAGATTTCTCTACTTATATATATACCCACCGCATGGTCCCCATCAATATTCA TCACCTATCAAAGTTAAATAAGAAAAATATCATTCAAGAGGGATAATAATCATGAATCTCTTAGGAAAAATGATATATGT AGAAGGTTTTATGATGATAATGGCTACATTGTTAGTGTCCATGAGTTACGGTCATCGAGCGATGATTAATGATGTAGCCG AAGCACCCGTGTTTGACGATGTAGTCAGCCCAAACGGACTCGACAGTTCTTGGTATGATGCACGAGCAACATTTTACGGT GATATCCATGGTGGAGAAACTCAACAGGGAGCGTGTGGATACGGTGATCTATTCAAACAAGGCTATGGTCTAGAGACAGC GGCATTGAGCACAGCACTCTTCAACGAAGGGTATACATGTGGGGCTTGTTACCAGATCATGTGTGTGAACGATCCACAAT GGTGTTTACCCGGATCCGTCAAGATCACAGCAACTAATTTCTGTCCACCAGATTACAGCAAAACCGAAGGCGTTTGGTGC AACCCACCACAAAAGCACTTTGACCTCTCCCTACCAATGTTCCTCAAGATCGCCCAATACAAAGCTGGAGTTGTCCCAGT TAAATACAGACGTATTTCTTGTGCAAGAACTGGTGGTGTCAAGTTTGAAACCAAAGGAAACCCTTATTTCTTAATGATCT TGCCATACAATGTAGGAGGAGCTGGAGATATCAAGTTGATGCAAGTTAAAGGAGACAAGACCGGGTGGATAACAATGCAA AAGAACTGGGGACAAAACTGGACCACTGGTGTTAATTTGACTGGTCAGGGTATTTCATTCAGGGTTACGACGAGTGATGG GGTTACAAAAGATTTTAATAATGTGATGCCAAACAATTGGGGATTTGGACAGACTTTTGATGGAAAGATTAACTTTTAGG AGAATGATTTCAGTTTTGTATGCCAATGTATTATTTGGGTGAAATGATTAAAAATAAATGATATTATTTATACTACTTGA TTTAGCAACAAAACCTCA ...............i............................................N................... ...................................................N.................i......N... ..........i..i.....i....................i....................N......N..i........ ..................i...................................N..i...................... .......i........................................................N............... ....................................i.....................N...................N. ................................................................................ ....................................i........................................... .........................................................................N...... .........i............................N...................................N..... ............................................................................N... ....................N....N.................................N.................... ...N...............N.....N.................N.............N...................... .................. Pos Score Pred ------------------------ 16 0.510 Yes 61 0.328 - 132 0.449 - 150 0.596 Yes 157 0.260 - 171 0.531 Yes 174 0.638 Yes 180 0.724 Yes 201 0.534 Yes 222 0.455 - 229 0.246 - 232 0.588 Yes 259 0.508 Yes 295 0.473 - 298 0.604 Yes 328 0.673 Yes 385 0.399 - 437 0.528 Yes 459 0.469 - 479 0.483 - 597 0.553 Yes 714 0.366 - 730 0.669 Yes 759 0.341 - 795 0.424 - 877 0.177 - 901 0.298 - 906 0.267 - 940 0.121 - 964 0.078 - 980 0.034 - 986 0.065 - 1004 0.037 - 1018 0.040 -

Explain the output -- Processing locus 804: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATGGCTTCAATTTCCGATTCTAGCTTCTAGATTTGCACTTGATTCACATTTCGATAAGGTTTTGCTTCATAACGATTCT GTTTCTTACGAATTTGCTTAATCTAATGAGATGCTACAGATCTTAACTTTTGCAATGAAATTTATGGCAGATTCGAATTC TGCAACAACAATGACAATTAGCAACGGAGATGTCAAATCTGAAACCTGCAACAATGGCTCTTCTGAGAAGAAACCATTCA GGATCTTCGTTGGTTACGATCCACGAGAAGATCTTGCCTACCAAGTTTGTCATCATTCAATCACAAAAAGATCTTCAATC CCTGTAGAGATCACACCAATTATTCAATCAGATCTTCGAAAGAAAGGTCTGTATTGGAGAGAAAGAGGTCAGTTAGAGAG CACTGAGTTCTCTTTCTCTCGTTTCTTGACTCCTCACTTGTCAGATTACCAAGGTTGGGCTATGTTCGTCGACTGTGATT TCCTCTACCTTGCTGATATCAAGGAACTCACTGATTTGATCGATGATAAATATGCGATCATGTGTGTTCAACATGATTAT ACTCCTAAGGAGACTACTAAGATGGATGGTGCGGTTCAAACTGTGTACCCGAGGAAGAACTGGTCTTCAATGGTGCTTTA CAACTGTGGACACCCTAAGAACAAGACTTTGAGCCCTGAGATTGTTAATACTCAGACTGGTGCGTTTCTCCATAGGTTCC AGTGGCTTGAGGATGAAGAGATTGGGTCTATTCCTTTTGTGTGGAACTTTCTTGAAGGGCACAATAGGGTTGTTGAGAAA GATCCCACCACGCAGCCTAAAGCGGTGCATTATACTCGTGGAGGGCCTTGGTTTGATGCTTGGAAGGATTGCGAGTTTGC GGATCTTTGGCTTAATGAGATGGAAGAGTACAACAAAGAGAACAAGAAGGAAGCTGATAACGCAAAGTAGAAAGAAAGAA GTAAGAGGTATGTTGTTACTTACCAACACGAGTAGTGAACTTCCTTTACATAGTGTTGGCTTTGTAGAGCCAGGGATCAT TAGTCTTGT .........................N....i.......................i........i................ ..........i..................N.......................i.......................... ................................................................................ ................................................................................ .............................................................i.................. ..........................................N........N.......i............N....... .....................i...N...........................................i.......... ................................................................................ ............N................................................................... .......................................................N........................ ..............N....i............................................................ .........N...................................................................... ......... Pos Score Pred ------------------------ 2 0.165 - 106 0.122 - 111 0.804 Yes 135 0.806 Yes 144 0.755 Yes 171 0.685 Yes 190 0.304 - 214 0.626 Yes 462 0.616 Yes 523 0.124 - 532 0.123 - 540 0.730 Yes 553 0.171 - 582 0.685 Yes 586 0.103 - 630 0.558 Yes 733 0.163 - 856 0.168 - 895 0.116 - 900 0.625 Yes 970 0.199 -

Explain the output -- Processing locus 805: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAGTCGCCTTGATCTCATCAATGTTTCAAACTTTTTCTTCATTAATTCGCATCTTCTAATTCACATGTTCATGTTGCGT ATGGAATCTCCTTAGCATTAAATGCAAATTCTGACCCCAACCCATTATTTATCATTTCTTCAGCTGCACGTTTCTTTCTA TACCGTGAGAACAGAGAACATGGGAGATCATTTCGTGCTGCTGGTGGATCGGTTGATAACAGAATCCACGATTGAGGCTG CGATTCAGAGCAGGAACCAGATGTTGCAAGCTAATATACCCGTTGAGGAATGTACAATCTTGGACGATAAAACACTTGAG ATGCTGCGTAACGGAGATTTGTCAATGGCTGAGTGCAGGATCTGTCACGACGAGGACTTGGATTCCAACATGGAGACTCC TTGTTCTTGCAGCGGCAGCGTCAAGTATGCGCATAGGAGGTGCGTACAGAGATGGTGTAACGAGAAAGGTGATACCACCT GCGAGATTTGCCATCAGGAATTCAAGCCGGATTATACAGCGCCGCCTCCGTTGCTAGAGTTAGGCCATGTTCCTCTCCAT TTCAGGGGAAACTGGGGAATCTCTCAACGGGAGCATCGTTTCATCACAGTTGTACCTGCTGATTCCACTTTTATTGACCA ACCATATCCTCTTTCTTCCACCACAAGCTTCATCTGTTGCCGTTCCCTTGTTCTCATTTTCATGGCTCTTCTCATTCTCC GACATACCCTCCCTTTAGTCCTCACCGGATCAAACCTTCATGTCTTCCCTTTGTTCACGTTGTTATTTCTGAGAATACTC GGAATCATGCTACCCATCTACGTTGTCACCAAAGCAGTCGCCACCTGCCGCCGCCATTCTCGGACCTTAGACACCTCTCC CTCCGATGATTCATCTGATGAAGAAACAGATTCATGGCGCTTGCCTCAAACGCAATCTTACATTATAGGCGTACGATGAT CACTCATTCTTTTCTTTCACACACACACACAAAGTCAAATGACACTGTAAATATAACTTCTGGCTTATACATGTAAAATG TTGGATTGGTTTAACAGA .....................N...........................................N.....N........ N....................i.......................................................... ...................i............................................................ ....................N............................N.............................. i.......................N............................................i.......... ..........................N........................i............................ ..................................................................N............. ................................................................................ .............................................................N.................. .......................................i........................................ ......i......................................................................... .....N...........N...............i.........................................N.... ......................................N...............................N......N.. .................. Pos Score Pred ------------------------ 22 0.348 - 66 0.271 - 72 0.217 - 81 0.313 - 102 0.614 Yes 180 0.705 Yes 261 0.388 - 290 0.395 - 321 0.803 Yes 345 0.354 - 390 0.675 Yes 427 0.420 - 452 0.708 Yes 547 0.163 - 702 0.443 - 760 0.562 Yes 807 0.606 Yes 886 0.419 - 898 0.489 - 914 0.643 Yes 956 0.428 - 999 0.056 - 1031 0.047 - 1038 0.083 -

Explain the output -- Processing locus 806: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 807: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAAAGGGCTCAACCGGGTTCGAACCGGTGACCTCTCGATCTGCAGTCGAATGCTCTACCACTGAGCTATGGGCCCAAC TGATAAAATTATGATAAGTAAATGATATATACAAATGTAAAACCTGATTGGGTATTCGTCTCCGACTTCTTAAAATCCCA ATTCTTTCATAACAAAACACTGCTCTTCCTTCTCTCCGCCGCCGCATCGTTAAATCTCTCCGTCATCTGTAACCGGTTAA AGTATTTCCCTCAGGTCGACCTGAAAACGATGGGCAAACGAGGACCGAAGAAGCTTACAAACACTCAAGGAGGAGGAGGA CCTAATCTCAAATCTGTACTGAGACATGAGCACTTGAAGAATCTCGCTCTCTGGTCTTCCACCGGAGATACTCCTATCCC GTCTCTTGCTTCATTACTCGGTCGACGTCTCGCTGCCGACACTGAATCCACCGGAATCACTACTGATCCCGATCTCGTCT CTTGTCAAAGGTGTGAGACCATTCTTAAGCCTGGATTCAATTGCAATGTTAGAATAGAGAAGGTTTCTGCTAATGTGAAG AAGAAACGTAATAGGTGCAAGAAGTCTAACAACATCTGTTTCCCTCAGAACAATGTAGTTTACCATTGCAATTTCTGTTC TCACCGGAACCTTAAGAGAGGTACTGCTAAAGGTCAGATGAAAGAGCTTTACCCGTTCAAGCCGAAAACCGCTCGTTCGT CGCGTCCGAAGATCAAGAAAGAGATGACGATGCCTCAAGAAATCCAAAGTAACATGCTTTCTTCACCGGAGAGAAGTGTG AAAGATCAGGTGGAGGAGAAGAGTGTTGGGGATACACCGAAGCCGATGATGCTTACATTGGAGAGGGATAGGAGAATACG AAAACCCAAAAGTAAGAAACCGAGTGAACCTCAAAGTGTTCCTGAGAAAACAGTTGGTGGATCAAATAAGAGGAAGAGAA AATCACCTTGGACAAGTATGAAGGAGATAGCTGAGACAAACAAGAGTTCTAAAGCTGGAAACTTTAAAATACCTTTTCTC TTGTAATCTTTGAGAGGTTCCCAACTGATCTTTCATTGTTAACTAATAAAACCTCACGAGTCACGACGGGTATAGCTTCA TATAACAATCGTAGAGATCTTGAAG ...................................................N.................N.......... ..........N..........i............N............................................. ................................................................................ .............................i.................................................. .........................N...................................................... ................................................................................ .............................................i..........................N....... ....................................................N........................... .....................................N.......................................... .......................i.....i.......................N.......................... .............................................i..N............................... ................................................................................ .................N.............................................................. ................................................................................ ......................... Pos Score Pred ------------------------ 52 0.286 - 70 0.365 - 91 0.168 - 102 0.651 Yes 115 0.376 - 270 0.730 Yes 346 0.420 - 526 0.647 Yes 553 0.253 - 613 0.377 - 678 0.417 - 744 0.750 Yes 750 0.627 Yes 774 0.428 - 846 0.532 Yes 849 0.211 - 978 0.140 -

Explain the output -- Processing locus 808: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTTTGTCGTGAGGGTCGAGTTCTTGAAGCGCTAGCTCTTGTTGATCGGATGGTAGAAGAAGGTCATCAACCTAACGCGG TTACTTACGGAACAATTGTAAATGGATTGTGTAAGATTGGTTGTCTCGGCTTTGAATCTGCTTAGGAAGATGGGGCAAAG CCACATCAAAGCCGATGTTGTAATCTCTACTGCCATCGTTGATCGCCTTTGCAAGGACGGGAACCACATCAATGCTCAAA ACCTTTTCACTGAAATGCATGAGAAAGGTATTTTTCCCAATGTTCTCACTTACAATTGTATGATAGACTCTTTTTGTCAC TCTGGTAGATGGAGTGATGCCGACCAATTGTTGCGTCATATGATTGAAAAGCAAATCAATCCTGATATTGTTACTTTCAG TGCATTGATCAATGCATTTGTCAAAGAAAGAAAGGTCTCCGAGGCTGAAGAAATATACAAGGAGATGCTTCGTTGGAGTA TATTTCCTACCACAATCACATATAACTCAATGATCGATGGATTTTGCAAGCAGGATCGTGTAGATGATGCAAAGCGCATG TTAGATTCGATGGCTAGCAAGGGCTGCTCTCCGGATGTAGTCACTTTTAGCACCCTAATCAATGGCTATTGTAAGGCTAA AAGGGTTGACAATGGAATGGAGATTTTCTGTGAGATGCATCGAAGAGGAATAGTTGCTAATACAGTTACTTACACTACTT TGATTCATGGGTTTTGTCAAGTGGGTGATCTCGATGCCGCTCAAGACCTTCTCAATGAGATGATTTCTTGTGGTGTGGCT CCAGATTACATCACTTTCCACTGTATGCTGGCCGGCTTATGCAGTAAGAAAGAATTACGAAAAGCATTTGCAATATTGGA GGATCTGCAGAAGAGTGAGGATCATCATTTGGAGGATGAATGAAATAATGGAAGATTCAATGCCATTTTGCTTCTTGCTT GATCGATTTCGATTCTGATGTTTCCACTTGTTAATTTTTTTTTATTTGTCGTGCTTATTTAT .................................................i.............................. .....................i...............................................i.......... ..............N........................................................N........ ..............i...N....................N...................N.................... ........i.......N......................i........................................ ...........N....................................................i............... .............................N......i..........................N..N..........i.. .........i........................N..........................N.................. ...........i....i.................i............................................. ......N..........................N....................N....N.................... ........................N.............N......................................... ...................................N...N.......N...........N.................... .................N............................................ Pos Score Pred ------------------------ 50 0.711 Yes 102 0.672 Yes 150 0.545 Yes 175 0.156 - 232 0.194 - 255 0.575 Yes 259 0.327 - 280 0.300 - 300 0.254 - 329 0.539 Yes 337 0.329 - 360 0.614 Yes 412 0.177 - 465 0.620 Yes 510 0.378 - 517 0.533 Yes 544 0.187 - 547 0.278 - 558 0.729 Yes 570 0.711 Yes 595 0.167 - 622 0.249 - 652 0.509 Yes 657 0.790 Yes 675 0.606 Yes 727 0.261 - 754 0.208 - 775 0.226 - 780 0.347 - 825 0.229 - 839 0.280 - 916 0.072 - 920 0.159 - 928 0.096 - 940 0.044 - 978 0.046 -

Explain the output -- Processing locus 809: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAAATTTTTACCTTATAAATATAGTATCCAAAGGATGCCATGATGAATCAAGCTGAGAAATAACTTTTTTAGTAACACG AGAGAGATAAAGAAGAGCTAAAGAGACAGAGAGATGAAAATGATGCAAGAGGAGGGAAACCGAAAAGGTCCATGGACAGA ACAGGAAGACATACTTCTGGTAAATTTTGTTCACTTATTTGGAGATCGACGATGGGATTTTATAGCAAAAGTATCAGGTT TGAACAGAACAGGAAAGAGTTGCAGGCTAAGATGGGTTAATTACCTACATCCTGGTCTCAAACGTGGCAAGATGACGCCT CAAGAAGAGCGCCTCGTCCTTGAGCTTCACGCTAAGTGGGGAAACAGGTGGTCGAAAATAGCCCGAAAATTGCCGGGACG AACGGATAACGAGATAAAGAACTACTGGAGGACTCATATGAGGAAGAAAGCTCAAGAAAAGAAGCGTCCTGTTTCCCCAA CTTCCTCATTTTCCAACTGCAGCTCGTCATCTGTGACCACTACCACCACCAATACTCAAGATACATCGTGCCACTCGCGT AAATCTTCAGGGGAAGTGAGCTTTTACGACACTGGAGGTTCCCGATCCACTAGAGAGATGAATCAAGAAAACGAAGACGT GTACTCGTTGGATGATATATGGAGAGAGATTGATCACTCAGCAGTAAACATAATAAAACCGGTTAAAGACATCTACTCAG AACAAAGCCATTGCTTAAGTTACCCAAATCTAGCTTCACCATCATGGGAAAGCTCATTGGATTCTATATGGAACATGGAT GCAGATAAAAGTAAGATATCGTCTTACTTTGCAAATGATCAGTTTCCTTTCTGTTTCCAACACAGTAGATCACCATGGTC GTCAGGTTAAAAATTTTAAGATTTAATTCATTGAGATAAATCTGTTTTATATATATGTTATATAACTTATAAATACAATA AAACCTAACGACTGTAATAGTTGGTTGAT ...................................N....i..i.................................... .................................N.....N..N............................N........ ...................................................N............................ ...............................N.......................................i........ ................................................................................ .....................................N.......................................... ................................................................................ .........................................................i...................... ...........N......i............................................................. ...........................................N.......................N......N...N. ..................................N.......................................N..... ......................................................N......................... ............................. Pos Score Pred ------------------------ 36 0.424 - 41 0.796 Yes 44 0.717 Yes 114 0.212 - 120 0.181 - 123 0.304 - 152 0.308 - 212 0.359 - 272 0.485 - 312 0.613 Yes 438 0.288 - 618 0.781 Yes 652 0.418 - 659 0.508 Yes 764 0.280 - 788 0.166 - 795 0.368 - 799 0.482 - 835 0.240 - 875 0.114 - 935 0.065 -

Explain the output -- Processing locus 810: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACGGATTTCTGCACTCGATCTTTGTACTTCTTAGATTCATATCGAGATTTAATCAGTTTTCCATTTTAGAATCATCATT TCAATTCAAGTTGGTGTTTAAATGGAATTCTCATTGATCTCCGATGAAAATTTCATCTTAAACGATGCTCATCGATGTCC TGTTAATGATGATGCATCGCATATACCAAAAAATAATCTTAACTTTTTCAATGACAATTTAGGTCAATCTTCTCGTACTG GATGGTCTTTTTCACCAGATCTGACTGATATCTCTAATCAGCACCACCAAAATCTTATACCTTTGATCCCAAATTATGAT TCTCAAAATCAGAATTTGGATACTAATCAAAACCATTTGGTTTACAACTCTTCCTCCTATGAAATTCCATCAAACTACCC ATTCATGAGTATCAAATCCTACTCAAATATTGATACTCTTGAGCAATCTATGAATAACATAGTCAACAACGGAAAAATCC ATATGATCGATAATCCCCCAATTTTTGCAAACCCTAAGGGTATATTCGAAAATTTTCATGATTTGCAGGAATACACTATA GGGAATGAGATTGTACACAATGAAGAACTGACTAATAAGGGTTATGAGCCAACTCTTGATAAGGTAATGGGTGAACCTCA ATTATTTGATGTGCCAGTGCTAGAAGGTATCAAAAATACAACCAACGAAATCATGAATCAACTTGAAGATGATAAGATGA AAAAAACTTATGAGAATAAAAAGGAGGCGAGCACAAGCAAATATTTGAAGAAAAGTGACATCACCAAAAAAAGATGGACA GAATCTGAGGACATAAAATTGAAAGAGATGGTGGCACTTGAACCCAAAAAATGGACAAAGGTCGCAAAACATTTTGAAGG ACGAACACCAAAACAATGCAGAGAGAGGTGGCATAACCATGCTCGTCCTAACGTTAAGAAAACTACTTGGAGTGAAGAAG AAGATCAAATACTAATTGAAGTACACAAAGTAATTGGTGCCAAATGGATCCAGATCTCAGAGCAACTTCCGGGGAGGAGC TATAACAATGTTAAGAACCATTGGAATACCACAAAGCGTCGGGTGCAAAATAAAAGTGGTCGAACTGTCAACCGTGTCGG CAATAACATCCTTGAAAACTACATAAGGAGTATCACAATCAACAACGATGATGAATCTGATGGTGAACCGACAAACATTG AGAATTATCATGATGACTCTGAAGATATGTTATATGGGGAGATGAATTTGAGTCCAGAGGCTATAACGCAGACCACCAAA CCATTGACCGATGCTTCTACAATATCACCTTATATTCCGATGCCAAAAGAAAACTATACTCTGGAGGTTTGTGAATCACT GGAGGACTATCTAGAGCTGCTGCGTTGGTGGGATTAATTTCCAGTGAAGGAAATTTCTTTGATAATATGATATTTTCTCA CTATCGTGTGAGATATCTATCTATCTATTTCTTTTTAAATTAAGCAAATTTTAATT ................................................................................ .....................i.....................N....................N.........N..... .....N..N..N......................................N............................. ..........................................................N..................... ....N............................................i.............................. ..i......................................................N...................... ....N..............N.......................N......................N............. ........N...........................................N...............N.......N... .........N...............................................................N...... ...........................N......................i............................. ...............N......................N......................................... ...........................................i.................................... .......N........................................................................ ...............................................N..N........N.................... .........N..N.............N......N.......i...................................... ..........N............................N........................................ ..................................................................N............. ........................................................ Pos Score Pred ------------------------ 102 0.594 Yes 124 0.163 - 145 0.402 - 155 0.191 - 166 0.175 - 169 0.126 - 172 0.229 - 211 0.172 - 242 0.397 - 316 0.439 - 379 0.166 - 405 0.344 - 450 0.707 Yes 483 0.773 Yes 538 0.143 - 565 0.202 - 580 0.294 - 604 0.156 - 627 0.464 - 649 0.129 - 693 0.286 - 709 0.307 - 717 0.448 - 730 0.170 - 794 0.459 - 828 0.422 - 851 0.644 Yes 896 0.385 - 919 0.418 - 1004 0.777 Yes 1048 0.480 - 1168 0.350 - 1171 0.192 - 1180 0.191 - 1210 0.097 - 1213 0.124 - 1227 0.495 - 1234 0.131 - 1242 0.604 Yes 1291 0.156 - 1320 0.317 - 1427 0.064 -

Explain the output -- Processing locus 811: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAACAATTCACCGTCCGATGGAAAAAAAGATTTATAATCCCAGGAAAGCAAAAAATAAATATTCTGTTCATAACGACGTC GCTTAGTAAAAGGCCTCGTAATGTTTGGGCCTTATGATGGGCCTAAAGGGTATAATAGACAATAAGAGATTGGTTATTCG GTTGTTACCTTTTAGTTCGTCTTCTCAGAGGAGAAGTGTTTTTTTTCTCCGGCGAGGTTGGAAAAAAATGGAGGCAGCTT TGGGACTTCTCCGGAGAATGCCGCCGAAGCAGTCGGAGACGGCTCTATCTGCTCTTCTAAGCCTCATTCCTCAACATTCT TCCGATCTTCTCTCTCAAGTCGATCTCCCTCTTCAGGTGTTACGTGATATTGAAAGTGGGAAAGATTTCATCTTGTGTGA GTATAATAGAGATGCAGATTCCTACAGGTCACCTTGGTCAAATAAGTATCTTCCTCCGCTAGAGGATGCTCTTTACCCAT CTTCTGAATTAAGAAAGCTTGAAGTTGAAGCCAACGATATCTTTGCGATCTATCGTGACCAGTATTATGAAGGTGGTATA TCTTCAGTCTACATGTGGGAGGATGATAATGAAGGCTTTGTGGCATGTTTCCTTATCAAGAAAGATGGGTCAAAGTCAGG TCATGGTCGGAGGGGATGCCTGGAGGAAGGAGCGTGGGATGCCATACATGTTATACAGGTTGGTTCAGAGGAGGAGGAAA TGGCACAATATTGCTTAACAAGTACCATCATGCTTTCTCTGACAACTGATGATGAGTCTTCGGGCAAATTCGGCTTATCT GGATCAATTAGAAGACAAATGAAAATGGAGCTAGCAGTAGCTGACGGACACCTGTGCAATATGGGGAGAATGATTGAAGA ATTGGAAGGCAAACTGAGGAACTCACTTGATCAGGTTTACTTTGGGAAGACAAGAGAAATGGTTTGCACATTGCGTCCAC CAGCTGAGATTGTACAAATGAGATTGCCTGACACCTGACCTGATGCTCCTCCGATCACGCTTTATGTAAGAAGGTCTCGA TATTTGTTTGTTTTGGTTGGTCTGATCTTAAACGCTTCTCCGAGTGTTTTGTGTGGA .................i.............................................................. ....................i............N..N........................................... ...................................................................i............ .................N.............................................................. ................................................................................ ...........N.....................................................N.............. ..................................................................N............. ............i.........N.....i...............N...................i............... ..N............N......................N........N...............................N .............................i..................i..i............................ ..................N.....i...................................N........N.......... ..........................................................N..................... .................i........................N....................N................ ......................................................... Pos Score Pred ------------------------ 18 0.598 Yes 101 0.632 Yes 114 0.152 - 117 0.141 - 228 0.770 Yes 258 0.231 - 412 0.477 - 466 0.142 - 547 0.469 - 573 0.777 Yes 583 0.173 - 589 0.550 Yes 605 0.157 - 625 0.732 Yes 643 0.398 - 656 0.318 - 679 0.253 - 688 0.111 - 720 0.476 - 750 0.500 Yes 769 0.511 Yes 772 0.561 Yes 819 0.343 - 825 0.588 Yes 861 0.448 - 870 0.222 - 939 0.498 - 978 0.614 Yes 1003 0.063 - 1024 0.069 -

Explain the output -- Processing locus 812: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAAGACTATATCAAGCAGAACTTGTTAATGAGGTAAGCTCTACTAACTCTCTTTTGGTGTCCTGTTCCAACATATTTAG ATAAGCAGGAAATCTATTTAGTCTTGTATTGAGACTAAACTAATGTCAGGTTCTTCTTTCAGTCGGTGGAATATCTCACA ACGTACATATAATTTGGAACCATCCTCTCTCTCGAGCAACATGGAAACAGTTCATGGTCAACATCCATCATATCGAGATC CTCACAGTTTGATCTTTGGTTGCAATCACTACAAGAGAAATTGTAAACTTCTTGCTCCTTGTTGTGACAAGCTCTTCACA TGTATACGATGCCATGATGAAGAAGCTGATCACTCGGTGGATAGGAAACAGATTACAAAGATGATGTGCATGAAATGCCT CTTGATTCAACCGATTGGTGCAAATTGCTCAAATACTTCGTGCAAGTCGTCAATGGGAAAATACTTCTGCAAAATATGCA AATTATATGACGATGAAAGGAAAATTTATCACTGTCCTTACTGCAACCTCTGTCGAGTAGGGAAAGGATTGGGTATTGAC TACTTCCACTGCATGAAATGCAATGCTTGTATGTCACGTACCTTAGTAGAGCATGTTTGCAGGGAAAAGTGCTTAGAAGA TAATTGTCCGATTTGCCATGAGTATATCTTCACCTCCAGCTCCCCAGTGAAGGCTCTTCCTTGTGGTCACTTGATGCACT CCACATGCTTTCAGGAGTACACATGTTCACATTACACATGCCCTGTCTGCAGCAAGTCGCTTGGAGATATGCAGGTCTAC TTTAAGATGTTAGACGCATTGCTTGCAGAAGAGAAGATGCCTGATGAATACTCCAACAAAACTCAGGTGATCTTGTGTAA TGATTGTGGAAGAAAAGGAAATGCTCCTTACCATTGGCTTTACCACAAATGCACAACCTGTGGCTCCTACAACTCAAGGC TCCTTTAGATTTTTCTCCAATTTTCCTTTTCCATTGACATCATTGTAGAACCAGCCGTCTATGTAGTACATGGCTGGTAA GAAAACATTTTTGTAACATTTGATGAT ............................N................................................... ..........................................i..................................... ........................................N............N.......................... ...............................................................................N ........i....N..N...........................................i..N.....N....N..... ....................................................N......................N.... ......i.....N................................................................... ............N....N....i.......N.....................N........................... .................N.......................................................N...... ....N.................i..............i..............................i........... ......i.............................i......N...................................i ....................i...........................N............................... ............................................................N........N.......... ......................N.... Pos Score Pred ------------------------ 29 0.468 - 123 0.638 Yes 201 0.495 - 214 0.378 - 320 0.384 - 329 0.521 Yes 334 0.322 - 337 0.171 - 381 0.510 Yes 384 0.215 - 390 0.136 - 395 0.385 - 453 0.255 - 476 0.260 - 487 0.714 Yes 493 0.396 - 573 0.442 - 578 0.114 - 583 0.552 Yes 591 0.449 - 613 0.312 - 658 0.458 - 714 0.424 - 725 0.307 - 743 0.782 Yes 758 0.749 Yes 789 0.584 Yes 807 0.665 Yes 837 0.626 Yes 844 0.490 - 880 0.551 Yes 901 0.523 Yes 929 0.164 - 1021 0.062 - 1030 0.112 - 1063 0.031 -

Explain the output -- Processing locus 813: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTCCATATCATATTAAGCCACCATAAGCCCAATAACTTACAGTGAAGCCCATTTAGTACTGAGCCCGTTTCGTACTTGTA AAGTAGTTAAGTAGCGAATAAAATATTGCCACGTATTCGTTTCAAGCAAATTTGACTAACTTATTTTTCAGTTTTGGAAA TTTCGATTCGGTTACCATATCAAATTTGGGCGGGCGAGGAATAAACCAAACCGGTCGAGAGTTCACGAGCCACGGAGAGA TCTTGGCGATGAGTTGTCGGAGATTCTTCTCGATCCCGGTCCTTTTGGTGATTCTGGTGATGGGTTTCGTGTATTACGTC ACACTGTTTGTTTTCATCGATGATTGGGTTGGTTTACAAAGCTCAGCTGGGAAATTAAACGCCTTACTCTTCAGCTTATT GGCGTCTCTCTGTCTCTTCTCGCTCTCAATTTGCGTTCTTGTAGATCCAGGTCGTGTTCCTGCATCTTATGCTCCTGATG TTGAGGACTCTGGCTGGTCCAATAGTAACGTTACAGAAACAAGGAAATGTGATAAGTGCTTTGCATATAAGCCTCTTCGG ACTCACCATTGCCGGGTTTGCAGAAGATGCGTCTTGAAGATGGACCATCATTGCCTCTGGATAAATAACTGTGTTGGTTA TGCAAACTATAAGGCTTTCTTCATTCTTGTGTTTTATGCAACCGTGGCTTCCATCTACTCTACAGTGTTGCTGGTTTGCT GTGCATTCAAGAACGGAGATTCCTATGCGGGAAATGTTCCGCTCAAAACTTTCATTGTTTCTTGTGGGATCTTCATGATC GGACTAAGCATAACACTTGGGACTCTCCTTTGTTGGCATATCTACCTCATCACACACAATATGACCACCATTGAGCATTA TGATTCAAAGCGGGCCTCATGGTTAGCTAGAAAATCAGGACAAAGCTATAGGCATCAATTCGATGTTGGCTTTTACAAAA ACCTCACCTCGGTGTTAGGCCCTAACATGATCAAATGGCTATGCCCCACATTTACTAGGAATCCTGAAGACGGTATTAGC TTTTCTGCATCGAGAGACAGCTAGCAAAATATTTCAATTCTGGACACTAGAAAATGATTCAGAGACCTTAATCCCTTTCT CATCTCATCTATGCGAGTGTAAATGATTCGGAGACCTTAGATA ................................................................................ ................................................................................ ................................................................................ ........N..................................................N.................... ...................N............................................................ ....................................................................N........N.. ..............................................N................................. ..........................N............N.......................................N ...................................N............................................ ........................N........N........................................N..... ............................................................N..................N ..................i...........................................N................. ..........................N.......i.....N....................................... .....................................................N.......................... ..........N...........N.................... Pos Score Pred ------------------------ 249 0.296 - 300 0.240 - 340 0.300 - 469 0.148 - 478 0.174 - 527 0.461 - 587 0.171 - 600 0.386 - 640 0.131 - 676 0.065 - 745 0.376 - 754 0.424 - 795 0.371 - 861 0.349 - 880 0.291 - 899 0.596 Yes 943 0.331 - 987 0.356 - 995 0.501 Yes 1001 0.197 - 1094 0.182 - 1131 0.046 - 1143 0.052 -

Explain the output -- Processing locus 814: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGACGATGTATCGTGAACAGACCAAACGACGACGTAGTGAATACAATCGTAATCGTATTCGTATTCGTGAATGGGTCAAA CGCCGTCGTTCAGAAACTTAGTTTAAACGATGCCGTGTTTCTAATAGACCAAAAACGACAGCGTATTATTTTATTTTTTC TAATTTCCCATTAATGTTGGAATGCTCGCGTGACAGAGCAGTGACGATGGAGGACGAGCAAGACGCGCATCATCGCCGGC AGATCTGCGACAACTGCGATCGCCCAAATGCGATTTGTCTCTGCCACGTCTTACCAGCGGATCTAATTCCGACGAATACA GAAATCATCATCCTCCACCATCCACACGAGTCACGCCACAAGCTCAACACCACTCCTCTCCTTACCAAATCCTTACTAAA CGTCACCAGAATCCCCGCCCGCCGTCTCCTCCGCCGTCACATCTCCACCGCCGGAGGAACCGCACTCCCAGCACCGCCAA CAATCTACCTCTTCCCTTCTTCTCCTTCCTCTCCCGCCGTCACTATATCCGAATTCAAATCCCTAAACCTCTTAAACCAT CGTGAGATCTCAAATCCCCCGCCGCTGAGACTAATCGTATTCGACGCGACGTGGAAACACGCGAAGGAGATGGTCAAAGC TAGCGAGGTGGTGTTAAGAGAAGCAGGAGCGGTTAGAGTGTGTTTGGATACGGAAATAGACGCATCAGTGAGTGGAGGAA CCATCTACGATTCAGAGCTTGTGCTAAGGAAGGAACCATTCGGAGGATGTGTAACTACGGCGGAGGCTGTGGCACGGTGC TTAGGAGCTATTGAACCTGACGGTGAAGAAATAGAGAGGAAGCTGATTAGTGTTTTGAAAGAGATGGTTAGATTTCAATC TAAGTACTTGAAGCCTATGAAACCAAGACCTAAGTTATTGAAGAAGAGATTTCAGAATCAGCAACCACTTGAACAAGAAG AAGAATGAGTTAAATCCTGAAGTTTAGCAAAAAAAAATTGCTCTATGACATTAGTCCATTGTATTAATTTGTTCTTCTAT ATCAATAATTAGTTAATTTACAAAATA .....N................................................................N......... .............................i.................................................. .............N.......N........................i................................. ...........................N.................................................... ................................................................................ ................................................................................ ................................................................................ .....................................................................i.......... ................................................................................ ..............................................N................................. ...............................................................N................ ................N............................................................... ....N.......................................N................................... ........................... Pos Score Pred ------------------------ 6 0.470 - 71 0.213 - 110 0.571 Yes 174 0.493 - 182 0.316 - 207 0.763 Yes 268 0.442 - 630 0.739 Yes 767 0.280 - 864 0.243 - 897 0.157 - 965 0.253 - 1005 0.067 -

Explain the output -- Processing locus 815: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTCGTGTCTCAATACGCACGTTTAGGACTTAGAATGGCGTTTTCGTAAATACTAACTTATAGTCGTAATTAAAAATTGT ATATTCATTTTCCCTCTATAAATGACTCTGTTTCGTTTCCTGCTTCACTTAAGAAGTAGCAGAACAATGGCGACTTCTCA GCAATTTTTAAACCCTACACTCTTCAAATCCTTAGCTTCCTCAAACAAAAACTCATGTACTCTCTGCCCATCTCCTTTCT TGCAACTCAAGTCCGCCTCCACAATTTTCAATTACAAACCACTTACTTCCTCCTCCGCCACGATCATCACACGCGTCGCT GCATCATCCTCCGATTCAGGCGAGTCAATAACCAGAGAGACTTTCCACGGCCTCTGCTTCGTCTTGAAAGACAACATCGA CACCGATCAAATAATCCCCGCCGAGTACGGCACTCTCATCCCTTCGATTCCAGAAGATCGCGAGAAACTCGGCTCTTTCG CGCTTAACGGCTTACCAAAATTCTACAACGAACGTTTCGTTGTTCCAGGAGAGATGAAATCAAAGTACTCAGTCATCATC GGCGGCGATAATTTCGGTTGCGGATCTTCCCGCGAACACGCTCCAGTTTGTCTCGGCGCGGCGGGAGCTAAAGCTGTGGT GGCGGAATCGTACGCTAGGATCTTTTTCAGGAACTGTGTAGCTACAGGTGAGATTTTCCCGTTGGAATCGGAGGTTAGGA TTTGCGACGAGTGCAAAACAGGGGATGTGGTGACAATCGAACACAAGGAAGACGGTAGTAGTTTGCTGATCAATCATACG ACGAGGAAAGAATACAAACTGAAACCGCTCGGTGATGCCGGTCCGGTGATCGACGCCGGTGGAATCTTCGCTTATGCAAG AAAAGCCGGCATGATTCCTTCTGCTTGAATGTAATCGGATCCATAATTTATCGGTTCTGAGTTTAATCCGGTTTGGTTTA TTTGGTTCGAGAACCGGTACTAACGAAATAAATTATTACCAAACCAA ..................................i............................................. .....................N............................................i............. ......................................................N......................... ................................................................................ ................................................................................ ................................................................................ .....................................................i.......................... ................................................................................ ................................................................................ ........................N....................................................... ..................................N......................................N...... ..........N.................N................................................... ............................................... Pos Score Pred ------------------------ 35 0.651 Yes 102 0.420 - 147 0.588 Yes 215 0.436 - 534 0.727 Yes 745 0.193 - 835 0.280 - 874 0.177 - 891 0.452 - 909 0.239 -

Explain the output -- Processing locus 816: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATAAAAATTTACTACATATGTTGATATTATACTTAGGTTAAAAAAAAGAAAGATATCCTATGTGGCAACCACCTACAAA CTTTATTATAAATATAAGTAGATGGCAAGGCGTGTGGTTGCCAAACACAAAGAAAAACATTCGACATTACAGAAAAGAAA AAACTATAAGAAAATCTTAATTAATAAAATAATATATTCAGGAGGGCGAGAGAGAGAGAGAGGGATGATGAAGGAAGAAC AATGGGCACCAGTGATAGTAATGTTAATATCAAGTGTTGCAATGGGATCAGTGAACGCTCTCGTTAAGAAAGCACTTGAT GTTGGTGTTAACCATATGATCTTTGGTGCTTATAGAATGGCTATTTCTGCTCTAATCTTGGTTCCGTTTTCTTATATTTG GGAGAGGATAGAGAATGCGCAAAACCTAAAGAGCAAAGCAGGCGTGCTCAAAGTAATGGGAACCCTAATTTGCATAATGG GAGCAATGTTGTTGACATTTTACAAAGGCCCAGAGTTATCAAACCCTCATTCTCATCCACAAGCTCGTCACAACAACAAC AATAACAATGGTCACGACCAAACAAAGAAATGGCTTCTAGGCTGTCTTTATTTAGTCATTGGGACAGTGTTGTTGTCTCT ATGGATGTTGTTTCAAGGGAAATTAAGCTTTAAGTATCCTGGAAATAAATACTCAAGCACTTGTCTCATGTCAGTCTTCG CATCATTTCAATGTGCTATTTTGAGTCTTTATAAAAGTAGAGACGTCAAAGATTGGATCATTGAAGATAAATTCGTCATT TTGGTCACCCTTTACGCTGGAATAGTGGGACAAGCGATGTCGACAGTGGTAACGTCATGGTCCATAAAAATGACAGGAGC AGTTTTCGTCTCAACATTCAGCCCCGTCAGCCTCGTCGCCGCTACGTTATTCGATTTCCTCATCTTACACTCTCCTTTAT ACCTTGCATACTTGGATCAGTAGTGACTATAACTGGCCTCTACGTTTTTCTTTGGGGAAGGAAGAATGAGACAGATCAGT CTGTTTCGAAGACTTTGAACAGTTCCCAGTTTTCTCAGAATA ..................N.........................................i................... .....................N.......................................................... ................................................................i..i............ ...............N....................i........................................... ..............N........................................N....................i... .....N.......................................................................... .......i.....................i.................................................. N...N..............................................................i............ ..........N..................................................................... ....................................i...................N............i.......... ................................................................................ .................................................................N.............. .......................................... Pos Score Pred ------------------------ 19 0.161 - 61 0.586 Yes 102 0.412 - 225 0.501 Yes 228 0.563 Yes 242 0.416 - 261 0.307 - 282 0.337 - 319 0.119 - 336 0.288 - 357 0.638 Yes 415 0.219 - 456 0.447 - 477 0.646 Yes 486 0.367 - 568 0.562 Yes 590 0.637 Yes 641 0.188 - 645 0.087 - 708 0.509 Yes 731 0.375 - 837 0.565 Yes 857 0.393 - 870 0.644 Yes 1026 0.077 -

Explain the output -- Processing locus 817: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTCCATACATAATTTTCATTGTCTATATATATATTGATCTATTTGTAATTGTAACTCTTGAAAGTTTGGAAAATATTT GTAATAAGAGATAAACAATGATATGGGCCGATATTTGCGAAACTGATTTCAAAGGATTCTTCTTTATGGGCCTATAGAGT TTTGGGCCTTTCTACCAAAACTTTCGCGACAAGCGATATGTTAGGGCACAAGGACAATTCGAAACCTGAGAAGAGAGCTA GAGCTTGTCAGATGTTAGGATACTCCTATCCTTGTTTCCAAACGGGATTACGGTTAACGACGGCGATGGCGCCGGAAATT CACCTGAAACCGAAGAACCCTAGAATTTTATGCCTTCATGGATTCAGAACTAGTGGTCGGATTCTCCAAGCCGGTATAGG TAAATGGCCGGACACAATTCTCCGAGATCTCGACCTCGATTTCCTTGACGCTCCTTTTCCGGCCACCGGGAAATCGGACG TCGAGCGTTTCTTTGACCCACCGTATTACGAGTGGTACCAGGCTAACAAGGGGTTTAAGGAATATAGGAATTTTGAAGAG TGTTTGGCTTATATAGAGGACTACATGATAAAGAATGGACCTTTTGATGGTCTTCTTGGTTTTTCTCAGGGAGCTTTTCT AACTGCAGCTATTCCTGGAATGCAAGAACAGGGATCGGCTCTAACTAAGGTGCCAAAAGTCAAGTTTTTAGTTATCATTT CTGGAGCGAAAATTCCAGGGTTGATGTTTGGGGAGCCTAAAGCTGCTGTTAATGCATTCTCATCTCCAGTTCGTTGCCCA TCACTCCACTTCATAGGTGAGAGGGATTTCCTGAAGATAGAAGGTGAAGTGCTTGTAGAATCATTCGTAGAGCCAGTGGT GATCCATCATTCAGGTGGACACATTATACCAAAACTCGATACTAAAGCTGAGGAGACAATGTTGAGTTTCTTCCAGAGCA TTAGGCAGATGCTTTCTGATGAATCAGGTTCTGTAAGAAGCTTGATGTGACTGAGAGCTACTCCAGTATGGTTTACTTTA GATTAGATGATCTTGCAATTTTATAAAATTGTGAGATGTGATTATTCTTCTGGTTTAGTCTTTATCCAG ................................................................................ .................N....N..........................................N.............. .....................................N.......................................... ...........N.....................................................N.............. .............................N.......N.......................................... ...i............................................................................ ................................................................................ ........................i.........N...........N................................. ...................N............................................................ .......................N...........................N............................ ................................................................................ ..........................................................N..................... ........N.........N.........................N......................N............ ......N............................N................................. Pos Score Pred ------------------------ 98 0.368 - 103 0.484 - 146 0.386 - 198 0.389 - 252 0.247 - 306 0.480 - 350 0.252 - 358 0.305 - 404 0.655 Yes 585 0.654 Yes 595 0.281 - 607 0.087 - 660 0.496 - 744 0.181 - 772 0.121 - 939 0.360 - 969 0.241 - 979 0.328 - 1005 0.115 - 1028 0.050 - 1047 0.040 - 1076 0.046 -

Explain the output -- Processing locus 818: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGAAGATACAATTTAATTTCTCGAAATATTCATTTACTTTGTGCTAACATATTGGGCTAGGCCTTATCTCTATTCGGC CCATCATCTAGAAACGTCTAAGATACTGACGCGTGTTTTATGGCTCCGGTAGAATTGAAGAAATCACCAGAGACTACAAT GGCTGCCTCCACCGTCGTAACCACCGTGACACACGGAGCTTCATCTATACTTCCTTCACGAACTGCATTAGCAGTCTCTT CCCGGAGGTTTTCTTCATTCTCGCCGTCGAGGTTGGACTTCAGTAATCTGCGGCGGAGAGTCAATGCACCATCGCCGAGG CGCCTCGTGGTTCGCGCCGCTAGAACTGAGTCAGCCGGAGTAAAACTGGGAGCTAGAGCTCCCAATTTTGAGCTTCCGGA GCCGCTCACTGGAAACTTATGGAAATTAGAAGATTTTGAACTGTATCCGTCTCTACTGGTAATGTTCATCTGCAATCATT GCCCGTTTGTGATTCATTTAAAAAAAGACATTGTAAAGCTATGCAATTTCTATATGAAGAAAGGTCTTGCTGTTGTTGCA ATATCTTCAAATTCTGTGGTAACTCATCCTCAGGATGGGCCTGAGTTCATGGCAGAGGATGCAAAAGTTTTCAAATACCC ATTTCCTTACTTATATGATGAGTCACAAGAGGTTGCAAGAGAATTTGGAGCTGTTTGTACACCTGAGTTTTTCTTGTATA AGAAGGATGGTCGTAGACCATTTGAGCTGGTCTATCATGGTCAATTTGATGATTCCCGGCCTAGCAGTAACTCACCAGTA ACCGGGAGGGATCTAAGCCTGGCGATTGATCTTTCCCTTAGCTGTCAACCAATACCATCAAATCAAAAGCCAAGTGTTGG TTGCAGCATAAAGTGGCACCCAGAAACAAAGTCATGAGCTAAGACATGATTGTACTTACAAATAGCATTATTTTCAGGCA AGTCGCTTGTTCTATATCAATTATGTTCCATAGACTCAGGTCCGTAGTATGTCGGA ................................................................................ .......................................i......................................i. ................................................................................ ...............................................................N................ ................................................................................ ..................i..........................................N.................. ........................................N............i.......................... ..................................N.............i.........N..................... ..............N..N.............................................................. ......i.............................N...........N............................... ................................................................................ .................................N...........N.................................. ......................N.........................N....... Pos Score Pred ------------------------ 120 0.551 Yes 159 0.541 Yes 304 0.303 - 419 0.544 Yes 462 0.399 - 521 0.400 - 534 0.650 Yes 595 0.099 - 609 0.596 Yes 619 0.133 - 655 0.124 - 658 0.277 - 727 0.615 Yes 757 0.131 - 769 0.366 - 914 0.399 - 926 0.240 - 983 0.035 - 1009 0.105 -

Explain the output -- Processing locus 819: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCACTAAGACCTATTATGGTATGTTGTATTGTATGACCCATTTTTTCAATTATTTTTTTCGGCCTAAACCAGCCTTTTAC AGAAGCAAAAGGAAAAACTGAAACCCGAGACATCTAAACCGGATTAAACCGAATACCATGCCATTTCTTTTTGATGAGTC ATCATCACCATCCTTTAGATCCAGAAATCAAAATTGCTTACTGCTTCATTTGTTCTGCTGTTCCCGGCCAATGAAGATTC TCGTCGCCGTTAAACGTGTCGTCGACTACGCCGTCAAGATCCGAGTCAAACCCGATAAGACTGGTGTAGAAACCCAAAAT GTGAAGATGTCAATGAATCCATTTTGTGAGATCGCGCTTGAGGAAGCGCTTCGAATCAAAGAAGCTGGTTTTGCTAAAGA GGTTATTGCAGTGAGTATTGGACCTTCACAATGTGTGGATACACTTCGTACTGGTTTAGCTATGGGTGCTGATCGAGGAA TCCATGTTGAGACCAATTCTATTTTTCTTCCTTTGACGATTGCTAAGATCTTGAAGAGTTTAGCTGATGTTGAGAATCCT GGATTGATTTTCTTAGGGAAACAGGCGATAGATGATGATTGCAATCAAACAGGACAGATGGTTGCAGCTTTACTTGGTTG GCCACAAGCAACGTTTGCATCGAAGGTTGTGTTGGATAAAGATAAGAATGTTGCAACCGTGGATAGAGAAGTGGATGGAG GTCTTGAGACTCTTAATGTAGACTTACCCGCTGTGATAACAACTGATTTGAGGCTGAACCAACCAAGATATGCATCACTC CCTAACATAATGAAGGCAAAATCAAAGCCTATAAAGAAAATGACGGTGCAAGATCTGAAAGTAGACATTAAATCCGACAT AGAGATCTTGGAAGTCACGGAGCCTCCAAAGAGGAAATCTGGGGTTATGGTTTCTTCGGTGGATGAGTTGATAGATAAAC TTAAAAATGAAGCTCATGTTGTTTGAAGGAAGAAACATTTGTCTTTTTCCCATTCTGCTACACACTTCTTTCTTCTTATC TACGTTGCTGTTTCAGGCATCAAGTGGAAGTAAATAAATAAATCA ...............i....N...........N............................................... .........................................................N...............N...... ......................................................................i......... ..............................................................................N. ......i.....i................................................................... ..............................N..............................i.................. ...N..............................................................N............. ...............................N..N......................i...................... ...............................................N..........................N..... ...............N.....................................................N.......... .........i.............................i........................................ ..............................................N...............N................. ......N........N................................................................ ............................................. Pos Score Pred ------------------------ 16 0.523 Yes 21 0.243 - 33 0.243 - 138 0.149 - 154 0.264 - 231 0.652 Yes 319 0.294 - 327 0.793 Yes 333 0.549 Yes 431 0.337 - 462 0.548 Yes 484 0.170 - 547 0.304 - 592 0.184 - 595 0.135 - 618 0.832 Yes 688 0.241 - 715 0.157 - 736 0.191 - 790 0.234 - 810 0.553 Yes 840 0.617 Yes 927 0.218 - 943 0.053 - 967 0.189 - 976 0.203 -

Explain the output -- Processing locus 820: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATGTCGTAGTGACAATGTTTATCTGATTTCCTAGATTATAAGAGTTGTAGTTGGTAATAGACTAAGAAAAGCTTTTACA TCTTTTGGTCAAAAGGTTTGATTTTTAGTGCTATATATGCAGCTCAATTCCATGGCGCCTAGATGGAAATGGAAAGGAGC TGAAGCAAAAGCTCTTGCAGAACCTATTTCAAAATCAGTCTCAGAGCTCCAGCTCTCTCTAGCCGAAACAGAGTCCTCGG GAACCCTCTCAAGTTGCAATGTGCTTCTAGCGGTTGAGCCAGAGCAAGCTGAGCTTCTTGACCGTTGTTGTTTTGGCAGA CTCGTTCTATCGGCAGAGAAAATCAAGAAATGGATTCAGTTATCTTTTGAAGAAGCTTTCTTTTTGCATTACAATCTCAA ATGCATCAAAATCTCACTTCAAGGTCGTTGCTTGGAGAATGAAGTAGACACATGGCTCTACATGAAATCAAAAAGACCAA ACTTTCCAATGTTTTTCAAAGCTTATTCACATTTACGATCGAAGAACTGGGTTTTGAGATCAGGGTTGCAATACGGTGTG GATTTTGTGGCATACCGTCACCATCCATCTCTTGTCCACTCTGAATACTCGGTTTTAGTTCAGTCTGGTGACAGTGATCG GTTAAGAGTGTGGTCAGATATCCATTGTGCTGTTAGGTTAAGCGGAAGTGTTGCGAAAACGTTGTTGACTCTCTACGTTA ATGGTAACTTCAAAGGAGAGGATGTGAATCTACTTGTATGTTTGGAGAACTTCACAGTGGAAGAACAAACGATAAGTAGA TGGAGCCCAGAACTCAGTCGTGAAGATCAATCCACAAATTCAAAACAACATGTTCCCAACGTGAGTAATTTGAACACTCT TTGAGGAACCATTCTCGGTAACTCATGTCTGGTTTGAATTTTTCATATCAACTTTAAATTAATTTCTACTCTATATAAAG ATGAGTAGCATTTCTTTCAACTA ....................................N..............i..........i.....i........... ................................................................................ ..................N............................................................. .............................i.................................................. N.....................................i............i.........N.................. ........N....................................................................... ................................................................................ ................................................................................ N....................N...............N.........................................N .................................................N.............................. ........................N....................................................... N...................... Pos Score Pred ------------------------ 2 0.271 - 16 0.180 - 117 0.331 - 132 0.584 Yes 143 0.646 Yes 149 0.682 Yes 259 0.401 - 350 0.522 Yes 401 0.464 - 439 0.588 Yes 452 0.659 Yes 462 0.343 - 489 0.265 - 721 0.236 - 742 0.367 - 758 0.385 - 800 0.490 - 850 0.088 - 905 0.150 - 961 0.084 -

Explain the output -- Processing locus 821: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTCACTGGACAGCTTCTTGGACTTGATGTTGGTAGCGTCCTCGAAGTTACCAATTGTTTTCCTTTCCCGGTATGTTGA TGAATTTTACACGCATTGTGAGGATTTAAGAGCTTGTTCATGATTGATGTACATGTGATTTACTGGAATTTGTATCTTAA ACGAGGACAGGTCAGGGATGATGATGAAGAAATTGAAGCTGATGGTGCTAATTATCAGCTTGAGATGATGAGATGTCTGA GGGAGGTTAATGTTGACAACAACACTGTTGGCTGGTATCAATCTACAGTTCTTGGTTCGTATCAGACTGTGGAACTGATT GAGACCTTCATGAATTACCAGGAGAATATCAAGAGGTGTGTGTGCATCATATATGATCCCTCTAAAGCTGATCTAGGCGT CTTAGCTTTGAAGGCTTTGAAGCTTTCAGATTCCTTTATGGAGTTGTACCGAGGTGGAAACTTTACTGGCGAGAAGTTGA GAGAGAAAAATTTCTCCTGGATGGATATTTTTGAGGAAATACCTATCAAGGTTTCAAATTCTGCCCTTGTCAGTGCCTTT ATGACCGAACTGGAGACTGATACACCTGTCTCACAGGGCGATTATGATCGTCTACACTCATCAACCACTCCTTTCCTTGA GAACAATATGGAGTTTTTGATTAAATGCATGGATGATTTATCTATGGAACAGCAAAAGTTCCAGTATTACTACCGGAACC TGTCTCGTCAGCAAGCACAACAGCAAGCCTGGCTCCAGAAGAGAAGAACGGAGAACATGGCTCGTAAATCAGCTGGAGAG GAGCCTTTACCAGAAGAGGATCCTTCAAACCCAATCTTTAAGGCGATCCCTGAACCATCTAGGCTAGAGAGTTTCCTCAT CACAAACCAAGTCTCAAACTTCTGTGGCCAAATCAATGGAGTGGCTGGCCAGAACTTCAGCAGGCTTTACCTGACCAAAG CATTGCACGACAACTGATTGCAAAGACTACCCAAGTTTTTGTTTTTTGAGTCAGAAAGTGAAAGACTTGGTGGTAGAATG GATCCTATATTTGTTTTTCGTGGATGTTTTTGTTTT ...........................N.............................................N.....N .......................................i......N.....N........................... .................N..N..N.................N......................i..N....N....... .........N...................................................................... .........i..........................................N........................... .....................................N.......................................... ....................i........................................................... i..........................................N.................................... .......N................i...i...i..........i.................................... ........................................................i....................... ................................................................................ ...................................N............................................ .............................................................................N.. .......................N............ Pos Score Pred ------------------------ 28 0.327 - 74 0.303 - 80 0.177 - 120 0.652 Yes 127 0.401 - 133 0.249 - 178 0.184 - 181 0.373 - 184 0.223 - 202 0.093 - 225 0.578 Yes 228 0.277 - 233 0.404 - 250 0.239 - 330 0.650 Yes 373 0.260 - 438 0.475 - 501 0.583 Yes 561 0.520 Yes 604 0.153 - 648 0.279 - 665 0.557 Yes 669 0.585 Yes 673 0.709 Yes 684 0.524 Yes 777 0.736 Yes 916 0.425 - 1038 0.058 - 1064 0.039 -

Explain the output -- Processing locus 822: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAGGAGATTACTACACAATTAGTGCTGTGAGAATGAATATACGGACATGCTTTGTTAAAAAGATTGAAACTTTTGCTTC ATTTTGATCAAAAGATTTTAACTTTCGATTCTTTTAGTAAATGGGTCGCTCTTTGTTTGGACTATAATGATCAGACTGCT CTTGTCTTGTGTGATTAGGAATCTAAAAATGTCACGTGGAAGAGGAGTTCCTATGATGGACCTGAAACGAAGTTATGATG TTGAAGACAGGGTGGTGTCCGTGTCTATACCATCAATAATCGAAGCTGATGAGGCTGATCTGTGGCCACTACCTGAGATT GATACCAAGAAGTCGAAATTTCCTTGCTGTATAGTTTGGACTCCTCTTCCTGTTGTCTCTTGGTTGGCTCCTTTCATTGG TCATATTGGACTTTGCAGAGAAGATGGAGTCATTTTGGACTTTGCTGGATCTAACTTCATCAATGTTGATGATTTTGCAT TTGGTCCTCCTGCTCGCTATCTCCAACTCGATAGAACCAAGTGTTGCTTACCACCAAATATGGGTGGACATACTTGCAAG TATGGATTCAAACACACCGACTTTGGAACAGCGCGTACATGGGATAATGCACTGAGCTCGAGCACACGTAGCTTTGAGCA TAAAACCTACAACATCTTCACTTGTAACTGCCATTCGTTTGTTGCAAACTGTTTGAACCGTCTTTGCTATGGTGGCTCAA TGGAGTGGAATATGGTGAATGTTGCTATTTTACTTATGATCAAAGGGAAATGGATCAATGGTTCATCAGTAGTCCGCTCG TTTCTGCCATGTGCTGTGGTCACGTCTTTGGGGGTGGTGCTTGTCGGCTGGCCATTCCTCATCGGTCTCTCTTCGTTCTC GCTACTACTCTTTGCCTGGTTCATAATTGCTACTTACTGTTTTAAGAACATAATTACTTGAAGCAGTATGAGAGAAATAT AAATATGATGTATCACCAAAATTATGTTAATCTTAAGAATTGTTCTCTTTTCCTTTCGGCACCATCTAGCTTTCAACTCC .................................N.............N................................ ........................................N.........................N............. ............................i.......................i..i..................N..N.. ................................................N............................... ................................................................................ .......................N......................................N.....N........... ...........................................................i.................... ....................................................................N..........N ...........i......N................N.............i.......N...................... ........N....................................................................... ...................................................................N............ ....N..N...............N........................................................ Pos Score Pred ------------------------ 34 0.298 - 48 0.195 - 121 0.149 - 147 0.409 - 189 0.782 Yes 213 0.527 Yes 216 0.580 Yes 235 0.161 - 238 0.188 - 289 0.093 - 424 0.370 - 463 0.263 - 469 0.311 - 540 0.870 Yes 562 0.433 - 599 0.297 - 607 0.393 - 709 0.350 - 720 0.497 - 732 0.615 Yes 739 0.115 - 756 0.236 - 770 0.714 Yes 778 0.387 - 809 0.314 - 948 0.162 - 965 0.203 - 968 0.096 - 984 0.047 -

Explain the output -- Processing locus 823: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTCAGATTCTTCAAGGTAAATTGAATATCTCCCTCAGAAGATGAAGATTTATCGAATCATCAAAGATACTTTGCGAGCT CTTCGAGTATAAATGTTCTGATCTTTGTTGTCTTTGTTTATGTTGATCACAATTGGTGTTAGGAATTCGATTGCTTTTGC AAGAATGAGACATGTGTACGTTGGGAATTTCGACTATGATACTCGCCATTCGGATCTTGAAAGATTGTTCAGCAAGTTCG GGAGAGTGAAGCGAGTTGATATGAAGTCTGGTTATGCTTTTGTGTATTTTGAGGATGAGCGCGATGCTGAAGATGCAATC CGTAGGACTGACAATACCACTTTTGGATATGGGCGACGCAAGTTATCAGTTGAATGGGCCAAGGACTTTCAGGGTGAACG TGGGAAGCCTCGTGATGGAAAGGCAGTCTCGAATCAGAGGCCTACAAAGACACTCTTTGTCATCAACTTTGACCCTATTC GCACAAGAGAGCGTGATATGGAGAGACACTTTGAGCCATATGGTAAAGTTCTCAATGTTCGCATGAGGCGCAATTTTGCT TTTGTTCAATTCGCAACCCAGGAAGATGCAACCAAGGCTCTTGATTCTACACACAACAGCAAATTACTGGATAAGGTTGT TTCTGTTGAGTATGCCTTGAGGGAGGCTGGTGAAAGAGAAGATAGATATGCTGGCAGTCGAAGAAGGAGATCTCCTAGCC CAGTGTATCGGAGGCGTCCTAGTCCTGATTATACCCGTCGTCGCAGTCCTGAATATGACAGGTACAAGGGTCCTGCTCCT TATGAAAGACGTAAGAGTCCAGATTATGGGCGTCGCAGTTCAGACTATGGTAGAGCAAGAGCTAGGAGTCCGGGCTATGA CAGAAGCCGGTCCCCGATTCAAAGAGCAAGAGGTTGAAGATGTTGTTGGGCGATGGATTTTTAGAATGTATGGGCATGGT ACAAAAACAAGTTCTTAGTACTGAGTCTTTACCGCTTATGACTTGAACCGTATGTG .........................................N...................................... ............N..........................i........................................ ....N......N.......................N............................................ ....................i............N....................i........N........N....... ............................N........................N.......................... ..............N................................................................. .................i.....................N..............N.......i................. .........................N...................................................... ...........N...................................i................................ ......................................................i......................... .......................................i............N............N...N.....N.... .....................................N.............N.... Pos Score Pred ------------------------ 42 0.205 - 93 0.247 - 120 0.728 Yes 165 0.426 - 172 0.378 - 196 0.107 - 261 0.806 Yes 274 0.235 - 295 0.554 Yes 304 0.334 - 313 0.349 - 349 0.457 - 374 0.394 - 415 0.198 - 498 0.809 Yes 520 0.181 - 535 0.290 - 543 0.721 Yes 586 0.332 - 652 0.288 - 688 0.539 Yes 775 0.690 Yes 802 0.342 - 826 0.638 Yes 847 0.630 Yes 877 0.381 - 920 0.519 Yes 933 0.087 - 946 0.177 - 950 0.057 - 956 0.044 - 998 0.034 - 1012 0.048 -

Explain the output -- Processing locus 824: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTAATTGGTTTCTAACGAGTTTTTCATTGATAGACTTAACATTTGGCAGATTTTATTAAGTTTTGGGGCTAAAAATGG AAATTCAAAGAACGGCGATAGGATATATATAAAGGTCTCCATGTCGTTATGTTTTTTAATCAAAACATTTTAACCAAACA GCAAAATCAAAATCAAAATCCAAAGCTAAAAAACAGATACTATCAGATAACATCAAAATATATAGAGACACAACAAATAC AACATTTATCTATACTAGAGTTTATTACTATGGCTTTTGATGAAGATATGAAAATGGCGTCTTTCTTAAGAGAAGAAGAA GAACAACACTATTCTTTATCAAGACTCTCCGTTTGTTCCAACTACGACGGCGATGAAGCCGACGGTGAGTCTTCTGCTTC CGACGAGAAACGTGTTGTCCACGGCGGAGGAGAGAAATCCATGGAGGAGCTAAATTTTTCAGATTCTGATAAAGAATCAA CCGGTTGTCAATCTCTCCCGGCGACACCTCCGAGACGGAGACGGCGGAGAGGCGGTGGAGGAGGAGGATATTTAGCGGTG AGTTCTCCGGTTTCCGGCGATAAAGCTTACGCTAGCGAGAACGAAGTACAAAAGACGAATAACAATCAGAGGAGAAGGAG GAGATTGAAACCGGAGTGTCCACCGTGGGTTGATAGTATGCGGAGGAGCTACGTCGGAGATGAACAGAGTAGTCACGGTG GTTACGGAGGAGGAGTGGTGGTTGTTACGAGGCCTATAGGAGGAGGAAGGCCATTGTGTATGGATTTAGAAGAAGTCAAA GCTTGTAAAGATTTGGGGTTTGAGCTTGAACCGGGTCGGGTTTCGTATTCCGGGTCAACGGTGGATACTAGTAGTGGCGG CAATTCTCCTATCTCTTCTAACCACCGTATTTCGAGTCCCGGGGATGATCCAAAAGACGTGAAAGCGAGGCTTAAGGCGT GGGCTCAAGCTGTGGCTTTTGTGTCCACTACTCATCATCAACCTCCTAATTCTTTATGATTCACTTTCTTACAAAAAAAA ATTCACTTCAAAAAAAAAATAAAAAAAAATTGCATGCATTTTCGAGTTTCATTTTTGAAAAATTATTTTCGGGTTTTT ............................................................................i... ........................................N.......N............................... ................................................................................ .............................i.........i.......N.....i.......................... ....................................................N........................... ........................................i....................................... ................................................................................ ................................................................................ .....................................i.....................N.................... ...........................................................i.................... ................................................................................ ............................................N................................... .......................................................N........................ .................................N............................................ Pos Score Pred ------------------------ 77 0.618 Yes 121 0.198 - 129 0.425 - 270 0.677 Yes 280 0.665 Yes 288 0.481 - 294 0.701 Yes 373 0.447 - 441 0.842 Yes 678 0.568 Yes 700 0.365 - 780 0.542 Yes 925 0.197 - 1016 0.093 - 1074 0.068 -

Explain the output -- Processing locus 825: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATCTGTTTTTTTCCGACGATATTTCTTGCTCGATTCTGAAAAATCTAAAAATCCAAAATCGAAACCTGAAATAAAGGAA ATCGCAGACAAGTGGAGGTAGAAGACGACGAAGAAGATGTGGAGACGCGTGGCTTTACTTTCTCCGATGATTTCATCATC ATCGCGATCCCTTAAGCTCAGCCAGGCAGCTTCTGGGCTCAAAGTTGGGGAATCTTTTGCAACAGACATAATTAGTCAGG AAGAACGAGTTTCACCGCCGAAAGAGAAAGCTCCATTTATTACATTTGTACTAGGGGGACCTGGAAGTGGAAAAGGTACA CAATGCGAAAAGATTGTTGAGACTTTTGGATTACAACATCTAAGTGCTGGAGATTTGCTCAGGAGAGAGATCGCAATGCA CACGGAAAATGGGGCTATGATTCTGAACTTGATTAAAGATGGGAAGATTGTTCCTTCAGAAGTTACAGTAAAATTAATAC AGAAAGAGCTGGAATCGAGTGACAATCGCAAGTTTCTCATTGATGGTTTTCCACGAACTGAGGAGAACCGTGTTGCATTT GAGCGCATAATAAGAGCAGACCCTGATGTAGTACTGTTCTTCGATTGCCCTGAAGAAGAGATGGTGAAACGCGTCTTGAA TCGTAATCAGGGCCGAATTGATGATAATATAACTACAATGAAAAAGCGTCTGAAAATCTTCAATGCCTTAAATCGTCCTG TTATTGACTACTACAAAAATAAAGGAAAACTCTACACCATTAATGCAGTAGGAACAGTAGACGACATATTCCAACATGTC CTACCTATTTTCAATTCATTTGAGCAATTGAAGGAGAGTAGTCATGTAAATCCACAGTCCCATCTCGGTTCGAGTTTGGT AGAAAATTCATCTTGACAAAAGACCACCAGTCTGAAATTTGTTCATTACTCATGAATTTTGATGCACTATATGCAATTCG CATTGCATCTTTTTTTGTTTGTTGTACTTTGTAAG ................................................................................ ....................................N.............................N............. ................................................................................ ................................................................................ ..i........................................................................i.... ........N.......N.....................N......................................... ..........................................N..................................... .........................N..................................N................... ....................N................i........................N................. ..........................................N................................N.... ...........................................N.................................... ...................................................N.........N........N......... ................................... Pos Score Pred ------------------------ 117 0.470 - 147 0.283 - 323 0.517 Yes 396 0.558 Yes 409 0.332 - 417 0.234 - 439 0.071 - 523 0.269 - 586 0.228 - 621 0.375 - 661 0.149 - 678 0.546 Yes 703 0.471 - 763 0.204 - 796 0.373 - 844 0.466 - 932 0.062 - 942 0.046 - 951 0.064 -

Explain the output -- Processing locus 826: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAGGAGTAGTAAGAGGGTGAAATGGTTATTTCAAGTGTGAAGCGTGGGGACGTGGGTGACGGTGGTGATCTGAAACAGT GGTCTGCCGTTAAAACATTAAATTAAAACACGTGTTTTAAATGTCTCTATGTTAATTAATACTATAAGAGTATATTTTAC TAATTTCCGTATCTGCAACCAGCGAAAAAAAAGTAATAAAGAGGTCCTCCATTTCTTCCTCGTGACAAAACGCACTTGGC AGAGAAAGATAAACAAGAACCCTAAGTTTTTTTATAAGATTCGAGAAAATTCAACAACTCAGGAAGGAAGATCCTTTTGC TCCAATTTCTCAATCGAAACGATTTCAATTTCGGTTTCAACGATGTCGAGTTTTTCTGCCGAGTGTTGGGACTTCTCTGG TCGTAAACCGAGCTTTTCACAAACATGTACTCGATTGAGTCGTTACCTGAAGGAGAAGGGTAGTTTTGGAGATCTGAGCT TAGGGATGACATGCAAGCCCGACGTTAATGGAGGTTCACGTCAGCCTACAATGATGAATCTGTTCCCTTGTGAAGCTTCA GGAATGGATTCTTCTGCTGGTCAAGAAGACATTAAACCGAAGACTATGTTTCCGAGACAATCAAGCTTTTCTTCTTCCTC TTCCTCTGGGACCAAAGAAGATGTACAGATGATCAAAGAGACTACTAAATCTGTGAAGCCAGAGTCTCAATCTGCTCCGT TGACTATATTCTACGGTGGTCGAGTTATGGTGTTTGATGATTTTTCTGCTGAGAAAGCTAAAGAAGTCATTGATTTGGCT AACAAAGGAAGTGCCAAAAGCTTCACATGTTTCACAGCTGAAGTAAACAATAACCATAGTGCTTATTCTCAAAAAGAGAT TGCTTCTAGCCCAAATCCTGTTTGTAGTCCTGCAAAAACCGCAGCACAAGAGCCAATTCAGCCTAACCCGGCCTCTTTAG CCTGCGAACTCCCGATTGCAAGAAGAGCTTCACTTCATCGGTTCCTTGAGAAGAGGAAGGATAGGATCACATCAAAGGCA CCATACCAAATAGACGGTTCAGCTGAAGCGTCTTCCAAGCCTACTAACCCAGCTTGGCTCAGTTCACGGTAAACTTCGAG CCTGTCCGACCCAGAAGGCACAACTTGAGAGACCTTCTTGTAAGATTCTTCTGATGCTCCATCGTTACAAATATCAAGCT GCTCCTCTGTT ......................i......................................................... ........................................N.......N............................... ................................................................................ ................................................................................ ..........................................N..................................... ........................i....................................................... .....N....N................N......................N..N.......................... ...N.........................................i.................................. ....................N.......i................................................... ..........................i.........N........................................... ..........................i..................................................... ................................................................................ ................................................................................ ................................................................................ .....................................................N.......................... ........... Pos Score Pred ------------------------ 23 0.759 Yes 121 0.307 - 129 0.164 - 363 0.289 - 425 0.537 Yes 486 0.371 - 491 0.196 - 508 0.231 - 531 0.480 - 534 0.495 - 564 0.418 - 606 0.573 Yes 661 0.253 - 669 0.613 Yes 747 0.752 Yes 757 0.191 - 827 0.517 Yes 1174 0.043 -

Explain the output -- Processing locus 827: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGCTTTTGACTCATAGAAATAGAATCCAATATTCATCTCTTTTAAACTGACATTAAAGCCCTTCGCCTCCTTAAACCCA CATGCTAAATTCATTGCCTAACCACCCAAAACATCCAAAGGGTATTTTCGTCACTCCAAAAAATGTATGCATATCGAAAA ATACTTTAGCTTATATACACACACGTCGCATTGGCACACCTTTCACACCAAAAGCTACACAATGGCTAAAACCTCTCTTC CTCTCGCCGCCTCCTTCCTCCTCCTCATCTCCTTCTCCTCCGCCGTAGACACTTCCCGTCTCTTCCTCACCGTCGTCAAC AACTGTCCCTTCACTGTCTGGCCAGCCATTCAACCCAACGCCGGCCACCCCGTCCTAGAGAAAGGTGGCTTCGCTCTCCC AACTTTCACTCACCGCTCCTTCAACGTTCCAACCACACACTGGTCCGGTCGCATCTGGGCCAGAACCTGGTGTGCCCACT ACAACGGAAAATTCTCCTGCCTCACCGGAGACTGCGGAAACCGCCTGGAATGCAACGGTCTCGGCGGGGCACCACCAGCT TCTCTAGCTCAGTTCGACCTCCACCACGGTGGTCACCACGACTTCTCCTCTTACGGTGTCTCTCTCGTTGACGGTTACAA TGTTCCGATGACCGTGACTCCTCACGAAGGCCATGGTGTCTGTCCTGTCGTTGGTTGTCGTGAAGATCTAATAAAAACGT GTCCGGCTCATCTTCAGGTCCGGTCACACAGTGGACACGTGGTGGCTTGTAAGAGTGGGTGTGAGGCTTTCCATACAGAC GAGCTGTGTTGTCGTGGTCATTACAATAGCCCTAACACGTGTAAAGCTTCGAGCCATTCGCTGTTCTTTAAGCATGCGTG TCCTTCGAGTTTCACTTTTGCTCATGATAGTCCTTCGCTTATGCATGACTGTGCTTCTCCTAGAGAGCTCAAAGTCATCT TCTGCCACTAATGTTTCGGCCATGTTCTAGGGTTATTGTCGTAACATCGGACTGATCTTCTAATGCTTTTGTTTGGTTAG TCTGATTTTGATTGGTTTAGTATGCGATTT ................................................................................ .............................................................i.................. ................................................................................ ................................................................................ ................................................................................ .................................................N.............................. ...............................................................................N .......i........................N............................................... ................................................................................ .........................................................................N...... .......................N................N...N................................... ..........N..........N........................................N................. .....................N........ Pos Score Pred ------------------------ 82 0.456 - 143 0.628 Yes 147 0.549 Yes 222 0.846 Yes 530 0.253 - 640 0.405 - 648 0.851 Yes 673 0.438 - 874 0.359 - 904 0.135 - 921 0.266 - 925 0.244 - 971 0.294 - 982 0.120 - 1023 0.028 - 1062 0.045 -

Explain the output -- Processing locus 828: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAAACTTCCTTCTATTTCCTTTTCTCTAAAGTTTCTAGTACGTTCTATATAATATCCAATACCTCTCTAACTTCCTATT TTGTGATATCCAAAGTTCTGATTTTTTAACCCTAACGATCAACACTTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA GATGGTGATGAAGATTGCGATAGCAACAGAGAGAACAGATCTTCCTCGTTTGGTATCTACTGCAATTAGAGCTTCTAATT CGCAGAATGCTTCTGATGTTGAACGTTGCTTTGATGTTCTTCGATACTTGAAGGGTTTGAATCTCTCTGTCAAAAATCTT TCCCATTCCAAAGTGATACTTCCCTTGGAGAGTCTGAGAGATCACGAAAACCCTAAGATACGAACGGAAGCTCACGTCTT GTTCACTTCATGGATGAAGACTTTTTACTCCAGCGGACAAAACTCTTCTTCTACTTGCAACAAACCTAATCCCCTGAAAC TGAAGAAAGTTGTTAAAGCGTGTTCGGAGTTGAAGAAGAAGAACGAAGAGCAACTATCTCATGGTTTTGCGGTACTTAAG GCTAAGAAAGAGACTGTTTTTTTGGGGATGAAGAAGAACGAAGATGAGAGATCTAGGGTTCATGAAACCAGAGAGATGAA ACAAATTGGTGATTCCAAGTCTTTTGCATTGATGAGGACTATCGAGAAGAAGAAGCAATCGGCCTCACCAATTCAAGGTT CTAAAAACCCTAGATCCGGGGCGGGTGAAACGGGAGTGATTAAGAAAATTTTGAGGAAGCCTGACTTTTGCTGCAAGTCT GTTCCATCAAGGCCTCCTCTCAACATGAAGAAGCATCAACCGGTGAAAGCATTTGAAAACCCTAAAACTTGTCTTGTGTT GAAGAAGAACTCACCGGAGATGTTGGAGCTATTCGAGATGGCCAAGAAGTCAGCAGATGTTGCCAATGCCAAGGGATTTC TCGCAGCTAAAGAAGAGGCATCAATATGCGTCGACATACTCGCTTTACTCATGAAATTTTCCATCATCTCAACGGCTATT GAAACAAGACGGATCATGGAAAAGCTTGAGCGTCTCACAAAGCACAAAGATAGAAAGATATGCAATGCTGCATTAGCACT TCTCCACCATTGGAGGCAGACCATTAGAAATCAACAACAATGAGTAGTGTCATCAGAGAATCTGCGGAAGACAAGGGATG GAAATTAGTAGGATCGTGACTTGAAAGTTTTGGTTTTCTATTCGTTTAGTAAACTAGAGTAG ................................................................................ ................................................................................ ......N........N.................N.............................................. ................................................................................ .........N...N.................................................................. ............................................................N................... ...........................i...............N.................N.............N.... ...............................N................................................ ................................................................................ ........................i....................................................... ...................i.................N..................N........N.............. .........................i........................N............................. ...............N...........................................i....i............... .......................................N.....................................N.. .............................................................. Pos Score Pred ------------------------ 162 0.584 Yes 168 0.346 - 247 0.327 - 256 0.100 - 274 0.215 - 410 0.250 - 414 0.491 - 541 0.222 - 588 0.520 Yes 604 0.262 - 622 0.168 - 636 0.359 - 672 0.312 - 825 0.665 Yes 900 0.806 Yes 918 0.312 - 937 0.359 - 946 0.189 - 986 0.779 Yes 1011 0.365 - 1056 0.491 - 1100 0.665 Yes 1105 0.607 Yes 1160 0.370 - 1198 0.092 -

Explain the output -- Processing locus 829: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAAAACATGTAATGTTGAGCATCCCAAAGTTTGTGTGTCCCAATGCTAGAGCATATTATAGTCTTACCTCTAGTGAGT TCATAAAGAAGTGTTGTATGATCATATATTTTGTTGAGTAAGAGAAATATTTCTTTCTTCCTCATGTGGTTGATTATTCA AAATTATACAAAAGAGGAAAATCACTTAAAAATATTCACGATACCGTTAAAAGATTACCAAAGAATGGGCCAAATCATAA TATTAATAAAGCCCGTTAATAACCGAATCGACCCACCCGACAAAACAACCCGAATCGTTTTAATATCCAACCCGGAGTCA GACGGAGCTCCAAAGATTTGGCTTGTCTCTGGCTTGAGAACAAAGAAAACCCCTTTCTTCGATCTCAGCTTAAATCACTC GACGAAGAAGAAATCAGAAAAGAGTCAGAGAAAAAAAAAATCAAGGAAGATGAGTAGCATTGGAACTGGGTACGATCTCT CCGTCACCACTTTCTCTCCCGATGGCCGTGTTTTCCAGATCGAGTACGCCGCTAAAGCTGTCGACAACAGTGGGACTGTT GTTGGAATCAAGTGCAAAGATGGGATTGTTATGGGAGTTGAGAAACTTATTGCATCAAAGATGATGCTACCAGGTTCCAA CAGGAGAATCCATTCTGTTCATCGTCATGCTGGCATGGCTGTTGCTGGGCTTGCAGCTGATGGGAGGCAAATTGTTGCGC GTGCTAAGTCTGAAGCACGAAGCTATGAGAGTGTTTATGGCGATGCTGTACCTGTGAAGGAACTTTCTGAGCGTGTTGCA AGTTATGTACATCTGTGTACCCTGTATTGGTGGCTCAGGCCTTTTGGCTGTGGGGTTATTCTTGGAGGTTATGATAGAGA TGGACCTCAATTGTACATGATTGAACCATCAGGCATATCATATAGATACTTTGGTGCGGCCATTGGCAAAGGAAAGCAAG CTGCCAAAACGGAAATTGAGAAATTAAATTTATCTGAGATGACATGCAAAGAAGGCGTTATCGAGGTGGCGAAAATCATC TACAAGCTACACGATGAAGCCAAGGACAAGGCTTTTGAATTGGAGATGAGCTGGATATGCGAGGAGTCAAAACGAGAGCA TCAGAAGGTCCCTGATGATCTTCTGGAAGAAGCAAAGACGGCAGCTAAAACTGCGCTCGAGGAGATGGATGCTGACTAAA ACTGAGGAGTGTGTTGAATTTAGATCCGTCTTATTGCGTCGTTTGATGTTTCTTATGTACTCTGAATTGAATTTTGTCTG GAGGGACAGTTGTTGCAG ........N....i..............................N................................... .................N.............................................N................ ................................................................i............... ................................................................................ ................................................................................ .................................................i.............................. .....................N.......................................................... ...................N..........i.............................i..i................ ..........................N.......i........................N.................... ........................N...........N.....N..................................... ....N.................................................................N........N ................i............................................................... ......................................i....N.................................... .............N...............................i..........N....................... ..............N.................................................N...N........... .............................................N........N......................... .................. Pos Score Pred ------------------------ 9 0.246 - 14 0.652 Yes 45 0.330 - 98 0.240 - 144 0.095 - 225 0.748 Yes 450 0.808 Yes 502 0.227 - 580 0.326 - 591 0.710 Yes 621 0.752 Yes 624 0.582 Yes 667 0.202 - 675 0.837 Yes 700 0.200 - 745 0.304 - 757 0.394 - 763 0.147 - 805 0.265 - 871 0.405 - 880 0.198 - 897 0.600 Yes 999 0.634 Yes 1004 0.437 - 1054 0.449 - 1086 0.720 Yes 1097 0.493 - 1135 0.085 - 1185 0.438 - 1189 0.121 - 1246 0.053 - 1255 0.072 -

Explain the output -- Processing locus 830: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGATCTATTTCTTGGTTATGTATTCTTGATCAGGAAAAGGGATTTGATCATCAAGATTAGCCTTCTCTCTCTCTCTCT AGATATCTTTCTTGAATTTAGAAATCTTTATTTAATTATTTGGTGATGTCATATATAGGATCAATGGAGGAAGGTGGGAG TAGTCACGACGCAGAGAGTAGCAAGAAACTAGGGAGAGGGAAAATAGAGATAAAGAGGATAGAGAACACAACAAATCGTC AAGTTACTTTCTGCAAACGACGCAATGGTCTTCTCAAGAAAGCTTATGAACTCTCTGTCTTGTGTGATGCCGAAGTTGCC CTCGTCATCTTCTCCACTCGTGGCCGTCTCTATGAGTACGCCAACAACAGTGTGAGGGGTACAATTGAAAGGTACAAGAA AGCTTGTTCCGATGCCGTCAACCCTCCTTCCGTCACCGAAGCTAATACTCAGTACTATCAGCAAGAAGCCTCTAAGCTTC GGAGGCAGATTCGAGATATTCAGAATTCAAATAGGCATATTGTTGGGGAATCACTTGGTTCCTTGAACTTCAAGGAACTC AAAAACCTAGAAGGACGTCTTGAAAAAGGAATCAGCCGTGTCCGCTCCAAAAAGAATGAGCTGTTAGTGGCAGAGATAGA GTATATGCAGAAGAGGGAAATGGAGTTGCAACACAATAACATGTACCTGCGAGCAAAGATAGCCGAAGGCGCCAGATTGA ATCCGGACCAGCAGGAATCGAGTGTGATACAAGGGACGACAGTTTACGAATCCGGTGTATCTTCTCATGACCAGTCGCAG CATTATAATCGGAACTATATTCCGGTGAACCTTCTTGAACCGAATCAGCAATTCTCCGGCCAAGACCAACCTCCTCTTCA ACTTGTGTAACTCAAAACATGATAACTTGTTTCTTCTCCTCATAACGATTAAGAGAGAGACGAGAGAGTTCATTTTATAT TTATAACGCGACTGTGTATTCATAGTTTA ...................N............................................................ .............................................i.................i................ ................................................................................ ........................N....................N....................N............. ...............................N................................................ ...........N.................................................................... ................................................................................ .......................................................N........................ ....N..............i....................N....................................... ..................................................................N............. ................................................................................ ..................N............................................................. ............................. Pos Score Pred ------------------------ 20 0.107 - 126 0.737 Yes 144 0.674 Yes 265 0.345 - 286 0.316 - 307 0.262 - 352 0.288 - 412 0.481 - 616 0.366 - 645 0.297 - 660 0.769 Yes 681 0.321 - 787 0.289 - 899 0.127 -

Explain the output -- Processing locus 831: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACCAAAAACCGGTTCTTTTAGACCAAACCGAAATTGTCTACTCCTAGTCCTCCTGCATATATGCCCCAACACATCTCT CAATCCTCTCATCGTCAATAATATCTTTATATGACAATGGTGAAGCAGATAAGAAGATACGCTCTATTTCAAGCCACGCC AGATTCTGAGTTCGTGAAGGAGATGTACGGAGGCTACTTCAACGTATTCGTGTCGGCTTTCGGAGACGAAGGAGAGCAAT GGGATCTTTTCCGTGTGATCGACGGCGAGTTTCCTCGCGACGAAGATCTTGAGAAGTATGAGGGATTCGTCATTAGTGGG AGTTTACATGACGCTTTCACAGAAGAGGATTGGATCATTGAGCTTTGCTCTGTTTGCAAAAAACTTGATGTGATGAAGAA GAAAATTCTTGGCATATGCTTTGGTCACCAGATCATATGTAGAGTAAGAGGTGGGAAAGTGGGAAGGGCTCGTAAAGGAC CAGACATAGGCCTCGGTAACATAACGATCGTTCAAGATGTGATCAAACCGGGTGATTACTTCGATCAAATCGAGTCATTG TCGATCATACAATGTCATCGAGACGAAGTACTTGAGCCTCCAGAGTCGGCTAGAGTCATAGGATTCTCAGACAAATGCGA CGTTGAGATATTCTCAGTGGAAGATCACTTGCTTTGCTTTCAAGGTCATCCCGAGTATAACAAAGAGATTCTCCTTGAGA TCATTGATCGTGTCCACAAGATCAAATTTGTTGAGGAGGAAATTTTGGAGAAAGCAAAGGATTCGATCAAGAAGTTTGAA CCAGACACGCAGCGTTTGCACATGCTTTGCAAGAATTTTCTGAAAGGACGAAGAACCCACTAATTGGTTTAGTTTCCACG TTCATGGTCTAAATAAATTCTACTACGTTTGTGTAATTTCATAACTATTATATGGTCATGGTCATCACATAATGTAACAT AT ..............................................................N................. ..............................i.....i........................................... ......................i.......................................................N. .........................................................N...................... .......N...........................................................N....i....... ...............N....................N........................................... ....................................N........................................... ...........i..............................................................N..... ................................................................................ ................................................................................ .....................N.......................................................... ...N...............................................N.....N.............N........ .. Pos Score Pred ------------------------ 63 0.405 - 111 0.712 Yes 117 0.877 Yes 183 0.689 Yes 239 0.361 - 298 0.120 - 328 0.134 - 388 0.411 - 393 0.585 Yes 416 0.270 - 437 0.201 - 517 0.429 - 572 0.676 Yes 635 0.343 - 822 0.100 - 884 0.052 - 932 0.046 - 938 0.041 - 952 0.067 -

Explain the output -- Processing locus 832: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAACCCAGCCCCAAAAAATAACAGTATAGGTAATGATATTATGTCGCTTTAAGTAGATAATTACAACGTTTGAATAATCT GCATATTCAAAATTTCAATAGAGTTGGAACCAACATCGAAGCAAACTGTTGGAAAACAAGACTAACCGTCGTGTACGTAT CTCTTCGTCTATATATATGTATGTGTGCATAAATTTTAAAAACATGTACTCGTACAAAAAAATAAGTTACCAGATGGAAG AGGTAATGTCAATGATCTTCCACGGAATGAAACTAGTTAAATCGCTCGAGTCCAGCTTACCGGAAAAGCCACCGGAATCT CTATTGACATCTCTTGACGAGATCGTAAAGACATTCAGTGATGCAAACGAGCGGCTGAAGATGTTACTAGAGATTAAGAA CTCCGAGACTGCGTTAAACAAAACCAAACCGGTGATTGTGTCCGTTGCAAACCAGATGTTGATGCAGATGGAACCGGGTC TGATGCAAGAGTATTGGTTAAGGTATGGCGGGTCCACGTCGTCTCAAGGAACGGAGGCCATGTTCCAGACGCAGCTCATG GCCGTTGATGGTGGTGGAGAAAGGAATTTGACGGCTGCAGTAGAAAGATCCGGCGCTAGCGGTTCCTCCACGCCAAGGCA GCGGAGAAGGGCGTACTATAGGTGCACCCACCAAAAGCTATACAATTGCCCGGCCAAGAAACAAGTCCAACGCCTTAACG ACGATCCCTTCACCTTCCGGGTCACATACCGTGGCTCACACACTTGCTACAACTCAACCGCTCCAACCGCATCCTCTGCC ACTCCAAGTACCATTCCAATCTCATCCGTCACCACTGGTCATTCTGTTGACTACGGTCTTGCTGTCGTTGACATGGCTGA TGTTATGTTTGGTAGCGGCGGGGTCGGAACCAACATGGATTTCATATTTCCTAAAAACGATCCATCTTAACATCATCACC GTTTCCGGCAGCTGGATGACGGATTGACGGTGACGAAAACAATCAAGATTCATCTACGTACAGAGGAAAAATATACAGTA TTTCTTAAG ................................N.......i....................................... ................................................................................ ................N...N......................N.............................N...... .....i.....N..............N..................................................... ........................................N...................i................... .......................................................i.....N.....N............ ..N.....................i..................................i.................i.. .......i........................................................................ ................................................................................ ................................................................................ ........................................................................i......N ....i.............................N............................................. ...............N................................................................ ......... Pos Score Pred ------------------------ 33 0.243 - 41 0.656 Yes 177 0.386 - 181 0.123 - 204 0.494 - 234 0.367 - 246 0.555 Yes 252 0.250 - 267 0.366 - 361 0.171 - 381 0.561 Yes 456 0.509 Yes 462 0.341 - 468 0.230 - 483 0.312 - 505 0.511 Yes 540 0.709 Yes 558 0.723 Yes 568 0.652 Yes 873 0.799 Yes 880 0.275 - 885 0.769 Yes 915 0.329 - 976 0.055 -

Explain the output -- Processing locus 833: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGCTCTTTAGATTCTTTTCCCATTTTGCCCTAAAGTTAACTGACGTGGCATGTGACTTTTTCTCTGAGATCATGGTTAC ATCATCTGACACGTGTAATAACCCAACTCCACGAGATTAACCAACGGTATGAGAAAATCGATTTCGATTTTAAATTGGGA TTATTATTATTTTCAGCTTTCTCTGGAAGCAACAATGGCGATTGCTCTCTCGTCGTCGTCGACGATCACGTCCATTACTC TGCAGCCGAAGCTGAAGACGATTCATGGATTAGGGACAGTACTTCCTGGTTATTCGGTCAAATCTCACTTTCGTAGTGTC TCTCTCCGGCGTAGCGCCGTGGTCGTGTCGGCCATTACCGGAGCTTCTTCCGGAGCTGGAATAGGGAAAGGTACAGCTGA TTCGCTAGATACAGTGAAAGTCTTGGATTTGAGAGGAAATGAGATTCCGATTTCTGATTTATGGAAAGATAGGAAGGCCG TTGTTGCATTTGCTCGTCATTTTGGATGTGTGCTCTGTCGGAAACGAGCAGCTTATCTTGCAGAAAAGAAGGATGTGATG GATGCATCTGGTGTTGCTCTTGTTCTGATCGGACCGGGAAGCATCGATCAGGCTAATACTTTTGTGGAACAGACTAAGTT TAAAGGAGAGGTCTATGCGGATCCAAACCACGCATCATACGAGGCGCTTGAGTTCGTTTCAGGGGTTTCTGTTACATTTA CACCCAAAGCTGCTATGAAGATACTAGAGTCTTACATGGAAGGATACCGCCAAGACTGGAAACTCTCGTTTATGAAAGAT ACAGTTGAAAGAGGCGGCTGGCAACAAGGCGGAATCTTAGTTGCTGGCCCTGGGAAAGATAACATCTCTTATATACGCAA GGACAAAGAAGCCGGTGATGACCCGCCTGTTGAAGAGATCCTTAAAGCGTGTTGTGCTTGAAGAGTTGAAAGAATCGCAA TCGAAAAATAGATATTTAAAAAACAGTAACGACACTGCTATGCCCGGATACTCAACTAAATCCATAAAACACCATTACCA N.................................................N.....................N....... ................................................i............................... ..................................i............................................. ........................i....................................................... ................................................................................ ......................................N.....................N................... .........................N..............................................N....i.. ..............N................................................................. ..............N....................N...................................i........ ................................................................................ .................N.............................................................. .......................................N........................................ Pos Score Pred ------------------------ 1 0.208 - 51 0.278 - 73 0.235 - 129 0.537 Yes 195 0.820 Yes 265 0.523 Yes 439 0.228 - 461 0.304 - 506 0.423 - 553 0.125 - 558 0.845 Yes 562 0.235 - 655 0.244 - 735 0.430 - 756 0.437 - 792 0.739 Yes 898 0.276 - 1000 0.071 -

Explain the output -- Processing locus 834: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAATATACTAAGATCTAAGTTTTATATCATTGTAATTATCACCAACGAACTGTTAAGACCAAATTTGATTACTCGAAAT GGTTTTGGGTTTAGGGTATGAAGAACTGATCAAAAGATTCAATTTTTTATGAAGTCAACTTTCATCTGCAAATTGAGCAA TCTCAGATTTCGATTGTGTTTCAGAATCATGCCTGACAAGAACATCGTAGAGGACGTTATCGGTGATTTGGTGGATAACT TCACGGAGACTGTTCAGAAGAACAAACATGGTTCCTCCTTCTTCGAACAAGAAGATTCCGTCAGTTCTCGTTTCAACCGT TTGTTTGATCGCCAGAAGCCAATTCATCATGTCTTAGGCGGTGGCAAATCTGCTGATGTGTTGTTATGGAGGAACAAGAA GATCTCTGCAAGTGTTTTGATGGGAGCTACCGCAATTTGGGTGCTTTTTGAATGGATCAATTTCCATTTCCTGTCTCTCG TTTGCTATGCCCTCTTGCTTGGTATGATTGCACAATTCGTTTGGTCTAACGCCTCTGGATTCTTAAACCGCTCACAGTCT AGAGTGCCTCGCCTAGTTCTTCCAAAGGATTTCTTTGCTGAAGTCGGTGTGGCCGTTGGAAAAGAAGTTAACCGCGGTTT GTTGTTTCTTCAGGACTTGGCTTGTAAAGGGAATTTGAAACAGTTCCTCATGGCTGTAATCGGACTATGGGTAGCTGCAA TGGTCGGGAGCTGTTGCAATTTCCTAACTGTTTTGTATATCGGGTTTGTTGGGGCACACACAATGCCGGTTCTGTATGAG AGATACGAAGACGAAGTGGATGGTTTTATGGATTCTATGATCATGAAGTTTCATAGTCATTACAAGAAGCTTGATACTGG GTTTCTCAGTAGAATCCCAAGTGGAAAGTTTGGATTAAAGAAGCGTGAGTGACTAAATCACTCAAGCATTTTCTTATTTT ACCTTGTTTGTTGTTAGAACAATATGGATTATGGTTTGCTAAATGAATGTTTTGAGAATATTATTCATTCA ..............................................................................N. .................N..............................N............................... ............................i................................................... ...........................i.................................................... ............................N..........................N.........i.............. ...................N...............................N............................ ......N................N........................................................ ................................................................................ .................................................i................i............i ..............................................................i............N.... ...................N.......N........N.....N..................................... ................................................................................ .......................N......N...........N...N........................ Pos Score Pred ------------------------ 79 0.283 - 98 0.340 - 129 0.157 - 189 0.702 Yes 268 0.518 Yes 349 0.177 - 376 0.143 - 386 0.567 Yes 420 0.335 - 452 0.416 - 487 0.289 - 504 0.304 - 690 0.755 Yes 707 0.556 Yes 720 0.793 Yes 783 0.715 Yes 796 0.122 - 820 0.238 - 828 0.358 - 837 0.199 - 843 0.249 - 984 0.122 - 991 0.070 - 1003 0.050 - 1007 0.056 -

Explain the output -- Processing locus 835: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 836: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATGATGATAGGCACACACATGACACATAAAATCATAATGAACAGTAGTATGATTAGCAATCCATATTTCTTGGATAAC ACTTCTTCACAGCTTTTTTGACAGGTCACTATAACACCTTTTTCAGTTCATTTTTCATTTTCAATCCTCACCCACCCAAA CTCTCCCTTCAAAGCAATGTCTCTCCTCTCTCTTTCTCAATTCAAACAAACTTTATTAAACCTAAAAGAAACATTTCCAA TCTCTAATGACTTAGTTGATAGAATCTCATTTAGTTACCTAGTAATAATCTTCACACTAGTAAGAGAATCCTACTCTTCA CCAAACTACATCTCTCTCTATATAACAAACCCCAAAACATCTCAACATACACACACAACAACTACAACAATGGCTACTTC AAGAACATTCATTTTCTCTAATCTCTTCATCTTCTTCCTCGTTATAGCCACTACTTATGGTCAGGCTCCAGCTCCAGGCC CTTCAGGTCCAACGAACATAACCGCAATCCTAGAAAAGGCTGGTCAATTCACATTGTTCATAAGACTTCTTAAAAGCACT CAAGCCTCAGACCAAATCAACACTCAGCTCAATTCTTCCTCGAGTAATGGCTTAACCGTGTTTGCCCCGACTGATAACGC CTTCAACAGCCTCAAATCCGGAACCTTAAACTCATTGTCTGACCAACAAAAAGTTCAGCTTGTTCAGTTCCATGTCTTAC CTACACTCATAACCATGCCTCAGTTTCAAACCGTTAGTAACCCTTTACGCACGCAAGCTGGAGATGGCCAAAACGGTAAA TTCCCTCTTAACATCACTAGCTCCGGTAACCAAGTTAACATCACCACTGGAGTTGTCAGCGCCACCGTGGCTAACTCTGT CTACAGCGATAAGCAGCTGGCCGTTTATCAGGTTGATCAAGTTTTGCTGCCATTAGCCATGTTTGGATCAAGCGTGGCTC CTGCTCCGGCCCCTGAGAAAGGCGGCTCTGTTTCAAAAGGCTCAGCTTCCGGTGGCGATGATGGAGGAGATTCTACTGAT TCATCTGATGCAGAGAGGACTGGATTCGGGTTTGGGATCAGAATCACTACCGTTGCAGCCATTGCTGCTTCTTCTTCTCT GTGGATATAACTCGAGAGAGAGCTTTTCTTGAGTCTCCACATTTGGAATTTTAAGAATAACGAGAGCTTTAAGTTTTTGT TGTACTTTGAGATTGTTTTATAGATGTGT ..N..N..............N.................N...........N............................. ................................................................................ ................N............................................................... ......N......................................................................... .....................................................................i.......... ........................................................i....................... ................................................................................ ..............................................i................................. .......................................................................N........ ..............i................................................N................ ................................................................................ ..........................................................i..................... .........................................................N..N................... .......N........................................................................ ................................................................................ .......................N..... Pos Score Pred ------------------------ 3 0.262 - 6 0.332 - 21 0.219 - 39 0.168 - 51 0.309 - 177 0.410 - 247 0.497 - 390 0.903 Yes 457 0.612 Yes 607 0.587 Yes 712 0.448 - 735 0.635 Yes 784 0.371 - 939 0.890 Yes 1018 0.097 - 1021 0.212 - 1048 0.104 - 1224 0.061 -

Explain the output -- Processing locus 837: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGGAAAACTTACGAATTTTGTTTTAAAAAACAGTTAAATAGGAATCGATTTGGCACGATTTGGTATGTCTTTGGTTTTG GTAATTTGGTATGGTTAGTAAAATCTCCGGACACTCTGGGAGGGTCAGAGGGTCTCAGAGATGGGAACACATTATATAAA CCGTTGGAGGAACACAGCCTCTCGCTTAGCTAACAACAACTCAACGAGTGACATGGACATCGCCCTCTTCTCACCGGCGT CGCTCTTTGCTGCAGACGGTGACTCCTCTGATGGAGAGACGACGGAGACGAGCCAGAACTTCGTTGAGAGAAATCATCAG TTTCCTGGAATTGAACTGCAAATTCGGGAATTTGGTTTCCACCAGCTGAATGCAAACTTGCTGTGGCCAGGTACATTTGC CTTTGCTGATTGGTTACTGCAGCATCGATATTTGATCGAAAGGCGGCGCTGTCTCGAGATAGGAAGTGGCACAGGGGCAC TAGCTATATTTCTTAAGAAAGAGTTTAATCTCGACATAACTACTTCAGATTACAATGATCAAGAGATTGAAGACAATATT GTTCATAACTGTATTGCAAACAAGATTATCCCTTCTCTGCCTCATATCAAGCATACATGGGGTGATGAATTTCCAATTTC GGAACCAGATTGGGATTTGATCATAGCCAGTGATATCCTTCTATATGTGAAACAGTACCCAAACTTGATTAAGTCTCTGA CTTTTCTTCTCAAAAAATACAAACCAACCAATGTCGTTAGTCCAGCAGAAGGTAAGCTAAATGGAGCAGACACAGAACTG CCTCGTCCTGTGTTCTTAATGAGCTGGAGACGAAGAATTGGGAAAGACGATGAGTCTCTATTCTTTACGGGCTGCGAAGA AGCTGGCCTGGAGGTGAAGCATCTCGGAAACCGAGTTTATTGTATCAAGCTTAGAGAAAGCAACCTCTGAAGAATAGGCT GACAAACTCATGAATCATCCACATGCTCGCCTCTGAAACTAAAGGTTCTGTTTGTTTCTCTCGCTGCTTATTTGGATTTG CTTTTTCTG .................................................................N.............. ..........i.................................................i................... ....................................................i........................... ..............................i................................................. .................................................N.............................. ................................................................................ ......................................................N......................... ........................................................N.......N............... ............................................N................................... ..............................i.............................i................... ..................N..............................N.............................. ................................................................................ .........N............N......................................................... ......... Pos Score Pred ------------------------ 66 0.383 - 91 0.521 Yes 141 0.710 Yes 213 0.791 Yes 271 0.574 Yes 370 0.468 - 535 0.275 - 617 0.493 - 625 0.067 - 685 0.189 - 751 0.792 Yes 781 0.591 Yes 819 0.352 - 850 0.494 - 970 0.057 - 983 0.096 -

Explain the output -- Processing locus 838: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTTTAATTGCCGTTTGCACTAGACCCAAAAAGAGATATACTGTAAAGAATAGTATACTACTACATTAATGATAATTGAA AATATATAAAGAAAAGAATAAACAACGAAAAGTCTCCACTCTCCATTACCACACACCACCTATACATATATGCCATCACA CTTCCTATTCTTCACCAAAAACAAGCTCAATATTCTCTTCTTTCATTTCACTCGATCAAGTTTGAGCTTAGCTTTTAAAA AAAGAGAGAAACAAATCATGAGGACAATGGTTGATTTAGGAAAGCAAAGAGATCATAACTATCACCACATGCAAATCATA ACCACTCCTACCTCCGTGGATTGTTCTCGCGAATTCCGTCACCGACGAACACTTCGTTCACTCATCGAATGCATGATTCC ATACTGTTGCACTTACCAACAACAACCATACCAAAACGACACCCTTTCAGTCTCTTCCACTTCATCCTCGTCTTCATCCT CAGACCACTCTTCCTCCTCCCAGAGTAGCAGTACCGTAAGTGGAACTTTCTTCGGACACCGTCGTGGCCGAGTCAGCTTC TGCCTTCAAGACGCCGCCGTGGGATCTTCACCGCTGCTCCTCCTCGAGCTAGCTGTTCCTACGGCAGCTTTAGCCAAAGA AATGGACGAGGAAGGTGTTCTACGCATAGCCCTCGAGTGTGACCGTCGTAGAAGCAGCAATAGTCGTTCGTCTTCGATCT TTGATGTTCCTGTTTGGTCTATGTTTTGTAACGGAAGGAAAATGGGATTTGCCGTGAGGAGAAAGGTGACGGAGAACGAC GCCGTTTTCTTGAGGATGATGCAGTCTGTGTCAGTCGGAGCCGGAGTGGTGCCGTCGGAAGAGGAGGATCAGATGTTGTA TTTGAGGGCTAGGTTTGAGCGAGTTACTGGTTCGAGTGACTCGGAATCGTTTCACATGATGAATCCGGGTGGTAGTTATG GACAAGAGCTTAGTATATTTCTTTTGCGATCGTGAAATTAAGTACGGTATACTATGTCATGTTCGTTTTTATTTTTATAA ATGTAAATCTTAAAGTAATATATATGCAATTTTTATCATTTCCACTAAAAATAT ....................................................................N........... .....................................................................N.......... ................................................................................ .................i........i.........................................N........... ....................................................................N...N....... ................................................................................ ................................................................................ ................................................................................ ...N................i....................i...................................... ...............N..N.....................................................N....... .......................................................N..N..................N.. .....................................................N....N..................... N......................N.............................. Pos Score Pred ------------------------ 69 0.168 - 150 0.378 - 258 0.510 Yes 267 0.507 Yes 309 0.219 - 389 0.324 - 393 0.251 - 642 0.771 Yes 724 0.059 - 741 0.548 Yes 762 0.848 Yes 816 0.392 - 819 0.287 - 873 0.469 - 936 0.152 - 939 0.150 - 958 0.259 - 1014 0.064 - 1019 0.087 - 1041 0.044 - 1064 0.049 -

Explain the output -- Processing locus 839: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAGTCTCTATTCTCTATCCTCCATCAAGGTTCTCTCTGTCTCTGCTAATGTGTTTGTCTCCTTGCTTTTTGAGTTTTTTT TTTTTGAGGTTTTTTTTTTAAACCTGTATGCAGAAGAAGTTAGAGACGAAAATGGAGAATCCGCAAGTGTGTGAATGTTA CCACGGAGATGTGGAGGAGGAGAAGAAAAAGAAACAGAACAATACGACGTCGCCGGTTCACGTCGTTCTTAAGATTGATT TTCACTGTGACGGCTGTATCGCTAGAATCGTTCGCTTATCTCGTCGTTTAGAAGGAGTTGAAACAGTGAGAGCAGATCCT GATTCGAACAAGCTGACTCTGATTGGGTTTATCATGGATCCAGTGAAGATTGCTGAGAAACTTCAGAAGAAGAGCAAGAA GAAAGTGGAGCTGATATCTCCTAAACCAAAGAAAGACACCAAAGAGAACAATGAGAAAAAGGCTAATGATAAGACTCAGA CTGTGGTTGCTGTGACCACAGTGGTGTTGAAAGTGAATTGCTCTTGTGATGGTTGCATCAAGAGAATTCAAAAGGCTGTC TCTACGACCAAGGGAGTGTATCAAGTCAAAATGGACAAAGAGAAGGAAACGGTAACAGTCATGGGAACAATGGACATCAA GTCTGTGACAGATAATTTGAAACGCAAGCTGAAGAAAACCGTCCAGGTCGTGCCTGAGAAGAAGAAGAAGAAGAAAGACA AGGACAATGCAGAGGTAAACACCAAAGTCGGGTCTCCTTGTCAACCTGGAAATGGATGCACTCATGGGATTGGGCCTTAC AGGTTCATGGAAGGCCCAATGACTGGGTTTTTCAGTGAGGAAGATCAGAGCTATTGCAGCGTTATGTGAGTTTCGAGGTG ACAAAGCATTTGGAGTTTATTACAAATATATATACTAGGAGGTAAATAAACTAATAGGAAACAGAAAAGAAAAAAGAAGA AGAAGTAA ...............................................N................................ ...........................i.......................i......................N..... ........N....................................................................... ................................................................................ .................................i.............................................. ..................................................N..............N.............. ................................................N............................... ..............................i.............................i........i.......... ................................................................................ ......i............................................N...N.......N................ ......N...........N............................................N................ ................................................................................ ........ Pos Score Pred ------------------------ 48 0.243 - 108 0.642 Yes 132 0.753 Yes 155 0.338 - 169 0.196 - 354 0.759 Yes 451 0.148 - 466 0.229 - 529 0.369 - 591 0.834 Yes 621 0.622 Yes 630 0.695 Yes 727 0.700 Yes 772 0.453 - 776 0.380 - 784 0.247 - 807 0.332 - 819 0.137 - 864 0.388 -

Explain the output -- Processing locus 840: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCCCCGAGAAAACCACCTCCCAGTGTAGATAACCAGAAGAAGATAAGCCAAGGTGGGTCTTCTCAATTTTACCTTTTTA GTCCAAAAAACATGAAATTGAAACATGGGTCTTGATTCATGTATGGGTTTCGTCTTTAAAGTTTACTGTTTTTTTTTTGG TATTTTGCAGATGGAATCTGGAGAATTTCCTGCATGGCTTGAGGTTCTGTTAAAAGACAAGTTTTTCAACGCTTGTTTGG ATCATGAAGATGATAAGAAGAACGAGAAGAACATATTATGTATCGATTGTTGTCTTACCATCTGTCCTCACTGTCTCTCT TCTCATACCTCTCATCGTCTTCTTCAGATAAGAAGATACGTGTATAGAGACGTTTTGAGGGTTGAAGATGGTTCTAAGCT AATGGATTGCTCTTTAATTCAGCCATACACAACGAACAGTTCAAAAGTTGTGTTCATCAATGAACGGCCTCAGTCTCGAC AATTTCGTGGTTCCGGTAACATTTGTATCACCTGTGACCGGAGTCTCCAGTCTCCTTACCTCTTCTGCTGTCTCTCCTGC AAGATTAGTGACGTCATAATGAGACAAAGAGGACTCTCAGGGTTTCTCCGCGTCTGCAACGTTCTTGATTTAACCGACGA AGTCACGACAACGACACCGAGTTCCACTCTCGAGCCCACCGGGTCGAACCGAACATCTTCCGAGTCAAGTGGTAACGAAG GAGAAGACATGTTCTGGTGTCAGGCGCTCGCTTGCACCGCCACTACTGAAATCGTTAGGAAGAAAAGAAGCAGCTTGTCG ACGACTTGCAGGAGAGTCACGGAGGTGGTTTCAACGACTAACACGGAAGCTCCGGTGAACTTTTTAAACCGTCGGAAGAA TCCGCCGCAACGCGCCCCGCTTTATTAACTCGTGACGTTTGGCGAAATTTGGTTGGTCCAAGTGGTAAGTAGAATGAATA CACGTAACTACTATAGTCTTATTTTATATTGTTAATCAATATCTCTA ................................................................................ ...........N............i.............N...N..................................... ..........i......................N.............................................. ...N.....N...........................N.......................................... ...................................................................N............ ................................................................................ ..................i............................................................. ................................................................................ ........i....................................................................... ................................................................................ .........................................................................N...... ............................................... Pos Score Pred ------------------------ 92 0.133 - 105 0.737 Yes 119 0.289 - 123 0.256 - 171 0.781 Yes 194 0.169 - 244 0.091 - 250 0.161 - 278 0.242 - 388 0.417 - 402 0.539 Yes 460 0.694 Yes 579 0.762 Yes 729 0.537 Yes 954 0.060 -

Explain the output -- Processing locus 841: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATCGAGCTTAGAGTAGACTACATAAACACCCTTTTTCTCCTACTTTTAATTATCATCCCATACCTTTTTATACTTTTG GTTTGTTGTTTTTGGCATTGATGGTATGGGCTAAAAGTAGAAAATGGGCCCAATAAAAGATATCAATACCGGTGGCCCAT TAGCGGGTTTATGTCCTATCTACCCAAGTCCCAAGGCGCTGCAGCCAAGAAATGCGGGACGATGGTGATGCTCGGTGTGC TCCAGCTTCCCTGCCGCCTCCATCACTCTCTCTTTTACTCCTCTTCCCAAATTGCTTCTTCTCGCCCTCAAGGTGTTGCT AAAGTGGTTCTGAAAAAAGGCAAGACACAGCTCTTCAAAGACGGAAGTCCAATGGTGTACAGTGGAGCTGTCGACAGGAT CATCGGAAAACCACCTCCTCAGACAGGAGATGTTGTCATTGTAGCTGATGGCACTGAGAATCCCATTGGTTGGGGTTTGT ATAACTCTGTTTCTATGTTCTGTGTTCGCCTTATGCAGCTTCAACACGAATCCACCAGAGATCCTTCTTGTGCATTGAAC ATTGAGAAGCTTCTCCAAACAAGAATCGCTGAAGCAGTTCAGTTGCGAAAGAGTTTGGCACTTCCCTCGGCTAATACTAA TGCTTATCGTCTTGTCAACAGTGAAGGAGATAGATTGTCTGGATTGATTGTGGATGTGTTTGGAGATATAGCTGTGGTAG CATCCTCTGCTGCTTGGCTTGAAAAGTACAGGATTGAAGTGGAGAGTTGCTTAAGATCAATTGACGGAATTAATCATATA AACTGGAGACCTTCTCTTGATGTTCTTAAAGAAGATGGTTTTGACATCTCAAGTCTGAAACAGACACAATCATCTACTCT CCCACAGAGATCAATGGTACGGTGTGTGGTGCCCTTAATCAGGTTGTGGAAAATGGGATCTCATACGCAATTTCGCTAGA GGGACAGAAGACAGGGTTCTACACGGATCAGCGCGAAAACCGTCACTTCATATCAACCATCTCTGCTGGTAAAAGAGTTC TTGATCTTTGCTGCTATA ................................................................................ ....................i....i.................i.................................... ..........N........................................i.........i.....N............ ................................................................................ ...................................................i............................ .............................N.................N................................ ..............i.................N............................................... ...............................................................................N .....................................................N.......................... ................................................................................ ...................N..............N............................................. .............N......................................N........................... ................................................................................ .................. Pos Score Pred ------------------------ 101 0.616 Yes 106 0.629 Yes 124 0.873 Yes 171 0.278 - 212 0.507 Yes 222 0.680 Yes 228 0.281 - 372 0.812 Yes 430 0.205 - 448 0.356 - 495 0.662 Yes 513 0.247 - 640 0.242 - 694 0.108 - 820 0.220 - 835 0.231 - 894 0.377 - 933 0.406 -

Explain the output -- Processing locus 842: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTAGTTTTCTCGTTTCATGCCGTAGACCGTACCTGTAAATGTTTCAGACTGCGATGTTGTATTTTTCTTGACAATTGAGC TTCTGGGTAGTGTACTTTTGACTAGGCTCTGTCAAATGTGATAACTTTTTATTGATTGAATCGTCTGTTGTTTTGACCTT TTATATATCAAATGTGATTATGCAGGCAAGAGGATTGAAGAAGCATCTAAAGAGGCTCAATGCTCCTAAGCATTGGATGC TTGACAAACTTGGTGGTGCCTTCGCTCCCAAACCATCTTCTGGACCTCACAAGTCGAGGGAGTGTCTTCCTCTTGTCCTG ATCATCAGGAACAGGTTGAAGTATGCTTTGACTTACCGTGAAGTTATCTCTATCTTGATGCAAAGGCATATCCAAGTTGA TGGCAAAGTCAGGACTGACAAGACTTACCCTGCTGGTTTCATGGATGTTGTATCCATCCCCAAGACCAATGAGAACTTCC GTCTTCTCTACGACACCAAGGGACGTTTCCGTCTCCACTCCATCAAGGATGAGGAAGCAAAGTTCAAGCTTTGCAAGGTT AGATCTATCCAGTTTGGTCAGAAGGGAATTCCATACCTGAACACTTATGATGGTCGCACCATCCGTTACCCTGACCCGCT CATCAAGCCAAATGACACCATCAAGCTGGACCTTGAGGAGAACAAGATTGTTGAGTTCATCAAGTTTGACGTGGGTAACG TTGTGATGGTGACTGGAGGCAGAAACAGAGGGCGTGTGGGTGTGATTAAGAACCGTGAAAAGCATAAGGGAAGCTTTGAG ACAATCCACATTCAAGACTCAACAGGGCATGAGTTTGCCACAAGGTTGGGGAATGTGTACACAATCGGCAAAGGAACAAA GCCATGGGTGTCTCTTCCAAAGGGCAAAGGTATTAAGCTGACCATCATTGAGGAAGCCAGGAAGAGACTTGCTTCCCAGC AAGCTGCTTGAAAGTCCAACTGCTTTGAATCATCAAGACCCGTTTCTTTTACAGACTCGCTGCTTGTCTCAGTTTGTGAG TCTATTTCTTTTCTTATGTTGTTGGATTTC ................N.....................N..............N.......................... ...................................N............................................ ...........N.......i.......................................N................N... ................................................................................ ......................N..................................i.....................N ........................................N...N.......................N........... ................................................N............................... ..............................................N..N.............................. ...........N.................................................................... .....i.......................................................................... ............................N.......................N........................... ...N............................................................................ ................................................................................ ...............N.............. Pos Score Pred ------------------------ 17 0.344 - 39 0.220 - 54 0.201 - 116 0.262 - 172 0.242 - 180 0.612 Yes 220 0.242 - 237 0.354 - 343 0.312 - 378 0.674 Yes 400 0.284 - 441 0.493 - 445 0.219 - 469 0.220 - 529 0.313 - 607 0.146 - 610 0.356 - 652 0.368 - 726 0.783 Yes 829 0.216 - 853 0.219 - 884 0.333 - 1056 0.040 -

Explain the output -- Processing locus 843: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCAAGAAGAACATTCGGTTTGAGAGGAAGAAGAAGAAGAAGAGATTGATACTGAAACGACGGCGTTTTACGATTGTAAAT TACAAACAAAACCTTAACTGAGAACAACTTCGTTTTATCATGACACGACATCATTTTTTTTTTTTTTTGGTCAAAACACG ACATCGTTTAAGTTCCCACTTTTGTCTCTTCTTCGTTCTTTTAAGTTTTAATAGACGACGACGGCGACGAGAAGGAGAAT TAGCGAGATCGGAAGGAGAAAAAATGGATCAATTCTCAGGAGGAGGAGGTAATTGGTCGATGATCCCAAACGTGCAGGCG CAAGGTAACTTCGGTACACCGACGAATCATGACCAGCAACTATTCCTCCAGCAACAACAATTACAACAACAACAACAACA ACAACAACAACAACAGTTTCATCTTCAACAACAACAACAGACGCAGCAGCAACAACAACAATTCCAGCCACAACAACAAC AAGAGATGCAACAATATCAGCAATTCCAACAACAGCAACACTTCATCCAACAACAGCAATTCCAACAACAACAACGGTTG TTACAATCTCCTCCTCTTCAGCCACAGTCGTTACAATCACCTCCGCCGCAACAGACGATGGTTCACACGCCTCAATCTAT GATGCATACACCGCAGCAACAACAACAGTTGGTTCAAACTCCGGTGCAGACTCCTCAACAGCATCAGTCTTTAGCTTCTC ATTTCCATCTCTATCCGATGGTGGAGAAATTAGCTGATGTGATCGAAAATGGAACACGAGATCAGAATTCTGACGCCCTG GTGAATGAATTGAATAGCCATTTCGACAAGTGTCAACAGTTGCTGAATTCGATTTCAGGATCACTAGGATCTAAAACTAT GACTGTTGATGGCCAAAAGAGAAATGTTGAAGAAAGTGAGCAATTGCTTCAACAGAGAAGGGATTTGATTGTGGAGTATA GGAAATCTATTGAAGAGATTGTGACAATGGAGCATTAGTTTCTCATATTTTGATTCAGCAACTGTTTAGCTACTTAAGAC CAATAATTGTTATCAATAAACCAATCTTGATTCACATAATAAAGAGAATGTGGTTTT ................................................................................ .......................................N........................................ ................................................................................ .......................i...................................N.................... ............................N................................................... ................................................................................ .....N.......................................................................... .........................................................i....................N. .................i..................N...........i............................... ....N.........................................................................i. ........N..............N........................................................ ..........................N..................................................... ...............................................N......... Pos Score Pred ------------------------ 120 0.314 - 264 0.650 Yes 300 0.134 - 349 0.403 - 486 0.346 - 618 0.535 Yes 639 0.239 - 642 0.375 - 738 0.699 Yes 757 0.229 - 769 0.540 Yes 805 0.327 - 879 0.689 Yes 889 0.220 - 904 0.223 - 987 0.268 - 1088 0.048 -

Explain the output -- Processing locus 844: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACCAAAACTAAAGCTGAAAAGAAAATTTTAAGAAATAAGAAAAAGGAATAATAAACAAGAAGAGGACATGAAGCACAGA GATTCTGCAGACTGCAGGTGACCAATGGACACTTTATCAATAAAAACATACCTACTACTCTCTTACACTTTCAATTTTCC AATACAAATCCCAATCTTTAATCTCTCTTTCTTCTTCATCTCTCTTTCTCTTTCTCTCTTCATGGCTACAAGGATTCCAT TCACAGAATCACAATGGGAAGAACTTGAAAACCAAGCTCTTGTGTTCAAGTACTTAGCTGCAAATATGCCTGTTCCACCT CATCTTCTCTTCCTCATCAAAAGACCCTTTCTCTTCTCTTCTTCTTCTTCTTCATCTTCTTCTTCAAGCTTCTTCTCTCC CACTCTTTCTCCACACTTTGGGTGGAATGTGTATGAGATGGGAATGGGAAGAAAGATAGATGCAGAGCCAGGAAGATGTA GAAGAACTGATGGCAAGAAATGGAGATGCTCTAAAGAAGCTTACCCTGACTCTAAGTACTGTGAGAGACATATGCATAGA GGCAAGAACCGTTCTTCCTCAAGAAAGCCTCCTCCTACTCAATTCACTCCAAATCTCTTTCTCGACTCTTCTTCCAGAAG AAGAAGAAGTGGATACATGGATGATTTCTTCTCCATAGAACCTTCCGGGTCAATCAAAAGCTGCTCTGGCTCAGCAATGG AAGATAATGATGATGGCTCATGTAGAGGCATCAACAACGAGGAGAAGCAGCCGGATCGACATTGCTTCATCCTTGGTACT GACTTGAGGACACGTGAGAGGCCATTGATGTTAGAGGAGAAGCTGAAACAAAGAGATCATGATAATGAAGAAGAGCAAGG AAGCAAGAGGTTTTATAGGTTTCTTGATGAATGGCCTTCTTCTAAATCTTCTGTTTCTACTTCACTCTTCATTTGATCAT CTTTTGTTCTTATAACCTTGTATTTCTTGTTAAGATGGTAATGCAAATTCTCTCTTTTTTTGTCTAATTTAATTAGGAGT TTCTTTTTGTGATCC ....................................................................N........... ........................N....................................................... .............................................................i.................. .............i...................................................i.............. ................................................................................ ..........................i.....i....i.....i...............i...............N.... .........i.........i.....N.............................................i........ ................................................................................ ................i...i.......................................................i... ......N..N..N......i............................................................ ...........................N..............................N.....N............... ..........................N...N................................................. ..................................N.....N....................................... ............... Pos Score Pred ------------------------ 69 0.396 - 105 0.253 - 222 0.576 Yes 254 0.651 Yes 306 0.574 Yes 427 0.580 Yes 433 0.536 Yes 438 0.747 Yes 444 0.687 Yes 460 0.668 Yes 476 0.156 - 490 0.585 Yes 500 0.567 Yes 506 0.167 - 552 0.622 Yes 657 0.870 Yes 661 0.640 Yes 717 0.579 Yes 727 0.312 - 730 0.436 - 733 0.391 - 740 0.586 Yes 828 0.232 - 859 0.194 - 865 0.409 - 907 0.147 - 911 0.346 - 995 0.072 - 1001 0.092 -

Explain the output -- Processing locus 845: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAAGAAGAAGACGTTTGGTTGGGTGATAAAAGACTTGCAAACTCATAGGCGTTGCTCTGTTCCAATCCTAATTGGTGA CTCTTATTGGTAAAAGCATAGTTTCTTTTGTTATCTTTCAAATCTTTATCGTCAAAACGTGATCGTATATATCAAATCAT GAATGATCCCATATACCGTATTTGGTTATTCTTTCTTTCTTGCAGGCGTCTTGTTGCTCTTCCCAATGAAAACGGTTACT TTTCTCTGTTTCTCGAAGTTAATATGGAACTAATTCCATGTGGCTGGAGAAGATACGTGGAATTTCGCATGACTATAGTC AATCATATATCTCCGATACATTCTGTAGATAAAAAAGGATGGCGCTGGTTTGATGAGAACACTAAGAATTGGGGGTTTAA AGACATGATTCCCGCAGTCATCCTTAATAACGTAAATGTTGGGTTCTTGTTGAATGGGGAGATCACGATTATTGCTGAAG TTGAAGTTCATGAATTTATTGACACGTTAAATGCATCACAAGTTGAAGAGCTTAGTGATGATTCCTCTGAAGATTTGCAG AACAAAGATAATGTAACTATCGAAGTCAACGGCTTTCAGGTTCTTGATTCGCAGGTTGATCAGGTCAATGCAATCTTTGA GAAACATCCAGATCTCATATCGAATTTCAGTTTGAAGAATCAACATATCAGGAATGCTTACATGCATGCTCTTCTTGACC TTACGAAGACACTGTCCAAATCTACTAAGGAACTCACAGTGGAAGACATGAACGAAGCAGACAATACGATTACGGATCTG ATTAAAGCAGGCTTGAACTTGGACTGGTTGCGTCAGAAGTTTGATCAGGCTTTGGAAAAACAGATAGCTTACGACACACG GATCGGAGAACTCGAGAAACAGGTCAAGAAGCGAAAGCTGGCAGTGACAGAACTTGAAGCCGACCTGGAGAAAGAGAAAG CTGCAGCTTCGGCTTCATTGATGTTGTTTGACTGAGATTTTAAGATGAAGTTGTGATTTCTTGATTTGAGTCTTCTCCAT TGTTTAATGAGGAATAGTGTTTCCTACTTCTAGTCTTTTTATTTCAGCTTTCCT ................................................................................ ..............................................................................i. ..N..............................................................N.............. .......................N.............N..............................i........... ......................................i.............N........................... ....i..............................N.................N.......................... .........N....................N..........................N...................... ..........N........................................................N............ .....................................................N.......i...N.............. ...............................................i................................ ................................................................................ ................................................................................ ....................N.......................N................................... ......N............................................... Pos Score Pred ------------------------ 159 0.530 Yes 163 0.166 - 226 0.319 - 264 0.479 - 278 0.256 - 309 0.643 Yes 359 0.607 Yes 373 0.081 - 405 0.534 Yes 436 0.150 - 454 0.198 - 490 0.115 - 511 0.097 - 538 0.108 - 571 0.071 - 628 0.253 - 694 0.245 - 702 0.697 Yes 706 0.084 - 768 0.553 Yes 981 0.119 - 1005 0.073 - 1047 0.054 -

Explain the output -- Processing locus 846: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCTCATCTTAGTTTTTCAAATAAAATGCTATATTAGTAATTATGTTTAGTTAATTTGGTTACCACCAAATAGATCCATAA TTCCATATCATAAAATTATAACCACAGTGCGGATACGAACTACTCGTGTTAATTACTCCAAAAAAAAAGCAAAATCGCAA GAGAGACCTTAAACGACATCGTTTTCAGATGAGGAGACACCACCACCGATGCCAAATCGGCTCCGATAGCGAAACCGGAT CCGAATCCGATTCCGATACCGACACGCCGATGCGATGCATCTCTTGTCGAGAAGAGTACCTCCCTCGCAACGCCGGGACC TGCAAGGAATGCTACGTCGAAGCCGGCGAAACTGAAGAAGAACTCAAACGCGAGATCGATGACCTCAAAGCTAAAGTTGC TTTCCTTCGCTTATCTTCTTCTCTTGATCATGGAACTAGTTCAACCTCTAGATCCTTCACTGATGTTGTTCTCATCGCCT CTGAAGATAACGCCGGATCTCCTCCTATTCCCGCTCATAAATCCGTTCTGGTGAGTCGTTCTCCAGTTTTCAAAGCAATG CTTGAGAATGAAATGGAAGAGAGTCTAAGCGGCACGATCAAGATCAGCGACGTATCTTATGACGCTCTTCGGACTTTCGT ATATTATCTCTATACAGCTGAAGCATGTCTTGATGAGCAAATGGCATGTGATCTTTTGGTCATGTCAGAGAAATACCAAG TGAAACATCTCAAGAGTTATTGTGAGAGGTTTTTGGTTACCAAACTCAGTCCGGATAACTCTCTCATGACCTATGCCTTT GCACACCAGCACAACGCGAAACATGTTCTCGATGCTGCGTTGTCGCAGATCGTAGAAAATATGGATAAACTTACTAAAAG AGAGGAATATATGGAGCTCGTGGAGAAGGATCCTCGTCTTATCGTTGAAATTTATGAAGCCTATCTCTCGAAACAGGTTA ATACGGCAGCTGGAGGAACTAGTACGAGCAAAACTAGTTGAAATTTTCTTTATTGTCGACCTAAACAAGAGTAGATTCTG GTTTACCTTCATTTCGTTTTTAGGTATAGTATGGTTAGGGTCACCAAAACATGGGGTTTG ........................N................N...................................... ................................................................................ ............................i...................N............................... .............................N....N............................................. ........N.................................................N..................... .............................N................................N................. .............................................................................i.. .......N....i.............................................N..................... ........................N.......N.......i....N...............N.................. .................................................................i......N....... ......................N........N............................i................... ..........i..........................................N.......................... ................................................................................ ..............................N...................N......... Pos Score Pred ------------------------ 25 0.422 - 42 0.285 - 189 0.613 Yes 209 0.433 - 270 0.491 - 275 0.298 - 329 0.136 - 379 0.260 - 430 0.300 - 463 0.140 - 558 0.608 Yes 568 0.352 - 573 0.654 Yes 619 0.189 - 665 0.378 - 673 0.213 - 681 0.767 Yes 686 0.318 - 702 0.246 - 786 0.712 Yes 793 0.171 - 823 0.207 - 832 0.164 - 861 0.792 Yes 891 0.783 Yes 934 0.181 - 1071 0.070 - 1091 0.068 -

Explain the output -- Processing locus 847: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCTTAAGATTTGACCACAAGTCTAGTATATTGGATATTTGATTTTTGTAATGTTCTCTGATTAATTTCTCACATTTCCT TTTATGACTGTGCTCTGATGACATTTTATGTATGTTTGCATCGTGAACAGGGTCTTACCTCTCATGGAAGGGAACTTCTT CATCAGGTCTGATGCTCAACGAGCACATGACAATGGCTTCATAGCCAAACAAAAACCTAATCTCACCACGGCTCCAACAG CAGGTCAAGCTAATGAAAGTGGCTGTTTTGACTGCAACATCTGTTTAGACACAGCCCATGATCCGGTGGTCACTCTCTGC GGGCACCTTTTCTGCTGGCCTTGCATTTACAAGTGGTTACATGTTCAGTTATCTTCTGTCTCCGTTGATCAGCACCAGAA CAATTGCCCTGTTTGTAAATCCAACATTACTATCACCTCTTTGGTTCCTCTCTATGGAAGAGGCATGTCTTCGCCTTCTT CCACGTTTGGCTCCAAGAAACAAGACGCACTGTCCACTGACATACCCCGCAGACCTGCTCCATCAGCCTTACGCAATCCG ATTACCTCAGCATCATCTCTGAACCCAAGCTTGCAACATCAAACTCTGTCTCCTTCATTTCATAATCATCAGTATTCCCC TCGTGGCTTCACCACAACCGAATCAACCGACCTTGCCAATGCTGTAATGATGAGTTTCCTCTACCCTGTGATTGGAATGT TTGGAGACCTGGTCTACACCAGGATATTCGGGACCTTCACAAACACAATAGCTCAGCCTTACCAAAGCCAGAGGATGATG CAGCGTGAGAAGTCTCTTAATCGGGTATCGATATTCTTCCTTTGTTGCATCATCCTTTGCCTCCTTCTCTTCTAGCTCTC TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCGTGTATAGT ACGTGATGTACATA ..................................................N............................. ...N.............N.........i...N...............................i................ ...........N..............N.....i............................................... ............i............................................N...................... ........................................i....................................... .....................................................i..........i............... ................................................................................ ................................................................................ ......................................N.......N..i..........................N... ..........................................................................N..N.. ................................................................................ ................................................................................ .....N........ Pos Score Pred ------------------------ 51 0.454 - 84 0.350 - 98 0.346 - 108 0.736 Yes 112 0.427 - 144 0.614 Yes 172 0.489 - 187 0.308 - 193 0.715 Yes 253 0.506 Yes 298 0.244 - 361 0.505 Yes 454 0.557 Yes 465 0.735 Yes 679 0.322 - 687 0.468 - 690 0.792 Yes 717 0.499 - 795 0.153 - 798 0.257 - 966 0.045 -

Explain the output -- Processing locus 848: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATTGTTGAATAAAAATGAACTTTTAACCTTATTAAATTTTGCTCGGAGTCATTGTCCAAAAATACCAAGTTGGTTAAG AGTCCACAAGACCACAAGTAAAGTTTTCGTCAACTTTGTTTGACCAATGACCACACGGATAAATTGGTAACAAAAGGAAT TGTGGGTCCAAATTATAATAAACTGAAAATGGATGGTGTAAAATGTAATTTATCTACAAAATTAAACTTTAGGATAAACA TTGGATTTTTGCAGGAATCGCCGGAGGGAGAAGCAATGGTGACGATTCTATCAACTCCTCTCTCTCCAAGACTTACATTT CTCTGTGAAACAAAACTGTCATTATCTCGGAGCAATCGCTCGGTATGTTGTTCATTGAGTGAAGAACCAAAAGACCAATG CTTGAGTCGGAGAAGTCTTGTTTATGTTTTAGTTGCTTCGCCGTGTTTGTTGTTACCAGCGTTGTCTTCATCTGCGAAGA CTAAATCAAAGAGTCCTTATGATGAGAGACGGTTACTAGAACAGAACAAGAGGATACAGAGAGAGAACAATGCTCCTGAC GAGTTTCCTAACTTTGTTAGAGAAGGTTTTGAGGTTAAGGTATTAGCTTCTGATAATTACATCAAGGCTGATTCTGGCCT CATATATCGGGATTTCAATGTTGGTCAAGGTGATTTCCCAAAAGATGGTCAGCAGGTGACTTTTCACTACATTGGCTACA ATGAGTCTGGAAGGCGAATAGATAGTACTTACATACAAGGTTCTCCTGCTAGAATCCGCATGGGAACCAATGCACTTGTT CCAGGATTTGAAATGGGAATTCGTGACATGAAGCCCGGTGGGCGAAGAAGGATTATAATCCCTCCAGAATTGGGACCTCC GGTGGGACCTTCGACCTTCTTCAGCTCGAAGCAATTTGAAGTTTTCGACGTGGAGTTGCTCAGTATCCAGAATTGTGAGA GGAGGACTATAATAGGTTTCTACTCAGATGTCACATGCAGTTAACTTCCAAATGATGGTCATCACTAAGCTTCAATGAAC CTGCATTCTTCAATCTTCAATACTCTAAATCTGCACGGCAGATGAGAACTACAAAGAAACAAA ................i............................................................... ..............................................N................................. ............................i...N.........i..................................... ...................................i............................................ ............................................N................................i.. .......................N........................................................ ..................N..N...............................................N.......... ................................................................................ .................N..........................i................................... N..........................................................N.........N.......... ............i..............i.................................................... ................................................................................ ...........................i......N................N..N...................N..... .........................................N..................... Pos Score Pred ------------------------ 17 0.769 Yes 127 0.167 - 189 0.840 Yes 193 0.222 - 203 0.570 Yes 276 0.512 Yes 365 0.276 - 398 0.641 Yes 424 0.138 - 499 0.449 - 502 0.137 - 550 0.428 - 658 0.255 - 685 0.527 Yes 721 0.273 - 780 0.424 - 790 0.225 - 813 0.568 Yes 828 0.521 Yes 988 0.610 Yes 995 0.332 - 1012 0.316 - 1015 0.174 - 1035 0.136 - 1082 0.042 -

Explain the output -- Processing locus 849: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAAGAAATCATAAACTAATACTAAAACATTACACGCATCCTTAATCTACAATTAGTAAAACCTCTTTAGATTACCACCT TTTCATTCACCAAAAATATAACAAATTAATACTAAAGGCCCGGCCCATATGATTTGGCCCAGAAGAGACTTTAAGTTTCC TAATACAGTTAACGGTTTACTCAGTGAACCGGAGGGAGACAGCGAGATGAGGCCGGAGATAGTTCTGTTCGGCGACTCGA TCACGGCGCAGTCTTTTAGGTCCGGCGGTTGGGGATCTGCTCTTGCCGACGCTTACTCTCGCAAGGCTGATGTTGTGGTT CGAGGCTACGGCGGCTACAACACCCGATGGGCTCTCTTCTTGCTTCATCACATCTTCCCTCTCGGCTCTTCGTCTCCTCC TGTTGCTACGACGATATTCTTCGGTGCAAACGATGCAGCTCTCAAAGGAAGAACCAGTGATAGACAACATGTGCCGGTGG AAGAGTACACAGATAATGTCAGAAAGATTGTTCAGCATTTGAAGAAATGTTCACCTACAATGCTAATTGTGCTTATAACT CCACCACCAATTGATGAAGCTGGACGTCAAAGTTATGCAGAATCAATCTACGGTGAGAAAGCTATGAAAGAGCCTGAGAG AACAAACGAAACAACAGGGGTATATGCACAACATTGTGTTGCATTGGCCGAGGAACTCGGTCTGCGATGTGTCAACTTAT GGTCTAAGATGCAGGAAACCAATGATTGGCAGAAAAAGTACCTAAGTGATGGGCTCCATCTCACGCCTGAAGGCAATGGG GTAGTTTTTGATGAAGTCTCGAGAGTTTTTAGAGAAGCTTGGCTCTCTCCCGAAGAAATGCCGTTTGATTTCCCCCATCA TTCGCATATCGATGGTAAAAACCCATCGAAAGCTTTTGAAGAGCGTTGCTTATAACGATCATCCCCAAATTCATGAGCAG GTTTGTTTTGATTTAAATTCATGAACACGTTTCAATGTTGTGATTTAGAAAACTCTCGGATGTGAATAAATACC ................................................................................ ................................................N............................... ..............................................i................................. .....................................................................N.......... ..........................i..................................................... ................................N...................................N........... ...............N..............................i............i.................... .............N....................N............................N................ .......................N..........................................N...........N. ........i............N..........................i..........................N.... ..........N..............................................N...................... ...........N............................................................N....... ....................N.............N........................N.............. Pos Score Pred ------------------------ 129 0.357 - 207 0.781 Yes 310 0.235 - 347 0.625 Yes 433 0.427 - 469 0.284 - 496 0.295 - 527 0.769 Yes 540 0.503 Yes 574 0.200 - 595 0.443 - 624 0.408 - 664 0.281 - 707 0.179 - 719 0.426 - 729 0.569 Yes 742 0.425 - 769 0.508 Yes 796 0.136 - 811 0.243 - 858 0.475 - 892 0.375 - 953 0.046 - 981 0.035 - 995 0.093 - 1020 0.045 -

Explain the output -- Processing locus 850: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGCTGTTTGGTTTTAAACATTGTCGTTGATGTTCCTGCCTTGCTTCTCTGTGATTAACAATCTGTTCAATTTCTCAAAG GTGTCTTTTTTATTTGATTAGTATAGCTTATTTTTGGTTTGTTTATGGCATGCTATGTATGTAGGAGAAATGGCTGAATC CAAGGTAGTGGTTCCAGAGTCTGTGCTAAAGAAGATCAAGAGGCAAGAGGAATGGGCATTGGCCAAGAAAGATGAAGCTG TAGCTGCTAAGAAGAAGAGTGTTGAGGCCCGCAAGCTTATCTTCAAGAGAGCTGAGCAGTATGCCAAAGAATACGCTGAG AAGGATAACGAGTTGATCCGATTGAAGCGGGAGGCTAAGTTGAAAGGAGGTTTCTACGTTGACCCTGAGGCTAAGTTGCT CTTTATCATTCGTATCCGTGGTATCAATGCCATTGACCCAAAAACCAAGAAGATTCTGCAGCTCCTGCGTTTGAGACAGA TCTTCAATGGTGTGTTTCTTAAGGTGAACAAGGCAACAGTAAACATGCTGCGCCGAGTTGAACCATACGTGACTTACGGA TACCCAAACTTGAAGAGCGTTAAGGAACTGATCTACAAAAGAGGTTATGGAAAGCTGAACCACCAGAGGATAGCACTTAC TGACAACTCCATTGTGGATCAGGCTCTCGGAAAGCATGGGATCATCTGCGTTGAGGATCTCATCCACGAGATCATGACTG TTGGACCTCACTTCAAGGAAGCCAACAACTTCCTGTGGCCATTCCAATTGAAGGCACCACTCGGTGGCCTTAAGAAGAAG AGAAACCACTACGTCGAAGGTGGTGATGCTGGAAACAGGGAGAATTTCATCAACGAGCTTGTCAGGAGAATGAATTGATG AAAGCTTCCTTCTTTATCTTTGCAAATTTCAAAGATTTTGGGACCGAGTTTTTTTTTTTTTGTTCAAGTTATCAGAGACT TGTGATCTTTTATCCGA .............................N.................................................. ............................................N....N....i...N..........i.......... ...................................................i...................N........ ............................................................N................... ................................................................................ ..........................N..................................................... ......i.....................................i................................... ..............................................N................................. ...................................N.....................................N...... ................................................................................ .........................N...........................................N.......N.. ................................................................................ ................. Pos Score Pred ------------------------ 30 0.090 - 125 0.442 - 130 0.134 - 135 0.528 Yes 139 0.214 - 150 0.746 Yes 212 0.620 Yes 232 0.280 - 301 0.149 - 427 0.184 - 487 0.525 Yes 525 0.669 Yes 607 0.435 - 676 0.395 - 714 0.463 - 826 0.327 - 870 0.137 - 878 0.354 -

Explain the output -- Processing locus 851: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATGCATTTCAACAACTTGCATTCTCCACTGTATATATATGTGTGCGTCTATATTTACATTACGCATTGTGGAATCATAG TCCTTTTTCACCATAACATAAGCATATCAGAACACATTTTTTTACTCTGAAAATATGATAGTGACAATTGTTTCATACAC ACAAGAATTCACAATGGAAATCAGCGAACAAGAATCTGTGTTGGAGGTCAAGAAACGTCTAGGACAGTTTCTTCAGATAC CAACATCTTCTATAACTCTCTTTGTCTCTTGTTGGGAACTCATCGACGGTCTTGACATCGAAGATTACCCAATCATCTCT CACGGCACCAGAATCGACCTCACCGTAACTCCCCTTTTCACCGCACCGTCTTTTATCCACGCCGCCGTTAGGAAAATTCA CGTCACCGTCAAGTTCCCGTCAAAACAGTTCACCGTCGAAGTAGACAGAACCGAAACGGTAAGTAGCTTAAAGGACAAGA TTCACATTGTTGAGAACACACCGATAAAGCGAATGCAGTTATACTATTCTGGGATCGAGCTAGCTGATGATTATCGAAAC CTAAACGAATATGGAATCACAGAATTCTCAGAGATTGTGGTGTTTCTTAAGTCAATCAACCGTGCGAAAGACGTCGCTCC GGTAAGGAAACTCTGTTTCTTGGTGCAGACGTCATCGAGTTTATTCAACGGTGCGCGAATTCCAGTGGAGATTAAGGACA CTTGCACCATCTCGGAGATGCGAGAAGGGTTACAAGCTAACAAGACGTTGCCAAGAGACGAGTATATATTCGTACACAAG CAACGGATTATGCGAGAGAATTGTAGTCTTCGGTGGCATGGTGTTGAAAATGGTGACACTCTTTTTGTGTTTAAAGGGTC TATTAGTCGTGAAGGTTTCTGAGATTTTTAAAATTTAAGCTTCGTATTTGAATAAGTCCCTCTTAATAATAGTGATTAAT TAGTTGGATTATACATTCTTAAACATTGATGGAATATATAG ......................................................i......................... .............i.................................................................. ................................................................................ ................................................................................ ................................................................................ ................................N.................................N............. ..........N..................................................................... ................................................................................ .................N.............................................................. .........N...........................N...........N.............................. ................................................................................ ............................N............ Pos Score Pred ------------------------ 2 0.306 - 39 0.382 - 135 0.573 Yes 174 0.537 Yes 513 0.319 - 547 0.158 - 571 0.144 - 738 0.160 - 810 0.342 - 838 0.379 - 850 0.183 - 989 0.065 -

Explain the output -- Processing locus 852: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGATCTATTTCTTGGTTATGTATTCTTGATCAGGAAAAGGGATTTGATCATCAAGATTAGCCTTCTCTCTCTCTCTCT AGATATCTTTCTTGAATTTAGAAATCTTTATTTAATTATTTGGTGATGTCATATATAGGATCAATGGAGGAAGGTGGGAG TAGTCACGACGCAGAGAGTAGCAAGAAACTAGGGAGAGGGAAAATAGAGATAAAGAGGATAGAGAACACAACAAATCGTC AAGTTACTTTCTGCAAACGACGCAATGGTCTTCTCAAGAAAGCTTATGAACTCTCTGTCTTGTGTGATGCCGAAGTTGCC CTCGTCATCTTCTCCACTCGTGGCCGTCTCTATGAGTACGCCAACAACAGGTACAAGAAAGCTTGTTCCGATGCCGTCAA CCCTCCTTCCGTCACCGAAGCTAATACTCAGTACTATCAGCAAGAAGCCTCTAAGCTTCGGAGGCAGATTCGAGATATTC AGAATTCAAATAGGCATATTGTTGGGGAATCACTTGGTTCCTTGAACTTCAAGGAACTCAAAAACCTAGAAGGACGTCTT GAAAAAGGAATCAGCCGTGTCCGCTCCAAAAAGAATGAGCTGTTAGTGGCAGAGATAGAGTATATGCAGAAGAGGGAAAT GGAGTTGCAACACAATAACATGTACCTGCGAGCAAAGATAGCCGAAGGCGCCAGATTGAATCCGGACCAGCAGGAATCGA GTGTGATACAAGGGACGACAGTTTACGAATCCGGTGTATCTTCTCATGACCAGTCGCAGCATTATAATCGGAACTATATT CCGGTGAACCTTCTTGAACCGAATCAGCAATTCTCCGGCCAAGACCAACCTCCTCTTCAACTTGTGTAACTCAAAACATG ATAACTTGTTTCTTCTCCTCATAACGATTAAGAGAGAGACGAGAGAGTTCATTTTATATTTATAACGCGACTGTGTATTC ATAGTTTA ...................N............................................................ .............................................i.................i................ ................................................................................ ........................N....................N....................N............. ...............................N......................................N......... ................................................................................ ................................................................................ ..................................N............................N..............i. ...................N............................................................ .............................................N.................................. .............................................................................N.. ................................................................................ ........ Pos Score Pred ------------------------ 20 0.107 - 126 0.737 Yes 144 0.674 Yes 265 0.345 - 286 0.269 - 307 0.455 - 352 0.419 - 391 0.317 - 595 0.366 - 624 0.297 - 639 0.769 Yes 660 0.321 - 766 0.289 - 878 0.127 -

Explain the output -- Processing locus 853: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGAGAAAATAAATTAGGTTTCAGCATTCACTTTCTTTTTGACTATTTGAATTTTTTGTCTTGTGATAAAATTTGTTTTT GACTCTTTCACTCGTTGATAGGCCCGTTTAATGATAAGCAATAAGCCCAAAACTAAACTTATTTTGTGTTGGGTTTATTC ACATCTTTTAAGCCCATATAAGAAAGTTTTAAGATTTATAAAACGAATACGATATCGTTTCGACAATCGTCATTCTCCAG GACTCTTCTTCTCCTTTCCCAATTTGACGTTCTTTCATTTTTCCCGTTATCCAGACACACTCGCGAAAATGGCGGCTCAC TCTTCTTGCAACTTTGCTTTGACGAATCCGATCATCTCACAAATCGATTCTTTCTCTAAGAAGAAACTATCTGTTCCATT ATACTTCTTCTCTACAAGAAAAGCATTGACGAATCCTTGGTTAGGCGTTGTTGATTCATCCCTCTCTCTCACTTCTCCAG TCTCCGCTCTACAAACTAATCGCCCAAGAAGAATCCATAAATCCGCCATCTCCAGCTTACCCACCGCGAATCCAGATTTA GTGGTTTCTGATGCGAAAAAGCCCAAATGGTCATGGAGGGCAATTAAATCATTTGCAATGGGAGAGCTTGAAGCTAGGAA GCTCAAGTATCCAAATACTGGAACTGAAGCACTTCTCATGGGTATTTTGATTGAAGGAACTAGTTTTACTTCAAAGTTTT TGCGGGCGAATAAGATTATGCTTTACAAAGTGCGTGAAGAAACGGTCAAGTTACTAGGAAAGGCAGATATGTATTTCTTT AGCCCGGAACATCCTCCTTTAACTGAAGATGCTCAAAGAGCACTTGACTCTGCTCTGGATCAGAATCTAAAAGCAGGTGG TATCGGGGAAGTAATGCCAGCACATATACTACTTGGGATTTGGTCTGAAGTTGAATCTCCAGGTCACAAGATACTGGCAA CTCTTGGTTTCACTGATGAGAAATCTAAAGAGTTAGAGTCTTTTGCCTCTGAATCTGGCTTTTTAGATGAATAGTATATT ACATCCATATCATTTCTCCATTAATGCAAAATCTCCAAGTTCATAGTTGCTGTTTGCAATAATATTCGTATGTGTTCGTC TTTGCTCATTCTT ................................................................................ ..............................N................................................. ................................................................................ ....................................................................N........... ................................................................................ ................................................................................ ................................................................................ ..........i...............N.....i........................N...................... .....................................i.......................................... .................N..................................................i........... ............................N................................................... .............i.................................................................. ...............N..................................................N............. .......................N.............................................N.......... ............. Pos Score Pred ------------------------ 111 0.161 - 309 0.471 - 571 0.655 Yes 587 0.463 - 593 0.601 Yes 618 0.496 - 678 0.599 Yes 738 0.477 - 789 0.610 Yes 829 0.137 - 894 0.826 Yes 976 0.295 - 1027 0.231 - 1064 0.059 - 1110 0.035 -

Explain the output -- Processing locus 854: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGGAAAACTTACGAATTTTGTTTTAAAAAACAGTTAAATAGGAATCGATTTGGCACGATTTGGTATGTCTTTGGTTTTG GTAATTTGGTATGGTTAGTAAAATCTCCGGACACTCTGGGAGGGTCAGAGGGTCTCAGAGATGGGAACACATTATATAAA CCGTTGGAGGAACACAGCCTCTCGCTTAGCTAACAACAACTCAACGAGTGACATGGACATCGCCCTCTTCTCACCGGCGT CGCTCTTTGCTGCAGACGGTGACTCCTCTGATGGAGAGACGACGGAGACGAGCCAGAACTTCGTTGAGAGAAATCATCAG TTTCCTGGAATTGAACTGCAAATTCGGGAATTTGGTTTCCACCAGCTGAATGCAAACTTGCTGTGGCCAGGTACATTTGC CTTTGCTGATTGGTTACTGCAGCATCGATATTTGATCGAAAGGCGGCGCTGTCTCGAGATAGGAAGTGGCACAGGGGCAC TAGCTATATTTCTTAAGAAAGAGTTTAATCTCGACATAACTACTTCAGATTACAATGATCAAGAGATTGAAGACAATATT GTTCATAACTGTATTGCAAACAAGATTATCCCTTCTCTGCCTCATATCAAGCATACATGGGGTGATGAATTTCCAATTTC GGAACCAGATTGGGATTTGATCATAGCCAGTGATATCCTTCTATATGTGAAACAGTACCCAAACTTGATTAAGTCTCTGA CTTTTCTTCTCAAAAAATACAAACCAACCAATGTCGTTAGTCCAGCAGAAGGAGCAGACACAGAACTGCCTCGTCCTGTG TTCTTAATGAGCTGGAGACGAAGAATTGGGAAAGACGATGAGTCTCTATTCTTTACGGGCTGCGAAGAAGCTGGCCTGGA GGTGAAGCATCTCGGAAACCGAGTTTATTGTATCAAGCTTAGAGAAAGCAACCTCTGAAGAATAGGCTGACAAACTCATG AATCATCCACATGCTCGCCTCTGAAACTAAAGGTTCTGTTTGTTTCTCTCGCTGCTTATTTGGATTTGCTTTTTCTG .................................................................N.............. ..........i.................................................i................... ....................................................i........................... ..............................i................................................. .................................................N.............................. ................................................................................ ......................................................N......................... ........................................................N.......N............... ............................................N................................... ..............................i................................................. ......N..............................N.......................................... .............................................................................N.. ..........N.................................................................. Pos Score Pred ------------------------ 66 0.383 - 91 0.521 Yes 141 0.710 Yes 213 0.791 Yes 271 0.574 Yes 370 0.468 - 535 0.275 - 617 0.493 - 625 0.067 - 685 0.231 - 751 0.697 Yes 807 0.422 - 838 0.473 - 958 0.057 - 971 0.096 -

Explain the output -- Processing locus 855: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACGAGGCGAGGAAAGACGAAGAAGGAGATAGAGGATTAAATGGGCCATAAGGCCTTTCATGGGCCTAATATATGGGCTCA TCAATTTTAATTCCTCTCTAGGCGCTGCAATATAACAGAGTACGAATGAATTCTAGTGAGAGACAGAAAGTGAAATTCAA AAACAAAACAAAATCAGATCATCGCGCCATTCGCAGAAGCTCAGAGAAAAACTTCATTCGGAAGAAGAACTCAGTAATGG CGACGTATCCTTCTGTGATTGAGAGAACCGTTGCTTCGTCTCTTCTTCTCCTTTCCTATGGACCAGTGTTCTTCTCACCT AAAAGGTCGGAATCTGTAGAGGAATCGAGCTATGTGAGGAAGTGGTGTCACGAAGGAAGTTCGAACTTGAGCTTGATGTT GGGATCTAGCGGATCGAGATCTTGTGGCTCGGCTTTGTCCAGCGATGGTTCGTTTGGGATGAGTGAGGATCGCGAATTCA AAATCAATTATACTGGAGATCCGTTTCGTCTCATGAATTTCAAGACAGCGAGAAAACGCCGTTCTCAGGTCATTTGGGGA TCCTTCAACTTTAAGCCAACACATTTGAAGATGAATCCTGTGTGTGATGTTCTCTCAACATGCTCTGTTGATTCCAAAGA TGAGTCATGTTTGTCAACTGGTTCAAGCGAGGTTTCTAGCGTAGAAAGCAGAATCAAAGTCAGGAATCAGAAAAGCAATG AGAAACTTAGAGGAGGAGGGAAGACGATGAAAGAAAGTAGCAGGTCAAGTTCTATTCGCCGTAGAGCCAAAGACATCTTG GAGTTTCTTTCCTCTGAATCTTCTTCAGAAGTTCACATCCGTCAGATTCTTGGCGACAGTCCCGATACCAGCAAAGCTCT CAGAATGTTGTTGAAGATGGAGGAAGTGAAGAGGTTTGGCACAGGAGGAAGACTTGATCCCTTTATTTACAAGATTGCAT GAAAGATTAAGACATTAATGCTGGAGGCAGGTAACACTTATATCCCCTTTCCCACTATCTAGTTATATACAGTTTGGTTT GATCCTGATATGGGCTATAGG .......................................N..................N............N........ .............................................N.................................. ............................................................................i... .........................................................N...................... ...............................N...........................................N.... ............................................N.............N..................... ................................N............................................... ..............................i...............N............N...................i ......i......................................................................N.. ..........................N..................................................... ................................................................................ ....N...........N.............................................................i. .................N.............................................................. .........N........... Pos Score Pred ------------------------ 40 0.325 - 59 0.365 - 72 0.379 - 126 0.478 - 237 0.644 Yes 298 0.155 - 352 0.468 - 396 0.359 - 445 0.275 - 459 0.321 - 513 0.159 - 591 0.768 Yes 607 0.221 - 620 0.226 - 640 0.538 Yes 647 0.640 Yes 718 0.176 - 747 0.349 - 885 0.410 - 897 0.199 - 959 0.517 Yes 978 0.212 - 1050 0.062 -

Explain the output -- Processing locus 856: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACATAGATATATAGATTCCATCTCAAATTCGGTCTTATCATCTTTTTTCCTACTTCATCCCATCAAAGTCTCTCTAGCT ATATATTCTTCTCACACATAACATAAGTGGACAAAAAAAATGTGTACCTTAACGCCAATGTTTCCAAGTAAGCAGCAAGA ATGGTACTCTGCTTCAACAATGGAGTATCCATGGCTGCAGTCTCAAGTTCATTCCTTTTCACCTACTCTCCATTTTCCTT CCTTCCTTCATCCTTTAGATGATTCCAAGAGCCATAACATCAATCTTCATCATATGAGTCTTAGTCACAGCAATAATACT AACAGTAACAATAACAATTATCAAGAAGAAGATCGAGGAGCGGTGGTTTTGGAGAAGAAACTGAATCACAACGCAAGCGA ACGAGACCGCCGTAGAAAACTTAACGCCTTGTACTCTTCACTTCGTGCTCTCTTGCCTCTTTCTGATCAAAAGAGGAAGC TGAGCATTCCTATGACGGTAGCGAGAGTAGTGAAATACATACCAGAGCAGAAGCAAGAACTTCAACGTTTGTCTCGGAGA AAAGAAGAGCTCTTGAAGAGGATCTCGAGAAAAACTCACCAAGAGCAGCTGAGAAACAAAGCAATGATGGACTCAATAGA TTCTTCTTCCTCTCAACGGATCGCAGCAAATTGGCTCACTGACACAGAGATTGCTGTCCAGATTGCTACGTCGAAATGGA CATCTGTTTCAGACATGTTGCTTAGGTTAGAAGAAAACGGGCTTAATGTCATAAGCGTCTCTTCTTCCGTTTCTTCCACC GCAAGGATCTTCTACACTCTACATCTTCAGATGAGAGGAGATTGCAAAGTGAGACTGGAGGAACTCATCAATGGTATGCT CTTGGGATTACGCCAATCATAATGAGCGATATGAAGTGTTTAAAAGAGTGAATGTTGTTAGTTTATGTTAGACTTTTATC AAATCTGTAACATTTTTTTCTTCTTCTGATTAATGATTATT ................................................................................ .......................................i.................N...................... i..................N..........N................................................. ..................N..................................i.......................... ................................................................................ ................................................................................ ...........N.................................................................... ...............................................................i..i............. ...........................................................................i.... ..............N..............................N.................................. ..............................N.......................................N....N.... .....................N........N....................N............N............... ................................N........ Pos Score Pred ------------------------ 120 0.865 Yes 138 0.155 - 161 0.540 Yes 180 0.388 - 191 0.307 - 259 0.463 - 294 0.786 Yes 492 0.478 - 624 0.753 Yes 627 0.604 Yes 716 0.674 Yes 735 0.279 - 766 0.317 - 831 0.429 - 871 0.401 - 876 0.071 - 902 0.120 - 911 0.052 - 932 0.061 - 945 0.055 - 993 0.032 -

Explain the output -- Processing locus 857: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCGAGAACTGCCCCACGAAATTACACGTCTGAATAGATAAATCGAAAATGGATCGTGAGGCCCACGTGTCTGCGAAGAA GGGCCCACAGTGGATTATTGAAAAGAAGAAATGGAAAATCTGAGGATTGATTCGTCTCAGAGGCCGAGGTTGAAGATTAA TCGGACAAAGGAGATTAGAAGAGTAGAAAAAACGACAAGAAGAAAGAAACACAGTTGCAGGAGAGAAAGAGATATGGCTG TCCGGATAAACGCGATGGCGGTGACGTTTGTGGCGCACGCGCTGGCGGTAATAGCGGCGATTATGGTACTGGTTTGGAGT ATCAGTTACAGAGGTGGATTGGCCTGGGAAGCTACTAACAAGAATCTCATCTTCAATCTGCATCCTGTTCTGATGCTCAT CGGATTTATAATCTTGGGAGGAGAAGCCATCATAAGTTACAAATCGCTTCCGCTGGAGAAACCAGTGAAGAAGTTGATCC ACCTTATACTCCATGCCATTGCTCTGGCTCTTGGGATATTTGGCATCTGTGCAGCCTTTAAGAACCACAATGAAAGCCAT ATCCCTAATCTCTACAGTCTCCATTCCTGGATTGGTATTGGAGTCATTTCTCTTTATGGCTTCCAGTGGGTGTACAGCTT CATAGTGTTCTTCTTCCCAGGAGGATCAACAAATTTGAAAAGCGGATTGCTTCCGTGGCACGCAATGCTTGGCCTGTTTG TTTACATACTTGCTGTCGGGAATGCAGCTTTAGGGTTTCTGGAAAAGCTGACTTTCTTGGAGAATGGAGGGCTTGACAAG TATGGATCCGAAGCATTTCTTATCAACTTCACGGCCATTATCACTATTCTCTTTGGTGCCTTTGTGGTACTCACTGCTTC TGCTGAGTCTCCTTCTCCTTCTCCTTCCGTCTCCAATGATGATAGTGTTGACTTCAGTTATTCTGCTATATAAAACACTT TCTACATTCCACGTTACATCTGTATCGCCCCTTGTACTCTCAAAACTCCATTTATGTATGATGCATTTGTAGCGAAAAGC TTTGTGTGTTAC ................................................i............................... ..............................i................................................. .........................................................................i...... ..............i...............................................N................. ........................................................................N....... ................................................................................ ............N........................................................N.......... .......................................................i........................ ................................................................N............... .....................N.........................................N................ ...................................N..N......................................... .....................................................N...N..N................... ............ Pos Score Pred ------------------------ 49 0.624 Yes 111 0.725 Yes 234 0.774 Yes 255 0.729 Yes 303 0.287 - 393 0.309 - 493 0.158 - 550 0.327 - 616 0.519 Yes 705 0.457 - 742 0.095 - 784 0.347 - 802 0.210 - 916 0.105 - 919 0.238 - 1014 0.073 - 1018 0.057 - 1021 0.046 -

Explain the output -- Processing locus 858: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCAAGTGTTGGTTACATGTTAGTTACGCAGGCTCGGACCTGGTCTCAAAACATGGGCCTCAAATGGGCTGACCCAATAG ATTTGCTAAAACAAATATTAATCTAATCTAGACCATGTAGTCGTGGGCCATTTATTAGATTTGCGGAATGGGACAGACGA GATAACGCGGGAGAGAATCCAATCACCGATTGAGCTATGGGCCCCAGTATCGATTTCTACTATGACGTGGCACCACTTAA ATCACAATATCCGTATTCCCTCTCTCTCTCCCTTTCTATCTGAGCTTCTCTCTCTTACTCTCTCCAGAGCAACAATGGCT GCGACTGCTTCTTCTCTTACAATCGCGTCGTCATTCTCCGAACCACGGACTCAGATCCATTCCTCGAGACGTTTGAATCT CCCTCTTCAATATTCAATCCCGTACAAGGTGTTACGGAGTAGGAGTAGAAGATTAGGACTCGTCGTCAGTTCTGTTTCAG CTCCCAACGTTGAGCTCCGTACTGGACCTGATGATCTCATTTCTACCCTCCTCTCTAAGGTTGCGAATAGTGATGGAGGT GTGACGCTAAGCCCTGAGCAGCACAAGGAGGTGGCACAAGTGGCCGGAGAGCTTCAAAAGTACTGTGTCAAGGAGCCTGT CAAAAATCCTCTCATTTTTGGAGATTGGGAAGTGGTGTACTGTTCTAGACCAACCTCTCCTGGTGGAGGCTACAGAAGCG TGATAGGCCGTCTCTTCTTCAAAACGAAAGAGATGATACAAGCCATCGATGCTCCTGATATCGTTAGGAACAAAGTTTCC ATTAATGCTTTTGGTTTTCTAGACGGAGACGTCTCCTTGACAGGGAAGCTGAAAGCGTTGGACAGTGAGTGGGTGCAGGT GATATTTGAGCCTCCGGAAATCAAGGTTGGATCTTTGGAGTTCAAATACGGGTTCGAAAGCGAAGTGAAGCTTCGGATCA CATACGTTGATGAGAAACTTAGGTTGGGATTGGGATCTAAAGGATCATTGTTCGTCTTTAGAAGGCGTCAATAATATATA CGTTTCTCCCTCGTGCATCAACCAATGCAAATCACTACAGAAAAAAAGGAAACAACATATGGGGTCCTGCGAGAGTATGA TGGTCAATATCCA ................N...................................N..........N................ ..................................N................................N............ ....................................N........................i.................. ..........................................................................i..... ................................................................................ ................................................................................ ..............................N.........................................N....... ................................................................................ ................................................................................ ................................i...............N............................... ....N........................................................................... ................................................................................ .........N...................................................................... ........................N.................................N.................N..N ............. Pos Score Pred ------------------------ 17 0.424 - 53 0.447 - 64 0.493 - 115 0.279 - 148 0.408 - 197 0.373 - 222 0.510 Yes 315 0.851 Yes 511 0.164 - 553 0.440 - 753 0.723 Yes 769 0.223 - 805 0.240 - 970 0.137 - 1065 0.089 - 1099 0.106 - 1117 0.048 - 1120 0.048 -

Explain the output -- Processing locus 859: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGAGAAACCACGAATCTTACAAAAACAAAATCAAGCTGGTCCTGTCATGGTTACAAAATCCACAAAAAAAATAATTTCT TATATTGATATAAGCTAATAATTGGGAGAAAAGAGACAACGCCGTAGCAGAGGCGATGCTGGCTTTTTGGATATTACCAA ACTACTATTTACGTATTATCGTCGTCGCAAATACAAATACAAATACAAAAAAGGACTTGATTATTTGGGCCTAATGGCCC ATATATTTTTAAAAATATACCGGGCCCAGATTCACACTCACAGATACGTCGTCGTTTTTGTGTCGTTTGGGGTTGACTCT GAAGAATTTCTTTCCTCACAAGACACAAAAACAATGTTAAGTACACTAGTGTCATCCTCATCCAGATTCTTCTCTTCTTC AATCGGAAAAACTTCTTCGCTCTATAGATCCTCGATTCCTTCGTTTTCTCGCTTATCAGTGCGATCAATGGCGGATTCAG CTTTCAGGAAAATTCAGATCCAAAGAGATGACACTACATTTGATGCCTATGTGGTCGGTAAAGATGATGCGCCTGGGATT GTGGTGATTCAAGAATGGTGGGGTGTTGACTTTGAGATCAAGAACCATGCTATTAAAATCTCACAACTTGAGCCTGGATT CAAAGCCCTTATACCTGACTTGTATCGAGGAAAGGTTGGTTTGGATACTGCAGAGGCACAGCATCTAATGGATGGCCTTG ATTGGCCAGGTGCTATCAAAGATATCCGCGCTTCTGTTAATTGGCTAAAATCCAATGGCTCAAAAAAGGTTGGTGTCACT GGAATGTGCATGGGAGGTGCATTAGCTATAGCTAGCTCTGTATTGGTTCCTGAGGTGGATGCTGTTGTTGGATTCTATGG AACCCCTTCCTCAGAGCTCGCAGATCCAGCACAAGCTAAGGCACCTATTCAGGCTCATTTTGGAGAGCTCGACAATTTTG TCGGTTTTTCTGATGTCACGGCAGCAAAGAATCTCGAAGAGAAGCTGAAAGCGTCTGGAGTAGCACATGAAGTTCACATC TATCCAGGTAATGGACACGCGTTCTTGAACAGGAGTCCAGAAGGAGTGAGCAGAAGGAAAAGCATGGGACTTTCTGATGA AGATGAAGCCGCAGTGGAGCTTGCTTGGTCTCGCTTCACCTCCTGGATGAAACAGTACTTGGCTTAAGCTTTCTTCCATC AGGCAGCAATGGTTGATGTAAGTCCCAAAACGTTGTGTGATGCCCAATCATCTTTAAATAAGGACATTGCTCTTAGAACT TTTAAT ...............................................N................................ .......................................................N........................ .........................................................................i...... ................................................................................ .................................N.............................................. ...................................................................i............ ...........................N..............N.....N..............N..N............. ..............N...............................N................................. ...................................................................i...N........ ......................................................N......................... ...i.....i................................................N.................N... ................................................................................ ............N.....................................................N............. ..........N....................................................i............N... ..N...........................................N................................. ........N......N.......................N........................................ ...... Pos Score Pred ------------------------ 48 0.197 - 136 0.379 - 234 0.568 Yes 354 0.421 - 468 0.779 Yes 508 0.425 - 523 0.192 - 529 0.277 - 544 0.217 - 547 0.217 - 575 0.429 - 607 0.202 - 708 0.784 Yes 712 0.181 - 775 0.159 - 804 0.777 Yes 810 0.801 Yes 859 0.112 - 877 0.180 - 973 0.278 - 1027 0.300 - 1051 0.323 - 1104 0.518 Yes 1117 0.200 - 1123 0.340 - 1167 0.176 - 1209 0.301 - 1216 0.077 - 1240 0.070 -

Explain the output -- Processing locus 860: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACTAGTGGCTTCTGACGATTACTCCATCAATTTCATACTCACATGTATACATACACATACACACAACACTCACTCACAT ATACAATTTAAGTAGAGTTAGGGTTTTCTAATGTGTTTCATCACTCATCAAATGGTTATGAAACTATATGACTACGTTTC AGGCATGGGAACAAAGATCGTTGAGCGAATTACTACAAACCAAAGTGGCTTAAAGAGTGTTGATCATAGTGGGTTCATCA GATTTGTTGTTAATGGTCGACAAGAATCGTCAAAGGAGACGTTGACAGACGAGGAAGAAGAAGGAGTTTTTATTTTAAGG TCAATGGATAGGTTTCTTGAAAAGCAAAATGCTGAATCCATCAGACATACAATGCAAGCCCAAGAAGATATTTTTAAGCA ACAGGTACGAGAGCTACATAGAGTATATAATACACAGAAAATGATGATGACTCAACTCAAACATAGAAGCCAATATTGGA CTGTTAACAACAAAGACCAACCCGGTTCAAGAGAGAGAACCGGAAGTTGTTCAGGCATAGACCTCGAAAATGTGGTTAGA GCAACACAAACGACGACGGATCATATCGAAGAGAGCGAACTCGAATTGACATTGAGCATTGGATTGTCTTCTTCATCTAC TACTATGACCACGACAACGAACAAGGACATGGACTACTCGTCAACAACGTCAATGAGATCATCATCGGATAATTGTAATA ATCAGAGCAATAATAATCTATATAATAACAGCAATAATAACCAAGAAAGTAGCGGACCAAACACGCCTATGAGCAGCTCA AGTACGACGTCGTTGGATCGAGAGAAGAAGAGACCTCATTGGCTCTTTCAAGGACTTAGTATTAACCGAACCTCTTCTTG ATTTTTGTTAATTCACTGACTCTTTCTTTTTTCTTCTTCGTCTTCTTCTTGATTTTTTTATTTTCTTTTTGTATCTGATT TATCGTTATTATTATTTACG ...........................................i.................................... ..............................i....................i.....N.........N............ ....N........................................................................... ............N................................................................... ...N........................N......................i............................ ........................................i..N..N................................. .....................................................................N.......... ................................................................................ ....N.......................i.......................i........................... ....................................................................N........... ................................................................................ ................................................................................ .................... Pos Score Pred ------------------------ 44 0.502 Yes 111 0.547 Yes 132 0.582 Yes 138 0.327 - 148 0.169 - 165 0.355 - 253 0.103 - 324 0.250 - 349 0.401 - 372 0.594 Yes 441 0.615 Yes 444 0.357 - 447 0.456 - 550 0.245 - 645 0.195 - 669 0.580 Yes 693 0.582 Yes 789 0.166 -

Explain the output -- Processing locus 861: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGAATGATATACAAAGAAATCGGTTTATTTTCAGTTACGATTCTTTGGTTTATATGATCCGGTTTAATTGGTTACTAAG ATGATGTCGGAAAGAGAATAGGGAAGCGGCGTAACTAAAAAACACGACACGATGATGAATTCGGCGTTTCGAAACGTTCA CATCACTGCGTCTCGTTTGATAAACCCTAAGCCCTTTTCTTCTTCGATTCTCCATTTTCTTCCTCTCTGTTCCGGCGACG CTTCTCCTCGCCGTTCCAATCCTCTCCGGCATAGAAACTCGGTGTCTCCTTCATCTTCCTTTCCCTTCTGTTCGACTTCT TCCTTTTCTTCTTTCTACATTCCTATGGCGGGAGGAGATGGTCAGGTGTCTCATTCGCTTGAGAAGCAGTTCGAGAGTTT CCGTGTTCAATTGGAAGAATCTGCAGCTTTGAGAGAGCAGATTCGTGCTGTGGTTATGGAGATTGAGTCTGCTACTAGGC TTATTCAAGCTAATCTTCTTCTCGTTCATCAATCGCGTCCTATTCCTGAGGTTATCGAGAAAGCTAAGGAAAAAATCGTT GATTTGAAGCAGTATTATGGTCGGCTTGCTGAAATTCTTGAGGAGTGTCCTGGACAATACTATAGGTACCATGGAGACTG GAGAAGTGAAACACAGGCAGTGGTCTCCCAGCTTGCTTTTATGCACTGGCTAGAAACTGGAACTCTTCTCGTGCATACCG AAGCTGAAGAAAAACTTGGGTTGAACAGTTTGGAGTTTGGATTGGAGACAGAAGATTATCTCACTGGAATTTGTTTCATG TCAAACGATTTGCCTAGGTACGTGGTTAATCGAGTGACAGCAGGAGACTATGACTGCCCAAGAAAGGTGATGAATTTCCT GACAGATCTTCATGCAGCCTTTCGCATGCTCAATCTCAGGAACGATTTCCTGCGCAAGAAATTCGACAGTATGAAGTACG ACCTAAGAAGAGTGGAAGAAGTTTACTATGATGTCAAGATCCGAGGTTTGATTTCCGGCGGAGATCCTCCTGGCGTCCAA GCAGTCCAAGGCCAATCCTAAATGTCCTCGGCTCTTTTTAAGTTTTTATGTATAACTTTGTTTAGCTAGCATGAAAACGT CAAGGAGTGTTCTTCTCTAAACCAAAAACGGCAAGGAAAA ....N.................................................N......................... N..N...............................................N..N......................... ................................................................................ ................................................................................ ........................i............N.......................................... .......................................................i........................ ................................................................................ ................N.....................................................N......... ........................................N....................................... .............................................................................N.. .................................................N...................i.......... ...........N.............N............................................N......... ...........................N..N................................................. .....................i.........................N......................N......... ........................................ Pos Score Pred ------------------------ 5 0.412 - 55 0.275 - 81 0.279 - 84 0.320 - 132 0.161 - 135 0.354 - 345 0.817 Yes 358 0.329 - 456 0.802 Yes 577 0.481 - 631 0.385 - 681 0.305 - 798 0.380 - 850 0.254 - 870 0.505 Yes 892 0.173 - 906 0.335 - 951 0.488 - 988 0.311 - 991 0.337 - 1062 0.590 Yes 1088 0.063 - 1111 0.227 -

Explain the output -- Processing locus 862: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACGAAATGCCTGAAAGAGCTGTTTTGTTGTATGATTCTGTATGTTTGTGTTGAGCATAGTACAATCTAGTTTTATGGTC ACCTTTAGCTTTGAATGATGAGTTCTAAATAGTGATTTGATGATGAGTGTAGAGAGACCCATCTCCATGGCACCAAGGTG GAAATGGAAAGGTGCTGAGGCAAAGGCACTTGCAGAACCTGTTTCGAAAACAGTCTCGGAGCTGAGATCTTCATTGACCC AAACCGAGGCTTTAGGTTTCCTCTCAAGTTGTAACGTACTTTTATCGGTTGAGTCAGAGGAAGCTGAGCTTTTGGATCGT TGTTGTTTTGGTAGACTAGTTGTTGGTGCTGAGAAAGATAAGAGATGGATTCAGTTATCCTTTGAAGAAGCTTTCTTTTT GTTTTACAAACTGAAATGCATCAAGATTTGCCTTCACGGTCGTTCTCTGGAAAACGAGGTAGATTTATGGCGATCCATGA GTTCCTTTAAGCAAGATTTTGCGATCTTGTACAAGGCTTATTCGCATCTACGATCGAAGAACTGGATTGTGAGATCAGGG TTACAATATGGAGTTGATTTCGTGGTGTATAGACATCATCCATCTCTTGTCCACTCTGAATACGCCGTTCTTGTTCAGTC CATTGGCGGGAATGATCGGTTAAAGGTGTGGTCCGATATCCATTGCAGTGTTCGACTAACCGGAAGCGTCGCGAAATCAT TGTTGGTTCTTTACGTCAACAGAAAAGTCAACACAGAGAAGATGAATCTGCCTTTGTGTTTAGAGGACTACACAGTTGAA GAGCAAACAATACGTAGATGGAGTCCAGAACTTAGCCGTGAAGATGAAACCAGAACATGATTAAGCTTACATGTTAACAA GTCTTGTTGTAATGCTGATTTCTAGAAGAATATAAAATGAACTAGGTTCCGATTCCAACATAAATAATATACACACAAA ......i........................N.........N................................N..... ..............N..i.....................i..i.......................N............. ...i............................................................................ ................................................................................ ............................................N................................... ...............N..................................................i.........N... ................................................................................ .......i........................................................................ ...........N.................................................................... .........................................i...................................... .................N.........................N............N.............N......... ...........N........................N.......................................... Pos Score Pred ------------------------ 7 0.508 Yes 32 0.247 - 42 0.331 - 75 0.120 - 95 0.403 - 98 0.728 Yes 120 0.739 Yes 123 0.810 Yes 147 0.473 - 164 0.689 Yes 365 0.303 - 416 0.372 - 467 0.685 Yes 477 0.360 - 568 0.651 Yes 652 0.462 - 762 0.739 Yes 818 0.234 - 844 0.497 - 857 0.390 - 871 0.105 - 892 0.119 - 917 0.085 -

Explain the output -- Processing locus 863: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 864: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATCCAAAAACCAGAAAAAAAACGTGTTATGTTTTTATACTAAAAGAAAGTTTATTAAAAAAAAAAACCTCAGAATCAAA GCTCTCTCATTAGCACTTTAGCAGTCAGTCTATAAATACATGTCTCACATCCACACAAAAAGAAGAAGTAAGTATCAAAC GAGAGTACAAAAGATGACGTCACTCAACAGCTCTGCATCACCAACATCATCGTCATCAGACCAATCTGATGCAACTACTA CAACAAGCACCCACTTGTCTGAAGAAGAAGCTCCACCCAGAAACAACAACACAAGAAAGAGAAGGAGAGATTCTTCTTCT GCTTCTTCATCTTCTTCAATGCAACATCCTGTTTACAGAGGTGTGCGGATGAGAAGTTGGGGCAAATGGGTCTCCGAGAT CCGACAACCTCGTAAGAAAACTCGTATTTGGCTCGGCACTTTTGTCACCGCTGATATGGCTGCTCGTGCTCACGACGTCG CTGCTCTCACCATCAAAGGCTCCTCCGCCGTCTTAAATTTCCCTGAGCTTGCTTCTCTCTTCCCTCGTCCGGCGTCATCA TCGCCGCATGATATCCAGACAGCCGCCGCAGAAGCCGCCGCCATGGTGGTCGAAGAAAAACTGTTAGAGAAGGATGAGGC TCCGGAGGCCCCACCTTCGTCGGAATCTTCTTACGTGGCGGCGGAGTCAGAGGATGAGGAGAGGTTGGAGAAAATTGTGG AGCTGCCTAACATTGAAGAAGGAAGTTATGACGAGAGTGTGACATCACGTGCTGATCTGGCTTATTCTGAGCCGTTCGAT TGTTGGGTGTATCCTCCGGTTATGGATTTTTATGAAGAAATATCGGAGTTTAATTTCGTGGAATTGTGGAGCTTTAATCA CTAATTAAGTTAGGAAAGTGCATTATATTGCAATATTGCATCATAGATAACATTTGTATTTCTTTTCTTTTTGTACGGAT ACGTAGCATATGCTACTATACTA ............................N................................................... .......................................N........................................ .............i......................................................N........... ................................................................................ ..................i.............................N................N.............. .......................................................i........................ ................................................................................ .......N..................................i..............................N...... .....................................................N.......................... ...........................N.................................................... .....................N.........N................................................ ................................................................................ .........N............. Pos Score Pred ------------------------ 29 0.383 - 120 0.219 - 174 0.864 Yes 229 0.238 - 339 0.598 Yes 369 0.447 - 386 0.334 - 456 0.726 Yes 568 0.149 - 603 0.870 Yes 634 0.304 - 694 0.182 - 748 0.328 - 822 0.411 - 832 0.148 - 970 0.066 -

Explain the output -- Processing locus 865: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCGATTATCCGGTTTAGTAGCTTCAAGTGTTTCCCCTGTGTCCATGTATCGTCTTGGTCCTGATGATTTTGAAATGTTC TGTTTTGTTTGTTTATGTTAAGAACAGAGTTTGAAGCTTTTTTTAGAGACTTCAATGGTGAGCCAAGGAATGGTATCTCT TACAAAGTCTCTATGCACTATGACTCCAAGAGTCCGTCTCAAGAATCCGAATATGTTGCAGAAACTAAAGACTGGATCTT GTAATTTCCGTTTCAGAAATCTCCGTGTCTTATGCACACCAAAGCTGTCTCAGTGGGAACCGTCACCTTTCATTCATGCT TCTGCAGAAGAAGCTGCTGATATAGTATTGGACAAAACCGCGAATGTGTTCGAATCTATTGTCTCCGAGAGTGCAGAGGA AGAGAAAGTAGATATGTCAGCGCAACAACGAACTAATTCTCAAGTTCAGGTACTTAAATGGCCAATATGGCTTTTGGGTC CATCTGTTCTCCTCACAAGCGGTATGGCGCCTACTTTATGGCTTCCGTTGTCATCGGTTTTTCTCGGTTCCAACGTGGTT AGTCTACTTTCTTTGATCGGTCTAGATTGCATTTTCAATCTTGGAGCAACCCTTTTCCTCTTAATGGCTGATTCTTGCGC ACGTCCTAAAGACCCATCACAGTCCTGCAACAGCAAACCACCATTTAGCTATAAGTTCTGGAACATGTTTTCACTCATAA TCGGGTTTCTAGTCCCAATGTTACTTCTCTTCGGATCCCAATCTGGCCTTCTCGCTTCTCTCCAGCCCCAGATTCCTTTC CTCTCGTCCGCTGTTATCCTCTTCCCGTACTTCATTCTCCTTGCTGTTCAGACATTAACAGAGATCCTCACATGGCACTG GCAATCACCGGTCTGGCTAGTCACACCAGTTGTCTACGAGGCTTACCGAATCTTGCAACTGATGAGAGGGTTGACACTTA GTGCAGAGGTCAACGCACCGGTTTGGGTGGTTCATATGCTTCGTGGGCTTGTCTCTTGGTGGGTATTGATCTTGGGAATG CAACTCATGAGAGTTGCTTGGTTTGCTGGTTTTGCATCTAGAACAACAACAGGTCAGCAACCACAATCTGTTGCTTCTAA GTAAAGACAGATAAATGTTTCTTCGTTTAGTTTTTAAAGTCATGTCAAAGATTTGTAACTCTTTCTTCACTGTATAGCTT TTATTATTCGTGGGTTGGTGATA ............................................N..................N..........N..... ..............N.......................................N..............i.......... ............N......N................................N........................... ...............................N...........................................N.... ...........................................N.................................... .............i...........................................i........i............. .......................N.............N.......................................... ...............................................................N................ ................................................................i............... .................N.............................................................. .......................................................................i........ .............................................................N.................. ...................................N.........................................N.. ......N......................................................................... ..............N..........................N...................................... ....................... Pos Score Pred ------------------------ 45 0.206 - 64 0.348 - 75 0.243 - 95 0.414 - 135 0.257 - 150 0.500 - 173 0.259 - 180 0.310 - 213 0.365 - 272 0.167 - 316 0.153 - 364 0.316 - 414 0.783 Yes 458 0.705 Yes 467 0.709 Yes 504 0.293 - 518 0.320 - 624 0.316 - 705 0.519 Yes 738 0.183 - 872 0.891 Yes 942 0.383 - 996 0.403 - 1038 0.145 - 1047 0.172 - 1135 0.089 - 1162 0.053 -

Explain the output -- Processing locus 866: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATGGGCTGCACTACTTGATATTATATCCAAAACCGATGAAAGATTTACAAATGGACCAAATAGTAATCAGGCCCAATGG GCTAAATCGTATATAGTCTGATATTATGATCTGTCATGCCACGTCATAGGCATGATCCATAACGAATTGATAAAACTCTA TGGTTGTCCAAGTTCGAACAAAAATCTGAAACACACTTCCATGGCTTCGCTTCCTGTTCAATTCACGAGGAATCAAATCT CTTCACCGTTTTTCTCTGTAAACCTCCGCCGTGAGCCTAGATCTTTAGTAACAGTACACTGCTCCGCAGGAGAAAACAGA GAAAATGGTGAAGGTGTAAAGAAGAGTCTCTTTCCTCTTAAGGAGCTTGGATCTATCGCTTGCGCAGCTCTCTGTGCTTG CACTCTTACAATAGCTTCTCCTGTTATTGCTGCTAACCAGAGACTTCCTCCGTTATCAACAGAACCAGACCGGTGTGAAA AAGCATTTGTTGGTAACACGATAGGTCAAGCAAATGGTGTCTATGACAAACCACTGGATCTTAGGTTCTGTGATTACACA AATGATCAAACTAATCTCAAAGGCAAGACTCTCTCTGCAGCCTTGATGGTTGGGGCTAAGTTTGATGGTGCAGATATGAC TGAAGTTGTTATGTCAAAAGCTTATGCTGTAGAAGCAAGCTTTAAAGGGGTGAATTTCACTAATGCTGTTATCGATCGGG TGAATTTCGGGAAATCGAATCTGAAAGGTGCGGTGTTTAGGAACACGGTATTATCCGGTTCGACATTTGAGGAGGCAAAT TTGGAGGATGTGGTCTTTGAGGACACTATCATTGGTTACATAGACCTTCAGAAAATATGTAGGAATGAGTCCATAAACGA AGAAGGAAGACTTGTGTTGGGATGCCGATAGAGAAGAGAGAAGGTTTTGATTTGAATGTATTATATATGTAAGATTTAAG ATTTGTTGACACAAAGATGTATGAAACTAAGTAAAGGTTGAAGGATAGGA .........................N.........N...............N...........................N ........................................i....................................... ................................................................................ ....i........................................................................... ................................................................................ .................................N........N..................................... ..........i............N......................................N................. ................................................................................ .......N................................................N.......N............... .....................i.................................N..........N............. ................N...N............................. Pos Score Pred ------------------------ 2 0.208 - 37 0.334 - 52 0.626 Yes 77 0.503 Yes 106 0.193 - 116 0.236 - 132 0.328 - 160 0.270 - 201 0.610 Yes 325 0.591 Yes 514 0.285 - 523 0.265 - 562 0.118 - 606 0.674 Yes 625 0.118 - 636 0.845 Yes 651 0.836 Yes 664 0.086 - 703 0.107 - 808 0.373 - 857 0.306 - 865 0.391 - 902 0.515 Yes 936 0.052 - 947 0.084 - 977 0.093 - 981 0.031 -

Explain the output -- Processing locus 867: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCCTTGTTGGGAACGAGTTTGTAACAGTTGCTTTGAAGGAGACTCTTTTGGCACTGATGTTAATACCGTCCCTTCGACTA TCGAGAAAGGTTAGGTCTTGAAGCAATGTGTTTGTGTGTGTGTGTTTTTTTCTGATGCATCTATGTTTTATTGATATGTC CTTGTTTTATGATTTCTTAGCAGCTTGTCCTCCCATTTTATCACCTTGTAAGTACTCTAACAGGAATCTTAAGGATAATC CAGCTGAGAGCAGAGAGAAAAGCAATGTGACTGAGACTGATATTGCAGAGATTAATGATAAAGGTGGTTCTGGAGCAAGA GATATAAAAACTGCAAGGAGAAGGTCTCTTCATCTGCAAATAAAAAGGATACTTGAATCGAGTAAAGTCCGGAAGACTGC TAATGATGGAGATCATGGTAGTGAATTTTTGAATACGGCTAAGCGCGGTGATGTAGAAAGAGATGGATGTGAGGTTATCA ATAATGAAGACAGTGAATGGAAACTCGATGAAAGTGAAGTCCAGAATCTTTGTAATGATGGAGATAATGGTAGTGAAGGT TTCATTAAGGCTAAGAGCAGTGATGTAGAAAAAGATAAGAGCGAAGCTATCGATAATGATGTGATATCTCCAGCGGTTGG AAGTGGTATTAAGCATACCGGTGCAGATAATGTTGATAAAGTGACAAGTGCAAGTGCTACTGGAGAATCACTTACTTCGG AACAGCAAAATGGTTTACTTGTAACAACAGCATCTCCACATTCCCTGCTTAAAGACTTAGCCAAGAGTAGCAAACCTGAA AAGAAAGGAATATCCAAGAAAAGTGGCAAGATCCTCAGAAGTGACGACAATGTAGTAGATCCCATGAATTACTCTGGGAC GAAAGTCAAAAGTGCGGAAAACAAAAGGAAAATCGATGCGAGTAAACTCCAGAGTCCGACTAGTAATGTTGCAGAACATA GTAAGGAAGGTTTAAATAATGCTAAGAGCAATGACGTAGAAAAAGATGTATGTGTGGCTATCAATAATGAAGTGATATCA CCAGTGAAGGGATTTGGTAAAAGGCTTTCTGGTACAGATGTTGAAAGATTAACAAGTAAGAATGCTACTAAAGAATCACT GACGTCAGTACAGCGAAAAGGTAGAGTGAAGGTATCGAAGGCATTTCAAGATCCCCTGTCGAAAGGAAAATCAAAGAAAA GTGAGAAGACCCTTCAAAGTAACAGCAATGTTGTAGAGCCTATGAATCATTTCAGGTCTGAAGCTGAAGAAGCTGAAGAA AACTTGTCATGGGAAAAAATAAAGAGGAAAATCGACTTTGATGTGGAGGTAACACCGGAAAAAAAAGTGAAGCAGCAGAA GACCAATGCGGCGTCTACTGATTCATTGGGACAGAAACGGTCAAGATCAGGAAGGGTGCTTGTGTCATCACTAGAGTTTT GGCGTAACCAAATTCCTGTTTATGATATGGATCGGAACCTTATCCAAGTAAAGGATGGTAGTGAGACTAACTCCGCTCCA TCTAAAGGAAAAGGATCGGATTCTCGAAAGCGAAGAAACTTGAAAATCAAATAAACTATTTCAAACTTTCGTAATTAATA AGTAGGTTTATTATGGTCTCTGACCCTGTAATCATAATTAATAAGTAGGTTTGTTATGGTCTCTGACCCTGTC ........................................................N....................... .........................N............................N.......N............i.... ........N....................................................................... ........................N.............................N......................... ................................................................................ ..N..N........N...................................N...........N...N............. ...N............N..........N..........................N..N........N............. ......................N................................N..N..................... .............................N.................................................. .........i...................................................................... .................................................N.............i................ ...................................N.............................N.............. ..................N...........N..............N...N................N............. .....................................N.......................N.................. ................................................................................ ...........................N.............i...................................... ........N...............................N....................................... .....N.......................................................................... .....................N....N...........................i......................... ................................................................................ ............N..........................................N................. Pos Score Pred ------------------------ 57 0.111 - 106 0.145 - 135 0.154 - 143 0.172 - 156 0.585 Yes 169 0.452 - 265 0.310 - 295 0.196 - 403 0.452 - 406 0.465 - 415 0.438 - 451 0.309 - 463 0.305 - 467 0.140 - 484 0.164 - 497 0.202 - 508 0.153 - 535 0.239 - 538 0.132 - 547 0.200 - 583 0.234 - 616 0.084 - 619 0.136 - 670 0.142 - 730 0.594 Yes 850 0.204 - 864 0.648 Yes 916 0.158 - 946 0.111 - 979 0.192 - 991 0.340 - 1006 0.315 - 1010 0.447 - 1027 0.224 - 1078 0.186 - 1102 0.430 - 1228 0.324 - 1242 0.550 Yes 1289 0.396 - 1321 0.239 - 1366 0.180 - 1462 0.376 - 1467 0.319 - 1495 0.558 Yes 1613 0.061 - 1656 0.058 -

Explain the output -- Processing locus 868: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAGAAATCTAACAGTCAATGGAACTCAGAGAACGACCAAAAACTAACAGTCAATATGCAAAGATTATCTGTGGGAAACT TACCATATATATTAGTGTTGAACCAAGATGCTGAGAGTAAGAAAGGCAATGGAGAATAAGAACCAGAAGCGGTTTGTGCT CATCCATGGGGTGTGCCACGGGGCATGGACTTGGGACAAGGTGAAAACGCAGCTGGAGGTTGCAGGTCACTGTGTGACGG CAGTGGATCTTGCTGCATCAGATGACGGTAAGGTGATTGTTGTTGCTCATAGCATGGGAGGAATATCCGCTGCACTTGCT GCTGACAGCTTCGCTTGTAAGATTGCCGCTATTGTCTTTTTGACAGCTTTCATGCCCGACACAATAAACCCACCTGCTTA TGTTTACGAAAAGCTGCTCAGAAGCATTCCACAAGAGGAATGGTTGGACACCACGTGTGTGAACTACGGGAAACCTGATT TTCCTCTACAGTATACTCTTTTGGGACCAAAGTTTATGGCCAAGAAAATGTATCAAAACTCTCCAGTTCAAGATCTTGAA GTGGTGAAGACATTAGTGAGGGAAAACCCGTTAGTTACAAACAATCTGGCAGGGACAAGAAGCTTTAGTGAGGAAGGGTA CGGATCCGTTACACGTATATATATTGTATGCAGAGAGGATCTTGTGGAAGTCGAAGATTACCAGCGTTGGATGATTAGCA ACTTTCCACCAAAAGAAGTAATGGAGATCAAATGTGCAGATCATATGCCAATGTTCTCCAAGCCTCAAGAAGTTTGTGCT CTTCTCCTGGAGATTGCAAATAAATATTGCAAAAATTAACCAATTTCAGCTTCCACCAATAAATAATACGCCATAAGAGA ATACATCTATATGTATGAGAACTAAGCAAATGATAATGCTTATATAATAAAGTTTCGT ..................i....................................i........................ ...........................N....................i............................... .....N..................N....................................................... .....................N...............................i.......................... ...................................................i...........................N .......................................i........................................ ...................................i...........N................................ ................................................................................ ...........................N..........................................N......... ....................i..........N............N.....N............................. ................................................................................ ..........N...N..............N.....N...................... Pos Score Pred ------------------------ 19 0.663 Yes 56 0.605 Yes 108 0.423 - 129 0.565 Yes 166 0.323 - 185 0.269 - 262 0.109 - 294 0.638 Yes 372 0.631 Yes 400 0.189 - 440 0.736 Yes 516 0.692 Yes 528 0.455 - 668 0.391 - 711 0.166 - 741 0.593 Yes 752 0.221 - 765 0.340 - 771 0.479 - 891 0.148 - 895 0.050 - 910 0.047 - 916 0.055 -

Explain the output -- Processing locus 869: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATGGGCTGCACTACTTGATATTATATCCAAAACCGATGAAAGATTTACAAATGGACCAAATAGTAATCAGGCCCAATGG GCTAAATCGTATATAGTCTGATATTATGATCTGTCATGCCACGTCATAGGCATGATCCATAACGAATTGATAAAACTCTA TGGTTGTCCAAGTTCGAACAAAAATCTGAAACACACTTCCATGGCTTCGCTTCCTGTTCAATTCACGAGGAATCAAATCT CTTCACCGTTTTTCTCTGTAAACCTCCGCCGTGAGCCTAGATCTTTAGTAACAGTACACTGCTCCGGAGAAAACAGAGAA AATGGTGAAGGTGTAAAGAAGAGTCTCTTTCCTCTTAAGGAGCTTGGATCTATCGCTTGCGCAGCTCTCTGTGCTTGCAC TCTTACAATAGCTTCTCCTGTTATTGCTGCTAACCAGAGACTTCCTCCGTTATCAACAGAACCAGACCGGTGTGAAAAAG CATTTGTTGGTAACACGATAGGTCAAGCAAATGGTGTCTATGACAAACCACTGGATCTTAGGTTCTGTGATTACACAAAT GATCAAACTAATCTCAAAGGCAAGACTCTCTCTGCAGCCTTGATGGTTGGGGCTAAGTTTGATGGTGCAGATATGACTGA AGTTGTTATGTCAAAAGCTTATGCTGTAGAAGCAAGCTTTAAAGGGGTGAATTTCACTAATGCTGTTATCGATCGGGTGA ATTTCGGGAAATCGAATCTGAAAGGTGCGGTGTTTAGGAACACGGTATTATCCGGTTCGACATTTGAGGAGGCAAATTTG GAGGATGTGGTCTTTGAGGACACTATCATTGGTTACATAGACCTTCAGAAAATATGTAGGAATGAGTCCATAAACGAAGA AGGAAGACTTGTGTTGGGATGCCGATAGAGAAGAGAGAAGGTTTTGATTTGAATGTATTATATATGTAAGATTTAAGATT TGTTGACACAAAGATGTATGAAACTAAGTAAAGGTTGAAGGATAGGA .........................N.........N...............N...........................N ........................................i....................................... ................................................................................ ................................................................................ ..............................N........N......................................N. ..........................................i..................N..........i....... .......i............N......................................N.................... ................................................................................ ....N................................................N.......N.................. ..................i.................................N..........N................ .............N...N............................. Pos Score Pred ------------------------ 2 0.208 - 37 0.334 - 52 0.626 Yes 77 0.503 Yes 106 0.193 - 116 0.236 - 132 0.328 - 160 0.270 - 201 0.610 Yes 322 0.672 Yes 511 0.285 - 520 0.265 - 559 0.118 - 603 0.674 Yes 622 0.118 - 633 0.845 Yes 648 0.836 Yes 661 0.086 - 700 0.107 - 805 0.373 - 854 0.306 - 862 0.391 - 899 0.515 Yes 933 0.052 - 944 0.084 - 974 0.093 - 978 0.031 -

Explain the output -- Processing locus 870: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTATTATAGAAGATTTTAGTTTAGCAAAACTCTATTTTAGCAGAACACATAAAAGGAACAAGGATCAAATCCTTTATG TTATGGATATAACTAATATAAATTTGTGACATAAAAATTAGGATAACAAGAATGAAATCCTTTAAACTCAATAAGGAACA CAGATATCACACCAATCAAACAACTCCACGTGTCGATTCCTCACTCCTTCTCAAACCACAGCCTAATCCTTCTGAAAACT CCACACAAGATAAACCTTCTAATCTCCACCGTCCATCTAACCACAATCCAAAATCTCCACCGTCCATTCTCATCTCCCCC AAAAAACCTTACCTTCTTCTTCTTCTTCCTCTCCTCTATCGTCTTCGAGAAACCACAAATCTCTCTTTCTCTCAAACTCT CTCAACAACAACAATGGCGTCTCTTCAACAAACTCTATTCTCTCTTCAATCCAAACTCCCACCATCCTCCTTCCAAATCG CCAGATCTCTCCCACTCCGAAAAACCTTCCCAATCCGAATCAACAACGGTGGAAACGCCGCCGGAGCAAGAATGTCAGCC ACCGCAGCATCAAGCTACGCGATGGCATTAGCAGACGTCGCGAAAAGAAACGACACAATGGAATTAACAGTCACAGACAT CGAGAAGCTCGAACAAGTCTTCTCAGATCCACAAGTACTAAACTTCTTCGCGAATCCAACAATCACCGTCGAGAAGAAAC GTCAAGTCATCGACGACATAGTGAAATCGTCGTCTCTTCAATCTCACACATCTAACTTCCTCAACGTCCTCGTCGACGCG AATCGGATCAATATCGTGACGGAGATCGTTAAGGAGTTTGAGTTGGTTTACAATAAGCTAACGGATACACAATTGGCGGA GGTTAGGTCGGTGGTGAAATTGGAAGCGCCGCAATTAGCTCAGATTGCGAAACAGGTTCAGAAGTTAACCGGAGCTAAGA ATGTTCGGGTTAAGACGGTTATTGATGCGAGTCTTGTGGCTGGTTTTACGATTCGGTATGGTGAATCCGGTTCGAAGCTT ATTGATATGAGTGTGAAGAAACAGCTTGAAGATATTGCTTCTCAGCTTGAACTTGGTGAGATTCAATTAGCTACTTGAGA TTTGGGAAAAATTGTATAAGAGAAAAATTTGAGAATCTTTTTTTTTTGTGCAAGTTTAATTTTTTTTCTCCTCATCTTCT TTCTCTATTAATCAATC .............................................................................N.. ..N................................................N............................ ................................................................................ ................................................................................ ................................................................................ .............i.................................................................. .......................................................................i........ .....................i...................................i...................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ N.......................N................................N...................... ......i......................................................................... ................................................................................ ................. Pos Score Pred ------------------------ 78 0.126 - 83 0.270 - 132 0.479 - 414 0.805 Yes 552 0.866 Yes 582 0.616 Yes 618 0.717 Yes 961 0.370 - 985 0.096 - 1018 0.093 - 1047 0.753 Yes

Explain the output -- Processing locus 871: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCAAATTTCTGCACTTGATCTTTATACATCTTAGATTCATACCGAGATTTCATCCGTTTTCCATTTTAGAATCTTCATT TCAATTCAAGTTCGTGTTTAAATGGAATTCTCATTGATCTCCGATGAAAATTTCATCTTTAACAACGCTTATCTATGTTC TGTTAATGATGATACATCTCATATACCAAAAAATAATCTTACCTTTTTCAATGACAATCTAGGTCAATCTTCTTGTACTA GATGGTCCTTTTCACCAGATCTGACTGATATCTCTAATCAGCACTACCACCAAAATCTTATACCTTTGATCCCAAATTAC GATTCTCAAAATCAGAATTTGGATACTAATCAAAACCATTTGGTTTACAACTCTTCCTCATATAAAATTCCATCAAACTA CCCATTAATGAGTATCAAATCCTACTCAAATGTTGATATCTCTGAGCAATCTATGAATAGCATAGCCAACAACGGAAAAA TCCATATGATTGATAACCCCCCAACTTTTGCATACCCTAAAGGTATATTCAAAAAAATTTATGATTTGCGGGGAAATACT TTAAGAGATGAGATTGTATACAATGAAGAGCTGTCTAATAAGGGATATGAGCCAACTCTTGATCAGGTAATGGGTGAACC TCAATCATTTAATGTGCCAGTGCCAGAATATATCAAAAACACAACCAACGAAATCATGAATCAAAGTGAAGATGATAAGA TGAAAAAAAATTATCAAAAGAAGAATATAAAGGTGGTGAGCACAAGCAAATATTTGAAGAAAAGTGACATCGACAAAGTA AACTGGACAGAATCTGAGGACATAAAATTGAAAGAGATAATGGCGCTTGGACCCAAAAACAAATGGACAAAGGTCGCAAA AAAATTCGAAGGAAGAACAGGAAAACAATGCAGAGAGAGGTGGTATAACCATGCTCGTCCTAACATTAAGAAAACTGCTT GGAGTGAAGAAGAAGATCAAATTTTGATTGAAGCACACAAAGTACTTGGTACCAAATGGGTCGAGATCGCACAGCAACTT CCGGGGAGGAGCGATAACAATATTAAGAACCATTGGAACACCACAAAGCGTCGGGTGCAAAATAAAAGAGGTGGAACTGT CAACCCTGTCGGCAATAACATCCTTGAAAACTACATAAGGTGTATCACAATCAACAACGAAGATTTTCTTAAGACCGATG GATCTTATGGTGAACCGACAAACATTGAGAGTGATGATGACTCTAAAGATATGTTATACGGGGAGATGAATTTGAGTCTA GAGACTATAACGACGCAGACCACCAAACCATTGACCAATGCTTCTACAACATCACCTTATGTTCAGATGCCAGAAGATAA TTATACTATGGAGGATTGTGAATCACTGGAGGACATTCTAGAGCTGCTGCGTTGGTGGGAATAATTTCCAGTGAAGGAAA TTTCTTTGATATATGATATTTTCTTACTATCGTGTAAGATAATATATCTATCTATCTATCTATTTTGTTAAAAGCGATTT ATA ................................................................................ .....................i.....................N..............................N..... .....N..N.........................................N............................. ................................................................................ .......N.....................N......................i........................... .....i......................................................N................... .......N..............N.......................N......................N.......... ...........N...........................................i...............N.......i ................................................................................ .......................................i......................N................. ...........................N......................N............................. .......................................................i........................ ................................................................................ .............................................................................N.. ......N..........................N..N.............i..............i.............. .....................................N....................N.......N............. .......N........................................................................ ............N................................................................... ... Pos Score Pred ------------------------ 102 0.550 Yes 124 0.160 - 155 0.150 - 166 0.168 - 169 0.116 - 211 0.218 - 242 0.141 - 408 0.428 - 430 0.330 - 453 0.694 Yes 486 0.734 Yes 541 0.183 - 568 0.274 - 583 0.475 - 607 0.202 - 630 0.462 - 652 0.180 - 696 0.575 Yes 712 0.198 - 720 0.612 Yes 840 0.578 Yes 863 0.419 - 908 0.478 - 931 0.433 - 1016 0.820 Yes 1198 0.353 - 1207 0.206 - 1234 0.104 - 1237 0.144 - 1251 0.563 Yes 1266 0.619 Yes 1318 0.227 - 1339 0.266 - 1347 0.450 - 1368 0.272 - 1453 0.052 -

Explain the output -- Processing locus 872: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAACATTTGGAAGAAAAAAAAACATGAGTTGTAAAATATATTAATCCCAAGAGCAATGACAAAGTTTTGTACTTTTCAT GTCTACTTCTTATCTTTGTAGATAGAAAATTACGAATATCGAAATAACCATCTTGAATATACACGTATGAGTAACGAGCC CATATATTTATGTTCGTATGTTTATGGGCCTAATGAGATTAAGAGAATGGATTGGCCTACGACGACTCTCATCTCCCGAA GAATCTGTAATCTTTTGATTTTCTTCGTCAAATTAAACCACAAAAACCTCTCCATCGATTCGGATAATCCACAACACTCT GCGATGAAACCCGACCCGACCAGACGCAGAAACAGAGCCTTATCTTCTTCTTCTCCATCAAAGACCCGACCCGAACTATC TTCCGGGTCTCCCAACCGGAATACCGGTGAAGCCCGTGGTGGAAAGGTGAAAACTTCGGCCACTTTACTCGACCGGGAGG AGATGGGTCTTTTTCCCGGGTCGGGTTACGGTGACCCGAATCCAGAACCTAGGAGCTTTCCTTATAGTGTGAAGCAGCAA TGTTGGGAAAAAGCAGAGAAGATTAAAGGAAGAGATCCTGAGCGTTGGAGGAGAGATCATTTAGGGAACATTGTGTTTAG GAAGCTTGTTGGTTGTCCTGGATGCTTGTGTCATGATTATGATCACATTGTTCCTTACTCCAAGGGCGGCAAGAGCACTC TGGAAAACTGTCAGGTTTTGCAGGCAAAGGTAAACCGATCAAAGGGTAATAAAACCGATATTTCTCGATCCGAGCTTATC CAAAGGAGTTCTTACTGTCGAGTCGCTGGCCGAGATATGGATCTGATTGAGCTAACAGCTTACGGGAATGTACAGCGTGC ACCTACGTCTAGCGGATGCAGAATTCAGTGATCTGTTGAAGATCTATTGATGGAATTGAAAATGATGAGTTTCTCAATAT TTACTTGGTCAAGAATCTGTAAACTTGTAGTTCTTCTCCGAGTTACTTGT ........................i...............................i.....................N. ..................................................................N............. .........N.......N.....N........N.............i................................. ................................................................................ ...i............................................................................ ................................................................................ ..i............................................................................N ................................................................................ .....................N..........N.....N......................................... ................................................................................ ....................................i..............................N............ ...............N.................................N...........N..N............... .................................................. Pos Score Pred ------------------------ 25 0.507 Yes 57 0.577 Yes 79 0.172 - 147 0.485 - 170 0.193 - 178 0.180 - 184 0.172 - 193 0.310 - 207 0.670 Yes 324 0.535 Yes 483 0.566 Yes 560 0.439 - 662 0.179 - 673 0.279 - 679 0.301 - 837 0.558 Yes 868 0.090 - 896 0.244 - 930 0.157 - 942 0.114 - 945 0.101 -

Explain the output -- Processing locus 873: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAAAAGATTACTTACGATAACATAGCCATCAGAGAATCCAAACATCACATGCCTCCCTATATGTATGTGTCTCTCTTGT CTTGTGTGTGTGTATATATGAGACATTAGTTATAGAAGAGAGACTAACATGAAGATGGATTTTTCATGTTTCCAAGAATA CCCTTTTGAGTTTCATTGCAGAGGAACAACATTTAATGGGTTTAGAGAAAACAATGCAGTGTCTGAAACAGTAGAAGAGT TCTGTAATAAAAGAAGGATGCAGAAGAAGAGTGATGATTTGAAGACTAAGAAGAAGAAGAAACAGAGTGTTTCTAGGGTT TGTAGTAGAGGACATTGGAGGATCTCTGAAGATACTCAGCTTATGGAGCTTGTTTCGGTTTACGGTCCTCAAAACTGGAA CCACATTGCAGAGAGTATGCAAGGAAGAACAGGAAAGAGCTGCAGATTGAGGTGGTTTAACCAGTTAGATCCGAGGATTA ACAAGAGAGCTTTCAGTGATGAAGAAGAAGAGAGACTACTTGCTGCTCATAGAGCTTTTGGTAACAAATGGGCTATGATT GCTAAGCTTTTCAATGGAAGAACAGATAATGCCTTGAAGAATCATTGGCATGTTCTCATGGCAAGGAAGATGAGACAGCA ATCAAGTTCTTACGTCCAAAGATTCAATGGTTCTGCTCATGAATCTAACACAGATCACAAAATCTTCAATCTTTCTCCTG GTTTGTCTCTTCTTACCTTACACATATGCATTGAGTTTAACTCTGTTATTGTAATGAGATACTTTCGATATTTATCACTC AGGAACAATGAACTTATGGTTTGGTCTCAAAAGTAGTCAGATTGCAAGTTTGGTGAGTCTTTAAGTTTCATGGTTCTGTG TGTTCTTGCAGGTAATGTAGATGATGATGAAGATGTGAATCTGAAAAAGTGCAGC .................................................N...........N...N.............. .................N..............................N.....i..........N.............. ...................................N.................N.......................... .................i...............N.............................................. ..........................................N..................................... ................N............................................................... ..................N................................................i......N..... .............N..............N....................N.......N...........N.......... ..........................N...........N......................................... .........................i...........................N.......................... .......N.......i.....................................................N.......... ..............N.....N..N..N.....N...................... Pos Score Pred ------------------------ 50 0.209 - 62 0.149 - 66 0.155 - 98 0.126 - 129 0.353 - 135 0.675 Yes 146 0.233 - 196 0.190 - 214 0.323 - 258 0.552 Yes 274 0.169 - 363 0.407 - 417 0.447 - 499 0.282 - 548 0.579 Yes 555 0.392 - 574 0.428 - 589 0.417 - 610 0.127 - 618 0.462 - 630 0.247 - 667 0.221 - 679 0.131 - 746 0.515 Yes 774 0.173 - 808 0.371 - 816 0.704 Yes 870 0.059 - 895 0.060 - 901 0.050 - 904 0.044 - 907 0.045 - 913 0.043 -

Explain the output -- Processing locus 874: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAGTATGAGAAGCTTTGTTTATTTTTATAAACACATAATAACAGTTTGAAAAAATTACTTACCTAAATAAAGGTAATAT TCTCGTAATTTACTAAAATAATTCATACAAAATATAAAAACAATTACAAAACTCTTCTTTTTTGGGCTCTTTACATCTTA TTTATACTTTGAAAATTACGATATCCATTTAGAGAAAAATATAACATGCACGTCACAAATTTATTTATTTTTTGAAAAAA ATTACACACAAAGAAAACGACAACTTCGTTCCTTGTTAATTGTTATGATCTCTCCGACCAAACCCAAGCAGCTTCTCCCT CTTAAATACCTCTTCCTTCTTCTAAGATCTCTGGACCATTCTGTACTTTCCCTCAAACCCAAAACACATTTTGTTTTTTG TTTCCCATCTAAAATGCGTATGAGTTGTAACGGATGTCGAGTTCTACGAAAAGGCTGCAGTGAAAACTGTAGCATAAGAC CTTGTCTTCAATGGATCAAATCCGCTGAATCTCAAGCCAACGCCACCGTCTTCCTCGCCAAGTTTTATGGCCGTGCGGGG CTCATGAACCTCCTCAACACCGGTCCTGACCACCTCCGTCCTGCGATATTTAGATCATTGTTGTACGAAGCATGCGGGAG GATTGTGAATCCGATATACGGATCCGTGGGGTTATTGTGGTCAGGAAACTGGCATCTTTGTCAAGCGGCCGTTGAGGCAG TAATGAGAGGCTCACCCGTGACTCCGATCGCATGCGACGCAGCGGTTACTGGTCAAGCACCTCCTTTTAACAACAAGCTT TGCGACATAAGACACGTGTCAAGCAGGGACGAGAACGTTAAGAGACGGAGCCGTGGTGCATGCAAGGAAGAGAGAAACGT GAGGTCTTTGAGTCATGAGTCGTCACTGAGTCACGAGTCACCGGTGTCTTCTGAGGAGACGACGACGGAGGAACCAAAGA CTTGGATCGGGCTTGAGCTGACTTTGGGGTTGGAGCCTTTAGCACGTGGAAATCACGTGGTGGTACCGATGAAGAAAAGA AAGTTAGAGAGGTGTGGCACGTCTGAGGATGAGGACACGTGTAAGATTGAGCTTGGACTGGTGTGCAGTGAGTGAATGGT TCTTTTTTTGTGGCTGGTCTTAATTACAAGTTTTGGTGTTGAGTTTTAGGTGTACAAATAGAGATTAACGAATCTCTCTT TTTTCTCTTTTTGA .....N.......................................................................... ................................................................................ .............................................i.................................. ............................................N................................... ................................................................................ .............N.....N.............N.............................................. ..........N.......................................................N............. ...i...................................................................i........ ................................................................................ ..i............................N................................................ ...........................................................i.................... ..............N................................................................. ....................................................................N........... ............................N..............................................N.... ................................................................................ .............. Pos Score Pred ------------------------ 6 0.173 - 206 0.619 Yes 285 0.151 - 414 0.340 - 420 0.416 - 434 0.424 - 491 0.196 - 547 0.300 - 564 0.616 Yes 632 0.594 Yes 723 0.529 Yes 752 0.378 - 860 0.753 Yes 895 0.158 - 1029 0.128 - 1069 0.123 - 1116 0.039 -

Explain the output -- Processing locus 875: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGTAAACGTCATCGATTTGATATTAATGGGCCTATAAATCTACATATGGGCCGAATGTCAACTCGTCAGGCTATCGGCC CCTTGCTTATGTTGTCCTTAAATGAGTTCTTATAATGCGCCGTCAAAATTAACAATTAAGATGCGCCATAACATAAAATT CAAATCAAAGGGCACATTGAAAATTCGAAACACCGCTCAAATTTCTCTCTGGAAAAAATGTTCAGATTCGATGATCGCTG ATCAGACTTATCTCTTTATCAATCGGGTCCAAGACAGAAGGTTCGATGAAGAATCTCTTCGGATTCTCGAGTTGTCTCTG GTTGCAATGAATGTGAAATCGTTTTTGGAAGTTAGATCTCGATTGAGAGATTTCATGAGATCTGAATCGGTTGTTATATT CGGTGAGCTCACCGGAGAATCCATGGTCGCAAAACTCTCCGTACTTGAATTCTTCGCTCGTGCTTTTGCTCTTCTCGGTG ATATGGAGAGTTGTTTGGCTATGAGGTATGAGGCATTGAATTTGCGGCAGCTCAAGTCTCCTAGCTGTTTGTGGTTAGGG GTTTCACATTCAGAATGGACCAAATTTGCAGTCCAATCTATGGAAAATGGATTCCCTTCCATTGCAGGAAAGGCGTCTGA AAATGCCCTGTTGAGCCTTAAGAAGGATAGTCTTATTGAACCAAAATCAGAAGATAATTCTGACATTTTGGATGCTGCTG AGAAAGTAAGGAGACTAAGGGATTCAGCTGCCTCGTTAACATCTTCACATTCAGGTATTTTCATTTATATCGTGTCTTCT TTAAAGTTTGCTGTTTGTAACAGACTTCTGACTACATTTTGATTGCTTTCTTCGTCTCTATCTAAAGTATTGGTCTTAAA TTACTCGTTTTATTCCCACCGTTCAATGCCTTCAATGTAGTGTGACTGTTATGTATGGGAG ..........................N...................i........i........................ ........N............N............N.........................i................... .........................................................N............i......... .............................................N.................................. ......i...N...........................................N......................... ......................i......................................................... ..N.................N......N.................................................... ..............N........................i......N................................. ..N....................................................................N........ ................................................................................ ................................................................................ .........................N........N...............N...N...... Pos Score Pred ------------------------ 27 0.362 - 47 0.590 Yes 56 0.550 Yes 89 0.356 - 102 0.435 - 115 0.296 - 141 0.540 Yes 218 0.355 - 231 0.742 Yes 286 0.300 - 327 0.505 Yes 331 0.383 - 375 0.263 - 423 0.658 Yes 483 0.411 - 501 0.178 - 508 0.328 - 575 0.161 - 600 0.661 Yes 607 0.251 - 643 0.206 - 712 0.114 - 906 0.059 - 915 0.069 - 931 0.048 - 935 0.065 -

Explain the output -- Processing locus 876: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATCTCCCCAACGAAAACAGAAAGGAATTTTGATTTATTTTTAATTCGTTTTGTATGGATTTTACTTGTATACCCCAACT GAAATCGAATATTTACAATAAAAATTTGGATCTATTCGATCCAGACCCGATCCATTATCCCAAAGAGAATATATTTTATT GGATCTCTCTTCTATTCTTCGGTTTCTTTTTCTTTTGAAAACGAAACTGAAACTTCTCCTTCTCAATTTCAAAAGCTTTT CGATTTATGTTCCTCAAACCTCAAACCGCTCTCGATTTCAATCCTCTCCCTTCTCTACTCAAATTCTCAACAAGAATCCT AATCTCTTCTTTCTCAGAATCTTTTCTTCTCCTTCTTTAACCCTAACCCTAATGCCGCTGAAAACAATGCTGAGAGGAGT AGGAACGAAGGAGAATTCAACGACGGAGGAGCCTCCGCCGGGAACGAAGCCAATCGATGCTGTCACAAAGGCAACGACGG AGCCTCCGATGACGACGGAGGAGCCATCAGCGTCGAAGCAAAATCCGGTCGTAATTGAGGGAAGAGGAGTTGAAGAAGAA CAAATCCCGACCATAATCACGACCGTAGTGGAAGAAGGAGAGAAGTCTGACAACAATGAGGAAGAAAATTCTGAGAAGGA TGAGAAAGAAGAGAGTGAAGAAGAAGAGAGTGAAGAAGAAGAGAAAGAAGAAGAAGAGAAAGAGGAAGAGGAGAAAGAAG AAGAGGGGAATGTTGCCGGAGGAGGATCCTCCGATGATAGTAGTAGAACCTTGGGCAAAGAGTCCTCCTCCGATGAGAAT ATGGATGATGAGACTGCAGTGGGAAAGCAAGTGGATATTCCGGCGGCTATGAAGATCAACGAGATGGGCCAAGAGAATGA TGGGGATCCGAAAGAGAAGGATGGTGATCTAGAGAAAGATGGTGATCAAGAGAAGGACCCGAAAGAGAAGGACCCAAAAG AGAAGGATCCGAAAGAGAAGGACCCAAAGAGAAGGACCCGAATGGTCCTTCAAAACCCGTGGAAGCAAACTTCACAAGAA GGACCACTCAGAGAACCAAGAGTTGAATTTGGTTGTACTTGTTAGGATTTGAATTTGGAATGTTTGGTTTTAGGATTTGA ATGTGGTTTGTTTTGTTTGGATTTGACTCCGGATTTGTTATGTTAGGATTTGAATTTGATAAGT ......................................................N......................... ................................................................................ ................................................................................ ......N......................................................................... ...................................................i..............i............. ........................................................N....................... ........N....................................................................... .......................................................N.......................N ................................................................................ .........N.......................N......................................N....... i...N..N........................................i..............i............N..N ....................N.................N......................................... .........................................N...................................... ...........................................................N.................... N......................................N........................ Pos Score Pred ------------------------ 55 0.372 - 247 0.105 - 372 0.731 Yes 387 0.815 Yes 457 0.243 - 489 0.399 - 616 0.436 - 640 0.230 - 730 0.203 - 754 0.078 - 793 0.079 - 801 0.769 Yes 805 0.126 - 808 0.150 - 849 0.619 Yes 864 0.790 Yes 877 0.273 - 880 0.307 - 901 0.496 - 919 0.204 - 1002 0.283 - 1100 0.150 - 1121 0.067 - 1160 0.031 -

Explain the output -- Processing locus 877: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGATCAAGAATAACGGAATAAATAAACTTCATTAGTAAAAAAAATTGGCAAGAATTTGAACCATAGGTCCATAACCATA ACCATAAATAGTGTGCTTTAGATGATGATTATGAATCAAAGGCCCATTATGATATCCAAGGCCCAAGTGAGTGAGCAATT AAGGCTTCATAAATTTGTCTGCGCCATTCAAGTAGAGTTAGGATTTCAGCTGCTCACGGCTAGCCTGCTACACCTCCCCA ATTTTGTAATAAGAACACACCTTCCCCTGTTTCCGAGAAACCCTAATCTGAAAAACTTTGAGATGGGGAAAAGAGGAAGT AGCAGCGGGAGCGTTCCTATTCGGGGATTTACGAAACTTGTTACAGAATCAAGAAATAAGGGATTAACTGTGGAGGAGAT TGTTGATGATCACCGTTCCAATAACCGTGAATGCGTTCGGCTGAAACGTCAAACCCTCATGATCAAAGTCAAACAAATCA TAAACTCATTGAGCGAAGAAGAAGAGGAAAGTGAAGGAAGTAGGACTAAGAAAAAACAGAGGAGAGATGATTCAGAACAG AGGAATTCGGATTTATGTATATCATCATCGCCTTATTCTGCTTCATCATCCGGAAATGTATTGACATCGGAAGATAACAT GCAGTTTGATGTCACCAATGATGGTTTACGTGTTTCTTACTCTAATAAATCCATGACGCCTTTTTGTGCCGATACAATGA AGAAAATGAAGATGAAAGGTATATCTAGTGAAGAGGGAAGTAAGAATTGTGATGTAGAGGTAAAAGGACCAACCTTTAAG GACTTTGGTGGGTTAACCAGTTTATAGACAATGCTTATACCAATCAATAATCCATCAACCAAAACCTATACTTGAACACA GAGACTTGCTTCCCTAAAACTGAAAACTTTGCATAATCACAATCAA ................................................................................ .....................N..N.....N.................N............................... ................................................................................ ..............................................................i................. ................................................................................ .....N........................i...........................N..................... ..................................................................N............. ..............N........................................N......................i. ........N........N..N...............................i.......................i... .....N.....i.......................................N............................ ..............................N................................................. .............................................. Pos Score Pred ------------------------ 102 0.200 - 105 0.236 - 111 0.186 - 129 0.257 - 303 0.809 Yes 406 0.179 - 431 0.695 Yes 459 0.330 - 547 0.312 - 575 0.234 - 616 0.273 - 639 0.768 Yes 649 0.207 - 658 0.371 - 661 0.377 - 693 0.587 Yes 717 0.698 Yes 726 0.419 - 732 0.659 Yes 772 0.124 - 831 0.162 -

Explain the output -- Processing locus 878: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTGATCTCGAGACAAACAAGAAACTAAAACAGGTTCTTCTTCTCTTCTTTTACCTTTTTGTTTGCTTTCGTCCTGATT CTTGTGACATTTATTGTCTGATGTGAAACTTGTCCTTTATCTATTATGCCTCGATTTTTATTTTTATTTCCCCCTCATAT ATTGAATCTAGATCTGGTTTTGATAATCTTGGAATCAGACAGTGTTTGTATATTTCGGACAAACAAGACGTTAAAAAGCT GCCTTGTCTTACAAGAAACTAGATTTTGTCTCGGGGTCGATCTTATCTCCAAACAGAGAGAAATTAACAGAGACATGGGA GGTGCAGTGACGAAATCTGAAACTCTGCAAAAAGAATGGGTTCCAGAGACAAAGTTGGAGGCTAAGATCATAGAAGCCGT GCAACGCAGGGCATCGAGGGGCACCACAATGAAATCTTTCAACAGTATTGTTCTCAAGTTCCCAAAGATCGATGATGGTC TTCGAAACTGCAAAGCCATTTTCCAAGAATTCGATGAGGATTCAAACGGGTCAATAGATCACACGGAGCTAAAGAATTGT ATCAGGAAGCTAGAGATCTCGTTCGATGAAGAAGAGATTAATGATTTGTTCAAGGCTTGCGATATTAACGAGGATATGGG AATTACATTTACAGAATTCATTGTTCTTCTTTGCCTCGTCTATCTTCTTAAAGACGATTCATCCACTCTCCAAAAGAAAT GGACAATGGGAATGCCAAAGCTGGAACCAACATTCGAGACGCTTGTTGACACATTTGTGTTCCTAGACGAGAACAAGGAC GGATATGTTAGCCGCGAGGAGATGGTCCGAGCCATTGATGAATCAGGAGAACGATCCTCCGGGCGGATTGCAATGAAGAG ATTTGAGGAAATGGACTGGGACAAGAACGGAATGGTGAACTTCAAAGAGTTTCTCTTCGCATTTACGCAGTGGGTTGGGA TAGATGAGAATGAAGAAGAAGAAGAAGAAGACAACAATGAGAAAGCTTAAATGTTATGAGTTTCTAAGTCTAAATATGTG TTTTGTGTTTGCAGAATCTAGTAGTAACAAAAGATATTAATTAGTTGATGTTTATGTTAACGCAAACAT ................................................................................ ....................i........................N.................................. ................................................................................ ..........................................................................i..... ...................................i............................................ ............................i..........................................N..N..... .................................N.............................................. .........................N..............N..................................i.... ..............................................................................i. .....i.....i.................................................................... ....N................i...............N..................................i....... ..........i....................i................................................ ...N.....N..........................N.............N....N...................N.... ...............................................N.....N............... Pos Score Pred ------------------------ 101 0.509 Yes 126 0.391 - 315 0.721 Yes 356 0.821 Yes 429 0.684 Yes 472 0.358 - 475 0.367 - 514 0.135 - 586 0.138 - 601 0.186 - 636 0.556 Yes 719 0.801 Yes 726 0.852 Yes 732 0.900 Yes 805 0.427 - 822 0.580 Yes 838 0.163 - 873 0.509 Yes 891 0.759 Yes 912 0.714 Yes 964 0.304 - 970 0.346 - 997 0.283 - 1011 0.126 - 1016 0.085 - 1036 0.123 - 1088 0.035 - 1094 0.041 -

Explain the output -- Processing locus 879: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAACAAAAGCATAGTACATGTGAGAAACAAAAAGACGAATTTGAACATTAAATCAGTAACATACATACCAAACAACCCT TGTTATGGGTTTGGTTGGTAATCCAAGTATATCTATTTAGTACCAATCCTAAAAGTAAGAAGAAGCAAAACCGGTACTTT CTATCTATATCTTATCATGAACTTCGAATTTATCTTTCTTTACCTATTCAGATTAGTTCGCTATTTCTTGCTGCCCCCAT CCCAAAAAAGAAAAAAAGGTACGTACCGTTTTGTGTCATTAATCGTCACTTGGAATAAGAAAATATTTTGTAAAAGAAAT GAAGTAGAAAATGATCCACCTATAAAAAGATTTGATATAGAAGAGTATAACAACGAAAATATAAAACAAAGTAGAGAGGA ACTAGTAGCAATATCTCTCTTCTATCTTTTCATTTCCTACTCTTTTCTCACTATGGCCAAAGACGGTGGCGTTTCTTGTC TACGAAGGTCGGAGATGATCGGTATAGGAATCGGAGAACTTGAATCTCCGCCGCTAGATTCTGATCAAGTCCACGTTCTC GCCGTTGATGACAGCCTAGTTGATCGAATAGTCATCGAAAGATTGCTTCGAATCACATCCTGCAAAGTCACGGCGGTTGA TAGCGGATGGCGTGCTCTCGAATTCTTAGGATTAGACGACGACAAAGCCGCCGTCGAATTCGATAGATTGAAGGTGGATT TGATCATCACTGATTACTGTATGCCTGGAATGACTGGATACGAGCTTCTGAAAAAGATTAAGGAATCGACAAGTTTTAAG GAAGTTCCTGTTGTAATTATGTCGTCGGAGAATGTAATGACTCGTATCGACAGATGTCTTGAAGAAGGTGCGGAGGATTT TTTACTAAAACCGGTGAAACTCGCCGACGTGAAACGTCTGAGGAGTTACTTGACGAGAGACGTTAAAGTCGCCGCCGAAG GAAACAAACGGAAGCTGACCACTCCACCGCCGCCGCCTCCGCTCTCAGCCACATCCTCGATGGAATCTTCGGACTCTACG GTGGAATCGCCTCTGTCTATGGTTGACGATGAAGATTCGTTGACAATGTCGCCGGAGTCTGCAACTTCGCTTGTGGATTC GCCGATGAGGAGTCCCGGATTAGCTTAGAGAGATCCGTGTGATAACGATCTCCGTTAACTTTTTACTTTTGACGGTTTTA TTGACTGCAACGTTGACTTGAACGCCGTCAGTTTATACTTATAGATC ..................N............................................................. ....N........................................................................... ................N............................................................... ..............................................................................N. ..........N..................................................................... ....................................................i........................... ..............i................................................................. .......N........................................................................ ......N......................................................................... ....................N........i.................................................. ..................i............N....i................i.......................... ................................................................................ ...........................................................i.................... ..................i.........N................i.................................. ....i........................................................................... ............................................... Pos Score Pred ------------------------ 19 0.483 - 85 0.283 - 177 0.333 - 319 0.221 - 331 0.284 - 453 0.726 Yes 495 0.721 Yes 568 0.339 - 647 0.393 - 741 0.455 - 750 0.699 Yes 819 0.757 Yes 832 0.169 - 837 0.610 Yes 854 0.771 Yes 1020 0.564 Yes 1059 0.585 Yes 1069 0.305 - 1086 0.684 Yes 1125 0.650 Yes

Explain the output -- Processing locus 880: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTGATTAATAGAAAGAATGAGAATCATTTACTGCTTACAGCTCATAGAGACTATGAAGATTCCAATTATTACAAGTGAG ATAGAGACGTACATAAGTTGAATTGTATTGTGCATTTTAGCATATAACCAAAGCATTCCACATGAAAGATATAAATATAA TTACATATTTGCATTAATGAATATTAACAAAACCTATCTCTCTGTCTATATATACACACACACACACACACATATATCTC CTATCATTCATCGTACACACACACTCTCTATGACAAAGCTCCCCAACATGACGACAACACTCAACCATCTATTTGATCTG CCGGGGCAGATTTGCCATGTCCAGTGTGGTTTTTGCACCACTATTTTGCTGGTGAGTGTACCGTTTACAAGCTTGTCAAT GGTGGTGACTGTGAGATGTGGGCATTGCACAAGCCTTCTCTCTGTCAATTTGATGAAGGCTTCCTTCATTCCTCTCCATC TCCTTGCTTCTCTCTCCCATCTTGATGAGACCGGGAAAGAGGAGGTTGCAGCTACAGATGGTGTGGAAGAAGAAGCATGG AAGGTGAATCAGGAGAAGGAGAACAGTCCAACGACTTTGGTTTCATCTTCAGACAATGAAGATGAAGATGTGTCTCGTGT TTACCAAGTTGTCAATAAACCACCTGAGAAGCGACAAAGAGCTCCTTCAGCTTACAATTGCTTCATCAAGGAAGAGATCA GGAGGTTAAAGGCTCAGAATCCAAGCATGGCTCACAAGGAAGCTTTCAGCTTAGCTGCCAAAAATTGGGCCCATTTTCCT CCAGCTCACAACAAGAGAGCTGCTTCAGATCAATGTTTTTGTGAGGAAGATAACAATGCGATACTACCATGCAATGTTTT TGAGGACCATGAAGAAAGCAATAATGGGTTCCGAGAGAGAAAGGCTCAGAGGCATTCCATTTGGGGAAAATCTCCATTTG AGTAATAACAATTTGGGATATGAAAATTTAACAAAAAAATAAATAATGGCGTATGACATGTGATGGTGACTCTTTTCTTC TAGGGTTTGATTATGTTGGTTTAG .................N...................................N.......................... .............................................................N.................. ................i............................................................... .............................i.................N................................ ................N.............................................................N. ...............N....................................i........................... ........................N................................N..................N... .......................................................N.....i.....i............ ................................................................................ ..........................i..................................................... ................................N......................i............N....N...... ........N..............i........................................................ ...................N.........................N......N....N....N................. ............N........... Pos Score Pred ------------------------ 18 0.259 - 54 0.332 - 142 0.349 - 177 0.745 Yes 270 0.525 Yes 288 0.435 - 337 0.484 - 399 0.366 - 416 0.485 - 453 0.746 Yes 505 0.114 - 538 0.144 - 557 0.286 - 616 0.427 - 622 0.631 Yes 628 0.526 Yes 747 0.785 Yes 833 0.438 - 856 0.598 Yes 869 0.247 - 874 0.242 - 889 0.331 - 904 0.731 Yes 980 0.095 - 1006 0.111 - 1013 0.089 - 1018 0.062 - 1023 0.056 - 1053 0.041 -

Explain the output -- Processing locus 881: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAGGTTTCAAAGTTCAAAACTATCACAACTAGTCTTGATCAATTCTATATAAGATCTGTGATCTTGGTTGAAGAAAAGAA TCGTCGTAGGTTGATATTTAACAAGGAATGGCAAAGGAAGGGGGCTTGGGAGAGAACTCAAGATGGAGTCTTGGAGGCAT GACCGCTCTTGTCACTGGTGGCTCTAAAGGCATCGGGTCAGTCCCCATTGTGAATCTTGACTCCCATTTTCTATGCTCTT TTATTGTATTGTCATGTTTAGTTATGGAAGAACTAACTCTGTTTGTGTGTGTCATGAATAGGGAAGCTGTGGTGGAGGAA CTAGCCATGTTGGGAGCAAAAGTCCACACGTGTGCCAGAGACGAAACTCAGCTTCAAGAACGCTTACGTGAATGGCAAGC AAAAGGGTTTCAGGTCACCACTTCTGTCTGCGACGTCTCTTCTCGTGACCAACGAGTGAAACTCATGGAAACTGTTTCCT CTCTCTACCAAGGAAAACTCAACATCCTCGTCAACAATGTGGGAACGTCAATATTCAAGCCGACCACAGAGTATACAGCA GAAGATTTCTCTTTTGTAATGGCTACAAATCTCGAGTCAGCTTTCCATCTCTCACAGCTTGCCCACCCATTGTTAAAAGC CTCTGGCTCAGGGAGCATCGTGCTCATATCCTCTGCTGCTGGAGTCGTGCATGTCAATGTTGGATCCATCTATGGAGCAA CCAAAGGAGCCATGAATCAGCTGGCTAGAAACTTAGCTTGCGAATGGGCGAGCGACAACATAAGGACGAACTCTGTTTGT CCTTGGTATATCACAACTCCTTTAAGTAACGATTTTTTCGATGAAGAGTTTAAGAAAGAAGCGGTGCGTACGACACCAAT GGGGCGTGTTGGAGAAGCAAATGAAGTCTCACCGCTTGTGGCATTTCTTTGTCTTCCTTCAGCTTCTTATATTACTGGTC AGACCATTTGCGTTGACGGCGGTGCCACTGTTAACGGTTTTTCCTTCAAGACTATGCCATAAAACACATATGCATGTGTT TGGATCATATTTTCAATAATTTAAACTTACGGGTTATATACATCGTCAATATGTAATAAATGTAATTTAGATGTGTCCAC ................................................................................ ...........................i..................................N...............N. ........................................................................i....... .............i.........i.............................N.......................... ......N................................................................N........ ................................................................i............... ....................................N........................................... ..................i............................................................. ..................................................N.....N..............N........ ...........i...............................N.................................... ........................................N.....................................i. ....................N........................................................... .....................................................N...............N...N...... ..................................................N........N..........N......... Pos Score Pred ------------------------ 108 0.742 Yes 143 0.184 - 159 0.285 - 233 0.658 Yes 254 0.572 Yes 264 0.867 Yes 294 0.326 - 327 0.450 - 392 0.224 - 465 0.716 Yes 517 0.478 - 579 0.645 Yes 691 0.355 - 697 0.333 - 712 0.352 - 732 0.535 Yes 764 0.391 - 841 0.182 - 879 0.850 Yes 901 0.192 - 1014 0.466 - 1030 0.113 - 1034 0.203 - 1091 0.041 - 1100 0.033 - 1111 0.045 -

Explain the output -- Processing locus 882: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCACATGCTACACGAGCTCTTTTAGTCTAGCATTAATGTTCTGTTCATTTTGCTGTCTTGAAAGAGAATTTATATTTCA TTAATATTTTACCAAAATTAAATCACATATATATATTTGTCTTTCGATATGTGGACCGGACAAACACAAAGTAGCGTCCT TATAATTTGCTATCTCTCTCATCCTATTTCAAGTCTTTCTCTATCTACTTTCCTCAGAAACCCATCCTCAGAGATGGCAG ACTTGAGTTTTTATGTCGGAGTCATAGGGAATGTTATATCGGTGCTTGTCTTCCTCTCCCCTGTGGAGACGTTTTGGAGG ATAGTGCAGCGGAGATCGACGGAGGAATACGAGTGTTTTCCGTACATTTGCACGTTAATGAGCTCGTCGTTATGGACATA TTACGGAATAGTGACACCTGGTGAATACTTGGTTTCTACTGTCAATGGCTTTGGTGCTCTTGCTGAATCCATCTACGTTC TCATTTTCCTCTTCTTTGTCCCCAAATCCAGATTCTTGAAAACAGTTGTTGTGGTTCTAGCTTTGAACGTGTGTTTCCCA GTTATCGCGATTGCGGGAACAAGAACTCTGTTTGGAGATGCAAACTCGCGTTCTAGTTCAATGGGTTTCATATGTGCTAC TCTCAACATTATCATGTATGGTTCTCCTCTTTCAGCTATTAAAACGGTTGTGACGACGAGAAGTGTGCAGTTTATGCCAT TTTGGTTGTCATTTTTCCTCTTTCTGAACGGCGCGATTTGGGGTGTCTACGCTTTACTCCTACACGATATGTTTCTACTA GTGCCAAATGGAATGGGATTCTTTCTTGGGATAATGCAACTCCTAATTTATGCCTATTACAGAAATGCCGAACCAATTGT AGAAGATGAAGAAGGCCTCATACCAAATCAACCTCTCCTCGCTTAACCTCGTCTTTTACCCATCAAAATTTCTAAAATAA TTTATCCTGACAAACAAAATAAATATTCGATTCTTTTTTCGGTTGAGTATTGGTTTTTGACTGTG .....N..............................N........................................... ................................................N............................... .........................................................................i...... ............N.................N................................................. .........................................................N.............N........ ............................................N................................... ................................................................................ .....................................N......................i..........i........ .............N...N.......................................................N...... ....................................................................N........... .......N....i....................N...............N..............N............... .....N.......................................................................... ................................................................. Pos Score Pred ------------------------ 6 0.186 - 37 0.230 - 129 0.495 - 234 0.823 Yes 253 0.426 - 271 0.253 - 378 0.256 - 392 0.217 - 445 0.188 - 598 0.151 - 621 0.730 Yes 632 0.539 Yes 654 0.453 - 658 0.113 - 714 0.155 - 789 0.188 - 808 0.441 - 813 0.692 Yes 834 0.387 - 850 0.232 - 865 0.310 - 886 0.117 -

Explain the output -- Processing locus 883: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTATTTAAATTGTTACTACATGAACATGATATAGAACTATTTTTATAACGATATAATACACACGAAGGTTAGTAAGATTA ACAAACATTTAAATAAAGTAATCATTAAAAGAAACGACGATGAAGGTACCTAATCGTTACATTAGAAGGCTTGTATATAA AACCTCAACAAAACCTCTCCCTTTCACTTCATCACTTCAAGTCTTCTCTTCCTCGTTGATCAAACATTTTCAAGTACTTT CCGGCGAAACATCAATGGCCGTTTCGAATTCTTTAACAATCTCTCCGAGGAAACTCCGGTCTGATCTTTACTCATACTCA TACCAAAACAATTCCAAGACACCTCTGGTTATCTCTGTTCTATCGTCACTGATTGATCGAACTCTGACTCGAAACGAGAG GATTAGCCGGAGAGCTTTGCCTTCCTCCGGCGCCGGTGGCAAAACTCAGATCTTTGATTGCCGAGAAATCCCTGATATGA CCATCCAGTCATACCTAGGGAGAATTTTCCGGTACACTAAAGCCGGTCCTTCGGTTTACGTGGTGGCTTATGTCTACATT GACCGGTTCTGTCAAACCAATCCCGGTTTTAGAATCAGTCTCACTAATGTACATCGTCTACTCATCACTACCATCATGAT CGCTTCTAAATACGTCGAAGATTTGAACTATAGGAATTCATATTTTGCGAAAGTAGGTGGACTAGAGACAGAGGATTTGA ATAAGTTAGAGTTAGAGTTTCTGTTCTTGATGGGATTTAAGCTACATGTGAATGTGAGTGTGTTCGAGAGTTATTGTTGT CATCTTGAAAGAGAGGTTAGCTTTGGAGGAGGTTATCAGATCGAGAAGGCATTGCGTTGCGCTGAGGAAATCAAATCCAG ACAAATGATCATTCAAGATCCTAAACATCATCATCATCATCATCAGCTTGCTCGAATCTTGTTGTAGAGAAAACAAACTC TGTTTTGTTTTTAGTCTTAGTTTTTTTCTTTTGTCGTTTGTGCATTGTGGTCTTGACTTTCTGACAAACTTGTTGGTTAG AGTTTC ...................i.....i...................................................... .......................................N........................................ ................................................................................ ..............i................................................................. ................................................................................ ............................................................................i... .....................................................................N.......... ..............................................N............................i.... ................................................................................ .............................N...............i.....N............................ ................................................................................ ....N........................................................................... ................................................................................ ...... Pos Score Pred ------------------------ 20 0.511 Yes 26 0.668 Yes 120 0.245 - 255 0.569 Yes 477 0.624 Yes 550 0.281 - 607 0.153 - 636 0.511 Yes 750 0.271 - 766 0.503 Yes 772 0.486 - 885 0.210 -

Explain the output -- Processing locus 884: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGCAAACAAAAAGACCATAAAGTAGCAAAAACAAACATTTTGGGCCAAACTGATAAAGAAAAATAGTTTGGGGATACAA ATAAGAAATACTAAAATATAAAAAAGCTGTGGGGAACGTTGCAAAATAAAATTCCCCAAAAACACGCGCACGATAATGAT TCCCTTTACTCTTGTTGCGTGCAATTTCCGCTATTATGCGCATTCGTCATCACTTTCCCATTCCTCTCCGATCAGTTCTT CTCTCTCTCACACTGCTTTTGCCTCCATTGAACCCTTCTTCTCTCTGCTCACCGGAATATTTCTCTCACAAAAGATGAAG CAATTCTGGAGTCCAAGTAGTATCAACAAGAACAAAGCTATGGTTGAGAATCTGCAAAATCATGGTATAGTTACTTCAGA TGAAGTTGCTAAAGCTATGGAGGCTGTGGATAGAGGCGTATTTGTAACAGATCGTTCCTCTGCTTATGTTGATAGTCCCA TGTCTATTGGTTATAATGTGACTATATCAGCTCCTCATATGCATGCAATGTGTCTTCAACTTCTTGAAAAACATTTGAAA CCGGGAATGCGTGTACTTGATGTTGGCTCGGGGACCGGGTACTTAACTGCTTGCTTTGCGGTTATGGTTGGAACCGAAGG GCGTGCTATTGGTGTGGAACATATTCCTGAATTGGTGGCTTCTTCTGTTAAGAATATTGAAGCGAGTGCTGCTTCGCCAT TTCTGAAAGAAAGGTCTCTTGCTGTTCATGTTGGTGATGGGAGGCAAGGATGGGCTGAGTTTGCTCCGTATGATGCTATT CACGTCGGAGCAGCAGCACCGGAGATCCCAGAGGCGTTGATTGATCAGTTGAAGCCCGGTGGGAGACTGGTGATTCCGGT TGGGAACATATTCCAAGATCTGCAAGTGGTGGATAAGAACTCAGATGGTTCTGTTAGCATCAAGGATGAAACTTCAGTTC GTTATGTTCCTTTGACTAGCCGTGAAGCCCAGCTGAGAGGGGACTGATGAATATGCTCGTTGTACAGATGAATGAATCAA GTAAGCTCAGTCGCCTTTCTAAATTAGCATAGTTAGTTAAGAACTCAAATCACTGTGAGTAAAACT ................................................................................ ...........................................................................N.... ...................................N............................................ ..........................................................................i..... .......................................i.....................N.................N ................i................................................N.............N ...............N......................i...N....N................................ ......i............N...........................................i................ ................................................................................ ...........................N........N............i...................N..N....... ................................................................................ ............................................i....................i.............. ...N..........................................N.....N..............N...N........ .................................................................. Pos Score Pred ------------------------ 156 0.248 - 196 0.313 - 315 0.845 Yes 360 0.846 Yes 382 0.076 - 400 0.136 - 417 0.755 Yes 466 0.140 - 480 0.313 - 496 0.160 - 519 0.568 Yes 523 0.227 - 528 0.364 - 567 0.815 Yes 580 0.196 - 624 0.528 Yes 748 0.234 - 757 0.399 - 770 0.642 Yes 790 0.083 - 793 0.080 - 925 0.562 Yes 946 0.592 Yes 964 0.104 - 1007 0.294 - 1013 0.179 - 1028 0.098 - 1032 0.117 -

Explain the output -- Processing locus 885: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 886: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCAGCAAAGTCATTGATTCAACTACAAGAGAGATGACCAGAGGACTAACTTCTGCTTGTGAGCAGGTATATTATAAAACT TTACCAATTGTGTAACCTTAAACTTTTGACTGTTGCTTAGATGTTTTCATTATTATTATTCAGGATCTTCATAAACTTGG AGAATACCTTCCATTACTCTTTAACTTGGTGCATTACACTGATAAGATTAAACGGGTTTCGAATCTTAAGATAAGATGGA GTAGTGGTTTGATCTCTCAGACCTTGATTCAGCGTACATGTCCGAAATTCTTCCAAGTCGATAACATAATGTTCGAGTTT GGAATGGTTCTTTTCATTTACGCGGTTAAGCTCCGCGAGAGAGCAATGGAACTAGTATCCACAGATTCTAAGAAAGCGGT AGCAGTTTACCGAGAAGCATCTGGAGTTTTCCATCATCTGTCTCATGAGATTCTTCCTTTATTACAAACTTGTCTGCCTC CAGGGAAGCTTCCGGAACTAACTCCTCCGCTTTGCTCTGCTTTGAGTCTCCTCTGTTTAGCTGAAGGTCAGGCTGTAACT ACAGAGAAGGCTGAAGAAAGCGGCAAAAGTGCAAGCCTTTTGTCGAAACTGCACTTTGGAATTTTTCAATTTCTTTCTGA AGCCTATGCTCTTTTGTCATCTAGACTAACAGGAGAATATAAAGACCTATCGACCCGCTTTCTCGAGTATGTAACAACAA TGGGAGCTTTACACGAACTAAAGAGCCAAAAATACTTAGCGGAATTGCTAGAATCCGAAGATCGAGTAGGCGATGCGGTA GGGGTTCTCCGGCGTGCCTTAGCAGCGGCGAAGAAGAGTACGCCGTCCAAAGATGACAAGTGGATAGCAATATTCAAGAA GGAGAGAGAGGATGTAGCTAAAAACATGGCTAAGTATGAGAAGCTAAACGATTCTATGATGTTACAGAAGATTCCAATTG ATAGAGAGATACCTTTTCCAAAAGGTGAGAAGATTGTTAATCTCATTCCTTATACTCCCACAAGAGTGGTACGAGAACTT CGATTTAAGTCTTAGAAGAAGATCTAATAACTGTATGATTAATTCTTTTGTTTAGTTCTTTTGAGTTTGGAGAAGAGATT TAAGGTGTCTGATATGTGATACAATTATTTGCCA ................................i............................................... ........................................N....................................... ...........................................................................i.... .....................................i..............................i........... ...i.........................................N.................................. ............................................N................................... ................................................................................ ................................................................................ .....N..............................................................N..........i ........................................................................N....... ....................................................N........................... ...........N.............i.........N...................N..i..................... ................................................................................ ..................................N............................................. .............N.................... Pos Score Pred ------------------------ 33 0.511 Yes 121 0.210 - 236 0.585 Yes 278 0.627 Yes 309 0.690 Yes 324 0.630 Yes 366 0.413 - 445 0.076 - 646 0.162 - 709 0.312 - 720 0.776 Yes 793 0.279 - 853 0.130 - 892 0.158 - 906 0.729 Yes 916 0.211 - 936 0.500 - 939 0.515 Yes 1075 0.086 - 1134 0.046 -

Explain the output -- Processing locus 887: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCCACGTGGAAGAATATGAGTGGTCACGATATTATAAAATGCGTCAAGGTAAACAACAAATAACAAACTCGTTTTCGAG CAAAAAAACTTTGAAGATTGATTTGAAGAAGAAAGCTCTCTCTCAGGTTCTTGTTCTCAACCAGACATTGATTTCTTCGA TTTGCATATTTCCCTTCTTCGTCTTACGAATTTTACTTCCTTTAGCGATGGATTTGATACTACGTTGACTTTTATCTTCC TTTTGGAAATGGCAGCGTCGTCTTCTCTTCTTCTTCTTCTTCACTGTCCCACCTGCAATTTCTACTTTGACTCTTCCTCG TACCTTAAAAGATCGAGACTTTCGAGCTATAGTTCGATTATCTCTCGTGGGTCTCCTTTGTTTGTCTCTAGCTTTGGATC TATGACCGTTAAAAGATTCTCTAGCAGAGTCGGAAGCAGGAGCAATGACGGTAACGAACAATTTGGAGCGTTGGAGCAAG AATCTTTCATCAACAACTCATCGGAGATACGGAAAGACTTGGTCACCGGCGGTGGGATTGAAGCCATTGTTAATCGTCTG AGCAAATGGGTTGTGTCTGTTCTATTTGGTTCAATCATTCTTTTGCGACATGATGGTGCAGCCTTGTGGGCAGTCATTGG ATCGATTTCGAATTCTGCTCTCTCTGTAGTTCTTAAACGTATACTTAACCAAGAGAGGCCTACTACAACATTGCGATCTG ATCCCGGGATGCCATCTTCTCATGCTCAATCCATTTCTTTCATATCTGTCTTTGCTGTTTTATCCGTTATGGAATGGCTT GGAACCAACGGTGTGTCTTTGTTTCTTAGCGGCTTAATCCTCGCATTGGGTTCATACTTTATACGGTTAAGGGTTTCTCA AAAACTGCACACAAGCAGTCAAGTGGTGGTGGGTGCCATTGTTGGTTCTCTTTTCTGCATCTTATGGTACACAATGTGGA ATTCGCTTCTTCGTGAAGCCTTCGAGGCATCTCTATTAGTGCAAATATCTGTATTTCTGTTTGCAGCTACATTTGCGCTA GCTTTCGCAGCGTACGTTGTACTTAACTGGTTCAAAGACGAGAGATAAGCAATACCAAGCTTTACTTATAAAGCTTAAAA TTGTTATCCCTCAAATTCTTTTCAAAATATCGAAATGAAGGATTCAGAGCCCGTTTGTTAAAATCAA ................i......................N........................................ ................................................................................ ...............................................N................................ ........i....................................................................... ................................................................................ ................................................................................ .....N...........................................N..N........................... ................................................................................ ........N............N..............................................N....i...... ................................................................................ ...............................................................N.........i...... ................................................................................ ................................................................................ ..................................N................................ Pos Score Pred ------------------------ 17 0.638 Yes 40 0.385 - 208 0.222 - 249 0.544 Yes 402 0.604 Yes 445 0.734 Yes 566 0.470 - 610 0.272 - 613 0.136 - 729 0.457 - 742 0.208 - 789 0.338 - 794 0.735 Yes 944 0.332 - 954 0.766 Yes 1155 0.125 -

Explain the output -- Processing locus 888: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTGACTTGGATTAAAGTTCCCAAAATATTATAATCTGAATGCAATTTCTTGTATTAATTACAACGATAGTCAATAGAGG CCCAGAAGAAGGTTCAAATAAAGGCCCATTATGGCCCAAGTTATAACCCGAATAAAGGATCCGATCGAGAAGGAGAGAGA CAACAGTCACAGACACAGAGAGATGGCGAGAGAGAAGAAGACGTGTGTAGAGTGTGGGCACAAGGTTAAATCTTTGTTCA TACAATACTCTCCTGGAAATTTTCGTCTCATGAAATGCGAGAATTGCGAAGAAGTAGCAGACGAATATGTCGAGTGTGAG CTATTGATTATTTTCATCGACTTGATTCTTCACAAAACAAAGGCTTATAGACACTTACTCTACAATGTTGTTAATCAAGA ATCTGCAAATGTTCAGCATCTATTATGGAAATTGGTCTTGGCTTATCTTCTTCTAGATACTTATAGAAGCTTGCTACTGA GAAGAACCAATGACGGATCAAATGTGTCTATGAGCTTTCTTTTTGAATCTCTAGAGGTTCTGGTCAATGTACTATCTGCG AATTTCGCATTTGTTTTCTCATTTGCATTTGCTGCTAAGTTGATGCTGGTTATGCCAAGAGGGAAAGAGATTCTTTTAAC GATCTTAATCTCGAGTTACGTCAAGATCTTTCTATTTGCTATGCCGGTCTGGGAATTCCCAGTTTCTGTGATCTTCATCG TAGATATGCTCGTTTTAACCTCAAACGCAGTTGCTCTTAAAGTGATGACTGAATCAGCAACGAGCAGATGCTTAGCAGTG TGTTTCATTGCACACTCAATTAGATTCTTGGTGGATCAGATCTCTGGACACTTAGGATCTGTCATGTAATCTTCTCTTGC AAGGAACTTGTGATCTCATAATATTTTTTTTAGGGTCGTTAAACTCTCTTGTCTAAATCACTTGTGATAATATGTTTAGA AAAATAAA .......................................N........................................ ..............................i................................................. ......................i......................................................... .............................N....N...............................N............. ................................................................N............... ........N...............N....................................................... .........N...........N.......N....................................N............. ..........................................N........N............................ ........................................i....................................... .....N......................................N......................N............ ...............................................................N................ .......................................................................N........ ........ Pos Score Pred ------------------------ 40 0.456 - 111 0.609 Yes 183 0.523 Yes 270 0.143 - 275 0.400 - 307 0.267 - 385 0.429 - 409 0.253 - 425 0.292 - 490 0.334 - 502 0.214 - 510 0.362 - 547 0.075 - 603 0.236 - 612 0.235 - 681 0.717 Yes 726 0.310 - 765 0.257 - 788 0.406 - 864 0.145 - 952 0.046 -

Explain the output -- Processing locus 889: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACAACACTCTCTATGACTCAAGTCAACTGTCAAAGCTTGGTTCCCTGGGTCCTACATAACAAAATCTTCGAATCATATT CTTCCACGTGGCAAGATCTAACCAATGAGAATCTATTTTTCCATATGCTCTCCACACTCCCACCAAACAAATCCCAAGAA TCAACAAAAGAAAAAACATTCACCTTCTCTTACGCGACTCTCGCCGGAAACAGATCGCTCCGCCGTGAACTCGGAAGCCA TGGGGTCACTTTTGCAGGGTTTTACTAAATCACTCGCCATGACTTTCCTCTCCGAGATTGGTGACAAAACGTTCTTCGCT GCTGCTATTTTGGCGATGCGGTATCCTAGGAGGCTTGTATTAGCTGGTTGTTTATCAGCTTTGATTGTCATGACTATATT ATCTGCTACACTTGGTTGGGCTGCTCCAAATCTGATCTCTCGGAAATGGACTCATCATATAACAACATTCTTGTTCTTTG GCTTTGGGTTATGGTCTTTGTGGGATGGTTTTAAAGAAGGAGGAGGTTCTGAAGAATTGGCAGAAGTTGAAGCAGAACTG GATTCTGATTTGAAGAAGACTAATGATCAATCGAAAAACAGTAAGATTGAAGATGAACAGAAAAAGCAGAAAAGACCATT CCTTACTGCATTCTTCTCTCCCATTTTTCTCAAGGCGTTTTCGATTAACTTCTTTGGTGAATGGGGTGACAAGAGTCAGC TTGCGACCATTGGTTTGGCTGCAGATGAAAATCCACTCGGCGTGGTCCTTGGCGGAATTGTGGCACAAACATTGTGTACC ACAGCTGCTGTGCTTGGTGGCAAGAGCTTAGCATCTCAAATATCCGAACGAATTGTGGCTCTTTCCGGTGGAATGCTTTT CATTATCTTCGGCATTCAGTCGCTTCTTACTCCGGTTGATGCTTGATACATCTATATGGTATCCCATCAATGTCTTGAAA CTGCAGGTTTCTTGGGTTTGAATGCTTTTTTGGGCGAGATGAAACCGAAACACGTCACTCCCTTA .............N.................................................................. ........................N...................i................................... ...............................................................................i ......................................i......................................... ...............N.....................................................N.......... .............................................N.................................. ..........N.............N....................................................... ......................N.............................N........................... ............................................................N................... ........................N....................................................... ........................................................................N....... ......................................N................N.............N.......... .....................N................N.......................... Pos Score Pred ------------------------ 14 0.495 - 105 0.343 - 125 0.659 Yes 240 0.664 Yes 279 0.555 Yes 336 0.266 - 390 0.141 - 446 0.487 - 491 0.240 - 505 0.220 - 583 0.109 - 613 0.329 - 701 0.377 - 745 0.154 - 873 0.325 - 919 0.429 - 936 0.231 - 950 0.290 - 982 0.044 - 999 0.054 -

Explain the output -- Processing locus 890: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCTCTACGACTTCTGTGTACTCAACGTTGAGAACACTGATGAGATAAGTTTACAAATACTTCTTTTTATGATGGGTGTT CTTGTTTTTTTCCTTCTATGATTATCTCTCTTTTCTATGCTTTTAAATTGCTACTTTTCTTTTGCTTTTTTGCAGAAGGC TGGATTGATGCGAGAGGATGCAGCACGAGATCGCATGAGAGGATTTGGGTTTCATGAGCATGTTATCAGTGAGAGTATTA AGGAACTGTTAGAGGTCTACGAAGACCAATGGTTTTTGATTGAAGATGATAGCTATGGTCCCCTCCTTGCTATCTGCCTT GAAAAGCAAGAGGAAAAAGAGAAACAATTGGCTGAAGTACAAGCCAACCAATTGCCTGAGAATCACAATGAAGAGATGGC TGAGGAAGAACAACCCAATCAATTGGCTGTGGAAATACACGAAGAAGAGAAGGAGCAGGAGCAGGACATGCATATTGAGT ATGGAAGAGACCAAGCTGATGAGTTATCAATCAATAGCGAAGCAGTCAATGACCCATCACCAGCAGCTATTCATTTGGGA GAAGCATCATCAGATTATGCACAAAGTTCTGTTCGAGGTGTGGAGAATCTTCATTGTGGATGGCTAATTGATGAGGAGGA GACGGATCCTGAGGAGGAAACGGATCCAGAGGAGAAGGAGACCGAGCCAGAGGATCCAAAGGAGAAGGAGACGGAGCCAG AGGAGGATAGTGATGGTGATGATGATGAGATCATTCAGCTTACTCCAGAACCTCTTTGTGAAGAACTTCAAGAGCTTCTC AGGGAAGTACGTGGACAGAAGAGGAGGAAGAAAGAGATGAGGTGGGACAAGTAAAGGAGCTATGGAGCTCCTGTAAGTAG CAAATTGATATATATGTTAGGAAGCCAAATAGTAGTAGTAAGCTATAGTATTGTGGTGAAAAAAGGTAAAACG .......................................N............................N..N........ .................N..................N........................................... .......i.........N................N..................N.....N.................... ............................N................N........N......................... ...................................................................N.......i.... ...................................................................i............ N.................N.............................N............................... ................N..........................................i..........N......... ................................................................................ ............N.....N..N..N....................................................... ....................................N........................N.................. .............N........................................................... Pos Score Pred ------------------------ 40 0.089 - 69 0.147 - 72 0.326 - 98 0.368 - 117 0.318 - 168 0.776 Yes 178 0.256 - 195 0.182 - 214 0.069 - 220 0.159 - 269 0.347 - 286 0.156 - 295 0.246 - 388 0.321 - 396 0.888 Yes 468 0.704 Yes 481 0.460 - 499 0.214 - 529 0.148 - 577 0.264 - 620 0.850 Yes 631 0.274 - 733 0.450 - 739 0.100 - 742 0.152 - 745 0.235 - 837 0.271 - 862 0.092 - 894 0.050 -

Explain the output -- Processing locus 891: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGATTGACACTAGGATCCGAAACCTTGGACTCCAGTTATATTTCCATTTTTATGCACAATTGTATTAATTTGTAACTATG AATTACATAGACGAATAGTAAATTCATAAGAGATGTGTCAATCAAGCCACAAAAGGGGTTTATCAGTCAATGTACCGGCC TAACCACTCCCATAACCAAAACCACATATCCAAACGTGGTTTTTCATTTGGTACAAAAACCCACACCTTAATTTCACAGA CGAAATTTTGGTTGATAAAAAGTAACCATCTTAACCAGTCTCTCATTTACCCCCACCAGATATACATATGCTTATTTAAA CCGGATGTAACACAACTCTTCTTCACATGGACCTCAAGTAAAATGGAAGCAAACACTTTGTTTCTTCTAAAAGCTCTTTG TCTTCTTTGTTTTAATGTATGCTTCACATTAGCTTCCGACCCTGATCCCATCCAAGACTTCTGCATACCAAAACCTGTGA CATCTCCTTACCATGACCATCACTTCTCCACCAATCTCCCTTGCAAAAACTCCTCCGAAGTAACCACCGAAGATTTTGTC TTTTCCGGTCTAAAAACTGCCGGAAACTTCACGGAGACGGGGTTCGCCACCGTCCCAGTAGGCCCAGAAAACTTCCCCGG CCTTAATACATTAGGCATCTCGTTTGTCCGGGCTGATCTCAAACCCGGATCCATTAACCCACCACATTACCATCCGCGGG CGACGGAAGTAGCCCACTTGGTTAAAGGACGGGTTTACTCTGGTTTTGTGGACTCGAACAATAAGGTTTACGCTAAAGTG ATGGAAGAAGGAGAAATGATGGTTTACCCAAAAGGGCTTGTACATTTTCAGATGAACGTCGGAGATGTTACGGCCACGAT TGTCGGCGGACTTAATAGCCAAAACCCTGGTATTCAGAAGATACCGTCCGTGGTTTTTGGGTCAGGGATCAATGAGGAGT TGCTTATGAAAGCTTTTGGGTTGAGTCTTAAGCAGATTGGTACGTTGAAGAAAAGATTTGATCCTGTCATGTCTAATGAA CATTGATGTTTTCTCTTAAAGTCTTATCGAGATTATTACATCGAAGTTATCTTTTATGTGATGGTAGTTCTTGATCATAA TGTTATTTATATATTTTGACCCAAA ...................................................N.........................i.. ................................N....................................N.......... ................................................................................ ...................................................................N............ ....N.....................N...............i..................................... ..............i...N............................................................. ............N................................................................... ................................................................................ ................................................................................ ................................................................................ i..............i..i................................i............N............... .......................................................................N........ .....N..............................................................N......N.... .....N.................................................N....N..................N ......................... Pos Score Pred ------------------------ 52 0.093 - 78 0.502 Yes 113 0.461 - 150 0.275 - 308 0.368 - 325 0.181 - 347 0.194 - 363 0.683 Yes 415 0.632 Yes 419 0.249 - 493 0.411 - 801 0.856 Yes 816 0.843 Yes 819 0.871 Yes 852 0.659 Yes 865 0.161 - 952 0.092 - 966 0.327 - 1029 0.247 - 1036 0.058 - 1046 0.185 - 1096 0.058 - 1101 0.080 - 1120 0.043 -

Explain the output -- Processing locus 892: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGAAGTGTAGAAACAAAGAGATTATAGCCGTTGGATTTAATAAATGTACGGTTAGGATCATATGCCGGTTAAAGAATGG GTCTATTGGGCCTTTTAATGAAGCCCACAATATATTCACGGTGACATGCTCCGGTCCCAAGGTAGAGAGAAGTTGCGATT GATTTCGGTGAAAGAATTAAGAATCGATCGGACTTGGAGAAGAGAAGAGAAGATGGTTGGCGCGAATCTGAAAGCGGAGA CGATGGCTCTAATGGACAAGAGAACAGCCATGGAGACGGAGATGAACTCTATTGTCGAACGTCTCTGCAATCCCGGTGGT CCTGGTCTCTCCGGCAATCTCATCGACTCGGAGGGATTTCCTCGGGAAGATATTGACATTCCCATGGTGAGAACTGAGCG CCGTCGTCTTGCTGAGTTAAGGAGTGAGCACGGGGAGATAACGGAAAAGATAAATGTGAACATTCAAATTCTTCATTCAG TGAGGCCTACATCTAGAGCTTCATCCACAAAAGATTCAGGTCCAGAAGAAACAAGCTTGTCAGGTGCTGCAGTAAATTCT TTATCTGCCTCCATGCAGACATCTGGCTTCAGTGTTACTTCCGGTCCCATGGATGTGGATGTGGTTACTAGTATTCCCTT TGCAATGGTTGATGAGATAAACGAGTCATCTCCAGCAGCAGAGGGTGGATTACAGCTTGGAGATCAGGTTTTGAAATTTG GCAACGTGGAGGGTGGTGACAACCTGTTGCAAAGGCTTGCCGCTGAAGCTCAGTCTAACCAGGGACAAGCCGTTTCTGTT CAAGTTATGAGGCAAGGTGCAAAAGTCGTCTTATCTGTCACACCAAGGATATGGCAAGGCAGAGGTCTATTGGGGTGCCA TTTCCGTTTGGTATGAGCAGCAGATACTTCCTTGGATTATCATCTGGGGGTCTGGATATGGTGAAAGCTTCTGTGAAAGC TAGTCGGTGATGAAGTCTATTATTGCAAAGTAGAG N...........................................i.................i.............N... .................i...........................i.................................. ....................................................i........................... ..i........i.................N...........N...................................... ...............................................................i................ .....................................................N.......................... ................................................................................ ............i...................................i...N.....N..................... ....i......N.................................................................... ................................................................................ ......N...........................................N............................. ............N............................................N...................... .........N......................... Pos Score Pred ------------------------ 1 0.221 - 45 0.505 Yes 63 0.551 Yes 77 0.455 - 98 0.607 Yes 126 0.722 Yes 213 0.872 Yes 243 0.718 Yes 252 0.555 Yes 270 0.398 - 282 0.291 - 384 0.509 Yes 454 0.367 - 573 0.743 Yes 609 0.685 Yes 613 0.140 - 619 0.208 - 645 0.733 Yes 652 0.135 - 807 0.164 - 851 0.488 - 893 0.116 - 938 0.101 - 970 0.084 -

Explain the output -- Processing locus 893: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTAACAAACAATTAATAATTGGGTCTTGAGAATGATTTTTCTTTTTGTCAAAATATATATATTTGTCATTTTGAATTT AACTAAAATAAATTATTATAAATGAATTTAACAAATTTAATTTCCCTGAATCTGATCTTAAATCCTCCTCAGAAACCCAG AAACGCGAAGATGGGAGACAATGGAGAATCAATAAAATGGCCAAAGATCAAACTCAAGTCGAATCTCAATGTCTCTTATC TTAAGAATCATGATCTCTTCACCGTGGAGAACTGTCTCACTTCTGATGAATCAAAGGCTTTTGTTAAAATAGCAGAGTCT CTTGGTTTTACTCATCAAGGAAGTCGTGGTCCAGCCTATGGTGAAGCTTATAGGGATAACCATCGGATCTCAGTGAACGA TCCTGTCCTTGCTGATACGCTATGGCAATCAGGGCTTTCGAATTTGTTTACTGATATCAAGATTAGGAGGAAGGTTGCTG TTGGCTTGAATCCAAACATTAGATTTTACAGGTATAGTGCTGGTCAACATTTTGGTCGTCACATCGATGAAAGTGCTGAT CTTGAAGATGGAAACCGGACCTATTACACATTGTTGATATATCTAAGCGGGAACAGCACAAAATCAAAATCCAAGAGCAG TTCAAGCAAGACGAACGATTCTTCTTCGGCTGAGCCTTTAGTAGGTGGAGAAACCGTTTTTTATGGTTCAAGGAACAGTA TTGTAGCTGAGGTAGCTCCAGTGGAAGGTATGGCTCTCTTTCACATCCATGGAGACAAGTGCATGTTGCACGAAGGGAGG AATGTCTCCAAAGGCGTCAAGTATGTGTTCCGTTCTGATGTTGTTTTTGCATGAAGAAGACATTCATCTTCTCTGTATTT TGTGTTTCTCACATTAACTACTTTCATACACATTAAGAACCATCGATTAAGCTTGTGTAGATCACTATTTACC .................................N.............................................. .....................i.......................................................... ..........i.........N...............i...............................N........... .........N...................................N.................................. .....................................N.......................................... .....................N.......................................................... ..................................................................N............. .......N........................................................................ ..............................................................N................. .............................N..................N.............N................. ......................................................................... Pos Score Pred ------------------------ 34 0.205 - 102 0.686 Yes 171 0.620 Yes 181 0.245 - 197 0.699 Yes 229 0.153 - 250 0.226 - 286 0.155 - 358 0.119 - 422 0.284 - 547 0.342 - 568 0.442 - 703 0.178 - 750 0.368 - 769 0.202 - 783 0.342 - 802 0.366 - 823 0.269 - 838 0.110 - 851 0.266 -

Explain the output -- Processing locus 894: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGCAAAGTCTCATCCTTTCTCAGCAAAAAACACTAGGAAATGGTTGAAAGGTACAGTGTTCTTCATATGCTTGCTTCTGT AGAGCTGTTTTTTGAAATTAGCAAAACAAGTTCACAATGTTTTATATGCAGGCATGGAGAGTTTTGATTTGCTGCTTAAG ATATATCATGTCGCAGTGCGTTGACGGTATCAAGCATTTATGCACCTCTGTTTTAGGGTGTTTTGATCTTGATTTGTATA AACAATCTGGTGGCCTTGGAGATCCTGAATTGCTTGCAAGAGACACTGTTTTTAGTGTGAGTGAAATAGAAGCTCTTTAT GAGCTTTTTAAGAAAATCAGCAGTGCCGTGATTGATGATGGGCTAATAAACAAGGAAGAGTTTCAGTTGGCTTTGTTTAA GACAAATAAAAAAGAGAGTTTGTTTGCAGATCGGGTTTTCGATTTGTTTGATACAAAGCACAATGGAATACTGGGGTTTG AGGAATTTGCTCGTGCTCTCTCCGTCTTTCACCCAAATGCGCCTATAGATGACAAAATTCACTTTTCATTTCAGCTATAT GATCTTAAACAGCAAGGGTTTATCGAACGGCAAGAGGTGAAACAAATGGTTGTCGCTACGCTTGCTGAATCTGGAATGAA TTTGAAGGATACTGTCATTGAGGATATAATCGACAAGACGTTTGAGGAAGCAGACACAAAACACGATGGAAAGATCGATA AGGAAGAGTGGAGAAGCCTTGTTCTTAGACATCCCAGCCTTCTGAAGAACATGACTCTTCAATATCTCAAGGATATCACG ACTACGTTTCCAAGCTTTGTGTTCCATTCTCAGGTTGAAGATACCTGAAAAAGCAAAAGCAAAAGCAAGCAGCGGCAAGA CATCATTGTATTGTTGAAGGTTGTTCTTTCGTTATGGTTCTTTATTTGTTACATTTCTAAATCTCTG .......................................i..........................N............. ....................................N........N.......N.......................... .......N...............................N........................................ ..............................................................................N. ..................................N..N.......................................... ..............................................................N................. ....................................N...........N.............................N. .............................................i.............................i.... .................................................................N.............. ..................................................i............................. ................................................................................ .................................N................................. Pos Score Pred ------------------------ 40 0.818 Yes 67 0.229 - 117 0.416 - 126 0.301 - 134 0.298 - 168 0.465 - 200 0.143 - 319 0.074 - 355 0.123 - 358 0.190 - 463 0.209 - 517 0.274 - 529 0.147 - 559 0.101 - 606 0.784 Yes 636 0.696 Yes 706 0.216 - 771 0.576 Yes 914 0.058 -

Explain the output -- Processing locus 895: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAAAGCAAATAATTAACCATGACGCGTCTCTGATTTTTCAAACAAAAAAAGCCAAAAATTCGATTTGAGTTTATCATAT CTTCTTCTATAAATAAATTGAAATATTTTTATACACATACGTTGTCAAATGCTAACCACACGTTTGCGTTTAGTAACCGA AAGAACCAAACTGATAAATACTACTTCTCCTATGAGAATAGTTTTTGGTAAAACTTACATAATGGGAATGGTTTTTGGTA AAATCGCTGTAGAGACTCCTAAATACACGGTGACTAAATCCGGTGACGGTTATGAGATCCGTGAATATCCACCAGCGGTT GCGGCGGAGGTTACATACGATGCGTCGGAGTTCAAAGGTGATAAAGACGGAGGCTTTCAGCTTTTGGCTAAGTACATAGG TGTGTTTGGCAAACCGGAGAATGAGAAACCGGAGAAAATTGCTATGACTGCACCGGTGATCACTAAGGAAGGTGAGAAGA TTGCGATGACTGCTCCGGTGATTACTAAGGAGAGTGAGAAGATTGAGATGACTTCTCCGGTTGTAACTAAGGAAGGTGGT GGAGAAGGAAGGAAGAAGTTGGTGACGATGCAGTTTTTGTTGCCGTCGATGTATAAGAAGGCGGAGGAGGCACCACGTCC AACGGATGAGAGGGTTGTGATTAAGGAGGAAGGAGGGAGGAAGTATGGTGTGATTAAGTTTAGTGGTATAGCGTCGGAGA GTGTGGTGAGTGAGAAGGTGAAGAAGCTGAGTAGTCATCTTGAGAAAGATGGGTTTAAGATCACCGGAGATTTCGTTCTT GCTAGGTATAATCCTCCATGGACGTTACCACCGTTTAGGACCAATGAGGTCATGATTCCTGTTGAATGAATTTGATTCAA AGTTTCCTGCTTTTGGTTTCAAAGAACTTGTTTTTATTTTGTTTCACTGTGTTTGTAGTTGTAAGAAGCATAACAATAAT GTATACAA ...................N............................................................ ................................................N............................... ...............................i.............................i.....i............ ...................................................N............................ ...................N............................................................ ....................N......................i.................................... .....i.........................................N................................ ...........................i....................N............................... .....N......................................N................................... ................................................N............................... .................N.........................N.......N.............N.............. ..............................................................................N. ........ Pos Score Pred ------------------------ 20 0.498 - 129 0.391 - 192 0.562 Yes 222 0.815 Yes 228 0.794 Yes 292 0.092 - 340 0.237 - 421 0.114 - 444 0.677 Yes 486 0.668 Yes 528 0.381 - 588 0.622 Yes 609 0.181 - 646 0.185 - 685 0.179 - 769 0.228 - 818 0.271 - 844 0.317 - 852 0.100 - 866 0.476 - 959 0.048 -

Explain the output -- Processing locus 896: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGTTTGATTTCGAGTTCTCTTTTTTTTTGTCACGTTTAGATGAAATTTAATTGGAACAAGCATCTAATTAATGAGTGTT CTTGCTCTAGTTTAAATTTAGTTGAGCACTCTCAATATCTTGGAACACAAAATGACTCTTTTTGGTTTTTGTGATGAATC TTCAGGCCCTGAAGTAGCAAGATTGGCTGACGTGGCTAGGAAAAACCATGTGTACTTGGTAATGGGAGCCATAGAGAAGG AAGGGTATACCCTCTATTGCACAGTTCTTTTCTTTAGTCCACAGGGTCAGTTCTTGGGCAAGCACCGTAAACTCATGCCC ACAAGTTTGGAACGTTGCATTTGGGGCCAAGGGGACGGATCAACCATCCCCGTTTACGACACTCCCATTGGAAAACTCGG TGCTGCTATTTGCTGGGAGAATAGGATGCCCCTCTACAGAACTGCATTGTACGCCAAAGGCATTGAGCTTTATTGTGCAC CTACTGCTGATGGTTCGAAAGAATGGCAATCGTCGATGCTTCACATTGCGATCGAAGGTGGATGTTTCGTCTTGTCGGCT TGCCAATTCTGCCAGCGTAAACATTTCCCTGATCATCCTGACTACTTGTTTACCGATTGGTACGACGACAAAGAACATGA TTCTATTGTCTCCCAAGGTGGAAGTGTCATTATTTCACCTTTGGGACAAGTTCTCGCCGGACCAAACTTTGAATCAGAGG GTCTCGTCACAGCTGATATTGATCTTGGTGATATAGCAAGAGCCAAGTTATACTTCGATTCGGTTGGACATTACTCGAGA CCAGATGTTTTACACTTGACCGTAAATGAGCACCCGAGGAAATCGGTTACATTCGTGACGAAGGTGGAGAAAGCTGAGGA TGACTCAAACAAATAGTAAGAGACTTGAAGTTCGTATCTGCTGGAGTTATGTCAATCGTATGGAGTCAAGTCCAAAATGT TCTGTTGCGTTTTCATTTTATGTTCAAGTTTATTT N.......................................N..............................i........ ...................................................i.....................N...... ...............................................N.............i.................. ..........................................................................N..... ................................................................................ .........................N...................................................... .........N............i............N.........................N.................. ............................................................................N... ................................................................................ ................................................................................ ....N....................N.....................................................N ................................................N..........N................N... ...................N............... Pos Score Pred ------------------------ 1 0.132 - 41 0.236 - 72 0.602 Yes 132 0.722 Yes 154 0.158 - 208 0.407 - 222 0.683 Yes 315 0.389 - 426 0.368 - 490 0.285 - 503 0.706 Yes 516 0.408 - 542 0.118 - 637 0.276 - 805 0.194 - 826 0.314 - 880 0.235 - 929 0.083 - 940 0.042 - 957 0.049 - 980 0.037 -

Explain the output -- Processing locus 897: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTTCATCAACTTGAATTTTGAATTAAACATAATTTGTTCAAGAATGTTTTTTTAAATACTTTTAACCAAGTCAAATAT CAAGTTCATTGTATTACTTAATTTCTTGGATTAGTTCAACACGTAAACTCATCTATAAAATTGAAGTCTATGTCTCATAA GTTTCTTATCTCTTCAATACAATCTTATTCCACAAACATAAACCCTCTTCCTATCTTTTTAATGGCAATGAAGAGTCTCT CATTTCTTGCAGTGCTCTCTCTCTTGGCTTTAACACTTCCATTAGCCATTGCTTCTGATCCAAGCCAACTTCAAGACTTT TGTGTCAGCGCCAACACCTCAGCCAATGGCGTTTTTGTTAATGGAAAGTTCTGCAAGGACCCAAAGCTGGTCACAGCAGA TGACTTCTTTTTCTCAGGGCTTCAAACTGCAAGACCCATTACTAGTCCCGTTGGGTCGACCGTGACAGCCGTCAATGTTA ACAACCTTCTTGGATTAAACACTCTTGGAATCTCCCTTGTCCGTATTGATTATGCGGTTAACGGACAGAACCCACCTCAC ACCCACCCACGTGCCACAGAGATCTTGGTTGTCGAACAAGGAACACTTCTCGTAGGGTTTGTCACATCAAACCCGGATAA TCGCCTATTCTCAAAAGTTCTCAACGAGGGTGATGTATTTGTGTTTCCCGAGGGACTCATCCATTTTCAAGCAAACATTG GCAAGGCACCAGCGGTTGCATTCGCCGCTCTAAGCAGCCAAAACCCTGGTGTCATCACTATTGCCAACACCGTGTTTGGG GCTAACCCAGCCATAAACCCAACTATTCTTGCAAAGGCATTCCAGTTGAACCCAAGGGTTGTCATGGATCTACAGACCAA GTTCAAGAAATAATGATATCGTGATTTGAGTTGCTTTCCCTTTATGTACTTGTGCCTGAATAAGGATCTTGTATTTCCGT TTATCATATCATCATATGATGTCTTTTTTGCT .............................................N.................................. .....................................................................N.......... .............................................................N.....N............ ................................................................................ .........................N..............N......................................N ..........................................................................N..... ...................................................N............................ ................................................................................ ................................N............................................... ................................................................................ ...............................................................N................ ............N..............................N.................................... ...............N..N............. Pos Score Pred ------------------------ 46 0.342 - 150 0.446 - 222 0.290 - 228 0.454 - 346 0.338 - 361 0.439 - 400 0.295 - 475 0.395 - 532 0.262 - 673 0.403 - 864 0.130 - 893 0.288 - 924 0.072 - 976 0.058 - 979 0.052 -

Explain the output -- Processing locus 898: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTTGTATTAATGGGCTTTGATTTTCCGTGAAATTAAAATATAGCGCTATATGTAAACTAAAATCAAATGTTTTTGGAC GACTTCTTGGACTATATATAGCGCATGCATGTTTCTCATGCAACATCGCTTAATCCACAACAGGGAAAAAAAAAAAACAC ACGAACATTTCTGTTTGAGAGAAAAAAAATGAAAGGTCTTCTGCATTTTCTTTTAGCCAAAATCATTTTGTTGGCTTTAG CATCTTCGTTTGTATATTGTTACGAACCATCTCCTCTTCAAGACTACTGTGTTGCCACCAACGAAACTAATGGAGTTTAT GTAAATGGAAAGTTCTGCAAAGATCCAAAATGTGTAACGGCGAATGATTTTTACACTTCCGGCCTAAACGTTCCCGGAAA TACCAGCACCGGTCCGGGCGTCAAAATCACCGTCGTGGATGTCAAAAGAATGCCTGGTCTCAACACTCTTGGGGTCGACA TTGCCCGCATTGACTTCGCCCCGGGCGGTCTATACCCACCTCACACCCACCCACGTGGCAGCGAGATCTTCTTAGTCATG AAGGGCAAGCTTTTCGTTGGATTTGTGTCGTCGAACGAATACAACTACACGCTCTTCACCAAAGTTCTTTATCCGGGAGA TGTATTTGTGTTTCCCAAAGGTTTGATACAATTTCATGCGAATATTGGAAAGACAAATGCGGTTGTGATTGCTGCAACTG GGAGCCAAAACCCCGGTAGGATCATAATAGGTAATGCGGTTTTCGGATCGAAACCTTTGATTGATCCCAAGGTTTTGGCT AAAGCTTTTGCGTTGGACTTCAACAAAGTGAAATATTTTCAGGCAGTGTTCTCTTGATGACATACGTAATTAGCCTCAGT GAATAATTATGAAATATAGTAATGACGAAATAATCTGATGATTATATATTCCGTACAAACTCGATCAATCTATAAT ...........N.......................................N................i........... ........................N...N........N.......................................... ............................N................................................... .....................................................................i........N. ....i........................i.............N.................................... ......................................N..........i.............................. .............................................................................i.. ...............................................................................N ...................................N....................N....................... .................................N.............................................. ........................................................N....................... ........N............N...............N...................................... Pos Score Pred ------------------------ 12 0.384 - 52 0.277 - 69 0.520 Yes 105 0.299 - 109 0.176 - 118 0.128 - 189 0.347 - 310 0.574 Yes 319 0.269 - 325 0.554 Yes 350 0.531 Yes 364 0.328 - 439 0.470 - 450 0.811 Yes 558 0.633 Yes 640 0.130 - 676 0.092 - 697 0.266 - 754 0.195 - 857 0.148 - 889 0.059 - 902 0.187 - 918 0.049 -

Explain the output -- Processing locus 899: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTACTACGCAATTGTCTGTGAAATCACTTTATCAGGAACTTTTGCTTTGGTTCTCTACTTAGATTCTGTATCACTGTGTT CACTGTTGGTTTGAGGATTAGATTCATCCTCTATTAGATTCTGCAAATTGCTTCATGGTTTGGAATTTCACTGGTTTACA GGATATGATGAGCATTGTATCAAGTCATCATCACTGTCAACAATCTGTTATACACGAACGTAGCGGTATAGGAACAATAT CTAAGGTTTCTTGTTCGAGATATTGCGGTCCGTGTTTCTCTGGACGACTGGCTTTCTCTCATAGCCTCTGCTTGAAGACT ACTTATGGGAAAACTGATAGGAGAAAGTTTTCAAGAATTTGCAGTATTGTGGATGATGAGGGGAATCTAGATAATTCGGA TGACGAGGAAAAGGAATCACTCGATGATAAAACTAAAAGGCAAGCGAATGATATGAATCGGGCTAATCTTGAAAGAATGG TTGGGTCAGATGATTCTGCATTTAACGGCTTAGATCTTGCAACCCTCATCAGACAGAAGTATGGAAAATCTTATGATGTT CAACTCATCAAGAAGGAGTTCATGGGGAAGAATCTTCTTGCAATGAATGTCATGTGGAAGTACAGAGAACAGAGGTCTTT TCCTCTAACCGAAGAAGAATATATACTTAGACTTGACGATGTAGCCAACATGTTGAAATGCTGGGGAGCAGTCTCACATA TCCGTAGCAGCCTAGCAAAGTCGAAGGAGCGACCTCGGATAGGGAAGGCTGTCAGCATTTTCATAGACATGGATTCGACA GGGGGAAGAGCAAATGAATGGATTTACAAGTAAAAATCGACCGGTTTGCAGCTGCTAAACCAAGAAATCAAAAGGTTTTG TCTCTACGTTTATCGTCATCAATTCATTTTGAAGGCTCTTCAGTGATGGTTG ................................................................................ ......................................................i......................... ....N..N........................................................................ ................................................................................ ....N...............................................N..N.......................N .......................N.......................N....N.......................i... .........N..................................................N...........N..N.... .....................N....................N...N....N............................ ......................................N..........i.......i...................... ....................................................................N........... .............N...i.............................................................. .............................................N...... Pos Score Pred ------------------------ 135 0.630 Yes 165 0.331 - 168 0.389 - 325 0.309 - 373 0.228 - 376 0.180 - 400 0.116 - 424 0.072 - 448 0.128 - 453 0.454 - 477 0.681 Yes 490 0.309 - 541 0.164 - 553 0.131 - 556 0.440 - 582 0.437 - 603 0.165 - 607 0.317 - 612 0.445 - 679 0.436 - 690 0.529 Yes 698 0.743 Yes 789 0.369 - 814 0.481 - 818 0.586 Yes 926 0.067 -

Explain the output -- Processing locus 900: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATATTACAAATCAATAAACTGCAAGCTCTGGTCATTGAATTGTGTAAAATCCAATTTTAATAAAAGATTGATTATCGAT AGACTGGTTAAACGTTTTTGAACTGCCACTTTGAAACATGTGAGTTACAAAATGTATATGAATAACTTGGACCACGTCAA ATATGACACCGGCCTTTTTCCTATAAATACTGAGCTCGAATGCTTCCTCATTCACAAACAACACACTACATATCTAGAAA GCATTAGAACAAAAAAAAACCTTTGTTTGAAAATGAAGGTGTCCATGTCTCTCATCCTGATAACCTTATCAGCTTTGGTC ACCATTGCCAAAGCATATGATCCAAGTCCACTTCAAGACTTCTGTGTGGCCATTGATGACCCTAAAAATGGTGTTTTTGT GAATGGTAAGTTTTGCAAGGATCCAAAGCAAGCAAAGGCAGAAGATTTCTTTTCCTCAGGCCTCAACCAAGCAGGAATCA CTAATAACAAAGTCCAATCCAACGTGACAACAGTCAATGTCGATCAGATTCCAGGGTTAAACACTTTGGGAATATCCTTG GTCCGCATAGACTATGCGCCATATGGTCAAAACCCGCCTCACACACACCCTCGTGCCACTGAGATCCTTGTTCTTGTTGA AGGAACATTATATGTTGGTTTTGTCTCTTCGAATCAAGACAACAACCGTTTATTCGCCAAAGTGCTGAACCCGGGAGACG TGTTCGTGTTCCCCATAGGAATGATCCATTTTCAAGTGAATATCGGGAAGACCCCCGCAGTGGCCTTTGCTGGACTAAGT AGTCAAAATGCTGGTGTCATCACGATCGCAGATACAGTGTTTGGATCAACGCCTCCGATTAATCCAGATATTTTGGCTCA GGCGTTTCAGTTAGATGTCAATGTTGTCAAAGACCTTGAGGCCAAGTTTAAAAACTAAATCCCCAATATTGAGTGTTTAT CGTTGTAACACTTAAGTAATCTGTAACACGGAGTAAAGGTTTATGCTTAATAAAATAAAAAGGTGTTATCACCTAAT ................................................................................ .....................................i.............N.....N...................... ..N....................................N........................................ ................................i...........i................................... ................N......................................N...........i............ ..i............................................................................. ....................................i........................................... .............N........N......................................................... ...........N.................................................................... ....................N........................................................... .......N........................................................................ ..............N.....N........................................................... ..........................................N.................................. Pos Score Pred ------------------------ 118 0.723 Yes 132 0.474 - 138 0.292 - 163 0.167 - 200 0.293 - 273 0.679 Yes 285 0.657 Yes 337 0.311 - 376 0.439 - 388 0.545 Yes 403 0.591 Yes 517 0.506 Yes 574 0.142 - 583 0.372 - 652 0.267 - 741 0.277 - 808 0.162 - 895 0.189 - 901 0.134 - 1003 0.032 -

Explain the output -- Processing locus 901: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAACAATGAATCCTTAAAAAAACTGTTTTTTTTTGCTCGAATTGTATAGTTTTGTGATGTGTATTAGCTTATTCAAGTT TAGTTATTCATTTGGTTTTAGAAATGTTATATGTTTCTCTTACAGGTGATTCTACTGTGTAGGAGGAACAAGAGTTTTGC ACCAAGAAGAGAGAGGAAGGATTCTGGTTTACGGGAGCTAGTGAAAATCTTCTGTGAAATTGGCTTTCCTCTCTCATTCT CTGTGAAATTTGAAAGTATTGATTTCGAAGATATGGGTGCTTTCTGTTGTTGCTTTCAAGTTGATCTCTTTGAGAGTTAT GTGAATCCAAACACTTCCATAACTAGGAACTGTCCTTGCCTTAATTGCTTCCTCCAGAGTTTCATGGATTTGTATGCTTC GTTGTTTAGTAGAGGGGGAATGCATCCAATACCTTCAACTGTAGAGACAGCTACAGTGATGAATTCAACAACTGCACTTG ATGACTCTCTGTCTAGTGTATATCATTCTCCTCCAACACCATTGCCTTACGATGCTGATCCTAGGTACTTTCGTTTTGTT AAGGGCTCTAGTCATTCAGGTGAAGAATCAGAACCGTTAAGAGGTGATACTGAAATGAGTTCTGAAGCTTTGGGTGATGG TGGAGCCAAGTGGAGTAAGTCTGATTCTGAAGATGGTTCGAAAGAAGTGTATACGAAAGGTTCATCAACCTTTACAAAGT CGAAAACAATGCCGGGGATAGAAGTTTATTATGCAGATTCGGATGATGAAGATATTTGTCCAACCTGCCTTGATGATTAC ACTTTGGAGAATCCGAAGATAATCACCAAGTGTTCTCACCATTTCCACCTTAGCTGTATTTATGAATGGATGGAGAGAAG TGAAACTTGCCCCGTCTGTGGAAAGGTGATGGCATTTGATGAAAATGAAACTTCTTAGCATTGTAATAAAGACTTGCTCA AAGAACATAATGGCGTCTGAGTCCTGCTCAAAAGAATAAACAGAGAGAAATCACTTGTACATAAAACTCCTGTGAAT ......N..................................................N...................... .......................i......N................................................. ................................................................................ ................................N.............................................N. ...............................................................i.........N...... ...................i......................................N..................... N..................................................N............................ ......................................................i.....................N... ................................N............................................... ........i.....................N...........N..N..........................N....... .............................................................N...i...i.......... ............................i.........N.....N................................... .........N................................................................... Pos Score Pred ------------------------ 7 0.257 - 58 0.270 - 104 0.699 Yes 111 0.230 - 273 0.499 - 319 0.191 - 384 0.742 Yes 394 0.106 - 420 0.576 Yes 459 0.398 - 481 0.215 - 532 0.416 - 615 0.659 Yes 637 0.348 - 673 0.300 - 729 0.815 Yes 751 0.211 - 763 0.152 - 766 0.179 - 793 0.177 - 862 0.269 - 866 0.542 Yes 870 0.582 Yes 909 0.758 Yes 919 0.223 - 925 0.252 - 970 0.055 -

Explain the output -- Processing locus 902: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTCTCTCCTACAATTAAGCTCTTTGACGTACGTAATCTCCGGAACCAACTCCTATATCCACCATTTACTCCACGTTG TCTCCAATTATTGGACGTTGAAACTTGACACAACGTAAACGTATCTATGTGGTTGATTGTATGTACATATGTACAAACGT ACACCTTTCTCCTCTTTCACTTCATCACTTGGCTTGTGAATTCATTAATTCCTGCGAAATGGAACCTCGAATCAATGACC TTCAGGTGGAATCTCGGGTTCATGAGTTGCTCGATTTTCCGGTTCATACAAACCAGATATCCTCTGCAATCTACGAGATC CTATTGCAGTGTCCCAACGACCACATCGAAAACCCTAAGAAGAAACCGTACAATTGTCCACACTCAGGAGCCAAGTGTGA TGTCACAGGAGATATTCAAAGGCTACTACTGCATCTAAGGAATGATCACAATGTTGAAATGAGTGATGGGCGTAGTTTCA GTCACAGATATGTTCATCATGATCCTAAACATCTTCATCATGCTACTTGGATGTTAACACTTCTCGATTGTTGTGGTCGA AAATTCTGCTTGTACTTCGAAGCGTTTCACCTGAGGAAAACACCAATGTACATGGCCTTCATGCAGTTTATGGGAGATGA AGAAGAGGCAATGAGCTTCAGCTATAGTTTGCAGGTCGGAGGGAATGGCAGAAAGCTGACATGGCAAGGCGTGCCGAGGA GCATTCGTGACAGTCACAAGACGGTTCGTGATAGTCAAGATGGCTTAATCATCACTCGCAAATTGGCTTTGTTCTTCTCT ACCGACAACAACACCACCGACAAGGAGCTGAAGTTGAAGGTTTCAGGTCGTGTATGGAGAGAACAGCCTGTTTCGATATG AATTCGATAATTAGGTTACAGAATTGGACAACTATAGAATATTTGGTGACAAAAATATCTTTTCTTGATTTAAAAATTAA ATATAGCAATTTCGAGGGAA ................................................................................ ..............................................N.............N.......N........... ..........................................................i...............N..... .....................N.......................................................... ...............................................................................N .........................................N........N.......i......i.............. .........i........N....................N..........N............................. .............................................i.....i........N........N......i... ..........i.................................N...............N................... .......................................N........................................ .....................................................N.......................N.. ................................................................................ .................... Pos Score Pred ------------------------ 127 0.310 - 141 0.272 - 149 0.406 - 219 0.769 Yes 235 0.280 - 262 0.224 - 400 0.376 - 442 0.140 - 451 0.295 - 459 0.506 Yes 466 0.501 Yes 490 0.523 Yes 499 0.211 - 520 0.228 - 531 0.171 - 606 0.624 Yes 612 0.616 Yes 621 0.285 - 630 0.404 - 637 0.663 Yes 651 0.591 Yes 685 0.471 - 701 0.273 - 760 0.358 - 854 0.283 - 878 0.233 -

Explain the output -- Processing locus 903: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGCCTATACAGGGATCGAACCTGTGACCTTCGCGTTATTAGCACGACGCTCTAACCAACTGAGCTAATAGGCCACTTGT TATATTGTTTCCATTGTATGAATATATTCAAAACCTATGAATTTGTCACTATCTCTGCTTCAACTAACACAAACCTTTGT ACTCTCCGCTCCTTCACCTGCAAAAACAACATCTCTCACATTCTCTCTAAACTCTCTTCACTGTTACTCGACAATGGCTA CTACTTCTAACTCGGTACTTTTCCTAAGTTCTGACTCACTGATTCATCATCATCATCATCAACCTCTCCATCTATCTTCC TCCAGATCTCACTCTGTTTCTCTCCCACCCAACAAGCGATCCAATTCTCTCACGCTTCGTTGTTCAACAAATGGCGATAG CACCTCAACCGAAAAGGAAACACCAATCGAACTCAAATTCCCAGCATTTCCAACGGTAATGGACATTAACCAAATCAGAG AGATATTGCCTCACAGGTTTCCGTTTCTGCTGGTAGATAGAGTGATAGAGTATACGCCTGGTGTTTCAGCTGTAGCTATT AAGAATGTCACCATCAATGACAATTTCTTCCCTGGCCATTTCCCTGAACGACCAATCATGCCTGGTGTTCTCATGATTGA GGCAATGGCGCAAGTCGGAGGTATAGTAATGCTGCAGCCGGAAGTAGGAGGCTCTCAAGATAACTTCTTCTTTGCTGGTA TTGATAAAGTGAGATTTCGCAAACCAGTGATTGCAGGAGACACGCTAGTCATGAGGATGACTCTTCTCAAGTTCCAGAAG CGGTTCGGGCTCGCTAAAATGGAAGGTAAAGCTTATGTGGGTGGTGCTCTGGTGTGTGAAGGCGAGTTCATGATGGTTTC TGCTGGTAGTTCTTGATCTCACCCATTAATGGTCTCACTTCCCACATGTGGACTCTTCTTATGCTTCTTCTGTTATTCGA AAATCTAGACAAGACAGCTTTATGTTTTCGACATT ................................................................................ .................N..................N........................................... .........................................................................i...... ................................................................................ ......................................................................N......... ..........................................................i..................... ................................................................................ ....i...........N........................................i..............i....... ....i.......................i................................................... ..................................................i.....i....................... ..................i...............N..................................N..N....... ............................N................N..............N................... .....................N............. Pos Score Pred ------------------------ 98 0.474 - 117 0.382 - 234 0.825 Yes 391 0.351 - 459 0.653 Yes 565 0.556 Yes 577 0.258 - 618 0.887 Yes 633 0.714 Yes 645 0.733 Yes 669 0.659 Yes 771 0.574 Yes 777 0.590 Yes 819 0.562 Yes 835 0.243 - 870 0.149 - 873 0.328 - 909 0.083 - 926 0.068 - 941 0.064 - 982 0.049 -

Explain the output -- Processing locus 904: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGATGATTGGCGCATTCCCAGATCCAATCGATTCATTTATTTCGTCCCCTTGTTATGTACATATACACTTTGCTTTCCGT GATATTGTCTGGATATATTAATCAATTTTTCCTCATGAAGAACTCAAATATTGTGTTATGTTCATAACTCAAATCATTGA ACAATTCAATTCATTTGTGTCCCATTTGCACCTCTTGAAGCAATTAGTAAGGAACGAGCTTTTAAACATGGGAGACTCTT CGAGTTCAACCTCTTGGAGCAGTAAAAAGGATACGGAAGATGATCGGATGATTGCTTTTATGTTATCTGAAGAGTACTCT AAACTAGACGGTGCGGTCGGCAGACGCCTCTCAAATCTTGCTCCCGTCCCTCATGTTCCACGAATAAATTGTTATATTCC CAACTTAAATGATGCCACCTTGGATCACCAACGCCTTCTTCAAAGGTTAAATGTTTACGGTTTGTGTGAGTTGAAGGTCT CTGGTGATGGAAACTGCCAGTTCCGAGCCCTTTCTGACCAGTTATACCGATCTCCAGAGTACCACAAGCAAGTGAGGAGA GAAGTTGTTAAACAGCTCAAAGAGTGTCGTTCTATGTATGAAAGTTATGTTCCGATGAAATACAAACGATATTACAAGAA GATGGGAAAATTTGGAGAATGGGGAGACCATATTACCCTACAAGCTGCGGCAGATAGGTTTGCAGCAAAGATATGCTTGC TGACATCCTTTAGAGACACTTGTTTCATTGAAATTATACCTCAATACCAAGCACCTAAGGGTGTGTTATGGTTAAGTTTC TGGTCAGAGGTGCATTATAACTCTTTATACGATATTCAAGCTGCTCCAGTTCAGCATAAGCCAAAGAGAAAACATTGGTT GTTCTAGAAGGCACGCCACCATACATGTATTATATTAAGACCCTTCAACTATTTTTCAGTCAGTTTGTGATAAGTCTACC GTCTCATACGCGAAAACTCTTCCTTT ..N...................................................N......................... ..................................i......................i...................... ...................................................................i............ .......................................N.......i...........N.................... ....................................................N........................... ........N..i......................................i............................. ......i......................................................................... .................................N...N........N.......N......................... ...................................................................N............ ................................................................................ ........................N....................................................... .......................... Pos Score Pred ------------------------ 3 0.300 - 55 0.182 - 115 0.613 Yes 138 0.533 Yes 228 0.657 Yes 280 0.371 - 288 0.539 Yes 300 0.276 - 373 0.192 - 409 0.453 - 412 0.586 Yes 451 0.776 Yes 487 0.661 Yes 594 0.446 - 598 0.341 - 607 0.385 - 615 0.431 - 642 0.367 - 659 0.348 - 713 0.398 - 788 0.490 - 905 0.076 -

Explain the output -- Processing locus 905: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACAAATATATATATGTAAAGACATATTTTCACCAGCGGTCCAAAAAAAAGGATATATTCACATATTAAAAGAAAATATT GGGGCAAAGTGGATAAGATAACATCCAATGAGAATAAGACATATCAAAATTCATATCCCTGAGAGCCACAACAATCTTAA GAAACAGATCCTCTGTCTTGCCACATCAGCAAACGCAATCTCAAGTTTCGTAGATTGCTTATTTCTTCATCACACACTTC AAACTCGATTTTATTTTAATTTTCATAAGAGAACAACCAAGACAAAAAGAACAATGGCAGCGAGTGCATCTCCAATGGCG AGCCAGCTAAGGAGCAGCTTCTCCTCCGCTTCTCTTTCTCAGCGTCTCGCCGTCCCGAAGGGCATCTCCGGTGCTCCTTT CGGTGTTTCTCCGACCAAAAGAGTCTCTTCCTTCACTGTCCGAGCTGTTAAATCCGACAAGACAACATTCCAAGTAGTTC AACCAATCAACGGTGATCCATTCATCGGAAGTTTGGAAACTCCGGTGACATCAAGCCCATTGATCGCGTGGTACCTTTCC AACCTCCCTGGATACCGTACCGCCGTCAACCCTCTCCTCCGCGGTGTGGAAGTGGGCCTGGCCCATGGTTTCTTCTTAGT GGGCCCATTCGTCAAAGCTGGCCCATTAAGGAACACTGCTTACGCCGGGTCAGCCGGGTCTTTAGCCGCGGCTGGGCTGG TAGTCATCCTCAGCATGTGCCTCACCATCTACGGTATCTCTTCTTTCAAGGAAGGAGAGCCATCGATCGCACCGAGTTTG ACTTTGACTGGGCGGAAGAAGCAGCCTGACCAGCTTCAAACGGCTGATGGATGGGCTAAGTTCACTGGAGGATTCTTCTT TGGAGGGATCTCTGGGGTGACTTGGGCTTACTTCCTTCTCTACGTTCTTGACCTTCCTTACTTCGTCAAGTAAATGTAGA TCAAAATATATCGAGTGTACTTTCAACTCTCTTGCATCATTGTTTTTTACTCTTTGTCTTGATCAAGAATCTTGAAACTC AGGATTGTTAAT .............i.................................................................. ...........................N.................................................... ................................................................................ .....................................................i....................i..... ................................................................................ ................................................................................ ................................................................................ ................................................................i............... ................................................................................ ..............N................................................................. ..............................................N...N............................. .........................................................................N...... ................................................................................ ............ Pos Score Pred ------------------------ 14 0.682 Yes 108 0.352 - 294 0.918 Yes 315 0.804 Yes 625 0.571 Yes 735 0.289 - 847 0.293 - 851 0.219 - 954 0.222 -

Explain the output -- Processing locus 906: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTTTCGTTTGATGTCAACTATTTTTTTGATTGGACCAAGTATTCTGTTAAACAGATAAACTAATAACTAAAACATATA TATACATAAACACAGGGATGAATCATGTTATTTTCGTCGTGCAAAATAATTTTCCAATATACCCTCAAGGAAATGTCATC CCACGGCCGAGAAGTAGAGGTGACCATCTCATCGGCAAAGGATATCAAGAACGTGAACTGGCGCAACGGCCCAAACAAGC CATACGCCGTTGTATGGATCGATCCGAAGTTCAAATCCTCCACAAGAGTTGACGAAGACGGCAACACTTGTACAACGTGG AACGAGACATTTGTCATCGCTTTACCTCCGGCCAACGACGATGACGACAAGGTATACATCAACATCGTACACGCAGGCCG TGAAGAGAACACAAAGCCCCTCATCGGCTCCGCCCATCTCAGCCTCCGTGATGTCATTGATGACGTCGGCTTCGGCGTCC CCTTCATGAAAACCCTCAAGCTCAAACGCCCCTCTGGCCGACCTCAGGGCAAACTCGACGTCACTGTCACCGTTCGAGAG ACTCCAGGATCAAATTACGCGCTTCCTTATGGCGATCCATACGCGCCGGAAAAGGGGAGCAAGTTCGGTGGGATGGGGAC TGGACTGGCGGTCGGGGCGGTGGCCGGAGTTTTGGGTGGGGTGGCATTAGCGGAGGGACTGGAGGCTGTGGAAGGCGATG AGGATGATGATGATGACGATGATGCTGCTGCTGCTGCTGCTGCTGATGATGATGATGATGATGATGATGATGATGATGAT GATGACGAGTGAGACTTTGAGTTTACAGCTATATCATCTTTCATTGGTGAAGTCCTGAATTCTGTGTTGCAACATAACAA GCCTAATAACTTCGATTGAAAAACAAGTAAC ............N................................................................... .................i......N...............................................i....... ................................................................................ .............i.................................................................. ........................................N....................................... ..................................................i........N.................... .....i.......................................................................... ............................N...........................................i....... .............................................................................N.. ...N..N..N..N.....N..N.......................N..N..N..N..N..N..N..N..N..N..N..N. ............................... Pos Score Pred ------------------------ 13 0.171 - 98 0.514 Yes 105 0.498 - 153 0.508 Yes 254 0.558 Yes 361 0.230 - 451 0.716 Yes 460 0.389 - 486 0.677 Yes 589 0.456 - 633 0.812 Yes 718 0.183 - 724 0.076 - 727 0.078 - 730 0.108 - 733 0.104 - 739 0.082 - 742 0.061 - 766 0.046 - 769 0.050 - 772 0.050 - 775 0.052 - 778 0.069 - 781 0.064 - 784 0.069 - 787 0.065 - 790 0.156 - 793 0.066 - 796 0.073 - 799 0.054 - 802 0.127 -

Explain the output -- Processing locus 907: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTAGTATGCAATTATTTTATTTTGATTAATAAAACAAGCATGAAGCGCGTCGTTCGTTGGAACTATTCGTTAATCATTAA GGTGCAAAGAAGACCAACTAGCACCGTAGAATGAGTCGTCGTTGGTTTACGCAATCACAAGAAAGACAAGTCAATGACAA TGAATTGTCAACGCCTTCGTGTTTACACGTCTCTTACCTCTCTCTCTATCTTTCAATAATCTCTCTTTGTTTCAGACTCC TTGAAGCAATTCTTCATTCTGGCTGCTTGACATTCAGAAATATGGCAAATCGTCCACACGTGCCCAAATTCGGAGACTGG ACCGAAGATGCTCCATTCACGGTCGTGTTCGAAAAAGCAAGCAAGAGCAAGAAAAATATGAACGTGGCCAACCCGAATGA ATATCCAGATATGAATCCAAACGCTGCACAAAATCGAAATATGTCTAGGCCTGACCAACAACCACCAAACCATAATGTTA GACCAAGACATGAAAGATTCAATAGCAGAGACGAAACCGAATTCAGACCGTCTCCTGCACACAATGAAAGAAACAACAGA GTTAGATCAGTTCCTCCCACACCAGAAACATACAACCATCAAACATATGGTGGAGGTGGCAGATCAATGGGAAATCCAAC AGAAATAAATAGACGGCAATCACGTGATCATGTCCCGGCGCGGCCAATACGCAATCTTAGAGGGCAAAGTAGTGAAAGGG TGGCGACTATTCCTCCATTTCCTGGAACGGGTTCTAACATGGAAAATCAGAGTTACACACTTATCTTTGACAAAGTGAAA GAAGATAGGAACCAAGCGAGATCTTACAATGGAACCGATCATAGCACCCCGACTCGACCCATCATCGACCAACATCATCA ACCATTACCTTCTAGTCCCAAGGGTTGCTGTTTTCCTCCATGGAGCCGAAAGGGAAGCTAATGAAGATGATCATTTCCCT TTTTTACATAATCCTCTTTTTTTTTATTTGCTTCTGCTTGATTTTTTTTAATAATATCTTTGTTATTCTTATGGAGATGT .....N.................................i........................................ ..............................N..........................................N.....N ................................................................................ .........................................i...................................... .......N.................................................i..................N... ..........N.............................i.................................i..... .........N.....................................................i................ ..............................................i...................i............. .............................N.................................................. ......................................i......................................... ............................i................................................... .......................................N....................N.....N............. ......................................................................N.....N... Pos Score Pred ------------------------ 6 0.409 - 40 0.674 Yes 111 0.440 - 154 0.276 - 160 0.396 - 282 0.878 Yes 328 0.168 - 378 0.723 Yes 397 0.463 - 411 0.496 - 441 0.669 Yes 475 0.676 Yes 490 0.336 - 544 0.525 Yes 607 0.857 Yes 627 0.660 Yes 670 0.319 - 759 0.854 Yes 829 0.662 Yes 920 0.144 - 941 0.172 - 947 0.104 - 1031 0.050 - 1037 0.042 -

Explain the output -- Processing locus 908: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGGCGAGTGGGATAGTGTATCACGTGACATTCGCAGACACAACTCGCTTCATAACTGAAAAAATTAATAATAAAAAATG GATATGTTTGTCTTTCTCTAATGTTTCATGAACATCTGTAACGTCAAACCCACTTTAAAAGCTCTGCTTTTTGTGTTTGT TTTTGCTTACTCTCAAAGAAAGTGGTTCAATCTCTCGATTTCCATGGCGAGGAATCATCAGTTCCGTGTGGATTTGGAAG AAATGGGGTTTCGTTCTCGTTCTCATCTGTTCCTCAACGAGTTCGAAACGTCTGCCGGAAGATTCAACTTGCTCAGCCGT AACCACCGTGATCATCTGTACGTTTCCGACGTTGTGGATACACTCTCTGAACAGTTCTCTTCGATGTCTCTTCTCCATCC TAAACCCAGACTGGTTCCTACTTTGCCTTCGAGTCTCCAGTCTTCTTTAAGGCCTAATGGTGTTAAAGCTTATCAGAGAT TGTACCGAGAAAGGCTCAAGCTTGAACCTTTTTATGAACTTCATCTTTCAAGGATCCGCAAAGAGTCACAGCAAGATAGA GCTAAGGTTTTACAGAGACAAGAGGCACGGCTACAGAAGAGAGGTTTGGTTCACCGAAACGGTAACGTTTTGAAGGAATC TGTAGGAACCGGCGTTTTTCACCCTCTTCAGCGTCCAACTTTCAAGACTTTTGACCCTGTGAAGAAGCATAATCAAAGGA ATCGGAATCAAAGTGAGAAGGAGTTTATGACCAAAGAAAAAAGAGGAGTGATGACTTCTACAACGCAGGAGCAGCAACAG AAAGAGGAGTGTCATTATCATTTGCCTTCAGACATGGATTTCTCCAAGGACTGGACTTTCTGATGGAACAAGTTCAAGTA GTTACTAATTATTAGATCTTTTGGGTATTAAGTATTAACTAGGAGGTAAATAATAATTAGGGACAAAGTTTGAAGATGAC CA .............................................................................i.. ...N................N......N.................................................... ...........................................i.................................... ..i............................................................................. ...............................................................i................ ........................................................i....................... .................................N.............................................. ................................................................................ ................................................................................ ..........................N.......................N............................. .................................N............................N................. ...........................................................................N.... .. Pos Score Pred ------------------------ 78 0.724 Yes 84 0.333 - 101 0.157 - 108 0.292 - 204 0.559 Yes 243 0.633 Yes 384 0.501 Yes 457 0.712 Yes 514 0.298 - 747 0.484 - 771 0.441 - 834 0.151 - 863 0.452 - 956 0.069 -

Explain the output -- Processing locus 909: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTTCGTGCTGTGAAAAATGGGAAAAGTTTTATGATTTTACAAGTTAGAACAGCAGACTGCTCACGAGGTAATACCTTTA TTCTCCTCTTTCTTTAGGTAGGTCTTAGCTCGACGTCAAGAACTCACTAAAATCATGCTGCTCACTTCTTGTTTCGCTGT ATACGTACACTACTCCTCCACCATTACCTCCTCAGCTAGTACATTATCTCAATCTATAACAACTTTATATTACAATATTC CTCACATATACAAGCTTTGGTTATTCGTTTGCTCTTGTTTTTATCAGGTAAGCATCATTTGTAGACTACATTTTCAGAGA GAAATGGGTATATTCCCTGGCTTGATCAATCAGAACACACAACAGCCTCCAGAGGTCGAGTCCAAGAGATCTAGGAATGT TAAAACTAAGTCAGTGTCTGAGACTTATCCAGAGAACAGAGAAGAATTAAAGAAGCAATTAAAACTTTGGAGAGCTTCAG ATAAGAAATCTCCATGGTATGATTATCCTCCTAAAGTTAAGGAACACGTATCAAGTAAAGTTTTTAGCGATCTTGGACCG ACAGAGAAGCACGTGAGGGTGGAAAAAGCTGCTCCCTGGAGGTTCCTTTGGTGGTCTGGATCTATCCCGGTACATCTAAC TTTCAATGAGAGCCGGAAAGATTTCAGCACACTATATATGATTCCTAAAAAGAACGTGATGTTCATGAAAACGTTCTATG GTAAGTGGCAAATAGAGCCATGGTACGTAGATAACATGCGCTTTTGCAAGCCGAGGCTCCCAAAGAATCGAGAAGAATAT AGACAATGTACCGGTGGAAAAGGATTGATTGGCTCGAGAGTGACATTGGACCAGTATTTTCAGCCTTCTTCTTATCTTAA CCTGCCACCACTTTCTTGGTACATCCGTAGGGCCACCGTCAAGACCACCAAGGCTCTAATCGAAGATCTTCAAATTCAGG CCGCCGTTATCAGAAGCGTTTGAAACTCTTTCCAATCCGATATTTGCTAGTTATACCATTTTTATACAAACATTAACCTC AAATATTGGAATACTGTAAAAACAATTTCACGTTCTGTCAAG .................i.............N................................................ ......................................................N......................... ................................................................................ ................................................................................ ...i........................................................................N... ................................................................................ .............N....N............................................................. ................................................................................ .....N...............................N....................N.....N............i.. ...................N...............N............................................ .....i.......................................................................... ................................................................................ ................................................................................ .......................................... Pos Score Pred ------------------------ 18 0.505 Yes 32 0.303 - 135 0.469 - 324 0.696 Yes 397 0.421 - 494 0.268 - 499 0.157 - 646 0.222 - 678 0.368 - 699 0.297 - 705 0.252 - 718 0.583 Yes 740 0.446 - 756 0.288 - 806 0.667 Yes

Explain the output -- Processing locus 910: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAGTTTTTCATTTGCGGGATTGGTAGTCGCGATCGTATCAAGAGAAAATGCATAAGAGAAATGAATAGAGACATATTGA AGGGTGTAGTGATCACGTTAAGACTTACCTGTCCACCAATCTGTCACTCTTTATTTTACACAACTTCTTTATTATTATTC TCTCTCACTTTTTATATATAATTCAATTCGCTCTCCTTCTCTCTCTATCTCTGCCCTTTCTTTCTCTCTCACACACAAAC ACGATCCAATGACGACGAGCAACAACGTGAACGGATGTTTCGGAGACACGAAGCTGACCAAAGTGTTCGTAGGAGGATTG GCTTGGGATACTCACAAAGAAGCAATGTATGATCATTTCATCAAGTATGGGGACATCTTGGAGGCAGTCATTATCTCCGA CAAACTCACTCGCCGTTCCAAAGGATATGGCTTTGTGACTTTCAAAGATGCGAAAGCTGCCACGAGAGCTTGCGAAGACT CTACTCCGATCATCAATGGCCGCCGTGCCAACTGCAATCTCGCCTCCCTCGGTGGCCGTCTTCGACCAAAAAATGGGAAC AGGGCCACGCCACCACATGTTGGAAATCATTCTCAGTGGTACTACCCGTCCGGATTCACAAACCAGCAACATCAACAACA AAATCATCAAGCCGTTCCTTTCTATGGGTACCCTTCCGCCTACGTTGCCCCGAACATGACCTTCAATCAAAAAGTTGGAT ATGTCGGAGGAACCTATATGAACGGATACTATGCACAAATGCAACCACAACCGCAATCGCAGCCACAACCGCAATATTAC CACCATCACATGTATGGTGGAGGACGAGTTATGGTTGGGGCAGCAAGTCCAATGGTGCCTTTCTACACTGTCTATCCTTA TCATCAATCTCAAGCCATCGGGTTTCCACAACCTTCATTTTCGAAACCTCTTTCTTTTTCCACTCCTCCTATATCAGGCA CAGTTGGAGGAGAGAGCATACAGAAGAAGGCCATACATTGATAGGCTAGAAGCTGTTAATCAAACGATTGACTCAGCCCA AAATTATTCATTTTATCCATCTCACTAAGCTTTATTTTTTTCTAGAGACTTGTATTTGTA ................................................N............N.................. ................................................................................ ................................................................................ ........i.........................N............................................. ........................i...N.................N................................. ..........................i....................i................................ ...............N........................................................i....... ................i............................................................... .......................i...............................i........................ i................i............N.......i......................................... .........i...N................i....................i............................ ................................................................................ ................................................................................ ............................................................ Pos Score Pred ------------------------ 49 0.202 - 62 0.149 - 249 0.823 Yes 275 0.365 - 345 0.783 Yes 349 0.440 - 367 0.284 - 427 0.710 Yes 448 0.741 Yes 496 0.415 - 553 0.881 Yes 577 0.662 Yes 664 0.786 Yes 696 0.567 Yes 721 0.781 Yes 738 0.608 Yes 751 0.346 - 759 0.739 Yes 810 0.538 Yes 814 0.432 - 831 0.786 Yes 852 0.500 -

Explain the output -- Processing locus 911: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACTTATCATTAAATCTTATTATCTTTTACGGGTGTTTTCATTAAAGTAAAATAAAAATAAATAAGAACAGTACAAAACT ATAGCTTACGTATCTGAATAGTTCCTCCAAATGACGATCCTACCCTTCACAATCTCTCTTTGTTTACTCAATTCCATGAA CCGCTATAAATACGCTCTAAACGTTACTCGTCACTTGGCTTGCACAGAACAAACCAACCTTCTAGATTCTTCTCCTTCCT TCTTCAACTCTCTCTCACTTCTCCAGATATGGTTACATCTCCGACGAACTCAACAATCAAATTTCTCTGTAGCTATGGCG GCAAAATCCTACCTCGTTATCCCGACGGTAAACTCCGTTACAACGGTGGTCACACTCGCGTCCTCGCCGTTCCTCGCTCC GTCTCATTTTCCGGTTAGTTTCACGCTCTCGATCTCATTGTCAATTTTGAATTTCACAAAAAAAAAAACGAAATCGGTTT TGTGGAAAGAGAAATTAAGGCCACACGTGTGGTGCGTTTTAACAGAGTTAGCGTCGAAGATGGCGGAGATGTGCGGCGGC GTCGGCGGATCTATAACCGTAACGATCCGATGTCAGCTTCCGACGGAAGATCTCGACGCGCTCGTTTCAATTACTTCCGA TGAAGATCTAGTGAATCTAATCGAAGAGTACGATCTCGTTTCCTCTTCATCTCCGATGAAAATCAGAGTCTTCTTAAATC CACCAAAATCCGCCGCCGGATCTAAAAAATCTCCTCCTCCGTTAGCGTTACCGTCATCAACCACCACGTCATCATCTTCC ACAACTTCCTCTACTTCATCAAGTCCTAGATCTCCGTCTCTATCAAAACCACCGCTACCTCCGTCTCCACCGAGAATAAC GACGGTTACGAAGAATCCGTGTTATGGTTGTTATGTTCACCGTAATTCCAGAAATATCTACCTTGTTCACAACGGCAATC ACTGGCAATAACGTGAAGAATCTTCGAATATACGATTAAAAAAAAAAAGAATCTTCGTTTTTTTTTTCTAATGTAAAAAA GAGCGAGAGATTATAATAGAAGGGTGTACG ................................................................................ ..............................i............................................N.... ................................................................................ ............................i.............................................i..... ................................................................................ ................................................................................ ...........................................................i........N........... .............................i.................................................N .......................................................i........................ ................................................................................ ................................................................................ .......................i........N............................................... ......................................................................N......... .............................. Pos Score Pred ------------------------ 111 0.563 Yes 156 0.246 - 269 0.843 Yes 315 0.825 Yes 540 0.588 Yes 549 0.449 - 590 0.557 Yes 640 0.307 - 696 0.554 Yes 904 0.731 Yes 913 0.363 - 1031 0.035 -

Explain the output -- Processing locus 912: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCACGTTTGAATAGTTCGTCTTGTTCAATATCTGAATGCAAATAGAGATAAGCTATTTCGAACACTCATTGTTGAAAAAT CAAAATAATTCATCTTCTTAGAGTATTGTGTGTTCTTACAGAACCCAAGTTTTAAGCATCTGTGTTTCAATTACCTGGCT TACATGTTCTGTCTTGATGATAAATATTGGTTTTGTGATTTTAGTGAGTTGCAGGATTCATATTCTTGTTTTGAAATTTG TGATAATTGCAGTGAATTTTTATTACTGGAGAAGTAATAAGGCAAAGATGGTGAAAATGACATTGATAGCTCGTGTTACT GATGGGTTACCTCTAGCTGAGGGGCTCGATGATGGACGTGACTTACCGGATTCAGATATGTATAAACAACAGGTCAAAGC TTTGTTTAAGAATCTGTCCAGAGGTCAAAATGACGCTTCAAGAATGTCCGTTGAAACTGGCCCCTATGTTTTCCATTACA TCATAGAAGGACGTGTTTGCTACTTGACAATGTGTGACCGCTCTTACCCAAAGAAACTCGCTTTCCAATACCTGGAAGAT CTCAAGAATGAATTTGAACGTGTCAATGGGCCTAACATTGAAACAGCTGCTCGGCCTTATGCCTTTATTAAATTTGATAC ATTCATACAGAAAACCAAGAAACTGTACCAAGACACTCGTACGCAACGAAACATCGCCAAGTTGAATGATGAACTCTATG AGGTTCATCAAATAATGACCCGGAATGTGCAAGAAGTCTTAGGTGTTGGTGAAAAGCTGGACCAGGTGAGCGAGATGTCG AGCCGGTTAACATCTGAATCTCGTATATATGCTGATAAGGCTAAAGATTTGAACCGTCAGGCTTTGATCCGAAAATGGGC ACCAGTCGCAATTGTGTTCGGTGTAGTCTTCCTCCTTTTCTGGGTCAAGAACAAGCTATGGTAAAAAAAAAAGGAGGAAT CTAAGGCTATTTTCGTAATTTAGCGGACTTCTCCAGACATATGTCGACCTCCCCTACCGGACTCAAGTCTCAGATTCCGG CAC ...................................N............................................ ................................................................................ ...N............N............................................................... ...............................................i........i....................... .............................i.............i.....................N.............. .............................i.................................................. .......N.................N................................N..................... .................................................................N..N........N.. ..............N.........N.................................................N..... ............................N.............................................i..... .........................................................i...................... ........................................N....................................... ... Pos Score Pred ------------------------ 36 0.232 - 164 0.149 - 177 0.355 - 288 0.816 Yes 297 0.583 Yes 322 0.301 - 349 0.182 - 352 0.323 - 378 0.722 Yes 430 0.710 Yes 444 0.805 Yes 466 0.236 - 510 0.660 Yes 568 0.344 - 586 0.305 - 619 0.172 - 706 0.462 - 709 0.467 - 718 0.079 - 735 0.432 - 745 0.300 - 795 0.474 - 829 0.312 - 875 0.675 Yes 938 0.621 Yes 1001 0.206 -

Explain the output -- Processing locus 913: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGAGACTTTTCAAGCCGGCTAATATGCATCTCTCCGTCACAAAACGCTGCCGTTTAAGTGTAAAGGCTTTTAATTTCAAT CCACTTAAGCCCAATGGTTAGAATATGGGCCTTTTCTTTATGAATTTGCTAAAATCCACGTGGACGGTAGAGATGCTACG ATGCAGAGAGCGGTCTATGGTGAGGGTTATTACACGAGATCATGACGTTAACACGGGAAACAGGTTGCTTCAAAAGAAAT CAATTCATTCGTACGGGCCTTTAATCCACGCGCCTTCTTCGCATCCCCACGCGCAGTCGTGTGTGATGATGGCTAATCCT CCAACGTTACCGATCTCAGACCATTCCGGTGGTGGTTCCCAATCTCAGCAACCCGTATCAACACCGGCGTTTCGCACGTT CCTCTCTCGCCTCTCGTCGTCAATCCGCCAAAGCCTATCGCAGCGGCGTCCATGGTTAGAACTCGTAGATCGAAGCGCAA TTTCACGTCCAGAGTCTCTCACCGATGCGTATTCTCGGATCCGGAGGAATCTCCCTTACTTCAAGGTAAACTACGTGACG ATCGTCTCCTTGGTGCTCGCGCTCTCGCTCCTCTCCCATCCTTTCTCTCTTCTCGTTCTCCTATGCCTCTTCTGCGCTTG GATCTTCCTCTACCTTTTCCGTCCGTCAGATCAACCTCTGGTCGTCCTCGGCCGTACGTTTTCAGATCGTGAGACGCTTG GTGTTTTGGTAATTTTGACAATCGTCGTCGTCTTTTTAACCAGCGTTGGGTCTTTATTGACCTCTGCTTTGATGATTGGT TTCGGAATCGTATGCTTACACGGCGCGTTTAGAGTCCCGGAGGATCTTTTCTTGGATGATCAAGAACCTGCTAACACCGG CTTATTGTCTTTCCTTAGCGGAGCCGCCACTTCCGCCGCAGTTGCCGCCGCATCAACTCCTGCGTCAGGTCGTGTTTGAA GGACTCTCTTCTCTGTAGACAACAAACTCAATTACTATGATTCTTTTATTGTATTTCTGAATGCGACTATGTAAGGGGAA ATGTAGAATTGATCTTCT .......................N........................................................ .............N..........N..............i................................i....... N...............N........................i...................................... .................................................................i..i........... ................................................................................ ...................................................N............................ ........................N....................................................... ..............................................................N................. ................................................................................ .......................................................................N........ ...........N...........................................N........................ ................................................................................ ....................................N.......................N.......N........... N................. Pos Score Pred ------------------------ 24 0.451 - 94 0.203 - 105 0.294 - 120 0.598 Yes 153 0.501 Yes 161 0.397 - 177 0.343 - 202 0.689 Yes 306 0.734 Yes 309 0.543 Yes 452 0.328 - 505 0.178 - 623 0.395 - 792 0.447 - 812 0.117 - 856 0.194 - 997 0.057 - 1021 0.051 - 1029 0.074 - 1041 0.057 -

Explain the output -- Processing locus 914: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATTAATTGGAGGTGCATTTAATACAACATTCCATAAACGTACGAAGAAAAGTAAGACAAATTAATTCCTTTATTATTA CATAATCATGAACGACTATAAATCCCTCGACCAAGGAAAAACCTAATGATATTTTCATCTCTCCCCTCTCGGTTAGTCAT GAAAGATTTGTTCATGGAAGGAGAAAGAGAAACTTCATCGATGACTTGCTTGACGCCCAAGGATTCTGTTCAAAGCCCTA ACATGTTGGTTAGACAACCAAAAAAGGAGACAACAACACAAACCCCTAAAACGACACGAGGAAGACAGAAGATAGAGATC AAGAAAATCGAGGAAGAGACCAAGAGACAAGTAACGTTCTCGAAACGCCGTCGTGGGCTGTTCAAGAAATCCGCTGAATT GAGCGTTCTCACGGGCGCAAAAATCGCCGTCATAACGTTCTCTAAATGCGATAGGATCTACAGATTTGGCCATGTAGACG CATTGATAGACAAGTATCTTCGTAAGAGTCCGGTGAAGTTGGAGGGATATTCCGGTGATAACGCGGCGGATGAGGAAAGT AGGAGACCGTGGTGGGAGCGTCCGGTGGAGAGTGTGCCAGAAGAGGAGCTTGAGGAGTACATGGCAGCATTGAGTATGTT GAGAGAAAATATAGGGAAAAAGATTGTGGCGATGGGTAACGATCGGACGGTTGATATGGTTCCGGCATGGCCAATCAATG TGATGGGATGGAAACCAACGATGGATATGCAAAAATTGGAAAATCTGACGGATGGGGTTAATCGATGCCGTGTAGGTCAA AACGGTGATTGATTAGTCCAGTCGTGAAGTATTCCTCTAATGAATTTCTAAAATATTTTATTTAGTTTTGTTAAGTTTAA TTATATGATTTTATGCTATTGATCATCAGAT ................................................................................ .......N.....................................N................................N. .............i..........................i....................................... ..i............................................................................. ................................................................................ .............................................N.........................i........ .....................................................................N.......... ............................................................N..............i.... ...............................i.......................N..........i..........N.. ..N....N............N.....N........................N............N............... .......................................N........................................ ....N.......N.................. Pos Score Pred ------------------------ 88 0.424 - 126 0.339 - 159 0.232 - 174 0.832 Yes 201 0.679 Yes 243 0.650 Yes 446 0.298 - 472 0.526 Yes 550 0.249 - 621 0.347 - 636 0.616 Yes 672 0.569 Yes 696 0.484 - 707 0.575 Yes 718 0.309 - 723 0.224 - 728 0.477 - 741 0.304 - 747 0.163 - 772 0.246 - 785 0.123 - 840 0.050 - 885 0.049 - 893 0.049 -

Explain the output -- Processing locus 915: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTCTCTCCTACAATTAAGCTCTTTGACGTACGTAATCTCCGGAACCAACTCCTATATCCACCATTTACTCCACGTTG TCTCCAATTATTGGACGTTGAAACTTGACACAACGTAAACGTATCTATGTGGTTGATTGTATGTACATATGTACAAACGT ACACCTTTCTCCTCTTTCACTTCATCACTTGGCTTGTGAATTCATTAATTCCTGCGAAATGGAACCTCGAATCAATGACC TTCAGGTGGAATCTCGGGTTCATGAGTTGCTCGATTTTCCGGTTCATACAAACCAGATATCCTCTGCAATCTACGAGTGT CCCAACGACCACATCGAAAACCCTAAGAAGAAACCGTACAATTGTCCACACTCAGGAGCCAAGTGTGATGTCACAGGAGA TATTCAAAGGCTACTACTGCATCTAAGGAATGATCACAATGTTGAAATGAGTGATGGGCGTAGTTTCAGTCACAGATATG TTCATCATGATCCTAAACATCTTCATCATGCTACTTGGATGTTAACACTTCTCGATTGTTGTGGTCGAAAATTCTGCTTG TACTTCGAAGCGTTTCACCTGAGGAAAACACCAATGTACATGGCCTTCATGCAGTTTATGGGAGATGAAGAAGAGGCAAT GAGCTTCAGCTATAGTTTGCAGGTCGGAGGGAATGGCAGAAAGCTGACATGGCAAGGCGTGCCGAGGAGCATTCGTGACA GTCACAAGACGGTTCGTGATAGTCAAGATGGCTTAATCATCACTCGCAAATTGGCTTTGTTCTTCTCTACCGACAACAAC ACCACCGACAAGGAGCTGAAGTTGAAGGTTTCAGGTCGTGTATGGAGAGAACAGCCTGTTTCGATATGAATTCGATAATT AGGTTACAGAATTGGACAACTATAGAATATTTGGTGACAAAAATATCTTTTCTTGATTTAAAAATTAAATATAGCAATTT CGAGGGAA ................................................................................ ..............................................N.............N.......N........... ..........................................................i...............N..... .....................N.......................................................... ...................................................................N............ .............................N........N.......i......i.......................i.. ......N....................N..........N......................................... .................................i.....i........N........N......i.............i. ................................N...............N............................... ...........................N.................................................... .........................................N.......................N.............. ................................................................................ ........ Pos Score Pred ------------------------ 127 0.310 - 141 0.272 - 149 0.406 - 219 0.711 Yes 235 0.315 - 262 0.278 - 388 0.306 - 430 0.140 - 439 0.295 - 447 0.506 Yes 454 0.501 Yes 478 0.523 Yes 487 0.211 - 508 0.228 - 519 0.171 - 594 0.624 Yes 600 0.616 Yes 609 0.285 - 618 0.404 - 625 0.663 Yes 639 0.591 Yes 673 0.471 - 689 0.273 - 748 0.358 - 842 0.283 - 866 0.233 -

Explain the output -- Processing locus 916: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATATTTAACTTATTTTATGCACAGAGATCTTTAGTATAATTCATGAAAATATGGTTTTATCTTCCATGATCTAGGGGAAC TAAAACATTTGTAAAGTTTGAGCCCATATTTTGTGTTTGTTGCTTTTCAGAGATGCATATATTATGGTTGTTGTTCTGTT TATGAATTGTTATGTGCATTGTTTTGCAGCAATGGCGCCACCGATTGAGGTTTCTACCAAAAGCTACGTTGAGAAACATG TTTCACTTCCTACTCTTAATGAGAGGATACTTTCGTCCATGAGTCACAGATCAGTAGCTGCACACCCATGGCATGATCTC GAGATAGGACCTGAAGCCCCAATTATCTTCAATTGTGTGGTTGAGATAGGAAAAGGGAGCAAGGTGAAATATGAACTCGA CAAAACTACGGGTCTCATTAAGGTCGACCGTATTCTTTACTCATCTGTCGTATACCCACACAACTATGGGTTCATTCCGC GTACCCTTTGTGAGGACAGTGACCCTATTGATGTTCTTGTCATTATGCAGGAACCGGTGATCCCAGGATGCTTTCTTCGG GCCAAAGCTATTGGTCTGATGCCAATGATTGATCAGGGTGAGAAAGACGACAAGATCATTGCTGTCTGCGCTGACGATCC AGAGTATCGCCATTACAACGACATCAGTGAGCTTCCGCCTCATCGTATGGCTGAGATCCGCCGTTTCTTTGAAGACTATA AGAAAAACGAGAACAAGGAAGTAGCCGTTAACGACTTCCTTCCGGCAACTGCAGCCTACGACGCAGTTCAGCATTCCATG GATCTCTATGCAGACTACGTCGTGGAGAACCTAAGACGTTGAATCACCACCTCAAGCAATGGAATGAAAGCTCACAACTG CATTATTCACAAATATAAATATACATATAAATGTCAGGCTTCTCAAAGGATATATTTGTTG ................N.........................N.......N..............N.............. ....................................................N..........i................ ..................N...................i............................i....N....... ......................................................................N......... .................................................................N.............. ..............................N.............i......................i............ ..................i.....i....................................................... ..............................................i................................. .............................................................................N.. .......N..................................................N....N................ ..............................N.............................. Pos Score Pred ------------------------ 17 0.180 - 43 0.140 - 51 0.316 - 66 0.147 - 133 0.224 - 144 0.682 Yes 162 0.338 - 172 0.066 - 192 0.643 Yes 238 0.518 Yes 259 0.151 - 279 0.653 Yes 308 0.522 Yes 313 0.270 - 391 0.493 - 466 0.494 - 511 0.325 - 525 0.540 Yes 548 0.659 Yes 579 0.713 Yes 585 0.763 Yes 687 0.849 Yes 798 0.280 - 808 0.140 - 859 0.173 - 864 0.230 - 911 0.074 -

Explain the output -- Processing locus 917: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTTAAACTTGACAAAATCTCTAAATGTAAAGTCTAATATAAACCTTAAAATCTAAAAACTAATTATGCTTGTTTTTGA GAAATTTTTAAAAAATTGTGAAAAAGAAAAGTAATGAAAAAAATATTGAGAGTGAGTGATTGGAGGAAAATAACACAACG CAACAATCTAACAAAGAGAGACCTTATCATCATCAGCATCACCTTAACCCATATAACATTCCTCTTCGAAAATGCCAACA ACAAATTTCATAGAGAAGCAGAAATGGCGAAATCACTCCTTTGTTCATCAACCTTAAACCCTTTCTTCTCCACCACTCTC TCCTCTTCGAAGAAGAACCAAATTGCATACTCGGGAAACAGCAAGAACCAGACATCGTCGTCTCTGTTATGGAAAAGAAG GGAACTGTCGCTTGGATTTATGAGCAGCTTAGTTGCGATTGGTCTCGTGAGTAATGACAGAAGAAGACACGACGCGAATG CAGCGATTCTTGAAGCTGATGACGATGAAGAGCTTCTCGAGAAAGTGAAGCAAGATCGGAAAAAGAGGATTGAGAGACAA GCTGTTCTTAATTCTGCTGTCAAGGAAAAGGGGTATTTGCAGGATCTTGTTTACAAGTTGAGCAAGGTAGGACAAGCCAT TGAGAACAATGATCTACCAGCTGCAGGTTTGGTTTTGGGGAAAGGCATTGATACCGAATGGGTCAAAACCGTTAATCTGG CTTTCACAAAGTTGAGTACAAGCCCAGAAGAGAATACAGAGGTGGAAGCATTCAATTCATCATTGGCTTCTCTTATCACA TCAGTGAACAAGAACGATATAGAGTCATCGAAGCTCGCTTTCGTTTCATCAGCTGGTGCATTTGAGAAATGGACTACTTT GACAGGTCTTTTGGAACAGCTTAAGGGACTGTGATAGGCTGGCTACAAATTCTTTACTCTTTTTTTGTATTTTCCAACAG ATTTGTCAAAGAGGGTCTTTTTTTTCTCCAAATAATCCTTTGAATAATCATCA .........................N........................................i............. .................................N.............................................. .......................................................................N........ .......................i........................................................ ....................................................................i........... ...................i.................................N.......................N.. ..................N.....N....................................................... ................................................................................ ........N................................................N...................... ................................................................................ ....................................................................N........... ................................................................................ ..................................................... Pos Score Pred ------------------------ 26 0.291 - 67 0.589 Yes 114 0.415 - 232 0.408 - 264 0.801 Yes 389 0.507 Yes 420 0.744 Yes 454 0.232 - 478 0.244 - 499 0.114 - 505 0.124 - 649 0.173 - 698 0.390 - 869 0.400 -

Explain the output -- Processing locus 918: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATAAAATCCCAAGAGTCACTTTCTATATTCGACACATTAATATTGATTTTCTTCCTAATCCAAATTACGTTGACCATAT CATACATTAATTATTTACTAGTTGGAATTATATAATATGAAAAAATTCCGAGTCACTTTAAGCTTTTCGAGGTATCTCCT CCGCGCGACGATGTCAGAGACTCGCCGGAATCAGTCTAGCGATGAACAAATCGAAAAATCAGAGATTCAGCACCAAAGAG TTGTAATTGAGAACAGTCATGGTGAGAAACTCGTAGGAGTTTTGCATGATACTGGTTCTACAGAGACTGTTGTCATCTGC CATGGATTTCGTTCATCAAAGAATCGGATTCCAATGCTGACCATTGCAAGTTTCTTTGAAAGAGCAATGATCAGTTCTTT CCGGTTTGACTTTGCTGGCAACGGGGAAAGCCAAGGATCATTTCAGTATGGTAACTATCGCCGTGAAGTTGAAGATTTGC GCTCTGTTTTGCAACACTTGCGTGGTGTTAACCGTGTGATTTCTGCGATTATCGGACATAGTAAAGGTGGAAATGTGGTG CTTTTGTATGCAGCAAAGTACAATGATGTGCAGACTGTTGTGAATATCTCAGGGCGGTTTTTTCTTGACAGAGGAATCGA ATTTCGGCTTGGTAAGGACTACTTCAAGAGAATCAAAGATAATGGATTCATTGATGTTAGCAACAGAAAGGGAAAGTTTG AATATCGGGTAACAGAAGAGAGTCTAATGGACCGTCTCACAACCAATGCTCATGAAGCATGTCTCTCAATTCGTGAAAAC TGCAGTCCATGGGTCAAATGATAGGATTGTGCATGTAACAGAAGCGTCCGAATTTGCTAAACAGATAAAGAATCATAAGC TGTATGTTATTGAAGGAGCTGATCATGAGTTCACTTCTCA ................................................................................ ....................................i........................................... ..........i..............................N...................................... ..................N..........................N.................................. ...............................................N................................ ........................................................................N....... .......N..............N..N...................................................... .........................................N...........N.......................... ..........................i..................N.....i......N..................... ........N........N..............N............................................... ...N....................N............... Pos Score Pred ------------------------ 117 0.611 Yes 171 0.747 Yes 202 0.139 - 259 0.406 - 286 0.165 - 322 0.431 - 354 0.770 Yes 387 0.504 Yes 448 0.229 - 553 0.457 - 568 0.280 - 583 0.362 - 586 0.280 - 682 0.188 - 694 0.195 - 747 0.691 Yes 766 0.359 - 772 0.678 Yes 779 0.388 - 809 0.098 - 818 0.118 - 833 0.107 - 884 0.033 - 905 0.058 -

Explain the output -- Processing locus 919: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAACATTTTCGCATAAGATAATAACATATACTTATATTTTTTTAAGAACTAATTTGCCAATCAAAAGTAGCAATAAAG TGAAGAAAAAAAGTCTAATAAAGTGTAGAAATTAATCAAATTTTAAGTCCTAAAGTTGGAAAAATGGTAAAGAAGCACTC AAAAAAAAAAAAAAAAATTGTGTATGTGTGGGCCGAATTTGAGAAACCAGTGACACCAATAAAAACCTCATCTTTCAATC TCACACAAGTATTTGTGCCTACTCATTTCCTCCAAACGTCAAACATAGCAGCAGCCATGGCTGCCACCAACACAATCCTC GCATTCTCATCTCCTTCTCGTCTTCTCATTCCTCCTTCCTCCAATCCTTCAACTCTCCGTTCCTCTTTCCGCGGCGTCTC TCTCAACAACAACAATCTCCACCGTCTCCAATCTGTTTCCTTCGCCGTTAAAGCTCCGTCGAAAGCGTTGACAGTTGTTT CCGCGGCGAAGAAGGCTGTTGCAGTGCTTAAAGGTACTTCTGATGTCGAAGGAGTTGTTACTTTGACCCAAGATGACTCA GGTCCTACAACTGTGAATGTTCGTATCACTGGTCTCACTCCAGGGCCTCATGGATTTCATCTCCATGAGTTTGGTGATAC AACTAATGGATGTATCTCAACAGGACCACATTTCAACCCTAACAACATGACACACGGAGCTCCAGAAGATGAGTGCCGTC ATGCGGGTGACCTGGGAAACATAAATGCCAATGCCGATGGCGTGGCAGAAACAACAATAGTGGACAATCAGATTCCTCTG ACTGGTCCTAATTCTGTTGTTGGAAGAGCCTTTGTGGTTCACGAGCTTAAGGATGACCTCGGAAAGGGTGGCCATGAGCT TAGTCTGACCACTGGAAACGCAGGCGGGAGATTGGCATGTGGTGTGATTGGCTTGACGCCGCTCTAAGTCAGAGGCTAAG CAAGTACTCTTATGTCTACTGTGTTGATCTTGTCTGTTTGAACAGATGTAAGAATCTTAGCAAATAATGTTTGGCTTAAT AAACTT ................................................................................ ...............................................................N................ .......................N........................................................ ........................................................i....................... ................................................................................ ................................................................................ ..........................................N.............................N....... ................N................................N..............N............... .....N...i....................................i.....................N........... N.......................N.....N.....N........................................... ....................................................i....................N...... ....................................i........................................... ...........N.................................N....................N............. ...... Pos Score Pred ------------------------ 144 0.341 - 184 0.241 - 297 0.739 Yes 523 0.308 - 553 0.381 - 577 0.288 - 610 0.249 - 625 0.293 - 646 0.489 - 650 0.586 Yes 687 0.840 Yes 709 0.257 - 721 0.107 - 745 0.158 - 751 0.178 - 757 0.249 - 853 0.621 Yes 874 0.139 - 917 0.606 Yes 972 0.059 - 1006 0.058 - 1027 0.054 -

Explain the output -- Processing locus 920: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCAATTAAACCAATCTGAAAAAAAAGTGTGATAACCAATGAAAACTCGGCCCACTAAACAGGCCCAACAAACAAATAATA AACCCAAGAGGTGCTTCTTAATCGCCGATGCCTCTCTTCATCAAAATTCAAAATCACTTTTGTCTTTTCAAAAATCACAG CAAAATAATATTCAAATTCAAAATCTGTCCAAATTCATCTAGGGTTTCTGATAAAACAGACGAAGAAGATCCAAATTCGC TAACAATGGGAGGTGAAGGAGAAGATCTAGAGCCACTTTTTGATTATCGTCGTGTTCAGCCAGCGAATTTCGTTTGTATC GATGATGATGATGATGATGCCTCGGTGACGCCAATTCCGAAGAAAGCGAAAACTTCACAAACAGTTGAGAAGTTAGATGA TGATGTGAAAGTGATTGAAGTTACGGGTGATGACGATTGGTTGCTTCCTCCACCAAAGGTTATCTTCGACAAAAGTAAAG ACTCTGTTGAAGATTCAACTATTAAAGCATTGAGGTCGAAGAAGATGGAGCTCATGTCATTTACAAAAACTGTAGGAGAT GTGATGCAGGAAGTTGAGGAGTCTGCTAAAAGGGAAGTTGAAGAATCTCGCAATCCATCTTCAGAGACTGCTGCTCAGTT ACCATCAGAGCCTACGAATGACAGGGCCAAGATTGTTATTACAATACAGGACAAAGATGGACAGAAGACACTCCGGGTGT TTGCGGACGAGAAATTTGAGAGGGTTATCAAACTGTATACAGATAAAGCTAAGCTTGACCCGCAAAATCTTGTATTTATT TTTGACGGTGATAAGATTGATCCATCAACCACTCCTTCTGAACTTGGCATGGAGGATCATGATATGATTGAAGTGCACAC CAAAAAAACTTGACAAATAGGTAACTTATGAAGTCCTCCACATCTTTCTTCTTGCTCTTATTGTGATATGATGATAACTC ATTATCTTCGATTGAATTCAAGTCAGGCGCTA .....................................i.......................................... ...........................N.................................................... ................................................................................ .....i.......................................................................... ..N..........................N.................................................. ............................................i........i........................N. ...i............................................................................ .................N......................................N....................... ................................................................................ ................................................N.........N....i................ ...........................N.......................................N..N......... ................................ Pos Score Pred ------------------------ 38 0.546 Yes 108 0.223 - 246 0.838 Yes 322 0.056 - 325 0.099 - 328 0.146 - 331 0.073 - 334 0.119 - 337 0.091 - 397 0.215 - 400 0.199 - 403 0.111 - 430 0.112 - 525 0.613 Yes 534 0.744 Yes 559 0.311 - 564 0.589 Yes 658 0.238 - 697 0.221 - 849 0.317 - 859 0.185 - 864 0.542 Yes 908 0.159 - 948 0.040 - 951 0.054 -

Explain the output -- Processing locus 921: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGACGGGGAGAGAAGAAAAGGTTTTTGAGAGGTTTTATACAAAACGCAGTCGAAAACCGGAGAAGCCTAATCCGTTTTA TGTATAATTTGATTCGGTTAAGAAAACCGAACCGGGTATGATGAGATGTGGTTTTTGATTAGGCTCCACGATTTGATTTG GTCGCACCAAAGATCGGATTTTTATCAGACGGAAAGGAGGAGACGGAAGAAAATGCCGGTGAGAGTTGTTGAGAGCAACA CGCCTGCTCAAGTTTCAGGAACTGATCCTGGGAATCGATCACCACTTCCCCCTAGTTCACTTCTCGGCGCTGGCCAGGCA TTTTCTGGTACGCAAAATGTCTCTAATCAACAGAAGGAGGAAGCTTGGAGAGTCAACGTCCAGATACAGGGTATTGACCT TGAACATGGTTATCTTTGTGGCACCATGGAAGCTCTAAACGTTCCCATGGCAGACACTCCTGTCATAACATTCTGGGAAG GGGAAATCGTTGATGGAAAGAATTATACCTTTTACACCGGAAAATGGGAAGCAACGAGGGAAGATGATATGAGACATTGG TCAAAGTTCCCGTCTTTTTCGCCTCTTCAGGGACAAGTTGAATCTGATGGTGGCAGGCAATTGGATCTTAACAATTATCC TTATATATTTATGAGATGGAAAGAGCAATACTTTGTAAACGTTGGCACAGATTGTGGACTAACAATAGCTGGATTTTACT ACGTATGCTTCTCCTGCAGCGATGGTTCCATCAGCGGTTTCTATTATGATCCAAACAGCAGTCCGTTTCAGAAGCTGGAG CTGAAAACAGTTAACGAAGGAAGATCTGGTTTCAGCTTTTCTTCTTACGAGTTGCAATGATGAATGCTACCAAAATCATC AGACAGCGCCATATGATACTCTCGTCTAAACATGCGATGCACGTGATCCAGTACATTATGACGTGTCAATTATTATAAA ...............................................................................N .....................................N..N....N.................................. ....................................................i........................... ................................................................................ ................N............................................................... .....N...................i....................i................................. ............N..............................i...................N....N........... ..............................................i................................. ..........N....N................................................................ ....N................N.......................N.................................. ........................................................N..N...N................ ............N..................N....N....................N..................... Pos Score Pred ------------------------ 80 0.131 - 118 0.330 - 121 0.414 - 126 0.399 - 213 0.838 Yes 337 0.487 - 406 0.357 - 426 0.641 Yes 447 0.839 Yes 493 0.334 - 524 0.615 Yes 544 0.222 - 549 0.286 - 607 0.541 Yes 651 0.345 - 656 0.294 - 725 0.269 - 742 0.266 - 766 0.261 - 857 0.226 - 860 0.251 - 864 0.107 - 893 0.032 - 912 0.073 - 917 0.084 - 938 0.050 -

Explain the output -- Processing locus 922: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCTCTGTAAAACCTTTTCTTTCATGCTTTAAATTGAATTATCATTTAACAAAAATAAATAAAAACAAATTGGGCCTTGAC CTATTCACAAATTGGGCGTAAACACACATGAAGGTATATAAATACAGAGGCGTTTGCTTTTCTGGCCATTCCACATTTTT TTATATTTCTCTCAAATTTGTTCAAGAATCCCTTTGTTTCTATCCAAGAATGGACGAATTTGAAGGATTGAAGAGAGAAG TTGAGAGACTTTTGGATATGGTAGATTCTGAGAACAGCGATTGTGAGGAAAAAATAGCAATACAAGAAGAAGTTGGCGAT CTTCCGAGTAGTTCTGTGGCTGTGGAGTTTCTCGAAGACGAGATCGATAGAAAGGAAGAAATCCTTCTTGCTGCGAGTTG CACGCTGCTGAACATGATTAGCGGACTTGATCACTCCTTTGATGGCAATGAAAGAGATATGGGACGACTACAAGTTGAAG AATTCAGAGCCGCCTGTCTCGGCCACAAAGTGTTGGTGAACGAAACGGAGGAGCAAACTTTCGAGAGAGCGGATTTGATT AGAACTCTGTGGCAAAAAAAAATTCTCGACTCTGTGCGTTTGGCTTACTTACAAGGGGAAAAGGAGATGCCGTTTCGTCC CTACGATCAAACCGAATTAGAGGCACTCAAAACGGACTCGGGTGAGAAGGTTTACAGGCTTGTTAGGAAGGGCTTTCGTG AAGCGCGAGTGGCTGTGCGGACGAGTGTTGATTTCAAACCATGGGATCTTGAAGAAGGAAGAGAATGTACTCTTTCCGAG TTACTAGATCAGCTTCAAGCGTTAATCAGGGGGCGTATAAGACGTCATGCCTGAATTCACAGGTTTTATTATTTTATAAA CAGTTTAGGATTTGAAAAAGACTTTCTTGATCCTCTGTTCTCTGATCGAAAGTACGGTTTAGGATATGAATTA ......................N......................................................... ...........................N.................................................... .................................................i.............................. .................i.............................................................. ................................................................................ .............N...........................N.....N..........i..................... ................................................................................ ..................................................................i............. ................................................................................ ........................................i.......................N............... ..............................................i................................. .................................................................N....... Pos Score Pred ------------------------ 23 0.268 - 108 0.466 - 210 0.756 Yes 258 0.661 Yes 414 0.304 - 442 0.360 - 448 0.127 - 459 0.721 Yes 627 0.603 Yes 761 0.522 Yes 785 0.186 - 847 0.545 Yes 946 0.052 -

Explain the output -- Processing locus 923: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAGAATTGCAAATTCCAATTCCAAAAACTAAACTAAACCAAACAAAATTTTTATTAAAAACAAAAAAATGTTGGTTGCT TAAAGTTTAGCAATTAAATAACACACTTTATTGTGGGCACATGATTCACCCTTAATGCCCTGTCGCTCACTCTTTATTAG CTACTACCAATTAAATCTCTCTTTTTCTTCTCTTACCATTTTCACATTTTCGAGTTTTACACCAAAGCCTCTTCACTTTC TCTCTCTCTCTACTATAGTCTCCTACTACCTACTACTACTTCTTCTCTCTCTCTTTCATTCACTACATAAAAAGCTTCGA GCTCCCATCACTATATACCCTCCAAAGATGCAATTTTCAAGAAACTCAATCCTTAGGCAACTAAGCCGAAAAGAAGGTTG GAGATCGGCTTCTAAAAGGTGGACTTCCGGAGATAGCTCAACAGCCTTCAACGACGACACAAGTGGTGGTGGTTACTCAT CAATGGAGGGTCTTTATGGAGTTTACTCCGGTGGAGACACGGCGGCGAGAAGTAAGAGAGTGATGGTTGTAGTTGATGAA AGTTCGAGGTCTAAGCATGCTATGATGTGGGCTTTGACTCATTTGACTAACAAAGGAGATTTAGTGACTCTTCTTCATGT TGTGTCTCCTGATGATGAAGCTACTCCTTCTTTGGCTCAATCTCTTGGTTCTCTTTGCAAAGCTTGTAAACCCGAGGTGG ATGTGGAGGCATTGGTGATTCAAGGACCAAAGCTAGCAACAGTGTTAAGCCAAGTAAAGAAACTTGAAGTCTCTGTTCTT GTGTTGGGTCAGAAGAAATCTGCACCACTCATCTCTTGCTTATGTGGACCTAGCAGATCAGAGGAGCTCGTGAATCGTTG CATCAACGGTGCAGATTGTTTAACGATTGGTGTGAGAAAACAATGCAAAGGTGTTGGTGGGTACTTGATCAATACACGAT GGCAGAAGAATTTCTGGCTCTTGGCCTAATCACAATTCTAATCTTATTAGATAATTTGTGTTAATTCTGTGTTTATTGTA GTTTAATAAACTCTATGTAGTCTCTTTGTTGTCTTATGTTAACCTAAC ....................................................................i........... ........................................N.............N......................... ................................................................................ ................................................................................ ...........................i.................................................... ................................................................................ ..i............N..............................................N............N.... ................N....i..i...................................................N... ...........N..N................................................................. N............................................................................... .........................................N...................................... ..........................................N...................................N. ................................................................................ ..............N....................N............ Pos Score Pred ------------------------ 69 0.689 Yes 121 0.338 - 135 0.227 - 348 0.679 Yes 483 0.841 Yes 496 0.132 - 543 0.448 - 556 0.051 - 577 0.082 - 582 0.621 Yes 585 0.674 Yes 637 0.216 - 652 0.159 - 655 0.309 - 721 0.084 - 842 0.185 - 923 0.425 - 959 0.431 - 1055 0.061 - 1076 0.044 -

Explain the output -- Processing locus 924: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACCTTGGATCACTCTTCAAGCCCGATTTTAATCTTGGTAATATATACATTAATATATAGACACAAACATATTTTGTGTT TTTTCAATCGTAAAATATTGATACATATACGCATATGTAATATATGAATCGACGCGCATGTATTTATTTACAAGTTATTC AAGAACTTTCTTATTAAAGTGTAATATGTTTGCAGATACATGTGGTTTCATGAATAGATCGCCGGAACATATTTTCGGAT CACCGGAGATTGATTACTCCGACGTCTCCACAGGTTACCTCGAAGACGCACTTATCGAGTCCGGTGAGAGAAGCAAAAGG AGACGTCTCTTGTTCGAGGATCCGTCCAAATCTTTTAACGATGATGATTCGCAGAACGATTGGGGCCTGCATGAGAGTTA TAGCTGTTTGAATAGTCAGTTTGTCACTCCACATGTTAACACAGGTGAACGTATAAGTGGAGTCAGTTACTGTCAGGAGA CGATATCGAATGTTTACGAGTCTCCCGACACTTCTGTCTCTTATGACAAAATTTATGTTCGAGAAAAATCTCCAACCGAA CCATCCTCATCCAACTGTGGTAATAAGAACAAAAGACTAATCACGAAACTAGTGTACCCATTTGGATTGGTGAAACCAGG TGGTCGAGAAAATGATGTAACCCTAAATGATATCAACGAGAGAATTTTGATGGCTCCATCTCGACCGATTCGACATCCGG TAGGAGACTTTGCCTCTCGACCATGTGTCTCTGGCCGTGGACCGGGCCTCTCCGGAAAAGCTGTGGTGGCTCTTACTAAG ATCCAAACACAAGGGAGGGGTACAATTACTATAATCAGAACTAAGGGGTAAACTGTGTTATATTGGTAATTAGTTGGGGC TGTTATATATGTGTAACATAAATGAGGTTTTAGAAAGCTAAAGTGATCATTGGCTATGAGAGAAACTTTC ................................................................................ ..................................N........N.............N...................... .........................i.............N.........i.............................. ................................................................................ ........................................i..N..........................N......... ................................N............................................... .........N................................N...........N......................... ................................................................................ ...........N..N...........N......................i.............................. ......................N......................................................... ................................................................................ ........N............N.................................N.............. Pos Score Pred ------------------------ 115 0.288 - 124 0.319 - 138 0.120 - 186 0.689 Yes 200 0.451 - 210 0.574 Yes 361 0.707 Yes 364 0.257 - 391 0.102 - 433 0.144 - 490 0.387 - 523 0.137 - 535 0.226 - 652 0.332 - 655 0.421 - 667 0.470 - 690 0.789 Yes 743 0.443 - 889 0.081 - 902 0.101 - 936 0.066 -

Explain the output -- Processing locus 925: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCTTGCCATGGACATTCGACTTATCAGTATTTTTGTACATTTTGGTTAATGTTTATCGAATTGGTTTGATCAGTCTGTG GATTTTAACTTTACAAGTTAAGATGCTATTCTTTTAGGTCTAGTTGATAGGTCATGGATTTGTTGTTATATGGAGTCTAG ACATTTTGCCACTGCTTTAGTGTTCTTAGCTTCTAAGGGTTTCGTTGATATATTGTACTGGGTTTATCGTTTCCAGGCAT CTAGTATGGCTTTCTGCACTAAACTTGGCGGTCACTGGAAACAAGGGGTAAATGTTCCAGTGTCATCAATGCTCGGTTCT CTTCGCTACATGTCCACAAAACTTTATATTGGTGGCCTGTCCCCTGGAACTGACGAGCACTCCTTGAAGGACGCTTTCTC TAGCTTCAATGGAGTGACAGAGGCAAGAGTCATGACAAACAAAGTGACCGGGAGGTCTAGAGGTTATGGATTTGTTAACT TCATAAGCGAGGATTCTGCCAACTCTGCTATTTCAGCAATGAATGGACAGGAGCTGAATGGGTTTAACATAAGTGTGAAC GTTGCAAAAGATTGGCCAAGTTTGCCATTGTCTTTGGATGAGAGTATAGAAGAAGCTGAGAAGAAAGAAAATAAGATGAT GAGCCGATCTGTATGGAAAGATCCATTCGTTGATGCGTTCCTGATGAAGAAGAAGAATGCAGCTCTGAACAGGAAAATAT GGTCAAGGAGATCGACGATTCTTCCCGAGTATGTTGATTCGGCGGTGAGAATCTACAACGGCAAAACTCATGTGCGTTGT AAGATCACAGAGGGGAAAGTTGGGCATAAATTCGGAGAGTTTGCGTTTACAAGAAAAGTGAAGAAGCATGCTAAAGCAAA GTAGCATCTTTGAGAATGAATTGGTCAGCTGCTTGAAAGGATTGTGTTGAAAAGCCAAAAACGAATCAAATCAATAAATT CTCAATAGGTAAAACTCATTAGC ........N........................................N.............................. ......................N..............................N...............i.......... ................................................................................ .....i.............................................N................N........... .........i...................................................................... ........N......................i.................................N.............. ......................................i...N..............N...................... .....................................N.....................................N..N. ............i...................N..........i............N.....................i. ..............................N......................................N.......... ...................................................................N............ ...............N................................................................ ....................... Pos Score Pred ------------------------ 9 0.467 - 50 0.086 - 103 0.166 - 134 0.238 - 150 0.585 Yes 246 0.652 Yes 292 0.227 - 309 0.466 - 330 0.586 Yes 409 0.232 - 432 0.657 Yes 466 0.336 - 519 0.534 Yes 523 0.134 - 538 0.341 - 598 0.106 - 636 0.281 - 639 0.424 - 653 0.538 Yes 673 0.148 - 684 0.656 Yes 697 0.207 - 719 0.715 Yes 751 0.323 - 790 0.156 - 868 0.270 - 896 0.162 -

Explain the output -- Processing locus 926: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGCCAAGAAAAAAAAATCCTTATAAAAACGCACAAGAAAAAAGTAAAAGAGCTATTCTGTAAAATAAAAATGAAAAAAG TATTGAATCCTACATTAATAGATACAGGATATGATGTTCTGTTTCTCAACGGCTACATCTGTCTGTGATCCTTTTTTAAT CCAACGATCCAGATCTCTCCTCATCGCCTATATAAAACTCACTTCTCTTACATCAAACAAACAACACGCTTGGTTAAACT CCATCAGTACTTTAACCATCACTTCAAAGTTCAAACTCTTTTTTTTTTTTTTGTTTGATCCAAATTTTAACAAAATTATG GATCGCAATTTCCTCCTAACAGTTACATTGATCTGCATTGTCGTCGCCGGTGTCGGTGGCCAATCTCCTATCTCTTCTCC GACCAAATCTCCCACCACTCCTTCTGCTCCAACTACTTCCCCTACTAAATCCCCCGCCGTTACTTCTCCCACTACGGCTC CGGCGAAAACTCCAACTGCTTCGGCTTCTTCACCGGTTGAGTCACCAAAATCTCCGGCTCCTGTTAGCGAGTCGTCTCCA CCACCGACACCTGTTCCAGAGAGCTCTCCTCCGGTTCCTGCACCAATGGTTTCTTCTCCAGTGAGCTCTCCACCGGTTCC AGCACCAGTAGCTGATTCTCCTCCAGCTCCGGTAGCCGCTCCGGTTGCTGATGTACCGGCTCCTGCTCCAAGCAAGCATA AGAAGACTACAAAGAAATCGAAAAAGCATCAAGCTGCACCTGCTCCGGCTCCGGAACTTCTCGGTCCACCTGCACCACCG ACTGAATCTCCCGGACCTAACTCCGACGCTTTTTCTCCCGGTCCTTCCGCCGACGATCAGAGCGGAGCAGCGAGCACAAG GGTGTTGAGGAATGTAGCGGTGGGAGCGGTTGCAACCGCATGGGCCGTTCTCGTTATGGCATTCTAAATTATTTATCATT TCACCTCTGATATTTTTTAGTTTTTATATTTTTGACCCTACCATTGTCTCTTTCTTTACGAATTTGGTAATGAGTATTAT TTAATT i.....................................................................N......... ..............................N..N.............................................. ................................................................................ .............................................................................i.. ................................................................................ ................................................................................ ................................................................................ .............................................i.................................. ..................................................N............................. ................................................................................ ................................................................................ ...........N...........................N...............N........................ .....................................................................N.......... ...... Pos Score Pred ------------------------ 1 0.568 Yes 71 0.299 - 111 0.221 - 114 0.256 - 318 0.754 Yes 606 0.901 Yes 691 0.345 - 892 0.134 - 920 0.069 - 936 0.117 - 1030 0.050 -

Explain the output -- Processing locus 927: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCGAAGACAAATAATACAGATAGAAATAATTGTCTTTACAAAACGGGCCTTTAACAGGCCCAATAACCTAAAAAGGCCCA TGGTGTACAATAGAATTCTGACTAATGTGTACCATCATCATCTCTTATCCAAATTTTACTCTCTCATCTCCTCTTTGCTT TCACAATCTCACAACAATGGCGTCTTCGCTGTGTGTATCAAATTCCACAATTTGCCCTCTGCCTAATGTATCTTCGCAGC CGCTGCTATCATTCTCCCACTCTCTCAGGCCATTCATTTCCAAGTCGAAACCTATGTGTGCCTCGATACAGAAGAGAGAT GGCTCTCAATTTGTGGTGAAATCTCAGGCTCTTGACTTCTCTGGAACTTTCTTTGAAGGTGGATTCGGGTCGGATGATGA CCCGACTTCTCCTTCCGGGTCGGGAGTCTCAACTGCCCTTGAAGACAAACCGGAACCTCAGTGCCCACCTGGGCTCAGGC AGTACGAAACAATGGCGGTTTTGAGACCAGACATGTCTGAAGATGAACGGCTTGGTCTTACCCAGAAATACGAAGAGTTG CTTGTGGCAGGAGGTGGAATGTATGTGGAAGTGTTTAACAGAGGAGTGATTCCGTTGGCTTACAGCATAAGAAAGAAGAA CAAAGCTGGAGAAACCAACACTTACTTGGATGGAATCTACCTTCTCTTCACTTACTTCACCAAACCCGAATCAATAGTTC CACTCGAGACCGTTCTCACTGCCGACGATGACATTATCCGATCATCTTCCTTCAAGATAAAGAAGAGGAAGTACAACTGA AGACCATGAACATTGTGTATGTGAGAAAAATGTCAGATTCTTTTTTTGTTTCTTTGTAAGAACAAAAGTAAATCTATCTT TTTTGTATCTTTTTTTGTG ...............................................................................N ........................N....................................................... ................i................................................i.............. .....................................................i........................i. .........................................................................N..N... ................................................................................ ...........i....................i.........N..................................... ..................N...N......................................................... .............................N.................................................. ...........................N.................................................... .....N............N..........N.................................................. ................... Pos Score Pred ------------------------ 80 0.229 - 105 0.477 - 177 0.626 Yes 226 0.597 Yes 294 0.548 Yes 319 0.594 Yes 394 0.472 - 397 0.315 - 492 0.864 Yes 513 0.719 Yes 523 0.302 - 579 0.390 - 583 0.289 - 670 0.110 - 748 0.357 - 806 0.087 - 819 0.159 - 830 0.085 -

Explain the output -- Processing locus 928: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAAACTTATGATAAATGAAAATGGAGTATTACAATAATATTCTTCATAGATTGAAAAAATATAAAAAAAGATAAAGAT AAAGTTGAAGGGAATTGTTAAGAGTTATTCTTGAAAGTAGAAGAAGCGTGTGGATTGGACATGGGCTTAAAGACACACAA ATTCTTTCTACGAATTTTGTTAATTTTTGTTATATTTCCTTCTTCACTATTCCGCTTAGAGCACAACACCACGATGACGA CCAAGGCCATGGAGAGAGGATTTAAGCTGATTCTGGGTTCTCAATCCATGGCGAGAAAGAGGATTTTGGCTGAAATGGGA TATGATTACACTATCGTGACTGCAGACATTGATGAGAAAGCTATTAGGACAGAGAAGCCTGAAGATTTGGTTGTGGCTCT TGCTGAAGCCAAGGCAAATGAGATTATATCGAAATTGGGAGGTGAGAGCCAGTTTGCAAAAGATCCTCAACCAACTCTTT TGATTACTGCAGACACTGTTGTTGTGTACAAAGGTGTTATTAGAGAAAAACCAACCACTAAAGAAGAAGCTCGTGAATTT ATCAAAGGCTATTCCGGTTCACACGGAGGAGTTGTGGGATCTGTTCTTGTTAGAAACTTGAAAACTGGAGTTAAAAAAGG AGGATGGGACAAAGCTGAGGTTTATTTCCATGAGATACCAGAACAAGTCATTGACGGTCTTATTGATGATGCAATAACTT ATAAGGTTGCTGGAGGTTTAACACTGGAGCATCCCCTCATTTCACCCTTTATCGATTCTGTGGTGGGAGGGGTTGATACA GTCATGGGACTTCCTAAAGAACTCACAGAAAAATTCATAAACGATGTGTTGTTGTAGCTCAAGTCCCTTTTTGATTATGT AGGAATACTTTTGAATCTCTCTAATCAATCATTTATTTTGTAATTTGGTAAAACACAGATTTATCGTATCATGTTT .........N......N.....i......................................................... ............................................................N................... .........................................................................N...... ........i......................................i..........................i..... .................N.............................................................. ................................................................................ ................................................................................ ...N.........................N...................................N..N........... ................................................................................ ...N.......................................N................................N... ......................................................................N..... Pos Score Pred ------------------------ 10 0.158 - 17 0.240 - 23 0.669 Yes 141 0.486 - 234 0.390 - 249 0.500 - 288 0.790 Yes 315 0.792 Yes 322 0.228 - 352 0.105 - 418 0.083 - 644 0.342 - 670 0.138 - 706 0.096 - 709 0.222 - 804 0.181 - 844 0.418 - 877 0.082 - 951 0.066 -

Explain the output -- Processing locus 929: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACCGTTTAACCAAAGGGAAACATAAAAGAGTGGTAACCATAATCGAACATGGAAGCCACATTGTAGTTGACATATCCTAA AGGTCCAAATTCATTAATTAATTCATGAATTCATCACTTATAAATAATCACTCACCAAAGTCATTTCCCTTCACCACCCA CAAAGCCATGGCGACCACTTCATCGACAAAACAAGTCATTGTCTTCACAATTTCCCTTCTCTTGGTTGCTTTCCAAGCAG TCCATGCAGACTATTACCGTCAGAGGCCACCGGTTACCCCTACCCCTTACGTTCCTAAGCCACGGTACCCACTTCCGAGC CCGAGCCCCAAGCCTGTTTATCGACCACCTACTACCCCTAGTCTACCCGCTGGCTCAATCGCTAGACTATTCCTTGATCC ACATAACGCTCTTCGGTCCGGACTAGGTTTGCCTCCATTGATTTGGGACGGTAAACTAGCGAGCTACGCAACATGGTGGG CTAACCAGAGGCGTTACGACTGCTCCTTAACCCATTCCACCGGCCCATACGGTGAGAACTTGTTCTGGGGAAGCGGCTCG AGCTGGGCACCAGGTTTCGCGGTGCAGTCATGGATCGTTGAAGGAAGGTCATACAACCACAACACCAACTCGTGTGACGG AAGTGGAATGTGTGGTCACTACACTCAGATGGTGTGGCGGGACACCAAAAGGCTCGGATGCGCACGTGTGGTGTGTGAGA ACGGTGCCGGAGTTTTCATCACTTGTAACTACGACCCACCGGGTAACTACGTTGGCGAGAAGCCATACTAAAAAAACAAA ACACATTAAACGGGGTTTTTGTTTGGTATTTGATTTCATTTAATATCTTTTGTATCTGTTTTCGTTATATCTTCGTGAAA TTAAAATTTC ................................................N............................... ........................N....................................................... .......i........................................................................ ...N............................................................................ ................................................................................ ........................................................................i....... ................................................................................ .............................N.................................................. .......i....................i............................N...................... ................................................................................ ................................................................................ .......... Pos Score Pred ------------------------ 49 0.251 - 105 0.232 - 168 0.722 Yes 244 0.423 - 473 0.632 Yes 590 0.473 - 648 0.561 Yes 669 0.720 Yes 698 0.228 -

Explain the output -- Processing locus 930: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTATCATCTTTGACTTCTGGCTTTGAAATCTCCTTCTCGATTTTCAAGAAATCATTTCTTGATTTTGTCTGAGTGAAAA TTTCCTTCCGGATTTAGTTAGGGTTTCAATCTGATGAATCTTGGAATGAATATCGCAGGAACAATGGTGAGAGGGAAGAT CGAGATCAAGAAGATTGAGAACGTGACGAGCAGACAAGTCACGTTCTCGAAGCGAAGAAGCGGTCTATTTAAGAAGGCTC ATGAGCTTTCGGTTCTATGCGATGCTCAAGTAGCCGCCATTGTCTTTTCTCAGAGCGGAAGATTACACGAATACTCTAGC TCCCAGATGGAGAAGATTATAGATAGATATGGCAAGTTTAGTAATGCCTTCTATGTGGCAGAGAGGCCTCAAGTAGAACG ATACTTGCAGGAGCTGAAGATGGAAATTGATAGAATGGTGAAAAAGATTGATCTCCTTGAAGTTCATCACCGTAAGCTGT TGGGGCAAGGTTTGGATTCGTGTTCAGTGACAGAACTTCAAGAGATTGACACTCAAATTGAGAAAAGCCTTCGTATTGTC AGGTCAAGAAAGGCTGAGTTATATGCAGATCAACTGAAAAAGCTAAAAGAAAAGGAGAGGGAGCTCTTGAACGAGAGAAA AAGGCTGCTTGAAGAGGTAAATATGCACCACTCATCAAAGGGTAATACTGAAGGTGGTCACAGAACCAAACATTCATCAG AGGTTGAAACCGACCTATTTATCGGATTGCCCGTGACTCGGCTATAACATATTACTTTGGTTCCATGTTTGTACACTATA ATGCTTCCATACAGTTTATAGTTTTCTGAGTGCTCATGCATGACCATCTCTTCATATATATATGTG ................................................................................ .................................i...........i.................i................ ................................................................................ N...............N....N.......................................................... ......i.....................N..............N........N........................... ...................i..............i............................................. ................................................................................ ......................N......................................................... ......................i......................................................... ................................................................N............... N..................................N...N.....................N.... Pos Score Pred ------------------------ 114 0.636 Yes 126 0.756 Yes 144 0.727 Yes 241 0.204 - 257 0.266 - 262 0.166 - 327 0.582 Yes 349 0.403 - 364 0.312 - 373 0.167 - 420 0.691 Yes 435 0.704 Yes 583 0.149 - 663 0.693 Yes 785 0.130 - 801 0.072 - 836 0.057 - 840 0.037 - 862 0.036 -

Explain the output -- Processing locus 931: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAACTATTTTTAAAAAATTAACAAGTAAGTCATGAAGAACATATAGTCTTTTTATGTTTAATGCATGTATCTACTATCTC TCCCACCTGCTTTATATATGACGCCACGTCTATGACGTAGGTCAAAACAGTAACATTAGATACTATACTATCAGACACAC ATTCATATGGCCAGAGATGACCAACCACTGGAGATGCTCTAAGAAATTTATCCTCGTAGGCTTTTCTCTTTTAAAAGTCA ACATATTGGAGCCAGATGTTTATTTTATAAATACTAAGAACCACACCAAAGAAAAAACATTTCAAAGCAAAACAAAAGAG AGAGAGTGTATCCATTTTTGGAGGGGAAAACAAAGTATGCAGCAGCCCTGTGTGATAGTGGGTTCGAAGTACTGCTCACC AAACCCTGTAGGTTTGGCGATCGTCCGTAAGGTGATGAAGATAACGGACGGTAACTTCGTGATAACGAGTGCGGACGGTA AGTTATTGTTCAAGGTGAAGGATCCTTTGTTTAGTCTTCACGGCAAGAGGATCTTGCTGGATTGTTCTGGAGCTAAGGTC TTAACTTTGAGAGGGAAGATGATGACCATGCATGACAGGTGGCAAGTGTTTAGAGGAGGGAGTACGGAGGAGGGTGCTCT ACTATACACGGTGAAAAGATCGTCGATGATTCAGCTAGCCCCTAAACTTGAGGTGTTTTTGGCTAACAACGTAGAGGAGA AGATATGTGATTTCAAAGTCAAAGGGGCCTGGCTCGACGACTCTTGTGTTGTCTACGCCGGCGATTCTGACACCATTATT GCCCACATGTGCGGAAAGCAAACGATGCGAGGCTTTTTCTTCGGAAAGGACCATTTCTCCGTCACTGTTGATAAGAACGT TGATTATGCCTTCATTGCATCTCTCATTGTCATCCTTGTAGAAATAGAAAAAGCTGGTTTTATTACGAAAATGACAACAC AAATGATAATAGGTTTTTAGGTATCAATTTTTAAAGCACTATCATATCGTTGTAGTAATTTAGGTTGTCAATACAATTTG GTGTAATGCACTAGCATCGTTTGCTTTTCGTACATCTGC ...............................N.....................N......i...N............... .................N.............N................................................ ......N.........N................N.............................................. ...............N................................................................ ....................................i........................................... ..................................N............................................. ................................................................................ ..................N..N.....N...N................................................ .........................N...................................................... ....N........................................................................... ......i.................N....................................................... .....N................................................................i......... ..N............................................................................. .....N................................. Pos Score Pred ------------------------ 32 0.448 - 54 0.390 - 61 0.542 Yes 65 0.138 - 98 0.372 - 112 0.347 - 167 0.394 - 177 0.463 - 194 0.305 - 256 0.340 - 357 0.656 Yes 435 0.200 - 579 0.406 - 582 0.289 - 588 0.393 - 592 0.271 - 666 0.256 - 725 0.427 - 807 0.606 Yes 825 0.424 - 886 0.111 - 951 0.594 Yes 963 0.237 - 1046 0.067 -

Explain the output -- Processing locus 932: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGATCTTCAAGGGAAATAAAAGTTTGGTGTGCAGCTGATGATTGATTGGTTCTGTGTAATTGTTAAATTGCATGATTAC GTAACTGAGAATTCGGTTTAACCGTTTAAATTCCGGTCTACTGAAATTGCTACGGAAATAAACCGAATTAAACCGAACCG GATCATGATTCAGTCTACTTTAGTTTTTGTGCTCCGTAGATTGAGAGAAGCCAATTTTAGGGAAAAGAGAGAGATGAGGA AGTTGAATCCGCTTCTGAAACGAGGCTTGAAGGCTATTGAAAATGGTGATTTGTTGAAGATCCTCCAAAGTGAAATTCGT CACGAAATCTCCCATCCTCGTTTCCAGGGCGTTGAGACCGGTTCTCTAGGAGATTTCAAATTGGATTGGGATTCACCGGA AAGCCAAGATATTGTTCTGAAGAGACAGTTTGATTCAGGCGAGAAGGTTGTGGTCTCTGCTTTATTGCAACCAGAACCTA TAGAACTAGAAGATGATTTAGTGTTTCCTAGGGAAGCTCATGCCAAAGTTTGTATCAAAAAGCCTGGTCTCAGCTCTATC TTGCAGTTTCATTGCCGGGTTTACGAATCAGGATCTGGATCTTCTCATTTCGACATCGAGAGCGCCTACTTTATCCGCTC TTTTGTTTCTGCTCCTTCTTCCACTTATGGAGACCATTTTTTCAGCCAAGTAGATCCCAAACTACACAGTGCACTGGAGC AATACCTAATATCTAAGGGGGTCAGTGAAGGCTTAACTAATTTCCTTTTGTGTCACCTGAACAAGAAGGAGCAGGATCAA TACGTTAACTGGTTACGCAGGCTTGAATCAACTATGTCACATTCTCCAAAGCCGTGAGTGTTTACTCCATAAGCTCATTT CTTTTCAAGTTTTGTTTTTGGAACAATGGTAAAATGCCTTAGGAGTAGTATTATTAGCTAAGAGAGTCCTTGCAAT ......................................i.................................N....... ................................................................................ ....N....................................................................N...... ..........................................i..................................... ................................................................................ ................................................................................ ............N..........................N........................................ ................................................................................ ..........................N..................................................... ................................................................................ .................................N.............................................. .........................N.......N.......................................... Pos Score Pred ------------------------ 39 0.554 Yes 73 0.168 - 165 0.311 - 234 0.411 - 283 0.505 Yes 493 0.401 - 520 0.299 - 667 0.221 - 834 0.152 - 906 0.054 - 914 0.072 -

Explain the output -- Processing locus 933: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCCTGGTGGGGGTTAAAGTGATAATTTTGTTTAATATAGGACAAAATTATAAAACTAAAGAATAGTTTTGAGGCTAATTT GAATATTTCCTAAAGATATGAACTATTTTTGGCAATTTTGGAAATCACGAGATTTTTATGACCGACGAACCCATAAAAGA CCACCAGTTCGACAACGGCGGCGACGAGGCTGAGATAACAGACGCGGCGAAGAGGTCCCTTCCGATTAAAAGTCGCAAGC ATTCAACGATGGCTTCCATCACGAATTATGTCCGATACATGGCTCACAAGCTCGAGTACTCTCTCACTCTCAGCCTTAAG AAACATACTAGAGAGAAGCTCAGTGACCGGGAACTCTTTGGTGTAGTCATGAAGAATCTATTCTATGGAAGAATAAGCTA CTTACACTCGGACAAAGGAAAAGAGATGGCCCCAACCATGGGAACAAATGAAAGCACGCTACTTGTCCGTAAGCTACCAG TTGTTGATACAAGGTACATTTTCGTAGGGGATGCTGTTGTCTTGAAGGACCCGAATGAAACAAATAAGTATATAGTCAGA AGACTAGCAGCTTTAGAGGGATCTGAAATGGTATCAAGTGATGAGAAAGATGAGCCTTTTGTTCTCGAAAAGGATCAGTG CTGGGTCGTTGCTGAGAATCAAGAGATGAAATCTAAAGAAGCATATGATAGTCGAACATTTGGTCCAATTTCAATGGCGG ACATAGTGGGAAGAGCTATATATTGTCTGAGGACAGCAGTGGATCATGGTCCAGTAAGCAACAGTGAATTTGCCATGGAC GAGGATTCCCCAATCCTGGCGGTAGAACTTGACGTGGATGAATTGGCTAAAGGCCACAAGGCGTCATAATGAAGAACCTT TCTTGTACCACATTTTCATTCACTGTGTCGATGTTTCACTTATAAACTCCAATTTAGTTGTCATTGGAGGTGTTTGTGGC AAATGTTG ................................................................................ .................N.......................................N...................... ................................................................................ ........i..................i..........N......................................... ................................................i...............N............... .........................i...........i.........N................................ ..............................N.......................N......................... ...........................i............N........N.............................. .........................i..................N............................i...... .............................................N............................N..... .....................................N..............................N........... ..............................N................................................. ..N..... Pos Score Pred ------------------------ 98 0.389 - 138 0.474 - 249 0.834 Yes 268 0.703 Yes 279 0.345 - 369 0.578 Yes 385 0.370 - 426 0.849 Yes 438 0.849 Yes 448 0.212 - 511 0.212 - 535 0.092 - 588 0.783 Yes 601 0.182 - 610 0.100 - 666 0.628 Yes 685 0.124 - 714 0.818 Yes 766 0.286 - 795 0.458 - 838 0.091 - 869 0.465 - 911 0.059 - 963 0.056 -

Explain the output -- Processing locus 934: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTGGTCCTTTCTTCACCCAATTTCTCCAAGTTTCCAACTCATGAATACTTGTAGTTTTTTATAAAAAAGAGGAAACCTT ATTGGTGCTCAAGTTATATGAGTGATGATTGGATTCACTATCTTACTTTCCACTTCTATTAGTATTGAGATAAGTTTAGC TACAACTTTGTCAGTTATCGTTGAATCTAATTTGATTTGTGAATATTCTTTAGTGTTTTTTCATCACATGGCCTCCTTTG ATGGGCCAAAGTTCAAGATGACTGATGGCTCCTATGTTCAGACTAAGACCATTGATGTTGGATCATCAACAGATATCTCT CCATACCTTTCACTTATAAGAGAAGATTCTATCTTGAATGGAAACCGAGCGGTGATCTTCGATGTGTACTGGGATGTGGG GTTTCCTGAAACTGAAACCAAAACCAAAACGTCAGGGTGGAGTCTGTCATCGGTGAAGCTGAGCACTAGAAACTTATGTC TCTTCCTTCGACTTCCTAAACCTTTTCATGACAATCTCAAGGATCTGTACCGTTTCTTTGCTTCCAAGTTTGTCACTTTT GTTGGAGTGCAGATCGAGGAAGACCTGGACTTGCTTAGAGAAAACCACGGTCTCGTGATAAGAAACGCGATTAACGTTGG GAAATTGGCTGCGGAAGCTCGTGGAACTCTGGTGCTTGAGTTTCTTGGGACAAGAGAATTAGCGCACAGAGTATTGTGGT CTGATTTGGGCCAGCTGGATTCGATTGAGGCCAAATGGGAGAAGGCAGGTCCTGAAGAACAACTTGAGGCTGCAGCCATT GAAGGGTGGCTCATCGTCAATGTTTGGGATCAATTGTCCGATGAGTGATTTACTACTTATCTCTGTGATTGCTTCTTTTT TTTTTTGGTTCTTTTGATGGAAATGACTTTGTACCTGAATCAAAGTGATCCTTTGTAAGTTGTTTGA .........................................N...................................... .................N......N....................................................... ...................................................................N............ N................i......i........N....................N......................... .....................................N.......................N...........N...... ...........................................................................N.... ...........................N.................................................... ................................................................................ ................................................................................ ..................................i............................................. ...................N....................N....................................... ................N.....N............................................ Pos Score Pred ------------------------ 42 0.151 - 98 0.246 - 105 0.481 - 228 0.447 - 241 0.264 - 258 0.763 Yes 265 0.603 Yes 274 0.235 - 295 0.270 - 358 0.487 - 382 0.436 - 394 0.214 - 476 0.259 - 508 0.222 - 755 0.564 Yes 820 0.125 - 841 0.117 - 897 0.054 - 903 0.083 -

Explain the output -- Processing locus 935: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAGGATTAGACTAGTCGTCTTTATACATACAGTACATCTCATATATACCAACCAAACCTCTCTCTGCATCTTTATTAA CACAAAATTCCAAAAGATTAAATGTTGTCGAAGCTCCCTACACAGCGACACTTGCACCTCTCTCCCTCCTCTCCCTCCAT GGAAACCGTCGGGCGTCCACGTGGCAGACCTCGAGGTTCCAAAAACAAACCTAAAGCTCCAATCTTTGTCACCATTGACC CTCCTATGAGTCCTTACATCCTCGAAGTGCCATCCGGAAACGATGTCGTTGAAGCCCTAAACCGTTTCTGCCGCGGTAAA GCCATCGGCTTTTGCGTCCTCAGTGGCTCAGGCTCCGTTGCTGATGTCACTTTGCGTCAGCCTTCTCCGGCAGCTCCTGG CTCAACCATTACTTTCCACGGAAAGTTCGATCTTCTCTCTGTCTCCGCCACTTTCCTCCCTCCTCTACCTCCTACCTCCT TGTCCCCTCCCGTCTCCAATTTCTTCACCGTCTCTCTCGCCGGACCTCAGGGGAAAGTCATCGGTGGATTCGTCGCTGGT CCTCTCGTTGCCGCCGGAACTGTTTACTTCGTCGCCACTAGTTTCAAGAACCCTTCCTATCACCGGTTACCTGCTACGGA GGAAGAGCAAAGAAACTCGGCGGAAGGGGAAGAGGAGGGACAATCGCCGCCGGTCTCTGGAGGTGGTGGAGAGTCGATGT ACGTGGGTGGCTCTGATGTCATTTGGGATCCCAACGCCAAAGCTCCATCGCCGTACTGACCACAAATCCATCTCGTTCAA ACTAGGGTTTCTTCTTCTTTAGATCATCAAGAATCAACAAAAAGATTGCATTTTTAGATTCTTTGTAATATCATAATT ................................................................................ .....................i........................................................i. ................................................................................ .....i....................................i..................................... ...........................................N.................................... ................................................................................ ................................................................................ ................................................................................ ............................................................................N... ...............N................................................................ .............................................................................. Pos Score Pred ------------------------ 102 0.734 Yes 159 0.634 Yes 246 0.565 Yes 283 0.699 Yes 364 0.330 - 717 0.384 - 736 0.249 -

Explain the output -- Processing locus 936: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAGATTTTCCCCCATAATCAAGAGATGCTACATTCACCTTCCTACCCCTATCCTTTTACACGTAAAACACGTAAACATAG GAAATATCTGAGGGTAATTAAGTAATGTTCTACGGACCTACGACTTTAAATTCTTACCCACCACCACTCTCCTTTTCATT TGCACACATAACCCTAATTTGGTCTCTTACTCTAACCCGAGAGATAATGGCTCCTACACAAAATCTCTTCCTTGTCGCTA TTGCCTTCGCCGTCATTTTCACCGCCTCCACCGTACATGGTCGTCATAATGGTGCAGAAGATATCGTGCATTCCTCCTGC GAACACGCGAGCTATCCATCACTATGCGTCCGTACTCTTTCGTCATACTCCGGTCCAACCATCACCAACCGCCGCGATCT AGCTCAAGCCGCCATAAAAATCAGCCTCTCCCACGCTCAAAGCGCCGCGAAGAAACTCGCGGTGGTGCGAGACTCGGTGG GGAAGAAGAAGCAAGAGAAAGCGGCGCTTGTGGACTGTGTGGAGATGATTGGAGACTCTGTGGACGAGCTAAGCCGTACG CTCGGCGTTTTGAAGCACCTCCGTGTCTCTGGCGGTTCAGCTAAAGAGTTCCGGTGGCAGATGAGCAATGCACAGACGTG GGCTAGCGCGGCACTTACGGATGACGACACGTGTCTCGATGGGTTTCAAGGGATGGACGACGGTGAAATCAAAACGGAGG TGAAGCAGTGGATGACGAAAGTGGCCAGGGTTACGAGCAACGCGCTTTACATGGTCAACCAGCTAGATGAGACACGTGGC AAACCCCACGACGTACATCTTTGATGTTAATGCACTAGATCGTTTTAATGGACGGTCTTTATTGTGTGTGTTGGTTTTTC GTTCATGTGTTTTAAGTGTGAGATTATCTATCGCTACGATATA ........................N....................................................... ........................N....................................................... ..............................................i................................. ....................................N...........i............................... .......................N........................................................ ................................................................................ ............................................N................................... ............................................................i......N............ ....................N.................N.............i........................... ...........N......................................N...............N............. .......................N.....N.................N................................ ....N...................................... Pos Score Pred ------------------------ 25 0.493 - 105 0.421 - 207 0.834 Yes 277 0.333 - 289 0.703 Yes 344 0.322 - 525 0.320 - 621 0.634 Yes 628 0.448 - 661 0.223 - 679 0.259 - 693 0.672 Yes 732 0.473 - 771 0.134 - 787 0.118 - 824 0.152 - 830 0.109 - 848 0.153 - 885 0.039 -

Explain the output -- Processing locus 937: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCCGGTAAAAAAGCAGAGATCCCTTAACCATGATTTCCTTCAAAGTATATTCCCCTTCCTCATCCTCTTCCCATGCTCAT GGTATCAATCTTCCACTCTGACCCAACTCAAGCTCTGATTTCTCCTACTCCGTCGCCAAAAATCTTGGAAGTAGATGTCT TAGCCCGGGAAAATCAAATTTTCTCAATAATCAATCTATGGACGTTCCACCACGCCGCTCTTTGGCTGCGTCTCTCTCGC CTCTCCGGCTAATCGATGGACTCCCTCGTCGGAGGAGTTTCAATTATAATCAGATGCCTGAAGAGCCCATCAAACTCACT GTTCTCAAGCTTGATGGATCTTCCTTTGTGTTGAAGACAGCGACAGTAGGGGAGCTCAAGATGGCAGTAGAAGCTGCGTT TTCTCACTTGCCCATCTCAGGCCCTGGCAAGATCTCATGGCCGCATGTTTGGGGACAATTCTGCTTATCTTATGAAGATA AAAGATTGATTAATGAGTCTGAGTACCTCACTGAATTTGGCATTAAAGACGGCGATCAGCTTCGGTTCATCCGCCATATC TCAAACTACTGCATGTTAATGGTGAAGCATAAGAGCAAGACACCCCGTGTTTCTTCTTTCAAACAACTCAAACTGTTCTC AACCACACCGGAAACCCGGAAAAAGAACGTAATAAGGGAAGTTGAAGAAGACGGTGTTGTTGACTCCATCCCTCGGAGCC AACCAAGCTTTCTAGCAACTGTATTGGGAGGTTTGCTCTCTTACAAGTCGACGCCAAGTCAACGAGGTACTAAATACAGA AACGTGACTGCAAGTAGAGTTTTTAACAAACTCATTGCAAGGTTTCGTTTCAAGTGTAATTCCGAGAAGGATGTGTGGAA CAGAAAAAAACTCATATCCGAAACCTAAAAAAAAGCACTCCTTTTCTATCATTTGATTCTTGTAGAGATTTTGATTTCCT AATCTTGGTGTTTATATACAAACTTGGATGTTGTGTGTGTGCAAAAT .............................i..........................................N.....N. ..........................................................................i..... .....................................i.......................................... ...............N.....................................i.......................... .............N..............................................i................... ....................................N.......N..........................N........ ............N................................................................... ............N.....i............................................................. ................................................................................ ................................................................................ ......................................................................N......... ................................................................................ ...........................N................... Pos Score Pred ------------------------ 30 0.543 Yes 73 0.412 - 79 0.272 - 155 0.671 Yes 198 0.576 Yes 256 0.406 - 294 0.863 Yes 334 0.157 - 381 0.567 Yes 437 0.193 - 445 0.185 - 472 0.357 - 493 0.329 - 573 0.157 - 579 0.630 Yes 871 0.126 - 988 0.039 -

Explain the output -- Processing locus 938: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCGCGATTTTGGATGATTCTATTTCGAATCAGACTCTTTCTCAAGCTGATTCAATGTTCAGTTTCCAGATCTGGATTCT AGACTTATTTCTTCATTCTAATTTCACATGCAGATGAGAAAATTTCTGGTGAATGTTTACTCGTTGTCAATCACCAGATC ACCAGAGTGTTATATATCATCGGAATTGGAAGCAAAAGATGGTACCATCTCCGTAGCTTCTGCATTTTCCGGTCACCAAC AAGCTGTTCACGATAGCGACCATAAATTCCTAACGCAAGCTGTTGAAGAAGCTTACAAGGGAGTGGACTGTGGTGATGGT GGCCCATTTGGTGCGGTGATTGTGCATAATAACGAGGTTGTCGCTAGCTGCCACAATATGGTTTTGAAATATACTGACCC AACTGCACATGCTGAAGTCACTGCCATTAGAGAGGCATGTAAGAAACTTAACAAAATCGAGTTATCAGAATGCGAGATTT ACGCATCTTGTGAGCCATGTCCGATGTGCTTCGGAGCCATCCATCTCTCGAGACTCAAGAGGTTGGTTTATGGAGCCAAA GCCGAAGCAGCTATAGCCATCGGGTTTGATGACTTCATAGCTGATGCTTTGAGAGGCACGGGGGTTTACCAGAAATCTAG TCTGGAGATCAAGAAAGCAGACGGGAATGGCGCTGCGATTGCGGAGCAAGTTTTCCAGAACACAAAGGAGAAGTTCCGTT TATACTAAAGCACTTCTCCATTACCACATTCTTCTTCTTCTTCTCCTTCATGATGGTAATTAGAAGTAGTAGAAGAAGAA CTGCACTTGCTTTAACTTAAATAAATC .............N........................................N......................... ...........................N.....N..................i........................... ......................................i......................................... ...........................................................................N.... .........................................................i...................... ........i...........................N................................i.......... ................N......i.............................................N.......... ............................N..............N.................................... ..........................N..................................................... .................................................N..N........................... ........................... Pos Score Pred ------------------------ 14 0.215 - 55 0.235 - 108 0.289 - 114 0.267 - 133 0.683 Yes 199 0.680 Yes 316 0.286 - 378 0.686 Yes 409 0.544 Yes 437 0.423 - 470 0.523 Yes 497 0.345 - 504 0.653 Yes 550 0.400 - 589 0.257 - 604 0.435 - 667 0.343 - 770 0.057 - 773 0.074 -

Explain the output -- Processing locus 939: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAAGAATGAATTTTAATCTTGGCGTTATTGGGCCTCCGCTCCTCTGGACTCTGTCGGGCTTGATCCATTTTCGTTGCTG AGTGGATCGTAGGAACCCTAAACTTGTGAAAGAAGAATGAATTTTAATCTTTTGCATTATTGGGCCTTCGCTCATATTAT TATTCAAGGCCCAAACACTGAATCTCCTCCTCTTGTAAATCGCGAGAGAAAATCAGAAAAGAGAGAGAGAGATCTCTGGC TCTGTATCGCTCGCTGCTCTTCCTCCCACAGATCGAAAACCATGAATCCTGAGTACGACTATCTTTTCAAGCTCCTGCTT ATCGGGGATTCTGGCGTAGGCAAGTCTTGTCTTCTTTTGAGATTCTCTGATGATTCTTATGTAGAAAGTTACATTAGCAC TATTGGAGTCGATTTTAAAATTAGGACTGTGGAACAAGATGGCAAAACAATTAAGCTCCAAATTTGGGACACTGCTGGTC AAGAACGGTTCAGGACTATTACTAGCAGTTACTACCGTGGGGCACATGGAATTATTATTGTCTACGATGTCACAGATGAA GAAAGCTTCAATAATGTCAAGCAATGGTTGAGTGAAATTGATCGTTATGCTAGTGACAATGTCAACAAACTCCTTGTTGG AAACAAGTCTGATCTTACTGAAAACAGAGCCATTCCTTATGAAACTGCCAAGGCTTTTGCCGATGAAATCGGGATTCCTT TTATGGAGACTAGTGCAAAAGATGCTACAAACGTAGAACAGGCTTTCATGGCAATGTCTGCATCCATCAAAGAGAGAATG GCTAGCCAACCAGCTGGGAATAATGCAAGACCACCGACCGTGCAGATCAGAGGACAGCCTGTGGCACAGAAGAACGGCTG CTGCTCAACTTGATTGACGTGACCAGCCTAGCAATATCCTTTCCAATCTTAGAACACGTGTTCCTTCTTTTGACTAGGCT CCAATTCACTACTACTTGGTTTTTACACAACA ......i......................................................................... ....................................i........................................... ................................................................................ .........................................i...................................... .................................................N........N..................... ......................................N......................................... .............................................N....................N........N.... .............N.........i......................N...........N..................... ......................................N.......................N................. ..i..................N.........................i.....i.......................i.. ......................i......................................................... ................................................................................ ................................ Pos Score Pred ------------------------ 7 0.596 Yes 117 0.671 Yes 282 0.586 Yes 370 0.177 - 379 0.113 - 439 0.420 - 526 0.119 - 547 0.174 - 556 0.209 - 574 0.289 - 584 0.622 Yes 607 0.203 - 619 0.278 - 679 0.125 - 703 0.112 - 723 0.723 Yes 742 0.198 - 768 0.842 Yes 774 0.546 Yes 798 0.695 Yes 823 0.616 Yes

Explain the output -- Processing locus 940: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAGGCCTTAATACTATGTTTGGAGCGTGTGTTTTAATCATTTTTAACTAGTATTATAGCTTTGACTCAGATCCACTCTG TAAACCAGATCCATATGGTAAGGAAAAAATAAATTGTTAAGAAGCAAGGTCAACATGAGGAGTAGAAAGAAGACAAAAAA GAACGTGTATTGTAACCAACCCATTATGTCCATAATCTCTATAAAAGGAAACTCAAGACAAGAACACAAAAGCAAGAAGA TCAGAAACAACACAAAGATGAAAACATTCCAATTGATGATGATCTCTTTCTTGTTCGTTGCAATAACAACAACATCCGGT GTGGTTAGTGAGGGTAACGACGTAGTTTATGACGGCGAAGGAGATCCGGTTAAGCCCAACGTTCCATATTACATCAGCTT CATGACGTCGGATTACAACATGTGGATCTGCCGAATGCAATATGGATCTACTGATCCCAACTCATGCCCACAACAACCGT TAATGGTCACTCATCCAAATTTAGCAGCACCAACACCAGTTATGTTTGTATTAGCAAACAAGTCAGATGTCGTACGTGAA TCAGCCAAACTTAAGATTAAGTTCGTAGGTCCCCGTCAGTGTGGTAAATCCGGGTTTTGGAAAGTAGTTCAGAGAAACTC TTCAGAAGGTGAAGTATTCCTCAACGGATCTAAATCTATGTCACACAATGACAGCACATTCGCCATCCACAAAACTAACG AATACTACAAGTTTACTTTCGGTGACGGTGACTATCCAACGACTATCAGTATGACCAACGACTATCCGATATATAGGTTG TTATCGAAGAGGTTATCTGGAGAGATGGAGATTTATTTCTACAAGAACCTGACTACGAGTGAAGGCTAAAATGTTCTGTT TTCACTTACCTTTTTATATAATGTTTTCATTTCTAAAATCAAATAAAACTTGTTGTAATAACATGATAATCAAACAAACT AAACTCGG ...............N................................................................ ..............N.......................................N......................... .........................N...................................................... .................i.................i..i......................................... ............................N................................................... ..i......................................i........................i............. ................................................................................ .....................................i.........N................................ ..................................................i............................. ........................N.............................................N......... ....................N.........................................N................. ........ Pos Score Pred ------------------------ 16 0.468 - 95 0.478 - 135 0.305 - 186 0.095 - 258 0.637 Yes 276 0.674 Yes 279 0.707 Yes 349 0.402 - 402 0.162 - 420 0.422 - 435 0.520 Yes 442 0.406 - 464 0.553 Yes 483 0.715 Yes 522 0.678 Yes 547 0.750 Yes 678 0.653 Yes 688 0.427 - 771 0.636 Yes 825 0.382 - 871 0.237 - 901 0.084 - 943 0.066 -

Explain the output -- Processing locus 941: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGATCATGCTTGAAACTAACTAATCTTAGTTTTAAGATTTTAAAGATTAAAAAAAAAAAAAAAAAAAAGATTTTAAAGA TTGTCAATTTGAACTGCTTGAATGAAATGTCAACCAAAACTGGTTTCTAAAATTATTTTTATCGTTATTCAAAACGTAAA CGCAAACGCGAATTTTATACCTGTCAGAATTTGGTACAAAATGTTCCGCAGAATCAGCCGACGTTTCCTCTTCACCACTT TCCTCTGTTTCTCTGTTTCGCTCTCTCTACAAAATTTCTCCATGAACATACTTAACAAGCTAGGTATAGGATCAAGTAGA CAAACCAACATGGATCCTTCTCCAATCGCACAAGTAATCGACGACGAAGCTCCGGCGCCGGGAAACCAGTTTACTCAATT CGGCGCCGGTTGTTTCTGGAGCGTGGAATTAGCGTACCAGAGAGTTCCAGGAGTGACTCAAACAGAGGTTGGTTATTCTC AAGGGATCACTCACGATCCTTCTTACAAAGATGTTTGTTCAGGTACCACGAATCATGCCGAGATTGTTCGTGTTCAATAT GATCCAAAAGAGTGTAGTTACCAGTCGCTGCTTGATTTGTTCTGGTCTAAGCATGATCCCACCACTTTGAATCGGCAGGG AAATGATGTGGGAACCCAATACCGTTCAGGGATATATTTCTACAACCCGGAGCAAGAGAAACTAGCACGCGAGTCACTGG AGCGGCACCAGCAACAAGTTGACAGGAAGGTTGTGACAGAGATCTTACCGGCTAAGAAATTTTATAGAGCTGAGGAACAT CACCAGCAATACTTGTCTAAAGGAGGGAGGTTTGGTCTTAAGCAATCAACGGAAAAAGGCTGCAACGACCCAATCCGCTG CTACGGGTAAAAGCCTTGCTTCTAACCGAAAGCAGAGGATGCTTCCACTTATCTATGATAGATTATAGCAATTATCGTTG AAAAATAAAGAAGCCGAGGCTACTTATGT ......N......................................................................... .....................i....i..................................................... ........................................N....................................... .........................................i...................................... .........i...................................................................... ................................................................................ ..............................N.......................N.......................N. ....................................................i........................... ..N..i.......................................................................... ................................................................................ ................................................................................ ......................................N...............N......................... .........................N... Pos Score Pred ------------------------ 7 0.207 - 102 0.593 Yes 107 0.543 Yes 201 0.232 - 282 0.547 Yes 330 0.624 Yes 511 0.192 - 535 0.401 - 559 0.336 - 613 0.683 Yes 643 0.483 - 646 0.669 Yes 919 0.074 - 935 0.060 - 986 0.045 -

Explain the output -- Processing locus 942: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTACTAAGAGACAGGAATCTAAAGAATGCAAAGGGTAAGAGTAAAAAATTCTATGGATCAGAAAACAAATTAGTCAT GCTAACCTTCATTTTATTTAAAGTTTTTTATTTTTGTCATTAAAATCCAAAAAACAAAAGCAAATGCTACCAATACAACA GACTACAAAATGTAGGAGAAATTTGGTGAAAGCATTATTGTGGTGGGATAAGGGAGAGGAAGAGAGAGCGATGGGACACG TAACGAGGCTATTGATAAACCCTTCGTCTTCCTCGCCACTTCTCCACCTCCGTTGTTGCCACCGGACTCTTGACCCTAAC TCTGTCTTCTCTCTTCGAACCTCAATCACCAAAAGCAAGGGACGATTCTCTTGTCTTTTCTCCGGCGGCAACCAGAGAGA GGAGCAAGCTCGTAAATCATTGGAAAGCGCTCTTGGTGGGAAGAAGAATGAGTTTGAGAAGTGGGACAAAGAGATCAAGA AAAGAGAAGAATCCGGTGGCGGAAATGGTGGTAAAGGTGGAGGAGGAGGAGGTTGGTTTGGAGGTGGTGGTTGGTTTAGT GGCGACCATTTCTGGAAAGAGGCACAACAGATTGCCTTTACCCTCTTAGCAATACTTGCCGTGTATATGGTGGTTGCGAA AGGTGAAGTCATGGCTGCATTTGTGTTAAACCCGTTGTTGTATGCGTTGCGAGGAACACGGGAAGGTTTGTCTTCTCTAA GCTCCAAACTCATGGGAAGAGAAGCTTCCAAAGTGAGTGGTGACAACTCAGAGGAGATGTGGAAGAAGGAAGGTTCCACT GCTAAAGAGAGCGTTGTCCGGAAATGGGGAAGTGACTGAAACACAATTCTTAAAGCTGACTCAAGAAGAATGACTTTGGG TGTTTTTGTAACATCAAGATGAGATAATATGCAATCGACCTTTTCAGTTTTGTAATGG ............................N..........................N......................N. ...............................................................N................ .........i............................................................N......... ................................................................................ ................................................................................ ...............................................i................................ ........................N....................................................... ..................................................................N............. ..........i..............................N...................................... ...........N............................................i....................... .......................N.............................................N.......... ..................N.........N.........................N... Pos Score Pred ------------------------ 29 0.391 - 56 0.324 - 79 0.256 - 144 0.419 - 170 0.768 Yes 231 0.348 - 448 0.778 Yes 505 0.478 - 627 0.383 - 651 0.720 Yes 682 0.315 - 732 0.454 - 777 0.643 Yes 824 0.293 - 870 0.098 - 899 0.055 - 909 0.075 - 935 0.045 -

Explain the output -- Processing locus 943: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGAGACAATATAATTGGCAATGAAAAAATAAAAATTCATGTTCTCTCGATTTAGTTAATGGGCTTGTGATGTTAAAAA GCCCAATAACGGCACCAGTGAGTCTCGACGTGGCAGCTAGAGTTCCAGATCTCATCTCACATCCTCAACATGCCACGTGT TCTCTTCCATTCACAGAAGAAAGAAAAGACTTCTTCAAAATTGTTAATTGAACAAGTTTCGGCGAAAACCATTATAAGCT GTGATTGTGTGATGGCTTCTTCTACTAGATTAACTATTATACAATCAAGCTTCGTCTCCGCAAGAACAAGACTCAATTAC GTCTCCAAGACAAATCATTCTGGGTTCGCTTGTCGTTCTCTTTCAAAACCCAGAAACTTGAGTCTCTCTGTTTATTCTAT GGGTTCTTCTAGTTCTTCTCCGAAACCAGACAACGTCCAAGAGGCTGAGAAGAATGAGTTTGCTTCTCTAAGTGAAAATG AATGGAAGAAACGGCTAACACCAGAACAGTACTACATCACTAGACAGAAGGGCACAGAGAGAGCTTTCACTGGGGAGTAT TGGAACTCAAAGACCCCTGGAGTATATAATTGTGTATGTTGCGACACACCTCTATTTGATTCATCAACAAAGTTTGATAG TGGAACAGGGTGGCCATCTTACTACCAACCAATTGGAAACAATGTGAAGACAAAGCTAGACCTCTCTATCATCTTCATGC CTAGACAAGAAGTTGTCTGTGCTGTTTGTAACGCCCATCTTGGTCATGTCTTTGACGACGGTCCACGCCCAACCGGAAAA CGATATTGCCTCAACAGTGCTGCTCTGAAACTTAACGCATTGGAGAAAACAAGAGATTGAGAGTTTCTGGTACAAGGATT TGTTCATATTACATAATATACTGTATCATTATATCCACTGTATTTCCTCGTATTAAGTCGTTGTATGTAAGTCAACTAT .....................i.................N...................N..........N......... .....................................................................N.......... ................................................................................ ...........N.................................................................... ..............................................................................i. .....................................................N.......................i.. ...................................N............................................ .........................................i..................................i... .............................................N.................................. ................................................................................ ................................................................N.............. Pos Score Pred ------------------------ 22 0.696 Yes 40 0.454 - 60 0.142 - 71 0.492 - 150 0.171 - 252 0.439 - 399 0.699 Yes 454 0.366 - 478 0.611 Yes 482 0.690 Yes 596 0.223 - 682 0.521 Yes 717 0.537 Yes 766 0.239 - 945 0.063 -

Explain the output -- Processing locus 944: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACCTCGCCAACACGTAGACTCTTTTCACGCGCCAAATCAAACACCATGTCGACGAGATCAACGGATTCAAATGTTTCGT GTAATACACGCTCCAACGTAGCGCGTCGTACACTTGATGCTTAATTTATGTATTTTAAGTTACGCAGTTTCCAGATTTTC AGACCCTCTGAAAGTGGAAAGAGCATTTTTTAAAAGAGTAATAAGAAAAACAATTATCTTTGAACTCTCTCTATCTCTCT CTCTCTCTCTGCGATCTATGCCTGAAGTGGAAGTGGATAACAACAACGAGAAGCCATCTGAGATTAACAGTTTCCATCAC ATGATCATAAGTAGTAGTAAAAATGTGTTGAAAATGGAAGAAGTTGAGGTGAGCAAGAAGAGGAAATTTCAGACAGATCA ATCTGATGAGTTATCGTTATTACCTCTGTCAAAACACACTTGTTTTGCCAATGTTGCTTGTTCAGAGAATACAAATGGTA ACTCGGAGATTGATACAGAGTATTCAATGTCTTCTTATGTGAACTCAACAACTTCTATGGAGTGTAACAATGATATAGAG ATGAAAGAGGAATCATCTGGATCATGCGGTGAAGACAAGATGATCTCTTTCGAAAGCCATCTAGATTACATCTATGGGAC TCAGAACTTAGAGGATTTTTCAGAGAAAGTCATTGAAAACATTCTCTATCTCGATGAACAAGAAGAAGAAGAGGAAGATG CTAAAGGATGTAGTAGTAATGCTGCTAAGTTTGTTCTGTCCTCTGGAAGATGGACTGTTAACCAAGATGATTCTACTCTA CATGAGACAAAGAAGCCTACCATTGATCAAGAATTTGAACAATACTTCTCAACGCTAATGCTGTAACAAAATGATGTTGA GACTCAAAACATATATGAAGAGATGTATGTACATAGAACTGTACTCGTTCTGTTTTGATTCTTGTCAACCTTAAAAAAAC GTTCA ..............................................N........................N........ ....................................N..........N................................ ................................................................................ .................i.............................................................. N.....................N..........i.............................................. .....N............................................N.......................N..... ..........................i.........N...................i............N.......... i......................N...............N.................................N...... .....................................................N.......................N.. .......N..........N..............................N................N............. ..............N.......N...N..................................................... ..... Pos Score Pred ------------------------ 47 0.428 - 72 0.474 - 117 0.110 - 128 0.376 - 258 0.587 Yes 321 0.134 - 343 0.287 - 354 0.514 Yes 406 0.247 - 451 0.222 - 475 0.315 - 507 0.732 Yes 517 0.203 - 537 0.708 Yes 550 0.373 - 561 0.572 Yes 584 0.236 - 600 0.499 - 634 0.166 - 694 0.122 - 718 0.324 - 728 0.395 - 739 0.155 - 770 0.436 - 787 0.170 - 802 0.091 - 858 0.357 - 871 0.128 - 874 0.185 - 895 0.070 - 903 0.161 - 907 0.057 -

Explain the output -- Processing locus 945: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACCAACATCAAACCAAATGGACACCTTAATCCGGTACACGGCGAATTAACTGTAGAGGATAAAATCAACAATTCGACG AGTAAAACGAAAAATATCTAAAAATACAGGGCACCGAACCAAATAAAGGTGAGAATGATGAGAAGCCGTTTCTTACTCTT CATTGTTTTCTTCTCTCTATCCCTCTTCATTTCCTCTCTGATCGCCAGTGATTTAGGCTTCTGCAACGAAGAGATGGCTT TAGTCGGAGGTGTTGGCGATGTCCCTGCTAATCAGAACAGTGGTGAGGTTGAGAGCCTCGCTCGTTTCGCTGTCGATGAA CACAACAAGAAAGAGAATGCACTTCTTGAGTTTGCGAGAGTTGTGAAGGCGAAAGAACAAGTTGTAGCCGGAACACTGCA TCACCTGACTCTGGAGATTCTCGAGGCAGGACAGAAGAAGCTTTACGAAGCCAAAGTGTGGGTCAAGCCGTGGTTGAACT TTAAAGAGTTACAGGAGTTCAAGCCTGCCAGTGATGCCCCTGCTATCACTTCCTCCGATCTTGGCTGCAAGCAAGGTGAA CATGAATCTGGATGGAGGGAAGTTCCCGGAGATGATCCAGAAGTGAAGCATGTTGCTGAGCAGGCTGTCAAGACCATTCA GCAGAGGTCTAACTCCTTGTTCCCTTATGAACTTCTGGAGGTTGTGCATGCTAAAGCTGAGGTAACCGGCGAGGCTGCAA AATACAACATGCTACTGAAGTTGAAGAGGGGAGAAAAAGAGGAAAAGTTCAAGGTGGAAGTTCACAAGAACCATGAAGGT GCTCTTCATCTCAACCACGCGGAGCAACACCATGACTAGTCCCTCTCCTTTGTTTATGTACTTTTGAGTGTTTACTTACT CTGTAAGATATTTTAACTTCTGATGTAAAACTCTAAAACCTTAAATAACAAATCAGGA ..................i............................................................. ......................................................N..N...................... .........................................................................i...... ..................N........................................................N.... ................N............................................................... ................................................................................ .................................N.............................................. ..........................N....................N................................ ........i...............................................................N....... ...............................N.......................N........................ ......................N................................... Pos Score Pred ------------------------ 19 0.569 Yes 135 0.324 - 138 0.326 - 234 0.937 Yes 259 0.321 - 316 0.109 - 337 0.196 - 514 0.281 - 562 0.220 - 572 0.179 - 592 0.178 - 610 0.271 - 667 0.098 - 688 0.289 - 729 0.642 Yes 793 0.145 - 832 0.288 - 856 0.055 - 903 0.041 -

Explain the output -- Processing locus 946: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAAAATACACCCGACAAAATGATTAGTGGCCATAATGTGATTATAATGAGCATGAATGGGCAAACATGTCACATTTCTC CATTACTCAATGTCTACATAAACCCTCCAATCTCACATGTTAACTCGAAACAAAGAAGAAATAAACAGAGTAAAAAACTT AAAGCTTAAAATGGGGAGGCAGCTTTATACGACGACGGTTCTCTACTTAGTCACCCTACTCTTCATTTGCCGGACAATCT CAGCCGTCCGTTTCCCTCCGGAACAACCAACAACCGATGATCTAGATTTCATCCGTACGAGCTGCAACACAACGCTGTAT CCAGACGTATGTTACACGTCTCTAGCTGGATACGCCTCCGCCGTCCAAGATAATCCGGCAAGGCTAGCTAAACTCGCAAT CGGCGTTTCTCTCTCCCGCGCAAAATACACGGCGGCTTACCTCTCCAAACTCTCACGTCGCGCTGCCTCAGCTGCTGTCC ACGACTGCGTTTCGAACGTGGGAGATGCCGTGGATCAGATGCGTGGCTCCCTAAGGCAGCTCCGAGAGATGAACCACCGT CGTCCCGGAGATCCGGCGTTTAGGTTTCAGATGAGTAACGTGCAGACGTGGATGAGCGCGGCGTTGACGGACGAGGAGAC GTGTACGGATGGGGTCACGGAGGAGATGGAAGACGGAGAGACGAAGACGGCTATTTGCGATAGAGTCGCCGACGTCAAGA GGTTCACGAGTAATGCGCTCGCTCTCGTTAATACCTACGCCAACAATGGAGCCTAACTAACTAAGCTAGGTCTTGAAATT CCTAAAGTTATAAAAACACTTGTATATATATATGTGTTAAGTTGTGTGCTTAGTTTATAATGTGTATTCGAGGCT ...................i...............N..........N.....i...i.........i............. .........N..........................N........................................... ..........i..................................................................... ....................................N........................................... ........N....................................................................... ................................................................................ ........................i.............i.............................i........... ..............................i....................i............................ ........N................i...................................................... ............N................................N.................................. ...............................N...........................N............... Pos Score Pred ------------------------ 20 0.672 Yes 36 0.188 - 47 0.394 - 53 0.631 Yes 57 0.635 Yes 67 0.644 Yes 90 0.243 - 117 0.336 - 171 0.790 Yes 277 0.282 - 329 0.433 - 505 0.735 Yes 519 0.845 Yes 549 0.598 Yes 591 0.652 Yes 612 0.583 Yes 649 0.326 - 666 0.704 Yes 733 0.181 - 766 0.301 - 832 0.061 - 860 0.060 -

Explain the output -- Processing locus 947: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCATGCAGACAATTGTTGGCAGAGTATTTTTGTTCACCTCCAAACTCAACTCTCTAATTTTGTTTCTTTATAAAATCTGA TATTTTTCAAAAATCCTATAAATAACTCCAAGGCTTATGAATCCAAAAGAGCCAAACACATCACACAACAATATGGTAAC AATGATGAGACCTACTCTCCTTATACTTCTCTTTTCCACCTTTCTCCCACAAATCCTCACCGTTGATCCACCACTCCTAC CATCCAACGGCTCCGATTTCATCCGTTTGGCCTGCAACACCACACTCTACCCGGACCTTTGCTTCTCCACTCTCTCCTCT TTTGCCAACTCCATCCAAAATGACTCAAACCGCCTCGCGCGCGTAGCCATCTCCCTCACTCTCCACAACACTCTCCACCT TCTCTCTTACCTCCAAAACGCTTATAACCGCGACCACCCAACCCCCGTCCTTAGAGACTGCTTCGAGAATCTTAAAGATG CCGTTGACGGTATGAGAGGCTCCATGAAACAGATGAAGGAGCTTGTCTCCGCGTCTGGCTCGATCGAGTCATTTAGGTTC CAGATGAGTAATGTCAAGACTTGGCTTAGTGCAGCTCTTACTGATGAGTATACTTGTACGGACGGGTTCAAAGACGTCCA CGAAGACGACTCTATCAAAGATGACGTCTGCTCTCGAGTCGATGACGTCAAGAAGCTTACTAGCAATGCGCTGGCTTTAG TTAACCGCTATGCCGACGAGTCAATAATAAATTAATAATATCTCAGTCATCGATCCTTCGAGTCTTTTGTTTTTTTTCGA AACTATGAACTTTTATACTTAAAAAAGTATTAGTAAACTAAAAGTACTACTCGT ..N............................................................................. ....................................N...................................N....... ................................................................................ ...................i............................................................ .............................................................................N.. ...........i...........N........N............................................... ...N......N................................N.................................... ....................N....................N.......................N.............. .........N...................................................................... ....N................................................. Pos Score Pred ------------------------ 3 0.151 - 117 0.151 - 153 0.499 - 162 0.399 - 165 0.542 Yes 340 0.845 Yes 478 0.474 - 492 0.626 Yes 504 0.381 - 513 0.497 - 564 0.469 - 571 0.383 - 604 0.260 - 661 0.270 - 682 0.207 - 706 0.337 - 730 0.225 - 805 0.060 -

Explain the output -- Processing locus 948: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCTTCCCTTAATGCATGTGCTAATACCAATATTCTTGTAAGCTAGTTAAATTTTTGTTGTTGTTCTTGTATATACAATT ATACATCCTTTGTCAAGTTAAAAAAGTTCTCTGTATTTGATCAACCTTTTTGGTCATGTAGGAATCAGGTCAATGGCTTC TTTCTGTAATTCGTGGAAGAGAAAACACAATGAGGCTATAAAACTTTCAGAGGAGATTGATGGAATGATATTAGAAAGAA GTTCATTGGCGGAAACATCATCTTATGCTCGAGGTCATGCTTCATATATGCGAAGAAAGATCACCATTTTGGCAAACGGA GTTCAGACTCTAAAGAATCTTCTTGCTGAAAGTCAAGGAAAATCTATTTCAGCAAAAGAGATGAGCCGGTGCAAGGATAT GGTTGAGGATCTGAGATCAAAAGCATATCAGATGGCCTCTGCTTTGGACATGTTAAAATTTTCCAATATTGGCAGCTTGT TGGGGCAAGATGATATCATGAGCAGAGTTATTGACATGGATAACCAAGAAATTGTTGGCTTTCAACGAACTACAATGAAA GTACAAGACAAAGCACTTGAGATGTTGGAGAAAGGAGTCATGCATCTAAAACGCGAGGCTCTAGCTATGAATATGGAGCT TGGCTTGCAGACAAGGCTTATCGATCGCTTAGACCACCATGTGGATGTTAGTGCCTCTGACGTAGAGGAGTTGCAGAGGA GCATCGATACTGGTGTTTGCATGACACTGCTATTGTCAGTAGTGGTTGTGGTCTTCGGTCTTTCCGAGTTTGTCAAGTTT GGACGACGAAGGATTTTAGGTTGAGTTACTTGGAATGATTAAGTTTTACTTGGTAAATTTGCATATTGGTTTTGTTATCT ATTTGGCACAGTAATGATCATCACAAAGAGGAACTCCATAGGG ...........N...N................................................................ .......................................................N................i....... .............................N.............................N....N............... ........................N...........N..........i................................ ............................................................N.................i. ...............................i.................N.............................. .........N.......i.................N......................................i..... .....................i.................N..........................i.....i....... ......................................N.....N................................... ....................N........................................................... ..................................N............................................. .............N............................. Pos Score Pred ------------------------ 12 0.328 - 16 0.145 - 136 0.187 - 153 0.794 Yes 190 0.229 - 220 0.310 - 225 0.498 - 265 0.155 - 277 0.087 - 288 0.641 Yes 381 0.463 - 399 0.528 Yes 432 0.582 Yes 450 0.319 - 490 0.118 - 498 0.677 Yes 516 0.476 - 555 0.691 Yes 582 0.645 Yes 600 0.492 - 627 0.758 Yes 633 0.644 Yes 679 0.139 - 685 0.157 - 741 0.153 - 835 0.074 - 894 0.041 -

Explain the output -- Processing locus 949: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGATAAAGGTGCCTAAGAAATGGAAAGAGTGGTTTTGTGTTCTTACTTTACATTTTTGCACACAGAAAAATGCTAGCGG TTTCCTTTTTTATCTATATAAATGGTTTCTTTCTTTCTTCATTTTGAACACAAAAGAAGAATAACCATGGGTGAAGCCGC TAAGGATCAAACTGAAGAAGTGATTCAAGCTTGGTACTTGGATAATAAGGAAGAGGATCAGAAACTTCCTCACCACAAGG ATCCTAAGGAATTTGTATCTTTGGACAAACTTGCAGAGCTTGGAGTACTTTGCTGGAGACTTGATGCTGATAACTATGAA ACCGATGAGGAATTGAAAAGGATCCGCGAATCTCGTGGTTACTCCTACATGGACTTATGTGAGGTGTGTCCAGAGAAGCT TCCAAACTATGAAGAAAAAGTAAAGATGTTTTTTGAAGAACATTTACATATTGATGAAGAGATCCGTTACTGCCTCGCAG GAAGTGGATACTTTGATGTGAGGGATCTCAATGATATTTGGATTAGAGTATGGGTAAAGAAGGGAGGTTTGATAGTCTTC CCTGCTGGAATATATCATCGCTTCACCGTGGACTCGGACAACTATATGAAGGCAATGCGTCTTTTCGTGGGTGGACCAGT GTGGACTGCTTATAATCGCCCACATGACCATCTTCCTGCAAGGAAAGCGTATATGAAAAAATTCTTGAAGGTGATTGGGG ACCGTAACATAGACGCTTCAGCGTAAAGAACTCTCGGGGATCAGATTTCTATAAGCTAAACATATGTTTAGATGTTGTCT TTAATTATGCTTTATTATGTTTACAACATGGAAACCGAAGACATT ....................N.................................................i......... .....................i............................................i............. ................................................................................ ...............................................................N...........N.... ....N...........................................N.......N....................... ........N................N...........................N.......................... ...............N..............N..................N.............................. .............................................N........i......................... .......................N............................N........................... ...............................................................N.......N........ ......N.........N..........N................. Pos Score Pred ------------------------ 21 0.420 - 71 0.548 Yes 102 0.776 Yes 147 0.840 Yes 304 0.175 - 316 0.382 - 325 0.425 - 369 0.317 - 377 0.352 - 409 0.295 - 426 0.447 - 454 0.480 - 496 0.440 - 511 0.160 - 530 0.395 - 606 0.280 - 615 0.569 Yes 664 0.200 - 693 0.319 - 784 0.044 - 792 0.113 - 807 0.080 - 817 0.040 - 828 0.084 -

Explain the output -- Processing locus 950: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCTCGAATCAATCAGGCTTGAATAGTAGACTCGTAGTTAGTACATTGCTTTACACCTTTTGAGTTATGATTAGTGTACT TTTCCGGATTGAACCGATTGATTTGAGCCGTTAACTAAAGAACTTGTTAAGAAAAAAAAAACAGAAATCTTCAAGAATAA TATTTATATTAAAATCAGAATTGTAAAGTTTCATAAACATATGAAATTGAACAGGTTAGCAGGAGTGGCAATGGTGGCTC CGGTGGTGAATTTCCGGTGGCCGTCTATTCCAAAGAGTCTGATGCCAAAAGACTACAGAAGAGAAGTGGCGAAATGGTCT GTTTGGATAGCTAACTATTTTCCGGGACTTTTGCAATGGTTGGTGACTCAGAACATGTTCTCTACAACTTCCATGCTAGA GAAAAACCCTGTCTACTTCAACGACCAAGACATTGAAGTCCTTAAGCACATCAAAGGCTTCCCAATGCTCACTAAGGAGA AGTTACGGGAGCGCGGCGTCTTCGGGACGCTGAGAAGCGATTTTTTGGTAGCATTCGGCGACTGGGACTTCGATCCGGCA GACCTACCTGACCCGTCTCTGTCTGGACCGGAAAAGGGCTCTTCTTCTGTTCACATATGGCAAGGCTACGAAGACAAAGT GATGCCATTTCAGCTGCAAAGATGTTTGTGCCGCAAGCTACCATGGATTAGGTACCATGAGGTCCCTAAAGGAGGCCACC TGATCGTGCACTACGACGGCATATGTGATGCGATTTTGAAATCACTTCTTTTAGGAGAAGATCTCCCAATGTACAAACCA AAAGCTGTGATCACTGAGCCAGCTTAGAGACTAAGTTACTATTCTTAATTTATTCGGTTTCTTGCTTGATTCTTTGATTT TGTTTGACAAAAATCGAATTCTCTTATTTGTAAAATATATATATGT ..................................................................N............. ................................................................................ ........................................N.............................i......... .........................................N...............................i...... ...................................N..................i.................N....... ................................................................i............... ................................................................................ ........................................................i....................... ......................N....N........................................N........... ................................................................................ ..........................................N... Pos Score Pred ------------------------ 67 0.098 - 201 0.197 - 231 0.533 Yes 282 0.094 - 314 0.787 Yes 356 0.434 - 375 0.665 Yes 393 0.487 - 465 0.683 Yes 617 0.584 Yes 642 0.626 Yes 662 0.686 Yes 683 0.331 - 697 0.567 Yes 743 0.260 - 748 0.223 - 789 0.326 - 923 0.046 -

Explain the output -- Processing locus 951: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGAAAAAAATGGTCATAAGAAACAAAATCTTGGATGGTATAAAATGACTAACAGCATTGCTGTCGAAATTCTGCATCAA AAAACAAGAGAATTCATATAGTACCCAAAAATTCTCTTATTACCAAAACACTCCCATAACCACCAACAAGAAAATAAAAA GGGCATGAATTTTCCAGATTCTTCATTGTTCACACCAAATTTTGCTTATGAAAATGATTTAGATTTCTCTAGTTTGATCA CTCCTTCAACGCGTGTATCATTCCAAGAACCTAAACCATGTAATCCAGTCATTCATAGTGCAGGGATTGAGAACGATGGA AGACAAAACTGTGAGACGACCATGACATTAAGCGAAATCATGAAAGGAGACGATGAGCCGAAGAACAAGAGAGCTAAACA TAAAGAGCTTGAGAGACAAAGAAGGCAAGAAAACACATCTCTATTCAAGATTCTAAGATATTTATTGCCATCTCAATACA TAAAGGGTAAACGTTCCTCGGCAGATCACGTTCTAGAAGCAGTGAATTACATCAAAGACTTACAGAAGAAGATCAAAGAG GTCAGCGAAAAAAGAGATAGAATCAAGAGATCTATTACTCATCCATCATCAAGAGGAGAATTTTCAATAAGATCATTAGC ATCATCAACTTGTTCTTGTGTTGGAGACACGAACATTGCTGTTGTGGTTAGGCCTTGTTTGATCGGACTAGAGATCGTAG TAAGTTGTTGTAACAGACACGAATCTTGTCTATCAAGTGTTCTTCAACTCCTGGCTCAAGAGCAATGCTTTAATATTGTT AGTTGCATCTCAACTAGACTACACCAAGGATTCATACACACCATTGCTTCTGAGGTCGAGGAGGGAATAGAGGTTTATTT CTCAGAGCTTCAAGAAAAGATAATCAAAATTGGAACCTCAAGAGTCACTACTCGCTAGTCAGAGTTTATTAAACCTAAAA ATTTTATGAATTTACGTCCTAATTAAGATATATATCCTCAACCATTCCCACCACCACAAGCATGTTGTGTTTTTAGT .........N........................i.........i................................... ................................................................................ ....N..........................................N.....i.......................... .....................................i.....................................i.... .....................i.................N............N........................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ................................................................i............... ................................................................................ ................................................................................ .....N.......................................................N............... Pos Score Pred ------------------------ 10 0.426 - 35 0.512 Yes 45 0.645 Yes 165 0.466 - 208 0.107 - 214 0.611 Yes 278 0.642 Yes 316 0.552 Yes 342 0.537 Yes 360 0.212 - 373 0.344 - 785 0.520 Yes 966 0.053 - 1022 0.049 -

Explain the output -- Processing locus 952: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATGCATGTAAGCATTCGGTTAATTAATCGAGTCAAAGATATATATCAGTAAATACATATGTGTATATTTCTGGAAAAAG AATATATATATTGAGAAATAAGAAAAGATGAAAATGGAAAATGGTATGTATAAAAAGAAAGGAGTGTGCGACTCTTGTGT CTCGTCCAAAAGCAGATCCAACCACAGCCCCAAAAGAAGCATGATGGAGCCTCAGCCTCACCATCTCCTCATGGATTGGA ACAAAGCTAATGATCTTCTCACACAAGAACACGCAGCTTTTCTCAATGATCCTCACCATCTCATGTTAGATCCACCTCCC GAAACCCTAATTCACTTGGACGAAGACGAAGAGTACGATGAAGACATGGATGCGATGAAGGAGATGCAGTACATGATCGC CGTCATGCAGCCCGTAGACATCGACCCTGCCACGGTCCCTAAGCCGAACCGCCGTAACGTAAGGATAAGCGACGATCCTC AGACGGTGGTTGCTCGTCGGCGTCGGGAAAGGATCAGCGAGAAGATCCGAATTCTCAAGAGGATCGTGCCTGGTGGTGCG AAGATGGACACAGCTTCCATGCTCGACGAAGCCATACGTTACACCAAGTTCTTGAAACGGCAGGTGAGGATTCTTCAGCC TCACTCTCAGATTGGAGCTCCTATGGCTAACCCCTCTTACCTTTGTTATTACCACAACTCCCAACCCTGATGAACTACAC AGAAGCTCGCTAGCTAGACATTTGGTGTCATCCTCTCAACCTTTTTCATGTTGATATATTATATATAGATGCATAAAGAT TCGATCCAA ...........................i.....N......i....N.................................. ........................................i..i..........................i......... .........N...................................N................i................. .....................................N.......i...N....i........i........i....... ....N........................................................................... ................................................................................ ...i..............N............................................................. ......................N..............................................N.......... ...............................................N....................N........... ......... Pos Score Pred ------------------------ 2 0.313 - 6 0.350 - 59 0.214 - 108 0.610 Yes 114 0.455 - 121 0.712 Yes 126 0.323 - 201 0.537 Yes 204 0.838 Yes 231 0.665 Yes 250 0.447 - 286 0.187 - 303 0.714 Yes 358 0.361 - 366 0.921 Yes 370 0.296 - 375 0.914 Yes 384 0.647 Yes 393 0.628 Yes 405 0.454 - 564 0.562 Yes 579 0.239 - 663 0.314 - 710 0.187 - 768 0.049 - 789 0.057 -

Explain the output -- Processing locus 953: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCAGAAGGGTTTAGAATTCTTCTGGTGGAAGAGAGAAAGTAATCAGCTGAGACTTTGCGACGGAGTAGGTTTGGTCTTTC TTCGCCACGTACGAACCCTAAATCCTCTTTTACTCATTTTCCTCCATCTTCGAGCTCCCCAAAAACCTCTGCTTTTGTCG AACCTTTCAAAAAGTAATAACCTTTCTCTTCTCTGTGTTCATTTTTGATTCGTCGATTCTTCGTTATATCACCTGGAGAA AATGTTTCTGTTTCCAGATGTTTAGATTCTTGGCTTATTTTGTTTGCACTCATTTTAGGTTTAATTTTGAATTGCTTCGA GGTTTAAAGCTTATACCTTTGGACGGCGCCAAAAACATGGTCTTCCATAGGCTATCAAGATTAGGTTCTCGCATTGTTAA AGAGCTCCCAAGAGAGAGACATTTGTCAATGTGTGGAAAGAGGATTCTTCAAAGGTCTTATGGCCAGTATCTTCAATCTT CGCCAATGCTGCAAAGGCAAACTAGGAGTTTTAAGGAAGCATTATTCTCGAATAATCATAAGTTTTGTACATCTTTCAGC ACTACCTCTGAGAAAGGTGGCGAAAAAACAGAAAAGATAAACGTTACCTTTGTTGATAAAGATGGAGAGGAAATTCATAT CAAGGTTCCAGTAGGAATGAACATCCTTGAAGCTGCTCATGAAAATGATATAGAACTCGAAGGGGCATGTGAAGGTTCTC TAGCGTGTTCAACCTGCCATGTGATTGTTATGGACACCAAATACTACAACAAACTCGAAGAACCAACAGATGAAGAGAAT GATATGCTAGATCTCGCTTTCGGGTTAACAGCAACGTCGCGATTGGGATGTCAAGTCATCGCAAAACCAGAGCTAGATGG AGTACGCCTAGCCATTCCTTCAGCTACTAGAAATTTTGCGGTTGATGGGTTTGTTCCAAAACCTCACTAGTCACTTAATA GTCTCAGAGCAAATGATTTTTCGAAAAAAAACCCATTACATAAAAATTACCCTTACGGAGAGTCATACAAATGTAATTCA GACGGTGAG ................................................................................ ................................................................................ ................................................................................ ....................................N........................................... ............................N..............................N.................... .....i.......................................................................... .............................................................N.................. ................i.....................N.....N.....................N............. ..................N..........N.......................................i........N. ...i...........................................N............................N... ............................................N................................... ............N.........................................................N......... ......... Pos Score Pred ------------------------ 242 0.251 - 258 0.312 - 357 0.356 - 429 0.489 - 460 0.425 - 486 0.613 Yes 622 0.239 - 657 0.715 Yes 679 0.204 - 685 0.230 - 707 0.482 - 739 0.139 - 750 0.275 - 790 0.725 Yes 799 0.335 - 804 0.653 Yes 848 0.241 - 877 0.358 - 925 0.372 - 973 0.171 - 1031 0.068 -

Explain the output -- Processing locus 954: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 955: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 956: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAAGTTAAAAACATGATTAGAAATTTAGAATATTTTAATAAGTACAATGTGGAAATCTAGTGTAAACTTTCCTCGAAC ATTCCCCATAGATTTATATAAATAGCTAGTGAGAATTTGTTCATGCACATATTCATCACAAAGGAACTCACTGCTCTTAA ATTTGCTCTAATTGTTATTACCTTGGTAAATAATATATTCTTGGTTCGATTCGAAATTTTCAAGCAAATGGGTATCGTTG AAAACATTCGTTCCCTTAAATTTGAAGTGAAAATCGTGGAAGCTAGAAACGTAGAGCTCAAGTCATCGCCAACCACTCTC TTCGTTAGGTTCTATCTCCATGCAGGGAATAACAGTAAGATTGAACTAAACACTCCAGAGATCCGTTCGAGATCAGACTG TGAGGTCATTTCCTGGAACCAATCATTCGGTCTGGAGTGCCAAGGGAACGAAACCGCGGTCGAGGAATTGAAGCAACAAA GTGTTGTTTTCGAACTACGGAGAAGGAAAACGACGCCGTTTTTGAAGAAGTGGTCGAAATCAGAGTTGGTGGGTAGAGGA AAGATTTTTTGGAAATCGGTTATAGAGACTGATGGGATGGAAATAGAGAGATTTGTAGTGATGGATGAGACAAAAGATCA CGTTCTGGAAGATTGTGATAAGCATAAACCTCTTTTGTTGAAGATAGCTTTAAAAGTGCAAGCTTCAAAAATCGTGAACA CTAAGAGAGTGGAAGATCTTTGTGAGTGTAAAGATTGTAGAACATGTAACTGCGTAGATTATGAAGCTTTTGTTGTAGCT TGTGCGTTAGCTGGGATTTAGTTTGTAAGAATAAAATTTAAAGAAGAGATGATTCAAAGAAACCAGAATAAGTTGTTGCA GTAAAATGTAAAAACTAAGGACCAAAGTTCAAAGAAAATG ..............N.................................i............................... ..........................................N..................................... ...................................................................i............ ................................................................................ ...................N............................................................ ................................................................................ ................................................................................ ...............................N....N.......................i...N............... ................................................................................ ...........................................N................N................... ................................................N............................... .....N...............................N.. Pos Score Pred ------------------------ 15 0.441 - 49 0.515 Yes 123 0.237 - 228 0.571 Yes 340 0.471 - 592 0.112 - 597 0.306 - 621 0.539 Yes 625 0.188 - 764 0.273 - 781 0.126 - 849 0.065 - 886 0.079 - 918 0.047 -

Explain the output -- Processing locus 957: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAAAACAACTGGAAAAAAAAGAAGAAATAAAAATGCAAAATAATTCAGGATCTCCGACCAGTTTATACAGACATCAGAA TTGAAGAAATTAGGGGATTAGATGTGTCGGAAGAGTGAAGCCATGGGAAGAAGAAGAGTAGAGATCAAACGAATTGAGAA CAAAAGCAGTAGACAAGTCACTTTCTGTAAGAGACGAAATGGTCTCATGGAGAAAGCTCGTCAACTCTCAATTCTCTGTG GATCCTCCGTCGCTCTTTTCATCGTCTCTTCCACCGGCAAACTCTACAACTCCTCCTCCGGCGACAGAGTCGTTTACGTC TCTTGGAAACGCAAGAATTTTACAATCTTTCTTTCATGGCAGGATCTTGAAGACAAAACTCAGGATTATCTTTCACACAA GGAGTTACTAGAAATAGTTCAAAGAAAGATTGAAGAAGCAAAAGGGGATAATGTAAGTATAGAATCTCTAATTTCCATGG AAGAGCAGCTCAAGAGTGCTCTGTCTGTAATTAGAGCTAGGAAGACAGAGTTATTGATGGAGCTTGTGAAGAACCTTCAG GATAAGGAGAAGTTGCTGAAAGAAAAGAACAAGGTTCTAGCTAGCGAGGTGGGGAAGCTGAAGAAAATTTTGGAAACAGG GGATGAAAGAGCAGTAATGTCACCGGAAAATAGCTCTGGCCACAGCCCACCGGAGACTCTCCCGCTTCTCAAGTAACCAC CAATCATCAACGGCTGATTTTTCATCATCCTGATTCAAAAAAGGTAAAAAAAATTCATGTGTAAAAATCATAAAGAAGCT ACATGTTTTAAAATC .................................i.............................................. .....................N....................i..................................... ......................................i.......i................................. ................................................................................ ...................................N............................................ ..................................................N.........................N... ........................................................i....................... ................................................................................ ..N.............N............................................................... ........................................................N....................... ..N............ Pos Score Pred ------------------------ 34 0.697 Yes 102 0.201 - 123 0.595 Yes 199 0.607 Yes 207 0.673 Yes 356 0.325 - 451 0.319 - 477 0.383 - 537 0.678 Yes 643 0.342 - 657 0.282 - 777 0.069 - 803 0.046 -

Explain the output -- Processing locus 958: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGATGGGCCGGACTATTGTCTTAAGAAAATATAATCTCAAAACCCAAATCTGCTAACATATATCGAACAAAAAACCTCAG GAACTCTTGAAAACTTGTTAAATGTTGAGAGCTAAAACCCCTAAATCCTCAATTCAACAAAGCTTCGTTCTTTATATTCT CCGCCAATTTGGTTCTAGAAACAAAGAAATTAGTGTTGATTCGACTCGTACGTACCAATCAGGGCCTGCTTCAAGCCCAA GCTTATCGATATGGAGACGGAAGAAAGAAATGAGCAAAGAAGGTTTAATCGCAGCCAAAGAGCTCAAGCGTCTCCAAACC CAATCAGTCCGACTTGATCGATTCATTGGTTCTCATGTCTCTCGTCTCCTTAAATCCGATCTCGTTTCCGTTCTTGCTGA ATTTCAGAGACAAAACCAAGTGTTCCTCTGTATGAAGCTCTATGAAGTAGTGAGGAGAGAAATATGGTACAGACCGGACA TGTTTTTTTACAGAGACATGCTAATGATGCTTGCAAGAAACAAGAAAGTTGATGAAACCAAGAAAGTATGGGAAGATTTA AAGAAAGAAGAGGTTTTGTTTGATCAACATACATTTGGAGACCTTGTTCGAGGGTTCTTGGATAATGAACTTCCTTTAGA AGCAATGAGATTGTATGGAGAGATGAGAGAGTCTCCTGACCGGCCTTTATCTCTGCCTTTTCGAGTTATTTTGAAAGGAC TCGTTCCTTATCCTGAGTTGAGAGAGAAAGTTAAAGATGATTTCTTGGAGCTTTTCCCTGGAATGATTGTATACGATCCT CCTGAGGATATATGTGAAGATAGTGATGAAGAAGCAAGAACAGATAGCGATCTTGAATAGAGAGGTATGTCTTGAATCAA TGCTTGGTTTGGACTGTTTAGTTAGTGCTCAAAATGTCAGTGATGATATTATCCTCGAGGCTGACCCGATGTATCTTCG ..N............................................................................. .....................i.......................................................... ................................................................................ ..........N..................N.................................................. ..................................N............................................. ...............................N.........N.....................i...............N .................N.....N..N........................N...............N............ ................................................................N............... ....i.........N.......N......................................................... ....................................N.........................i................. ...........N.............i........................................N............N .................................N........N.........................N.......... Pos Score Pred ------------------------ 3 0.286 - 102 0.609 Yes 251 0.493 - 270 0.485 - 355 0.281 - 432 0.251 - 442 0.146 - 464 0.631 Yes 480 0.413 - 498 0.301 - 504 0.243 - 507 0.340 - 532 0.147 - 548 0.347 - 625 0.156 - 645 0.513 Yes 655 0.179 - 663 0.499 - 757 0.369 - 783 0.628 Yes 812 0.194 - 826 0.523 Yes 867 0.307 - 880 0.165 - 914 0.053 - 923 0.051 - 949 0.051 -

Explain the output -- Processing locus 959: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGCCCAATAATTAAATCTCAGCCGTTGAATTAATCAACGGCTCTAGATCCTTCATCTTCCTCTCCCCCAAATACCCAAAA CCCTTCCTCTGTTTTTATTAACCAGAGAAACCCCAACTTTTTCATAAACTCTCTCCAGAGAAAACTATAATGAGCTTGAG AGAAATGAGCGAAACAAAACCCAAGAGAGATTCTGAGTACGAAGGATCAAACATCAAGAGGATGAGACTCGATGATGATG ATGACGTTTTACGCTCACCGACGAGAACTCTTTCTTCTTCTTCCTCTTCTTCTCTGGCTTACTCGGTTTCAGATTCCGGA GGTTTCTGCTCCGTCGCGTTATCTGAAGAAGAAGACGATCATCTAAGCTCAAGCATCAGCTCTGGTTGTTCCAGCAGCGA AACTAACGAAATCGCTACTCGTCTTCCATTTTCAGATCTGGAGGCTCATGAAATCTCCGAAACCGAAATCTCAACGTTAC TCACCAACAATTTCAGGAAACAGGGAATTTCATCAAGCGAGAATCTGGGAGAAACAGCAGAAATGGACTCGGCGACGACG GAGATGAGAGATCAGAGAAAGACGGAGAAGAAGAAGAAGATGGAAAAATCACCGACGCAGGCAGAGCTTGATGACTTTTT CTCGGCGGCGGAGAGATACGAACAGAAACGATTCACAGAAAAGTACAACTACGACATCGTCAATGATACGCCGCTTGAAG GTCGGTACCAGTGGGTTAGTCTGAAACCTTAGAAGCCATGGAAGAACAAAAAAAAAAAGAAAAAGAAGAAGAGACAGTGC TAAAGTTACACGTCCTAGTACTGTGATTTTTGTCACAGACAGAAGAATTTT ................................................................................ .....................................................................N.......... ....i........................................................N.........N..N..N.. N............................................................................... ................................................................................ ...............................................N................................ ..............................................................i................. ...i...................................N..............................N......... ..............................................................N................. .....................................N.......................................... ................................................... Pos Score Pred ------------------------ 150 0.482 - 165 0.710 Yes 222 0.362 - 232 0.322 - 235 0.304 - 238 0.147 - 241 0.438 - 448 0.316 - 543 0.791 Yes 564 0.642 Yes 600 0.424 - 631 0.142 - 703 0.396 - 758 0.100 -

Explain the output -- Processing locus 960: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTAGGTAATCAGATCGATATCGCTATTTGTTCATACCTAGATTAGTCAGAATCTGTAATCGAAATTCGATTAGGATGG TGTAGTTCAACGAATTCATAGATACAGAATCACTTCTTCGTAGTTATGGAAAGCTCTTTGTTTAAAACCTCTTCCGATTT TTGTATCAGAAAAACTGAAATAATGGTGTATAAGCGCAAGTCAATGGAGAAGAAAAAGGAAGGCATGGGTTCTACTATTG ATCAAAACCTGAGCTTTAAGAATATTATGAAAGATGTTGCACTCTTTGGTTCTTCGCATATGACATGGAAGGATAAGAAA GCACTGGAGGATAAGAAAGTGACTGCACTTGGTGGAAAGGCACAAAAGATGCATAGGCTACCGCTGAGTGTAGCTCGAGT GCAGATGAAGAAACAGAAAGATAGAGAAGAGAAGATGTTAGAACAGAATGTGATTCTTGGCAGATTTGGTGGGGGTGGAA GTAGCAGTAGGAAACCCGCAGAGAGAAAGCGTACGCCAGAAGAGAGAGTATTGAAGTCAACTGGAGGGATTTTCAAAGGC GGTGTTCTTGATGTTAAGCATTTGCTACGTTCTGGTCCTTCAAGTAGTAGTGACAGAGATTTCAAGACGAAGAAACCCAG TAGGGTCGAGAAGAACTTGGGAGGAGGAGGTGATGGAGGAGGCATCAAGTCTAAGGGAAAAAAGAAAGGAGGCAAAAATA AGAATAAAGGAAAGAAGAAAGGAGGTGGCAAGAAGAGAGGCAAGTAATCGCGGTAGCTCTGTAACAAGTTTTGAAGCGTT TCAAGTATCATTGTATACTTCACAAGGATTACTCTTGGAATTCAACATTACAGTGAAAGAGCTATG ............................................................................N... .............................................N.................................. ......................i....................i....................i............... ..........................N......N.........................N....i............... ................................................N............................... ....N.............................i............i................................ ................................................................................ ..........N..................................................................... ................................N............................................... ................................................................................ ...............................................................N.. Pos Score Pred ------------------------ 77 0.289 - 126 0.340 - 183 0.781 Yes 204 0.572 Yes 225 0.566 Yes 267 0.424 - 274 0.212 - 300 0.437 - 305 0.818 Yes 369 0.429 - 405 0.401 - 435 0.621 Yes 448 0.505 Yes 571 0.265 - 673 0.370 - 864 0.046 -

Explain the output -- Processing locus 961: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 962: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATAAAATTTCGGAAAAACTCTCCAAACTTAAAGGTTATTGGTCTCTATGCTTGCTAAGAGTATACCTCATGTCATATTT TCTGGTTTTGTTCAGAGATAAAATATGTTTTGTGATTCTGCAGAGACTCAGCAGTGGTTTGATAAGAATGCTCCTGACTG GGGGTTTACAGAAATGATCAGCCTCACTGAACTTCATGCGGAAGAGGGACTTCTGAGGAACGGAGAACTCACCGTTGTTG CCAAGGTAGAGGTTCTTGAAGTTGTAGGAAAATTAGATGTATCAGAGGAATCTTCACCAGTAATTGAAACCATAGATGTC AATGGATTTCAAGTTCTTCCTTCACAGGTAGAATATGCGAAGTCTTTATTTGAAAGACATCTAGATATTGCATCAAAATT CCGTCCAAAGAATCCATACCTAAAGACGGCGTACATGAATGTCCTCTTGAGTTTAACCCAAACGATATGTCAATCTCCTC AAGAACTCTCCAATGATGATCTCTCTGATGCAGGCGCTGCATTGGCATATTTGAGAGAAGCAGGGTTTGAGTTGGATTGG TTGGAGAAGAAACTTAATGAAGTAAAGGAAAAGAAAAAGAAAGAAGAGGCTTGTTTGGCCGAGATACAAGACATGGACGA ACATGTTAAGCCATTGAAGAAGAAGTACTTAGACCTGGAAGCTCAGATAGATAAGAAGAAGGCAGAGTTGCTAGCTGCAA GAGCTCCTCTCTCCTTGAACGATGACAATGTTGTCTGATTATTTTTGAGTTCTTGGGCACTTGGATTTATGTTTTGCTTG TGTTTTAATGTTTTATCTCTCGTGATGTGACAATGTCTTGATTTAGGATTTTTCTAT ...............................................N.....................N.......... ........................i..........................................N............ .............i.....................N............................................ ....................................N......................................N.... ..................................i...N...........................N............. ............N..N...........N.................................................... ................N.......................................................i....... ..N............................................................................. .....................N.....N........................................N........... .......N................N.......N........................ Pos Score Pred ------------------------ 48 0.401 - 70 0.351 - 105 0.806 Yes 148 0.244 - 174 0.760 Yes 196 0.116 - 277 0.227 - 316 0.281 - 322 0.337 - 355 0.252 - 435 0.666 Yes 439 0.465 - 467 0.417 - 493 0.308 - 496 0.339 - 508 0.308 - 577 0.151 - 633 0.524 Yes 643 0.192 - 742 0.188 - 748 0.278 - 789 0.053 - 808 0.047 - 825 0.050 - 833 0.082 -

Explain the output -- Processing locus 963: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCATTGATTTTATAAATCTCTAATTGGAATTAGACTTTAGTATTTACTATTTACTTATTTAGGTGAAATCTCATTAAAT AAACAACGACTGGTCAAGTGAATGGTTGTTTGTCTTGTATGTTTTAGTAAAATGGAGATTGAGAAGGATGAGGACGACAC AACATTGGTTGATTCTGGAGGAGACTTCGACTGCAACATATGTTTGGATCAGGTTCGAGACCCGGTCGTGACTTTATGTG GCCACCTGTTTTGTTGGCCCTGCATTCACAAGTGGACTTATGCGTCCAACAATTCAAGACAACGAGTCGATCAATACGAT CATAAGAGGGAACCACCAAAATGTCCGGTATGCAAATCTGATGTCTCCGAGGCTACGCTTGTCCCGATCTACGGACGAGG ACAGAAAGCTCCCCAGTCCGGTTCAAATGTACCGAGCAGACCAACTGGTCCGGTTTATGACTTAAGAGGAGTTGGTCAAC GTTTAGGAGAAGGGGAGAGTCAACGTTACATGTATAGAATGCCTGATCCGGTGATGGGTGTGGTATGCGAAATGGTATAC CGGAGACTATTTGGAGAGTCTTCGAGCAACATGGCACCTTACCGCGATATGAATGTCCGGTCTAGGCGACGGGCAATGCA GGCTGAGGAGTCATTAAGCAGAGTCTACTTGTTTCTACTTTGCTTCATGTTTATGTGTCTATTTCTCTTCTAATTATAAT ATATATGTGTATATAATATACCAATTGGAATGAGTATGCATATATGTAATAACAATTTGTATATTTTTAAGAGTCTTGAA TCGTTGAAATTG ................................................................................ .....................N................N............i...............N............ .......................................N...................................N.... .......................................N........................................ ....................i........N..........N....................................... ..........................N.............................N....................... .............................N........i..............i..........N......i........ ..............................N.................N...N......................N.... ..............................................N.....N........................... ....N........................N.....N.......N.................................... ............ Pos Score Pred ------------------------ 102 0.394 - 119 0.106 - 132 0.666 Yes 148 0.425 - 200 0.467 - 236 0.473 - 280 0.455 - 341 0.713 Yes 350 0.337 - 361 0.449 - 427 0.488 - 457 0.245 - 510 0.377 - 519 0.566 Yes 534 0.580 Yes 545 0.390 - 552 0.508 Yes 591 0.306 - 609 0.206 - 613 0.340 - 636 0.160 - 687 0.083 - 693 0.073 - 725 0.306 - 750 0.134 - 756 0.048 - 764 0.078 -

Explain the output -- Processing locus 964: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGTCAAACTTCAGTAGTTAGCAGTTCATAGTCATCGGAGAGGTACAAAACTAACAGTAACTGTGATGATCAACTAGTTT CTAGTCATAAAAACGATTTAGAAAACGGAAACCCTAGAAATTGGGCATATGCATAGATTTCAGAGATCGGCAAAATTTCG ACTGCACAGTCCCGAAATTAAGGGTTTCTACATCACAGCGATGAACTCAACTCAACAGTTAGATTCCGACTTACGATTCT TCAGAATACTGGAGACAAATCGGTTTATCAATCTCCCTGATTCGAGATTGACACAAACCTTAGAAATTCCGGCGGCGGCT CCTCCTTCTTTGTGGCTTAATCAAAGAGTTTTAAGTCGACGGCGAGCGGAGATAGTTCTCTCAGATGGAGGTTCGTTGGT AGATCTACAATGGGGAACTATGACTCTCGACGACGGCAGCAGATCTGGTGGATTTCTTCGTCAGTCTCTTCGCGGAGGTA CCTCTCCTCCGATGAACCTCCTTGGTAGTGGTGGCTCAGTGTTAAGAAGGTACAGAGGTAGGCGGATCGTGGTTTTCGAG CTTGGTTGTGAAGATCCTATCGATCCCGGGCAGATTTACATGATTCAAGGCGTATTAGCTATGCTGCCGTCAACGACTCC TGATGGAGAATTAATGAGAAATGGGGGAGAAAAGAAAACCATTTGGATTGTAGAAGAAGAAGGAAAGCTCTCCAATAAAG CCTCGACTTCAAGAAATTTTTGTCTCAGCCCGGTTGATCCGGTTGAGTATCAGTCGGATTAGTATGTCCGTTTTAGGCTT AGGACCGAAATAGTGAAAGCAGAGGAGTCAAAACCCTTCTGAAGTTGCGCTTTTGGCGTAGATGCTATTAGGACATGTTC .................................................................N.............. ................................................N............................... ........................................N....................................... ................................................................................ ................................................................i............... .........N.........i............................................................ ...........i.................................................................... .......................................N....................N................... ..N..........N......i........................................................... ...............................................................N................ .............................................................N............N..... Pos Score Pred ------------------------ 66 0.299 - 129 0.338 - 201 0.285 - 385 0.606 Yes 410 0.475 - 420 0.586 Yes 492 0.536 Yes 600 0.321 - 621 0.488 - 643 0.210 - 654 0.285 - 661 0.677 Yes 784 0.154 - 862 0.039 - 875 0.037 -

Explain the output -- Processing locus 965: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCGATCAAAATTGTAAATCAATCATAACACATTTTAACTAGCTCTAACACAAACTCTAAAATTTTAGATTAATTCAGAC TTGGTAACAATTTTGACTTAACTACGAGCGTCATTACCATTCTATGCATCGTTTTACACGAATTACACTCACATCTCAAA GATACATGTCCACATGCTACGTACCGAGTATCGACCACACCCGATAAACAACGGGACAAGCCCTAGAATCCCGAAAAGGC AAAAAGGTCCCTTGTTTTCTCTCTTTTTTCATGTTAAACACATACTTTTTAAATTAACAAGAAAGCCCAGATATTTTTGG TGTTTTCTTACTGAATCTAGACTTGAAGAGAATTTTAGGAAAACTCCAAAAAGCTCGTACGCTAATTCTTATCTTCACTT CCATTTTTCCTGTGGTGATGGTGAGAGTTCAAGTGTTCCAACGATGGCGAAATCCCCGGTAGAGGTGAAACTGATCCCGA TCGAAGCTACTCCTGAGAATTTCGCAGACTATGGTCAAGTCATCGAAGCCTCTCGTGACGGTGCAGGTTTCGGTCCCAAC GACGCTCAACTGGATCTCTCCAGAGGAATCCCACGGTTCTATATAATGCGGATCAGAGATACACCCTTTGACTTTTCCGT ACTCACTCACCACGCGAGCGTGACACAGTGTCTAGGATCAATAGGAGGTCATGTTTGGTACCTTGGAGTGGCTAAACCGA CACTTATTGAAGATGGTGATGATGGAAAGATGGTAGATAAATTGAAATCAAGGTCTGGTCACTTGTACGCTCCTCCTGCG GTTGAAGAGATTCGCGTCTTCAGGGTCTCGGGACCAAAGTTTATTAAACTGAACCACGGTACGTGGCATGTTGGACCACT CTTCAGTGACAGCTACATGGACTTCTACAACCTTGAGCTCAGCAACACAAATGCGGTGGATCGCACGACATATGATTTCA TCAAAAATAAAGGAGTCACCATCCGAGTTGACCCCAACGATGTTGACACATCGTCCTCCTAACTCAATCTTATCATCAGT GTGTGCTTCTGTTTTCATGAATAAATTGAGCAATCGTGAATAAAATTTGTATGCAACTGTGTACAAATTGTTTTTTTTTT ................................................................................ ...........................................i.................................... .....N.......N.................................................................. ..............................N................................................. ................................................................................ .................N.........................i.................................... ..............................N................................................. .............................................i.................................. ..................................................N............................. ............N.....N..N.......i.................................................. ...................................................................N............ ................i.................................N....................N........ .......................................N........................................ ................N.................................N............................. Pos Score Pred ------------------------ 124 0.501 Yes 166 0.321 - 174 0.283 - 271 0.188 - 418 0.089 - 444 0.827 Yes 511 0.259 - 606 0.503 Yes 691 0.295 - 733 0.110 - 739 0.225 - 742 0.205 - 750 0.592 Yes 868 0.493 - 897 0.668 Yes 931 0.418 - 952 0.194 - 1000 0.488 - 1057 0.025 - 1091 0.038 -

Explain the output -- Processing locus 966: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTCTCGCTTTGGGTGCGAGAGGTCCCGAGTTCGATTCTCGGAACGCCCCTTTTTATTTTTTTAACTCCTCAACGTCAG GCCTCACGGGTATTTTCGTAAACATACAAACGATACAACCTCATCTATTTGTATATGCGCATGATAGCAAACGTACGCAA TATTCTATTCCCCAAAATACCCCTTATCCATCATTTTTCTATCCTCTCATCTTTCATCTTCCTCACACACAATCCAATCA AAACACACAGAGAGAAGAAAAACTCAGAAGAAAAGCCAAAGAGTGAAACAAAAATGGCGTCGACGACTCTCTCAATCGCA ACAACAATCCGTTCCTCATCTTATCCTACTCTCGCTTCCATCAATCACTTCCCTTCCCGAACCACCACCATCGAATTCCC CTCTCGCTTCGGTGGTGGTTCATCATCCACATTGACCCACCGTGCAACCCATCTCCGTCCAATCGCCGCCGTCGAAGCTC CGGAGAAAATCGAGAAGATCGGATCCGAAATCTCATCCCTAACCCTCGAAGAAGCTCGTATCCTCGTCGACTATCTCCAA GACAAATTCGGTGTCTCCCCACTCTCTTTAGCCCCCGCAGCAGCGGCGGTTGCTGCTCCAGCCGACGGTGGCGCGGCGGC TGTAGTGGAGGAGCAAACAGAGTTCGATGTGGTTATCAATGAAGTTCCGAGTAGTTCTCGTATTGCAGTAATTAAAGCTG TTAGGGCTTTGACTAGCTTGGCGTTGAAGGAAGCTAAGGAGCTAATCGAAGGATTACCAAAGAAGTTTAAAGAAGGTATC ACTAAAGATGAAGCTGAAGAAGCTAAGAAGACTCTTGAAGAAGCTGGTGCTAAAGTCTCCATTGCTTAAGGTTTTTATAA AAAAAAAAAAAGAAGTTGTTATCTTTTCTGGAATTTGATTGGTCTTTTGTGTTGTTTAGTATAGTTTGCGTCTGGAATTG TTGAGAAA ................................................................................ ......................................................i.....N................... ................................................................................ .....................................................i.......................... ................................................................................ ................................................................................ ................................................................................ ................................................................................ ..........................N...........N......................................... ................................................................................ .......N........................................................................ ................................................................................ ........ Pos Score Pred ------------------------ 135 0.536 Yes 141 0.310 - 294 0.890 Yes 667 0.127 - 679 0.131 - 808 0.487 -

Explain the output -- Processing locus 967: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGTGTTTTGATCACTCACAAGGTTCTTACGAGTGTTTGCCGGAAAGCCCAAATCAACGGCTCTCTTTTATGGGCTTCTC TATTGTAAAAGGCCCATATGACCCCAAAATACAAAGGAGCGGGAAGTAGACGCGCTCATTGTTCACAAATCAGGGGCATC GACGGAGACGATGATACGGCTACGGACACTGATTGAGAAAAATCGAAGCGAAAGCAGAAAAAAGATCGTCTCTTCTACTC TCGATTCGTTGCCTGAGGGATGCATTTCAAACATAATCTCTTTCACAAGTCCTGAAGACGCTTGCGTCGCTGCTGCGGTT TCGAAGATCTTCGAATCGGCGGTGAAATCAGATATCGTATGGGAGAAGTTTCTTCCGACGGATTACGAATCTCTGATTAC TCCGTCGCGAGTTTTCTCATCGAAGAAGGAACTCTATTTCTCTCTCTGCAACGATCCTCTTCTAATCGAAGATGGCAAAA TGAGCTTATGGTTAGAGAAAGCGAGTGGGAAGAGATGCATAATGTTATCTGCAACTGCGATGAATCTATCAAGCATGGCA GATATGTCTCAGCGTTTCCTATGGATTCCATGTCCAGAATCTAGGTTTGAAACAGTAGCGGCGCTTCGCGAAGCATATAG GTTTGAGTTTAATTGCAGAATGAATACTCGTGTCCTTTCTCTAAGAACTCGTTACTCGGTCTACATTGTGTTCAAGAAAG CGGATAATTGGTGTGGCTTTAAGGGTGTGTCTATAGAGGCTGTAGTTGGGATAGTGGGAGAGGAGTCTTTTAGAAGTTTC ATATGCTTTGATACGCATGGGAAGGGGCAGGCTCGAAAGAGGAAAGTGGTGGCGAAACCAGAGTTGAGAGAAGATGGGTG GATGGAGACAGAGATTGGGGAATTCTATAATGAAGGAGGATTAATGAGTAGTGATGAGGTTGAGATAAGTACTGTAGAGG GTAAATACGCCCAGCAGAAGCGTGGTTTGGTCATTCTAGGAATTGAAATCCGTCCTGCGAAAATCCTCGAGGAGTTTTCG GTTTGTCGTTTGTGAGGCCAAAACAAAGCGTTTAGTTCTAGAGATTGAAATACTCTGATAGTCTTTTAGCTCTTTCTTAT TTTGAAAGTGTAAGAGCTTCTGGTTAGACAAAGC .....................................................................N.......... .................i.............................................................. ..........N..................................................................... ...................i............................................................ ......................................N......................................... .......................................................................N.......N .......i..........................N......i.................N..............i..... ...N................N........N.................................................. ...................i............................................................ ................................................................................ ..i.............N........................................................i...... ................................................................................ ................................................................................ .................................. Pos Score Pred ------------------------ 70 0.363 - 98 0.599 Yes 171 0.500 - 260 0.514 Yes 359 0.224 - 472 0.344 - 480 0.454 - 488 0.822 Yes 515 0.480 - 522 0.531 Yes 540 0.300 - 555 0.554 Yes 564 0.211 - 581 0.360 - 590 0.320 - 660 0.651 Yes 803 0.643 Yes 817 0.285 - 874 0.590 Yes 882 0.564 Yes 910 0.292 - 924 0.655 Yes 934 0.304 -

Explain the output -- Processing locus 968: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACACCAAAGTGTGATCTAAATTGAACGGTGCAGATTAGTTTAACATTTATCCCAAATTTTAAGAACCGTACGATGGAGCA TCTTAATGACTTAAACCCTAAAAGAAGAAAAACCCCAATCGAGCTCTGTCAATGGCTCGCTTCGTCAGATGTCTGAGAAG AACACTCTTTTCTAATTCGTCTCCTTCTATTGCTCACCGGAGCCCTAGGATTGGTTTGCCCACGAATCTAAATCCTAAAA TTTTAATCAGCCGCAGATGTCTGAGCTCCGGGAGTTACGTCTCAGAAATGCAGAAATCAGCTTTCCAGGGCAACATTTTG CGACTGATCCGTAATGAGATTGAGTACGAACTCGATCACTCGCCTCCTCTTCAGCCTCCTAACAGTTTTGGTCCATTCAC TGTGGATGAACGGCCTGGAGAGCAGTGGATTAGCTTGAAAAGGAATTTTGGGGATAAAGAAGACATCAAAATTGAAGCAA CTATGTTTGATAGATCAGTTCCCACGTCAAAATCTACCAAAACTGAACCTGAATATATTCTTCACATCACTTTCATTGTC AACATCTCCAAGGCTGGTGCTACTGAAGCTTTAGAGATCATGTGTTCTGCTTGGCCAGATACCATAGAGATATCGAAGCT CTGCATTCGTAGAGGCATCAACACTTCACCCTCTTCCTATGGTGGACCAGAGTTTGAGGAATTAGATGATCAGCTTCAAG ACGCACTCTATCAATTTTTAGAGGAAAGAGGTATAAGCGACGAGCTTGCTGTTTTCTTGCATCGGTATATGAAGAACAAA GGTAAAGCTGAGTACGTTAGATGGATGGAGAGCGTTAAATCCTATGTTGAGCAAAAATAGGCATCAGCCTGCAGCAAAAG TCTTCGATCTTCTACTGTTTGGTTCCATGGAATGCTCAGTGGCCGTTGTGTATGTGAGTATGACTCTCTTTGATTTGGA ........................................................................N....... .....N.............................................N................N........... ................................................................................ ................i..............................i................................ .............N.................................................................. .....N.......................................................................... ..i............................................................................. .......................................i........................................ ......................................N..........................N.............. ....................................................................N........... ....................N...N..................N.................................... ..........................N....N...................N.......N................... Pos Score Pred ------------------------ 73 0.398 - 86 0.255 - 132 0.393 - 149 0.315 - 257 0.526 Yes 288 0.719 Yes 334 0.199 - 406 0.154 - 483 0.600 Yes 600 0.774 Yes 679 0.286 - 706 0.264 - 789 0.200 - 821 0.439 - 825 0.254 - 844 0.459 - 907 0.104 - 912 0.082 - 932 0.050 - 940 0.043 -

Explain the output -- Processing locus 969: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATCACAGAGAACACAACTTGATTCTATTTCGTAGTCAACGACATCGTTTCGGTCATCAGAGTGTATTTGACGACGTCGT TTCCATAATATTGGGCTTTAAAGTCTTATGGGCCCATGTTCACAAATTCTAAAATACAAAACTTTAGAGATAACAGCCGA CGTGGCAAAACATGAAAGACCGAGTAGACATTATAGTTTTGAGCTCCTCCCATTTCATATCCTTAAGAAAAACAAAAACA AAAATCAAATGAGCTCCTTCACCACCACCAACACTCCGCCACCGTATCTCCTCCGGAAAATTTATCATCGCCGTGTAAAC CAACCGTTCTCCGTCGTATGTTGCACCGGAGAACCCCAACAAGACATCTTCACCCGCCGGAGAACCTTGACTTCACTAAT AACATTCACGGTCATCGGCGGTGCTACGTCATCAGCATTAGCACAGGAGAAATGGGGGACAAGGTCATTCATAAAGGAGA AATATTTTATGCCGGGACTATCACCTGAGGATGCGGCGGCGCGTATAAAACAGACGGCTGAAGGATTAAGAGACATGAGG GAGATGTTGGACCACATGTCGTGGAGGTACGTTATATTCTACATAAGATTGAAACAGGCTTATCTGTCTCAGGATCTCAC CAACGCCATGAACATCTTGCCGGAGAGTCGCCGGAATGATTACGTTCAGGCGGCAAACGAGCTTGTCGAAAACATGAGTG AGTTGGATTTCTATGTACGGACGCCAAAGGTGTACGAGTCCTATCTTTACTACGAGAAGACATTGAAATCGATAGACAAT GTTGTGGAGTTTCTTGCTTAATTCAGTTGTATTGTATATATGTCTTTCTTCATTCTTCACATGAAATCTCTGGACTCGTT ACATTAGATATAAAATTAAAAGACTTAATAATGTATATTC ................................................................................ ...........................N.......N............................................ ...........N.................................................................... ........i....................................................................... .................N.............................................................. ...................................................i............................ ........i.....................N...........................................i..... ...N...........N................................................................ .......N...........................N.....................................i...... ............N.................................................................N. .......................................N....................N................... ..............................N......... Pos Score Pred ------------------------ 108 0.301 - 116 0.262 - 172 0.332 - 249 0.634 Yes 338 0.374 - 452 0.740 Yes 489 0.628 Yes 511 0.306 - 555 0.541 Yes 564 0.308 - 576 0.199 - 648 0.303 - 676 0.341 - 714 0.580 Yes 733 0.167 - 799 0.287 - 840 0.087 - 861 0.028 - 911 0.112 -

Explain the output -- Processing locus 970: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAGAACTAGAAAGCTGTGTTTTTTTTTTCTTGATCCAAAAAGCCTTAACTTTGGTTATGAAACTTACTGGAAACTGTTA CTCGTCGGTTTGTGATAATAATTTTTCTTAATGTTAATCCTGGATTTTAATGGTAGCAAGTTCTTATGCCTAATGTTATC ACTGATTCCATTTTTTTTGTTTCCTTTAAGATCTGTGAAGATGTTCTCCGCTCAGAACAAGATCAAGAAGGACAAGAATG CTGAGCCAACAGAATGCGAGGAGCAAGTTGCTCAGGCTTTGTTTGATTTGGAGAACACTAACCAGGAGTTGAAAAGCGAG TTGAAAGATCTCTACATCAACCAAGCTGTTCACATGGATATCTCTGGAAACCGCAAAGCTGTTGTGATTTACGTTCCATT CAGATTGAGGAAAGCTTTCCGCAAGATTCATCCCCGTCTCGTCAGAGAGCTTGAGAAGAAGTTCAGTGGAAAGGATGTTA TCTTTGTTACCACAAGAAGGATCATGCGTCCCCCCAAGAAGGGTGCTGCTGTTCAGAGGCCACGTAACAGAACTCTTACC TCAGTTCATGAAGCTATGCTTGAAGATGTTGCTTTCCCCGCTGAGATTGTTGGAAAGCGTACTCGCTACCGTCTTGATGG TTCCAAGATCATGAAGGTCTTTTTGGATGCCAAGGAAAAGAACAACACAGAGTACAAGCTCGAGACTATGGTCGGTGTGT ACCGTAAACTTACTGGCAAAGATGTTGTTTTTGAGTACCCAGTCGAAGCTTGAAAGAAGATGATGAAGAACCATCAGGAT AGTGAAAGAGAGCTTTTGTTTATGTTTTGTGGTATTTAGGATGAAGGAAACTCTCTTGATTCAGTTCCTTGT .........................................................N...................... ..............................N..................N...............i......N....... ........................................i....................................N.. .............i.................................................................. .................................i.............................................. ..........................................................................N..... .......................i........................................................ .......N.......i.........N..................................................N... ..........i...............N........................................i............ .....................N.....................................N..N................. .....................N..................N............................... Pos Score Pred ------------------------ 58 0.167 - 111 0.153 - 130 0.290 - 146 0.695 Yes 153 0.327 - 201 0.744 Yes 238 0.399 - 254 0.631 Yes 354 0.558 Yes 475 0.206 - 504 0.728 Yes 568 0.205 - 576 0.726 Yes 586 0.165 - 637 0.185 - 651 0.521 Yes 667 0.145 - 708 0.724 Yes 742 0.165 - 780 0.230 - 783 0.415 - 822 0.053 - 841 0.066 -

Explain the output -- Processing locus 971: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATGGGAGATAGAGAGAGGTGATATGCCCATATGGCATACCACATGTGGTGGGGGATTCGAAGATTTGATCTGGACCGTC TATCACAAGAAAGAAAGATGAAAATATTTTGTTTTTGGGTTTGGACAGATGGGAGAAAAAGAAAGTGTCCCCACAAATGT CCCCAAGAAGATGGGACACACTTTTGCCTCAAAGTTGCTGTTACTGCTACCGACAAGCTTAGCTTTTTTTCCTTTGTCCT TAATTCAGAAAACAGTTTCTTCTCTCTCTACCAGTATCTATCTTTATTCTCCTTTAACTTGTATAAAACACTCAGCTTCC TCGAAGCCTCTCATCTTCATCATCAGCAGCTTCTCTATATCTCTCCTCTCTTTCAAGGATAATAACCAAAAGCTTTTCTT TTTATCTTCTCCTGCAATTCTTGAAGAAATGGATGAGTTTGTTAACCTCAAGGAAACAGAGCTGAGGCTGGGATTACCGG GAACAGATAATGTATGTGAAGCAAAAGAGAGAGTTTCTTGCTGTAATAACAACAATAAGAGAGTACTATCAACTGATACT GAGAAGGAGATTGAATCATCATCAAGGAAAACTGAAACATCCCCTCCTCGAAAGGCTCAGATTGTTGGATGGCCACCAGT TAGATCTTACAGGAAGAACAACATTCAGAGTAAGAAGAATGAATCTGAGCATGAGGGTCAAGGAATCTATGTGAAAGTAA GTATGGATGGTGCACCATACTTGAGGAAAATAGATCTGAGTTGTTACAAAGGATACTCAGAGTTGCTTAAAGCTTTAGAA GTGATGTTCAAATTCTCTGTGGGAGAGTACTTTGAGAGAGATGGATATAAAGGTTCAGACTTTGTGCCTACTTATGAAGA CAAAGATGGTGATTGGATGCTCATTGGTGATGTTCCATGGGAGATGTTCATATGTACGTGCAAGAGACTAAGGATCATGA AAGGATCAGAAGCCAAAGGTTTAGGCTGTGGTGTATGAGATATATCTTCAAGAAATCTAACAGAGACACAAAAGAACTTG AAGAAAAATAGAGTTTCTTTTGGTTCAGAGAAATCTCTGTCTGTGCTTGGGTTGTCG .................i..............................i...........................N... ..........N..................................................................... ................................................................................ ................................................................................ ............................i...N............................................... .........i...N.................................................................. ....................................................................N........... ......................................N...........i.................N........... ..i...N......................................................................... ...i....................................N................................N...... .....i..........N............N......N......N.......N........................N... ..................................N............................................. ......................................................... Pos Score Pred ------------------------ 2 0.415 - 24 0.524 Yes 32 0.407 - 44 0.336 - 98 0.511 Yes 129 0.574 Yes 157 0.137 - 171 0.152 - 429 0.739 Yes 433 0.381 - 490 0.631 Yes 494 0.211 - 629 0.165 - 679 0.228 - 691 0.520 Yes 709 0.214 - 723 0.502 Yes 727 0.135 - 804 0.571 Yes 841 0.402 - 874 0.389 - 886 0.586 Yes 897 0.467 - 910 0.252 - 917 0.153 - 924 0.179 - 932 0.432 - 957 0.240 - 995 0.214 -

Explain the output -- Processing locus 972: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCGCGTGTGAACCTAATGGTCATACGTTTTCAAGAACACGGTAACACGGACGTGAGACAAAAGTGCGAGAGAGCCTCTG TGGTCACTTTGGTCTCTCTGACAGCTACGGGTCCACTTTCTCTTTTACCCAAATATCCCCACGCGCTTCTTTTACTTGCG CGTGAACTTTCAAAACCAACCAATGCTAAATTTCCGAAACTACCCTTCACTCTGTTTTTTTGTTTTCTTCGTTCTCTTAA GATTAGTTTTCTCATCACAAATAATAATAAACTCTTTTCTTTTGTTTCATCTCTTTCTTCTTCTCTGCGTCTTTGAGGCA AGAAAAAAAGAGAAGATGAAAGCACCCAACATGGAGACGATAACGGAGTCTCTAGAGAAATCGATGATGAATTGTTCTCT GAATGATCGGAGAAGAAGGGTCGTCGGAGATGGGTTTGGGAGATCGTCGAGCAACGAACACATGACTCCGATCTCAGATA GAACATTGGAGCTCAATTCTCACTTATCTCTTCCTTGTCACTGGGAACAGTGTCTTGATCTTAAGACAGGAGAGATTTAC TACATAAACTGGAAAAACGGAATGAGAGTGAAAGAGGATCCAAGGAAAGTGATGAATGCAGATCCTGACAGTGGAGACTC ATATGGAACCGTGTGTTCAGAAGAAGATAGCTCATATTACGACAGCGAAGAGTCTTCATCAGAGTCATCTCCATCATCAA GAGAGAATCATAAAGAAGAAGAAGAAGAAGAAGAAGAGGAGGAAGAAGAAGAAGAAGATGTGCTAGTGGTTGCTGGTTGC AAAGCTTGTTTCATGTACTTCATGGTTCCTAAGCTTGTTGAGGACTGTCCTAAATGTGCAGCACAGCTTCTTCACTTTGA TCGACCTCATTCTGCATCTTCTTGAAACCCTTGTCTTTGTTTGGATTTTCCCTTTATTCAGATGAGAATTTGTTTAGGTT TCCACTTTAATCTTATATTGTTGTTATAATTTAGTGGTTTCTCA ................i............................................................... ................................................................................ ......................N......................................................... ................................................................................ ...............N..............i................................N..N............. ..N..........................i...............................i.................. ................................................................................ .....................i.............................i...i........................ ..N............................................................................. .........................................................N...................... ............N........N...............................N.......................... .............................................................N.................. ............................................ Pos Score Pred ------------------------ 17 0.564 Yes 183 0.295 - 336 0.255 - 351 0.614 Yes 384 0.477 - 387 0.321 - 403 0.331 - 430 0.575 Yes 462 0.568 Yes 582 0.773 Yes 612 0.560 Yes 616 0.630 Yes 643 0.320 - 778 0.180 - 813 0.155 - 822 0.207 - 854 0.149 - 942 0.086 -

Explain the output -- Processing locus 973: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCAAGCAAATCAGTTTATAGAGATAATAATAACTGCTACAACATATTCGTAGTATATACAAATTCTATCTTAATCCTTAT TTTTAATTTTGTTTACTTTAAAAGAAAAGAATAAAACTCTATATAAACGGATCAATGCATTCTCACTCATTTTTGTTTAT ACACTTAAAAAAAATCCTTTGTTTACATCGCCGTAACACCATGAAGAGCGTCATTGTCATTGTAGCTCTCCTATGCCTCG TCTCTCTCCCAAACCCTACCGTCGGATCAACAAAGAAACCATGGCCTAAGCCGTCCGATCTAGCCAATCACAATAACAAC TTCGGTGACTCTAAGGTTGGTTGGGCTTGCTCCTCCTCTTCAGACCCCAACACTCCTCCTTCACCTCCCGGCTCGTTCCC TAATATCCCCCAAATCCCGGGAATACCAAACATCCCGTTTCCCAACATTCCGGGAATCCCAATACCTAACATCCCTGGAC TCCCTAACATCCCTGGACTTCCAGGTCCTCCGTTTGAGTCTCTTCTCGTGTCGCAATCCGGTGAGTTAGAGAAATGTTTG TCTAAAGATGGGTCGAAAACTAATGAGAAATGTTTCTCGCAAATATTTTCGAGTTGGGCGGAGAATGATTTCGCATTGGA CAAAGAATGTTGTGAGATCATTGTGAATATGAACAAGAGGTGTTATGGCCATCTTCACATGATGTTTAAGAGTCATTTCT TTGCTCCTCTCCTTCAATACTCTTGTCACATCAAGCATGCCAAGAACTAAGCAAGTCTTCCACACGCAATTCGTTCCTCC ATTTAGTTTTCTTTCCTTATTTTTAGTTTTGTTAGGGTTTTGATTTTGTTTTGTCCATTTCTCATGGAC ................................................................................ ......................................................N......................... ........................................i...............................N....... ........................................i....................................... ................................................................................ ................................................................................ .........................................................................i...... .......N..............N......i..................................N............... ......N.....................i...............N.............i..N.................. ....................................N........................................... ...............................................................N..... Pos Score Pred ------------------------ 135 0.392 - 201 0.668 Yes 233 0.480 - 281 0.603 Yes 554 0.536 Yes 568 0.429 - 583 0.307 - 590 0.616 Yes 625 0.236 - 647 0.211 - 669 0.598 Yes 685 0.395 - 699 0.536 Yes 702 0.334 - 757 0.145 - 864 0.046 -

Explain the output -- Processing locus 974: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTTTTTTCCTCTCCATCATCTTCTCTTATCAACTTTAGACTCACAAGCCTCCTCTCATCTTCACCATAACCGAAAAACC AAACAAAAAATCCTTAGATAATCAGATCTTGATATTATGGATCATATCATCGGCTTTATGGGCACAACAAACATGTCACA TAACACAAACCTTATGATCGCTGCCGCAGCCACTACCACTACGACCTCTTCGTCTTCCTCTTCGTCCTCCGGAGGCTCGG GGACTAACCAGCTAAGCAGGTACGAGAATCAGAAGAGAAGAGATTGGAACACTTTCGGACAGTATCTACGCAATCACCGT CCACCACTTTCTCTCTCCCGTTGCAGTGGTGCTCATGTTCTTGAATTCCTCAGGTACCTCGACCAATTCGGCAAGACTAA GGTTCACACACACCTATGTCCGTTCTTCGGACACCCAAACCCACCAGCACCATGTGCCTGTCCACTCCGACAAGCGTGGG GTAGTCTGGACGCACTCATTGGCCGTCTTAGAGCTGCTTTTGAAGAGAACGGTGGTTCACCAGAGACGAACCCTTTTGGT GCACGAGCCGTTCGACTCTACCTAAGGGAAGTACGTGACTCGCAGGCTAAAGCACGTGGGATCAGCTATGAAAAAAAGAA GCGCAAGCGACCTCCTCCGCCACTACCACCGGCTCAGCCGGCGATTTCAAGTAGCCCTAATTAACATTAAGTCATGAGTA AGATGTTTCAATGAACTACGTTTGTTTACAAATTTTTATCAATGACGAACATGCACGAGTTCTTTTAAAGTCACATGCCC TTT ................................................................................ ....................................i....................i..............i....... .............i.................................................................. ................................................................................ ..................................N............................................. ...............N...................................N............................ ................................................................................ ...................................................................N............ .........................................................................N...... ..N.......N..............................N........N.......................N..... ... Pos Score Pred ------------------------ 117 0.787 Yes 138 0.572 Yes 153 0.812 Yes 174 0.526 Yes 355 0.319 - 416 0.499 - 452 0.332 - 628 0.325 - 714 0.122 - 723 0.095 - 731 0.121 - 762 0.111 - 771 0.055 - 795 0.040 -

Explain the output -- Processing locus 975: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGATTGGATGATAGTGTTAAAAGAAAAATATGAACTGAGGCAAAAAGAGGAGTGGTCCCCGGAAGATTGTGAAATGTGTC ATCTAAACCAGCCAGACGTAGTCACGTGTTCTCTCTAGCTTTATGAACTTCCTTAGCCAGCACCATCATTGTGATTGTAG TATATATGTAACCCTACCTTCATCTCTCCCATTTTCCATTCTCCATATAGACTCCTTTACAATATACAAAACCTATCCAA AAGCGAAGAAGCCAAGCAAACATATTATAAAAATGAAGAAGGCGTCAGATTTCGTGAAGCAGATAATAAACATGTTGACT TGTCTAGCAAAGGACAAGACGAAGGAGCTTAAGAGGAAGACAAGAGCCTTCAAGACACGTCTCGTAATATTTTCGCTGCT CCACAACAGACACATGGTGGTGGGTTCTTTGTCTCACAAGCTCAAGAGCAATCAAACATCAAAAAAGATAGATCATGATA ACAAGGATGTTCAAGATTATCATGATAAAAACATGGTGGTTGTGTATAGCCAAAACGTTAACGCCATGTCTAGCACTCCT GTCTCTCCGCATGTACAATATCAATATACAGAGGAGGAAGATGATGAAGAAGGGGAAGAAGAAAAGTATCCAGACTTGAG ACACTCGTTGTTTGAGGCAGAAGGTTCGGTGATAGAGATGGTGAAGCACTCCAAGGAGGACAATGGAGAAGAGTTTCGGT TGGAAGACGAGATCGACAAAGTGGCTGATCTTTTTATTACCCGCTTCCATAGACAGATGTGGTTACAGAAGCAACTGTCT CTCGAGAGTCCAGATACACCCTCCCCAATTCATTCTTAATATACATGTATTTGACGCTCCTCCTTGTTTTTTGTTTCGGG GTGAAGGACTCAATGATCCATACAGATTTTTGTTTTACGTAGTGAAAAATATGAAAAC .......N.....................i...........................................N...... ..........................................N..................................... .....N.......................................................................... ................................i......................................i........ ................................................................................ .............i............................................................N..... ......N..............N..........i................................i.............. ..........N.............................N..N.................................... .....................................N........................N................. ........................................................N....................... ............................................N................................... ............N.....................................N....... Pos Score Pred ------------------------ 8 0.334 - 30 0.734 Yes 74 0.326 - 123 0.125 - 166 0.284 - 273 0.750 Yes 312 0.526 Yes 414 0.618 Yes 475 0.156 - 487 0.207 - 502 0.246 - 513 0.867 Yes 546 0.568 Yes 571 0.300 - 601 0.421 - 604 0.241 - 678 0.469 - 703 0.317 - 777 0.227 - 845 0.092 - 893 0.056 - 931 0.050 -

Explain the output -- Processing locus 976: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACTTCACAAAGCTATGGACCGACATGACCCAACGGGTCCAAATTTTTAAAATTTGCAATCCAACTAAACTACACAAAAA TAAAAATTAAATTCTCAGTAATATATGTACCTAACTAAGGGTAAAGTGGGAAATACAAAAAAACATTTCGAAGAGAAGGA AGAAGACAAAAGAAGAAGCACGAGAATCATCTGCTTAAAATCCAATCGCATCGACTCAGTCTTCTTCTTCTTCTTCACCT ACCTTTCTCTCTCTCTCTCGTGGTCCATTCGCTATTCTATACTTCTTGTCGGCGCCGGAGAGAGTGAGATGACTTGGTCG GTTTTCAGATCCATAAATACTCCAACACTCGACCTCTCCACCGCACTTCGCTCCACTCGTACCCCATTGGTCGCTGCTGG TGTCGGCTGCGCAACATTCGCTGGTGTTTCTCTCTTCAGAATGTCTTCTAGATCTCCTCCTTTCGCTTCCCTCTCTGTCT CTGCTTCTTCTGTGAAGAAGGAAGTTGTGTCTACTGAGAAAGCACCAGCTGCTTTGGGACCTTACTCTCAGGCCATTAAA GCCAATAATCTGGTTTTTCTTTCAGGTGTTCTTGGACTTATACCTGAGACTGGAAAGTTTGTTTCGGAGAGCGTCGAAGA TCAGACTGAGCAGGTACTCAAAAACATGGGGGAGATATTGAAAGCTAGTGGTGCTGATTATTCCTCGGTGGTGAAGACAA CAATCATGTTGGCTGATTTGGCTGACTTCAAGACAGTGAACGAGATATATGCCAAATACTTCCCAGCTCCTTCTCCAGCA CGATCGACGTATCAAGTTGCAGCTTTGCCTCTAAACGCCAAGATCGAGATTGAATGTATTGCAACACTCTAGAACACTCA AATCAATCTCTACGAAGGGTATTTTGGTCCGGAAGATGTCAAAACAAATAAGAGAAACAATGGTTTTTGGGGCTTAACCC ATATTTAACCT ..............N.........N....................................................... ........................N....................................................... ................................................................................ ....................................................................i........... ................................................................................ ........................................i....................................... ................................................................................ ................................................................................ .........................i...................................................... .....i..........................................N............................... .....................................................N.......................... ...................................N.......................N.................... ........... Pos Score Pred ------------------------ 15 0.271 - 25 0.320 - 105 0.433 - 309 0.814 Yes 441 0.853 Yes 666 0.799 Yes 726 0.726 Yes 769 0.165 - 854 0.220 - 916 0.047 - 940 0.065 -

Explain the output -- Processing locus 977: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACGTTGAACTATGCATCATAAATTAAATTTTGATAATTCACATTTCTCATAATACAGAATTCTCAAACTACTAAATTAC TTTGAAATTCAGCATATTTAATTAAGAAAACATGAGTTAACAAAAATGTTTAAATTAGTGAAAAAAATCCTCATGAAGCT AGCTCTTTTTCATGTCAAATCACACCTTTTGAGTAATAAACCCAATTTTCTTGGTTCCTTCCTCACAAATTCACTTTCCA CAATTAATCTATCTCTCTCTTTATCTCCCTCACACCTCAGCCTTCTGTGTTCGTCTTTATCTCTCTCCCAAATCTCAATT TTGGACTCGTCTGCTTTTGTTTATATAAACACTTCTCTTCTTCTTCAATCTCCACACACACCACACAAAACCACCTACCT TCTTCGATTTCTCCAGAAATCCTCCTTCAAGTTCATACTCATGGAAGAAGCTAAAGTGGAAGCAAAAGATGGTACCATCT CCGTAGCTTCTGCATTTTCCGGTCACCAACAAGCTGTTCACGATAGCGACCATAAATTCCTAACGCAAGCTGTTGAAGAA GCTTACAAGGGAGTGGACTGTGGTGATGGTGGCCCATTTGGTGCGGTGATTGTGCATAATAACGAGGTTGTCGCTAGCTG CCACAATATGGTTTTGAAATATACTGACCCAACTGCACATGCTGAAGTCACTGCCATTAGAGAGGCATGTAAGAAACTTA ACAAAATCGAGTTATCAGAATGCGAGATTTACGCATCTTGTGAGCCATGTCCGATGTGCTTCGGAGCCATCCATCTCTCG AGACTCAAGAGGTTGGTTTATGGAGCCAAAGCCGAAGCAGCTATAGCCATCGGGTTTGATGACTTCATAGCTGATGCTTT GAGAGGCACGGGGGTTTACCAGAAATCTAGTCTGGAGATCAAGAAAGCAGACGGGAATGGCGCTGCGATTGCGGAGCAAG TTTTCCAGAACACAAAGGAGAAGTTCCGTTTATACTAAAGCACTTCTCCATTACCACATTCTTCTTCTTCTTCTCCTTCA TGATGGTAATTAGAAGTAGTAGAAGAAGAACTGCACTTGCTTTAACTTAAATAAATC ...........i.................................................................... ...............................N.............i..........................N....... ...........N.................................................................... ................................................................................ ................................................................................ ........................................i...........................i........... ................................................................................ .........................N...................................................... .......i..............................i...........................N............. ...................i..........................N......i.......................... ...................N......................................N..............N...... ........................................................N....................... ...............................................................................N ..N...................................................... Pos Score Pred ------------------------ 12 0.509 Yes 112 0.272 - 126 0.532 Yes 153 0.220 - 172 0.336 - 441 0.698 Yes 469 0.622 Yes 586 0.286 - 648 0.686 Yes 679 0.544 Yes 707 0.423 - 740 0.523 Yes 767 0.345 - 774 0.653 Yes 820 0.400 - 859 0.257 - 874 0.435 - 937 0.343 - 1040 0.057 - 1043 0.074 -

Explain the output -- Processing locus 978: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCGTTCCAAAATCAGCTCTTTTTGTTAATCAGGTGAGTTTTTAGGTATTTGAATCTCCAATTGCTTCCTTGCAATGAC TAAGTATTGTGAAATGTTTAGGGTTTCATCTGTGTGGGTCTTGTTTTGAAGCAATTTGTGTGTGTTTGGATGAAAGTAGC AGATATGCAGGACCAGCTGGTGTGTCATGGTTGTAGGAATTTATTGATGTATCCTAGAGGAGCATCTAATGTGCGTTGTG CGTTATGTAACACTATCAACATGGTTCCTCCTCCTCCTCCACCTCACGACATGGCACACATTATATGTGGTGGTTGTAGA ACAATGCTTATGTATACGCGTGGGGCTAGTAGCGTAAGATGCTCTTGCTGTCAAACTACGAACCTTGTGCCAGCGCACTC CAATCAGGTTGCCCATGCTCCTTCCAGTCAGGTTGCGCAGATCAATTGTGGGCATTGTCGGACGACCCTCATGTATCCTT ACGGTGCATCATCCGTCAAATGCGCTGTTTGTCAATTCGTAACTAACGTTAATATGAGCAATGGAAGGGTACCTCTCCCA ACTAACCGGCCAAATGGAACAGCTTGTCCCCCCTCTACATCAACTTCAACACCACCCTCTCAGACCCAAACCGTTGTTGT AGAAAACCCCATGTCCGTTGATGAAAGCGGAAAGTTGGTGAGCAATGTTGTTGTTGGAGTGACAACTGACAAAAAGTAAT CAAGAATGAGTGAGATCTTAAAGATCAAATCCAAATTCTTCCTCTATTCCTGCGTTTGGTTTGTGCATATTACATACGCG GAAAAACTGTATGTTATA ...........................................................................N.... .............N.......................................................N.......... ....N.....................N...................N.....................N........... ....N...............i.............................i.............i............... ...i.....i............................i......................................... ..............N.......................................................i......... ...................N.................................i......i................... .............i.................................................................. ..........i.........N.......................N................................... .....N.......................................................................... ..........N....... Pos Score Pred ------------------------ 76 0.480 - 94 0.261 - 150 0.334 - 165 0.396 - 187 0.270 - 207 0.372 - 229 0.290 - 245 0.263 - 261 0.766 Yes 291 0.550 Yes 305 0.596 Yes 324 0.871 Yes 330 0.675 Yes 359 0.512 Yes 415 0.126 - 471 0.616 Yes 500 0.304 - 534 0.530 Yes 541 0.583 Yes 574 0.621 Yes 651 0.677 Yes 661 0.181 - 685 0.229 - 726 0.184 - 811 0.044 -

Explain the output -- Processing locus 979: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 980: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 981: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 982: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 983: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTATCTAATTTTAATAACAATAATTTTAATAACATGTTTAGATGGGTGAGCCACACAAAATCACTTTTTATTTTTAATT CAAAAATAGGAAAGCATGTGAATCATGTCTATAAATAAAGGTTTCCAAAGCCTCATATTCACTCACAAAATCACTACTAC TTACTCATTTCTACTTAACATTCTAACGATGGCAAACCAAATCTACTTATTCTCCTTGATCTGCTTGTCTGTTTTACTTT GCCAATCATATACGGTCTCATCTTTCCAAAAATCACTAGACCTAGCGAAACCATGCAAGCGTTTCGTCCTCCACCTCCAT GACATTGCGTACGATGGTGATAACGCGGCTAATGCAACGTCAGCAGCAATTGTGAATCCTTTAGGACTAGGAGACTTCAG TTTTGGGAAGTTTGTGATTATGGACGACCCTGTGACAATGGACCAGAACTATCTCTCGAAACCCGTGGCTCGTGTTCAAG GCTTCTTCTGCTACCACGGTAAGGCAACATACGACGCTTGGATCGCTTGGACAGTGGTATTCAACTCAACACAACACAAA GGGGCATTCACCATAATGGGTGAAAATCCGTTTATGGAGCCGACTAGAGACCTACCGATCGTTGGTGGGACTGGTGACTT CATCATGACTCGAGGGATCGCTACGTTAACGACCGATCACATTGACGGCAGTAAGTATTTCCGCGTCAAGCTTGATATTA AGCTCTACGAATGTTACCATTAGAGTTACTAATACGTTATACGTGCATGTCTTGGGTTTGATGTTTCTGAATTGATATTT TGGGTTTGGTTCTTCTTTCTTAGCATTTTATTGCTTTTATCT ..................................N.......i..................................... ...............i........N....................................................... ............................i................................................... ....................................................N.........................N. .............i.................N................................................ ...................i.................i.......................................... ................................................................................ ...............i.................i.............................................. ....i........................................................................... ..........N...................................N.............N................... .......................................... Pos Score Pred ------------------------ 35 0.405 - 43 0.676 Yes 96 0.587 Yes 105 0.468 - 189 0.535 Yes 293 0.456 - 319 0.116 - 334 0.509 Yes 352 0.110 - 420 0.725 Yes 438 0.557 Yes 576 0.744 Yes 594 0.659 Yes 645 0.564 Yes 731 0.236 - 767 0.148 - 781 0.040 -

Explain the output -- Processing locus 984: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGCGTCGCCTTTCTTTATCCAAAAATAATCCGACTTTTAATTTAAACCAAATAATTAGCAAAATTAGTAATCACCTTGAT TAAATTAACCATCTAATATAAATAGATGAGGGGCTAAATGATATGATCTTTTTTGTTTTCTCTCACACTTTCTTAAGATT TTTTTCTTCGGCTCGGACGCAGATGACTAACAGTTACGCTCTGTCACCGGCGATTATCGCCGCCGTTCGTCCTCCGGCAT CTCAAGACTATCTAGTTGCTGCTTCTCTTAGTGGACGCAAATCGATCAACTCTTCATCTTCGTCCATTTTCGTACCTATC TCATTATCTACTTCCTACGGTCGAAGCAAATGCGCCTTCTCAATCTCGCGGAAGAATCCAAAATCGACGATTCGTTGCGA TATTGCTGTGAAATCGGCGGCTTCTGTAGACGCGGACGCTGATCTATCGTCATCTACGTCGTTGGAGACGGAGGAAGACG AGAAAGCGAAGGAGAAGATTGGAGCTAGGGTTAGGGTTACGGTTCCGTTGAAAGTTTACCATGTGGTTCGTGTACCGGAG GTTGAATTGATGGGAATGGAAGGTTTTATCAAAGATTACGTCGTTCTTTGGAAAGGAAAGAAAATCTCAGCTAATCTGCC GTTTAAGGTACAGTTCGTGAAAGAGATCGAAGGTCGTGGTCCTGTCAAATTCTTCACGCATCTTAAGGAAGACGAGTTCG AGTTAATTGATCCGTGATTGAATTGAAAAAGGCAAAAACTTTTCTTCTCTTTCTGATATTAGACTTGAAAAATCTTTTGT GAATGGTTTGTTTGTATGTACTTTTGTATACATTTA ................................................................................ .........................N...........N....N..................................... ......................i......................................................... ................................................................................ .............................N.................................................. ................................................................................ ............................................................N................... .........i.....i................................................................ ................................................................................ ................................................................................ ..N............N.................... Pos Score Pred ------------------------ 106 0.440 - 118 0.092 - 123 0.493 - 183 0.771 Yes 350 0.367 - 541 0.134 - 570 0.504 Yes 576 0.563 Yes 803 0.036 - 816 0.033 -

Explain the output -- Processing locus 985: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTACGTCCACATCTCAAAAGGCTCAATCTTTGGAGTTTGAACGGTTGTTGTAAATAATCAAATTTTGTACTAGAGCCTA ATTGTGATTAGTCGATGATAAAAGAATATAGAATCCAATATAATGAAGATTAGCACCGGATTATATACTAAAGGATTGGC CATTTGGGACAAAGGGAGAAAAAGAGAGACAGAGAGCAGAGCAGAGATGTCGGTGAGATTACTTCATCTTCTTCCCCAAA ACAATTCCTTTTCTCGGAATCTCAAATTTCACCATCTGTTGACCCGACCCGTAACCCGAATCCAGTGCATAAGGAGAGAC GAGGAAGACGATGACATCATCAACAAGATGAGGATTAGGAGGCCGAAGAGGAAGAAGAAGATTATGACCGATTCGGAGCT AGCGACAGATTTGGCTAGAGAAATCGGGAAAGCGAACACAGTCGCGGAGCAGAGAAGAGAAGCGATGAAGAAGAGCGGTG AGATTCTGTGGGCAGAGTTTTGTAGGCATATGGATTTGAAAGAAGATGAGATGAAGATAAAATGGAACAAGATTGGAGAA GAAGAGAAATTGGTTCTGGTTAGAGAGTTTGTTGATGAATGGGCTGGTGATTTTCAACCTTTATCTATTACATCTGTTAA AGAAATGGTTGACCAAGAGTGTTTAGATTCTGACAAAACAATCGAATCTTCTTCTTCTTCGTCTATGTCTTCGTTTTCTG GCTTGTTTCCTGGATTGAAGAGAATCATTGGGTTTGAGTAGAAAGAATCTCCAATTTTCTTTTATAACATTGCAATACTG TAACATGTTTCTTTAGCTGAGAATACATTGGTTTCTTTAGCTGAGAATACTGTAACATGT ................................................................................ ..............N...........................i..................................... ..............................................N................................. ................................................................................ ..........N................i...................................i................ ................................................................i............... .............................N...............N....i..........N.................. ..................................N...N......................................... ....i...........................................................N............... ................................................................................ ....N...................................................N... Pos Score Pred ------------------------ 95 0.246 - 123 0.527 Yes 207 0.374 - 331 0.212 - 348 0.562 Yes 384 0.620 Yes 465 0.555 Yes 510 0.412 - 526 0.105 - 531 0.628 Yes 542 0.230 - 595 0.150 - 599 0.136 - 645 0.723 Yes 705 0.301 - 805 0.097 - 857 0.074 -

Explain the output -- Processing locus 986: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCTGACCGATAGATTGGCTGAGTCCTTTGAAAAAACTGTATAAGCCATTATTAACACTCAAATATACACACGTTCAAA AATTATTATCTTGAGCAATAAATGATCCCATCATCCAACCAATACGTCGACACTCACAGAGTCACAGAGGACAGAGCCAT ATATATACACGAACATACAAAGAGACTAGAGGACACGTTTCTATCTAGAGAGAACACAACACATCGAACCATGGAGAAAT CAACAAGAACCCTATTCATAACCATCGTGATAACCTCCATGTTGCTAGGGTTTGGAAACTCTGATCTTGCTCAGGACAGA GAGGAGTGTACGAACCAGCTCATAGAACTATCCACGTGTATTCCGTACGTTGGAGGAGACGCCAAGGCTCCAACAAAAGA TTGTTGTGCAGGGTTTGGCCAAGTTATAAGAAAGAGTGAGAAGTGTGTTTGCATATTGGTCAGAGACAAAGATGATCCTC AACTTGGTATCAAGATTAACGCAACCCTAGCCGCTCATCTTCCCTCCGCTTGTCATATCACGGCTCCTAACATCACTGAC TGTATTTCGATTCTGCATCTACCTCGTAACTCAACATTGGCTAAAGAGTTTGAGAACTTAGGAAGGATTGAAGACAACTA CAACTCCACATCTCCTACACAAATTCATAAAGATGGGACAGGAGGAGGAAAAGCCGAACCAGTGAAGAGTAATGGATGGA AGGAGAAGAGTTGGTTGGGTGTTGAGCTTTTAATATATTTGCTAGTTTCTCTCATCTTCTTTTAATTACTTCCTTTCTTT AATGTTCCTTTGCTTTATTATTACAATAACTTATAAGATATCTATTTATATATGAGACACATGCATGTGTTTGCGATCTA TATG ................................................................................ .....................i.......................................................... ......................................................................i......... ......................................N......................................... ................................................................................ .......................................................................N........ ................................................................................ ................................................................................ ................................N......................................N...N.... ................................................................................ Pos Score Pred ------------------------ 102 0.500 - 231 0.810 Yes 279 0.350 - 472 0.120 - 673 0.395 - 712 0.172 - 716 0.486 - 802 0.072 - 852 0.074 - 861 0.060 - 865 0.059 - 882 0.028 -

Explain the output -- Processing locus 987: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATACAATTCTTACTATTATAGTAACACCATAGATAGAGAATGTCATAAAGCAAAAAAATTAAATAGTAAATACAAATCAT TCTTTTTTCAAATATTTTTAACAAATCATTCTTGTATATATGTACACACACACCCCACAACATCCCTTCAACATATACGC GTCCTATTTCCACGTTCTCTCTATATTACATTTTTCCTTGAAAACATCTCGAGGTTCAAAATCTTTAACCCCTACAAATC AAGAAAAGGAGACAGAGGAGAAGAGAGAAAAGAAAAACGCAGAAACAATGTCTTTTTCTTCCCTCAAATTGCCAATCTTT CTCATTCTCTCAAGTCTTCTCCATGCGGCTATAGGGGAAAACATTGTTTGCGAGAATCTGCCAACAAATATGTGCTCGTT CTCAATATCCGCTTCCGGGAAAAGATGTATATTGGAGACGGCTAATGTCGCCGGAGAATTCACTTGCCGCACTTCCGCAG TGGACGTGGAAGGGATTGTAAACCACGTGGAGACCGACGAGTGCGTTTCCGCTTGTGGGGTTGATCGGAAAACCGTAGGG ATCTCGTCAGACTCCTTGATGGAGGCAGGTTTCGCCGCTAAGCTTTGCTCATCCGCTTGCTTGGACTATTGCCCTAACAT TCTTGATCTCTATTTCAACCTCGCCGCCGGTGAAGGTGCATTTTTACCTGATCTATGTGATGCTCAACGGATGAATCCCC AGCGTTCGATGATGGAATTCATCAGCTCCGGCGCCGCACCAGGCCCTGTCTCCGAAATTGCACCAGGCCCCACCTCCGAA GAAGTCTCATCTCCTGCTCTAGCTCCGGCTTCTATGTAAAATGTTCATTAATTCCACATGTGTTTAATACTTTATATTTA TATTTTCGATTGATGATGATCCATATATAATGGACCGTATCTTACATTTTCTATGATT .......................................N........................................ .......................................i........................................ ................................................................................ ...............................................i................................ ......................N..............................................i.......... ........................N...................N................................... ................................................................................ ..................N............................................................. ......................................................i....N..........i......... ........i..i.................................................................... .................................N......N................N...................... ............N..N.............N......................N..... Pos Score Pred ------------------------ 40 0.431 - 120 0.519 Yes 288 0.687 Yes 343 0.325 - 390 0.753 Yes 425 0.308 - 445 0.438 - 579 0.295 - 695 0.634 Yes 700 0.315 - 711 0.765 Yes 729 0.627 Yes 732 0.794 Yes 834 0.125 - 841 0.231 - 858 0.224 - 893 0.047 - 896 0.053 - 910 0.076 - 933 0.036 -

Explain the output -- Processing locus 988: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTTTAATGTTCAGAAAAATCGAACCGGATTAAGTTTTAGGAAAATAGAACCGGATTAATTTCGTGAATCAAACATACCG GATTAACAGAGCTTGATCTGAAACTTAACGAACTCGATCCATAAAGGGTTTTGTTTTGTGAGTTGGATCTGGCTTATTCC GATGATGACCGGAGATCTGAAACTTGATGAACCTTACGTAGGTTTGAAATTCGAATCAGAAGAAGAAGCTAAAGATTTCT ATGTAGAATACTCCAAAAGATTAGGGTTTGTTGTGCGTATGATGCAGAGACGAAGATCAGGTATTGATGGAAGAACACTA GCGAGAAGATTAGGTTGTAACAAGCAAGGCTTTGGTCCTAATAATCAACGATCATCTAGTTCTAGTTCTAGTTCACGAGA AGGTTGCAAAGCTACGATTTTGGTGAAAATGGAGAAATCTGGGAAATGGGTTGTTACTAGATTCATCAAAGAACACAATC ACTCTCTTCAATTCATTGGGTCTTCTTCTTATGATTCTTTTGCTGATAAGGAAAGGAAGATTAAAGAGCTTACGGAGGAG ATCGAGTGTCAGGACCGATTATGCGATGTGTATCGGGATCGATTGGTGAGTTTTATCGATAATGTTGAACATTATACGGA AGAATTGTCACTTAAAGTTCGAGATATTGTGGAGAATGTTAAGAAACTTGAATGTCAAATACAAACAAGAGCTTGATTCA TCTATAAGCATTTGTCGAAAAATACCGAAGATTGCTCCTCCTTAGATCATAGTTTGTCCTAGCATTGAACATTCAATTAT TTTAGCAGAAAACTA ......N......................................................................... ................................................................................ N.....................................N..N........................i............. ................................................................................ ............................i................i.................................. ..............................N................................................. ....................N....N...................................N.................. ...................................N...............N............................ ................................................................................ ............... Pos Score Pred ------------------------ 7 0.402 - 162 0.363 - 165 0.516 Yes 187 0.113 - 241 0.333 - 279 0.214 - 282 0.425 - 307 0.732 Yes 429 0.637 Yes 446 0.622 Yes 511 0.100 - 581 0.259 - 586 0.174 - 622 0.075 - 676 0.134 - 692 0.285 -

Explain the output -- Processing locus 989: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAAAACCATACTATTAAAAACCAATTAAAAAACAAGATTAAAAAGTTATCGAGCCGTTACACCTTAATTGAATTATTCT GTCTTCGTCCTTAAGTCATAACGGTCATATTTTCAAAAACAAAAACGACCGTTAATCCCAACAAAATCTCTACTTTCTCT TACCTCAACTTTGTTTCTTATGTATGCATATAAACCCCAAATCAAAGTGAACTTATCAAGAAACACAAAAACAAACATCA AAACCCCAAAAAAAAACCAAAGTTCTCTTCTTGAATTCGAGAAAATGGGAATGTCGAAATCGAAAGGCAACACACACAAC ATCTTCCTTCTCTGCAACTACATCCTCTTAGGCTCAGCCTCAAGTTGCATCTTCCTCACAATCTCCCTCCGTCTCTTCCC ATCTCTCTCCGGTCTCTCCCTTATCTTCCTCTACACTCTCACAATCGCAACCGCGGTATCCGGCTGCTCGATCTTCGCCT CCTCCACATCCGCCACCGCGAGCGATAGATTATACGGTTCACACATGGTAGCCACAGTCCTCACGGCCATTTTCCAAGGC GCTGTCTCTGTTCTGATATTCACGAGAACAGGGGATTTCCTTAGGTTCTTGAAATCTTATGTTCGGGAAGAAGACGGTGA AGTGATACTTAAACTCTCTGGTGGTTTGTGTGTATTGATGTTTTGCTTAGAGTGGATTGTTCTTGTGTTAGCGTTTTTGT TGAAGTATAGTGATTATTTGGATGAGAGTGTTGTAGATGATGATGATTTTAAGGTGAGGAGGCAAGAAGAAGATCTCAAG GATTGGCCTTCTTACCCATTTCAACTCAAGATTTAATTTCCAATTTCGTCATTTTATTCTATTCATTTACACGTGTGTGT GTTGGTTCTTGCTTGTAATGTGATTAATGTCACACTTTAAATGGTGTACAAATAA ................................................................................ ................................................................................ ...................N...N........................................................ ............................................i.....i............................. ................................................................................ ................................................................................ ............................................N................................... ..........................................................N..................... .....................................i.......................................... .....................N..............N..N..N..................................... ................................................................................ .................N........N.............N.............. Pos Score Pred ------------------------ 180 0.256 - 184 0.293 - 285 0.706 Yes 291 0.626 Yes 525 0.497 - 619 0.093 - 678 0.521 Yes 742 0.249 - 757 0.077 - 760 0.094 - 763 0.062 - 898 0.037 - 907 0.053 - 921 0.071 -

Explain the output -- Processing locus 990: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 991: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGGTGTCCACATTGAAGAGTCTTTACTTCATGATTCTGATTTGCTTGAGTTTAAATACCTTAGAGTATTTTATACGGTAT TTGTGTGGTAAATCTGAATGAGTGTTTCCAATTCGACTTGTGCTAAAACACAGGTCTTCTAAAGATGTCCAGGATACACA TAGTTTGGTAATGCACACATACTGTTCTGATGATTCTAGCTCACGCGTGCATCATTTAGGCCTACACAAAGCTCTCTGTG TATTGATGGGATGGAATTTCTCAAAGGCTCCTGATAATTCAAAGGCATACCAGAATCTACCTGCTGATGAGGCAGCAATC AACCAAGCCCAGCTAATTATTTGGCCACCTCATGTTATTGTTCAGAATACCAGTACAGGGAAGGGAAAAGAAGGGCGCAT GGAAGGCTTTGGAAACAAAACAATGGATAACAGAATTAGAGAACTAGGACTCACTGGTGGGAAATCGAAGTCGTTGTATG GAAGAGAGGGTCATTTGGGAATTACCCTGTTCAAGTTTGCTGGAGACGATTCAGGGCTCAGGGATGCTATGAGAATGGCA GAATATTTTGAGAAGATAAACCGTGGGCGTAAGAGTTGGGGGAGGGTACAGCCACTCACTCCAAGCAAAGATGATGAGAA AAACCCTGGTCTTGTCGAGGTTGATGGGAGGACGGGAGAGAAAAAGAGGATCTTTTATGGTTATCTCGCGACTGTAACGG ACTTGGACAAAGTCGATGTGGAGACAAAGAAGAAAACCACAATCGAGAGCCTTAGAGAACTCACAAGAACAAAGTAAATT ACAGTTTTCTTCTCCCACGAAAGGTACACTTAACTTTCGAGGTATTTGTTGCCTATTGAATCTGGATTCTTAGTCGCAAC CTAAAAAACTCGAAAG .............................N.................................................. .................N..............................................N............... ..........N..................N.................................................. .....i....N.......................................................N............. ...............................N..............................................N. ......................i......................................................N.. ...............................................................N....i.....N..... ......................................................................N..i...... .......................N................................N....................... ...............N................................................................ ................................................................................ ................ Pos Score Pred ------------------------ 30 0.103 - 98 0.440 - 145 0.391 - 171 0.386 - 190 0.297 - 246 0.624 Yes 251 0.412 - 307 0.344 - 352 0.266 - 399 0.428 - 423 0.658 Yes 478 0.242 - 544 0.336 - 549 0.506 Yes 555 0.486 - 631 0.230 - 634 0.538 Yes 664 0.176 - 697 0.186 - 736 0.234 -

Explain the output -- Processing locus 992: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTAAATCTGAAGATGAATCACACTAGGAAGTGTTTTAGATCCCAAAGCATTAATCCTGTTTTCTGTAAAAATGCCTTCTT TGTCATCCTAATGATTTTTGATTTATGAACTTTGGTATAGGGATTTGGAGTTTGTGTACAAAAAGACTGTTGTTGGAATG TGCAAATGAGACAATATTGGAAATGTTATTTGCAGCAGAAGGAGGAGGATTCTTCTCTTCATCAGCTTCTGGTTATAGCA ATGGTTTAGCTCTTCTTCTATTAGGTCAAAAGACTGAACAAAAACCAATCAAAGTTTCATCACAATGGAACCATTATCAT TTAGTTCTTGAAGATTCTGATACCGGGTTTCGGTTAGATTCGTCTAAGAATTGGCTCTCTTCTGCTTGCACTTCTCTCAT ATGTTTTGGTCGAAAATCCGAAAGACTGGAAAGCGAGGGGAAGAAAGATGAGGCACCGAGTGTGGAGGACTATAATAACT GTGAAGTCACTAATAGGTTTGCACTTAAAAGCAGCTTGAAGAAAAGATCGTTTAGTGATGTTGTGATTGGTGATGATGAT GTGAGTAGAGATGGTGTGGTGGATCATATAGATAGAAGGAAAGTGCAGTGGCCTGATACATGTGGTATTGAGATTGCTGA GGTCAGAGAATTTGAGCCTAGTGAAGTTGATGAATCAGAAGATGAGTTTCATCATGGAAGTGGAAAAAGCTGTATGTGCA CAATCATGTGACCCATTGGAAGTTAAAAATTGTTTGCAGACAAAGGGCTTTTCATATAGTTGATGTAGCTGCTCTCTTAT CATGTGCACCTTTGTTATTATATATAGAAA ............N.........................................................i......... ..........N.............N....................................................N.. .....N................N......................................................... N...............................................................N............... ................................................................................ N..............................................N................................ .........................................................N..............N..N..N. ..........N................................................N.................... .............................N...........N...........N...................N...... .....N........................................................N................. Pos Score Pred ------------------------ 13 0.216 - 71 0.513 Yes 91 0.084 - 105 0.431 - 158 0.155 - 166 0.477 - 183 0.454 - 241 0.215 - 305 0.207 - 401 0.239 - 448 0.411 - 538 0.218 - 553 0.193 - 556 0.226 - 559 0.130 - 571 0.109 - 620 0.252 - 670 0.098 - 682 0.134 - 694 0.329 - 714 0.083 - 726 0.165 - 783 0.041 - 802 0.066 -

Explain the output -- Processing locus 993: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGGTCGGTGAAAGAGTCTCGAGACACGTGGCACAAGAACCAGCTTGGGGAATCTCGAAAACTCTCTTACTCATCGCCAT TCCCACACGTATGATGAATGACGTGTGCCAATGGATCACATTACCCAAACCTTATATAAACCCACCGAGAGACCCAACAA AACTCTACCAGACAAAACAATGGAAGCTATCTCTCAAACCTGCACAAAGATCATCACCAACCCATCTCAGAGGATATATA AAACCTCATCTCCAGTCACGGCAATTCCACAAACAAAGCTGAGCCTTAAAGTCAAGAAGGAGAGGTGGGTTGCTGCTGCT AGATGCGTGGCTTCAGGATCGGATTATGCTGCGGCAATGGAGCCAATTACGCCGGAGGAAGAGGAGGAATTGACTCAGAG AAGAGGGATATGTGGTGGAGAAGTAAACAGAGGAGTATGGGAGCTCTTGGAGTGCCTTGAGAAAGAAGCTATTATGGGGA ATGATGATGGGAGAGATCCTAGAGATTACAATAGAAGAGCAAAGATTTTTGACAAGAGTTCTAAGATCTTCAAGAACTTA AATGAACAGAGAGATCAGGCTCCTGTTGATTACCTTGAGAAGGAAGCTATCATGGGGGCTGATGATGGGAAGGACCCTAA AGATTATAACAGAAGAGCAAAGATTTTTGACAAGAGTTCTAAGATCTTCAAGAACATCAAAGAACAGAGAGAGCAGTCTC CAGAATGAATTACGAAAATGTAGTTTGTTTGTTTTTTTGGTATGATAAATGTAGTCTGTTTGTAATGATCTCATTAGTGG TTTGAATCGTTTGATTCCTTGTACCGT ................................................................................ ..........N..N...i............i................................................. ...................i............................................................ ................................................................................ ..N......................N..........N........................................... .........N..........................N....................................i...... N..N..N......................................................................... ................................................................................ ....N............N.......................N......N...............N............... ........................... Pos Score Pred ------------------------ 91 0.101 - 94 0.297 - 98 0.673 Yes 111 0.824 Yes 180 0.773 Yes 323 0.312 - 346 0.293 - 357 0.332 - 410 0.466 - 437 0.137 - 474 0.624 Yes 481 0.407 - 484 0.247 - 487 0.262 - 562 0.263 - 612 0.804 Yes 622 0.191 - 625 0.349 - 725 0.282 - 738 0.095 - 762 0.036 - 769 0.053 - 785 0.039 -

Explain the output -- Processing locus 994: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTTTAATGCATCAGTTAGTCAGTGACCATCAGCTGATGCAATGGTGAAAAAGGGTTTGGTCACAATTAACACACAGCTC TGTAGTAACTGTAACGGTTAGCTTCTTCATTTTCACGAGCTTTGTTTCTATGGTAGTCACGTTTTAATTCATGGGAAGGA TTTGTTTGGATTCAAGATGCCACTGACCTATCTTCTCTTATTTATTCTTGCAAATTCTCATCATATGATTAATCATCACA GAAACATAATTAAACTTTTTTGTATAACCAGAATGTCTTCAGTAATGATGTGTTGTTGTTTGCTTCTCTTGTTTGGTTTG TTGTCTGAAGGTAGAGAGATTCTGGTGGGAGGCAAATCTAACACATGGAAGGCTCCAGAATCTAGAGATGAAACACTAAA CCAATGGTCAGGCAGAACAAGATTCAAAATTGGAGATTCTCTCTTGTGGAAATACAATGCGGAGAATGATTCAGTGTTGC AAGTGAGACAAACAGATTACGAGAGATGTGACAGATCAGAGCCAATAAGAGGATACAAAGATGGTCACACGAATATAGAG CTGAAGAGGTCAGGTCCTTTTTACTTCATAAGTGGAGAAGAAGGACATTGCCAAAGAGGAGAGAAGCTTCGTGTTGTTGT CTTGTCTCCAAATCACAACAGAAGCGTTGTTGATGCTCCTGCTCCTGTAAACATTGTCTTGTCTCCAAATTACAACAGAA GCGTTGCTGCTGCTCCTTTAAACGCTCATATCATGAACAAGGGAAGCCTGAACACAGCATGGAGTTTGCTTCTTCTTCTT CCATTAGGCCTTTTGGTTTAGTCTTGTGAATTACCTCTAGCCAATTCGGTTCGATTTCGGTTTGTATTTTCCGGTTTACT TGTTACTTTGGCTTGTGATTTAGTCTTGTTTCTTAGCCAA ......N.............................N....i...................................... .................................................N....................N......... ................i...............................................N............... ................................i...........i..i................................ ............................................N......................N............ ...N....................................................N........N.............. .........................i..................................i................... ................................................................................ ................................N............................................... ................................N.........................N..................... ................................................................................ ........................................ Pos Score Pred ------------------------ 7 0.276 - 37 0.276 - 42 0.512 Yes 130 0.082 - 151 0.123 - 177 0.503 Yes 225 0.140 - 273 0.672 Yes 285 0.534 Yes 288 0.528 Yes 365 0.358 - 388 0.218 - 404 0.398 - 457 0.405 - 466 0.451 - 506 0.505 Yes 541 0.536 Yes 673 0.251 - 753 0.386 - 779 0.462 -

Explain the output -- Processing locus 995: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCACGAAGAGCCACGTCAATAATCCGAGCACTTATTTTTCATTGGCCAGTGCCCCAAGGCCCAAACACTCATTTACCA TTATTTCATGGGCCAACATAGAGGCCCAATTTAGCGCTAAAATCCAAGAATTACATGATACACCCGAATCTTTCTCTTTT TCACGAAACATTCCAAAATATTCTCGAGTCTATTCAGATTCACTCTCTTGTCACTGTTAGATCTTCCAGTTTCTCCGATA CCTTTAACGGTAACTTTCTGGTGCTCCACTGCAAAATCAGCGGTTATAAAAAAAAAACTCAAAACCTTCTTCTCAACACT CCTTTGCTTGATCTTCTCCCTCTCTCGTTACTCTCTTCGGAAATGATAATGTCTCTCCCTCAGTGTTCTCATCTGCGTCT CTCTATTCTCGCTGCGACGGCGGCGATGTTAATGGTGATTACGACTGCGCAGATCGGCGGTCAAGTGATACAGGTGGAGT TATGGTGTGTGGCGAAGAACAACGCGGAAGATTCGTCGCTACAGACGGCGATTGAGTGGGCTTGTGGTCAAGGCGGAGCC GATTGTGGTCCGATTCAGCAAGGTGGACCTTGTAACGACCCGACGGATGTTCAGAAAATGGCGTCGTTTGTTTTCAACAA TTATTATCTGAAGAACGGCGAGGAGGACGAAGCTTGCAATTTCAACAATAACGCTGCTCTTACTTCACTCAATCCAAGTC AGGGAACTTGCAAATATCCTTCGAGTAAGGGAGCCAACAATGGTCGATTAGCGGATGACACTTCAATGGGTGCAGGACAA GCAGATATGAGCCGTGGTGGCAGACCGATTTCCAGCAGTTGGATGGTGACCTTCATCGGTTTCGGTAGCTTATTGACGAT GACATGGATAATTCATCATCTTTAGGCTCACACGCATGATGCTCTCATTGATGATATGAGAGACGGATACTATTTTGATC TCTCTTAGTTTACCTTTGAAGATAAGCGTAATATCTAATTCTTT ................................................................................ .......N..............................................N......................... ................................................................................ ................................................................................ ..........................................N.....i............................... .........................i.....i................................................ ..............................................N..........i...................... ................................................................................ .......................................i..............N..........i.............. ......N...................................i...................................N. ...N...............................N..N...........N....N........................ ............................................ Pos Score Pred ------------------------ 88 0.131 - 135 0.320 - 363 0.369 - 369 0.764 Yes 426 0.702 Yes 432 0.611 Yes 482 0.553 Yes 607 0.245 - 618 0.802 Yes 760 0.707 Yes 775 0.183 - 786 0.750 Yes 807 0.288 - 843 0.795 Yes 879 0.428 - 884 0.284 - 916 0.069 - 919 0.087 - 931 0.041 - 936 0.131 -

Explain the output -- Processing locus 996: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTGTAGAATAAAGTAAACGCATTATTAGCGACTTTAAATATATAGAAAAGTGCAAAATAAATAGGAGGGGCAAGATCAA TAAAACTTCTTCTATAAATAGAGCTGCATGGGGGAAGACACTTTATCACATCCAAAACCAAAAGCAACACAAACACACAC ACCAAAAAAAAAAAAAAAAGCATTTACTTTTAACTCAAGCATGGCAATGTTAAAGAACAAACACATGACCGTCTCATTGA TCTTGGTGTGTCTCGTTGTGGTGTCTCCAATGGCTGAGGCTCAGCTTGGTCTTGGTGGTAGTGGTGGTCTTGGTGGTCTT ATTGGGGGTCTTGTTGGGGGTCTCGGTGGTCTGGTTGGTGGTCTTGTAGGGGGTATTCTCAATCTCGTCAATATCAATGG GGTTGTCTTTTGCTCTCTCAATGGTGCTCCCAGTGGCACTTCCACTCCTGCTTTCGCTAATGCAGGAGTTGAGCTCCAGT GCGGAAGGCAAAACAGGGTCGTTTCAACAGCGACCACGAATGCTGCCGGATTATTCTCGCTTCCTACGGACTCAATTCAG ATGTTACTTTCCACACTTCTCTCTGATTGTCGGGTCGTCGTCACGACCCCTCTCTCCACCTGCAACGCCAACCTCCCTTC CGTTGGCAACCTCGTCTCACGGTTAGCCATGATCGGCAATAGTCTCACCGGCCTCCTCAACATCATCAGTATCATCCCTG CCGGCTTTGGTCTTCTTAACTGATTCCACACTCTATACATACTCTATGTTCGGTTACAGTGTGTTTCCCTTTTGTTATAT GTTTTGTAATCATGACCATTTACGTTTGCTTGCGTATGCATG ................................................................................ ...........................N.................................................... ........................................i.....i.................i............... .............................i.................................................. ............................................................................i... ....................N......................................N.................... .......................................N........................................ i............................................................................... ............................i................................................... .............................................N................................N. ...........N.......................N...N.. Pos Score Pred ------------------------ 108 0.447 - 201 0.747 Yes 207 0.661 Yes 225 0.825 Yes 270 0.817 Yes 397 0.570 Yes 421 0.201 - 460 0.194 - 520 0.358 - 561 0.637 Yes 669 0.641 Yes 766 0.126 - 799 0.064 - 812 0.058 - 836 0.039 - 840 0.043 -

Explain the output -- Processing locus 997: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACAGGATTTACAAAAGTTATTATCTTTATCGATTTCTCTTCTTCCTAGACCCAACAGACGAAGAGAATTTGTTGTTGGTT GTCTCTGGTCTCTTCGTCTAGGTTTTTTTTGGGTTATTAAAGATGGTGAGGATCGTGAGAAGTTGTTTACAGTCCATGTT AAAGCTCGTGAATTCTCTAATTGGGATGGTTGGGATTGCTATGATTCTCTACGCTGTTTGGCTGATTAGGCAATGGCAAG AACAAATGGGAAATCTTCCTTTTGCTGATTCTGATCACCCTGTTCCGTGGTTCATTTATTCATTTCTTGGTCTCGGGGCC ATTCTCTGTGTTGTCACTTGTGCCGGGCACATAGCTGCTGAAACCGTGAATGGATGTTGCCTTTACTTGTATATGGGATT CATAGTCTTGCTAACTATGGTTGAAGGCGGTGTAGTTGCCGATATCTTTCTAAACCGTGACTGGAAAAAGGACTTTCCAG AGGATCCAAGTGGTGCTTTCCACCAGTTCTCTAAGTTCATCGAGTCAAACTTCAAGATTTGCAAGTGGATAGGTCTCTCT ATTGTCTGTGTCCAGGGTCTATCAGTTTTAATAGCCATGTTGCTCAAAGCTCTTGGACCTCACCCTCACCGACATTATGA TAGTGACGATGAATACAATGTAAGCACAGTTGCACTCCTGCAGGATGCTCGTCAGCCGCCTCCATACGTTGTTGGAGAAC CCATGTATGGAGCCAAACCGGGAGCTTGGACAGTTAGATTAATGAAAGGGCAAACCGGTGAGAGGTGAAAACAAAGAAAC TCGTAGAAGAAAATGGCAAAGCAGAGTCTGCTCTTTCCATGTGTGCATTTACTTTGGCTTCTTATTGCATCTCTTTCTTT CACTATT ................................................................................ ..........................................N................................N.... .........................i..............N...............................N....... .....i.......................................................................... .................................................i...N..................i....... ................i............................................................... ................................................................................ ....................................i.......................................N... ........N........i..........................N................................... ..i...i..................................N...................................... ............N.........................N......................................... ....... Pos Score Pred ------------------------ 123 0.313 - 156 0.101 - 186 0.554 Yes 201 0.309 - 233 0.441 - 246 0.679 Yes 370 0.533 Yes 374 0.324 - 393 0.569 Yes 417 0.790 Yes 597 0.699 Yes 637 0.355 - 649 0.476 - 658 0.522 Yes 685 0.252 - 723 0.810 Yes 727 0.537 Yes 762 0.176 - 813 0.163 - 839 0.064 -

Explain the output -- Processing locus 998: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAACATTAAATTATTATATGCTTTTGTTAGAAATATTAAAGTTTCAAACTTTAAAGTTTAAGAACACTAATAGTAAGTTT TGGGTCAATTTTATTTGTTAAAGAAGTTTTGGGTCCATTTACATGACATTATTTATATCATTTGTCAAAAGGCCCAAACG TCTTTTTCAGTTGCTCTTTCTTGGTGGGAACTGTTTGAGCTCGAGTTCTATGATGAACGTGAGCCGGCCGGCGATTCACC CGGTTGATGCTCTTCCGGTGGCTCCTACTGCCGGAGCGATCGATAGACCACCAGTGAGGATGAAGGATGTGCAAGGCATG CCCGGAACCACCGGCGGTCTCATCCTCCGTCTCTCTCAGTTCGTTCCCGCTCTTATCTCCGTCTCCGTCATGGTCACTAC CTCCGACTTCCGCTCCGCCACCGCATTCTGTTGCTTGGTTCTTGCGGTTAGCCTGCAAAGCCTGTGGAGCTTGTCTCTTT TTATTATCGATGCTTATGCTCTTCTGGTGAGAAGAAGCCTTCGAAATCACTCCGTTGTTCAGTGTTTCACTATTGGAGAT GGAGTCACATCGACGCTCACGTTTGCAGCTGCATCTGCTTCTGCTGGCATAACTGTACTCATCAACGATCTTGGACAATG TAACGTGAATCACTGCACAAGGTTTGAGACTGCCACAGCAATGGCCTTCATCAGCTGGTTTGCTGTGTCTCCTTCCTTTA TTCTCAACTTCTGGTCCTTAGCCACCCACTAAATATATAGCACACAAGTTGAGTTCAGAGAAATCCATCTTTCTAGTATA ATGTAAATAAAGGGTGGAGTATAATGTATCGCTTGATTAGCAAATGTTTTG .................N.............................................................. ..........................................N..................................... .................................................N..i........................... ......N....................................................i......N..........i.. .....................................................................N.......... ................................................................................ .........N.....N..............................................................N. .............................................................................N.. ........................................i....................................... ................................................................................ N......................N...................N....... Pos Score Pred ------------------------ 18 0.288 - 123 0.236 - 210 0.473 - 213 0.854 Yes 247 0.244 - 300 0.695 Yes 307 0.217 - 318 0.792 Yes 390 0.313 - 490 0.138 - 496 0.191 - 559 0.478 - 638 0.384 - 681 0.657 Yes 801 0.054 - 824 0.072 - 844 0.044 -

Explain the output -- Processing locus 999: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCTAGTACAACACCCACAAAAGAGCCCATTAACTTCGTTGTACAGAATGCAACCATTCCGGGACCATTTATTTTAAAGCC CAACCAGAGGCCCATTACTGACCCTTTCTAGTTGAGGAACAATTAATAACTTTTGTCCCAATGTTGTCAACTTTGTTCAG AAGAAGTCTCTCTCAGTCTAGGTCACTCGAAACCATGTCTTCTTTGACAAGCGAAAATGCTGAGACTAAGGTAGGCGTCT TCTGGGACGTGGAGGAATGCCCAGTCCCTGATGGTGTGGAGCCATCTGTGGTTTGTGAGAATATCAAACTTGCTCTTGAG AAAAAGGGTTATCGTCCATGTAATGTCTCAATCAGAGTTTATGGTGAGAGGACTAATAAGTTCAAGGATGACTTTCTCCT CGCTGATATCATGTTCTTACCCGCAGGAGATGAAGGAGCGAGATTTATGAGGATGTGTAATGATTTCTTTTGCTGGGGAA ATGACAATCGTAAATCAACTTTGATGGTAATGTCACGAGACAGCACTGAGTTCGCCTCTTCTTTCGTAATGTACAAAAAC TTGAATTTCAACATTCTCGTAGCACAGCCAGAAAACGTAGACAGAAAATGTCCTGTCTGCAGAAAGCCTTTGGAAATGAT CATCACTGACGAATGGGTTTGGGAAAGCCTCTCTGCTGGAGGAGACCCTCTCCCCAAAACCCAAGAAGAACCAGACCTTA GTAACTCTTGTCATTAACTATGCTGCTTTTATTACTAGTTGTTATTTGTAATGTTTAAAATCGATTGTGAATTCTTCTCT CCATCACATTACCACCTATGTACAACCAGATCATCG ..............................................N................................. ............................................................i................... ..................................i.....................N....................... ................i.............N................................................. .................N....N.................N..........................N............ ..........N..................N................N.....N......N.................... N......................i.....i......................................i........... ...............................................i...........................i.... ............N................................................................... ...................N..............................N............................. .................N.................. Pos Score Pred ------------------------ 47 0.317 - 141 0.577 Yes 195 0.659 Yes 217 0.327 - 257 0.529 Yes 271 0.151 - 338 0.240 - 343 0.311 - 361 0.180 - 388 0.323 - 411 0.167 - 430 0.185 - 447 0.430 - 453 0.309 - 460 0.196 - 481 0.238 - 504 0.509 Yes 510 0.598 Yes 549 0.677 Yes 608 0.733 Yes 636 0.599 Yes 653 0.175 - 740 0.255 - 771 0.141 - 818 0.057 -

Explain the output -- Processing locus 1000: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATTCATTAGACGGTTAGAGAGTCTTCATCATTGTTTAAACATTTCCTTAATTTGACATTTAGCCATCAAAACGGAAA TTTAGTTGGAAAATAAAGTAAATGACGAAGTTACGTAAATGCCCCCGGTTCTTTCATATTTCTTCAAAACAGTTTTTGGC CAATGGAGAAAGAAAAATTGAGAGTCGCCACAGCTATCACTCTGCTCTGTCACTTTGTCTTTTACTCAGAGAATCAGAGA CAGAGAGAGAAAGAGAGAGAGAGAGAGCGAGGGGAAGGGAAATGGAGAAGGCATTAACGAAAGTGACGAGCTTTAAAGTG GGAAGTTCATGGATCTCTAAGAAAGCTAAAGAAGAGCTCTCAAACATCACCAATGACCTCACTACATTCTCCAGTACGGT TGAAGAGAAAGCAAAATGGATTTTCAACAAGCTTAAAGGAAAACCACTGAAAAGCTTACCAGACCTCCTCAAAGAATACA ACTTACCACCAGGACTCTTCCCACAAAACATAATCTGCTACGAATTCGATGAGACGAAGAATAAACTCACCGTCTTCTTC TCTTCACCATGTGAAGTCACATTCAAAGATGGATCAGCGATAAGATATGCAACGCGTGTGAAAGGGATTTTGCTTAGAGG GAAGCTGATGGGTGTGGAAGGAATGAAGACAAAAGTGTTGGTGTGGGTTAAAGTCACAACAATCTCAGTCGAGAGCTCCA AGTCTGACAAGCTCTGGTTCACTGCTGGTGTCAAGAAATCTCGATCTAAGGATGTTTATGACACACCTCATGATGCTATC AAAGTCATTGGAGAGTTCTAAGGATTTTTTTCACAATTGGTTATTATCATATTTGATTCATGAATTTATTTGGGGTTATT TGCATTTTGGGAAGAGGTATACTTAAAGGAAATGTGTTTA ................................................................................ .....................i................N......................................... ..i............................................................................. .........................................i...................................... ........N...........................................N........................... ...............i................................................................ ................................................N............................... ........i...................N.................N................................. .......i..............N......................................................... ...................................................N.....N...........N..N....... ...........................................................N.................... ...............................N........ Pos Score Pred ------------------------ 102 0.551 Yes 119 0.154 - 163 0.782 Yes 282 0.801 Yes 329 0.262 - 373 0.396 - 416 0.750 Yes 529 0.358 - 569 0.505 Yes 589 0.367 - 607 0.321 - 648 0.512 Yes 663 0.338 - 772 0.172 - 778 0.100 - 790 0.096 - 793 0.166 - 860 0.090 - 912 0.071 -

Explain the output -- Processing locus 1001: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTTGTTATAAAAATTACTCCTAATTAATCAATTAACAATTAATGAGAATTAATGCAAAAAAACATAAAAATAATTGAG AGTCATTGTGGTGAGATATAAAGAAGATTTGTAACACTAAAAATATGGATGGTGAATAACATAAAACTCATTCCAAAACA AAGAAAAGAGAAGTCACAGGTGATGATGGCCAAAGAAGGAGAACATTCCGGAGCTAAGGAAGTGAAACTTACCAAACCCA AAATTTCCTTGTCGGATCTCACCTTTGTCATTTACGTAACTACCCGAGAAAAGCTATCATCTTCTACCATAAGTTGTGAG GAGATACCACTTAAGAAATGTGGCGCGGAGCTAGAGTGTGGCTCCAATGATCGATTCCATATACAAGTCGAAAACTTGAG CCATATGGGAATCTCCAAGTCTAGAATGGCTGGTGTAAAGATCGAGTGCGAGGTCATTTATAAGGAAGACTCGGAAGACG AGGCGGTAGTTAGAATCGTTAATGCTGAAAGGCCATTAGGCACAAACTTTGGATGGCTGAACAACGATCTTCTTGCTCCA CTAGCTCATCGTGGTCTAACAGCCGAAATCCAGCTGAGTTTCAACAGCGAGTTATTGGACAAGATCGACCTCCAGATATT CCACCGAGGCGAGGGGAGATATTTTTCCCAAGACGATACTCTTGGTTATCTTCAATGTTTTTTCCCATCTAAAATCTAAG TATTTTAGTTATGCAAAGCCTATGAAATATTTAAAAGTACAATTTGTATGCATTTTGTTTGGTCGATTATATGATATTTA AATAAGAATATATTTCTA ...........................................N.........N.......................... ............................................i...i............................... ......................i..i...................................................... ................................................................................ .................i............................N................................. ....i....................i...................................................... .....................N..............................i........................... ................................................................................ ......................................................N......................... ..........N..........N.........................N......................N......... .................. Pos Score Pred ------------------------ 44 0.416 - 54 0.419 - 125 0.860 Yes 129 0.610 Yes 183 0.533 Yes 186 0.781 Yes 338 0.648 Yes 367 0.450 - 405 0.519 Yes 426 0.851 Yes 502 0.195 - 533 0.628 Yes 695 0.312 - 731 0.172 - 742 0.043 - 768 0.040 - 791 0.041 -

Explain the output -- Processing locus 1002: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCACTAAGACCTATTATGGTATGTTGTATTGTATGACCCATTTTTTCAATTATTTTTTTCGGCCTAAACCAGCCTTTTAC AGAAGCAAAAGGAAAAACTGAAACCCGAGACATCTAAACCGGATTAAACCGAATACCATGCCATTTCTTTTTGATGAGTC ATCATCACCATCCTTTAGATCCAGAAATCAAAATTGCTTACTGCTTCATTTGTTCTGCTGTTCCCGGCCAATGAAGATTC TCGTCGCCGTTAAACGTGTCGTCGACTACGCCGTCAAGATCCGAGTCAAACCCGATAAGACTGGTGTAGAAACCCAAAAT GTGAAGATGTCAATGAATCCATTTTGTGAGATCGCGCTTGAGGAAGCGCTTCGAATCAAAGAAGCTGGTTTTGCTAAAGA GGTTATTGCAGTGAGTATTGGACCTTCACAATGTGTGGATACACTTCGTACTGGTTTAGCTATGGGTGCTGATCGAGGAA TCCATGTTGAGACCAATTCTATTTTTCTTCCTTTGACGATTGCTAAGATCTTGAAGAGTTTAGCTGATGTTGAGAATCCT GGATTGATTTTCTTAGGGAAACAGGCGATAGATGATGATTGCAATCAAACAGGACAGATGGTTGCAGCTTTACTTGGTTG GCCACAAGCAACGTTTGCATCGAAGGTTGTGTTGGATAAAGATAAGAATGTTGCAACCGTGGATAGAGAAGTGGATGGAG GTCTTGAGACTCTTAATGTAGACTTACCCGCTGTGATAACCTCTTGATGTTACAATGCAGAACTGATTTGAGGCTGAACC AACCAAGATATGCATCACTCCCTAACATAATGAAGGCAAAATCAAAGCCTATAAAGAAAATGACGG ...............i....N...........N............................................... .........................................................N...............N...... ......................................................................i......... ..............................................................................N. ......i.....i................................................................... ..............................N..............................i.................. ...N..............................................................N............. ...............................N..N......................i...................... ...............................................N..........................N..... ...............N..............................N.......N......................... .........N...................N.............................N...... Pos Score Pred ------------------------ 16 0.523 Yes 21 0.243 - 33 0.243 - 138 0.149 - 154 0.264 - 231 0.652 Yes 319 0.294 - 327 0.793 Yes 333 0.549 Yes 431 0.337 - 462 0.548 Yes 484 0.170 - 547 0.304 - 592 0.184 - 595 0.135 - 618 0.832 Yes 688 0.223 - 715 0.331 - 736 0.417 - 767 0.267 - 775 0.263 - 810 0.069 - 830 0.056 - 860 0.055 -

Explain the output -- Processing locus 1003: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTAAGGATATAATATTGAAACTTGCTACAAAAATTTGACTAGAGAGAAATAATAAAATGAGGAGGAGGAAATAACCAAAT TTCGGGTGGGCTAAGACTTGATTACAATTAACAGCCCATTGGGCTTTTATGATATCAGTTTTAGAATTAGGGTTTTTGGG CAGATGCTCATTTATAAAGAAGAAGAACCCTAGCCGCTTATATTTGGTTGAGGTTTCCACCGCATTGTCTCTCTCCAGAC GAAAAATGGGTGCTTTCAGGTTTCACCAGTACCAGGTTGTGGGAAGAGCTCTCCCGACAGAGAAGGATGTGCAGCCTAAG ATTTACAGGATGAAGCTCTGGGCTACGAACGAGGTTCGTGCCAAGTCCAAGTTCTGGTATTTCTTGAGGAAGCTCAAGAA GGTGAAGAAGAGTAATGGACAGATGCTCGCCATCAACGAGATCTATGAGAAGAACCCAACAACGATCAAGAACTTCGGTA TTTGGTTGAGGTACCAGAGTAGAACTGGGTACCACAACATGTACAAGGAGTACCGTGACACCACTCTTAACGGAGCTGTT GAGCAGATGTACACTGAGATGGCATCCCGTCACCGTGTCAGATTCCCTTGCATTCAGATCATCAAGACAGCCACTGTCCC AGCCAAGCTGTGCAAGAGAGAGAGCACTAAACAGTTCCACAACAGCAAGATCAAGTTCCCATTGGTGTTCCGCAAGGTTA GACCACCAAGCAGGAAGCTGAAGACGACTTACAAGGCCAACAAGCCCAACTTGTTCATGTAAAACGTCTCTGATTGACTC TTTTTTGGGGTGGAATCGATGTTGAAGCTGTGCTCTCTCCCGTTTTGGATTTTATCGGTTATTTTTGTTTTTCAATATGT ........................................................N....................... ................................................N............................... ...N............................................................................ .....i............................................................N............. .........N...................................................................... ..............i.......N.....................N................................... ......................................i......................................... ......N...........i............................................................. ................................................................................ ........................................................N....................... ..................N.........................................................N... Pos Score Pred ------------------------ 57 0.204 - 129 0.092 - 164 0.193 - 246 0.802 Yes 307 0.147 - 330 0.351 - 415 0.695 Yes 423 0.362 - 445 0.407 - 519 0.702 Yes 567 0.455 - 579 0.684 Yes 777 0.092 - 819 0.060 - 877 0.067 -

Explain the output -- Processing locus 1004: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGGCTTTGACCACATCGCCACTTATCGGTTTGCGGATGCTGGAAAAGTCAGTCTAGTGATGAGGCTGAGTGAGCCATCG TCAGTTACTATTTTATCGTGAAACTCTGAACAACGAGATTGTCCCGGATATTCAAATGAGGAACAAGGCCCAATAGTCCG ATCAAAGTGGGCTATTCGTATCGCTTACGAGGCCTATACGATGGTAATTGAATTCACGCGCATTCATCAGCGCGTGATTT GGAGTTGCTCAATTTCAGATCGCACAGGCTCTGTTAATCATCTCCTCCTCCGAAAGACAGAGAAAGAGACAAATCAAATT CATTCATATCAAAGAGATAGAGAGAAAATGTCCGGTGTGGCTATTCATGCGAGTTCATGGGGCGCGGCTCTGGTGAGAAT CTCCCCGTACACTTTCTCCGCCATCGGAATCGCCATCTCCATCGGCGTCTCTGTTCTTGGTGCTGCCTGGGGAATTTACA TTACCGGAAGTAGTTTGATCGGTGCCGCCATTGAAGCTCCTCGAATCACTTCCAAGAATCTCATCAGTGTAATCTTCTGT GAAGCCGTGGCTATATACGGTGTTATTGTGGCAATCATATTACAAACGAAGTTGGAGAGTGTACCATCATCAAAGATGTA TGATGCTGAGTCTCTAAGAGCTGGATATGCAATCTTTGCTTCTGGAATCATTGTTGGATTCGCAAACCTTGTATGCGGGT TATGTGTAGGAATCATTGGAAGCAGTTGCGCATTGTCTGATGCTCAGAACTCGACGCTCTTTGTTAAGATTCTTGTGATT GAGATCTTTGGGAGTGCTCTCGGGTTATTTGGAGTTATCGTTGGGATTATTATGTCAGCTCAAGCTACATGGCCTACAAA ATAGAGATATATAAACTGGTAACTTATGGACTTGTACTCTATAGTCTATACATTCTATGATCTATTTTTCAAAGTTCCGG ATAAAAATTACACTTCTTTGCTG ....................................N......................i.................... .......................................................N........................ ........................................i....................................... ................................................................................ ...........................i..................N.........N....................... ................................................................................ ................................................................................ ...........................................................................N...N ..N.......................N.............................................N....... ...................................................N................N........... .........................N..............................N....................... ....................... Pos Score Pred ------------------------ 37 0.097 - 60 0.569 Yes 136 0.288 - 201 0.533 Yes 348 0.903 Yes 367 0.269 - 377 0.462 - 636 0.330 - 640 0.239 - 643 0.409 - 667 0.381 - 713 0.474 - 722 0.532 Yes 760 0.068 - 852 0.227 - 869 0.481 - 906 0.205 - 937 0.038 -

Explain the output -- Processing locus 1005: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAGTACAACATGCTTAAGATCTAAACAATATTTTTATAATAGAAATAAAATAACTGAATAGGAGTATTATACTAATCG TGACGAGACGACAAATTGTAGACCATGTATTTAAATTTTGCCTCCGGGTTATGCGAAAAAAGGAAAAAGCATTCACATGT CTCTCTCCTGACATGCACGAACCAACTAATAACTTCTTATTATACTAATCACGTTATTATCTTATTAAAAAATAAACCCA TTTTGACCACAACCTTTCTCTATATAAACATTTTAGTCTCCTCCTCTCTCTTTATAACCACAACGATTTTCTCTCTCTCA CGATTTAAAAAGCTAAACAAAAACCATTGTTTTCTCTATCTTTCTTCTCCAAAAATGGCAGAAACAACAACAGTAACATC TTCATTGAAGCGTCAAAGAGATGAGCAAGAACAAGAAATCATCAAAACACAAGAAGAAACGAAAAGACAAAAGCCTTCTT CATCTTCTTCCTCGTACAACCAAGTTCTAACTCTCTTAAAAGATGAAGCCGAGAATGACATAACTTCCCTAATCACCACG CTTCAGCAAGAAATTTCTAATGAAGAGCAAAACGCTGCCGTTTTCGAAGAAAATCCTTCTCTCTCTTCTTCTTGCTCCTC TTCCTCTTCTTCATGCACTTCGAAAGAAGATGAGTACGACGGAGATAAAGAGAAAGTGATGAAGCATCTTCTTGAAGCAA CTGACGACGAACTAGGAATCCCTAACACTGAATTTGGCGGGAGTAATTATGAGATGATTAAGAGTGAGACTAGTCAGGAT TACGTTAATGGGTTTAGTTTATTAGATGGGTTTAGTGTTGGGCTTTGGGAGCTTGAGGATGAAGCAGCTAATTATTACAC GTTGCTACAATCAGAACTGTTCATGTAGAGAAGGGTAAAATAGGAAAACAAGAAAAAGAGGATGGGTGAAAAGTGAAAGT AAAAGACCAAAATATGATGATGAAGAAAAAAAGAGATTGTTTTCTTT ...........N.................................................................... ........................N.........................N.........................N... ............N................................................................... ................................................................................ ......................................................i......................... ....................N........................................................... ..........................................N...........N......................... ...................i............................................................ ............N................N............................i..................... ................................................N....i.......................... .......N.................N................................N..................... ......................N......................................N.................. .............N..N..N........................... Pos Score Pred ------------------------ 12 0.301 - 105 0.405 - 131 0.083 - 157 0.132 - 173 0.174 - 375 0.851 Yes 421 0.302 - 523 0.309 - 535 0.096 - 580 0.777 Yes 653 0.186 - 670 0.296 - 699 0.778 Yes 769 0.249 - 774 0.580 Yes 808 0.242 - 826 0.239 - 859 0.304 - 903 0.127 - 942 0.089 - 974 0.077 - 977 0.041 - 980 0.063 -

Explain the output -- Processing locus 1006: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGGCCTCTTCGTCAACGTAAATAATATCTTGCCTTATAATGATCTTTGACCAAAACAGGTCAATGGCTTCGTAATGATA TATATATCCAGTATCCACTAAGGTTTTCTTATTAACCTTTCAATGCACTATCAAATAAAAACAATGATGAAATTATTCTC TATTTTTGTCGTATTTATGCAAATCCAAGTAGCATTGTCGTCTCAACCAATTCGGTATGCTACGGAACCTAAGCCCATAC AAGAACTCTGCAAATTTAACATTAATCCAAGCCTCTGCGTCTCTACTCTCAATCTTGATCCTAGAAGCAAGAACTCAAAT TTACGAGAGCTTGCGTGGATCTCTATCGATGCGACTTCAAACAAAGTCAACAAAATGTTAAACTATCTCATCTCCGTCTC TAAGAACATCAAAGACCGTGAAGACTTAAAGAAGTACAAAACTTGCATTGATGACTATGGCACGGCGGCTCGTAGGTTTT TACCGGCGGCGTTGGATGATCTTAAAGCTGGGTTCTTTTCTCTAGCGAAGTCTGATATGGAAAGTGTTGTGTCGATACCC GATCATTGTGAGGCGCAATTCGGTGGGAGTTCACCGTTGACGGGACGTAACAAAGCCACTCATGATATCGCTAATATGAC TGCTGACATCATAAGATATCTTTTTGGCAATAATTAATAAGTTTTTAGTCTTGTTCAAAGTACAATCGAGTAACGCATAA TGAAGAATAAGATATTTATTTCCCATTTTTATTGACGAGAGTCTGAGTCTTTGATT N......................................N.......................N..........N..... ..........................................N....................i..i............. ................N.......................................N....................... ................................................................................ ............................N.........................i......................... ..................................................N.....N....................... ...............N........................................i....................... .............................................................N.............N.... ...............................................................................N ........................................................ Pos Score Pred ------------------------ 1 0.255 - 40 0.288 - 64 0.431 - 75 0.085 - 123 0.186 - 144 0.626 Yes 147 0.606 Yes 177 0.244 - 217 0.253 - 349 0.197 - 375 0.633 Yes 451 0.145 - 457 0.261 - 496 0.193 - 537 0.665 Yes 622 0.221 - 636 0.381 - 720 0.063 -

Explain the output -- Processing locus 1007: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGAAAGAAAAATATGAAATAAGACGACAATGTGTGATGTACGTAGAATCAGTTTTAGATTCTAGTACATCAATAGACA AGAAAAAGATTGTGGTTATGATTTCACCGACCCGAGTTAATGCAAATCCGAAACAGTATAAATATGTGTAGAGGGTTCAT GCCTATGATACAAATTAAAGAAGCAGAAACAAAAACAAGTAAAACAGAAACAATCAACACAGAGAAACCACCTGTTTGTT CAAGATCAAAGATGTCTATAAATATAAGAGACCCTCTTATAGTAAGCAGAGTTGTTGGAGACGTTCTTGATCCGTTTAAT AGATCAATCACTCTAAAGGTTACTTATGGCCAAAGAGAGGTGACTAATGGCTTGGATCTAAGGCCTTCTCAGGTTCAAAA CAAGCCAAGAGTTGAGATTGGTGGAGAAGACCTCAGGAACTTCTATACTTTGGTTATGGTGGATCCAGATGTTCCAAGTC CTAGCAACCCTCACCTCCGAGAATATCTCCATTGGTTGGTGACTGATATCCCTGCTACAACTGGAACAACCTTTGGCAAT GAGATTGTGTGTTACGAAAATCCAAGTCCCACTGCAGGAATTCATCGTGTCGTGTTTATATTGTTTCGACAGCTTGGCAG GCAAACAGTGTATGCACCAGGGTGGCGCCAGAACTTCAACACTCGCGAGTTTGCTGAGATCTACAATCTCGGCCTTCCCG TGGCCGCAGTTTTCTACAATTGTCAGAGGGAGAGTGGCTGCGGAGGAAGAAGACTTTAGATGGCTTCTTCCTTTATAACC AATTGATATTGCATACTCTGATGAGATTTATGCATCTATAGTATTTTAATTTAATAACCATTTTATGATACGAGTAAC ..............i...............i......i.......................................... .................N.....................N.......................N..............N. ....N........................................................................... ...........i.................................................................... .........................N....................N................................. .......................................................i............N........... ..............................................................................N. ................................................................................ ...........N.................................................................... ...........................................................N.................... ....................N........N..................................N............. Pos Score Pred ------------------------ 15 0.550 Yes 31 0.781 Yes 38 0.705 Yes 98 0.469 - 120 0.495 - 144 0.386 - 159 0.293 - 165 0.199 - 252 0.716 Yes 346 0.337 - 367 0.437 - 456 0.780 Yes 469 0.107 - 559 0.325 - 652 0.302 - 780 0.274 - 821 0.054 - 830 0.039 - 865 0.043 -

Explain the output -- Processing locus 1008: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCCCAAGGGAAGAACTCTAGTGATGATAGGGAGGTCAATTTACCTAGTAAAATTCTGAATAGATAGTTACTATTATGCAA TGTTAATAATAATCTGTTTGATTTTTTAACATTTGTTCGGACATCCAAACCTGTGGGACACAATTTATTACTTTAAAGAT AATATATCAAACAGAGAGTGCAATGGATAATATCAAACAGTTAAAAGGAAAAAAAAAAAAATTCTTTGAGATGAGACTTG TTCTCTCTGGTGCCTCTTCTTTCACCTCCAACCTTTTTTGTTCCTCCCAACAGGTCAATGGAAGAGGAAAAGAACTGAAA AACCCAATTTCTCTAAACCACAACAAAGATTTGGACTTTCTGTTAAAGAAGTTAGCTCCGCCACTCACGGCGGTTTTACT TGCTGTTTCACCCATCTGTTTCCCTCCAGAATCTCTTGGGCAGACTTTGGATATACAGAGAGGAGCTACATTGTTTAATA GAGCTTGTATCGGGTGTCATGATACAGGTGGAAACATTATTCAACCTGGTGCAACGCTTTTCACAAAAGACCTCGAAAGA AATGGAGTTGATACTGAGGAAGAAATATATCGTGTTACCTACTTTGGAAAGGGAAGAATGCCGGGATTTGGTGAGAAATG CACACCGAGAGGACAATGTACCTTTGGACCGCGGTTGCAGGACGAAGAAATCAAGCTTCTCGCTGAATTTGTAAAGTTTC AAGCTGACCAAGGCTGGCCAACTGTATCTACGGACTAGCGACTAGGCATTCTTCGTCTTGTGAGCGTTTTTGTAATCCAA TTAATCCATAGGGCTGTATCTACTTACACTTATAACAATTCCTTCTGGGAGGTTTCA ......................N...................................................N....N ................................................................................ ......................N...............................................N......... .........................................................N...................... ................................................................................ ................................................................................ ..................N............................................................. ...............N................................................................ ................................................................................ ......................................................... Pos Score Pred ------------------------ 23 0.216 - 75 0.237 - 80 0.273 - 183 0.315 - 231 0.493 - 298 0.148 - 499 0.387 - 562 0.770 Yes 618 0.711 Yes 638 0.550 Yes 656 0.184 -

Explain the output -- Processing locus 1009: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAATATATAATATAACCCATATAATTTGATGGGATGAGTACTAAAAATAGTTAGGATAGTTTTTTAGTAGGAAGAGTAC TAAATTTGGATCATTGGGTAACTTATGGGTCTTTCTAACTGGACCAACAATGATATCCACAATAGGCCCAATATAATAGA GCGTGTGATAATACATCCCAAACAAATCTCAGGCTCTAACCAATTACGAACCTGTGAATGTCTTACACGTGGCAAGTCAA ACTACAGAGTCTTACAAACTTTGAATTCCCAATTTACCCTCAACGTTTCTGTTATATTATGCTTCTCCTTTCTCTCAGTG TCAGTCATAGGGTCACCAATGGCTTCTTCTTTGCTTGCACTCTTCTCCTGTCTCTTCCTCTCTCTCTTATCTCTCTCCTC CTCCCTAAATCTCCGCCGTCCGATCTTCTCTCAATCCAACGACCTCGATCTCTTCTCTTCTCTAAATCTCGACCGTCCAT CTCTCGCCGCCGATGACATCCACGATCTTCTCCCACGCTACGGATTCCCGAAAGGTCTTCTTCCCAACAACGTCAAATCG TACACTATCTCCGACGACGGCGATTTCACCGTTGACCTGATTTCCAGTTGCTACGTCAAGTTCTCCGATCAACTCGTTTT CTACGGCAAGAATATCGCCGGAAAACTCAGTTACGGATCTGTTAAAGACGTCCGTGGAATCCAAGCTAAAGAAGCTTTCC TTTGGCTACCAATCACCGCCATGGAATCGGATCCAAGCTCTGCCACGGTTGTGTTCTCCGTCGGATTTGTGTCCAAGACT TTACCTGCTTCCATGTTCGAAAATGTTCCTTCTTGCTCAAGAAACCTAAATCTTCAAGACTCTTGAATCCACCTGAAACG ATCTCAAGATTCAACATTCCCTCCACCCTTTATAGTTTTGTATTTCAGAAGTATTTTGCTTGGTTTCGTAGATATAGGTT CGAAT .............................N....i............................................. ........................N........................i.............................. .........................................................N...................... ..........................................................N..................... ..................i............................................................. ................................................................................ ............N................................................................... ................................................................................ ................................................................................ ....................i........................................................... ............N.........N......................................................... ................................................................................ ..... Pos Score Pred ------------------------ 30 0.466 - 35 0.544 Yes 105 0.239 - 130 0.514 Yes 218 0.129 - 299 0.267 - 339 0.744 Yes 493 0.372 - 741 0.714 Yes 813 0.193 - 823 0.257 -

Explain the output -- Processing locus 1010: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCATTTCATTCATTTTTATATTCTTTCGTTATAATTATTATACATCCTTTGAAAGAAAAAGAAGAGGCAATAGAGATGG ACGGCTAAGATGTTAACGTAAACAAAGTTGGCGGAGGGAAAAGAGGAGCGTGGAATAATTAAACAAAAGAGTCGTGTCCA CATGGGCGGCCTCAAGCTTCGTGTCCCACTTTTGTCCCCTTGCCTCTTTCTTGCCTTTCATGCTTCCGACAACACTTCTC TCTCTTCCTTTAACCCTAACTTCGATGCTCCTTTATATATATCTCTCTCCCCTTTTCTCCAAAACTCATCAACCAGCTCA CCAAGAACAAGAACAAGAAGAAGAAGAACAAGACAAGAAGAACAAAGTACCCAAGAAAACACACAAGAAGGAAACACAAA GATTGATATTGATCCAAAAGCAATGGCGTACGAGAAAGTCAACGAGCTTAACCTTAAGGACACAGAGCTATGTCTTGGAT TACCCGGAAGAACAGAGAAGATCAAAGAAGAACAAGAGGTTTCTTGCGTTAAAAGTAACAACAAGCGTCTATTTGAGGAA ACTCGTGATGAAGAAGAATCTACACCTCCTACCAAAACTCAAATCGTTGGTTGGCCACCAGTGAGATCTTCCCGTAAGAA CAACAACAGTGTGAGCTACGTGAAAGTGAGTATGGACGGAGCTCCTTACCTTCGCAAGATCGATCTCAAGACATACAAAA ACTACCCCGAGCTTCTCAAAGCGTTAGAGAATATGTTCAAAGTCATGATTGGTGAATATTGTGAGAGAGAAGGATACAAA GGATCTGGATTTGTACCAACATACGAAGATAAAGATGGTGACTGGATGTTGGTTGGTGATGTTCCATGGGACATGTTCTC TTCTTCTTGTAAGAGACTCAGAATCATGAAGGGATCCGACGCTCCTGCTCTAGACTCTTCCTTATGATCCAGAGAAGCTG AGAATCTTTTGTCTAAAAAGGAGATGATGACTATGATCGATGAATGAAAAACCCTTTTTGGTTTGATTCGGATCGGAGTT TGACTA ............................................................................i... .........N...................................................................... ........................i....................................................... ................................................................................ ......................i..............................................N.......... ................................................................................ .......N........................................................................ ...............................i................................................ ................................i...........i................................... ..................................i..........N............N......N......N....... .........................N.....................................N................ .......................N..N.....N......N...N.................................... ...... Pos Score Pred ------------------------ 77 0.775 Yes 90 0.354 - 162 0.458 - 220 0.344 - 265 0.588 Yes 423 0.800 Yes 470 0.417 - 568 0.328 - 672 0.697 Yes 753 0.669 Yes 765 0.561 Yes 835 0.600 Yes 846 0.401 - 859 0.201 - 866 0.115 - 873 0.297 - 906 0.497 - 944 0.229 - 984 0.053 - 987 0.068 - 993 0.077 - 1000 0.108 - 1004 0.093 -

Explain the output -- Processing locus 1011: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTTTACCGATTAACTAGTACTGACAAAAGAAACAAAGAGTAATTATATTTTTTTGATAAATTGTTTTATTAGAAAAAT ACTTAAAATTGATAAAAATAAATAAACCCAATTGATATATTTGATATTACAATACCAGATATTGAAACAAATAAGACATG TTTTGTCTATATACTTCCAACTAGAAGATATTTATTTATCCATATCCTAGATATTTTAATGTGTGAATCTAATACATTCT CTTATATACGACCAAATAGTAAAACAATATGCCTCTCCTGCAATTTTTCTTCATCCAATATTTCTATGGAGTCATCTTCT TCACTCCTTCATCACTCTTACCTCTCTTACCTTAATCCAAAATTTGGAAAAAGACCTTTGGTTTCTTATCCATTAATGCA GAGTTCGCGAAAATGCAAACAAACTCGCATTTGTTCTAACAAGATGTATGTTCCCGGCTTTGGAGAAGCTTCACCGGAGG CTAAGGCAGCGAAGCATCTTCATGACTTCTTTACTTACGTTGCAGTGAGGATAGTGTCTGCTCAGCTTGAGAGTTATAAT CCTGAGGCTTATATGGAGTTGAGAGAATTTTTAGATACAAACTCTGTAAGTGACGGTGATAAATTCTGCGCCACTCTCAT GCGTCGCTCTTCACGTCACATGAACTTAGCCCTTCGAATTTTAGAGGTACGGTCTGCTTATTGTAAAAACGATTTCGAAT GGGATAATATGAAGCGCCTCGCCTTCAAGAACGTAGATGATTCCAACACAAGACTCATGCGCGAGTACGTCTTGGAGACT AGCCATGTCGAAACCGATTCTGATAAGTGAAACCGAATATCTCTTCGAATCACATTCATATATATAGAGTCTTGAGAGTA TACACACTTGAAACCGTTTGTACATACATATACATACCATTGTGCTATG ................................................................................ .............................................................................N.. ..........................................................N..................... ............................N....................................N.............. ...........................................................................i.... ............i..............................i...N................................ .....................N.......................................................... ............i.................................................................N. ...................i..........................................................N. ........i...........................N...................N....................... ....N........................................................................... ..............................................N.. Pos Score Pred ------------------------ 158 0.468 - 219 0.357 - 269 0.292 - 306 0.384 - 396 0.696 Yes 413 0.530 Yes 444 0.724 Yes 448 0.155 - 502 0.195 - 573 0.642 Yes 639 0.399 - 660 0.566 Yes 719 0.348 - 729 0.744 Yes 757 0.382 - 777 0.420 - 805 0.364 - 927 0.060 -

Explain the output -- Processing locus 1012: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACTAAGATCACAAAATCCGCATAAGATCAGATCTTGCTGTCTCATCTTCATCATCAGAAGTAGAGAATCTTTTAAAGGAA TATTTACTTTTATTAATTTAAGACCACCAGATGGATGATTGTCGGGACAAGAGAAGAAGACGGTGTACGAAGCTGACATG TGGTACTGATAATAACGACATGGAGAAGATGATGCACAGAGAAACTGAGAGGCAAAGGAGACAAGAAATGGCTTCTCTTT ATGCCTCTCTTCGTTCTCTTCTCCCTCTTCACTTCATCAAGGGTAAGCGTTCGACGTCAGATCAAGTCAACGAGGCGGTG AACTACATAAAGTATCTACAGAGGAAGATCAAGGAGCTAAGTGTGAGGAGAGATGATCTCATGGTACTGTCTAGAGGAAG TTTATTGGGTAGTAGTAATGGTGATTTTAAGGAAGATGTGGAGATGATAAGTGGGAAAAATCATGTGGTGGTTCGTCAGT GTTTGGTTGGTGTGGAAATCATGTTGAGTAGCCGCTGCTGCGGTGGCCAACCGCGGTTTTCGAGTGTTCTTCAAGTGCTT AGTGAATATGGTCTCTGCCTTCTTAACTCCATCTCCTCTATTGTTGATGATAGGCTAGTTTACACCATACAGGCCGAGGT CAACGATATGGCTTTGATGATTGACTTAGCAGAACTTGAAAAGAGATTAATCAGAATGAAGTAAATTCTTGTTGAGTAGG TTGCTCTTAACTTGTCAAGTAAACGACATATATAGTTAAGTACGTACTTTATTCGTACCTGTGATTGTTGTGTCGTGTTC ACA ................................................................................ ..............................i...N..........................................N.. ...................i........N..i...................................i............ N............................................................................... ....................................................N.......N................... .................N.................N.......i..................N................. ....................N........................................................... .......N......................................N................................. .......i........N......................................N........................ ................................................................................ ... Pos Score Pred ------------------------ 111 0.623 Yes 115 0.387 - 158 0.299 - 180 0.502 Yes 189 0.456 - 192 0.520 Yes 228 0.750 Yes 241 0.247 - 373 0.186 - 381 0.254 - 418 0.251 - 436 0.378 - 444 0.594 Yes 463 0.271 - 501 0.345 - 568 0.296 - 607 0.202 - 648 0.592 Yes 657 0.189 - 696 0.198 -

Explain the output -- Processing locus 1013: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCCTTGAAATACACTAATTCTCACACGCAATTTTGGTAACTTACAAAGACTTTTTTTGACTCTTGACTGATTCATTCA AATGCCTAATTTCATATATAAAATAACAAATTTCTTTCTCAAATGACCACATACTCAATTTACAAAAAAAAAAAAAAAAG AAGAAAGATGAAAATGCATCACATTCTCTCGATAGTTTTGGCAATTATACTCGCTTCCTCCGTTGCAGAAGCTTTTCTCG ATGAAAAAACTCAACAAAGGGTCAACGGAATCTGCAAGCAGACAATGGATACCAGATTTTGCAGTAGTCTCTTGATCAAG AACCTGAATACTTTTCCCGCAAGTAACAAAGAAATCATGAACGTGACAGTAAGCGAAGCTGAAAGGTTCGCGGCCAACAC TTATTTCTTCATCAGCACGCTTCTCAGGAATGCTGGAGACGAGAGGCCTGATCTTCAAGCGTGCGCCGAAGCTTACGCCA TTGTGAACTCGGCGTTCACGAAAGCTGTAACCTTCTTCAAGCAAGCTTACTACAGTAAGATTGTCAATATCGAAAAGAAA GTTTCTATGGCTGTTGATATCTGTAAGACAGATTTCAATGTGCTCGGTTATCAGATCAATCCGTTGATTGAGAAAAACAG ACAGACGAAGATCTTACTATCCATGGAACAGATTGTTAGTCATATGGTTTCTTCATAATCTGATTGTTGTTGAATCATGA CATAGTAGCAAATGTTTCCAGAAATATTCTAATTAATAATGGTTACAGAATCTTCTTAATTGCAATTCTCTATATGC ................................................................................ .......i.....i.................................................................. N...........................................i................................... ....................................i........................................... .............................N.................................................. ................................................................................ ......i..............................N.......................................... ......................N....................N................................N... ...........N..........................N..................................N... Pos Score Pred ------------------------ 82 0.385 - 123 0.287 - 168 0.804 Yes 174 0.800 Yes 241 0.097 - 285 0.739 Yes 357 0.699 Yes 430 0.142 - 567 0.709 Yes 598 0.207 - 663 0.172 - 684 0.196 - 717 0.036 - 732 0.076 - 759 0.124 - 794 0.040 -

Explain the output -- Processing locus 1014: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTATAAAAAAAGACTTATTCTCTTTCTCTTTTTGTACATAAAGAGGAAGCAAACAAAGAGAGCCAAAATGGTCGACATCT TCTAACAATTATTTATACTAAATTATATATTCACACATATCATGCTTATATATATATACACATGTATATAATGGGAAGGC AGAGGTAATGATGATGATGATAATGGGGAGAAAATGTGAAGATTGTGGGAATCAAGCGAAGAAAGATTGTGTGTACATGA GATGCAGAACTTGCTGCAAATCCAAAGCCTTCCATTGCCAAACTCACATCAAGAGCACTTGGGTTCCTGCCTATAGAAGA TCTCATCACAAACACCAATCGCAACCGCTCTCTACTAGTATCCCAAAAGGTGTACAAATCCACACTACTCCTGGACATTT TCCGGCAGAGTTGAGTTCCCTAGCAGATTTCCGATGCGTAAAAGTGAGTTCAATCGATGATGGAAAAGAGCAATATGCTT ATCAGACCACGGTGAACATTGGAGGACATGTTTTCAGAGGGATTCTTCACGATCAAGGACTCCACAAAGTGATGGTAGAT CATCATTATAATAAAAATAGTAATAATCATCAAGAGTTACTTACTCCTTCAACTTCTTCATGTCCCTTGAAGATCACAAG TCCTTTTACCGATTTCATGTTCGGTACCCGATTTTCTTCGGTCCTAAGAAGATAGATAAATTTTAATGGATGGAATGATG CCTAGCTATTTCAAGTTAGGGCCCATCGTATGTTGTTACTTTACTTGCTCCCATATCTTACAATTTTTTTTTAT ...................................................................N............ .........................................N...................N........i......... .......N..N..N..N.....N..........N..........................................N... ................................................................................ .................................N......................N..N..............N..... ...........................N...........................................N........ ...........................................................N.................... ................N................................................N...N....N..N.. .............................N............................................ Pos Score Pred ------------------------ 68 0.448 - 122 0.433 - 142 0.378 - 151 0.620 Yes 168 0.458 - 171 0.385 - 174 0.404 - 177 0.398 - 183 0.210 - 194 0.375 - 237 0.317 - 242 0.313 - 434 0.459 - 457 0.485 - 460 0.337 - 475 0.200 - 508 0.169 - 552 0.482 - 620 0.281 - 657 0.358 - 706 0.321 - 710 0.142 - 715 0.073 - 718 0.141 - 750 0.047 -

Explain the output -- Processing locus 1015: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAAGATAAATGCAATAAAACAATGAATCGTAAGATGAATTACTAATATCAAATTGGATAGGCATATTCCAATCTGAGAC TTCAGAGTTAGAGAACTTTGAATCTCTACATGTATATATATTAATGTTATGCTATTTTCGTTATGTAACCAAACAAAAGA GCTTTTGAGTTTTGGTCGTACAAAAAAAAAGAAAGGGAAAATGAAGATAGGACCTGTAGGGAAGCATGATGCTAGAAGCA CTACAATTGTAAACTGGGACGAAGGATCTCACGATGGCTTCATCTCTCAGATTTTTCTATCTCATGGCGTCGCTGGAATT ATGTCTATCCAATTCCAGTTCGTGATGGACGGAAAACTCGTTTTATCCGATCGTCACGGTCCATTTTCCGGCAACATGTT TGACGTTATAGAGCTCAACTATCCGCATGAGTACATAACTGGAATCAGTGGAGAGTATTACAAGTATGAAGCCAACAATC CTCACATGAGGTCTCTCAAATTCAATACCAACACTAGCGAGTACGGTCCTTTTGGCACCTCCGGTTCGAGCAACGACAAA TTTGCTTTTAAACTCGGGAAATCTCCTCAGTTCGGCGGTTTCCATGGGACTTATGATGCCTCGGGACTACAGTACATTGG TGTTTATCTCCGCCCAAAAACAGTTCTACCCAAAATAGATACGGGCAATGCAGAGGAAACAGAGTCAAAAATTGTACTGG GCTGAAAGTGTTTGTATACGACTCTATTCTGCTGATTGTACATTCTTAATTATGAAAATTCTTCTATGAATAAACTGATA TGTGTAACATATAATCGTCAAAAT .........N............N...........N............................................. .............................i.............N....N.............i................. ........................................i........................N..N........... .................................N.............................N................ N.......................N..................................................i.... ..........................N......................................N.............. .....i.......................................................................... ...........................................N........N..N........................ ...............................................N................................ ...................................................N.............N.............N ........................ Pos Score Pred ------------------------ 10 0.270 - 23 0.488 - 35 0.205 - 110 0.629 Yes 124 0.200 - 129 0.250 - 143 0.672 Yes 201 0.549 Yes 226 0.112 - 229 0.130 - 274 0.361 - 304 0.213 - 321 0.394 - 345 0.415 - 396 0.735 Yes 427 0.498 - 466 0.384 - 486 0.659 Yes 604 0.206 - 613 0.200 - 616 0.461 - 688 0.242 - 772 0.068 - 786 0.072 - 800 0.055 -

Explain the output -- Processing locus 1016: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATTAGTTTTTAATTATTTATTTTTTGATTGAAAGTGTTCCGAATTACATAAGAGGTATAATTTGTCGGAGAAATTAGGG AATGAGACAAGAAGTTGCTAGAATCCTCTGGCGTTTTCATCACTACTAGTCTCTCCTTGTCTTTTTTTTTCCTTGGATTC TCTCCATTCGCAGATGCATTACGCCATGAACAGTGATCCCGAGACGCAGATGATGGATGCCGACTCCACCGAAACGTTCA TGCTCGACAAACCCAGCACCGCCAGAGATTTCCTCTCCGCCGCTCGTAGGCTCGTCGATCAAGGAGAACCATCTCAAGCT CTCCAAGCGGTCGTGATGGCGATGAGAAACCAAGGAGGAGATGAGGCGGTTCTTCAGATACTGAACCGTACCCGTGAACT ATACAAGCGTAGAATTCAAGAAACAGCTAGTATGGATCAGCTGGCTTCTATATTTGCAGAGTGTGCCATTACAGAAGCAC AGCCTCTTGCGCTTGATGAGCCTACAACGTCAAAAGACTTATTTGGTACCAAACAAACAGTTACAGCAGATGCTCATGGC ATATCTATTCTGGAAAAAAGTGGGAGGTCACAGATCATGCTCGATGCCTTTGCTGACGGAAGCAGTTTTATCTGTCTGCA ATGCGGTGGTCTGGTGAGCATCCATAGGAGAGATGAACACTATGCGTACTGGTGTAGCAATATGTAATACAAGGATGCTA TTTTGTTGGCCATTACATGAGAATCTGCGGCAAATTGCCTTCACTGTAATATTTTGTTGCTAAATGTTGTTGATCTTTGA ATGGAA ................................................................................ .............i...........i.......................i..i...i......................i ................................................................................ ...............i.....N..................N....................................... ...............................i................................................ ...............N.....................................................N.....N.... ....................................i......N.................................... N...............................N........N...................N............N..... ................N..............................................N................ N..... Pos Score Pred ------------------------ 82 0.214 - 174 0.631 Yes 186 0.760 Yes 210 0.846 Yes 213 0.774 Yes 217 0.654 Yes 240 0.587 Yes 336 0.675 Yes 342 0.425 - 361 0.221 - 432 0.844 Yes 496 0.269 - 550 0.383 - 556 0.355 - 597 0.629 Yes 604 0.299 - 641 0.441 - 673 0.196 - 682 0.247 - 702 0.377 - 715 0.067 - 737 0.054 - 784 0.035 - 801 0.043 -

Explain the output -- Processing locus 1017: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAAATGATTGAAGACGTACAGTGTTGAGTAAGTAGCCACTGGTTTGAAGAGAGAGGCAACGAAAGCGACTGGTAATAGC TGTAGTGGCACTCTGTTGTAATTAATCCAATTGGTACTACCCTTATTTGTAAACATACACGAGTCCTTTAAAATCTTATC TATAGACAAAGTCACGGTCCTCATGCACTACAATATCAGAATGCTTATCACGCCGGCGAATTTATCCGGTGGCATAGCCG GATTATTCGTTTTCGGACAGAAAGATCAAATGACTAAAATACCCTCGCTCACAAACTCGTATAACTACCGGAAAAAACTT TTCACCGTCCCTCCAGTGGCGGAGAGAAGAAGAGCTTTGAAGATGTTCTGGTGCGGTGCTCGGAGAAGGGTGAGAGACGA GGGAGGTGGTGAAGATGAAGATGAAATGTATGGATACAACGAGGAAATGGCGATGTTGGAGATATATAGCCAGTCGTGTA GAGAAGAAGCGCTTATAGTGACGGCCATTGTCGACGATGAAGAAGTGGAAGTTGTAATCTTTAAAGGAGTGTCGTCGTGC TTGAGCGGAGAAACGGCGGTGGATCCGGCGAGGAGTGTGTTGCCGGAGAGAGCAGTCATTACAAAGATTGATAGAGTGAG AGGTCCTTTTGATCCTTCACAGATTCATTATATTCAAAGGGACATCTCTTTTCAAGCCTTCAAGGAGACCAGACTCAACT AATGTTAGTGAATGTTGTGTCTGGATCAATGAAACTAATGACAATTTCAATTTTTTCACTAATAACTCTCACCTCTGTTT CTGAACTAATTTCTTAAATCT ....N........................................................................... ................................................................................ ......................i.................N....................................... .............................i.................................................. ..........................................i..................................... ..............N.....N....i...N................i.....i........................... ....................................N........................................... ................................................................................ ................................................................................ ..................... Pos Score Pred ------------------------ 5 0.317 - 183 0.591 Yes 201 0.454 - 270 0.639 Yes 363 0.696 Yes 415 0.230 - 421 0.256 - 426 0.732 Yes 430 0.087 - 447 0.671 Yes 453 0.662 Yes 517 0.110 - 722 0.374 - 732 0.076 - 749 0.069 - 758 0.050 -

Explain the output -- Processing locus 1018: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAATATCTGGAGAATTAGGGATTTTGTTCTTCGATATCTAATTAGATCATTCTTGATTGATTAATTCTATTCTCTGTTAA AGTTTTTTTTTTTAATCTTGATGTGTATTTGTTGAACAGCATAAGGACTTGAAGCGTTTATTTCTATCAGCGTTCTACCA TGTTAACGAGCCTCACTTAGTCTATAACATGATGTCACTTCTCTGGAAAGGTATCAAGCTTGAGACATCAATGGGAAGCA GTGAGTTTGCTTCAATGGTCTTTACTCTTATTGGTATGTCTCAAGGAGTCACATTGTTGTTAGCCAAATCGCTTCTTCTC TTGTTTGACTATGACAGAGCTTACTACAACGAGTATGCGGTTGGGTTCTCGGGTGTTCTCTTTGCTATGAAAGTTGTTCT TAATTCTCAAGCTGAGGATTACTCAAGTGTGTATGGTATCTTGGTTCCAACAAAGTATGCTGCTTGGGCAGAATTGATTC TAGTGCAGATGTTTGTACCTAACGCTTCGTTTCTTGGGCATTTGGGTGGAATCCTTGCTGGTATTATCTATTTGAAGCTG AAAGGTTCTTACTCAGGCTCAGATCCAGTCACTATGGCGGTTAGAGGTGTATCTCGATTGGTGACGTGGCCCTTGAGGTT TCTGAATGGTATGGTTAGGTCTCGGAGAAGGAGGATTACGGGGAGAGGAAGAGTGGGAAGAGGTCAGACTGGGATAGCAG GACCTGGTATTTGGAGATGTCAGTCGTGTACATATGACAATTCTGGTTGGTTAAGTGCTTGCGAGATGTGTGGCTCGGGA CGGGCTAGAGGAAATGGATGGTCACTGAATCAGGGACCAGCACTTTCTTCTTCTAATGATCTCCCTTTAGATGAGCTGCG CCGTCGAAGAGTAGAGAGGTTTAGTTGATCTCGGGAGCGCATTCTTAAAGCTGAGGAAGGATTGTGAATGAAGCTTTGTT GAATCATTCATTGCATTAGCTTATGAATAGCAGAGTTAGTGGTCGTG ................................................................................ ....................N..........................................................N ............................N..i......................................N......... ..............N....................N............................................ ..........N.......................N...............................N............. ................................N.......................N....................... ........N....................................................................... .................................i.............................................. .....N....N..................................................................... ................N................N...............................N.............. .............i...N.....................................N..............N......... ...................................................................N............ ......................N........................ Pos Score Pred ------------------------ 101 0.245 - 160 0.197 - 189 0.336 - 192 0.553 Yes 231 0.247 - 255 0.448 - 276 0.135 - 331 0.176 - 355 0.310 - 387 0.487 - 433 0.091 - 457 0.122 - 489 0.319 - 594 0.619 Yes 646 0.405 - 651 0.220 - 737 0.389 - 754 0.286 - 786 0.450 - 814 0.615 Yes 818 0.292 - 856 0.418 - 871 0.160 - 948 0.062 - 983 0.040 -

Explain the output -- Processing locus 1019: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACATCGTTCAAAATAGTAATCACAACCTTTTATATGCTTGACACGTGGCGGGACAAGGGACACTTGAACTTCTACTCAGT CGCAGGAACTGGACGGAGTAGGAGAGACAAAGAAGGATGAGTATGTTGTCAGGGAAAGCTGCTTACGATCTCTTTGTCTA TGGCAGTCTCCAGGAACCCGAAGTCGTATACGTGCTTCTTAATCGCGTCCCTGACCATGTCTCCGCCGTACTCTCCGGCT TTCACAGGTTTAGACTCAAAGGCCGTGTCTATCCAACAATTCTACCGGACGGTACCGGAAAAGTCAACGGAAAGGTGCTA AAGGGCATAACAGATGATGAACTGAAGATGTTAGATGAGTTTGAAGATGTTGAATATGACAGAAAGACTGTTGAAGTAAT GCTGACGGATACTTCAGAGAAGCTGCAAGTCGAAACATATGTATGGAAGAACAAAGATGATCCGGATCTTTATGGAGAAT GGGATTTCGAGGAATGGAGACAACATGACAAGGAAGATTTCGTAACAGCGACCAAGAAGTTTCTGGAAAATAGGAGATTA CCAGAAGCAAAGACGAGGATGGACACATTCAAAACATTCTTTAAGCAGGATCTTGAGAATGGGAAACCTCTGGACTCTTG ATGATACTTGCACTTATACTAGAGTGACTGCTATTGCTTCACTTGAAATAAGACTTGTGGAACAATGCTTCTTGGCATGT TTTGAAGAAATCGAATGTTC .................................N.............................................. ....................................i.....i....................................N ........................................................i....................... ................................................................................ .............N..N..........N......N...........N........N......................N. ......................................N...N.............N..............N......i. .............i..........N....................................................... ..................N.......................................i..................... N...............................................................N...........N... ..............N..... Pos Score Pred ------------------------ 34 0.356 - 117 0.716 Yes 123 0.573 Yes 160 0.406 - 217 0.609 Yes 334 0.237 - 337 0.260 - 348 0.481 - 355 0.255 - 367 0.147 - 376 0.309 - 399 0.235 - 439 0.379 - 443 0.385 - 457 0.373 - 472 0.130 - 479 0.609 Yes 494 0.657 Yes 505 0.460 - 579 0.233 - 619 0.639 Yes 641 0.263 - 705 0.050 - 717 0.044 - 735 0.039 -

Explain the output -- Processing locus 1020: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCTAAATTCTAATATTAGGGACACAAAAGAATTAGAGGCTATAATTAAAAGAATTAACAAACTCACTAATTTACTAAT GAACGATTGTTCTCTATTTAGCTAAAAATAACAGATGGTTTTAACTTTATGATTTTGTATAAAACCGAACTTTGTGGCTC TTGTGAATTAGTTGTCCTCTTCTCTAACATCTCAAACAAAGAAAACGATTACAAAAGAGAAGAGGTATCAAAAAGAAGAC CAGAATCTTTTAACATTTTGAAACAAATGGCGTCCAAAGCCATCTTCTTCTTCTTTGTCTCTGCCGTGTGCCTGTCCTCT CTCGCCGGTGTGGCCATCGCTGATGCCGATGACTTTGACCGTTTCCAAATTCAGGGATCGGTTTATTGTGACACATGCCG TGTCCAATTCGTTACCCGTCTCAGCAAGTTCCTTGAAGGCGCGAAAGTGAAGCTAGAATGCAGGAGCAGAACAAACGGAA CCGTAACATTGACAAAAGAAGCCGTAACGGACAAAACAGGGAGCTACAGAATGGAAGTGACCGGTGACCATGAGGAGGAA GTTTGCGAGCTTGTGCTCGTCGAATCACCAGACAGTGGTTGCAGTGACGTTAGCAAAGAGGCTTACCTACGTAACGCCGC TAAGATCAGCCTGACCGCGAATGACGGAATCGTCTCTCACGAGACACGTATCGTTAACCCTCTCGGTTTCATGGTTCAAA CTCCGTCAGCTGAATGTCCCGCTGCTTTCAAGGAGCTCGGGATCGTTCCTGATGTCACCTTCTAAATTTAATGATTTCAA GATCGTCATTTAAGTGTAATCTATTGTTTGTTTCTATATAACAAAACAAAAATGTAATTTGATAAAACACAAAAAAAATG CAGT ..............................................................................N. ..................................i.............N............................... ................................................................................ ..........................i..................................................... ......................i.....N.............................................i..... .........................................................i...................... ..................................................i..................i.......... ................................................................................ ....................N.................................................N......... .............N.....................................N..................N......... ...................................................N.........................N.. .... Pos Score Pred ------------------------ 79 0.148 - 115 0.694 Yes 129 0.414 - 267 0.781 Yes 343 0.552 Yes 349 0.250 - 395 0.701 Yes 458 0.697 Yes 531 0.644 Yes 550 0.659 Yes 661 0.122 - 711 0.353 - 734 0.200 - 772 0.353 - 791 0.145 - 852 0.081 - 878 0.056 -

Explain the output -- Processing locus 1021: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCGTTGGTTAGTAACTAAGTAATATAAATACCACAAGAACAAGAAATACTAATGAAAACTTGGAATGTTCAAAGTCTCCT TATCTACTTATGCAACGTTAGACAAAGCTATCAAACAATGCCACGTCACCTCACAAAACATCTAGGGTAACCAATCACAT GCAGATAACTCTCTCGCTTTATTTTCTCTTCAAATCTTATCAAAAATCCCATAATGTTTTCATTTCTTTACAAGTGATCA ACAAAACACCCCAAAAAGTAAAGAAAATGGCCGCAATTACATCAGCTACCGTCACCATCCCTTCTTTCACCGGCCTAAAG CTCGCCGTCAGCTCAAAACCTAAGACATTATCCACCATCAGTAGATCCACTTCCGCCACCAGGGCGCCACCTAAGCTCGC TTTGAAGTCCTCTTTGAAGGATTTCGGTGTCATCGCAGTGGCAACAGCAGCTTCGATCGTTTTAGCTGGAAATGCGATGG CCATGGAGGTTCTTTTAGGATCCGACGACGGTTCCCTTGCTTTCGTACCATCTGAATTCACGGTGGCTAAAGGAGAGAAG ATCGTGTTCAAGAACAACGCAGGGTTCCCACATAACGTGGTGTTCGACGAAGACGAGATCCCTAGTGGTGTGGACGCAAG CAAGATCTCGATGGATGAGACAGCGCTTCTAAATGGTGCAGGGGAGACTTACGAGGTTACTTTGACAGAGCCAGGCTCTT ACGGTTTCTATTGTGCGCCGCACCAGGGTGCTGGTATGGTTGGGAAACTCACCGTCAAGTAAATTGTCTAGATATTCTCT TGTTCGTGTAGGAGCTTACATATTCATGATTTATAAATCTAATTTGGAAGAGAATGTTGGATTGATAAAGAGGATCATAT ...................................................N............i............... .........N...........................N........................................N. .....................................................i.......................... ..........................i..................................................... ................................................................................ .......................................................................N....i... ..i............................................................................. ................................................................................ ..........i...N.................N............................................... ...................................N............................................ .........................N...........................N.......................... Pos Score Pred ------------------------ 52 0.415 - 65 0.752 Yes 90 0.215 - 118 0.493 - 159 0.464 - 214 0.509 Yes 267 0.835 Yes 472 0.312 - 477 0.733 Yes 483 0.631 Yes 651 0.746 Yes 655 0.288 - 673 0.309 - 756 0.222 - 826 0.084 - 854 0.053 -

Explain the output -- Processing locus 1022: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTGTTCGCAGAGTCTTTGATTAATTTTTTGCATCTGCTGCTAATTAGGTTTTCGATCTAAAGGGTTATACGCTTGGACT ACAAGCTAGAACCTGTAATAAATGGTCTCCTTGTCCTGTCTTAGTCTTACTCTATGCTACTCTGGGCCTTCATCGTTACA ATTTGCTTCAGGTCAGAAAATATATATGATTCATGTTCAATTGACAATAATGAATCACACATATTTTGACATTCAGTTTT CGTTTCTTCAGGGTACAAAGTTTCAGCTCAATCGCGTAAAGAAGTACGTTTTCTCAACGGGTGCTGCTGCCTCTTACTAC ATAACTTTGGATGCAATGGAACCAGCTGCTGACTCCTCCTCTTCGCTTCAAACCTTTCAGACTGAAGTAACAGAAGATAG TTTTGGCAAATTTATTCTGACGTGCAATATTGCAAGAATTCGAGGGGAACCAAGCAATGTCTATACCGGGTTGAATATAG GTTGTCGCTTGCCGGAGTGTCCGCCAGAGAATCCTTTTGAAATATATTATCTGATGAAGGATTCGGAGCTCCAAGAAAAT AATGACTGGATTCGTCTATATTTGGAACTTGCTGTTGCCACCACTGATAGGAACAGGGAAGCTAGTGATTTTGGTCTCTC CAACTTGGAGATTGTCAACGTGGCCGTTGATGCGGAGGGACTCCTCAATGCCAAAAATGCAATTTTCTACATTAGGTACA AGGACTTGTACGAGGCTCGGCTTGGTAATGCTGTTTTTGATCGCATTGCTATCGTTAGAAGAATCTTCGATGAGGATACG GGACGCTTCACTCTCGTGGGTCAGAATCAGAGTTCAGATATTATAAATTAACTATTTATTAATGTTAGACTTTAAGTTAT TTGTATAATATTGAGTGTAGATTTGGTTTGTAACAGTATTTTGCTTGAATAACTTATCATAACATTATGT ................................................................................ .....................N...............................N.......................... .........................N......N................i.............................. ................................................................................ ..........N....i................................................................ ........................................................i....................... .....................................................i.......................... .............................N.................N........N....................... ...........................N.........................................N.......... .............................................................N.................. ..................................................................N... Pos Score Pred ------------------------ 102 0.235 - 134 0.243 - 186 0.347 - 193 0.140 - 210 0.550 Yes 331 0.109 - 336 0.747 Yes 457 0.597 Yes 534 0.530 Yes 562 0.441 - 670 0.184 - 688 0.079 - 697 0.291 - 748 0.141 - 790 0.106 - 862 0.061 - 947 0.054 -

Explain the output -- Processing locus 1023: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAAAATCTATTGCGTGAATCGTTGATGTGAAGAAGCTTTGTTGTGAGATGTTCACATTGAGACTCGCATTAGATTTTGA GGAGATACTCTTGTTTACTAAGTTGTGTGGATGTATTTCATCTTCCAGGTAATTATGGGAGATTTCTCGATTCAGATCAG TTCTAAGCTGATCAATCAGCTAGCTGAAGGTAACGATCAACCAAAGAGGAGGGCAAAGAAAACCAAACCTAAAGTCTCAC CACAGAGCAAACAGAAAACAAACCAAGATGAAGAGAAGAAACCTAATCCAGTGGCTGAATTACCGATGCAGCCACCGTTT TTCTTCCCAATACCACCACAAGGAGCAGCAAGCACAGAGCTGGAATCGATAAAATCCGTTGTGAAAGAGAGTGAGAAGGT TCTTGAGAAGCTGGAGTTACAAGAGAAGAACATTGTTCGTGAAGTTACGGAGAGAGCCAAGGATCTGAGGGAGAAAGAGT TTAAGATCCCGGAACCTAAACCAATGCCTTGTTCGTCAGATCACGAAGCCTGGATGAAATGCTACAAGGAGAACATCGGT AGTCCGTTGAAATGTAGCGGTTTCGTTAAGAGTTTCCAGGATTGTGCACGCCGGTCCAGACAGCAAGTGAATCCTGAAGA AAAGTAGAGATAATAAGATTCAAAAGGACTTTTTTGAGACACGAAGGGAATAGTTTTTGCTTTCTGAGATCATATGAATG ACACAGAAGAATTGTGCTTGTTTCCT .........................N......................N............................... ..............................N.......................i......................... ................................................................................ ...........................N.....................................N.............. ................................................................................ ................................................................................ .......................N.............................N....N..................... ...........N.................................................................... .........................................................................N...N.. .......................... Pos Score Pred ------------------------ 26 0.164 - 49 0.196 - 111 0.483 - 135 0.791 Yes 268 0.416 - 306 0.397 - 504 0.461 - 534 0.348 - 539 0.184 - 572 0.315 - 714 0.040 - 718 0.048 -

Explain the output -- Processing locus 1024: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTACAAATGACACATAACATGCAGACGCGGAGACGCGGAGGGCCGGTGTTGTTCGTCACTTGTCACTCTCTTCCAACAC CTAATCCAGACAACAACCTAAGATCTTCACTCTCGCACACACACGACACATGCATTCTTACACGTGATCGCCATGCAATC TCCTTTCTCACCTATAAAACTAACTCTTCACTTCACTCTTTACTCAAACCAAAACTCATCATCACAAACGAGTAAGAATA CAAACACAAATAGCAAAAAAATGGCAAACAAGCTCTTCCTCGTCTGCGCAACTTTCGCCCTCTGCTTCCTCCTCACCAAC GCTTCCATCTACCGCACTGTTGTCGAGTTCGACGAAGATGACGCCAGCAACCCCATGGGCCCAAGACAGAAATGTCAGAA GGAGTTTCAGCAATCACAGCACCTAAGAGCTTGCCAGAAATTGATGCGCATGCAAATGAGGCAAGGCCGTGGTGGTGGTC CCTCCCTCGACGATGAGTTCGATTTGGAAGACGACATCGAGAACCCACAAGGCCCCCAGCAGGGACACCAGATCCTCCAG CAGTGCTGCAGCGAGCTTCGCCAGGAAGAGCCAGTTTGTGTTTGCCCCACCTTGAGACAAGCTGCCAGGGCCGTTAGCCT CCAGGGACAACACGGACCATTCCAATCCAGGAAAATTTACAAGACAGCTAAGTACTTGCCTAACATTTGCAAGATCCAGC AAGTTGGTGAATGCCCCTTCCAGACCACCATCCCTTTCTTCCCTCCTTACTAATAGATTCCAAACAAAAACCCTCGAGCG TATGAGAGTGTGGTTGTTGATACATGTTAACACCACACCTCATCGTGTCTTTTATGAAAATGTAAGTTTTGG .......i...........i............................................................ .................................................N......................i....... ................................................................................ ....................i........................................................... .....................................i................N................i........ ...........................................i.....i.....i........................ ............N................................................................... ................................................................................ ................................................................................ ..........N..................................................................... Pos Score Pred ------------------------ 8 0.586 Yes 20 0.547 Yes 130 0.471 - 153 0.565 Yes 261 0.834 Yes 358 0.639 Yes 375 0.421 - 392 0.767 Yes 444 0.551 Yes 450 0.632 Yes 456 0.835 Yes 493 0.417 - 731 0.315 - 802 0.064 - 824 0.090 - 854 0.033 - 860 0.039 -

Explain the output -- Processing locus 1025: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAGACTTGTCAACGTGTCTTCACCAATTAAATAATTACACGTGTGCAAAGAACGTCTTTGACTCTCTTTTGTCTTACCC TAAACGGCAAAAAAACATTGACTACTGCAAGATTTTATCTCTTTCTCTCTCTCTCTTCTCTTTTCTGGTGACGCCATGGA TCTTTGTCGAAGCTCGATTTTGCTTTTGATCATTGCGTTGTTATCTCCTAGGACACTATCAATGCGTTACGAACTAAAGT CTTCGAAAACGAAATGTATCGGAGAGGAGATTCACGAAAACGCCATGTCCATTGGTAAATACTTCATCGTAAACCCTAAC GAAGATAATCATCCTCTTCCTGATTCTCACAAGATCATCGTCAAGGTGATGCCACCACAAGGGAAGAATTTGCACGAAGC CGATAAGGTAGAGGCTGGTCAGTTCTCGTTTACTGCTTATGAAAATGGGAGTTACGTAGCGTGCATCACTGCCATTGATT ATAAGCCGGAGACCACGTTGACTATTGATTTTGATTGGAAGACTGGTGTTCACTCTAAGGAGTGGACCAATGTGGCTAAG AAGAGTCAAGTTGATATGATGGAGTATCAGGTTAAGACCTTGATGGATACTGTTATTTCCATACATGAGGAGATGTATTA TCTTCGTGAAAGGGAAGAGGAAATGCAAGAACTGAATAGATCCACAAACTCGAAGATGGCGTGGTTGAGTTTCGGGTCGC TTGTGGTTTGTTTATCGGTGGCTGGTCTACAATTCTGGCACTTGAAAACTTTCTTTGAGAAAAAGAAACTCATCTAAGTA GTAGACATGCACACACATACTCATTTGCTGATCATAAGACCATTAGAGAGCTATATATATAGCTTTGGGTATACAACTGA TTTCATATTTTATTTA ................................................................................ ...........................................................................N.... .............................................................i.................. .............N..............................i................................... ................................................i............................... ......................................N.....N................................... .....................................................................N.......... ...............i..i.......................N.....................N.......N....... ......................N................................i........................ ................................................................................ ......N......................................................................... ................ Pos Score Pred ------------------------ 156 0.166 - 222 0.542 Yes 254 0.285 - 285 0.648 Yes 369 0.685 Yes 439 0.076 - 445 0.241 - 550 0.135 - 576 0.778 Yes 579 0.743 Yes 603 0.317 - 625 0.248 - 633 0.300 - 663 0.293 - 696 0.518 Yes 807 0.134 -

Explain the output -- Processing locus 1026: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATAATTTATTCCATAAAGCATTTTACACACTGAGAAAGTAATGATTCATGTCTTCTCTTTCGGCGAAAAATATAAAAAA ATAAACAATAATAATATATGAAAAAGGCAATTCCATTTATTTAGCATGCTAGAGAGCACCAAAAGAGACTCATCATTCAC AATGGCATATGCAACTATTCTCATGATCTTCTCGGTGGTGGCACTAATGTCCGGCGAGAGAGCTCATGCAGCAGTGGACT GCTCATCGTTGATACTAAACATGGCTGATTGTTTGTCGTTTGTGACTAGTGGTAGTACAGTTGTCAAGCCGGAAGGAACA TGTTGTTCAGGGCTTAAGACTGTGGTTAGGACAGGACCAGAATGTCTATGTGAGGCTTTCAAGAACAGTGGTTCTCTTGG TTTAACTCTTGATCTTTCTAAAGCTGCTTCTCTTCCTTCTGTTTGTAAAGTTGCTGCTCCTCCTTCTGCTCGTTGTGGCC TTTCTGTCTCTGGAGATCCTCCTGCTACTGCCCCTGGTTTATCTCCTACGGCCGGAGCAGGAGCACCCGCGTTGTCCAGT GGTGCAAATGCGGCAACTCCAGTTTCGTCCCCGAGGAGTTCCGATGCGTCCTTGCTCTCTGTATCTTTTGCATTTGTCAT CTTCATGGCACTCATTTCTTCTTTCTATTGAGTCTTAAAATTTAATTGCTTCTTGTTGAGTTATTTTTGTTTGCTATGTA GCTGAAGAGTTTGGTGTTACTGATCATATTTACCTTTCATGTGTCAATTT .........................................i......N............................... .................N...........................N.................................. ....................i..........................................................N .........................................N.....N................................ ................................................................................ ................................................................................ .......N...................................N.................................... ....N......................................................................N.... ......................................N........... Pos Score Pred ------------------------ 42 0.533 Yes 49 0.433 - 98 0.471 - 126 0.298 - 162 0.621 Yes 169 0.131 - 183 0.211 - 207 0.497 - 226 0.165 - 261 0.608 Yes 320 0.206 - 362 0.210 - 368 0.090 - 568 0.074 - 604 0.137 - 645 0.084 - 716 0.047 - 759 0.039 -

Explain the output -- Processing locus 1027: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGAAACAAAAACAACGAAGAAGAAAGAAAGAAAGAAAACATAGTGGAATCAGAATCCGATCCCTTTCTAACATCACTCTT CCTTTTTTGAAAAACTTGTAAAAGTTTCCAACTTTCCTCTTTTCAACAAGTTGTAATTTCTTCGCTTGTTGCATGTTGAA ACACGTTATTCTGCAATTAACAAAGATAAAATCACATAGATTTTGCATTATAAAAAGCTCGTTAATGTCACTATCTTCTC TGCACAGATCACCTTTAGTACTTCCCAAGGTGACATCCATGGCAAAGAACTCATCATCACAGAGTAGTAGCAAGAAGAAG CAAGAAAACAGAGTGTGCGAGAAGATCTTCAGAGCAGTGACTAGTCCTGTCCGAACCGTTCGTCGCATCTCCACCAAATC TTCACCAGCAGAGGCTGTTCGCGTCAAATTTGCAGAGACATCGGCTAGGCCTAGGGCAGCCAAACCCATTACAAAGGTGG AACCGGTGATCGCTCGAGTGGATACTACGATCAAAACGGACGAGAGGTTCACCGAGTATATAAAGAAAGCGAAGCTGAAG ATAAGGGCGATGACGAATGTCGGTGATATGATGAAGGAGGATGCGTGGGAGACAAGTGATGAAACAGATCAGACACGTCA TGATCATCTTCACCACCATGTGCCTATCTCTCACGTAGGTAGTAGTGGCCGGAGATCATCAGATCAGTTCTCTGAGTACA TAAAGAAGGCAAAGATGAAGCTCAGTTCCTCTTCCACCATTGCTCGTGCTAACCCGACTTTCAAGTGATGGATTTCGACA AATTAATGATTTGAATAATCCTCTGGCCTGAGAGATTGATATATATGTATATGTTTTGTGGTGTGTGTTGACAAGTACGG ACATGGA ................................................................................ ........................................................................N....... ................................................................N............... ......................................i......................................... ................................................................................ ................................................................................ ................................................................................ .........i......i..........i..i.........i.................N....................N .................N.............................................................. ..............N....................................................i............ .....N......................................N.....N............................. ..N.... Pos Score Pred ------------------------ 153 0.259 - 225 0.313 - 279 0.511 Yes 570 0.676 Yes 577 0.578 Yes 588 0.593 Yes 591 0.516 Yes 601 0.514 Yes 619 0.451 - 640 0.183 - 658 0.125 - 735 0.362 - 788 0.522 Yes 806 0.082 - 845 0.042 - 851 0.038 - 883 0.060 -

Explain the output -- Processing locus 1028: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCTTCTAGTCAAAAAATTGAATATTGACCCTTTCATTGTTATGTTAACGTTTCATCTTATAACCTATAAATTTACATT CGTTTTGGGCCTTTTGGCTAATTACGAGATAGACCCTTGTCAATGTGGAAAAAAAAAAAAAAAACTTCGAACTGGGTAGT TTTGTCGAACAGTTGGTGTCCATCACTAGAACACCGATCACCGATGAGAGGCGTCACACCCATAAACCCTCAGAAGAAGA TAGAAGCTGATTCGAATCCCGTCAAAGAATCTGATTACTATGAAGGCGAACGTCTTACTCATCTACTCGACTTGATTCAG AGAGGAATTGAGACGTCCAAGCTTTCGAATGTGAACTCATTACCTGAGAAGATATGGCTCAAGAAGCAAATAGCGATTGG GATCAACGAAGTCACTCGAGTTCTTGAGAGGATGAATCCCAACAATACTAGTGACCAACAACAAAACCCTAAACAACTTC AGGTAGTGATTTTAGTAGCAGATTGTAAACCGAGGATGCTGACCAAGCATATACCCAACTTAGCTGCTTCGAGAAACGTT CCGGTTCTCTATGTAAGGGACAACAAACGAGCCTCTCTCAGATTAGGTGAGTTGGTTAAGCTCAAGACGGCTCTAGCCAT CGGTATCAAGGCTAGAGGAAATGATCTCAACCTACTACTTAAACAGATTCTTCCGAGAGATTCTTGATCCTGTAAGAGAA GGAAGTTTTCTTTGATCTGGTTTTGATGTGATCCTTTCTCAATGTACTAGTAGTAGTATATACAGATAAATGAGATTGAC AACAAA ..........................................N..................................... ..........................................i..................................... ...........................................i.................................... .......................................N........................................ ............................N........................i.......................... ...............................N................................................ ...................................N............................................ ..........N..................................................................... ....................N........................................................... .........................N...............N...........................N.......... ...... Pos Score Pred ------------------------ 43 0.309 - 123 0.582 Yes 204 0.518 Yes 280 0.188 - 349 0.172 - 374 0.648 Yes 432 0.479 - 516 0.205 - 571 0.137 - 661 0.229 - 746 0.042 - 762 0.081 - 790 0.066 -

Explain the output -- Processing locus 1029: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GATGCATATAAAGAAACATAGACATCATCGCCATCCATCCTCAGATCAGTTCCGTAGTTTACTGGATCACGACACACACA CATATACACGTGTACATATAAGCCATTCTTATCAAAATGACCACCTTCCTCCTGAATTCTCATTCATTATATAGGCGTAT ATTTCGGAATTCTAAGTTTTGTTGCTACGATCGAGCCAGCGTTCTGTTTCTGTACGTCTCTCTGGGTAAGAAAGAAAAAA TGAAGGACGAAACGGGAAACCCTCTGCATATCAAGTCTTTGAATCACATCTCTCTTCTCTGTCGATCCGTCGAGGAATCC ATTAGCTTTTACCAAAACGTTTTAGGGTTCCTCCCTATTCGCAGACCTGACTCTTTTGATTTCGATGGCGCGTGGTTGTT TGGACATGGAATTGGGATTCATCTTCTTCAATCTCCTGAACCTGAGAAATTACTCAAGAAAACAGAGATTAATCCCAAGG ATAATCACATTTCTTTCCAGTGCGAAAGCATGGAAGCAGTGGAGAAGAAGCTCAAGGAGATGGAGATAGAGTATGTGAGA GCAGTGGTTGAAGAAGGTGGCATCCAAGTGGACCAACTCTTCTTCCACGACCCTGATGCCTTCATGATCGAGATATGCAA CTGCGATAGCCTCCCCGTTATCCCTCTCGCTGGAGAAATGGCTCGTTCATGCTCTCGACTTAATATCCGCCAGCTCGTTC AGCCAACTCAGATCCACCCCTAGTTCTCTCTGTCTTCCCTAATTTCTGTTTTCGTAAATAATTTCAAGTGTCCATGTGAT TGTCTCTTTGATTGCGTGTGAATATAATTGCATGTAATAAAC ....................................N........................................... ...............................................................................i ................................................................................ ................................................................N............... .....N.......................................................................... .............................i.............................N............N....... .......................................................N.......N..........i..... .....................................i..........N............................... .........................................................................N...... ...............................N.......... Pos Score Pred ------------------------ 2 0.446 - 117 0.201 - 240 0.539 Yes 385 0.307 - 406 0.372 - 510 0.806 Yes 540 0.496 - 553 0.444 - 616 0.284 - 624 0.413 - 635 0.623 Yes 678 0.717 Yes 689 0.497 - 794 0.046 - 832 0.055 -

Explain the output -- Processing locus 1030: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATGCCAAATTGTATCAGTGAAGAATTTAAATGTCCATATTTATTCTTCTATAATAAAATTTTGGGTCTAATGGGCCG AGAACTACATGAAATAGTTAAAAAGCCCAACTTAGAATTTTAGAAGTTTTTTTTAGGGTGGAGGGAAATATTGGCGCCAC TCACTCACACACACTCCATTTAATGGCGGAACTACGACGCTTCTTCGTCATCACAACAACAACAATTCGACCTCAATTGA AATTAGGGTTTCTCTGCAACTCCACAAACAGACTCTTAACAAAACTTCGATTCCCAATTTCAACAATGGCTTGCGTAAAC GAACCATCACCTAAACTCCAAAAGCTCGACCGAAACGGAATCCATGGCGATTCATCACCATCACCATTCTTCAAAGTGAA GAAACTCTCCGAGAAAGCTGTTATACCAACACGCGGCTCACCACTCTCCGCTGGATACGATCTCTCAAGTGCGGTGGATT CAAAAGTTCCAGCGAGAGGTAAAGCGCTTATCCCAACGGATCTAAGTATCGCTGTTCCCGAAGGAACTTACGCGAGAATT GCGCCGAGATCTGGTTTGGCTTGGAAACATTCGATTGATGTTGGTGCTGGTGTGATTGATGCTGATTATAGAGGACCTGT TGGTGTGATTTTGTTTAATCATTCTGATGCTGATTTTGAGGTTAAGTTCGGTGATCGAATCGCGCAGTTGATTATCGAGA AGATTGTGACTCCTGATGTTGTTGAGGTTGATGATTTGGATGAGACTGTTCGTGGTGATGGTGGTTTTGGTTCTACTGGT GTCTAAGTGATAATCATCTTCTTCGTTTATGAATGTGTTTTGGTTTTTAATTTCGTCGAACAGTTTGTGGGCATTATTAT TGTACTATCTCTTTGCTTCATCTTT ...i............................N.......................................N....... ........N....................................................................... ......................N......................................................... .................................................................i.............. ...........................................i.................................... ................................................................................ ................................................................................ .....................................N....................N..................... ..........................N..................................................... ...............N..............N........N.................N...................... ............................N...N............................................... ......................... Pos Score Pred ------------------------ 4 0.545 Yes 33 0.217 - 73 0.336 - 89 0.335 - 183 0.367 - 306 0.645 Yes 364 0.726 Yes 598 0.190 - 619 0.059 - 667 0.057 - 736 0.104 - 751 0.048 - 760 0.115 - 778 0.070 - 829 0.049 - 833 0.090 -

Explain the output -- Processing locus 1031: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAGTAACAAGATGAATACCTCTTGTTGGCATCTTAAATCACACGAATTGTTCATGTTAAAGAAGAAGTTTCAAATTGTT TGTTGATATTGAAGAGAATGAGAACTATTTTCTATGTTTACTTTATTTTGCTATTTTCATTTTTTTCTTTCAGTTCCCTA CTCTTATCTGTCTTGTGATTCCAAATGCAGAACACACACAGAATCTCCCATGGCTGCATTTGCAACGGCAGAAGCGTGTG ACAGCAACGCAGAACTAATATCAAATGGAGACTTACGCGCTCTTCACCCAATCTTCAAGATCTATGGCCAAAGAAGATGC TTCTCAGGACCAATCGTGACTCTCAAGGTCTTTGAAGACAATGTCCTCGTCAGAAACCAACTAGAAACGAAAGGAGAAGG CGGAGTCTTAGTTATAGACGGTGGTGGAAGCATGAGATGCGCGCTTGTTGGAGGAAACCTCGGACAGTTAGCTCAGAACA ACGGGTGGTCGGGGATTGTTGTGAATGGATGCGTTAGAGATGTGGATGAGATCAATGACTGCGATGTTGGGGTCAGGGCA TTGGGATCTAACCCGTTGAAATCTACTAAGAAAGGTCATGGTGAGAAGAATGTGCCGGTTCATATTGGAGGAACTTTGAT TAGAGATGGAGAGTGGCTATATGCTGATAGTGATGGTATCTTGATCTCCAAGACCGAACTCTCTGTTTGAGTCAGAACGT TTCCGTGATAGTGCTAAGAGACTACAGCTTCTTGGAGCAAGGTGGTTTATTCGAATTCTTAAGAATCTGTTTAATACTCC AAATAATCA ...........N.........................................N.......................... .................N...............N.............................................. ........................i........................i.............................. ........................i......................................i............N... ........................................i....................................... ...............................i....N........................................... ........................N...N..........N.....N........N........N................ .....................................N...........N.............................. .....i..............N...........N............................................... ................................................................................ ......... Pos Score Pred ------------------------ 12 0.260 - 54 0.067 - 98 0.198 - 114 0.358 - 185 0.553 Yes 210 0.740 Yes 265 0.600 Yes 304 0.702 Yes 317 0.393 - 361 0.647 Yes 432 0.551 Yes 437 0.443 - 505 0.330 - 509 0.491 - 520 0.259 - 526 0.093 - 535 0.339 - 544 0.102 - 598 0.347 - 610 0.197 - 646 0.570 Yes 661 0.214 - 673 0.240 -

Explain the output -- Processing locus 1032: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATTGAACATACATCGATTTATGTTTTTTTTTTATAATATAATGTAGGCCCAATGGCCCAGAACATAGCCTACTATTAT TGGGCCTGTAGCCTGCTTTAGCATATTTACCAATTATCACGTGTATGTATGTATGTATCATCTGTCCAGAGAAAATTGCT AATACCGGCGATGAATCAGCTGCAGCAACTCCAAAACCCAGGGGAATCGCCGCCGGTGCACCCTTTTGTAGCGCCGTTGT CGTATCTATTAGGAACATGGAGAGGACAAGGAGAAGGTGAATATCCTACTATACCGTCTTTCCGCTACGGCGAAGAGATC CGTTTCTCACATTCCGGCAAGCCGGTGATAGCGTATACGCAAAAGACATGGAAATTGGAATCAGGAGCTCCGATGCACGC CGAGAGTGGTTACTTTCGTCCAAGACCAGATGGTTCTATTGAAGTCGTCATTGCTCAGAGCACAGGTCTCGTCGAAGTCC AGAAAGGAACATACAATGTAGATGAACAATCAATCAAACTCAAAAGTGATCTTGTGGGAAACGCTTCAAAGGTGAAGGAG ATAAGTAGAGAATTTGAATTGGTTGATGGAAAACTATCGTATGTGGTTCGTATGAGTACAACCACTAACCCTCTTCAACC ACATCTCAAAGCAATACTCGATAAGCTTTGAATCCAAGTTCCTGTGAAATCGAAACCTGACACGACCGATGTGATATTGA GATGCTTTGATTTTGATATGTAGAATCACTCTGTTTAGTAACAACATTTG .....................N....................N..........i.......................... ............................................N...N...N........................... ..........N..................................................................... ................N............................................................... ...............................................i........................i....... .............................N.................................................. ...............N.....N.......................................................... .........................N..............N..........N............................ ....................................................................N........... Pos Score Pred ------------------------ 22 0.382 - 43 0.335 - 54 0.546 Yes 125 0.310 - 129 0.333 - 133 0.251 - 171 0.387 - 257 0.433 - 368 0.546 Yes 393 0.850 Yes 430 0.210 - 496 0.303 - 502 0.131 - 586 0.159 - 601 0.149 - 612 0.341 - 709 0.092 - 722 0.067 - 738 0.043 -

Explain the output -- Processing locus 1033: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGGAGACAAAAAAAAGAAGCCACGTAATATGGATTTAATTACCAAAACTGCCACTCGGCCGCCCCTTTGTTGACTTATA TTTGTCGTCTCCTCCTTATAAATGATGTCCTCACAAACTTCTTCACATAACAAACTCATCAAACTTCCTCAGCGTTTCTT TTTCTTACATAAACAATTTTTCTTACATAAACAAATCTTGTTGTTTGTTGTTGTCATGGCACCGACAGTTAAAACGGCGG CCGTCAAAACCAACGAAGGTAACGGAGTCCGTTACAGAGGAGTGAGGAAGAGACCATGGGGACGTTACGCAGCCGAGATC AGAGATCCTTTCAAGAAGTCACGTGTCTGGCTCGGTACTTTCGACACTCCTGAAGAAGCCGCTCGTGCCTACGACAAACG TGCTATTGAGTTTCGTGGAGCTAAAGCCAAAACCAACTTCCCTTGTTACAACATCAACGCCCACTGCTTGAGTTTGACAC AGAGCCTGAGCCAGAGCAGCACCGTGGAATCATCGTTTCCTAATCTCAACCTCGGATCTGACTCTGTTAGTTCGAGATTC CCTTTTCCTAAGATTCAGGTTAAGGCTGGGATGATGGTGTTCGATGAAAGGAGTGAATCGGATTCTTCGTCGGTGGTGAT GGATGTCGTTAGATATGAAGGACGACGTGTGGTTTTGGACTTGGATCTTAATTTCCCTCCTCCACCTGAGAACTGATTAA GATTTAATTATGATTATTAGATATAATTAAATGTTTCTGAATTGAGTATTATTTGATCATCATCTAAGTAGAAGCTTTAA GTTTAAGTGGTGCCA .............................i.................................................. .....................N..N....................................................... .......................................................i........................ .......................................................N........................ ................................................................................ ................................................................................ ................................................................................ ..............................N..i.........N..................................N. ..N...........N................................................................. .........N....................N................................................. ............... Pos Score Pred ------------------------ 30 0.710 Yes 102 0.299 - 105 0.398 - 216 0.862 Yes 296 0.325 - 591 0.368 - 594 0.702 Yes 604 0.252 - 639 0.357 - 643 0.137 - 655 0.238 - 730 0.087 - 751 0.098 -

Explain the output -- Processing locus 1034: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGATCGGGTCATTTTGCCACTAATCTACCACACATTTGATAACTAAAGAAGTAAAAAAGAATTTACACCCTAATTCCC TTTATTTCTTTAGCTTTATAACATATGATAATTTTGTTTAATAATAATTTATCAATATGTGTACCAAACCTCATTTTCTA TAAATATAAGCCAGCACATCTCTCTTCTTCTTACAAGCAAAAAAGAAAGAAATTAAAGAGAGAGAGAAAGATAATGGGAA AGTTAATGGCAAAGAGGTTTTGCATTGGGTTTGGGTGTGGAAGAATTGGGACGGGCAACAATAAAAGATCATCATCTTCT TCTTCTTCTTCATCGTCATTTTCATCATTATCATCATCATCTTCTTCTTCATCGTCATCATGTAACAATAACAACATAAA ATGGGAGATGAGGCCTGGAGGGATGCTGGTTCAGAAGAGATCAGAAGATTCTAACACTGAAGATTTAATCAGCCTTAGAG TCTCCACTGTCTCTCAGCTTTCATATGAAATCTCCATTGATGCCAACTCTACTTTCGGTGAATTGAAGATGATGATAGCA ATAGTGAGTGGAATTGAGGCAAAAGAGCAAAGACTCTTGTTCAGAGGAAAGGAAAGAGAAGACAGAGAATACTTACACAT GATTGGTGTTGGAGATGGAGATAAGGTCTTTTTATTACAAGATCCTGCTTTTAAGGAGTTGAAACATATTCATCTCCCAA TTACTGTCTAACTAATTCCTTACTTTATTAATTGATTAATCAATCGATTAATTAGCTCTAATCCCCACTTATTAAACACT CTCTTTTATAAGTGCTATAAGTTCTTGATT ................................................................................ ........................N...............................i....................... .........................................................................N...... .....N.......................................................................... ...........................................................N.................... i......i..............i......................................................... ........................N..............N............................N..N........ ..............................................................................i. ..............N................................................................. ................................................................................ .............................. Pos Score Pred ------------------------ 105 0.319 - 137 0.761 Yes 234 0.425 - 246 0.290 - 380 0.179 - 401 0.722 Yes 408 0.694 Yes 423 0.750 Yes 505 0.339 - 520 0.465 - 549 0.471 - 552 0.229 - 639 0.688 Yes 655 0.083 -

Explain the output -- Processing locus 1035: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTTCTAAGTAGTTCAATAATTTTATAGGTGAAAAAATAGATCGTGTTTTTAAATAATAAATAAAGTTATATAAATACACG TTTTAGCTGCATGATTGTTAGATGCTTTTATATTGGTTTGACCCAAATGAAGAAACCATGGTCCACAATTGTCCCCCTAC TAGTCTATATGAACACCTCTTCTCACCACACACACCAAACATTCATCACAATTCCCTAAATCCTACAATCTTTCTTTTCT TCCATCACCTAAGAGTAAACCCTACCTTGCGTCCCAAGTCTCCACCGATAATGCAACCATCCACCAACATTTTTAGCCTC CATGGTTGTCCACCTTCGTACCTTTCCCATATTCCTACATCCTCACCATTTTGCGGACAAAACCCTAATCCATTCTTCAG CTTCGAAACTGGCGTGAACACTTCACAGTTCATGAGTTTGATCAGCAGCAACAACTCGACCTCAGATGAAGCAGAAGAGA ATCATAAGGAGATAATAAATGAGAGGAAGCAAAAGAGGAAGATATCTAACAGAGAATCGGCAAGAAGATCGCGTATGAGG AAGCAGAGACAAGTGGATGAGCTTTGGTCACAGGTGATGTGGCTTAGGGATGAGAATCATCAGCTGCTTCGTAAGCTTAA CTGCGTTTTAGAGTCTCAAGAGAAGGTTATTGAAGAGAATGTTCAGCTCAAAGAGGAAACCACTGAGCTTAAGCAAATGA TTAGTGATATGCAGCTTCAGAATCAGAGTCCTTTCTCTTGCATCAGAGACGACGACGACGTTGTATAGATGATTTTTATT TTTATTTTTTTGGGGCTCTAGTATGTATCAACAAATGTATATCCAGAGATCTGTCTCAGCTTAAGATTTAATTAAGACTC TAAATTA ................................................................................ ..........N..........N........................i..........N...................... ........N....................................................................... ..................................................i............................. ...............................i.................................N.............. ..................N.......................................................N..... ................N...................i............N.............................. ......................................N.....................................N... ........N...........................................................N........... ......................N...........N............................................. ....... Pos Score Pred ------------------------ 91 0.379 - 102 0.412 - 127 0.517 Yes 138 0.496 - 169 0.192 - 291 0.658 Yes 322 0.719 Yes 432 0.568 Yes 466 0.276 - 499 0.276 - 555 0.442 - 577 0.287 - 597 0.518 Yes 610 0.230 - 679 0.216 - 717 0.433 - 729 0.251 - 789 0.252 - 823 0.110 - 835 0.182 -

Explain the output -- Processing locus 1036: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTCTTCTCACTAGTTTCTTGTATAATAAGAAGTTTTTGTGAATGTATTATTAGCAGTTAATGTGTTCATTGATTCTTT TGGAAGTGATTTGGGGATTGATGGATTTTGCTTCTTATTATGGTTTATACACAGATGAATAGGGAGAAGTTGATGAAGAT GGCTAATACCGTCCGCACTGGCGGAAAGGGTACTGTCAGAAGAAAGAAGAAGGCTGTGCACAAGACCAATACAACTGATG ACAAGAGGCTTCAAAGCACTCTTAAGAGAATTGGAGTTAACTCCATTCCCGCTATTGAAGAAGTTAACATCTTTAAGGAT GATGTTGTTATTCAGTTCATCAACCCTAAGGTTCAAGCTTCAATTGCTGCAAACACATGGGTTGTTAGCGGTTCTCCTCA GACCAAAAAATTGCAAGATATCCTTCCTCAGATCATCAGCCAACTTGGACCAGACAACATGGACAACCTGAAGAAGCTAG CAGAACAGTTCCAGAAACAGGCTTCTGGTGAAGGTAATGCCGCCTCAGCAACCATACAAGAAGAGGATGATGACGATGTC CCAGAGCTTGTTGGAGAGACATTCGAAACTGCTGCTGAAGAGAAAGCACCAGCTGCTGCTGCTTCTTCTTAGAGAGAAAA GAGCGAGACCACATCCAAAAAAAAACCGCACTTTGATTTTTACATATCTTTATAATATGTTTGTTCGCTCGATCCTTTTT GTAGTTTCCGA ...........................................N.................N.................. ....................i..................i..............i.................N.....i. .............................................................................N.. ..............................................................................N. ..........................................................i..................... ....................................N.............................N..N.....N.... ................................................................................ ........................................................N....................... ........... Pos Score Pred ------------------------ 44 0.384 - 62 0.475 - 101 0.563 Yes 120 0.739 Yes 135 0.597 Yes 153 0.444 - 159 0.870 Yes 238 0.095 - 319 0.294 - 322 0.118 - 377 0.774 Yes 459 0.883 Yes 517 0.319 - 547 0.128 - 550 0.447 - 556 0.353 - 697 0.066 -

Explain the output -- Processing locus 1037: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGCTCTGCATTTGTATTAAGATTGTGATGTTGATAATTAAAACGACTAAATAATTTTTGCCAGGTTGGATTATAATGAAC AAGTCCAAAATGTTATTTTAATTTTATTTTCATGGCAAGCACGTTCAAGAAATGGCTCCAACCAAAAACCAAACAAACAT AACAGTATTTAAAACACAACTAAAACCTCAAAGAACAGAGAGAAGAAAAACAAGAATTAAGAAAATGGGGAAACCAGCAG GGTATTGGGTAAATAAGATTAGGACATCATTCAAAGGAGGATCATCGTCGAGCAAATCATTAGATGAAGGAAGTACTTCG GGTTCGAGAAAGAACGGTTCGAAGCATAGTAAGAATCTGAAAGCAGAGGATGCTAATAAGAATGGTGAAGGAGGAGGAGT TCAAGCTGAGAGTGGGAGGAAAGTGATGGTTGTTGTGGACACAACTTCACAAACTAAGAATGCTCTTCAATGGGCTTTAA CACATTGTGTTCAAGATGAAGATAATATCACTCTTCTCCATGTCACAAGAACACCCGTAGGACAAGCTATAGATGAGACA CAAAGGGAGAGAAACTCAAGGGCTCATGAACTAGTTCATCCATTGAAGAATTTTTGTCAACTCAAGAAACCTAACGTCAA AACGGAGATAGTGGTGGTTGAAACGGCGGAGGAGAAAGGTAAGACGATAGTGGAGGAATCAAAGAAACAAGGAGCAGGCG TTTTGGTGTTAGGGCAGAGAAAGAGAACCTCGAAGTGGCGCGTGATTTGGAAATGGCGTACGAAGGGAGGAATGGGAGGA GGAGTGGTCGAGTATTGTATTCATAACTCCGATTGTATGGCTATTGCCGTAAGGAAGAAGAGTAACAATGGAGGTTACTT GATCACCACGAAACGTCACAAAGATTTCTGGCTCTTGGCTTAATTTTTATTTCAAACAAATTCATCCCAAAATCTTTTTT TTTTTTTTTTTGTAATCGAAACGAATCTTTTTAAAGAATTCATGTTTTATGGATTAATTGAT ..........................N...............................................N..... .........N.....................N...................i............................ ................................................................N............... ...............................................................N................ .................................................N...........N.................. .........................i.................................N.........N.......... ...............N.......................N................................N....... .........................N...................................................... ................................................................................ ....................................................N..................i........ ....................................i..............................N............ ................................................................................ .........................................N......N............. Pos Score Pred ------------------------ 27 0.180 - 75 0.324 - 90 0.478 - 112 0.463 - 132 0.760 Yes 225 0.488 - 304 0.328 - 370 0.118 - 382 0.222 - 426 0.627 Yes 460 0.414 - 470 0.455 - 496 0.383 - 520 0.156 - 553 0.193 - 586 0.062 - 773 0.496 - 792 0.684 Yes 837 0.506 Yes 868 0.251 - 1002 0.058 - 1009 0.053 -

Explain the output -- Processing locus 1038: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGTTGTGACTATTGCTCAGTATTACTGTTTTTGTCATTGGCTAATTAATAACAGTATTAGTTATTGTATATGCTTAATTT ATAAGATACATTTTGGGCTAAATGGGTTTAGCTTTGTGTGTTTGTTTTGAAAAACAGATGAATAGGGAAAAGTTGATGAA GATGGCTAACACTGTCCGCACTGGCGGAAAGGGGACAGTAAGAAGAAAGAAGAAGGCTGTTCACAAGACCACTACAACCG ATGACAAGAGGCTCCAGAGCACTCTTAAGAGAGTTGGAGTCAATTCCATTCCCGCCATTGAAGAAGTTAACATTTTTAAG GATGATGTAGTCATTCAGTTCATTAACCCTAAAGTTCAAGCTTCAATTGCTGCTAACACATGGGTTGTGAGTGGTACACC ACAGACGAAAAAATTGCAAGACATTCTTCCTCAGATTATCAGCCAACTTGGACCAGATAACTTGGACAACCTGAAGAAGC TAGCAGAGCAATTCCAGAAACAAGCTCCAGGTGCAGGTGATGTCCCAGCAACAATCCAAGAAGAGGACGATGATGATGAT GTCCCAGATCTTGTAGTGGGAGAGACTTTCGAGACCCCTGCTACTGAAGAGGCTCCCAAAGCTGCTGCTTCTTAGAGGAG GAGGAAGAAGAAGGAGAAGAGCTCACCTGCAAAACCCATCATAAAAATGTTTGTCGCTCGACCTCTTCTGAGCACTGTCA GATTCTTGTTTTCT .....................................................................N.......... .....................i...................................i.................i.... N............................................................................... ................................................................................ .......................................N.............................N..N..N..N. ................................................................................ ..............................................N................................. .............. Pos Score Pred ------------------------ 70 0.252 - 102 0.543 Yes 138 0.603 Yes 156 0.686 Yes 162 0.791 Yes 241 0.133 - 322 0.261 - 325 0.132 - 380 0.773 Yes 520 0.411 - 550 0.160 - 553 0.237 - 556 0.204 - 559 0.238 - 687 0.075 -

Explain the output -- Processing locus 1039: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAACTTTCAATATAAATTCAGTAAATGTGAGTATATATATTACATACAGTATACTTTAAAATTCTTCAATGACGTATTT ATATTTCTATAGTTTTTTTAACAAATGGTGAGAAATAAAAAGAAGTGGTGTACACAATCTAACACGTTCCACTGGTTCAC TCTACAAAGTGACCAAACACTTGCCCTTCACACTTCTTTGTCATCTTCCCTCTTTCCTTCTTCTTTCTTCCTCAAATTCA CTTATTCACATTCTCTCACCAGACACAAACTCCAGATTCATCCGACAATGGAAACTTTCAGAACATTCCATCTTTTTCTC TTCTTCTTCTTCTTCACATTCACTACTACCCTGACGTCACCATCTCAGATCGCAGACTGTACAATGTGCACTTCTTGTGA CAACCCTTGTCAACCAAACCCTTCTCCTCCTCCTCCACCGTCAAATCCATCTCCTCCTCCTCCATCTCCCACCACCACCG CCTGTCCTCCACCTCCTAGCTCCAGTGGTGGTGGTCCTTACTACTACTACCCTCCTGCTTCTCAATCTGGCTCTTACCGT CCACCACCTTCTTCCTCCAGTGGTGGCTACTACTATCCACCTCCTAAAAGTGGAGGAAACTACCCTTATACACCGCCTCC AAACCCTATCGTTCCATATTTTCCGTTTTACTATTACAACCCTCCGCCGCAATCTGTTATGTCCGGATCCGATGCAAAGA TCAGATTCTCTTACGGAGTTTCATTTATTTTAATTTTCTCTCTTTATTTCGGTTGCTTCTAGTTTAAAACATAATTAAAG AAGATAATTAAAGGAAGAAGTAGCTTTATTAATATATCAACCAAACAGGTTCATTTCCTCAGATCCTTGCAGAGATTTTT .........................i...........................................N.......... ........................N....................................................... ................................................................................ ...............................................N................................ ...............................................................i................ ................................................................................ ................................................................................ ................................................................................ ..........................................................N............N........ ................................................................................ ................................................................................ Pos Score Pred ------------------------ 26 0.507 Yes 70 0.266 - 105 0.354 - 288 0.493 - 384 0.704 Yes 699 0.317 - 712 0.241 -

Explain the output -- Processing locus 1040: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAACTATGTGGGATGATTCCAGGCCCAATATTCCAAGCCCAATCGTTTGGTCATATAAATAAGAAACTGATCTCTGTTG TGCGTCAAAACTATGTATTAGAATTTCATTTTGTCCAGGCCCAGAATGAATTATAGGCCCAATAGTCTATTCTCTCTCCC ACGAACATTATTTATAGATTTTTGGGTTTCCCCTAAATCTCGAGCTTTTTCTTCCCTTGATTTCTTTGGTCTCTGTCCGT ACGACGTCGAATATCTAGATTGTTCGACGGCGATGGGAGCTGGGAGAGAAGTCAGCGTATCGCTAGATGGAGTCAGAGAC AAAAACTTGATGCAGCTTAAGATACTCAACACGGTTTTGTTCCCGGTTCGTTACAACGACAAGTACTACGCCGATGCAAT CGCTGCCGGCGAATTCACCAAGCTTGCATATTACAATGACATATGTGTTGGAGCTATCGCCTGTCGGCTGGAGAAGAAAG AAAGTGGGGCCATGCGAGTTTATATAATGACGCTTGGTGTTCTTGCACCCTACCGTGGGATTGGCATTGGCTCAAATCTA TTGAATCATGTTCTTGACATGTGCTCCAAGCAAAACATGTGTGAGATATACTTGCATGTGCAGACAAACAACGAAGACGC AATCAAGTTCTACAAGAAGTTCGGCTTTGAGATCACAGATACCATACAAAACTATTACATCAACATTGAGCCAAGAGATT GCTACGTTGTCAGCAAGTCCTTTGCTCAATCTGAAGCCAACAAATGATGAAAAATACCAAACTTGGGGAAGCCATTCCTC CCCAGTTTCTTTGTTGCATTCAGTTCAAGATCTATTAATGTTATGATGATCCTTCGGTTTCCGAGT ......i......N.................................................................. ............N................................N.................................. ................................................................................ ................................N.................................N............. .........i...............................................................N...... ...................................N......i..................................... ...........i..............N..................................................... .......N..........i.................i..................N........................ ................................................................................ ...........................................i..N................................. .....................................N....N..N.................... Pos Score Pred ------------------------ 7 0.509 Yes 14 0.221 - 93 0.366 - 126 0.202 - 273 0.366 - 307 0.462 - 330 0.699 Yes 394 0.350 - 436 0.361 - 443 0.602 Yes 492 0.624 Yes 507 0.484 - 568 0.207 - 579 0.619 Yes 597 0.635 Yes 616 0.230 - 764 0.773 Yes 767 0.446 - 838 0.039 - 843 0.026 - 846 0.042 -

Explain the output -- Processing locus 1041: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATGTAACCCATCTCATAACAGATTTATAATTTCGTGTATAATATAATCTCCAAATCACTAATTATGACCTCACATTTT AGGATAATGTTTTTTTTTTAAATGCTTATGGTCAAGAAAGAAGATAACGATTCAAGCCACACGAAACAGAAGAAGACAAT GGCCTCTCTCTTCACACCATCACCAACCACCACTAGTAGTAGTGGAGACACCATCTATGTAGCGGCGGTGCCGCTTAAAG CCGCGGCCGGTCCGCCGCAACTTATTATGTCAATGGCTTACTCACTTAATATCTCAAATTTACAACATTTCATGGTCCTC ATCAAACCTTCTTCTCCAATCCGTCAAGAGGTTCTTGTGTTTGATTTTCAGCCACGTAACCCTGAAAGCATAGAAGCAGC AATCTCACTTTTGTCAGGAAATTTGATTCCAGGTGTAGTTTTGCAAAGAAGGTTGAAGAATGTTCCAAGACAAAGATGTT GGATGGTTGGACCATCTAAAGGGAATGATGCTATGGAAATGGCAATGGAGTTTAATAAGTCATGGGAGACTGATTTGAGA GTTGGATTTCATGATTGCCGACATTATACCAATGAACTTGTCCAACATCTTACCGGAGAAATGCAAATTGTGGAGCGACT TCCAAGAAGCTAATGCTATAGATGAAACAAATGGTCATTTATCTTATCTACTAAGATTTTAGACCCGACAATTATGATGT GATAATTAGTAGTTGAAGATAAATTGAATATT ..N..............................................................i.............. ......N..............N.....i..................................................i. ........................................................N....................... ..........................N.....N......................................i........ ................................................................................ ...........................................................N...............N.... ..N.....................N..N....N.....i.....i................N.................. ..........N....................N............................N................... ............N........N........N..........................................N..N... ................................ Pos Score Pred ------------------------ 3 0.122 - 66 0.783 Yes 87 0.233 - 102 0.423 - 108 0.577 Yes 159 0.872 Yes 217 0.198 - 267 0.157 - 273 0.450 - 312 0.626 Yes 460 0.326 - 476 0.291 - 483 0.467 - 505 0.196 - 508 0.296 - 513 0.476 - 519 0.584 Yes 525 0.636 Yes 542 0.425 - 571 0.251 - 592 0.289 - 621 0.245 - 653 0.395 - 662 0.124 - 671 0.082 - 714 0.032 - 717 0.053 -

Explain the output -- Processing locus 1042: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGATCTTCAAGGGAAATAAAAGTTTGGTGTGCAGCTGATGATTGATTGGTTCTGTGTAATTGTTAAATTGCATGATTAC GTAACTGAGAATTCGGTTTAACCGTTTAAATTCCGGTCTACTGAAATTGCTACGGAAATAAACCGAATTAAACCGAACCG GATCATGATTCAGTCTACTTTAGTTTTTGTGCTCCGTAGATTGAGAGAAGCCAATTTTAGGGAAAAGAGAGAGATGAGGA AGTTGAATCCGCTTCTGAAACGAGGCTTGAAGGCTATTGAAAATGGTGATTTGTTGAAGATCCTCCAAAGTGAAATTCGT CACGAAATCTCCCATCCTCGTTTCCAGGGCGTTGAGACCGGTTCTCTAGGAGATTTCAAATTGGATTGGGATTCACCGGA AAGCCAAGATATTGTTCTGAAGAGACAGTTTGATTCAGGCGAGAAGGTTGTGGTCTCTGCTTTATTGCAACCAGAACCTA TAGAACTAGAAGATGATTTAGTGTTTCCTAGGGAAGCTCATGCCAAAGTTTGTATCAAAAAGCCTGGTCTCAGCTCTATC TTGCAGTTTCATTGCCGGGTTTACGAATCAGGATCTGGATCTTCTCATTTCGACATCGAGAGCGCCTACTTTATCCGCTC TTTTGTTTCTGCTCCTTCTTCCACTTATGGAGACCATTTTTTCAGATCCCAAACTACACAGTGCACTGGAGCAATACCTA ATATCTAAGGGGGTCAGTGAAGGCTTAACTAATTTCCTTTTGTGTCACCTGAACAAGAAGGAGCAGGATCAATACGTTAA CTGGTTACGCAGGCTTGAATCAACTAT ......................................i.................................N....... ................................................................................ ....N....................................................................N...... ..........................................i..................................... ................................................................................ ................................................................................ ............N..........................N........................................ ................................................................................ ..........................N..................................................... ................................................................................ ........................... Pos Score Pred ------------------------ 39 0.554 Yes 73 0.168 - 165 0.311 - 234 0.411 - 283 0.505 Yes 493 0.401 - 520 0.299 - 667 0.344 -

Explain the output -- Processing locus 1043: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CACAAGATCAGATCCTTCTGTCTCATCATCATCATCATTATTATAATCATAATTACAAGAAGAATCGTTGTTCAAAATAA TTAACGTATAACGACTTCTAGATGAATAATTTTCAGGAGAAGAAAAGAAGGCGTAGTAAAACGCCGAGAGTTTGTAATAA CGAAGAAAACATGGAGAAGTTAGTACATAAAGAAATTGAGAAACGAAGGAGACAAGAAATGGCTTCTCTCTACGCGTCTC TCCGCTCTCTTCTCCCTTTAGAGTTCATCCAGGGAAAACGTTCAACGTCAGATCAGGTGAAAGGGGCAGTGAATTACATA GACTATTTGCAAAGGAATATCAAAGACATAAATTCCAAGAGAGATGACCTCGTGTTATTGTCTGGACGAAGCTTTAGGAG TAGTAATGAACAAGAATGGAATGAGATTTCGAATCATGTCGTGATTCGTCCGTGTTTGGTCGGCATAGAGATAGTGTTGA GCATTCTCCAAACGCCATTCTCAAGCGTTCTGCAAGTGCTTCGTGAACACGGTCTCTATGTTCTTGGCTACATCTGTTCC AGTGTAAATGATAGACTCATTCATACTCTACAGGCTGAGGTCAACGATCTGGCTTTGATCGACTTAGCAGACCTTAAGGA TACACTTACCCTGATGAAGTAATTCAAATAATCAAACGTTTTTTTTCAGTAGATTCCTTTTAACTTCTCAAACCAAAACG ATGTATATATATGGTTCTTAGTACTTTTACTGTGTATTTAT ................................................................................ .....................i.......................................................... ..........i...............................................i..................... ................................................................................ ...........................................N.................................... .....N.........N....N..............N............................................ .........................................................N...................... .......N........................................................................ .............N.................................................................. N.........N.............................. Pos Score Pred ------------------------ 102 0.508 Yes 171 0.600 Yes 219 0.668 Yes 364 0.296 - 406 0.420 - 416 0.482 - 421 0.145 - 436 0.214 - 538 0.205 - 568 0.178 - 654 0.058 - 721 0.065 - 731 0.124 -

Explain the output -- Processing locus 1044: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTGTAGAAAATTAGAAAGCAACTCTTTAATTAGTTTCTTCACTAGCCTAAATATATTTCCCCTTCTCACTGTTTCTAC TTTTGATTGATTTACTTATAAATGCCGATTGAGGCAACATTCCTCCATACTTCAATTTCTTTCTTTCCGCTTAAGTTAAC GATTTGGAAAAACATGAGCGAGAACTCTTCGATTACAGTGGATCAGCCAACAACAGAAAATGGTAATGGCCAAGGAGAGC AGGTGATTCAATTGGGTAATATGCCGCCTCCTCTGTCACCACCCACCGCACGCAGAATCCTAGTGATCCCGCATGGAGCC GAAATTGTCAGTGTACTGGCATATATTTCTAGGGCTTCTCGTCAGGCAGTCTACATCTTGTCAGGGAGGGGATGCGTTTC ACGAGCCAACTTGGTGTCGTTACCAATCCGAGGAAACCGCATGGGTATTGGGAGAATACTTTACTTGAGTGGATGGTTCC AACCGATGCTCACTGATGTAGGTGTTGCTGTACATTCTAGTCGTCCCAACGGAATACTTCACGTCTCCCTTTCAGGTGTT TCACCAAGTGTTGCTATTGCATATCAACTTTTTGCTTTCGGAGATGTTGAGGTGTTGTTGACTCTTTTTACCATTATATA TCTCAATAGATTCTTTTAGATTTATTGATACCAAAAACTCTCAATACTTCTTGAAACACATTGTAGGTGGATGTTTTTTT TGTTTGCGGACCGGGGTCAATATTTCAACCGGATGCAC ................................................................................ .....................i.......................................................... .............i.............................................i.....i.............. ....................i...................................................N....... .......................................................................i........ ........................................i...............................N....... .....i.........N................................................................ ...........................................N.................................... ......................................................................N......... ................................N..... Pos Score Pred ------------------------ 102 0.638 Yes 174 0.598 Yes 220 0.732 Yes 226 0.611 Yes 261 0.627 Yes 313 0.420 - 392 0.553 Yes 441 0.611 Yes 473 0.147 - 486 0.567 Yes 496 0.226 - 604 0.219 - 711 0.050 - 753 0.028 -

Explain the output -- Processing locus 1045: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAACAAAGATGGCATTTCATCTTTTGACTGTGTGATATGTCAAACCCGCAGAATCCATAAAACTAGTTTACTTTGATA TGTTTTGTTCTTCTTCATTGAATGTTTGTGTGGTGTCTTGTAGTTGTAGGAAAGATGAACAAGCTGTTGGAATATGGGAG AAAAGCTATGTTCTATGTGAGAGTTCTCTCCGGCTATGAAGAAAGGAGAATCCGGAGTTACAGGTTGCAACTCGAAAAGC GTATCCAGCAGGCTAACCAAAGGAAAGTAGAGATAAACAGACTTCCAGAGCAGATTGTATTATCTGAAGTCCGCCGTATG GTTGAGGAGATGCAGAATTTGAATAAGCAGATTGAGAATACGGAGGCTCAAATTGAAGACTACTTCAAACCGATAGACAA GCATGCTAGTACCATAATGGAAATTCAGCTCGAAGGAGAGAAGAAAACGATGGGGACAATGATGAACGCAACACAACAGG AAGCTATACGAAAGATTGAAGAAGCTGAGAAACTCGCTCGGGCACGTGGAAATGCAAATGTGACTACAGATGCAAACATG GGAGATAAGATCCAGGATTCAGAGTCCTCAGCCAACGAAAAATCTCAAGCAAAATGAAGTCAGATCTTGTCTTCATGTTG ACACAGAATAGATTCAGCGTCTCTGAATACATCCTCTTAGTTGATTAAACTGAGATTGTAGCCTCGCATTTGCTGA ..........i...........................N........................................N .....................N................................N..................i...... .......N......N....................N............................................ .............................................................................N.. .........i...................................................................... ..N.............i................................i........i..i.................. ...................................................N.....N...........N.......i.. .....................................................N....................N..... ............................................................................ Pos Score Pred ------------------------ 11 0.544 Yes 39 0.344 - 80 0.499 - 102 0.384 - 135 0.302 - 154 0.527 Yes 168 0.327 - 175 0.317 - 196 0.144 - 318 0.347 - 330 0.532 Yes 403 0.194 - 417 0.734 Yes 450 0.827 Yes 459 0.760 Yes 462 0.545 Yes 532 0.252 - 538 0.231 - 550 0.299 - 558 0.733 Yes 614 0.432 - 635 0.096 -

Explain the output -- Processing locus 1046: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTATGACTCTAATTGCTAGATCTGCGCTGGTTGTATGAATTTTTGGGATAATTTCGATTATTTTTCTTTGAACTTATA TATCTTGAATTCGTTTATTGAGATTTAAGACTTGTATTGATTTTCAAAGTTGTTTATATTGGGAGTCGAACATTAATCTA TTTTCTCTTCCTCTGGATAATGAAGCACTTGCCAGTTGTCAGTTTCAAAACCATGGTTGCAAATAAGAAGCTAACTACAA CCGATGCACTTGATTTCCTCCATCTTGTAAAAACTAAATATCAAGATAATCGTGAAATATATGATAGTTTTCTTACGATC ATGAAGGATTTCAGAGGTCAGAGAGCTAAAACATGTGACGTCATATCAAAAGTGAAAGAACTCTTCAAAGGGCAACCAGA GCTTCTCTTAGGTTTCAACACATTCTTGCCAACGGGGTTTGAAATAACACTCAGTGATGACGAACTTACGTCAAATTCGA AGTTTGCTCACTTTGACGAAGCATATGAATTTGTCAACAAGGTCAAGACAAGATTTCAGAACAATGATGTATTCAACTCA TTTTTAGAGGTTTTGAAAACGCATAAAAAGGAAAACAAGAGCGTTGCTGAGCTCTACCAGGAGGTGGCTATCCTTTTTCA AGGCCATCGAGACTTGCTTGAGGAATTTCATCTCTTTCTTCCACATTATGGATGAAGAATGATGAATTATGTATACAATG AAGCCATGCAAGTTGTGGTTCAAAGCCACTTTATCTGCTTCCATTTGACTCAATGGGGATTATCATTAGTTTGT ....N...............................N........................................... ................................................................................ ...................N................................i........................... ...N........................................................N................... N...............................i............................................... ........................................................N....................... ........................N......................................i..N............. ................................................................................ ...............................................N...N......N..N......N........N.. .....N..............................................N..................... Pos Score Pred ------------------------ 5 0.153 - 37 0.320 - 180 0.382 - 213 0.622 Yes 244 0.359 - 301 0.332 - 321 0.413 - 353 0.744 Yes 457 0.324 - 505 0.211 - 544 0.656 Yes 547 0.200 - 688 0.290 - 692 0.135 - 699 0.453 - 702 0.358 - 709 0.123 - 718 0.071 - 726 0.130 - 773 0.052 -

Explain the output -- Processing locus 1047: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTCTCATTGATTACCGGTTTGTGTTTGATATAGAGTTACGCGTATAAGTACACTTTCATTCTTTTATTTCCTTCTTCT TTAACCTTCAATTTTCTCTGAATTTCTTGTTTCCATCTTCTTCAACCTTCTTCTTTAACCTGCTTCTTTAAAATGGATGA TATGAATCCATTTTATCAGTCTTCGGGTTTGATAGACTTATTGCATAGTCAGCAAGAAGACATCAATCGTTTTGCAAGTG GATCATCTGAAGTCCCAGCCTTTAATACACAAATGTTTGAAGAAGGAAGTAAAGATAAAGTAACACTCATGGGTTGTACA CAAGATGAAGTCAAACCGAAGGTTGGTTTATCACAAAAGAAATGGTTTCCCAAAGAAGATATTGTTCTTGTCAGGGCATG GCTTAACACCAACAAGGATCATGTGATTGGTAATGACCAACAATGTCAATCATTCTTGAAACAGATTGCATCATATGTTG ATATCAGTCCGCAACTTGATGATCTGCCAAAGAGAGAGCATGCTAAGTGTAAGCAAAGGTGGTCAAAGGTCAATAAAAGT GTGACCAAGTTTGTAGGTTGTTATAAGACGGCAACAACTCATAAAACTAGTGGACAAAGTGAGGATGATGTAATGAAGTT GGCTTATGAAATCTACTACAATGATACCAAGAAGAAGTTCAACTTAGAGCATACGTGGAGAAAGTTAAGGTATGATCAAA AATGGTGTGAAGCTGCAACTATTAAAGGGAATAAAAATGCTAAAAGAAAGTGTGTCGATGGTTATTGATATGGTTCAAAT TACCTAGAACTACGCTATCAAATAAGAGATCAATTGCAGTATTTTAGGATCGAATTCACGGAGTTCTTTTGTTCAAACAA TGAGTTA ................................................................................ ........................................................................i...N... ................................i...................................i........... ....N....................................i...................................N.. ....................N...........N.........i...............................N..... ..................N....................N........................................ ................................................................N..N....i....... .....N..............N..................................................i........ ...............................................................................N ....... Pos Score Pred ------------------------ 153 0.875 Yes 157 0.176 - 162 0.671 Yes 273 0.700 Yes 309 0.732 Yes 325 0.193 - 362 0.510 Yes 398 0.360 - 421 0.347 - 433 0.255 - 443 0.542 Yes 475 0.176 - 499 0.197 - 520 0.314 - 625 0.271 - 628 0.257 - 633 0.605 Yes 646 0.273 - 661 0.359 - 712 0.504 Yes 722 0.290 - 757 0.344 - 778 0.354 - 790 0.171 - 880 0.043 -

Explain the output -- Processing locus 1048: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAGCAAAAAGCTAGTAAAAAGGGATTATAATTGTATTGGGGTAAATAAAAACATTGGGGGCGAGATGCAAAATATAAAA GAGTAAGGGGTTTAAGTGTAATTTCGACATCGAGACCCGACCCGACCCGGGATTATGAATCCTGATCGCCGCGGGGTCCA AATAAATATATGTCAGCTCCTCCGTTCTTTTATTTTAGTCCGTCGGGAAATAATGGTAAGCGAACGGAGCTCCAAGGAGA AGAAGAGAAGCAGAGCTCGGAGCGAAGATTCTTCATCTTCCGATTACGAAGAGAAAGTTAAGAGGCATCGAGGAACAGAG AAAGATGATGAAAGGAGAAGTAGGAGAAGTGATAAGAAGGATAAGAAATCTCACAAGCACCACAAATCCAGTACATCAAA GAAGTCCAAGGATGATAAGCCGAAAAAGAAACATACGGAAAGCGACCACAAGCTAAAAGAGGGAATTCCAGAGCTATCAA TGGAAGACTACTTCTCGAAAAACAACGAGTTTGCTACATGGCTGAAAGAAGAAAAACGTACTTACTTTAATGATCTCACG ACCGAAGCTGCACGAGGACTGTTCTCACGATTTGTCAAGAGATGGAACAGAGGAAAACTCGAGTCTCGATACTACGAGGG AATCTCCACTGCCCCACGAACAGCTCACGACTGGATGATCAAGCGTAGGTGAAAAAAAGCAAAAGCAATACCTTCCTCTT CTGTGGTTCGCCGGTTACCTCTCTTCTTGCTTCTTGTACACGTGAAGGTTGATTGCATCAGAGATATATTT .................................................................N.............. ......................................................N......................... .........i..........................................i........................... ................................................................................ ....N..N........................................................................ ...........N...................................................................i .....................................i...............................i.......... .........................................N...................................... ..................................N............................................. ....................................................................... Pos Score Pred ------------------------ 66 0.308 - 135 0.329 - 170 0.534 Yes 213 0.755 Yes 325 0.384 - 328 0.248 - 412 0.251 - 480 0.598 Yes 518 0.611 Yes 550 0.563 Yes 602 0.399 - 675 0.315 -

Explain the output -- Processing locus 1049: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCGCGACTTTGAAAACTTCAATTTGGTGAAATTCTTTCAAATCCGCATCTTGTAAAAATCACATTTTTATATTTTTGTTG ATTGATCTTGTTCTCTGTTGAATGTTTAACTTGTGTGCGTTAGGTAGAACAGTAGAAGAAATCATGGATCCAGCTGAAAT GAGATATTTGGAAGAAGAGGATGGTCCGTTGATGAAGACAATCAAAGGTAGTATCACTGGTTTTGGTGCTGGGACTATTT ACGGAACCATTTTAGCCACATGGAAAGATGTTCCAAGAGTGGAGAGAAATGTGGCTCTTCCAGGACTTATTAGAACACTG AAGATGATGGGAACCCATGGGCTGACTTTTGCTGCTATAGGAGGTGTTTACATCGGTGTTGAACAGCTGGTTCAGAATTT TAGATCCAAGAGAGATTTTTACAATGGTGCTATTGGTGGTTTTGTCGCTGGAGCTTCTGTGCTTGGCTATAGAGCAAGGA GCATCCCGACAGCGATAGCTGCAGGTGCAACACTAGCTGTTACCTCTGCTTTGATTGATTCTGGAGGTCAGACCACAAGA GTAGACAATGGCAGAGAATACTATCCTTACACCGTCGAGAAAAGAGCTGAAGCTGATTCCTGATTTCTCTTTTGGTCACT GAGATTAATAAGATGTGCATTCGCTGATCCGGGGATCTTGAGATAATCTCTTTTGTTTATTTTATCTGGATATGTGATAT CA ................................................................................ .....................i.........................................i..............i. ....................N..........N................................................ ...................N.......N....................N............................... ...i..i.........N............................................................... .......................N........................................................ ................................................................................ .......i........................................................................ ............N..........................................................N........ .. Pos Score Pred ------------------------ 102 0.529 Yes 144 0.508 Yes 159 0.751 Yes 181 0.316 - 192 0.340 - 260 0.345 - 268 0.249 - 289 0.224 - 324 0.563 Yes 327 0.534 Yes 337 0.174 - 424 0.492 - 568 0.679 Yes 653 0.107 - 712 0.049 -

Explain the output -- Processing locus 1050: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 1051: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 1052: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATATTTGCTAGGTTGTCTTAGTAAAAGCATAGAGATATAATAGGAAACCGTCCAAACACTTTTGGGTTTGACAATAGT TGGCACAGTATCTAGTACTGATTTTGTCTCTTAATGACTACTAATAGTTGGCACAGTATCTTGGTTATTTACTTGGCTTC GTTGGCTCTTGATTGGAGCTTGGAAATGGGAATTCATCTTGAGAAGTCAGCGTATCTTGCGTTAGCTGGTAACTTTCTGA GATCAAACGAGCTCTCTAAAGTGATTGATGTGGTCAAGGAGATGGTGAAATCCCAACACTCGCTTGGTGTGTACCATGGA GCAATGCTGATCCACATGCTCGGGTTTGGGAGAAGACCTAGCTTAGCTGCAGAGGCTTTAGACTTACTTCCGGATGATCA AAAGGGTCTATCTGCTTACACTGCGCTGATGGATGTTTATATCTCAGCAGGGAGTCCAGAGAAAGCAATGAAGATATTAG GAGAGATGAGAGAGCGAGAGATAATGCCTTCGCTTGGGACATACGATGTTCTATTGTCTGGTCTTGAGAAAACCAGTGAT TTCCAGAGAGAAACATCGTCATTGAGGAAGGAGCAGAAGAGTCTTGTTGCTTCTACACGTTTCCGGGAAATTGTTCATGT TGAAGACAAAATATGCATGTGATCTTCTGTTTGCTTGCAAGATAGACAAAATCTGTTGCTTCTGTTTGATTACAAAATCT GTGATCTCAGAAACATTGATGCTTGTTGACCCTAAGCAAAG ................................................................................ .................................N.............................................. .........................i...................................................... ...........................N.............N.................................N.... ...i...........N.........................................................N...... ............................i...N..................................i............ .....i.................N.....................N.................................. ............................................................................N... ............i...N............................................................... ..................N...................... Pos Score Pred ------------------------ 114 0.469 - 186 0.722 Yes 268 0.268 - 282 0.401 - 316 0.163 - 324 0.712 Yes 336 0.439 - 394 0.161 - 429 0.501 Yes 433 0.110 - 468 0.787 Yes 486 0.699 Yes 504 0.488 - 526 0.144 - 637 0.188 - 653 0.501 Yes 657 0.318 - 739 0.050 -

Explain the output -- Processing locus 1053: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGTTCCTAACGGCACCGTTTTATTCTGGATTGAGTACTTTTCTTCTTCTAATAGATTTAATTGGGCTAATGGATCGGCC CACTTCGGCCCACGGGCATGAATATATATGATCCTAATAAAGCCAGCAAGGACACGGAGCCAAGTTTCATTCAATCTAAA GATGGGGAAAACGAAGCGATACTGGTTACTGAAAACAGAGCCAAATGAGTGGTCATGGTCAGACCAAGAATCAAACGGCG GTATCAGCAAATGGGACGGCGTCAAGAACAAACAAGCCCAGAAGAATCTTAAATCCATGACTTTAGGAGATCTCTGTTTC TTCTACCATTCAGGAACCAAATCAAGATGCGTCGTTGGCGTGGTTGAAGTATCACGTGAATGGTACACTGATGATGCTGA AGGAGTAGAAGGAGAAGGAGCGGTTGATGTCAAAGCGATTGGAGAAATGAGGAAATGTGTGGATTTGAAGGAAATGAAAG GAGACAAAGGGATTATTACTAAAGGTTTTGTTTTGTTTAGACAGCCGAGGTTGTCTGTTGTTCCTGTTGAGGAAGATGTT TGGAATATGATTTGTGAATTGGGAAATGGGTTTTGTGGAGATGGTAAGGAAGATTGTGAAAGTAGTGATGATGAATGAGT CTTAAATTGGATTCCGTTCTTGTATTATGTGATTTAAAGTTCTTAACTTTGTTAGGATTTTGGGGGTTTTGCTTAATGAT TGATTCGTAGTGGTGAT .....................................................................N.......... .................i.........i.................................................... ..........i.............................................i....................... ..........................N................................N..........N..N...... ..........................N...................N.......N..................i...... ...........................................................................N.... ......N..................N..............N..........................N..N...N..... ..........................N................................................N.... ................. Pos Score Pred ------------------------ 70 0.359 - 98 0.548 Yes 108 0.534 Yes 162 0.627 Yes 205 0.463 - 215 0.380 - 251 0.500 - 297 0.570 Yes 347 0.221 - 380 0.217 - 391 0.178 - 394 0.247 - 427 0.074 - 447 0.494 - 455 0.430 - 474 0.538 Yes 556 0.111 - 567 0.292 - 586 0.362 - 601 0.234 - 628 0.184 - 631 0.185 - 635 0.157 - 667 0.072 - 716 0.038 -

Explain the output -- Processing locus 1054: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACAGGTCTAAAACGCTGAGTGGTGGTGCGTTTTGGGCTCAAATTTTATCTAAATCCGTCGTCGTCTTATTAGAGACGTA TAACATTTGCGAACTTAATGATTATATTGGGCTTTAACTGGGCTTATACAGTTTCAGAAATCGAAGCCCATTATGTTATC AAGCATTGTTGTTGTTGTCACCAGGATCGAAAATTTTCAATGTTGTTGTTTGAGAGAGATGATGGCTGCGAAGCTTCAGA AATGGCGAAATCTGGCAGGTCGTCTAGATCTGATGAATCGGAGCGGCGCTGTGTCGACGAGGCGGTTCCTGCACGAAGGT CCAGATACCGTGGAGGAGCTTCTCGAAAGACATCTAGCGAAGAAAGAGAAACCAATAATCGATCACGATGAGGCTGAGTT TCTGAATAGACGGCGTCTGACGAGCACGCGCCGGGAAGCGTTGAGTTTGTACAGAGACATATTACGAGCGACTCGGTTCT TCACGTGGATTGATTCCAGGGGAAATTTATGGAGGGACGTGTTGAGAGAGAACGCGAGGAAGGAGTTTGAAGCGGCGCGA TTTGAGACGGATCCGGAGGTTATCACAAGGCTTCTGATAGGTGGAAGCGACGCCGTTTCGTCTGCTTTAGATAAGCTTGC GGAGAAGCAAAGAGAGATGATTGAGAAACAACGCCGTGGTGATCAACGCTGATTTGACGGATCATAGGGCATGAATGTTT CAACAGAATGATTCATCGAGTTTAAAGTGGTTGGTGTTTGAAATTCTTGCTGTAAAATGGTTGCCAGAAGA ................................................................................ .................N......................................................i....... .......................................i..................N..i.................. ...................................................................i............ ................................................................................ ............................i................................................... ................................................................................ ................N.....................................................N...N..... .......N................................................N.............. Pos Score Pred ------------------------ 98 0.390 - 153 0.531 Yes 200 0.762 Yes 219 0.281 - 222 0.642 Yes 242 0.837 Yes 273 0.575 Yes 388 0.540 Yes 509 0.569 Yes 657 0.461 - 711 0.163 - 715 0.101 - 728 0.078 - 777 0.114 -

Explain the output -- Processing locus 1055: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAAACGTCAAAAGATGTAAATTATGTTGATAACTAATAAGATATTGTTGGTGAGAGATAAATAAAAAAAGATGTTATTT TTACTATTAAAGAGTTACTAAATAATAATGTTAATGAACATTCGACACTATAAATACACGCATAAATCCTCATTGGTAAA ACAAAAACAAAGAAAATCAATTAAGAATAAAATATCAAAATATTTTAAAATGGAGAATAAAACAATGTTTATGATATTTT CTTTGATCATGGTTTTGCTATCGTTTTCTCATCCAACTTTCGGCAAAGAAAGCGATAATGACAAGCCACTCCTCATTTCA GACGACGAGTTTGATGCTATGATGGCAAGATCTCCTACATCGGACAATTATAACGAAAATGTTGGTAGTAAATATTCAAA GAAACAAATAAATTATCTTATGAACTGCAGTAAAAAGATGGCCGTCCCAGATAAATGTATAGAAGAAGTGATGGCTGAAA TTATTCAGAACAAAAGTGCTTCAAGAGATTGTTGTTTGGGGATAGTGAAAGCTGGAAAGGAATGTCACATGGAATATATG AAATTATTTTTTCAAATGTATGAACTCAGGCGTTTTACTTCTAAAAGGTTTTCCAAAACTAATGAGATATGGAACAGATG TTCCACCGAAATTGGAGTTGTTTCACCATATTCTGGTTAAAAAAATGTAACTAATTTTATTATTTGAACAATACTATTAT TTCCAATCGATTTTGTTTTATGTGATTTAGTTTTTTCTTTTGCCAACATGGAACCATTA ..............N........N...............................................N........ ...........................N.....N.............................................. .................................................i..............i.....N......... ........N................................................N...................... .............N....i..i....................................N..................... ...................i.................i................N...............N......... .............................................................N......i........N.. ...............N...N.........................................N......N........N.. ............................................N................................... ...................N...........................N........... Pos Score Pred ------------------------ 15 0.225 - 24 0.131 - 72 0.102 - 108 0.148 - 114 0.195 - 210 0.714 Yes 225 0.648 Yes 231 0.334 - 249 0.465 - 298 0.316 - 334 0.228 - 339 0.604 Yes 342 0.610 Yes 379 0.235 - 420 0.663 Yes 438 0.765 Yes 455 0.348 - 471 0.196 - 542 0.328 - 549 0.510 Yes 558 0.212 - 576 0.320 - 580 0.134 - 622 0.163 - 629 0.306 - 638 0.272 - 685 0.095 - 740 0.038 - 768 0.056 -

Explain the output -- Processing locus 1056: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTGGATTGTTTTTCAAATCATTAACATGGTACAAAAATTATTTAAATATATGTATATTTGAAATAATCACAATTTTTCA CAAAACACAACTCATTGCTAACCAGCTCATATGCTAAAAAAGACAACTCATTTCTCCACTCTTTTGACAAAAACAGAGCA CCAAACATTAAACCCTTCAAAAACACAAATCTCACCATCACACGTAAAAAAAGAAAATATGTCTATGCTTCTTCTTCTTC TTCTTCTTGTCCCATTCATTACTCCATCTTCGCAATCTTCCATTCGAAACCTCCTCGAAGCTCGCGGTTTACCAGGTGGT TTGTTTCCAGACAACGTGGAGAGTTATAGTCTAGATGATAAGACAGGAGAGCTCGAAGTTCAGCTACAAAATCCTTGTTT CGCTCGGTTCGAAAACAGAGTATATTTTGATCGTGTGATTAAAGCAAATTTAAGCTATGGTGGGCTTGTTGGACTTGAAG GTTTAACACAAGAAGAGCTTTTTCTTTGGTTACCTGTTAAAGGCATTGCAGTGAATGATCCTTCTTCTGGACTTGTTCTC TTCGATATCGGTGTTGCTCATAAACAAATCTCTCGTTCTCTCTTTGAAGATCCTCCTGTTTGTTATCCTCCTGGATCTAT AATGGAGAAGTTAGAAAAAAGCAAGATGGATATTCAGCTCAAGAGATGAATGAGTTTTTTTTTTTTTGGGGTTGTGTAAG ATTCAAAACTCAAACTTGTCTCTTTGAAACTTGAACAAAAAATGTGTTTTTTTCTTTCTTCTTTTACC ..........................i.......................N............................. ..............................N................................................. ..........................................................i.....i............... ................................................................................ ..................................N............................................. ........................................................N....................... ......................................................N......................... ................................................................................ .........................................N.......................... Pos Score Pred ------------------------ 27 0.525 Yes 51 0.335 - 111 0.413 - 219 0.750 Yes 225 0.505 Yes 355 0.146 - 457 0.181 - 535 0.210 - 642 0.620 Yes 666 0.287 - 686 0.436 - 690 0.158 - 762 0.081 -

Explain the output -- Processing locus 1057: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTCATGGTATTCCTTGTTTGTTGGATCCTCCATCTCCTTTCCCTCGTCATTGAGGCTTATTATAAATTATATGCAAGTGT TTTCTATGATAAACCTTCTAGATCCGTGTGTCTTCCGGTGACACTTCTATCGCCATAGTGCGACCTATTACTGCAGTATG CAGCCTTTTTCCTGAAGCTATTAGGTTTTCCTATGGCTCCTATGCCTCCTTTAGTTCCTTCTTTCATGTCTATATGATTT GTAGGTTCTCTAAAGTTGAGATGGGTAAATGTTCAGCTAACTCATGGAGTTGGAATTGTGATTCTCGCTTGTTCACAATT GCTTTTGGTCTTCACAAGGATACTAATTGTATGCTTTGTTTGTGGTTGTGCTTTGTTTGCCATTGTTACTCCTTTATGGA GATCTATGTTCTATCAAGTTCCCCTGTTGTTGGGAAATCTTTATTTGCAGGGAATGAAAGCTTCTTTGGTGTTTGTGGAT TTGCTGTTGCATCTTGTTTGGACATGCTATCTTCTAAAACCCTTGAAGTTCCCTTGCGAGACATTTCTGTCGCTTATCGT TCTAGCACCTACACGTTGGTAGCAGAAGATATTGTAGCGAAGACTGCCTTATCATTGAATAACGCCCTCTTGTTGGCGTG GAATGAAGACTCTTTGGTTTTGCAATTGTTCATGGACTCCCAGTTCAGTGTAAAATCTTTCAAGTTACTCGAAGTCACCG TTGAGCTAGGGAGAATTCTGCTTGTTTTAAGCTTCCTTCACCTCCGGTTTATCCCCTTGTTTTTTGCATTATTCTCTTAT AATGCGTTTGTATTTGCAGTTCTTTCTGTTTTTAGTGATGTGTTTTACTCTTGTAATGGAGACTAAATCATTTCTATATC TATGAAATCAGTTTGACTAAAAAAACAATAAGAACGAGGAACATCCTCGGGAAGAGACACACTCTGGAATCTCTCTGTTG AAAAA ...N..................................................................N......... .....N.......................................................................N.. ................................i........N.......................N.......N...... ....................N.......N..............N.................................... ..............................N............................................N.... .....N...............................................N.......................... .......................i........................................................ ................................................................................ ..N............................N................................................ ................................................................................ ..... Pos Score Pred ------------------------ 4 0.419 - 71 0.156 - 86 0.228 - 158 0.177 - 193 0.782 Yes 202 0.251 - 226 0.111 - 234 0.380 - 261 0.325 - 269 0.170 - 284 0.320 - 351 0.421 - 396 0.379 - 406 0.147 - 454 0.316 - 504 0.541 Yes 643 0.138 - 672 0.215 - 802 0.580 Yes 838 0.566 Yes 856 0.613 Yes 882 0.084 -

Explain the output -- Processing locus 1058: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAACTGTAAACTGATAAAGATAGCATTTTTAGATCTGCCTTATTAATAAAGGTTGGATTTTTATGATCAGAATCCTTGT ATCTATTGAACAGTGTTATAATATATGGTTTTATGTCATTACCCACGATGTTGCTTTAGTGGGGTAGTTGATGCGAAATT GGTCTACGAGCAAATGATTTCAGAAAGTGACATTTTTTATAAGCAAAAAAAAAAGCAAATCCACCAAACCCTTTGTCGTA AGAATATAATAATAAGCCGTTCGGTGAGTGCACTCTTTCTCTCTCACTGTTGTTGTTGTGTATCTGTGCGAGATCTCTCC AACGAAAAAATGGCTTGTAGACTCTTTTGGGCTTCTAGGGTTGCTTCTCATCTAAGGATCTCCGTTGCTCAACGAGGGTT TTCTTCCGTGGTTTTGAAGGATTTGAAATATGCTGATTCACATGAATGGGTGAAGATTGACGGGAATAAAGCAACCTTTG GTATAACGGATCACGCGCAGGACCATTTAGGAGATGTGGTCTATGTGGAGTTACCTGATGTAGGACATTCGGTGTCACAA GGGAAGAGTTTTGGAGCAGTTGAAAGTGTGAAAGCAACTAGCGATATCAATTCTCCAGTCTCGGGTAAGGTGGTTGAAGT CAATGAGGAGCTGACCGAGTCCCCTGGATTGGTGAACTCTAGCCCGTATGAACAAGGATGGATCATAAAAGTTGAGCTGA GTGATGCAGGCGAGGCAGAGAAGCTGATGGATTCAGACAAGTACTCTAAGTTCTGCGAAGAAGAAGACGCCAAGCACTGA GCTTTTTCTATTTTTTCCAGCATCCTTCTCCAAGCTTCCACCGCAACACAGATTGTTGTTTTCTCAATCGAGGATCAAAA CGAGATTTCTCCATCTTTG ...............................................................N................ ........................i.......N..............N......................N......... .............N.................................................................. ................................................................................ .........i...................................................................... .............................i...........N...i.................................. .................................N........N..............N...................... ................................................................................ ..N............................................N.........i...................... ...N......................i..................................................... ................................................................................ ................... Pos Score Pred ------------------------ 64 0.198 - 105 0.516 Yes 113 0.279 - 128 0.391 - 151 0.121 - 174 0.425 - 330 0.796 Yes 430 0.612 Yes 442 0.175 - 446 0.634 Yes 514 0.124 - 523 0.165 - 538 0.119 - 643 0.235 - 688 0.125 - 698 0.520 Yes 724 0.455 - 747 0.752 Yes

Explain the output -- Processing locus 1059: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGAGTGAATGAATGAATGTATCAATCATGAATTAGAGATTCCATTTTTTATGGCCTAAATCAATGTTTCTCACTTGTAAA AGTTGTCTTTTTGACAACTAATCATATTCTAATTCTTTTTCTCTGCCCTTTATGGAAAAGCTTTGTGACTATTTCTTTAT TTATTTCTGTCCAAATTTGATGATCTGTAAGAATCTCTTTATTGGGATTGCAATTTCAAACAAACTTTACATCTTCTTCT TCTCTCTGCTTTTGCCTTCACGACGAGTAGAGAGAGCCATGGACACACTCATTGCTCAGTTACAGAGACGATTTTGTGAT TTCACCATTAGTCTCTATCACCAGGGGTTTTTGGATGATCAATTCACAGAATTGAAAAAGCTGCAAGATGAATGTAGTCC TGATTTTGTGGCTGAGGTTGTTACTCTCTTCTTTGAAGACTGTGAGAAGCTTATCAGTAATATGGCTAGAGCTTTAGATC AGACAGGAAATGTAGATTTCAAACTGGTAGGTTCTAGTGTTCATCAACTCAAAGGTAGTAGCTCAAGTGTTGGTGCCAAG AGAGTCAAAGGTCTTTGTGTTACCCTCAAGGAGTGTTGCGACTCTCAGAACTATGAAGGGTGTGTGAGATGTTTGCAGCA AGTGGATATTGAGTACAAGACATTAAAAGCAAAGCTTCAAGATCTGTTCAATCTTGAGCAACAGATTGTCCAAGCTGGTG GTAGAATCCCTCAAGTGGATATATAAACAATAGACCTATCGACAACAGAACAGCAAGATGATGATCATCCAGTTGCATTC TTCAGACGAGTACTACTCGAATTTGTTGCTTCGAAAAAGAAAAAA .......N...N...N..........N......................N............N................. ...................................................N............................ ...................N............................................................ ......................................N......................................... ..................................N................................N...i........ .............................................................i.................. .........N...................................................................... ....................................................N...............N........... ................................................................................ .........................................................N..N................... ............................................. Pos Score Pred ------------------------ 8 0.089 - 12 0.293 - 16 0.442 - 27 0.313 - 50 0.199 - 63 0.201 - 132 0.136 - 180 0.417 - 279 0.461 - 355 0.131 - 388 0.251 - 392 0.501 Yes 462 0.755 Yes 490 0.280 - 613 0.300 - 629 0.253 - 778 0.054 - 781 0.037 -

Explain the output -- Processing locus 1060: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AACCTTTAAGAAGATTTTGTCAAATGTTGATAAATGAAATTTGCTTCTTTGTTAAATAAGAAACACTTTATGAGAAACAT TTAATTATGTACACTTTATAAATGTTGGAGCTTCGAACCCGTGACCTTGTAAGCAGAGCACTTCTGATTAAAATACAGAG AACGAAGACTGAGGAAAGAGAGAAGAAAATGACTCTGGGACTGATAAACGCGAATCCAGTGGTGCAAGCTAAGAAGGAAG GGCTTGTTCGTAGAGAAGATCAATATAGAGATGATGGTGTTGATCCTCTTGAAATATATGATTATGTAAGAGATATAAGA GATCCAGAACACCCTTATACTTTGGAGCAGCTAAGGGTTGTCTCTGAAGAATCTGTTACTGTCGATGACAAGCTCGATCG CATCCTGATAACGTTTACACCGACGATTCAGCATTGTAGTATGGCAAATATAATTGGTCTGTGTCTGAGAGCTAAGCTTA AAGAATGTTTGCAACTTCATTACAAGGTTGATATCAGAGTGTCTCCGGGTTCTCACGCTGATGAAGTTTCAGTTAACAAA CAACTGAATGATAAAGAAAGAGTGGTGGCTGCATTGGAGAATCCTAATCTCCGTCAGCTCGTGGATGAGTGTATCTACTC CGATGAGATATGATCACATCATTTACTTCTACTCTCTTGTACAGGCTTCATATCATGGGTTTATATAATGCCTTAGAGAG CGCTCTTTAATCTCAGAATACAAATTAAGGGT .......................N.........N...................................N.......... ......i..............i.......................................................... ............................i................................................... ..............................N..N.......................N.....N................ ................................................................N............... ........................................i....................................... ....N.......................................................N................... .......N........................................................N............... ..N......N............................................N............N............ ................................ Pos Score Pred ------------------------ 24 0.380 - 34 0.347 - 70 0.082 - 87 0.592 Yes 102 0.655 Yes 189 0.607 Yes 271 0.249 - 274 0.134 - 298 0.082 - 304 0.408 - 385 0.107 - 441 0.594 Yes 485 0.331 - 541 0.111 - 568 0.168 - 625 0.127 - 643 0.282 - 650 0.370 - 695 0.105 - 708 0.161 -

Explain the output -- Processing locus 1061: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGACAATGGTGGAGTTTCGAGTGTGAATGAAAAAACTCCATTTGCTCGAGAAGATTTGATGAAATGGTTTGTATAGTATT GATTTGATTCTACTTGAATGATTAATGTTTGAATTGCTCTTTGTTGCTTGCAGATTCCTGAAAAAAATGAATTTCTTAAA CTCAGCAGCATCCATTTGCAGAAGAGTTAGTTTGAGGGAACTGATCACTGAGGTTCCTGCTTATAGTGGCAGTAGCATTT CCGATGGTTCTTCAAGTGGCTTGAGTTTGGTCTTCAAGCGTTGGGCTACTAAGAAAACTGCTGGTTCTACAAAGAACGGT CGTGACTCTAATCCCAAGTTTCTCGGTGTTAAGAAATTCGGAGGAGAGAGTGTGATACCAGGAAACATCATAGTTCGTCA ACGGGGAACTCGGTTTCATCCTGGAGACTATGTGGGGATTGGTAAGGACCATACTCTGTTTGCATTGAAGGAAGGACGAG TCAGGTTCGAGAAAAGCAAGATTACAGGACGCAAATGGATTCATGTTGATCCAACAGGTGGTCATGTTCTTCACCCTATC TACACAAAAGCTGCAGCTGCTCAATCGACTAAGTTGAAGACAGCTTCATAGTCGTGAATACTGATTGCAACAATCTAAAC GTGGTTTTCATTTCCTTTAGATCATGTGATCTCTCGAGTAAAAGATGATGCTATCTTAGAGCAGAACCAC .....N....................N...............................N....N................ .................N......N.........................................N............. ................................................................................ ...N............................................................................ ................................................................................ .............................N.................................................. ..................................N.......N....................N................ ................................................................................ .......................N....................N..N...................... Pos Score Pred ------------------------ 6 0.181 - 27 0.145 - 59 0.149 - 64 0.421 - 98 0.487 - 105 0.347 - 147 0.466 - 244 0.241 - 430 0.243 - 515 0.223 - 523 0.231 - 544 0.177 - 664 0.046 - 685 0.059 - 688 0.046 -

Explain the output -- Processing locus 1062: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATATTTCGATATACAAAGCACTAAAATTCCAAAAAAATAATTTCTACTTCTTATTTGTGTATAATTATATTATTTAA GAAATTAAAATTGTATTGTAATTGCAAACTTTGATGTCTTATGTAATTTGTATATCATTTTTCTGTTTTGCAGTTATTTC GAGAAAAAATAGGCAAAACAAAATGAATGCAAACCCATGCGAAGTTTGCAGGTTCCAAAATAAACAATGCGTCAACAACT GCATGTTTGCTCTGCTATTTCCTTCAAGTGATCTAGAGAAATTTGATGTCGTCAATCGCATCTTTGGACTCGAAACTTTG ACATTCTACCTCAAAGACCTTTCACCTATGGAACGCATAGATACTACCCGCACACTCTATTACGAGGCAAAACCATGCTT TCTGAACCCACCCAAGAACCCCTCAAAATTCCTCGAAGCTCTTCTAAATTATCCTTACCAAAAAGCTGAAGAGGTGTCCA AAACAAAGAAGTTGCTCGCATCTTACTCGAGACCATGTGTAGTATTGGCTCTTCCTGCACCAAAATATACTCAGTCGAAA TCTAAACCATCTGTTTTGCGCAAAAGAAAAAGAAAGACTAAATCATCGGATGAATCGGCCATTCGTGTGGTTGAAGATTC CTAGATGGTCATTGTTGAAGATTTTTTTTTCTTTCTAAAAGTGAATCTTTGGAACCAAATATGCTTTTAATTTAATTTCA AATAAAATAAAATATGCTTTTAT ................................................................................ .................................N......N....................................... ......................N...N.........N.............................N............. ..N..........................................N.................................. ...........................N..............................................i..... ................................................................................ ..................................N............................................. .................................................N.............................. ....N.......................................................N................... .............N......... Pos Score Pred ------------------------ 114 0.217 - 121 0.216 - 183 0.337 - 187 0.341 - 197 0.315 - 227 0.390 - 243 0.379 - 286 0.447 - 348 0.415 - 395 0.767 Yes 515 0.368 - 610 0.353 - 645 0.229 - 701 0.074 - 734 0.090 -

Explain the output -- Processing locus 1063: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATTTGGTTTAAATGATTTCGGTTCAATTTAAAAAAATCAATTTGGTTCGGTTTGATATCGGTTATATGTTCACCATTCC TTGGGATTATTTAATAATTAAAAGAAAATGTATCGTCTTGCTTCTTCTTCCCACACTACTCCACCACTCCATAACAAGCT CTCACCTTTTCTCCTCCGCCGTAATCTACGCCACCAAAGTCAACGACTTCACCGTCGGAATTGTCAGATCAGAGCAATGA GAGCTGTAATCCAAAGAGTCTCATCTTCCTCTGTTACGGTGGATGGTCGAATCGTATCGGAGATTGGTCCTGGTCTCTTG GTACTAATTGGAATCCATGAATCAGATACCGAGTCTGACGCTGATTACATATGTCGAAAAGTTTTAAACATGAGATTATT CTCTAATGAAACCACTGGCAAAGGATGGGACCAAAATGTAATGCAGAGGAATTATGGAGTTTTACTTGTTAGTCAATTCA CATTATACGGATTCTTGAAAGGTAACAAACCAGATTTTCATGTCGCAATGCCACCTGATAAAGCGAAACCTTTTTACGCC TCTTTAGTTGAGAAATTCCAGAAAGCATATAATCCTGATGCTGTCAAAGATGGTGTGTTTGGAGCTATGATGCAGGTCAA TCTTGTAAACGATGGTCCGGTCACGATGCAGCTCGACTCACCTCAATCCACCAAGTAAGCCAAGATTGTTTTGCATAAAC TTATCAGGCATGGATTTGTTACTAAAGCAAGTCTTGATATGTTCCATAAGTGAGAAAAAGGCATCAACAGAATCATA ............N.....................................................N............. ...........................i.................................................... ............................................................................i... ..........................................N..................................... ................N.................................N..................i.......... .....N..................N..........N....i............N.......................... .......................................N.......i................................ .....................................N...........N................N..i.......... ...........N.............N...................................................... .........N............................N...................................... Pos Score Pred ------------------------ 13 0.296 - 67 0.168 - 108 0.523 Yes 237 0.733 Yes 283 0.255 - 337 0.486 - 371 0.131 - 390 0.701 Yes 406 0.256 - 425 0.265 - 436 0.466 - 441 0.608 Yes 454 0.226 - 520 0.091 - 528 0.784 Yes 598 0.223 - 610 0.157 - 627 0.436 - 630 0.523 Yes 652 0.302 - 666 0.319 - 730 0.060 - 759 0.030 -

Explain the output -- Processing locus 1064: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTATTTGTTTTTCAAATTACAATAGAACAGCAAAAAGTCTTTTTTTTTTTGTTCATAACATAGTAGCAACACCTTTGTT ATATACACTTATTTCCAATAAAAATTGATTCTTTTAAAGTTCAAGTCAAAAACAACAAATTTCTCTAGCAGCACTATCAT GGTGAAAACTATATATTATACAAGTAGCAATATTTATTATTACAAAACAAAAATAACTTTAGCAAGAAAACGCGGATCAG GCCCAAAAGCCCACGCATTATCCACGGAGTTTTGTTTACTCTCTCTTATCTTCTTCTTTTTTGTTTTCCTATTTATTCTC ACCAGACACATTCTCTTTCCCTCACTTGTCTTCTTTTTTCCTAATTCAAGAAACCATATAATCTTTTCCTCTGTATTTTT TTACAAATCCCTCAAAGAAACAAAATTTACAAGATCAAGAATGTATAACGAATGCAGCTCTCCCTCCACCGCAAGATCAA CAACGAGAGTCACAAATCACAGAATGAGATCTCCGATCTGTTGTATAGGAGCCAACGCGGTGGTTGAGCCAGAAGCCATG ATGATCGGAGGAGAACAAAGAACACCAAGATCTCCTTACGAATGGCTCAAATCTACGGCGCAGGAGCTAGAGTTAAGAGA TCGGTGCCGGCGTGTGAAGTCACGTATCAAGGTTACGTGTCGTAACAACAATTGTGCTTATAATAATTGTGTTCATCATC ATCATCATAGCCAGAGTTATCCTGGAGATTTCAGTTACGATCCGTTGAGTTACGCGCTCAATTTCGAGGATAATGTGAGA GCTGATGACGATGGTTCTTTTCCTAATTTCACGGCGAGGTTGCCTCAGTCGCCGGTGACCAAGACGAGATCGGCGACGGT TGATTTGATCTCTTTCTGACGGAATAATTAATTACAAAAAGATTCTTTAAAAAAATATCTTTTCGTTTTCTTGTGTGTGA CGGTGGAATGTAACTGTTCGCCGGAGAAATGACGTAAG ................................................................................ ..............................................................................i. ................................................................................ ................................................................................ ................................................................................ ........................................i..........N............................ .......................i.....................................................N.. N........................................N...................................... ................................................................................ ........................................................................N....... ....N.....N..................................................................... ................................................................................ .......N....................N......... Pos Score Pred ------------------------ 159 0.501 Yes 441 0.849 Yes 452 0.481 - 504 0.512 Yes 558 0.381 - 561 0.401 - 602 0.347 - 793 0.128 - 805 0.216 - 811 0.116 - 968 0.054 - 989 0.089 -

Explain the output -- Processing locus 1065: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATGTAACCCATCTCATAACAGATTTATAATTTCGTGTATAATATAATCTCCAAATCACTAATTATGACCTCACATTTT AGGATAATGTTTTTTTTTTAAATGCTTATGGTCAAGAAAGAAGATAACGATTCAAGCCACACGAAACAGAAGAAGACAAT GGCCTCTCTCTTCACACCATCACCAACCACCACTAGTAGTAGTGGAGACACCATCTATGTAGCGGCGGTGCCGCTTAAAG CCGCGGCCGGTCCGCCGCAACTTATTATGTCAATGGCTTACTCACTTAATATCTCAAATTTACAACATTTCATGGTCCTC ATCAAACCTTCTTCTCCAATCCGTCAAGAGGTTCTTGTGTTTGATTTTCAGCCACGTAACCCTGAAAGCATAGAAGCAGC AATCTCACTTTTGTCAGGAAATTTGATTCCAGGTGTAGTTTTGCAAAGAAGGTTGAAGAATGTTCCAAGACAAAGATGTT GGATGGTTGGACCATCTAAAGGGAATGATGCTATGGAAATGGCAATGGAGTTTAATAAGTCATGGGAGACTGATTTGAGA GTTGGATTTCATGATTGCCGACATTATACCAATGGTTTAGTGTTATACAAGCTTTAATTTAATCAAGAAGACTTTGGTTT TTATTTGTTTCTAAGTGTTTTTGGTTTTTAATCTTTTGGAATACAGAACTTGTCCAACATCTTACCGGAGAAATGC ..N..............................................................i.............. ......N..............N.....i..................................................i. ........................................................N....................... ..........................N.....N......................................i........ ................................................................................ ...........................................................N...............N.... ..N.....................N..N....N.....N.....i................N.................. ..........N....................N................................................ ........................................................................N... Pos Score Pred ------------------------ 3 0.122 - 66 0.783 Yes 87 0.233 - 102 0.423 - 108 0.577 Yes 159 0.872 Yes 217 0.198 - 267 0.157 - 273 0.450 - 312 0.626 Yes 460 0.326 - 476 0.291 - 483 0.467 - 505 0.109 - 508 0.265 - 513 0.421 - 519 0.356 - 525 0.509 Yes 542 0.143 - 571 0.100 - 592 0.173 - 713 0.047 -

Explain the output -- Processing locus 1066: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGATCTTCAAGGGAAATAAAAGTTTGGTGTGCAGCTGATGATTGATTGGTTCTGTGTAATTGTTAAATTGCATGATTAC GTAACTGAGAATTCGGTTTAACCGTTTAAATTCCGGTCTACTGAAATTGCTACGGAAATAAACCGAATTAAACCGAACCG GATCATGATTCAGTCTACTTTAGTTTTTGTGCTCCGTAGATTGAGAGAAGCCAATTTTAGGGAAAAGAGAGAGATGAGGA AGTTGAATCCGCTTCTGAAACGAGGCTTGAAGGCTATTGAAAATGGTGATTTGTTGAAGATCCTCCAAAGTGAAATTCGT CACGAAATCTCCCATCCTCGTTTCCAGGGCGTTGAGACCGGTTCTCTAGGAGATTTCAAATTGGATTGGGATTCACCGGA AAGCCAAGATATTGTTCTGAAGAGACAGTTTGATTCAGGCGAGAAGGTTGTGGTCTCTGCTTTATTGCAACCAGAACCTA TAGAACTAGAAGATGATTTAGTGTTTCCTAGGGAAGCTCATGCCAAAGTTTGTATCAAAAAGCCTGGTCTCAGCTCTATC TTGCAGTTTCATTGCCGGGTTTACGAATCAGGATCTGGATCTTCTCATTTCGACATCGAGAGCGCCTACTTTATCCGCTC TTTTGTTTCTGCTCCTTCTTCCACTTATGGAGACCATTTTTTCAGGTGAAGTAAGCTCATAAATGATAATCTCTCAACAT TTTGTTTAAGTTACTTGAATCATATCTGTTTTACTTCATCCAAACCTCAAAGCATTGGGTACACTTCC ......................................i.................................N....... ................................................................................ ....N....................................................................N...... ..........................................i..................................... ................................................................................ ................................................................................ ............N..........................N........................................ ................................................................................ ..........................N...................................N................. .................................................................... Pos Score Pred ------------------------ 39 0.554 Yes 73 0.168 - 165 0.311 - 234 0.411 - 283 0.505 Yes 493 0.401 - 520 0.299 - 667 0.234 - 703 0.107 -

Explain the output -- Processing locus 1067: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAACAAAACTATTAAATGTTGAGGTTTATGATATCAGTAACATAAAACTATTAATGAATAAATGTTTTATCAATTAATTA CCATTATCGATTGTCCTATAACATCTCTTCTTTTCTTGTTTCATGTCACCAATCTTACATCTAAAACTAAAAACAAATAT GGCAAAGTCTTTTTCCATGGTAATGCTTGTATCTATAATAATATTTTACATGGCTCGTCCAATTTACTCTCGGAATAAAA TATTAGAAGATATAGACATATCTCCAACTCCAGTGGAAGGAACTGATTCACCCACAACAGAATATGAGATAGAGCTTGCT CACCGCTTACATGAAGATTATATTCTGGCTTGCCCTAAAAAGCCGAGTTCCAAATGCGATGATGAGATTTTCAATAACAT GTTGGATGGGACGACGCCAGTGACAAATGAATGTTGTCATGATATATTGAAGACTGGCAAAGATTGTCACCTTGCATTGG TTAAAATCATATTCTCCACGAATGATTATAAAAAAATTGCATCTAAGGCTATTCCAAAAAGTAAACAGACATGGAATGAT TGTGTCCGTAGAGTTGGGATGGAGATTGGTGCTCCGGTCTCTTTCGAACCTTAAACCAATACAATAATATTCCCTCATGC AGATATGTTTATGCGTTTTTTTAATTTGATTGTGATCATTATAAAACATCATGCATTTTGTTTTTGGTAATCA ...............N...........N.........................N.......i.................. ..........................................N...................................N. ................N.....N..........................N.............................. ...............................................................N................ ..........N..........................................N....i..N................N. .....N....................N...N.......N......................................... .....................N................................................N....N.... ..................N.........................................................N... ....N.....N.......................................N...................... Pos Score Pred ------------------------ 16 0.254 - 28 0.141 - 54 0.182 - 62 0.616 Yes 123 0.087 - 159 0.458 - 177 0.375 - 183 0.443 - 210 0.441 - 304 0.257 - 331 0.124 - 374 0.381 - 379 0.530 Yes 382 0.138 - 399 0.454 - 406 0.120 - 427 0.137 - 431 0.238 - 439 0.104 - 502 0.190 - 551 0.409 - 556 0.264 - 579 0.239 - 637 0.066 - 645 0.077 - 651 0.065 - 691 0.077 -

Explain the output -- Processing locus 1068: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GACACTCTTCTCCATTGCCTAACACGCCTCGTCGTTCGACACATGTCTCGTTTAAGTTTAGCCTCTCCTTCATACTAGTC TATTTTCATTCTTCATAATAATCACAAATGGTAAGCTTATTAAAGCTACAAAAACAACATAGAACATTAAATCCATACTC TCTTAGAAAAAGAAAAAAAGAAATGGCGGATCATCAACAACATCAGCAACAACAACAACCAATAATGAGGAGTCTCCATG AATCATCACCATCGACTCGGCAGATAGTGAGATTCGTAACGGCAGCTACGATCGGCCTATCACTCCTCGTGCTCTCAGGA CTAACACTAACCGGAACGGTGATCGGTTTGATCGTAGCGACGCCGTTGATGGTTCTGTTCAGCCCGGTGTTGGTACCGGC AGTGATAACGATAGGGCTTTTGACGATGGGATTCCTATTCTCCGGTGGTTGTGGGGTGGCAGCAGCTACGGCGTTAACGT GGATTTATAAGTACGTTACCGGAAAACACCCGATGGGAGCGGATAAGGTGGATTACGCGAGGATGAGGATAGCGGAGAAA GCCAAAGAGTTGGGACATTATACGCACTCGCAGCCACAACAAACACACCAAACCACAACAACTACTCATTAGAGTTTAAT AATTATATGGATCTATATGTTGTATTAAGTTTTATGATTATCCTACGTATGTTTTTTTAATACTGTTCTTACCTTTTTGA AAAGATCTATC ..........................................N..................................... ...........................N.................................................... ......................i.........................................i............N.. ................................................................................ ................................................N............................... .........................N...................................................... ................................i.............................N................. ................................................................................ ......N.........N................N..............N............................... ........... Pos Score Pred ------------------------ 43 0.469 - 108 0.445 - 183 0.702 Yes 225 0.669 Yes 238 0.428 - 369 0.295 - 426 0.435 - 513 0.725 Yes 543 0.153 - 647 0.097 - 657 0.082 - 674 0.057 - 689 0.030 -

Explain the output -- Processing locus 1069: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATTTAAATAAATAACTTAATTATTATAAATTGATTAAAATAATATTGTTGAGAAACCCACGCAGTATTAGCCTGATGG AGATGGTCTTAATTTATATAGCTATTCGAGGGAGAACAAAAAACAGTAGGCACGTGCCTACTTTCCCCATGCTGTACGTC CGCATCTGCCAGTATGCGTTCATTGGCCTACGAACTATGTCTACTATATATAGAGAAGCATGCGTTCTTCTTCCAAACAA CAAAACCAAAATATATATTTTTTCTCGGAAAAAAAAACATGTTTTCAAAGATGCAGCTGGTGGTTCTCATGTCGTTCTTG CTCCTTCTTCCATTGTGTTCTTCAAGGTTTGGAGAGAGCCATGAAGATGGAGTTCCTCACACCGACCAATATTCACTAAA TATGGTTGAAGAGAGTATTCCGAAAATGATGGAAACAGGCACAAACCCAAGCCACGATCAGACAACAAACGGTTGGGGTC GTCCTACGCGGCCACCGCCATCGCGGATGATGGATTATCCGAAAACATACCCAAACCCAAGCCGCGGTCAGACAACAAAC CGTGTTATTCGTTTGTCGCCACCGCCACCACGGATGATGGATTATCCGAAAAAAGACCCAAACCCAAGCCACGATCAGAC AATAAACCGTGTTATTCGTTTGTCGCCACCGCCACCACGGATGATGGATTATCCGAAAGCATACCCAAACCCAAGCCGCG GTTAGACAACAAACCGTGTTATTCGTTTGTCGCCCCGCCACCGCGGATGATGGGTTATCCGAAAACATACCCAAACCCAA GCCACGATCAGACAATAAACCGTG ............................................................................i... ..N.................................................................N........... .............N......................N......................N.................... ......................................i...........i.................N........... ........................................N.....N................................. ..........................N..i.................................................. .................................N..i........................................... ........................................N..i.................................... ..............................................N..N.............................. ........................ Pos Score Pred ------------------------ 77 0.526 Yes 83 0.173 - 149 0.254 - 174 0.242 - 197 0.499 - 220 0.203 - 279 0.768 Yes 291 0.602 Yes 309 0.217 - 361 0.449 - 367 0.456 - 402 0.850 Yes 426 0.695 Yes 429 0.392 - 507 0.354 - 510 0.544 Yes 594 0.411 - 597 0.659 Yes 681 0.440 - 684 0.647 Yes 767 0.118 - 770 0.137 -

Explain the output -- Processing locus 1070: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 1071: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ACCACACAACATTTTCAACTTCTTCTTCTCTTCCGTTTCACCACCCTTTCTTCTCCTAAATTCGAAGAACCAAAACCAAA AACGTGAATTCGGAGTTATGATTTTGGATCTTCTTCCCGTCTTTATATATAATTTTCTGGGTTATGTTTTTCTTCTTCTT AATTCATCTGACCATTGGATTGGTTTTCGATTGCAGAGTCTTCAATTGGAATTTGGATCGTGGGATTTTGGAAATGGCAT CGAAACGGATTTTGAAGGAATTGAAGGATCTGCAGAAGGATCCTCCTACTTCATGTAGCGCAGGACCCGTTGCGGAAGAC ATGTTTCATTGGCAGGCCACTATAATGGGTCCATCGGATAGCCCTTATTCTGGAGGAGTTTTTCTTGTAACCATCCATTT CCCTCCAGATTACCCATTTAAGCCTCCTAAGGTGGCTTTTAGGACGAAGGTGTTCCATCCAAACATTAACAGCAATGGAA GCATCTGCCTCGACATCTTGAAGGAGCAGTGGAGTCCTGCTCTCACAATTTCCAAGGTGCTGCTATCGATCTGTTCTTTG TTAACGGATCCAAACCCAGATGATCCTTTGGTCCCTGAGATAGCTCACATGTACAAGACAGACAAGAACAAGTACGAGTC CACTGCTCGGACCTGGACCCAAAAGTATGCCATGGGCTGACACAAATACTGTCCTTAAGGAAGAGCCCTAATAATTAAAC TCTTCTTATTTCTATGTAATGATCTTTTATAGACTTGTCTGTCTTATAAAATTTGTGAA ................................................................................ .................N.............................................N................ .........................................................................i...... ....................................................N........................... N.......................i....................................................... ..........................................................................N..... ................................................................................ ...................N............................N............................... ..........................N....N................................................ .............N....N........................................ Pos Score Pred ------------------------ 98 0.418 - 144 0.350 - 234 0.805 Yes 293 0.183 - 321 0.402 - 345 0.796 Yes 475 0.342 - 580 0.432 - 609 0.353 - 667 0.437 - 672 0.386 - 734 0.043 - 739 0.052 -

Explain the output -- Processing locus 1072: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGATATGATTGATTACTAGTTTGGGACCTCTTTAGACAAGAACTACAAGAGCATGTGTTTCTGTCTTCTTTGGTTGCTCT TAATTATATCTAAAACTCTAATACTTTCTCTTTATATATAAAATGCAACAAAGCTTAGAGCATTCTTCATCATTCACCAC TTTCATTACTTCAGAGATTTATACTACAACACAACTTACAATCCCTTTTTTGTTTGCTCTCTTTCTATTAGTAAAGATGA TCAACACTAAGAAACTACTCAAGATGGCCAAGAAATGGCAACAAAGAGCTGCCCTCAAGAGGAAAAGAATCTCATTCCAA AGATCAACTACTACTACTACTACTACTACTACTACTACTAGCAGCTCAACTGCTGTGGAGAAGGGCTGTTTCGTGGTTTA CACGGTTGATAAAATCCGCTTTGCTTTTCCGTTAAGTTACTTGAACAACTCTGTTTTCGAAGAGCTCTTGAAGATATCTG AAGAAGAGTTCGGCCTCAGGGCGGGTGGACCAATCACGTTGCCGTTCGACTCGGTTTTCTTGGAGTATCTCATCAAATTT ATCGAACGAAGAATGGATGGAGATACAGAAAAAGCTCTTTTAATGTCAATCTCTAGTGCTAGATGCTCTATGCAACCACA AGAACAACAGAGTGGTTATACCCAACAATTACTTGTATTTTAGAGAATTAGAGTGTAGACATTTTTTCCTCTGTAAAAAA TTATACTGTATTAGATAGACAGATCATTTCAAAAATACAAACTACTATATATTTGTCTCTCT ....N...............................................N........................... ..........................................N..................................... ............................................................................i... .......................i..........i............................................. ................................................................................ ................................................................................ ................................................................................ ............i...N.........................N...................N......N.......... ................................................................................ .............................................................. Pos Score Pred ------------------------ 5 0.225 - 53 0.316 - 123 0.195 - 237 0.689 Yes 264 0.575 Yes 275 0.549 Yes 573 0.589 Yes 577 0.397 - 603 0.410 - 623 0.252 - 630 0.092 -

Explain the output -- Processing locus 1073: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTTCTAATCTTTTTTGTACTTAACGCAAAACATTTTGGAAAACAAAGCTTTGTTACTTATTTGACGTGAAGTGAAGTT TACTTTTCTTATTAAAATTGAATGATTAATCTTCACATGGGCTTAGGAAGCCCATATAATTGGACCATTGATCTTAAGCA AAAACGACGACGTATTATTGTTTCTCTGATTCTCTCTTTCTCTAAATTATCAAAAAAACGCTACACAGTCGTCGTCAAGA ACAAAGACGATTCGATTGAATTCTCCATAAGAGGTCCGACCCGAATTCGAAAACCCGTAAGAACCGAATCGAAGATGAGC AAGGATGGTTTGAGCAACGACCAAGTCTCATCAATGAAGGAAGCATTCATGCTATTCGACACAGACGGCGACGGCAAAAT CGCACCGTCGGAGCTCGGAATCTTAATGCGGTCACTCGGCGGCAATCCAACAGAATCACAACTCAAATCAATCATAACGA CGGAGAATCTCTCGTCTCCGTTCGATTTCAATCGATTCCTAGATCTAATGGCGAAACATCTGAAAACAGAGCCGTTCGAT CGCCAGCTTCGTGATGCGTTCAAAGTGCTTGACAAGGAAGGTACTGGATTCGTTGCTGTGGCAGATCTGAGACATATACT TACGAGTATCGGTGAGAAATTGCAGCCGAGTGAGTTTGATGAGTGGATCAAAGAGGTTGATGTTGGATCTGATGGTAAGA TCCGGTATGAGGATTTTATTGCTAGAATGGTTGCTAAGTAAGATCTAAATCTGTGAAAATTTGAATTCTTTAGATTAGAA CAAATCTTAGGATTTGTTCAGAAAGTGTTTAATTGTTAATGTAATTGGGAATTTGGTCAT ................................................................................ .....................N..............N........................................... ................................................................................ ..........................................................................i..... ....i............................i..............N............................... .........................N...................................................... ...............................................i................................ .............N.................................................................. ......................................N....................N...........N........ ......N...................N..................................................... ......................................N..................... Pos Score Pred ------------------------ 102 0.168 - 117 0.403 - 315 0.676 Yes 325 0.651 Yes 354 0.507 Yes 369 0.340 - 426 0.312 - 528 0.796 Yes 574 0.199 - 679 0.109 - 700 0.213 - 712 0.140 - 727 0.049 - 747 0.248 - 839 0.051 -

Explain the output -- Processing locus 1074: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATAAAATAATTTTCGCTTCCTTAGTTTACCAACAACAAAATTACGCTTCCTGTTACTTGTTAAATGAAAATAAATACTGT TCTTACCAAACACAAAAATGAAAATACATATTGTATAATACACTTAGACAATACTTAAAATATGAATTAACAACACGTGT TATGCTTATCAAGAAACTGCTACGCATAAGGCACCTACCTTGCCTACCTCTATTTTTGTTTTCTTCTCTTGGTTTTCTAC TCTATGCTTTTTGTTATGGTAAAGATAAACAACAGAAAAGTCAGCGTGAGAGACTGGATTATCTTAGAGCCATGGCTGGT GTCCAGACTCTCGAATTAGAGATCCAAGTGAATATGACCGCCGAGAGATTCTTCAAGACTTTCAAGAAGAAAGAAGGAAA TTTCACGGACAAGACAGAGGCAGTTTATGTTCATCGTGACGATCCCACATCCAACTCCTCTATTCAGATCTGGAATTTCA TCGTTGATGGAAAAATGGAGCAGATCAAAGAGAAGATAGACGTAGATGAAGAGAACAAGTCGGTGTCTTTCTTAGCTCTT GAAGGAGATGTGTTAAAGCAATATAAGAGCTATAAGATAACACTTGACGTTGTTCCTAAGGATCATAAAGTTTGTATTGC AAAGTGGACATGGGAATACGAGAAGTTAAATGACGACGTTCCTCCCCCTACCAGATACACTGCGTTCGTTGAAGATTACA CACGCGACCTCGAGACTAGATTGTTGTCTGAATCATGAATATATCATCAATGTCTCCGAGTTCCTTGTCAAATAAATCAG ATCTTGCTATGAAAATAATATTTTTATAAATTTTCATAAATGATGACATGAAACAAA ...............................................................N................ .................N...........................................N.................. ...N...........N.......................................................i........ .................................i.............................................. ..........................N..................................................... ......N.......i..............................N.................................. .......N........................................................................ .........N...................N.................................................. ..................................i..............N.............................. ........N..............................N..N....N......... Pos Score Pred ------------------------ 64 0.243 - 98 0.499 - 142 0.429 - 162 0.240 - 244 0.195 - 256 0.230 - 312 0.762 Yes 354 0.764 Yes 427 0.133 - 487 0.061 - 495 0.750 Yes 526 0.138 - 568 0.147 - 650 0.267 - 670 0.431 - 755 0.513 Yes 770 0.255 - 809 0.050 - 840 0.078 - 843 0.078 - 848 0.035 -

Explain the output -- Processing locus 1075: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATAAAGAAAAGTGAAATGTTTTGGGTCATATTCATCATAAAATGCAAATAGCGGGGCGAAAAGTATGAAGATGGGAAAA AACGAAAAGGACTGAAAGTGAAAGGGCTTAACCAAAAGCAAAATAATGGGTCCCACAACACGTGGCCTGTGTCATTTCTT CTTCTTCTCCATCCATATAAAGAAGAAGAAAGAGATGAGATGCAAGGTAGTATATGCAGCAAGAACAACAACAAGAACCA AAGTAACAAAAACAATCTCTAAAACCATATCAATGGGACTACAAGGTCAGCTCTCCGACGTGTCATCAGATTCGATCCCA CTGATGCTACTGGCTCTCCTCGCAACTTTCTTCAGACACGTCCGGTCTCTTCTCCTCTTCCCTTCTTCTGCCCCCGTTGT TGTTGTTACTTCAAACCTCAGCGTCCTCGCCGACCAGCTCAACCTAAATCGCCTCTTCTCGTACCGCTACTCCGACAACG CAGCCTCTGACTGCATCGTGTGTCTGTCTAAACTCAAGACCGGAGAAGAAGTGAGGAAGCTAGATTGCAGACACGTCTTC CATAAGCAGTGTTTGGAAGGCTGGCTTCAACATCTCAACTTCAATTGCCCGCTCTGTAGATCTCCATTGCTACCTCATCA TCATCAGGGACATGGCAGTGATGCGTCGATCTCAGCCTTCCCTCTTCGCTCTACCTCTACTGCATCATCTCATTGATTTT TATGTGAAGAAAGAAGAAAGAAGAAAGCAAGAGAGAAGAAGATGGAGGGAGGATGCTTTTTTATTTATCTTCCTTCTCCT CTTTCCTTTTTAGGA ................i.........................N......................N.....N........ .............................................i.................................. ..................................N....i.............N.......................... ................................N............................................... ...i............................................................................ ................................................................................ ................................................................................ ................................................................................ ...........i........i........................................................... ............... Pos Score Pred ------------------------ 17 0.796 Yes 43 0.347 - 66 0.387 - 72 0.337 - 126 0.789 Yes 195 0.372 - 200 0.777 Yes 214 0.262 - 273 0.391 - 324 0.505 Yes 652 0.757 Yes 661 0.662 Yes 722 0.082 - 762 0.101 - 773 0.060 -

Explain the output -- Processing locus 1076: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGAAACAAAATAGAAAAAAGGAAACTTTGTAACTTTTAATCCTGACCGCTGAAATTTTGCTGATATCTTGACCGTCCTTT GAATTGATAGCTCGTCTATAAATAATGCTCTTGCTCTTCCTCTCTATGCTAAAATCTCGAAACCCTAATTATTCTAAGAT TCTAATTTGTGAAGTTTGTGAGGTTTTAGGGATGGGTTACAATTTCTACATGAGAGTGTATGAGGTTGTTGATGATGCTT CAACGGATGCAATCATATCGTGGAGCGAAAGCAACAACAGTTTCATTATCTGGAATGTTGGAGAGTTTTACAGAAGGATT CTGCCTAAATATGTAGACTTGGGCACAAACCTCTCACGGTTTTTCTCCAACCTTCGTTCTCATGGGTTCAAAATAGTTAA AGGAAGAACTGGAGTATTGGAATTTGGACATGAAGATTTTATCAGAGATAAACTGGAGCTTATGAAGAAGATGGTTAGCG ATAAACGTAAAGCAAGGAAAGCTGCTAAATCCAAAGCAAGGAAAGCTAGAGTTCAAGTCGAGTTTCTCTTCCAACACTTG CAAATTTGATCGTCCATTAAATCAGTCTTCTTTATTATGTATATTCGTCTCCAAGTAGATTCAAGTCTAAGAACTTTTCA ATAGTATGTTTCTCTCTTTATAGGAACC ................................................................................ ........................N....................N.................................. ...............................i.................N.........N...........N..N..... ......N...............................................N......................... ..........N..................................................N.................. .............................N...............................N........N......... ................................................................................ ....................................N........................................... .....N...................... Pos Score Pred ------------------------ 105 0.210 - 126 0.210 - 192 0.570 Yes 210 0.315 - 220 0.162 - 232 0.145 - 235 0.081 - 247 0.206 - 295 0.312 - 331 0.363 - 382 0.184 - 430 0.436 - 462 0.267 - 471 0.398 - 597 0.076 - 646 0.055 -

Explain the output -- Processing locus 1077: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTAATTCCGGTTTTTAAAATTGTGAATTTTTTTTTGTTTTATTGATATGTCGGTGCTATAATAGTTCTATGACGTGGCC ACAAAAGAGACACACTCGTAAGACATGTGTTCAAATCTTGTCCTCATGCAAGGATCATATGAGCCGAAATATTCTCTTAT ACTCGAAAACCCAATCTTCACATTCTCGTACAATCATCATCTACACCCAAAAAGAAAAAGGAAAAAAAAATCAATGGCTG AGGAGTTTCATCTTCCTCTAAACCTCACCGAACCAGACCAAACTCCGACCGAAGAAGATTTCGAAGCAGACACTACATTA ATTGACGACGAATACCTCCGTTACCTCCACTACTTGGATTCACGGAGCAATCATGGAGTCAACACGTTTGGATCTTACGA TGAAATCTTTGGTCTAGTTCTCGGTAATTCATCCTCTTGTACGCGGTGGCTTAACGCAGGGGAGGAACTTCCGGTGGTGG AGTTTACGGCTGAGGAAATGATGGAGAGAGGTTTAGTGGTTTGTGCTATATGTAGAGAGGAGCTTGCTGCTAATGAGAGG CTCTCAGAGCTTCCTTGTCGGCATTATTACCATAAAGAGTGTATCTCGAATTGGTTGAGTAATCGGAACACTTGTCCGCT TTGTCGTCATAACGTGGAATTACCCAACCACGGTTGAGATTGTGCATGTAGTATTTTCTTATTGTTCCGACCAAACTGTA TCCGGAGCCTAAGAGTTTTATTTTTGGTAAATATTATTGTTAATTAGTAACTACTG ...............................................i.....................N.......... ........................N....................N............N..................... .........................................................................i...... ................................................................................ ....................................................i..........................N ................................................................................ .................i..N............................N......................i....... ................................................................................ .............................................N.................................. ........................................................ Pos Score Pred ------------------------ 48 0.776 Yes 70 0.469 - 105 0.387 - 126 0.223 - 139 0.341 - 234 0.922 Yes 373 0.627 Yes 400 0.317 - 498 0.609 Yes 501 0.365 - 530 0.106 - 553 0.601 Yes 686 0.110 -

Explain the output -- Processing locus 1078: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCAAGCGAGTTTTAATATTTCAATAATTTTCATGTATATAGAAATTTTGCATTGCTAGTTAAAATAATAACAAATAGAT GTTGTCAAAACTCGAAAATAAAAGGGGTGTTTCTGTAGTTATCATAATTTAAGGAATTATATGATAGAAAAACAAATTTG GGCGGTAAATTTGAAAAAAATAACTTTTTCCAGTCTCCCCAAAAGTACCCGATTTGCCAAATTTGACCCTCCCATTTTAG CTTTCTGTCTATTCACCAGATTCTCTCTATTCGATTCTCCCTTTACTTCTTCTCGTCTCTCTTCTCTGCTCGTCAGATCT CAAAATCAATCGGATATCGAAACCACAACTTCATCAATTATGACTCTTGGCTCAGGAGGATCTAGCGTTGTTGTTCCGAG GAATTTTCGGTTGTTGGAGGAGCTTGAACGTGGGGAGAAAGGTATTGGGGATGGAACTGTGAGCTATGGAATGGATGATG GAGATGACATCTATATGCGTTCTTGGACTGGCACCATCATCGGTCCTCACAACACTGTTCATGAAGGTAGAATCTATCAG TTGAAGCTCTTTTGTGACAAAGATTACCCGGAGAAACCTCCAACTGTCCGATTCCATTCACGTGTCAACATGGCTTGTGT CAACCACGAAACAGGAGTGGTGGATCCTAAGAAATTTGGGCTACTTGCAAACTGGCAAAGAGAGTACACAATGGAGGATA TACTGGTACAGTTGAAGAAGGAAATGTCTACGTCGCATAACCGTAAGCTTGTTCAACCTCCGGAAGGTACTTGCTTCTAG TTACTTCTGGAATCTTCTTCTGGTTTGCTAGAAAATCATTGCCCCTTCTAATACCAAAGTATGTATCTGGGGGTCCTCCA TTGTCGTTCTCAACGCTTT ................................N.............................................N. ............................................................N................... ................................................................................ ................................................................................ .......................................i........................................ ..................................................N..............N....N...N..N.. ...N..........N.............................................N................... .....................................................................i.......... ......................................................................i......... .......................N........................................................ ............................................................N................... ................... Pos Score Pred ------------------------ 33 0.211 - 79 0.175 - 141 0.455 - 360 0.865 Yes 451 0.137 - 466 0.376 - 471 0.458 - 475 0.071 - 478 0.444 - 484 0.156 - 495 0.424 - 541 0.486 - 630 0.734 Yes 711 0.513 Yes 744 0.473 - 861 0.088 -

Explain the output -- Processing locus 1079: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTATAATCGTGGAATTTGTAATCTATAGCTTAATTACGTTCAAGTAGATTCGAGATTTGATGTGATTTTGAAGAATTGA TTAGCTGAAAATGTGGAGTGATCTACGTTGTCTGTGATGATGATATTGATGGTTTTCTTCTTCTTCGGCTTTCTTCGGTT CTGCAGAGTGTATACAAAGGTTGTTGTGGAGCTTTTATCAGATCACAGGAATAAGATGGAGGTATTTGGAAAATCTCCCA TGGTTGTTACATCTGCCAATGTTATTTACTTGTCTGCAATTCTGGGTCGCGATTGCGATGGACACAAATGTGATTGGAAA TGTGAGAATGAAAATGTTTGTGGGAATATGTATCGCTGCAAACTAACTGGACTAACTCATGTCTGCGACAAGAACTGTAA CCAGCGGATTCTCTATGATAACCATAGCTCTTTGTGCCGAGCCAGTGGGAGGATTTTCCCTCTCTCGTCCGTCGAGGAGC AGGCGGTTAAAGGTGTTCGGAGGAAGCTTGACGCCGCTGAGAGTCATCCTTCTGAGAGCTGTTCGTTTAAGCGTCGTCGT CGTGATGCTCAGTTCCATGCTTCTCCTTTCGAGAGGTCTTTTACTGCTGTGAGCCCTATCTGCAGCCCGGCTGGAGACGG AATGGAGTTGGTTTAGATTCCATGTTGATCTCCGCAAATCCTGAGACTGAGAGGCCTCTTTATCTTACGTAGAATAACTT CCCTTTTCTCTTTCTTTTGGACAACTATGGATATG ............................................................N................... ..........N.........................N..N........N............................... .......................................................i.......................i ..................i......................................N.........N...........N .......N.....N.............i..............................i..................... ..............N................................................................. ................................................................................ ....N...........N............................................................... ..........................N.....N.. Pos Score Pred ------------------------ 61 0.064 - 91 0.309 - 117 0.159 - 120 0.274 - 129 0.342 - 216 0.584 Yes 240 0.589 Yes 259 0.505 Yes 298 0.196 - 308 0.363 - 320 0.332 - 328 0.230 - 334 0.461 - 348 0.608 Yes 379 0.711 Yes 415 0.205 - 565 0.153 - 577 0.194 - 642 0.513 Yes 662 0.237 - 747 0.062 - 753 0.037 -

Explain the output -- Processing locus 1080: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GTGGATCAAACGCTGTTATAATCACGAAGGCGATGAAGAATGAGAGATTTCACATCACGCGTCCATGGCGATTCATAAGA ATTTGAAATCGGAGAAGATGAGAAATGAATTTTGGCGGCAAAATCAATCAACCCTTATTACAGATTCTTTGCTCTAATTA TATATTTCATTTTCTAAGGTTGCCACGTCATCACATTATCCAGACGATACTGATATGTGATCACACGTGTCATATCACAA GTGGTTTAAAACGATTCCTTTTAACCCCAAACAATCTAAAGCTTTGTCCACCTTTTCGGATCACTCACACAACTTCCAAC GATCACACACAAATGGCGATGACGTCAGCAGCTACCGGATTTATTCTCACGGCCAACGTCCCGGCGGCAATAGGCGGTGG TTCTTCAAAGAGCACCACCATTGTGTCTTTCTTGCCGATGAGAAGCTTCGGTTCAAGGCTAGTAGTCAGAGCGGCGGAAG ATACACCTCCGGCAACCGCCTCGTCGGATAGTTCTTCCACCACCGCTGCTGCTGCTCCGGCGAAAGTTCCGGCCGCTAAG GCTAAACCTCCTCCAATTGGCCCAAAGAGAGGATCCAAGGTCAAGATTCTAAGGAAAGAATCATACTGGTACAAGAACGT TGGATCAGTTGTGGCCGTTGATCAGGACCCGAAGACCCGATATCCGGTTGTGGTCCGGTTCGCCAAGGTGAATTACGCGA ATATATCGACCAACAACTACGCATTGGACGAGGTGGAAGAAGTAAAATGAGAGACATGGTTTATCTCTAAACCGGTTCGT CTGTTTGTAATCTTAAACAAAACCGTATGTACCAAAAAGTTAACCAACAATTCATCTTTTATCTTTTAC ................................N......N........................N............... .................N......N....................................................... ......................................................N......................... ................................................................................ ............i.....i............................................................. .....................................i.......................................... ................................................................................ ................................................................................ ................................................................................ ..............................................N........N........................ ..........................N.......................................... Pos Score Pred ------------------------ 33 0.352 - 40 0.489 - 65 0.251 - 98 0.146 - 105 0.387 - 215 0.421 - 333 0.689 Yes 339 0.737 Yes 438 0.669 Yes 767 0.289 - 776 0.345 - 827 0.036 -

Explain the output -- Processing locus 1081: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTGTTTAGATGATGAATTTTCAATTATCGCTTCTATGTATAGAATTGAATGTTCTGCATCTTGCTATATATGTAGTATA CTTGATTAGTTTCGTCGCTGACCTTGTGATATGTTTGCAGAATCAATTATGAAAGGAGCTAAATCAAAGACTGAAACCAG GAGCTCCAAGCTCTCTGTGACCAAGAAGCCGGCTAAAGGAGCAGGGCGTGGCAAAGCTGCTGCTAAGGACCCCAACAAAC CAAAGAGGCCAGCCAGTGCTTTCTTCGTTTTCATGGAAGATTTCCGTGAGACTTTCAAGAAGGAAAACCCCAAGAACAAG TCTGTAGCTACTGTTGGAAAAGCTGCTGGAGACAAGTGGAAGTCCTTGTCTGATTCTGAGAAAGCTCCTTATGTTGCTAA GGCTGAGAAACGCAAGGTTGAATATGAGAAGAACATTAAAGCTTACAACAAGAAACTGGAGGAAGGTCCAAAAGAAGATG AGGAATCTGACAAGTCAGTGTCAGAGGTCAATGACGAGGATGATGCTGAGGATGGTAGTGAAGAGGAGGAGGACGATGAC TAAGAAGCTGAATGTTGGTAGCATTAGTATAGATGGCTGCAAAAATCTCTCTGTTTTTATCTTTACTTGAAATGTTAACG GGGCTGATTAAAATGGGTCTTT .........N..N......................N.............N....................N......... ..............................i.................i............................... ................................................................................ ................................i............................................... ......................................................................N......... .......................N.....................................................N.. ..............................N........N..N........N.......................i.... ...........N....................N......................................N........ ............N......... Pos Score Pred ------------------------ 10 0.305 - 13 0.304 - 36 0.290 - 50 0.264 - 71 0.309 - 111 0.673 Yes 129 0.837 Yes 273 0.616 Yes 391 0.199 - 424 0.202 - 478 0.213 - 511 0.191 - 520 0.168 - 523 0.117 - 532 0.100 - 556 0.642 Yes 572 0.065 - 593 0.131 - 632 0.040 - 653 0.064 -

Explain the output -- Processing locus 1082: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTGATATGATAAAAGTATCATGAATTTCACATTAGGAGTAGTAATTAAGTATGGACAGCTAAGTTAGTAAAAAGAGAG ATGGTGTTGATTGGTCATTAATTTATACATTATTGTTCAATTGTAAAACCATCAACCTTTTTGATGCTTATAAAACGAGA CAACAAATCCTGCAGACTACACATTATAAGAAAACGAGAGAAAAAGATATACTATCGATCAATTGAGAAAAAGGGTCTGT ACGGTTTAGTAATGCTAATGTACTTGGTGGGTAGTGGCTTTAGTCAAGGGGAAGTGACATGGTTGAGTAACGAAGTTGAA TGGCGACTTATTCTGACCACGACTCAACTTCCTATCGTGGTTACGGTAACATCCTCGTTGTGCGATGACCGGTGCACTAT ATTGGATGATCAACTGGCCCGATTGTCTGAAACCTATGGTAACCTAATTATATTACGTAAAGCCAATATTTTGACGAATC CATTCTTTGCAATACTTTACAATTTGGTCAATGCCCCGATCATCATAGTCTTCCAAACTGGAAAAGAAATAGCACGTTTA GAAAATGATTTATCTTGGGGTGCAATCACTGACATAATCGACAATCTTTCACTCTTCGCCCCGCCCCCCTCGGATTTTGT GCAGCCGCCCTCCGATTCCGCCCCAGTCCCCTCTCTCGGAGAGTAAATGATAGTCTCATCTGAATCAGTAACTTCAAATA TTTGCCTATGTATTTTGTGTTTGCTGCTTCCGCTATCAAACATTTGCCTATATTATTGTATTATA .......N.............N..............................i........................... N..............................................................N................ ................................................................................ ...........N.....N........................................N....................i ................................................................N............... .....N.............................N............................................ ..............................N................................................. ....i........................................................................... ..............................................N................................. .......N......................................................... Pos Score Pred ------------------------ 8 0.408 - 22 0.216 - 53 0.520 Yes 81 0.411 - 144 0.420 - 252 0.400 - 258 0.258 - 299 0.470 - 320 0.612 Yes 385 0.167 - 406 0.152 - 436 0.164 - 511 0.343 - 565 0.546 Yes 687 0.127 - 728 0.113 -

Explain the output -- Processing locus 1083: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGTTTGATTTATTTTAAATTTAAACACTTCAACATGTTTGTAATAAAAAAAAATCAAAATAAGAAATTCTCGAATGTATT TTATATTCAATCAACGAATAAAATAAATTATTCCACGAACATGAACTGCATGCGTCCCACCTCATTTTATACGAAATTTC CATGTTACCCTCTCTAATACTATTCTCCTTCCTCTTCCACAGACCCAATTTCGTCATTCAATTCCAAAACTCACACCTCT ATATAAATCCAGCAAAAAGTCCCCAAAAAGTCACCACCACAAATCTCATCGAAATGTCGTCTCAAGAAACGAAATCAGAT CAAAGAGAAGGAGCAGAGATCTGCAATGGCGAATCAAACTGCAAACAGAAAGCCAAAGAGATTTTATCAACCATGAATCT ACCCAAAGGTCTTCTTCCTCTAGACAACATGACAGAGATCGGTCACAACAAATCAACCGGTTATGTCTGGATCAAGATCA AGAACAAGGTTCAACATCGGTTTAAAGCCATTGGACGAAACGTATCTTACGATTCGGAGGTTACTGCGATCCTTGAGAAT CGTCGGATGAGTCAGCTCACGGGAATCAAGAGCAAAGAGATTTTGATTTGGGTTACGATCTCTGAGATCTTTGTTAATCA TCAAGATCCGACTCAAATCACTTTTGCTAATCCTACGGGACTATCACGTACTTTCCCCGTCACTGCTTTTGAAGAAGACG AATGATTCATTTGTCAACGCTCACCATCATGATACTTTGCTTAGCTAAATTATCTTTTGTTTTTTCCTTATAATTCCTTT TGTTTAGCTAAGTTCTATTTTCAA .................................N.......................................N...... ........................................N........N.............................. .....................................................i.......................... .........................N..............................................i....... ............................i.................................N................. ................................................................................ ......N......................................................................... ................................................................................ ........................ Pos Score Pred ------------------------ 34 0.413 - 74 0.340 - 121 0.472 - 130 0.113 - 162 0.385 - 294 0.773 Yes 346 0.476 - 393 0.663 Yes 429 0.614 Yes 463 0.352 - 567 0.488 - 722 0.271 - 749 0.060 -

Explain the output -- Processing locus 1084: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAAATCTCTTGTAAATTATTCTATGCTACTTACTTACGCTTTAAAACGAATTGTAAACCTTGCTTCTCTCTGTAGTGA CAGTGTTTTTGTGGGTGTTGATTTTGTTGTTATTTATCTTATCAGGTTATAATGCTAGAATTGTATTCTGTTTGTGGCTT GTGGAAGTTGACAAACAATATGACTTGGGAATTTGAATTAGACATTGATTTGGGAGCTTCTTTAGTGCTCATTGATGAGC ACATTACCTATCACGCGTTGGTTGATATTCTTGTAGAGGATTTTCAAATAGATAGTTCAACCAATTCTTTGAAGCTTAGT TACAAGTTAGTCTCCACGTCTACAACCATACAATCTCATCCTCTTTATATTAGGAACGATAGACAGCTTGCAACTTTTAT GCACAAATTCTCACAGAGTGGAGGATTGCTTCAGTTATGTTTGACTGTAGAGCAAATTTCTGTGACAGTTGAGCAGGCTG AGACTACTTCAAATCCTTTTGTTTCTCATTCTGTCCCTAATGCTCAGGCTGAGACGACTTCAAATCCTTTTGCTTCTCAT TCTGCATCTACTACTCAGCACGAGGGGGGAATTGGCCCAAAAATTGGATAGGGGTCAAGACACAATCGAGCGTCGGCGGC TTACTCAAGTGCCCCACGAACCACATGATTTGTTTTCGATCAGAGCATCCAGCAGCGTAATCCGATTCTA ........................N....................................................... ...................................................i............................ ...................i......................................................N..... ................................................................................ ..............................................................................N. ....................................N........................................... .......................................N........................................ ................................................................................ ........................N............................................. Pos Score Pred ------------------------ 25 0.428 - 132 0.530 Yes 180 0.666 Yes 235 0.147 - 399 0.340 - 437 0.427 - 520 0.097 - 665 0.053 -

Explain the output -- Processing locus 1085: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAATAAAATGAAGAGAGATCAGAGAATTTCGCTTCAAATGGAGTTGATGGGAAAATCTTAGCTCTCACCTCTATGAGAG GATATTGGATAGGATAATTAGATTTTAGAGCTTTATTCCTCTACGAATGACTCATACCAGATTATGGTGTTCTACTACCT TAATCACCGTCTGTTTCGCAATCTCGTCGATTTATCTTTTATTGGGCCGGAAAGTAAGTATTATCAAGGGCTTAGCCCAA ACGCTTTATGCGGTTCACGCGTGGAATCGACGCGCCGTCACCGTCAACAAAATTGGGAAGAGAATATAAAATTAGGGCAA GAGAGAAAAAAGGTTGAATCGAAACAGATCGGAAAATCGTCGAGAGAGAGAGAGAGAGAGAGAATGTCGTATCTTCTTCC ACATCTGCACTCCGGTTGGGCTGTTGATCAGTCGATTCTGGCCGAGGAAGAGCGTCTCGTCGTCATTCGTTTCGGCCATG ACTGGGATGAGACCTGTATGCAGATGGATGAGGTGCTTGCGTCTGTTGCTGAGACGATTAAGAACTTTGCAGTCATTTAT CTGGTGGACATCACTGAGGTTCCAGACTTCAACACCATGTACGAGCTGTACGATCCTTCTACGGTCATGTTCTTCTTCAG GAACAAGCACATCATGATCGATCTTGGAACTGGTAACAACAACAAGATCAACTGGGCTCTCAAGGACAAGCAGGAGTTCA TTGATATCATTGAGACTGTCTACCGTGGTGCAAGGAAGGGTCGTGGGTTGGTGATTGCTCCAAAAGATTACTCCACCAAA TACCGTTACTAATCGAGCTTCCCAACACTATCTAGTTTGTTAAAACCATTGAGTCTAGTGATTCTGGTCAGCTGAAATAT CCCGTGAACTGGTTTCATTTCATATATGCTT ........i.............................i........N.........................N...... ..............................................N................i................ ................................................................................ .......N........................................................................ ...............................................................i................ .............................................................................N.. ......N..........i.....i...N.................................................... ....................................i.............................N............. .............i.................................................................. ................................................................................ ................................................................................ .........................N..... Pos Score Pred ------------------------ 9 0.657 Yes 39 0.610 Yes 48 0.261 - 74 0.137 - 127 0.375 - 144 0.718 Yes 248 0.440 - 384 0.720 Yes 478 0.093 - 487 0.115 - 498 0.860 Yes 504 0.766 Yes 508 0.165 - 597 0.711 Yes 627 0.495 - 654 0.582 Yes 906 0.032 -

Explain the output -- Processing locus 1086: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATCTTATTCTTTGTTTTAAACCCCTCCATGCACTCCTCTTGTTTATATATTTATTGCTTCTTCTCTAAGTTTGTTGAGT CCTAATCATCTCTCTATCTAGCAACGTATAAATATAAAGAGAGAGAGCAGAGAAATGGGTTTCGGCTCCTTCATATCTAC CATCACTTACTGTTTGTTCTTTCGCTATCCACATAAGAAGCCAAAGTTCAAAAGTGTGAGCCACCTCAACCATTACCACA CCATGCCAATGGCTCGTCCACTTTCTTTACAGACGATAGACCTGAAAGTAAGGATGTGTTGCAGTGGATGTGAGAGGGTT GTCAAGCATGCCATTTACAAGCTGAGAGGGGTGGACTCGGTGGAGGTGAACCTAGAGATGGAGAGAGTAACGGTGGTTGG GTACGTAGAGAGGAAGAAGGTGCTCAAGGCGGTGAGAAGAGCCGGTAAACGAGCCGAGTTCTGGCCCTACCCGGACATGC CTCGCTACTTCACTTCCTCTGACCATTACTTCAAAGATACCACCCGCGAGTTTAGGGAGAGCTACAACTACTATCGCCAT GGCTACAACCTTAGTGACCGTCACGGTAACATTCACGTCACCAACCGTGGTGACGACAAAATGAGCAACTTTTTCAACGA CGACAACGTCCACGCATGTAGCCTTATGTAATTTCCTCACTTCTTTTTAGGTATCTATGTGTTTATATTTGTTGTATCAT TTATAGGGTTGATTGAATAGCTTTTATGCATAAGCGTAAGTGAGACAAGT ............................N................................................... ......................................................i......................... ................................................................................ ..i.....i............................................i.............i............ .......N.................................................i...................... ............................................................................N... ..............................................................................i. ............................................................i................... ...............N.........i..............................N....................... .........................N........................ Pos Score Pred ------------------------ 29 0.388 - 135 0.793 Yes 243 0.661 Yes 249 0.766 Yes 294 0.761 Yes 308 0.625 Yes 328 0.491 - 378 0.656 Yes 477 0.267 - 559 0.697 Yes 621 0.519 Yes 656 0.361 - 666 0.577 Yes 697 0.063 - 746 0.055 -

Explain the output -- Processing locus 1087: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTAATAGGAAATGATGCTTTTGACTTTTTGTTCTTCAACGGTTACAAAATAGTAGTATCCTTTTGATACAAAACGCAGT GACCATTTTCTTAAGCATTAACATTACTCAATGAAGATTATGAAAGCAATGAAGAAGCTCACGTTCTGGTCAAGAAAGAA GAGGAAACGTAAGCAAGCTTGCCCATCTCAGCCTCACCATTGCTCTTGTGAGTACTCTTCCACGGCAGTAGCGGTGTTGG TTGAGCCCACAGCGCCTCCTCTTCCGTATTGGTTCGATGAGACTCGGAGTCTTTGTCCCCCAGAGACATCATCATTTCCT TGGACTACTCATCACTTACCTCACCAACAAGAAGAAACCATCGTTGAAGCAACCCCGTTGTTGTCTCAAGTGTCTGATCT TCGCATCTACCAGTCTTATCAACAGTACATGGTGCCGAATCCAACTAGTAACGTCCACTTTGTAGAACCAGCTACAGCAA AGAGATCAGTGGGAATATTCGGTTGTGTCATCGAGTTAAGTTCGAGTCTAGTTCGTTGTTTCATACCGTAATTTGAGCAG TTTTATGGAAACCAATTTGCGAGAATAATTCAACATCTTTTAACATGTTACGTGTATCATCTTTGCATCTTCTCAATTAC ATAAGCATCG ...........N..i................................................................. ..............................i........N........N............................... ................................................................................ ....................................N........................................... ................................................................................ ............................N................................................... ................................................................................ ....N.......................................N................................... .......... Pos Score Pred ------------------------ 12 0.481 - 15 0.642 Yes 111 0.560 Yes 120 0.406 - 129 0.457 - 277 0.305 - 429 0.452 - 565 0.053 - 605 0.074 -

Explain the output -- Processing locus 1088: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTGTGTAAATTTCCTTTTTTTAGGGTACAAAAAAATGCAGAACTTTCCTTTTCTCGGTTAAAAAAAAAAGGAAAGTAA ACAACGAACACCATTACTTAACTTTGGAGTGAGTTTCTAATACTGAGTTACTTGAGCCGTAACCGTAAGAGATCATTCAT GCTTGAGAGAATGTCAAAAAAGATTAACGAGCTTCTTCGTTACTGTGAAGAGAACTATATTGAGAGAGGAAGTCTCGAAC TTGCATTACGATGTGCTGTTTCAATCTGCAAAGCGAATCCTGACGCTCCTCAAGCTTATGCACACGTCGCCGCTTACAGA ATCCTTTTGACGGCAGCCAATTATCGTACGGTCACAGGAGAGCCTGACTGGTACGCTGTGTTGGGAATCAACAAACGCGG ATCCTCAAAGTCTGTGGTGAACGCTATTGATAGGCGGTGCGAGGAGATCATAGAGGTGTTGGATGGCGAAACTGGGGTCT CCAAAGCTGTTTCGAGGGTTTATGATCTGGTCAGACTTGGCGTATCGGAGTTGATGGACGAAGATAGGAGAAGAGCGTAT GATCTCCGTTCTGGATTTTCTATTATCAACTGAATCTAAAACTTTTTGAAGTTTTAATTTTTCAAGCAAAGTTTGTAATC TCTCTATGTTTTCCTATGGATTCATGTATGTATATGTCTTTCCATGTTGAAC ....................................i........................................... ..............................................................................N. ..........N..................................................................... ..........N..............................................N...................... ................................................................................ ..............................................................N................. .....................N...............................N........................N. ................................................................................ .....N.........N.......N...N.....N.........N........ Pos Score Pred ------------------------ 37 0.550 Yes 159 0.178 - 171 0.281 - 251 0.241 - 298 0.182 - 463 0.320 - 502 0.083 - 534 0.159 - 559 0.213 - 646 0.088 - 656 0.092 - 664 0.057 - 668 0.036 - 674 0.065 - 684 0.039 -

Explain the output -- Processing locus 1089: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TACGTTGTTACCACCAAACTTTGGAGGCTCGCTAATAATAACCACACTACCCATTTGTTACACACCCTTTATTTTCAACC ATATCATCTCACCTTCGTTAAATGTTCCCACAATTAGCTCAGTATTTTACTATATACATACACACACATTCCCTCCACAG GATCAAACAAACACACGAGCTTTCTCCTCTACAACAAAATAAAATAAAATTAATGGCTTCTTCACTTATCACCTCCGCAG TCATTGTCGTGGTTTTAAGCCTAGTGCTTGGATCTGTAGAGCAAGTGAGTGGACTACGTCACGTTCCCAAGTCCCCTAAG ACCACTGATGTCAAACACCCTGACTTTCTTGTAACCATTGAGCCCAAACCAACTATTCTCATTCCCGGTGTTGGAAGGTT CTTGCTTCCTCCCAAATGCAAGAAGCCGTTCTACCCTTACAATCCTGTCACCGGAGCTCCACTTACTGGTGGGGGAATCC CATCATATAATGGTGGACAAGGGGCCGGACCTCACACCCAACTCCCTGGTGGCGATGATACGCTTGTCCCAAACCCCGGA TTTGAAGCGCCAACCCCGACCATTGGAGCTGGCACCGGAAGCAACGGCCAAGTTCCACCAGTGCCACTACCCTGAGTATT ATTAATCTGTCAACAAATAAGCATATCTTAGATGCAAACATGTCTGTTTTGGTGTCTTGAGTCTTGGTTAGATAAGTAAC CCGCTACTTTACTA ................................................................................ .....................N.......................................................... ....................................................i........................... ................................................................................ .......i........................................................................ ...............N................................................................ .........N............................................N......................... ................................................................................ ...............................N.......N........................................ .............. Pos Score Pred ------------------------ 102 0.389 - 213 0.851 Yes 328 0.553 Yes 416 0.441 - 490 0.471 - 535 0.335 - 672 0.056 - 680 0.123 -

Explain the output -- Processing locus 1090: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGAAGCTCCCTATACATAAAACCATTACATTTCCCATAGAAGAGGACTGTCATGACCAATGATAATTGTGAACAAGTCAT TAACACTTTCTTCACCTTTAATTAAGTCTTATCCAATTAATTATCTTCAACTGTAATCCGTCTCTTTCCTTCTATATAAT CCACTACATTCCATGCATGCATGCTTTCAAATCCAAACATAACATCTCAACATTGAACTCGTTTGAAATCTCTAAACAAG CGATAGAGCTAAACAAAATGGCAATGAGGTTCTCATCACTTACTAAAATCGTGCTTCTCTCTGCAGCTATGTTGCTTTTG GTCCACGCAAGTGCTCGCACTTTACAGCAAACTCAGACTCTGCCTCTCCAAGGAGGAGGAGCTGCTCCTACCAAAGATGA GCCTCATATGGTTAATGCCCCTCTTGAGGACCAGAAGAACTTCATCTACGGCGGTGGAATTGGCGGTGTTGCCGGAGTTG GTGGATTCATGGGGATGCCAGGTGGTGGAAGTGGTGGCTCTGGCATGACTTTCCCACTCCCGAGTGGGACGCCGCTTCTT GGTGGGGCTGGTGGGCTTGGTGGTTTGGGTGGTGCAATGGGGTTCCCTGGTGGACTCGGTGGTGGACCTAGTGGTGGTGG TGTTCCAAGTTCAAGCGGTGGTTCTCCTTAAGACAGATCATGATGATTAATTGGTTCCTATGTAGTATGTAACTCCAAGA TGTTTAGGAGGAAGTGAAGTTTTGTTTCGTTGTGTTAAGTTTGGATCTTT ...................................................N......N..................... ................................................................................ ............i...i...N........................................................... .................i.....i............................................i........... ............................................................................N... .......i......N................................................................. ........i.....i.............................i................................... ....................................i........................................... .......................................N..N................N......N............N .................................................. Pos Score Pred ------------------------ 52 0.377 - 59 0.235 - 173 0.607 Yes 177 0.579 Yes 181 0.154 - 258 0.748 Yes 264 0.714 Yes 309 0.570 Yes 397 0.464 - 408 0.835 Yes 415 0.300 - 489 0.789 Yes 495 0.756 Yes 525 0.540 Yes 597 0.545 Yes 680 0.075 - 683 0.072 - 700 0.077 - 707 0.043 - 720 0.115 -

Explain the output -- Processing locus 1091: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTTGTAACCATATAGATTCAGCTATTAATCACCTATAACGTAGATATTGTTTATGGCCCAAATTTGAGAAACTGAAGCT AGCTCATGTATATATATATGAAAAGCCAAGGGAGATACATTTGCACTCAAAAGATATACCATCCAACACCGAATCAAAAT TATGGACACACTTATATTGGTATCGATCATGGTCCTAATGCTCTTTGCTAGGTCTGGATCTGCACGTGCCGCCACACTTA TCGTATCCAACGAGATGCAAACACTTCCAAATGTTCCCGTTTTCGTCACGTGCCGCCCAACCCCTGAACTGTCCAAATCC GTACCTTTGGGTCAAAAGATTATGATAGAGATCCCAAGCATTGCCGGCGATAATGAAAAAGTGGCGGGATCTAGACAGTT ATCACATACGCTATGTGTAGGAACGTTTTACAATAAGAAAAGTTCCGCAGCTTACGGGATGTGGTTCGTTTTGTACGATT CATCAGACAAAGATCACTGCAAAGACTCGTGTTCCATTTTTGCCAAAGACTATGGATTTTATAGTTGGAACAATAACAAG AAAACCTTCGATCTCATCCCTCCAAGGTTTTGGATTAACTAGTTAATCTTTTATTTCCGTTTAACGTTATACTTCCAGAT TCGAACTATATAAATTTGTGATATTTGACTACAATTCAAACCCAATTTATAACATTTGATA .....................................................N.......................... .....N...........N.............................................................. ..............i...............N................................................. .....................N..............................N........................... ............N.............................................i..................... ...................................................N............................ ................................................................................ ............................................................. Pos Score Pred ------------------------ 54 0.369 - 86 0.272 - 98 0.309 - 162 0.853 Yes 189 0.517 Yes 198 0.377 - 255 0.588 Yes 271 0.259 - 342 0.364 - 373 0.312 - 413 0.178 - 459 0.521 Yes 532 0.334 -

Explain the output -- Processing locus 1092: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGTTAAGATTATATGGAATGTCCTTCATTGTTCTGCTTTCTAATTCTTGTGTCAATCTTATCTAAATCTTATAAATCAG CATTTGCATTAGAAGATTTGATCTATTTTAAATTGGTTAAGAAGTGAAATGATTGATTGTCAATGCAGGCTCTTGTTATT GAAAATGGCAGATTCCACCAAAGCAGGGTTGAAGAAGCCAGCTTTCACTAAGGTTGATCAGCTGCGTCCTGGGACTAGCG GACACAATGTCAATGTGAAGATTGTCAGCACGAAAATGGTGTTACAGAAAGGTCGTGCTGATGGTCCTCAAGCTCGCCAG TTGCGGATTTCTGAATGCATTGTTGGTGACGAGACAGGAGTGATCGTCTTTACCGCAAGAAATGACCAAGTGGACTTAAT GAAAGAGGGATCAACTGTAACTCTACGCAATGCGAAAATCGACATGTATAAAGGATCAATGAGGCTAGCTGTAGACAAGT GGGGTCGTGTTGAAGTCACAGAGCCTGCGAGCTTCAAAGTGAAAGAAGACACCAACATGTCCCTTATCGAGTATGAGCTC GTAAACGTCGTTGAATGAGTGACAAATTCCTCGAAATACCACTAGGAGAAACGCCCTCAAGAATAAACAAGTCAAATCCG TAACCGTTTATGTTAGCGTCAAGAAAAGAGTTAAACC .............N....N............................................................. ................................................i.............i................. ....i........................................................................... ......N.....N......................i........................N................... ..............i..............................................N................i. .............................N.............i..............i..................... ........................................................N...............N....... ..............N................................................................. .........N........................... Pos Score Pred ------------------------ 14 0.363 - 19 0.478 - 129 0.792 Yes 143 0.555 Yes 165 0.779 Yes 247 0.232 - 253 0.150 - 276 0.746 Yes 301 0.159 - 335 0.525 Yes 382 0.224 - 399 0.707 Yes 430 0.469 - 444 0.544 Yes 459 0.685 Yes 537 0.372 - 553 0.196 - 575 0.468 - 650 0.059 -

Explain the output -- Processing locus 1093: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAAATGCTAAAATTAGTGTGAAAAACGTCCGGAAAGAAAGGATATGGTTAGGCGAATTTGAAGGATAGAGAGTAAAAAA AGGTGTGGTTTGATGTTATAAGATGGAGAGGTATTAATGGTTGTCCGAGTTCGTCTCCATTGGCCTCTTCAAATCAAATT AAGAATCTCTTATTCCCCCAAATTTTTCATTTCTCTTTTTCTTCATTCTTCATCTCTCGTCCCACAAAAGAGGACAAAAA ACCAAATAACGAGAAGTCCCATTTCTCTTTGTTGTCTTAGGTGTTACCTTCATCAACCAAACGCAATGGCGAACGCGGCG TCGGGGATGGCGGTGGAGGACGAGTGTAAGCTGAAGTTTTTGGAGCTAAAAGCGAAGAGAAACTATAGGTTCATAATATT CAGGATAGATGGACAACAAGTGGTGGTAGAAAAGCTGGGAAGCCCCCAAGAGAACTACGACGATTTCACCAATTACCTAC CGCCAAATGAATGCCGCTACGCCGTTTATGACTTCGACTTCACCACTGCTGAGAATATCCAGAAGAGCAAGATCTTCTTC ATAGCATGGTCACCGGATTCATCTAGAGTAAGGATGAAGATGGTGTATGCGAGCTCAAAGGACAGGTTCAAGAGGGAATT GGATGGTATTCAGGTGGAGTTACAAGCCACTGACCCGAGCGAGATGAGTCTCGACATCATCAAAAGTCGAGCTCTCTAGA TCTCCTTGCTTTTCTAATTATATATCCATAATCATTGTCATTCAAACAATTTCCTCATCTTATGTAACTTTCGATCTTGG TTTCATTCATTCTTGTTC ....N.......................................N................................... ............N.........N.............N........................................... ................................................................................ .................................................................i.............. ......N......................................................................... ........N....................................................................... ......N...i................N.................................................... .....N...........................i.....i......N................................. ..N........................................N.................................... .............................................................N.................. .................. Pos Score Pred ------------------------ 5 0.323 - 45 0.343 - 93 0.077 - 103 0.174 - 117 0.493 - 306 0.631 Yes 327 0.306 - 409 0.353 - 487 0.256 - 491 0.602 Yes 508 0.216 - 566 0.402 - 594 0.701 Yes 600 0.859 Yes 607 0.299 - 643 0.174 - 684 0.105 - 782 0.047 -

Explain the output -- Processing locus 1094: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCACACTTCCAATCCTCGACCTTATCCACCTTACGTAGACCTAATATCCAGTCGCCACGTCACACACAATCAACGTCTCA GATGTACCCGATCGCCTTTAAATCATCGCTTCCTTCATGAATCGAAGAACCTCTACAACAACAGATTTTAAAAAACCCCA AAAACTTTACATTCTTAACAATTTCAACCCTATGGGCTTACTTGATCATCTCTGGGATGATACTGTCGCTGGTCCACGGC CAGAGAACGGCCTTGGCAAGCTTCGTAAACACCATACCTTCAGTTTCCGACCTAGCTCCGGCAATGATCAATCGGAAGCT GGAAGTGCGAGATCGTACGGTGAGGATTCGCTACCGGAAGAAGCAGTGAAAGTTACACGTAGTATCATGATAATTAAACC ACCGGGTTACCAAGGCAGTTCAGCTCCAGCTTCGCCTGCCGGTTCAACTCCGCCGCTATCACCTTTCTCTCCGCCGCTGT CTCCGTTCTCTGGGGGAAAGGAACCCTTTCGGTTTAGGAGACGGTCGACGTCGGATGCGTTCGAGAAGGCAGCAGGAGGA TCAGAGACTGGACCAAGGAGCTCTCCTCCTACTTACGGCATGTGATCTCTAACACACAACACAAGTCTTGAAAAAGAGAA GAGAAGAAGAAAACAGAGTACCCACCCTTAAACGATGTCGTTTAGACTGCATGAAGTGGTGGTT ................................................................................ ...............................i........................N....................... ...............................................................N................ ..................................................................N............. ................................................................................ ......................................................i......................... .......................................N........................................ ..................................N...............N............. Pos Score Pred ------------------------ 82 0.385 - 117 0.347 - 192 0.500 - 217 0.435 - 304 0.198 - 387 0.260 - 535 0.616 Yes 600 0.242 - 675 0.070 - 691 0.037 -

Explain the output -- Processing locus 1095: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGGTCAGAACTTAGAACTTGACTAACTCTGCTTCGGTTAGGATCTGATTGGTACGGTTTGGTTCGATTTTCGTCAATAG AATAAAAAACTGTATGAATAGAATAATCCGGTTAAATTGGCACGTGAAATGCGAAATGGGCTGAGTCATTCATTATGGAA AGCCCATGTGATTGGTAGGGAACGACGTCGGGTGAAGGGAGGGGAAGAGCTTATAAATGAAAGCAGCAAGCAGAGCAGTT CACGCTCTTTGTACACTCCGCCCTCTCTCCCCATCTCTTTCGACAGATCTCTTCTCTCTCTCGTGTTTCACGAAACAATG GCGACTCAATTCAGCGCTTCTGTCTCATTGCAAACTTCTTGTCTGGCAACAACAAGGATTAGTTTCCAAAAGCCAGCTTT GATTTCCAACCATGGAAAGACTAATCTATCCTTCAACCTCCGCCGTTCAATCCCATCTCGCCGCCTCTCTGTTTCTTGCG CGGCAAAACAAGAGACGATAGAGAAAGTGTCTGCTATAGTTAAGAAGCAACTATCACTTACACCGGATAAAAAAGTCGTT GCAGAAACCAAATTTGCTGACCTTGGAGCAGATTCTCTCGACACGGTTGAGATAGTAATGGGTTTAGAGGAAGAGTTTAA CATCCAAATGGCCGAAGAGAAAGCACAGAAGATTGCCACAGTTGAGCAAGCTGCTGAACTCATTGAAGAGCTCATCAACG AGAAGAAGTAATTTTAGTTTTCTTTTATAAAATGCCCTTAAATAAACTAAAAAAGAAAACGACAAAAAAAAACAGGGCGT TGAGTTTGTTTTCATTATGTTTGATTTCTC ................................................................................ .............N..................................i......i..................N..... .....N..................................................N....................... .............................................................................i.. ................................................................................ ...........i.................................................................... ................................................................................ .........................................................i...................... .......N........................................................................ ...............................N................................................ ................N............. Pos Score Pred ------------------------ 94 0.327 - 129 0.784 Yes 136 0.606 Yes 155 0.354 - 166 0.220 - 217 0.339 - 318 0.850 Yes 412 0.637 Yes 618 0.811 Yes 648 0.432 - 752 0.089 - 817 0.058 -

Explain the output -- Processing locus 1096: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAATACGTAAGTAACTTTTGTGAGTTTTTGTCTGCGTGAGAATTTTAACGTTTGTTGTAATCTAGTTGAAATTGAAATC GGCAGGTAACAAAAAGTCTGAATTTATGATTCTTTTATGACACAACACACAAGTCATGAAACTTATGAGGCAATTCTTAT ATTTATGTGTGTAGAGGCGATAATGGTGAGGGCTAGCGTCTTAAACGATTGTCTCAAGAGCATGAGTAATGCAGAGAAAC AAGGGAAACGCCAAGTCATGATAAGACCATCTTCCAAAGTGATCATTAAGTTTCTCACTGTGATGCAAAAGCATGGTTAC ATTGCAGAGTTTGAATATGTGGATGATCACAGATCCGGGAAGATTGTGGTTGAGCTTAATGGAAGGCTTAACAACTGCGG TGTTATTAGTCCTCGATATGACCTTGGCGTCAAGGAAATTGAAGGTTGGACTGCTAAATTGCTCCCTTCAAGACAGTTTG GGTACATTGTCCTGACAACATCAGCTGGAATCATGGACCATGAAGAGGCTAGGAAGAAAAATGTTGGGGGCAAAGTGCTT GGTTTTTTCTACTAGAGGGATTTTTATGATTTTATGTGTTGGGACTTAATTGTTAGTCTTTTTAGTGTTTTCTTATTGAT TTTTCGATTGGTTTACTTATTATTACTCTTTTGG ................................................................................ .........................N..........i..................N........N............... ....N.................i......................................N......N........... .................N............................................N.........N....... ................i.....N...................................N..................... .................N.............................................................. ................................i......N....................N................... .........................N.......N.............................................. .................................. Pos Score Pred ------------------------ 106 0.144 - 117 0.604 Yes 136 0.186 - 145 0.179 - 165 0.477 - 183 0.620 Yes 222 0.254 - 229 0.205 - 258 0.296 - 303 0.317 - 313 0.332 - 337 0.718 Yes 343 0.186 - 379 0.139 - 418 0.266 - 513 0.580 Yes 520 0.398 - 541 0.331 - 586 0.040 - 594 0.053 -

Explain the output -- Processing locus 1097: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAGTATCATAATTTACAAGACAACAAGTGTATCGTAGCCACAAGACACGTTCAATCTCAATGCCCTTGTTAGTCGTCATT ATTCAATATATTCATCACTAGTCGTTTGTCTTAACTCACATGAAACAACTTATTTTAAAAGCCACTCACTTACTGATTTC CACTCAGACTCAAGAAGAAAGAAAGTCTCGAAAATCTTCTCTCTTTCTCTTACACAAACATATATTTTCTTGTTTACCAT TAAACAAAATCTTTCATAAAAAATTGGCCATGAATAAACGTTACGGTCACCTCATAAGTCTATTGTTCGTGTTTTCTCTT GTTATGTTCCCCTTCATAACAATATCACAGACGCCATGTCCGTACCCTTGTAACCCGTTGCCAATAGCCGGTACTGGCTC CACTCAACCGGCCGGTTATTATCCTCAACCGACCGGTTACTATCCTCCTCCATCGTCGAGTAATGTACCGAATTACCCTT CTCCACCTTATTACGGAGGTAATCCCAGCGGGGGATATAACGGTCCTCCACCGCCGGACCCTATCTTGCCTTATTTCCCG TTCTACTACAGAAAACCTCCTCATCAGACGGATCAGTCATCATCTTCCATGAAATCAACTGTCAAGATCGTAACAGTGGC AAATCTTCTTGCGGTACTTATGTTATTTTCTTGGTAACGTTTTGTTTATGGGATAATTGTAACTTTCTATGGTTGAAACT TTTATGTTTGTTAATCTTTCTATAAAAATTGCGAGGAGGAAAAAAATAGTTGAGTA ...........................................................N.................... .......................................N........................................ ................................................................................ .............................N.................................................. ...N...............................N............................................ ..............................................................N................. ................................................................................ ................................................N............................... ...................N...........................N....................N........... ...N.................................................... Pos Score Pred ------------------------ 60 0.356 - 120 0.158 - 270 0.243 - 324 0.359 - 356 0.386 - 463 0.447 - 609 0.330 - 660 0.302 - 688 0.115 - 709 0.117 - 724 0.036 -

Explain the output -- Processing locus 1098: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTAAAATCTCTTGTAAATTATTCTATGCTACTTACTTACGCTTTAAAACGAATTGTAAACCTTGCTTCTCTCTGTAGTGA CAGTGTTTTTGTGGGTGTTGATTTTGTTGTTATTTATCTTATCAGGTTATAATGCTAGAATTGTATTCTGTTTGTGGCTT GTGGAAGTTGACAAACAATATGACTTGGGAATTTGAATTAGACATTGATTTGGGAGCTTCTTTAGTGCTCATTGATGAGC ACATTACCTATCACGCGTTGGTTGATATTCTTGTAGAGGATTTTCAAATAGATAGTTCAACCAATTCTTTGAAGCTTAGT TACAAGTTAGTCTCCACGTCTACAACCATACAATCTCATCCTCTTTATATTAGGAACGATAGACAGCTTGCAACTTTTAT GCACAAATTCTCACAGAGTGGAGGATTGCTTCAGTTATGTTTGACTGTAGAGCAAATTTCTGTGACAGTTGAGCAGGCTG AGACTACTTCAAATCCTTTTGTTTCTCATTCTGTCCCTAATGCTCAGGCTGAGACGACTTCAAATCCTTTTGCTTCTCAT TCTGCATCTACTACTCAGATAGATTGATGTTTGAGGTAATGTTCTTTTATGTGTACTAATGAGAAACAATTAACATAGTT TTGTTAAAGAAATTGTTTAAAATCTAAACTAAATGATAATTTGTAA ........................N....................................................... ...................................................i............................ ...................i......................................................N..... ................................................................................ ..............................................................................N. ....................................N........................................... .......................................N........................................ ..........................N...........N.........N.........N..................... ................................N............. Pos Score Pred ------------------------ 25 0.428 - 132 0.530 Yes 180 0.666 Yes 235 0.147 - 399 0.340 - 437 0.427 - 520 0.101 - 587 0.354 - 599 0.068 - 609 0.092 - 619 0.081 - 673 0.040 -

Explain the output -- Processing locus 1099: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATGGCATTAATTAGAGATAAAGACATATAATCATGCCTGTTATAAATTTCAATAGTTGGTTCAACACATGCATAGGCTTT AAATACGTCTCCAGGCAATGATATAAATACATGCAAATGTACATGATAGACAACAAATCATCATCATCAAATATAATCAT CACCAGTTTCTCCATTTATTTTTCTACCAAAAAGCAAGAAATTTCTCAGAAAATGAAGACGAAGATGTTAATAACCCTCT TTGCACTAGTTTCAGTAGCAGGAACATCAGAAGCGACTGGTACACTTGCTGCACAGCAGAACACAAGCATTATACCTACA TACACACTCTGGTGCATGGAAAATCCATATGCCTACTTTAGAAGAGTGATTTCGAGCCTAAAATGGGCTTGCAAAAATGG TGCTGATTGCTCACCATTGGAAAAGGGTGGCCGTTGTCAAGACCTCGACAATTACAGAAGCCAAGCTTCTTATGCATTCA ATGACTATTACCAGAAGAATCCTATACCCAGAAACTGCGATTTCAATGGTGCTGCTGTGCTTACAGTTCAGGACCCAAGT AATACTAAACACTTTACATTCGAAAAAATAAAACCAACCTCTGATCAAAATTCCTAGAGTTCCATTGTGATAGTGTCTAT ATACGTCAGAACTGATTTTTCATAATCATCTTCAATTTGTGATTGTTGTTCTTCTTCTTATATCTGTGAACTTTGA N...............................i..................................N............ .................N............N.....i.....N..................................... ....................................................i...........i............... ................................................................................ ...............N............i.................................N.............i... .......................................................................N........ N............................................N.................................. ................................................................................ ............................................................................ Pos Score Pred ------------------------ 1 0.374 - 33 0.738 Yes 68 0.286 - 98 0.162 - 111 0.230 - 117 0.666 Yes 123 0.363 - 213 0.722 Yes 225 0.569 Yes 336 0.255 - 349 0.764 Yes 383 0.178 - 397 0.657 Yes 472 0.160 - 481 0.322 - 526 0.214 -

Explain the output -- Processing locus 1100: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAATTGCGGTAACGCATTAAATTCACGTACGCACACACCTAACTACTTCAATAAATCGACCCACCAACCAGACACATTG AAACAATGACAACTCATATAATTGATGATATACATTTCTCAAAGAAATCTCTTGAGAAATATTCACAGTCACTATTCTAA TCCATTTTCCATGGATTCCTCTCCCAAAATCACTAATCTCCCTTACAAGAGGCTTTCTCTAGAAGATGATGTCTTCGGCG AAACAACAAGAAGAAGATCGTCATTCTATTCTTACAGGTTCAAGCGAGTGGTGTCATTGGGAGGAAGGAGGAGGATTAGG GTTAGAGTTAAGATCCGAAGACTTAGAGGATTTGTGAGGAAGAAAGCGAATAGGGTAAAAACTGGAATTTTAAAAATATT GAAGAGGTTGAAGGAGAGTCAATCACACTTTGGTGATCTCTTTGCCGGTAATTACCTTTTCATGCAAGTTAATCCTTCTT CTCTTAACGCTAAGTATGTCTTCGACAAATCTTTTCAAGGCCAAAATGGTAACTTCCCCTCTAGATTATCTCTTCCTAGA GTTCTTATGTAAAGACGGATAAACATTAACTATAGTTATGTTTCTGTATTTTTTCTAATTACATGTGGTTTATGTGTCTG GTTTGTAACTTTTTCACGAAAAAAATAAAGA ................................................................................ .....N..................N....................................................... ..........i......................................................N..N........... ................................................................................ ................................................................................ .............................................................N.................. ...............i.............................N.................................. ......N..............................N........................N........N........ ............................... Pos Score Pred ------------------------ 86 0.351 - 105 0.192 - 171 0.614 Yes 226 0.244 - 229 0.312 - 462 0.196 - 496 0.582 Yes 526 0.385 - 567 0.251 - 598 0.082 - 623 0.078 - 632 0.069 -

Explain the output -- Processing locus 1101: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTATGGGCTTTGTAACCCAATCTGTACAAATTAGACTACTCGGTTCTGTTCGGTTTACTTTAATATTCTCCGGTTTAGT TGAACCGTTTCCTGAAAATGAATCGTCGTCTTCTTTGGGGCAAGCTCCTTGATGTTGTTGAGTCTAATCAGAGGAGGAAG AATGGCGGGAGGATCTGAGAGGAAGACGATACTGGTGGGTTTGGTTCTAGCTCTTGTTCTTGGTATCGGTGTTTACTTAA GGCTTTGGACTATCGATTATACTCTATCTTCCGATGACACAGAGCGCTTAAGGAGACAGTTTGATTTGGCAAATAGAGAG GCAATGGATGAATCTGCAGATTGGCGGAGAATGTTTGATAAGGAAGCTGAGAAAGCTTCTAAATGTAACACAGAACTTTC ACTGATCAAAGAGTCATCTGGGAATGGAAACACCTTTACCTCCAATCAAAAGCTAGAGTCATTAAAAAAGGAAAATGCGG CATTGCTCATTCAAATAGAGACATTAAAACAAGAGCTTGAAGCTTCACGGTTAAAGTGCCACTCACGGCAGGCACCCCGT TAGAATACTGTTCTTGATATGTTGACATGTTGTCAGTTATAGATTTAGTCCTCCGCTGGTAAGAAATCCCGGAATGTCAT ATACATCATATAGGCTCTTTCA ...N............................................................................ .................N.................................N............................ .................................N.............................................. ...i...N......................N...............................i................. .......................N..................................................N..... ................................................................................ ..................N.......N..............................................N...... ...................... Pos Score Pred ------------------------ 4 0.372 - 98 0.497 - 132 0.292 - 162 0.651 Yes 274 0.225 - 324 0.643 Yes 328 0.380 - 351 0.468 - 383 0.532 Yes 424 0.394 - 475 0.465 - 579 0.070 - 587 0.119 - 634 0.041 -

Explain the output -- Processing locus 1102: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTCCACAGATTTGTCATTTGTGTATCCGCCACTTAGTTGCTGTTGAGCTGTCCATAGCCTGTGTGGGTACTGTACTGGT CCCTACTAATACCAGCAATAATCGAAATTAATGGGCCTTCCTACGATCCATTATCAGCCCACTAGTTACTGTAGCGGACG GACTTTGTGTGAATTAACCGCCACGGAAGTTGGATTGAGGAATTCAGGAAAGAGCAAAAAAAAAATGGCGACTTGGATAA CGTTACAGCCTCCAATTTCATGTTATTTACGGCTGATGCCATCATCAGCGCCGTCGCCGGCTTCATCCACGTCGCGTCGA AAACAACGTCGTCTCTTTAAGCCATTTCGATGCGTTCCACTTCAAGAACAAGAGCCTCAGCTCGATCAGTCGCAGCAGGG AGACGACAATGCTGTGATTTTGTGTGAGGATTGTAACGGGAAAGGATGGTTGCTCTGCGATTTCTGTAAAGGACAGAAAA CAAATGTGAAATCTGAGAATAATCGAATCTATCGACGATGTCCAACTTGTAAAGCTGTGGGATTTGTGTTGTGTAGAAAA TGTAAAGTGTTCAAGTGTGTCACATTTCCTAACTCAGAAGACGGCGATGAACTTTTGTTCTAAGAGCTCCTTGTTCTAGC AACACTCTTCTTCTCCAACTAAGGACACCATTTGAGTTTTCAGGTTCCTTTCTTTTTCCAATTGTTATATATATATATTG TC ................................................................................ ..............................i................................................. ................................................................i............... ...................N...............N............................................ .............................N.................................................. ........N....................................i.................................. ...i.................................N.........................................N ..............................................N................................. ................................................................................ .. Pos Score Pred ------------------------ 111 0.659 Yes 225 0.656 Yes 260 0.400 - 276 0.313 - 350 0.324 - 409 0.236 - 446 0.542 Yes 484 0.505 Yes 518 0.271 - 560 0.300 - 607 0.212 -

Explain the output -- Processing locus 1103: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTCCAACTTTTTATCTAAGTATCGACTCTTGCAAGGGAAGAAGGTTAGCATATGTTGTAATGGTAACAATAACTTTTCT TTCACAAATCTTTATTTTTGAAATCCTTATGTTATAAGGGTAAATATGGTTTTATACATGCAGCAAGTTATGGCTTTCTT CTACTGGTTGATCATGAAGCTGGGAAAGGATATCAAGATTATTGTTAAACCAACGTTCAAAGATGGCATGACCGTTGGAG TTCAATGGCAACTCGAATGTGACAAGTCACACATCCAATTGGGGAAGGGATTCAGTTTCCACATTTGCCATATGTACCAG GGAAAGCTATTGATAAAGAATGTGGAGATGTTCATGGAACCAATTTTCCACATAGAGCACCTAAGACTAAGAACTATGGC GTTTGCAGTGAGTATTGCTGAGAAGATAGTCACTTTCTTGAGACAAGCAGAAAACACAAGGAGACAAGCAATGGCACTCC TGCTACTTGTTCTTCTGCTGCTTGCTGCAGCCGCTTTCTACGTCACACGTCTGAGATTCTGATAAAAACACAGCAAATAA CATGGAAGATAGCAGGAAAAAAAACCATAGCATAGGGAATAAGATTTTTCTATCAGTAGGATGCAAATATTTCCAACAGG ....................................................N.......N................... ............................N................i...........N...........N.......... .............i................................................i....N............ ....i...........i......................................................N........ ...................N.......N.....N.........................................i.... ......................................................................i......... ................................................................................ Pos Score Pred ------------------------ 53 0.220 - 61 0.322 - 109 0.057 - 126 0.775 Yes 138 0.458 - 150 0.425 - 174 0.547 Yes 223 0.634 Yes 228 0.204 - 245 0.645 Yes 257 0.559 Yes 312 0.317 - 340 0.222 - 348 0.420 - 354 0.246 - 396 0.699 Yes 471 0.716 Yes 562 0.261 - 621 0.048 -

Explain the output -- Processing locus 1104: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATATTAATGTCTCGGAAAAGGAAAATGAGAATTTCCAGATCGCATTTAGGCAAATTCCCAATTCGATTTCTGTATTTG CCCCTATAAATACTCTGTTGATCGACTATTGTTTCATCGGCCCTTTCGTTGGAGAGTGCTGGATCTTCTCCCTTCGTTGG CAAAAATGTTCCGACTTTCTCGAATGCTGAGGCGATTGAACGAGGGATGCCGTTCTTCAATAACCTTCGAGTTCCCTGGA CTCTCCTATTCCAGGCGAGCTCGGAGGAAAGACGTGAAGCCGAGGGTTTCTTGTCATGTGTCGAGTCTAGGCATAAGGAA TACCGCGAGATGCTGAGGTACTATGATGAACGAAGATCTGACATCGCTCGAAAAATGGCTATGCTGGAGTCTCATCCTCG CCATTGGCTTAAGAATGGTTTTCAGCTTCTGGCTCCGATGCCGGACGTTCTTTTGTCGTATTGTGAGGACTGTGATCGAG ATGCTGCTGCCGTCTCAAAGGAGAAGAATCCAACAATCATTGCTGATGGGAACTTCATTCCCAGGACGCCTTCTCTATTC TACCTTGGGCCGGCTGCTACTGATGCTGATACCACGGAGTGTCAGAACTTCGTTAACCAAGTCGGATTTCTGCATCAGGA TCAATGCCGCGAGCTGCAGTACTTTCGAACCAAGAAACGGAGTGGACTTTCGTCTCGAAGAGCTCGAAACTCTGATTGCT AAGCTGAAGAAGAAAAGGAGAAGGGTCGTCACTCGTCTGGATGAGGTCGAACAAACGATTAGGGCATTGGAGATTCGTTC GGAGGATTGGGAGAGAGCTGG ........N.................N..................................................... ................................................................................ .....N.................N......................i................................. .......................................................N........................ .........N............N..N............................i.....i................... ..............N......................i.......................................... N............................................N.................................. ......................N......................................................... ...i............................................................................ ........................................N....................................... ..................... Pos Score Pred ------------------------ 9 0.298 - 27 0.352 - 166 0.480 - 184 0.257 - 207 0.599 Yes 296 0.222 - 330 0.388 - 343 0.229 - 346 0.236 - 375 0.699 Yes 381 0.607 Yes 415 0.327 - 438 0.619 Yes 481 0.175 - 526 0.376 - 583 0.485 - 644 0.659 Yes 761 0.094 -

Explain the output -- Processing locus 1105: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCGAATACAAAAATCTTGCTTTAGATGGGCCGACATATTAGGCCCAATTTGGCAGATAAGCCTAATGGTATGTTAATGAG CCGAAAATAAACTCTAACTGATATCATCGTCAAAAGTTTTATAAGGTTTTTCGCCGGCGACGTACTTCGACGGAGATGGC TCTGTCTTCGTCGACTTTCCAAATCCCGTTCCTCAAACACCGTTACTTCGGAATGTCTCAAAATCTGAGGACACCTATTT TAACGATGTCTACAATCCCCAGAGATCGTTTGATTTCGTCATTTCATACGGCGCGAAGCAAACTCTGTTCTCCTCTCCGG ACCATCAGCTGCGTCGCCGGAGACGATGAAACCAGAGAAGCATCTTCTCTTCCTTCTTCGATATCTTCGCTTTTCGTGAA AGGGTTTTCGGATTCAGTGAGTGAAGGACGTTTAAAGAAAGTCTTCTCCGAGTTTGGACAAGTTACCAATGAGCACTACT TTTCTGGCAGTAAAAATCATCGCAAACGAAAGGACTCGGCAGTCACTAGGATACGGATATGTCTGGTTCAACAGCAAAGA GGACGCACAGTCGGCTGTCGAAGCCATGAACGGAAAGTTCTTTGATGGCAGGTTCATTCTTGTTAAATTTGGTCAGCCTG GTTTGTCCCGTCGCCGGAGACCACATTCCGATTTCCTTTTTGTAAATAAATGATCTTAACCATT ........................N.......................................N....N.....N.... ...........................................................................N.... ....................................................i........................... .....i.......................................................................... .........................N...................................................... ....................................................................i........... ..........................................................i..................... .........................N..................N................................... .................................................N.............. Pos Score Pred ------------------------ 25 0.180 - 65 0.344 - 70 0.241 - 76 0.169 - 156 0.368 - 213 0.559 Yes 246 0.756 Yes 346 0.335 - 469 0.628 Yes 539 0.517 Yes 586 0.355 - 605 0.435 - 690 0.046 -

Explain the output -- Processing locus 1106: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATATCGTCAAAGAACACTTTATATGATTTTTCTCTATTTTAAAGTTTCTTCCTTTTTCATCTAAAAATCGGCAGAAAAT CCTAACTCCAGTCTTGCGTAATTAATGATCGTCTTCCCTTTTATCACACACTCTTTTATTATTTTGCTCAGCAAAAGATT TGATTTTTTTTTTGCTTTTATAGATTCTGGGAATAGTTTAGAGATGGGGAGATTGTTCTTGGTGAATTTGGAAGGCAAGT CTTACAGTTGTAAGCACTGCAAGACCAATCTTGCTCTCTGTGATGATGTCGTCTCTAAGTCTTTTCAGTCCCGACATGGG AAAGCTTACCTCTTCAGTAAGGTAGTGAATGTGTATGCTGGCAAGAAAGAAGATAGGATGATGATGACGGGAATGCATAC GGTGGTCGATATTTACTGTGTCAAATGCGGCTCTTATGTTGGATGGAAATATGAGTTTGCTTTTGAGAAGAATCAAAAGT ACAAGGAAGGAAAATCTGTTCTCGAAAGGTACAAGGTCTGGGGTCCAGATGGGAACAATTATTGGGTGGCTCAAGAAGTT GAAGCCGGAGACAGCGATACTGATGATGCTTGATTCTCATCATTCATATCTGATTTGTACATTCCCTCCAACTCTTTCAT TTTTCTTTATTCTTTTTCCTCATTTTGTAAACCATCTATCTTACATTAGAAC .......................N........................................................ ........................N....................................................... ...........................................i.................................... ..........................................N..N.............................N.... ............................N.....N......................N..i..i........i....... ........................N..........N......N.......N............................. ................................................N............................... ......................N..N...................................................... .................................................... Pos Score Pred ------------------------ 24 0.422 - 105 0.306 - 204 0.597 Yes 283 0.271 - 286 0.203 - 316 0.090 - 349 0.156 - 355 0.184 - 378 0.476 - 381 0.522 Yes 384 0.650 Yes 393 0.537 Yes 425 0.393 - 436 0.254 - 443 0.106 - 451 0.272 - 529 0.429 - 583 0.079 - 586 0.097 -

Explain the output -- Processing locus 1107: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTACCATGTAAAATATATAGTATGATTCTGTGAGAGATGTGGTGGTTAAATCTTAAACGCTTATAAATTTAGAGTAGAT GGTTTTGTTTTCATAACATAAAACGGGTTGAGAGATCAGAAAGCATCGATGGAGCAAATTAGGGGTTTGTATCACTTGGT AGTGACGGTGACGTGCTTGGTGTGTTTAGGATTTAATGTTAGCATGTGTTCAGGATCGGATTCGGATCCCTTCATCAAGG CTTACGTGTCTTCATCACTACCGTCTCACGATGGCTTGGTACAAAGTCTATCTGCATCGGAATGGAATTCACTTGTGATC CAATCTAAAGTCCCGGTAATAGTTGTATTCATAGCCAAGGACTGTGCCGAATGTGGTAGTTTGATGCCCGAGCTCGAATT CCTAGACTCAGAATACGAATATATGTTAAAATTCTACACAGTCGACACTGACGAGGAGTTGGAGCTAGCAAAGGATTACC GCATAGAATATCATCCGATCACTATTGTCTTTAAAGGCGGAGAAGAAAAGGAAAGAGTCCTCGGATATTACCCACAGATG TTAGGTCAGCTCGCCAATAAGTACCTGTGATTTTCCTTGGCTCGTCCGATTATTTATGTTTGCTGTTTCCTTTGTCAAAC GTTTGCTACTACAGCGTCGTCATTTGTCTCTTTCAATTATGAATAAGAC ......N...............N..............N........................................N. ................................................i............................... ...................................N.......N.................................... ..............................N..............................N.................. ..................................................i............i................ ......................i......................................................... .............................................................................N.. .......................................................N........................ ......................................N.......... Pos Score Pred ------------------------ 7 0.241 - 23 0.221 - 38 0.336 - 79 0.151 - 129 0.638 Yes 196 0.123 - 204 0.346 - 271 0.485 - 302 0.320 - 371 0.639 Yes 384 0.689 Yes 423 0.626 Yes 558 0.384 - 616 0.079 - 679 0.057 -

Explain the output -- Processing locus 1108: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CATCTTCTGGAACCAATGGATCCCTGTTACAAAAGTTGCTCTTAAAACATAAACAAAAACAAAAGAAAAGAAAACATTTT TATATTTGTCGTCAATAATAATGGCCACTTTCTGCTTACATGTATATGAATGTATATATTACTATAATACGTAGCTGCTC GTGTTTCTTGTCTAAGTTCTCTGTTATAGTTAGGAGACAAATAAAGAGAATTAGAGAACAGAAGACCAGAAAAATAAAGA TGAACACAACCAACAACGTGTTTGATGTGTCACCGTTCTTGCTTTTAGAAGCATCTGCAGATTCCGAAGCTGGCCACGAC AGCGTTGACGATGATAAATGCGTTAAGGATTACGATCTTGGTCATGAGTCTTCTAGTGCCAGTTCTTGCGAGACGTCTTG CGTGAGTCAGAGAACGAGTTTGTTAGGTTTTGACCTGCAGGACGATACGGTGAATCATGAAGAAAGAGACGCCGGAGATG AAGAAGACGAAGACGGAGAAGGAGAAGTGAATAGCTACATAAGATGTGGGAGAAGCCAACGTGAAAATCTGGCTGTGGAT TCTGCTGCGGTGGTGAGTGAGATGGATCAAAATCGAATGTTCTGGGAAGCTTGTCTCGCATCTTGATCTGTCTTTATTAA TTAATGTCATCTCATCATCAACAGAGAAAACAAAATAGTATATATCTAGCTTAGCGTAGTGTTCTTGTCTGTGTTTCCCC TTTGG ...............i................................................................ ....................i..................i.....N...N.............................. ...............................................................................i ........................N....................................................... ..........N......N.........................N.................................... ........................................................N....................N.. ...........................................N.................................... .....................N..............N........................................... ...N............................................................................ ..... Pos Score Pred ------------------------ 16 0.569 Yes 101 0.689 Yes 120 0.560 Yes 126 0.407 - 130 0.255 - 240 0.692 Yes 265 0.376 - 331 0.282 - 338 0.417 - 364 0.379 - 457 0.215 - 478 0.297 - 524 0.394 - 582 0.232 - 597 0.363 - 644 0.110 -

Explain the output -- Processing locus 1109: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAGAAACTCGGTAATGTGCAAAAAATATCTTACTCTTAATACGTGTAATTTTGGAGTGTAATAGGTCTATCGATCTATAA AACGATACTATTGGAGATTAGATTCTTCTCATCTCACTTTGTTCATCTAAAAACTCCTCCTTTCATTTCCAAACAAAAAC TTCTTTTTATTCTCACATCTTAAAGATCTCTCTCATGGCGATGTCTTTCTCAGGAGCTGTTCTCACTGGTATGGCTTCTT CTTTCCACAGCGGAGCCAAGCAGAGCAGCTTCGGCGCTGTCAGAGTCGGCCAGAAAACTCAGTTCGTCGTCGTTTCTCAA CGCAAGAAGTCGTTGATCTACGCCGCTAAAGGTGACGGCAACATCCTCGATGACCTCAACGAGGCCACAAAGAAAGCTTC AGATTTCGTGACGGATAAAACAAAAGAGGCATTAGCAGATGGTGAGAAAGCGAAAGACTACGTTGTTGAAAAAAACAGTG AAACCGCAGATACATTGGGTAAAGAAGCTGAGAAAGCTGCGGCGTATGTGGAGGAGAAAGGAAAAGAAGCCGCAAACAAG GCGGCAGAGTTCGCGGAGGGTAAAGCAGGAGAGGCTAAGGATGCCACAAAGTAGGGTCTTACCTAATCAGTTAATTTCAA GCACTTAAACTCGTAGATATATTGATCCATATCCTCTCTCTTCATGTTTAATAGTACTTACAATAAGATGAGT .............N.................................................................. ................................................................................ ..................................i.....i.............................i......... ................................................................................ .................................................N.............................. ......................................N......................................... .............................................N.................................. ........................................N....................................... ...........................................N.......................N..... Pos Score Pred ------------------------ 14 0.345 - 195 0.855 Yes 201 0.818 Yes 231 0.765 Yes 370 0.173 - 439 0.443 - 526 0.190 - 601 0.170 - 684 0.045 - 708 0.047 -

Explain the output -- Processing locus 1110: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTTTTATAAGAGCTGGGATGCCCTTTAAGAGTGCTGAATCATATGATAAATTATTATGAACATTAAAATATATGGGCCG AAATCATCTGATAAAGAATAAAGGCCCAAAAGCCCATTAAGAATACCAAGAAATTTTGGAAAATCGAGAAAAATGGTGGA AGACACAAAGACACAAATGGCAGCAGCGATATCAGCGACTTTGGTATTCGCTGCAAGACGACGTCCATTGTTCTGTAGCT TATCATCATCGTCTTCTTCGAAACCAAACACGAGAAAGCTTGTTCTATACTCCAAACCTGGTTGTTGCCTCTGCGATGGT CTTAAAGAGAAGCTTCACGCTGCCTTCACTCTCTCCACCGGTCCTGATTCACTCAACGACGTTACTCTTCAGGTAAGAGA TATAACTACGAATCCCGAGTGGGAACGAGCGTATCAGTATGAGATACCAGTGTTGGCTAAAGAGAATTCTGATGGGAAAG AGGAAGTTTTACCGAGGTTATCTCCACGTTTAAGTGCAGAACTCATTCAGAAGAAGCTACTTGCTGCCTTTAGTTGATCA ATTACGTGCACGACAACGAAAAAAAGAGACAAAAGCGTTTGTAAGGTTGTACTCTCATCTGCAAGAACCGTGTTTTTGCT TCATACTTTCGTTTCT ..................N........................N............N...............i....... ........................................................................N....... ................N............................................................... ...........................................................................N.... ................................................................................ ......................................N................................N........ ................................................................................ ................................................................................ ................ Pos Score Pred ------------------------ 19 0.364 - 44 0.122 - 57 0.469 - 73 0.521 Yes 153 0.479 - 177 0.445 - 316 0.472 - 439 0.300 - 472 0.233 -

Explain the output -- Processing locus 1111: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGAGAGGAAAAAAGCAAACACTTTCACTTGGTGGACCCATTTGTCCCTCCCCCAGACTCTCTCTTCTCATCTCTCTCTCT CTCTCTCTCTTCAAACCCTAAAAATTCACGCATTTTGGCACAGTTAAGTTCGCTGAATCCCGTTCCTCTTCCTCTGTCTC TCTCCCCAAGAAAATCACTCAGATCAAAGCTCAAATTTCGGATCTCACAGCTGTCGCTTCGGTAGGTATTTCCTCTTCTT TTCCCCCCCTTTCTCTTCTCCTGTTTCGTTTTCGATTTGGATTACACGAAATTCATGTGATATGTTGCTTCTTTGGGTTT TGTAAAATGATTGCTGGTGAATTGTATTGGGAATTTTGTGCAGAGGAAAGGATGACCAGAGAACATCCTCCCGAGCCTCT TGATTTCTTCATCTGGACTGTTGAGGATGTTGGGTCTTGGCTCGAGGAGATAAACCTTGGGGGCTACCGCCAGATTTTCA AAGAAAATGGTGTCAATGGAGAATATCTAGAAAGCATGTCTGTGTTTACTACCGAGCAGATCCTTCACTTCATAAGGAGG CATCACATGAAGTGGGGAGACTTCATCACCCTCTGTAAAGAACTCAGAAGGATTAAAGTGGCTTGCTTGAAAGGTGAGCA GAGAGTTAGACGGCCATGGTGGGCACCGTCTTGTCTATCCGTAGTCTTTGTTAAGGCAGCCAAACGCAACCGACAGTCTC GTGTTGTATCTCTCAAGCTTGAATCTTGACATTACACTTTGGCTGTTTATGTACGTACTTGTCTCTCTTCTCTTCTCTTG TTTTCTTTTCCTTTTTTTTTTTTTTTGGTTTGGGTTTGTTTGTGGCAC ................................................................................ ................................................................................ ................................................................................ ......................................................i......N.................. ......i............................................i............................ ..........................N..................................................... ......N........N...................i............................................ ......N......................................................................... ...............N................................................................ ................................................N............................... ................................................ Pos Score Pred ------------------------ 295 0.563 Yes 302 0.254 - 327 0.764 Yes 372 0.749 Yes 427 0.378 - 487 0.405 - 496 0.399 - 516 0.583 Yes 567 0.416 - 656 0.346 - 769 0.064 -

Explain the output -- Processing locus 1112: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Explain the output -- Processing locus 1113: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGCTTTAACGGCAAGTGCTGCAACAGGTATCATTTATTGTTTTCTCGCATAGTCTACCGCTTTCAAATGATACCCCATAT GTGATTTTGAGGTTTTGTTGAGAGATTTTAGCGTTGCGAGGAGCTGCGATTCTAAGGTCACGAATGGAAAGGAACAACGA GAACAAAGCAATGCTATATGCAACAAAGGAGCACGTCGTAGAAATGGAAGAGAGAAATGTATTTGGAGCGATGACTTTCG TCTCTAGAGGAGAAGAGCTTCTCAAACGCACCCGCAAAGGGGATCTTCATTGGAAGCAAGTATCTTTTAACATCAATTCC AATTGGCAAGTGGTTCTTAAAATGAAGAGCAAACATGTGGGTGGAACTTTCACGAAGACGAAGAAATGCGTTGTCAATGG AGTGTGCCGAGATATACCCGAATGGGCACATAGAGGACGAGCAGATAAAATGGTAGAGCGAAGAGCCTACTTTGGGGTGA AAACAGTGGAAAGGGTGATTGAATTCGAATGTGGAAACAAAAGAGAGAAGCAAATGTGGATAGAGGGGATTCAACAGTTG CTTAACTCACTCGAAATCGGTTCTAGTGTTCAATGGAAACATTAGAGTTAGGATCAAATTAAAACACTATTTCACATTTG AACATCATGTAATAACGATTGATTTTTTTTTTCGATTTCACGTGTGTTGAAGAATGTATGTTTG ..................................................................N...........N. ...............................................................i................ ..........i......N.........................i............N.............i......... ................................................................................ .....................i............i..............................i..........N... .....................N...........................i.............................. ............................N........................N.......................... ................................N............................................... ......N..............................................N...N...... Pos Score Pred ------------------------ 67 0.449 - 79 0.351 - 144 0.582 Yes 171 0.696 Yes 178 0.169 - 204 0.605 Yes 217 0.421 - 231 0.529 Yes 342 0.702 Yes 355 0.746 Yes 386 0.713 Yes 397 0.418 - 422 0.456 - 450 0.577 Yes 509 0.369 - 534 0.460 - 593 0.127 - 647 0.087 - 694 0.064 - 698 0.052 -

Explain the output -- Processing locus 1114: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATAGCGAGTAGCCGAGTAGAATGATTGAGTGCGTGCATATTATATATACATACTACAAGATGCAAGTGGTGGAACATAG GTCGTGTATATAAAGAGGTGAAAGGAAAATTTCAAGAAGGAAAACAAAAACGAAACACACAAAGACAAACAGAGAAAGAG AATGGAAAAACAATTGTATCAAACCATGGAGGCTGCTCACATCGAACGAGGCATAGACTCATCGCCAGAAAAGGAGATGG CAAAAACACATAAGCATAGAGGACTATTCCACCTCCACCACCACTCAAAAGATGAGAAAGATGAAGACAAAAAGAAAGAA GGATCAAAGAGAGAGAAGATAGCTGCAGCAATGGTTGGTCTCGGAGCCACACTCAAGAAAGTTAAGCATCCACGCCACCA TGGAAGTGGTGGACAAGAGGAACAAGAAGGAGCAGCAGAGGAAGAAGAAGTAGAAGAAGAAGAGGAGGAGGAGGAGGAAG ATGATGGTGGAGATGAAGAAGAGGGAGGCAAGTTTAGTGCTTTCATTTCGATGATCGCCGAAGCATTTGAAGAATAGAAA TTGTAATTTGTTATATTTTGTTTATTCAAACTTGCTATCAAATTTAGTATTTGTAATGTTTCCTTCTTAGCAAACTTTGT AATGTTATTTTGATTT .....................i......................................i................... ................................................................................ ...................................................N........N................... ..............................i................................................N ................................................................................ N..N........N.....................................N............................. .......................................................N........................ Pos Score Pred ------------------------ 22 0.626 Yes 61 0.635 Yes 162 0.800 Yes 186 0.574 Yes 237 0.710 Yes 292 0.496 - 301 0.484 - 351 0.833 Yes 400 0.349 - 481 0.100 - 484 0.166 - 493 0.074 - 531 0.146 - 616 0.061 - 642 0.061 -

Explain the output -- Processing locus 1115: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CAAACTCAATTTCTTCTCAACGAACCCTAATTTCCAACAAATCGGATTCAGTTTCTTCTTCGGGTATGTTTCTTCTCACC AATAAATTCGAGATCTTTTAGGGTTTTCTGGGTTTGTTTCTCCCCCGATCTAGCAGACGATCGAGTTTATTAATTATGAT CTTTGCTTGCTTGAAATTCGCAGAGACTAATCGAATCGCAATGGCTAAGAGTTCCTTCAAGATCTCTAACCCTCTCGAGG CAAGGATGAGTGAATCTTCTCGAATCAGAGAGAAGTACCCTGACAGAATTCCCGTGATTGTGGAGAAGGCTGGACAAAGT GATGTTCCTGACATTGACAAGAAGAAGTATCTTGTACCAGCTGATCTAACAGTGGGACAATTTGTATACGTGGTTCGTAA AAGAATCAAGCTTGGAGCTGAGAAAGCTATTTTTGTCTTTGTTAAGAACACATTGCCTCCAACTGCTGCATTGATGTCTG CGATCTATGAAGAACACAAAGATGAGGATGGGTTCCTCTACATGACTTACAGTGGAGAGAACACTTTTGGATCTCTTACC GTTGCTTGAATAAAAATCGATCTTTGGATGACTTTGATGTACATACATAAATCAGGAAGATAAAGATGTACATTGCTTCC TTCTTTTCTCTGGCTTTTAACTTTGCTT .................................................................N.............. ...........................................................................i.... ........................................i....................................... .....i.......................................................................... .........................................................................N...... ......N..............N.....N.............N...................................... ...........................N........N............................N.............. ............................ Pos Score Pred ------------------------ 66 0.290 - 156 0.531 Yes 201 0.587 Yes 246 0.832 Yes 322 0.192 - 474 0.437 - 487 0.204 - 502 0.473 - 508 0.405 - 522 0.380 - 588 0.043 - 597 0.061 - 626 0.101 -

Explain the output -- Processing locus 1116: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCTGTATCCCAATTCTCTCCTTCAGAGTTTATTTTCTCTTTGAGAACAAGCTAAAAGCTTAATTTTTTTCCTATCTCTGT GTAAACAAAACATCACAATAGATTCTTCGATGTCAAGAAGAAAACCAAATTTTCTGTGAGAAGAAACCAAACTTGTCTAT GAGAGAAGATACAATGTTTAAGAAAGCCCTCGAGCTTTCAACTCTGTGCGATATCGAAGTCTGTGTCATACTTTATAGTC GTGACGGAGAACTCATCAAGACATGGCCGGAGGATCAATCCAAGGTTCGAGACATGGCAGAGAGGTTTAGCAAACTACAC GAGAGAGAGCGACGCAAGAAAAGAACCAACCTTTCTCTGTTTCTACGTAAGAAGATCCTCGACAACAGTAAGCTCTCGGA GAAAGTCTTAGAGATGAAGGATTCATTAGAAAGTGGTCTACGGGTACTACAAGATAAGCTTCTGTTACTACAACCCGAGA AAAACCAGACCGAGTTAGGTCAGATCCCCGTCATCAACAATGGACAGAACCATTGGTGAATATCGGTGTGTCAAACACAG AGCAAGACCTATCGACGTCACCATTGAGTCAACATCAGAGCAAATTTTCAGTCTTTCTCTATAACCATGACAACTGTA ................................................................................ .............................N................................................N. .............i.................................................................. ......................N..............................i.......................... ................................................................................ .............N.................................................................. .......................................i........................................ ..................................................................N........... Pos Score Pred ------------------------ 110 0.480 - 159 0.462 - 174 0.763 Yes 263 0.440 - 294 0.688 Yes 414 0.497 - 520 0.695 Yes 627 0.062 -

Explain the output -- Processing locus 1117: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATTCCCTATATATAAAATATTTAACTTAATTATGGTGATTTTTTTCTTAATAAGGTTGAAATCCTACTTGGAGAGAAAAA TCTCTTTTAAGTTATATATAAACACTTCCCACGTCTAATTATTCTTCCATGTCCAAGACAATGGCTTTTTCTTGTCTCAT AAGAAATGTATTTTCAATTCTTCCTCTTCTCGTTATTCTATCAATCACCCCTGCGTCTTCATCATTATTGTCTTTGTCTC CAAATGACAAAATTAGAAAAGATTTAGTCAACCAACTCTGCTCACAACCGACAATTTCCAAGCAGTTCTGTCTCGCTTGG CTAACCTCTTTCCCAAACACGTTCACCCTTGACATGCAAGGTCTTGTCAATATGGTCTATCAACAGACGGAACGGTTGGG CTATAAGAACCTAGAGATGATTAAAGGCTTAGATAGAACCGAGAATTATTCGAAGCTCAAGAAGTATTATAGGTCTTGCG TGAAAGAATATGAATTATCCAATAAGGCGATCGAGGAAGCTAAAGGGTTTGCAAGCTCTAAGGCGTACCGGTCAGCTTCT GAGGCGGCTGCTAGAGCCTTTGATAGTATATCTATGTGCGAAGCTTATCTTGAAGGAAGCAAAACCCCGGGTTATGTCAC GACACGCAACTGGTGGTTTGAAAGAATGTGTGACATTGACAAAATATTCACCGACCTTTTGATTTCTGCTAAGTTTTAAT TTACATGTATGATGTGAAAAAACTATGAAATGTTATCAACTTTGGTGTAATAACTTAAAATCCAAATAAATTAATAGTCA TAGTTAAATTAGTTAGTC ...............................N................................................ ................................................N...........N................... .....i.......................................................................... ...N............................................................................ .................................i.................N............................ ................i............................................................... .........N...................................................................... .................................N.......................................N...... .........................i...................................................... ....N...N..N............N....N.................................................. .................. Pos Score Pred ------------------------ 32 0.257 - 129 0.375 - 141 0.388 - 166 0.554 Yes 244 0.470 - 354 0.619 Yes 372 0.367 - 417 0.626 Yes 490 0.448 - 594 0.407 - 634 0.145 - 666 0.524 Yes 725 0.168 - 729 0.086 - 732 0.086 - 745 0.093 - 750 0.053 -

Explain the output -- Processing locus 1118: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAAGACATACTTAAGACATTGTTTAATGCTAATTCTACTGTGAACAGTTTCCTGAAGTTTATGTAAGTTCTTCGATTAC AATCATGAGATGTTTCCTTAAGATATCATTTTCTCTGTGTTGAGTCTATCTCTTGATGTTAGATTCTGCGCTGTTTCTTT AATGAATTTGGAATCTGGCCTCCTCTCTCTTGAGAACTTCAAAGCAGCACTTTCAAGTATGCAAAGGCAACCAAAGTTGG AAGCCGTTAGAGATTGTTCCGTACTGGAGCTTTATCTTCTGGTCTGTATGAGGAGGTTAGAAGTGAAGGAGCAAAGCTCA TACAACTTCATCAGTGTGATGAAAGAGTATAAGGCGATACACGATTCTTTTCATACCTCAGACTACTACGCACAAAATGT CTGCCTAAGGGCATTTGAGCACCTGAGAGAGCGACAAGTTATATGCTATGCAGAGAATAGAGGACAGTCTCAGACAGGAG AGTACCGTTTGCAGAAGCTTTTGATATCAGCCTCTGAGCTTCATCAGGGAATGAGATCTCATGCTTGTTGCCCCGCCATC CTTCTCAAGTTATTGGACCATTGATCTCTGTGTTTTTGTCCCTTGGGAATAAGACAAGTCTCTCAAGGCCCTGTGATATG TATGTTTATTTCATGGTAAAAGCTACTTGGTTTACAAACTGTA ..........................N..................................N.................. ....N....N.............................................N........................ ...............................................N................................ ..................N.........................................................i... ..........................................N....N................................ ..................................................N.........N................... .............................................................................N.. Pos Score Pred ------------------------ 27 0.366 - 62 0.085 - 85 0.195 - 90 0.478 - 136 0.127 - 162 0.410 - 219 0.562 Yes 288 0.204 - 339 0.481 - 397 0.635 Yes 443 0.398 - 448 0.387 - 531 0.345 - 541 0.418 - 638 0.084 - 642 0.039 - 653 0.039 -

Explain the output -- Processing locus 1119: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAAGCCTGCAGAGAAGAGAAGAGAAGAAAGCGTTAAAAGACTAATCAAATAAATTCAAGCAGAGAGTGAGAGAGCGGAGA GTATTGTTTCGTCGCTTGTGATTTGAGAGGAGGAGGAGTGATGGAGAAGGTTTACCAATGAGAGAACAGACCATGTTCAA GAAAGCCTTGGAGCTTTCAACTCTGTGCAACATCGAAGTCTGTGTCATATATTATGGTCGTGACGGAGAACTCTTCAAGA CATGGCCGGAGGATGAATCCAAGGTTCGAGACATGGCAGAGAGGTTTACCAAACTAAACGAGAGAGAGCGACGGAAGAAA AGAACCAATCTTTCTCTGTTTCTACGTAAGAAGATCCTCGACGACAATAAGCTCTCAGGGAAAGTCTTAGAGATGAAGGA TTCGTTAGAAAGGGGTCTACGGGTGCTACAAGATAAGCTTCTGTTACTACAACCCGAGAATCAGACCAAGTCCCTGACTC GTTCTGTTTCTAGTTTAGATTATGTGTTCGTTTAATTGTCTGATTAAGAGTTTCTGTTGAATTGTATGTTTTCCTAACTA TGAATGCTATTTTCTCAATTCTATTTTTGTCTATGAGTCTTAAATTTGAGTTTG ................................................................................ ........................................N................N..............i....... .....................................................i.......................... ........................................................................N....... ................................................................................ .....................N...........................................N.............N ...N............................N..................... Pos Score Pred ------------------------ 121 0.301 - 138 0.396 - 153 0.670 Yes 214 0.673 Yes 242 0.547 Yes 253 0.265 - 273 0.634 Yes 393 0.410 - 502 0.128 - 546 0.051 - 560 0.123 - 564 0.051 - 593 0.048 -

Explain the output -- Processing locus 1120: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAACAAAGAAGTGAAGGATCCCGGAAACATCATGGAAATTTAAAACCAGCTTTGCATTAATTACACGACACACTTATA AATACAAACATGAGATGTTGAAAAGGAAAAGACAAATACAATTCAAAACCAAAGCTCACGACAGTCTCTTATTTATCAAT GGCGAATTTTCTCATCAACAGAAGAACAAGAAGAACAATGAAATCAAAGAAAGCTGAAGAAGGGAAGAGATCGGATTCAA ATTCTAATTCAGACAATAACTTCTCATGCAAGAAGCACACGAAACATAGACAGTCTCCTGGAATCTGTTCTCTCTGTCTC ACCGAGAGGCTCTCTAAGCTATCTCTGGAGTACTACGATTACACTAAGAAGGCAGTTGAAACGGCGACGTATTGCGGCTC AACATCAACATCTTCTTCCTCGTCGGTCTCGTCTTGTTACTCTTCTTCTTCTGTCTCTTCTTGTTCGTCTCCTTTGCAAT ATCGGTATAGAGAAAAGAAGAAGGATGGGAAGAAACAAAGCTTCTTGTTCAGATTATTGCTCGGATCCATCGTAGACTAG TTCTTTTCTTTCCGTAGTTTTTAATTCATTATACTACTTTTTATGAATTCATTATTCTTCGATATCTTTTCATTCGGAAA ATGCAGAGAATCAGCAAAA .................................N.............................................. .........N....N...............................................................N. .....................................i.......................................... .........................N...................................................... ................................................................................ ................................................................................ ........................N....................................................... ..........................................N..................................... N.................. Pos Score Pred ------------------------ 34 0.315 - 90 0.415 - 95 0.494 - 159 0.462 - 198 0.531 Yes 266 0.384 - 505 0.430 - 603 0.055 - 641 0.084 -

Explain the output -- Processing locus 1121: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGGGTTGGTATTTATGTCAATGAAAACGAACTAAGCGAAGGACCCACTTTTTTTTGTATAACCAGTTTTGCCCTCTCATT TACTAAAACTAACCGTCATAACATCGATTCATGTGCTTCTTCTGTGTTGCATTTATATCCCAATTTGCTTCCACCAAATA TCTTCCCTCTCTCGATTTTAAACTTCTTCTTCTCCAAGACCACTTCTCACTTTCTCTCTCTCGCTCTCTCTTGGAAACCA AAACAATGACGATGACTCACCTGAATCGTCTGATCTTGATCTCTCTTCTCTTCGTCTCTTTACTTCTCAAATCCTCCACC GCTTCTTCAACCGTCGTCGACGAAGGAAACAGAACATCAAGAAACTTCCGTTACCGAACGCACCGATTCGTTCCGAGGTT TAACCACCACCCTTACCACGTGACTCCTCACCGTTCGTGTGATTCCTTCATTCGTCCTTACGCGCGTTCTATGTGCATTG AGCTTCAGAGAATCCACCGTAGCAGTCGGAAGCAGCCACTTCTTTCTCCTCCTCCTCCGGAGATTGATCCAAGGTACGGC GTCGACAAAAGACTCGTCCCCTCTGGTCCAAACCCGCTTCACAACTAAAACCATATCCTCAAGTTTCTATACATACATAT ATACAGAAACATTTGTCACATATTTTCTAATTTAACTCGTTTCTCGGATTTGTTCGAGAGAGATTTG .............N.....N............................................................ ..............................N................................................. ................................................................................ .....i.....i.................................................................... ................................................................................ ......................................................................i......... ................................................................................ ................................................................................ ................................................................... Pos Score Pred ------------------------ 14 0.410 - 20 0.272 - 111 0.322 - 246 0.828 Yes 252 0.859 Yes 471 0.534 Yes

Explain the output -- Processing locus 1122: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATAATTTTCACGAAGAAAATATTATAAATCTATTTATTTGTTTAAATTTGGAGATATATACCGATGAATGATTAATTA TGTGAAGCCGTTTAACTTTGATTTTCTCTTTGTAGTCAATATTTTTTAGTTAATGTTACTTGTTTCACACATGGAGTTTC AACTATATTTAAATTTATAAAACGGCTAATCAAATACCTAATTAAAGTATTCAAAGATGTTAAGTTAAAGAAAATCTATA TGATAGTTTCACATTTCAATATTCATATAAATAAGCAAGGATTTTATTGTTATTCATCAATCAAAAAAAAACAAAGCATT AAGCATATAGTTGCTATACTAAGACTTTGTACTGAATTTCTCATTATGTCTATCAAAAATGTGTTTTCCCTTCTATATGT TCTATGTATCATAGTTTCTGTAAATGCTCAATTGCCTCAGTTTCCAGCTCAACTTCCTTTTCCGTTTCCGTTCCAACTGA TTTCTGGATTACCTGATATAACAAAATGTTGGTCATCCGTGATGGATATTCCTGGATGCATTGCAGAGATTTCTCAATCC ATTTTCACTGGAAAGTTCGGTAATTTAGGTCCGGCTTGTTGCAAAGCATTTTTGGATGCCAAAGTTAATTGCATACCGAA AATTCCATTCATTCCGTTATTCCCTCCGATGCTAAAGGAACAATGTTCAAGAGTTGCCGGTGCAACTCCTCCCATACCAA AATAGAGTTTTAACTTATTTTAAAAATAGATAGCTTTTTCAACTTATGTTTTAAAATTTTAGCTTTAGTACATGTTATAA TAATAATAAAAAAAGATTATATAG .................................................................i...N.........N ....................................................N.................N......... ........................................................i......................N ................................................................................ .............................................N............N.................N... ...N...................N........................................................ .........................i...............i.............N........................ .......................................................N........................ ............................N.............N..................................... .............................................N.........................N........ ........................ Pos Score Pred ------------------------ 66 0.517 Yes 70 0.231 - 80 0.186 - 133 0.116 - 151 0.158 - 217 0.526 Yes 240 0.312 - 366 0.396 - 379 0.357 - 397 0.117 - 404 0.434 - 424 0.166 - 506 0.555 Yes 522 0.832 Yes 536 0.211 - 616 0.130 - 669 0.122 - 683 0.347 - 766 0.039 - 792 0.042 -

Explain the output -- Processing locus 1123: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAGGAAATTGCGGCGATCTAAGGTATGCAATTTGATTAGAACCCACCACTTCAATTTTTTTTACACCCTATTCAAAGCC AATTTGATTGAAACTAATTAAAAGATGCTATGGATCGTGAAGGTTAAATCCATGGCTGATGCACATTTGAAGCAAAGAGG CTATATCTCGGCAGTTGCAGCTGGACTCAATGCAGCTCTTGCAGCAATCTCTGCTAAGTTCTTCACATCTCTTGTGATTA AGTATGGATTAGTTGTAATCTGCAATGTGGTTATGTGGGCATGTTATGTAAACAGTTTAAGAGCTTTGTCCTCTCTACAA GCTACTGTCACAAACTTTGCTGCTAATTTTCTATCTTCTGGTTTAGCTGGTCTCTTTCTTTTTCAAGAATCGTTATCGTT TCGTTGGTTTGCGGGAGCTTTATCGATTACTACCGGAGTTCTTATCCTCAGTAAGTCGAGTGTCGATAAGAAGGTAAGCT CGGATTAATGGAGAATCGAATATGATAGCTTCTGTTTTGGAGAAGGCTGCTTTTGGTTGAAAGGAATTAGAGATGTTAAG TTGTTCTGAGTTTTGAGTTTTAGAGGT .........................N...................................................... ........................i....i.....................i......N..................... .............................N.................................................. ...N....................N.......N.......N....N.................................. ................................................................................ ................................................................................ .......N.............N..................................................N....... ........................... Pos Score Pred ------------------------ 26 0.350 - 105 0.783 Yes 110 0.584 Yes 132 0.619 Yes 139 0.189 - 190 0.087 - 244 0.314 - 265 0.442 - 273 0.288 - 281 0.126 - 286 0.112 - 488 0.345 - 502 0.104 - 553 0.048 -

Explain the output -- Processing locus 1124: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGGTTTCCATAAAGCCCAATTTAGTTGGCCCAATAGCTTGCAAAACTGGGCGTAACGGATAATTTAATAAAGACACGGTA AATGCTTAGATAGAGGATTAGAGGGTAATTAAATTAACCACGATCACTGTGATAATTACTACAACATTAAACGACAAAAA AACTTTTCGTCTCCCTCATAATCTTCTACTATATATTCGTCACATCACACTCATAATCTCTTACAAAAAATCCATAACAC AAAAAGAAGCAATGGCTACTAAATCTACTTCGAAGCCTCTTCTGCTCTCATTTCTAATGATGTCATATCTCATATCGACC TTTCATGTCATCACTGTCGCCGAGGGAAGGACTCTCCAGTTCACGAAGATGGCTACGGATCATTCCGGCGCCGGAAACTT GATGGACTGTTGGAACGCGGGGTTGGAGCTTAAGTCATGCACCGATGAGATTGTCAAGTTTTTCCTTAGTCAAACCGGTA CGAGTGAACCGCCGGTTAAAGGTGGAATTGACAAAGATTGTTGTGGAGCCATTGGGTTGGTTGTGAAAGATTGTTGGTCC GTTATGTTTACTTCTTTAGGGCTTACGACTATGGAAGGGAATAACTTGAGAGAGTATTGTGAGTTTCAAGCTGAGAAGTC GGAATTATCTCCTTCACCGGCGCCGGAAACTTTGGCTTTGTCTCCGGTTGAGATAACGTATCCCGGACTTGATTATTGAT AGCTTTCCCTTTATATTTTAAGTTAAATAAAAGTTACCAGTGAACTATGTTTCCTTTTTATGTGTAACATTTTCTGATTT ATCTTTGTTAAATTTTGT ................................................................................ ................................................................................ ...........i............................................i..i.................... ....N...........................................i............................... ................................................................................ ...i..........................N................................................. ................................................................................ ..............................................N............N.................... .................. Pos Score Pred ------------------------ 82 0.118 - 252 0.875 Yes 297 0.501 Yes 300 0.843 Yes 325 0.269 - 369 0.629 Yes 402 0.319 - 437 0.265 - 445 0.266 - 564 0.563 Yes 591 0.237 - 767 0.068 - 780 0.038 -

Explain the output -- Processing locus 1125: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTTCCCTTTTAACATTTTCTTTTATTACTAAAATTCTTTCGACTGTGGATTAGTTTATTTGACTTTTCTCATTCCCTCGT CCTCATTTTCATTATAAATAGAGAGCATGCATGGGGACATATTATTCATCACAAGTTTTAAGCAGTATTTGTAAGAAAAT GGCTTCCAAGACTTTGCTTCTTTTGGGTCTCTTTGCATTTCTTTTCATCGTCTCAGAAATGGCTGCCGCGGGCACGGTGA AGTCAGAGAGTGAGGAAACTGTGAAACCTGAACAACATGGTGGTGGATTCGGTGACAATGGAGGAGGAAGATACCAAGGA GGAGGCGGCCATGGTGGCCACGGAGGGGGAGGATACCAAGGAGGAGGAGGACGATACCAAGGAGGCGGTGGGCGACAAGG AGGAGGGGGAAGTTACTGCCGCCACGGCTGCTGCTACAAAGGTTACCATGGCTGCTCAAGGTGTTGTTCATATGCCGGAG AAGCGGTTCAGACTCAGTCTGGTCACTAAATCTACATAATATATCAACCACCATGAGTGTACGATTTCATTTATATATAC ACTTACGTGTGTATGTGATATGACAATACACTTACGTTTGTAATGAAG ................................................................................ ..........................N...N...............................................i. ..........................................................i..................... ....................................i....................i...................... ..........i..................................................................... ...............................................i.......................N........ ....................................................N........................... ............N......N......................N..... Pos Score Pred ------------------------ 107 0.482 - 111 0.278 - 159 0.761 Yes 219 0.836 Yes 277 0.855 Yes 298 0.888 Yes 331 0.894 Yes 448 0.713 Yes 472 0.432 - 533 0.143 - 573 0.074 - 580 0.038 - 603 0.039 -

Explain the output -- Processing locus 1126: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAATGTTACCAGACAAAGGTAACATACAATTTATTTAAAACTCGCACGAAAAAACTCCATTCTCTTACAATACTTTTACA AAATAAAAATGTAACAAATAACTTCCATACATTGGCTATATAAACTCTTTTAGAAAAACTTCAACATACCTTATGTATTT ATTTGAAGTATCAATATATAAAATTGAAACAAAAGATTCTTTCCTTCTCGTAAAAGAAAGAAACAAAAAAAGAAATCATT CGTCGACTTTATTACAAAACCCTCTCACAACACCATCACTTCTTCTTCGTCTTCTGTCTGAGTCCAAAATGGAAGACTAC AGATCCAGATCGTACGGTGACGGGAGAACATCAGACCTTCAACAATACTCTGCTCACCGAAGATCCGACGGTCCAGATTC ATTCAGTGGTAACGGTATGCAAGATCTTAGGTCTTACAGTACTTCCTACACAGATTACCCGACCCGGATACCCGAAGACC AGAACCCGAAGAAAGGAAGATCATCTTCATCGTCTTCTTGGGGATTTGTGGATCCAGATTTACAAAGGAAGAAGAGAGTT GTTAGTTACAGAGCTTATACTGTTGAAGGTAAGCTTAAAGGTTCTTTCAGAAAAAGCTTCAAATGGATCAAAGATAAATG CAACAAATTACTTAATTAATTCTGTCAAATGTGTTTTACATAAGATAATAAACTTTTGTCACTTCGATCGGTTGAGTTTC AATCTTTTTTTGTGTATGTGTGTGTGTGTTTGTGTGTG ..N............................................................................. ........N...............................................................N....... ................................................................................ ....................................................................i........... ................................................................................ ................N............................................................... ................................................................................ ..............................................................N..............N.. ............................N................................................... ...............N...................... Pos Score Pred ------------------------ 3 0.387 - 89 0.379 - 153 0.297 - 309 0.662 Yes 417 0.298 - 623 0.247 - 638 0.227 - 669 0.180 - 736 0.043 -

Explain the output -- Processing locus 1127: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GCAGACAGAGCACACGGTATTTAGATTGCTATTCTACTATATAAAGAGAGCTCATCCTTCTCATATATCTCAACATATAC TTACTCAAACATATTCTATAAAAATTTCCCAAATTAAATATGCTAATTTACGCATTGGATTTTTTCATCCGATTTCCTCG AAAGGATGACTCACAGATCATGGAATTGTGTATAGGCACGGTTAACAACGGCACAAGTGACAAGACGGCGGACCAAATCA AGGCGACAATGGAAGCGGCTTACCGTGTATTATATAGGCATGCACCTTTACCTCGCAATATATTTGGTCCCATGTTCCCG ATGTCTTACCTCCATGTCGCAGTGAGCTCGAGGGTTTTGCACATACTCTCTAAGGTACCGGAGAAAGTAGTAGAGAATGG GTGGAACAAAAGGAAGGCGTGGATAGAAGGATCTGATCGACAAGTGTTGATGCGTTACGCGGAGCAAGCGGACAAGGACT TAGTCGACTTTTTGGCAGAGCCGTGCCGAGGAATTTTGATGCCATAGGCGAAAAAATATGGTTTAATAGTTTAATAAAAT AATGTATACATAACAAAAAAATATTTTCATGTTCAAAGTTTAATAATAAATTACGAAAATCTTAAG ................................................................................ .......................................i........................................ .....N.............i............................................................ ........i..............................N...............................N........ i............N..............................................................i... .................................................N.............................. ......................................N..................N...................... Pos Score Pred ------------------------ 120 0.696 Yes 166 0.290 - 180 0.778 Yes 249 0.650 Yes 280 0.197 - 312 0.457 - 321 0.695 Yes 334 0.215 - 397 0.764 Yes 450 0.162 - 519 0.076 - 538 0.099 - 562 0.155 - 589 0.057 -

Explain the output -- Processing locus 1128: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence ATCGAAATTCTCCGTGTTATTTCATCGTCTCTGCCATCTTCACTGTCTCGCGTCGCCGTGTTCGATTGAGGTACTCCCAC TGCTCCGATTTGAGTATTTAGGGTATGTGTATGAGTGGATTTGACGGTATTGTGATATATCTCAGTTTGGAGTTCTCTCT CTCTGGTCTAAAAGAAATCGTGGAGAGTCCAATGGATCCGCAGACGCAGAACACTTCGTTGCAGCGACTCCAGAATGTTG AGAATAGAGTTGTTAAGGTTTTGGAACTAGCTGGTGGAGTGATGGAAGAACTCGCGAGCCCTTCTGGTCCCAAGAAAGAG TTCGTCAACAGCCATTGCCGAGAGTTTATGCAATCTATGAAGGATATACAAGTGACACTAAGGGAAGAGATCAAAAGCGC TTGCGAGTACCGTCCCTTTGAGAAATGCGATTACAACGCGAGAATAGCCAATGAGATCTGCTTCCAGAAGCTTGAATATG TTCTCACGCAGCTTGAAGATCTGAAACAAACTGCTGACCGGTATCCTTCTTCTGATTGATATTGGTATTCATAAAAAGGT TACTTGAAGAACTATACTAAACCATCGGTTGTGATATAGTTTCCAGATGAGTTGTAGACTTGTAGGTTATCCCATTAA ................................................................................ ........................i.....i................................................. ...............................i..........................................N..... .........................................i...................................... ...........................i........i........................................... ........................i.........................N..........................N.. ................................................................................ ..............................................N............................... Pos Score Pred ------------------------ 105 0.583 Yes 111 0.627 Yes 192 0.806 Yes 235 0.292 - 282 0.613 Yes 348 0.517 Yes 357 0.768 Yes 425 0.557 Yes 451 0.296 - 478 0.374 - 607 0.056 -

Explain the output -- Processing locus 1129: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TCAAAGAAATTAATAACCACGTTGAGGAGTGGTGAGAAGTGGAAAGATCCAAAAACATTAAAGCCACAAACACCAATAAA TTAAAATCTTAAACGAAATAATTGGTACGTTACGAAGTGGAAAAGATCCACCTCACTAATCTATATAAACAAATGCAAAC AAACAAAATTATCACAGATTTGAAACTTATTAATATTTCAAAAAAACAAAAAATGGATCCCAACAAAGCTGAAATTAGTC GTCTTGAGGTACTTCCTCAAGATCTACTCGGAGAAATAGTTGCCAAAATAGGGGCAAAATACGCTGAAGATTATCACAAT TGCATCCTTTCATGCAAAGAACTAGGTGCATCAGCAAACGATGAACGCGTGCTCAAAACCCTTAATCTTGCTCCGTTGGT GAAGAAACCGTTATCATGCCGCAAACACCTTCTTATTATGAAGAAATGTCTTGCCAACAACAACCCGGATGCTCACTACA TAAAAGGTATAATATGGTACTTTAACCTTGATCATTGTGACGTTGGTCTCCACCATATTGGCATAGCAGCTAACTGAGGC CAGAAAGAAGCAATCTACATGTATGCCATGCTATTATTATGTAGAAGAAGGACTGAAGAGGGAAAAACCTATATGAGCCA ACTTGAATGGGCTAAA ................................................................................ ........................................................................i....... ....................................................i........................... ................................................................................ ...........N............................i....................................... ...............N.....................N.......N......................i........... .............N.................................................................. ..................N...N....N..........N.................................N....... ......N......... Pos Score Pred ------------------------ 153 0.508 Yes 213 0.901 Yes 332 0.344 - 361 0.514 Yes 416 0.247 - 438 0.319 - 446 0.297 - 469 0.543 Yes 494 0.231 - 579 0.141 - 583 0.090 - 588 0.072 - 599 0.049 - 633 0.052 - 647 0.052 -

Explain the output -- Processing locus 1130: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTTAAAAAAGGAAAAGAGAAAGGAGAAATAATACAGAATGCATATCCAAAAAGAACAATCATAGAAGATTATTAATATT AAGGGAAAACTTTTATTTTAGATGAGAAATAAATTAGACAAACATCAAACAACTAGCAAAAATATGCAAAAAATGTTGAA TACGATCATGATACCTTTGATTATCATGCTTTCAATATTTATACAAACAAAAGGAAATGATCTTGCACCAATTCCATCGT CGGTATCACCAGAACCTCAATCAGAGAACCAATTCTTTCTTGACCCACTAACTTGTATTCACGATGTGAAAAAGGTTCCA AAGTGTATGGACGCAGTGTTGCACTTCCGATTCAAACAGGTCACAAAGAAGTGTTGTTATGTCATATTCTCTCTTCCCGA AAGTTGCTTTGGGTTTTTGTTTCCTATTCCTTATCTCTACCATCCTCTTCTTAAGGCTGCTTGCAAATTACTTACTCACC ATGGATAAGATTTTTGTAATTCTTTAATAATGTCAATAATCTGCAGTGATAACATCTATAGAAAATATTATAACTTATGT ATAGCCATAATTTAACCTGTATTTTTG ......................................N......................................... .....................N.........................................N........N....... .......N.................N..............................i....................... ...............................................................N................ ......N...................................................N..................... ................................................................................ N............................N..............................................N... ........................... Pos Score Pred ------------------------ 39 0.477 - 102 0.100 - 144 0.389 - 153 0.402 - 168 0.477 - 186 0.203 - 217 0.544 Yes 304 0.163 - 327 0.316 - 379 0.212 - 481 0.158 - 510 0.175 - 557 0.065 -

Explain the output -- Processing locus 1131: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAGTCCTGGAATTAATCTTTAGAGCCAGTAAATTCTCTCTGTTGTTGGAGACAAGATAACTGTGCTTGAGTTTGGTTTAT TAGTAAAACCCAAAACCCTAGATCAACAAAGGTTCATACTTTATAGATTTCCCACTAATGTTGTATCTTGTTTTGTATTT AATCAAATACACTACAACCTATATTGATTTAGTTAGAAGAGAGGAAAAACAGGTTTTAATGATGATCACTATAAAGTTCG ATCTGAATCGTTACTCGTGTTCATGGGGGGCAGGTGGAGCATTTGGAGGAAACAGAGGATTAAGACCAATACCACCAGAG AAGGGCATTTTCCCTTTGGATCACTTGCACGAGTGTGATGCGGAGAAGAAAGAATATCTTGGTTGTCTTAAGTCTTCAGC TCACAAATCTGAACAATGTAGACATCTCTCTAAGAAATATCTCCAGTGTCGAATGGCTAAGAACTTGATGGCAAAGCAGG ATATGGCTGAACTTGGATTCAGTGGTGTTAAAGAACTTGATTCCACTGAAGATAAGAATACAGAGAGTATCGAACATTGA AAGGTTGACAAAAGAAAACTTTGAGCCTCATTTTGTAAAATTTTGGATTAACATTGGGATAGCATTTGAGATTTTAGGTC CTAAAACCCAACTACTATG ................................................................................ .........................................................N...................... ..........................................................i..i.................. ......................N......................................................... .....................................N.......................................... ...............i....................................i..............i............ ..i............................................................................. ................................................................................ ................N.. Pos Score Pred ------------------------ 138 0.233 - 219 0.568 Yes 222 0.543 Yes 263 0.500 - 358 0.449 - 416 0.550 Yes 453 0.862 Yes 468 0.600 Yes 483 0.776 Yes 657 0.036 -

Explain the output -- Processing locus 1132: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATTTTAAATTTTCTGGTTATAAAAGACGAACCAACCTATTATTGAGTCACAATCAAATTGTGGGAGATGAGTCTCTAA CGATAACCTCTCTCTTGGTGACACGTGTTTAGACCACCGCTGCTTAATAAACCCAATCGCCCACCGGAACATGAGTGGCG GCTATACTTCCTCTCTATTTCTTCTCTTCTAAGGATTTTTCGTGTCTCAAAACTTTCAAAGACTCTCAGTTTCTATCTCT GTGAATATAATCTTAGTTATAAAAAAATGGGTCTCTCGCATTTTCCAACAGCGTCAGAAGGAGTACTACCACTTCTGGTG ATGAACACGGTTGTTTCAATCACTCTGTTGAAGAACATGGTGAGGTCTGTTTTTCAAATTGTTGCATCCGAGACTGAATC TTCCATGGAGATAGACGACGAGCCTGAAGATGATTTTGTTACTAGAAGAATCTCGATAACACAGTTCAAGTCTCTATGTG AGAACATAGAAGAGGAAGAAGAAGAGAAAGGTGTGGAGTGTTGTGTGTGCCTTTGTGGGTTTAAAGAGGAAGAGGAAGTG AGTGAGTTGGTTTCTTGCAAGCATTTCTTCCACAGAGCTTGTCTAGACAACTGGTTTGGTAATAACCACACCACATGCCC TCTTTGCAGGTCCATTCTCTAGTGGTGGAATCTTGTTTTACACTTTCCTTTTGTTTTGCTTTGCTATAGGAAAATACCAC ATTCTCTATCCTCTGTTTTACCCTTTTTGGTGTTTATGTTT ....................................................................N........... ......................................................................N......... ................................................................................ ..........................i..................................................... i...................................N........................................... ....i........................N.............................................N.... ................................................................................ ..........................................................................N..... ................................................................................ ...................................N..... Pos Score Pred ------------------------ 69 0.316 - 151 0.386 - 267 0.854 Yes 321 0.717 Yes 357 0.355 - 405 0.515 Yes 430 0.134 - 476 0.184 - 635 0.201 - 756 0.038 -

Explain the output -- Processing locus 1133: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCACACTTCCAATCCTCGACCTTATCCACCTTACGTAGACCTAATATCCAGTCGCCACGTCACACACAATCAACGTCTCA GATGTACCCGATCGCCTTTAAATCATCGCTTCCTTCATGAATCGAAGAACCTCTACAACAACAGATTTTAAAAAACCCCA AAAACTTTACATTCTTAACAATTTCAACCCTATGGGCTTACTTGATCATCTCTGGGATGATACTGTCGCTGGTCCACGGC CAGAGAACGGCCTTGGCAAGCTTCGTAAACACCATACCTTCAGTTTCCGACCTAGCTCCGGCAATGATCAATCGGAAGCT GGAAGTGCGAGATCGTACGGTGAGGATTCGCTACCGGAAGAAGCAGTGAAAGTTACACGTAGTATCATGATAATTAAACC ACCGGGTTACCAAGGCAGTTCAGCTCCAGCTTCGCCTGCCGGTTCAACTCCGCCGCTATCACCTTTCTCTCCGCCGCTGT CTCCGTTCTCTGGTGACAAACGCAGGGGGAAAGGAACCCTTTCGGTTTAGGAGACGGTCGACGTCGGATGCGTTCGAGAA GGCAGCAGGAGGATCAGAGACTGGACCAAGGAGCTCTCCTCCTACTTACGGCATGTGATCTCTAACACA ................................................................................ ...............................i........................N....................... ...............................................................N................ ..................................................................N............. ................................................................................ ...................................................................N............ ....................................................N................ Pos Score Pred ------------------------ 82 0.385 - 117 0.347 - 192 0.500 - 217 0.435 - 304 0.198 - 387 0.260 - 548 0.230 - 613 0.033 -

Explain the output -- Processing locus 1134: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TATTAATATTCCATGATTGTCATTTACTTCATAAGTCAATTTATCTTAAATTCTACTACAAACTACCATAAATTGGTCAA AGATTTATAAAATAAAACTAAAATTGTATGAAAATAAATAACTTTCACAAAAAGTTACTCATATTATATATAATGCAGTA TCATTTGAGTTATGAGTTAAAAGCAAACACCACAATGATTTCGCAATTGACACTAATCTTCTTTTTATCTCTCGTTGTAA TCCAACCTTTTCATATTTTGGCCAAGACATGGTGTGTAGCAGCTGCATCAGCGACAGATACACAACTTCAAGCTAATATT GATTGGGCATGTAACGAAGGTAAAGTTGACTGTGTAAAAATTAATCCTGGTGGCGTTTGCTATGAACCAAATACTCTTAC TAGCCATGCATCATTTGTGATGAATGATTACTATCGGAATCATGGGAGCATTGAAGAGGCATGTGAATTCAATCATACTG GTCAAATCATTAGTGGTGATCCAAGTTATCGTCGTTGTAGGTATTCGTAGAATACATGATTTGATGTATGATTTTGTGAT TGGAAATTTTTCTAATAAACAATAAAATTATATAGAATCAAAAGTATTTTTAAACAATTTGTAAGATTC ............N................................................................... ...........................N............................................N....... ...........N......................i............................................. ............................N................................................... ........N....................................................N.................. .....N.............i...N.................N..................N................... .......................................................N.......N...N............ ..................................................................... Pos Score Pred ------------------------ 13 0.375 - 108 0.176 - 153 0.234 - 172 0.114 - 195 0.681 Yes 269 0.300 - 329 0.135 - 382 0.222 - 406 0.275 - 420 0.745 Yes 424 0.327 - 442 0.488 - 461 0.108 - 536 0.052 - 544 0.064 - 548 0.038 -

Explain the output -- Processing locus 1135: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CGCCAAATAACACGACGAAGCAAGCAAAACACTCTGCCGCCTCCATCGCCTGCCACGTGTACGACGATTATACGTTTTTA TCCATTAAACCGGTTTACTAACGATATATTAAAATCAATTCTCGGTTTGAAATGAAAGAGAGTGGACTTAGACTTAGAGA GATAACAGATAAAATGGCGACGGGGACGGCGACGGAGAAACCGAAGATCGTGTGGAACGAAGGAAGACATAGATTCGAGA CTGATGATCACGAAGCCTTCATAGAGTACAAGATGAAGAATGACGGTAAAGTCATGGATCTGGTTCGAACCTTCGTACCG CCGTCAAAAGCAGGACTTGGTTTAGCTTCGCATCTATGCGTCGCCGCTTTCGAACACGCCTCTGAACACTCTTTCTCCAT CATCCCCACTTGCTCCTACGTTTCCGAAACGTTTCTTCATCGAAACCCAACGTGGCAGCGTCTGGTTCACTCAGAGGATG ATTCCAAGAACCTCAAGTCTAGCATCTGATCAAGTCCTGCTTTAAAGCCTTTTGGTGTATAATAAAAACTTGAAAACATT ATCTTACAATAGTAAAGCTTGTTTCAAGTAATCTCTGTAAACAAACAA ................................................................................ ...................................................i............................ .............i.................................................................. ...N............................i......N.............i.......................... ...................................i............................................ .............................................................................N.. ................................................................................ ................................................ Pos Score Pred ------------------------ 132 0.629 Yes 174 0.887 Yes 244 0.211 - 273 0.743 Yes 280 0.478 - 294 0.615 Yes 356 0.501 Yes 478 0.452 -

Explain the output -- Processing locus 1136: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CCATTTTCATTTTTCATGATTTTGTTTGAGTGAAAGTGTTGGAAATGTACTACAAAGTTTTTAAAAGGAGGATATACACA TATATATATGTGTTGCTTTGAACCTTCTTGGATATGTGTGCAAGTAAATATTGTATCTGTTTCATTGCAGATTCTAAATT TGTATCTGGAAACGGTAGAACCGAGCTGGTTAAATTCATTGCTGATAGGCATGCCAAACTCATGAAACAAGCCTCTCGAC ATTATTCCGCAATTAAAGATGCTATGGCTCGTGGGAAAAAAAGATATACACTTGTAAAAGATGTAGACGACATGGAAACA GGAGCATATGACAAACCTCTTCCATGCTTTGGTTGTGGAATCGGATGGTTTTCCTTCCTCCTAGGATTTATGTTCCCATT TTTGTGGTACTATGCTGCATTCCTTTATTTCGGGAACTATTATCGTAAAGATCCTAGAGAAAGAGCCGGTCTTGCTGCTT CCGCAATCACTGCGATGGGCTTCTCTCTTGTCTTACTCGTGATTTTTGCTTTCCGGTGGTTTTACTATCCTTGAGATAAA GAAGTAACTATGTTAACTATCATTCATACCTATAAGCTCCGTGTTCATCAAGGAATAGTCTCGGAACTTGATGTCTCATG TACATTCACATAA ...............N............................N................................... .......N.........................N.............................................. ..................................................N..........N.................. ..................N....i....................................N..........N........ .......N...............N....................N........................N.......... ...........N.................................................................... ..............N................................................................. .........i............................................................N......N.. ............. Pos Score Pred ------------------------ 16 0.151 - 45 0.278 - 88 0.346 - 114 0.435 - 211 0.230 - 222 0.450 - 259 0.224 - 264 0.688 Yes 301 0.482 - 312 0.211 - 328 0.157 - 344 0.233 - 365 0.148 - 390 0.365 - 412 0.297 - 495 0.367 - 570 0.576 Yes 631 0.057 - 638 0.049 -

Explain the output -- Processing locus 1137: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AATTTATAGTAATATATTCACCTATATAACTGTTTATCCTATGTTTTTCAAAAACTAATGCATTTTCATTTTCTGTAACC TGATTAATATTGATGAACTAACTTTGGCAGATGACAAGCATTTTTTTTTTCATGAAACATTTGACCAAAACTCGCTTCAA TTGTTTTATATCAAATCAACACCAAACTATGATACCAATTACCAAAATATTTTCGTCACTTCAACACCACAAGACCATAA TTCAAGAATCAACACGTTGCTTCGCTAATTTTATTCATCTTGTAGTTATTTCACCAATGGTCCCAAAAAAAATCATTGCT GGTTATAATTGTAAACAAATTCTATTTGTCACGTCAAAAAGATATTTTTTTGTCTTGATGTGGTTAGACCCTCTTCTATA TAATATCATCTCCACACACAACTCTCTTCTCAAAAAACACCAAATCATTCAAGAAACCAAAACTAAGGAGAGGAAGAAGA AGATCATGTCAGGCGTGTGGGTCTTCAAGAACGGAGTGATAAGACTTGTGGAGAACCCTAACCAGTCAGGATCCGACACA CAGAACCGAAGGAAAGTGATGGTCTATTTACCGACAGGAGAAGTGGTCTCATCTTACTCGACGCTCGAGCAGATCCTCCA GAGTCTTGGATGGGAGAGATACTTTGGCGGCGGCGACACAGATCTCCTCCAATTCCACAAACGCTCCTCCATTGATCTCA TTTCCCTGCCTAGAGATTTCACCAAATTCAACTCCGTTTACATGTACGATATCGTCGTCAAGAACCCTAATTACTTCCAT GTCCGAGACTCCCATTGATTCTTCTTTTGTGTGTTGGTGTGTGTGTTGTGTTCGGTTGTTTTATAATTTAGAGTTTGTTG GGTTTTGTTTTATAGTTGCATGTGCATGTTTTATTTG ........................................N................N...................... ............N.................N....................N............................ ............................N................................................... ........................................................N....................... .........................................................N...................... ................................................................................ .....i.......................................................................... ..................i............................................................. .........i...................................................................... .........................................N....................................N. ................................................................................ ...................N.....N........... Pos Score Pred ------------------------ 41 0.230 - 58 0.306 - 93 0.106 - 111 0.166 - 132 0.080 - 189 0.428 - 297 0.329 - 378 0.428 - 486 0.556 Yes 579 0.621 Yes 650 0.614 Yes 762 0.352 - 799 0.450 - 900 0.040 - 906 0.040 -

Explain the output -- Processing locus 1138: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AGGTTTAAGACAATATGAAATGTGATCAATTGAAGAAATAAGAAATATAACAAATCCGGCTGGAGAATACTGGACTGGAA CTGAACTAAACCGAAGAGTAAACCAAGACAAAACCGAAGATTGAAAATCATTGGCTTGCTAGTAAACTACTACGAGACAA AAGTCTACTTATCCCCCCACTCATACCACTAAAAAACTCACACTTGCTGGCTTCTTCTTCCATGGCTTTATCTTTTGTTT CTTCAACTAGATTCTCCAAGATGCAATATGGCATATGGCACAGTGGATCCTTCACGCAACGAGTCAGTGTGAGATGCTGT GAAATCGCAAACGAGGCTCCACGACCAAAATCGAAGCTCCAAGTAGGATCACCGATCATCATCGTCGAAGCTCCCAAAGT TATAAAAACGGCCGCTTCAATGCCTTGCCTTAGGGCCAACTCTGGCTTGGTCAAGCCTGGCGATGTTGGAAGAATCGTGT CGAGAAAACCAAAGGACTTGTGGGCAGTTAGACTCTCCATTGGAACATATCTTTTAGATGGTAAATATTTCAAAGCTTTA GAGCTTGACGAAGGAGATAGTGATTGAGAATTTGAGATCATGTCTCTAAAATTGTATAAGCAACAGAAAAATACGAATTA TTATTTTAACATTTTGCCATTTTAAGTTTTGGCGCAACAATCTTAT ..............i....i............................................................ ................................................................................ .............................................................N.................. ....................i......i......i......................................i...... ................................................................................ ...................i..........................................N................. .........................................................N...................... .......................................N........................................ .............................................. Pos Score Pred ------------------------ 15 0.614 Yes 20 0.729 Yes 222 0.378 - 261 0.634 Yes 268 0.859 Yes 275 0.580 Yes 314 0.619 Yes 420 0.517 Yes 463 0.356 - 538 0.381 - 600 0.080 -

Explain the output -- Processing locus 1139: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GAATTGTTCGTCCGTAAAATCAGTTTTGGAATTGAGCTGATAATATTAGAAGATAGATATAGCTTATCTGAAGAGAAAAT CGCATAGAAGAGTAGATCTGAGATTTTGTTCTTGAACTAAATATGTCACGATTATTGAAATTGGCAGAGAAAATGGTGGC GACAGGCTTATTCGTGGGGCTAAACAAAGGACACGTTGTTACCAAACGCGAGCAACCTCCTCGCCCTAACAACAGAAAAG GGAAAACAAGCAAAAGGACTATTTTTATCAGGAATCTCATCAAGGAAGTTGCGGGTCAAGCTCCCTATGAGAAGAGGATC ACTGAGCTTTTGAAGGTTGGTAAAGACAAGAGAGCTCTTAAAGTTGCCAAGCGAAAGTTGGGAACTCACAAGAGAGCTAA ACGAAAGAGAGAGGAGATGTCCAGTGTTCTCCGCAAGATGAGGTCTGGTGGTGCTGGTGCATCCGAGAAGAAGAAGTGAT GCGCTGACTCTGGTTCAGCGCTCTGTTTCTTCTAAACCAGTTTTCTGTTTTTTGAATTTTTTGCAGTACCTTGTGTTTCC TTTGGATATTTTGTAGCA ................................................................................ ..........................................N.............................i....... ................................................................................ ..................................................................N............. ................................................................................ ................i....................N........................................N. ................................................................................ .................. Pos Score Pred ------------------------ 123 0.477 - 153 0.852 Yes 307 0.180 - 417 0.502 Yes 438 0.193 - 479 0.210 -

Explain the output -- Processing locus 1140: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence CTGGTTAACACAACAATGTCATGACCTTCCTACCCACAAATGCTTACTGCTTTATAAATATACTGTATTTCTGAAAAGAA ATTGAGAAAACAAGAAGTTAAGAGATAGCAATGCTTCATCAGGGAAGCCGATTCTCTAGCTTGATTATACTCTATGCCAT TTTCTCTTTATCTTCTCTCATGCTAAAATCTGAAGGAACAGAGCATATAGTTGGTGACAGCAATGGCTGGGAACTCTTTA CAAACTATACCAACTGGACACAGGGAAGAGAGTTTCATGTCGGCGATGTCTTAGTATTCAACTACAAAAGTGACCAGCAT AATGTTATGCAAGTTAACTCTACAGCATACACAGATTGTGGATTAGACAATTACACAACTCTCTTCACCAAAGGTAACGA CTCAATAATCCTATCAGAAGTTGGAAAGCTATGGTTCATCTGTGGAGTGGATGATCACTGTGTAAATGGCCAGAAGCTAA GCATTAATGTAGCTCCTTGAGTACCGTAATGATTATCAAAGATAGGAAGCTTGTTGTTTGGGTAGTCTTTAGTACCAAAA TAATGTCATTAACATTGATAATATCATCTGAGTAATATA ...............i....i..................i........................................ ..............................N..........................................N...... ...................N..........................................i................. ....................................N........N.................................. ..............................N...................N..............N.............. ......N.....................N................................................... ..N.................................... Pos Score Pred ------------------------ 16 0.516 Yes 21 0.591 Yes 40 0.713 Yes 111 0.367 - 154 0.316 - 180 0.342 - 223 0.616 Yes 277 0.368 - 286 0.432 - 322 0.286 - 327 0.352 - 431 0.325 - 451 0.084 - 466 0.455 - 487 0.325 - 509 0.140 - 563 0.071 -

Explain the output -- Processing locus 1141: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAAAGAAAACATTTAAAACTCTGTATACATCATACATGACATCTTTACAAATTGCAAAATATACATTTTTAATCAGCTAC TCTATTCTTAAATTCCTTTAATTAACGAGGTTATACAGTTCGATACGTTTAAGCAACAAAAAGTTCGATGCCACTAATAA TTTAATTTTTGTTCTCTTCTTATCTTATAGATTGTTGCAGCCTGCAGGCTTGCAGCATAACGAGTTACATAACTATATAC AAAGGCCCAATAGACACCTCAAAGGCCCAATAAAAAAGCCCAGGAAAAACGAAAACGAAGATATGAAGTTCTTCTCTAAT CCGAAGCAAGAGGCTCAAACTCACTTTCACTTGCACAAAGATAAGGAAACCATGTCTGTGTCAGCGATCTTTGGTACCGG AATCGTCACCGTTGCTGCTTCTCCGGTTCTCCGCCAATTTCAAGTTCCAAAATTGGGTAATGGAGGAGGATTAGGGATGG TGATAGAGTGTTCGTCGAGGCCACAGAAGAAATCGACGGCTCATCACAGGAAGACGAGGCCGAAGAAGACTCAGCCTTGG GACATTAAGAGAAAGCCTACTGTTTACGCTCCTCTTCCTCCTCTTCCGGCGGAATGGAGTCCGTTCACTCTCGCTTCTGA CGACGGTGGTGCTGCCACTGCTGCGGGAGATTTGGTTTCAGGCGCTGCCTAGTTGGTATGAGTTATCTGCTGATTTGGTT GTAATCTAGTTTGAGAACTTTTGTTGTTGTTTACTGTGTTTTCAGTTTTCTGATGTTTGTTGTTGGGTTTG ...................................N............................................ ...................................................................N............ ................................................................................ ..............................................................i................. ...................................................i............................ ...........................................................i................i... ................................................................................ .....................................................N.......................... .........................................................N...................... ....................................................N.................. Pos Score Pred ------------------------ 36 0.240 - 148 0.471 - 303 0.621 Yes 372 0.776 Yes 460 0.783 Yes 477 0.606 Yes 614 0.255 - 698 0.104 - 773 0.058 -

Explain the output -- Processing locus 1142: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence AAAAAAAATGGAGACAAAAAAGGAAATTCCATAAACGCGTTAGAAGTTAGAACAAATTATAATCAGATCTATGCTTATAA AAACATAACATTACACTTTAATTCTCCACGAATCATATTTTCTCTCTGATTTTGTCGAATAAAAATTCGACAATGGATAC TGAATTAATGAAACTCGATGTTCATGTAACCGCTTGGTCTCGTACTCCATCATCGTCAGGTTTCTTAAACTCGGTAATGA TCAGTCGAGACAGAGACTTCGAGGAATTTATCATAAACGACAGGGATGGTAGCGTCACGAGTCTCGGATCTTACCAGAAT CCTTCTCGAGGTCCCGACCCACTAATCTTTCTAGACTTCAGAAACTTCGAACCAAACTACGTCTATCAACTTCTCCTAAG CCAATTCCACGATCATGTCTTGTCCGAACATATAGCTGATCAGATTGTCCAAGCCAAACGGGATAAGAAGTCAAAAATCT GACTTGCCACAAGAACCTTTATTCATGATGGTCTCCGTCAAGTTGACACAGAAGGGATACACTTTCGGGACTTGTAACTC TCCTACCTCGACAACAGATTT .......i..............................................................N......... ........................................................................i....... .......N.........N.....N....................................................N... .............................................i.................................. ................................................................................ ..............N................................................................. ........................N..N.................................................... ..................... Pos Score Pred ------------------------ 8 0.605 Yes 71 0.322 - 153 0.847 Yes 168 0.356 - 178 0.265 - 184 0.219 - 237 0.401 - 286 0.601 Yes 415 0.378 - 505 0.196 - 508 0.081 -

Explain the output -- Processing locus 1143: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence GGGACCTACATGTGGGACTCCACAACAAAAGTATCTACACTGTACACTTTTTTATTTTGGTGCGAGAACCTCTCATGGTT TTTTTTCTTAACAAAAGATAGAACATGACACGTGGCCACAACCCATTTGCACGAAAATCATATTTGGATAAGGAAATGGA TATCATTTCTCCACAATTCCACAAACTCAAATTACCCAACTTCTTAAACACATTCCCATCACCTCACAAACCATTAACCG AGAGCCAACTAGCGAGTAGAATCAACTATCAAGCAATGGCATCGATGACCATGACCGCTACCTTCTTCCCGGCCGTCGCC AAGGTTCCCTCAGCCACCGGCGGACGAAGGCTCTCCGTAGTCAGAGCCTCCACGAGCGACAACACACCCAGCTTAGAAGT CAAGGAACAGAGCAGCACCACAATGAGGAGGGACCTCATGTTCACGGCTGCAGCTGCAGCGGTTTGTTCTTTGGCTAAGG TAGCCATGGCTGAAGAAGAAGAGCCCAAGCGAGGGACAGAGGCGGCCAAGAAGAAATACGCACAAGTTTGTGTCACAATG CCTACGGCCAAGATCTGCCGCTACTGATTCAACAACCCACCTTCTGCATGTCTATCTCTCTCTTTCAATTTCGGTAAATT TTCTCAATTAAGTTTGGTCTGTAATCGTATATTTGATCATCAATTT .........N................................................................N..... ........................N..................................................i.... ................................................................................ ...................................i........i.....i............................. ................................................................................ ......................i..............i.......................................... .....i.......................................................................N.. ...............................................N................................ .............................................. Pos Score Pred ------------------------ 10 0.274 - 75 0.463 - 105 0.314 - 156 0.512 Yes 276 0.905 Yes 285 0.795 Yes 291 0.846 Yes 423 0.865 Yes 438 0.774 Yes 486 0.504 Yes 558 0.171 - 608 0.106 -

Explain the output -- Processing locus 1144: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTTATAAAAAAACAAGAGTATATTTAGACAATAATTTTATTAATGGCTTCTACTCTTCTAGTTTGCAAAAAAATCAGTGA CAGGAGAAATATATTTTCTAGATTAAAAAGTATTTGGAAATTGGACTATATATAGTGGACCCCTATCATTTGTTCATAAT GAGCTTAGTCTGCAAAATATATAATCTACCTGAGCTTCATTACAATCATTTCTGTTTTCAGAAAATGGCAACAAAACTTA GCATCATTGTTTTCTCCATTGTTGTGTTACATCTTCTTCTGTCTGCCCATATGCATTTTTTAATTAATGTTTGTGCTGAA TGCGAAACTAAATCCGCTATTCCACCTTTATTAGAGTGCGGGCCAAGGTGTGGAGATAGATGCTCGAATACACAATACAA GAAGCCGTGTTTGTTCTTCTGCAACAAATGTTGTAACAAGTGCTTGTGTGTGCCCCCAGGTACTTATGGCAATAAGCAAG TATGTCCTTGCTATAACAACTGGAAGACCAAGAGCGGTGGACCAAAATGCCCTTAGTTTCTCCTCTTAATTACTTTAGCA TAAACTCCATGTAATTTGTTAATCTACCTATCATAATTTATATATGTATTGGACTCTTCCATAATCACATCAGTT ..........................................N..................................... ..............................................................................N. ................................................................i............... ..................................................N...............i............N ...........................................................N.................... ...........................N.....................................i.............. ........N..................................N............................... Pos Score Pred ------------------------ 43 0.392 - 159 0.354 - 225 0.800 Yes 291 0.386 - 307 0.537 Yes 320 0.431 - 380 0.326 - 428 0.301 - 466 0.660 Yes 482 0.410 - 527 0.402 - 569 0.054 - 604 0.053 -

Explain the output -- Processing locus 1145: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTGGGGATATTTTCTTAAACAAAAAGCAACATTCATTAAATTTTGCTTTCACTTTCTAAAAAATCTTGGCATATTAATAC ATTGAACATGTCTTCTTATAAATGAACCAAACTAAACCAAAAGATCCTAATCAAAATCAACCAAACATGAGCAAATCAAT CTTTGTTGTTTGTATCACTTTATTGGTTGTTCTATCCCCAACTCTAAATGCTAAAATGGTGACTTACCCGAATGGTGATA GACACTGTGTAATGGCCCAAGGCCAAGTGATAAGTGCATGCTTGCAACAAGCCAACGGACTACCACATGCGGATTGTTGC TATGCCATAAACGATGTAAACAGATATGTAGAGACAATCTACGGTCGTTTAGCTCTTTGCAAATGTTTCCAGGAAATTCT AAAAGATTCTCGATTTACAAAACTTATTGGTATGCCTGAAAAATGTGCCATTCCTAATGCTGTGCCTTTTGACCCCAAAA CGGATTGTGACAGGTTTGTAGAACATATTTGGCTTAAAATGTTTTGAATTATTTGCACTATCTCGATAGAACTTTAAGAT TTTCTTTGTAAATAAAGTTCTGAGAAGGTTAAAAAATTGATGGTTATGTAAAATTTCATTGATTTT ................................................................................ .......N.............N............................................i............. ...............................................i.......i...............N........ ...........i.........................N............................N............. ...............................N..........N.............N....................... ......................................N......................................... .......................................N.....N.................... Pos Score Pred ------------------------ 88 0.221 - 102 0.329 - 147 0.611 Yes 208 0.500 - 216 0.543 Yes 232 0.408 - 252 0.802 Yes 278 0.267 - 307 0.492 - 322 0.443 - 334 0.247 - 346 0.390 - 383 0.232 - 432 0.493 - 443 0.498 - 457 0.292 - 519 0.256 - 600 0.074 - 606 0.037 -

Explain the output -- Processing locus 1146: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTCTCTTTTCTTTGTTGTACGTATGATATTAAAAGTAGAGGAATATGATTCTTGAGTGTAGAATCTTGCCTTCTACCATG GTTCAAGTGTAAGCAATATAAAAAAGGGAGATGTGTGTTTATTTCTTCCCAACTTTGATACTCACAAGACATCTTTTTAA TTCACTCCCTTGCAAAATGGTGGATCATCATCTCAAAGCTGATCGTGTAGTTGCTCGGGTAGAGAAGTCGGTCAATTTGG TTATAGGTCATAGGCAAACACAAAGGCAGGCCGGCTCAAGGGAGTATGAAGGTGAATCAAAGCAGAAGTACACAACTAAG AAGATCCCAAAATGGAAGCATAATGATTCAGAGAGAAAGATAGGTCAAAAGCAAAACAATGAAACACCAACTATGATTGT CGCTGGTGGATGGGTCAGGCCAAATCGAGCCATGTGGGCTTCACCTCCAAATTCCTCGAGCTCCAAGCCACAAAAGAATA ATTAATTATTGAGAGAAGCTGGTGGTAACATATATAAGAACTCAAATTAAATGTTCAAGTTGGGAAGTAGACAACTGTTG AGCGAGAACAAGGCCAGTCGTTTC ......................N.....................i................................N.. ..............................N................................................. ................i............................................................... .............................................i.................................. ...........i..........N...................................N.............i....... .........N.....................i................................................ ..................................................N............................. ........................ Pos Score Pred ------------------------ 23 0.095 - 45 0.507 Yes 78 0.172 - 111 0.335 - 177 0.801 Yes 286 0.678 Yes 332 0.582 Yes 343 0.351 - 379 0.469 - 393 0.674 Yes 410 0.414 - 432 0.761 Yes 531 0.111 -

Explain the output -- Processing locus 1147: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TGTTTCATGTCTTTACATGGTCTTTTTCTTGAATCCTAAGCAATATCTCATTGTCAAACTGCTCTACATGTTGAAATGCA TGTTTCTTTTTCATTATCTGACGCACTTTAGTCTTGCATCTACTCGTGCAGGTGGACCATGCGTCTCCCATGCAACAAAA GACATTTGGCTTGGCTCTTTCCATAATACTTACACTTAGAATGCCCCAAGTTCTTGACAAGCTTGATCTAATACTAAGTA CATGCACCAGTGTGATCCTTGGTGAAAACAAAGATTTAACAGAAGAAGAATCAAGGTCTCAAGGAGAAGAAACCCCGCCA AGCAAAGAGTTACGGAAAAGTCAGATAAAAGTTTCCGACCCAATCTACCAGATGTCACTAGAGAACTCAACGCGTGAGAA CCTTCAGACATGTTCTACACTTCATGGAGATGCCTTTAATTCTGCCATTAGTAGGATGCACCCATCGGCGCTTGCACAAG TGAAGCAAGCCTTAAAGCTGCCGTGAATAAATGTTTTTGCCTCGTGATTGTTTGTTTCCTTATCAGTTAAAGAGCAGCAG TATTCAAGTGAGTTTCTGATTCGTTGTTATTCTCTCTTTTCAGCG ......i.........N..................................................N.......N...N ..........................................................N..........N.......... ........................................i....................................... ...................................................N............................ .........i.............N.....N.........................N........................ ..............................N................................................. ............................................. Pos Score Pred ------------------------ 7 0.530 Yes 17 0.267 - 68 0.316 - 76 0.374 - 80 0.313 - 139 0.353 - 150 0.315 - 201 0.718 Yes 242 0.406 - 372 0.462 - 410 0.651 Yes 424 0.156 - 430 0.155 - 456 0.265 - 511 0.091 -

Explain the output -- Processing locus 1148: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TTATATATCTTTTTTTTTTCAGACGGTAATACTGTATATAATCTCCTTAAAACCATCACATATTATTATATAAACAAATT GATTGTGGATTCTCTTAATAATTATATTTCTATATGGGTCAAACAAATAATTCATTAATTTTCCCAATCTTTCCTGTCAA ACTTTATTATATAAGATTCATTCCATACAATCCTAAATTCTTTTCTATTTATATACGATACAATACCTTATCATCTCCAC ACAATCCAAAACCACAAAACAACAAAAAAATAAAAATATACTCAAAAATGAAACTACAGCAAACCAAAGTAATGTTTTTC TTGTTAGCACTAATCTCAACATTGATGTTCCAACCATCGGAAGCTCATAATACGAACCTGTGTCCGACAACAGCGATCGA TAACGTGCCTGGATGTTTCGATGCGGTGAGAAAAGCGGCAGCCGGAGATTTCCGATGGTTCACAGAAGTTTGTTGCAAGG CAGTAAGAACACTTCCTGATACTTGCTTGTTGCTTGTTAATCCTGGCCAAGCTTACCCTACTAATATCTTCAGAAGCATT TGTATTGGCAAGTTTCCTCCATTGCGTCATTAATTTTCTTTATCTTTTAAGTGTTAACCAATAATAAGATGAATTACTAA TTTACTCTTTAATTTTTGTACTTCGTCTCATTCGAAGTTTCGTTTATAAGAA ................................................................................ .................................N.............................................. ................................................................................ ...............................................i.......................i........ ........................i....................................................... ............N.......N.................................N......................... ................................................................................ ....................................................................N........... .................................................... Pos Score Pred ------------------------ 114 0.387 - 288 0.601 Yes 312 0.621 Yes 345 0.619 Yes 413 0.333 - 421 0.193 - 455 0.237 - 629 0.106 -

Explain the output -- Processing locus 1149: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATGTAACCCATCTCATAACAGATTTATAATTTCGTGTATAATATAATCTCCAAATCACTAATTATGACCTCACATTTT AGGATAATGTTTTTTTTTTAAATGCTTATGGTCAAGAAAGAAGATAACGATTCAAGCCACACGAAACAGAAGAAGACAAT GGCCTCTCTCTTCACACCATCACCAACCACCACTAGTAGTAGTGGAGACACCATCTATGTAGCGGCGGTGCCGCTTAAAG CCGCGGCCGGTCCGCCGCAACTTATTATGTCAATGGCTTACTCACTTAATATCTCAAATTTACAACATTTCATGGTCCTC ATCAAACCTTCTTCTCCAATCCGTCAAGAGGTTCTTGTGTTTGATTTTCAGCCACGTAACCCTGAAAGCATAGAAGCAGC AATCTCACTTTTGTCAGGAAATTTGATTCCAGGTTCTGATTCTCAGACATCGAATGTGTAGTTTTGCAAAGAAGGTTGAA GAATGTTCCAAGACAAAGATGTTGGATGGTTGGACCATCTAAAGGGAATGATGCTATGGAAATGGCAATGGAGTTTAATA ..N..............................................................i.............. ......N..............N.....i..................................................i. ........................................................N....................... ..........................N.....N......................................i........ ................................................................................ .....................................................N.......................... ..N...............N......N.....................N..N....N.....N.....N............ Pos Score Pred ------------------------ 3 0.122 - 66 0.783 Yes 87 0.233 - 102 0.423 - 108 0.577 Yes 159 0.872 Yes 217 0.198 - 267 0.157 - 273 0.450 - 312 0.626 Yes 454 0.305 - 483 0.081 - 499 0.085 - 506 0.072 - 528 0.030 - 531 0.041 - 536 0.068 - 542 0.071 - 548 0.063 -

Explain the output -- Processing locus 1150: NetStart 1.0 Prediction Results

NetStart 1.0 Prediction Results

Translation start predictions for 1 dicot plant sequence Name: Sequence TAATGTAACCCATCTCATAACAGATTTATAATTTCGTGTATAATATAATCTCCAAATCACTAATTATGACCTCACATTTT AGGATAATGTTTTTTTTTTAAATGCTTATGGTCAAGAAAGAAGATAACGATTCAAGCCACACGAAACAGAAGAAGACAAT GGCCTCTCTCTTCACACCATCACCAACCACCACTAGTAGTAGTGGAGACACCATCTATGTAGCGGCGGTGCCGCTTAAAG CCGCGGCCGGTCCGCCGCAACTTATTATGTCAATGGCTTACTCACTTAATATCTCAAATTTACAACATTTCATGGTCCTC ATCAAACCTTCTTCTCCAATCCGTCAAGAGGTTCTTGTGTTTGATTTTCAGCCACGTAACCCTGAAAGCATAGAAGCAGC AATCTCACTTTTGTCAGGAAATTTGATTCCAGGTTCTGATTCTCAGACATCGAATGTGTAGTTTTGCAAAGAAGGTTGAA GAATGTTCCAAGACAAAGATGTTGGATGGTTGGACCATCTAAAGGGAATGATGCTATGGAAATGGCAATGGAGTTTAATA ..N..............................................................i.............. ......N..............N.....i..................................................i. ........................................................N....................... ..........................N.....N......................................i........ ................................................................................ .....................................................N.......................... ..N...............N......N.....................N..N....N.....N.....N............ Pos Score Pred ------------------------ 3 0.122 - 66 0.783 Yes 87 0.233 - 102 0.423 - 108 0.577 Yes 159 0.872 Yes 217 0.198 - 267 0.157 - 273 0.450 - 312 0.626 Yes 454 0.305 - 483 0.081 - 499 0.085 - 506 0.072 - 528 0.030 - 531 0.041 - 536 0.068 - 542 0.071 - 548 0.063 -

Explain the output --